LOCUS       DAOQII010000001        47160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_316_length_47160_cov_5.597155, whole genome shotgun sequence.
ACCESSION   DAOQII010000001 DAOQII010000000
VERSION     DAOQII010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 47160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47160
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_47160_cov_5.597155"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLF48_00005"
     CDS             <1..388
                     /locus_tag="PLF48_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNF47729.1"
                     /translation="ASAPKWGKMNVGQMLAHSNVTYEYALDERDDKPNFLVKMMLKNF
                     VKNKVVSETPYTESMPTGPAFVIKESKDFDKEKTRLINYINKVKSLGAAHYESKESSS
                     FGVLTAAEWNNMMYKHIDHHLKQFGV"
     gene            complement(567..2057)
                     /locus_tag="PLF48_00010"
     CDS             complement(567..2057)
                     /locus_tag="PLF48_00010"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNF47730.1"
                     /translation="MSKQIFIFDTTLRDGEQVPGCQLNTIEKIEVALALEELGVDVLE
                     AGFPISSPGDFNSVVEITKNISKPTICALTRAVAADIDCAAQALKHAKRARIHTGIGS
                     SDIHIKNKLKTTRENILEKAAWAAKYARNYVDDVEFFCEDAGRADLEFLARMVETVIA
                     NGATVVNIPDTTGYCLPEQYGAKIKYLMNNVPNIDKAILSAHCHNDLGLATANSVAAI
                     ENGARQVEVTMNGIGERAGNTSLEEIAMILKSHKNNLDLECGINIKKIYPTSRLITRL
                     MRMPVQPNKAIVGRNAFSHSSGIHQDGVLKNRENYEIIDPHDVGIPESLIVLTARSGR
                     AALKHRMENIGFNLDQAQLDETYARFLDMADNRKEIYDEDLLELMGQSREGKIYEIDY
                     LEVHCGKRHQATATVALMKEGKRTVEIADGCGPVDATLKAIDKIVKHSVRLEEYLVQA
                     ITGGSDDTGKVHIQISKDGVFYYGFGNDTDVIYATAKAYVDALNKI"
     gene            complement(2167..3990)
                     /locus_tag="PLF48_00015"
     CDS             complement(2167..3990)
                     /locus_tag="PLF48_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF47731.1"
                     /translation="MKQILKYCRPYLSNIALNILFNLLSTLFSIFSFLMLIPFLNLIF
                     NSDKIQVSQTPVSFSLYNFKAFLNYSLNVRLLYYIQDTADGKTKALLFICLLTVAIFL
                     LKNIFRYLAVHFLAPVRTGVATNIRKQLYEKILFADVLYFSDKRKGDIMTRLTNDVQE
                     VEYGILHFIEVAFKEPVTIIATLVTMLLMSPKLTLFVLIILPVSGYIIGKIGKSLKQA
                     SAKAQQLQGDINAQVEETISGLRIIKSFTAEKLFLNRFNKITQHHYQLTTSMIRRRDL
                     STPLSEFLGIGVVVIVLYVGGKMVINHTSSLSAEAFIAFIVIFSQIIQPAKAFSNAFY
                     FIQKGLASLSRIETVLHTENKIKDVPKAISKTNFDEQIVFKNVSFRYENEDVLSGINL
                     KIQKGKKIALVGVSGSGKSTLIQLLLRFFDVSSGEILVDNIPIKDIKQEDLRGLTALV
                     TQHTTLFNDSVQNNIALGHTGTETTIRQAAKQAFADDFIERLPDKYQTNIGDNGSKLS
                     GGEQQRLTIARAIYKNAPILILDEATSHLDSNAEKLVQDALQHLLENKTAIIIAHRLS
                     TVLFADEIVVLKQGKIIEQGTHAELTALMGEYKKLVDLQQL"
     gene            4038..4439
                     /gene="rbfA"
                     /locus_tag="PLF48_00020"
     CDS             4038..4439
                     /gene="rbfA"
                     /locus_tag="PLF48_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNF47732.1"
                     /translation="METIRQRQIAKTIQLALSEVIQQDLSFDLQGALVTIAGVKMTPD
                     LLTARIYLSIFNHKNPDELLEYFDAHNRTIRGLLGKKIRNKVRQIPALEFFKEDSLDH
                     VFEIEQLLKDVKEKDEEIAKYRTDEDESADA"
     gene            4432..5670
                     /locus_tag="PLF48_00025"
     CDS             4432..5670
                     /locus_tag="PLF48_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF47733.1"
                     /translation="MLNLELNIAKRYLFSKKSTNAINIISLVSAFAMLFGTMALILVL
                     SVFNGLEDLVKSLYNVFYSDVAVFPAKGKTFEITDELKAKLKAVDFIEASSFVLQENA
                     LLEYEGNQHICTIKAVDDNYFKVAKGFDTFIVDGKKILYADSINYIIPGVGVAQGLGL
                     NNIMTLNPVGIYMPKKSASNFALLENAFNRNYLTPSAVFAVSDEFDTKYALTSLSFFQ
                     NLTENYDKASEIDIRLKNPKLNEKAIQSIKNKLGIGFEVKSRYEQNEVLYKILKSEKW
                     VTFSILVFIILIASFNIIGALSMLVLEKKKDIATLTALGFKQRSILKLFLYEGLLMSG
                     LGAICGMVLAVILCILQQKIGLIPMPGTSFLVKYYPVKMNLSDFAVVSFVIIAISLIA
                     AWFPAKKAAKSVENEYLTYL"
     gene            5683..6219
                     /locus_tag="PLF48_00030"
     CDS             5683..6219
                     /locus_tag="PLF48_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47734.1"
                     /translation="MKSKSFFILLLFCINLGFGQMRSKLDSAINTTGYLCKYEVLQTA
                     NFKSLRFDAVRYSNLENFSVLSGLKFIYKIQSGKELKSMHAYIDQKDVEGLLMTLQYM
                     RTMLKSKTMPANYTEIKFTTTAGFQVMLYTILNDKNNLSWKFSVIANVQNDKSYLECT
                     TDDIVILEEALSSLKNKI"
     gene            complement(6240..6875)
                     /locus_tag="PLF48_00035"
     CDS             complement(6240..6875)
                     /locus_tag="PLF48_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNF47735.1"
                     /translation="MTKIQIFVYEIKKSWSEEKFANALHTLPEFMQKIILEYKHRQTA
                     MHSLVGKLLLNYAIKKLNLNFALQDVRMTTKGRPYFASNIDFNIAHSGNWVVLAVAEK
                     RIGIDIEMHRTIQIDLFRKYFSDEEWGNIQKNENPGQQFFDYWAIKESAIKCNGKGVE
                     VLGKTVVLNDFQIICEGETLYYKALKIDNTYSSALTCTSDFIYEIEKVNAF"
     gene            complement(6868..7569)
                     /locus_tag="PLF48_00040"
     CDS             complement(6868..7569)
                     /locus_tag="PLF48_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF47736.1"
                     /translation="MKAILLHHAGGDRYAFKRVEEKLSAHFATKSIELPGRGDRFDEK
                     RLTNFEFALHDMLTHVEPEINEPYFFVGVSMGVVFAYELTHLLQAEGHPLPRALFLAS
                     RRSEEDYSDLQRFALSEKEVFWEHILTYDDKSRQLLHHPEILEVIEPVLRADFSILED
                     YHDTYALKPKLPVPAYILYGAEDLHKNNEQTSANWQKHFTINIEVKIFDGGHFFLYEN
                     EKAAAYIISKLKGND"
     gene            complement(7650..9320)
                     /locus_tag="PLF48_00045"
     CDS             complement(7650..9320)
                     /locus_tag="PLF48_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47737.1"
                     /translation="MLKRLENFLVSIFAEDVPLKQTTPIWQLIAGIGVLVYCGLHIYA
                     GLENHMDIQFADEAAYLRFGLDLPGHLNRDWGPLYAIWYKLLSFFTTDTIKLYYLNFR
                     LTAIIPPILLLLFLWRMGVNFILAFFISYCVLVSEANISVWPRVSQFCIILLLSGLIL
                     STYFKNKFYKLLLLTLVTLLCSYARPEFYLAFILLLFITILLGFYQPITFNKKTTGWI
                     IFYVIIIASLHLFFRFPSNNFFGFNRGVAAFYQHYAFNLRIQHKLDVDAWLGWEDLAK
                     STFGNCNSMLCVIINQPKIFILNTLFNIKNYIAFTFVSVGSYLFPLVLFHSNKIKFLI
                     AFSSYIGLILLLINKTTRNKIKTQFSHYIFYILFLLVFISPTILSCVFVFPREHYIYL
                     QMLFFVLIIISILNPLLPEKINVKAILLGLFLICLCVCPSYSKYPFLKMDSANAKLCN
                     KKMIRLLQQNYNDKPHTIFTNMPFVRGMLPENFHEVNTIFDKKKLIPYAHYADSAKID
                     IVIVNASLLRDPHIIYDSTWISFFNNPTARGFKPLHFSECETYLLIKE"
     gene            complement(9323..10936)
                     /locus_tag="PLF48_00050"
     CDS             complement(9323..10936)
                     /locus_tag="PLF48_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47738.1"
                     /translation="MQLLKKQIYFISGMAFMLFCGIKWSLHLSSNMDILFGDEAEYMR
                     NGLDLFKIIRNDWGPAYNIWYKFLSFFKSDTIQLYYLNYALGSVLIGLLFFVFLYQHI
                     KQKQVALYVGFCFLVSSININTWPRVSHFVIIILLTSLILASLTSSNAKKWLIYSIAC
                     FICAYARPDLMLSFFCCVPVTFYFIWKEKNAIKSFLPYLFILLIVVLFFQFVFGQPSA
                     TYKGGLDRLYSAFCQHYAMNYKMMHKDGFDAITEWINFSKQQFPNSHTLKEVILNQPG
                     LFLQNLLLNCKNYLMILLLTVTSFVFPTGIFKSKKALILSVFILLLLVVVVFANKKYR
                     DQFFVKLQDNKQLLLFLFIFGVPSLGMSIVIFPRMHYVLLHIILLAFIIALLLDSITL
                     EINMQWSYWLFFLLFIVISPKSSSYAYMQFGSDMKNLCEQKLIRFLEKDKTHPHVVMT
                     NYLNVTYILPKNYSEFSTEFELKEGMSFRQILDERKIDIILVSVNIIKNPILLKDTAW
                     NQFMRNPEPFGFNKVEYSNECESYFLIKQ"
     gene            complement(10949..>12324)
                     /locus_tag="PLF48_00055"
     CDS             complement(10949..>12324)
                     /locus_tag="PLF48_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47739.1"
                     /translation="NYQVTAVLCALLLFIFLRNYSIHVIIAFWLSFCFMFSTTIMDTW
                     PRVSHFAFILLVLGFIAIRNCKTYLSKSVWLTAVFFITAYARPELFPSFLLMCLLTIF
                     IYFKFRESLRSALPAFVFLIVIVIVFFGIYQLPADSYKGINRTYIAFCQHYAINYVLE
                     TKANFNPISEWIDFAHRTFGDCKNFSCILKSHPGLVLHNTILNIQKYCLVLLEFFTSI
                     LFPVKITAKKWLMMLIWFFMIGILVYVFLNKETRNKFIQSLKDNKLVLLVLLIFGLPS
                     MATSLLIFPRAHYLLMHAPLLIFLVALLINALPVFTKSKSYLLLLFFIPFIYKSPKAN
                     KYTYYQMDRDNKQKCNRELVNYLTEKYNHHQHVIFSNHLSLSMMLPENYADFNTEYEF
                     KPGMHFSAILKDKKIDHVLMRDILLQDKLLQQDTSWTSFVAHPEDYGFQKQIYCDSCE
                     SYLLVKP"
     assembly_gap    12325..12424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12425..12711)
                     /locus_tag="PLF48_00060"
     CDS             complement(<12425..12711)
                     /locus_tag="PLF48_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47740.1"
                     /translation="MKNNALFIVGLLLLLFCGIKWALGLPAVMDITFGDEAQYLRYGT
                     DLFHTIHKDWGPSYNIWYKLLSLIQHNNIRLFYFNYQVTAVLCALLLFIFLR"
     gene            complement(12747..13946)
                     /locus_tag="PLF48_00065"
     CDS             complement(12747..13946)
                     /locus_tag="PLF48_00065"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF47741.1"
                     /translation="MINKKEHLVVVDGVKGFCCLLVMLAHYRFSKFNFPSVLFYVALH
                     AFFLFSAFLISRNLMVDKEKLKNFGKYFKLFYIKRTLRIFPIYFLYIFFIAFVTFLFW
                     WFGTVPQNLANEWKHYGWMHFAFVANYRDIYAYFMGYDDYIKTAPVMVHLWSVSIEEQ
                     FYIFIVFAIFFLNKKILRIMTIVAIFIFPILRVIGYNWLLAHSKDATLSTLAVVHASI
                     FQFDVFFYGLIPNLFDLSKWKNPFRSLAISLTVLICWIYLSSFIASRHDGVSFMFALR
                     EDKYIYSNGGIYFVDILTNLCVLSLIMTIVYFPHKLKFLTKKFWLVLGLPSYSIYIFQ
                     FVFLIIFLILTGLASKYIPMPIAQIIGCVFYIGSTVWMGTLLFKYVEVPMFKLKDRII
                     KKSEKLA"
     gene            complement(13948..15108)
                     /locus_tag="PLF48_00070"
     CDS             complement(13948..15108)
                     /locus_tag="PLF48_00070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF47742.1"
                     /translation="MRGFFCLCILFCHFHFTYLKLPYIVGYLLLHSFFVMSSYLITVN
                     LLYAKNKHDSFKKFFIEFYTKRVLRIFPTYFLYFFIILFIGIITAHSPLKQKLGITYE
                     IKYFGWMVVSFTYNFKDFWAWFQGLNYKASYAFPHLWSISFEEQFYLIIPFLIFMFSK
                     KWLIRISIFMMIAFAIIRIIGVDYMLSIINDPYLPSFIFIKSTFFQFDTFFYGVLLAV
                     LNPDLKKRDIRIFYVLLIVFVFSCLGNAYYLTLYNKKSIVDNLYSYIFPVEGFHRYYY
                     HVLINMICFYLVFIAVKKQSYFKLFTIPFFISVGKYTYGVYVYQYLFIFPIAFYLVPY
                     LKSLLGYDLIVEILATCLIIFLLLRFSNFIYTKYELYCINKKDLILQKLKIK"
     gene            complement(15207..17258)
                     /locus_tag="PLF48_00075"
     CDS             complement(15207..17258)
                     /locus_tag="PLF48_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF47743.1"
                     /translation="MKTTMKTIMLCWVLFSGCLFTHAQYAPTYMTAYDPNFVAVNSAN
                     FSPYGTTGYNNTQKHLATLTNLTLKIQNNLMSFYGDSRNYTPTTDTNVLLNTPAYKYD
                     RIVKSNGIIARKVMYAKKHVTGNPDPFVRMVVVRPDDNVVRPCIMITNGANWMIDFAK
                     PGGYAVWAIIADLAIRGYCVVYYESLSNDLAGLAYILPNQYPCNYYGQGDDVIRKNAY
                     ANFQYAVAAYNYILTDASLPATQRIINANTNDISAFGISMGASTAFMLAYSRAGINYT
                     THNYNLFYTGTSTHTCSNTQLSNNNYTKLCLNANPTSSINIKSLILGNSYVPADQDYF
                     TTTPKSVPTLFLWGNEDDVRINGPTSLPVNYFLPPDPASSALLLPFNSVRTTMKSLGI
                     LSRLFVNCNGGHLFFKNFATGNVTPLSCLVTNTLTDKLSMLTTSNSRDDILTLGNTIQ
                     TTDPSYPGNTIFLNCIDPVNTKYDNQQKYYNYSVDGASFRLFGTQMMYAFSGIIHSNT
                     ILTNFLKDIRTNTNNTPTLPPNSTPNFVKTNTDFSVNPFYIGTPPNQICFWYRVSSAN
                     MDGSIVPSTSCMNSGNTYTRMASPGIEEQKSEVDKSFSLYPNPSDNLLNVELYSETEG
                     HVKVEIYNSTGAIVMTMERSLQLGTNKLELNTKGLPAGMYFVRSQFGGNAIVKTINIS
                     H"
     gene            complement(17262..18098)
                     /locus_tag="PLF48_00080"
     CDS             complement(17262..18098)
                     /locus_tag="PLF48_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47744.1"
                     /translation="MSILRQYISRFIIASCFVLFSCSRIDNTKPENNKIRQSNWVVED
                     EVNGRLSSNGNIIESTNFYYNNDGTLNRLTVYDDTTYDATLIKDVFFDYTPTKVIVHG
                     YHITGGYFRVDFNFNANKQVVSILDTNGFGLRCYYSYRNLIDSFTLITDTSYAVNFQF
                     QYDINNNLIKYDIAQANHIPIGRIYMEYGTENLDTEFDLKFYSSGIKVIYTGGVNIIP
                     LLGLNIGIPNMNNLLDRKEINLLNGDTVNSYKYGYFYNNNKLTKRNIQNNTDTLFYQF
                     KY"
     gene            18204..19622
                     /locus_tag="PLF48_00085"
     CDS             18204..19622
                     /locus_tag="PLF48_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47745.1"
                     /translation="MNEAKLYYTIKSLETNEISRLKKSLNDKFVANNSASIKLITAII
                     LEKHLTAQDWNKQFIHTLIYPNLKYNDLRIRHLLSNSCKILEHFIISLKTNENKILLN
                     EQLADFYLSTGSIKYAEQYIHESILSIIHKPKNSSAYQRLFELESKLYDIQLKNSRKT
                     DNHILNMANNLSIYLIIETLKTACYIASNNVIIDEKTDFPVIKPIYQLAQEEIYYDIP
                     QIKIYATLYRLLLNWTPKEFKKILPFIKKNEFLFKNDELYNIYKLLLNFCIKEANTNS
                     HEFTKITFELYLYAIGKKYLLSYGQLNRFTLSNVIAIGLKLKDIKRAEDFLKANYRFI
                     DKNFKLMTYNYNMAKILYAKNENQKSLKLLLSTEFKDALWYLNAKFITLKIFLDMDDM
                     VRFNQYLKAFKMYIIRKQHIGYHKQYFSGVANAFKILLKIRKNSLKYQSYSFDLETPD
                     RVWFQNKAMEYVNQIKTKMQHP"
     gene            complement(19619..20449)
                     /gene="tatC"
                     /locus_tag="PLF48_00090"
     CDS             complement(19619..20449)
                     /gene="tatC"
                     /locus_tag="PLF48_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNF47746.1"
                     /translation="MGKLVKNNVKAEMGFFDHIEELRWHIIRSLIAIATASLFMFSNK
                     KFLFDYLLFGPTRPDFPANRFFCWLAQQSPYFFSGLCMQGKVHKFINTDLAGQFYLHF
                     EMAAISGAIIASPYVIWEIWRFIKPALYEQEIKKTTGVLFFVIILFLLGCMFGYFIIA
                     PFSVNFFLNYEAVGQAENLISVDNYISNLISMVLPTGIMFELPVVVYFLTRIGLITPA
                     FMRNTRKFAYIIILGAIAVITPQGDMFSLVLISTPIFILYQVSIGISQRVYNKIVRED
                     "
     gene            complement(20711..22066)
                     /locus_tag="PLF48_00095"
     CDS             complement(20711..22066)
                     /locus_tag="PLF48_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF47747.1"
                     /translation="MPKVLIIDDERAIRRTLKDILEYEKYEIDEAEDGEAGLSKIEKG
                     NYDVIILDIKMPKKDGIEVLTEMQQKGLDTPVIVLSGHGNLETAVEAVKKGAFDYIPK
                     PPDLNRLLITLRNAMDKVSLVSETKILKKKIAKSKDMVGISSPITKIKDTIAKVAPTE
                     ARVLITGDNGSGKELVARWLHEQSNRSGGPLVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRVGKFEQANGGTLFLDEIGDMSLSAQAKVLRALQENKITKVGGDKEEKVDVRVV
                     AATNKDLLKEVEEGKFRLDLYHRLSVILIHVPSLNERRDDIPVLVDHFLQEICGEYGI
                     PVKKINNDALETLQARNWTGNIRELRNVVERLIIMSDKTITKQDVLDYSGPSGKAESK
                     GIDYDKFANLQQIQDYTERSFIEHKLNKNEWNTSKTADDIGISKKQLEEKLENLGLKK
                     R"
     gene            complement(22154..23083)
                     /gene="rsgA"
                     /locus_tag="PLF48_00100"
     CDS             complement(22154..23083)
                     /gene="rsgA"
                     /locus_tag="PLF48_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNF47748.1"
                     /translation="MTGRVIQTTGSWYQVLAEGKVITCRLKGKFKLSDIDTTNPIAVG
                     DFVVLEKTENGEFVIAEILPRENYIIRQSPRQKHQRHILASNINQAILVATVSKPRTS
                     LGFIDRFICSAASFHIPVVIIVNKVDLHTEKDNKQLEIMQQIYPEIGIPVLALSAQNP
                     NDILQFHDLVQDKTSLIAGHSGVGKSTLLNALNPDLNLKTGKISEKWNKGMHTTTFAT
                     MFEVFDNSYVIDTPGIKEFATLHIEPEEVSGYFSEIKKYAESCQYNNCMHVQEPNCAV
                     IKAVEDGKIALTRYENYLNILHDINEVNYWERK"
     gene            complement(23099..24523)
                     /locus_tag="PLF48_00105"
     CDS             complement(23099..24523)
                     /locus_tag="PLF48_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNF47749.1"
                     /translation="MHETALKALFQSSFNHEADVINKLPQSGSDRIYYRLSNKEHRVV
                     GTYSADIKENETFIYMAVHFHSKDVAVPQVFAASQDHHYYLQEDLGDVSLYSVIQQEG
                     MSERVISLFKKSLERLAYLQIIGAEDFDFNKCYPINAFDKSSMFWDLNSFKYYFARVA
                     RVHFNETELNKDFHKLTDYLLNERHLYFMFRDCQSRNIMIKNEEPYFIDFQGGRRGAL
                     QYDCASLLWQAGAKIPMDLRQELFDFYVNEVKKHVAIDEADFRERYQGFVLIRLLQVL
                     GAYGFRGLIERRPHFIQSIIPALENLKIFIPHIKDLPELKSVLQQLTESDIFKYIQYD
                     GSHKRLIVEVNSFSFKRGIPKEEHGNGGGFVFDCRGLHNPGRYEPYKQLTGKDQPVIE
                     FLETQSNVKSFLDTIWQTIDLTIQNYLERDFDHLQINFGCTGGQHRSVYCAEQTAKHI
                     RDKYKVKVELKHIEREIADQAKND"
     gene            24696..25439
                     /gene="cobA"
                     /locus_tag="PLF48_00110"
     CDS             24696..25439
                     /gene="cobA"
                     /locus_tag="PLF48_00110"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNF47750.1"
                     /translation="MTSRKLILVGAGPGDPELISLKGIRALQQANVVMYDALVSEQLL
                     DYAPNAIKIFCGKRAGSHYMKQDEINQLIVEQAETHGTVVRLKGGDPFVFGRGHEEIA
                     YAEKHGIFTEVIPGISSATALSSQHKIPLTHRGITESVWITTGTTSCGKLSDDIELAA
                     QSNATVVILMGIGKIDEIITIFTKYKPQDFPIAIIQNGTLATEKIISGQLNTISEWVK
                     QEQISAPAVIIIGKVTALNNLSFPQTENE"
     gene            25441..26022
                     /locus_tag="PLF48_00115"
     CDS             25441..26022
                     /locus_tag="PLF48_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNF47751.1"
                     /translation="MNTLYPIFLNTANIRILLVGGGHTAKEKLHFLLKNSPDAQITLV
                     AKAVIADILPDENLAKNFVTILQREFEPADLHGIHIVIAATDNPVLHQEIYQLCKTKN
                     ILINVADTPALCDFYLGSIVTKGDIKIAISTNGKSPTLAKRLREYFEDILPDEIATLA
                     DNLNSYRNEIKGDLQDKIRKLNELTRSFLKPKE"
     gene            26035..26724
                     /locus_tag="PLF48_00120"
     CDS             26035..26724
                     /locus_tag="PLF48_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNF47752.1"
                     /translation="MEKIQLPLLSRTMDAYFYAASGEEKRPGILFLPDLTGIQKTTHK
                     SAKILSEEGNFNVMIPDLYSGGVNMSKYCMQFFFNGLVRNNEPTGNAPLIEFFEILDQ
                     FKSFQEVDANAIGVVGQCLTGGFVLHAAIRPEVKAPVVFHHSFGQKGSGIPKSCSAMV
                     QNKVQGHFVYVDGFCPVWRVRKLENELGENLETHWYNLPHGIPHLFFNNEQGKKAFER
                     MCTFFEDQLNS"
     gene            26727..26963
                     /gene="yidD"
                     /locus_tag="PLF48_00125"
     CDS             26727..26963
                     /gene="yidD"
                     /locus_tag="PLF48_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNF47753.1"
                     /translation="MKNAFKNILIFPFILLIKVYQIGLSPMLGPNKCRYQPTCSNYAL
                     EALKKHGLFKGGYLATKRILSCHPWGGSGYDPVP"
     gene            complement(26976..28289)
                     /locus_tag="PLF48_00130"
     CDS             complement(26976..28289)
                     /locus_tag="PLF48_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF47754.1"
                     /translation="MTRFKKITALLYLLSLIISASAQDLLTPEDAVAYAVKNNYDIIL
                     AKNEADILRINNNKGAAGMLPKINVTTGDVFNLSNIHQELSTGTVTNKNWVPVNSFNA
                     GLNLNWTAFDGLKMFATKDRLQALQVLGELQLKEQIQNTIAQTLAAYFEIVRQKQQLK
                     AMYEWVKLYEERVNLSQKKLDVGYSDKTPLLQAQVDLITQKAAIVKQDFVLQQSKAVL
                     NNLLSRDAETGFDVIDTFAMNYQADLQQLKDTTLNNFSILSAQKNIDIAKLQHKEITA
                     QKLPIINFSTGYNFNQNNSKAGLQLVNRSYGPTVGINAVIPIYNGGIIKKQLEASAVN
                     IAVKELVVSQLKNDLQQKVYNAYKNYEYAKTVLKLNEDAVKTADENVQLSMQRFRLNQ
                     TTTLEVKTAQSSYEMALFNVILARYTAKLAEIELRRLTDNFVQIK"
     gene            complement(28316..31405)
                     /locus_tag="PLF48_00135"
     CDS             complement(28316..31405)
                     /locus_tag="PLF48_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF47755.1"
                     /translation="MGLPSVSLRRPVFAIVLNIMIILFGVIGYTFLGVREYPSIDPPV
                     ITVRTSYTGANADIIESQITEPLENAINGVQGIRTISSASNQGTSNITVEFDLSADLE
                     TAANDVRDKVSQAMRQLPQDIDAPPVVTKADANSDAIVSMTVQSDTRNILELNDYAVN
                     VLQQRLQTIPGVSTIQIWGQKNYAMRLWFDPQKLSAYQLTPIDVQNAVNKSNVELPSG
                     KISGKNTELNVNALGRLNTELEYNNLILKDVGGQTVRLKDVGYAQLGTDNYETSLREK
                     GVGMVALALVPQPGANYVEIADEFYRRLDKIKKEVPADIQLNIALDNTVFVKNSISEV
                     KETLLISIALVVLIIYLFFRDWIIALRPLLDIPVSLIGAFFIMYIFGFTINVLTLLAI
                     VLATGLVVDDGIVVTENIFKKIEKGMPRMQAAREGSEEIFFAVVSTSITLAIVFLPVI
                     FMQGFVGKLFKEFGIVVSGAVLISAFVSLTLTPVLNVYLGKKVHTHSWFYDKTEPLFK
                     GLDDGYKEWLTSFMKVRGIALVIVLISFGLIYFIGKNLQTELAPLEDRGSLRVQVTAP
                     EGTAYPAMQNYVDNLSQFVTDSVPEEKVVLSVTSPGFAGSGAANSAFMRIRLKDKIER
                     TRSQSDIAQSLNRNFAKFNFGKAFVIEEQTISVGSARMGMPVQFVLQNFDFDKIQQAV
                     PRFMEEVSNSPVFVGADVNLKFNKPEVDITIDKEKAMSLGLTTQDIAQTLQFALSGKR
                     FGYFLRNGKQYQILGQSLYAERNKPDDLKSIFVRNNAGKLISLDNVISISTYSKPPQL
                     YHYNRYKSATISSGLASGKTVGDGIQEMEKIAKKVLDASFTTSLTGASRDFAESSSNT
                     MFALILALVLIYLVLAAQFESFVDPFIIMITVPLALAGALLSLWIFGKTLNIFSQIGM
                     IMLIGLVTKNGILIVEFANHNRRLGMSKIDAAIYAASMRLRPILMTSLAMALGALPIA
                     MALGAGSTSRTSLGIVIIGGIMFSLILTLFVVPALYVFFSRTKSEQKEEALQANTIE"
     gene            complement(31415..32509)
                     /locus_tag="PLF48_00140"
     CDS             complement(31415..32509)
                     /locus_tag="PLF48_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF47756.1"
                     /translation="MTKGKIIFFSCILAIIAILAYVKHTKNKEAAAKEMPKGGKDKGG
                     SKTVFASGFVVEYAKLTNELSANGTVLAAEEVSLQPEVSGRVVYLNIKEGAFVGKGTL
                     LMKINDADLKAQVQKSKAQLKIAETNLKRLGELLKINGVSQAEYDVADNLVNNINADI
                     EILKVQIAKTELRAPFSGKLGLRNISLGAYVSPQTVVATLQNLQQLKMDITMPEQYAM
                     SIHQGDVMECNVAGILKPFLAKVYAFEPQIDEATRNLKVRAAILNPDAQLLPGTYVKV
                     MLKLKDIAQAIMIPSNCIIPDDRATKVVVADSSKAKFVNVEAGLRTASEVQILSGLKP
                     GDTLLTTGLLQVKPGMPVKITKTTQKSTIQ"
     gene            complement(32506..33123)
                     /locus_tag="PLF48_00145"
     CDS             complement(32506..33123)
                     /locus_tag="PLF48_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF47757.1"
                     /translation="MQAKRRQEDKRNSLKRMILREAKEIAASEGWQNVTIRKICDKIH
                     YTAPVIYQHFENKEMILCAIRIEAMTSMYEIFEEIDKKYEKPDKRLLKYGLAWWHFAQ
                     TNPELYQVMFNLQGALCIDIGTDKPTYNTVIDFYKSAFSELNRDAKKSEKLMIEFCDN
                     IIAIIHGFISLQMVGKIRSGKEHAEDTFKNALKRFIHSIKISKQP"
     gene            33682..34425
                     /locus_tag="PLF48_00150"
     CDS             33682..34425
                     /locus_tag="PLF48_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNF47758.1"
                     /translation="MQVSIFVWIWIAIAVLIFPIQLFITAPYGRHTKKSWGPMISNKV
                     GWFMMEAWAPVWFVIVYLLHPSYQFYNLLFASLYLLHYINRGFIYPFRTNTKGKQMPL
                     VIAMSAMLFNSVNAGTIAYYLSRVSVYPDNYWQSWHFIAGVFLFILGFVINLKADDML
                     IHLRKPDETGYKIPRGFLFEYISCPNHFGEIIEWLGYFLMSWNLASLSFFIWTFSNLF
                     PRALKHHQWYKAHFEDYPSERKAVLPGVV"
     gene            complement(34430..35008)
                     /locus_tag="PLF48_00155"
     CDS             complement(34430..35008)
                     /locus_tag="PLF48_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47759.1"
                     /translation="MRKLIYIASSVVVLIIASQFTACIKCGPFDVKTFKVTEFGVTSY
                     ATTNLSFDTTKLDTATILNAMYFKINPVHYYYVSNKSNPYQGLISTAFACSPPSNYSV
                     DKIRQIEIYPSQDISTTITTETDLSSYIQLSYPISINEDYISNISFFPSRLFTDYTNI
                     EYKPLRFLFKLHLSDGRILSSTSNHIYFPTNL"
     gene            complement(35034..36836)
                     /locus_tag="PLF48_00160"
     CDS             complement(35034..36836)
                     /locus_tag="PLF48_00160"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNF47760.1"
                     /translation="MAYSSLQQCIDDLEKSGQLIRIKEEVDPYLEMAAIHLRIHEAKG
                     KAILYENIKGCKYKAVSNLFGTTARSQFIFRDTLQDVQELIELKTNPLNALKKPLKAL
                     RLAFKAINALPKKVKSHPFEKIKISDLPQIQCWENDGGAFVTLPIVYSEDADSPGVMQ
                     SNLGMYRIQLSGNEYVQDKEVGLHYQLHRGIGVHQTKWNKLGKPMKVSVFVGGPPSHT
                     FAAVMPLPEGISELTFAGILGGRRFRYMYDEEGFCLSTDADFVITGEVYPNDNKPEGP
                     FGDHLGYYSLKHDFPVMRVKNVYAKKNAVWSFTVVGRPPQEDTAFGNMIHEMTGDAIA
                     KEIPGVHEVHAVDAAGVHPLLLAIGSERYTPYYTEKRPQEILTIANHILGTGQLSLAK
                     FLFIADKSDDETLTTHDEKKFFQHVLERIDLSRDIHFQTKTTIDTLDYSGDGLNTGSK
                     VIIAACGEKKRDLLDELPNDFLLPNLFKNPKLVSKGILAVELTVDSRQSTVGTIINQN
                     RGQWTEDSGLPLIVVCDDADFLAADFNNFLWVTFTRCNPSHDIHGINERFENKHWSCD
                     ALIFDARIKPHHAPVLEKNAEVEERVDEVLKNII"
     gene            36981..37712
                     /locus_tag="PLF48_00165"
     CDS             36981..37712
                     /locus_tag="PLF48_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF47761.1"
                     /translation="MKWAFFSWLILSNIISESTSILNTEVYVPMNSSDAGIIYAQDYY
                     TYYFAYKNIGNEPLIICHVSGSSGCVCPTYSKEPIMPGNWDTLRVTYSAQYTGRFHKT
                     VTVCWNTKEALSIFNISGEVRTLPDKLFIYDSVHHKYAIFDSIDYRVIKFMGQNCYTI
                     TNMDSISHQFSIDKYHGLEYVNSGWIFPSGYTCYFHQNEHILYSSELRSGKSINLHFI
                     RKEEFIHEIELLMDGTYYIRIKIDY"
     gene            37745..38806
                     /locus_tag="PLF48_00170"
     CDS             37745..38806
                     /locus_tag="PLF48_00170"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNF47762.1"
                     /translation="MYSLIRFFLFKMDAEKAHHLTLKIMAFAIKILGIRMLLNKLFAL
                     DDKRLERTVFGITFKNPVGLAAGLDKNAQCIDALSALGFGHIEIGTVTPLAQPGNDQP
                     RLFRIIPDEAIINRMGFNNEGIQTVGSNIISSKQFKQKNKEGNSRTIIGGNIGKNKIT
                     PNENAVDDYLKCFEALYEYVDYFVVNVSSPNTPDLRALQEKEPLKKILFSLQEKNNQQ
                     VKRKPVLLKIAPDLSNEQLDDILEVVADTKTDGIIATNTTLSRTGLSISADEITAIGA
                     GGLSGKPLTQKSTAIIRYLAKKTKGELPIIAVGGIMTAQDALDKLEAGACLVQLYSGF
                     IYEGPRLIKQINKKILLSK"
     gene            complement(38829..43697)
                     /locus_tag="PLF48_00175"
     CDS             complement(38829..43697)
                     /locus_tag="PLF48_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47763.1"
                     /translation="MRNSFILTVLILLIRFSAFAQYPKEFPADNAGYAKSFSDFIKAN
                     CSRDDCKELAEKFPKVITAGKASTYFPKIKNITQALLMRKAPAYPVMFNFATMLGNLE
                     TAKASTAAIDKNFDILQAIADRSKGGNLKEFDTYVNFLNNLYIKNTLYASNTNNWYAS
                     GDYSVDIKDDKPVYSFLSTDLMGISTADTINIKGESGKYYPLENLWIGNKGNISLQRA
                     GFDPANNFLNFGNHQINFSKTDIVIDSAKFTFKPVLNEVLLGTYLDKLLQAKSQERVY
                     PKFTSYKKDVNFNTLSKEIGLVGGFQLEGTNIYVVGSDTSQPVVTLKNKENKEVVKAT
                     CKRIAIKDFKDLSLTDASLAILLKDNTITHNYLNINYSSKTKNLMAYRDMKPLSKQPF
                     TSDYHQMFIYADELSWNIDSLTMKFGMATLSGEKPAIFESYNYYQQNIENKYKGSDEQ
                     GPISRIYAFYAVANERKLDAQSVAFDISPNAPYNAVQPTFVKLMEDGYIKYDASTRTI
                     EVKDKLINQALSAKGKQDYDFIKFASFKKYGNAKLDIKSNILEVYGVDEINMSSKSGV
                     KFIPSTDTVKIGKNRTMTMGGRIIAGKFDFLAKKVDFDYENYAFNMNKIDSMVVYVPN
                     DKPNEKGQYVLSPSRSPIQNISGTLHIAEPENKSGTNNNLKYPFFSSGDTSKVTYDRG
                     ANGDKYNKDKFYYQIYPFEIDSLNDVLTENLDFKGRLISGGIFNPIESGLTLQDDKSF
                     GLQIETPKGGYDLFGNKGKYFSDLKLNADGLSGKGLFEFGSIKMYSDTSFFFMDSVYA
                     KLDSIRITQDKKANKPEGKINASSFVWNQKIDSLVVSPAKDEKFTFYDNVVDLNGKMI
                     LYKKDLLGKGLLSIGKTKLESDEIAFGATQFSAKNAKLNLVTEEGNSLLASDAVNALY
                     DLDKKIADVELIKNDTIPLASFKYVSNPKNLHFDLAKNMLTLNAASTSAKFFLLSTDP
                     TKDSLKFVTNTAELNLNDNSIHFAGINELLLADSKVIPDKGEIFIEQDGTVRKLKNAI
                     VIFNRDSSYHTVKDAEINVVGRNDFSGTGNYYYKNGASSTKVSMAEIGVNNPYKGQVI
                     PEGKKGKKTLSDRDYSKVFTYANTIIEEAANFKLDNKVFYKGNFNFDSKHKNIFLDGD
                     IKIEIAGTSSDWIPNKQELDPNKPAINLESVLKDNNNIFVGLLLDKTNIEFYQAILQD
                     KHSPGDAAIMNVRSTMSNSKTEPNTMLFGDESALTKYYSHASCIKYNETSKQMDANGE
                     IGLGLDLYPCAVTTAGSFTFSPSKPTELTLAADMALKFKMNPVISNQLINAYLNAETD
                     VTFANSYKRNKTVQRTMSVLTKDSLEANQLLAGMYTTDSFFIPKSFDYNLLLSGTQFF
                     WDAQDASFKSVGNVSLAIFGNDIIKRTYNAYVEIGYAYESDFVNIYLESKSGNWLFMK
                     VKKGQMGIASSAPEVMNTLAALKSSDKVFRENKLTIFEFMPADLMMRDNFVIRMEDFK
                     ERFKSKIPVSAPLKTPSAPVQQEQPNNIEQPTPVTPSDSSEVIKNNTLTPEQPTPPKE
                     QKDEFKQKPKKAFPLSQPAPTPKKEEQPESETPATEVNPEAVPETPAPAQDSTTTVPT
                     PKKTTKSKTKPK"
     gene            complement(43710..44534)
                     /gene="prmA"
                     /locus_tag="PLF48_00180"
     CDS             complement(43710..44534)
                     /gene="prmA"
                     /locus_tag="PLF48_00180"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNF47764.1"
                     /translation="MKNYIAKIELPQNIDAEEFEGVFYTLDCEGFEAKEQEVDVYFNE
                     SQQAEMESFLKDIAETYSVKYTIELLENKNWNEQWEAGFQPVVINDFVAVRATFHPAI
                     QDVEHEIIIDPKMSFGTGHHATTMQMMQNMRLIDFKEKTVLDLGSGTGILAILAEKLG
                     ACSVVAVDNDEWCFTNAEENCKLNHTQKTKNILGGIEKVDEQKFDIILANIHRNYLVE
                     NMQNSAELLKSGGYLFCSGFYDTDAKFVLDAALECNLYTHYLTTLNQWDCIIFGKQ"
     gene            complement(44647..45414)
                     /gene="tpiA"
                     /locus_tag="PLF48_00185"
     CDS             complement(44647..45414)
                     /gene="tpiA"
                     /locus_tag="PLF48_00185"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNF47765.1"
                     /translation="MRKKIIAGNWKMNTEKASAEALVDTITENYNTFGLHEKKQVVLA
                     PPFPFLNIALDKSADYPYLYVAAQNCSEHEKGAFTGEVSAVMLASVCVKYVLIGHSER
                     RQYFNEGDLLLLEKIKQAKRNEIIPIFCCGEPLGIRMANEHFKFIKAQLDNCIFQLNE
                     EEIKNLVIAYEPIWAIGTGQTATPLQAQEVHAFIREELALRYSKEVADSISILYGGSV
                     NGKNAMDLFGCADIDGGLVGGASLKADEFAEIICSMK"
     gene            complement(45424..46608)
                     /locus_tag="PLF48_00190"
     CDS             complement(45424..46608)
                     /locus_tag="PLF48_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNF47766.1"
                     /translation="MPKTHFILFDDEQRDHLLPLSATRAIADMRIGILTIKEKWEKYL
                     HAPVEILTVPYLQPKYTCLLKENTIYINARFLPDATLLNDIKNLNEHTKLQSEDGQVI
                     AIHSVAKLNIGEVLNYNEVHCQIIKLSNYQIKYPWHIFQHNGNEIRNDVALLGLKPNP
                     EKLSATNQLLGSEIYVEDGVKCEFAILNSTDGPIYLGNDAEVMEGCTIRAPFALCEHA
                     TLKMQAKIYGDTTIGPYCKIGGEISNSVISGYTNKAHDGFLGNSVLGEWCNLGADTNN
                     SNLKNNYGKVKAYNYPAKDFINTGLQFCGLIMGDHSKAAINTQFNTGTVTGVFANVFS
                     GDFPPKYIPDFAWGENAIFQLDKALEVAQRVMERRGLLLSDEDKAVIIYLYEQRTVKA
                     LT"
     gene            complement(46675..46926)
                     /locus_tag="PLF48_00195"
     CDS             complement(46675..46926)
                     /locus_tag="PLF48_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNF47767.1"
                     /translation="MKADIHPKEYRLVVFKDFSCDHTFLAKSCAPSKETIKWEDGNEY
                     PLVKLEISNMSHPYYTGKMKFIDTAGRIDKFNKKYAKKK"
BASE COUNT        14965 a         8749 c         8076 g        15270 t
ORIGIN      
        1 ggcttctgca ccgaaatggg gcaaaatgaa tgtgggacaa atgctggcac acagcaatgt
       61 aacctatgag tatgccttag atgagcgcga cgacaaaccc aattttttgg tgaaaatgat
      121 gcttaaaaac tttgtaaaaa acaaagtggt ttccgaaact ccttatacgg aaagcatgcc
      181 tacaggtccg gcatttgtaa ttaaagaaag taaagatttc gacaaggaaa aaacacgatt
      241 aatcaactat atcaacaaag tgaaaagctt aggtgctgct cattacgaaa gtaaagaatc
      301 atcttctttc ggggtgttaa ctgccgccga atggaacaat atgatgtaca aacacatcga
      361 ccatcatctc aaacaatttg gcgtataagt atcttgctga attttcctta tcaaactgag
      421 ttcgggttaa gaaaaataaa gtgataagat atttgatttc tcattttcct ccaacttaac
      481 aaggaagagg aataaaagga ggtgcttata aagaaactaa atcaactatt ataaaaaatc
      541 gaaacgatta tcccaaactc aggttatcag attttattca gcgcatctac ataggctttg
      601 gctgtggcat aaatgacatc cgtatcgtta ccaaatccat agtaaaaaac gccgtcttta
      661 gaaatttgga tatgcacttt tcccgtatca tctgaacctc ccgtaatggc ttgcactaaa
      721 tattcttcta accgcactga atgttttact attttatcta ttgcttttaa ggtggcatct
      781 accggtccac agccgtcagc aatttctacc gtgcgtttac cttctttcat cagtgccacg
      841 gtggccgtag cctgatggcg tttgccgcaa tgcacttcca aataatcaat ttcatatatt
      901 ttaccttcgc gactttgtcc catcaactcc agcaaatctt cgtcataaat ttctttgcga
      961 ttgtctgcca tatccaagaa acgggcgtaa gtttcatcta actgtgcctg gtctaaatta
     1021 aacccgatgt tttccatgcg atgtttcaaa gcagccctac cgcttcgggc agtcaataca
     1081 attaaagatt cgggaatacc gacatcatgc gggtctatga tttcataatt ttccctgttt
     1141 ttcaacactc catcctgatg tatgcctgat gaatgtgaaa atgcatttct tccaacaata
     1201 gctttattcg gttgcacagg catgcgcatc agtcttgtaa ttaatctcga agtaggatat
     1261 atttttttga tgttaatgcc gcattctaaa tctaaattat ttttatgcga cttgagaatc
     1321 atcgctattt cttcgagtga agtattgccg gcacgttcac cgattccatt catcgttact
     1381 tccacctggc gggcaccatt ctctatagct gccacagagt tggctgttgc caaacccaaa
     1441 tcattatgac agtgtgcaga taaaatcgct ttatcaatat tcggcacatt attcatcaaa
     1501 tacttaattt ttgcaccgta ttgttcgggc aaacaataac ctgtcgtatc gggaatatta
     1561 actaccgtag caccattcgc tataaccgtt tctaccattc ttgccaaaaa ttccaaatcg
     1621 gcacgtcctg catcttcaca aaaaaactca acatcatcca cgtaattacg ggcatacttt
     1681 gctgcccagg ctgctttttc cagtatattt tcccgtgtcg tttttagctt atttttgata
     1741 tgaatatcag atgaaccaat gcccgtatgt attcgggctc gcttagcatg tttcagcgcc
     1801 tgtgccgcac aatcaatatc tgcagctacc gcacgggtga gtgcacaaat ggtaggcttg
     1861 gaaatatttt tggtaatctc taccacagaa ttaaaatcgc cgggtgatga aatcgggaat
     1921 ccggcttcca gcacatctac gcctaattct tcgagtgcca atgccacttc aattttttcg
     1981 atggtattca actggcaccc cggaacttgt tcgccatcac gtaatgtggt atcaaaaata
     2041 aaaatctgct tactcataaa attaaaatga aataaagaaa tgcttgcatt taaaattaaa
     2101 aatttatgca agtcatttaa ttactcttac attaagaata gattatctgt gtatgtcggt
     2161 aaagtattat aactgttgca aatccaccag ttttttatat tcacccatca atgctgttaa
     2221 ttcggcatgt gtgccttgtt ctattatttt tccttgttta agtaccacga tttcatcggc
     2281 aaataatacg gtagaaaggc gatgcgcgat gatgatggct gttttgtttt ccagtaaatg
     2341 ctgcaaggca tcctgcacca atttttcagc attgctgtct aagtgcgagg tggcctcatc
     2401 cagaatcaga atcggagcat tcttataaat agctctggcg atcgtgagtc gttgctgttc
     2461 gcctccgctt aatttagatc cattatcacc gatattggtc tgatatttat ccggaagtct
     2521 ttcaataaaa tcatctgcaa atgcttgttt agcagcttgt cttattgttg tttccgtacc
     2581 ggtatgcccc agcgcaatat tgttttgtac actgtcgtta aagagtgtag tatgttgtgt
     2641 gaccaaagca gtaagcccgc gcaaatcctc ttgttttatg tcttttatag gaatattatc
     2701 cactaaaatt tccccgctgc tgacatcaaa gaagcggagt aaaagttgaa tcaaagtaga
     2761 ttttcccgaa cctgaaacgc cgaccaaagc aatttttttt cctttctgta tctttaaatt
     2821 tatacccgat aaaacatctt cattttcata acggaaagaa acatttttaa acactatttg
     2881 ttcgtcaaaa tttgtttttg aaatagcctt cggaacatct tttatcttgt tttcagtgtg
     2941 caaaacggtc tctattctac tgagtgatgc aaggcctttt tgaataaaat aaaaggcatt
     3001 ggaaaaagct ttagccggtt gtatgatttg agaaaaaata acgataaacg caataaatgc
     3061 ctctgcacta agcgaggaag tatgattaat caccatttta ccgcccacgt ataacacgat
     3121 tacaacgacg ccgataccta aaaactccga caacggtgta gataaatccc gcctgcgtat
     3181 catagatgtt gtcagttgat agtgatgttg tgtaatttta ttaaaccggt ttaaaaacaa
     3241 cttttctgcc gtgaaagatt taatgatacg caagcccgaa atcgtttctt caacctgcgc
     3301 attaatatcg ccttgcagct gttgcgcttt tgcagaagct tgctttaatg atttgccgat
     3361 tttgccgata atatatcctg aaacgggcaa tataatcagc acgaacaagg ttaatttagg
     3421 gctcattaac agcatggtta ctaacgtggc aataatggta acaggctctt taaatgccac
     3481 ttctataaaa tgcagaatgc cgtattccac ttcttgtacg tcattggtaa gtcttgtcat
     3541 gatatcgcct ttgcgtttat ccgaaaaata caacacatct gcaaagagaa ttttttcgta
     3601 cagttgcttg cggatatttg tagccacacc tgtgcgaaca ggagccagaa aatgtaccgc
     3661 cagatagcga aatatatttt ttaataaaaa aatggcaaca gtaagcaagc aaataaacaa
     3721 cagcgctttt gttttgccgt cagcagtatc ttgaatataa tacagcaagc ggacattcag
     3781 gctataattt aaaaatgctt tgaagttgta caagctaaag ctaacgggag tttggcttac
     3841 ttgtatctta tctgaattaa aaatgagatt taagaacggg attagcatta agaacgagaa
     3901 aatggaaaac aacgtggaga gcagattaaa caaaatgttt aatgcgatat tagataaata
     3961 ggggcggcaa tatttgagta tttgtttcaa aaaaaatctg attgtaaagt gtaaagatat
     4021 taattacatt tgtttttatg gaaacgatac gacaaagaca aattgccaag acgattcagc
     4081 ttgctctttc tgaggtcatt cagcaagact tatcctttga tttacaaggt gctttggtga
     4141 ccattgcagg tgtaaaaatg acacctgatt tattaactgc acgaatttat ctcagcattt
     4201 tcaaccacaa aaatccggat gaacttcttg aatattttga tgcacacaac agaaccatac
     4261 gtggtttgtt aggcaaaaag attcgtaata aggtacgaca aattcctgct ttggaattct
     4321 ttaaagaaga ttcattagac catgtatttg agatagagca attgcttaaa gatgtaaaag
     4381 agaaagacga ggagatagcc aaataccgca ctgacgaaga cgaatcagct gatgcttaat
     4441 ctcgaactga atattgccaa acgctatctg ttcagcaaaa aatcgacgaa tgccatcaat
     4501 atcatttcgc tggtgagtgc atttgccatg ctattcggaa caatggcttt aattttggta
     4561 ctttctgttt ttaatggctt agaagactta gtgaagtcgc tgtataatgt attttactcc
     4621 gatgtggcag tttttccggc taaaggaaaa acctttgaaa ttacggatga actgaaagcc
     4681 aaactgaaag cggtggactt tatagaagcg agctcgtttg tattgcagga aaatgcattg
     4741 cttgagtatg agggcaacca gcatatctgc accattaaag cggtggatga taattatttt
     4801 aaagtagcaa aaggctttga tacatttatt gttgacggga aaaaaatttt atacgctgac
     4861 agtataaact atattattcc cggagtaggt gtagcacaag gcttgggttt aaataatatt
     4921 atgacattga atcctgtggg catctacatg cccaagaaat cggcgagcaa ttttgcactt
     4981 ttagaaaatg catttaacag aaattatctt actccttctg ccgtatttgc cgtttcagat
     5041 gaatttgata caaagtatgc gctaacatca ctttcatttt ttcaaaacct gaccgaaaac
     5101 tatgacaagg cttcagaaat agacatccgg ctgaaaaatc caaaactcaa cgagaaagca
     5161 atccaatcta ttaaaaacaa attaggtatt ggatttgaag tgaagagcag gtatgaacaa
     5221 aacgaagtgt tgtacaagat tcttaaaagt gaaaaatggg taaccttttc cattttagta
     5281 ttcatcattc ttattgcttc ctttaatatt atcggtgcac tttctatgct ggtgttggaa
     5341 aagaaaaaag acattgccac gctcacggct ttaggtttca agcagcgcag cattctgaaa
     5401 ttgtttttat atgaaggttt gctgatgtcg ggtttaggtg ccatttgcgg gatggttctt
     5461 gctgttatcc tgtgtattct gcaacaaaaa attggtttga ttcctatgcc cggaacttcc
     5521 tttttagtca aatattatcc ggttaaaatg aatctttcag attttgccgt agttagtttc
     5581 gtcattatag caataagcct cattgctgct tggtttcctg caaaaaaagc ggcaaaatcc
     5641 gtagaaaatg aatatcttac ttacttgtaa tcccatataa aaatgaaatc aaaaagcttc
     5701 tttatactac ttctgttttg tatcaatctt ggctttgggc aaatgcgttc aaaattagat
     5761 tctgccataa atactacagg ttatttgtgt aaatatgaag tattgcaaac agccaatttc
     5821 aaatcgctgc gttttgatgc tgttagatat tccaacttag aaaatttttc tgtcctgagc
     5881 ggattaaaat ttatttataa aattcagtcg ggcaaagaac tgaaatccat gcatgcttat
     5941 attgatcaga aagatgtaga aggcttgctg atgacgctac aatatatgcg cactatgctg
     6001 aaaagcaaaa ccatgccggc taattatacc gaaataaaat tcactactac tgcaggcttt
     6061 caggtaatgc tttatactat cttaaatgac aagaataatt taagctggaa attttctgtg
     6121 atagccaatg tgcaaaatga taaatcttat ttagaatgca caactgatga tattgttatt
     6181 ttggaagaag cgttgtcatc attaaaaaac aaaatatgaa ataagccggc tacaataagc
     6241 taaaatgcat ttaccttttc tatttcgtaa ataaaatctg atgtacaagt aagtgcagat
     6301 gaataggtat tatcaatttt taatgctttg tagtataaag tctctccttc gcaaataatt
     6361 tgaaaatcat taagtacaac agttttcccc aatacttcta cacctttacc attgcatttt
     6421 atcgcacttt cttttatcgc ccagtagtca aaaaattgct gacccgggtt ttcatttttt
     6481 tgaatgtttc cccactcttc atccgaaaaa tacttgcgaa ataaatcaat ctggatagtg
     6541 cgatgcattt caatatctat accgattctt ttttcagcta cagccaacac tacccagttg
     6601 ccggaatggg caatattaaa atcaatatta cttgcaaagt atggacgacc ttttgtagtc
     6661 attcttacat cctgcaaagc aaaatttaaa ttcaattttt ttatggcata attcaagagc
     6721 aattttccta ctaaactatg catggcagtt tgtctgtgtt tatactccaa tattattttc
     6781 tgcataaatt ccggaagggt atgcaaggcg tttgcgaatt tttcttcact ccagcttttt
     6841 tttatttcat aaacaaaaat ctgaatctta gtcattgcct ttcaacttag aaattatgta
     6901 tgctgctgct ttttcatttt cgtacagaaa aaaatgtccg ccatcaaata tttttacttc
     6961 aatatttatt gtaaaatgtt tttgccagtt ggcagaagtt tgttcattgt ttttatgcaa
     7021 atcttcagca ccataaagga tataagcagg aaccggcagt tttggtttta gagcatacgt
     7081 atcgtgataa tcttcaagta tagaaaaatc tgctcgcaaa acgggttcta taacttccag
     7141 aatttcggga tgatgcaaga gctgtcttga tttatcatca taggtcagaa tatgttccca
     7201 aaaaacttct ttttcagata atgcgaaacg ctgcaaatcg ctataatctt cctcgctgcg
     7261 tctggatgcc aggaacaatg cacgaggtaa gggatgacct tcagcctgta aaagatgtgt
     7321 aagctcgtat gcaaagacaa ctcccatact tacgcccaca aaaaaataag gctcatttat
     7381 ttccggctct acatgtgtta acatatcgtg caaggcaaat tcaaagttgg ttaatctttt
     7441 ctcatcaaac cgatcgccgc gccctggcaa ttcaatactt ttagtcgcaa aatgagctga
     7501 cagtttttcc tctacacgct tgaaagcata acggtcgcct ccggcatgat gaagtaaaat
     7561 agctttcatc ttacaaatgt agaaaacaca cttgttattc taaagtcaaa tctgatttat
     7621 gaaacacata atattacttt cgaacgcatt tattctttta tcagcaaata agtttcgcat
     7681 tcactaaaat gcaatggctt gaaacctctt gctgtagggt tattaaaaaa cgagatccag
     7741 gttgaatcat atataatgtg tggatcgcgc agcaaagatg catttacaat gacaatatca
     7801 atttttgcag aatctgcata atgagcataa ggaataagtt ttttcttatc gaaaatcgta
     7861 tttacttcat gaaagttttc gggtaacata cctctgacaa aaggcatatt ggtaaaaatg
     7921 gtgtgtggtt tatcattgta attttgctgt agcaatcgga tcattttttt attgcagagc
     7981 ttcgcgttag ccgagtccat ttttaaaaat ggatatttgg aataagacgg acaaacacac
     8041 aaacatatca aaaagagtcc caataatatg gcttttacat ttattttctc tggaagcagt
     8101 gggttcagta tgctaataat tatgagcaca aaaaacaaca tctgcaaata aatataatgt
     8161 tcacgcggaa aaacaaaaac acatgacaat atggtgggag aaatgaatac caacaggaat
     8221 aaaatataga atatataatg cgaaaactgt gtttttattt tgtttcgagt tgttttgttt
     8281 atcaagagta aaatcaatcc gatataagaa gaaaatgcta tgagaaattt aattttattg
     8341 gaatgaaaca aaactaaagg aaaaagataa gaaccaacag agacaaaagt aaatgcaatg
     8401 taatttttaa tattaaaaag tgtattcaat ataaatatct ttggctgatt tataattaca
     8461 caaagcatag agttacaatt tccgaatgta cttttagcta aatcttccca tcctaaccat
     8521 gcatcaacgt ctaatttgtg ttgtatacgc agattaaagg cataatgctg ataaaatgca
     8581 gctactcccc gattgaaacc aaagaaatta ttggaaggga aacgaaaaaa caaatgcaga
     8641 cttgctatga taattacata aaaaattatc catcccgttg ttttcttgtt aaatgtaatt
     8701 ggttgataaa aacccaatag tattgtgata aataataata gtataaacgc caaataaaac
     8761 tcaggtcggg catatgaaca taacaaggtg accaacgtca ggaggagtaa tttgtaaaat
     8821 ttgttcttga aatacgtact taagattaat ccggacaata agagaattat acaaaactga
     8881 gaaactcttg gccacacaga aatattcgct tcactgacta aaacacagta tgagataaag
     8941 aaagccaaaa taaaattcac acccattcgc cataaaaaca acaatagcaa tataggtgga
     9001 atgatggctg ttagtctgaa attgaggtaa taaagtttaa ttgtatcagt agtaaaaaaa
     9061 gaaagtaatt tataccaaat ggcatagagt gggccccagt cgcgattaag atgtcccggc
     9121 aaatccaagc caaaacgcaa gtaagctgct tcatcggcaa actggatatc catgtgattt
     9181 tctaaacctg cataaatgtg cagaccgcaa taaaccagca ccccaatacc tgcgataagt
     9241 tgccaaattg gagtagtctg cttcaacgga acatcttctg caaaaattga aaccaagaaa
     9301 ttttctagtc gttttagcat actcattgtt taatcagaaa ataactttca cattcattac
     9361 tatattccac tttattaaaa ccaaatggct ctggatttcg cataaattga ttccatgcag
     9421 tatcttttaa cagaatcgga ttctttataa tattaacact gactaagata atatcaattt
     9481 ttctttcgtc taaaatttgt ctaaaactca ttccctcttt tagttcaaac tctgtactga
     9541 attccgaata gtttttaggc aatatatagg ttacatttaa gtagttggtc attaccacat
     9601 gcgggtgtgt tttatccttt tcaagaaaac gaatcaattt ttgttcgcac aaatttttca
     9661 tatcgcttcc aaattgcata taggcataac tcgaactctt cggagaaata actataaaaa
     9721 gcaagaagaa tagccaatac gaccattgca tatttatttc caatgtaatt gaatccaaaa
     9781 gcaaggcaat aataaaggct aataagataa tgtgcaataa gacatagtgc attcgtggaa
     9841 aaatcactat agacataccc aaactgggaa cgccaaatat aaacaagaac aacaaaagct
     9901 gtttattatc ctggagttta acaaaaaact ggtctctgta ttttttgttg gcgaacacta
     9961 caacaacaag caacaacaag ataaaaactg ataatatgag tgcttttttg cttttaaaaa
    10021 tacctgttgg aaaaacaaaa gaagttaccg taagcaacag tatcatgaga tagtttttac
    10081 aattaagcag tagattttgt aaaaacaaac ccggctgatt cagtatcacc tcttttaaag
    10141 tatggctgtt tgggaattgc tgtttgctaa agttaatcca ttcggtaatg gcatcaaatc
    10201 cgtctttatg catcatctta taattcatgg catagtgctg acaaaacgca ctatacaatc
    10261 tgtccagccc tcctttgtaa gtggcagatg gctgcccaaa aacaaactga aagaacaaaa
    10321 cgacaataag caatataaat aaataaggca gaaatgattt aatcgcattt ttttctttcc
    10381 aaatgaaata aaatgtgaca ggtacacaac aaaaaaacga aagcatcagg tcaggacggg
    10441 catatgcaca gataaaacat gctatcgagt atatcaacca tttttttgcg ttggaggatg
    10501 taagcgaagc caatatcaaa gacgttagta aaatgataat tacaaaatgt gaaactcgtg
    10561 gccatgtgtt aatatttatt gaagaaacta agaaacaaaa acctacgtat agtgctactt
    10621 gtttttgttt tatgtgttga tataaaaaga caaaaaacaa aagaccaatc aagacagaac
    10681 ctaaggcata attcagataa tagagttgta ttgtatcact cttaaaaaat gacaaaaatt
    10741 tataccagat attataagcc ggaccccaat catttctgat aattttgaat aaatccaaac
    10801 cattgcgcat atactctgcc tcatcgccaa acagaatatc catattgcta ctcaaatgca
    10861 gactccattt tattccgcag aaaagcataa atgccatgcc tgaaataaag tagatctgtt
    10921 tttttagcaa ctgcatgata ttgcaaaatt aaggtttcac caacaaatag ctttcacaac
    10981 tatcacaata tatttgtttt tgaaacccgt agtcttcagg atgagcaaca aaagaagtcc
    11041 agctggtatc ttgttgcagc agcttatctt gtagaagaat atcgcgcatc aacacgtggt
    11101 caatcttctt gtctttaaga atagctgaaa aatgcatacc cggcttaaac tcatactctg
    11161 tattaaaatc agcataattc tcaggcagca tcatactcaa acttaaatgg ttggaaaaaa
    11221 taacatgctg atgatgattg tacttttctg tcagatagtt aactaattcc ctgttgcatt
    11281 tctgtttatt atctctatcc atttggtaat aggtatattt attcgctttc ggcgatttat
    11341 agataaacgg gatgaagaac aaaagtagaa ggtaggattt gctttttgta aaaaccggga
    11401 gtgcgttgat taacaaagca accaggaata taagtaacgg cgcatgcatc agtaaataat
    11461 gcgcacgtgg aaagataagt aatgaagtag ccatactagg caaaccgaag atgagtaaca
    11521 cgagaagtac cagtttgttg tctttcaggg attgaatgaa tttgtttctc gtttctttgt
    11581 taagaaaaac gtaaacgaga attccaatca tgaaaaacca gatgagcatc atcaaccatt
    11641 ttttagccgt aatttttaca gggaatagta tggaagtaaa aaactccaat agcaccaggc
    11701 aatacttttg gatattcaaa atagtattgt gtaatactaa cccgggatgt gattttaaga
    11761 tacaactgaa atttttacag tctccaaaag tacgatgtgc aaaatctatc cattctgaaa
    11821 tgggattaaa atttgctttt gtttccaaca catagttaat ggcataatgc tgacaaaatg
    11881 caatataggt gcggtttata cccttgtaac tgtcggcggg taattgataa ataccgaaaa
    11941 aaacaataac aatgactatc aaaaaaacaa atgccggcaa agctgatctt agtgattccc
    12001 gaaatttaaa atagatgaaa atggtgagta aacacatcaa taaaaaagaa ggaaataatt
    12061 cgggacgtgc ataggcagta ataaaaaaaa cagccgttag ccatacagac tttgagagat
    12121 atgttttaca attccgaatg gcgataaaac ctaagacaag caatatgaat gcaaaatgag
    12181 aaacccgagg ccaagtatcc atgatggtag tggaaaacat aaaacagaat gacagccaaa
    12241 aggctatgat gacatgaatg ctataattcc gcaagaagat aaataaaagc aacgcacaaa
    12301 gtacagccgt tacctgataa ttgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnncgcaag aagataaata aaagcaacgc acaaagtaca gccgttacct gataattgaa
    12481 ataaaaaagc cggatattat tgtgttgaat taaggacaac agtttatacc agatattata
    12541 gctgggaccc cagtctttat gtatggtatg aaataaatcg gtgccatatc gcagatactg
    12601 agcttcatcg ccgaaggtaa tatccattac agccggaagc cccagagccc atttgatacc
    12661 acaaaacagc aacagcagta acccgacaat aaaaagggca ttatttttca tagaaaatta
    12721 gctatcaagt taagcaaaag atgcatttat gccagttttt cagatttctt aataattctg
    12781 tctttcagct taaacatggg cacttccaca tacttaaaga gtaaggtacc catccaaacc
    12841 gttgaaccga tataaaatac acacccaatt atctgagcaa ttggcattgg tatatattta
    12901 gatgcaagtc cagttaaaat cagaaagatg attagaaaga cgaactgaaa gatgtatatg
    12961 ctgtaagatg gcaagcccag gactaaccag aactttttag tcagaaattt cagtttatgt
    13021 ggaaaataga caattgtcat gattaagctc aacacacaaa gattagtcag aatatctaca
    13081 aaatagatac caccattact gtatatatac ttatcttctc ttaaggcaaa cataaaagaa
    13141 accccatcat ggcgtgaagc aataaaacta cttaagtaaa tccaacatat tagtacagtt
    13201 agactaatgg ccagactcct aaatggattt ttccatttac ttaaatcaaa caagtttggt
    13261 atcagcccat aaaagaaaac atcaaattgg aatatagagg catgtacaac tgctaatgta
    13321 ctcaaggtag catctttact gtgtgctaat agccaattat atccgattac tcttaaaata
    13381 ggaaatatga atattgcaac aattgtcata attctcaata ttttcttatt aagaaagaaa
    13441 atagcaaaaa caataaaaat ataaaattgt tcttcgatag agacagacca caagtgaacc
    13501 attaccggag cagtttttat ataatcatca taccccataa aataggcata aatatctctg
    13561 taattagcta caaaagcaaa atgcatccat ccatagtgtt tccattcatt agccaggttt
    13621 tgaggaactg taccgaacca ccagaacaag aatgtaacaa atgcaatgaa gaatatgtat
    13681 aggaaataaa ttgggaagat gcgaagtgta cgtttgatat agaagagttt gaaatattta
    13741 ccaaaatttt ttagtttttc tttatcaacc attaaatttc gggatattaa aaatgctgaa
    13801 aagaggaaaa aagcatgcaa tgccacataa aacaatacgg agggaaagtt aaattttgag
    13861 aatctataat gcgctaacat aacgagcaag caacagaatc cttttacgcc atctactact
    13921 accaaatgtt ctttcttgtt tatcaattta tttgattttt agcttttgta agataaggtc
    13981 ttttttgtta atacaatata gctcatattt agtgtaaatg aagtttgaga aacgaagcaa
    14041 taaaaaaatg attaaacaag tcgccaagat ttctacaatt aaatcataac caagtagaga
    14101 ttttaaataa ggaactagat aaaatgcgat tggaaatata aataagtact ggtatacata
    14161 aactccatat gtatattttc caacagaaat gaagaaagga atagtaaata acttgaagta
    14221 tgattgcttc tttactgcaa taaataccag gtagaagcaa atcatattta ttaatacatg
    14281 atagtaatat cgatggaaac cttcaaccgg aaagatgtaa gaatatagat tatctactat
    14341 tgatttctta ttgtacaagg ttagataata tgcattgccc aagcacgaaa aaacgaatac
    14401 aataagcaat acataaaaaa ttcgaatatc acgtttcttc aagtccgggt taagtacagc
    14461 caataataca ccataaaaga atgtatcaaa ttggaaaaat gttgatttta taaaaataaa
    14521 agatggcaaa taagggtcat ttattatgga taacatataa tctactccaa taatcctgat
    14581 aatagcaaat gcaatcatca tgaatatgga aatccggatt agccattttt tagaaaacat
    14641 gaaaattaaa aacgggataa tcaaatagaa ttgctcttca aatgagatag accatagatg
    14701 tggaaatgca taacttgctt tgtagtttaa cccttgaaac cacgcccaaa aatctttaaa
    14761 gttatacgta aaactaacaa ccatccaacc aaaatatttt atttcatacg tgattcccaa
    14821 tttttgtttt aagggagaat gggcagtaat aattccaata aatagtatta taaaaaaata
    14881 tagaaaatag gtagggaaaa tacgaagcac tctctttgta taaaattcaa taaagaactt
    14941 tttaaaacta tcgtgtttat ttttagcgta taacagatta actgtaatta aataagatga
    15001 catgacaaag aaactatgta acaatagata acctacaata tagggcagtt ttaagtatgt
    15061 gaaatgaaaa tggcaaaata agatacacaa acaaaaaaag cctctcatgc catcgagact
    15121 acttaggtaa ttcttatttt caatcttatt cataaaaaag ggtgctgaaa tttcagcacc
    15181 caaaaatact ttattccttt ttatctttaa tgagaaatat tgatggtctt tacaattgca
    15241 tttccaccaa attgtgatct cacaaaatac attccggcag gcaacccttt tgtatttagt
    15301 tctaacttat tagtacctaa ttgtaaggat ctttccatag tcatcacaat agcacccgtg
    15361 gagttgtata tttcgacctt aacatgtccc tccgtttctg aatatagttc aacatttaag
    15421 agattatctg atgggtttgg atacaaagag aatgacttat ccacttctga tttttgttct
    15481 tcaatgcctg ggcttgccat ccttgtatat gtatttcctg agttcataca tgatgtacta
    15541 ggtacaatac ttccatccat attggctgag cttactctat accagaagca aatctgattt
    15601 ggaggagttc caatatagaa agggttaact gaaaaatcgg tattagtctt tacaaaattt
    15661 ggagtgctat ttggtggtaa agttggtgta ttgtttgtat tggtacgaat atcctttaaa
    15721 aagtttgtta atattgtatt tgagtgaatt attcctgaaa atgcatacat catctgagtg
    15781 ccaaataagc gaaatgaagc accatctact gaataattat aatatttttg ttgattgtca
    15841 tactttgtat ttacagggtc aatacagttt agaaagattg tatttccagg gtagctaggg
    15901 tctgtagttt gaattgtatt tcctagagta agaatatcat cccttgaatt gcttgttgtt
    15961 aacattgaca atttatctgt taaagtattt gttaccagac aactaagggg agtaacattt
    16021 ccagtggcaa aatttttaaa aaaaagatga ccaccattac agtttacaaa caatctgctt
    16081 aaaataccca atgatttcat agtagttcta acactattaa atggcagtaa taaagcagaa
    16141 cttgctggat caggaggtaa aaagtaatta actggcaaag atgttggacc atttattcgt
    16201 acatcatcct cgtttcccca caaaaataag gttggaacac tctttggcgt tgtagtaaaa
    16261 taatcttgat ctgccggcac gtaagaattt cctaaaatta acgatttaat atttatacta
    16321 cttgtagggt tagcatttaa gcatagtttt gtatagttat tattagaaag ttgagtatta
    16381 ctgcatgtat gcgtagatgt tccagtataa aagagattat aattatgtgt cgtataatta
    16441 attccggctc ttgaataggc taacatgaaa gcagtagacg ctcccatact gataccaaat
    16501 gcacttatat catttgtatt ggcatttatt attctttgag ttgcgggtaa agaagcatct
    16561 gtcaaaatat agttataagc tgcaacagca tactgaaaat tggcataagc attctttctt
    16621 attacatcat caccctgtcc ataataatta cacggatatt gatttggcaa aatgtaagca
    16681 agtccagcta aatcattact taagctttca taatacacaa cacaatatcc ccttatagca
    16741 agatcagcta ttattgccca caccgcatag ccacctggtt tagcaaagtc aatcatccaa
    16801 ttagccccat ttgtaatcat aatgcaaggt cttactacat tatcatctgg tcttactacg
    16861 accattctta cgaaaggatc cggatttcct gttacgtgtt tctttgcata cattactttt
    16921 ctggcaatta tcccatttga ttttactata cggtcatatt tatatgctgg agtgttcaat
    16981 aaaacatttg tatctgttgt aggagtataa ttcctgctat ctccataaaa tgacatcaaa
    17041 ttgttttgaa tttttaaggt taggttggta agtgtggcca agtgtttttg tgtattattg
    17101 taccccgttg ttccataagg tgagaaattt gcactgttaa cagctacaaa attcgggtca
    17161 taagcggtca tgtaagttgg cgcatattgc gcatgtgtga ataaacagcc ggaaaataat
    17221 acccagcata gcataattgt tttcattgtt gttttcataa attaatattt aaattgataa
    17281 aaaagggtgt ctgtattgtt ttgtatattg cgtttggtta atttattatt attatagaaa
    17341 tatccatatt tgtaggagtt aacggtgtca ccattcagca gattaatttc ttttctatcc
    17401 agcaaattat tcatgtttgg aatacctata tttaaaccta aaagtggaat aatattaacg
    17461 cctccggtat aaataacttt aataccagat gaataaaatt ttaaatcaaa ttctgtgtca
    17521 agattttcag ttccatattc catgtatatt cttccaattg gaatgtgatt ggcttgtgct
    17581 atgtcatatt tgattaagtt attattaata tcatattgaa attggaagtt tacagcataa
    17641 ctagtatctg taataagagt aaatgaatca ataagatttc tataggaata ataacatctt
    17701 agcccaaaac cattagtatc caaaatagat acaacctgtt tgttcgcatt aaaattaaaa
    17761 tctactctga aatagcctcc ggtaatatga tatccatgta caattacttt agtcggggta
    17821 tagtcaaaaa atacatcttt tatcagtgtt gcatcgtaag tagtatcgtc ataaacggtt
    17881 agcctgttta atgttccatc attattataa tagaaatttg tagactctat gatgtttccg
    17941 ttgctgctta gtctcccgtt tacttcatct tctactaccc aattcgattg tcgtatttta
    18001 ttattttccg gtttagtgtt atctatccgg ctgcaactga ataacacaaa gcaacttgca
    18061 ataataaaac gactgatata ctgtctaagt attgacataa atgggcattt gattctcatg
    18121 ataaaattgt gcaatatttt taatatttat ttacacaaat attccaattt gtctcttaaa
    18181 tttgtatcta tattatctgt agtatgaatg aagctaaact atattataca ataaaatcat
    18241 tagaaactaa tgaaattagt cgtctaaaaa aatcactaaa tgacaaattt gtcgcaaata
    18301 attcagcatc cataaaacta attactgcca ttattttgga aaagcatcta actgcacaag
    18361 attggaacaa gcaatttata catacgttaa tttatccgaa tttaaaatat aacgatttaa
    18421 gaataagaca cttactatcc aatagttgta agattcttga gcattttatc attagtttaa
    18481 aaaccaatga aaataaaatt ttattaaatg aacaattagc agatttttac ttatcaactg
    18541 gcagtatcaa atatgcagaa cagtatatcc atgaaagtat attatctata atacataaac
    18601 cgaaaaattc gtctgcctat caaagattat ttgagctgga aagcaaactt tatgatatac
    18661 agttgaaaaa cagcagaaaa acagataatc atatattgaa catggcaaat aatctaagta
    18721 tttatctaat tattgaaacc ttaaagactg cttgttatat tgcatccaat aatgttataa
    18781 ttgacgagaa aactgacttt cctgtaataa aaccgatata ccaacttgcg caagaagaga
    18841 tctattatga cattccccaa attaagatat atgcaacatt atatcgtctt ttgttgaatt
    18901 ggacaccaaa agaattcaaa aaaatattac cctttattaa aaagaatgaa tttctattta
    18961 aaaacgatga actatataat atatataagc tattgttaaa tttttgcata aaagaagcga
    19021 atacaaatag tcatgagttt acaaaaatca catttgaatt atacctttat gcaattggta
    19081 aaaaatattt attgtcttat ggacaactaa acagatttac cttatctaat gttatcgcaa
    19141 ttggattaaa attaaaggac atcaaacggg cagaagattt tttaaaggca aattatcgtt
    19201 ttatagacaa aaactttaaa ctaatgacct ataattataa tatggctaaa atattatatg
    19261 caaaaaacga aaatcagaaa tctttaaaat tattgctctc tacagaattt aaggatgcat
    19321 tatggtatct gaatgcaaaa tttataacct taaaaatatt tctggatatg gatgatatgg
    19381 tcagattcaa tcagtatttg aaagcattta aaatgtatat cataagaaaa caacatatcg
    19441 gctatcataa acaatatttt tcaggagtag caaacgcatt taaaatttta ctgaaaatca
    19501 gaaaaaactc actaaaatat caatcctatt catttgattt agaaactcct gacagagttt
    19561 ggtttcaaaa caaagcaatg gaatatgtta atcaaataaa aactaaaatg caacacccct
    19621 aatcctctct cactatctta ttatagactc tttgtgatat accgatactg acctgatata
    19681 aaataaaaat tggcgtggaa attagtacca aactaaacat atcgccctgt ggtgtaataa
    19741 cggcaatagc acccaaaatt attatataag caaatttccg tgtgttgcgc ataaaagccg
    19801 gtgtgattag cccgatgcgg gtaagaaaat aaaccacgac aggcaactca aacataatac
    19861 ctgtaggcaa taccatagaa attaaattgc tgatataatt atctacggaa atcagattct
    19921 ctgcttgccc tacggcttca taattcaaaa agaaattaac ggaaaatggg gcaatgataa
    19981 aatacccaaa catacatccc aataaaaaca agatgatgac aaaaaacaaa acacctgtgg
    20041 ttttcttaat ttcctgctca tagagtgccg gctttataaa ccgccaaatt tcccaaatca
    20101 catacggtga agctataatt gcacctgaaa tggctgccat ttcaaaatgc agataaaact
    20161 gccccgccaa atcagtattg ataaacttat gtacttttcc ctgcatacat agtccgctaa
    20221 aaaaataggg cgactgctga gccagccagc agaaaaagcg atttgccgga aaatccggtc
    20281 gcgtgggacc aaacaacaga taatcaaaca agaatttttt attggaaaac atgaacagag
    20341 acgctgtagc tatggctatc aaagagcgta taatatgcca gcgcaattct tcgatatgat
    20401 cgaaaaagcc catttctgcc ttaacattgt tctttaccaa ttttcccatc ggactgcaaa
    20461 ggtaaatgtt tttttagata tgggtagtga gtagtgggta atgagatttg tagaatcaag
    20521 agccaagagt caagactata ttctttgtaa aatcaactgt ggactatggg cagtggactg
    20581 cggactttct ttgagaatca agagccaaga gtcaagaaaa tatacccaat caataatcac
    20641 taatctctaa tcaccaataa ctaatgacta atctccaatg accaacaacc aatccctatt
    20701 gcctaaaaaa tcacctcttc tttaagccca gattctctag tttctcttct aattgtttct
    20761 tggaaattcc gatatcgtca gccgttttgg aggtgttcca ctcattttta ttaagtttat
    20821 gttcaataaa actgcgttca gtataatctt gtatctgctg caaattggca aatttatcat
    20881 agtcaattcc cttgctttct gccttgccac tcggtccgga gtaatccaac acatcctgtt
    20941 tggtaattgt cttatcactc ataataatca atcgctccac tacattgcgc agctcccgta
    21001 tatttcctgt ccagttgcgc gcttgtagcg tttccagtgc gtcattattt atttttttca
    21061 ccggaatccc atattctccg caaatttctt gtaaaaaatg atcgactaaa accggaatat
    21121 catcacggcg ttcattcagc gagggcacat gtattaaaat tacactcaga cggtggtata
    21181 aatcaaggcg gaattttcct tcctccactt ctttaagcaa atctttattg gtagccgcca
    21241 ccacacgcac atctactttt tcctctttat cgccaccaac tttagtaatt ttattttctt
    21301 gcaaagcacg caatactttt gcctgcgccg acaagctcat atcgccaatt tcatccaaaa
    21361 acaaagtacc gccattagct tgttcaaact tgcccacccg ttgttttatg gcagatgtaa
    21421 aagccccttt ttcatgcccg aacaattcgc tttctattaa ctctgacgga atagcggcac
    21481 agtttacctc cactaacggc ccgccagaac gattgctttg ttcgtgtagc catcgagcga
    21541 ccagctcttt tccgcttcca ttatcgccgg ttatcaatac acgggcttct gttggagcaa
    21601 ctttagctat ggtgtctttt atttttgtaa tgggtgaaga aatgccgacc atatctttcg
    21661 actttgcaat tttctttttt aagatttttg tttcagacac taaagaaact ttatccatgg
    21721 cattgcgcag tgtaatcagc agcctgttca aatccggtgg tttgggaatg taatcaaacg
    21781 cacctttttt aactgcctct acggctgttt ccaaatttcc atgacctgac aatacaatta
    21841 caggagtgtc taagcccttt tgttgcattt cagtcaatac ttcaatgccg tctttttttg
    21901 gcattttaat atccaaaata atgacatcat aattgccttt ttctattttg gacaatccgg
    21961 cttcaccgtc ttcggcttca tcaatctcat acttttcata ttcgagaata tctttcaagg
    22021 tgcggcgaat ggcgcgttca tcatctataa ttaaaacttt aggcatatgg atataacatt
    22081 tttaaattca aaatctgttg ctgacaaagc taaggaaaaa ttatgagttc tttgaagtgg
    22141 gaaagtgcaa tcactatttc cgctcccagt agttcacctc atttatatca tgcaggatat
    22201 ttagataatt ttcatagcgg gttaatgcaa tcttgccgtc ttctaccgct ttgataacgg
    22261 cacaattagg ttcttgcaca tgcatgcaat tattatactg gcaagattcg gcatactttt
    22321 ttatctcgct aaaatagccg gatacttctt cgggctcaat atgtaatgtg gcaaattctt
    22381 ttatgcccgg cgtatctatt acatagctat tgtcaaatac ttcaaacatg gtggcaaagg
    22441 tggtggtatg catcccttta ttccattttt ccgagatttt tccggtcttc aaattcaaat
    22501 ccggattcaa tgcattgagc aaggtacttt tacctacacc ggaatgtccg gcaattaagc
    22561 tggttttatc ttgcactaaa tcatgaaatt gcaagatatc attcggattt tgtgcactca
    22621 aagctaatac tggaatgccg atttccggat aaatctgttg cataatttcc agttgcttgt
    22681 tgtctttttc agtgtgtaaa tccactttat tgacaataat aactaccgga atatgaaagg
    22741 aagctgcaga acaaataaag cgatcgatga agcccaaaga cgttcggggc ttagaaacag
    22801 tagccactaa tatagcctga ttaatattgg atgccagaat atgacgctgg tgcttttgac
    22861 ggggcgactg gcggatgata taattttctc tcggcaatat ttctgcaata acaaattcgc
    22921 cgttttcagt tttttccaat accacaaaat cgccaacggc tatcgggttc gtggtatcga
    22981 tatcactcag tttaaacttg cctttcagcc tgcaagtaat caccttaccc tctgcaagta
    23041 cctgatacca tgatcctgtt gtttgtataa ctctgcctgt cattgcaata tcgtgtgatt
    23101 aatcattttt tgcctgatcg gcaatttctc tttcaatatg ttttaattct actttcacct
    23161 tatacttatc tcggatatgc ttagctgttt gttcggcaca atatacactt ctgtgttgtc
    23221 cgcctgtgca accgaaatta atttgcagat gatcaaaatc gcgctctaaa taattttgta
    23281 tggtcaaatc tatagtctgc caaatggtat ctaaaaatga ttttacattc gattgtgttt
    23341 ctaaaaattc tatcaccggc tggtctttac ctgttagttg tttataaggc tcatatctac
    23401 ccggattgtg caagcctctg caatcaaaaa caaatcctcc gccattacca tgctcttctt
    23461 tcggtattcc tcgcttaaat gaaaaactat tgacttcaac aatcaatctt ttatgactgc
    23521 catcatactg aatatattta aaaatatccg actcggtcaa ttgctgcaat acgcttttta
    23581 attcaggcaa atcttttatg tgcggaataa aaatcttcag attttctaat gccggaatga
    23641 tactttgaat aaagtgagga cgacgttcaa ttaaacctcg gaaaccataa gctcctaata
    23701 cctgcaagag tctgattaaa acaaaaccct gatatcgctc tctaaaatca gcttcatcta
    23761 tggcaacgtg tttttttact tcgttcacat aaaagtcgaa gagttcttgc ctcaaatcca
    23821 taggtatttt tgctccggct tgccataata aactggcgca atcatattgc aaggctcctc
    23881 ttctaccgcc ttgaaaatct ataaaataag gttcttcatt ttttatcatg atgtttcggc
    23941 tttgacagtc gcgaaacata aaatagagat gtctttcgtt gagcaggtaa tccgtgagtt
    24001 tgtgaaaatc tttattgagt tctgtttcat taaaatgcac tctggctacc cttgcaaaat
    24061 aatatttaaa cgagtttaaa tcccaaaaca tactggattt atcaaatgca tttatggggt
    24121 aacatttatt aaaatcaaaa tcttctgctc ctattatttg caaataagcc agtcgttcta
    24181 aagatttttt gaacaagctg atgacacgtt cgctcattcc ttcttgctga attactgaat
    24241 acaaagacac atcccctaaa tcttcttgca aataataatg gtggtcttga gaggctgcaa
    24301 aaacttgagg tactgctaca tctttactat gaaagtgcac cgccatgtaa ataaatgttt
    24361 cattctcttt tatatccgca ctgtaagttc cgactacacg gtgttcttta ttactcagac
    24421 gataatatat cctgtctgac cctgactgtg gcagtttatt aattacatca gcttcgtgat
    24481 taaagctaga ttgaaagagt gcttttaatg ctgtttcgtg catagctcaa acatacagaa
    24541 aatatgggaa aagtgaaagg caagatgaga acgaaggtaa attcaaccag taactgcttg
    24601 tcaagaaata attataaaat aaactgatac cgcattgaac cctaaaatat tagttacttt
    24661 gtctttataa aaaattgttt tttacagaaa ataaaatgac atcaagaaag ctcatattag
    24721 taggtgcagg tcccggcgat cctgaattaa tttctctaaa gggtattcgt gctttgcaac
    24781 aagctaacgt ggttatgtat gatgccttag taagtgaaca attgctggat tatgcaccaa
    24841 atgccataaa aatcttttgt ggcaaacgag ccggttcaca ctacatgaaa caagatgaaa
    24901 ttaaccaact gatagtagag caagctgaaa cacacggcac agtggttcgg ttaaaaggtg
    24961 gcgatccttt tgtttttgga cggggacatg aagaaatagc ttacgcagaa aaacacggaa
    25021 tttttacaga agtcattccc ggaatttcaa gtgccactgc actttcttca caacataaaa
    25081 ttccgcttac acatagaggc attaccgaaa gtgtatggat taccaccggg actacctctt
    25141 gtggcaaact ctctgatgat attgaactgg cggcacaatc caatgcaaca gtagtgatac
    25201 tgatgggcat cggaaaaata gatgaaatca taactatttt tacaaaatac aaaccgcaag
    25261 attttccaat cgccattatt caaaatggaa ctttagccac tgaaaaaata ataagcggac
    25321 aactcaatac gataagtgaa tgggtaaaac aagaacaaat aagtgcgcct gccgtaataa
    25381 ttatcggaaa agtaacggca ttaaataatt tatcatttcc acaaacagaa aacgaataaa
    25441 ttgaacacgc tttatcccat atttttaaac acagcaaaca tcaggatatt gcttgtcggt
    25501 ggcggacata cggcaaaaga gaaattacat tttttgctaa aaaactctcc ggatgcccaa
    25561 attacattag tagcaaaagc agtcatagca gatattttgc cggatgaaaa tcttgcaaaa
    25621 aatttcgtta cgattctcca acgagaattt gaacccgctg acctgcatgg aatacatatc
    25681 gtcatagcgg cgacagataa tcctgtttta caccaggaaa tttatcaact ttgcaaaact
    25741 aaaaacatcc tgatcaatgt ggccgacacg cctgctttat gcgattttta tctaggcagc
    25801 atcgttacca aaggggatat aaagattgct atttctacaa atgggaaatc gcccacttta
    25861 gcgaaacgtc tgcgcgagta ttttgaagac atattacctg atgaaatagc aacattagcc
    25921 gataatctga acagctaccg gaatgaaata aaaggcgatt tgcaagataa aatacgcaag
    25981 ctgaatgaat tgacacggtc tttcttaaaa ccgaaagaat aattatattt aaacatggaa
    26041 aaaatacaac tccctttact tagccgaacg atggatgctt atttttatgc ggcttcagga
    26101 gaagaaaaac gccccggcat cttatttctt cccgacctga ccggcataca gaaaaccaca
    26161 cataagtctg caaagatttt aagtgaagaa ggcaatttca atgtaatgat tcctgacttg
    26221 tacagtggag gtgtcaatat gtcgaaatac tgtatgcaat tttttttcaa cggtttggtt
    26281 cgcaataacg agcctacagg caacgccccg ttaattgaat tttttgagat tctagaccaa
    26341 ttcaaatcat tccaggaagt agatgcaaac gcgattggcg ttgtaggcca atgcctcacc
    26401 gggggttttg ttcttcatgc agctattcgc cctgaagtga aagcaccggt tgtttttcat
    26461 cattcatttg gtcaaaaagg cagcggtatt cccaaaagct gttcggctat ggtgcaaaat
    26521 aaagtacagg gtcattttgt ttatgttgac ggcttttgcc cggtgtggcg tgtacgaaaa
    26581 ttagaaaatg aactcggcga aaatctggaa acgcattggt ataatttgcc gcacggtatt
    26641 ccgcatttgt tttttaataa tgaacaaggc aaaaaggcgt ttgaacgcat gtgtactttt
    26701 tttgaagacc aattaaattc gtgacagtga aaaatgcatt taaaaacata ttgatttttc
    26761 cttttatctt attgattaaa gtctatcaga tcggcctatc gccgatgctg gggcccaaca
    26821 aatgccgtta tcagcctacc tgttcaaatt atgcgcttga agctttaaaa aaacatggtc
    26881 ttttcaaggg cggctatctg gcaactaagc gaatattatc atgccatccc tgggggggaa
    26941 gtggttatga cccggtgcct taaaaaatat taatactatt tgatttgtac aaaattatcc
    27001 gtgagtctgc gtaattctat ttctgctaat tttgctgtat aacgtgcaag aatcacattg
    27061 aacaatgcca tttcataact gctttgggca gttttcactt ccaacgttgt tgtttggttc
    27121 aggcgaaaac gctgcatgga taattgtaca ttttcatcgg ctgtttttac ggcatcttca
    27181 ttcaatttca aaacagtttt tgcatactcg tagtttttat aagcattata cactttttgc
    27241 tgtaagtcgt tttttagttg gctaactacc agttctttaa cggcaatatt aacggcactt
    27301 gcttccaatt gtttttttat aataccacca ttataaatgg gaataacagc atttatcccc
    27361 actgttggac cgtatgatcg attgaccaat tgcaaacctg ccttgctgtt attctgattg
    27421 aaattatatc cggtactaaa atttataatt ggcagttttt gtgctgtaat ttctttgtgc
    27481 tgtaatttgg caatatctat atttttttgt gcagataaaa tgctgaaatt attcaaagtc
    27541 gtatctttca gttgttgtaa gtcggcttga taattcatag caaacgtatc aatcacatca
    27601 aaacctgttt ctgcatctct tgatagtaaa ttatttaaaa cggccttact ttgttgtaaa
    27661 acaaaatcct gttttacgat agctgctttt tgtgttatta aatcaacctg agcttgtaac
    27721 aaaggtgttt tgtcactata acccacgtct agtttttttt gtgataagtt cacgcgttct
    27781 tcatacaatt tcacccattc atacatggct ttgagctgtt gtttttgtct tacaatttca
    27841 aaataagccg ctaaagtttg ggcaattgta ttttgtattt gctctttcaa ttgcagttcg
    27901 cctaagactt gcaaggcttg taatctgtct ttcgttgcaa acattttcaa tccatcaaaa
    27961 gcagtccagt tcagattcag tccggcatta aaactattta caggcaccca gtttttattg
    28021 gttaccgttc cggtacttaa ttcttgatgg atattactca gattaaatac atcgcccgtt
    28081 gttacattta ttttgggcag catacctgca gctcctttgt tgttgttgat gcgcaaaata
    28141 tcagcttcat tttttgccaa aataatatcg taattatttt ttacggcata agcaactgca
    28201 tcttccggcg tgagtaaatc ttgtgctgat gctgaaatta tcaggctgag caagtataat
    28261 aatgctgtta tctttttaaa tctggtcata tttagatttc gctcattaat aaaaattatt
    28321 ctatcgtatt cgcttgcaag gcttcttctt tttgttccga cttagtacgg gagaaaaaca
    28381 catacaaagc cggcacgaca aacagtgtca ggattaatga aaacatgata ccgccaataa
    28441 tgacaatccc cagagaagtc ctactggtag agcctgctcc aagagccatt gcaataggta
    28501 atgcccctaa tgccattgcc aggcttgtca tcagtatagg acgaaggcgc atagatgcgg
    28561 catagatggc agcatctatt tttgacattc ccagtctgcg attgtgattg gcaaattcta
    28621 caattaaaat cccgttttta gtcaccaaac ctatcagcat aatcattccg atttgactga
    28681 agatattcag tgtttttccg aatatccaga gggataaaag tgcgcctgct aacgccagcg
    28741 gcacagtaat cattatgata aacggatcga caaaactttc aaattgtgca gctaaaacca
    28801 aataaattaa aaccaatgcc agaattaatg caaacatcgt attggaggaa ctttcagcaa
    28861 aatcgcgcga tgctcctgtg agtgaagtgg taaatgaggc atccagcact tttttggcta
    28921 tcttttccat ttcctgtata ccgtcgccaa cagtctttcc ggacgccaat ccgcttgata
    28981 tcgtggcaga tttgtatctg ttgtaatgat agagttgtgg cggtttggag taagttgaaa
    29041 ttgaaattac attgtccagc gaaattaatt ttccggcatt attgcgtaca aagattgatt
    29101 ttaaatcatc cggcttattg cgttccgcat acaaagattg tcctaaaatc tgatattgtt
    29161 ttccatttcg caaaaaatag ccgaatcttt tcccgcttag tgcaaactgt aaagtttggg
    29221 cgatatcttg cgtagtcaaa cccaagctca ttgctttttc tttatctatg gtaatatcca
    29281 cttcgggttt attaaacttc agatttacat ccgccccgac aaaaacagga ctatttgaaa
    29341 cctcttccat aaatctggga actgcctgct gtattttatc aaaatcaaaa ttttgcaaaa
    29401 caaattgcac cggcatgccc atacgagctg aacctaccga gatggtttgc tcctcaatga
    29461 caaatgcttt tccaaaattg aacttcgcaa aatttctgtt taaagattgt gctatatcac
    29521 tttgcgaccg tgtgcgttct attttgtctt tcaggcgtat gcgcataaaa gcagagttcg
    29581 ctgctcctga cccggcgaaa cctggagatg ttacactcag aacaaccttt tcttcaggta
    29641 ctgagtccgt tacaaactgt gataaattat ctacataatt ttgcatagcc ggataagctg
    29701 taccttcggg tgccgttacc tgtacccgca aagagcctct gtcttcaagt ggggcaagct
    29761 ctgtttgcag atttttcccg ataaaataaa tcaacccaaa tgagattaag acaataacaa
    29821 gggcaattcc tcttaccttc ataaaagaag tcagccattc tttatagcca tcatccagtc
    29881 ctttaaacag aggttctgtt ttgtcgtaaa accaggaatg ggtatgtacc tttttgccta
    29941 agtacacatt gagtaccgga gtaagtgtaa gggatacaaa agcagaaatc aatacggcac
    30001 ctgatacaac aataccaaac tctttaaata atttccctac aaagccttgc atgaagataa
    30061 ccggcagaaa gacgatggct aaggtgatgg acgtagaaac gactgcaaaa aagatttctt
    30121 ccgatccttc tcgggcggcc tgcatgcgag gcatgccttt ttctatcttt ttaaaaatat
    30181 tttcggtgac aacaatacca tcatccacta ctaggccggt tgctaatact atcgctaaca
    30241 gtgttaatac attgatggtg aatccgaaaa tatacataat aaaaaacgca cctattaatg
    30301 aaaccggaat gtccagtaat ggccgaagtg cgattatcca atcgcggaaa aagaggtaaa
    30361 taatcagcac tactaaagca atagaaatca gcaaggtttc ttttacttct gaaatggagt
    30421 ttttgacaaa tactgtatta tccagagcga tattcagttg aatatctgcc ggcacttctt
    30481 tttttatttt gtctaacctg cggtaaaatt catctgcaat ctctacatag tttgcccccg
    30541 gttgcggcac taatgccaaa gcaaccatac cgactccctt ttcacgcaag ctggtttcat
    30601 agttatcggt acccaattgt gcatagccga catcttttag ccgaactgtt tgtccaccta
    30661 catcttttaa tatcagatta ttatattcca attctgtatt aagtctgccg agtgcattta
    30721 cattgagttc ggtatttttt cctgaaattt tacctgaagg taattccaca ttcgatttat
    30781 ttaccgcatt ttgtacatct ataggggtaa gctggtaagc tgataatttc tgaggatcga
    30841 accataaacg catggcgtaa tttttttgtc cccaaatctg aatcgtactg acaccgggta
    30901 tcgtttgcaa tcgttgttgc aatacattta cagcataatc attcagttcc aatatattcc
    30961 ttgtatcact ttgtaccgtc atggaaacga tagcgtccga attggcatcg gcttttgtaa
    31021 ccacgggagg ggcatctata tcttgcggga gctgccgcat ggcttgagat actttatcgc
    31081 ggacatcatt ggcggcagtt tccaaatcag cagacaaatc aaactcaacc gtaatattgg
    31141 aagttccctg attgctggca gatgaaatag ttcggattcc ttgcacacca ttaatggcat
    31201 tttccaatgg ttcggtaatt tgcgattcta taatatcggc attggctccg gtatagctgg
    31261 tgcgtactgt aatcacaggc ggatcgattg acggatattc gcgaacgcct aaaaaggtat
    31321 acccaatcac accaaataaa ataatcatga tattcagcac gatggcaaaa accggacgac
    31381 gtaaactgac tgaaggtaat cccatatact gattttattg tatggtagat ttttgagtag
    31441 ttttagtaat ctttacaggc atgccgggtt tcacttgcaa caatccggta gtcagcagcg
    31501 tatcacctgg ttttaaacca cttaaaattt gcacttcgct ggctgttctc aagcccgcct
    31561 ccacatttac aaacttagct ttactgctat cggctacaac aacctttgtt gccctatcat
    31621 ctggaataat acaattactg ggtatcataa tggcttgtgc tatatctttc aattttagca
    31681 tcactttcac atatgtgccc ggtaataatt gtgcatcagg gtttaaaatt gcagctctca
    31741 ctttgaggtt gcgggtagct tcatcaattt gtggctcaaa ggcatatact ttcgctagaa
    31801 atggttttag tatacctgcc acattacatt ccatcacatc tccctgatga atactcattg
    31861 cgtattgttc gggcattgta atatccatct tgagctgttg taaattttgc agcgtagcaa
    31921 ctaccgtctg cggtgaaaca taggctccca agctgatatt gcgcaagcct aattttccgg
    31981 aaaaaggagc tctgagttca gtttttgcaa tttgtacttt taagatttcg atatcggcat
    32041 tgatgttgtt gaccaaatta tctgccacat catactctgc ttgcgaaacg ccgtttattt
    32101 tcagtaattc tccaagcctt ttcaagtttg tttcagcaat tttcagttgc gcttttgatt
    32161 tctgaacttg tgctttcaag tcagcatcat taattttcat cagcaaagta cctttaccga
    32221 caaatgcccc ttctttgata ttgagataaa caaccctgcc tgaaacctcc ggttgtaagc
    32281 ttacttcttc agcggcgagc accgttccat tggcactgag ttcattggtt agttttgcat
    32341 actcaactac aaatccggat gcgaatactg ttttgctgcc acccttatct ttgcctcctt
    32401 tgggcatttc tttggcagcg gcctctttat tctttgtatg ttttacatac gccaagatgg
    32461 caattattgc caaaatacag ctgaaaaata tgattttacc tttagtcatg gttgtttact
    32521 tatcttgatg gaatgtataa atcgttttaa cgcattttta aaagtatctt cggcatgttc
    32581 ttttccggat cttatttttc ctaccatttg taaggaaata aatccgtgaa taatggcaat
    32641 aatattatca caaaattcaa tcattagttt ttcagatttt tttgcatctc gatttaattc
    32701 tgaaaaagct gatttataaa aatcaataac ggtattatat gtaggtttat cagttcctat
    32761 atcaatgcaa agtgcacctt gcaagttaaa cattacctga tacaactccg gattggtttg
    32821 tgcaaaatgc caccatgcca atccatactt taacaatcgt ttgtccggct tttcgtattt
    32881 tttatctatc tcttcaaata tttcatacat gctggtcatt gcctctatac gaatagcaca
    32941 taaaatcatc tctttgtttt cgaaatgctg atagattacc ggagcggtat aatgtatctt
    33001 atcacagatt tttctgatgg tcacattttg ccaaccttca ctggctgcta tttctttggc
    33061 ctccctaaga atcattcttt tcagagaatt ccttttatct tcttgtcttc gttttgcttg
    33121 catacttaac ttagttagct tagttaacaa agttataaaa tattctttaa atattatgca
    33181 tcactgatat tatcttaatc tgttgtacag tttgatttat agtttttgcc atatagaagt
    33241 atggatttat tttagaatta aaaaggttta ctttacaaaa attgagaaaa gaatagaaaa
    33301 atacagggat taccaaagtc gataatctat ttttgcatga cgatttatac gaatttcact
    33361 aaacagcgat ttatgattaa aaatgctatg tttctagatg gtttatactg cacaacgagt
    33421 tatcttactt acctaagtat ttagtatatc aaaattattc tatagttatc cacattgata
    33481 tacgtcaatt ttattaagat aaattcatct atttaaaaat aattttttat ttttaatcat
    33541 tgtttataca aattttacta tctttgtaaa gcaaacgcta aactaaacta caaaaggagg
    33601 ggttctgaaa agaacccttt tgttttaaaa atcacttgaa ttgggtagct tttatcattt
    33661 tctgttgctt aatttcatcc catgcaagta tctatttttg tatggatttg gatagcaatc
    33721 gctgtactga tttttcccat acagcttttt attaccgcgc cttatggtcg gcataccaaa
    33781 aaatcatggg gccctatgat ttctaacaaa gtcggatggt ttatgatgga agcatgggca
    33841 cctgtttggt ttgttattgt ttatttatta catccttctt atcagtttta caatttatta
    33901 tttgccagtc tttatctgct tcattatatc aaccggggat ttatttatcc atttcgtaca
    33961 aatacaaagg gcaagcaaat gccactggtt attgccatgt ctgccatgct tttcaattct
    34021 gtaaatgccg gcacaattgc atattattta tcgcgtgtaa gtgtttatcc ggataattat
    34081 tggcaatcat ggcattttat tgccggcgtt ttcttgttta tacttggatt tgtaatcaac
    34141 ctaaaagctg atgacatgct gattcatctg cgcaagcccg atgaaacggg atacaaaatt
    34201 ccgcgtggtt ttttgtttga gtatatctcc tgtcccaatc attttggtga aataatagag
    34261 tggctgggtt attttttaat gagttggaat ttggcttcat tatccttttt tatctggaca
    34321 ttttccaatc tttttccccg agcactcaag catcaccagt ggtataaagc ccactttgag
    34381 gactatccga gtgagagaaa agccgtattg ccaggagtgg tttaaagaat tataaattgg
    34441 tcggaaaata aatatggttg gatgtgctgg ataatatccg cccatcagac aaatgaagtt
    34501 taaataaaaa ccgaagcggt ttatattcga tattggtgta atcagtaaat agccgactcg
    34561 ggaagaagga tatattgctg atataatcct cattaatgga tattggataa gaaagttgga
    34621 tatatgaaga caagtctgtt tcggttgtaa ttgttgttga tatatcctga ctcggataga
    34681 tttcaatttg ccttatttta tcaacagagt aattacttgg aggactacag gcaaatgcag
    34741 ttgaaatcaa tccctgataa ggattagatt tatttgatac ataataataa tgaactggat
    34801 taattttaaa gtacatggca tttaatatag tagccgtatc taattttgta gtgtcaaaag
    34861 acaagtttgt tgttgcataa gatgtaactc caaattctgt aaccttaaat gtttttacat
    34921 caaaaggacc acattttata catgcagtaa actgactagc tattattaaa accactacac
    34981 tcgatgcaat gtatattagt ttcctcataa gatataaatt tattgatgct aatttaaata
    35041 atatttttca aaacctcatc taccctctct tctacttctg catttttctc taatactggg
    35101 gcatgatgcg gtttaatacg ggcatcaaaa ataagggcat cgcagctcca gtgtttgttt
    35161 tcaaaccgct cgttaatgcc atgtatatca tgcgatgggt tgcaacgtgt aaatgtaacc
    35221 catagaaaat tattgaaatc tgctgccagg aaatcggcat catcgcatac cactataagt
    35281 ggcagtccac tgtcctctgt ccactgtcct ctgttttgat tgataatagt accaactgtc
    35341 gactgtcgac tgtcgactgt caactcaact gctaaaattc ctttcgaaac cagcttaggg
    35401 tttttaaata aattcggcaa taaaaaatcg ttaggtaatt catccaataa atctcttttc
    35461 ttttctccac aagcagcaat tatcactttg cttcccgtat tcaagccatc gccactataa
    35521 tcgagtgtat ctatcgttgt tttggtctga aaatgtatat ctctcgataa atctatccgt
    35581 tctaaaacat gttgaaagaa ctttttttca tcgtgcgtgg tcaatgtttc atcatcactt
    35641 ttatccgcaa taaataaaaa tttggctaac gacaattgcc cggttcctaa aatatgattg
    35701 gctatcgtca ggatttcctg cggtcgtttt tcggtataat aaggcgtata gcgttcgcta
    35761 ccaattgcca gcaacaaggg gtgtactcct gcggcatcaa cggcatgtac ttcatgcaca
    35821 cccggaattt cttttgctat ggcatcacct gtcatttcgt gtatcatgtt gccaaaggct
    35881 gtatcttctt gcggtggtct gcctacaacg gtaaaactcc aaacagcatt ttttttggca
    35941 tatacatttt tgacacgcat caccggaaaa tcatgcttaa gtgaataata tcccagatga
    36001 tcgccaaatg gaccttcggg tttattgtca tttggatata cttctcccgt aatcacaaaa
    36061 tcggcatccg tacttaaaca aaatccttct tcatcataca tatacctgaa ccgtcgtccg
    36121 cctaatatgc cggcaaatgt caattctgag atgccttccg gcaaaggcat caccgctgcg
    36181 aatgtatgcg atggcggacc acccacaaac acagacacct tcatcggttt gcccaattta
    36241 ttccatttgg tttgatgtac gcctatacct ctatgtaatt gataatgtaa accaacctcc
    36301 ttatcttgta catactcatt tcccgaaagt tgtatgcgat acatgcctaa atttgactgc
    36361 atcacacccg ggctatctgc atcttctgaa tatactatgg gcaaagtaac aaaagccccg
    36421 ccatcatttt cccaacattg aatttggggt aaatcggaaa ttttaatttt ctcaaaagga
    36481 tgagatttta cctttttagg taaggcatta atggctttaa atgctaacct caatgcttta
    36541 agaggttttt ttagtgcatt aagcgggttg gtttttaatt caattaattc ctgtacgtct
    36601 tgtaaggtgt ctctaaaaat aaactgactg cgggctgtgg taccaaataa attggatacc
    36661 gctttgtatt tgcagccttt gatattttcg tacaaaatgg cttttccttt ggcttcatga
    36721 atacgcaaat gaatggctgc catttctaaa taagggtcaa cttcttcttt tatgcggata
    36781 agctgtccgc ttttttctaa atcgtctatg cattgctgta aagatgagta tgccattata
    36841 aaaattaaac agataaaatt acagatttta agcctatttg tgatgaaaaa cttaaaagcc
    36901 ataacgtaca atgttaaata aatcatattt ataatggatt taacaaatga atattataat
    36961 actttgtaaa tttgaaaatt atgaagtggg catttttctc atggctaatt ttatcaaaca
    37021 taatttctga aagtacaagc atcttaaata ctgaagttta tgtgccaatg aattcttctg
    37081 acgccggaat tatctatgct caggattact acacttatta ttttgcatat aaaaatattg
    37141 gcaacgagcc attgattatc tgtcatgtta gtggatcaag cggatgtgta tgtcctactt
    37201 attcaaaaga acccataatg cccggaaatt gggatactct acgtgtaaca tattctgcac
    37261 aatatacagg cagatttcat aaaacagtaa ctgtatgttg gaatacgaaa gaagctttaa
    37321 gtatctttaa tatatccggc gaagtgagaa cacttcctga taagttgttt atttatgata
    37381 gtgttcatca taaatatgcc atctttgaca gtatagatta tagggtcata aagtttatgg
    37441 gtcaaaattg ttatactatt acgaacatgg attctatttc acaccaattt tcaattgata
    37501 aataccatgg attagaatat gtgaattcag gttggatttt tccgtcagga tatacttgtt
    37561 attttcatca aaatgagcat attctctatt ctagtgagct tcgttccggc aagagtatta
    37621 atttacattt tataagaaaa gaagagttca ttcatgagat tgaattatta atggatggaa
    37681 catattatat tagaataaag attgactact aagcgcatgg ttcaaatttt tgtaattttg
    37741 caacatgtat tcccttattc gtttcttttt gtttaaaatg gatgctgaaa aagcccatca
    37801 ccttactttg aaaataatgg catttgcaat taaaattctg ggaataagaa tgctgttgaa
    37861 taagttattt gcattagatg acaaacgcct ggaacgtact gtttttggca ttacgtttaa
    37921 aaaccctgta ggattggctg ccggactgga taaaaatgcg caatgtattg atgctctttc
    37981 cgcactggga tttgggcata ttgaaatagg aacggttacc cctttagctc agccgggcaa
    38041 tgatcaacct cgtttgttcc gcattattcc cgatgaggct atcatcaaca gaatgggatt
    38101 taataatgaa ggtatacaga cagttggcag taatatcata agcagcaagc agttcaagca
    38161 gaaaaataaa gaaggaaatt cgagaactat tataggtggt aatatcggca aaaacaaaat
    38221 aacgcctaat gaaaatgctg tagatgatta tctgaaatgt tttgaggcac tttatgagta
    38281 tgtcgattat ttcgtggtga atgtaagctc gcccaatact cctgatttgc gggcgcttca
    38341 ggagaaagag cctttgaaaa aaatcttgtt ttctttgcaa gaaaaaaata atcagcaagt
    38401 aaaacgcaag cctgttttat taaaaattgc acctgatttg tcaaacgagc agttggacga
    38461 tatcttagaa gtagtagctg acacgaaaac agacggcatt attgccacta atactacttt
    38521 atctcgaacc gggttgagta tttctgctga tgaaattact gcaataggag caggtggatt
    38581 aagtggcaaa ccgcttacgc aaaaatctac agcaatcatt cgctatttag caaaaaaaac
    38641 aaaaggggaa ttacccatta ttgcagtagg tggcatcatg acggcacagg atgccctcga
    38701 taaacttgaa gccggagctt gtttggttca actttattct ggttttattt atgaaggccc
    38761 acgcctaatt aagcagataa acaaaaaaat cctgctttca aaataagcat tatttcattt
    38821 tccatctttt atttgggctt ggttttcgat tttgttgttt tctttggtgt cggtactgtt
    38881 gttgtagaat cttgagccgg agccggggtt tccggtactg cttccgggtt tacttctgta
    38941 gctggagttt ctgattcagg ttgctcttct ttcttaggag tcggtgcagg ctgtgaaagc
    39001 gggaatgctt ttttaggctt ctgtttgaat tcgtcttttt gttctttagg aggtgtcggc
    39061 tgttcaggtg ttagtgtgtt attcttaatg acttcagagc tgtctgaagg cgtaacaggt
    39121 gtaggctgtt caatattatt aggttgttcc tgttgaacgg gagcagatgg tgtcttcagt
    39181 ggtgcagata ctggtatttt tgacttgaat ctttctttaa aatcttccat gcgaatcaca
    39241 aaattatcgc gcatcattaa atctgctggc ataaactcaa agatggtcaa tttattttct
    39301 ctgaaaactt tatcggaaga ttttaatgcg gccaaggtat tcataacctc aggagctgaa
    39361 gaagcaatac ccatttgccc ttttttcact ttcataaaga gccaatttcc gcttttactt
    39421 tccagataaa tattgacaaa gtcgctctca taagcataac ctatttccac atatgcatta
    39481 taggttcgct taataatatc atttccaaaa atagcaaggc tcacgttgcc cacactcttg
    39541 aatgaagcat cttgcgcatc ccagaaaaac tgtgtgccag aaagcaacaa attataatca
    39601 aaacttttag gaataaagaa ggaatctgtg gtgtacatac ccgccaacag ttgattggct
    39661 tccaatgaat ctttagttaa aacagacatg gtgcgttgca ccgttttatt tcttttataa
    39721 gaattggcaa atgtaacatc tgtttcggca ttcaaataag catttatcaa ttgattggaa
    39781 ataacgggat tcattttaaa tttcaaagcc atatctgctg ccagtgttag ttcagttggt
    39841 ttagatggac taaatgtaaa actgcccgca gttgtaactg cacaaggata caagtctaat
    39901 cccaaaccaa tttcgccatt ggcatccatt tgtttagagg tttcattata ctttatacaa
    39961 gatgcatgtg aatagtattt tgtcaaagca ctttcatcac caaacagcat agtattgggt
    40021 tctgttttgc tgttcgacat agtgcttctg acattcataa ttgcagcgtc acccggtgaa
    40081 tgtttatctt gcaaaatagc ctgataaaac tcaatattcg ttttatccaa taataatcct
    40141 acaaaaatat tattattgtc tttcaatact gattctaagt tgatagctgg cttgttcgga
    40201 tccagttctt gtttattcgg aatccagtcg gagctggtgc ctgcaatttc aattttgata
    40261 tcgccgtcca agaatatatt tttatgttta gagtcaaagt taaagttgcc cttataaaac
    40321 actttattat ccagcttaaa gtttgccgct tcttcaatta ttgtattcgc atacgtaaat
    40381 actttactgt agtctcggtc tgaaagtgtc tttttccctt ttttcccttc cggaattact
    40441 tgtcctttat acggattatt tacgccaatt tctgccatag acaccttcgt agatgatgct
    40501 ccgtttttgt agtagtaatt accagttccg gaaaaatcat ttcttcccac tacattaatt
    40561 tctgcatctt ttaccgtatg ataactactg tcacggttaa aaattacaat tgcatttttc
    40621 aatttacgaa ccgtaccgtc ttgttcaata aatatttctc ctttatccgg aatcactttt
    40681 gaatccgcca gcaataattc attgatacct gcaaaatgta tggagttgtc attaagattc
    40741 aactcggcag tattcgtaac aaattttaat gaatctttag ttggatcggt agaaagcagg
    40801 aagaatttag cacttgtaga tgctgcattt aatgtcagca tattttttgc caaatcgaaa
    40861 tgcagatttt taggattgga aacatattta aacgatgcta aaggaatcgt atcattttta
    40921 attaactcca cgtctgcgat tttcttatcc aagtcataaa gtgcattaac tgcatccgat
    40981 gccaataatg aatttccttc ttcggtaacc aaattcagtt tagcattctt agctgaaaat
    41041 tgtgtagcac cgaaggcaat ttcatctgat tccagtttgg ttttaccgat agacaacaat
    41101 cccttaccca ataaatcttt tttgtacaaa atcattttac cattcagatc tactacatta
    41161 tcatagaagg taaacttttc atctttagca ggtgatacaa ccaatgagtc aattttttga
    41221 ttccaaacaa aagatgatgc atttattttg ccttcgggtt tattggcttt tttatcttga
    41281 gtaatgcgaa tgctgtctaa tttcgcatat acactgtcca taaaaaagaa ggaggtatcg
    41341 gaatacattt ttatcgagcc aaattcaaat aaccctttcc cactcaagcc atctgcgttg
    41401 agttttaaat ctgaaaaata ttttccttta ttgccaaata agtcatatcc gcctttaggc
    41461 gtttctattt gcaaaccaaa tgatttatca tcttgtaatg ttaagccgga ttcgatagga
    41521 ttgaaaattc ctcctgaaat caatcgccct ttaaaatcta agttttcggt taagacatca
    41581 ttcaaactgt caatctcgaa cggataaatc tgataataga atttatcctt attgtattta
    41641 tcgccattcg ccccccggtc ataagtaact ttagacgtat ccccactgga gaagaatggg
    41701 tatttcaggt tattgtttgt acctgattta ttctcaggtt ctgcaatatg caatgtgcct
    41761 gaaatatttt gtataggcga gcgtgaaggg gaaagaacgt attgcccttt ttcgtttggc
    41821 ttatcatttg gcacatatac caccattgag tctattttat tcatattgaa cgcatagttt
    41881 tcataatcga aatctacttt ctttgccaag aaatcaaatt ttccggctat aatacgtcct
    41941 cccattgtca tggtcctgtt tttgccaatt ttaactgtat cagtcgaagg gataaacttg
    42001 acacccgatt tagaactcat attgatttca tctacaccat agacctccag aatattactt
    42061 tttatgtcca gtttagcatt tccatatttt ttaaaagaag caaatttgat aaaatcataa
    42121 tcctgtttgc cttttgcaga tagagcctga ttgattaatt tatcttttac ttcaatcgtt
    42181 ctggttgatg catcatattt gatatagcca tcttccatta atttgacgaa agtaggctgt
    42241 actgcattat aaggtgcatt ggggctaata tcaaatgcta ccgactgggc gtctaatttt
    42301 cgttcatttg caactgcata gaaagcataa attcttgaaa tgggaccttg ttcgtcagag
    42361 cccttgtatt tgttctcaat attttgctga tagtaattat acgattcgaa tatcgcaggt
    42421 ttttcaccac taagtgttgc cattccaaat ttcatcgtca agctgtcaat attccagctt
    42481 agttcatctg catagatgaa catttgatga taatcagagg taaatggctg tttagaaagt
    42541 ggtttcatat cacgataagc cattaaattc tttgtttttg agctataatt gatgttcagg
    42601 taattgtgag taatagtatt atcttttaac agaatggcca atgatgcatc tgtgagtgat
    42661 aaatctttaa aatccttaat ggcaatgcgt ttgcaagttg cttttaccac ctctttgttt
    42721 tctttatttt ttagggttac taccggctga gaggtatcgc tgccaaccac ataaatattg
    42781 gttccttcca gttgaaatcc acctactaaa ccaatttctt tacttaacgt attaaagttt
    42841 acatctttct tgtatgatgt aaatttggga taaactcgct cctgtgattt tgcttgtaat
    42901 aatttatcaa ggtaagtacc caacaatact tcattcagaa ccggtttgaa tgtaaatttt
    42961 gcagaatcaa taacaatatc tgttttggaa aaattgattt ggtgatttcc gaaattcaag
    43021 aagttgttgg ctggatcaaa tccggcacgt tgcaaggaga tatttccttt atttcctatc
    43081 cacaaatttt ctaaaggata atatttcccg ctttctcctt taatatttat ggtgtcggct
    43141 gtagaaatac ccatcaaatc agttgataaa aatgaatata ccggcttatc atcttttatg
    43201 tcaacagaat aatcgccgga agcataccaa ttattggtat tggatgcgta cagtgtattt
    43261 tttatgtaca gattattcaa gaagttaaca taggtatcaa attctttcag gttaccccct
    43321 tttgagcggt cggctattgc ttgcagaata tcgaaatttt tgtctattgc cgctgtactt
    43381 gcttttgctg tttccaggtt gcctaacatc gttgcaaaat tgaacatgac cggataagcc
    43441 ggagcttttc tcattaataa agcctgagtg atatttttga tttttggaaa atatgtggat
    43501 gccttgcctg cggtgattac ttttggaaat ttttctgcca actctttaca gtcatctctt
    43561 gagcaatttg cttttataaa atcggagaat gattttgcat agccggcatt atcagccgga
    43621 aattctttcg ggtattgtgc aaatgccgaa aaacgaatca acaaaataag aacggtcagt
    43681 atgaatgagt ttctcaaagt aatgtaattt tattgtttac caaatataat acaatcccat
    43741 tgattgagcg tagttaaata gtgtgtgtac aaattgcatt ccaaagctgc atctaaaaca
    43801 aatttagcat ctgtatcata aaaacccgaa caaaacaagt agccgccact cttgagcaat
    43861 tcagcactat tttgcatatt ttctactaaa taatttctat gaatattagc caaaatgatg
    43921 tcaaactttt gttcgtctac tttttcaatt ccacctaaaa tattcttcgt tttttgtgtg
    43981 tgattgagtt tgcaattttc ttctgcattc gtaaaacacc attcgtcatt atctaccgca
    44041 accacagaac aagctcctag tttttcagct aaaatggcca gaatacccgt accgctgccc
    44101 aaatccagca ctgttttttc cttgaaatca attaagcgca tgttttgcat catttgcata
    44161 gtagtagcat gatgccctgt cccgaatgac attttaggat cgataataat ttcatgctct
    44221 acatcttgta ttgccggatg aaaagtagca cgaactgcca caaaatcatt gatgaccacc
    44281 ggctgaaaac cagcttccca ctgctcgttc cagtttttat tttctaacaa ctcaatcgtg
    44341 tattttactg aatatgtttc agctatatcc tttaagaaag attccatttc agcctgctga
    44401 ctttcattaa aatacacatc cacttcctgc tctttggctt caaatccttc acaatccaag
    44461 gtgtaaaaga ctccttcaaa ttcttctgca tctatatttt gaggtaattc aatttttgct
    44521 atataatttt tcataattgc cagtattatt atgtaaatgg gtttttgtat tacagcatta
    44581 gtgtgggctt tttaaacatc agcattattt cagcagcaac catttgctac ttatctcaca
    44641 ggagcattat ttcatggaac aaattatctc ggcaaactca tcggctttta gtgatgcgcc
    44701 gcctaccaaa cctccgtcaa tatctgcaca accaaaaaga tccattgcat ttttcccatt
    44761 cacacttcct ccgtacaaaa tactgatgga gtcagctact tctttggaat agcgcaatgc
    44821 cagctcttca cggataaaag catgcacttc ttgtgcctgt aatggcgtgg cagtttgtcc
    44881 ggttccaatg gcccatatag gctcataagc tatcaccaga tttttaattt cttcttcgtt
    44941 taattgaaat atacagttgt ctaattgtgc tttaataaac ttaaaatgtt catttgccat
    45001 tcttattccc aaaggctcgc cacagcaaaa aatgggaata atctcatttc gtttagcttg
    45061 ttttattttt tccagcaaca gcaaatcacc ttcattaaaa tattgtctgc gttcactatg
    45121 tcctataagc acatatttta cacaaacaga tgccagcata acagcactta cttcaccggt
    45181 aaatgcacct ttttcgtgtt cgctgcaatt ttgtgcggca acatacagat aaggataatc
    45241 ggcagatttg tctaatgcaa tattcaagaa tggaaatggc ggtgcaagca ccacttgttt
    45301 tttctcatgc aaaccaaaag tattgtaatt ttccgtaatg gtatctacta aggcttcagc
    45361 cgaagctttt tcggtgttca tcttccagtt tcctgcaatt attttttttc tcatggcaag
    45421 taattaagtg agtgctttaa cagttctttg ttcgtataaa tagattatta ctgctttatc
    45481 ttcatcactt agcagcagtc cccttctttc cataacacgt tgtgctactt caagagcttt
    45541 gtctaattga aaaatcgcat tttcgcccca tgcaaaatcg ggtatgtatt ttggtggaaa
    45601 atcgccactg aaaacattag caaaaactcc cgtaactgtt ccggtattga actgggtatt
    45661 tattgcagct ttggaatgat cgcccattat caagccacaa aactgcaatc cggtattgat
    45721 gaaatcttta gccggataat tataggcttt tactttcccg taattgttct tcaagttaga
    45781 attatttgtg tctgcaccga gattgcacca ttcacctaat actgagttac ccaaaaaacc
    45841 atcgtgggct ttattggtat agccagatat aaccgaattg gaaatctcgc ctcctatttt
    45901 gcaataagga ccaatagttg tgtcaccgta aatttttgcc tgcattttca atgtagcatg
    45961 ttcgcaaagc gcaaaaggtg cgcgtatggt acagccttcc atcacttcag catcattacc
    46021 caggtaaatg gggccatctg tactatttaa aatggcaaat tcacatttta caccatcttc
    46081 cacataaatt tcgcttccca acaactggtt agttgctgat aatttttccg gattcggttt
    46141 taatcctaac aatgcaacat catttctgat ttcatttcca ttgtgctgaa aaatatgcca
    46201 agggtactta atttgataat ttgataattt gataatttga caatgaactt cattatagtt
    46261 taatacttct cctatattaa gttttgcaac actgtgaatt gctatcactt gtccgtcttc
    46321 gctttgcaat tttgtatgct cattcagatt tttgatatca ttaagcaatg ttgcatcagg
    46381 caaaaagcgc gcattgatgt aaatggtatt ttcttttaga agacaagtat atttgggctg
    46441 taagtaagga actgttaata tttcaacagg agcatgcaga tatttttccc atttttcttt
    46501 tatggtcaaa atgccaatgc gcatatcggc aatagctcgg gtagcagata agggcaataa
    46561 gtgatcgcgt tgttcatcat caaacaagat aaaatgtgtt ttcggcatac tgtaaaaata
    46621 aaaaagtcgc aacagaaatg tgcgactaaa ttatttttta taagaaaaaa cggcttattt
    46681 tttctttgcg tattttttgt tgaatttatc gatacgacct gctgtgtcga taaatttcat
    46741 ttttcctgta taataaggat gcgacatgtt ggatatctcc aatttcacca acggatattc
    46801 gttaccgtct tcccatttaa tggtttcttt gcttggtgca caagattttg ccaaaaaggt
    46861 atgatcacaa gaaaaatctt tgaataccac taatctatat tcttttggat ggatgtccgc
    46921 tttcattttt tttaattttg gaatgcaaag atagtaattt ctgacaaata ttgcacatca
    46981 tctgctgtaa aattaaggaa ttaacaaaaa gaacataaaa cagacatttg tattaaatta
    47041 attgacaaat gtcgtgtgtt tcttattaac cttagttggg ttaggcgttt caattaaaaa
    47101 tagtagattt tttatataga accacctcct tttaattcct cctccttgtt aagttggagg
//
LOCUS       DAOQII010000002        27639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_758_length_27639_cov_5.363218, whole genome shotgun sequence.
ACCESSION   DAOQII010000002 DAOQII010000000
VERSION     DAOQII010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 27639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27639
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_27639_cov_5.363218"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1549
                     /locus_tag="PLF48_00200"
     CDS             269..1549
                     /locus_tag="PLF48_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF47768.1"
                     /translation="MEKYGQALLIAIPCFLVLILIEIIYGAIIRKDQLNNMDIISSLS
                     SGMTNILKDTLGLTIVVISYPFLLSKIAIFHGDKHPVLMAILAFVIMDFGGYWYHRFT
                     HTINYFWNLHIVHHSSEEYNLPCALRQQFSVFTNLPNIFFQGILMAVFGVPFEIYAII
                     APVHLFLQFWYHTRYIDKMGFLEYVLVTPSHHRVHHAMNDIYMDKNFSQIFIIWDKLF
                     GTFQVELDEIPPVYGVRRPVRTWNPFLINLRHLWLLITDTVRTKSIKDKIAVWFMPTG
                     WRPADVTEKYPVFYIKNMNELQKYNPVYSTAFKVWSNIQLVFVFFLLIYLFTQFGKLP
                     MNLVMQNGLFILLSIFAFTSLMDKSSIGLMAEWLRFLIGAYIIVSTGDWFGINAYISS
                     GSWFILGYLLIAAIVSSYFYFKEFKPENKTALAV"
     gene            complement(1671..2594)
                     /gene="miaA"
                     /locus_tag="PLF48_00205"
     CDS             complement(1671..2594)
                     /gene="miaA"
                     /locus_tag="PLF48_00205"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNF47769.1"
                     /translation="MHNNKHIIIIGGPTAVGKTALAIDLAKAYNTEIISADSRQIYKE
                     LAIGVARPAQEELALVPHHCIAHKSIRDYYSSGEFEKEALQILSEQFLQKDTVVVCGG
                     SGFYIQALCDGMDEMPEVDEQIRTQLNHRFQAEGLQTLQTELKLLDEETFLSIDIFNT
                     QRVIRALEVCLSTGKKFSSFKQKKKIQRPFGSIKIALNLPKEKLHERINKRVDWMMEN
                     DLLEEAKAVLPFANNNALRTVGYQELFDYFAGKTSLNEAVDLIKMHTRQFAKRQLTWF
                     RRDKNFVWFAPDERDKMETYIQQQLNRQSSD"
     gene            complement(2643..4076)
                     /locus_tag="PLF48_00210"
     CDS             complement(2643..4076)
                     /locus_tag="PLF48_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF47770.1"
                     /translation="MQFQFSDIELEILQTVAKAAKELDVQAYSVGGFVRDKLLHRPCK
                     DIDIVCVGSGIDLAHKTADIFYREHHVKIKVAFFKNFGTAQLKWKDVEVEFVGARKES
                     YQRDSRNPIVEDGTLEDDQNRRDFTINALAIHINNDNFGELIGPFNGIQDLENKIIRT
                     PLAPHQTFNDDPLRMLRAIRFASQLQFTIEPETLAAIEPLADRLQIISMERIHVELNK
                     IILSKTPSIGFKLLFQTKLLHRFFPEMVKLQGVSIQENYGHKDNFYHTLKVLDNISEK
                     TDDLYLRWAAILHDIAKPRTQRFEEGHGWTFHGHEVVGARMVPKIFTKLKMPLDDRMK
                     FVQKMVLLHLRPISLTKENITDSAIRRIIFEAGDDLESLLLLCEADITSKNETKVKRY
                     KENLKLVQQRIKEVEERDHIRNWQPPITGDIIMQAFAIKPSREVGVIKDSIKDAILDG
                     IIPNDYDSAFQLMLQKGNELGLTVFKG"
     gene            complement(4163..4885)
                     /gene="recO"
                     /locus_tag="PLF48_00215"
     CDS             complement(4163..4885)
                     /gene="recO"
                     /locus_tag="PLF48_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNF47771.1"
                     /translation="MLEKTEGIVLKTTRFGETSVIAKIFTKKYGLISFMLKGLRSQKA
                     PKTASLIQPLQMLQLDISVKDNRNLQHIKEYRSDYIYRSLHLDFSRQAVAVFCQEVLL
                     KCLKEHEINERVYDYFISFLLELDQTAVRFENQSLFFLLELSGLLGFEPSLTHISDYA
                     YFNLASGKFEQQLSSTEPCLDTTETMLFKRLLAEYYEKNDTPFTGAERKILLDKLLLY
                     FRWHVPEFTQPQSPAILHEVLK"
     gene            4983..5204
                     /locus_tag="PLF48_00220"
     CDS             4983..5204
                     /locus_tag="PLF48_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47772.1"
                     /translation="MKNFRKGFLFSLIVVSIATTSCKKVWYCDCKSGGKTVTSTAIDN
                     VGKVGAKSVCWEYEYQAHVNGANYECNIR"
     gene            complement(5210..6499)
                     /locus_tag="PLF48_00225"
     CDS             complement(5210..6499)
                     /locus_tag="PLF48_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNF47773.1"
                     /translation="MKYLPISSDLFIENRKRFTAQLAPNSMAIFFSNDEMPRSADQNF
                     AFRQNPDLFWLTGIDQEETILILYPDCPNPIYREALFLRKTNEVIAVWEGHKYTMEEA
                     KAASGIKEIFWNSAFSTALPALMAYCEKVYLNFNENDRAVNHVPYKDIRFAHELKFNY
                     PGHEIKRSGPILSALRSVKLKTEIELIRKACEITRDAFLRVLKTIRPGMMEYDIEAEI
                     IHEFIRQRANGHAYSPIIASGKNACVLHYVENNKTCQDGDLILMDFGSEYANYASDLT
                     RTIPVNGKFTQRQKDVYNAVLRVMKQAKTMLKPGAILADYNKEVGKIMEGELIGLGLL
                     NKDDVAKQSEENPLYKKYFMHGTSHFMGIDVHDIGNRYAPMEVGNVFTCEPGIYIPEE
                     NIGIRIENDILITENGIMDLMHDIPLEVEEIEELMRR"
     gene            7103..8905
                     /gene="typA"
                     /locus_tag="PLF48_00230"
     CDS             7103..8905
                     /gene="typA"
                     /locus_tag="PLF48_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNF47774.1"
                     /translation="MQNQQIRNVAIIAHVDHGKTTLVDKILWECKTFDEHENKGVLIL
                     DNNELERERGITIVSKNAAVKYKDFKINIIDTPGHADFGGEVERVLKMADGVLLLVDA
                     FEGPMPQTRFVLQKALQLGLKPIVVINKVDKENCTPEEVHEKVFDLMFHLDATEDQLD
                     FPTLYGSAKNGWMNTDWKVKNDNIVPLMDAIVAHIPAPKIAEGATQMQVTSLDYSSFV
                     GRIAIGRVFRGSIKANQNVALIKRDGKITKSRVKELMLFEGMSKVKVDEVHAGEICAV
                     VGIEGFEIGDTIADAENPQAMAAISIDEPTMSMLFTINDSPFFGKEGKFVTSRHIKDR
                     LDKELEKNLALRVEQGDTADAFMVYGRGVLHLSVLIETMRREGYELQVGQPQVLFKTI
                     NDQKHEPYEELTIDLPETKSGTATEMVAKRKGDLKNMILKGTRTLLEFDIPSRGIIGL
                     RTELITATSGEAIMSHRFTRYDVFKGEIPGRIAGSMISKETGEAIAYSLDKLQDRGKF
                     FIEPGDEIYTGMVVGENSRPDDLVINCTVTKKLTNVRASGSDDKVKLAPPIKFSLEEA
                     LEYIQSDEYVEVTPKSIRLRKIYLDENDRKRYGK"
     gene            9002..9472
                     /locus_tag="PLF48_00235"
     CDS             9002..9472
                     /locus_tag="PLF48_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47775.1"
                     /translation="MNKFLFAVLFSILFLNACVPIGNNVNITINGNVENNSDYCGGAA
                     PPQELLDQLAMYHPSAMQKFYVRQGSVAMPFTPVYTFFTTDSLGNYTISLPAGQYTVI
                     SEEKYLTESNAAIDSSCTYLHTPDFSLNIQASQTAYTNSYTITCNYTCTGQMPQ"
     gene            9773..10347
                     /locus_tag="PLF48_00240"
                     /pseudo
     CDS             9773..10347
                     /locus_tag="PLF48_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            10441..11376
                     /locus_tag="PLF48_00245"
     CDS             10441..11376
                     /locus_tag="PLF48_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47776.1"
                     /translation="MRKILVLLAIGIGTFFNTSALDIDGKWQGTFIEYIHNTYDVQMI
                     MKVLPYNTFHANLKITNGQYFGEYNVSGNICSNRSLEITAIFLIRENSANNWIDCLNG
                     VWDLNEDENELTFTDTWLNQNSKKDVCKVKFANKDMFQCLRSAYFRRSTLYDFNPDFD
                     STWAQYTNKNKKQIIDETQLAKKEDKATPTLDTLTRINDREINVKESIEVSGDSVIIE
                     YWDRFNEDGDSITLYLNNKPIVYKKLLTKVKQSLTVKLTQKTNYLVLHAENLGKEPPN
                     TATIAIKDGKKIQTISLNSTLRTSSAIKLVVKDKK"
     gene            11517..12440
                     /locus_tag="PLF48_00250"
     CDS             11517..12440
                     /locus_tag="PLF48_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNF47777.1"
                     /translation="MEVTIWTWAGFIAFVLLMLALDLGIFNRKHKEISIRSALLWSLV
                     WIGLAMIFNLGIYAFLGKAKAVEFLTGYVIEKSLSIDNIFVFIMVFGYFNIPSKYQHK
                     VLFWGIIGALLMRAVFIFAGVALITRFHWIIYIFGAFLIFTGIKMLTQEDKALEPDKN
                     PLVRLFKKFMPVTDKLYEENFFVRINHKNYATPLFVVLLLIEFTDLIFAVDSIPAILA
                     ITSDSFIVFTSNVFAILGLRSLYFALAGITKYFRFLKYGLSVILMFVGIKMCIVHWYK
                     LPTLLSLGIIVSILLISVLASVIIKPKDNTV"
     gene            complement(12458..14581)
                     /locus_tag="PLF48_00255"
     CDS             complement(12458..14581)
                     /locus_tag="PLF48_00255"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNF47778.1"
                     /translation="MSITITKNVSPDAAKDIFELQQKINAFVNGEIPEDKFKHFRLTR
                     GVYGQRQLGVQMFRIKIPMGRITPAQLVRCAELSELYGSSNLHLTTRQDIQLHYVKVD
                     NAPLVWAGLEDCGLTGREACGNTVRNITASASAGIDKNEPFDVSPYVYALFKYLLRNP
                     ICQDMGRKIKIAFSSSEKDTAFTYIHDFGFIPKIENGIRGFKVVVGGGLGAQAILAQT
                     AFEFLEDDKIIPFVEAGLRVFDRYGERTRRHKARMKYLVKELGVDQFLTLIQEEMKAN
                     AHLVFPVETDGFESPRSPEIKQVPTVDIKDKKRYEDWLETNVFEQKQPGFYGVYLKVT
                     KGDIKPDQARVIAQLANDFASDDMRITMNQGMLLKYVRKEALPKVYSVLDSMGFANPG
                     FGTIADITTCPGTDTCNLGVTNSMLLAEILEDVIQKEYYDLIFDKAIHIKMSGCMNSC
                     GQHMSADIGLHGSSIRVGDKVAPAMQICLGGGVDNNGVGMAGDKVVKLPTKKMPEALR
                     IILDDYENHSDETEYFNNYYRRQGKNYFYTLLKPIADTSKFVDDDFYDYGHDELFTPE
                     VGVGECAGVFYDMVSTILKDAQLKQTEALQALDNSKYAEAIYYAYTGFVTSAKSLLLS
                     KEVECNTQIKILQDFDRVFVVTGEITCEWGFEKLVLSINKHEPEREFAQSYVGAYEQF
                     LNSALDYRNATMKADKLVKESDYKA"
     gene            14814..15653
                     /locus_tag="PLF48_00260"
     CDS             14814..15653
                     /locus_tag="PLF48_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47779.1"
                     /translation="MKYIIPLFICIIVGQSCQKNENEGSPKEPGPPQLAAYTDSMLHA
                     VGLQSYSDVLTGKLEGNFEIFEYCTPYDVNCSDFTPQTELNGSVSLDTNRTSTIDAGV
                     LKINQLSITPNTNKRYEIHFGTSQYNSQQAQLNLIYGATNTIKLVKNGVDVFNKSLYV
                     PKHIKYTGYDCATSKMKNDPIKANDEIYWNSDYSNKNGILIRFTGNDSRGIEYTFYRI
                     VSDLGKYAVTESDLSYFPKEKNGMGIQVELNRGNFFLTYGTDGRRYNVTINASCKYYF
                     NLF"
     gene            complement(15721..16269)
                     /locus_tag="PLF48_00265"
     CDS             complement(15721..16269)
                     /locus_tag="PLF48_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47780.1"
                     /translation="MKKLLVTLAICTGLSAYANDSLTVANNSQDVNENDYAERKISAI
                     TETGMNYIYGKPFFSIYQAVGAKLNPYVFIGEGVGIQIRNTNQWQFQLLTDLRVHALN
                     KWVTPTFTIQAGLNKTGKAALTNEKTQKQLGENSMALNLGTGVLFRVKENASFSLTGG
                     YNLNTDFKTNFNGGFVRFAYQF"
     gene            complement(16641..17849)
                     /locus_tag="PLF48_00270"
     CDS             complement(16641..17849)
                     /locus_tag="PLF48_00270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF47781.1"
                     /translation="MLIWLLISLMLTVAYVGIMWTYKNEWEEIKETHDLREECTTRVS
                     VIVPARNEEKYIGNILKDLLDQIYPASLVEIIVIDDFSEDNTFSIAASYSKANTTIKV
                     LQLKDSLDKNTITEAYKKRAIEYGIAQANGTLIITTDADCRLQKHWLHSIVSFYEKEK
                     CALIASPVGFYNDHSKFQHFQSLDFCGMQAITGASLNMKIFNMANGANLAFEKKAFDE
                     VGGYKNIDKNASGDDMLLVYKIAQKFPDKIRFLKNKAAIVLTHPAENVQDFLQQRFRW
                     TSKAGHYQDKRISFILLLVYLFVWSIWLNFFFVFGKIAYYRIFPNPTISALFEFLNVF
                     LIFIILFIQILCKSLIDYTFLKSATNFFNRHDLMQSFAYSQLLHIWYIPFVGTLGLVF
                     PYRWKGRKLK"
     gene            complement(17849..18304)
                     /locus_tag="PLF48_00275"
     CDS             complement(17849..18304)
                     /locus_tag="PLF48_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF47782.1"
                     /translation="MTVQFKAKIVKDEKMDVFYIPLELDVEKTFGKKRFKAKIWYNDV
                     LYRGLIAKYDGGYFLMINKAIRAQLNKKAGDIVNVKIEQDLEERIVELPTVLVDFFNR
                     ENLLKSVFDKLSYTHQKEYVQWITSAKREETLQNRMIKLKEMLEEKLKK"
     gene            complement(18324..19319)
                     /gene="dusB"
                     /locus_tag="PLF48_00280"
     CDS             complement(18324..19319)
                     /gene="dusB"
                     /locus_tag="PLF48_00280"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNF47783.1"
                     /translation="MVTIGNIQLGDFPFLLAPMEDVSDPPFRYVCKQYGADLMYTEFI
                     SSEGLIRHAKKSLQKLDIFDFEKPIGIQMFGGEEDAMVESARIIEQAKPDLIDINYGC
                     PVSKVVCKMAGAGILQDIPKMVRLTAQVVKATKLPVTVKTRLGWDENSKNIVEVAERL
                     QDIGIQALSIHGRTRKQMYKGEADWTLIGEVKNNPRMHIPIFGNGDIDTPQKAIEYKN
                     RYGVDGIMIGRAAIGAPWIFQQMKGFLFENKLIPEPSIEERIQVIKTHLQKSIEWKGE
                     VLGILEMRQHYSNYLKGLPNIKLFRAKLVTTNQRENLFTILDEILQTYQLIHNIE"
     gene            19406..20341
                     /locus_tag="PLF48_00285"
     CDS             19406..20341
                     /locus_tag="PLF48_00285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNF47784.1"
                     /translation="MSETILDDLQLGQNKLLYGINQKDGFTQFLILMGIFVACFIAGQ
                     LLSAGLLYLSYGLDVADMSTLMKKHKDLNAFRYAQMLATVFSFLAPAIIFSKQKQKNW
                     LQYNRADKSFNLVLIALIPLLIISFYPLINVSFFLNKSLGISNWFQGMQGDYKEIVLA
                     LTAGTSVSTLILNLITVAVLPAICEEWFFRGTMQRFFSEKMNIHLAIFLSSIAFSLIH
                     LEFSGFLPRIFLGMLLGYIYYYSGSLWASIFAHLINNGAQVVAIYLSNKGLYTVNMDN
                     PDFPTYRELLIFTPLFIALFFGMIYLSKQKRSTFA"
     gene            20369..21223
                     /locus_tag="PLF48_00290"
     CDS             20369..21223
                     /locus_tag="PLF48_00290"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNF47785.1"
                     /translation="MNDIGTRIATGIIFLIVMIGSVIWNQYSFGILFGLIVVLCISEY
                     QTIIDGLLVATPRWKTLYKYINLIFGVITFVIVYLVAAKKLPLMYMSFVCGFPLTWLV
                     IEMYTKSERPFYNVVLNSMPIYYLAIPLSSSSLLAFHDGGYNWHYLLAIMIFAWSNDS
                     FAYLAGRFFGKHKLFERISPKKTWEGFFGGMAGSVLFAYLVFLLFPKWTHQPVSLTTY
                     IALAIITTITSTYGDLAESMIKRSLQIKDSGSALPGHGGFLDRFDGLLFSLPACTFYL
                     TLFLPSPV"
     gene            complement(21206..21712)
                     /locus_tag="PLF48_00295"
     CDS             complement(21206..21712)
                     /locus_tag="PLF48_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47786.1"
                     /translation="MSINMGIAQEYKGAIGLRFGYGWGLSGKYFFNSNGYGKSSGHAI
                     EGLLRYGYHGVVFTKPGINISALYEKHWMLSRDERWSFLLGGGLSLGYGNKSTTNIFA
                     FGIDPILGVDVLLRKFPINFTFDYKPTIYFDKYIRNNSIKVKPSVSYYEIALSVRYAF
                     TGKSYRRR"
     gene            complement(21825..23150)
                     /locus_tag="PLF48_00300"
     CDS             complement(21825..23150)
                     /locus_tag="PLF48_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF47787.1"
                     /translation="MNKISSFIIACLLFFTASAQTKSVQFSAALENKYAISSDSADVY
                     LYLKVTGNELKSDKKRAPLNLSIVLDRSGSMHGEKLEYAKKAAAFVVEQLNADDIVSI
                     VDYDSDVEVVSASDFVKNKQVLTEKINKILDRGSTNLSGGLLEGYKQVLSTKKEGFVN
                     RVLLLSDGLANVGITDTAKLKDLVQQKFKETGISVSTFGLGEGFNEDLMTAMAENGGG
                     NYYYIDSPDKIPGIFAKELDGLLSVVAQQAKISVSYASEFLIPEKTYGYNHKMENMRL
                     LISLNDVYSKEEKNILVKFKIKKPFVQDLVFTSTLNYIDAQSYNTENIVVVNTLHLTR
                     DTILIKQNLDSTVSEMVVMFEATEMMDDIMRDIDQGNYTIAASKMEKVSGFVNANQSK
                     DKTGKINKVKSNVDKYKEDIKDIDKKNVYEKSKIQKSTKSNNYNIKKSK"
     gene            complement(23161..23571)
                     /locus_tag="PLF48_00305"
     CDS             complement(23161..23571)
                     /locus_tag="PLF48_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47788.1"
                     /translation="MTVPASILDKDKKVNSNNIGVDIVVEDCLKQTAGDLQFTFTTCS
                     SNPHELRNTHKANCIGYAQYFTYLFYKYDSSIWQAKPAIGEIKMCKINLNKLIKHPFF
                     KEHDFVIISNLKTGEKFAIDPVVYDYFHIKSINF"
     gene            23864..25459
                     /gene="nadB"
                     /locus_tag="PLF48_00310"
     CDS             23864..25459
                     /gene="nadB"
                     /locus_tag="PLF48_00310"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNF47789.1"
                     /translation="MRDILVIGSGIAGLSFALKTAQRFPDKKILILTKANEDECNTKY
                     AQGGIAVVTDFLKDNFEKHIADTLIAGDGLCDKEVVELVVREGRERVNELIAWGTNFD
                     KDEDGDYKLGREGGHSEFRVLHYKDVTGKEIERALLEKIHQQKNVEISQHHFVIDLLT
                     QHHLGSIITRVTPNIRCYGVYVLNRLTNQIEKIESKITLLATGGFGQVYRATTNPIIA
                     TGDGVAMVYRAKGRVNNMEFVQFHPTALFEPNVSPNFLITEAVRGDGGILKRKNGEEF
                     MQYYDDRLSLAPRDIVARAIDNEMKKSGEDCMYLDCRHMDKENFLHHFPNIYEKCKSI
                     GIDVFKDMIPVVPAAHYACGGIDVDEYGRTSINNLYACGECSHTGLHGANRLASNSLL
                     EAVVYAHRIFEDVAEKIDAISYPTEAIPEWNAAGTSDPKEMVIITQSIRELKDIMSNY
                     VGIVRNNVRLKRAQERLYLLFKETEELYETTTISPQLLELRNMITIGYLITRSALLRK
                     ESRGLHFTTDYPTHYPHVEETLL"
     gene            26072..27592
                     /locus_tag="PLF48_00315"
     CDS             26072..27592
                     /locus_tag="PLF48_00315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNF47790.1"
                     /translation="MSQVDLPKIIAHCKEYGFIFPSSEIYDNLGAVYDYGPFGAELKN
                     HIKEYWWKSMVQMHENIVGIDAAIFMHPTTWKASGHVDSFSDPLIDNKDSKKRYRADV
                     LLEEKIEKLREKGEKELTKKNLSDASVEEKAKASRDYAKAITLEAEYIKAAEAADFAR
                     YKALIEEYEIVCEVSGSKNWTDVRQFNLMFDTKLGSVADDANTIYLRPETAQGIFVNF
                     LNVQKSARMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFIKPGTQKEWFDY
                     WKKARMKWHLALGTAQENLRYHVHEKLAHYADAAEDIQFKFPIGFKELEGIHSRTDYD
                     LGRHQEYSGKKMQYFDSELNQNYIPYVIETSIGLDRMFLRVLSDGLKEEKLENGETRV
                     VLNIPPALSPIKVAVLPLVKKDGLPEKAREIMDTLRFVGRTFYEEKDSIGKRYRRMDA
                     IGTPYCITVDHQTLEDNTVTIRERDTMTQERVAIDKLESIVREKTDMNVLLRKAMA"
BASE COUNT         8438 a         5138 c         5033 g         9030 t
ORIGIN      
        1 ctgtgtgtgg ggggggggga tagtgtattc atgttttttt tatttctaca aatataataa
       61 tattattcca aaaacgcaat acctgtttat aatatttttt attttatcct ctattaagaa
      121 tactaattct attgttttca ataagttagc atttttaaca cctcgtaggt ggcaattact
      181 tcacttatct ttagattgaa aattagttgt ctttagttaa tcttagtaag tttactttgg
      241 ttgtattttt gtaaaaaaat accttgatat ggaaaaatat ggtcaggcac tattgattgc
      301 aattccttgc tttttggtgt tgattttaat agagattatt tatggagcaa ttattcgcaa
      361 agaccaactt aataatatgg acattatctc gagcttaagt tcgggaatga ctaatatttt
      421 gaaagatact ttggggctta ctatagtcgt gatttcttac ccttttttat tgagtaaaat
      481 tgcgattttt catggcgata agcatccggt tttaatggcc attcttgcct ttgtcattat
      541 ggatttcggt ggttactggt atcatcgttt tacgcatacc atcaattatt tctggaactt
      601 gcatatcgtt catcacagca gcgaagaata taacctgcct tgcgccttgc gccagcaatt
      661 ttccgtattt accaatctgc cgaatatctt ttttcagggt atactgatgg ctgtttttgg
      721 cgttcctttc gagatttatg ccatcattgc tcctgttcat ttgtttttgc aattttggta
      781 tcatactcgc tatatcgata aaatgggatt tttggagtat gtgttggtta caccttcaca
      841 ccatagagtg catcatgcta tgaatgacat ttacatggat aaaaatttca gtcagatatt
      901 cattatctgg gataaacttt tcggcacatt tcaggttgag ctggatgaaa ttcctccggt
      961 ttacggcgtt cgtcgccctg tccgcacatg gaatccgttt ttaataaatc tgcgccatct
     1021 ctggctattg ataacagata cggtacgaac aaaatccatc aaagataaaa tcgcagtttg
     1081 gtttatgccc accggttggc gtcctgcaga tgtaacagaa aaatatcctg ttttttacat
     1141 taaaaatatg aacgaattac aaaagtataa tcccgtttat tctacagcgt tcaaagtgtg
     1201 gtcaaatatt cagttagtct ttgtcttctt tttgctgatt tatttattta ctcagtttgg
     1261 caaattgccg atgaacttag tcatgcaaaa tggcttattt attctcttgt ccatatttgc
     1321 ctttacttca ttaatggaca aaagcagcat tggcttaatg gcagaatggt tgcgttttct
     1381 tatcggagcg tatatcatag taagtacagg tgattggttt gggataaatg cctatatttc
     1441 ttcaggtagt tggttcatac ttggctattt gctaattgca gccatagtgt caagttactt
     1501 ttattttaaa gaattcaagc cggaaaacaa aacggcttta gcggtttaac ctgatttcgg
     1561 gataaaaata gttgattttt ttttatcatt aacctcctcc ttttaattcc tcctccttgt
     1621 taagttggag gaaaaaagga gaaatcaact atttcaccac cttgttttta ttaatccgaa
     1681 ctctgtctgt ttaattgttg ttggatatac gtttccattt tatcacgttc atccggtgca
     1741 aaccaaacaa aatttttatc tcttctgaac catgttaact gccgtttggc aaactgtcgg
     1801 gtatgcattt taatgagatc tactgcttca tttaagcttg tttttcctgc aaaataatca
     1861 aacaattcct gatagcctac tgtacgcagt gcgttgttgt ttgcaaacgg aagtactgct
     1921 ttcgcttctt ccagcaagtc gttttccatc atccaatcta ctcgtttatt aattcgttcg
     1981 tgcaattttt ctttaggtaa attaagtgcg atttttatgc tgccaaatgg tctttgtatt
     2041 ttcttttttt gtttgaatga tgagaatttt ttgcccgtag aaagacacac ttccaatgct
     2101 cggataactc gttgtgtatt gaaaatatca atggaaagaa aagtttcttc atccagcagt
     2161 tttaattcag tttgcaaagt ctgtaggccc tctgcctgaa aacgatgatt gagttgagta
     2221 cggatttgtt catctacttc gggcatttcg tccataccat cacaaagtgc ctgaatataa
     2281 aatccactgc cgccacaaac aacaactgta tctttttgta gaaactgttc tgataaaatt
     2341 tgtaaagctt ctttttcaaa ttctcctgat gaataataat cccggataga tttatgggca
     2401 atacaatgat ggggaacaag tgctaattct tcctgagccg gacgtgccac accaatagct
     2461 aattctttat aaatctgtct ggaatccgcc gaaataattt ctgtattata agcttttgcc
     2521 aaatcaatag ctaatgcagt tttgcctaca gcagtaggcc ctccgatgat gatgatatgt
     2581 ttattattgt gcaattgctg taatgcttta atccgattat tttgaattag tgaagcagga
     2641 aattatccct taaaaacagt caaacccagt tcgtttcctt tttgcagcat gagctgaaat
     2701 gccgagtcgt aatcgtttgg aataatgcca tccaaaatag catcttttat actgtcttta
     2761 attacgccca cttctctcga aggttttatc gcaaatgctt gcataataat atcaccggtg
     2821 ataggtggct gccagttgcg gatatgatcg cgttcttcca cttctttgat gcgctgttgc
     2881 actaatttca gattctcttt atatcgtttt actttggttt cattctttga ggtgatatca
     2941 gcttcgcata ataaaagcag actttccaaa tcatcgccgg cttcaaaaat gatgcgacga
     3001 atggccgagt cggtaatatt ttctttagta agcgatatcg gacgcagatg cagcaacacc
     3061 attttttgta caaatttcat cctgtcgtcc aaaggcattt ttaattttgt gaaaatttta
     3121 ggcaccatgc gtgcacccac cacttcatgc ccatgaaaag tccagccatg tccttcttca
     3181 aatcgttgtg tacgaggctt ggcaatatca tgcaaaatag ctgcccagcg caaatataaa
     3241 tcgtccgtct tttccgagat attgtcgagt actttcaggg tgtgataaaa attgtcttta
     3301 tgcccgtaat tttcctgtat gctcacaccc tgtaatttta ccatttccgg aaaaaaacga
     3361 tgcaataatt tagtttgaaa aagcaatttg aaacctatag atggagtttt gcttaaaata
     3421 attttattca gttcaacatg aattctttcc atcgagatga tttgcagtcg atctgccaaa
     3481 ggctcaatag ctgctaatgt ttccggctca atcgtaaact gtaattgcga tgcaaaacga
     3541 atggcacgca acattcttaa gggatcatca ttaaatgtct gatgaggcgc taagggtgtg
     3601 cgtattattt tgttttctaa atcctgaatg ccattaaatg gacctatcaa ttcaccaaaa
     3661 ttgtcattat tgatatggat ggctaaggca tttatggtaa aatctctgcg gttttgatca
     3721 tcctccagcg tgccgtcttc cacaatcggg ttgcgactgt ctcgctgata tgattctttg
     3781 cgcgcaccta caaactccac ttccacatct ttccatttca gttgcgcggt accaaaattt
     3841 ttaaagaatg ccacttttat tttcacatga tgttcccgat aaaaaatgtc cgctgttttg
     3901 tgagccaaat caatgccgct acctacgcag acaatatcga tatctttgca gggacgatgt
     3961 aataatttat cacgcacaaa accgcccaca ctatatgcct gcacatccag ctcctttgct
     4021 gcttttgcta cggtttgtaa aatttcaagc tctatatcac tgaattggaa ttgcatgggg
     4081 caaagatagt aattcggtat gcaaagtttt ttgtataaat cacaggcagc atattaattc
     4141 agaaaggaac ttttaaacaa cattatttaa gtacctcatg taaaattgct ggagactgcg
     4201 gctgagtaaa ttccggtaca tgccatcgaa aataaagcag caatttatcg agtaatattt
     4261 ttcgttctgc acccgtaaaa ggagtatcat ttttctcata gtattctgcc agcagtcttt
     4321 tgaaaagcat agtttctgtt gtgtctaaac aaggttcggt agaagacagt tgctgttcaa
     4381 atttaccgga tgccagatta aaataagcat aatcagaaat gtgtgttaaa gaaggctcaa
     4441 agccaagcaa cccggatagt tctaacaaga aaaataaact ttggttttca aagcgtacag
     4501 ctgtctgatc taattctaaa agaaatgaga taaaatagtc ataaacacgt tcatttattt
     4561 cgtgttcttt caggcatttt aacaagactt cctggcaaaa aacggccact gcctgtcgac
     4621 tgaaatccag atgtaaactg cgataaatat aatcactgcg atattctttt atatgttgta
     4681 agttacggtt gtcttttaca gaaatgtcta attgcagcat ctgcaagggc tgtatcaaac
     4741 tggctgtttt aggtgctttc tgacttcgta agccttttag cataaatgaa attaacccat
     4801 atttttttgt aaaaattttc gctattacgc ttgtctcccc aaaccgggtt gtttttaata
     4861 cgatgccttc ggttttttct agcattacaa atgagttatt ttgataatat caaaaaaata
     4921 tagtgtcaat attaggaaat cgctgaaaaa tactataatt ttattgagtg taaatcattc
     4981 ccatgaagaa ttttagaaaa ggttttttat tttcattgat agttgtatct attgctacta
     5041 cctcttgcaa aaaagtttgg tactgcgatt gcaaatccgg aggcaaaact gtaacctcca
     5101 ctgcaattga taatgtaggt aaggtaggtg ctaaaagcgt atgctgggag tatgaatacc
     5161 aggctcatgt aaatggtgct aactacgaat gcaatattcg ttaagctgtc taccttctca
     5221 tcaattcttc tatctcttcc acttccaacg gaatatcgtg cattaaatcc atgatgccgt
     5281 tttctgtaat caatatatca ttttctatac gtatgcctat gttttcttcc ggaatataaa
     5341 tgccgggttc gcaagtaaat acgtttccaa cttccatcgg tgcatagcga ttgccaatat
     5401 catgcacatc tatgcccata aaatgggaag tgccgtgcat aaaatatttt ttgtacaacg
     5461 gattttcttc actttgtttg gctacatcat ctttattcag caaacctaag ccaatcagtt
     5521 ctccctccat tattttgcct acttctttat tataatccgc taaaatagca cccggtttca
     5581 acatggtttt agcctgtttc atcactcgca acacggcatt gtaaacatct ttttgacgtt
     5641 gtgtaaactt gccgtttacc ggtatagtac gggttaggtc agaagcgtaa ttggcatact
     5701 ccgaaccaaa atccatcaat atcaaatcac catcctgaca tgttttgtta ttctcgacat
     5761 agtgcaagac acatgcgttt ttcccggaag caataatcgg cgaataggca tgtccattgg
     5821 cacgttgacg aataaattcg tgaatgattt cggcttctat atcatattcc atcatgccgg
     5881 gacgaatggt tttcaatacc cgcaaaaaag catctcttgt tatttcacat gcctttctta
     5941 tcagttcaat ttctgttttt agttttacac tgcgcaatgc cgacaaaatg ggaccacttc
     6001 ttttgatttc atgtccggga taattaaact ttaattcatg agcaaagcgg atgtccttat
     6061 atggcacgtg atttaccgct ctgtcatttt cattaaagtt aagataaact ttttcacagt
     6121 aagccatcag agccggaagt gctgtcgaaa atgcactgtt ccagaatatc tctttgattc
     6181 cggaagcggc ttttgcctcc tccatcgtat atttatgccc ttcccaaaca gcaatgactt
     6241 cattggtttt gcgcaaaaac aatgcttctc tgtaaattgg atttggacaa tcgggatata
     6301 aaatcaaaat agtttcttcc tggtctattc cggtaagcca aaataaatcg gggttctgcc
     6361 tgaaagcaaa gttctgatcc gcactgcggg gcatctcatc attcgagaaa aaaattgcca
     6421 tcgaatttgg tgctaactgt gctgtaaaac gctttctgtt ttcaataaat aagtctgacg
     6481 atatgggtag atatttcata atgcaaaaaa ggatgaaaag caaagttagc attttatgtc
     6541 gaggtgaaaa aatataggct attgcaagaa aaatgccttc gttcaataat ttgttgattt
     6601 gaaaaatgct gaattccagt aattaatgca tttttttaaa gagctaaata aaatttgcta
     6661 cgtagtggct aagcctttat aaccatacaa actcaagtaa ctaagccacg tagtgggtca
     6721 cttttttgat aatacgaatc ccattttcac aagttcaata atctgagttc ggattaatag
     6781 aaataatatg ataaaatttt tgatttcgta ttttttatcc agcttaaata tgaggagtaa
     6841 ttaaatgatg gtagttcttg ataaaaaaat caacaatttt taaatttgaa atacttatac
     6901 cgaactcagg ttaatagtga aaaaataacc ctgaaaaatt aaataaaaat aaagagatag
     6961 aatctataat ttcgcaaatt ctaataaata caaagatctt cttgcttggg tctcctcctc
     7021 cgcttaacct tttattattc attcacttag ttgttttcgg gcaaattaag cgtacctttg
     7081 caattcctaa aaattaggca gtatgcaaaa tcagcaaatc agaaatgtag ccatcatagc
     7141 acacgttgac cacggtaaaa caacccttgt agataagata ctttgggaat gtaagacatt
     7201 tgacgaacac gaaaataaag gcgttttgat tttagacaat aacgaactcg agagagagcg
     7261 cggtataacc atcgtatcca aaaacgcagc ggtaaaatat aaagacttta aaattaacat
     7321 tatagacaca ccgggccacg ccgactttgg cggtgaggtg gagcgtgtat tgaaaatggc
     7381 agacggcgta ttattgttgg tggatgcatt tgaaggtccc atgccacaaa cacgttttgt
     7441 tttgcaaaaa gcgttacaac tcggtttaaa accgattgtc gttatcaata aagtagataa
     7501 agaaaactgc accccggaag aagtacacga aaaagtcttc gatttgatgt ttcacctcga
     7561 tgccacagaa gaccagttgg atttcccgac tttatacggt tctgctaaaa acggctggat
     7621 gaataccgac tggaaagtaa aaaatgacaa tatcgttccc ttgatggatg ccatagtagc
     7681 acatattcct gcaccaaaaa ttgctgaagg tgctacgcaa atgcaggtta cttcattaga
     7741 ttattcttca tttgtaggtc gaattgctat cggacgtgtt ttcaggggct ctatcaaagc
     7801 caatcaaaat gttgccttga ttaagcgcga cgggaaaatc acgaaatcca gggtaaaaga
     7861 gctgatgttg tttgaaggca tgagcaaagt aaaagtggat gaagtacatg caggcgaaat
     7921 ctgcgcggtt gtgggcatag aaggtttcga aatcggtgat acaattgccg atgcagaaaa
     7981 tccgcaagca atggcggcta tctccattga tgagccaacc atgagcatgt tgttcacgat
     8041 taatgattct ccattttttg gcaaagaagg caagtttgtt acttctcgtc atatcaaaga
     8101 ccgcttggat aaagaactcg aaaaaaatct ggcattgcgt gtagaacaag gtgatacggc
     8161 agatgccttt atggtatatg gtcgtggggt gttgcactta tcagttttga tagaaaccat
     8221 gcgccgtgag ggttacgaat tgcaggttgg tcagccacag gttttgttta aaactatcaa
     8281 cgaccaaaaa catgagcctt atgaagaatt gaccatagac ttgcccgaaa ctaaaagcgg
     8341 aacggcaaca gaaatggttg ccaaacgcaa aggcgatttg aaaaatatga tattgaaagg
     8401 tacccgcacc ttgctggaat ttgatattcc gtctcgtggt atcattggct tgcgtaccga
     8461 actgattact gcaacatccg gcgaggctat tatgtcgcac cggtttacaa gatatgatgt
     8521 gtttaagggc gaaattccgg gacgcattgc aggctctatg atttctaaag aaacaggcga
     8581 agccattgct tattctttgg ataaattgca agacaggggt aaatttttca tcgaaccggg
     8641 tgatgaaatt tacacgggca tggtagtggg tgaaaacagc cgtccggatg atttagtgat
     8701 taactgtaca gttaccaaaa agctgaccaa tgtgcgcgca tcgggcagtg atgataaagt
     8761 gaaattagcc ccacctataa aattcagttt agaagaagct ttagagtata ttcaatcgga
     8821 tgaatatgta gaagtaacgc cgaaatctat ccgtctaaga aaaatatatc tggacgagaa
     8881 tgaccgtaag cgctacggaa aataagccga aggattttta tattattttt ctttagtaaa
     8941 ctaaggttga atttatatgg ttgaatgcaa ctaaaagatt ctagatctta tctgagtaag
     9001 tatgaataaa tttctttttg cagtattatt ttcaatcctt ttcttaaatg cttgtgtgcc
     9061 cattggaaat aatgtcaata ttacaattaa tggaaatgta gaaaacaata gcgattactg
     9121 tggtggagct gcaccaccac aagagttatt agatcagttg gctatgtatc atcccagcgc
     9181 tatgcaaaag ttttatgtgc gtcagggaag tgttgctatg ccatttacac ccgtttatac
     9241 gttttttaca accgatagct tgggtaatta taccatctca ttaccggcag gacaatacac
     9301 cgttatcagc gaagaaaagt acctgacaga gtccaatgct gccattgatt ccagttgtac
     9361 ttatttgcat acgcccgatt tttctctaaa tatacaggcc tcgcaaactg cctatacgaa
     9421 cagctacacc attacctgca attatacctg cacaggtcaa atgcctcaat aataagtgat
     9481 gcatagttgg taggtttatg ccaatttgtc tttattttag ttggtattac catagtccat
     9541 atctttatat atttatacaa aaaaaatgac caagaaaaca gctgcaaaat attgggatga
     9601 caatgccacc acctggacga ccttagcccg gttgggttat gacacctata gagattttct
     9661 caatactcct gcttttatga aagcactgcc cgaaataacg gaaaaatacg gtttggatat
     9721 cggatgtggc gaagggcata atacctgctt attaacaaat gctggtgctc acatgcaggc
     9781 cattgatgtg tcgccagtat ttatcaacta tgcaagtcag tttgaacaag ctcagcgtct
     9841 gctattgagt atcaagtggc aaatgcctca cgtttgcctt ttcaggatca tacatttgat
     9901 tttgcaacgg catttatgtc gttgatggat atccccgatg tggaagaagc gctgaaagaa
     9961 gcctatcgtg tgctaaaacc aggagggttt atgcaatttt ctattacaca tccttgtttt
    10021 agcatgtcgc atcgcaaaaa cctgagagat aaaaccggaa taacttatgc gatagaaata
    10081 ggaggttact ttaatgctgc tgacgataaa attgaaagtt ggactttttc tgctttgcca
    10141 aaagaaaaaa gaacagattt cccaaaattt gaaataccgg tatttcatct tactttgagt
    10201 gattggttga atacgctcat tcaaacagga tttactatag agtatttaaa cgaacccatg
    10261 cctgatgacg aaaccgtgca tgctcatccc gaattgcaag atgcacaagt ggttgcctac
    10321 ttcttgcacg tacgttgcag gaagtaagaa aaagaataaa actataaaac cttcaaagct
    10381 tatccacatc ttttggcaac aattttgata gaacattatc agcatattta tttttgcatc
    10441 gtgagaaaga ttttagtttt gttagcaatc gggattggta catttttcaa tacttcagct
    10501 ttagatattg acggaaaatg gcaaggtact tttatcgagt atatccataa tacctatgat
    10561 gtacagatga tcatgaaagt actgccctac aatacctttc atgccaattt aaagattacc
    10621 aacggacaat atttcggcga gtataatgtg agtggaaata tctgctccaa ccgtagcctg
    10681 gagattacag ctattttctt aattcgcgag aatagtgcca acaattggat tgattgtttg
    10741 aatggcgttt gggatttaaa tgaagatgaa aatgaattga cattcaccga tacttggtta
    10801 aatcaaaatt ctaaaaaaga tgtctgtaaa gtaaaatttg ccaataaaga tatgtttcaa
    10861 tgcctgcgct ctgcctactt cagacgcagc accctgtatg attttaatcc cgatttcgat
    10921 tcgacttggg cacagtatac caataaaaat aaaaagcaaa ttatagacga aacacaactg
    10981 gcaaaaaaag aagataaagc cacgccgact cttgacacgc tcactcgtat caacgaccgt
    11041 gaaattaacg tgaaagaatc gatagaggtt tccggtgatt ctgtcattat tgaatattgg
    11101 gacagattta acgaagatgg cgactctatt actttatact taaacaacaa accgatagta
    11161 tataaaaagc tactaacgaa agtaaaacaa tcactgacag ttaaacttac ccaaaaaaca
    11221 aattacctgg ttttgcatgc agagaactta ggaaaagaac ctccgaatac tgccactatt
    11281 gcgataaaag acggcaaaaa aatacagact atatcgctca actccactct tagaaccagt
    11341 agtgctatta agctcgtcgt caaagataaa aagtagctgt ttggggtaaa acgagtattt
    11401 ttatcttgct cattagattg aaatagtcaa aaataaatta ctgctaaaat ttttgagaaa
    11461 ttatttagta aaggaacttt tatttacctc aatctgtttt caaaaaaaga aatactatgg
    11521 aagtaacgat ttggacttgg gcaggtttta tcgcatttgt cttactgatg ctggcacttg
    11581 acttaggtat tttcaaccga aaacataaag aaatcagtat tcggtctgca ttattatgga
    11641 gcttggtttg gatcgggtta gctatgattt tcaatctggg gatttatgca tttctcggca
    11701 aagcaaaggc agtagaattc ctgaccggtt atgttatcga aaaatcattg agcatcgata
    11761 atattttcgt atttatcatg gtattcggtt atttcaatat tccttcaaaa taccagcata
    11821 aagttttgtt ttggggaata attggagcat tgctcatgcg ggcggttttc atctttgccg
    11881 gggtagcctt aatcacccga tttcattgga taatttatat tttcggagct ttcctgattt
    11941 ttactggaat taaaatgctg acgcaggaag acaaagcact ggaaccggat aaaaatcctt
    12001 tagtgcgcct attcaaaaaa ttcatgccgg ttacggacaa actgtatgaa gaaaattttt
    12061 tcgtcaggat taaccataaa aattatgcta ctcccctgtt tgtcgtttta cttttaatag
    12121 agtttactga tttaattttc gccgtagatt ctattccggc tatactcgct attacttccg
    12181 attcatttat tgtttttact tccaatgtgt ttgctatact tgggctacgc tccctttatt
    12241 tcgctttagc aggcatcacc aaatatttca gatttcttaa atatggttta tccgtaatct
    12301 tgatgtttgt agggataaaa atgtgtattg ttcactggta taaattacca actttacttt
    12361 ccttgggtat tatcgtctct attttgttaa tctctgtgct tgcatctgtt attatcaaac
    12421 caaaagataa tacagtataa actgatagaa tgtattttta agctttataa tcgctttctt
    12481 taaccagttt atctgctttc atcgtagcat tacgataatc taaagccgag tttaaaaact
    12541 gctcataagc acccacataa gattgggcaa attcgcgttc aggttcatgt ttattgatgc
    12601 tcagcactag tttttcaaag ccccattcgc aagttatttc tccagttact acaaataccc
    12661 ggtcaaaatc ttgcaagatt ttgatttgtg tattgcattc tacttctttg ctcaataaaa
    12721 gtgattttgc tgaagtaaca aaacctgtat atgcatagta aatggcttca gcatacttgc
    12781 tgttatccaa tgcttgtaaa gcttctgttt gttttaattg tgcatctttc aaaatcgtac
    12841 tcaccatatc ataaaatacg ccggcacatt cgcccacgcc tacctccggt gtaaacaatt
    12901 catcatgccc ataatcataa aaatcatcat ctacaaattt tgaagtatca gcaatgggtt
    12961 ttaataatgt ataaaaataa ttctttcctt gtcggcgata ataattatta aaatattctg
    13021 tctcatccga atgattttca tagtcatcca agataatacg cagcgcttca ggcatctttt
    13081 tagttggcag ttttactact ttatctcctg ccattcccac accattatta tctactcccc
    13141 cacccaagca aatctgcata gcgggagcta ctttatcgcc tactcgaata gagctgccat
    13201 gcaaaccaat atcagcactc atgtgttgtc cgcacgagtt catgcagccg ctcattttta
    13261 tgtgaatagc cttatcaaaa atcaaatcat agtattcctt ttgaatgaca tcttctaaaa
    13321 tttctgccag taacatactg ttggtcactc caaggttgca agtgtcagtt ccggggcaag
    13381 tcgtaatatc ggctatggta ccaaagccgg gattcgcgaa tcccatgcta tccaaaacgg
    13441 aatacacttt aggcaatgct tctttacgca catacttgag caacatgcct tggttcatgg
    13501 taatgcgcat atcatccgat gcaaaatcgt tagcaagttg tgcaataaca cgggcttggt
    13561 cgggcttgat atcacccttg gttactttta aatacacccc ataaaatccg ggttgctttt
    13621 gttcaaaaac atttgtttcg agccagtctt catagcgttt tttatccttt atatctactg
    13681 tcggcacttg ttttatttcg ggagaacgcg ggctttcaaa accgtcagtt tccactggaa
    13741 aaacaagatg agcattcgct ttcatttctt cctgtatcaa tgtgagaaat tgatctaccc
    13801 ccaattcttt taccaaatat ttcatacggg ctttatgtcg tctggtgcgt tcgccgtaac
    13861 ggtcaaatac acgtaaacct gcctctacaa atggaattat tttatcgtct tccaaaaact
    13921 caaatgctgt ttgcgccaat atagcttgtg cgcctaatcc accgccaact actaccttaa
    13981 atcctcttat gccgttttca attttcggga taaaaccaaa atcgtgtatg taggtaaagg
    14041 cagtatcttt ttctgaagaa gaaaatgcaa tttttatttt tctacccata tcctgacaaa
    14101 tcgggttgcg caaaagatat ttaaagagtg cataaacata cggcgacacg tcaaaaggtt
    14161 catttttgtc gatgccggca cttgcagaag ccgtaatatt gcgcaccgtg ttcccgcatg
    14221 cctcgcgtcc tgttagtccg caatcttcga ggcctgccca aactaatggt gcgttatcta
    14281 cttttacata atgcaactgt atatcctgac gtgtggttaa atgcagattt gaagatccat
    14341 acaactctga caattcagca caccgaacaa gctgagccgg agttatgcgt cccatcggta
    14401 ttttgatgcg aaacatttgc acacccagtt gtcgttgccc ataaacacct ctggttagcc
    14461 tgaaatgttt gaatttatct tcgggtattt ctccgttgac gaaggcgttg attttttgtt
    14521 gcaactcaaa aatatctttc gctgcatccg ggcttacatt ttttgtaata gtaatactca
    14581 ttttttcttc ttaatctatt ataataaaac ctgtcttttt gagggacagg ttataatttt
    14641 tcacaacagc aacaacctac ccgtttttac ggaatgcaac aacaaaaatt gtaaaggctg
    14701 actaaattgt tcatcagaag taaaagtaat gaaaaatatg aagtataatt gttcttaatg
    14761 atttgataaa atttatctaa aaaatgctac ctttaatttt ttaaaataga aatatgaagt
    14821 atataatacc acttttcata tgcataatcg tgggtcagtc gtgtcagaag aatgaaaacg
    14881 agggttctcc aaaagaaccc ggtccgcctc agcttgcggc atatacagat tcgatgctgc
    14941 atgccgttgg cttacagtcc tattctgatg tgctgactgg aaaattagaa ggcaactttg
    15001 agatcttcga gtattgcact ccttatgatg tcaattgttc tgattttaca ccgcaaacgg
    15061 aattgaatgg gagtgtgtcg ttagatacca atcgaacatc taccatagac gcaggcgtgt
    15121 tgaaaattaa ccaactatct attacgccta acacaaataa gcgttacgaa attcattttg
    15181 gaacttcaca atacaatagc caacaagccc aacttaattt aatttatggt gcgacaaaca
    15241 ctatcaaact tgtaaaaaat ggagtggatg tgtttaataa aagcttgtat gtaccaaagc
    15301 atattaaata tacaggatat gattgtgcta catcaaaaat gaagaatgac cctatcaagg
    15361 caaatgacga gatatactgg aattcggatt attctaacaa gaatggcatt ctaatcagat
    15421 ttacgggtaa cgacagcagg ggaatagaat atacatttta caggatagtt tctgatcttg
    15481 gtaaatatgc cgtaacagaa agtgatttat cctatttccc aaaagagaaa aatggaatgg
    15541 gcatccaagt agaactaaac aggggaaact tcttcctcac ctatggtact gacggcagaa
    15601 gatacaatgt aaccattaat gcttcgtgta aatattattt caatttgttt tgaaaacaaa
    15661 aaaagatttt atctcattta ttccggataa aatctttttg attgtttatt atgataagac
    15721 ctaaaactgg taagcaaacc tcacaaaacc accattaaaa ttggttttaa agtcggtatt
    15781 caggttatat ccacccgtta agctgaaaga agcattttct tttacccgaa acaaaactcc
    15841 tgttcccaaa tttaaagcca tactgttttc tcccagttgc ttctgtgttt tttcgttagt
    15901 taaggctgct ttcccggttt tattcaatcc ggcttgaatg gtaaatgtcg gtgtaaccca
    15961 tttatttaaa gcatgtacac gcaaatcggt aagcaactgg aactgccatt ggttggtatt
    16021 gcgaatttga atacccacac cctctccaat aaatacatac ggattgagtt tggcaccaac
    16081 cgcttgataa atgctaaaga acggcttgcc gtaaatgtaa ttcatacctg tttctgtaat
    16141 tgctgatatt tttcgttcag cataatcgtt ttcattaaca tcttgggagt tgtttgcaac
    16201 agttaatgaa tcattggcgt atgcacttaa tccggtgcag atggccaggg taactaataa
    16261 ttttttcatc ttgtacattt tttagtttgt aataatttta taaagttcaa aactttcaga
    16321 caaaattgaa acttaatttt cacatggata ttaggtatgt gttacctttg ttgtgcattc
    16381 cggttataca atagctgtgt taatgttatt cagaactttc agtcattatt gaaactttag
    16441 gtaatataaa gatttgatag cacaattgtg ttatactgag aatatgctgt ttgtaaatca
    16501 acatattata aaggctaaac tactacgcat ccaattttat ctagttctgc ttaaagtaac
    16561 aagcttctga aaaaagaaat atgtctgaac ttagcacact taaattctcc tctcaataga
    16621 gacaagctgg ataaaacaat ttattttaat tttctgcctt tccagcggta tggaaaaacc
    16681 agacccaagg tgcctacaaa cggtatatac cagatatgta aaagctggct atatgcaaag
    16741 gattgcatta aatcgtggcg attgaagaag tttgttgctg atttcaaaaa agtgtaatct
    16801 atcaggcttt tgcataaaat ctgtataaac agaataatga atatcaaaaa cacattaaga
    16861 aattcaaata gcgcagagat agtaggattg gggaaaatgc ggtaataggc gattttgcca
    16921 aatacaaaaa agaaattaag ccaaatactc cacacaaaca gatagaccaa taataagata
    16981 aatgagatgc gtttatcttg ataatgcccg gctttgcttg tccagcggaa ccgttgctgc
    17041 aaaaaatcct gaacattctc tgcgggatgt gtcaatacaa tggcagcctt attttttaga
    17101 aagcggattt tatccggaaa tttctgtgct attttataaa ccaaaagcat atcgtcacca
    17161 ctggcatttt tatcaatatt tttatagccg cctacctcat caaaagcttt tttttcgaaa
    17221 gccaaatttg caccatttgc catattaaaa attttcatat tcagtgatgc gcccgtaata
    17281 gcttgcatgc cacaaaaatc caaggattgg aaatgctgaa atttggaatg gtcattataa
    17341 aatccgactg gactggcaat taatgcacat ttttcttttt cataaaatga aacaatgctg
    17401 tgcagccagt gcttttgcag tcggcaatcg gcatcggttg ttattatcag cgtaccgttt
    17461 gcttgtgcta tgccatattc gatggctctt tttttataag cttccgtaat ggtattctta
    17521 tccagactat cttttaattg taaaactttt atcgtcgtat ttgctttgct atatgaagca
    17581 gcgatggaga atgtattgtc ttccgaaaaa tcatctatta caattatctc cacgagggaa
    17641 gcaggataaa tctggtcgag taaatccttt aaaatattgc ctatgtattt ttcttcattc
    17701 cgggcaggta cgataacaga aacacgtgtt gtacattctt ctctcaaatc gtgggtttct
    17761 ttgatttctt cccattcatt tttatacgtc cacataatgc cgacataagc gacagtaagc
    17821 ataagcgata tgagtaacca gatgagcatt attttttcag tttttcttcc agcatttctt
    17881 tcagttttat cattctgttt tgcagggttt cctcacgttt tgccgaagtt atccattgta
    17941 catattcttt ctgatgagtg tatgataatt tatcaaaaac ggattttaat agattttctc
    18001 tattaaaaaa atcaactaaa acggtgggta gttcaacgat tctttcttca aggtcttgtt
    18061 caattttcac gttgacgata tcgcctgctt ttttgttgag ttgagcgcgg atagccttgt
    18121 ttatcatcaa gaaatagcca ccgtcatatt ttgcaattaa accgcgataa agtacatcgt
    18181 tataccagat tttagccttg aatcttttct tgccaaatgt tttttctacg tctagctcta
    18241 atggaatata aaagacatcc atcttttcat ctttcactat tttggctttg aattgtacag
    18301 tcatatttta tctttgttac atattattct atattatgaa taagctgata agtttgaaga
    18361 atctcatcca atatagtgaa taaattctca cgttggttgg tggttaccag cttggctctg
    18421 aatagcttta tgttgggtaa tcctttcagg taattactgt aatgctggcg catttctaaa
    18481 atgcccaaaa cttctccttt ccattctata gatttttgca aatgagtttt gattacttgt
    18541 attctttctt caatagaagg ctcgggtata agtttgtttt caaacaggaa tcctttcatt
    18601 tgttgaaata tccagggcgc accaatggca gctctgccta tcataatgcc atccacaccg
    18661 tagcggtttt tatattctat cgctttttgc ggagtatcaa tatcaccatt cccgaatatg
    18721 ggaatatgca tgcgaggatt attttttact tcaccaatta gcgtccagtc tgcttcgcct
    18781 ttatacattt gctttcgtgt tctgccatgt atactcaatg cctgaatgcc gatatcttgt
    18841 aaacgttctg ctacttctac tatattttta gagttttcat cccagcctaa gcgtgtcttt
    18901 actgtaacag gaagttttgt cgcttttact acttgtgctg tcaaacgcac cattttgggt
    18961 atgtcttgca aaataccggc accggccatt ttacagacta ctttactgac agggcaacca
    19021 tagttgatat caattaaatc cggttttgcc tgttctataa tgcgtgcact ttctaccatg
    19081 gcatcttctt cgccaccgaa catctgtatg ccaatcggtt tttcaaaatc gaagatgtct
    19141 aatttttgaa gacttttttt agcatggcgt atcagccctt ccgaagaaat aaactcggtg
    19201 tacattaaat cggcaccata ttgcttgcat acataacgaa atggaggatc actcacatct
    19261 tccatgggtg cgagcagaaa aggaaaatct ccaagttgta tgttgcctat agttacgatc
    19321 gtagtattat tttagatgaa agaaacaagt gaattttaaa gctgcaattc aaaaaaactc
    19381 cgttactttt acacaaagat acataatgag cgaaactatt ttagatgact tacagttggg
    19441 gcaaaacaaa ctcttgtatg gcattaacca aaaagacgga tttactcaat ttttgatatt
    19501 gatgggcatt tttgttgctt gttttattgc agggcaatta ctcagcgctg gattattata
    19561 tctctcgtat gggttggatg ttgcagatat gtcaacgcta atgaagaaac ataaagattt
    19621 gaatgcattc agatatgcgc agatgctggc taccgttttt agttttttgg cacccgccat
    19681 cattttttcc aaacaaaaac aaaagaactg gctgcagtat aacagagcag acaagtcatt
    19741 taatcttgtt ttaattgcct taattccatt attgattatc tcattttatc cactcattaa
    19801 cgtgtctttt tttctgaata agtcattggg catcagcaac tggtttcagg gtatgcaagg
    19861 agattacaaa gaaattgttt tggcactcac tgccggtact tctgtttcta ctcttatttt
    19921 aaatttaatt actgtggccg tgttaccagc catctgtgaa gaatggtttt tcagaggaac
    19981 catgcagcgc tttttctcag aaaaaatgaa tattcatctt gctatctttc tttcttctat
    20041 tgcgtttagc ttgatacact tagaattttc aggatttttg ccgcgtatat ttttgggcat
    20101 gttgttaggt tatatatatt attacagtgg cagtttatgg gccagtatat tcgcacattt
    20161 aatcaataat ggggcacaag tcgttgccat ttatttgagt aacaaaggac tttatacagt
    20221 caatatggac aatccggatt ttcctactta tcgggaatta ttaatcttta cacctttgtt
    20281 tattgcttta ttttttggca tgatttattt atctaaacag aaaagaagta cctttgcctg
    20341 attttttatg aatttaatct gattttttat gaatgatata ggtaccagaa ttgcaacagg
    20401 cattattttt ttaattgtaa tgattggttc tgttatttgg aatcagtatt cgttcggtat
    20461 cttattcgga ttaattgtgg ttttgtgtat tagtgaatat caaacaatca ttgacggctt
    20521 attagttgct actccgagat ggaaaacact gtataagtat atcaacttaa ttttcggagt
    20581 aatcacattc gttatcgttt atttagtggc tgcaaaaaaa ctaccgctga tgtatatgtc
    20641 ttttgtgtgt ggcttcccgc ttacctggct ggtaatagag atgtacacca aaagcgaacg
    20701 accgttttat aatgttgttt taaattctat gccgatttat tatctggcta tacctttgtc
    20761 atcttccagt ctgcttgcct tccacgatgg tggatacaac tggcattatc ttttagccat
    20821 tatgattttt gcctggtcta atgattcatt tgcttattta gccggaagat ttttcggcaa
    20881 gcacaaatta tttgagcgca tttcgcctaa gaaaacatgg gaaggatttt ttggtggaat
    20941 ggcaggttct gttttgtttg cctatctcgt gtttctttta tttccaaaat ggacgcacca
    21001 accggttagt cttacaacct atattgcctt agccataatt actactatta cttccactta
    21061 tggagacttg gcagaaagca tgataaaacg cagtttgcaa attaaagatt caggctcagc
    21121 gttgccggga catggcggat ttttagatcg ttttgacggg ttgctgtttt cacttccggc
    21181 atgtaccttt tatcttacac tatttttacc gtcgccggta tgattttccg gtaaaggcat
    21241 atcgcacaga aagtgcaatt tcgtaataag atacgctggg ttttactttt atagagttat
    21301 tgcgaatgta tttatcaaaa tagatagtag gcttataatc gaaagtaaag tttatcggaa
    21361 acttgcgcaa taaaacatcc acaccaagta taggatcgat accaaatgca aaaatattcg
    21421 tcgtgctttt atttccatag cccaatgaca gaccgccacc taataaaaaa ctccaacgtt
    21481 cgtcccggct cagcatccag tgtttttcat ataatgcaga aatattgata ccgggttttg
    21541 tgaatactac tccatgatat ccgtatctaa gcaagccctc aatggcatga cccgaacttt
    21601 tgccatagcc attgctgttg aaaaaatatt ttccgcttaa accccagcca tagccaaaac
    21661 gcaagccgat ggcgccttta tactcttgtg caatgcccat atttatggac atacaaatta
    21721 gcaggaatga tactattttt ttgtgcatca ataaatataa gataattatg tcactcaata
    21781 ttcttcaata gaaaattttg attttaatca gtctgaatga aaaattactt gcttttcttg
    21841 atattgtaat tattagattt cgtcgatttc tgaattttag acttctcgta aacattcttt
    21901 ttatcaatat ctttgatgtc ttctttatat ttatcgacat tagattttac cttattaatt
    21961 tttccggttt tatccttgct ttgattggcg tttacaaaac cggaaacttt ctccattttt
    22021 gaagcagcta ttgtataatt tccttgatcg atatcgcgca tgatatcatc catcatttct
    22081 gtagcttcaa acataaccac catttcactt accgtactat ctaaattttg ttttatcaaa
    22141 atggtatctc tggtaagatg cagggtgttt accacaacta tattttccgt gttatatgac
    22201 tgcgcatcga tataattcaa tgtagaagtg aaaactaaat cttgcacaaa cggttttttg
    22261 attttgaatt tgaccagtat atttttttct tctttagaat aaacatcatt aagagaaatg
    22321 agtagtcgca tgttctccat tttatgattg tagccataag tcttctccgg aattaaaaat
    22381 tcagatgcgt aactgactga gatttttgct tgttgcgcta caacagacaa taggccgtcc
    22441 agctctttgg caaaaattcc cggaatttta tccggactgt caatatagta ataattaccg
    22501 ccaccatttt ctgccatagc cgtcatcaag tcttcattaa acccctcgcc caatccgaat
    22561 gtagaaacac taatacctgt ttctttaaat ttttgttgta ctaaatcttt gagctttgcc
    22621 gtatcggtaa ttcccacatt ggccaatccg tctgacagca ataatacacg attgacaaat
    22681 ccttcttttt tagtagacaa cacttgctta tatccttcca aaagcccccc actcagattt
    22741 gtagaacccc ggtctaaaat cttattaatt ttttcagtaa gtacttgttt gttttttaca
    22801 aaatcagatg cacttactac ttctacatca gaatcataat ccactataga aacgatatca
    22861 tcagcattta attgctctac gacaaatgca gcagcctttt ttgcgtattc taatttttca
    22921 ccatgcatag agccacttct gtccaagact atggagagat ttaaaggagc tcgtttttta
    22981 tctgacttta attcattgcc ggttactttc aggtacaaat atacatctgc actgtcggaa
    23041 cttattgcat acttgttttc cagtgcagct gaaaactgaa cagatttggt ttgtgcactt
    23101 gctgtaaaaa acagtaaaca tgcaataatg aaactactga ttttattcat agtaatagtt
    23161 ttagaagttt atacttttga tatgaaaata gtcgtaaact accggatcaa ttgcaaattt
    23221 ttcaccggtt tttaaattcg aaataattac aaaatcatgt tctttaaaga aagggtgttt
    23281 aattaattta ttcagattga ttttgcacat cttaatctct ccaattgcag gttttgcctg
    23341 ccatattgaa ctatcatatt tataaaataa atatgtgaaa tattgtgcat aacctataca
    23401 gtttgcctta tgcgtatttc ttaattcatg tggatttgat gaacaagttg taaaggtaaa
    23461 ctgtaaatca ccagctgtct gttttagaca atcttctaca acaatgtcaa ctccaatatt
    23521 attactattt acttttttat ctttatctaa aattgaagca ggtacagtca ttaatttcct
    23581 gtatgattta aattcataat gtacaaataa tttgaacagc atttctctga atactataat
    23641 cagaaacaaa atgagaataa aatatttgcc aaaatttttc atagaacaga taacgcaatt
    23701 taaaggaaat tgttacagtc acatggggtt ttattcgtta ataaatgcta aaaaataaat
    23761 tcaacagccc tctttctctc taatattata ccaatcgtac cttcttggat tagaaattac
    23821 aatcacaatt gattgcatcg tcaattttac ttaattttac agtatgagag atattttagt
    23881 tattggttcg ggtatagccg gtctgagctt cgcacttaaa actgcacaac gattccctga
    23941 taaaaagatt cttatactta ccaaagcgaa tgaagatgaa tgcaacacga aatatgcaca
    24001 aggcggcata gctgttgtaa cggatttctt gaaagataat tttgaaaaac acatagcaga
    24061 taccctaatt gccggtgacg gactatgcga caaagaggtg gtagagttag tggtacgcga
    24121 aggcagagag cgggtaaatg aacttatagc atggggaaca aattttgata aagatgaaga
    24181 tggagattat aaattgggca gagaaggcgg tcactccgaa tttcgggtgc tgcattacaa
    24241 agatgtaacc ggaaaagaaa ttgaaagggc attattggaa aagatacatc agcaaaaaaa
    24301 tgtagaaatc tcacagcatc attttgtcat agacttactt acccaacatc atttgggaag
    24361 tatcattact cgtgtaacgc ctaatataag atgttatggt gtatatgtac ttaaccgtct
    24421 caccaaccaa attgagaaaa tagaaagtaa aattacgcta ttggctacag gtggttttgg
    24481 acaggtttac agagccacta ctaatcctat tatagctacc ggcgatggcg tagcaatggt
    24541 ttaccgagcc aaaggcagag taaataatat ggaatttgta caatttcatc ctactgcatt
    24601 atttgaaccc aatgtaagtc ccaatttctt aattactgaa gccgtgcgcg gtgacggagg
    24661 gattttgaag cgaaagaacg gagaagaatt tatgcaatat tatgacgacc gtttatcgct
    24721 tgctccacgc gacattgttg cccgcgccat agataatgaa atgaaaaaaa gtggtgaaga
    24781 ctgtatgtat ctcgactgcc gccacatgga caaagagaat tttttacacc attttccaaa
    24841 tatttatgaa aaatgcaaat ctattggtat tgatgtattc aaagacatga tacctgttgt
    24901 acctgccgca cactatgctt gtggcggtat agatgtagat gaatatggca gaacatctat
    24961 aaataatcta tatgcttgtg gagaatgcag tcataccgga ttgcatggag ccaaccgctt
    25021 ggccagtaat tctctgctgg aagctgtagt atatgctcat cgcatctttg aggatgttgc
    25081 tgagaagatt gatgcaatat cctatccgac agaagcaatt cctgaatgga acgctgccgg
    25141 aacaagtgac ccgaaagaga tggtcattat aacacagagc attcgagaac ttaaagatat
    25201 tatgagcaat tatgtgggta tcgtgcgtaa taacgtgcgt ttaaaacgcg ctcaggaacg
    25261 tctctattta ttgtttaaag aaacggaaga gctgtatgaa accactacga tatctccgca
    25321 actactagag cttcgtaaca tgattactat aggttattta attaccagaa gtgcgttact
    25381 cagaaaagaa agcagaggtt tgcacttcac cacggactac cctactcatt acccgcacgt
    25441 agaagaaact ttattgtaac agcagatgag ttccgagaaa ttcttaacca cttaagggtt
    25501 ttatttttta aaccattaag gcatgaagga aatgaagaca gattcttaat gcatcttaac
    25561 ttcttaatgg ttttattttt taaaccatga agacatgaag aatactaagc caagttctta
    25621 atggtactta acttcttaat ggttttattt tttgaacctt gaggcatgaa gaatactaag
    25681 ctaaattctt aataccactt aacattttaa tggttttatt ttttgaacca ttaaggcatg
    25741 aaggaaatga agacaatttc ttaatgcatc ttaacttctt aatggtttta ttttttgaac
    25801 cattaaggca tgaaggaaat gaagacagat tcttaatgca tcttaacttc ttaatggttt
    25861 tattatttaa accattaagg tatcaaggga atgaagacaa tttcttaatg catcttaact
    25921 tcttaatggt tttattattt gaaccattaa ggcatgaagg taattaagcc aagttcttaa
    25981 tggcacttaa cttcttaatg gttaaccttt cgactattaa acacaacaga tatagcattt
    26041 tcaaattgcc atttttactt atctttgcga catgagtcag gtagatttgc ccaaaataat
    26101 agcgcattgt aaagaatatg gatttatttt tccgtcgagt gaaatttatg ataaccttgg
    26161 tgccgtatat gactacggtc cttttggggc tgaactgaaa aatcacatca aagaatactg
    26221 gtggaagagc atggtgcaaa tgcatgagaa tattgtggga attgacgccg ccatttttat
    26281 gcatcctact acatggaaag ccagcgggca tgtcgattct ttttctgacc cgttaattga
    26341 taataaagat tcaaaaaaac gctacagggc agatgtttta ttagaagaaa aaatagaaaa
    26401 actgcgggag aaaggcgaaa aagaattaac caaaaagaat ttatcggatg catctgttga
    26461 agaaaaagca aaagcctcac gcgattatgc caaagctata actctggaag cagaatatat
    26521 caaggcggcg gaagcagcag atttcgcccg ctacaaggca ttaatcgaag aatacgaaat
    26581 tgtatgtgaa gtttcaggtt ctaaaaactg gacagatgtg cgtcagttta atttgatgtt
    26641 cgataccaaa ttaggctctg ttgctgatga tgcaaacacg atttatcttc gaccggaaac
    26701 ggcacaaggt atttttgtca acttccttaa cgtacaaaaa tcagcacgta tgaaaattcc
    26761 gtttggtatt gcccagatag gaaaagcatt tagaaatgaa atagtagcac gccagtttat
    26821 cttccgtatg cgcgaatttg agcaaatgga aatgcagttt ttcatcaaac ccggcacaca
    26881 aaaagaatgg tttgactact ggaaaaaagc ccgaatgaaa tggcatttgg cactcggtac
    26941 ggcacaagaa aacttgcgct accatgtgca tgaaaaatta gcacactatg ctgatgcagc
    27001 agaagatatc cagttcaaat tccctattgg atttaaagaa ttagaaggca tacactctcg
    27061 tacagattat gatttgggca gacatcagga atactcaggt aagaaaatgc aatatttcga
    27121 ctctgaactc aatcaaaatt atataccata tgtcatcgaa acgtctatcg gattagaccg
    27181 catgtttttg cgagtactga gtgatggttt gaaagaagaa aaactggaaa atggcgaaac
    27241 acgtgttgta ctgaatattc caccggcatt atctcctata aaagtggcag tgttgccttt
    27301 ggttaaaaaa gacggattgc ctgaaaaagc ccgtgaaatc atggatacac ttcgttttgt
    27361 aggacgcact ttttacgaag aaaaagacag cataggcaaa cgctaccgcc gtatggatgc
    27421 cattggtacg ccttactgta taacagttga ccatcagacc ttggaagaca ataccgttac
    27481 cattcgcgaa cgggatacga tgacgcagga acgagttgcc attgataaat tggaaagcat
    27541 cgtgcgtgaa aaaacagaca tgaacgtgtt gttgcgcaag gcaatggcat agtttatgct
    27601 gattagttat aaaaatgaag aaaataacct tcatcatat
//
LOCUS       DAOQII010000003        26248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_823_length_26248_cov_4.755545, whole genome shotgun sequence.
ACCESSION   DAOQII010000003 DAOQII010000000
VERSION     DAOQII010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26248
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_26248_cov_4.755545"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1647)
                     /locus_tag="PLF48_00320"
     CDS             complement(34..1647)
                     /locus_tag="PLF48_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47791.1"
                     /translation="MKRILSLIFFSVCLCLASAQQLHYYLNYEMQNRLERYLSGSSGV
                     HTAVKPYNMSEILKAIPDSALQELGFITDSTDKFRLGLTKGDFLSVSAKKKKFYFALN
                     PIIDAQVGYDVLDKKLKYAVGYGAKFEANLGRKVSLSFTYQGDFEKMDNQVTAYAQKY
                     GVLAGYRKAHFGKNGLRSDMMSGYIRFAPNKYVNLELGNDKVFWGDGYRSLFLSDNAS
                     NFPYLKMSTQFWRIKYTYLLAGMRSGDTYGFNTENNYKNFKTKFGSFHYLSVDVAKWM
                     QFGFFESVVWYHADSLRTRGMEFSYLIPLAFLRPIEYALGSPDNVSFGISMKFNPNDK
                     QHIYTQILFDDMDVRAARAGKGFYRTKIAAQIGYKAFDIFKLKHLDFQTELNIVRPYV
                     YAHKSPQQNYANFNQSLAHPAGANFMESLVFVNYWYKHWTANLKFQYLVQGLDEPFAH
                     NGSNIFISDYMLGSNLNQAYGKTFLQGVKTRMYSVEARGGYLINPKINLSTEAFLNFR
                     YRENKYFKEQNLLFGISLRTNIFNRYTDF"
     gene            1745..2197
                     /locus_tag="PLF48_00325"
     CDS             1745..2197
                     /locus_tag="PLF48_00325"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HNF47792.1"
                     /translation="MAKGFSLKNDIKKINWYPPYLGAGISVTAVNDNFTRIEVQLKST
                     WYNKNLFGTHFGGSLYSMCDPFFVFIITQYLGKGYIVWDKAASIQFKKPGTGTVKAVF
                     EIEEETLKKVKSEIDELGKKDYPFSATIKNSADEVVAEVEKLIYVRKK"
     gene            2321..3106
                     /locus_tag="PLF48_00330"
     CDS             2321..3106
                     /locus_tag="PLF48_00330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF47793.1"
                     /translation="MLDQTSLIKCENCGIVYNQHTSSEKETTYYKENYTLRKNNISLQ
                     SELRRFSRWPEQLKLLADIKKTKPEAANIIDVGCDKGYFIDEARRFGYSVYGVELSKD
                     ARKYTDQLGLTVKDSVEEIGEKADIITMWHSLEHFSEPLSFLKSLHPHLNSQAHIFIR
                     VPDFGCFFSKVLKQKWRWFQPENHYFHYTEKSLKHLFEEAGFQVVQCRSQNPSSCLTD
                     KAFSLANKTLRKYYNEPASLKYVLGTYYERITCKELYLIAKTS"
     gene            complement(3168..3689)
                     /locus_tag="PLF48_00335"
     CDS             complement(3168..3689)
                     /locus_tag="PLF48_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47794.1"
                     /translation="MITVKNALEKYYGFNVVLLPETVLPPNAFTPVKSPRYRADSLLL
                     YLQKIKPDSVNYIMGITEADISTTKKDASGKIKNPATKYADWGIMGLAFRPGNAAVIS
                     TFRIKSSDKALFAQRLEKIALHELGHNLGLPHCPSKSCFMNDANESISTIDNEKTELC
                     SSCKQHLRGQFAY"
     gene            complement(3845..4426)
                     /locus_tag="PLF48_00340"
     CDS             complement(3845..4426)
                     /locus_tag="PLF48_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNF47795.1"
                     /translation="MKLPLSYFHNDDVVFLAKDLLGKILFTNIDNQITAGIITETEAY
                     CQSEKGCHAYNGKRTRRTEIMFQQGGYAYIYLCYGMHYLLNIVTGKKDVAQAVLIRAI
                     YPTIGISVMLERRHQTKLNKNICKGPAKVAQALGLTKSQNETLLQEQTIWLQENDYPL
                     SDGLITTSKRIGIDYAGADADLLWRFNLEYHTL"
     gene            4559..5257
                     /locus_tag="PLF48_00345"
     CDS             4559..5257
                     /locus_tag="PLF48_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNF47796.1"
                     /translation="MAYGKLFLIPNTLHEGNYVSLIHLVPIIASTDIYIVEEIKSARR
                     LLKGVLKTKDINSCTFKILDKHKDYQVHFSILDEIKQDKNIGIISEAGLPCIADPGFQ
                     VVQLAHEMGVEVVPVSGPNSMMMALMASGFNGQSFTFNGYLPIEQTERKNKIIQLESF
                     AKKNHTQLFMDTPFRNNKTLEELLKYCKPDTLLCIACNLMAKDEFIQTQTIADWKKIK
                     PDLNKKPCIFILGK"
     gene            5265..5843
                     /locus_tag="PLF48_00350"
     CDS             5265..5843
                     /locus_tag="PLF48_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNF47797.1"
                     /translation="MILIDDILISDDVVEKEFVCNLKKCKGICCVEGDSGAPVEKAET
                     KILKKIFPKIKKYLTAEGIAAIEKQGTYVDEPDDEYTSFATPLIDGGACAYINYADDG
                     TIVCGIENAWRDGVVDFQKPISCHLYPVRIKPMETVTAVNYDVWDICSEACLLGKKLK
                     VPVYVFLKDALIRKFGEDFYAALEYAAKDNEV"
     gene            complement(5894..6616)
                     /locus_tag="PLF48_00355"
     CDS             complement(5894..6616)
                     /locus_tag="PLF48_00355"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNF47798.1"
                     /translation="MNYKLLIFIFLLLLIPASAYIYKNECKESEKIVSVKLNPQSDSI
                     RFYYKDKSGNLYRSINNLKFDVEQKDEGLRFAMNGGMYMQDNSPQGLFIQDHRIVKSL
                     NTDIGYGNFYLKPNGIFYLMNDNKPALCKTEDFRFSTSIRYATQSGPMLVIDGKINPV
                     FNIQSTSLYIRNGVGLLPDNKVIFAISKQKINFYEFASFFKQAGCTQALYLDGYVSRM
                     YLPEKNWIQTDGDFGVIIGCVH"
     gene            complement(6654..7640)
                     /locus_tag="PLF48_00360"
     CDS             complement(6654..7640)
                     /locus_tag="PLF48_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47799.1"
                     /translation="MKAEIKKIYDAHVQFELEQFGKKHIKSNIKEDVHAVFKWLEKVT
                     LNDITSAEDIKSFLKRNVKHYELSDEQRDYAKDLAKHIHEHAKKSKHTVSNYITEKRF
                     FEIADKLIAQKEWREELIERIAHNPVYGEIIADTLYDGIKQFMQQSGPSNESVGGSLF
                     NIGKGLLGAALSGVQDNIDSTVKKFIAANLSKTLQKSEQNLKDRFSEPKLRLMSKTIW
                     NKIHDVKISDYAKKIKTKDINEGLDIAEQVAGDILKNATTEEISDFVLDHFFDYVGDK
                     NIVQLLDENGVRMEKVIKETEEFAIPVIEKARKDGFLQERIEARLSKFYKTL"
     gene            complement(7703..8299)
                     /locus_tag="PLF48_00365"
     CDS             complement(7703..8299)
                     /locus_tag="PLF48_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF47800.1"
                     /translation="MQKPTKNIEVYQEQLKKIIQQIADIKPENLDEFVKAFELEFIPK
                     KTFILREGEYSEETRFVIKGLVRIYYVREEKEITNWFISENMFFAAVYAILTGERNYN
                     YYETLEDTICLKLNYRKLEEFYAKNHCMEHFGRKLIENYYSLFMKKSYEVLFLSAEER
                     YHTFVKERGDLLNRIPLRYISSYLGITQETLSRLRAKH"
     gene            complement(8316..8912)
                     /locus_tag="PLF48_00370"
     CDS             complement(8316..8912)
                     /locus_tag="PLF48_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF47801.1"
                     /translation="MKSYKPNENQLNVLRHVANAVVKMDDEVIEEVLALCMVVEIEKN
                     TDLILPGDVFRDVYFILNGLVRVHYTKEDKDITFSFKSEHNFFTNSNYLYGHIQNFYY
                     YTTLEKTVCLVFNYDKLEILYKKHHALETLGRKVVEQNYINALYNNNNILYLSAEERY
                     IRFLKTQSALLQRVQLKYIASYLGLTPETLSRLRAKYS"
     gene            complement(8927..10336)
                     /locus_tag="PLF48_00375"
     CDS             complement(8927..10336)
                     /locus_tag="PLF48_00375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNF47802.1"
                     /translation="MAEYKFLLPKMGESVIEATVLQWHKQPGDKINEHDVLVEVATDK
                     VDSEVPSPVSGVLKQILHDKDAVVPVGDVLAIIEIEGSEIQVTPSNKVADKEVVVETK
                     NEIKPPEFKDIELKVENAIPEIKAVENTVEQAKVSVQYTAPVISSSAKYYSPLVMSIA
                     QAEQISMTELDALQGSGMDGRVTKEDILEYADKKKKGLIVTPVIPTVQKEKREATSTT
                     ETGQKKDSKQTNAEQPNTEIIEMDRMRKLIAQHMVNSKKTSAHVSSFVECDVTNIVRW
                     RDKNKNAFQKREGFSLSFMPVFVEAIVKAIKDYPMINVSVDGDKIIKKRDINIGVATA
                     LPSGNLIVPVVKQADQKSLLGIAKAMNALVNKARTNSLQSDDIADGTYTISNIGTFGN
                     IIGTPIINQPQVAIMAVGAITKKPVVLEVDGQDVIAIRHMMYLSHTYDHRVVDGMLGG
                     LFVKKVADYLEKFDLNQEI"
     gene            complement(10380..11645)
                     /locus_tag="PLF48_00380"
     CDS             complement(10380..11645)
                     /locus_tag="PLF48_00380"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNF47803.1"
                     /translation="MRGIVICIGDELLIGQTLNTNAWFISQKMNETGIDIIRHISIAD
                     EKNSITKTLDEALALADVILITGGLGPTSDDITKKVLCDYFGGTLVFNEKAYENIERI
                     FSLRQREINEATRQVAFLPDVCIPIPNKNGTAAGMLFRNENKVVVSMPGVPYEMQAMV
                     TDYFIPYLQQNFSLPLILHKTILTAGVGETQLAERLTVFEKNKHPQVKLAYLPSVGKV
                     KLRLTIKSEAENHAIQNTTLYELMEQSRNEVLAAIGEYVYGFDEETLEANIGKLLKDK
                     KLTLSTAESCTGGTIAQMITSVAGSSAYFKGSVVAYSNDVKEQVLHVQSATIQRFGAV
                     SEETVAEMLDGALSVLHTDLAIAVSGVAGPGGGSAEKPVGTVMIGIANKNQQYIRRLS
                     FTNHRGRNIELSSVVALVMLRKFVLKYYS"
     gene            11717..12439
                     /locus_tag="PLF48_00385"
     CDS             11717..12439
                     /locus_tag="PLF48_00385"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNF47804.1"
                     /translation="MKKVFVILLSSFLLFGCTSQQIQDTVGSILGYSGTPTDAEAGNG
                     LKEALSIGIVNGMGLLSKTDGFLGNDLVKIPWPDQAKPVMDAMLKLGMQKQVDNVTTS
                     LNRAAEQASGVAIDVFKQAIMQMTITDAMNILLGGNGSATAYLKKTTTPILTEKFRPI
                     VDNSLGKVNATKYWSDAITVYNKIPFVKPVNTDLTGFVTEKALQGVFVMVEKEENKIR
                     ENPLARTSELLKKVFGFADTKK"
     gene            12509..13645
                     /locus_tag="PLF48_00390"
     CDS             12509..13645
                     /locus_tag="PLF48_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNF47805.1"
                     /translation="MSRKPQQGPLQGIRVLDLSRLLPGPLATQLMADMGAEVIKIEDP
                     NAQDMTRFFPPMIKNQSLFYLSINRSKLSVTLPLKMEEGVQQFMELVKTADVVFDSFR
                     PGVLDKMQIGYEDAKKVNPKIIYVAVTGYGQTGTMKNKAGHDVNYLGYSGILGMTGLK
                     NQPIMPGVQIADTAGGSYAAVTACLAALWARERTGKGQLVDVAMTDCVMPMASILYTK
                     KLNSDDDSLRRGFDYLSGGLAHYNIYQCQDGKWIALGSLEPKFFQGFCMMIGKPDWVM
                     RMLPEEKTVESLREDLAALFLTKTQAEWLQMAAGQDICLTPIMDLDEVYNSDYAKERE
                     LFFDMEHPAYGTVKTVNQPFKFSETPAKPSWGAPLLGEDNEMLK"
     gene            13869..15836
                     /locus_tag="PLF48_00395"
     CDS             13869..15836
                     /locus_tag="PLF48_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47806.1"
                     /translation="MTPEEFNRITKQCQPHKVFKLDWKTYYSEDFGNTFLPDIEPIGG
                     KAFLMYDGADFFRNLIPTERKLLKLTRGTELISLTFYTYPKGVLAQKRKDRVSVEDFE
                     ICAFIEVDENDKLEIFFKFGRNFKDFLYKYFTENKINKNPEAVEKTIYKSNTKELMGY
                     FNNSIVHYSLMAYVGKTIGVDLVAVNKKVAYELKINKISISSSTLSGNLNKHIKSDKE
                     EYINIDFTIYNYPSTEYRIIIYKNNEKIDELVFLPEIVIIPNKEKIDTQTENTLYKAG
                     KHRIVYECSNTDILIGKNENDKITLRIQATNENKEIAYDQKELLLKIAMQKAATNNYI
                     LLVDPNKKEGEKDCYIKIYANEILKKQKIIKNKIPKAEYNKLSVEEKLIIDLPIILVK
                     LDWLYSAYCQLYWLEGSGEDIYLPISFVESYARVQDALKQMKNDFSEIEYASYMDETN
                     YNPNLSIDDSSNRFLYNISLNQVKLSYNKATAILNKITDFGEYDFGNFDMKEANPKLN
                     FVRSYTIGSHIGKVATGDKDDLGGALGLFALRIYIKGKVTKQEFNSNQVRLFYLNISN
                     VGYRYFDDFSFDGFQPLGAWRYDLDNPVPPEKLTYKRDKWYSLWNETYRTLQNRKNTN
                     LSPKSFYIITGIKQLKNHNEINITKNIPFYI"
     gene            complement(17168..18655)
                     /locus_tag="PLF48_00400"
     CDS             complement(17168..18655)
                     /locus_tag="PLF48_00400"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNF47807.1"
                     /translation="MKKVIAYLVIGMVSALFTLLIVNKFNYPGKTVLKESNGSPFKFA
                     SYNGSLPNDAFVQAAKVSTPAVVHINTKIKISKKDMREMNPFYQFFGDQFGMPYDGQG
                     QQDQEASGSGVIISNDGYIVTNNHVVEGADEINVSLNDNRQFKAKVVGTDPNTDLAVI
                     KIDAANLQFLTFANSDNVEVGQWVLAVGNPFNLASTVTAGIVSAKARNINILREKAGN
                     LAIESFIQTDAAVNPGNSGGALVDLEGNLIGINSAIATPTGSYAGYSFAIPSNLVKKI
                     VRDMIDFGIVQRGFLGVNIREVDDELAKDLKLSKIQGAYIAEVNKASAAESAGLKRGD
                     VITKVGVNEIKNTSDLQEHIAQYRPGEKVTLEVFRDGAYINKEVILKSKDNKTELLTK
                     TDEAAKKSGSVLENLGIEVAAVSGLEAKKLGIGGGLRITKLNDGILKQNTNIQEGFII
                     TGVNNQAVSSKEDLADLVEQSQGNGILLQGKYPNQNGIKYYAFGY"
     gene            18796..19590
                     /gene="dapF"
                     /locus_tag="PLF48_00405"
     CDS             18796..19590
                     /gene="dapF"
                     /locus_tag="PLF48_00405"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNF47808.1"
                     /translation="MIFSFSKYQGTGNDFILIDNRENIFPKNQHIIQQLCNRKFGIGA
                     DGLMLLENAEHYLFKMVYYNADGNEGSMCGNGGRCIVAFAERLGVIANQGTFIAVDGE
                     HDFELENNLVKLKMIDVHEIKNVGKHDFELNTGSPHYVVFTNDVDNLDVFGEGRKIRY
                     NETYQKQGINVNFAEITKDKIQMRTYERGVEDETLSCGTGNVAVALALASIQPQYTNK
                     ITLESPGGKLSVYFKQQEHSFSDIWLEGPAEFVYKGEIEIKEVARD"
     gene            19583..20533
                     /locus_tag="PLF48_00410"
     CDS             19583..20533
                     /locus_tag="PLF48_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNF47809.1"
                     /translation="MIKYYVKKGTTLEMTDKACSNCWINIVPPFNMDRLEHLSTELEI
                     PYSFIVDCIDQYERSRYEKEDDVRLIVINTPILNEGFDAEDEATYITVPVGIILVKDI
                     IVTVCSVNNPMIEWFEKNLLKNLSFEDRGMFVLRIFERNVHYFLYYLREINKRISQIE
                     KELNYSSRNAELNKLLHLQKALIYFVNDLRANEMMQMKVHRTNFLEVKDNEDAMELFQ
                     DVMIDNSQALEMANVYSNILGNMMGAFASIISNNLGHVVNRLTAVTIALMMPTLIAGI
                     YGMNIDLPFQHSPHSFLIVSLICILSTVVLVYYFRVKKWL"
     gene            20524..21588
                     /locus_tag="PLF48_00415"
     CDS             20524..21588
                     /locus_tag="PLF48_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNF47810.1"
                     /translation="MAVTDDKNLTTIRNILMFFASIVMMYLLYLLKDILIPLFLAMFL
                     ALLFQPILAAFERRKVPMIAGVSIIWLIFIIAMAGIGTIIYKTGMDLYHEKAVFIANA
                     KDKLIDFIDLYKRFTNRELDFEATVDNIGEFLRSQLTMENSTSYLGAIGMLLEEFLLT
                     AVFLAIFLSGIMRYENYLHYLGGQDGGGKFIHAFEQVKNAIVQYVKVKIVISTLYGIG
                     VAIICKLFGLQVAFFWGFIGLVLNFLPVIGALIGLVPVFLMAMIQYDSMATPWILIFI
                     CYAYHFILASLIEPFFMGAKTSLNVIAVIVGLLFWGFIWGIYGVFLSVPLMVLTKVIL
                     SQIEGAEVIVRLLGSQKDAK"
     gene            21777..23288
                     /locus_tag="PLF48_00420"
     CDS             21777..23288
                     /locus_tag="PLF48_00420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNF47811.1"
                     /translation="MLFNSLHFVIFFPLFLCAYFLVPRKDQYKVMVLGGYYFYGSFNW
                     YLLLILFGITCNDFFAGKMIEKTTEPHKRKRWLMLSIAGNLGVLIACKYLNFIDDNLR
                     EIFAFFHINWPIAEVAMNDWIVPVGVSFITFQSLSYTIDVYKGLIRAEKSFGHFCAFT
                     AFWPVMVNGPIERGKNLLPQINCEHAFDVFRLKEGLLRMAWGMFKKVVIADRLAFYVD
                     DFYTHYQVVSGWTIWIGGFLFLLQVYCDFSGYSDIAIGCARILGIDVMENFKRPLYSK
                     NMSDFWTRWHISLSTWLTDYVFYYLGAYKAGSLKVVFNVIFVLTLCGLWHGANWPMIL
                     AFTLIGIFMAIRFLWQQYIVRQIKPSNLYKISDKYIPSFMHVILTISIFIFCFILFRV
                     NSFEVTYNKLHAEAPLSWMEIAKPLYQHMFNLTSATYLLPWLNFKGAVTLGILIFYIL
                     ILLASEYVIGDRKIEEVVLPKSNLIRWSVYLFLLFSIVWFGVFNNTTFVYFQY"
     gene            23291..24355
                     /locus_tag="PLF48_00425"
     CDS             23291..24355
                     /locus_tag="PLF48_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47812.1"
                     /translation="MLRKQIKNSIVLFFALWLVSLIMSFIFPFYWGESTQATKFEYYK
                     KNKEKFNAVYLGGSLEYRHINPATVDSICKLNGLDFHSFNMASDGHNIIQEMTDLDGL
                     LAIDNPKLKYIFISISSEPYFFKANLHTSKWISWHTAKSTYRALRIIPTLKDDTKTKA
                     QYCIYYPMSWMENFFKFGNMTDALTYLKNKSHPDLAYLGPRDNGFYPYDLEANHTVVE
                     DPNNDKLIRASHDEFVRNKTKRDSLTKEILKSFSQYKSDDKPNKAEYNLLMEAYKKCA
                     KKKIQLIFMLPPKARTSYSFLLPLFHAMPEGTKIELADPRKYPELYQLEYGYNFHHLN
                     NKGATLESKILGEQVANLLK"
     gene            complement(24369..25457)
                     /locus_tag="PLF48_00430"
     CDS             complement(24369..25457)
                     /locus_tag="PLF48_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNF47813.1"
                     /translation="MRYGFILICVCALYLHINGQARFTLNDFYAENNDLDNLVDSAFE
                     KLTDKEKAAQLIMYAVSSTDNVYTFTKTKKLLQDTIISNVVFLKGQLDVIKNQINQFD
                     SLQQHLQNLYACDCEPSLFNMKYFGSSKVPKTNLLIYDSLLRQYTDTIIATLRRTGIQ
                     INFAPVVDKAFNTAVINNRSFGKNDSAIIHLSNQFISQTQEQQIATSIKHFPGHGNVT
                     GDSHKNLVYIDGALKEIQNFDSVYQASKPIFTMVGHIAVKNNQDWNTFNEPSTISANI
                     VNGLLKNKLQFKGIAVTDAMNMLGVATIPDADFKALKAGIDMVLMPVNPRKLHQQILD
                     EMKKDSFLSKQINASIKKIIRLKLCLTE"
     gene            complement(25459..25905)
                     /locus_tag="PLF48_00435"
     CDS             complement(25459..25905)
                     /locus_tag="PLF48_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HNF47814.1"
                     /translation="MLSKTLAELFERDLLKLKEEILLYKDEKKMWLIENQISNSGGNL
                     CLHLIGNLNHFIGATLGNSGYVRQRDLEFSSKDVPRATLIEKIEATIQVIVPILNQLS
                     QEQLEADFPLEKHGKIVTTEYMLLHLLTHLSYHLGQINYHRRLLDV"
     gene            complement(25915..>26248)
                     /locus_tag="PLF48_00440"
     CDS             complement(25915..>26248)
                     /locus_tag="PLF48_00440"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNF47815.1"
                     /translation="RMIICTPLATTDLVIDNIRNGIKDSVGSSHYAELKEPVEQITST
                     LKEIGIGYKDIVDIYLTSKGELLLYKNEKQEGKLKNGIGFAQALFEMYVGKRPKDKKI
                     KQALLTGF"
BASE COUNT         8392 a         4651 c         4633 g         8572 t
ORIGIN      
        1 tataaaaaca ggctattaca agctgatatg ttgttaaaaa tccgtatacc gattaaagat
       61 attggtgcgg aggcttatgc caaacaacag attttgctct ttgaagtatt tattttcgcg
      121 gtagcggaaa ttcaaaaaag cttctgtcga caaatttatt ttcgggttaa ttaaataacc
      181 gcctctggct tctacactat acatcctcgt tttcacgcct tgcaaaaatg ttttgccata
      241 agcctgattg aggttggaac ccagcatata atcagagata aaaatattgc ttccattatg
      301 cgcaaagggt tcatcaagcc cctgaactaa atactggaat tttaaatttg ctgtccaatg
      361 cttataccaa taatttacaa aaaccaaact ttccataaaa tttgctcccg ccgggtgtgc
      421 cagcgattgg ttgaaattgg catagttttg ctgtggcgat ttgtgtgcat acacgtatgg
      481 tcttactata tttaattccg tttgaaaatc gagatgtttg agcttgaaga tatcaaaagc
      541 tttatagccg atttgtgcag ctatttttgt acggtaaaaa cccttgcccg ctcgggcagc
      601 ccgcacatcc atgtcgtcaa acaaaatttg tgtgtaaata tgttgtttgt cgtttggatt
      661 aaatttcata gaaataccaa atgacacgtt atcaggcgaa ccgagtgcat attctatagg
      721 ccgcagaaat gccaacggaa tcaaatagga aaactccatt cctcttgttc tcaacgaatc
      781 ggcatgatac catactacgc tttcaaaaaa tccaaactgc atccatttgg caacatcaac
      841 actcagataa tgaaatgaac caaattttgt tttgaagttt ttataattat tctccgtatt
      901 aaatccgtaa gtatcacccg agcgcatgcc agccagtaag taggtatatt ttatacgcca
      961 aaactgcgtg gacattttta aatatggaaa attagacgca ttatcactca aaaacaaaga
     1021 acgatagcca tcgccccaaa atactttatc gttgcccagc tctaaattga catatttatt
     1081 aggtgcaaat cggatatagc cactcatcat atctgatcgc agtccgtttt tgccgaaatg
     1141 cgctttgcga tagcctgcca atacaccgta tttttgggca taagcagtaa cttgattatc
     1201 cattttctca aagtcgcctt gataggtaaa tgacaggctg acttttctgc ccagattcgc
     1261 ttcaaatttt gccccataac ctacggcata ttttagtttt ttgtccaaga catcgtatcc
     1321 tacttgtgca tcaataatcg ggtttaaggc aaaataaaat ttcttctttt tagcagatac
     1381 tgataaaaaa tcacctttcg ttaagcctaa tctgaattta tctgtcgagt ctgtaataaa
     1441 acccaattcc tgcaaggcag aatccggtat agctttaaga atctcggaca tattataagg
     1501 cttcacagca gtatgtacac cggagcttcc tgacaaatat ctttccagtc tgttttgcat
     1561 ctcgtagttg agataataat gcaattgctg agctgaagcc aaacaaagac agacagaaaa
     1621 aaagataagc gatagaatgc gtttcatgaa caaggctcaa aaataaagtt ttatgtaata
     1681 ataaagaata aaaggctcaa aaacttagga ttacttttat tttcgtcgtt cagactgtta
     1741 aaatatggca aaaggatttt cactcaaaaa tgatatcaag aaaattaact ggtatccgcc
     1801 ttatttaggt gccggtattt cggtaaccgc agtaaatgac aattttacaa gaatagaagt
     1861 tcagctaaaa agcacctggt acaacaaaaa tttgtttggt acacatttcg gaggttcatt
     1921 atacagcatg tgcgatccgt tctttgtatt tatcatcaca cagtatttgg gcaagggcta
     1981 tattgtatgg gataaagcag caagtattca gtttaaaaaa cccggcacag gcactgtaaa
     2041 agccgttttt gaaatagagg aagaaacgct gaagaaagta aaatccgaaa tagatgagtt
     2101 aggcaagaaa gattatccat tttcagccac aataaaaaac agcgcagatg aagtagtggc
     2161 agaagtggaa aagttgattt atgttcgtaa aaagtaaata tcccgctctg tttgttaata
     2221 ctgtttttaa aagttaattt catccttgta agatatatag gtaataaaaa tgggaaatgt
     2281 ttcgtgtaaa atttgtcagt caactgctac aaatatcttt ttgttagatc aaacatcatt
     2341 aatcaaatgt gagaactgtg gtattgtata taatcagcac acttcgagcg aaaaagaaac
     2401 tacttattac aaagaaaatt acaccttgcg taaaaacaat atttcactcc aaagtgaatt
     2461 gcgacgtttt tcgagatggc cggaacaact caaactcctt gctgatataa aaaaaacaaa
     2521 gcccgaagct gcaaatatta ttgatgtagg ttgtgataag ggctatttta tagatgaagc
     2581 acgtcggttt ggttatagtg tttatggtgt ggaactttca aaagatgcac gaaaatatac
     2641 cgaccaactt gggcttactg ttaaagattc tgttgaagaa attggtgaaa aggctgatat
     2701 cattacgatg tggcattctt tagaacattt ttcagagcca ttatcattct tgaaaagcct
     2761 gcatccacat ttaaattctc aagctcatat atttatacgc gtccctgatt tcggctgttt
     2821 tttcagcaaa gtattaaaac aaaaatggcg ttggtttcaa ccagaaaacc attatttcca
     2881 ttatactgaa aaatctttaa aacatttgtt tgaagaagcc ggatttcaag ttgtgcaatg
     2941 caggtcgcag aacccgagca gttgcttaac cgacaaggca ttttctttag ccaacaaaac
     3001 tttgcgaaaa tactacaatg aacccgcatc gctaaaatat gtactgggaa cctattatga
     3061 acgcatcacc tgtaaagagt tgtatttgat tgccaagacc tcgtagtttg gcaatccacc
     3121 tcgtaggtcg aatttttaaa tccttaataa tcagccgagt ataaaattca gtatgcaaat
     3181 tgacctcgta ggtgttgctt gcagcttgaa cataattccg ttttttcatt atcaatcgtt
     3241 gatatagatt cgttagcatc gttcataaaa caagatttag acggacaatg aggaagtccc
     3301 agattatgtc ccaactcatg aagagcaatt ttttctaatc gttgagcgaa taatgcttta
     3361 tctgaagatt ttatcctgaa tgtagaaata acagctgcgt ttccggggcg gaaagccaag
     3421 cccataatcc cccaatctgc atattttgtg gcaggatttt ttattttgcc tgatgcatct
     3481 tttttggtag ttgaaatatc tgcctcggta atgcccatta tataatttac tgaatcaggc
     3541 tttatttttt gcagatatag aagaaggctg tctgcacggt aacgcggaga ttttactggt
     3601 gtgaatgcat ttggaggaag aacagtttct ggcaacaaga cgacattaaa accataatat
     3661 ttttcgagtg cattttttac tgtaatcata tttgcaagct ttattttccc gagtggcaat
     3721 aagcctaaat taacttgaga gcaattttca ggatgattgc aactcagaca aatgaataaa
     3781 gaaaataata taactatacc tttcatttag agtgaaagtt aggaaagatt ataatagtga
     3841 agcgttaaag agtgtgatat tctaaattaa acctccacaa caaatcagca tcagctccgg
     3901 cgtagtctat acctattctc tttgaagtag taattaaacc gtcacttagt ggataatcat
     3961 tttcttgcaa ccaaatggtt tgctcttgca gtagcgtctc attttgtgat ttagtcaagc
     4021 ctagcgcttg agccactttg gcaggacctt tgcatatatt cttatttaat ttagtttgat
     4081 gcctgcgctc aagcatgact gatatgccaa tagtaggata tatcgctctg attaatacgg
     4141 cttgtgccac atccttttta cccgttacta tatttaataa ataatgcata ccgtaacaaa
     4201 gataaatata agcatatccg ccctgctgaa acatgatttc cgtgcgccgg gtgcgtttgc
     4261 cgttataggc gtgacatccc ttttccgact ggcagtaggc ttctgtttcg gtaattattc
     4321 cggcggtaat ttggttatct atatttgtaa acaggatttt gcccaataag tctttagcca
     4381 aaaaaacgac atcatcgtta tggaaataag ataaaggtaa tttcatatag atgcaaaata
     4441 actaaataac ctcgtaggtt gggtatactt tattaaaaaa gtattgattt tattggttgg
     4501 cggcggcgaa gccgccgcca accaacctac gaggtcggat tttttgtaat attgcgaaat
     4561 ggcttacggc aaactttttc tgatacccaa cacactgcat gaaggcaact atgtaagcct
     4621 gatacatctc gtcccgataa tagcatcaac ggatatttat attgtcgagg aaataaaatc
     4681 agcacgaaga ctccttaaag gtgttttgaa aacaaaagat attaattcat gtacttttaa
     4741 gatattagac aaacacaaag actatcaagt gcatttttca attttagatg aaataaaaca
     4801 agataaaaac ataggtatca tttcagaagc aggtctgcct tgcattgccg accccggatt
     4861 tcaggtggtg caactggcac acgaaatggg cgttgaagtc gtacctgtat ctggtcccaa
     4921 ctccatgatg atggcattaa tggcaagcgg tttcaacggg caatctttta cgtttaacgg
     4981 ctatctgcct attgaacaaa cagagcggaa aaataagatt attcagcttg aaagttttgc
     5041 taaaaaaaat catacccagc tctttatgga tacgcctttt agaaacaaca agacacttga
     5101 agaactctta aaatattgca aaccggatac acttttgtgt attgcctgca accttatggc
     5161 aaaagatgaa tttattcaaa cacaaactat agctgactgg aaaaaaataa aacctgattt
     5221 aaataaaaag ccttgtattt ttattttggg aaaataaata acttatgatt ttaatagacg
     5281 atatattaat cagtgatgat gtagtggaaa aagagtttgt ttgcaacttg aaaaaatgca
     5341 aaggcatctg ctgcgtagaa ggcgacagcg gtgcacccgt ggaaaaggcg gaaaccaaaa
     5401 tattaaaaaa gattttcccg aaaataaaaa aatacctgac cgctgaaggt atcgcggcta
     5461 ttgaaaaaca aggtacatac gtggatgaac ccgatgatga atatacttcc tttgcaacac
     5521 cactaattga cggtggggct tgtgcttata taaattatgc cgatgacggc acgatagtat
     5581 gtggaataga aaatgcatgg cgtgatggcg ttgtggactt tcagaagcct atttcttgtc
     5641 atttataccc tgtacgcatc aaaccgatgg aaaccgtaac ggctgtaaac tacgatgtat
     5701 gggatatctg cagtgaagcg tgtttgttgg gtaagaaatt aaaagttcct gtttatgtgt
     5761 ttttaaaaga tgcgctgata agaaaattcg gagaagactt ttatgcggca ctggaatatg
     5821 cagcgaagga taatgaagta taaaagaaaa gaattatttt tggatgtact taataatatc
     5881 aattcagatg gcattagtgt acgcaaccta ttattactcc aaaatcaccg tcagtctgta
     5941 tccagttttt ttcgggcaga tacattcttg aaacatatcc atccagatac aaggcttgtg
     6001 tacatccagc ttgtttgaaa aatgaagcaa actcatagaa attaattttc tgttttgaga
     6061 ttgcaaagat tactttatta tccggcagca agccgacacc atttcggata tataaacttg
     6121 tagattgtat gttaaataca ggatttattt ttccatctat tacaagcatc ggtcctgact
     6181 gtgtagcgta acgtatagat gtggaaaaac gaaaatcttc agttttacag agagcgggct
     6241 tattatcatt cattaaataa aatataccgt ttggttttaa atagaaattt ccatatccta
     6301 tatccgtatt caatgatttc acaatacgat ggtcttgaat aaataaacct tgtggtgaat
     6361 tatcttgcat atacattcca ccattcatgg caaaacgcaa tccttcatct ttttgttcta
     6421 catcaaattt aagattattg atactcctgt acaggttacc ggatttgtct ttgtagtaaa
     6481 aacggatact atctgattgt ggatttaatt tcacagaaac tattttttct gattctttac
     6541 actcattttt ataaatataa gccgaagccg gaatcagcaa tagtaagaat ataaaaatca
     6601 gaagtttata gttcatcgtt aaactttgcc cactcaccaa ctgccaactt aagttacagt
     6661 gttttgtaaa acttgctcaa tcgtgcttct atgcgctctt gcagaaagcc gtctttacgc
     6721 gctttctcaa ttacaggtat agcaaattct tccgtttctt tgataacttt ttccatccta
     6781 actccattct catcgagcaa ttggacaata tttttatctc caacataatc aaagaaatgg
     6841 tcgagtacaa aatctgaaat ttcttccgtc gttgcatttt ttaatatatc tcctgcaact
     6901 tgttctgcaa tatccaagcc ttcgttgatg tctttggtct tgattttttt ggcataatca
     6961 ctaattttca catcatgaat tttattccaa atggtcttgg acatcagtct gagtttgggc
     7021 tcgctaaaac ggtctttcag gttttgctca cttttttgca aggttttaga aagattggcc
     7081 gcgataaatt ttttcacggt cgaatcaata ttatcttgca caccggagag tgcggcaccc
     7141 agcaatcctt taccaatatt aaagagggag cccccaacgg attcgttact cggtccactt
     7201 tgttgcatga attgcttgat gccgtcatac aaggtatcgg ctattatttc tccataaaca
     7261 ggattgtgag ctattctctc gatgagttct tcgcgccatt ctttttgcgc tatcagttta
     7321 tcagcgattt caaaaaaacg tttttctgta atataattgc ttaccgtgtg ttttgatttt
     7381 tttgcatgtt catgtatatg cttggccaag tctttggcat aatcgcgctg ctcatcagaa
     7441 agttcatagt gttttacatt tcttttcaaa aatgatttta tgtcttcagc cgaagtaata
     7501 tcgtttaaag taactttttc cagccatttg aatacagcat gaacatcctc ttttatattg
     7561 gatttaatgt gttttttacc aaattgttca agctcaaact ggacgtgggc atcatagatt
     7621 tttttaatct ctgctttcat taagtagaat tttttatgaa gataactgat atcgctaaac
     7681 aaaagaaatt acgaaggata atttaatgtt tagctctaag ccggctgagc gtttcttgcg
     7741 taataccgag ataagaagaa atatagcgaa gaggtatgcg attgagtaaa tccccccttt
     7801 cttttacaaa agtatgatag cgttcttcag cagaaagaaa cagtacttca tacgattttt
     7861 tcataaataa ggaataataa ttttcaatta gcttgcgacc gaaatgttcc atacaatgat
     7921 tctttgcata aaactcttct aattttctgt aattaagttt caggcaaatg gtgtcttcaa
     7981 gggtttcata gtaattgtaa tttcgctcac cggtgagaat ggcatacaca gctgcaaaga
     8041 acatattttc actgataaac cagtttgtaa tttctttttc ttcccgcaca taataaatac
     8101 gtacgagtcc ttttataaca aatcgggttt cttctgaata ttcaccctct ctgaggataa
     8161 atgtcttttt gggtataaac tccaactcga aagcttttac gaattcatct aaattctcag
     8221 gcttaatatc agcaatttgt tgaattattt ttttcaactg ttcttgatat acttctatgt
     8281 tttttgttgg tttttgcatg ctaatagctt taagatcatg aatatttggc tcttaatctg
     8341 ctgagcgttt ccggagtcaa tcccaaatag gaggctatat atttgagctg tactcgctgt
     8401 aataaggctg attgtgtttt caaaaatcga atataccgtt cttcagcact caaatataga
     8461 atattattat tattatataa tgcattaata taattttgtt ctaccacttt ccgtcctaat
     8521 gtttctaacg catgatgctt cttatacaag atttccaatt tatcataatt gaagactaag
     8581 caaactgttt tttccagtgt tgtgtagtaa taaaaattct ggatatgccc atataaataa
     8641 tttgaattcg taaaaaagtt gtgttcgctt ttaaaggaga aagtaatgtc cttatcttct
     8701 tttgtataat gtacacgaac caatccattc agtataaaat atacatccct aaacacatct
     8761 cccggaagta ttaaatccgt atttttttct atttctacga ccatacaaag agctaagacc
     8821 tcctctataa cctcatcatc cattttcacc acggcattcg ccacatgtcg cagaacattc
     8881 agctggtttt cattaggctt atatgatttc atagtatgca aatatactat atctcttgat
     8941 tcaaatcaaa tttttctaaa taatctgcta ctttttttac aaataatccg cctaacatac
     9001 cgtccactac cctgtgatcg taagtatgag ataagtacat catgtggcgt atggcaataa
     9061 catcttgtcc gtcaacttct aatacaaccg gttttttggt aatagcacct actgccatga
     9121 tggctacctg tggttgattt atgatgggcg tgcctataat atttccaaaa gtgccaatat
     9181 ttgagatggt ataagtgcca tctgcaatat catcggattg caacgagttt gttcttgctt
     9241 tattaaccaa agcattcatg gctttagcaa tacccaataa acttttttgg tcggcttgtt
     9301 ttaccacagg cactatcaga tttcccgaag gcaatgccgt agctacacca atattgatat
     9361 ctcttttttt gataatctta tcaccatcca ccgatacatt gatcatcgga taatctttaa
     9421 tggctttcac aatagcttct acaaaaacgg gcataaacga caagctgaat ccttcgcgtt
     9481 tttggaatgc gtttttattt ttatctctcc aacgtactat attcgttaca tcacattcta
     9541 caaacgaaga aacatgtgca gaagttttct tcgaattgac catatgctgt gcaatcagtt
     9601 tgcgcattct gtccatttca atgatttctg tattcggttg ttctgcattc gtttgtttag
     9661 aatctttctt ttgtccggtt tcagttgttg aagttgcttc ccgtttttct ttctgaacag
     9721 taggtatcac aggagttaca ataagtcctt ttttcttttt atctgcatat tccagtatat
     9781 cttctttagt aacacgtcca tccatacccg agccttgcaa agcatctaat tcggtcatgc
     9841 ttatttgttc agcttgtgca atgctcatca ctaatggaga ataatatttt gcagaactac
     9901 tgataacggg tgctgtatat tgtacgctaa ctttagcttg ctctactgta ttttcaacgg
     9961 ctttaatttc cggaattgca ttttcaactt tcagttcaat gtctttaaac tccggtggtt
    10021 ttatttcatt tttagtttcc accacaactt ctttatctgc cactttattt gatggtgtaa
    10081 cttgaatttc actgccttca atttcaatga tggccaacac atctcctact ggaacaacgg
    10141 catctttatc gtgcaaaatt tgttttaata cgccgctcac aggactgggc acttcggagt
    10201 ccactttatc cgtggctact tcaaccaata catcgtgttc atttatcttg tcgccgggtt
    10261 gtttatgcca ttgcaaaaca gtagcttcaa ttacactttc gcccatcttg ggcaataaaa
    10321 atttatattc tgccatatgt ttttataatg agtacaagat acgaagtgaa taagaaattc
    10381 tatgaataat atttcaacac aaattttcgc aacatcacta atgccacgac actacttagt
    10441 tcaatgttcc tgcccctatg gttcgtgaat gataaacggc gaatgtattg ctgattttta
    10501 tttgcgatac ctatcatgac tgttccaacc ggtttttcag cagagccgcc accgggacct
    10561 gccactcccg aaacggctat tgccaaatcg gtatgcagaa cagataaggc accgtctaac
    10621 atttctgcaa ccgtttcttc acttactgcg ccaaaacgct gaattgttgc tgattgcaca
    10681 tgtaatactt gctctttcac atcatttgaa taagccacca cacttccttt aaaataagca
    10741 gagctgcctg caacggaagt aatcatttgt gcaatagtac caccggtgca actttcggct
    10801 gttgaaaggg ttaatttttt atcctttaat aatttgccaa tatttgcttc taaagtttct
    10861 tcatcaaagc cgtaaacata ctcaccaatg gctgccaaca cctcattccg ggattgttcc
    10921 atgagttcgt aaagtgtggt attttgtatg gcatggtttt cagcttcact ttttatagtc
    10981 aatcgcaact ttactttgcc tacagacggc agatatgcca acttaacttg tggatgttta
    11041 tttttttcaa atactgttaa acgctctgct aattgcgttt cgcctactcc ggcggttagt
    11101 atagttttat gcaaaatcaa aggcaagcta aaattttgct gtaagtatgg aataaaataa
    11161 tccgttacca tagcctgcat ttcataaggt acgcccggca tagaaaccac cactttattt
    11221 tcatttctga agagcattcc tgcggcggtg ccgttcttat taggtatggg tatgcataca
    11281 tccggcagaa aagcaacttg tcgggtagct tcatttattt ctcgctggcg caaagaaaaa
    11341 atgcgctcaa tattttcata agctttttca ttgaaaacaa gcgttcctcc aaaataatca
    11401 cacaatactt tcttggtaat atcatcagaa gtgggaccca atcctccggt aattaaaatc
    11461 acatctgcca aagctaatgc ttcatccagg gtttttgtaa tactgttttt ttcatcggca
    11521 atagatatat gccgaataat atcaatacct gtctcattca ttttctgtga aataaaccaa
    11581 gcgttggtat tgagtgtctg accaatcaac aattcatcac cgatgcaaat aacgatacct
    11641 ctcatcttag gaataatttt tgtattatta cacaataatc tgaatttatt ttttatccaa
    11701 caacaaaatt attcgtatga aaaaagtatt cgtcatcctg ttaagttcat ttcttttatt
    11761 cggatgtaca tcacaacaaa tccaggatac tgtaggttct attttaggat acagcggtac
    11821 cccaactgat gcagaagccg gaaacggctt gaaagaagca ttaagtattg gcattgtaaa
    11881 cggaatggga ttattgtcga agactgacgg atttttgggt aatgacttag tgaaaattcc
    11941 gtggcccgat caggcgaagc ctgtgatgga tgctatgctg aaactgggca tgcaaaaaca
    12001 agtggataat gtaaccacgt cattaaaccg tgctgccgaa caagcttccg gtgttgcgat
    12061 agatgtattt aagcaagcta ttatgcaaat gactattact gatgccatga atattttatt
    12121 gggaggtaat ggttcggcta ctgcttacct gaaaaaaaca actactccta tacttactga
    12181 aaaatttaga cctattgtcg ataattcttt aggtaaagta aatgcgacaa aatattggag
    12241 tgatgccatt actgtttata ataaaattcc atttgtaaag cctgtaaata ctgatttaac
    12301 tggttttgtt acggagaaag ctttacaggg tgtgtttgtt atggtggaaa aagaagaaaa
    12361 caaaatccgt gaaaatcctt tggctcgtac cagtgaatta ttgaaaaaag tattcggatt
    12421 tgcagacacg aagaaataga aatctatttc aaacctgtta cttacataca actacttaac
    12481 tattaaacta cttaactcat aaattcttat gagcagaaaa ccacaacaag ggcctttaca
    12541 aggtattcgc gttttagact tatcccgttt gttgccggga cctttagcca cacaacttat
    12601 ggcagacatg ggtgccgaag tcataaagat agaagacccg aacgcccagg atatgacgcg
    12661 tttttttccg cctatgataa aaaaccaatc cctgttttat ttgagtataa atcgcagtaa
    12721 actatctgta acattgcctc ttaaaatgga ggaaggtgta caacagttta tggagctggt
    12781 gaaaacggca gacgtagtat ttgattcctt tcgtccgggt gtactggata aaatgcaaat
    12841 cggatatgaa gatgcaaaaa aagtaaaccc caagataatt tatgttgccg taaccgggta
    12901 tggacaaaca ggcactatga aaaacaaagc cgggcatgat gttaactatc tgggctattc
    12961 gggtatttta ggtatgaccg gattaaaaaa ccaaccgata atgccgggtg tacaaattgc
    13021 agacaccgcc ggaggaagct atgcagccgt tacggcatgt ttagctgcat tgtgggcacg
    13081 cgagcgcaca ggaaaagggc aattggtaga tgtggcgatg accgactgcg tgatgcccat
    13141 ggcatctatc ctctatacga agaaactcaa cagtgatgat gattcattgc gtcgtggatt
    13201 tgattatttg agtggtgggc tggcgcatta taatatttat caatgtcaag atggaaagtg
    13261 gatagcctta ggctcattag aaccaaaatt ctttcaagga ttttgtatga tgataggcaa
    13321 accggactgg gtaatgcgca tgttgccaga agaaaaaacg gtagaaagct tacgcgaaga
    13381 tttagcagca ctctttctta caaaaaccca agcagaatgg ctgcaaatgg ctgcaggtca
    13441 ggatatttgc ctgacgccca taatggattt ggacgaagta tataacagtg attatgccaa
    13501 agaacgggag ttgttttttg acatggaaca tcctgcttat ggaaccgtaa aaacagttaa
    13561 tcagccgttt aagttttctg aaacgcctgc gaaaccgagc tggggtgcgc cgttgttggg
    13621 cgaagacaat gaaatgctta aataggttct tcctaacgag aatatggatt tataaatttt
    13681 ggattttaga tggatgagta gatccataat catttattat tgcatagcag acctataatt
    13741 attacagatt atctttttat tggtttattt tgatgttttt ttagttaaaa ataaataaaa
    13801 aaagcattta attacactaa atgagtatat tggttgttta atatacataa acttttataa
    13861 cacaacttat gacaccagaa gaatttaata gaattacaaa acaatgccag ccacacaaag
    13921 tatttaaatt agattggaag acttattatt cggaagactt tggtaacact ttcttgcctg
    13981 acatagaacc gattggtggt aaagcatttt tgatgtatga tggtgcagat ttctttagaa
    14041 acctcattcc aacagagaga aaacttttga aacttacaag aggaacagaa ctaatttcgt
    14101 taacatttta tacttaccca aaaggtgtat tggctcaaaa aagaaaagac agagtaagtg
    14161 ttgaagattt tgaaatttgc gcatttattg aagttgatga aaacgataag ttagaaatat
    14221 tttttaagtt tggcagaaat tttaaagatt tcttatataa atattttaca gaaaataaga
    14281 ttaacaaaaa tcctgaagct gttgaaaaaa ctatatataa aagcaacact aaagaattaa
    14341 tgggttattt caataatagt attgttcatt atagtttaat ggcatatgta ggcaaaacta
    14401 ttggagttga tttagtagct gtaaataaaa aagtggcgta cgaattaaag ataaataaaa
    14461 ttagtattag ctcatcaaca ctatcaggaa atctaaataa acatattaaa tcagacaaag
    14521 aggaatatat aaatatagat tttacaatat acaattatcc atctacagaa tacagaataa
    14581 tcatttataa aaataatgaa aaaattgacg aactagtatt tttacctgag attgtaatta
    14641 taccaaacaa agaaaaaata gacacacaaa cagaaaacac actatacaaa gcagggaaac
    14701 atcgaattgt atatgaatgt agcaatacag atattctaat tggaaaaaat gagaacgata
    14761 aaataacgtt acgaatacaa gctaccaatg aaaataaaga aatagcttac gaccaaaaag
    14821 aattattgtt gaaaatagcg atgcaaaaag cagcaactaa caactatatt ttattggttg
    14881 accctaataa gaaagaagga gagaaagatt gctacataaa aatatatgcc aacgaaattc
    14941 taaaaaaaca aaagataata aaaaataaaa ttccaaaagc agagtataac aaattatcag
    15001 tagaagaaaa attaattatt gatttgccaa taattttagt aaaattagat tggttgtata
    15061 gcgcatattg tcaattatat tggttagaag gaagtggtga agatatttat ttgcctataa
    15121 gttttgtaga aagttatgca agagttcaag atgccttaaa gcaaatgaag aatgatttct
    15181 cagaaattga atatgcatcc tatatggatg agacgaatta taatcctaac cttagtattg
    15241 atgatagtag taatagattt ttgtataaca tcagcttaaa tcaagtcaaa ttatcttata
    15301 ataaagcaac tgctattcta aataaaatta cagattttgg agaatacgac tttggtaatt
    15361 ttgatatgaa agaagctaat ccaaaattaa attttgtcag gtcgtatact attggttcac
    15421 atattggaaa agttgctaca ggtgacaagg atgatttagg tggtgcgtta ggtttatttg
    15481 ccttaagaat ctatataaaa ggtaaagtta ctaaacaaga atttaactca aatcaagtaa
    15541 gattatttta tttaaatata tctaatgtag gttataggta ttttgatgat ttttcttttg
    15601 atggatttca acctcttggt gcatggagat atgatttaga taatccagtg cctccagaaa
    15661 agctaactta taagagagac aaatggtatt ctttatggaa tgaaacatat cgaactctac
    15721 aaaacagaaa aaatacaaat ttatctccga aaagttttta tattattact ggtataaaac
    15781 aattaaaaaa ccacaatgaa attaatataa caaaaaatat tccattttat atatgaaacg
    15841 aaatataact tatgcaattg ttaagaaggt agttaaatca tttacataca tatttatttt
    15901 tatagttata ttgtttattg ccattaacat atatttgtct attccgcaat taacgagagt
    15961 atataatagt ccttataaaa atacagttat taaaagatac gatttgccag catttttatt
    16021 tttttataat atgtatccaa aaagttatta taaaatttat atacatagga aattaattgc
    16081 aaaagtggat gcaaattgct ttgaatgttt agaacacaac atactaaata taagatggta
    16141 taaagatagt gtaaatgtaa aatacgaaat acattatgaa aaatatgata ctataaatat
    16201 tacttacatt tatagtgata aataaatgta tgccacctaa aaatcaacaa taaaataatt
    16261 tgtttacaaa ataaagatta tcaggaattg catgatatat taaatccaag acaaattcca
    16321 aataatagaa ataatagacc aattattatt caaaatgctt gcagagattt tttaatttat
    16381 tgtgactata aaattttcaa tgatgaattt attcaaaaaa gggatatttc agtttgataa
    16441 tttgatataa tcactttacc atctaattct atgttgaatt caaatataaa gatatcgtca
    16501 ttttttacta aaccaaatct aaggttaata ctaagaaaag cgttgattat cttttttatt
    16561 ttttgttttt tattgtatat taacgaaaaa aacaacacaa agagagttta cttaaattta
    16621 agtacagtat tggtaggcga agatgttcaa acagcaataa aaaagatgaa acactgcact
    16681 tttttattgg agtataaaga tgaaccttgc agtaattctt cgaataacaa tgtatacaca
    16741 catgaaatta tttttaaaca taaagaccca aatgattatc caaatgatta tctaagatta
    16801 tattacgata tacgaacaaa tagaataact ggtaaatggt taggaaatat aggaagctag
    16861 aagtaacaaa actaaaaatg tttgctaatt aaaatttggc aatagtcgtg ttagatttgg
    16921 caattggtgt aaagtgtaat acatttaatt tgtaaatcaa taaaagtttg ttctaaatac
    16981 gcacaaccat catcaatttt cagccgttag attctgaatt ttggatgaag attgttttta
    17041 attacagttt caattaacag taataaatca catgcatttc aatggctctc ataaaaagaa
    17101 caaccaaaaa tttgctaaaa aaatggaagc ctttcggctc ccatcaaaca catcttatga
    17161 aaaactgtca atacccaaac gcatagtatt ttatgccatt ttgattggga tatttgcctt
    17221 gtaataatat accgttgcct tggctctgtt ctaccaaatc ggctaaatct tctttgctcg
    17281 aaacagcttg attatttaca cccgtaataa taaacccttc ctgtatattg gtgttttgtt
    17341 tcaaaatacc gtcattcagt ttggtaatgc gcaaacctcc gcctataccc aattttttgg
    17401 cttccaggcc actcacagct gctacctcaa tgcccaaatt ttccaacaca ctgccacttt
    17461 ttttggcagc ttcatcggtt ttagtcaaca gttccgtctt attatctttg gatttaagaa
    17521 ttacttcttt gttaatgtat gcgccatctc tgaatacttc cagcgttact ttttctccgg
    17581 gtctgtattg tgcaatatgt tcctgcaaat cagaggtatt ttttatttca ttaacgccca
    17641 cttttgtaat gacatcacct cttttcaatc ctgcactttc tgctgcactg gctttattta
    17701 cttcggcaat ataagcaccc tgtattttag acaatttcaa atctttcgct aattcatcat
    17761 ctacttcacg gatatttaca cccaaaaagc ctcgctgtac aataccaaaa tctatcatat
    17821 cacgcactat cttttttacc aaattagatg gaatagcaaa ggaataaccg gcataagaac
    17881 ccgttggcgt ggcaatagcc gagttgatgc caatcagatt tccttccaaa tccactaaag
    17941 caccgccact gttaccgggg ttcacggctg catccgtttg tataaacgat tctatcgcca
    18001 agttgccggc tttttcgcga agtatattga tatttctggc ttttgcagat acgatacctg
    18061 ccgttacggt agaagccaga ttaaaaggat tacctactgc caacacccac tgccctactt
    18121 ctacattatc cgaattggca aaggtcagaa actgcaaatt agcggcatca atttttatca
    18181 ctgccaaatc tgtgttgggg tcagtaccca ctactttggc tttaaactgc cgattgtcat
    18241 taagacttac attgatttca tcagctcctt ctactacgtg attattcgtt acgatataac
    18301 catcgttaga gataatcaca cccgaacccg aagcttcctg gtcttgctgc ccttgaccat
    18361 catacggcat gccgaactga tcgccaaaga attgataaaa aggattcatc tcccgcatat
    18421 ctttcttact tattttaatt ttagtattga tatgcactac cgcaggcgta gatactttag
    18481 cggcctgaac aaaagcatca tttggcaaac tgccattata cgaagcaaac ttaaatggtg
    18541 acccgtttga ttcttttaac acggtttttc ccgggtaatt aaatttattc acgattaata
    18601 aagtgaataa tgcgcttacc atcccgataa ctaaatatgc aataactttt ttcatacact
    18661 aactttttta ttgtttttac acccgcaaat tacagaccta ttgaggcaaa aatcaagtaa
    18721 agagatgtaa agatgggtta atatttgtta agctctttta aaacttagta cgattaaata
    18781 tactaccttt gcaccgtgat tttctcattc agtaaatatc agggcaccgg caacgatttt
    18841 atcttgatag ataacagaga aaacatattc cccaaaaatc aacatatcat acaacaactt
    18901 tgtaacagaa aattcggaat aggtgcagat ggtttgatgt tgctcgaaaa cgctgaacac
    18961 tatttattta aaatggtgta ttataatgcc gatggaaacg aaggcagtat gtgtggcaat
    19021 ggaggcagat gtatagttgc ttttgcagaa cggctgggtg taatcgctaa tcaaggaaca
    19081 tttattgccg tagacggaga acatgatttt gagctagaaa ataatttagt caaactcaaa
    19141 atgatagatg ttcacgaaat taaaaatgtc gggaagcatg attttgaatt aaatacaggc
    19201 tcaccgcatt atgtcgtttt tacaaatgat gtagataatc tggatgtgtt tggtgaaggt
    19261 cggaaaatac ggtataacga aacttaccaa aaacagggaa tcaatgtaaa ttttgcagaa
    19321 atcacgaaag acaaaatcca aatgcgtact tatgagcgtg gtgtggaaga cgaaacattg
    19381 tcctgtggca cgggaaatgt agccgttgcc ttggcattag cctcaataca accgcagtat
    19441 accaataaga taacactgga atctcccggt gggaagttat ccgtttattt taaacagcaa
    19501 gaacattctt tcagtgatat ctggcttgaa ggtcctgctg aatttgttta taaaggtgag
    19561 attgaaataa aggaggttgc acgtgattaa gtattatgta aaaaaaggca caaccttaga
    19621 gatgaccgat aaggcctgca gtaattgctg gataaatatc gttccgcctt ttaatatgga
    19681 caggctggaa catttatcta cagagcttga aattccctat tcttttatcg tagattgtat
    19741 tgaccagtac gaacgctctc gctatgaaaa agaagatgat gtcagattaa tcgttatcaa
    19801 tacccctatt ttaaatgaag gatttgatgc tgaagatgag gctacgtata tcaccgttcc
    19861 ggtcggaatt attttagtca aagacattat tgttacggta tgctctgtca acaatccaat
    19921 gatagaatgg tttgaaaaaa atctgttgaa aaatctttcc tttgaagaca gaggcatgtt
    19981 tgtattgcgc atttttgaac gcaatgtcca ttatttttta tattacttgc gagagattaa
    20041 taaacgcatt tcgcaaattg aaaaggaact gaattacagc agccgtaatg cagaattaaa
    20101 taaactactg catttgcaaa aagccttgat ttattttgtg aatgacttga gagctaatga
    20161 aatgatgcaa atgaaagtgc accgcaccaa tttcctcgag gtaaaagaca acgaagatgc
    20221 catggaatta tttcaggatg tgatgattga caattcacaa gcattggaaa tggcaaatgt
    20281 ctactcgaat atcctgggca atatgatggg agcttttgca tctattattt ccaataattt
    20341 aggtcatgtc gttaatcgtt taactgcggt tacgattgcc ttgatgatgc ctacactgat
    20401 tgccggtatt tacggtatga atatcgattt gccctttcag cactcacctc attctttttt
    20461 aattgtcagt ctgatatgca tactcagtac tgttgtttta gtttattatt ttagggtaaa
    20521 aaaatggctg taaccgacga taaaaatctg acaacgattc gtaatatcct gatgtttttt
    20581 gccagcatcg tgatgatgta tttgttatat ctgcttaaag acatcctaat tccattattt
    20641 ctggctatgt ttttagcctt gctgtttcag cccatcttag cagcttttga gcgacgcaaa
    20701 gtgcccatga ttgccggagt aagtatcatc tggttgattt ttattattgc catggcaggt
    20761 attggcacta ttatttataa aacaggaatg gacttatatc atgagaaggc tgtatttatt
    20821 gccaatgcaa aggataagct cattgatttt attgatttat acaaaagatt caccaacaga
    20881 gaactcgatt ttgaggcaac tgtagacaat atcggcgaat ttttgcgctc acaactgacc
    20941 atggaaaact ccacttcata tcttggtgcc atcggtatgc tgctggaaga attcttgttg
    21001 acagctgtgt ttttagctat ctttttatcc ggtattatgc gctacgaaaa ttaccttcat
    21061 tatctcggtg gacaagatgg aggaggaaaa tttatccatg cctttgagca agttaagaat
    21121 gctatcgtac aatatgttaa agtaaaaata gtcatcagta ctctatatgg catcggcgtg
    21181 gctattatat gtaaattatt cggtctgcaa gtcgcatttt tttggggctt tattggatta
    21241 gtactcaatt ttctgcctgt tataggagca cttattggct tagttccggt ttttttaatg
    21301 gctatgatac aatatgattc aatggccaca ccctggatac tcattttcat ttgttatgct
    21361 tatcacttta ttttggcatc attgatagaa ccatttttca tgggtgcaaa aacctcatta
    21421 aatgttatag ccgtcattgt aggtttattg ttctggggtt ttatatgggg catttacggc
    21481 gtattcttat ccgtaccttt aatggtattg acgaaagtaa ttctctctca aattgaaggt
    21541 gcggaagtga ttgtcagatt gctgggctct caaaaagatg caaaataaga ttcacatctt
    21601 gcaccacaac tttttcttgt aaaaaatcaa ctctttttta tccaatcaag gacattcaga
    21661 aaataagaat tcaacaaaaa cattgatata attcttaaag ttcatttcta aatcctattt
    21721 caaccggcat aacaaaatgc agtattcttc atatattcgc agttcgactc tcaactatgc
    21781 tgtttaattc acttcacttt gttattttct ttccgctatt tctttgcgcc tactttttag
    21841 taccacgcaa agaccagtac aaagtgatgg ttttgggtgg ttattacttt tacgggagtt
    21901 tcaactggta tctcttattg attttattcg gcatcacctg caatgatttt tttgccggaa
    21961 aaatgataga aaaaacaact gagccacaca aacgcaaacg gtggttaatg cttagtatag
    22021 cgggcaattt aggcgttttg atagcgtgta aatatctgaa ttttattgat gacaacctcc
    22081 gggaaatatt cgcctttttt catattaact ggccgatagc cgaagtagca atgaatgact
    22141 ggattgttcc ggttggcgtt tcttttatta cctttcaatc tttgtcgtac acaattgacg
    22201 tttacaaagg gctgattcgt gccgaaaaaa gctttggtca tttttgtgca tttaccgcct
    22261 tctggcccgt tatggtgaat ggtcctattg agcgtggaaa gaacttgctt cctcaaatta
    22321 actgtgaaca tgcatttgat gtttttcgac tgaaagaggg cctcttgaga atggcttggg
    22381 gcatgttcaa gaaggtggtt attgctgaca ggctagcctt ttatgtagat gatttttata
    22441 cgcattatca agttgtaagc gggtggacga tttggattgg tggtttttta tttttactgc
    22501 aagtttattg tgatttttca ggctattcag atattgccat tggctgcgcc agaatattag
    22561 gcatagatgt tatggaaaat ttcaaacgtc ctttgtattc taagaatatg tctgactttt
    22621 ggacacgctg gcatatttca ttatcgacct ggcttactga ttatgttttt tattatttag
    22681 gagcctataa agccggcagt ttgaaagttg tttttaatgt catttttgta cttacgctct
    22741 gtggtttgtg gcatggtgcc aactggccta tgatattagc atttacctta atcggtatat
    22801 ttatggcaat ccgtttcttg tggcagcaat atatagttcg ccaaataaaa cccagcaatc
    22861 tgtataagat ttcagataaa tatattcctt catttatgca tgtcatcctt acaatcagca
    22921 tttttatctt ttgctttatc ttgtttaggg tgaatagttt tgaagttacc tataacaaat
    22981 tgcatgctga agcaccttta agctggatgg aaattgctaa gccattatat cagcacatgt
    23041 ttaacttaac tagtgcaact tatttattgc catggttaaa ttttaagggt gctgttacct
    23101 taggcatcct catattttat atcctgattt tattagccag tgaatatgtc atcggtgacc
    23161 gtaagataga agaagtagta ttacctaaaa gcaatctgat acgatggagt gtgtacctgt
    23221 ttttgttatt ttccatagta tggtttggtg ttttcaataa cactaccttt gtttactttc
    23281 aatactgatc atgctaagaa aacaaattaa aaatagtatc gttttatttt ttgcgctatg
    23341 gcttgttagt cttatcatgt catttatatt tccattttat tggggagaaa gtacacaagc
    23401 tacaaagttt gaatactaca aaaaaaacaa agaaaaattc aatgctgttt atctgggtgg
    23461 cagcttagaa tacagacata tcaatcctgc aacagtagac agtatttgca agttaaacgg
    23521 actggatttt cattcattca atatggcttc tgatggtcat aatatcattc aggaaatgac
    23581 cgatttggat ggactattgg cgatagacaa tccaaaatta aaatatatat ttatcagcat
    23641 cagcagtgaa ccttattttt ttaaagcaaa tctgcatacc tcaaaatgga tttcatggca
    23701 tacagcaaaa tctacctaca gagcattgcg tataattcca actctgaagg atgacacgaa
    23761 aactaaagct cagtattgca tatattaccc gatgagctgg atggaaaatt ttttcaagtt
    23821 tggcaatatg acggatgcat taacctattt aaaaaacaaa tctcatcccg atttagctta
    23881 tcttggacca agagataacg gtttttatcc ttatgattta gaagccaatc acaccgtagt
    23941 agaagatcct aataatgata aattgatacg ggcgtctcat gatgagtttg tccgcaacaa
    24001 aaccaagcga gacagtctga caaaagaaat tttaaagagt ttttcgcaat acaaaagtga
    24061 tgataagccc aataaagctg aatataattt gctgatggaa gcttataaaa aatgcgctaa
    24121 gaaaaaaata cagctcattt ttatgcttcc gccaaaggca agaaccagtt attccttttt
    24181 attgccatta ttccatgcca tgcctgaagg gacaaagatt gaactggctg acccccgaaa
    24241 ataccccgaa ctttatcaac tggaatatgg ctataatttc catcatttaa ataataaagg
    24301 ggcaacttta gaaagcaaaa ttttgggaga acaagtagct aatttactaa aatagtcagc
    24361 aatagatatt attctgtcag acacaatttc agacggatta ttttttttat agaagcattt
    24421 atttgcttag aaagaaaaga atctttcttc atttcgtcta agatttgctg atgtaatttg
    24481 cgtgggttta caggcattaa taccatatca atacctgctt tcaaggcttt aaaatcggca
    24541 tcgggaatcg tggctacacc cagcatattc atggcatccg taaccgcaat acccttgaat
    24601 tgcagtttat ttttcaataa gccatttacg atgttagcac tgatagtaga aggttcgtta
    24661 aatgtattcc agtcctgatt attcttaaca gcaatatgcc ctaccatggt aaaaatcggt
    24721 ttggatgcct gatatactga atcaaaattt tgtatttctt tcaatgctcc gtcgatatac
    24781 accaaatttt tatgcgaatc gccggttaca tttccatgtc cgggaaaatg cttgatactc
    24841 gtagcaattt gttgctcttg cgtttgtgag ataaactgat tcgatagatg tataattgct
    24901 gaatcatttt ttccgaacga acgattatta atcacagccg tattaaatgc tttatctaca
    24961 accggagcaa aattaatttg tatgcctgtt cttctcaaag tagcgataat ggtatcggta
    25021 tattgcctca aaagtgagtc atagatcaat aagtttgttt tgggcacttt gctgctccca
    25081 aagtatttca tgttaaataa agacggttca cagtcgcagg catacaaatt ttgaagatgc
    25141 tgctgcaaag aatcaaattg atttatctga ttttttatca cgtcaagctg ccctttcaga
    25201 aaaaccacat tgctgataat ggtatcttgt aatagctttt tcgttttggt aaacgtatag
    25261 acattatccg ttgagctcac tgcatacata attagctgtg ctgccttttc tttatctgtc
    25321 aacttctcaa atgccgaatc tactagatta tctaaatcat tattttcagc ataaaaatca
    25381 tttaaagtaa aacgcgcttg tccgtttata tgtaaataca atgcacatac gcatatcaaa
    25441 ataaaaccat acctcatatt atacgtctaa aagtcttctg tgataattta tctgtccgag
    25501 atgataagag agatgcgtaa gcaaatgtaa cagcatatat tctgtcgtga ctatttttcc
    25561 atgtttttct aacgggaaat cagcctctaa ctgctcttgt gagagttgat ttaaaattgg
    25621 aacgatgacc tgtatagttg cctctatttt ctcgattaaa gttgctctcg gcacatcttt
    25681 ggaagaaaat tccaaatctc gctggcggac atatccgctg tttcctaatg tagctcctat
    25741 aaagtgattc aaattaccaa taagatgtaa gcataaattg ccaccagagt tggaaatttg
    25801 attttcaatc agccacatct ttttttcatc cttatacagc aggatttctt ctttgagttt
    25861 taataaatcg cgttcaaata attcagctaa agtttttgac agcatagtac aaaattaaaa
    25921 tccggttagt aaagcttgtt taatcttctt gtctttaggg cgtttgccaa catacatctc
    25981 gaataaggct tgtgcaaatc caatgccatt cttgagttta ccttcttgtt tctcattttt
    26041 atacaaaagc aattcgcctt ttgaagtaag ataaatatcg acaatatctt tatagccgat
    26101 cccaatttct ttcaatgtag aagttatttg ttcaacaggc tctttcagct cagcataatg
    26161 actgctaccc acgctgtctt ttatgccatt gcggatatta tcaattacca gatcagtagt
    26221 ggctaagggt gtgcaaataa tcattcta
//
LOCUS       DAOQII010000004        23480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_967_length_23480_cov_5.527556, whole genome shotgun sequence.
ACCESSION   DAOQII010000004 DAOQII010000000
VERSION     DAOQII010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23480
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_967_length_23480_cov_5.527556"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLF48_00445"
     CDS             <1..933
                     /locus_tag="PLF48_00445"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF47816.1"
                     /translation="LNLLLVCLNEINFPENEIAHEQQKAKSQLKLALEKNDYIADITF
                     RKLLFGENHPYGYASSTEAIDAIARKSLLDYYDTYVIPSLCTLYIAGKYAHDELTAIE
                     NTIGKWHKIPLSDNAILQLSEFPEIPKNPVFIKKEKSVQSSIVIGNIGIDRMNEDYPA
                     FILLNTIFGGYFGSRLMSNIREEKGLTYGIYSSLFILKHTAVFSIQTDTNVDTKDLCL
                     SEIYKEMERLQTELIPEEEIRLARNYLLGKFLHRTDGPFNQMDVYKSYCIENVNISKF
                     EEIVEGIRQQTAVSLQRIAQKYFLKEKMLEVVVG"
     gene            1051..2877
                     /locus_tag="PLF48_00450"
     CDS             1051..2877
                     /locus_tag="PLF48_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF47817.1"
                     /translation="MQHNKHILFLLANMLFSSVCLIAQTAFEPPQLTCVRNTATAPAG
                     TELNWNLPATPNPCFTGYEIYTSETGLNGTYTLYSTVSNPLQTSVVLPINKDRTAPAT
                     INYFYIINRGSCINPTTLTRTTSDTLSNQKPQPFTGITSASVINNQVHITWIPAPSIE
                     VIGYLVYNNADGYVNADTVWGRLNTTLIDTTNDPSAFAITYKIRTLEFCEDAAGLQGS
                     ITPDSAEHKTILFRLMEVDTCSQAASIQWSAYKIGSAAVINYEVQTSINGAPFSINSV
                     QSNTTSNFLLTNIPYQQSVCVRIKVNLPNGSFAFSNEQCFSANAIQKPKDDYIRNISV
                     ENGVIQIEYVYDSIAAPPQTIALYKSSNGIEFIPKTTFDSIRLRPFVLQINEPGLDVS
                     GTNYSYFLSLIDLCDSRHFSDTATTLRIAAKAKSNNKVDIGWSGFYIENSDFVQFRLE
                     KIVGTDTTLVGYFDRDEYKFTDNNLFDYTKDTVQDACFRITAEFYNLNDKTPREKLES
                     HSNVFCLTPEPQFFIPQAFRPDGYNRTIRPLILMASPDGYKFTVFDRWHEEVYTTGNI
                     TDAWDGTYKGDKAPLDGYLYYVEYTGRNGVKYTQSGMFVLIR"
     gene            complement(3243..3914)
                     /locus_tag="PLF48_00455"
     CDS             complement(3243..3914)
                     /locus_tag="PLF48_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47818.1"
                     /translation="MKRLFIVFTITALFGSLLIAGNDNKKNTPPPAPVKPAPKKEVVE
                     LANITTSTNIYTYQETPNEINLINGMKIKFAQSKRFPIPPGVNVTIPDTISERKNYEL
                     LELDVTATNTSSAVVKLDDIDLLFSGFKLFSNETPTKIYTYQYKLSFGSIYNSTQPAN
                     PELMAQYFGQTSQLMKNNYKPGQTVSSKGIIACVPKSVKKLDYIVVQVREFGLNKYYG
                     CPLQF"
     gene            complement(3993..5483)
                     /locus_tag="PLF48_00460"
     CDS             complement(3993..5483)
                     /locus_tag="PLF48_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF47819.1"
                     /translation="MSNLFVKKPLSRILSDATDSGEHQLKRTLGSSALFALGVGAIIG
                     AGIFVRTAAAAGNHAGPGVMISYIIAGIGCAFAGLCYVEFATMIPVAGSAYTYSYATL
                     GEFVAWIIGWDLILEYALGAATVAIAWSEYLNKICEAFFGFSIPYGFCHSPFQTSPIT
                     GEHGIINLPAVFILGVLTLILIRGVKESASFNSIIVIVKVIIVLVFIGLGWHFIRPEN
                     HAVIIPPNTGESGAFGWSGILRGAGIVFFAYVGFDAVSTAAQEAKNPQKGMPIGILGS
                     LAFCTVLYILFSYVLTGIAPYKDFSVTGPGHEAAVAYAISKYMHGYQWFSNLITIAII
                     AGFSSVILVMLLGQSRVFYSMSRDGLLPKQFSEIHPTYKTPAKATGLLFGLVGLFAAF
                     VPGDIVGDMTSIGTLFAFILVSIGVVIMRKNNPDAIRPFRTPLVPVVPILGVLVCGFM
                     IFGLGWQNWIRLLVWLIIGFVIYFAYSRHHSKISHTESPADHLVEK"
     gene            complement(5557..6183)
                     /locus_tag="PLF48_00465"
     CDS             complement(5557..6183)
                     /locus_tag="PLF48_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47820.1"
                     /translation="MKIIAKIVSYLFHPLLIVTWMTVLLYITNQPQFADVQQRFGKIS
                     PILPVFLYTFLFPVIAILMMKKLGFLESLEMPDSKQRIIPFIICIVLYIWTYLMMRKT
                     GYPMFLRLFMLGSISTLMLAFVINVFHKLSLHMAAITGATLTVFLWIFYSQSDLAYFF
                     IFMIILSGLVASSRLLLQAHTGREVYSGFLTGVFGQFIALMLIDKLIR"
     assembly_gap    6504..6603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6604..7634
                     /locus_tag="PLF48_00470"
     CDS             <6604..7634
                     /locus_tag="PLF48_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47821.1"
                     /translation="SIIFAPMFLKKNIFLMLLFCLGKIFAQSVNYTQWTPIAIQPYAI
                     QQLFPDTITLTEKQIYSDKGVVTLKEYSSTDKINHQFYAVYARAFSTKKEAKSILNDE
                     IKRYAIQYGVYPSEVTQKDSTGQSYTQIELLTNKQKIVMQFRELNKALYVQRVEQNLA
                     DFSNKDAQYFFMGFSISSVEKPIAQLSNTTPVIVNDNIKTETQNFIIYFPASPEYKRY
                     FIKNDSGKDYTVESYLAKDNKSGLGYQLIIRTYDKQMTQPEMWLANEVAAFTKENNIS
                     TVSENALQTANTTFSKEYVFQTKKKAYKIRYLLSGNTLYQLCISGKMKTINKKNNTAF
                     FESFVLKK"
     gene            7650..11033
                     /gene="mfd"
                     /locus_tag="PLF48_00475"
     CDS             7650..11033
                     /gene="mfd"
                     /locus_tag="PLF48_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNF47822.1"
                     /translation="MDINQLLFLFRDDERVEKLKQQLEQPQSRVLLGGVLGSARSFIA
                     SSLFLTTEFTQVFVCNDADSAQYLQNDLQNLLDKKEILFLPSSYKKSFTFAEQSNNNI
                     LLRAQTLNALLNAKKGGELLVTYPEALQELLVRKETLQKNTLHLKTGEKADIDFLLDV
                     LLEYGFERTDFVYEPGEFSIRGGIIDVFSFGNELPYRIELFDDEVESMRTFNPESQLS
                     ERKISELTIIPNIQNHFVQELQSSLFEFLPENTIFWMEDMPLFKELIEKQEQRALEEY
                     EQLAKSKTKEHPFLNKQFSQLFLNSNDLVLQLSHYRIITQPPLIPLLLKDENGSSLLQ
                     VDIRPQPSFGRNFDLLIQDIQALQKEKYQVFLCSENARQLERFHHIFQDKQAQISFHP
                     IYQAFTSGFIDKDLKIALYTDHQIFERYHKYKTKSGFNRNKALSIRHLKDLNPGDYVT
                     HIDHGVGVFSGLQKLTINGKEQEMVRLIYKDEDVLYVNINSLHKIAKYTGKEGHAPKI
                     NKLGSDVWTNLKNKTKKKVKDIAAELIKLYAMRKVQPGFAFNKDTYLQDELEASFMYE
                     DTPDQYKATLDVKADMEKPHPMDRLICGDVGFGKTEVAIRAAFKAVTDGKQVAVLVPT
                     TILAWQHYKTFSSRLNEFGVTVDYLNRFKSAKEKKETLQKAKDKKVDIVIGTHAILSK
                     DLEFKDLGLLIIDEEQKFGVSAKEKLRQLALNIDTLTLTATPIPRTLKFSLMGARDLS
                     NIMTPPTNRIPVSTEVHAFDIKFIKEAIEFEVYRGGQVYFVHNRVKDIHEIEILLKKM
                     MPDISVATAHGQLEGDKLEEIMLGFINGEYDVLLSTNIIESGLDIPNANTILIHNAHQ
                     FGLSDLHQLRGRVGRSNKKAFCYLISPPKSVLTPEAKKRLQTLEEFSDLGSGFQISLR
                     DMDIRGAGNLLGAEQSGFIADIGFEMYHKILDEAIQELKYTDFKEVFKEKIEEQKQFV
                     YECTIDTDVEMLIPQEYVQNTEERLRLYTELDEITNEEKLNVFAKKLTDRFGALPKQV
                     NELFDGLRIRWVAKKIGFERIILKNNKLRCYFLENQKSPYYDSPYFNLVMTHIQNAKK
                     KCVIKQSGNSLILIYDNIKTMHETEAIFKDIDEKVFKEN"
     gene            complement(11144..11806)
                     /locus_tag="PLF48_00480"
     CDS             complement(11144..11806)
                     /locus_tag="PLF48_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47823.1"
                     /translation="MRMRNYLVLLILLLFTKGIDAQLLIDAGSNNSDIIESSSFHFNG
                     LNGWPTADYSGNSIYANLYEDEYYLIGVKDAPLGGTVSKNIYVNNNRNYTISTLIKTV
                     YVAANQTFFAGLLLSDGTNRITFDITWGEYSISQIYNGKGSDIQTWKLCEFIEGDVSR
                     PERLYNVLQIEKKNNNLNFYINSHFVQQIKTPFWFKLSQVGFNVSNNTEVAVDYINVL
                     YR"
     gene            complement(11823..12065)
                     /locus_tag="PLF48_00485"
     CDS             complement(11823..12065)
                     /locus_tag="PLF48_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47824.1"
                     /translation="MISKFLLPILITIVLLSSCAKNKLCDCTYVTTGMPAGVYPLEGT
                     QFLTHKMVNKKLCSSIEDDLNKNVTDADQRVGCELH"
     gene            12204..12572
                     /locus_tag="PLF48_00490"
     CDS             12204..12572
                     /locus_tag="PLF48_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF47825.1"
                     /translation="MEIGTKIRKLRELKGLSQENMAYELDMSIAGYGKIERNEVSVNY
                     DKLLKISETLNVRIEDIIGFDEKVAFNNFNSKIQQQIGRYTYADEIKLLYEDKIKLLE
                     DKIILLEELNRTLKLKIENK"
     gene            complement(12580..12837)
                     /locus_tag="PLF48_00495"
     CDS             complement(12580..12837)
                     /locus_tag="PLF48_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47826.1"
                     /translation="MEITGYGLTESEAKRSFEITLQEFLRYTIKKNTLRNELERLGWT
                     YKGKKLLQPDFSDLLLKNKDLHHIIDKKEFKKFDEALQIPA"
     gene            complement(12990..15002)
                     /gene="uvrB"
                     /locus_tag="PLF48_00500"
     CDS             complement(12990..15002)
                     /gene="uvrB"
                     /locus_tag="PLF48_00500"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNF47827.1"
                     /translation="MEFKIHSKFNPAGDQPEAIRLLSLGIDKGETSQVLLGVTGSGKT
                     FTIANVVEKMQRPTLVLSHNKTLVAQLYSEFKEFFPDNKIEFFTSYYDYYQPEAYIPT
                     TNTYIEKDLSINAEIEKLRLSTTSSLMSGRRDVLVVASVSCIYGLGNPAEFKNQIIRI
                     SKGQTISRNTFLFQLVSILYARTSADFQRGNFRVIGDTVEVFLAYADYAYRITFWGDD
                     VEELESFNPATGKVIEQMKDIAIFPANIYIAPQNMLPQIIHEIQDELFDQIKFYNEMG
                     KYEEAKRLDERVNFDLEMIRELGYCSGIENYSRFFDRRHPGDRPFCLLDYFPDDFLCV
                     IDESHVTVPQVGGMYGGDRSRKQNLVEYGFRLPSALDNRPLNFSEFESLLNQTIYVSA
                     TPGDYELMKTEGEFVEQVVRPTGLLDPPIDVRPSVNQIDDLLNEIQERIKVNERVLVT
                     TLTKRMSEELTKYLTRLNIKCKYLHSDIDTMERVEIIQGLRLGEFDVLIGVNLLREGL
                     DIPEVSLVAILDADKEGFLRNVRSLTQTAGRAARNANGKVIMYADRITGSMKKTIDET
                     DRRREKQIAYNIEHHITPKTIYKSKEEIANKSTILDIRRNVQDKEEEVIETMVAAEPM
                     GEYGTAEALDKQIEKVKKAMQKASKQMDFMEAARLRDELFRLQGLL"
     gene            15096..16382
                     /locus_tag="PLF48_00505"
     CDS             15096..16382
                     /locus_tag="PLF48_00505"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF47828.1"
                     /translation="MRKIITLLLLMQIVQFLQAEDCTNGRYIRQVFSRVQVHKNIVYA
                     RKIQSNGAPIYLRYDVYEPYGDTLSERPVMLLIHGGAYLKLLDQNSPDIVLMCDYFAK
                     RGYVTVSIDYRQESNFLSILSAENMTKAVARALIDTRDAVDHLMRTYTNGNPYRIDTS
                     RAFIGGVSAGAVSTLFISYLDSLQMLPTQMQQWIIEATGPESDSILRHRFDLVKPKAA
                     ISVSGALLDTGWLVNNNIDLLLIHGSLDNIVPYNYDYPLGVPMLPKLFGGKALYPVAQ
                     RRGIRTEFEDWIGYGHVPFMNLDLGSIITLHLISPDRLDSTERHIAQFLYRHLGCNLV
                     SGVRTNLHAEKLNIYPNPATSYFNLPLPKDETINKWQLEIYSLDGKLQLAKEVNSNVG
                     NIRIDELHQSGTYIIKLSVVKNNEEHVYMGEVQVLK"
     gene            complement(16387..17289)
                     /locus_tag="PLF48_00510"
     CDS             complement(16387..17289)
                     /locus_tag="PLF48_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNF47829.1"
                     /translation="MKKKIYIAGCGGMLGEAFYKIFKDDYELKCTDKDVNDTWLSFLD
                     FRDFEAYKKDVFDFKPDYLFHLGAHTSLEYCDTNIEDAYLTNTTSVENAVHISNTLNI
                     PMLYISTAGIFDGSKDTFDDWDIPNPLGHYARSKWAGEVFVQQNKTQHLICRAGWMMG
                     AGPKKDKKFIQKLMQQLKDGKKELFIVNDKLGTPTLTYDFARNVKVLLQNEVWGLYNM
                     VCGGTTGRLEVATELVRLLDKENEIKITAVDSDFWAKEYFSQRPPSERLINMKLNLRQ
                     LNVMRDWKVCLKEYVDEYYQDYLD"
     gene            complement(17290..18387)
                     /locus_tag="PLF48_00515"
     CDS             complement(17290..18387)
                     /locus_tag="PLF48_00515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF47830.1"
                     /translation="MSRELNKKHKVLFLSPYPFGKAASQRLKYEQYFDYFDEHGFHPV
                     TSSFVDDSFWEVIYKPGFIFQKITGTIRGYVRRFLDLLRLRKYDIVYVHLWVTPLGPP
                     MFEWLVRKLSKKIVYDIDDMIYKSEGSAMNQLIYMLKGKDKSIAMMKYADHVITCTPD
                     LNDFALQYCAHCTDISSTLDTLRMFPVNTYTNDKPVVIGWTGTHSTVPYLYLLTEVFQ
                     QLAKERAFKLRVIGNFNFHMEGVDYEYLDWNKEDEAKQLQGMDIGVYPLPTDAWVMGK
                     SGLKALTYMTFALPVVATNVGAAINRVMTDNVDGYLVKTNEEWLEKLRYLIDYPEERR
                     RLGEHARQTVLDKFSIEANKPVYLGVLKSLV"
     gene            complement(18384..19040)
                     /locus_tag="PLF48_00520"
     CDS             complement(18384..19040)
                     /locus_tag="PLF48_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF47831.1"
                     /translation="MNYKRIYEYRFKDVDKERKLLVWKEIAEFLYTKHLNAPEKIIDP
                     AGGLCEFINAVPSSEKWTVDLDEGVKLHADKNVKTIIGNNLNVELPDNYFDGIFISNF
                     LEHLANQSEVAAFLQRMYNILKKGGRIVIMGPNFRHTYKDYFNFADHTVILTELGVAE
                     HLYGAGFEIVKVYPKFLPLSFRGNRLLPLNSFIAKMYFKFPVFWKILGQQFLIIAEKS
                     "
     gene            19118..20212
                     /locus_tag="PLF48_00525"
     CDS             19118..20212
                     /locus_tag="PLF48_00525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF47832.1"
                     /translation="MKKIRQADLSTDYLQVAQDVNTKLTELLTSGTFIGGQEVSAFER
                     ALSEYLGVKRVISCGNGTDALQLALMSLNLPRGSKIIVPAFTYIAPIEVIKLLGFEPV
                     YADVDVRTFNICLSTIADVYTEEVKAIVVVHLFGQLISDIDEIAKFCDQKQITLIEDN
                     AQSLGAEKKLNRNSICTTSFFPTKNLGCYGDGGAIYTNDEAKANTIRMMANHGQSKKY
                     YHDIIGINSRLDALQAGILNVKLPGIDQLIQKKKNISYIYDECFKNIPFLTIPIQESI
                     HTFHQYTLIVDARYRDELQNYLADHHIESTVYYPLPAYKQKAYLKDVFLPVCENLCQT
                     VLSIPIHLYLSESEISYICETIVEFYKNKS"
     gene            20214..20786
                     /locus_tag="PLF48_00530"
     CDS             20214..20786
                     /locus_tag="PLF48_00530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF47833.1"
                     /translation="MSYFKHESAYIDDGASIGKDTKIWHFCHIMQAQIGEGCVLGQNV
                     FIANKVIIGNRVKIQNNVSVYEGVDLEDDVFIGPSVVFTNVINPRSFIERKEEYKYTL
                     VKKGATIGANVTVVCGITIGEYAFVGAGSVLTKDVPAYALMVGNPAKQTGWMSEAGGK
                     LIFGFGQKATCPVNGELYRLQEGVVIKVTE"
     gene            complement(20839..21402)
                     /gene="efp"
                     /locus_tag="PLF48_00535"
     CDS             complement(20839..21402)
                     /gene="efp"
                     /locus_tag="PLF48_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNF47834.1"
                     /translation="MASTSDIRNGMCINFNSDIYQVVEFLHVKPGKGAAFVRTKLKSV
                     TSGRVLENTFPSGAKLDEVRVEHREYQFLYKEGDQYMFMNNETYEQVAIPEAIINAPE
                     FLEEGMIVKIAVRAEDELPLTCTLPATVVVEVIYAEPGIKGDTATNTLRPCTVSTGAK
                     VMIPLFVNIGDKIKVNTDTGDYMERVK"
     gene            complement(21468..21656)
                     /gene="rpmF"
                     /locus_tag="PLF48_00540"
     CDS             complement(21468..21656)
                     /gene="rpmF"
                     /locus_tag="PLF48_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNF47835.1"
                     /translation="MAHPKRRHSSTRRDKRRTHYKMEAPNVSTCKKTGENHLSHRAYI
                     HGNDLYYNGKVLIEGYVK"
     gene            complement(21663..22205)
                     /locus_tag="PLF48_00545"
     CDS             complement(21663..22205)
                     /locus_tag="PLF48_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNF47836.1"
                     /translation="MGDVLNEIRIPHAGLRKEIHEFTFGINEKFFTDFEKSQIQKCNI
                     DVHINMDKRQEPILLEVDLEGEVWSECDRCTAEIPLAVHTSFVIYVKYALTEEMKKAE
                     DIDILYIGKDDQDIDITALVYDYLHLTLPIHRICDKPGKTEYCDKEIVSLLEKKQHEN
                     DTPSDDPRWAELNKLKDKLN"
     gene            complement(22388..>23480)
                     /locus_tag="PLF48_00550"
     CDS             complement(22388..>23480)
                     /locus_tag="PLF48_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF47837.1"
                     /translation="TNYVQSFLGNLGGSIQNNAVGKLMTLAYAPTGMGTNGTNILKSM
                     YDGNISISDGPLIVQGFSTDGNNTVNEKFMGDDFIVQTTDTNKLFLNLQYVSSAEYGT
                     ITSIDLILGTSSGETTVSYPVTGTSASLSLSKIISLFGAANVPMDQYFYIREALSTTK
                     NYGAGSPYILTNETFHALTNPVWIKIKPVSGLPVELLTFDLAEENNMVRLNWKTASEI
                     NNKGFEIQRSTDNIHWENIGWADGHATSTIEHSYTFLDEQPFLGENYYRLKQVDFDST
                     FEYSKILFIYFKSSKELVLYPNPATHILYIHAQQEFKTVNIYNSTGAKVAQRPVNNRM
                     VNLADLASGIYFIGFDDEKDGHRVKIFKN"
BASE COUNT         7554 a         4342 c         4228 g         7256 t
ORIGIN      
        1 ttaaacttgc tgttagtttg tctaaatgaa ataaattttc ctgaaaatga aattgctcac
       61 gagcaacaaa aagccaaatc ccaattaaaa ctagcgttgg aaaaaaacga ctatatcgcc
      121 gatattacat tccggaagtt gttgtttggc gaaaatcatc cttatggtta tgcctcatct
      181 acagaggcca ttgatgccat tgcccgaaaa tcacttttag attattacga cacatacgtg
      241 ataccatcgc tttgcacgct ttatattgcc ggaaaatatg cccatgatga attgacggct
      301 attgaaaata ccatcggaaa atggcataaa atacccttat cggataatgc aattttacag
      361 ctctctgaat ttcctgaaat acccaaaaat cctgttttta taaaaaaaga aaagtcggtg
      421 caatcttcta tcgtcatcgg caatatcggt atcgaccgca tgaatgaaga ctatccggcc
      481 tttatcttgt tgaataccat atttggcggt tatttcggct caaggctgat gagtaatatc
      541 cgcgaggaaa aaggcttgac ttatggcata tattccagct tatttatcct gaaacatact
      601 gcagtatttt ctattcaaac cgataccaat gttgatacta aagatttatg tttgagtgaa
      661 atatacaaag aaatggagcg tttacaaacg gagttgatac cagaagaaga aatcagactg
      721 gcacgcaatt atttgttggg caaatttttg cacagaacag acggaccgtt taaccagatg
      781 gatgtatata agtcatactg tatagaaaat gttaacattt ctaaatttga ggaaattgta
      841 gagggaatac gccaacaaac ggcagtatct ttgcaacgta tagcccaaaa atactttctt
      901 aaagaaaaga tgctggaagt agtagtgggt taatgatgaa ttattaatga taaattttga
      961 atgttaaatg attaatgaaa aatagagtac aatcccattc ccctcttaag tggggacata
     1021 ggggtgtgtc ttccgctttt aactcaacga atgcaacaca ataaacatat tctttttcta
     1081 ctggcaaata tgctatttag ttctgtttgt ttaattgcgc aaactgcatt tgaaccaccg
     1141 caattaactt gtgtacgcaa tacggcaaca gcaccagcag gcacagagct caactggaat
     1201 ttacctgcaa cacccaaccc ctgttttacc ggctatgaga tttatacttc agaaacggga
     1261 ctcaacggca cttatacgct ttattctacg gtaagcaatc ctttacaaac ttccgtggtt
     1321 ttgccaatca ataaagaccg tacagctccg gcaactatta actattttta catcattaac
     1381 cgaggttctt gtatcaaccc gacgacttta acaagaacaa catctgatac gctaagtaat
     1441 caaaagccac aaccttttac cggtattacg tctgcaagtg ttatcaataa tcaagtacat
     1501 attacatgga tacctgcacc cagcatagag gtaataggct atttggtgta taataatgct
     1561 gacggctatg taaatgcgga tacagtttgg ggcagactta atactacttt aatcgatact
     1621 acaaacgatc caagtgcttt tgcgataaca tataaaattc gcacgctgga attttgtgaa
     1681 gatgctgccg gcttgcaagg gtctataact ccagactctg ctgaacataa aactatctta
     1741 tttagattaa tggaagtaga tacttgttca caagcggcat ctatacaatg gtcggcatat
     1801 aaaatcggtt cagctgctgt gataaattat gaagtacaaa cgagcataaa tggcgcacct
     1861 ttttccatca actctgtaca aagtaatacg acatccaatt tcttactaac caatatccct
     1921 tatcagcaat ctgtctgtgt gcgcataaaa gtgaatttac caaatggttc tttcgcattt
     1981 tctaacgaac aatgcttttc tgcaaatgcc attcaaaagc caaaagacga ttacatccga
     2041 aatataagtg tagaaaacgg cgttattcaa attgaatatg tgtatgattc tattgctgca
     2101 cctccgcaaa ctatcgcact atacaaaagc tcaaacggca ttgagtttat tcccaaaaca
     2161 acatttgact ctattcgttt acgaccattc gttttacaaa tcaatgaacc gggcttggat
     2221 gtatcaggta caaactattc ttactttctt tcattaattg atttgtgtga cagccgtcac
     2281 ttctcagata ctgccactac cttgcgcatt gcggcaaagg ctaaaagcaa taacaaagtg
     2341 gatataggat ggagtggatt ttatatcgaa aattctgatt ttgttcaatt ccgtttagaa
     2401 aaaattgtgg gtacagatac tactttagtg ggctatttcg acagagatga atacaaattc
     2461 accgacaaca atttattcga ttatacgaaa gacactgttc aggatgcttg tttccgcatc
     2521 accgctgaat tttataacct gaatgacaaa acacctcgcg aaaaattaga atcgcattct
     2581 aatgtttttt gtttaactcc cgaaccacag ttttttattc ctcaagcttt ccgtccggat
     2641 ggttataacc gcacaatccg tccgctgatc ttgatggcat caccggatgg ttataaattt
     2701 accgtgttcg accgctggca tgaagaagta tatactaccg gcaatattac agatgcctgg
     2761 gatggcactt ataaaggtga taaagcccca ttagacggct atttatacta tgtggaatat
     2821 acgggaagaa atggcgtaaa atatacccaa tctggcatgt ttgtgctgat aagataactg
     2881 tacttatatt tcaattttgg gttctactcg tatggaactc agcaaacacg actatttatt
     2941 tctttcgatt atactgaagc tcctagcaaa tataaaatca ggtataatag acagaatagt
     3001 gccaatttat tttaagatta taccgataga tttccagctt taattaacct tgtaaatcat
     3061 ataaagggaa gtaggctatg cggaagttgt ggtatttagc cacctttttt ttacctgagt
     3121 tagggttaag aaaaataagc ggataaaata gtagatttct catttttttc ttccacttta
     3181 acaaagtgaa ggaattaaat gctgtaagaa accaactatc tttaaattga accatttggt
     3241 gattaaaatt gtaagggaca tccgtaatat ttgttcaatc caaattctct gacttgcacc
     3301 acgatatagt ctaatttttt cactgatttt ggtacacacg caataattcc ttttgaactt
     3361 accgtttggc cgggcttgta attgtttttc attaattgag atgtttgtcc gaaatattgt
     3421 gccatcaatt ccggatttgc aggctgtgtg ctgttgtata tcgaaccgaa cgataatttg
     3481 tactggtaag tataaatttt agttggagtt tcgttggaaa agagtttaaa tcctgagaac
     3541 aacaaatcaa tgtcgtctaa ttttaccaca gcagagctcg tattggtagc tgtaacatcc
     3601 agctctagta attcataatt cttgcgctcg ctgattgtat ccggaatagt aacatttact
     3661 cccggtggaa taggaaaacg tttggattga gcaaatttaa ttttcatgcc attgattaaa
     3721 ttaatctcat tgggcgtttc ttgatacgta taaatattag tagatgttgt gatgtttgcc
     3781 aactctacca cttctttttt aggagcaggt ttcaccggag caggcggagg ggtatttttc
     3841 ttattatcat ttccggcaat aagcaaagaa ccaaataatg ccgtaatagt aaatacaata
     3901 aaaagacgtt tcataaagca atttatagtg taataataaa gatacgcact tttctaacaa
     3961 aaagtgtgcc ttttttaagg ctaaataaaa aactacttct ccaccaaatg atctgcaggc
     4021 gattctgtat gactgatttt gctgtgatga cggctatagg cgaaataaat aacaaagccg
     4081 ataattaacc atactaaaag ccgaatccaa ttttgccaac ccaacccgaa tatcatgaat
     4141 ccgcaaacta acacacctaa gatgggaact acaggaacga gtggggttct gaatggacgt
     4201 atagcatccg gattgttttt acgcataata actactccta tcgatactaa aatgaatgca
     4261 aataaagtac ctatactggt catatcacct acgatatcac ccggtacaaa agcagcaaat
     4321 aagcctacta aaccaaaaag caatcctgta gcttttgccg gtgttttata agttggatga
     4381 atttctgaga attgttttgg caataaacca tctctgctca tagaatagaa tacacgagat
     4441 tgtcctaaaa gcataaccaa tattacagat gaaaatccgg caataatagc tatggttatc
     4501 aaattgctga accactgata gccatgcata tatttggaaa ttgcataagc aactgcagct
     4561 tcatgtcccg gacctgtaac actaaaatct ttatatggag caatgcctgt aagtacataa
     4621 gagaataaaa tatacaatac cgtacaaaat gccaaagaac ctaaaatgcc aattggcatt
     4681 cctttttgag gattcttggc ttcctgggct gctgttgaaa ctgcatcaaa cccaacataa
     4741 gcaaagaata ctattcctgc accacgcaat attccactcc atccaaatgc tccggattct
     4801 ccagtatttg gtggtataat tactgcatga ttttctgggc gaataaaatg ccagcctaat
     4861 ccgataaaca ccaatacaat tatcactttt acaattacaa taatagaatt aaatgaagcc
     4921 gattctttta caccacgaat taatattaat gtcaatacgc ctaaaataaa aacagccggc
     4981 aagttaatga tgccatgttc tcctgtaata ggcgacgttt gaaatgggga atggcaaaac
     5041 ccgtaaggga tactaaatcc aaaaaatgct tcacaaattt tatttaaata ttcagaccat
     5101 gcgattgcaa ctgttgcagc acctaaagca tattctaaaa ttaaatccca tcctattatc
     5161 catgcaacaa actctcccaa tgtagcatat gagtaggtgt aagcacttcc ggccacagga
     5221 atcattgtgg caaattcaac ataacataat cctgcaaatg cacagcctat tccggctata
     5281 atataagaaa tcattactcc gggtccggcg tggtttcctg cagcagcggc agtacgaaca
     5341 aatatacctg ctccgattat tgcaccaact ccgagtgcaa ataatgctga tgagcctaat
     5401 gtgcgcttca actgatgttc acctgagtca gtagcatcac tcaagattct gctcaaaggt
     5461 ttttttacaa aaagattact catattcatt tggttagagc ggaaagataa gtaattttta
     5521 aaaataaatt acctaagcgt taccaagttt ttggtattag cgtattaatt tgtcaattaa
     5581 cataagtgcg atgaattgcc caaacactcc ggtaagaaat ccggaataaa cttctctgcc
     5641 cgtatgggct tgcaacaaca gcctcgagga ggcaaccaga ccactcagaa ttatcataaa
     5701 aatgaaaaaa tatgctaagt cgctctggga gtaaaatatc cataaaaata ccgtaagtgt
     5761 tgcacccgtt atagccgcca tgtgcaagct gagcttatgg aaaacattga taacaaacgc
     5821 cagcattaat gtagagattg aacccagcat aaataaacgc agaaacatag ggtatcccgt
     5881 tttgcgcatc atcagatagg tccagatata gagtacaata cagatgataa aaggaataat
     5941 acgttgtttg ctgtcgggca tttccagact ttccagaaat ccgagttttt tcatcatcaa
     6001 aatggcaatt accggaaaaa gaaaagtata taaaaacaca ggcaatatgg gagatatttt
     6061 tccgaagcgc tgttgcacat cagcaaattg aggctggttg gttatgtata acaagaccgt
     6121 catccatgtt actattaata acggatgaaa cagataagaa acgatttttg caattatttt
     6181 catcctttca aagatagggt atttaaactt gttttggata aatcaaattg ttatcctcat
     6241 acaaagtgaa tcaaattggg tatatttata acacttactg tttttcctga ttgcaggata
     6301 aaaaacagaa aataaaataa tcatgtagga tatttttgat tttagtaaat ttgatgtgaa
     6361 atgcagacgt taactattga attattaaat gatgatgcaa taaacttatt gcaagattta
     6421 gaattactga agctgattcg catatcaaac cataaatcgg aaatacccga ctggcataaa
     6481 tccattttag aggaaagaat tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnntttccat tatctttgcg ccaatgtttt tgaagaaaaa catcttcttg atgttgttgt
     6661 tttgtttggg aaaaattttt gcccaatcgg taaactatac tcaatggaca cctatcgcca
     6721 tccagcctta tgctatacaa caactttttc cagatacaat tactttgacg gaaaaacaaa
     6781 tttattcgga taaaggtgtt gtaacgctta aagaatacag ttcaacagat aaaataaatc
     6841 atcagtttta tgcggtttat gcccgtgctt tttcgactaa aaaagaggca aaatctattc
     6901 tgaatgatga aataaaacga tatgctattc agtatggtgt atatccaagc gaagtaacac
     6961 aaaaagacag tacaggacaa tcctataccc aaattgaact cttgacgaac aagcaaaaga
     7021 tagtcatgca attcagagag ctaaacaagg cattatatgt acagcgtgtc gagcaaaatt
     7081 tggctgattt ttcaaataaa gatgctcaat atttctttat gggatttagt atttcttctg
     7141 tagaaaaacc aatagcacaa ctaagcaata ctactcctgt tattgtaaat gacaacatta
     7201 aaacggaaac tcagaatttt attatctatt ttccggcatc accggaatat aaacgctact
     7261 ttataaagaa tgattcaggc aaagattata cagtagaaag ctatttggct aaagacaata
     7321 aaagtgggtt gggctatcag ttaattattc gtacttatga taaacaaatg acacaaccgg
     7381 aaatgtggct ggcaaatgaa gttgcagctt ttaccaaaga aaacaatata agcaccgttt
     7441 cagaaaacgc actgcaaacg gcaaacacca cttttagcaa agaatatgtt ttccagacta
     7501 aaaaaaaagc ctacaaaata cgctatttgt tatccgggaa taccttgtat cagctttgta
     7561 tttccggaaa aatgaagaca atcaacaaaa aaaacaatac cgcttttttt gaaagttttg
     7621 ttttgaaaaa gtaatacgtt aaataaagaa tggatattaa tcaattgctt tttttattcc
     7681 gcgatgatga gcgtgtagaa aagctgaaac aacaacttga acaaccgcaa tcccgagtat
     7741 tgctcggtgg cgtattgggg tctgcacgaa gttttatcgc gtcttcgctt tttctcacta
     7801 cagaatttac gcaggttttt gtttgtaatg atgccgactc cgcgcaatac ttgcaaaatg
     7861 atttacaaaa tcttttggat aaaaaagaga tattatttct tccctcttcg tacaaaaaat
     7921 cattcacctt cgcagagcaa agcaacaata atattttatt gcgggcacaa acactgaatg
     7981 ccttgctgaa tgccaaaaaa ggtggcgagt tgttagttac ctatcccgaa gccttgcagg
     8041 aattattagt gcgcaaagaa acgctgcaaa aaaatacgct tcatcttaaa acaggtgaaa
     8101 aagcggatat cgattttttg ctggatgtgc tcttggaata cggttttgaa cgcaccgatt
     8161 ttgtctatga gcccggtgag ttttctatcc gtgggggcat tatagacgtg ttttcatttg
     8221 gcaatgagct gccctaccgc atcgagttgt ttgatgatga agtggaaagt atgcgtacct
     8281 ttaatccgga gtcgcaatta tcagaacgca aaatttcaga actcactatc atcccaaata
     8341 ttcagaatca ttttgtacag gaattgcaat cttctttgtt cgagtttttg cctgaaaaca
     8401 ccatcttttg gatggaagac atgccgctct tcaaagaact catcgaaaag caagagcaaa
     8461 gagccttgga agaatatgag caattagcta aaagcaaaac caaagaacat ccgtttttaa
     8521 acaaacaatt cagccagctg tttttaaaca gcaatgactt agtattacag ctatcccatt
     8581 atagaattat aacacaacca ccccttattc ccctcctttt aaaggatgag aatggaagca
     8641 gtctgctgca agttgacatt cgccctcagc cttccttcgg tagaaatttt gatctgctca
     8701 ttcaggatat tcaggctttg caaaaagaaa aataccaggt gtttttatgt tctgaaaatg
     8761 ccagacaatt agaacgcttt catcatatct ttcaggataa acaagctcaa atttcttttc
     8821 atcccatcta tcaggcattc acctccggat ttatcgacaa agatttgaaa attgcccttt
     8881 ataccgacca ccaaattttt gagcgttatc ataaatacaa aaccaaatcc ggatttaacc
     8941 gcaataaagc cttatcaata cggcatctta aagaccttaa tcccggagat tatgtaacac
     9001 atatcgacca tggtgtaggc gtattttcgg gtttgcagaa actcaccatc aacggcaaag
     9061 aacaggaaat ggtgcgcttg atttataagg atgaagatgt gttgtatgtc aacatcaact
     9121 cactgcacaa aatagcaaaa tacacgggca aagaagggca tgcacccaaa atcaataaat
     9181 taggcagtga tgtttggacg aatcttaaaa acaagactaa aaagaaagtt aaagatattg
     9241 ccgctgagct gataaagctc tatgccatgc gcaaagttca gcccggcttt gcttttaata
     9301 aagacaccta tttacaagat gagctggagg ccagttttat gtatgaagat acgcctgacc
     9361 aatacaaggc tactttagat gtaaaagccg atatggaaaa accgcaccca atggacaggt
     9421 tgatatgcgg cgatgtaggt tttggaaaaa cggaagtagc gatacgtgcg gcatttaaag
     9481 cggtaacgga tggcaagcaa gtggcagtac tggtgccgac taccatcttg gcatggcagc
     9541 attacaaaac tttttcatcc aggctgaacg agtttggtgt aacagtggat tatctaaaca
     9601 ggtttaaatc agctaaggaa aaaaaggaaa cgctgcaaaa agccaaggat aaaaaagtag
     9661 atattgtcat tggtacgcat gccattttaa gcaaagattt agagtttaaa gacttgggtt
     9721 tattaatcat agatgaagag caaaaattcg gtgtttcagc aaaagaaaaa ttgcgtcagc
     9781 tagcattaaa tattgatacg cttacgctaa cagcaacacc gataccgcgt accctgaaat
     9841 tttcgttgat gggtgcacgc gatttatcaa acatcatgac accacctaca aaccgaattc
     9901 cggtaagtac ggaggtgcat gctttcgata taaaatttat taaagaagcg atagagtttg
     9961 aagtgtatcg tggaggacaa gtttattttg ttcacaaccg agtgaaagat atacatgaaa
    10021 tagaaatatt gctcaaaaaa atgatgcctg atatttctgt ggctaccgca catgggcaac
    10081 tggaaggcga caagttggaa gaaataatgc tgggatttat caatggagag tatgatgttt
    10141 tgctgagtac caatatcatt gaaagtggtt tggatattcc gaatgccaat actattctca
    10201 ttcataatgc acatcagttc ggcttgagcg atttgcatca gttgagagga agagtggggc
    10261 gttccaacaa aaaagcattt tgttatctga ttagtccgcc gaaaagtgtg ttgacacccg
    10321 aagccaaaaa acgcttgcaa acactggaag aattttcgga tttgggatcc ggtttccaaa
    10381 tatcattgcg cgatatggat atccggggtg cgggcaattt attaggcgca gaacaaagcg
    10441 gatttattgc agatatcggg tttgaaatgt atcataaaat attggatgaa gccatacagg
    10501 aattaaaata caccgacttc aaagaagtat ttaaggaaaa gatagaagaa caaaaacaat
    10561 ttgtatatga atgcacgata gataccgatg tggagatgct gataccgcag gaatatgtgc
    10621 aaaatacaga agaacgactg cgattatata ctgagctgga tgagataacc aatgaagaaa
    10681 aattaaacgt atttgcaaaa aagctgaccg accgttttgg tgctttaccc aagcaggtaa
    10741 atgaattgtt tgatggtttg cgaatacgtt gggttgccaa gaagattgga tttgagcgca
    10801 ttattttgaa gaataataaa ctacgttgtt atttcttaga aaatcaaaag tcgccatact
    10861 atgatagtcc gtattttaat ttggtgatga cacatataca aaacgctaag aagaaatgcg
    10921 tgattaaaca aagcggtaac agcttgattt taatttacga caacatcaaa accatgcacg
    10981 aaacagaagc aatttttaaa gacatagacg aaaaagtgtt taaagaaaat tagcatatag
    11041 tgtgccacga gcatcgtgat acacctaatt taaatttagt tatcgtttgg tgtggacacc
    11101 aaacgaaaga acgaaagctg aagaataaaa acaaaaatta atgttatctg tataatacat
    11161 ttatataatc tactgcgact tctgtattgt tggaaacatt aaaaccaact tgacttaatt
    11221 taaaccaaaa aggagtctta atttgttgta caaaatggct atttatatag aaatttaaat
    11281 tgttattctt tttttctatc tgaagcacat tgtataacct ttctggtcta ctaacatcac
    11341 cttcaataaa ttcacacaac ttccaagtct gtatatcaga tccttttcca ttgtatatct
    11401 gactgatcga atattcaccc catgttatat caaaagtaat tctatttgtt ccatcgctta
    11461 aaagcaatcc agcaaagaat gtttgatttg ctgctacata aactgttttt atcaatgtag
    11521 atattgtgta attcctatta ttgtttacat atatattctt acttaccgtc cctccaagag
    11581 gtgcatcttt taccccaatt aagtaatatt cgtcttcata aagattcgca tatatactat
    11641 ttcccgaata gtcagctgtt ggccaaccat ttagtccatt aaaatgaaaa cttgagcttt
    11701 ctataatatc agagttatta gaacctgcat ctattaacaa ttgagcatct attccttttg
    11761 tgaaaagtaa tagaattagt aatactaagt aattacgcat tctcatgtct ttgatttaat
    11821 tgttaatgta actcacaacc gacgcgctga tctgcatcag tcacattttt attcaaatca
    11881 tcttcaatgg aactacatag ttttttattg accattttat gtgtaagaaa ttgagttcct
    11941 tctaatggat aaactccagc aggcatgcct gttgtaacat aagtacaatc acaaagttta
    12001 ttttttgcac aagaacttaa caaaacaata gtgattaaaa ttggaagtaa gaattttgat
    12061 atcataaaat ttaatttaat tatttgtagc aaaagtatac tttcttaata ttctaatttg
    12121 tttctatggg tagtaaattg taacttctga ttaagaaaat tgtacttata gtaaatattt
    12181 acttaatttt gtatttttat gatatggaaa ttggtacaaa aattagaaaa ttaagagagc
    12241 taaagggtct aagtcaagag aatatggctt acgaattaga catgtccatt gctggatatg
    12301 gtaaaattga aagaaatgag gtgagtgtaa attacgataa attattaaaa atatcagaaa
    12361 ccctaaatgt acgtatagaa gatattatag gatttgatga aaaggttgcg tttaataatt
    12421 tcaatagtaa gatacaacaa caaattggca gatacactta tgccgatgaa ataaaattgc
    12481 tatatgaaga caaaattaag ttactcgaag ataaaataat tctacttgaa gaactaaata
    12541 gaactttgaa actgaaaatt gaaaataagt aaggtgaaat tacgctggta tttgcaaggc
    12601 ttcatcaaac ttcttaaatt ctttcttatc aatgatgtga tgcaaatctt tatttttcag
    12661 taataagtcg gaaaaatcgg gttgtaataa tttcttccct ttataagtcc atccaagtct
    12721 ttccagctca ttacgtaatg tatttttttt aatagtatat ctcaagaact cctgcaatgt
    12781 tatttcaaaa gacctttttg cttctgactc tgtcaatcca taccccgtaa tttccaaacc
    12841 aggtgaataa acaataatat tcttctttct ttaaattgaa taagagaaag tgtgcatttg
    12901 atatgtttga ttttttccat gatagtatgt gtaaaaatag gataatttaa aaagaaagta
    12961 caaataaaaa gtaaatactc aacatcgtat tacaacaaac cctgcaatct gaatagttca
    13021 tcgcgcaggc gggcggcctc cataaaatcc atctgcttgg atgctttctg catggctttt
    13081 tttacttttt ctatttgttt gtctaaggct tcagcagtgc cgtattctcc cataggttca
    13141 gctgccacca ttgtttcaat aacttcttct tctttatctt gtacgtttct tcgtatatcg
    13201 agtatcgtac ttttgttggc tatttcttct ttgcttttgt aaatggtttt cggggtaata
    13261 tggtgttcaa tattataagc aatttgcttt tctctgcgcc tatcggtctc gtctatcgtt
    13321 tttttcatgc tcccggtaat gcggtcggca tacataatta ccttaccgtt ggcattgcgt
    13381 gcagcccgtc ctgctgtttg tgtgagtgaa cgtacattgc gtagaaatcc ttctttatct
    13441 gcatccagta tggctactaa acttacttca ggaatatcca atccctcccg caacaagttc
    13501 accccaatta atacatcaaa ctctcccagt cgcaatcctt gtataatttc cacacgctcc
    13561 atcgtatcaa tatcactgtg cagatactta catttgatgt tgagccgggt aagatatttg
    13621 gtcagctctt cactcatgcg cttggtaagt gtcgttacca aaacacgttc atttactttt
    13681 atgcgttcct gaatttcatt cagtaaatca tcaatttggt ttacacttgg gcgaacatca
    13741 ataggaggat ctaataagcc cgtagggcga actacttgct ctacaaattc accttctgtt
    13801 ttcatcagct cataatcgcc gggtgttgct gatacataaa ttgtctgatt caacagtgat
    13861 tcaaattctg aaaaattcaa aggtcggtta tctaaagcag atggcaggcg gaagccatat
    13921 tctactaaat tttgcttgcg gctgcggtcg ccaccataca tgccacccac ttgcggtacg
    13981 gttacatgac tttcatctat cacgcataag aaatcatccg gaaaataatc aagcagacaa
    14041 aagggtctgt cgccgggatg gcggcggtca aaaaatcggg aataattttc aatgccactg
    14101 caatagccca actctcgtat catttccagg tcaaaattca cccgttcatc cagtcgtttc
    14161 gcctcctcgt attttcccat ttcattgtag aattttatct ggtcaaataa ttcatcctgt
    14221 atctcatgga taatttgagg caacatgttt tgtggagcga tataaatatt ggcaggaaaa
    14281 atggcaatgt ctttcatttg ctcgataact ttaccggttg caggattaaa tgattccaac
    14341 tcttccacat catcccccca gaaggtgatt cggtaggcat aatcggcata cgctaaaaaa
    14401 acttcaaccg tatctcctat aactctgaaa tttccacgct gaaaatctgc cgaagtacgg
    14461 gcgtaaagaa tagaaaccaa ttgaaacaag aaagtattcc tgctaatggt ttgtcccttg
    14521 gaaatccgga tgatttggtt cttaaattcc gccggatttc ccaagccata aatacaagaa
    14581 acagaagcca ccaccaaaac atcgcgcctg ccactcatta aagaagacgt tgtgcttaag
    14641 cgcaattttt caatctccgc attgatagac aagtcttttt ctatatacgt atttgttgtt
    14701 gggatatagg cttcgggttg ataataatcg taataagaag taaaaaattc tatcttgtta
    14761 tccggaaaaa attctttgaa ttctgaatat aactgagcca ccaatgtttt attatgcgat
    14821 aaaaccaagg taggacgttg cattttttcg accacgttgg cgatggtaaa tgtttttccg
    14881 gatccagtaa cacccaataa tacttgactg gtttcgccct tatctatgcc cagactcaac
    14941 aagcggatag cttcgggctg gtcgcctgcc ggattaaatt tagaatggat tttaaattcc
    15001 attttcaaaa ataaacaaaa gataagaaca gcccataagt attttgaaag gctgaattaa
    15061 taaaagtaat tacttagatt taggtagagt tgcttatgag aaaaataatt acactattat
    15121 tattaatgca aattgtgcaa tttctgcaag cagaagattg tacgaatggc agatatattc
    15181 gtcaggtttt ttctcgtgtt caagttcaca agaacatagt atatgcccgc aaaatacaga
    15241 gtaatggcgc acctatttat ctccggtatg atgtgtatga gccttatggc gataccttgt
    15301 ccgaacgtcc tgtcatgttg ttaatacacg gcggagcata tttgaaatta ttagatcaaa
    15361 atagcccgga tatagtattg atgtgtgatt attttgccaa aagaggatat gttactgttt
    15421 ctatagatta caggcaagag tcaaactttc tttctatact atcagcagaa aatatgacga
    15481 aggcggtggc tcgtgcgtta atcgatacga gagatgccgt ggatcatttg atgcgcactt
    15541 ataccaatgg caatccctac cggattgata cgtcgagagc atttatcggc ggagtatcgg
    15601 caggagcggt atctacatta tttatcagct atctggacag tctgcaaatg ttgcccacac
    15661 aaatgcagca atggataatt gaagctaccg gcccggaatc agattctatt ttgcgtcacc
    15721 gatttgattt ggttaaaccc aaagctgcga tttctgtatc aggagcattg ttggatacgg
    15781 gatggttggt caataacaat attgatttat tgctgattca cggctctttg gataatattg
    15841 tgccgtataa ttatgactat cctttgggcg tacccatgtt gccaaaatta ttcgggggta
    15901 aagcactcta tcccgtagcg cagcgtcgag gtatccgcac tgagtttgaa gactggattg
    15961 gctatggtca tgtgcccttt atgaatttag atttgggaag tatcattact ttacatctta
    16021 tttcccccga tagattagac agtaccgaaa ggcatattgc acaattttta taccgacatt
    16081 taggatgcaa tttggtatca ggagtacgaa cgaatctgca tgcagaaaag ctgaatatat
    16141 accccaatcc tgcaacatct tattttaatt tgcctttgcc aaaagatgaa actataaaca
    16201 agtggcaact tgaaatatat tctttagacg ggaaattaca actggcaaaa gaagtgaatt
    16261 caaatgtggg aaatataagg atagatgagc tacatcagag cggaacttat atcattaaac
    16321 tatcagtcgt aaaaaataat gaagagcatg tttacatggg tgaagtgcag gtattaaaat
    16381 aagcacctaa tccaaataat cctgataata ttcgtcgaca tattctttta aacaaacttt
    16441 ccagtcacgc atcacgttga gctggcgaag attgagtttc atgttgatta atcgctcgct
    16501 cggcggacgt tgagaaaaat attcttttgc ccaaaagtca gaatcaacgg cagtaatttt
    16561 tatttcattt tctttatcca ataaacgcac taattcggtg gccacttcta aacgtcctgt
    16621 tgtgccgcca caaaccatat tatacaaacc ccatacttca ttttgaagca aaacttttac
    16681 atttctggca aaatcatacg tcaaagtagg tgtacctaat ttatcattta caataaataa
    16741 ttctttcttg ccgtctttca attgttgcat cagcttttgt ataaatttct tgtctttttt
    16801 tggtcctgcg cccatcatcc atccggcccg gcaaatcaga tgttgcgttt tattttgttg
    16861 tacaaatacc tcgcccgccc atttactgcg tgcatagtgt cccagcggat tgggaatatc
    16921 ccaatcgtca aatgtatctt tacttccgtc aaaaattccg gcagtagaaa tataaagcat
    16981 cggaatattg agtgtgttgg aaatatgcac tgcattttct accgaagtag tgttagtcag
    17041 ataagcatct tcaatattgg tatcacaata ctcaagagaa gtatgtgccc ccagatgaaa
    17101 taaataatcc ggtttaaagt caaagacatc ttttttatac gcttcaaaat cgcggaaatc
    17161 taaaaatgac aaccaagtat catttacatc tttatccgtg catttcagct cataatcatc
    17221 tttgaagatt ttataaaatg cttcgcccaa catgccaccg catcctgcta tataaatttt
    17281 ctttttcatt cagaccaaac tttttagaac acccaaatat acgggtttgt ttgcttctat
    17341 cgaaaattta tccaaaacgg tttgacgtgc atgttccccc agccgtcttc gttcttcggg
    17401 ataatcaatc aagtagcgca gtttttccag ccattcttca ttggttttta ctaaatatcc
    17461 atccacatta tctgtcatta ccctgttaat ggcggcacca acattagtag ccactacggg
    17521 caatgcaaat gtcatgtaag ttaatgcttt caaaccgctt ttgcccataa cccacgcgtc
    17581 tgttggaagc gggtatacac cgatgtccat accttgcagt tgtttggctt catcttcttt
    17641 attccaatct aaatattcat aatcgacgcc ttccatatga aagttaaaat tgccgataac
    17701 tcgcaatttg aatgctcgct cttttgctaa ttgctgaaat acttctgtca ataaatataa
    17761 atagggaaca gtagaatgtg tgcctgtcca accaatcact acaggcttat cattcgtgta
    17821 agtattcacc ggaaacatgc gcaatgtgtc caaggtagaa gaaatatccg tgcaatgtgc
    17881 gcaatattgt aaagcgaaat cattcaaatc aggggtacag gtaataacat gatcggcata
    17941 tttcatcatg gcaatagatt tgtctttccc ctttagcata taaattagtt gattcatagc
    18001 agaaccttcc gatttatata tcatgtcatc aatgtcataa acgattttct tagacagctt
    18061 acgcacgagc cactcgaaca tgggtggtcc taatggcgtt acccaaagat gcacgtaaac
    18121 gatgtcatat tttcgcaaac gcaataaatc aagaaagcga cgaacatatc cgcgaattgt
    18181 cccggtgatt ttttggaaaa taaagccggg tttataaata acttcccaaa aactatcatc
    18241 tacaaaagaa ctggtaaccg gatgaaaccc atgctcatca aaataatcga aatactgctc
    18301 atattttaac cgctgactgg cagctttgcc aaaaggataa ggcgaaagaa ataatacttt
    18361 gtgttttttg tttaattctc tactcatgat ttctcagcaa taatcaaaaa ttgctgtcct
    18421 agtattttcc agaataccgg aaatttaaaa tacattttag caataaaact atttagagga
    18481 agtaatcgat tgcctctaaa tgaaagagga aggaatttgg gatatacttt tactatttca
    18541 aatccggcac cataaagatg ttcagctaca cctaattctg tcaatattac agtatgatct
    18601 gcaaaattaa aataatcttt ataggtgtgc ctgaaattgg gtcccatgat tacaatcctt
    18661 ccgccttttt tcagtatgtt atacattcgt tgcaaaaatg cagcgacttc agattgattt
    18721 gctaaatgtt ctaaaaaatt agatatgaaa attccgtcaa aataattatc aggcaattcg
    18781 acattcaagt tattaccaat tatagtttta acattcttgt ctgcatgtag ttttacgcct
    18841 tcatctaaat ctacagtcca tttttctgaa gaaggaacag cattgataaa ttcacataaa
    18901 ccaccggcag ggtcgatgat tttttcggga gcgtttaaat gtttagtgta cagaaattca
    18961 gcaatttctt tccatacgag taatttcctt tctttatcaa catctttaaa tctatattca
    19021 taaattctct tgtaattcat ttagtttgtt taaacttaag taattatttg tcagtaccga
    19081 aaatagtatt tttttagtac tattgcttat tcaaaatatg aaaaaaataa gacaagcaga
    19141 tttatctaca gattatttgc aagttgcaca agatgtgaat acgaagttga cagaactttt
    19201 aacttcgggt acatttatag gtggtcagga agtaagtgca tttgaacgag ctctttcgga
    19261 atatctgggt gtaaaacggg tcatatcttg tggcaacggg accgatgctt tacaacttgc
    19321 gctaatgtca ttaaatctgc caaggggtag taaaataatt gtcccggctt ttacgtatat
    19381 cgcaccaata gaggtgatta aattattggg atttgagcct gtatatgccg atgttgatgt
    19441 gcgtacattt aatatatgct taagcacaat tgcagatgtc tatacggaag aggtgaaagc
    19501 gattgtggtt gtccatttat tcggacaact tatttcggat atagatgaaa ttgcaaaatt
    19561 ctgtgaccaa aagcaaatta ctctgattga agataatgcg cagtctttag gagcagaaaa
    19621 gaaattaaac agaaattcta tttgtacgac atcttttttt cctacaaaaa atttaggttg
    19681 ttatggcgat ggcggtgcca tatatacaaa tgatgaagct aaggcaaata cgataagaat
    19741 gatggccaat catggtcagt caaaaaaata ttatcacgat attattggga taaattcgag
    19801 attggatgcc ctgcaagcag ggatattgaa tgtaaaactt cccggtattg atcaacttat
    19861 ccaaaagaaa aaaaatatat cgtatattta cgatgaatgc tttaaaaata taccttttct
    19921 aactatacct attcaagaaa gcatacatac ttttcatcag tacactttaa tcgttgatgc
    19981 aagatacaga gatgaattac agaattatct ggccgaccat catattgaaa gcaccgttta
    20041 ttatcctttg cctgcctata aacaaaaggc ttatttgaag gatgtttttt taccggtttg
    20101 cgagaactta tgccaaacgg ttttgtccat tcctattcat ctttatttaa gcgaatcaga
    20161 aatcagttat atttgtgaaa caatcgttga attctataaa aacaaaagct gatatgtctt
    20221 attttaaaca cgaaagtgcg tatatagatg atggtgcaag tatcgggaag gatactaaaa
    20281 tctggcattt ctgtcatatc atgcaggcac aaatcgggga gggttgtgta ttaggacaaa
    20341 atgtttttat agccaataag gttattatcg gcaacagagt gaagattcaa aacaatgttt
    20401 ccgtttatga aggagtggat ttggaagatg atgtatttat aggacccagt gtggtgttta
    20461 ccaatgtcat caatccgcgc agttttattg aacgcaaaga agaatataaa tacactttag
    20521 taaaaaaagg ggcgaccata ggtgccaatg taacggtggt atgtggtatc actattggcg
    20581 aatatgcctt tgtgggtgcc ggaagtgtgc tcactaaaga tgtaccggct tatgcgctaa
    20641 tggtcggcaa cccggcaaaa caaaccgggt ggatgagtga agcaggtggc aagctaatat
    20701 ttggttttgg acaaaaggca acttgccccg tgaatggaga gctgtatcga ttacaggaag
    20761 gagttgttat caaagttact gaataaaaaa tcccgcaata actgcgggac tttcatataa
    20821 tgattggtgt ttgtcggatt attttacacg ctccatataa tcgccggtat ctgtgttcac
    20881 ttttatttta tctcctatgt tcacaaataa aggaatcatc acttttgctc cggtagaaac
    20941 cgtgcatggg cgaagtgtat tagtggccgt gtcgccttta attcccggct ctgcataaat
    21001 tacttcaaca actacagtgg ccggcaaggt acaagttaaa ggtaattcat cttctgctct
    21061 taccgcaatt ttcacaatca tgccttcttc cagaaattca ggggcattga taattgcttc
    21121 cggaatggca acttgttcgt atgtttcatt attcataaac atgtactggt cgccttcttt
    21181 atataaaaat tggtattcgc gatgttctac acgcacttca tccagtttcg ctcctgaagg
    21241 aaaggtgttt tccaatactc tgcccgaagt cacacttttg agtttcgtgc gtacgaatgc
    21301 cgcacctttg ccgggtttca catgtaaaaa ttcgacaacc tgataaatat ccgagttgaa
    21361 attaatacac atgccgttgc gtatatctga tgttgaagcc ataaactgat tatttttagc
    21421 gggttcatta agaaaaggaa atcgtcctat tatctcaact ttgcctttta ttttacgtat
    21481 ccttcaatca gtactttgcc gttataatac aaatcattac cgtgtatata agcacggtgg
    21541 ctcaagtggt tttcacctgt ttttttacag gttgacacgt taggagcttc cattttgtaa
    21601 tgtgttcttc ttttgtctct gcgtgtagaa gaatgtcttc gcttgggatg tgccatgacg
    21661 ttttagttta atttatcttt taatttattc aattccgccc atctcggatc atccgaaggc
    21721 gtatcatttt catgttgttt cttttccagt aagctcacga tttctttgtc acaatattct
    21781 gtcttgccgg gcttgtcgca aattctgtga atcggcaatg tcagatgcaa atagtcatac
    21841 accaaagcag taatatcgat atcctgatca tctttgccga tgtataaaat atctatatct
    21901 tctgcttttt tcatttcttc ggtcaatgca tatttcacat aaataacaaa agaagtatga
    21961 acggctaacg gaatttctgc cgtacatctg tcacactcgc tccaaacctc tccctctagg
    22021 tcaacttcca gtaagatagg ttcttgtctt ttatccatgt tgatatgaac atcgatattg
    22081 catttttgaa tttgcgattt ttcaaaatct gtaaagaact tttcattaat cccgaaagta
    22141 aattcgtgga tttcttttct taatccggca tgaggaattc ttatttcgtt caatacatca
    22201 cccatatccg tcaattagga ttaaaaaatt cggattgcaa agataacatc ttatccacaa
    22261 aaatggaaac aattcagaat ggaaatttca gctttttgtt ttttgtaaat ttatgaaaat
    22321 caataattta tatcaaaaac acaggaatta atgtttgggt aatctcattt tttactcaaa
    22381 caggacttta gtttttgaat attttaaccc gatgtccgtc tttttcgtca tcaaaaccga
    22441 taaaataaat tccggaagcc aagtcagcca aattaaccat tctgttgttt acaggacgtt
    22501 gggctacttt agcacccgtt gaattataga tattgacagt cttaaattct tgctgggcat
    22561 gtatgtacaa aatatgagtg gcaggattgg gatataatac caattccttg ctgcttttaa
    22621 aatagataaa cagaatcttt gaatattcaa aagtggaatc aaagtctact tgttttaaac
    22681 ggtaataatt ttcacctaaa aagggttgtt catctaagaa agtgtaagag tgttctatcg
    22741 tgcttgtcgc atgaccatct gcccagccta tattttccca atgaatattg tctgtgctgc
    22801 gctgtatttc aaatccttta ttgttgattt cacttgctgt tttccagttt agtctcacca
    22861 tattgttttc ttctgccaaa tcgaaggtga gaagttcaac aggaagaccg gataccggtt
    22921 ttatttttat ccaaaccgga tttgttaatg catgaaaagt ttcattggtt aaaatatatg
    22981 gcgatcctgc accgtagttt tttgttgtac tcagtgcttc gcgaatataa aaatattgat
    23041 ccataggaac gtttgcagca ccaaacagac taataatttt tgatagggaa agtgatgccg
    23101 atgttcccgt aacaggataa cttactgtgg tttctcccga agaagttccc aaaattaaat
    23161 ctatactagt gattgtgcca tattctgcag agcttacata ttgcagattg agaaataatt
    23221 tattggtatc cgtagtttgt acaataaaat catcacccat gaatttctca tttaccgtat
    23281 tattcccatc tgtgctgaag ccttgtacta tcaaaggacc gtctgaaatg gatatatttc
    23341 catcatacat ggattttaaa atattggtgc cgtttgttcc cattccggtt ggcgcatagg
    23401 ctaatgtcat taatttgccg actgcattat tttgtataga tccgccaaga tttcctaaaa
    23461 atgattgcac gtaattagtg
//
LOCUS       DAOQII010000005        22493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1037_length_22493_cov_5.512523, whole genome shotgun sequence.
ACCESSION   DAOQII010000005 DAOQII010000000
VERSION     DAOQII010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22493
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1037_length_22493_cov_5.512523"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1144)
                     /locus_tag="PLF48_00555"
     CDS             complement(212..1144)
                     /locus_tag="PLF48_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47838.1"
                     /translation="MIGFVFISFSQTKTTKKQVAYFRSIEYFNTEDSSKDYAVVYRPN
                     QAPKALLILLPGFGESPQLAERETNIPEYAATAGILTLIISNTDGNTSFYIDQKAQQY
                     LDEMIPEWLIKYNITSGKYYLGGFSLGGSGVVKYVQHCQIYDIPQKPTAVFAIDPPLD
                     FYRMYKVYDKWLNDTNISYLSKKQYLVFMDKMKVYFKGSVSDAYDNYMRLSPYSYEDK
                     DNIGARLFGNTPIQIYSEPDFNWTLNEKHWDIYDLNIIDCESFINHLQKNGNKNASLV
                     ITQNKGKRKLLNIRHPHSWSIADGKDVVNWLMKY"
     gene            complement(1502..1873)
                     /gene="crcB"
                     /locus_tag="PLF48_00560"
     CDS             complement(1502..1873)
                     /gene="crcB"
                     /locus_tag="PLF48_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNF47839.1"
                     /translation="MKKAILLVGLGGFAGSICRYLISVYISKYIPSSFPYGTFIANIL
                     GCFIIGLIFGLGIKQSTLSPEWRIFLATGFCGGFTTFSTFAYENVKLLQDGYFSTFLI
                     YSFATFLSGILAVYVGMMLNR"
     gene            complement(1885..2376)
                     /locus_tag="PLF48_00565"
     CDS             complement(1885..2376)
                     /locus_tag="PLF48_00565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF47840.1"
                     /translation="MDKKKVALIFIIIFSSITLRQILFNTPKMPLDKTTLTFENGTIF
                     EKSALQNKVSIISFYQTWCGDCRAEQKDLLQLHQQYGDKLQILFVSDEEWAKVDEMKN
                     HLQTTDLTFLKSDKSLKSIGIRRFPTTYLVDKNGKVRLNKVEAKDWNTPEMKKIIESL
                     IAK"
     gene            complement(2369..3502)
                     /locus_tag="PLF48_00570"
     CDS             complement(2369..3502)
                     /locus_tag="PLF48_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47841.1"
                     /translation="MNSARHLHIVCFDIPFPADYGGAIEEWYKIKTLAQLGIKIHLHC
                     FEYNGRKPDKEIDSVCEKVYYYKRERKLTDALSKIPFIVKSRMQDELLQNLCKDNYPI
                     LFDATHTTGFINHPALQERKKAVRLHNIEWIYYRVLLADAVSLKEKIYYFSEYKKLKK
                     YDSVLANADFLSCLSQTDLEYYQTMFPEKSISFEAVFHENTTVTAIPGRGDYILYHGN
                     LSLTDNYAYVIELIEHQLKPSEHQIIIAGKNPDEALRNCIAKYAHITLVANPNQDELA
                     LLVKNAQACLAFARNPSGVKLKLINSLFQARFVIANEAALTGSNLSDLVISCETEDLN
                     SVINQLMQQEFTTEIIEKRKNKLLSLYNNQSNAEALIKTIYHG"
     gene            complement(3499..4590)
                     /locus_tag="PLF48_00575"
     CDS             complement(3499..4590)
                     /locus_tag="PLF48_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF47842.1"
                     /translation="MKNRIILTVTNDLVFDQRMQRICESLQKEYSVELIGRLLDASPA
                     VDTKPYLQTRLKCFFNKGFLFYAEFNLRLLFYLLRFPFDIICGCDLDTLPASVLAGKI
                     KRKKIAYDAHEYFPESPELVHRKAVKKIWQLIEKVFVPHTDLQYTVCDSIAQLFIVKY
                     KKQFEVIRNLPYYTFQENTNAVKSRIILYQGAVNVGRGIKEMIAAMKLLPYFKFYVAG
                     DGDILEDMKKYVRELQLQDKIKFTGKLKPNQLKQLTNEAFIGVNILENRGLSYYYSLG
                     NKTFDYIQSDIPQVLISFPEYKKINDEYAIGVLVNDMDATTLSTAILSLKENQDFYKQ
                     IQSNCVRAAKDLCWENEEIKLLNLYTSIS"
     gene            complement(4587..6491)
                     /locus_tag="PLF48_00580"
     CDS             complement(4587..6491)
                     /locus_tag="PLF48_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNF47843.1"
                     /translation="MLLPIHILEKYWGYTAFRYPQQEIILSVLDGKDTLALLPTGGGK
                     SVCFQVPAILQDGICIVVSPLIALMKDQVYQLSKRGIKASAIFSGLSYNEIDKILDNA
                     VFGMYKLLYVSPERLKTELFIERFKQMKVNLIAVDEAHCISQWGYDFRPPYLEIAEIR
                     QYHPDIPVLALTATATQKVQDDIIEKLHFRDSNIFRKSFVRDNLSFVVRHELAKLPKL
                     VEVIKKLKGSGIVYVRNRKQTKEIAEYLKKNKLNADFYHAGLSTDERNAKQDNWIKNK
                     TQVIVCTNAFGMGIDKADVRFVIHFDIPETLEAYYQEAGRAGRDGKKSYAMLLYEQKD
                     IDNLLQKLEQKYPDEQTIKMVYNAVCNHLEVAIENGAMHTYFFDLLYFCKLFKLNAAL
                     VYNALKILEQQNYMQLSEGVELASRIIFRVDKSEVYRFEVAHSVYSPLIKSLLRTYGG
                     ILDHYSKISEEQLARLIGQPKEVVVKQLLYLHKQKLLTYIPSSDKPTITLLTERLHEN
                     NLLIDSRFIQLRKEVQEAQLKAMLNYTEQDKVCRQVVVCVYFDEKDVPPCGKCDVCLE
                     QKNKLDADKNFESIKVDVLGKTKNNFIPIEKIIPENAFYKKEQYKKVIRFMLDERMLM
                     MNEQNEIKAV"
     gene            6904..9330
                     /locus_tag="PLF48_00585"
     CDS             6904..9330
                     /locus_tag="PLF48_00585"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF47844.1"
                     /translation="MNIKKIRLFPFLLCLFMLLNSVYIHSSNIKGNIIDEKNRPIEFA
                     NITLFSAIDTVVVSTTFSDSSGFFELLNIHRGDYFLEISYLNYKKDSLLLSAFEDSVE
                     IGTISLQPLANNLDLVTITANKPIYERKADRIIFNVENSVYSQGSDAMLALSKAPRVQ
                     INNNDIKIAGRGDAAVLINDRLIRMSGEELSQYLKSIPSNDIQKIEVIPNPPAKYDAQ
                     GAALINIVLKKNRKQGYNGSVSGNFQQHQYQGGRIGTTINFTKKKMSFNSSISDQINK
                     GYREETHILKYSEQTWKDTAINPQLSNTFSGRMSMNIDINNNNTFGFNYTGSWSKVIW
                     DKETINTRIRDNINATLIQSTKNEANSNRNFYNHNITAYYTLNIDTMGKKLDIGFDFF
                     NSYRNINRNYTNATFNGNDSLLSQPIPQENTNGTQKSYIYTINADMEHPIKYGTWQYG
                     TKFTYFYVRSNNEQSVLQPDNTYQLETTRSSNFSYKEFNEAIYGSFSKSFKNVELQAG
                     IRIEFTQLKGMLHTTGETNNQQYIRPFPSLMIQYKKNDKHQINFSINSRIDRPAFWQL
                     NPFRYYNTQYSYAEGNPYLQPQYSYNTSLEYVLKQNYSFSVYYNYTLNNSTQIQFTDT
                     SNNTNFWQWEAKGRQMHDLGTFNQFYFSIKEKWQPTIAIDVFIDFLKSPYLYNNTKRT
                     SWGMALTINNDFNFDKNGRLTGSLNYYFTPPAPRQELFMRMYNKIDIGIKAKLLKEKL
                     LSLSFFANNILRSKQSNGYFINTTGQYSSFTNYYNDRVFTFSITYKFGNKNLKTINKQ
                     TDNENISRAK"
     gene            9478..9675
                     /locus_tag="PLF48_00590"
     CDS             9478..9675
                     /locus_tag="PLF48_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47845.1"
                     /translation="MKKNQKKIDLSNFEKLSEASTGSLVGGFSVAKSGPSTNLKAAVN
                     LIKCTTTNNCDGGNCISGCGG"
     gene            9742..9987
                     /locus_tag="PLF48_00595"
     CDS             9742..9987
                     /locus_tag="PLF48_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47846.1"
                     /translation="MKNKLNFARLNNSEAAQLIGGFSSVFSGNFSCGDTSKINNCYGG
                     NLVVGCGKGFKSKKSSKHIPNTNCKGNCVKGCGEKTK"
     gene            9994..11307
                     /locus_tag="PLF48_00600"
     CDS             9994..11307
                     /locus_tag="PLF48_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNF47847.1"
                     /translation="MKLSQFNSIIPYNDNYALYNSYSNKVIIIEKELKDLLDAARSEN
                     IEELINIHPTFYNYLIETEFVVSDSINEVENVKIISKKIDENKNAFFLTINPTMNCNF
                     KCWYCYETHIKDSRFTSEMIIKINKFISNTVSDSQIEQFTLSFFGGEPLLFFKKDVIP
                     VISHFTEICNKHNISNNISFTTNGFLINEEIINYLKELNTTCSFQITLDGFNEKHDLV
                     RFVNNKRGSYNTIINNIKLLINNCFFVRLRVNYTNENISDAYKIADEFNDTESNLKES
                     YLLFDYHRVWQDDKIDDINIQLDYNKKYISSKNINILGNYSPDNVVSSCYADKRNSVV
                     INYNGDIYKCTARDFTLANRAGYLSNKGELIWENNSLEKRMNSKFNNKPCLSCRLLPI
                     CNGGCTQHAIEHLNKDEYCIYFGDEAEKDKVIKSKVDDVLQYIEI"
     gene            11279..13483
                     /locus_tag="PLF48_00605"
     CDS             11279..13483
                     /locus_tag="PLF48_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNF47848.1"
                     /translation="MMFYNTLKFNRNKFVHQLESTDCGPACIVMVAYHFGGKYTLTQI
                     KDICEITRMGVSVQDIIDGAKKINFNTTALKLTIEDLEQIPLPSILYWKQEHFIVLRQ
                     IKNNNVYIIDDPAYGRVKMSQKDFVSEWIGNNNCKGIAIVLEPNGELTKIDTNKPNRV
                     SESLKLVFNFINKNKIKYTIAILLLFVALACNWAIPIFFQKTIDNGIVNKSIQIITYL
                     LLAQFVLFISHIISDTLSHILLVKVNFHLSILLQNSFLSKLIKLPINYFDTKLNTDTL
                     QRISDQHIIQEFTTWKGIELFINTLNIIIFGGMLLSINYTIFNLFIFLSLISIFWTFL
                     FLKSRANLEYSIFIQQSLSNNNMYEFIMNMPEIKINGAQNNLINKIKTIQQKLNNLEL
                     KSLSLNTYQVLGTSFINKALEIIAIAISAYFIIESRQTLGQLLSISYILGQLSSPIRN
                     IINFTKDLQDALISKNRLDEVYNEQNEDNENKIINFNHHSNLIMRDVSFKYPGSFNSF
                     VLKNIDLIIPKNKKTAIVGVSGSGKTTLIKLLLKYYDIQNGELLLGSENVNLLNAEKW
                     RELCGIVLQDGSIFSGTIIENIAFSDDTVNIDKIKKATKTACIDKFIESLPLQYNTKI
                     GNIGIQLSGGQKQRILIARAIYKNPDFLFFDEATSALDAKNEKEITDNLEQFFKNKTV
                     VIVAHRLSTVKNADQIVVLDGGEIVETGTHEQLVKNKAHYFNLVSNQLELGN"
     gene            complement(13642..15066)
                     /gene="gatA"
                     /locus_tag="PLF48_00610"
     CDS             complement(13642..15066)
                     /gene="gatA"
                     /locus_tag="PLF48_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNF47849.1"
                     /translation="MAHSYRTLAALQQDLLNKTLTCTEITQNYIHKIKEQSHLNAFNE
                     IFETTALTSAQNIDNKIAQGLPLGKLFGCVIAIKDNIVYNNHLCTASSKILENFISPY
                     SATVIERLLKEDAIIIGRTNCDEFAMGGSNENSFFGAVKNPHDTTRVSGGSSGGSAAA
                     VAADLCFAALGSDTGGSVRQPASFCGVVGLKPTYGRVSRYGLIAYGSSFDQIGPLTNT
                     VEDAALLLEIISGADEYDATCSPKAVPTYSKHLHEHKKYKIAYYKNAVEHENIDTEVR
                     DACLKAIENLKAEGHEIQAIEFPELDYIVPAYYVLTTAEASSNLSRFDGVKYGYHAKE
                     ATSLEETYILSRSQGFGKEVQRRIMCGTFVLSAGYYDAYYSKAQKVRRIVAEATYKTL
                     EEFDFILSPVAPTAAYKIGEVSDPVASFLGDIFTVQANIAGIPAISLPLHQTANGLPI
                     GIQFMAGRWQEEKLLGFSKQVLGV"
     gene            complement(15082..15303)
                     /locus_tag="PLF48_00615"
     CDS             complement(15082..15303)
                     /locus_tag="PLF48_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNF47850.1"
                     /translation="MYNLLSFLNLGGGEIFLIFAAILLLFGGKKIPELMRGIGKGVKE
                     FNSAKANLQDEFEKGMKDEDVKSSDKTAQ"
     gene            complement(15601..15978)
                     /locus_tag="PLF48_00620"
     CDS             complement(15601..15978)
                     /locus_tag="PLF48_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNF47851.1"
                     /translation="MEAEKTRIDKWLHAVRLYKTRTLASEACEAGKVKVNDEKVKSAF
                     QIKVGMLMELNKQGVRYKYKVLKIIEKRVSAGIAQQCYEDLTPLEEIDKLKFPAFFYE
                     VRDKGVGRPTKKERRDIDKFKGQ"
     gene            16047..16484
                     /locus_tag="PLF48_00625"
     CDS             16047..16484
                     /locus_tag="PLF48_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47852.1"
                     /translation="MRKIILFGIIIIALGAFAKKPPKKTKTPTASTWVEPVKPKLIYG
                     NWTWFETDCCGSRRGVSTPESSGDNISLELRNDYTFLETHSKKNALPREGNIILFKQD
                     GYNMIQFNDERPARYTLSDNNDTLTLSWKYMELQTEKYIRNKN"
     gene            16498..16908
                     /gene="ruvX"
                     /locus_tag="PLF48_00630"
     CDS             16498..16908
                     /gene="ruvX"
                     /locus_tag="PLF48_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNF47853.1"
                     /translation="MPKILALDYGKKRTGLAVSDESKIFAFGYGTQDTNSLMIYLDGL
                     LQKERIDTLLVGMPKKLNNEDAEISTAIRKFIQLFKEKHTAIIVVEWDERFTSKMAMQ
                     SMVESGMKKKDRQNKATIDEVSATILLQSYLETL"
     gene            complement(16927..17550)
                     /locus_tag="PLF48_00635"
                     /pseudo
     CDS             complement(16927..17550)
                     /locus_tag="PLF48_00635"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
     gene            complement(17553..18185)
                     /locus_tag="PLF48_00640"
     CDS             complement(17553..18185)
                     /locus_tag="PLF48_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNF47854.1"
                     /translation="MNITVIGASAGVGLATVKRALQRNHKVTTLSRSPIQLPASENLI
                     QISGSATNKSDLSKAIQNADAVIIALGTGKSMKATTLYSDFAKLLLELHSENKYRQPF
                     ICLTGFGAGDSGAYNALFMKIFFSLLLKSVYADKTIMEELIAKSDLKWEIVRPGLLTD
                     KPLTENYRTETKLSKGINIGSISRDDVADFMVKQAEQPTYLSQYPALSTK"
     gene            complement(18200..18736)
                     /gene="dcd"
                     /locus_tag="PLF48_00645"
     CDS             complement(18200..18736)
                     /gene="dcd"
                     /locus_tag="PLF48_00645"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNF47855.1"
                     /translation="MILTDKGILDEMQKGTIVIEPYDRAYLGTNSYDVHLGKYLAVYT
                     DRVIDAKKHNPIQEFEIGEDGFVLQPGVLYLGVTEEYTETHAHVPFLEGKSSIGRLGI
                     DIHATAGKGDVGFCNHWTLEISCIQPVRVYAGMPVGQLIYFEVKGDIEHAYNKKQSAK
                     YNQRSPKPMESMMWKNFK"
     gene            complement(19069..>19275)
                     /locus_tag="PLF48_00650"
     CDS             complement(19069..>19275)
                     /locus_tag="PLF48_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47856.1"
                     /translation="KKPRSGIVRIKILNFLNALMVNNSLRYKPRIGKTFIYKTYNLKL
                     SHVMAAPFIGGLVNEFNSLVVVQI"
     assembly_gap    19276..19375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19770..20384
                     /locus_tag="PLF48_00655"
     CDS             19770..20384
                     /locus_tag="PLF48_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNF47857.1"
                     /translation="MSANLQSRIPVMEHFYTLQGEGFYAGTAAYFVRLAGCDVGCHWC
                     DVKESWIVDEKQWMNISDLVDDIKKTATQRVVITGGEPLMYDLLPLTTALKNENYFIH
                     IETSGAYPLSGILDWICVSPKKFKPVLKEVLLAANELKCIIFHKSDFAFAEQNRVLTN
                     EKCQLYLQAEWSKRELFYPEIVAYCKQYPYWKLSVQTHKYLDIP"
     gene            20445..22322
                     /locus_tag="PLF48_00660"
     CDS             20445..22322
                     /locus_tag="PLF48_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF47858.1"
                     /translation="MKKTIAFLFVFLSAYGLFAQQVAEKAMSAYFKAKGYAQFTQWEE
                     GIAELNKAIKIQPDFLDAHALLGEMYYASKKYDSSIIVLQNIIEQPGIPARSYYCLSE
                     SYLKKSEIAKAKQYAQQYLNQPNVQEHFARKAQRTVINGNFAEEAMKHPVPFNPKNPG
                     ANINTKSPEYFPYLTPDGSYFLFTRLENNQEDVYYAKKTDTSFSPAQSFGTNINTFEN
                     EAAAVMNVSGNLLFLTECNKLDGFGSCDIYFSQNNNGNWSKPMGIGKPVNSGAWEAQP
                     SFSSDSKALYFSSNRKGGFGGRDIWVTYLDSAMHFSEPVNLGNKINTPYDDQCPFIFS
                     DNKTLYFTSDGWPGMGAGDIFMAQLTDTGWTNPKNLGYPINTESDDNGLTVSYDGKTA
                     YLSSNRSNGYGGLDIYSFELPDAVRPPSGTFVKAIIMDKKTKQLLPANYRLINLENKS
                     EIINGIAKNGSFFERINANKNYAVQVEKDGYLFYSQNINLKANTQVKPCQLKIELEPI
                     AVNAKITLDNIFFDFDKFDLKSESFADLENLTGILKKNPGLKIEISGHTDHKGDAKYN
                     LNLSQKRAESVLNYLVQQGISASRLTAKGYGDTQPVAPNDTEENRSRNRRIELKVIQI
                     N"
     gene            complement(22338..>22493)
                     /locus_tag="PLF48_00665"
     CDS             complement(22338..>22493)
                     /locus_tag="PLF48_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNF47859.1"
                     /translation="LIGATNPANAEEGTIRKVYAKSVGENAVHGSDSNENAQIEGDFF
                     FSKLERF"
BASE COUNT         7518 a         3835 c         3894 g         7146 t
ORIGIN      
        1 ccgagagtta tcacatttac tttgtctttt ttaagtgttt ttgttttcat tttttggaga
       61 ttggtggttg gtcattggat attagagatt ggtgattagt cattggagat tggtgattgg
      121 ttggttctga tttctgattt cttactaccc aatactcatt actcttttct tgactcttgt
      181 atcatatagc ttacaattaa tcacaatctg ctcaatattt catcagccaa tttacaacat
      241 ctttaccatc tgctatactc cacgagtgtg gatgacggat gttgagtagt tttcgttttc
      301 ctttattttg cgtaatcact aaacttgcat ttttattccc atttttttgc agatgattga
      361 tgaaactttc gcagtctata atatttaagt cataaatatc ccagtgcttt tcgttgagtg
      421 tccagttgaa gtccggttca ctgtaaattt gtattggtgt gttgccaaac aaacgcgctc
      481 cgatattgtc tttgtcttca taactatagg gcgacaatcg catataattg tcatacgcat
      541 ccgacacact gcctttgaaa taaaccttca tcttatccat aaaaaccaga tactgttttt
      601 tggaaagata agagatgttc gtgtcattaa gccatttgtc ataaacttta tacatgcggt
      661 aaaaatccaa tggaggatct attgcaaaaa cagcagtcgg tttttgcggt atatcatata
      721 tctggcaatg ctgtacatat tttactacac cactgccacc caaagaaaaa ccgcctaaat
      781 aatatttgcc cgaagttata ttgtatttaa taagccactc cggaatcatt tcatctaagt
      841 attgttgtgc tttttggtcg atataaaatg aagtattgcc atctgtattg gaaataatca
      901 atgtaaggat gccggcagtc gcagcatatt ccggaatatt agtttcgcgt tctgccagtt
      961 gcggagattc gccaaagccg ggcagcaaaa tcaacaaggc tttcggtgct tggtttggcc
     1021 ggtaaactac agcataatct ttggagctgt cttctgtgtt aaaatattca atagagcgaa
     1081 agtaggcaac ttgcttcttt gtcgttttcg tttgtgaaaa cgagataaaa acaaacccaa
     1141 tcaagcatat tatgaaaaga tacccacgca tatagaatgt ggcaaaagta ataaaaaaca
     1201 aagagagatg ctgtattatg gaatagctaa acccaagttg ataaaatctt ataattaata
     1261 cgttaatctc ttttgtggtt tgatttgtaa tttttaccac atagaggctt agatttttta
     1321 ggattaataa ctcttactat acataaaatt agggtaagaa tagaaaaata taggaatcgc
     1381 caaaggcaat actctatttt tgcttgactg tctaaacaga tttgcaatac gctacaattt
     1441 atgattaaaa atgctatgtt tctatgtggt ttaaaatgaa cagtgggtta tatgattcaa
     1501 tctatctgtt tagcatcatt ccgacataga cggctaagat cccggacagg aaggttgcga
     1561 aactgtatat taagaaagtg ctgaaatagc catcctgcaa gagtttcaca ttttcataag
     1621 caaaggttga aaatgtagtg aacccaccac aaaacccggt ggctaaaaag atgcgccatt
     1681 ccggtgagag agtactttgt tttatcccca atccgaaaat cagaccaata ataaaacaac
     1741 ctaaaatatt agcaataaat gtaccgtaag gaaaggagga tggaatgtat tttgaaatat
     1801 aaacagaaat tagatagcgg caaatactgc ctgcaaatcc gcctaagcct accagtaaaa
     1861 ttgctttttt catgttttgc cgggttattt ggctatcaag gattctataa tttttttcat
     1921 ttcaggtgta ttccagtctt ttgcttctac tttgttcaac cgcacttttc catttttatc
     1981 cactaaatag gtagtcggaa aacgacgaat gccaatagat ttcaaacttt tatctgattt
     2041 taaaaaggtg agatctgtcg tttgtaagtg gtttttcatc tcatcaactt tagcccattc
     2101 ttcatcactg acaaaaagga tttgcaactt gtctccatat tgttgatgca gttgcaacaa
     2161 gtccttttgt tctgccctgc aatcaccgca ccacgtttga tagaatgaga tgatactgac
     2221 tttattttgc aatgctgact tttcgaaaat tgttccattt tcaaaagtta gggtggtttt
     2281 atctaagggc atctttggag tattgaataa tatttgtcgc aacgtaatag agctgaaaat
     2341 aattataaaa atgagggcaa cttttttctt atccatgata aatagttttt attaatgctt
     2401 ctgcgttcga ctgattattg tacaacgaaa gtaatttgtt ttttcttttt tctataattt
     2461 ctgttgtaaa ctcttgctgc ataagttggt tgattactga gttgagatct tctgtttcgc
     2521 aagagattac taaatctgat agattgctac cggttaatgc cgcctcattg gcaattacaa
     2581 aacgcgcctg aaacaatgaa ttgatcagct ttaattttac accgcttgga ttgcgggcaa
     2641 atgccagaca tgcctgtgca ttttttacca gcagagccag ctcatcttga ttcgggttgg
     2701 caactaaggt aatatgtgca tacttggcta tgcaattgcg caatgcttca tcaggatttt
     2761 ttccggctat aattatttgg tgttcgctgg gttttaattg atgctctatc agttcaatga
     2821 catacgcata attatcggtg agtgacagat tgccgtgata aaggatataa tcgcctctgc
     2881 cgggaattgc agtaacagta gtattttcat gaaaaacagc ctcaaaggag attgattttt
     2941 caggaaacat cgtttgataa tattccaaat cagtttgaga taaacaagat aaaaagtcgg
     3001 cattagctaa aaccgaatcg tactttttta gttttttgta ctctgagaaa taataaattt
     3061 tttctttgag agaaacggca tcagccaata atacccgata gtaaatccat tcgatattgt
     3121 gtaagcgcac tgcttttttt ctttcttgta atgcaggatg atttataaaa ccagtagtat
     3181 gtgtggcatc aaataaaatg ggataattgt ctttacataa attttgcagt aactcatcct
     3241 gcattctgct tttaactata aacgggattt tagataaggc atccgttagt tttctttctc
     3301 ttttatagta atataccttt tcgcaaacag aatctatttc tttatcaggc tttcgtccat
     3361 tatattcaaa acaatgcaga tgaattttga tgcctaattg tgctaaagtt ttaatcttat
     3421 accactcttc gatagccccg ccgtaatcag caggaaatgg aatatcaaaa caaacgatat
     3481 gcagatgtct ggcagaattc aagatatgga tgtatacaag tttagtaatt ttatttcttc
     3541 attttcccaa cacaaatctt ttgcagcacg aacacaattt gactgaattt gtttgtagaa
     3601 atcttggttt tcttttaagg ataaaatagc cgttgacaaa gtagttgcat ccatgtcatt
     3661 tactaaaact ccaatagcat attcatcatt gatttttttg tattcaggaa atgagattaa
     3721 tacctgcggt atgtccgact ggatataatc aaatgtttta ttgcccagtg agtaataata
     3781 actcaagccc ctattttcta aaatattgac acctataaaa gcttcattag ttaattgctt
     3841 aagttggttc ggtttgagtt tgccggtgaa tttaatttta tcttgtaatt gcagttccct
     3901 aacatatttt ttcatatcct ccaaaatatc accgtcgcct gcgacataaa atttaaaata
     3961 gggtagcaat ttcatggcag ctatcatttc ttttattcct ctgcctacat tcacggcacc
     4021 ctgatataag ataatcctgg atttgacggc gttcgtattt tcctgaaaag tataataagg
     4081 taaattgcga ataacctcaa attgtttttt atatttcacg ataaacaatt gtgctattga
     4141 atcacacacg gtatattgta aatctgtatg tggaacgaag actttttcta taagttgcca
     4201 tatttttttt actgcttttc gatgaaccag ctccgggctt tccggaaaat actcatgtgc
     4261 atcatacgca attttcttgc gttttatttt tccggctaat actgaagcag gaagtgtatc
     4321 taaatcacat ccgcaaataa tatcaaaagg aaaccttagc aaataaaaga gcaagcggag
     4381 attaaattca gcataaaaca aaaatccttt attgaaaaaa catttcaatc ttgtttgtaa
     4441 ataaggtttt gtatctacag caggagaagc atctaatagt cttccaatca attctacgga
     4501 atattctttt tgcaatgatt cgcaaatgcg ttgcatgcgt tggtcgaaaa ccaaatcatt
     4561 tgttaccgtg agtatgattc tatttttcaa accgctttta tttcattttg ttcattcatc
     4621 atcaacatgc gctcatccag cataaaacgg attactttct tgtattgctc ttttttgtag
     4681 aaagcatttt caggaattat tttttctata ggtataaaat tatttttagt cttacctaaa
     4741 acatctactt taatgctttc aaagttttta tcggcatcca atttgttttt ttgctctaaa
     4801 catacatcac atttgccaca tggtggcacg tctttttcat cgaaatagac acacactacc
     4861 acttgccgac aaactttatc ttgttcggtg tagtttagca ttgcttttaa ttgtgcctcc
     4921 tgcacttctt tgcgtaattg aataaaccgc gaatcaatca gtaaattatt ttcgtgcaat
     4981 cgttcagtaa gtaatgtaat cgtaggttta tcactcgacg ggatatacgt cagcaacttt
     5041 tgtttgtgta aataaagcag ttgttttaca actacttctt tcggctgacc gattaatctg
     5101 gccaattgtt cttcagaaat tttagaataa tgatctaata taccgccgta ggtgcgcagc
     5161 aatgatttta tcaatggaga ataaactgaa tgcgccactt caaaacgata gacttcactt
     5221 ttatctaccc tgaaaattat tctggatgct aattctacac cttctgacaa ctgcatgtaa
     5281 ttttgctgct ctaagatttt aagcgcatta tatacaagcg ccgcattgag tttaaacagc
     5341 ttacagaaat aaagtaaatc gaaaaaataa gtgtgcatag caccattttc tattgcaact
     5401 tccaaatgat tacaaacagc attatacacc atttttatgg tttgttcatc cggatatttt
     5461 tgttctaatt tttggagtaa attatcgatg tctttttgct catacagcaa catggcataa
     5521 gattttttgc catcgcgtcc tgcccgccct gcctcctgat agtaggcttc taacgtttcc
     5581 ggaatatcaa agtgaatgac gaagcgtaca tctgctttat ctatgcccat gccaaaggca
     5641 ttggtacaca caattacctg cgttttattt tttatccagt tgtcttgctt ggcattgcgt
     5701 tcatccgtgc ttaatcctgc atgataaaaa tcggcattca gtttattttt tttgaggtac
     5761 tccgctattt ctttcgtttg ttttctgttg cgcacataaa caatacccga gccttttagt
     5821 tttttgataa cttccaccag tttgggcagt ttagccaatt catggcgcac tacaaaactc
     5881 aggttatctc tgacaaacga tttccggaaa atattgctgt ctcggaaatg caatttttcg
     5941 atgatatcat cctgcacttt ttgtgtggca gttgccgtga gtgccagtac gggaatatct
     6001 ggatgatatt gtctgatttc tgcaatttct aaatatggcg gacgaaaatc ataaccccac
     6061 tgagaaatgc aatgcgcttc gtcaacggca atcaaattga ctttcatctg cttgaatcgc
     6121 tcgatgaata gctctgtctt caaccgctcc ggagatacat acagcaattt atacatgccg
     6181 aaaacagcat tgtctaatat tttatcaatc tcgttgtagg ataaaccaga aaaaatggca
     6241 gatgccttga taccgcgctt agacaattga tagacctgat ctttcatcaa agcaattaat
     6301 ggcgatacca ctatgcagat gccgtcttgc aggatagccg gaacttgaaa gcaaaccgac
     6361 ttaccgccac ccgtaggcaa taaggcaagc gtatcttttc catctaatac ggacaggatg
     6421 atttcttgct gcggatacct gaaagcagta taaccccaat atttttcgag tatgtgaatg
     6481 ggcaaaagca caaggctaaa ttaaggagaa atgcggatat ggagggtggg tagttgaaaa
     6541 agtgatttta gtataaataa agtaaggaaa tcaaaatttt tgcaataaaa tatatgcaca
     6601 ttatgacata agcatagatt aacatatcta tgtcttatag cataaaatgt taatattagt
     6661 aaaatttcaa aaaaatgttc catttttttt ttgattttta tcatttatat ttgacttatt
     6721 aaatgtaaaa tcggggacgg cagtttccgt aagctaacga tgaaaatgag gtgcaatact
     6781 tcagaaaata aggggtaaat aaaataagga caatagtatg ttgttctaat gaatattgaa
     6841 atagaaattt ttcagacttg gaatatttct aattgataaa tttaaataag ttagtaaatt
     6901 gtaatgaata ttaagaaaat aagattattt ccgtttttgt tgtgtctgtt tatgctttta
     6961 aatagtgtgt atatacattc atcaaacata aaaggtaata taatagatga aaaaaacagg
     7021 ccaatagaat ttgccaatat aactttattt agtgccattg atacagttgt tgtcagtaca
     7081 actttttccg acagctccgg tttttttgaa ttgttaaaca tacacagagg ggattatttt
     7141 ttagaaataa gttatttgaa ttataaaaaa gacagcttat tactaagcgc gtttgaagac
     7201 agtgtagaaa taggaacaat aagcctacaa ccattggcaa ataatcttga tttagttact
     7261 ataacagcca acaagcctat ttacgaacgc aaagccgata gaataatatt taatgtagaa
     7321 aatagtgtgt atagccaagg cagtgatgcc atgctggcat taagcaaggc accgcgagta
     7381 caaataaaca acaacgatat aaagattgca ggcagaggtg atgcagcagt tctgataaac
     7441 gatagactaa tacgaatgag tggcgaagaa ctaagccaat atttaaaaag catacccagc
     7501 aacgatatac aaaaaataga agtaataccc aacccacctg ccaaatacga tgcacaaggc
     7561 gcagcattga taaatattgt attgaagaag aatagaaaac agggttataa cggaagcgtg
     7621 tctgggaatt ttcagcaaca tcaatatcaa ggaggaagaa tagggacaac aattaatttt
     7681 actaaaaaga agatgagttt taattcatct atttcagacc aaataaataa aggatataga
     7741 gaagaaacac atattctgaa atatagcgag caaacttgga aagatactgc tatcaatccc
     7801 caattatcta acactttttc tggcagaatg agcatgaata tagatataaa caataataat
     7861 acatttggct ttaactatac tggctcttgg agtaaagtaa tttgggataa agaaactata
     7921 aatacgagaa tacgtgataa tatcaatgcc acgttaatac aaagtacaaa aaatgaagct
     7981 aatagcaacc gtaatttcta taaccataat attactgctt actatacttt aaatatagat
     8041 acaatgggta aaaaattaga tattggattt gattttttta atagttatag gaatataaat
     8101 cgtaattata ctaatgccac ttttaatgga aacgattcat tattatcaca acctataccg
     8161 caagaaaata caaacggaac acaaaaaagc tatatctata ctattaatgc agacatggaa
     8221 catcctataa aatatggcac ttggcaatat ggcacaaaat ttacttactt ttatgtaaga
     8281 agcaataatg agcaaagtgt attgcaacca gataacactt atcagttaga gacaacacga
     8341 agtagcaatt ttagttataa agaatttaat gaagccattt atggtagttt tagtaaaagc
     8401 tttaaaaacg tagaattaca agctggtatt agaatcgaat ttacacaact aaaaggcatg
     8461 ctgcacacta caggtgaaac aaataatcaa caatatatac gccctttccc cagtttaatg
     8521 atacaataca agaagaatga taagcatcag ataaatttct caataaattc taggattgac
     8581 agacccgcat tttggcaatt aaatccattt agatactata atacacaata cagctatgca
     8641 gaaggcaacc catatttaca accacaatat agctataata catcgttaga atatgtgctc
     8701 aaacaaaatt attctttctc agtatattac aactatactt taaataattc aactcaaatt
     8761 caatttacag atacttcaaa caatactaat ttctggcaat gggaagccaa aggcagacag
     8821 atgcatgatt taggtacatt taaccaattt tatttttcaa ttaaagaaaa atggcagcca
     8881 acaattgcaa tagatgtatt tatagatttt ttaaaatcgc cttatttgta taataacact
     8941 aaacgtactt cttggggaat ggcattaacg attaataatg attttaattt tgataagaac
     9001 ggcagattaa ctggaagttt aaattattat tttacacctc cagcacccag acaagaactg
     9061 tttatgcgga tgtataataa gatagatata ggtattaaag ctaagctttt aaaagaaaag
     9121 cttttatcac tttcgttttt tgcaaataat attttaagat ccaaacagag taatggatat
     9181 tttataaata caactggaca atattcatca tttacaaatt attataatga tagagttttt
     9241 actttctcta tcacctataa atttggcaat aaaaatttaa agacaataaa caaacaaact
     9301 gacaatgaaa atattagcag agcaaaataa aatacttaat cgcgataagt agtagccaat
     9361 actgtgagtg cgcaattgcg gtagcaacta caaagcttcg ttctgtataa gaagtaaaat
     9421 aacgcagcgt tctacaacga gaggattgaa tttaaataat attaaaaatg tactaaaatg
     9481 aaaaaaaatc aaaagaaaat agacctttca aattttgaaa aactgtcaga ggcatccaca
     9541 ggttcattag tgggaggttt ttcagtagct aagtctggac ctagtacgaa cttaaaagcc
     9601 gcagttaatt taatcaaatg cacaacaacg aataattgtg acggcggtaa ctgcattagt
     9661 ggatgtggtg gataatttat tgataattaa tgtcagccat tgtttaaatg gctgacaact
     9721 tatttaaaaa atatatttct tatgaaaaat aaactaaatt ttgcaagatt aaacaatagt
     9781 gaggcagccc aattaatcgg tggattttct tctgtcttct ctggaaattt ctcatgtgga
     9841 gatacttcaa aaatcaacaa ttgttatggt ggcaacttag ttgttggctg tggtaaaggt
     9901 tttaaatcaa aaaaatctag taagcatata ccgaatacaa actgtaaggg aaattgtgta
     9961 aaaggttgtg gagaaaaaac aaaatagaaa agtatgaaac ttagccaatt taacagcatt
    10021 ataccatata atgataatta tgcactttat aatagttata gtaacaaggt aataatcata
    10081 gaaaaggagt tgaaagactt attggacgca gcaaggagtg aaaatattga agaattaata
    10141 aatatacatc ctacattcta caattatttg atagaaactg aatttgtggt tagtgatagc
    10201 ataaatgagg ttgagaacgt aaagattatt agtaaaaaaa tagatgagaa taagaatgca
    10261 ttttttttaa caattaaccc cacaatgaat tgtaatttta aatgttggta ttgctatgaa
    10321 acccatatta aagactctag atttactagt gaaatgataa ttaagattaa taagttcatc
    10381 tcaaatacag taagtgacag tcaaattgaa caatttacat tgtctttttt tggaggagag
    10441 ccgttattat tttttaaaaa agatgtaatt ccagttataa gtcattttac agaaatatgc
    10501 aataaacaca atataagtaa taatataagt tttacaacaa atggttttct aataaacgaa
    10561 gaaataataa attacctaaa agagctaaat acaacttgtt cttttcaaat taccttagat
    10621 ggttttaatg aaaaacatga tttagtgaga tttgtaaata ataaaagagg ttcttataat
    10681 acaattatta ataacattaa actactaata aacaactgtt tttttgtacg actaagagta
    10741 aattatacaa atgaaaatat atctgatgct tacaagattg cagatgaatt taatgataca
    10801 gaaagcaatc tgaaagaaag ttatctactt tttgattatc atcgggtgtg gcaagatgat
    10861 aaaattgatg atattaatat tcaattagat tataataaaa agtatataag tagtaaaaat
    10921 ataaatatat tgggtaatta tagtcctgat aacgtagttt cttcttgtta tgcagacaaa
    10981 cggaatagcg tagttataaa ttataatgga gatatctaca aatgcacagc aagagatttt
    11041 acacttgcaa acagagcagg ttatttgtct aataaagggg aattaatttg ggaaaataat
    11101 tcacttgaaa aaaggatgaa ctctaagttt aataacaaac catgtttatc ttgcagatta
    11161 ttaccgattt gtaatggagg ctgcactcaa catgcaatag aacatctaaa taaggatgaa
    11221 tattgcatct attttggtga tgaagctgaa aaagataaag taattaaatc aaaagttgat
    11281 gatgttctac aatacattga aatttaatag aaataaattt gttcatcaac ttgaaagtac
    11341 tgattgtggt cctgcttgta tagtaatggt tgcataccac tttgggggta agtatacatt
    11401 aacacaaatt aaagatatat gtgagattac taggatgggt gtttctgttc aagatattat
    11461 tgatggggca aagaaaataa actttaatac tacagcatta aaactaacta ttgaagattt
    11521 ggaacaaatt ccattgccaa gtatactata ttggaaacaa gaacatttta ttgtattaag
    11581 acagatcaaa aataacaatg tttatattat tgacgatccc gcatatggaa gagttaaaat
    11641 gagtcaaaag gattttgttt cagagtggat tggcaataat aattgcaaag gaattgccat
    11701 tgtgttagaa cctaatggag aactgacaaa aattgatact aataagccaa atagagtttc
    11761 tgaatcatta aaattagtct tcaattttat aaacaagaat aagataaaat atacaattgc
    11821 tattttatta ctttttgtag ctttagcatg taactgggcg atcccaatat tctttcagaa
    11881 aacgattgat aatggtattg taaataaatc tatacaaatt atcacatatc tcttattggc
    11941 acaattcgtc ttgtttatta gtcatattat atcagataca ttaagtcata tattgttggt
    12001 aaaagtaaat tttcatttaa gcatcttatt acaaaatagc tttctgagta aattgattaa
    12061 gctacccatt aattactttg atactaaatt gaatacagat acactccaaa gaatctctga
    12121 tcaacatata attcaggaat ttactacttg gaaaggcatt gaattattta taaatacgct
    12181 aaacattata atatttggtg gtatgctctt gtctattaat tatactatat ttaatctatt
    12241 catattttta tctttaattt ctatattctg gacattttta tttttaaaga gtagagcaaa
    12301 cctcgaatat tcaattttta ttcaacaatc attgagtaat aataacatgt atgaatttat
    12361 tatgaatatg ccagaaatta aaataaatgg agctcaaaat aatttaatta ataaaataaa
    12421 aacgattcaa caaaaactaa ataatctgga acttaaatct ctttctttaa atacttatca
    12481 agtattaggt actagtttta ttaataaagc attagaaatt attgctattg caataagcgc
    12541 atattttata atagaaagta gacaaacttt ggggcaatta ttaagtatta gttatatact
    12601 tggacaacta tcatccccaa ttagaaatat tataaatttt acaaaagact tacaagatgc
    12661 tttaatttct aaaaatagac ttgatgaagt ttacaatgaa cagaatgaag ataacgaaaa
    12721 taagattatt aactttaatc atcattctaa tttgattatg cgagatgtgt catttaagta
    12781 ccctggtagt tttaactcat ttgtattaaa aaatattgat ctaataatac caaaaaacaa
    12841 gaaaaccgct attgtaggtg ttagtggaag cggaaagaca acgctaataa agttattatt
    12901 aaaatattat gacatccaaa acggcgaact attattaggt tctgaaaatg taaatttatt
    12961 gaatgcagag aaatggagag aactttgcgg aatagtatta caagacggtt ctatctttag
    13021 tggaacaata attgaaaaca ttgcattctc tgatgacacg gtaaatattg ataaaataaa
    13081 aaaagctact aaaactgcat gtatagataa atttatagaa tcattgcctt tgcaatacaa
    13141 tacgaagatt ggtaatatcg gcattcaact tagtggtggt caaaaacaac gaatcttaat
    13201 tgctcgtgct atttacaaaa atcctgattt tttatttttt gatgaagcca cttctgcatt
    13261 agatgcaaaa aacgaaaaag aaattacaga caatttagaa caatttttca aaaataaaac
    13321 agtagtaatt gtggcgcatc gtttaagcac cgtaaaaaat gctgatcaga ttgtcgtatt
    13381 agatggtggc gaaattgtag agacaggcac tcatgaacaa ttggtcaaaa acaaagcaca
    13441 ttattttaat ttagttagca accagttaga attaggaaac taataacctg agttcgggtt
    13501 aagaaaaata agctgataaa atagttgatt tcccattttt tcctccacat taataaggtg
    13561 gaggaattaa aaggaggcgg ttctacataa aaaatcagct attttttatt gaaacgctta
    13621 tcccgaactc gtgttaatta atcaaacacc caatacttgc tttgaaaacc ccagcaattt
    13681 ttcttcttgc catcttcctg ccataaattg tatgccgatt ggtaagccat tggctgtttg
    13741 atgtagtggc aaggaaatgg ccggaatgcc cgctatattg gcttgcaccg tgaaaatatc
    13801 tcccaaaaag gaagcgacag ggtcagatac ttctcctatc ttataagcag ctgtgggtgc
    13861 caccggcgac aagataaaat caaattcttc caatgttttg taggttgctt ctgctacaat
    13921 tctgcgtact ttttgtgctt tggagtagta cgcatcgtaa tagcccgcac tcaatacaaa
    13981 ggtgccgcac ataatgcggc gctgcacttc ttttccaaag ccttgtgagc ggctaagtat
    14041 ataagtttct tctaaagagg tggcttcttt ggcatggtag ccatatttta ccccatcaaa
    14101 acgagataaa ttggatgatg cctctgcggt agtgagcaca taataagccg gcacgatata
    14161 gtccagttcc ggaaattcga tagcctgtat ttcgtgtcct tccgctttta aattttctat
    14221 ggctttcaaa caagcatccc gaacttctgt atctatattc tcatgctcta ccgcattttt
    14281 gtaataggcg attttatatt ttttgtgctc gtgtaaatgt ttgctgtatg tcggcactgc
    14341 ctttggcgaa caagtggcat catattcatc agctccactg attatttcca acaataaagc
    14401 tgcatcttct actgtgtttg tcaacggacc aatttgatca aaagaagaac cataagcaat
    14461 taagccataa cgagaaacac gaccataagt aggttttaga ccaacaacac cacaaaaact
    14521 tgcaggttgt cgcacagagc ctcccgtgtc agaacctaaa gccgcaaagc ataagtcggc
    14581 agcaacggca gcggctgacc cgccggatga cccgccggaa acccgggtgg tatcatgcgg
    14641 attttttacc gcaccaaaaa aagaattttc attgctaccc cccatggcaa attcatcgca
    14701 gttagttctg cctatgataa tggcatcttc tttcaataac cgctcaataa cagtagctga
    14761 atatggagaa atgaaatttt cgagtatttt ggagctggca gtgcaaagat gattattgta
    14821 aacgatattg tctttaatgg caatgacaca accaaacaat ttacccaaag gcaaaccttg
    14881 cgcaattttg ttgtcgatgt tttgtgcgga ggttaatgca gttgtttcaa aaatttcgtt
    14941 gaaagcatta aggtgagatt gttccttaat tttatggata taattttggg tgatttcagt
    15001 acaagttagt gtcttgttca ataaatcctg ttgcagcgca gcaagtgttc tgtacgaatg
    15061 agccatcagg aaaatcagaa attattgtgc tgttttatca gaagatttta catcttcatc
    15121 tttcatgcct ttttcaaatt catcttgcag attggctttg gcagaattaa attctttaac
    15181 gcctttcccg attccgcgca ttaattcagg aattttttta cctccaaaca acaataatat
    15241 ggcagcaaaa atcagaaaaa tttctcctcc acctaaatta agaaatgaca ataaattgta
    15301 catggttaat tatttctgca aagttagcag aattttctta aacgaaatgt taaagaatat
    15361 ggttttgggt cgatagacta ttgaccacag catatacagc taattactta ttcacctgag
    15421 atcgggttaa ggaaattaag gtgaaaaaaa atacttgatt tctcctattt tcctccaact
    15481 taacaaggag gaggaattaa aaggtggtgg ttctatatca aaaatcaact atttttaatt
    15541 taaacgctta tcccgaactc aggttattcc atctacttaa aactatcgaa ccggacagct
    15601 ttactgtcct ttgaacttgt caatatccct gcgttctttt tttgtaggcc tgcctacgcc
    15661 tttatcccgc acttcataaa agaatgcagg aaattttagt ttatctattt cttccagtgg
    15721 ggttaagtct tcataacatt gttgtgcaat tcccgcactc actcttttct ctatgatttt
    15781 cagtacttta tacttatatc tcactccttg tttatttagt tccatcagca tcccgacttt
    15841 tatctggaag gcggatttca ctttttcatc atttactttt acttttcctg cttcacaagc
    15901 ttccgaagca agcgttctgg ttttgtataa acgaacggca tgcagccatt tatctattct
    15961 tgttttttca gcttccattt ttttggcaac aaatttgata tttagaatta caaatgcaat
    16021 ttcataattt tgaggagaat ttcgctatga gaaaaataat actttttggc atcattatta
    16081 tcgctttggg tgcttttgca aaaaaaccac ctaaaaaaac aaaaacacct acggcaagta
    16141 cttgggtgga accggttaaa ccaaaactga tatacggcaa ctggacttgg tttgagacgg
    16201 actgttgcgg ttccagacgt ggcgtatcta caccggaaag ttcaggggat aatattagcc
    16261 ttgaactcag aaacgattat acatttcttg aaacgcacag caaaaaaaat gcacttccca
    16321 gagaaggtaa tattatttta tttaagcaag acggatataa tatgattcag tttaatgatg
    16381 aacgcccggc gagatataca ctttctgaca ataatgatac actcacgtta agttggaaat
    16441 atatggaact gcaaacggaa aaatatatcc gtaataaaaa ctaattacgg caataatatg
    16501 cccaaaatac ttgcactcga ttatggtaaa aaacggactg gcttagcagt gtctgacgaa
    16561 agtaaaatat ttgctttcgg gtatggtacg caagacacca attcactgat gatttatttg
    16621 gatggattgc ttcagaaaga gaggatagat actttgcttg ttggcatgcc gaaaaagctg
    16681 aataatgaag atgcagaaat aagtacagca attagaaaat ttattcaatt atttaaagag
    16741 aaacatacag ctattatagt ggtagaatgg gatgaacgct tcacctcaaa aatggccatg
    16801 cagagtatgg tggaaagtgg catgaagaaa aaagacagac aaaacaaagc cactatagat
    16861 gaggtgagtg caacaatttt actgcaaagt tatcttgaaa cgctgtaact tccattaatt
    16921 ttaaagctaa ctttctggat gttcagccag aggatcttta gtaacaattg cccaatatac
    16981 ccacatggca aaagtaatga gtaaggtaat ggtcggttga aaaaatgcta cgcaagcagc
    17041 aagaaagtat aagcttattg ccagcagttg accacgcttc aggttgatta gccattttat
    17101 atttttatca gcatcaataa tatattgagg agttcgcaat atgcctaagt ttagacaaag
    17161 catactgaaa acgataagcc cggtgaatgc agcataaaat gcaacggcag tgttaactgc
    17221 aggagtgcca ataaaaatac ccacggtgcg agtaggataa ggaaagagaa ccacaaatag
    17281 caacacaaga ccatttaaca gcaaaacgga atgtgatgct ttacggatac gattgaacaa
    17341 ttgatgatgt cccatccaaa ttaacaaaac cgtggcgaag gaattaaaat aagcaaaata
    17401 ctctggtcat ttctccacta ttttttcaaa gaggtttcta ttggtgggat cttggtattc
    17461 agatatacct atttcaattg ccagcaaggt aatagcaacg ccaaatattc cgtcactaaa
    17521 tgcctcaatt cttccggttt ctttttccat atttatttcg ttgaaagggc aggatattga
    17581 gaaagataag tcggttgttc cgcttgtttt accatgaaat cagccacatc atccctgctg
    17641 atactgccta tattaatgcc tttagacaat tttgtttctg tgcgataatt ttctgtcaat
    17701 ggtttatcgg ttagtaagcc agggcgtact atttcccatt tcaaatcaga tttagcaatc
    17761 agctcttcca tgatggtttt atcggcatat acagatttta acaacaagct gaaaaatatt
    17821 ttcataaaca gcgcattgta tgcgccactg tcacctgcac caaaaccagt caaacaaata
    17881 aaaggctgcc ggtatttatt ttcagaatga agttccaaca atagtttcgc aaaatcagaa
    17941 tatagtgtcg tagctttcat gcttttacca gtaccaagcg caataattac tgcatctgca
    18001 ttttgtatag ctttgcttaa atcggattta ttagtggcac ttccggatat ttgtattaaa
    18061 ttttctgatg ccggaagttg aatcggagaa cgagataaag tagttacttt gtgatttctt
    18121 tgcaatgcac gttttacggt tgctaatcca acacctgcag atgcgccaat tacggtgata
    18181 ttcattgtat attaatttgt tatttaaaat tcttccacat catgctctcc atgggtttag
    18241 gtgagcgttg gttatatttg gcagattgct ttttattata agcatgttcg atatctcctt
    18301 tcacttcaaa atatataagc tgtcctacgg gcatacccgc atacacacga actggctgaa
    18361 tgcaagatat ttctaaagtc cagtggttgc aaaaacccac atctcctttt ccggcagtgg
    18421 catgtatatc tatacccagt ctgcctatgc tcgacttgcc ttccagaaag ggaacatgtg
    18481 catgcgtttc cgtgtattct tcggttacac ctaaatagag cactccgggt tgtagaacaa
    18541 agccatcctc accaatttca aattcctgaa tcgggttgtg tttttttgca tcaattactc
    18601 tgtcggtata tacggcgaga tattttccca aatgtacatc atacgaattt gtacccaaat
    18661 aggctctgtc atacggttcg atgacgatag tccctttctg catttcatcc aaaatgcctt
    18721 tgtccgttaa aatcatggcg caaaagtacg attataatct cagacctttt atattgcaga
    18781 ctttgtcttg gaataaaaat gctgctcgtt actatactaa acacaaatgt gaacaaggat
    18841 gaggaaatac tctgctgata ggttgagttc tattactcta gctcacagcg ttcgctgtca
    18901 ttggcttcct atgaactgtc atgaaagaca agatttaaac accaattagt caaagataat
    18961 tgtgtcgcca ctacgtggct tgttttttaa cgaattattt accattaggc attaaggaaa
    19021 ttaagaattt ttatgcgaac aatgccacta cgtggcttct tgataagact aaatttgcac
    19081 cactactaaa ctattaaact cattaactaa tccaccaatg aaaggtgctg ccattacatg
    19141 gctcagtttt aggttgtatg tcttatatat aaatgttttg ccaatacgtg gtttgtatct
    19201 taacgaatta tttaccatta aggcattaag gaaattaaga atttttatgc gaacaatgcc
    19261 actacgtggc ttcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngataa
    19381 gactaaattt gtaccgctac caaactattt agctactaaa ctattaaact aattaactaa
    19441 tcagatgcgc cacttcgtga cattttaaaa aatagtatgt ttccaaaaaa aggaaaacag
    19501 ctaaatttag cactccacaa tttaccttct tggaaaggtc agataacttt tcacttcaaa
    19561 ttttcccgac gcacttgagt tttccggttg caatatgaaa tttaattatc aagcctataa
    19621 gctttgaaat tatatcactc gttcaaaata ctgaacaata ttaagactga taaacaaaat
    19681 ttcaaaacta taattttttt accctgcaat ctctacgcat aagctattct tccatagaac
    19741 aactctttct tattccttat ctttgttaca tgtccgcaaa tttgcagagc cgcattcccg
    19801 ttatggagca tttttatacg cttcagggcg aggggtttta tgccggcacg gctgcttact
    19861 ttgttcgttt agcaggttgt gatgtaggat gtcattggtg tgatgtaaaa gaatcctgga
    19921 tagtggatga aaagcaatgg atgaatatca gcgacttggt agatgacatt aaaaagacgg
    19981 ctacgcagcg tgtagtcatt accggaggcg aacctttgat gtacgacctt ctgccactga
    20041 ctaccgcctt aaaaaatgaa aactatttta tccatattga aacatcaggt gcttacccgc
    20101 tttccggaat tttagattgg atttgcgttt cacccaaaaa atttaagcct gttttaaaag
    20161 aagtattact ggccgcaaac gagctgaaat gtattatctt tcacaaaagt gattttgcct
    20221 ttgctgaaca gaacagagta ctcacaaatg agaagtgtca gctttatctg caagctgaat
    20281 ggtcgaagcg tgagttgttt tatcccgaaa ttgtagccta ttgtaaacaa tatccgtatt
    20341 ggaagttgtc ggtgcagaca cataaatatt tagacattcc ctaaactaca attgtataaa
    20401 aagcgttata tggtattggc attctctttg atacaattac taagatgaaa aaaacgattg
    20461 cttttttatt cgtgttctta tcggcttacg ggctatttgc ccagcaagta gctgaaaaag
    20521 caatgtctgc ttatttcaaa gcaaaaggct atgcacaatt tacgcaatgg gaagaaggaa
    20581 ttgccgagtt gaataaagcc attaaaattc aaccagattt cctggatgct cacgccttgt
    20641 taggcgaaat gtattatgcc tccaaaaaat atgacagttc cattatcgta ttgcaaaaca
    20701 ttatagaaca acccggtatt ccggctcgct catattattg tctttctgaa tcctatctca
    20761 aaaagagtga aattgcaaaa gcaaagcaat atgcccagca atacctcaat caacccaacg
    20821 tgcaggagca ttttgcccgc aaagctcaac gtactgttat caatggcaat tttgcagaag
    20881 aagccatgaa acatcccgtg ccttttaacc ccaaaaatcc gggagctaat attaatacaa
    20941 agtcacctga atacttccct tacttgactc ccgatggcag ttattttttg ttcaccagat
    21001 tagagaataa tcaggaagat gtttattatg ccaaaaagac ggataccagc ttcagccctg
    21061 cacaatcatt tggcaccaat ataaatactt ttgaaaatga agcagctgcg gtgatgaatg
    21121 tgagcgggaa tttgttattt ctgaccgagt gtaataaact tgatggattt ggaagttgtg
    21181 atatttattt ttctcaaaac aacaacggca actggtcaaa acctatggga attggcaagc
    21241 cggtaaacag cggggcttgg gaagcacaac cctcatttag ttcagattca aaggctcttt
    21301 atttttcgag caacagaaaa ggcggctttg gcggacgaga tatctgggtt acttatttag
    21361 acagtgcgat gcatttcagt gaacccgtaa acttgggcaa taaaattaat acgccttatg
    21421 acgaccagtg cccatttatc ttcagtgata acaaaacgct ttattttact tcagatggct
    21481 ggccgggtat gggtgccgga gatattttta tggcacaact taccgacaca ggctggacaa
    21541 atcccaaaaa tttaggctat cccatcaaca ctgagagcga tgacaacggc cttaccgttt
    21601 cgtatgatgg caagaccgca tatttatcct ctaaccgcag caacggatat ggtggcttgg
    21661 atatttacag ctttgaattg ccggatgctg tccgccctcc aagcggaact tttgttaaag
    21721 ccattattat ggataaaaaa acaaaacaat tattgccagc aaattaccgg ttgattaatc
    21781 ttgaaaacaa gagcgaaata ataaatggta ttgctaaaaa tggcagtttc tttgaaagaa
    21841 ttaatgccaa taagaattat gccgtgcaag tggaaaaaga cggctatctg ttttattcgc
    21901 aaaatataaa tctcaaagcg aatactcagg taaaaccttg ccagctaaaa attgaattag
    21961 aaccgattgc agtaaatgca aaaattacgc tggataatat tttttttgat tttgataaat
    22021 ttgatttaaa gtccgaatct tttgctgact tggaaaatct gaccgggatt ttgaaaaaga
    22081 atccagggct aaagatcgaa atcagtgggc ataccgacca caaaggtgat gcaaaataca
    22141 acctcaactt atctcaaaag cgagcagaaa gtgtattaaa ctacttagta caacaaggaa
    22201 tttcggcttc cagattaacg gctaaaggct atggagatac ccagccagtt gcccctaacg
    22261 atacggaaga aaaccgctcg cgcaatcgcc gtatcgagct aaaagtaata caaataaatt
    22321 aagtgtttaa agctggctta gaaacgttcc agtttgctaa agaagaaatc gccttctatt
    22381 tgtgcatttt cgttagaatc agaaccgtgt actgcatttt cgcctacaga ttttgcgtat
    22441 actttgcgga tagtaccctc ttccgcattt gcaggattag tagcaccaat taa
//
LOCUS       DAOQII010000006        21578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1098_length_21578_cov_4.658040, whole genome shotgun sequence.
ACCESSION   DAOQII010000006 DAOQII010000000
VERSION     DAOQII010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21578
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1098_length_21578_cov_4.658040"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /gene="mutS"
                     /locus_tag="PLF48_00670"
     CDS             <1..1377
                     /gene="mutS"
                     /locus_tag="PLF48_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNF47860.1"
                     /translation="TIVEDAPAVLQKGNVIKHGVNNELDELRKLSSSGKNYLLEIQTR
                     EADRTQIPSLKIGFNNVFGYYLEVRNTHKDKVPNDWIRKQTLTGAERYITPELKEYEE
                     KILGAEEKIQTLEIQLFENLIKELMDYIQPVQLNAQLIARLDCLQSFAVVALKNNYRR
                     PQFTEDTALRIEEGRHPVIEQQLPLGEQYIPNDVYLNPEDQQIIIITGPNMSGKSAIL
                     RQTALITLMAQTGSFVPAKKATLGIVDKIFTRVGASDNLSLGESTFMVEMIETASIVN
                     NISDRSLVLLDEIGRGTSTYDGVSIAWALVEFLHTNKTAHPKVMFATHYHELNELAEK
                     FNRIRNYNVSVKEAGNKVIFLRKLIEGGSAHSFGIHVAKMAGMPNEVIQRANEILFEL
                     ESNRNLATDIQSTLKHIPAKQDFQLNMFAMDDPRLLKLKEVLEKVDINTLTPVEALMK
                     LDELRKLL"
     gene            complement(1383..1838)
                     /locus_tag="PLF48_00675"
     CDS             complement(1383..1838)
                     /locus_tag="PLF48_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47861.1"
                     /translation="MADISNNTPSNQNQTNPALDVKKKPSAIKRFFRKFLLFLGLFML
                     VLFLGIWAYFSFPYSKGNRAGSLIKFSEKGYIFKTYEGEMNLGGVNPMPGNTIVNNIW
                     TFSVKDKTVADDLMEMEGKEVRLHYIQYLGKLPWRGETEYMVDRVEEVQ"
     gene            1889..2551
                     /locus_tag="PLF48_00680"
     CDS             1889..2551
                     /locus_tag="PLF48_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF47862.1"
                     /translation="MAKSSTHEFRETYRNTIIGKKYNGKLHFWGITLFCIVIVSFAVF
                     RIQSPNWKEILVIPVSFILVNAAEYFGHKGPMHHLNKRLAAVFKRHTLQHHEFFTFDD
                     MYFDSQNDAKAVLFPPILLLFFFFFFALPCWVLFYLLWSANAGYIFIATVFVYYLNYE
                     WLHYAYHLPPEHFISKLPFIKILRRLHHVHHEKSLMTKYNFNITYPIFDWLFGTYKKN
                     IQ"
     gene            2804..3172
                     /locus_tag="PLF48_00685"
     CDS             2804..3172
                     /locus_tag="PLF48_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47863.1"
                     /translation="MPKLQLATAIGIILFWIAFFTIGFESPAYPSYYTKFEHSFPLPD
                     AFLSLMLILAYLKRNTKSWKKYTLIAAGAMVFLGLCDFSFNALNGMYQISITDTLLNA
                     FINIWCISFGLYQLVNVKLD"
     gene            3319..4788
                     /locus_tag="PLF48_00690"
     CDS             3319..4788
                     /locus_tag="PLF48_00690"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNF47864.1"
                     /translation="MKKYNPIYLLAFLFGIFTLSIFFTSCKKDKLNTTSGLQFSNDTL
                     TFDTLFTTLGSTTRFFTIRNKENKAIKISNIHLGGGTDSRYRINVDGDAGTSFTDIEI
                     PAKDSLYVFVEVTIDPNDQTLPYLVLDSVQFQTNGINQHVILQTFGQNAHFFYADSIT
                     ANTVWNNDLPYVILNYLQINQGAQLCINGGTKVYFGPGAAMVVEGDLKINGTDTNNTV
                     LFRGVRLDEDIAGRPYDDFPGQYAGLFFLRNSTGNINYLEMRNAAYGINVGNIKTTDD
                     PTVNLQTLLDMKWSNAPYVVIKNSKIYNQAYYGIFGFLGRIEAENLLVYNCGTNAVGL
                     YCGGEYDFTNCTFYTRGSAYVSHTKEPLFYINDYFTYSTSSPSILADSTRAYFNNCIL
                     YGTLEEEIVTDRETNSTHAFDLLFTNCDLKTQQTLSLPFYNTCKTDDPQFKDVYKNDY
                     HLKSGSPCIDFSNPPSTSTDIDGFSITGAKRDCGAYEYR"
     gene            5045..5614
                     /locus_tag="PLF48_00695"
     CDS             5045..5614
                     /locus_tag="PLF48_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47865.1"
                     /translation="MQYKNIPTCGRFFKKQVDTIRIEIHEQVLYANIAFWKKFLVVFM
                     LCFSTQLFAVEFILTDGLLTDSTGVFISDSSQLNPTDTELNTDTTPLLHVDTESISPE
                     IIFNTIHAGNILSGFCTIISGGVSMEIETTKVGPLKDDTIETKSSSSTTIRKSVNKNN
                     RESKPIELPHQDGMLPETALYLPNRKRVS"
     gene            5812..7395
                     /locus_tag="PLF48_00700"
     CDS             5812..7395
                     /locus_tag="PLF48_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HNF47866.1"
                     /translation="MHTILPFFLAMLAVIVLLNMWANKLKIAYPILLVLAGLLISFIP
                     GLPMLNINPELIFFIFLPPLLFEAAWSVSFKEMKKWYRIILSFAFLVVFFTAFAVAVV
                     SNHFIPGFSIALGFLLGGIVSPPDAVSTAAITKFVKIPKATSAILEGESLLNDASSLI
                     IFRFALVAVGTGQFIWQEAAVSFVWMVVGGAAIGLLAAWFFMQAHKRLPTDAPSDIVL
                     TFIEPYFMYWVAEQLHSSGVLAVVAGGLFMSTKRLTFLNATSRIRGLSVWESFVFILN
                     GIVFLIIGLGLPEVVQGMRSNGIPLGTAIGYGILVTIVLIAARMISSYAAMIATRIFR
                     PTVFNSFANKRIRWWLPLVLGWTGMRGVVSLAAALSIPVTLADGSDFPKRNLILFVTF
                     VAILLTLLVQGLTLPYIIRKTRIFDHVFATEHEEETRKKMKHGLKKQVYTYMKEKHEN
                     KLIEYEGMELILKQWEERAKAENDNWVTDHTKALFLEILEIQREFLMELNKDPEINEE
                     IIRQQLYQLDLEEERWRVV"
     gene            7416..8069
                     /locus_tag="PLF48_00705"
     CDS             7416..8069
                     /locus_tag="PLF48_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF47867.1"
                     /translation="MKIIITGATGMVGEGVLLVCLEHPEVSEVLSVSRKPIAHQHAKL
                     KELILSDFSKLDEHEELLKNYDGCFFCAGVSSVGENEESFTRKTYDFVVPFARLLSGI
                     NNEMTFIYVSGSKTDSTEQGKVMWARVKGRTENALVKLPFKSVYNFRPGFMKPVKGQK
                     NVNAVYRVLEVFYPFWLLVLPNWTCSMREVGLAMIHCVSRGYNKHVLEVADIKVQAK"
     gene            8108..8689
                     /locus_tag="PLF48_00710"
     CDS             8108..8689
                     /locus_tag="PLF48_00710"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNF47868.1"
                     /translation="MRKERFEAISDAVLAIIATLMVLEIKVGDLTDASLKQFVFQLLV
                     YAVSFAYIAILWINHHNMLRHVEKIDMKIVWVNFWLLFFTSLIPLATSTINEEFYNTR
                     SHLFFGSVLTVVVLFYSILEELVYKITKHKYNESNRKMNWLSMCVFALSIPLSYVSIY
                     ISSAIFILIPFSYFLLPKKANQHFSNLPGNLNR"
     gene            8778..9710
                     /locus_tag="PLF48_00715"
     CDS             8778..9710
                     /locus_tag="PLF48_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF47869.1"
                     /translation="MQSLQSLTVKTIINLVRSSLFVHKTDALTVRVGFERVSNLMKFP
                     KFVARKETKVAGIPACWFTPDGHGKSKVILYFPGGGYVCGSYNTHRALIARIARSSGY
                     KVLAVDYRKAPEDPFPAATDDALAAYKQLLRDGYENIVLMGDSAGGGLVLALLQLIRK
                     HKLNPAIGSVLLSPWTDLTLSGESMHTKKDIDPFIKPNLVEIFARRYCGENSPRNPLI
                     SPHFADVSGFPPTLIHVGSHETLLDDSVRMAQKMSEANVDVKLSVYKNMIHVWHFLGG
                     IMPEANQAIQEIGEFVKSIKVNKKTAHLDALAVY"
     gene            9779..10732
                     /gene="fmt"
                     /locus_tag="PLF48_00720"
     CDS             9779..10732
                     /gene="fmt"
                     /locus_tag="PLF48_00720"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNF47870.1"
                     /translation="MKKDVRIVFMGTPEFAVASLDALCLNGFPVVAVVTAPDKPAGRG
                     NLMHQSAVKKYALEKQIPVLQPEKLRAEDFLNQLRELHANLFVVVAFRMLPEIVWSMP
                     ELGTFNLHASLLPQYRGAAPINWAVINGEKETGVTTFFLQQEIDTGNIILQEKIKIED
                     TDNAGTVHDKLMILGADLVVKTANLIANQAVTQLPQNEAGLSGHELKHAPKIFKDTCK
                     IDWNQKAEQIHNLVRGLSPYPAAFTFLDNKSVKIYASAIRHAGAEEMQRFHLTANIYT
                     DKKTFLSFSCADLFLDILEIQLEGKKKMKIDEFLRGHKFSQ"
     gene            10870..11601
                     /locus_tag="PLF48_00725"
     CDS             10870..11601
                     /locus_tag="PLF48_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47871.1"
                     /translation="MPTLKIIIQVFFQIAIFSIYSLSRSQTISNQIERFGELQIDLDS
                     ISDYNIVRLDSTQDIKYLSITATNNSLSQDSINLSPFKNLVYLKIDGYKLINLKRLFL
                     MLKNNKKLQVLILNNNCIESIPSNIQSLKNLTFLDLNWNSISSLPRNLKKLSSLNTLI
                     LGSYKTTHSPSKLSNKFQDVPKVVLKLKKLNTFIITNNKLQKINKKMIDKLKKIKHLG
                     ILNGNYFEDPNSIKSELEKYNIEIW"
     gene            11750..12463
                     /locus_tag="PLF48_00730"
     CDS             11750..12463
                     /locus_tag="PLF48_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47872.1"
                     /translation="MKRIFLYIIFIIQPLSFCASDLIFASYNNGTICFENNIAIVKLD
                     IRKNKYFYSIENGTQKRSLSYGYFNFLNDSIIQITSDFLDTTFVNSINYYIENKNRFF
                     FYDNHDKSKLKIKGNKLLIYDIDTHVDFLDTMSTNDSLLNYFVNHYPIYPDSYFYKYD
                     ISNDSNFIKDGNIIFPFDMGIILQNSNNYVKIFTKDFWISISSNNINRIYSNIVMKNH
                     IISNTIKGNSIFIYFSKNK"
     gene            12538..12885
                     /locus_tag="PLF48_00735"
     CDS             12538..12885
                     /locus_tag="PLF48_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47873.1"
                     /translation="MLLKIFKISLIVFVSIVFIIIVSFQVKAELYMHKQDNTYGILKN
                     KQRKSFLPFVWFYFINKNKDKTITGKQSLEFYFKDAGIRGVVVCSYIYDAYMYHVYSG
                     SWHLPDSITTSIQ"
     gene            13374..14729
                     /gene="mgtE"
                     /locus_tag="PLF48_00740"
     CDS             13374..14729
                     /gene="mgtE"
                     /locus_tag="PLF48_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNF47874.1"
                     /translation="MDDDSDLKNRILLALSGDAVLLEDFNTSDFVDFIEEEPSYAGQI
                     IASLEPHRAVKTLKFLDIKLQKKLIHSLQPEVVATLIQQMHFDDRTALLSELPPEVVK
                     QLVLHLSHDDRMETLSLLGYPEDSVGRLMNPDYIEVQVTDTVEDALSAIRSYGREIEN
                     INFIYVVDKQEKFLDDINIKELLIVDLSTKISTLMDSKFISLNVNDDREYAASMFKRS
                     NRAVLPVVDNSGVLMGIVTIDDILRVESEETTEDIHKLGGTKALDGAYISASLSNLIK
                     SRLPWLLVLYLGEMLTIHVMNYFNIEIKESVIIAFFIPLLISSGGNTGSQASFLIIRD
                     LALDEISFDDWFKVFLKELASGLILGLVLAIVGVGSILIWQNIYPIFGENYILLSLTV
                     GLSTLCVVLWGTLCGSMLPLLLKKIGLDPTTSSAPFIATIVDVTGLIIYFTIAYNLLI
                     H"
     gene            14922..15896
                     /locus_tag="PLF48_00745"
     CDS             14922..15896
                     /locus_tag="PLF48_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNF47875.1"
                     /translation="MKYTHIFMLALTFLFVACNTTSKKDENQNAQKGIAINIDGSSTV
                     YPITEAVAEEFKKQFPDIKVSVGVSGTGGGFKKFGRGETDMNDASRPIKPEEDSLAKA
                     NNIAYEHFLIAYDGLVVVVNPQNTWCKDITVAELKKIWEPAAQGKIMKWNQIRAEWPN
                     EEIHLFGPGVESGTYDFFTEAIVGKSHSSRGDYTASEDDNVLVQGIASDKDALGFFGM
                     AYYEENTDKLKLVGVDDQKEDNGKGAILPTVETVKNKSYAPLGRPLFIYVNDIAAEKK
                     EVQDFINFYLNNMVKLSSEVGYVPLTDEEANAEKSKWMEFLKQHGGIK"
     gene            15914..16789
                     /gene="pstC"
                     /locus_tag="PLF48_00750"
     CDS             15914..16789
                     /gene="pstC"
                     /locus_tag="PLF48_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNF47876.1"
                     /translation="MKLREKIIESLLFLSALVSVLTTIAILWVLLSESSAFFKQVPVW
                     KFLTDSQWTPLFADKHYGIMPLVAGSFLTTVIAIMVALPVGLSIAVYLNEYASKKIRN
                     TLKPVLEILAAVPTVVYGYFALMIITPYLQKIIPGLSGFNALSPGIVMGIMIIPLVSS
                     LSEDALNAVPNSLREASYGMGATRFQTAFKVLIPAASSGIIVSVILAVSRALGETMIV
                     AIAAGQQPRLTLNPLVPVETITTYIVQVSMGDVVQGSLEYKTIFAAGIALFVFTFLLN
                     NMSFWVKKRFQEKYE"
     gene            16790..17650
                     /gene="pstA"
                     /locus_tag="PLF48_00755"
     CDS             16790..17650
                     /gene="pstA"
                     /locus_tag="PLF48_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNF47877.1"
                     /translation="MKSIAQKNRIKDTLFKYFGIFSTCFGLIMLMIFITNILIVGLPR
                     LNLQFFTSFPSRKAEEAGILVALVGSLWIMMLTAIMAIPLGIASGIYLEEYGKKNKFA
                     QFIEINITNLAGVPSIIYGLLALEIFVRILGLGKSILAGSLTLALLILPIIIVATREA
                     LKAVPHSIREASFALGATKWQTIWHQVLPASSGGILTGVILALSRAIGETAPLIVVGA
                     VLFVTTLPMSPMDDFSILPMQVYNWLSRPQKAFTVNATAAIIVLLFITFMLNGIAIFL
                     RNKWQKKVKW"
     gene            17692..18441
                     /gene="pstB"
                     /locus_tag="PLF48_00760"
     CDS             17692..18441
                     /gene="pstB"
                     /locus_tag="PLF48_00760"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNF47878.1"
                     /translation="MKAITEDINVYYGNHHVIKNTSLDIAANTVTAFIGPSGCGKSTF
                     LRLLNRMNDYLSEFRMKGRVLIDGVNIYDKQVHVEELRKQVGMVFQKPNPFPKSIYEN
                     VVYGLRIQGINDKSKLDETVEKSLRHAALWDEVKDNLEKSALALSGGQQQRLCIARTL
                     AVQPTIILMDEPASALDPISTAKIEELIYELKNEYTIIIVTHNMQQASRVSDKTAFFY
                     LGDLVEYSSTKKMFLNPNKELTQNYITGRFG"
     gene            18456..19172
                     /gene="phoU"
                     /locus_tag="PLF48_00765"
     CDS             18456..19172
                     /gene="phoU"
                     /locus_tag="PLF48_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNF47879.1"
                     /translation="MNYLEQEIYQLKISTLELWDLVLSQLNKSKEALQHYDKNLAREI
                     KATEKRVNALELKLDRDCENIIALYNPVAVDLRFVIAVLKINMNLERSGDMAEGIAKY
                     ILKSESQFASTLMEQARIIYMLDEAIVMLEMVRMAFEQEDAKLARQVFEKDEILNSIN
                     KSIEQVIGAYIIEYQQDIPNALHILSSIRKIERVGDQSKNIAEEVIFYLEAKVLKHMK
                     KRELRTENQQDIEGQSKGND"
     gene            complement(19155..21224)
                     /locus_tag="PLF48_00770"
     CDS             complement(19155..21224)
                     /locus_tag="PLF48_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNF47880.1"
                     /translation="MKHKLLQMPLIKTVLPLIAGIFVFDLLNVSVLVLSSISLLLFII
                     LLLLHVRVNNPGQKNRVSILFYILLFFVGGAIISAKHLSQQKNHFSSYTSGIATAYIT
                     QPIKETEKSYKTVFAISELVDSQFNVHPVHGKLLVYFAKDSSLLHLSIGDKLIFPLKF
                     QPIQEPKNPYQFDYKNYLAHKSIFQQQYLQADQLKVTGKKMRYAVRRYAYQLSIYAQK
                     ILTKYIPEKSHLAFAKGILLGISDDIDDELYNEFAYTGILHILSVSGLHVGIIYGILM
                     FFLSLIKDKSKKTRITKAIFVIAFIWTFAFVTGLSSACVRAALLFTLLTFGTENKIYF
                     NNLNLLAGSALIQLLLDANLLFDVGFQLSYLAMLGLFIFYKPFYSLLYVRHNLPDKIW
                     KLWAASLAAQVFTIPLSIYYFGNFPVYFLIANIFAIPLSAVILWLCIGLVFFSWIPFV
                     GYVLGLFLSTIMYIFIALTAYVAHLPFGKLNTLHLLPLQLVILFVAVTLFTFFIEWKK
                     PKYLMACLSLCLITLAISYFENIKQVQQSELIFYSIPKKDCIAFNENSTSTILVSDTL
                     STKDYTFNILNAQRYYDIQQTKLELLDSLQDYSNLLFFKDKTIYLLNKENQYKDFASP
                     LKIDYLYLSDNIYLNIEQVLKNYQFKSILIGSSNDYRHQHIFEKILRENKLNYYNLSE
                     KALVISL"
BASE COUNT         7120 a         3582 c         4104 g         6772 t
ORIGIN      
        1 actattgtag aagatgcacc tgccgtattg cagaaaggaa acgtcatcaa gcatggcgta
       61 aataacgagt tggatgaact gcgtaagctg tcatcaagcg gaaaaaatta tctgctggaa
      121 attcagactc gcgaagcaga ccgcacgcaa atcccaagcc tgaaaatcgg gtttaataat
      181 gtattcggct attatctgga ggtacgcaat acgcacaaag acaaagtacc caacgactgg
      241 atacgcaaac aaacgcttac aggtgcagag cgctatatta cgcctgaact gaaagaatac
      301 gaagaaaaaa tattgggagc agaagagaaa atccaaacgt tggaaattca acttttcgaa
      361 aacctgatta aagaattgat ggactatatt cagccggtgc aactgaatgc acagttaatt
      421 gcccgcttag actgtctgca atcgtttgct gtggtagcat tgaaaaataa ttacagacgt
      481 ccacaattta cagaagatac tgctttgcgc atagaagaag gcaggcatcc tgtgatagag
      541 caacaactgc ctttaggcga acaatatatt ccgaatgatg tgtatttgaa tccggaagac
      601 caacagatta tcatcatcac cggacccaat atgtcgggaa aatctgccat tttacgccaa
      661 acggctttaa taactttaat ggcacaaaca gggagtttcg tgccggcaaa aaaagcaaca
      721 ttaggcatcg tagataaaat atttacaagg gtaggggcaa gtgataattt gagtttaggc
      781 gagtcaacat ttatggtgga aatgatagaa accgccagta tcgtcaataa tatttcagac
      841 agaagtttag tattgctgga tgaaatagga cgcggtacat ctacgtatga cggtgtgtct
      901 attgcctggg ctttggtaga atttttgcat accaataaga cggcacaccc gaaggtgatg
      961 tttgccacgc actatcacga gttgaatgaa ctggcagaaa aatttaatcg catccgcaat
     1021 tataatgtaa gtgtaaaaga agccggaaat aaagtgatat ttctgcgtaa attaatcgaa
     1081 gggggcagtg cgcacagttt tggtattcat gtggcaaaaa tggcaggcat gcccaatgaa
     1141 gtcatacaac gtgctaacga aattttattt gaactggaaa gtaaccgaaa tttagcaacc
     1201 gacatacagt caactttaaa acacatcccg gcaaaacaag actttcaatt gaatatgttt
     1261 gccatggacg acccgcgctt gttaaaactc aaagaagtat tggagaaagt agacatcaat
     1321 actttaactc ctgtagaagc tctgatgaaa ttagacgagc tgagaaagtt gttgtaattt
     1381 ttttattgca cttcttctac tcgatctacc atatattcag tttcgccacg ccaaggaagt
     1441 ttgcccaaat attgtatata atgcaaccgt acttctttgc cttccatttc cattaaatca
     1501 tcagcaactg ttttgtcttt tacggaaaat gtccagatat tattcactat ggtattcccc
     1561 ggcatagggt tgacaccgcc taagttcatt tcgccttcgt aagttttaaa aatatagcct
     1621 ttttcagaaa actttatcaa tgatccagcg cgattgcctt tgctgtatgg aaagctgaag
     1681 tatgcccaga ttcctaaaaa aagaactagc ataaacaagc ccaaaaagag caagaatttt
     1741 ctgaagaatc gctttatggc agatggtttc ttttttacgt ctaatgccgg attagtctga
     1801 ttctggttgg aaggtgtatt gttgctgata tctgccatgt caaatttttt tactaaatta
     1861 actcaattta tacaactcaa caatttttat ggcaaaaagt tccacacatg agtttagaga
     1921 aacataccgt aatacaataa tcggaaaaaa gtataacggt aaactccatt tctggggaat
     1981 cactttattt tgtattgtaa ttgtatcatt tgctgttttc cggatccaat ctcccaactg
     2041 gaaagaaatt ttagtaatac ctgtttcttt tattttggtc aatgccgctg aatattttgg
     2101 acacaaaggg cctatgcatc atctgaataa aaggttggca gctgttttta aaaggcatac
     2161 cctgcaacat catgaatttt tcacgtttga cgacatgtat tttgatagtc agaatgatgc
     2221 caaagctgtt ttgtttcctc cgatattatt gttgtttttc tttttctttt ttgcccttcc
     2281 ttgttgggta ttattttatt tactttggtc ggcaaatgcc ggatatattt ttattgccac
     2341 cgtatttgta tattatttaa attacgaatg gctgcattat gcttaccatc ttcctcctga
     2401 acattttatt tccaaattgc cttttataaa aatattgcga agactacatc acgtacatca
     2461 cgaaaaatct ttgatgacaa aatataattt taatatcact tatcctattt ttgattggct
     2521 gttcggtaca tataaaaaga atatacagta aggcttttta ttaaataaaa aagaacaatt
     2581 aaaatggagt agaatattta gtgatgttct ttgattaata aactgagttc gggttaagaa
     2641 aaacaaggta aaatagttga tttcgcattt ttcctacaac ttaacaagga ggaggaatta
     2701 aatggaggtg gtttcttata taaaatcaac aatttttaaa tggtagcgct tatcccgaac
     2761 tcaggttgaa agtaatattg tgagataaaa tataaaaaat tatatgccaa aattacaact
     2821 tgccactgca ataggaatta ttttattctg gattgcattt tttacaatcg ggtttgaaag
     2881 ccctgcatac ccatcctatt acacaaaatt tgagcatagc tttcctttgc ctgatgcttt
     2941 tttgtcgctc atgctcatac tcgcttattt aaaaagaaat accaaaagtt ggaaaaaata
     3001 tacccttatt gccgccggtg ccatggtgtt tttaggcttg tgtgatttta gtttcaatgc
     3061 attgaatggt atgtatcaaa tcagcatcac agacacgttg ttgaatgcat ttatcaatat
     3121 atggtgtatc agtttcggat tgtatcagtt ggtgaatgta aaattggatt aaaaatactg
     3181 taccttatca tcataatatt tactcggcat tatctcattt ttcatataat tttcataaca
     3241 actcagcgtt atacaaagaa agtaaaaatt atattttagc aatttttacc ttatatttga
     3301 ctacgcgctg atttgaacat gaaaaaatat aatccgattt atttacttgc attcctgttt
     3361 ggcattttca ccttgtctat attttttact tcctgtaaaa aagacaaact aaacactact
     3421 tcaggattgc agttttcgaa tgatacactc acctttgata ctttgtttac cactttgggt
     3481 tctactactc gattttttac gatcagaaac aaagaaaaca aagctatcaa aatttcaaat
     3541 attcatttgg gcggtggaac tgattctcgc tatagaataa atgtagatgg tgatgcaggc
     3601 acttctttta cagatattga gattccggcg aaagacagct tgtatgtttt tgtggaggta
     3661 accattgatc ccaatgacca gacacttcct tatttagtct tggattctgt tcagtttcaa
     3721 accaatggca ttaaccagca tgtcatcttg cagacttttg gccaaaatgc gcatttcttt
     3781 tatgccgatt ctattactgc aaatacggtt tggaataatg atttgcccta tgtaattcta
     3841 aattatttgc aaattaatca aggagcacag ctttgtatta acggggggac aaaagtatat
     3901 tttggtcccg gtgctgccat ggtggtagag ggcgacttga aaataaatgg tacggatacc
     3961 aataataccg tgttgtttag aggtgtacgt ttggatgaag atattgcagg cagaccttat
     4021 gatgattttc cgggacaata tgccgggctc ttctttttac gcaacagtac aggcaatatc
     4081 aattatttag aaatgcgcaa tgctgcttat ggcatcaacg tgggtaatat taaaactacg
     4141 gatgatccaa ccgtaaattt acaaacgcta ttagacatga agtggtcgaa tgctccatac
     4201 gtggtaatca aaaacagcaa aatatataat caggcatact atggaatatt tggctttctg
     4261 ggtagaattg aggctgaaaa tctattagtt tacaattgcg gtacaaatgc tgttggatta
     4321 tattgtggtg gtgagtatga ctttactaat tgcacctttt ataccagagg aagtgcttat
     4381 gtttctcaca caaaagaacc tttgttttat atcaatgatt acttcaccta cagcactagt
     4441 tcgccgtcta ttttagcaga ctctacacga gcttatttca ataactgcat tttatacggc
     4501 acgctggaag aagaaatcgt aacagatagg gaaacaaatt ccacacacgc gtttgatttg
     4561 ttatttacaa attgcgattt aaaaacacaa caaacactat ccttgccttt ttacaatact
     4621 tgtaaaacag atgacccgca atttaaagat gtttataaaa atgattacca tttaaaaagc
     4681 ggttcgcctt gtatcgattt cagtaatcct ccatctactt ccacagatat tgacggattt
     4741 tctattacag gggcaaagag agattgcgga gcatatgaat atagataaac aggttatggc
     4801 tatatccgct caagtttact caacgttgaa ttaacatcat tttacataaa tttacatttt
     4861 cgctggataa atttatcagg taaagtatgc ctgacagtag tacgtataga ataataatta
     4921 cgaatccatg cgatgagaaa tgggaaaaca tgcagcctga tgaactgggg cggcattgta
     4981 tattttgcaa taaaacagtt gttgatttca cgttattgga tgatgaggct gtaaaaaaat
     5041 atttttgcag tataagaata tacccacctg tggcagattt ttcaaaaaac aggtcgatac
     5101 cataaggatt gaaatacacg aacaagtact ctatgccaat atcgcgtttt ggaaaaagtt
     5161 tttagtagtt ttcatgctct gttttagcac gcaacttttt gcagtcgagt ttattttgac
     5221 agatggttta ctgaccgatt ctactggagt ttttatttca gacagcagtc aattaaaccc
     5281 gacagatacg gaactcaata ccgatacaac accattatta catgtagata ccgaaagtat
     5341 atcacctgaa ataatattta atactattca tgcaggtaat attttgtcag gattttgtac
     5401 tattattagt ggcggtgttt ctatggaaat tgaaacaact aaagttggtc cattaaaaga
     5461 tgatacaata gaaacaaagt catcaagttc aacgacaatc aggaaatctg tcaataaaaa
     5521 caaccgggaa agcaaaccga tagagctgcc tcatcaagat ggtatgttac ccgaaacagc
     5581 cttgtatctt cccaatagga aacgggtaag ttaaacaggt tgccttgttt ttaattaatt
     5641 tttcatcttc gataaattac ccttaataaa taactcttgc ggaaagaaac aagaaaaaat
     5701 tgtattttgg caactttgta ttgtcgtgta aaattgctga aatctatata cttttaatag
     5761 aacagcaaac agatacaaat gaaaatactt tagagtaaat aagatagttg tatgcatacc
     5821 attctcccgt ttttcttagc tatgctcgcg gtcatcgtac tgctcaatat gtgggcaaat
     5881 aaattgaaaa tagcctatcc tattttattg gtcttggctg gtttgctgat tagttttata
     5941 ccgggtttgc ccatgctaaa tattaatccg gaacttatct tttttatttt tttaccaccc
     6001 ttgctcttcg aggccgcgtg gagtgtttcg tttaaagaaa tgaaaaaatg gtatcgcatc
     6061 attttaagct ttgcattctt ggttgttttt tttacggctt tcgccgttgc tgttgtaagt
     6121 aatcacttta ttcccggttt tagcattgct cttggttttt tattaggggg cattgtatca
     6181 ccacccgatg cggtaagtac ggccgcaatt actaaattcg tgaaaattcc aaaagcaaca
     6241 tctgcaatct tagaaggcga aagtttgctc aatgatgcct cttcgcttat catttttcgt
     6301 tttgccttgg ttgcagttgg aacagggcaa ttcatttggc aagaagcagc cgtcagtttt
     6361 gtatggatgg ttgtaggtgg tgctgctatc ggtttgttag cagcttggtt ttttatgcag
     6421 gctcacaaaa gactacctac agatgctcca tcagatatcg tactaacttt tattgaacct
     6481 tatttcatgt attgggtagc agaacaattg catagctccg gtgtattagc cgtagttgcc
     6541 ggaggattat tcatgtcaac aaaaaggctc acgtttttaa atgcaaccag tcgcattagg
     6601 ggattgagtg tatgggaaag ttttgttttt attctcaatg gtatcgtttt tttaataata
     6661 ggcttaggat tgccggaagt tgtgcaaggg atgcgttcaa acggtattcc attaggtact
     6721 gcaattggct atggtatctt ggttacgatt gtactgattg ctgctagaat gatcagttcg
     6781 tatgccgcca tgatcgcaac caggattttc aggcctactg tttttaacag ttttgccaat
     6841 aagcgtattc gctggtggtt gccattggta ttgggctgga cgggtatgcg cggtgtggtg
     6901 tctctggctg ctgcgctttc catacctgtt actttagcag atggctctga tttcccaaaa
     6961 cgaaatctaa ttctattcgt aactttcgtg gctatcttgt tgacgctatt agtacaagga
     7021 cttacgctgc catatataat caggaaaaca agaatattcg atcatgtttt tgcaacggag
     7081 catgaggagg aaacccggaa aaaaatgaaa cacgggttga agaaacaggt gtatacctac
     7141 atgaaagaaa agcatgaaaa taaattgata gagtatgaag ggatggaact gatattgaaa
     7201 caatgggagg aacgggcaaa agccgagaat gacaactggg ttacggatca tactaaagct
     7261 ctgtttttag aaattctgga aatacaacga gagtttttaa tggagctcaa taaggatcct
     7321 gaaataaacg aagaaatcat ccgtcaacaa ctttaccaat tggatttgga agaagaacgt
     7381 tggagggtag tctgataatt tttacaatta aaaagatgaa aataataatt acaggtgcaa
     7441 cgggaatggt cggcgaaggt gtacttttag tttgcctcga acacccggaa gtaagcgaag
     7501 tattgtctgt tagccggaag ccgatagcac atcagcatgc caaactcaaa gaactaatct
     7561 tgtctgattt ttcaaaactg gatgagcatg aagaactatt gaaaaattat gacggttgct
     7621 ttttttgtgc aggagttagt tctgtcggcg aaaatgaaga atccttcaca aggaaaacat
     7681 atgattttgt agtgccattc gcgcgcttgc tctccgggat aaataatgaa atgacattca
     7741 tttacgtatc gggtagcaaa acagacagca cggaacaagg taaggttatg tgggcaagag
     7801 taaaaggaag aacagaaaat gcactggtga aacttccatt taagtcagta tataattttc
     7861 gcccgggctt tatgaagcct gtaaaaggtc aaaaaaatgt aaatgctgtc taccgtgtgc
     7921 ttgaagtctt ttatccattt tggttgcttg ttcttcccaa ttggacgtgc agcatgcgcg
     7981 aagttggatt ggcgatgata cattgtgtaa gtaggggata taacaagcat gttttggaag
     8041 tagctgatat taaagttcag gctaaataat ttttcaaaaa gtttatagat ttttcaaaat
     8101 agattccatg agaaaagaac gttttgaagc gatatcagat gccgttttgg caattattgc
     8161 cacattgatg gtgttggaaa ttaaagtcgg agatttaacg gatgcgagtt taaaacaatt
     8221 cgtatttcaa ttgttagtct atgctgtcag ttttgcctat atcgctattt tatggatcaa
     8281 tcaccacaat atgcttcgtc atgttgaaaa aatagacatg aaaatagtgt gggtaaattt
     8341 ttggttgttg ttttttacat cacttattcc cttggcaaca tcaacaatta atgaagaatt
     8401 ttataacaca agaagtcact tgttttttgg gtcagtactg actgtcgtag tcttgtttta
     8461 ttccatttta gaagagctgg tttataaaat tacaaaacat aaatacaatg agagcaaccg
     8521 caagatgaac tggctgtcca tgtgcgtgtt tgctttaagt atcccgttga gttatgttag
     8581 tatttacata tcttcagcca tctttatctt gattccattc tcttattttt tattgcccaa
     8641 aaaagcaaac caacattttt ccaatttacc cggcaatttg aaccgatagt gaatcttgct
     8701 ttttgaaaaa attgaaccaa atcaatttta ttttctaaaa aatactttag attagcacgg
     8761 ctaaacaaca ggaaaaaatg cagagccttc aaagccttac tgtaaaaaca ataattaatc
     8821 tggtacgtag tagtttattc gttcataaaa cagatgccct tactgtaaga gtcggttttg
     8881 aacgtgtatc taatttaatg aagttcccga aatttgttgc tcgtaaagaa actaaagtag
     8941 ccggtatccc ggcttgttgg tttacaccgg atggacatgg gaaatcaaaa gtcatacttt
     9001 atttcccggg tggcggttac gtctgtggct cttataatac acaccgggca cttattgccc
     9061 gtattgcccg ttctagcggg tataaagtat tagcggtaga ttatagaaaa gcacctgaag
     9121 acccgttccc ggcagctaca gatgatgcac ttgccgcata taaacagtta cttcgtgatg
     9181 gctacgaaaa tatcgtgcta atgggtgatt cagcaggtgg aggcttggta ctcgcgttat
     9241 tgcaacttat tcgtaaacat aaactaaatc ctgctattgg ctcggtactg ctttctccat
     9301 ggactgacct tacattgagt ggagaaagta tgcacactaa aaaagatatt gatcccttta
     9361 ttaagcccaa tttggtagaa atatttgcca ggcgatattg tggagaaaat agcccccgaa
     9421 acccgttaat ttctccacac tttgcagatg tttcaggatt tccacccacg ctgattcacg
     9481 tgggtagtca cgaaacatta ttagacgatt ctgttaggat ggcacaaaaa atgagtgagg
     9541 ccaatgtaga tgttaaattg tctgtctaca aaaatatgat acatgtctgg cattttttag
     9601 gaggcattat gcccgaagcc aatcaggcta ttcaggagat aggagagttt gtaaaatcca
     9661 ttaaagtaaa caagaaaaca gcccatttag atgctttagc tgtttattaa cagatttcaa
     9721 tttcaaatgt attaacttgc gtctgaacta agcggatagt ttattagttt tagtatttat
     9781 gaaaaaagat gtgcgaattg tatttatggg tactcccgaa tttgcagtag caagcttaga
     9841 tgctttatgc ctgaatggtt tcccggtagt ggcagttgtt acagcaccgg ataaacccgc
     9901 gggaaggggt aacttgatgc atcagtcagc cgtgaaaaaa tatgctttag aaaaacagat
     9961 tcctgtatta cagcctgaaa aactgagagc cgaggatttt ctaaaccagc tcagggaatt
    10021 acatgcaaac ttatttgttg ttgtagcctt tagaatgttg cctgaaattg tctggagtat
    10081 gcccgagctg ggcactttta atttgcatgc atctctcttg ccacaatacc gaggagcggc
    10141 acccataaac tgggcagtaa ttaacgggga gaaggagacg ggtgttacca cgtttttttt
    10201 gcaacaggag atagataccg ggaatattat tttgcaggaa aagataaaaa ttgaggatac
    10261 tgataatgca ggtacagtgc acgataaatt gatgatattg ggtgcagatt tagttgttaa
    10321 aacagccaac ttgattgcga atcaagcagt aactcaactc cctcaaaatg aagccggatt
    10381 atctggtcat gaactgaaac atgcgcctaa aatatttaaa gacacttgca aaatagactg
    10441 gaatcagaaa gccgaacaaa tacacaattt ggtacggggg ctttcacctt atcctgctgc
    10501 atttactttt ttggataata aatcggtgaa aatatatgcg tctgcgatac gccatgccgg
    10561 tgctgaagaa atgcagcgat ttcatttaac cgcaaatata tataccgaca aaaaaacatt
    10621 tttatctttt tcctgtgcag atttgttttt ggatattctt gaaatacaac tcgaaggcaa
    10681 aaagaaaatg aagatagatg aatttttgcg tggacataag tttagccagt aagatatgat
    10741 gaagaataag gtatttttac agtatggtag ccggcaggtg ataacacctg ccgagggcga
    10801 gggatattaa gtcatctata cctataccat taatcctcaa tttacaaaag aatcaaatac
    10861 taattactga tgccaacatt aaaaattata attcaagttt tttttcaaat agcaatattc
    10921 agtatctatt cattatcaag atcacaaaca atatctaatc agattgaaag attcggtgaa
    10981 ttacaaattg atttagattc aatttctgat tataacattg tcagattgga tagcacgcaa
    11041 gatataaaat acttaagcat aacggctaca aacaatagtc tttcacaaga ttcaattaat
    11101 ttaagtccgt ttaagaactt agtatattta aaaatagacg gatataaact aataaaccta
    11161 aaacgcctat ttctaatgct aaaaaataat aaaaaactac aagtactaat attgaataat
    11221 aattgcatag aatctatccc ctctaatatt cagagtttaa aaaatttaac tttcttagat
    11281 ttaaattgga attctatttc aagtttacca agaaatctaa aaaagttaag ttcgctaaat
    11341 acactgatat tgggatccta caaaacaact cattctccca gtaaactttc taataaattt
    11401 caagatgtac ctaaagtagt attaaaactt aaaaaattaa atacgtttat aattacaaac
    11461 aataagttac aaaagataaa caaaaaaatg attgataaat taaaaaaaat taagcactta
    11521 ggcattttaa atggaaatta ttttgaagat ccaaacagta taaagtcaga attggaaaag
    11581 tataacatag aaatttggta atacattacc tctcctttat catgagttaa acttccaaaa
    11641 tgatgaaaaa atgttgagaa atataccacc cttagtaagc aaatgcataa tcttgaaaat
    11701 aatatagtat caaaaaaaga atttaaaaag aaaataaaga aacaataaaa tgaagcgaat
    11761 atttttgtat ataattttta taatacagcc attaagtttt tgtgcttcag acttaatttt
    11821 tgcatcatat aataatggta caatttgttt tgagaataat attgcaatag ttaaactcga
    11881 tattcgcaaa aataaatatt tttattctat agaaaatgga acccaaaaga gaagtttgtc
    11941 ttatggctat ttcaatttct taaatgattc aattattcag ataacttcag atttccttga
    12001 tacgacattt gttaattcaa ttaattacta tattgagaat aaaaatcgtt tcttctttta
    12061 tgataatcat gataagtcaa agttaaaaat aaaaggaaac aagttgttaa tttatgacat
    12121 tgatacacac gtagatttct tggatacgat gagtactaat gatagtttat taaattattt
    12181 tgttaatcat taccctattt atccggatag ttatttttat aaatatgaca tatcgaatga
    12241 tagtaatttt attaaagatg gaaatattat attcccattt gacatgggaa ttatactaca
    12301 aaatagcaat aattatgtga agatatttac taaggatttt tggatttcta ttagttcaaa
    12361 caatataaat aggatttact ctaacatagt tatgaaaaac cacataatat caaacacaat
    12421 taaaggtaat agtatattta tttacttttc taaaaacaaa tagaaagata taatgcctac
    12481 taaactctgt ttttgaaagg caataaataa gtcaaatctt caaagtaacg ataaaccatg
    12541 ctcttaaaaa ttttcaaaat aagcttgatt gtttttgtat caattgtatt tattattatt
    12601 gtttcctttc aagttaaggc agaactctac atgcataagc aagataatac ctatggcatt
    12661 ttgaaaaaca aacagagaaa aagttttctg ccatttgtat ggttttattt tattaataaa
    12721 aataaagata aaacaataac agggaaacaa tcgcttgagt tctactttaa agatgctggt
    12781 attcgtggag ttgttgtctg ttcatacata tatgatgcat acatgtatca tgtttattca
    12841 ggtagttggc atttaccaga ttcgattact acctcaattc aataacatgt tttgataatt
    12901 gaattgagta ctaaaatgcc cccatcttta aaaaagttat aaatcttata atataaattt
    12961 caggctgttg ttgcttgtaa attacaacta tgatgtgata ataaaatttg aatagctgat
    13021 tacttttact tttagacatg caccaaatca aattattcgt caacttatac agaccaaaac
    13081 ccgagtccgt acagcaaccc acgcatcagc aaagaggaga aattgggcaa ataccaagca
    13141 ctttatggcg gcttgtttaa agaagtattt gaagagcaag ccactgaaca attccgccat
    13201 tttgtattgc tgaattcaag taataaatac aactcgcatg aaaatattct taggatagat
    13261 gaattattac attcacataa gttcagccta taacatgtga atatcaaatt ttggaaattc
    13321 atcaaagttt tcgttatttt gatttgacca tcaggtctaa aaaaatctaa cttatggacg
    13381 atgattcgga tttaaagaat cgtatacttc tagcattaag cggtgatgca gtactacttg
    13441 aagacttcaa tacctctgat tttgtcgatt ttatcgagga agagccgagt tatgcagggc
    13501 aaattatcgc gtctttagaa ccacacaggg ctgttaaaac gttgaagttc ctggatatca
    13561 agcttcaaaa aaaattaata cactcgttac aacccgaagt agtagccacg cttatccagc
    13621 aaatgcattt tgatgacaga acggcactac tgagtgagct gccgcctgaa gtggtaaaac
    13681 aattggtatt acatcttagt catgatgatc gaatggaaac gctttcctta ttgggttatc
    13741 ccgaagacag tgtgggcagg ttgatgaacc cggattatat tgaagttcaa gttacagata
    13801 ccgtggaaga tgcattgagt gcaattcgca gttatggaag agaaatagaa aacatcaatt
    13861 ttatttatgt agtcgataag caggaaaaat ttttagacga tatcaatatt aaagaactgc
    13921 ttatcgttga tttgtctaca aaaatttcta cgctgatgga ttctaaattt atatcactaa
    13981 acgtgaatga tgatagagag tatgcggcat ccatgttcaa gcgtagcaac cgggccgtgt
    14041 tgcctgttgt tgataattcg ggcgttttga tgggaattgt taccatagat gatatcttga
    14101 gagtagagtc ggaagaaacc accgaggata ttcacaaact tggtggcacg aaagccttgg
    14161 atggcgcata tatttccgcc tcattatcta atcttataaa aagcaggttg ccttggctac
    14221 tggtcttata tttaggcgaa atgctgacga tacatgtgat gaactatttc aacatagaaa
    14281 tcaaggaatc tgtcataatc gcttttttca ttcctttatt aatttcgagc ggagggaata
    14341 ccggctcgca ggcttctttt ttaattattc gcgatctggc tttagatgaa atcagctttg
    14401 acgattggtt taaagtattc ttaaaagaat tggcaagcgg gctaatatta gggctcgtgt
    14461 tagcaatagt aggagtgggc agtattttaa tttggcaaaa tatttaccct atattcggag
    14521 agaattatat cttgttatca ttaacagtag gcttgtctac attgtgtgtg gtgctttggg
    14581 gtacattgtg cggctccatg ctgcctttgc tcttaaaaaa aataggactg gatcccacga
    14641 catcatctgc accatttatc gccactattg ttgatgttac cggcttaatc atctatttta
    14701 cgattgccta taatttgctg atacactagc ccgacaaaac aacgattttg gatgaggaga
    14761 attagtgatg tataaaccag ttctccttag acttgtacac aaataataca cattgctcac
    14821 aaaatcaaca cttttagaac agctaatctc ctttctatag taatactgta ctaaattcta
    14881 taccgttttt tagggtttac tttgcggtat aattattact tatgaaatac acacacattt
    14941 ttatgcttgc acttacattt ctgtttgtag cttgcaacac aacatctaaa aaggatgaga
    15001 atcagaatgc acaaaaagga attgccataa atattgatgg ttccagtacg gtttatccta
    15061 ttaccgaggc agtagccgaa gaatttaaaa aacaatttcc tgacataaag gtatcagttg
    15121 gagtttccgg tacaggcgga ggatttaaaa aatttggaag aggtgaaaca gatatgaatg
    15181 atgcttccag acctataaaa cctgaagagg acagcttggc taaagccaac aatattgcgt
    15241 acgaacattt tttaatagct tatgatggtt tagttgtggt tgtaaatccc caaaatactt
    15301 ggtgtaaaga catcactgta gctgaactaa aaaaaatatg ggaacctgca gctcaaggaa
    15361 aaattatgaa atggaatcaa atcagggcgg aatggccaaa tgaagaaatt catcttttcg
    15421 ggcctggagt ggaaagcggt acctatgatt tttttacgga ggcaattgta ggtaaaagtc
    15481 attccagccg tggcgattat actgccagcg aagatgataa tgttttggtg caaggtatag
    15541 cctcagataa agatgcactt ggatttttcg gaatggcata ctacgaagaa aatacagata
    15601 agttaaaact ggtaggtgta gatgatcaaa aagaagataa tggtaaaggt gcaattttgc
    15661 caactgtcga aacagtgaaa aataaatcgt atgcgccact ggggcgtcca ttatttatct
    15721 atgtaaatga tattgctgca gagaaaaagg aagttcagga ttttattaat ttctatctga
    15781 ataatatggt gaaattatca agtgaagtag gctatgtgcc attgacagac gaagaagcaa
    15841 atgcagaaaa atcaaaatgg atggagttct taaaacaaca cggcggtatc aaatagtccc
    15901 ttgattttat tgtatgaaat taagagaaaa aattattgag tcattactgt ttttgtctgc
    15961 cctcgttagt gtattaacaa ccatcgctat actttgggta ctgctttcag aatcgtctgc
    16021 ctttttcaaa caagtacctg tatggaagtt tttgacagat agccagtgga cacccttatt
    16081 tgccgacaaa cattatggca ttatgcctct ggttgcgggt tcatttctta ccacggttat
    16141 cgccattatg gtagcattac ctgttgggct atccattgcc gtatatctca atgagtacgc
    16201 atcaaaaaaa atccgcaata cactaaagcc ggttttggaa attctggctg ccgtacctac
    16261 agttgtatat ggttattttg cactgatgat tattactccg tatctgcaaa aaataatccc
    16321 gggcttatcc ggatttaatg cgctttcgcc gggtatcgtc atgggaatca tgattattcc
    16381 tttagtatca tccttaagtg aagatgcgtt gaatgcagta ccaaattctt tacgagaagc
    16441 ctcgtatggc atgggggcta caagatttca aacggcattt aaagttttaa ttcctgctgc
    16501 ttcatcaggc attattgtat cagtaattct tgccgtttca agagctttag gcgaaacgat
    16561 gatagtggcc attgccgccg gacaacaacc tcgtttgacg ctcaatcctt tagttccggt
    16621 agaaacaatc accacgtata tcgtacaagt cagtatggga gatgtggtac aaggatctct
    16681 tgaatacaaa actatttttg ctgcgggtat tgccttattt gtatttactt ttttattgaa
    16741 caatatgagc ttttgggtaa aaaaacgctt tcaggaaaaa tacgagtaaa tgaaatcaat
    16801 agcacaaaaa aacaggataa aagatacgtt atttaaatac ttcgggatat tcagcacttg
    16861 cttcgggctg ataatgctga tgatatttat taccaatatt cttattgtcg gattaccccg
    16921 tttgaacttg caatttttta cttcctttcc gtcacgcaaa gcagaggaag ccggaatctt
    16981 ggtagccttg gtaggttcac tttggattat gatgctcacg gcaatcatgg caattccact
    17041 gggcattgct tccggaattt atctcgagga atatggcaag aaaaataaat ttgctcagtt
    17101 tattgaaatc aatatcacca acctcgcagg tgtgccgtct attatatatg ggttgttggc
    17161 actggaaata tttgtcagaa ttctaggatt aggcaaaagt attttagcag ggagtctaac
    17221 cttggcattg ttgattttac ccatcataat agtagcaact cgtgaagcgt tgaaggctgt
    17281 gcctcattca attcgcgaag cctcttttgc ccttggtgcc accaaatggc aaactatctg
    17341 gcatcaggta ttgcctgcat cgtcaggcgg aattttaacc ggtgtgattt tggcattatc
    17401 cagagctatt ggtgaaacag ctccactcat tgtagtggga gcagtattgt ttgttacaac
    17461 attacctatg tcgccaatgg atgatttttc aatcttgcct atgcaggttt acaactggct
    17521 atcccgtcct caaaaggcat ttacggttaa tgctacagcg gcaataatcg tcttgttgtt
    17581 tataaccttt atgctgaatg gaattgctat atttttaaga aacaaatggc agaagaaagt
    17641 aaaatggtag tcggttgagg aaaatggtaa caaagtaaaa aaaatgaaaa aatgaaagct
    17701 attacagaag atataaatgt atattatggc aatcaccatg ttataaaaaa tacgtcactg
    17761 gatattgctg ccaatactgt tactgcattc atcggtccat cgggttgcgg taaatctact
    17821 tttctcagat tgttgaatag aatgaatgac tatttatcgg aattccgcat gaaggggcga
    17881 gtgcttattg atggtgttaa tatttatgac aagcaggttc atgttgagga gttgcgcaag
    17941 caagtaggta tggtttttca aaagcccaat ccttttccga aatcaattta tgaaaatgtg
    18001 gtctatggtt tacgcataca gggaataaat gacaaaagca aactggatga aaccgtagaa
    18061 aaatcgttgc gccatgctgc attgtgggat gaagtgaagg ataatctgga gaaatcagca
    18121 ttagcattat ccggtggtca gcaacaacgc ttgtgcattg cccgtactct cgctgtgcag
    18181 cctactatta ttttaatgga tgagcccgca tctgctttgg atcctatatc aacggcaaag
    18241 attgaggaat tgatttacga actcaaaaat gaatatacta ttattatcgt aacgcacaat
    18301 atgcagcagg caagccgtgt aagtgataaa acagcatttt tctatttggg agatctggtg
    18361 gaatacagtt cgacaaaaaa aatgttttta aatcccaata aagaattgac acaaaattat
    18421 ataaccggca gattcggcta aaatacatac aacacatgaa ctacttagaa caagaaatat
    18481 atcaactcaa aatttctact ttagaattgt gggatttggt tttatcccag ctcaataaat
    18541 cgaaagaagc attgcaacat tacgacaaga atttagccag agaaataaaa gctacagaaa
    18601 aacgcgtaaa tgcgctcgaa cttaaattag accgggattg tgaaaatatc atagcactgt
    18661 ataacccggt ggctgtagat ttacgttttg taattgccgt actgaaaatc aatatgaatc
    18721 tggaaagaag tggtgatatg gcagaaggta ttgcaaaata tattctcaaa tcggagtcac
    18781 agtttgccag tacactcatg gagcaagcac gcattatcta tatgttggat gaagccatag
    18841 taatgctgga aatggtgcgt atggcatttg aacaggaaga tgcaaaacta gccagacaag
    18901 tttttgaaaa agatgaaatt ttaaacagca tcaacaaatc tatcgaacag gtgataggtg
    18961 catatattat cgagtatcag caagatatcc ctaatgccct gcatattcta tcctctatta
    19021 gaaaaataga acgcgttggc gatcaatcca agaacattgc tgaagaagtt attttttatc
    19081 tggaagccaa ggtgctgaaa cacatgaaaa aacgtgaatt gcgtactgaa aatcagcaag
    19141 acattgaagg tcaatcaaag ggaaatgact agtgcttttt ccgagaggtt gtaataattc
    19201 aatttatttt cccttagaat tttttcaaag atatgctgat gcctgtaatc attactcgat
    19261 ccgataagta ttgatttaaa ctgataattt ttcagcacct gctcgatgtt cagataaata
    19321 ttatcactca ggtacaagta atcaattttt aaaggtgaag caaaatcttt atactgattt
    19381 tctttgttta gcaggtaaat tgttttgtct ttgaagaaca gcaaatttga ataatcctga
    19441 agactatcta ataattctag tttagtttgt tggatgtcgt aataacgctg tgcgtttaaa
    19501 atattaaacg tataatcttt cgtacttaaa gtatcggata ccaatattgt cgatgtagaa
    19561 ttttcattga atgcaataca atcttttttc ggaatggaat aaaaaatcag ttcactttgt
    19621 tgcacttgtt tgatattctc aaaataggaa atagctaatg ttattaaaca aagcgataaa
    19681 catgccatca ggtattttgg ttttttccac tctataaaaa aagtaaacaa ggttacagca
    19741 acaaataaga ttacaagttg tagtggaagt aaatgcaaag tatttaattt tccaaatggt
    19801 aaatgtgcca catacgcagt taatgcaata aaaatataca tgatggtact gagaaataag
    19861 ccaagaacat agccaacaaa tggaatccag ctaaaaaaga caagtccgat acagagccaa
    19921 agtataacag cagataatgg tatggcaaag atattggcaa ttaaaaaata caccggaaaa
    19981 ttgccgaaat agtaaatgct caagggtatg gtaaacactt gtgcagctaa tgaagccgcc
    20041 cacaatttcc atattttatc cggtaaatta tgtcttacat acaaaaggga ataaaatggt
    20101 ttgtagaaaa tgaataagcc caacatggcc aaataggaga gttgaaagcc gacatcaaac
    20161 aatagatttg catcgagtaa taactgaatt aatgctgagc ctgccagcaa attgagatta
    20221 ttgaaataaa ttttattttc tgtaccaaat gtaagcaggg taaacaataa ggccgctctc
    20281 acacaagcag aagataatcc ggttacgaat gcaaaagtcc agataaatgc aatgacaaag
    20341 attgcttttg taatgcgcgt tttcttgctt ttgtctttta tcagacttaa gaaaaacatc
    20401 agtatgccgt aaatgatgcc gacatgcaaa cctgacacag ataaaatatg caaaataccc
    20461 gtgtaagcaa attcattata caactcatca tcaatatcat cgctgatgcc caataaaatg
    20521 cctttggcaa atgctaaatg agatttttca gggatgtatt tcgtaagtat tttttgtgca
    20581 taaatactaa gctgatacgc atacctacga acagcatagc gcattttttt accggttact
    20641 tttagttgat cagcctgtaa atactgttgt tgaaatatag atttgtgtgc taaataattt
    20701 ttatagtcaa actgatacgg atttttaggt tcttgtatgg gttgaaattt taaagggaaa
    20761 atgagcttat caccaataga caaatgcagc aaactactgt cttttgcaaa ataaaccaag
    20821 agttttccat gaaccggatg cacattaaac tgactgtcga caagctcact aattgcgaaa
    20881 acagtcttgt atgatttttc tgtttcttta atgggttgag taatataagc tgttgcaatg
    20941 cctgaggtat aagaagaaaa atgatttttt tgctgactga gatgtttggc actgattatg
    21001 gcaccaccca caaaaaacaa taggatataa aataaaattg aaacacgatt tttttgtccc
    21061 ggattattta ctcgaacatg taacagcaac aggatgatga aaagcagaag tgaaatactc
    21121 gataatacaa gtaccgaaac attgagcaag tcaaatacaa aaataccggc aatcagtggt
    21181 aacaccgttt tgattaaagg catttgaagt aacttatgtt tcatatacga atataatcat
    21241 agttttcaaa ttgatgctgt tttaattagg taaaaagttc tagggggagc ttttatttat
    21301 tatttataaa ttggatttag gttaaataaa ataagctgat aaaaaagttg atttctcatt
    21361 tttcctccac tttaataagg tggaggaatt aaaaggaggt ggtgcttaat agtataaatc
    21421 aattttttta tccagatctc agtataatac atctaattag ctatccattt tgaattaaat
    21481 attgatgttc ttcttttatt ggattaattt ctaaagcatt tggattgtca tttatccggc
    21541 tttttgttcc ttttttctta attttggata tgtcattt
//
LOCUS       DAOQII010000007        21209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1125_length_21209_cov_6.798525, whole genome shotgun sequence.
ACCESSION   DAOQII010000007 DAOQII010000000
VERSION     DAOQII010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21209
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1125_length_21209_cov_6.798525"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLF48_00775"
     CDS             <1..274
                     /locus_tag="PLF48_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF47881.1"
                     /translation="YDMSMREGNRESFSARVQQVDQEPKLNVSAIKTPTLILWGKQDI
                     LIDISMAANFTKIAGSKLIVYDHCGHSPQEEIPAKSVEDALQFLKQ"
     gene            complement(448..1503)
                     /locus_tag="PLF48_00780"
     CDS             complement(448..1503)
                     /locus_tag="PLF48_00780"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNF47882.1"
                     /translation="MKINKNHIKGRGAQSNSEHRYSSYIYDKSEADEQTDENHSTKYI
                     EVFPKTFINKISSPDIPMDYSMNPYEGCEHGCTYCYARNTHPYWGYSAGLDFERIILV
                     KKTAPDLLKKELNKKSHTASPIMLSGNTDCYQPIERKLEITRSLLKILLEFKHPVGII
                     TKNSLIERDLDLLIELNQLQLVQTVISITTLNEDLRRQLEPRTSTAINRLKTVELLAK
                     NKIPVMVNIAPVIPGLTDMEIMKIAEKSAEAGAIGISYNIMRLPGEVEQVFTDWVKKT
                     MPDRANKILNQTKQVHGGDLQDKKFGRRMRGEGVLSDSIKAQIQIARKKYFQERELPA
                     LRTDLFINEHNRQQKLF"
     gene            complement(1550..2311)
                     /locus_tag="PLF48_00785"
     CDS             complement(1550..2311)
                     /locus_tag="PLF48_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNF47883.1"
                     /translation="MSAPFIIQLPQFEGPFDLLLFFIERDELNIHDIPIYKITNDFLD
                     YIQHLERLNIEIASEFILVAATLMRIKAKMLLPRKELDDQGNEIDPRQELVQQLLEYK
                     RFKDVVEVLKDMEGSRSLRYARGNQKNEVDQLVKLMAEDADLETVSLFKLLKAYMRSM
                     EKFKNRTEKVVHTIAPFKYSIEEEKSKLVSFIQLKERANFSELFQACESKIHAVFILL
                     SMLELIQQKMLFLVLGEGYNNFWVSQASLESDTAE"
     gene            2368..3636
                     /locus_tag="PLF48_00790"
     CDS             2368..3636
                     /locus_tag="PLF48_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47884.1"
                     /translation="MIVIYTPKVTNRIKYVFDFVFEQYFGMTYFVTENKDESINEDDI
                     LLVYAGINPVSGFSIFQDNLLLEETIAEQRIYVSRENNLPVFFQTDEHFNISFDIFSC
                     IFYLITRYEEYLPHTTDAHGRYLSSNSILAKKEFSFRPIVEEWLFYFKEELIRHFPQL
                     EIKEHTFRYIPTFDIDQAYRFLGRNWLKHPPNIFAKDALAVLFQKKQDPYDVYDFIFR
                     ELESYNLQPVFFFLLNDEGVNNSKVSPKSKKLEQLILQVASANYPTGIHPAYDALKEL
                     TLLQEAELLTAITGNKTNIARQHFLNIRFPDYYKELVKAEITDDYSIAYPDVIGCRAG
                     TTQAFPFYNLTENKVYNLQIHPAVWMDATFAYYQTEKKNEQIMKDFSDFVLHLRKING
                     ILVTIYHNDLLATDKYRYLFQTINQQVNKF"
     gene            3642..4676
                     /locus_tag="PLF48_00795"
     CDS             3642..4676
                     /locus_tag="PLF48_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF47885.1"
                     /translation="MKKLFFTFLLSTFFISIFNNCFAQDYKYSQFYNSPLNLNPSFTG
                     QIHSLYRLVANYRLQYLPIQKPAPYNTFSFSGDVGLLRDRLNGDILGVGAVFTNDRQS
                     SIKSNLMMVSLAYQKILTQKKNSYHYLSAGFQFGFTQRRIDVNNLAFESQFTGTGFDL
                     TLDNKENFFKDKYVKPNMNIGLMWTGRFNKMIGAYLGFSMFNIIKAKDTFFASDNERA
                     YRMNAHAGLNVTIKDFVLISPNMMYMRQAKAQQIIGGTSVAFSLNKDEPFRTAVSAGV
                     WYDGNQAVIASAGVSFSGVQIGLSYDATFRKDLSKADKSFGALEASIIYTSKPLEKKK
                     RYSPLLCPNF"
     gene            5435..7297
                     /gene="mnmG"
                     /locus_tag="PLF48_00800"
     CDS             5435..7297
                     /gene="mnmG"
                     /locus_tag="PLF48_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNF47886.1"
                     /translation="MFAEYDVIVVGAGHAGCEAAVAAAKMGSKVLLATMNMQTIAQMS
                     CNPAMGGVAKGQIIREIDAMGGYSGIVSDKTMIQFRMLNLSKGPAMWSPRSQNDRMQF
                     HLVWREMLENTPNIDFWQDMVFEILEENGKACGVKTSLGLEIRAKAVVLTNGTFLNGI
                     IHIGERQLGGGRAGEKSSKGITEQLVSLGFESGRMKTGTPPRIDGRSLDYTKMEVQNG
                     DDQIVGFSYKNIEKPKEQRCCWITYTNDDVHEILKTGFEKSPMFQGRIQGLGPRYCPS
                     IEDKINRFADKNRHQIFVEPEGWNTVEIYVNGFSTSLPEDVQYKALKMIPGFEQVKMF
                     RPGYAIEYDYFPPTQLKRSLETKLIKNLFFAGQINGTTGYEEAAAQGMMAGINAHLVV
                     NEKDPLILQRDEAYIGVLIDDLVSKGTEEPYRMFTSRAEYRILLRQDNADLRLTELSY
                     KLGFAEAERMKRVEEKQKATNEIIDLLKNSSIEPQEIDNYLVEKGTTVLPQKMKLEKI
                     VLRPQIGLLDMTEHVAAVKTLLGKYEDEFLEQAEISVKYEGYIEKEKELVRKMQKVEK
                     LKLKPDFDYNSIPSLSIEARQKLNKIKPETVGQAGRISGVSPADIAVLMVYLSK"
     gene            7313..9109
                     /locus_tag="PLF48_00805"
     CDS             7313..9109
                     /locus_tag="PLF48_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HNF47887.1"
                     /translation="MKKPDKINPCKKIIIPITSLFNCLIISFILSSCAQISAPTGGER
                     DKNPPKIKAENPPNKSLNYSGKEIKISFNEWLQPLTNIKSQVIISPNIDPFPKISIER
                     NILHIKFKDNVLLPNTTYSLFFGDNIKDNNEGNAFPNFKYLFSTGSYIDSLQIKGKII
                     PADGKLEDNTYLVIYSDLSDTAFTKTRPMYATKIDNNGSFSLENVKEGRYKIYTLTDK
                     NANYYYDLPTEKIGFTDSALVLNANLDTLSIHHFLPEAEKYRIVSSENTINGGMIKLE
                     LNKPYAVEKDEWKVSLKEDSTLTALSFPQEDGKKLLVYFPNLKSDTGRYQLMLFLNNN
                     LVDSLPVRIASAKWKKPQLFFTDTSAYKNLKVFEGKKLKLKSTNYTTSKPDTSKISLT
                     DSAGQKLSYEIRVLEDLSSLELKTDWKNGVPYKLTINDSAMIDLLGNYNKKQDFSILG
                     VSPKKGGNLLINIVLPDFKNDYIVILSDNSGKVWDRRILRDSQAIKMDYGLLPAGSYS
                     LKIIDDKNNNGIENSGSYMHKTLPEKSFTSKPLTIKENWDAEETVKADFSMPTIVPRK
                     TNNEDGGDGVIEKSEFKNEIKNIPGKKDIGKN"
     gene            complement(9106..9873)
                     /locus_tag="PLF48_00810"
     CDS             complement(9106..9873)
                     /locus_tag="PLF48_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF47888.1"
                     /translation="MKILSDNLLPSFCASVEINSLDDINIAEETPLQIDYYNFYNAIS
                     SVYSSKIEGEAIELDSFFKHKFLNVSFQQNYTEKTDDLFSAYQFIFSHNLTYDNVIHT
                     HQLISQHLLPSDQQGKIRTNLMYVVNKDGGIEYVAATPNIIQTELIALFEDIQFLLNE
                     PLDFKEVFYYAAYIHLAFVKIHPFQDGNGRTARLLEKWFLKEKLGEKAMGIQLEKNYF
                     NKLELYYSNIKNIGVEYIALNFEKAISFLLMTISSLH"
     gene            complement(9887..10213)
                     /locus_tag="PLF48_00815"
     CDS             complement(9887..10213)
                     /locus_tag="PLF48_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNF47889.1"
                     /translation="MKTILLLFISNTFMAFAWYGNLKNVNLKMTHAILLSWGIAFFEY
                     CFMVPANRIGFRTFSAYQLKLIQEVISLFVFVLFAYFYLGEKFKWNYAISFMFIFMAV
                     LFMFKK"
     gene            10281..12017
                     /locus_tag="PLF48_00820"
     CDS             10281..12017
                     /locus_tag="PLF48_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF47890.1"
                     /translation="MRNYLLFILAFVLVSCGDASKREFKLTPAKGGVYYGGTFRYNEE
                     EYFKTLYPLNITEVTAHRIIEQIYEGLVTFDDSTLAVIPNLAKYWTIDESKTKYTFIL
                     NNNVYFQDDACFPDGKGRKMTAQDVKYCFDRLCYHHPADNQGFWIFKDVVKGANEYDS
                     LTEQKIIPKDGVSGVRVINDTTLEIELYKPFSVFLPRLGLAFAKVYPHEAIEKYGELI
                     EHAVGTGPFYLKVVRANQAVFLERNKKYWKKDEYGNQLPYLNAIRVSFIKDKKNELLE
                     FQKGQSDLVYKFPLEMIGEIVNPDNKLQAAFAKFQLQYMPTMSLQYYGFQHQGKIFNN
                     INLRKAFCYAVDRERLCKYTLKGTGFPAIYGSIPPGTGTYDAKQVKGYHFNPVLAKEY
                     LAKAGYPNGRGLQKIILELNSGGGRNISVAEAIKKMLEENLNIKMELLMIPWAQHTEA
                     VESAKADFYRLGWVADYPDGENFLNLFHSKYVPADINTKTSINSFRYINKKYDEYLDK
                     ALNCVDPKEINEYYIKADQQMIDDAVMLPIYYDIDYRLLQPYVRNCPQNAMEFRDFSE
                     MYFIPFQTSTQK"
     gene            12020..12682
                     /locus_tag="PLF48_00825"
     CDS             12020..12682
                     /locus_tag="PLF48_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47891.1"
                     /translation="MSSVEQQAIEKRYRFRNIFSALPQFAKDSIYGLVHSYRYSKWNK
                     AGNPVPPPHVVKQDAIKSYGSKYNVKTFIETGTFLGDMLEAVQNNFDTLYSIEIVDFI
                     HELAKNRFRNNTKIKLLLGDSSVKLPELVQQLKVPALFWLDGHFSGGDTGFGDTGCPI
                     YAEIDTIFASPHKHILLIDDARCFIGEVGYPPLEEFRKYVLSKKPEAQFEVKDDIIRV
                     VY"
     gene            12707..13156
                     /locus_tag="PLF48_00830"
     CDS             12707..13156
                     /locus_tag="PLF48_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47892.1"
                     /translation="MKFLLYISVFILLSGFIKQSVYVDPTGTYILNNKTEYANSTKIG
                     KIQVKKFSDKKIILNFYINMGSPAFNSGSFLDTLSYEKNKAEYKCPLEDTSCKVTLYF
                     YKTGIKVIQETENKNSGCGFGHGVIASGFYKRFSTKIPIFEDPLKEK"
     gene            complement(13204..13446)
                     /locus_tag="PLF48_00835"
     CDS             complement(13204..13446)
                     /locus_tag="PLF48_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47893.1"
                     /translation="MRPVTHICTNCLKDIQLMLDGELDRHKENQLREEIEKCATCQAY
                     YQEQFNYKKLISQKVQRRSCGDDLKDALRLRIRGLG"
     gene            complement(13522..14106)
                     /locus_tag="PLF48_00840"
     CDS             complement(13522..14106)
                     /locus_tag="PLF48_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF47894.1"
                     /translation="MTVVSESLIHKERLFEKEFMPHADALYNFAYQLSYNDDDANDLV
                     QETYMKAFRFIDSYQEGTNSKAWLFKILKNIFINEYRRKKREPEKIDYDDVITFSDED
                     DDNSAAYFDLREDLFDNMMGDEITNALNKLPIEFKTVILLCDIEGFSYEEIAKIIDIP
                     IGTVRSRLHRARNMLKQLLKDYADQMGFEDNDEC"
     gene            14341..15396
                     /locus_tag="PLF48_00845"
     CDS             14341..15396
                     /locus_tag="PLF48_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNF47895.1"
                     /translation="MLLSLPVLFFAYLITLFWSADKQQFLQVIKNKIPFVLVPLSLIS
                     LQLLKPGQLQFLMNWFLLALLGACIWTFYQISLQDFDILMRLSVGQSVPTLVHHIAFS
                     VLLVFGILFCLRLYEMTENKTAKGIYLAAGIFFFLVNHLMIARSGLVLTYLSLVIYFI
                     YKKIEQKQYVKLALSIFLLVVIALINVYKNPYLKNKVDYTMTGKKAFENQDDSLNQYS
                     DTRRIISLQLGYKIFQQHKIVGVGIGDVKQEMQQLYQQKYPKFEEEVQAKIHSQYLFI
                     LAACGSILGILCFISFVYMAYYFLNENKIVWFILMLNILLVCLWEAFLENQLGTSMFL
                     IVFILGYLGNNKQIKSV"
     gene            15400..16479
                     /locus_tag="PLF48_00850"
     CDS             15400..16479
                     /locus_tag="PLF48_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF47896.1"
                     /translation="MLKIVHITMAKTWRGGEQQLACLMENKNLNQVVIGLNNSAFEKY
                     AAENNLQFYGIRNHLSFFSVLQFIKKLKPDIIHVHDSKAHTMVYCCQLIAKEKWKIVV
                     HRRVLFSPSSSWFTDKKYNDDSVKKIICISEAVQQVMQKVITEKSKLTVIYSGVDITK
                     FETKQDEKNKLNSTFYKAYQLPDHAQIIGVAAALENEKNIEEAIAIFKNVIEKNENAC
                     LVIAGNGSLFNTYKQLYENDKIRFIGFQKDMKTFYNSISTFLFTSKKEGLGTAVLEAM
                     SAGVPVVCHNFETAKEMIENGKNGYIYNNKNEAATIINTFLTDENKRSAISKNGIEMI
                     QQKFDKEIMISKTEEIYYSIVNIQA"
     gene            16476..17054
                     /gene="nadD"
                     /locus_tag="PLF48_00855"
     CDS             16476..17054
                     /gene="nadD"
                     /locus_tag="PLF48_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNF47897.1"
                     /translation="MKVGLFFGSFNPVHIGHLLVATRMREAVNLDEIWFVLSPQNPFK
                     LNAELADEQQRLEMLNLSINDIDYFKVCTLEFELDKPSYTHITLKTLKKQYPEIEFKL
                     IIGEDNVNKFQEWKEAKWISDNIEILVYARKSLSDKNENESLFKMKKINYGYIDISST
                     EIRQRIKENKLIDFYVEEKVKQYIMFHKLYQF"
     gene            17596..18612
                     /gene="nadA"
                     /locus_tag="PLF48_00860"
     CDS             17596..18612
                     /gene="nadA"
                     /locus_tag="PLF48_00860"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNF47898.1"
                     /translation="MTNETDIKDKLAELPERGFLDVQIDPTVDLFAEIDRMKKEKNAV
                     ILAHYYQTADIQDIADFIGDSLQLAQQAEKTTADIILFAGVHFMAETAKILNPTKKVI
                     LPDLNAGCSLAESCPAEDFAAFKKKFPGFAVVSYINCTAEIKALSDYIVTSSNAEIIV
                     NSIPEEQGIIFAPDKNLGAYLVKKLNRDMVLWDGACMVHEIFSLDKIIALQEKYPEAK
                     LIAHPECEAALLDMADYIGSTTNLLKYTMIDDSQEYIVATETGILHKMMEASPNKTFI
                     PAPPENNCACNDCPHMKLNTLEKVYLCLKYEVPELQMEETLRVAAKIPIDRMLDISAK
                     AGLK"
     gene            complement(18614..>21209)
                     /locus_tag="PLF48_00865"
     CDS             complement(18614..>21209)
                     /locus_tag="PLF48_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF47899.1"
                     /translation="KPSNETRLLEADILLRQNKTHAAKVIFYSLLPKTTDRLQATAYN
                     GLASCYYIDSELDSAEHAYLQALQIYSRIYDNKHTRYAQVLYNLGLIYGEQNDLNRSE
                     KYFTEALYLYEKRLPQNHPRIAEAHATLGGLFMNKDDVESALYHFNQDKKITEAIYGK
                     QATEAFYAYLNCGTTYALKEEYIDAKNELKTAYVISHKTFKNAHYQKALAALELSKVY
                     KNMHLLDSALFYLQEAEDMELVLNPVSYRLIDIYYELGEVHRLLQHYKISRQYFQKSL
                     DCSTQILGENNSYRIDAYRGMAINELAQHHLAQAENYINKAKIICTDKFGNITNKPDY
                     WQIELIRFKINSEKFELHKPSADDIKNEIKQLQSCLILAQNVKNSYYTQENKLYYNRK
                     IDELNQTALYLLTQFYPRQDTWFLNEVWFFAQSSKANLLRSRLQDFETEKSIPQLFRT
                     QYDDIKKQIYFFEQNGQYDSLYFYQKQYAELVQKIGKAYPKYFALRFDEHIISIKEIQ
                     QSLSANEQYFSFYTAGDFYYLYAIDKFSVKNIRLANTAFTDSLIGVLNTAVAEKKYHK
                     QKGTAIFKLLFSQVTLLEHITIEPSGNISFLPFDILSEDGQHYFLEDHTTVFSFSANT
                     FFHKKNTATESNCLAIFPNFSGSGLSVLNFQLEKESINKLNDVSILSGQYATKKAVVW
                     NLPKFGVIHFGTHLLYDTLRPLMSKLMLSNGEYLALDEIWKLQTPAQFVCLAACKSNA
                     GKWQQGEGLQNFAWAFEYAGAANVLATRWDAADEATAKLMADFYGLLKSGKNKTEALQ
                     SAKMHYLKNADAIAAQPFFWSHLILYGNHDEIKLKPKFLAHFWWMPVILLICFLIGIS
                     AIYKWKNK"
BASE COUNT         7239 a         3552 c         3748 g         6670 t
ORIGIN      
        1 ttatgatatg tctatgcgcg aggggaatcg agaaagtttt tcagcacgag tgcaacaagt
       61 tgatcaggag cccaagctaa atgtttctgc aataaaaaca cccacactca ttttatgggg
      121 aaaacaagat attttgattg atatttctat ggcagcaaac ttcactaaaa ttgcaggttc
      181 aaaattaatc gtgtatgatc attgcggcca ctcgcctcaa gaagaaattc ctgcaaaatc
      241 tgttgaagat gctttgcaat ttttaaaaca gtaatcaata tcattaagtt aaacatgatt
      301 ttatgcagaa taatccaaat agttgattta gcttttttta gaaccacctc ctttagtcct
      361 ccgccttgat gaagtcggag gaaataatga gaaatcaact atttttacag ccgattaact
      421 taactacatt gatagtaatt cctttattta aaaaagtttc tgttgccgat tgtgttcgtt
      481 tataaataag tctgttctaa gcgcgggcaa ttcacgttct tgaaaatatt tttttctggc
      541 tatttgaatt tgagctttta tagaatcgga taaaacgccc tctccgcgca ttcgtcttcc
      601 aaattttttg tcttgcaaat cgccgccatg tacttgcttg gtttgattta aaattttatt
      661 cgcccggtct ggcatcgttt tttttaccca atctgtaaat acttgttcca cttcgccggg
      721 tagtcgcata atattgtacg agataccaat tgcacctgct tcagcagatt tttctgcaat
      781 tttcatgatt tccatgtctg tcaacccggg aataacaggt gcaatattaa ccatcaccgg
      841 tatcttgttt ttggctaaca actctactgt ctttagcctg tttatcgcag ttgaagtacg
      901 cggttccaat tgcctgcgca aatcttcgtt tagcgtggtg atagaaatta cggtttgtac
      961 gagttgtaat tgatttaatt cgatgagcaa atccaaatcc cgttcaatta agctgttttt
     1021 tgtaatgatg cctaccggat gtttaaattc cagcaatatt ttcaataaac ttcttgtaat
     1081 ttctaatttg cgttcaattg gttgatagca atctgtatta cccgaaagca taatgggtga
     1141 tgcagtatgg ctttttttgt tcagctcttt tttgagtaaa tcgggtgctg tttttttcac
     1201 taaaataatc cgttcaaaat ccaagcccgc gctatatccc cagtagggat gcgtgtttcg
     1261 ggcatagcaa taagtacagc catgctcaca gccttcatac ggattcatgg aataatccat
     1321 aggaatatcc ggactgctta ttttgtttat gaatgttttg ggaaagactt ctatatattt
     1381 cgtggagtgg ttttcatcgg tttgttcatc ggcttccgat ttatcgtaga tatacgatga
     1441 atatctatgt tcactattgc tttgagctcc acgaccttta atatgatttt tatttatttt
     1501 cacatattga aaatgaatgc aataaaggaa tataaaaaag gagtttgttt tattctgcgg
     1561 tatcagattc taaagaagcc tgcgaaaccc aaaaattatt gtatccttca cccaaaacca
     1621 aaaacaacat tttttgttgt atcaattcca gcatagacaa taagataaat actgcatgta
     1681 ttttgctttc gcatgcctga aataattcgc tgaagttagc tctttctttc agctggataa
     1741 atgaaaccaa tttgcttttt tcttcttcta tagaatattt gaatggcgca atggtatgta
     1801 ctactttttc ggtgcggttt ttaaatttct ccatggagcg catatacgct ttcaaaagtt
     1861 taaacaagga taccgtttcc aaatctgcat cttcagccat taatttcacc aattggtcaa
     1921 cttcattttt ctggttgcca cgggcatatc gcaaagagcg actgccttcc atatctttca
     1981 aaacttctac aacatcttta aagcgtttgt actccaaaag ttgttgtact aattcctgac
     2041 gcgggtctat ttcattaccc tgatcatcca actcttttcg aggcaacaac atttttgctt
     2101 ttatgcgcat caatgtggct gctactaaaa taaattcgct ggcaatttca atatttaatc
     2161 tttctaaatg ttgaatataa tctaaaaaat cattagttat cttataaata ggaatatcat
     2221 gtatatttaa ctcatcgcgt tcaataaaaa acaacaataa atcgaaaggt ccttcaaatt
     2281 gcggtaattg tataataaat ggtgcactca tccttaaaac tctgtatttt acagaactaa
     2341 aaatagacat tttatttcgc gataccgatg atagttatct acacaccaaa agttaccaac
     2401 aggataaaat acgtattcga ttttgttttt gaacagtatt ttggtatgac ttattttgtt
     2461 accgaaaata aagatgaaag cataaatgaa gatgatattt tgttggtata tgccggaata
     2521 aatccagttt ctggatttag tatttttcag gataatttat tgttggaaga aacgattgca
     2581 gaacaacgca tttatgtttc acgtgaaaac aatttgccgg tattttttca aaccgacgaa
     2641 catttcaata tttcgtttga tattttttca tgcatttttt atttaatcac ccgctacgaa
     2701 gaatacttac cgcatactac ggatgcacat ggtcgctatc tgtcatctaa ttctatttta
     2761 gctaaaaaag aattttcttt tcgaccgata gtagaggagt ggttgtttta ttttaaagaa
     2821 gagttaatcc ggcattttcc tcagctagaa attaaagaac atacattccg gtatattccc
     2881 acttttgata tagaccaagc atatcgtttt ttgggcagaa actggcttaa gcatccaccc
     2941 aacatttttg ccaaagatgc actggctgtt ttattccaaa aaaagcaaga tccttatgat
     3001 gtgtatgatt ttatattccg tgagctggaa tcgtataact tacagcctgt tttctttttt
     3061 ctgttgaatg atgaaggagt aaacaacagc aaagtatcac caaaaagcaa aaaacttgaa
     3121 caactgattc ttcaggttgc ttctgcaaat tacccaaccg gaattcatcc tgcttatgat
     3181 gccttgaaag aattgacctt gttgcaagaa gctgaacttt tgaccgctat aaccggaaat
     3241 aaaacaaaca ttgcgcgtca gcatttttta aacatacgtt ttccagatta ctataaggaa
     3301 ttggttaaag cagaaataac tgacgactac tctatagcct atccagatgt gattggttgc
     3361 agagccggta ctacacaggc atttccattt tacaatttga ctgaaaataa agtttacaac
     3421 cttcagattc atccagcggt ctggatggat gctacatttg cctattatca gacagagaaa
     3481 aaaaatgagc aaataatgaa agatttttca gattttgttt tacatttaag gaaaattaac
     3541 ggtatattag taacaatata tcacaacgat ttattagcaa cggataaata cagatattta
     3601 tttcaaacca ttaaccaaca agtgaataaa ttttgaaaag tatgaaaaaa ttatttttta
     3661 cttttttatt aagtactttt tttataagta tttttaacaa ttgttttgcg caggactaca
     3721 agtactcaca gttttacaat tcgccactca acctgaatcc ttcttttacc ggacagattc
     3781 actctttata cagactggtg gccaattata gattacagta tcttcctata caaaaaccgg
     3841 caccctataa cacgttttcg ttttccggag atgtgggttt gcttcgcgac cgtctgaatg
     3901 gtgatatttt gggtgttggt gcagtattta ctaatgaccg ccagtcgtcg ataaaaagta
     3961 atttgatgat ggtgtctctt gcttatcaaa aaatattgac tcaaaagaaa aactcttatc
     4021 attacctgag tgcgggcttt caattcgggt ttactcaacg cagaattgat gtaaacaact
     4081 tagcctttga atctcagttt acaggtaccg gttttgatct cactttagat aacaaggaaa
     4141 acttctttaa agataaatac gttaagccga atatgaatat cggtttgatg tggacaggtc
     4201 gtttcaataa aatgataggt gcttatttag gattttccat gtttaatatc attaaagcaa
     4261 aagatacctt ttttgcttca gataatgagc gtgcttaccg catgaatgca catgccggat
     4321 tgaatgtaac catcaaagat tttgttctga tttctcctaa tatgatgtat atgcgtcagg
     4381 caaaagcaca acaaattatc ggcggtactt ctgtagcatt tagtttgaat aaagatgaac
     4441 ctttccgtac tgctgtttct gccggagttt ggtatgatgg aaatcaagct gtaattgcat
     4501 ctgcaggcgt ttccttttcg ggagtacaaa tcggattgag ctatgatgct actttcagaa
     4561 aagacttaag caaagcagac aaatctttcg gagctttgga agcatctatt atttatacca
     4621 gcaaaccatt ggaaaagaaa aaacgctatt ctccattact ttgtcctaat ttttaaaaaa
     4681 tacattccat taaaataaaa taaagcaatc cacacgggtt gcttttttta tatccaaatt
     4741 tttagctgag ctgatactca taagcttatt caggttaagt attttatctg gcgtaacact
     4801 actttttttg aactcaggag ttttatgtat taacctgagt tcgggataag cgtttcatta
     4861 aaaaaatagt tgatttttta tatagaacca cctcctttta attcctcctc catgtttagt
     4921 tggaggaaaa aatgagaaat caactatttt tttaccttaa ttttcttaac ccgaactcag
     4981 tttattaaca gattaccagc aaggagatat tagattattg tcttgttaga ttttaataat
     5041 aatttttacc acatagtttg catagatttt ttagaattaa tagtgctata ctactgtgtg
     5101 gtccaaacca acaaaggatt agattgtttt agcattgaaa tatcccaata atcttctttt
     5161 agtaaaaata atgtttcacg tgaaacatca ctatccacag actttatatt cacacgttta
     5221 taaaaatgtg agttaactac aatattgacc taagaaaaag aaaaaacttg tttcacgtgg
     5281 aacaaatcaa cacaaaataa acaatcactt tatgaacatg tgcaagaaaa tgtgaaatat
     5341 agaaatattt tacaaagtat taccggtttg gagcaatagc atttttattg aatagactaa
     5401 taaatacgtt tggtattatc tctatctttg tagcatgttc gcagaatatg atgtaatagt
     5461 agtaggtgct ggtcatgccg gatgcgaagc tgcagtagct gctgctaaaa tgggtagtaa
     5521 agtattgcta gccaccatga atatgcaaac catagcgcaa atgtcatgca accctgccat
     5581 gggtggagtg gcaaaaggac aaataatacg agaaattgat gccatgggtg gctattcagg
     5641 aatcgtatct gataagacaa tgatacaatt cagaatgctg aatttatcaa aaggcccagc
     5701 catgtggagt cccagaagtc aaaacgaccg tatgcaattt catttggtat ggagagaaat
     5761 gctggaaaat acaccaaaca tcgatttctg gcaagatatg gtgtttgaaa tcttagaaga
     5821 aaatggaaaa gcatgtggtg taaaaaccag cttaggacta gaaatcagag ctaaagccgt
     5881 agtattaacc aacggtactt ttctgaatgg tatcatccat atcggtgaaa gacaattagg
     5941 tggcggcagg gcaggagaga aatcttctaa aggtattacc gaacaattag taagcctggg
     6001 ctttgaaagt ggtagaatga aaaccggaac gcctccacgg attgacggac gctcattaga
     6061 ctataccaaa atggaggtgc aaaatggtga tgaccaaatt gtcggttttt cttacaaaaa
     6121 catagagaaa cccaaagagc aacgttgctg ctggattact tataccaatg atgatgtaca
     6181 tgaaatactg aaaaccggat ttgaaaaatc tcctatgttt cagggcagaa ttcagggttt
     6241 gggtcctcgt tattgccctt ccatagaaga taaaatcaat cggtttgccg acaaaaaccg
     6301 acatcagata tttgtagaac ccgaaggatg gaatacggta gagatatacg taaacgggtt
     6361 ttcaacttca ttacccgaag atgtacaata taaagcgctg aaaatgatac cgggatttga
     6421 acaggtaaaa atgtttcgtc cgggctatgc catagaatat gattattttc cgccaacaca
     6481 attaaagcgt tctttagaaa caaaattgat aaaaaatctg ttttttgccg ggcaaataaa
     6541 tggaacaaca ggttatgaag aagcagcagc acaagggatg atggctggaa taaatgctca
     6601 tttagtcgta aatgaaaaag atccgttgat attgcaaagg gatgaagcct atattggtgt
     6661 gttgatagat gacttagtaa gtaaaggtac agaagaacct taccgcatgt ttacctccag
     6721 agctgaatat agaattttat tgcggcaaga taatgctgat ttgcgcctaa ctgagttatc
     6781 gtataagctg ggatttgctg aagcagaacg gatgaaacgt gtagaagaaa agcaaaaagc
     6841 aaccaatgaa attatagact tattgaaaaa cagcagtatt gaacctcagg aaatagacaa
     6901 ctatttagtg gaaaaaggca caacagtttt gccccaaaaa atgaagttgg aaaagattgt
     6961 acttcgccca caaatcggtt tattggatat gacagaacat gtggctgctg taaaaacttt
     7021 gctcggcaag tatgaagatg agtttttgga acaagcagag ataagtgtaa agtatgaagg
     7081 ctatatagaa aaagaaaaag aactggtgcg taaaatgcaa aaagtagaga aactgaagct
     7141 aaaacccgat tttgattata acagcatacc gtcattaagt attgaagcca ggcaaaaact
     7201 gaataaaatt aagccggaaa ctgttgggca agccgggaga atatccggag tgagcccggc
     7261 agatatagca gtattgatgg tatacctgag taagtaaaac aaatttggaa atttgaaaaa
     7321 gccggataaa ataaacccat gtaaaaagat aattatacca attacctcat tatttaattg
     7381 tctaattatc tcgtttatac tttcatcttg tgctcagata tccgcaccta ccggaggtga
     7441 aagagataaa aatccgccga aaataaaagc agaaaaccca cctaataaat ctttaaacta
     7501 cagcggaaaa gaaattaaaa tctcttttaa cgagtggtta caaccattaa cgaatatcaa
     7561 aagtcaagtc attatttctc caaatattga tccttttcct aaaatcagta ttgaaagaaa
     7621 catccttcat ataaaattta aggataatgt actcctgccc aatactacat atagtctttt
     7681 ttttggtgat aatattaaag acaacaatga aggaaatgct tttcctaatt ttaaatacct
     7741 gttttcaaca ggaagctata ttgactcact tcagataaaa ggcaaaataa taccggctga
     7801 tggaaagcta gaagacaata cttatctggt tatttattct gatttatctg atacggcttt
     7861 tacaaaaacc cgacctatgt atgctaccaa aatagataac aatggtagtt tttctctgga
     7921 aaacgtaaaa gaaggacgtt ataaaatata caccttgact gataaaaatg cgaactacta
     7981 ctatgattta ccaacagaaa aaataggttt taccgattct gctctagtac tgaatgcaaa
     8041 tttagataca ttaagtattc accatttcct tcctgaagca gaaaaataca gaatagtcag
     8101 ctctgagaat actataaatg gaggtatgat aaaactagaa ttaaataaac cttacgctgt
     8161 tgaaaaggat gaatggaagg tttcattaaa agaagacagt acattaactg ctttatcatt
     8221 ccctcaagaa gatggaaaaa aactgcttgt ttattttcct aatctaaaaa gtgatacggg
     8281 cagataccag ttgatgctgt ttttgaacaa taatttggtt gacagcttgc ctgtaagaat
     8341 tgcatctgct aaatggaaaa agcctcaact attttttacc gataccagtg cttacaaaaa
     8401 tttaaaagtt tttgaaggta aaaagctgaa attgaagtca acaaattata cgacatctaa
     8461 accagacaca tcaaaaattt cattgacaga tagcgccggg caaaaacttt cttacgaaat
     8521 tcgtgttttg gaagatttat caagtttaga actaaaaacg gactggaaaa atggagtacc
     8581 gtacaaactg accataaatg actcagcaat gattgattta ttgggaaatt acaacaaaaa
     8641 gcaagatttt tctattttgg gcgtatcacc aaaaaaaggc ggtaacttat tgattaacat
     8701 agtattacct gattttaaga acgattacat cgtcattctt tctgataatt cgggtaaagt
     8761 atgggataga cgaattttac gtgattctca ggcgattaaa atggactatg gattactgcc
     8821 tgcgggtagt tactccttaa aaataattga tgataaaaac aataatggta ttgaaaactc
     8881 cggaagctat atgcacaaaa ccttgccgga aaaatcattc acctcaaaac cgcttaccat
     8941 aaaagaaaac tgggatgctg aagaaacggt gaaagcagac ttcagtatgc caacaattgt
     9001 tccacgtaaa acgaataacg aagatggggg ggacggtgta attgaaaaaa gtgaatttaa
     9061 aaatgagatt aaaaacattc cgggtaaaaa ggacatcgga aaaaattagt gtaaggaact
     9121 aattgtcatt aagagaaaac ttatagcttt ttcaaaattt aaggcaatat attcaactcc
     9181 aatatttttt atatttgaat aataaagttc taatttgtta aaataatttt tttctaactg
     9241 aatacccatc gctttctctc ctaatttttc ctttaaaaac catttttcta aaagtcgtgc
     9301 tgttctgccg tttccatctt gaaatggatg aatttttaca aacgctaaat gtatgtatgc
     9361 agcataataa aaaacctctt taaaatctaa gggttcgttt aataaaaatt gaatatcctc
     9421 aaatagtgca ataagttccg tttgtataat attcggtgtt gctgcaacat attcaatacc
     9481 tccatcttta tttaccacat acatgaggtt cgttctgatt tttccttgtt gatctgatgg
     9541 caataaatgt tgagaaataa gctgatgggt atgtataacg ttatcgtatg tgagattgtg
     9601 tgaaaaaata aattggtaag cagaaaataa atcatcagtc ttttctgtat agttttgttg
     9661 aaaagaaaca tttaagaatt tatgtttaaa aaaactatct aattctatgg cttctccttc
     9721 aattttagaa gaataaacag aagatatagc gttgtaaaaa ttataatagt ctatttgtaa
     9781 tggagtttct tcagcaatat taatatcatc aagagaatta atctctacgg atgcacaaaa
     9841 tgacggaagc aaattatctg aaagaatttt caagtagtta aaattattac ttcttaaaca
     9901 taaacaatac tgccataaat ataaacataa aactaattgc gtaattccat ttgaattttt
     9961 ctcccaaata aaaatacgca aacaacacaa atacaaacag cgatatcact tcttgtatca
    10021 gtttgagttg gtacgccgaa aatgtacgaa agccaatcct gtttgccggc accataaaac
    10081 aatactcaaa aaaggcaatg ccccagctta ataaaatggc atgtgtcatt ttcaaattga
    10141 cattctttaa attgccatac caggcaaatg ccataaaggt attcgagata aataataaga
    10201 gtattgtttt catcaagcaa agttcagtat ttttagtgaa caagaccaat attttgtatt
    10261 tttgaaactt tactgaccgt atgcgaaatt atctactctt tatacttgcc tttgttttag
    10321 tgtcttgcgg tgatgcttca aaacgtgaat tcaaactcac tccggcaaaa ggcggtgtct
    10381 attacggcgg cacattcagg tataatgaag aagaatactt caaaacactc tatccgctga
    10441 atattacgga agtaactgct catcgtatta tcgaacaaat ctatgaagga ctggtcacgt
    10501 ttgatgacag tactctggct gtcataccca acctggctaa atactggaca atagatgaaa
    10561 gtaaaacaaa atatacattt atattaaata ataatgtgta ttttcaagat gatgcttgtt
    10621 tccctgatgg aaagggcaga aaaatgacag cacaggatgt aaaatattgt ttcgacagat
    10681 tgtgctatca tcaccctgca gacaatcaag gtttctggat ttttaaagat gtagtgaaag
    10741 gagcgaatga atacgattcg ttaaccgaac aaaaaataat tcccaaagat ggtgtaagtg
    10801 gtgtgcgtgt tattaatgat actactttag agattgaact gtataaacct ttttcagttt
    10861 ttttacctcg cttgggcttg gcttttgcaa aagtatatcc acacgaagcc atagaaaaat
    10921 acggcgagtt gatagaacat gcagttggta ccggtccgtt ctatctcaaa gtagttcgtg
    10981 caaatcaggc tgttttttta gagcgcaaca aaaaatactg gaagaaagac gaatatggaa
    11041 accaacttcc gtatttgaat gccatacgtg tttcttttat aaaagacaag aaaaatgaat
    11101 tgctggaatt tcaaaaaggg caaagtgatt tagtttataa gtttcccttg gaaatgattg
    11161 gggaaatagt aaatcctgat aataaactgc aagctgcttt tgcaaaattt cagttgcaat
    11221 atatgcctac tatgtccttg cagtattatg gttttcagca tcagggtaaa atttttaata
    11281 atatcaattt acgaaaagca ttttgttatg ctgttgacag agaaagactc tgcaaataca
    11341 ccttaaaagg aaccggattt cctgctatat acggatctat accaccgggt acaggcacct
    11401 acgatgctaa acaagtaaaa ggatatcatt ttaatccggt tttggcaaaa gaatatttag
    11461 caaaagccgg ttatccgaat ggtagaggat tgcaaaaaat aatattagaa ctaaacagcg
    11521 gtggcggcag aaatatatct gtggcggaag ccattaaaaa aatgctggaa gaaaatctga
    11581 atataaaaat ggaattgctg atgattccct gggcacaaca cactgaagct gtagagagtg
    11641 ctaaggctga tttttatcgt ttaggctggg tagcagatta tcctgatggt gagaattttc
    11701 tcaatctttt tcacagcaaa tatgtacctg ctgatataaa tacaaaaaca agcataaatt
    11761 cattcagata tataaataaa aagtatgatg agtatttgga caaggcttta aactgtgtag
    11821 atccaaaaga aataaatgag tattatataa aagcagatca acaaatgata gatgatgcag
    11881 taatgttgcc tatttactac gatattgatt atcgtttgtt gcaaccttat gtgcgcaact
    11941 gtccacaaaa tgccatggag tttcgcgatt tcagcgaaat gtattttatc ccatttcaaa
    12001 cttcaacaca aaaataaata tgtcgtccgt agaacaacaa gccatagaaa aaagatatcg
    12061 cttcagaaat atattttctg ctttgccaca gtttgccaaa gacagtatct acggacttgt
    12121 acacagttat cgctattcta aatggaataa agccggaaac cctgtgccac cgccgcatgt
    12181 tgtaaaacaa gatgccataa aaagttatgg ctcaaaatat aatgtaaaga cctttataga
    12241 aacgggtaca tttttgggcg atatgctcga agccgtacaa aacaattttg ataccttata
    12301 cagcattgaa attgtagatt ttatacatga gctggcaaaa aacaggttta ggaacaatac
    12361 aaaaataaaa ttgctgctgg gtgacagctc tgtaaaactt ccggaattag ttcaacaact
    12421 aaaagtacct gctttgtttt ggttagatgg tcatttttcc ggtggagata caggatttgg
    12481 agatacaggt tgccccattt atgccgagat agatactatt tttgcatcac cgcacaagca
    12541 tattttattg atagatgatg cccgttgttt tattggtgaa gtaggctatc cgccattgga
    12601 agaattcaga aaatatgtat tgagtaaaaa gcccgaagca cagtttgaag taaaagatga
    12661 tattattcgg gtggtgtatt aggtatttaa tctatcaggg ttacaaatga agtttctttt
    12721 atacatatcg gtatttatct tattatctgg ttttattaag caatcggtat atgttgatcc
    12781 aacaggaacc tatattttga ataataaaac tgaatatgct aactcaacta aaattgggaa
    12841 aattcaagta aaaaaatttt ctgacaaaaa gataattctc aatttctata taaatatggg
    12901 atctcccgca tttaattctg gttctttttt agacacatta agttatgaaa aaaacaaagc
    12961 tgaatataag tgtcctttag aagatacatc ttgtaaagta accttgtatt tttataaaac
    13021 gggtataaaa gttatacaag aaaccgaaaa taaaaactcc ggatgtggtt ttggacacgg
    13081 tgttatagct agtgggtttt ataaaaggtt ttctacaaaa attccaatat ttgaagaccc
    13141 actaaaagaa aaataatata atttttgatt ttgagataat ggatagcccg aatcaagaac
    13201 ttattatccc agtcctctta tgcgcaatcg cagggcatct tttaaatcat cgccacagct
    13261 gcggcgttga actttttgag aaatgagttt tttgtagttg aactgctctt gataatacgc
    13321 ctgacaggtg gcacattttt caatctcttc tctaagctgg ttttctttat gcctgtctaa
    13381 ttcgccatcc agcataagct gaatgtcctt aagacaattg gtgcagatat gagttactgg
    13441 tcgcataaaa aagtaatttg taattagaca aaaaatatat acgagttatt atttaaaatt
    13501 ttattagatt gaattaagaa ttcaacattc atcattatcc tcaaatccca tctgatcggc
    13561 atagtctttc agtaattgtt tgagcatatt gcgggcacgg tgtaatcttg agcgaacagt
    13621 tccgataggg atatcaatta tttttgcaat ctcttcataa gagaaccctt caatatcgca
    13681 caacaaaatc accgtcttaa attcaatggg caacttattc agcgcattcg taatctcatc
    13741 acccatcata ttgtcaaaca aatcttcgcg taaatcaaaa taagccgcac tgttatcatc
    13801 atcttcgtcc gaaaaggtaa tcacatcatc ataatctatc ttttcgggct cacgtttttt
    13861 acgtctgtac tcgttgatga atatgttctt caatatttta aacaaccatg ccttagaatt
    13921 agttccctcc tgataagaat caataaaacg gaaggctttc atataggttt cttgcaccaa
    13981 atcattggca tcatcatcgt tatagctcag ttggtaagca aaattataca acgcatcggc
    14041 atgaggcata aattctttct caaaaaggcg ttctttatgg ataagggatt ctgacacaac
    14101 ggtcataaag ttagaatatt tttaacggat aaacagtaaa tttgttgata aagttccgca
    14161 attaaacgta aaatcgacat atctgtattg ggcacctgct gtatagctgt ctggatagtc
    14221 ggtttatttt attccaaatt tttgcaaagt gtgggtacgt actgctttgg gctgcttttt
    14281 ttgtatgggg ttaaaaatat aaatacaaat tttcaattac taagcaagaa gcaaaagttt
    14341 ttgctgttaa gtttacctgt actatttttt gcatatttaa ttacactgtt ttggtcggct
    14401 gataaacaac agtttttaca ggtaataaaa aacaaaatac cattcgtact cgtaccatta
    14461 agcctaatat ccttacaact tttaaaaccc gggcaactgc aatttttgat gaattggttt
    14521 ttgctggctt tattaggtgc atgtatctgg acattttatc aaatcagttt acaagacttt
    14581 gatattttaa tgcgattatc tgttgggcaa agtgttccga cattggtcca tcatattgca
    14641 ttttcggtgt tattggtatt tggaatattg ttttgtttgc gactttatga aatgactgaa
    14701 aataaaacag caaaaggaat ctatttagct gcaggtattt tctttttttt agtcaatcat
    14761 ttaatgatag cacgaagtgg tttggtccta acttatctct ccttagtcat ttattttatt
    14821 tataaaaaaa tagaacaaaa gcaatacgtg aaactcgcat tgagcatatt tttactggtg
    14881 gtgatagcct taataaatgt ctataaaaat ccttatctga aaaataaagt agattataca
    14941 atgacaggaa aaaaggcttt tgaaaatcaa gatgactctt taaatcagta ttctgatacc
    15001 cgacggatta tttcattgca attaggttat aaaatatttc agcaacataa aatagttggt
    15061 gtaggtattg gcgatgtgaa acaagaaatg cagcaattat accaacaaaa atatccaaaa
    15121 tttgaagaag aggtgcaggc aaaaatacat agtcagtact tgtttatttt agctgcttgc
    15181 ggaagtatat taggcatttt gtgttttatt tcatttgttt atatggccta ttatttctta
    15241 aacgaaaata aaatcgtgtg gtttattttg atgctgaata tattgctcgt ttgtttgtgg
    15301 gaagcatttc tagaaaatca gttaggcact tccatgtttt taattgtatt tattttaggt
    15361 tatttaggca acaataaaca aataaagtcg gtttgattta tgttgaaaat agtacacatc
    15421 accatggcaa aaacctggcg cggcggcgaa cagcagttag cctgtttgat ggaaaataaa
    15481 aacctgaatc aagttgtaat cggattaaat aacagtgcct ttgaaaaata tgctgctgaa
    15541 aataacttgc aattttatgg aatacggaat cacctttcct tttttagtgt actgcaattt
    15601 ataaaaaaat taaaaccgga tatcatccat gtgcacgaca gcaaagcaca taccatggta
    15661 tattgttgtc aattaatagc taaagaaaaa tggaaaattg tggtgcacag acgagtgtta
    15721 ttttctccat cttcatcttg gtttactgat aaaaaatata acgacgacag cgtaaaaaaa
    15781 ataatttgta tcagtgaagc tgttcaacaa gtaatgcaaa aagtaataac agaaaaatcg
    15841 aagttgactg ttatttattc cggcgtggat ataacaaaat ttgagactaa acaggatgaa
    15901 aaaaataaac taaatagtac tttttataaa gcatatcaac tgccagatca tgcacaaatt
    15961 attggggtgg ctgcagcttt agaaaatgaa aaaaatatag aagaggcaat agccattttt
    16021 aaaaatgtga ttgaaaaaaa tgaaaatgct tgtttggtca ttgcaggtaa tggatcgcta
    16081 ttcaatacgt ataaacaact gtatgaaaat gacaaaatac gttttatagg tttccaaaaa
    16141 gacatgaaaa ctttttacaa cagtatatca acatttttat ttacttccaa aaaagaagga
    16201 ttaggtactg cagtactcga agccatgtct gcaggtgtac ctgttgtttg ccataatttt
    16261 gaaacagcaa aagaaatgat agaaaatggt aaaaacggat atatttacaa caacaaaaat
    16321 gaagctgcaa caataataaa cacatttcta acagacgaaa ataagcgttc agcaataagc
    16381 aaaaatggaa tagaaatgat tcaacaaaaa ttcgacaaag aaataatgat tagtaaaaca
    16441 gaagaaattt attacagtat agtaaacata caagcatgaa agtgggtttg ttttttggtt
    16501 catttaatcc tgtgcatata gggcatttat tggtggctac gcgcatgcgt gaagctgtaa
    16561 atttggatga aatctggttt gtgctgtctc ctcaaaatcc gtttaaacta aatgcggaat
    16621 tggctgatga acagcagcga ttagaaatgt tgaatttgag tataaacgat attgattatt
    16681 tcaaagtttg tactttagaa tttgaactgg ataaaccatc ttatacacat atcacgctga
    16741 aaactttaaa aaaacaatat ccggaaatag aatttaagct cattattggc gaagacaatg
    16801 tgaacaaatt tcaagagtgg aaagaagcaa aatggataag tgataatatt gaaatattag
    16861 tctatgcacg gaaatcatta agcgataaaa atgaaaatga atcgttattt aaaatgaaaa
    16921 aaatcaatta cggctatata gatatttcct ccaccgaaat aagacaacgg attaaagaaa
    16981 ataaactaat tgatttttat gtagaagaaa aagtgaaaca atatattatg ttccacaagt
    17041 tgtatcagtt ctaaaataca cttatacaca tgcctgtgaa tttcatccgg aatgcctaag
    17101 tttattacat tatttttcaa catacgtgtt tgtgtataaa tgcattcaaa cgtgaataca
    17161 agtagataaa aatgcacttt ttataagtgc tgttttgctg taaaaatatc tccaccaaca
    17221 agagcagttt tcctaaattt aaacacagat aatacacaag aaaaacaaaa ataaacagca
    17281 gtaaactatc cacaattcat gtgaacaaac accatccaca actgttgaat aaacaaaaac
    17341 taatgcaata aaatatttcc aaaccactga aaacaaagta cttaataaaa gtgcataaca
    17401 tgtgcataaa acgctgaaat aacatttatc attaaacacc tatcattaag cattaatttt
    17461 ttctcaacga atccacatcc ttaataaagt ataataaaaa attcttttaa atacttatta
    17521 ttttatttat gctgtcgcag ctgctttagt caatctcaaa aaaaaacgta attttgaatt
    17581 tcataatcga ccatcatgac aaacgaaaca gacatcaaag ataaattggc agaacttccg
    17641 gagcgcggat ttttggacgt gcaaatcgac ccgactgtgg atttgtttgc tgaaattgac
    17701 cgcatgaaaa aagaaaaaaa tgcagtgatt ttagcccatt attaccaaac cgcagacata
    17761 caagacatcg cagattttat cggtgacagc ttgcaacttg cacagcaagc cgaaaaaaca
    17821 acagccgaca tcatcttgtt tgcaggagtg cattttatgg cagaaacagc gaaaatcctc
    17881 aacccaacca agaaagtgat acttccggat ttgaatgccg ggtgtagttt ggcagaaagt
    17941 tgccctgcgg aagattttgc cgctttcaaa aaaaaatttc ccggcttcgc cgtggtttct
    18001 tatatcaact gcacggcaga aattaaagca ctcagcgatt atatcgttac ctcttccaat
    18061 gccgaaatta tagtcaactc cattcctgaa gaacaaggaa tcattttcgc tccggataaa
    18121 aacttagggg cgtatctggt gaaaaaactc aacagagata tggtgctttg ggatggtgct
    18181 tgtatggtac acgaaatttt ttcactggat aaaatcattg ctttgcagga aaaatatccg
    18241 gaagccaaac tgatagccca cccggaatgc gaagcagcat tgctcgatat ggcagactat
    18301 atcggttcta ccaccaactt gctgaaatat actatgatag atgactctca agaatatatt
    18361 gtagctaccg aaacgggaat attacataag atgatggaag catcacctaa taaaacattt
    18421 atccccgcac cgcccgaaaa caactgtgcc tgcaatgatt gcccgcacat gaaattaaac
    18481 acattggaaa aagtatattt gtgtttgaaa tatgaagtac ctgaattgca gatggaagaa
    18541 acattacgtg tggcagcaaa aattccgata gaccgcatgt tggacatttc ggcaaaagca
    18601 ggattaaaat aaatcattta tttttccatt tgtatatagc actaattccg ataaggaaac
    18661 aaattaaaag tattacaggc atccaccaaa aatgtgctaa aaattttggt ttcagtttga
    18721 tttcatcgtg gttgccgtac aaaattaagt gcgaccagaa aaaaggttga gcagctatgg
    18781 catctgcatt tttcaaataa tgcattttgg ctgattgcag tgcttcagtc ttgttttttc
    18841 ccgacttcag taagccataa aaatctgcca taagtttagc cgtagcctca tctgcggcat
    18901 cccaacgtgt agccagtaca ttagcagcac cggcatattc aaacgcccag gcaaaatttt
    18961 gcaacccttc gccttgttgc cattttccgg cattgctttt acaggcagcc agacacacaa
    19021 attgtgcggg agtttgtaat ttccatattt catccaaagc caaatactcg ccattgctga
    19081 gcatgagttt tgacataagc gggcgcaagg tatcatacaa taaatgcgta ccgaaatgta
    19141 tgacaccgaa tttaggtaaa ttccagacga ccgctttttt cgtagcatat tgccctgata
    19201 agatggatac gtcattcagt ttatttatgc tttctttttc taattgaaaa tttaaaaccg
    19261 ataaaccgga acctgaaaaa ttgggaaaaa tggccaagca attgctttct gtcgctgtat
    19321 tttttttatg aaaaaaggta ttggcagaaa aagaaaacac cgttgtgtgg tcttctaaaa
    19381 aataatgttg tccgtcttct gatagaatat caaaaggcag aaaagaaata ttgcccgaag
    19441 gctcaattgt aatatgttct aatagtgtaa cctgactaaa caagagttta aatatcgctg
    19501 ttcccttttg tttatgatat tttttctcag ctacagcggt attcaaaacc cctatcaagc
    19561 tgtcagtaaa tgctgtattg gcaagacgta tatttttgac actaaatttg tcgatggcat
    19621 acaaataata aaaatctccg gcagtataga aagaaaaata ttgttcattg gcagataagg
    19681 attgttgaat ttcttttatg gaaataatat gctcgtcaaa acgcaaagca aaatatttgg
    19741 gataggcctt accaattttt tgcaccaact ccgcatattg cttttgataa aaatacaagg
    19801 aatcatactg tccgttttgt tcaaaaaaat aaatctgctt tttgatgtcg tcatattgcg
    19861 tacgaaataa ttgagggatg gatttctccg tttcaaagtc ttgtaagcgg gaacgcaata
    19921 aattggcttt actgctttgt gcaaaaaacc aaacttcatt taaaaaccaa gtatcttgcc
    19981 gggggtaaaa ttgtgtaagc agatataatg ccgtttgatt cagttcatca attttgcgat
    20041 tgtagtacaa tttattttct tgagtatagt atgaattttt cacgttctgt gctaaaatca
    20101 aacaagattg taattgttta atttcatttt ttatgtcatc agcagaaggt ttatgtaact
    20161 caaatttttc actgtttatt ttaaaacgaa ttaactcaat ttgccagtag tcaggtttat
    20221 ttgttatgtt tccaaattta tcggtgcata taattttagc tttattaatg tagttttcgg
    20281 cttgtgctaa atgatgttga gccagttcgt taattgccat accgcgataa gcatctatcc
    20341 ggtaagaatt attttcaccc aaaatctgtg tgctgcaatc taaagatttt tgaaaatact
    20401 gccgactgat tttatagtgt tgaagcaaac ggtgcacttc acccaattca taataaatat
    20461 cgataagccg ataagaaaca ggattaagta ccaattccat atcttcagct tcttgcaaat
    20521 aaaataaagc actgtctaac aggtgcatat ttttatatac tttgctcagc tctagtgctg
    20581 ccagcgcttt ttggtaatgc gcatttttga atgtcttatg actaatgacg tatgccgttt
    20641 tcaattcatt tttggcatca atatattctt cttttaaagc gtaagtcgtg ccacaattca
    20701 aataagcata aaatgcctcc gtagcttgtt ttccataaat ggcttccgtt atttttttat
    20761 cttgattaaa atggtacaac gccgattcta catcatcttt attcataaac aaaccaccta
    20821 aagtggcatg cgcctctgca atgcgcgggt ggttttgtgg caagcgtttt tcatacaagt
    20881 acaaggcttc agtaaaatac ttttcggaac gattcaggtc attttgttca ccatatatca
    20941 aacctaaatt ataaagcacc tgagcatatc gagtatgctt gttgtcatat atccttgaat
    21001 agatttgtaa ggcttgtaaa taagcatgtt ctgcactatc caactccgaa tctatataat
    21061 aacagctggc taagccatta tatgcagtag cttgcagacg gtctgtggtc ttgggcagca
    21121 gggaataaaa tatgacttta gccgcatggg ttttattttg ccttaataag atgtcagctt
    21181 ccaataagcg agtttcattg ctcggtttt
//
LOCUS       DAOQII010000008        21046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1141_length_21046_cov_5.496022, whole genome shotgun sequence.
ACCESSION   DAOQII010000008 DAOQII010000000
VERSION     DAOQII010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21046
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_21046_cov_5.496022"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2656)
                     /locus_tag="PLF48_00870"
     CDS             complement(<1..2656)
                     /locus_tag="PLF48_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47900.1"
                     /translation="MQAIIGAGNVHYQENGKWKTILSNIFSNTTGRHTDLAFSAPYNQ
                     HKIYFPNSPGKSIITEIYGQEFSDWGTPALVWLDKKGKEIAEVQINSGCVGSPDNLEL
                     DYKEIFPFVDAKIINSTTSKELKYILTNRAILDNKPTDAVYLAFRENINFDKSWKLNT
                     LSAEKNIAEKLGVEQVAKYYFSQNGKAILEINNPIYHDLVSLGTCADPDFDNSKNNED
                     AYFEGSYLIKNEKKNSFTSYILIPLEWLSKPERKFPITIDPTTNYYPGGTFPTYTANR
                     SGNSGDWECFAGTYAGRTYQFDISYGWVDDSWPFSNPYMDGYASFDLSAIPDNAVINS
                     ATTYWYRYAGRTCGDAINLKHGMVQYNENLAESYDCNVDGNRSRNNNGYYSGTGKNGT
                     GWQSQAANTSDITNSLSGNKFTAGWAYNGGDDCCTFVCGGDDGDYHHIYGNQHSTNKP
                     YISIYYCVKPTITTQPVGQLLCKANTMNALSVVATGTGLTYQWQASNYTSCSAATSGS
                     WQNITGATSSSYTPPKIAGTRLYRVIVTADCPTSVSGYQVTSSCVVVTVNPMDGSVSG
                     TTTGHPYATGDNAPPIQTGICGNTILPSSSNVMSALLPGAIGAANNITTFSWSATGGS
                     FSPTTGTTTTWTAPATPGSYTITVTQQDGCADADATKTCPVIVGSPACDYIYVAETGG
                     ADAVGCGGPNNPCRTLTGSDGALNKVSATRNYIRMATGTYTESNIVDLQSNLIIEGRY
                     TVSGGLWSKSSNTGASTSISCSGIGDGTDATLQQHVMGFRSDNDDNWKLIDLNITTVA
                     ASGQTSVGNGKSNYGIYIANGSTGYEITRCNVTSGAATKGLGRTDGTGFDGTAGTAGS
                     TGGNGNNQSSNGCNSGCSSGAGGSGGGGG"
     gene            complement(2725..2931)
                     /locus_tag="PLF48_00875"
     CDS             complement(2725..2931)
                     /locus_tag="PLF48_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47901.1"
                     /translation="MRKNLLYIFLTFFYTTSLFAGSNNDVGNEKKNIVLNDKSFSIEE
                     NIRVNPDDQTVTKIIYGVGGHSRI"
     gene            3493..4152
                     /locus_tag="PLF48_00880"
     CDS             3493..4152
                     /locus_tag="PLF48_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF47902.1"
                     /translation="MHGQPILVRLSKTYLIFYKFRLLHFTGCIFVHQDMSLVDESIFM
                     GVNVWSPLCDTNEKNGALFLIPKSHRIFPTYRNATITNIYDKHYAVIKRYMQPVYMKA
                     GEAILFDNSILHYSPPNLSNEIRIATNIFVTHREAKITISYHDKEKNKIERFEEEDDF
                     FTSYTQFDNCENMLRPKIGKSIGFTDYNFPVLTPEILEARYGKQKQLGFFDKLKEHIF
                     L"
     gene            4149..5018
                     /locus_tag="PLF48_00885"
     CDS             4149..5018
                     /locus_tag="PLF48_00885"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF47903.1"
                     /translation="MIAPIFKDKALQNAFDEFGFVKIPLLTKQEIEKLISLFYTHHHN
                     LSEDNFSASSFLNEKQKKTEIKEAIYPIFEQHFEKIFTNYTYFGSSYLYKTSGKNSAV
                     SPHQDWTIVDEKQFVAINIWTPLIDTNADNGTLFVVPKSQAQKMFALRAPTIPFYFLK
                     YFDAVLRCGIPTNAVAGEAVILNQSLIHYSSANTSDKIRIAITSGIKSKGAPMLFHYR
                     NAHNEINRYTMPEDFLLDFADFGKDIYETPANALDAEKIAYNHPAISKIDFLEKFGNK
                     RLSFWDKIKEHFR"
     gene            complement(4941..5804)
                     /locus_tag="PLF48_00890"
     CDS             complement(4941..5804)
                     /locus_tag="PLF48_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF47904.1"
                     /translation="MSSALQKLLALQGFCIFRMLHEDDILKLNSYCNTLFDNHKQEFV
                     SSSHFLPYDESVRINNILHAILNKPFVEMFPYLQLLGGTLAAKYKSSSILKAHNDWSI
                     VDESHYNSYNLWIPLVDTNKENGTLGLIPGSHLWKQNLRGLNIPGSFEKYTDKLMKTG
                     YEPALQAGEVILYNHKLVHYSRPNTTAKPRHVAIIGAKDKDAELQVSFCLDGKNIETY
                     KAQEEDFYVFDAELIKQRNRLINTQTILQTNVSWTEIEVEFKTNLPEMFLNLIPERQS
                     FITKLLQKINL"
     gene            complement(5818..7635)
                     /locus_tag="PLF48_00895"
     CDS             complement(5818..7635)
                     /locus_tag="PLF48_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF47905.1"
                     /translation="MLQLKNSYLQQHLSVNGYFTIPFVNNEIIQQLKEIYQKNFDDTL
                     TPFYSTSFHPDVDLKKRINEEVLNLIQADVEQLFSDFTILGTSFLKKKSNQENPLPLH
                     QDWTVTDEEKYGSFTIWIPLQDTDEQNGAIRIIPGSHLIDTQLRAPSLPVSFEKDRAL
                     FDKHLQTLPLKAGEAFVFNQKLMHASFGNSSSTDRLALTIGLIPKNAPLFMLYYDKQA
                     RKVKRYSMPDDMFLRYPEIIDKPMLGQLEDEFTYHPASLSEKTFKEKLYQNRLKHSTM
                     ESLFKSEEHQQYFEENGYIKIDAIDKNDIQQLIDFLFQSGIKKETGYGFYVGMDHENK
                     QLVKEMMQRIEDIAMPKVAPYLKDYKLITASYVIKDPHPHGIVPPHQDWTFVEDETRH
                     CSVTCWIPLVDTNMENGCMGVIKGSNHFFKSVRPSPSPQVPSPLAKHMFSLFPYFELL
                     PMKAGEALIFDNRTFHASPPNITEQPRLAIGLSFTQKNAELRHYYCKPGTKDTLLKYK
                     IDPEFFTRYDNKSLSELYNKGGLIEGYEMMGEMPFVWEDLTKEEMKKRVLATGNVYNK
                     ELTDYMKGLFSKYMKDGIIQKVKGFISRINPMRLVRNNG"
     gene            complement(7662..8585)
                     /locus_tag="PLF48_00900"
     CDS             complement(7662..8585)
                     /locus_tag="PLF48_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF47906.1"
                     /translation="MRKVFKADNLEAEFQKNGYVIVPFLSEAEVDYLKEKYFETLNES
                     GGNINEDDVAIKDKISYDFTFINKNIAYKKLVFNIIREKFKPHYDIYLDNYEPIIANF
                     IRKRTNDGEVPLHQNWAFVDERKCTSVSIWCPLIDSNEANGTLEITPGSHKRFGEIRG
                     PMIPWELENIKHDIIDKHMVPMNVKAGQAVILDDSVVHYSNINTTEGLRLTIQLILIP
                     AEVNSIHYHMNPEKSKTKVEVLEVDHEFYMNFNPWKKPENVKVLKSFSYQPFDMSLSE
                     FQNGLFNMRIDDKDAPPRKYSVWDKLKSFLN"
     gene            8623..9513
                     /locus_tag="PLF48_00905"
     CDS             8623..9513
                     /locus_tag="PLF48_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF47907.1"
                     /translation="MFFFRNKVNKKNSAQHSLNVGKFYDKNYDAFHQVYGEIIQAFRT
                     NNTDNLLEYQLKQIDIKTCDRVLDAGCGVCGPARYFARNTGCTIDAISISEKQINIAN
                     QHITNENLHKHITAQVFDYHVSDKLFHKETFDKIYFLESFGHSTDKEKLLNAMWKVLK
                     PGGEIYIKDLFRRISKNWFIQRKIDSEITKINKAYHYEVTDLNKFVDMVREIGFVIVF
                     IKTIDIPLNDFENLSISNEFQELTGIAKIDNWEKYIFPVDFFEVKLYKPEYDIEKGKH
                     RYFLQNIYQLQMNHLNKEDL"
     gene            9529..10497
                     /locus_tag="PLF48_00910"
     CDS             9529..10497
                     /locus_tag="PLF48_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNF47908.1"
                     /translation="MISLYHRIFDKVQYKFSLIFTGIYVVIYMGNSWNTTVFFLLFLA
                     ILTAIGIAGIGYLLNDWNDREQDKIAGKSNIFLSANKLLIVVIPIAFCVLAILPWLYL
                     PNTRITFYLLSIEIILFIVYAFPPLRLKERPVAGIICDAIYAQVLPCFIAAYTFSLTG
                     TNIKLKLNFILVYFVWLFLAGLRNILLHQIDDYENDIKSNTHTFIIVHTPTFIYKSAF
                     KLLLFFELLTFCYTLSSISIVSVFVFIFYIFIIVILNNNLNEIKYINFIAKRLFNEFY
                     EIHLPIIILIIFTTVKFNYIYLLLFHILLFSPVYYDYAKKFIQKYF"
     gene            10574..11713
                     /gene="dnaX"
                     /locus_tag="PLF48_00915"
     CDS             10574..11713
                     /gene="dnaX"
                     /locus_tag="PLF48_00915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNF47909.1"
                     /translation="MENFIVSARKYRPAQFLDVVGQEQVSETLKKALLSGKLAQSFLF
                     CGPRGVGKTTCARILAKAINCENPNADLEPCNVCPSCTSFNQNASFNIFELDAASNNS
                     VDDIRQLVDQVRYAPQSGKYKVYIIDEVHMLSQGAFNAFLKTLEEPPSYAIFILATTE
                     KHKIIPTILSRCQIFDFKRISEKDIVKQLHKICDNEHVQAEESALHVIARKADGGMRD
                     ALSLFDKIVGFSGKTLNYQDVIQNLNILDYEYYFRFTEYFMEEDAGACLNAFNEIINN
                     GFDGEIFLQGLAEHIRNLLVCKDEKTADLLESAEDVKVRYNEQAAQIPQTFLLNALQI
                     INQCDIDYKTSKNKRLQIEVALLKICFLNTQIENTLSVLLGEKKN"
     gene            11743..12372
                     /locus_tag="PLF48_00920"
     CDS             11743..12372
                     /locus_tag="PLF48_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47910.1"
                     /translation="MNKKIPVKKSPENITAKTKTIPVETSIPGKHLVDLNDQPETKRT
                     IQNPAVVTSTPAEKKQKKELPTRSGIKSLFKDLQQTTVQQVEEKLDLTTVTVQLLWKE
                     FLKENREKLQNAFLSVADRQVPHWENEEIVFTETNNISLELLQLHKMDIVSYFTRKST
                     QPIVHLKFNLDRKQEVKRDYKTPKERLLIMIDENPAVRELIKKLDLNLD"
     gene            12404..12766
                     /locus_tag="PLF48_00925"
     CDS             12404..12766
                     /locus_tag="PLF48_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNF47911.1"
                     /translation="MNKKTVVIGASPKPDRYSYKAVKMLEQHGHDVIACGFEPNFIGD
                     IPIVTEWKNYHQVDTVSLYLNPKRQKEYYEYILSLKPKRVIFNPGTENEELFILCKQH
                     HILPVEACTLVLLSTHQF"
     gene            12838..13404
                     /locus_tag="PLF48_00930"
     CDS             12838..13404
                     /locus_tag="PLF48_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47912.1"
                     /translation="MKKIILGLIVLMSISALTFAQKGSNPKSKSNAPKPKNYTKPVKV
                     TPEQKATRNADSLKVAMNLSPEQYEQVKKINLDYIQKKDALKAKYKEQVKQDSVKVKT
                     EVKDKYSPMGQDIKKLNQERVEKIKATVSEEQFKQWQAWKKSKKGNEKSKFEERPKGN
                     RQDYQKKSSEIKTKVHEKEEDFDDEEGL"
     gene            13582..14988
                     /locus_tag="PLF48_00935"
     CDS             13582..14988
                     /locus_tag="PLF48_00935"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNF47913.1"
                     /translation="MQSEFSKIKHLYNRFGFGLSTDIIKRASSLNAEINLLLNNNSVC
                     QKLETVSQEDVEKAKEIFKNNTDDKIKDLKNLLKSNVFELNSLWFQQMINNSNQLQEK
                     MSLFWHSNLACRSANPYFDQLYLDIIRQQALGNFGDLLKSVAKTPAMLQFLNGQQNRK
                     EHPNENFAREVMELFTLGRGNYSEQDVKEAARAFTGWGFDKTGNFKFRWQWHDAGMKT
                     IFGKSGSFNGDDVIDMLLANPQTAYHITEKIYRHFVNENGNATRVKQLSANFFSSNYD
                     IRKLLTEIIQSDWFYMAENMGNRIKTPVELLAGIFRIVPVVFESPDSLVFMERALNQI
                     LLNPPNVAGWKGGTAWIDSSSLLLRMKLPQYLFYDTSMNFTAKDIMPEMDSSTAYKSA
                     FVNQLAHTKLKGTPDWTNAEHVFSSIDKDSVAQYLLAVPLANTAKQILAKMEVSDAIN
                     PLHQRIIYIMSLPEYQLN"
     gene            14995..16218
                     /locus_tag="PLF48_00940"
     CDS             14995..16218
                     /locus_tag="PLF48_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNF47914.1"
                     /translation="MLFILNKNNMHYSRRNFLKTSGLLSASMALPQFLQAQNRQNNAP
                     GNDNILVVIQLSGGNDGLNMLVPYENDIYYNARPKIALKKQEIIPLKSSIGLHPALKD
                     FKKFYDAGNLTVLFDVGYPNPDRSHFRSMDIWHTASAAEDYLHTGWLGRYLDEKQNPD
                     AKLTRAFEIDDSLSLALKGENYKAMAFTNAKKLDAQIADPLFKLLHKAEEEHEGTAAY
                     LYKTFADTMQSADYIYQTVKPVSSAKTYPNSAFGKGMKTIVELIISGIETQVYYISLG
                     SFDTHNNQQKRQADLFEQLNNSLAVFYEDIKQQAGSRNVTTMIFSEFGRRVEENASMG
                     TDHGAANPVLLIGNNLKTEGYYNSPSDLKDLDEGDLKYRINFRDIYATVLNDYLKTDA
                     ARIIDKKDFVKMNLF"
     gene            complement(16210..16548)
                     /locus_tag="PLF48_00945"
     CDS             complement(16210..16548)
                     /locus_tag="PLF48_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47915.1"
                     /translation="MNTNKIAKYLFSLFFIIAIAVHVYYVIESDGKPLWWHVLYYITY
                     GVCWWAIFTRNKNRLILYCAMALFPFATHFYYAFRHLHPPDVMLAVCILVCVMLVSGF
                     WFIRFEEKSK"
     gene            complement(16577..17983)
                     /gene="lpdA"
                     /locus_tag="PLF48_00950"
     CDS             complement(16577..17983)
                     /gene="lpdA"
                     /locus_tag="PLF48_00950"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNF47916.1"
                     /translation="MNQFDVVIIGSGPGGYVCAIRCAQLGLNTALIERYATLGGTCLN
                     VGCIPSKALLDSSEHYHNALHSFKEHGIDLEQTPKVNFTQMIDRKAKVVATTCQGIDY
                     LMKKNKVAVFQGHGSFIDAHTISVLKEDGTTEQLLTKYVVIATGSKPSSLPFLPIDKT
                     RIITSTEALSLKTLPKSMIIIGGGVIGLELGSVYQRLGTQVSVIEFMDKIIPTMDEDL
                     SKELLRSLKKLGIKFYLSHKVTGAVNNGQQVTVTALDKAGKEIQLEAEYCMVAIGRRP
                     YTDNLGLENVGISLNERKQIPINEHLQTKVPTIYAIGDVIQGAMLAHKAEEEGVFVAE
                     TIAGQKPHINYNLIPGVVYTWPEVASVGQTEQQLKDKGIAYKVGSFPMRALGRARASM
                     DIDGLIKVLADAKTDEILGVHMIGARAADMIAEAVVAMEYRASAEDIARISHAHPTYT
                     EAFKEACLAATDNRALHV"
     gene            18087..18425
                     /locus_tag="PLF48_00955"
     CDS             18087..18425
                     /locus_tag="PLF48_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF47917.1"
                     /translation="MKKLIVLFGLFIMVFTLSAFKGKTATPFKNLQVLPDSTTEEQLD
                     AIMHAYNESLNVKCSHCHIKGNMASDENPEKEIARKMMRMTNEINEKYFGKDSGTVGC
                     MTCHNGKLHP"
     gene            complement(18431..19285)
                     /locus_tag="PLF48_00960"
     CDS             complement(18431..19285)
                     /locus_tag="PLF48_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47918.1"
                     /translation="MFSWLTKKIKSYIIKKASQHRAVDIVEVRTKEIGLLSVKTETVL
                     KLLNSFFLPITLLKITTDLVNRDGIKIGKMNYTKAVKLRARSETIITTNSEISSITSL
                     FQALTNLLAQPIYMRSIGKATLKFLWWTVDIDVDDVFEIHPSKLKIRKEETEEDRIVR
                     LQREEERKAKLAIVREEQKLKSAIRKEKRKEHYAARKEKILSRRHKENYIPKEERRRL
                     KEVSEESDAIIIEPIVWDENTVMEQMTFTKEEVVQLNNEDVAVNNEQLSINQDNEQKE
                     ESDKPSEE"
     gene            19378..20616
                     /locus_tag="PLF48_00965"
     CDS             19378..20616
                     /locus_tag="PLF48_00965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF47919.1"
                     /translation="MKENLTYLIAIPVFLFFVVLEYLAMRKKDPEYTRLNDGITNLNI
                     GVVHVLSKVIVGVFLLWVYGLVYDNIRIFTIPKGIISFIFCLIAYDFFFYWAHRFGHE
                     INLFWGAHGVHHQSEDYNLTVALRQSWFHAVMAFFIFLPIPFLGVDSKTFFSVLTINS
                     FYQFWIHTHLINRMPRWFEFVFNSPGHHRVHHGRNPKYIDKNHAGMFIIWDKLFGTFQ
                     EEEETPDYGITKPLNSWNPAWANIEYFSGMIQNARRFNFKDKLKYIFAKPGWQPDYAG
                     GTIAVNEVKPDYKKYNVRNSKGLNIYVVVQFLITIVGLVAYLQYYKQWSFFYQAINFI
                     LIVLSTVICGAILENKSWVKYAEFIRIAIILIDLNSLFYFNYVEFFEWCLLISIIAGV
                     YFIVWYALNINRIHLFRALK"
     gene            20645..>21046
                     /locus_tag="PLF48_00970"
     CDS             20645..>21046
                     /locus_tag="PLF48_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF47920.1"
                     /translation="MKFTFLGTGTSQGVPVITCNCEVCTSSDFRDNRTRTSLLIQSAT
                     TTVVIDTGPDFRAQLLREKVQDLDAVVFTHGHKDHVAGLDDIRPFNYMLHKTIKVYAE
                     LGVQDILKREFAYAFQPQEYPGVPQINLITID"
BASE COUNT         6709 a         3946 c         3596 g         6795 t
ORIGIN      
        1 ctccaccgcc accgccactg cctccagccc cactactaca gccactatta cagccattgc
       61 ttgattggtt attaccattt ccaccagttg accccgctgt ccctgctgtc ccatcaaaac
      121 ctgttccatc agtacgtcct aaaccttttg ttgctgcacc agaggtaaca ttacatctcg
      181 taatttcgta acctgtagaa ccattagcaa tatagatacc ataatttgat ttcccatttc
      241 ctacactggt ctgtccactt gcagcaactg tagttatgtt taagtcaatt aatttccaat
      301 tatcatcatt atcagatcta aaacccatca catgttgttg cagggtggca tctgttccat
      361 cacctatgcc actacaagaa atagaagtgc ttgcaccagt attagaagat tttgaccaca
      421 atccacctga aaccgtatag cgaccttcaa taattaaatt agattgcaaa tcaacaatat
      481 tagattcagt atatgtacct gtagccatcc ggatataatt tcgtgtggca cttaccttat
      541 ttagtgcacc atcagaacct gttaatgttc tacatggatt attaggtcct ccacaaccta
      601 ctgcatcagc tccccctgtt tctgctacat aaatataatc acaagcagga gaaccaacaa
      661 taacaggaca agttttggta gcatctgcat ctgcacagcc atcctgttga gttactgtaa
      721 tagtatagct accgggagta gctggagctg tccatgttgt agtggtaccc gttgtaggcg
      781 aaaatgatcc gcctgtggca gaccaactaa atgtagtaat attatttgca gcacctattg
      841 ccccaggtaa taatgcgctc atcacattag aagagcttgg taatatagtg tttccacaaa
      901 taccggtttg aattggaggg gcattatctc ctgtagcata cggatgtcct gttgttgtac
      961 cagaaacaga accatccata gggtttacag taactaccac acagctggaa gttacttgat
     1021 atcctgaaac actagtcggg caatcagcag ttacaattac acgatataag cgtgtacccg
     1081 caattttagg tggtgtatag gaagatgagg ttgcaccagt aatattttgc caactaccag
     1141 atgtagcagc actacaacta gtataattac ttgcctgcca ttgataagtt agaccagtac
     1201 ctgtagcaac tacagaaagg gcattcatcg tattcgcttt gcataatagc tgcccaaccg
     1261 gctgcgtagt tatcgttggt ttaacacagt aataaataga aatatatggt ttatttgtgg
     1321 aatgctggtt gccatagata tggtgatagt caccatcatc tccaccacaa acaaaagtac
     1381 aacagtcatc acctccgtta taagcccaac ctgctgtaaa tttgtttccg gacaatgaat
     1441 tggtaatatc gcttgtattt gccgcttggc tttgccaacc agttccattt ttacctgtac
     1501 ccgagtagta gccgttatta tttctacttc tatttccatc aacattacaa tcataagatt
     1561 ctgccagatt ttcattgtat tgcaccatgc catgtttcaa attgatggca tctccacaag
     1621 ttctgccggc atagcgatac caataagtgg tcgctgaatt tattactgca ttgtctggta
     1681 tcgcacttaa atcaaatgac gcatatccat ccatataagg attactgaaa ggccaagaat
     1741 catccaccca tccataacta atatcaaact gataggttct gccggcataa gtacctgcaa
     1801 aacattccca atctccgcta tttcctgatc tgtttgctgt ataggtagga aatgtaccgc
     1861 cgggataata attcgttgtt gggtctatcg ttatcgggaa ttttctttcc ggtttagaga
     1921 gccactctaa tggaataaga atatatgagg taaatgagtt tttcttttca tttttaatta
     1981 aataagaacc ctcaaaataa gcatcttcat tatttttaga attatcaaag tcgggatcag
     2041 cacaagtccc taaagaaact aaatcgtggt aaatgggatt attaatttct aatatagcct
     2101 tgccgttttg agaaaaataa tattttgcaa cttgttctac tcctaatttt tccgcgatat
     2161 ttttttcagc agacagtgta tttaatttcc atgatttgtc aaaattaatg ttttcgcgaa
     2221 aggccaaata cacggcatct gtaggtttat tatctaatat ggctctgttt gtcagtatat
     2281 atttgagttc tttactggtt gtagaattta taatttttgc atcaacaaaa ggaaatattt
     2341 ctttatagtc taactctaaa ttatcaggcg aacctacaca tccggaattt atttgaactt
     2401 ctgcaatttc tttacctttt ttatctaacc aaactaatgc aggcgtaccc caatccgaaa
     2461 attcttgacc atagatttcc gtaattattg acttacccgg tgaatttgga aaataaattt
     2521 tatgctggtt atacggtgct gaaaatgcca aatcagtatg tcttccggta gtattggaaa
     2581 agatatttga taaaatggtt ttccattttc cgttttcttg ataatgaaca ttacctgcac
     2641 ctatgatggc ttgcattgta ccgttctcag atctaaaata cttacctgtt gctgaacgca
     2701 acgaaatatc ttcgtcttct tgcttcagat tctggaatgg ccaccaactc cataaataat
     2761 ttttgtaacc gtttggtcgt cagggttaac tcgtatattt tcttctatag aaaatgattt
     2821 atcattaaga actatattct ttttctcgtt acctacatca ttatttgaac ctgcaaataa
     2881 tgatgtagta taaaaaaaag ttagaaaaat gtaaagtaaa ttttttctca taatcaataa
     2941 atttcatcga atttatgaaa tattttatca ttccgcaagt tttattataa aatatttgaa
     3001 tactgcctgt taagttacat aaatataatt aacacaaaat ctaagatttg taacatattt
     3061 ttatactatt tttaaagtta attacataat aatcaactaa ttacaataaa aaacatttaa
     3121 ataaaacttg gacaacatct tagatattca cgaaatttat tccgtgtatc ttattgaaga
     3181 ctaatttaat aaataatcaa attagtataa tatattagat tatacaataa atcaacattt
     3241 taatagattt ggagatattt ctgcgttaga tactatacat atcaagattc tactaatagc
     3301 aagaaaaaca caatctaagt gcgtaaacat gattttggaa cagcatcgaa gtaggaattt
     3361 tctgccgtag aaaatcgtat tattatttta tcaactatat ttgtagcttg aactcagcta
     3421 tttaaaatac tcctgaatta cataaaaaaa tccctgataa ttttttggat taccagggac
     3481 ttaattaata ttgtgcacgg acaacctatt ttagttcgat tgtctaaaac atatttaatc
     3541 ttttacaaat ttcgcttgtt gcactttacc ggatgcatct ttgtacatca ggatatgtct
     3601 ttagttgatg aaagtatatt tatgggtgta aatgtttggt cgccactctg cgatacgaat
     3661 gaaaaaaacg gggccttgtt tcttattccc aaaagtcatc gaatatttcc aacctatcgg
     3721 aacgcaacca tcacaaatat ttatgacaaa cattacgctg tcataaaaag atacatgcag
     3781 ccagtttata tgaaagcagg agaagctatc ttatttgaca acagtattct tcattattca
     3841 ccaccaaatt tatcaaacga aattagaata gcaactaata tctttgttac acacagagaa
     3901 gcaaaaatta cgattagtta tcacgacaaa gaaaaaaaca agatagaacg atttgaagaa
     3961 gaagatgatt tttttaccag ttatactcag tttgataatt gcgaaaacat gctccgccca
     4021 aaaataggta aatcaatagg gtttactgat tataattttc ctgtattaac accagaaata
     4081 ctagaagcta gatatgggaa gcaaaagcag ttgggtttct ttgataaatt aaaagaacac
     4141 atattcttat gatagcgccc atatttaaag acaaagcatt acaaaatgca tttgacgaat
     4201 tcgggtttgt taaaatacca ctgttaacaa aacaagaaat tgagaaatta atttcactct
     4261 tttacacgca ccaccacaat ctttccgaag ataattttag tgcttcttct tttcttaatg
     4321 aaaaacaaaa aaaaacagaa atcaaagaag ctatctatcc gatttttgaa cagcattttg
     4381 aaaaaatatt taccaattat acctatttcg gttcttctta tctgtataaa acatcaggta
     4441 aaaattcagc tgtgtcaccg catcaggatt ggacgattgt agatgaaaag cagttcgtag
     4501 ccattaatat ctggactccg cttattgata caaatgccga taacggtacg ttgtttgttg
     4561 tacccaaaag tcaggctcaa aagatgtttg ctttaagagc acctaccatc cctttctatt
     4621 ttctaaaata ttttgatgcg gtattaagat gtggtatacc gactaatgca gtagcaggag
     4681 aagcagtaat tctaaaccag agcctgatac attactcatc tgccaatacc tcagataaaa
     4741 tccgaatagc catcacatcc ggaataaaat caaaaggagc tcctatgtta tttcattatc
     4801 gaaatgcaca caatgagatc aaccgctata ctatgcctga agactttctg cttgactttg
     4861 ctgattttgg aaaagacatt tatgagacac cggctaatgc attagacgcc gaaaagattg
     4921 cttacaatca tcctgctatt tcaaagattg attttttgga gaagtttggt aataaacgat
     4981 tgtctttctg ggataagatt aaggaacatt tccggtaagt ttgttttaaa ctcaacttca
     5041 atttctgtcc acgaaacatt tgtctgcaat atagtttgtg tattaatcaa tctattgcgt
     5101 tgctttatca attctgcatc aaacacataa aaatcttctt cctgggcttt gtaggtttcg
     5161 atatttttcc catccaaaca aaatgaaacc tgtaattcgg cgtctttgtc ttttgcacct
     5221 attattgcaa catgacgtgg ctttgctgta gtatttggtc tggagtaatg cactagttta
     5281 tgattgtata atataacttc tccggcttgc aatgcgggct cgtagcctgt cttcattaat
     5341 ttgtctgtat atttttcaaa actgccgggt atatttaacc cgcgcaaatt ttgtttccat
     5401 aagtgtgaac ctgggatcaa acccagtgtg ccgttttctt tatttgtatc taccaatgga
     5461 atccataaat tatacgaatt ataatgcgac tcgtccacaa tactccagtc attatgtgct
     5521 ttcaaaatgc tgctactttt gtattttgct gcaagtgttc cgccaagcaa ttgtaagtag
     5581 ggaaacatct caacgaatgg cttattgaga atagcatgca agatattgtt aatgcgtacg
     5641 gattcatcat aaggcaaaaa atgcgaagaa gatacaaact cttgcttatg gttatcaaaa
     5701 agtgtattac aataagaatt taattttaat atatcatcct catgtagcat tctgaaaata
     5761 cagaagcctt gcaaggctaa tagcttttgt aaagctgagc tcatatctgg atttaaatta
     5821 tccattattt cgcactaagc gcatggggtt aattcttgaa ataaatcctt ttactttctg
     5881 tattatcccg tctttcatat atttactaaa caatcctttc atataatcag ttagctcttt
     5941 attatagaca tttcctgttg ccagcactct ttttttcatc tcttcttttg ttaaatcttc
     6001 ccagacaaag ggcatttctc ccatcatctc atagccttca ataagaccac ctttattata
     6061 caattccgaa agtgatttat tatcatatct tgtaaaaaac tcaggatcta ttttatactt
     6121 gagcaaggtg tcttttgtac ccggcttgca atagtaatgt cgcagttcag catttttctg
     6181 cgtaaagctc aacccaattg ccaatctggg ttgttctgtt atatttggcg gtgacgcatg
     6241 aaatgttcga ttgtcaaaga ttaatgcttc ccctgctttc ataggcaaca actcaaaata
     6301 tgggaaaagt gaaaacatgt gttttgctaa cggcgaaggc acttgtggcg aaggcgaagg
     6361 ccgtacactt ttaaagaaat gattacttcc tttaattacc cccatgcacc cattttccat
     6421 attggtatct accaagggta tccaacaagt aacggagcaa tgtcgggttt catcttctac
     6481 aaaagtccag tcttggtgag ggggcactat accatgcgga tgaggatctt ttattacata
     6541 acttgccgtg atgagcttat aatctttcag ataaggtgca actttcggca tggcaatatc
     6601 ttcaatgcgt tgcatcatct cttttacaag ttgtttattt tcatgatcca tgccaacata
     6661 aaacccgtag cctgtttcct tctttattcc tgattggaat aagaaatcaa ttaattgctg
     6721 aatgtcattt ttatctattg catctatttt aatataccca ttttcttcaa agtattgttg
     6781 atgctcttcg cttttaaata aagattccat agttgaatgt tttagccggt tttgatataa
     6841 tttctcttta aatgtctttt ctgaaaggga tgcaggatga taagtaaatt catcctccag
     6901 ctgtcccagc attggcttat ctataatttc aggataacgc aaaaacatat catctggcat
     6961 tgaataacgt ttcacctttc gtgcttgctt atcataatac agcataaaca atggtgcatt
     7021 tttaggaatc agtcctatcg ttaatgctaa tctgtcagta ctgcttgaat tgccgaatga
     7081 ggcatgcatc aacttttgat taaaaacaaa agcctctcct gcttttagtg gcagtgtttg
     7141 taaatgttta tcaaacaagg ccctgtcttt ctcaaatgaa acaggcaagc tgggtgctct
     7201 taattgcgtg tctataagat ggcttcccgg aataatgcga attgcaccgt tctgttcgtc
     7261 tgtatcttgc aagggaatcc aaatggtaaa tgaaccatat ttctcttcgt ctgttactgt
     7321 ccaatcctga tgcaagggca acgggttttc ttgattgctt ttctttttga gaaaggaagt
     7381 tcctaagata gtaaagtcag aaaataattg ctctacatcg gcttgtatca aattcaatac
     7441 ttcttcatta atccgctttt tcaagtctac atcaggatga aaggaagtgg aataaaatgg
     7501 agtcaatgtg tcatcaaaat tcttttgata aatctccttt agctgttgta taatttcatt
     7561 atttacaaaa ggaattgtaa aatatccgtt taccgaaagg tgttgttgta agtatgaatt
     7621 tttaagttga agcatgtaga ttttatttca tggcaatagt attaatttaa aaaggatttg
     7681 agtttatccc acaccgagta ttttctgggt ggtgcgtctt tatcatcaat acgcatattg
     7741 aacagtccgt tttgaaattc tgacaaactc atatcaaaag gttgatacga aaaggatttc
     7801 agcactttta cattttcggg ttttttccat ggattaaaat tcatgtaaaa ttcatggtca
     7861 acttctaaaa cttccacctt tgttttactt ttttctggat tcatatgata gtgtatagag
     7921 tttacttctg ccgggataag aattaattga atggtcagcc gtaatccttc tgttgtgtta
     7981 atattggaat aatgcaccac gctatcatct aaaattacag cctgccctgc ttttacattc
     8041 atgggcacca tgtgcttgtc aataatatcg tgtttaatat tttccaactc ccaaggtatc
     8101 atcggtccac gaatttcacc aaaacgctta tgcgaaccgg gagtaatttc taatgttccg
     8161 tttgcttcat tactgtctat cagaggacac cagatagaaa cactggtgca ttttctttcg
     8221 tcaacaaatg cccaattttg atgcaatggc acctcaccgt cattcgttct tttacgtata
     8281 aaattggcaa ttatgggttc ataattatcc aaataaatgt catagtgagg cttaaatttt
     8341 tctctaataa tattaaacac taactttttg taagcaatat ttttattgat gaaagtaaaa
     8401 tcatatgaaa ttttgtcttt aatagccaca tcatcttcat taatatttcc tccgctttca
     8461 ttcagggttt caaaatattt ttctttaaga taatcaactt cagcttcaga caagaaaggt
     8521 acaataacat aaccgttctt ctgaaattct gcttctaaat tatctgcttt aaaaactttt
     8581 ctcatggtga atttttagta ttttcaacat ctaaataaat acatgttctt ctttagaaat
     8641 aaagttaata aaaaaaacag tgcccaacat tcactaaacg taggtaaatt ctatgacaaa
     8701 aactatgatg cttttcatca ggtttatggt gaaatcatac aagcctttag aacaaacaat
     8761 acggacaacc tattagaata tcagcttaaa caaattgata ttaaaacctg cgacagagta
     8821 ctggatgcag gttgtggtgt atgtggtcct gccagatact ttgcacgcaa tacaggttgt
     8881 accatagatg ccatatccat atcagaaaaa caaattaaca ttgcgaatca gcatattaca
     8941 aatgagaatt tgcacaaaca tataactgca caggtatttg actatcatgt tagcgacaag
     9001 ttatttcata aagaaacttt tgataaaatt tattttttag aatctttcgg tcattcaaca
     9061 gataaagaga aattgctcaa tgctatgtgg aaagtgttaa aaccgggcgg agaaatttat
     9121 ataaaagact tattcaggcg aatatcaaaa aactggttta tccaacgcaa aatagacagt
     9181 gaaataacaa aaataaacaa ggcttaccat tacgaagtaa cagacttgaa caaatttgtt
     9241 gatatggtaa gagagatcgg gtttgtcatt gtttttatca aaacgattga tattcccctg
     9301 aacgattttg agaatctttc aatttccaac gagtttcaag agcttacggg tatagcaaaa
     9361 atagacaatt gggaaaaata tatttttcct gtcgattttt ttgaagtaaa gctatacaag
     9421 cctgaatatg atattgagaa aggcaaacat cgctattttc ttcaaaatat ttatcaactg
     9481 caaatgaatc atctgaataa agaagattta tagttgtatc ttaatttcat gatttcgcta
     9541 tatcatagaa tcttcgataa agtacaatat aaattcagcc tgatatttac aggtatttat
     9601 gttgtcattt atatgggcaa ttcgtggaat acgactgtat ttttcttatt attcttagca
     9661 atacttacag caattggcat tgccggaatt ggttatttat taaatgactg gaacgataga
     9721 gaacaagata aaatagccgg taaatcgaat atttttctgt ctgctaataa gttattaata
     9781 gtagtcattc cgattgcatt ttgtgtgttg gctatattac cctggttgta tttgccaaat
     9841 acaagaatta ccttttacct gttgagtatt gaaatcattt tatttatagt ctatgcattc
     9901 ccaccattgc ggctaaaaga aagaccagtt gcaggtatta tctgcgatgc aatctatgcg
     9961 caagttctgc cttgttttat cgctgcttat acattttctc taacaggaac aaacatcaaa
    10021 ctaaaactca atttcattct tgtttatttt gtatggctct tcttagccgg gctcagaaat
    10081 attctactcc atcaaatcga cgattatgaa aatgatatta aatccaatac tcatactttt
    10141 attatagttc atacacccac atttatctat aaatctgctt ttaagcttct gctttttttc
    10201 gaactgttga ctttctgtta tactctatct tctatttcaa tagtttcagt atttgttttc
    10261 attttctaca tttttataat agtaatatta aataacaatc taaatgaaat taaatacatc
    10321 aacttcattg caaaaagact atttaatgaa ttctatgaaa tacatttacc tattataata
    10381 ttaattattt tcactactgt aaagttcaat tatatttact tacttttatt ccatatattg
    10441 ttgttttctc cggtgtatta tgattatgca aaaaaattta ttcaaaagta tttttaaatc
    10501 aattgctatc attgttttca aaaaaatgat acagaattgt gcataacaat atccctttcc
    10561 ctatctttac cgcatggaaa attttatcgt ttcggctcgt aaatatcgcc ctgcccagtt
    10621 tttagatgtc gtggggcaag aacaagtgtc tgaaacgcta aaaaaagctt tgctttccgg
    10681 caaactggca cagtctttcc tgttttgtgg cccgcggggt gtgggcaaaa ccacttgtgc
    10741 acgcatcctg gcaaaagcca tcaactgcga gaaccccaac gctgacttag agccttgtaa
    10801 tgtctgccca tcttgcacct cttttaacca aaatgcatca tttaatattt tcgagttaga
    10861 tgccgcttcc aacaatagtg tggacgatat ccgccaattg gttgatcagg tgcgctatgc
    10921 tccgcaaagc ggcaaataca aagtctatat cattgacgag gtacacatgc tctctcaggg
    10981 tgcttttaat gcattcttga aaacactgga agagccacca agttatgcca tttttatctt
    11041 ggctactacg gaaaaacata agattatccc aaccatttta tcgcgttgcc agatttttga
    11101 ttttaaacgt atctccgaaa aagatatcgt gaaacaattg cataaaattt gtgacaatga
    11161 gcacgtgcag gctgaagaaa gtgctttaca tgtcatcgct cgcaaggctg acggtggtat
    11221 gcgcgatgct ttgagtttgt ttgataagat agtagggttt tcgggcaaaa cattgaatta
    11281 tcaggatgtt attcaaaacc tcaatattct cgactatgaa tattattttc gttttacgga
    11341 gtatttcatg gaagaagatg cgggtgcttg cctgaacgcc tttaatgaaa tcatcaataa
    11401 cgggtttgat ggtgaaatat ttttgcaggg actggcggag catatccgga acttgctggt
    11461 ttgtaaagac gaaaaaactg ccgacttatt agaaagtgct gaggatgtga aggtacgtta
    11521 taatgaacaa gctgcccaga ttcctcaaac atttttgtta aacgctttgc aaatcattaa
    11581 ccaatgtgat attgactata agacatcaaa aaacaaacgc ctgcagattg aggttgctct
    11641 acttaaaata tgtttcctca acacgcagat tgaaaacacc ttgagcgtat tgcttggcga
    11701 aaaaaaaaac tgaatgattt aattacatgg tttaaagaca ggttgaacaa gaaaattcct
    11761 gttaaaaaat cacctgaaaa tattaccgca aagacaaaaa ccatccctgt agaaacttct
    11821 atcccgggta aacatctcgt tgatttaaat gatcaacctg aaacaaaacg aacaatacaa
    11881 aaccctgcag ttgtcacgag caccccggct gagaagaaac aaaaaaaaga actacctact
    11941 cgctcaggca taaaatctct ttttaaagac ttgcagcaaa caacagttca gcaagttgaa
    12001 gaaaaactag atttaacaac tgttacggtg caattacttt ggaaagagtt tttaaaagaa
    12061 aacagggaga aattacaaaa tgcattttta agcgttgccg acaggcaagt gccacattgg
    12121 gagaacgaag aaattgtatt tacagaaacc aataatatct ccttagaatt attacagtta
    12181 cacaagatgg atatcgtttc ctatttcact cgaaaaagca cacaacctat tgttcatctg
    12241 aaatttaatt tagatagaaa acaggaagta aaacgcgact ataaaactcc gaaagaacga
    12301 ctgttgatca tgatagatga aaacccggca gtaagagaat taattaagaa actagacttg
    12361 aacttagact agcccatctg taaataatag attactttta aacatgaaca agaaaacagt
    12421 agtaataggt gccagtccaa aaccggacag gtatagctat aaagccgtga aaatgctgga
    12481 acaacacggg catgatgtga ttgcctgcgg attcgaaccc aattttattg gtgatattcc
    12541 catagttacg gaatggaaaa actatcatca ggtagacacc gtatctcttt acctaaaccc
    12601 gaaacgccaa aaagaatatt acgagtatat cctttctttg aaacccaagc gagtaatttt
    12661 taatcccggt acagaaaatg aggagttgtt tatattatgt aaacaacacc atatactccc
    12721 ggttgaggca tgtacactcg tgttactgag tacgcaccag ttttaacaat attttaagaa
    12781 gcttgaatta aacgttggca caattatttc atctaatctt tcaaatattt atcaatcatg
    12841 aagaaaatca tcttaggact catcgtatta atgagcattt cagccctgac atttgcacaa
    12901 aaaggcagta atccgaaatc taaaagcaat gcaccaaaac ctaaaaacta caccaaacct
    12961 gtaaaagtta caccggagca aaaagctact cgcaatgctg attctctgaa agttgcaatg
    13021 aatcttagcc ctgagcaata tgaacaagtt aaaaaaatca atttagatta tattcagaaa
    13081 aaagatgcac ttaaggctaa gtacaaagaa caagtaaaac aagattctgt aaaagttaaa
    13141 acagaggtta aagacaagta ttctccaatg ggtcaggata ttaaaaaatt gaatcaagag
    13201 cgagttgaaa aaataaaagc cactgtaagc gaagaacaat tcaaacaatg gcaagcctgg
    13261 aaaaaatcta aaaaaggaaa tgagaaatcc aaatttgaag aacgtcctaa aggtaacaga
    13321 caagactatc agaagaaatc aagtgaaata aaaactaaag ttcacgagaa agaagaagat
    13381 tttgacgacg aagagggctt ataatcttac tttaaaaaaa taaatataaa aaagaggcga
    13441 actttcgcct cttttttttg acctacgagg tgataaaaag catcgttatt ttacaagcat
    13501 cacaatatca attacttata aaatcgaact ataccaacct acgaggttaa actttctgct
    13561 tttattagag tctaattttg catgcaaagc gaattctcca aaataaaaca tctttataac
    13621 cgtttcggtt ttggattatc aacggatatt ataaaacgtg cctcttcttt aaacgcagaa
    13681 attaatctgt tgcttaataa taattccgtc tgtcagaaat tggaaaccgt atctcaagaa
    13741 gatgtagaaa aagccaaaga aattttcaaa aacaatacag acgacaaaat aaaagactta
    13801 aagaacttac tcaagtctaa tgtttttgag ttgaacagtt tgtggtttca gcaaatgata
    13861 aacaacagca accagcttca agaaaagatg agtctttttt ggcattccaa cttagcttgc
    13921 cgttctgcca atccctactt cgatcagctt tatctcgata tcatccggca acaagcgctt
    13981 ggaaattttg gcgatttatt aaagtctgta gccaaaactc ctgctatgct ccaatttcta
    14041 aacggacaac aaaaccgcaa ggaacatccc aacgaaaatt ttgcgagaga agtgatggaa
    14101 ttattcacgc ttggcagggg caattattcg gaacaagatg taaaggaagc agcaagagct
    14161 tttaccggtt ggggatttga caaaacaggt aatttcaaat ttcgctggca gtggcatgat
    14221 gcaggaatga aaactatatt tggtaagagt ggcagtttta acggtgatga tgtaatagat
    14281 atgctcttag ccaatccaca aacggcttac catattacag aaaaaatata caggcatttt
    14341 gtgaatgaaa atggcaacgc tacacgcgtc aaacaactgt ccgcaaattt ctttagttca
    14401 aattatgata tccgtaaact gctcaccgaa attattcagt ccgattggtt ttatatggct
    14461 gaaaacatgg gcaatcgcat caaaacacca gttgagttat tagcgggtat ttttcgtata
    14521 gttcctgttg tgtttgaaag tccggatagt cttgttttta tggaacgtgc actcaaccag
    14581 attttactca acccgccgaa tgtagccgga tggaaaggcg gcacagcctg gatagacagt
    14641 tccagcctgc tattgcggat gaaacttcca caatatttat tttatgatac gtctatgaat
    14701 tttactgcaa aagatatcat gcccgaaatg gatagcagta ctgcctataa atcggcattc
    14761 gtcaatcagt tagctcatac aaaactgaaa ggcactcccg actggacaaa tgcagagcat
    14821 gttttttctt caatagataa ggacagtgta gcgcaatatt tgctggctgt tccattagct
    14881 aatacggcaa aacaaatact ggcaaagatg gaagtgagtg atgcaataaa tccattacac
    14941 caacgcatta tttacatcat gtctttgccc gaatatcaat tgaattaatt tttaatgttg
    15001 tttatcctga ataaaaataa tatgcactat tcacgcagaa atttcctaaa aacatccgga
    15061 ttattgagtg cttcgatggc tctgccacaa tttctgcaag ctcaaaaccg ccaaaacaat
    15121 gcacccggaa atgacaatat tttagtggtc atacaacttt caggcggcaa tgacggatta
    15181 aatatgttag tgccttacga gaatgatatc tattataatg cccgtccaaa aattgcactc
    15241 aaaaagcaag aaattatccc cttaaaatca tcgataggtt tgcatcctgc attaaaagat
    15301 tttaaaaaat tttatgatgc aggaaatttg actgttttat ttgatgtggg ctatcccaat
    15361 cccgatcgtt cgcattttcg ctctatggat atctggcata ctgcatcagc tgctgaagat
    15421 tatttgcaca ccggatggtt agggcgttat ttagatgaaa aacaaaatcc ggatgcaaaa
    15481 cttacgcgtg catttgaaat tgacgactca ctcagcctgg ctttaaaagg tgaaaattat
    15541 aaagcaatgg cgtttactaa tgcaaaaaaa ttagatgcac agattgcaga tccgttattt
    15601 aaattattgc ataaagccga agaggaacat gagggaacgg ctgcttattt atacaaaaca
    15661 tttgccgaca cgatgcaaag cgcagattat atttatcaaa ccgtgaagcc tgtttcttct
    15721 gctaaaactt atcctaattc agcttttggc aaaggcatga aaaccattgt tgagttgatt
    15781 atttcaggta tagaaacgca ggtttattat atttcgctcg gcagttttga tacacataat
    15841 aatcaacaaa aaagacaagc ggatttattt gaacagctca ataacagttt agctgttttt
    15901 tatgaagaca taaaacaaca ggcaggcagt agaaatgtaa ccactatgat tttttctgaa
    15961 ttcggtcgca gagtagaaga aaatgccagt atgggtaccg accacggtgc agctaatccg
    16021 gtgttgttga ttggaaataa tctgaaaaca gaagggtact ataattcacc atctgatttg
    16081 aaagatttgg atgaaggtga tttgaaatat cgcatcaatt ttcgggatat ttatgccaca
    16141 gttttgaatg attatctgaa aactgatgcg gcaagaataa tcgataaaaa ggattttgtg
    16201 aagatgaatt tattttgatt tttcttcaaa tcggataaac caaaagcctg aaaccaacat
    16261 cacacaaacc aatatgcaaa cagccagcat cacatctgga ggatgaagat ggcggaaagc
    16321 ataataaaaa tgagtagcaa acgggaataa tgccattgca caatataaaa ttagccgatt
    16381 tttattgcgt gtaaaaatag cccaccagca aacaccatac gtgatataat ataaaacatg
    16441 ccaccataat ggcttgccat cagactcgat tacatagtat acatgtaccg cgatagcgat
    16501 tataaaaaaa agagagaaaa ggtattttgc tattttatta gtattcattc acgtacttac
    16561 tactttaaac taacacctac acatgcaaag ctctgttatc cgttgcagcc aaacaagctt
    16621 ctttaaaggc ttcggtatag gttggatgcg catgtgaaat acgggcgata tcttctgcac
    16681 tggctcgata ttccatggcc accacagctt ctgctatcat atcggcagcc cgtgcaccaa
    16741 tcatgtgcac gcccaaaatt tcatctgttt ttgcatccgc cagtactttt atcaagccat
    16801 caatatccat acttgcacgc gctctgccta atgcacgcat cgggaaagat cccaccttat
    16861 aagcaatgcc cttatcttta agttgttgct cggtttgtcc tacagatgcc acctccggcc
    16921 aggtatatac tactcctgga atcagattgt aattaatatg cggtttttgt ccggcaatag
    16981 tttctgccac gaatacacct tcttcctcag ccttatgtgc cagcattgcg ccttgtatca
    17041 catcgccaat agcataaata gttggcactt tcgtttgtaa atgttcattt atcggaattt
    17101 gttttctctc attcaaggaa atgccaacat tttccaagcc taaattatcg gtataaggtc
    17161 ttcttcctat ggcaaccatg caatattctg cttccaattg tatttctttt ccggctttat
    17221 ccaatgccgt aacggttact tgttgtccgt tgtttactgc tcccgttact ttatgcgata
    17281 agtaaaattt aatacccaat tttttcaggc ttcgcaataa ttcttttgac aaatcctcat
    17341 ccatagtcgg gatgatttta tccataaatt ctattacaga cacctgtgta cccaggcgtt
    17401 gatatacgga acctaattcc aacccgatta cacctccgcc aataatgatc atggatttgg
    17461 gcagtgtttt taatgataat gcctcggtag aagtaataat tcttgttttg tctataggta
    17521 aaaatggcaa ggatgaaggc ttagagcctg tagcaatgac tacatatttt gtgagcaatt
    17581 gctcggtcgt gccatcttct tttaaaacag aaatagtatg agcatcaata aatgaaccgt
    17641 gcccctgaaa tacggctact ttgttttttt tcatcagata atctataccc tggcaagtgg
    17701 tagcaactac cttagccttg cggtctatca tttgggtaaa atttactttt ggtgtttgtt
    17761 ccaaatctat accgtgttct ttgaagctat gtagtgcatt gtgataatgc tcggaagaat
    17821 cgagtaaagc cttagacgga atacaaccca cattgaggca tgtgccgccc aacgtagcat
    17881 agcgttctat aagagccgta tttaagccta attgcgcaca ccggatggca cacacatagc
    17941 caccgggacc tgaaccgata ataaccacat caaattgatt cattgtataa aaattttatg
    18001 caaaaataca tcttttaaac tggctgacat gtgctattgg acaaaaacaa attatctttc
    18061 ggtctgattt ttgattaaca tgacttatga agaaactaat tgtgcttttc gggttattta
    18121 taatggtatt cactttgtca gcattcaaag gtaaaacagc aacgcctttt aagaacctgc
    18181 aagtcttacc ggattctact acggaagaac agcttgatgc aatcatgcat gcctataacg
    18241 aatccttgaa tgtaaaatgc agccattgcc atataaaggg caatatggct tcagatgaaa
    18301 accctgagaa agaaatagcc cgcaagatga tgcgcatgac gaatgaaatc aatgaaaaat
    18361 acttcggtaa ggactccgga actgtaggct gtatgacttg tcataacggg aagttgcatc
    18421 catagttatt ttattcctct gacggtttat ccgactcttc tttttgttca ttgtcttgat
    18481 tgattgaaag ttgttcatta ttcacggcta catcctcatt atttaactga acgacttctt
    18541 cttttgtaaa tgtcatctgt tccattactg tattttcatc ccagacaatg ggttcaataa
    18601 taattgcatc actctcttca gaaacttctt tcaatcttct tctttcttct tttggtatat
    18661 agttttcttt atgacggcga cttaaaattt tctcttttcg agcggcataa tgttctttcc
    18721 gtttttcttt tcttattgca ctttttagct tttgttcctc acgtacaata gcaagttttg
    18781 cctttcgttc ttcttcacgt tgcaatcgga caattcgatc ttcttcggtt tcttctttcc
    18841 tgatttttag tttggaagga tgaatttcaa acacgtcatc aacatcaata tctactgtcc
    18901 accataagaa ttttagagtt gccttaccta tgctccgcat ataaatgggt tgtgccagta
    18961 aatttgttaa tgcctgaaac agagaagtaa tcgagctgat ttctgaattt gttgtaatga
    19021 tggtttccga acgtgctctt aatttaacag ctttcgtata attcatcttg cctattttta
    19081 ttccatccct gtttactaag tctgttgtta tttttaataa ggttatgggg agaaaaaagc
    19141 tgttgagcaa tttaagtacc gtttctgtct tcacactcaa taagcctatt tctttagtac
    19201 gtacttctac aatatcaacc gcccggtgtt gtgaggcttt tttgatgatg taagatttta
    19261 tcttctttgt cagccaacta aacatttgta tatttttata cttaaaggat tgttaaatcc
    19321 tcttaattaa agataggtat aaacgcaatt tcctaaattt acctaaagtt aagaactatg
    19381 aaagaaaatc ttacttattt aatagccata cccgtttttt tattttttgt cgttctcgag
    19441 tatttggcta tgcgcaaaaa agacccggaa tatacccgac tgaatgacgg catcacaaat
    19501 ttaaatattg gcgtggtgca tgtattgtca aaagtaattg tgggtgtttt cttgctttgg
    19561 gtgtatggat tagtatatga taatatccgc atttttacca tcccaaaagg aataatctcg
    19621 tttatatttt gcttgattgc ctacgatttc tttttttact gggcgcatcg cttcggacac
    19681 gaaatcaatt tgttttgggg agcgcacggt gtgcatcacc aaagtgaaga ttataatctc
    19741 accgttgctt tgcgtcagtc gtggtttcat gccgtaatgg cattttttat tttcttacct
    19801 atcccatttt taggcgttga ttccaaaaca tttttcagtg tactgaccat aaattcattc
    19861 tatcaatttt ggatacacac ccatcttatt aatcgtatgc cgcgctggtt cgaattcgtt
    19921 tttaattctc ctggtcatca cagagtacat catggcagaa acccaaaata tatagataaa
    19981 aaccacgccg gtatgtttat catttgggat aaactattcg gcacttttca agaagaagaa
    20041 gaaactcccg actatggtat tacgaagccc ttaaacagct ggaatccggc atgggcaaat
    20101 attgaatatt tttcaggcat gatacaaaat gcccgccgat ttaattttaa agataaactg
    20161 aaatacattt ttgcaaaacc cggttggcaa ccggattatg caggtggcac gatagctgta
    20221 aatgaggtta aaccggatta taaaaaatat aatgtgcgaa attccaaagg gctgaatatt
    20281 tatgtggttg ttcaatttct gattaccatc gttggcttag ttgcctatct gcaatattat
    20341 aagcaatggt cgttttttta tcaagccatc aattttattt taatcgtatt atctactgta
    20401 atttgcggag ccattttaga aaataaatcc tgggtaaaat atgctgagtt cataagaatt
    20461 gccatcattc ttatcgattt gaattcttta ttttatttca attatgtcga attttttgaa
    20521 tggtgtctgc tcatctccat aatcgcagga gtatatttta tcgtatggta tgcacttaat
    20581 atcaaccgta ttcatctttt cagggcgttg aaataattga agatttttct tttatttgta
    20641 taacatgaaa tttacctttt tgggaacggg tacctcgcaa ggcgttccgg tcattacttg
    20701 caactgcgaa gtatgtacat cttctgattt tcgggataac cgcacacgta cttcgctttt
    20761 aattcaatcc gctactacta ccgtagttat tgatacgggt ccggatttca gagcacaatt
    20821 actcagagaa aaagtgcagg atttagatgc cgtggttttc acccatgggc ataaagatca
    20881 tgtagccggg cttgatgata tccggccttt taactatatg cttcacaaaa caattaaggt
    20941 atatgcagaa cttggtgtgc aggatatttt gaaacgagaa tttgcctatg cctttcagcc
    21001 acaggaatat ccgggtgtgc cacaaataaa tttaattacg attgat
//
LOCUS       DAOQII010000009        20258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1214_length_20258_cov_5.447409, whole genome shotgun sequence.
ACCESSION   DAOQII010000009 DAOQII010000000
VERSION     DAOQII010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20258
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_20258_cov_5.447409"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="bamD"
                     /locus_tag="PLF48_00975"
     CDS             <1..435
                     /gene="bamD"
                     /locus_tag="PLF48_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNF47921.1"
                     /translation="CYAKTSPRYSLEQIDTEKAIAAFQVFVDKYPKSERVKEANTKMD
                     ELRAKLRKKAYESAYLYYKIGEYKAASVALRQFLQDYPEYENHEKIDFLISKSLFKYA
                     EGSYKDKKDERLADAQKEYAFFKTTYPASTHLSELEKLNKKN"
     gene            482..802
                     /locus_tag="PLF48_00980"
     CDS             482..802
                     /locus_tag="PLF48_00980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNF47922.1"
                     /translation="MSTAKKEEKQASAYIETRNLDELAAKTGNLYKSINVISKRSEQM
                     NAKLKEELHRKLEEFASATDNLEEVFENREQIEISRYYEKLPKTTITALNEFVNNETY
                     IKND"
     gene            939..2210
                     /gene="coaBC"
                     /locus_tag="PLF48_00985"
     CDS             939..2210
                     /gene="coaBC"
                     /locus_tag="PLF48_00985"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNF47923.1"
                     /translation="MTKSTPIKSKLNPLQDKKILLAVCGSIAAYKSAFLVRLLVKQGA
                     QVRVIMTKSATDFISPLTLATLSKNECLTAYTNSTQTNWNNHVDIALWADLMLVAPAS
                     ANTISKMANGICDNLLMGVYLSLRCPVFIAPAMDEDMWKHPSTHKNIEILKKNGNKLI
                     QVNSGELASGLIGEGRMAEPEEIVSILSMNMPESGKTDKKQPISLFAGTKILITAGPT
                     YEPLDPVRFIGNHSSGKMGIELAKKAVAMGADVTLVLGPSKVDVPDFITTIRVTSAQE
                     MYQACTAVFDQADVCIFAAAVADYTPKVVAGEKIKKSDDEFSLVLKKNVDIAYEFGKI
                     KKNNQISVGFALETQNEQEHAKAKIKKKNFDMIVLNSLNDKGAGFKYDTNKITIIQAN
                     NKLRTFELKNKEEVAADILAEIELLLRKKTK"
     gene            2207..3133
                     /locus_tag="PLF48_00990"
     CDS             2207..3133
                     /locus_tag="PLF48_00990"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNF47924.1"
                     /translation="MKKSIAFLLILGACLTIQAQELNCVIAVNSPKNQNIDPLVFKNM
                     ENAMMDFVNGRKWTNDVYKESEKIRCAITINIMETPSEGEYKATAIIQSQRPVFNSNY
                     HTLLLNVQDKTFDFAYADLQSLDYNDNGFTSQITSLLAYYSYLILAYDYESFGKDGGM
                     PYFQRALTVCNNVPQAEKTRYKNWSPFDATIGFTTGTINRYAYVDAWMNPRYKNLSDA
                     FYAYHLNGLDRMYQDAAVGRSMITMSLNMLQKIQQDNPNLTPLLSFFDAKSSELINIY
                     SKADMSEKAIAVQLLSSIDPTNAEKYKQINKK"
     gene            3208..4866
                     /gene="recN"
                     /locus_tag="PLF48_00995"
     CDS             3208..4866
                     /gene="recN"
                     /locus_tag="PLF48_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNF47925.1"
                     /translation="MLRTLSIKNYAIIESVLLEFDKGLNIITGETGAGKSILLGALNL
                     ILGKRADVQALRNTDEKCTVEASFYIKSYRLKSFFKSYDLDYEDETIIRREINSAGKS
                     RAFINDIPVTLDILKQLTEKLVDIHAQGETQNLLDKYFYCEILDELCKQTAVVQEYQV
                     HYAGFQQKQNRLKKLTETNIQLQKEFDFISFQLQELKEANIDEEEAAQIESELNLLQH
                     AETIKLQLNQALQFIDGNEQAALSLLLQANKHIAQLANINDVLLEMHDKLTGINEHLK
                     SMSRQITQIESATEYNAERVQYLSERQSTINNLLRKHHVVSATELLNIQHELQERVNQ
                     FNGSTDEIAILENELKQDKTALSATAQRVSANRAGKVKSFESAVSNLLKGLGMEYGYV
                     ELKNKSLINDEINEYGIDSYQLWFSPNKGIAPQPLETVGSGGEKSRLMLAIKSLVADT
                     ITLPTLIFDEIDTGISGDVAQKVGMVLKSLGNKHQVISITHLPQVAAKANNHLFVYKK
                     HDKEKTYTEIKVLNANERLHEIAVMLSGNNPPKEALENAKRLLN"
     gene            5001..6620
                     /locus_tag="PLF48_01000"
     CDS             5001..6620
                     /locus_tag="PLF48_01000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF47926.1"
                     /translation="MVNYNVRYFLEQCLYAVFRSKTNFEYEVFVVDNDSTDDSILMLK
                     NKFPQVILIANTENAGFSKANNQAMRLSNAEYILLLNPDTLIQETTLQQCIDKMDEDK
                     NTGALGVKMVDGSGRFLPESKRGFPSPVAAFSKMSGLSKLFPASKLFGQYHLTYLDKN
                     INHEVDVLSGAFMLLRRSVLDKIGLLDEDYFMYGEDIDLSYRIKQAGYKNIYFADTSI
                     IHFKGESTKKGSLNYIRVFYQAMIIFSQKHVSGSKGKALTIFLQTAIYLRAFMAIIQT
                     LFSGLFYPVVDAIMILGALLLSVRMFENIILSAKHVRYPASFYQINIPLYIGFWLLTL
                     WFWGVYQKGNKWKHLLAGLLSGTVLISLVYSFFPLALRSSRGIILSSFLLNIVFLSSY
                     RFLLTWINGKWSNYVKDEKRYIIIGEGKEAKAISGNLILYNKNRKYIGFVSLKNDREQ
                     TDYLGNMESLAEIIEVYSPDELLFSTDTVPMQTIVQAMSKIKGKLDYKLISKNTSIIG
                     SSSKNEGGDVYTFDVDLQNGRSFLQRIRAWL"
     gene            complement(6628..8658)
                     /locus_tag="PLF48_01005"
     CDS             complement(6628..8658)
                     /locus_tag="PLF48_01005"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNF47927.1"
                     /translation="MADSQYPILLSELDLGFTKLKNRVLMGSMHTNLEEAKDGFQKLA
                     KFYAERARGGVGLIVTGGMAPNWEGWLKPFAERMTNSSHVKKHKMVTEAVHEEGGKIA
                     MQILHAGRYAYHPLSVAPSRIQAPINPFKPRALSTWGVKRTINDFADCAALARDAGYD
                     GVEIMGSEGYLINQFLVEHTNKRTDEYGGSYENRMRFPLEILAKARQKVGKDFIIIYR
                     LSMLDLIEDGSNWDEIVQLAKAIEKAGATIINTGIGWHEARIPTIATSVPRAGFTWVT
                     KKLKNEVSIPLCTSNRINMPSVAEKVLEDGCADMVSMARPMLADENWVAKAKAGKEKE
                     INTCIACNQACLDHTFSNKLSSCLVNPRACHETELNYLSAVDKKKIAVVGAGPAGLAF
                     SSIAAERGHQVTLFEADAEIGGQFNMAKKIPGKEEFYETLRYFEVQLRKHGVNVKVNT
                     RVSGDDLKNFDEIVLATGILPRQVTIEGVNHKKVLGYIDVLKNKVAVGKKVAVIGAGG
                     IGFDVSEYLTQKGESPTLNTKEWMQEWGVDMSNEARGGVEGIQQHIEAPAREVVMFQR
                     TAGKVGAKLGKTTGWIHRTTLKMKKVKMIDGVEYVKIDDEGLHYIDKSKQPQVYTCDN
                     VVLCAGQVSLRELEQPIADLGIPMHVIGGAYEAGELDAKRAINQAARLAADI"
     gene            complement(8777..9229)
                     /gene="dtd"
                     /locus_tag="PLF48_01010"
     CDS             complement(8777..9229)
                     /gene="dtd"
                     /locus_tag="PLF48_01010"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNF47928.1"
                     /translation="MRAVIQRVAKASVTIDNKVNGSINSGLLVLLAIEENDTHTDIEW
                     LSKKITQLRIFSDVDGKMNLSVKEIGGDILLISQFTLFASTKKGNRPGFTRSAKPEIA
                     IPIYEQFILSLQIQLQKKIQTGIFGADMKVELLNDGPVTIIIDSKNPE"
     gene            9298..10521
                     /locus_tag="PLF48_01015"
     CDS             9298..10521
                     /locus_tag="PLF48_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF47929.1"
                     /translation="MAQESPIKKTWKRLLRNKPAIISLVVIVLAIILAFLGPAVAMDK
                     TPDADDQILELANVNPGFSTKMLLVRKNREENKVNPATAFFMGRENPYQQIPISSYQF
                     NDSGQIVVQAFKGEEHKAEEMRFSLADVVYPLSATSPDIHLENGKLNFTDFNGQPVSA
                     DIQKLQKETTDKHIVLKKYRLGADDFGRDILSRLILGIRVSLSVGIVAVIISMTIGIF
                     MGAIAGYFGGRTDDVITFLINIIWSIPTILLAMAISFSLGAILDSYITIFIAIGLSMW
                     VETARIVRGQVLSVREMEYIQAAKGLGFSHFRIILKHILPNIIGPIMVLAASDFAAAI
                     LIESGLSFIGLGVKPPTPSWGNILNAHFHYLTSNKAFLAIAPGICIMVMVLVFNLLGN
                     GLRDAFDVKGKIDTQ"
     gene            10784..14122
                     /locus_tag="PLF48_01020"
     CDS             10784..14122
                     /locus_tag="PLF48_01020"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNF47930.1"
                     /translation="MNKQYKLVNAVVAFVVFAISLWQYTSTMETAGSLWDCGEFTSCA
                     YKLQVAHPPGAPLFLMMGRLFTLLNAKNPVLMINFLSALMSALCVMFLFLTTTLIVKK
                     LLVNDKDEVISLDKTLAIVGSGAVAALACSFSDTMWFSAVEGEVYASSMFFISIVLWA
                     IMKWDDVAEEPFADRWFIFAAFMVGLSLGVHLMSLLVFPTASLVYYYRRFKPSLFGLA
                     VMFVLGFVILGMVQVGVIQFMTLKAQQMEFFFVNSIGLPFNTGAFIYFIILISALVFA
                     IYYGTRKDFNSMIYIGLFHFILFFSVLSVGMGSFLILMLLWAIGYAILRYGFNYENDK
                     ALFWGVFAGLPVLVYNLFKKYSPRLDGRDPITVKTMQTVLISLLVIIIGFSSYMMVPI
                     RAASNPPINMNSPKDVFSLLSYLNREQYGDRPLVKGPLYTARPVDYDIKGKEYYQDLK
                     TGKYEVKGEKIGYKYDPADEILFPRMGPTNDGESADGLYRYWTGFQGATPSMGDNLTY
                     FFKYQIGYMYKRYFLWNFAGRQDDYQGMVGTVGMDGDWLSGIPFIDNAHLGSQEGLPY
                     QILNQKARNYFYMLPLLLGIIGLVFMLYRNQHYTIILALLFFATGIALIIYTNQPPRE
                     PRERDYVLVGSFFTFCIWIGTSVAAIYDFLKSKKLPGLPVAVLSTAMCLFVPYLMGKT
                     GWNDHNRNGRYMARDFAANYLKSCPPNAILFTQGDNDTYPLWYAQEIEGIRTDVRIVN
                     LSLLGVDWYIDCLMRASNKSGPVPFYKAFTPDKYRGSKRDMIQYNETNSFAGQTLDLT
                     NVMDFILSDDPQFMAQAGRNDQVNYLPTTKFKMKVNKEAIISNNVVPEQFKDKVVDEM
                     VWDLGKNRIIKFDLALLAMVAAQDWSRPICFSNTVEEGYYQGLGKYLVQEGEVLRLVP
                     CLFLENQKGYYTMNTQRSYDIVMNDFKYGGLGDREMFVDENSARIMNTLKSVHFGIAD
                     DLVRNGRQADAVKVLDRAKKEFQYVNAPYYAPNNRFFNILTVQWIDLYYRAGAPANAK
                     SIKELYIKDLKDCLRFYNLPNDYAALYANEKKSAEDIVKRMEMLAIQYKDNEFMAELN
                     KAFPTLVQKSTIDLTQQVPNQIFR"
     gene            14175..14591
                     /gene="mscL"
                     /locus_tag="PLF48_01025"
     CDS             14175..14591
                     /gene="mscL"
                     /locus_tag="PLF48_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNF47931.1"
                     /translation="MGMLQEFKEFAMKGNLIDMAVGVVMGGAFGKVVSAFIDGMVMPA
                     IGMISGGVDFNDKVFVLKKAVAASADGSVAATPEVAIKYGAFISQVITFLVVAFVVFM
                     IIKAINKMKKEEAAATPPAPPAEEVLLTEIRDLLKK"
     gene            complement(14739..15458)
                     /locus_tag="PLF48_01030"
     CDS             complement(14739..15458)
                     /locus_tag="PLF48_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNF47932.1"
                     /translation="MSLEDFKKKRPYRQRNWTQVNASNSWSMFRIMSEFVDAYEKMDK
                     IGPCISIFGSARTKPENPYYSMATKVAERLVEEGYGVITGGGPGIMEAGNLGAQNKDG
                     KSVGLNIELPFEQGHNPHIDHDKVITHHYFFVRKVVFVKYAQGFVYLPGGFGTFDELF
                     EVMTLVQTKKIDRVPIVLMGKAFWSGLIDWIKSEVLGNQHNINEEDLNLFLITDDVEE
                     AVQHINNFYDTEHHELAPNFS"
     gene            complement(15524..17068)
                     /locus_tag="PLF48_01035"
     CDS             complement(15524..17068)
                     /locus_tag="PLF48_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNF47933.1"
                     /translation="MNTSLIFVCLIAYFIFLFVIAWYTSRNASNKDYFVGGRSSRWYI
                     VAYGMVGTSLSGVTFISVPGKVGTAHFAYFQVVLGYLIGYFVVAYILLPLYYRLNLTS
                     IYTYLQHRFGDISYKTGAAFFILSRTLGATLRLYLVINVLQIFLLPEGMGYFTLAAFV
                     ILTMILLYTFKGGVKSIVWTDMLQTTFMLLALVTCVWYIKNQLGMSVGGMMSALQEKG
                     YTQIFMTDWLKPNFFIKQIIGGMFITITMTGLDQEMMQKNISCKNLGDAQKNMVTFSF
                     ILVAVNLIFLVLGGVLFLYGEQLNIAHIADGKFQLWDAVSHIFVAKNSDDLFPTIALN
                     YLTPFISILFIIGLISALFPSADGALTALTASFCIDILGINQNENLSEEDRTKTRLKV
                     HFTFAFVFLLFVLGFKILNKEAIIDTLLKIASYTYGPLLGLFMFGILTKRTIKDKLTP
                     YICIASPVICFLLDMNSTKLIPDFKFGPEMLLWNGLLTFLGLYLISGSSKQGDNILDD
                     SLISSK"
     gene            17205..17822
                     /gene="recR"
                     /locus_tag="PLF48_01040"
     CDS             17205..17822
                     /gene="recR"
                     /locus_tag="PLF48_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNF47934.1"
                     /translation="MQFPSRLIEDAVNAFAKLPGIGKKSALRMVVYLLQKETADTEQI
                     AQAILRMRKEIQFCEVCHNVSDAAICSVCGNPHRDKSTICIVENFRDIIAIENTKNYN
                     GVYHVLEGLISPIDGIGPDKLNIAKLMERVQSGEVKEIIMALNPTLDGDTTIFYLSKQ
                     LAAMPIKITTIARGISFGGELEYVDEITLSRSIQARLPYERYMSR"
     gene            complement(17964..19055)
                     /locus_tag="PLF48_01045"
     CDS             complement(17964..19055)
                     /locus_tag="PLF48_01045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF47935.1"
                     /translation="MSVWLIVLGSCIVFQTIYLIVLYFTLIGKTKTPDKAPSSIFPKV
                     SIVICSKNEEFNLKQHLPKIIEQDYPDVEILIVDDGSTDNTQAVLQTFGNKIKTVFIP
                     SEEKRGLGKKYALLQGIKSANGEIILFTDADCYPASKNWVKEMVAHFNDKTDIVLGIG
                     LYEKRKGLLNKLIAYETAQTALQYLGFAALGNAYMSVGRNVAYSKDLLQQLDWSDKDY
                     AIVSGDDDLAIQQLANKNNVDVCLNPEAFTFSHPKTTWTSWLKQKSRHTQSSWQYKPS
                     HKLLLFLFGFTKMTIWAITLCLLPVDFQNVSSFILLYSLVLLGVYHQLNNHLSLFTRW
                     YFAPITDFILSFSYLFIGIKNTFSSSKTW"
     gene            19104..20234
                     /gene="tgt"
                     /locus_tag="PLF48_01050"
     CDS             19104..20234
                     /gene="tgt"
                     /locus_tag="PLF48_01050"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNF47936.1"
                     /translation="MTFSILHTDNQSSARTGMITTEHGNIETPIFMPVGTQATVKAVQ
                     FSELENEIKAQIILGNTYHLYLRPGLDILRAAGGLHAFNTWQKPILTDSGGYQVYSLS
                     HRRKLTEEGVVFQSHIDGSKHLFTPENVMDTQRIIGADFIMAFDECTPYPCEYAYARK
                     SLKITHDWLLRCIRRFDATEPLYGYEQTLLPIVQGSVYKDLRAESAEFIANQNRSANA
                     IGGLAVGEPAEIMYEMTALVNSILPKDKPRYLMGVGTPANLLESIALGVDMFDCVMPT
                     RNARHGLLFTSEGIINIKNQKWKNDFSPIDATIPASTSRKHTKAYLRHLFVVEEYLGA
                     QLASIQNLSFYLWLMQEAKNRIKDGTFSTWKDVAIRKFMTRL"
BASE COUNT         6247 a         3877 c         3992 g         6142 t
ORIGIN      
        1 tgttatgcca aaacatcccc tcgctattca ttagagcaaa ttgatacaga aaaagcgatt
       61 gccgcatttc aagtttttgt cgataaatac cccaaaagcg aacgtgtgaa agaagccaat
      121 accaaaatgg atgagctgcg agccaaactt cgtaaaaaag catacgaatc cgcttatctt
      181 tattacaaga taggggaata taaagctgca tctgtggcac tccgtcagtt tttgcaagat
      241 tatcccgagt atgagaatca cgaaaaaatt gatttcctta tatccaaatc tctctttaaa
      301 tacgctgaag gaagctataa agataaaaaa gacgaacgct tagccgatgc acaaaaagaa
      361 tatgctttct tcaaaacaac ttatcccgca agcacacatt tgagtgagct ggagaaattg
      421 aacaaaaaga actaaaattt ataatcagga tttaaaattc ataatctata atttactaac
      481 catgtcaact gcaaaaaaag aagaaaaaca agcttcagcc tatatcgaaa ccagaaattt
      541 agatgaatta gcagctaaaa ctggcaactt gtacaagtct attaatgtta tttcaaaacg
      601 ttctgagcag atgaatgcaa aattaaaaga agaattgcac cgcaagctgg aagaatttgc
      661 ttctgcaaca gataatctgg aagaagtttt cgaaaacaga gagcaaattg aaatctctcg
      721 ctactacgaa aaattgccta aaacgacaat tacggcactg aatgagttcg taaataatga
      781 aacttatatc aaaaacgact aattaccgat ttgtctttaa gtgaaggcta agcggtattt
      841 gtgggcgcgt gtgctttatt ggtttttatt taatttcctg taaaaaggct ttttctgctt
      901 attatttcag tatcagattt tgtaaaaaac actaaaattt gacaaaatca actcccataa
      961 aatccaagct taatccgttg caagataaaa aaatcttgct ggcagtttgc ggaagcatag
     1021 cggcttataa atccgcattt ttagttcgtt tgctagtaaa acaaggagca caagtcaggg
     1081 taattatgac aaaatcagct accgacttta tttctccgct aacgctggca acgctctcca
     1141 aaaacgaatg cctgacagct tatacaaatt ccacacaaac caactggaac aaccatgtag
     1201 atattgcact ttgggctgat ttgatgcttg ttgcccctgc ttcagccaat accatttcaa
     1261 aaatggccaa tggtatatgt gataatttat tgatgggcgt ttatctttct ttgcgatgtc
     1321 cggtttttat cgccccggct atggatgagg atatgtggaa acatccatct acccataaaa
     1381 atatagaaat acttaaaaaa aacggaaata aactgataca agttaactcc ggcgaactag
     1441 ccagtggact tatcggcgaa ggcaggatgg cagagccgga agaaattgtt tctattttgt
     1501 cgatgaatat gcccgaatcc ggaaaaactg acaaaaaaca acccatttct ttgtttgccg
     1561 gtacaaaaat actgattaca gcggggccta cttacgaacc actcgatcct gtccggttta
     1621 tcggcaatca ttcatccgga aaaatgggca ttgaactggc aaaaaaagca gtagccatgg
     1681 gtgctgacgt aacgctcgtt ttaggtccgt ccaaagtgga tgttcctgat tttattacta
     1741 ccattcgggt tacctctgcg caagaaatgt atcaggcttg tactgccgtt tttgaccaag
     1801 ctgatgtctg catatttgcc gccgcagttg cagattatac accgaaagta gttgccggtg
     1861 agaaaattaa gaaatcggat gatgaattta gtttggtctt gaaaaaaaat gtagatattg
     1921 cttatgagtt cggaaagatt aagaaaaaca atcaaatttc tgttggtttc gcactcgaaa
     1981 cccaaaatga gcaagaacac gctaaagcca aaataaaaaa gaagaatttt gatatgattg
     2041 tcctgaattc actcaacgac aaaggtgccg gattcaaata tgacaccaat aaaattacga
     2101 ttatccaggc aaacaataaa ttgcgtacat ttgagttaaa gaataaagaa gaagtagctg
     2161 cagatatact ggcagaaata gagttgttgc tgcgtaaaaa aacaaaatga aaaaaagtat
     2221 tgccttttta ttgattttgg gtgcttgttt aacgatacag gctcaggagt tgaattgtgt
     2281 aattgccgtt aattctccca aaaaccaaaa tatagacccg ctcgttttca aaaatatgga
     2341 aaacgccatg atggattttg taaatggtcg aaaatggaca aatgatgttt ataaagaatc
     2401 tgaaaagata agatgcgcta ttacgattaa tatcatggaa acgccttccg aaggagagta
     2461 caaggctaca gcaattattc aatcacaaag accagtattt aattccaact atcacacctt
     2521 attattaaat gtacaggata aaacctttga ttttgcttat gccgacttgc aaagtctgga
     2581 ttataatgac aacgggttta cctcacagat tacttcgctt ttagcttatt attcctattt
     2641 gatattggct tacgattacg aatcttttgg gaaagacgga ggcatgcctt acttccagcg
     2701 ggcattaacg gtttgcaata atgtgcctca ggctgaaaaa acaagatata aaaactggag
     2761 tccctttgat gctactatcg ggtttaccac cggtaccatt aatcgttacg cctatgtaga
     2821 tgcctggatg aatcctagat ataaaaatct gagtgatgca ttttatgcct atcatctgaa
     2881 tggattggat cgcatgtatc aggatgctgc cgtgggcaga agtatgatta ccatgtcact
     2941 caatatgctg caaaagatac aacaggataa tccgaatctc acccccttgc tttcattttt
     3001 tgatgccaaa tcatcggaac tgataaatat ttattcaaaa gcagacatga gcgaaaaagc
     3061 cattgcggtg cagttattat cttcgataga ccctacgaat gcggagaaat ataagcaaat
     3121 aaataaaaag taagcatcaa tttattcttt gttatttttt gaaattttat ccaactctgg
     3181 ttcgataatt ccttattttt accaagtatg ttgcgcacgc tttctataaa aaattatgct
     3241 ataatagagt ctgttttgct cgaatttgat aaaggattga atatcattac cggcgaaaca
     3301 ggtgccggaa aatccatatt gctgggtgca ttgaatttga ttttaggcaa acgtgctgat
     3361 gtgcaagcat tgcgcaatac ggatgaaaaa tgtaccgtag aagcatcttt ttatataaaa
     3421 tcttatcggc tcaaatcttt cttcaagtcg tatgatttgg attatgaaga tgaaacgata
     3481 atacggcggg aaattaattc tgccgggaaa tctcgtgcct ttatcaatga tattccggtc
     3541 acactggata ttctaaaaca gctgacagaa aaattagtgg atatacatgc ccagggtgaa
     3601 acacaaaact tgctggataa atatttttat tgtgagatac tggatgagct ctgtaaacaa
     3661 acagcagttg tacaagaata tcaagtacat tatgccggat tccagcaaaa acaaaaccgc
     3721 cttaaaaaac tgacagaaac caatatccag ttgcaaaaag aattcgattt tatttcattt
     3781 cagttacaag aattaaaaga agcgaatatt gatgaagaag aagccgcaca aattgagagt
     3841 gaattaaacc tactccagca tgccgaaacc ataaaactac agcttaatca agctttacag
     3901 tttattgacg gcaacgagca ggcggcacta tccctcttat tacaagctaa taagcatatt
     3961 gcccaactgg ccaacatcaa tgatgtattg ctcgaaatgc acgataagct aacgggcatc
     4021 aacgagcacc tgaaaagtat gtcgaggcag ataacacaaa tagaatcggc taccgaatac
     4081 aatgctgaac gggtacagta tttatcagaa agacaaagta ctattaataa tttactgcga
     4141 aagcatcatg ttgtttcagc aactgaatta ttgaatatcc agcacgaatt acaagaacgc
     4201 gttaatcagt ttaatggctc gacagatgaa atagctattt tggaaaatga acttaagcaa
     4261 gataaaacag cactatcagc cacggcgcaa cgggtgtcgg caaaccgggc aggcaaggta
     4321 aaatcatttg aaagtgccgt cagtaatttg ctgaaaggtt tgggtatgga atatggttat
     4381 gttgaattaa aaaataaatc gttaattaac gatgaaatca atgaatacgg aattgacagc
     4441 taccaacttt ggttttctcc aaataagggc atagccccgc agccactgga aacggtaggc
     4501 agcggaggcg aaaaatcgag attaatgctg gctattaaaa gtttggtggc agataccatt
     4561 accttgccta cgttgatatt tgatgaaatt gataccggca tttcgggaga tgttgcacaa
     4621 aaagtaggga tggtattaaa atcgcttggc aataagcatc aggttatttc gattacgcat
     4681 ttgccacaag tagccgccaa ggccaataat catttgtttg tttataaaaa acacgataaa
     4741 gaaaaaacct ataccgaaat aaaagtgctg aatgctaatg agcgcctgca tgaaattgct
     4801 gtgatgctga gtggcaacaa ccctccaaaa gaagccctgg aaaatgcaaa gcgattgtta
     4861 aattaatcac gatacatcag cattgagtta tcaattttat ctgtaaaacc gtacctttat
     4921 ttccctccga tttatattca ctatttagaa ataaagagta aaaaggagta aatagtctta
     4981 ttctgaagct gagtatcgtc atagtgaatt ataatgtgcg ctatttttta gagcaatgtc
     5041 tgtatgccgt atttcgcagc aaaaccaatt tcgaatacga agtttttgta gtggataatg
     5101 attcaacaga tgattcaata ctcatgttga aaaacaaatt tccgcaagtt atcctgattg
     5161 ccaacacgga aaatgccgga ttttcaaaag ccaataatca agctatgcgc ttaagcaacg
     5221 ccgagtatat tttattgctc aatccggata cgctgataca ggaaactacc ttgcagcaat
     5281 gtattgacaa gatggatgaa gataaaaata caggtgcatt aggggttaaa atggttgacg
     5341 gcagtggtcg gtttttaccc gaatccaaac ggggatttcc atctccagtg gcggcattca
     5401 gcaaaatgag tggcttgtca aaattatttc cagcatcaaa attatttgga caatatcatc
     5461 tgacttattt agataaaaac ataaaccatg aggtagacgt actgagtggt gctttcatgt
     5521 tgctgcgccg ttctgtctta gataaaatcg gcttattgga tgaagattat tttatgtacg
     5581 gtgaggatat agatttgtcg taccgcataa aacaagcagg ctataagaac atttattttg
     5641 cagatacttc tatcatccat tttaagggtg aaagcaccaa gaagggaagt ttgaattata
     5701 tacgcgtgtt ttatcaagcc atgataattt tttcgcagaa acacgtatct ggttcaaagg
     5761 gtaaggcact caccattttc ttacaaacag ccatttattt gcgagcattt atggcaataa
     5821 ttcaaacgct gttctcgggt ttgttttatc ctgtagtaga tgcaataatg atattgggag
     5881 ctttattact ttcagtccgc atgtttgaaa atattattct atcggctaag cacgtgcgct
     5941 atcctgcatc tttttatcaa ataaatattc cgctctatat tggcttttgg ttgcttactc
     6001 tttggttttg gggtgtctat caaaaaggaa acaaatggaa acatttactc gccggactgc
     6061 taagcggtac tgtattgatt tcgttagttt attctttttt ccctttggca ttgcgttcat
     6121 caagagggat tattttaagt tcatttttgc tgaatatcgt tttcttgtca tcttatcgtt
     6181 ttctgttaac ttggataaat ggaaaatgga gcaattatgt aaaagatgaa aagcggtata
     6241 ttattatcgg cgaaggcaag gaagctaagg cgataagtgg gaacttaatt ttatacaata
     6301 aaaacagaaa atacataggg tttgtttcct taaagaacga cagagaacaa acagattatt
     6361 taggaaatat ggaaagcctt gctgaaatta ttgaagtgta ttcgccggac gaattattat
     6421 tttctactga cactgtaccc atgcaaacca tcgtacaggc aatgtcaaaa ataaaaggaa
     6481 aattagacta caagcttatc tctaaaaata cttctatcat aggcagttct tcgaaaaatg
     6541 aaggtggcga tgtctacacg tttgatgtcg atcttcagaa tggaagatct tttttgcaac
     6601 gtatcagagc ctggctataa aatcaaatta aatatcagct gccagccttg ccgcctgatt
     6661 gatagctcgt ttggcatcca gctccccggc ttcataagct ccgccaataa catgcatggg
     6721 aatccccaaa tctgcaatgg gttgctccag ttctctcaac gaaacctgtc cggcacacaa
     6781 taccacgtta tcacaagtat aaacttgagg ctgtttgctc ttgtctatat agtgtaaccc
     6841 ttcatcgtca atctttacat attctacacc gtctatcatt ttcacttttt tcattttcag
     6901 cgtagtgcga tgtatccagc ccgttgtttt gcctaatttg gcgcccactt ttccggcggt
     6961 acgctgaaac atcacgacct ctcgtgccgg agcttcaata tgctgttgaa tgccctcaac
     7021 tcccccacgg gcttcattgc tcatatccac accccattcc tgcatccact cttttgtatt
     7081 cagtgtcgga ctttcgcctt tctgcgttaa atattcagat acgtcaaaac caattccgcc
     7141 tgcaccgata acggcaactt ttttgcctac tgccacttta ttttttaaca catcgatata
     7201 acccagcact ttcttgtggt tgacaccttc aatcgtgact tgtctgggta gaatacccgt
     7261 agccagcacg atttcgtcaa aatttttcaa atcatcacca ctcacacggg tattgacttt
     7321 tacattcact ccgtgttttc ttaattgaac ctcaaaatag cgcagtgttt cataaaactc
     7381 ttctttcccc ggaatttttt tagccatatt aaattgtccg ccaatttcag catccgcttc
     7441 aaacaaggtt acttgatgcc ctctttctgc cgcaatactt gaaaatgcaa gtccggcagg
     7501 gcctgcacct actacagcta tttttttctt gtcaactgcc gacaggtagt tcagttccgt
     7561 ttcatggcag gctctcggat tgaccagaca agaagataat ttatttgaaa aagtatggtc
     7621 taaacaggcc tgattacagg caatgcacgt attaatttct ttttctttcc cggctttagc
     7681 ctttgccacc cagttttcgt cagccagcat cgggcgtgcc atcgatacca tgtctgcgca
     7741 gccatcttcc agcactttct ctgctacact tggcatattt attctgttgg atgtacaaag
     7801 cggaatggaa acttcatttt tcagtttctt ggttacccat gtaaatcctg cccggggcac
     7861 agaagtcgct atagtcggta ttcgcgcttc atgccaacct atccctgtgt ttataatagt
     7921 tgcccctgct ttttctattg ccttggctaa ctgtacgatt tcatcccaat tactgccatc
     7981 ttcaatcaaa tctaacatcg acaaacgata aatgataatg aaatcctttc ccactttttg
     8041 acgtgcttta gcaaggattt ccagtgggaa acgcatgcgg ttttcatagc tgcccccgta
     8101 ttcatctgta cgtttgttgg tatgttctac cagaaactga ttgattaaat agccttcgct
     8161 acccattatt tctaccccat catatccggc atcgcgggct aaagctgcac aatcggcaaa
     8221 atcgttgatc gtgcgtttta ctccccatgt actcaaagca cgaggcttaa acggattaat
     8281 aggagcttgt attctgctgg gtgctacact tagtggatga taggcatatc gcccggcatg
     8341 aagaatctgc atggcaattt tgccgccttc ttcatgaacg gcttcagtaa ccatcttatg
     8401 tttttttaca tgcgatgagt tggtcatgcg ttctgcaaaa ggtttgagcc atccttccca
     8461 attaggagcc atgcctccgg taacaatcaa tcctacacca cctctggctc tttcggcata
     8521 aaatttagcc agcttttgaa agccgtcctt cgcttcttcg agattagtgt gcatggaacc
     8581 catgagcaca cggtttttaa gcttagtaaa acccaaatca agttcggata atagaatagg
     8641 atattgtgaa tcagccataa taaatctgtt ggtgcggaaa gataagcaaa agccataaaa
     8701 gtttttataa aatggtcatg ttgattcaaa aacccaacga tgcagaatgg atttattgaa
     8761 aataaaaatt acttttttat tccggattct tggagtcgat gataattgtt acaggaccat
     8821 cattcagcaa ttctactttc atgtctgcac caaatatgcc ggtttgtatt ttcttttgca
     8881 gctgaatttg caatgacaag ataaattgct cataaatggg gatggctatt tccggttttg
     8941 cagaacgtgt aaagccgggt ctgttgcctt ttttcgtgga agcaaacaac gtaaattgag
     9001 aaatcaataa aatatcgccg cctatctctt taaccgataa attcatttta ccatccacat
     9061 cagaaaaaat gcgaagttgg gttattttct tactaagcca ttctatgtcg gtatgtgtat
     9121 cgttttcttc tattgctaat aaaaccaaaa gtcctgagtt tattgaccca tttactttat
     9181 tgtctatagt aactgatgct ttagcaacac gttgaataac tgcccgcata tttcaaagtt
     9241 aagcgtttta tatagcattc ctaattctat ttcttttatt atctttagcc attgcctatg
     9301 gctcaagaat ctcccataaa aaaaacatgg aagcgcttat tgcgaaacaa acctgcaatt
     9361 atcagtcttg tagtgattgt gctggctatt atattggcct ttctcggtcc ggcagtagct
     9421 atggataaaa caccggatgc ggatgatcag atacttgagt tggcgaatgt caatcccggg
     9481 tttagtacta aaatgctgtt agtccgcaag aacagggaag aaaacaaagt caatcccgct
     9541 actgcatttt ttatgggcag agaaaaccct tatcagcaaa ttccgatatc gagctatcag
     9601 tttaatgata gcggacaaat agtagtgcaa gccttcaaag gcgaagagca taaagcagaa
     9661 gaaatgcgtt tttcgctggc agatgtcgtg tatcctttat ctgctacatc gccggacatc
     9721 catcttgaga acgggaaact gaattttaca gactttaatg gtcaacctgt ttctgccgat
     9781 atccaaaagt tgcaaaaaga aactactgat aaacatattg tgttaaaaaa ataccggctt
     9841 ggtgcagatg atttcggcag agatatttta agcaggctga tactgggtat tcgtgtatct
     9901 ttatcggtgg ggattgtggc ggtaattatt tctatgacaa tcggcatttt tatgggtgct
     9961 attgcggggt attttggtgg aagaacggat gatgttatca catttcttat taatattatc
    10021 tggtcaattc caacgatatt gttagctatg gcaatcagtt tttcattagg ggctatttta
    10081 gacagctata ttactatctt catagcaatt ggtttgtcga tgtgggtaga aaccgcacga
    10141 atagtaagag ggcaggtgct ttctgtcaga gaaatggaat atatacaagc cgctaagggc
    10201 ttgggatttt ctcatttcag aattatacta aagcatattc tgcctaatat tatcggtccg
    10261 attatggtgc ttgccgcatc cgattttgcc gctgctatcc tgattgaatc agggctgagt
    10321 tttataggtc ttggcgtaaa gccacctact ccatcatggg gtaatattct gaatgcgcat
    10381 ttccattacc tcacttcaaa taaggcattt ttagccatag caccgggtat ttgcattatg
    10441 gtaatggtgc tggtgtttaa cttattgggc aatggactgc gagatgcctt tgacgtaaag
    10501 ggcaagattg atactcaata acaggttcag tgataagcgt ttccatttaa aagcagtcga
    10561 ttttttataa gaaacccttc ctttcaattt ctcatctttg ataaaggtag aggaaaaaat
    10621 gagaaatcaa ctattttttt caccttaatt ttcttaaccc taactcaggt taataaatct
    10681 gtacttacaa tagagtgtct ttatatgtcg tttttgcatt aattcacatc tgtctcaagg
    10741 tgttaatagt tttatttttc ttatttttgg caaaattctt tgcatgaata aacaatacaa
    10801 actggtaaat gccgtagtag catttgtcgt ttttgcaatt tctctctggc agtatacttc
    10861 aacaatggaa accgcgggca gtttatggga ctgtggcgag ttcacctctt gtgcttacaa
    10921 attacaggta gcgcaccccc cgggagcccc tttatttttg atgatgggca gattgtttac
    10981 tttgctcaat gcgaaaaatc cggtattaat gatcaatttc ttgtcggcgc tgatgtcggc
    11041 attatgcgta atgtttttgt ttctcaccac cacattaatt gtaaagaaat tattggtaaa
    11101 tgacaaggat gaagttatca gtctggataa aacattagcc atcgttggtt ctggtgctgt
    11161 ggcggcatta gcatgttcat tttccgatac tatgtggttt tctgcagtgg aaggagaggt
    11221 atatgcctct tccatgttct ttatttccat agtgctttgg gctattatga aatgggatga
    11281 cgtagcagaa gaaccatttg ccgatcgatg gtttatattc gctgcgttca tggtaggctt
    11341 gtcattaggt gtccacttaa tgtcattgtt ggtattccct actgcaagtt tggtctatta
    11401 ttatcgtcgt ttcaaaccaa gtttatttgg cttagccgtg atgtttgtcc tgggttttgt
    11461 tattttgggt atggtgcagg tgggtgtcat tcaatttatg acacttaagg cacaacaaat
    11521 ggaattcttt tttgtaaaca gtataggact gccatttaat acgggagcat ttatttattt
    11581 tatcatcctg atttctgctt tagtttttgc catttattat ggcaccagaa aagactttaa
    11641 ttccatgatt tatatcgggt tattccattt tattctattc ttctccgtat tgtctgtggg
    11701 gatgggaagt tttctgatat taatgctgct ttgggcaata gggtatgcta ttttgcgcta
    11761 tggttttaat tatgagaatg acaaagcctt gttttggggc gtgtttgcag gtttgcctgt
    11821 attggtctac aatctattta aaaaatattc tcctcgttta gatggcagag atcctattac
    11881 cgttaagacc atgcaaaccg ttttgatttc cttattagtt atcattattg gtttttcgag
    11941 ttatatgatg gtacctatac gggcagcttc taatccgcct ataaacatga actctcctaa
    12001 agatgttttt agtcttttgt cttatttgaa ccgcgaacaa tatggcgaca gacctttagt
    12061 aaaaggacct ttgtatacgg cacgccctgt cgattatgat attaaaggaa aagaatacta
    12121 tcaggatttg aaaaccggga aatatgaagt gaaaggtgaa aaaataggct ataaatacga
    12181 cccggcagat gagatattat tcccacgcat gggtcctact aatgacggtg agtctgcgga
    12241 tggtttatac cggtattgga caggctttca gggtgccacg ccttccatgg gcgataatct
    12301 gacttatttc ttcaaatacc agattgggta tatgtataag cgttatttcc tgtggaattt
    12361 tgcaggaaga caagacgatt atcaaggtat ggtaggcaca gtaggtatgg atggtgactg
    12421 gctttccggt attccgttta tcgacaatgc ccacttaggc tcacaagagg gcttgcccta
    12481 tcagattctc aatcaaaaag cccgcaacta tttttatatg ctgccacttc tgttaggtat
    12541 catcggctta gtgtttatgc tataccgtaa tcagcattat accattattc tggcattatt
    12601 gttttttgca acaggcattg cgttaattat atataccaat cagccaccgc gagaaccacg
    12661 tgaaagagat tatgtgttag tgggttcatt ctttaccttt tgtatatgga taggtacttc
    12721 ggtcgccgcc atttacgatt ttcttaagag caaaaaacta cccggtcttc cggtagctgt
    12781 tttatcaaca gcaatgtgct tatttgttcc ttatttaatg ggcaagacgg ggtggaatga
    12841 ccataaccgt aacggacgct atatggctcg tgattttgca gcaaattatt tgaaaagctg
    12901 tccgccaaat gccatattgt ttacacaagg cgataatgac acctatccgc tttggtacgc
    12961 acaggaaata gaaggaattc gtactgatgt gcgaattgta aatctgagtt tgttgggtgt
    13021 agactggtat attgactgtc tgatgcgtgc ttctaataaa tcaggtcctg tgccatttta
    13081 taaagccttt actccggata aataccgtgg aagtaaacgc gatatgatac agtacaacga
    13141 aacgaatagt tttgccggac aaacattgga tttaacgaat gtcatggact ttattttaag
    13201 tgatgatcct caatttatgg cacaagccgg cagaaacgat caggttaatt atttgccgac
    13261 aaccaaattt aaaatgaaag taaacaaaga agcgataatt agtaacaatg tagtaccgga
    13321 gcaatttaaa gataaagtag tagacgaaat ggtttgggat ttgggtaaaa accgcataat
    13381 caaatttgat ttagccttat tggccatggt ggctgcccaa gactggagtc gtcctatttg
    13441 tttttccaat accgttgaag aaggctatta tcaggggtta ggtaaatatt tagtacaaga
    13501 aggagaagtg ttgcgtttag tgccatgttt gttccttgag aatcaaaaag ggtattacac
    13561 gatgaatact caacgttctt atgatatcgt gatgaatgat tttaaatacg gtggcttggg
    13621 cgacagagag atgtttgttg acgaaaattc agctcgtatt atgaacacct taaaaagcgt
    13681 tcactttggt attgctgatg atttagtaag aaatggtcgt caggcagatg ccgtaaaagt
    13741 gctggacaga gccaaaaagg aattccagta tgtaaatgca ccatattatg cacctaacaa
    13801 cagattcttt aatatcttaa ccgtgcaatg gatagatttg tattacagag ccggagcacc
    13861 tgcaaatgca aaatccatca aagagcttta tattaaagat cttaaagact gcttgcgttt
    13921 ctataattta ccgaatgact atgccgcatt gtatgcaaat gagaagaaat cggcggaaga
    13981 tatcgtaaaa cgaatggaaa tgttggctat acaatacaaa gacaatgaat ttatggctga
    14041 attaaataaa gctttcccga ctttggtgca aaagagtacg attgatttaa ctcagcaggt
    14101 acccaatcag attttcagat aattgtttga ttattaaata aaatcattac ttttatccca
    14161 ctaaaaataa tattatggga atgttacaag aattcaaaga atttgccatg aaaggcaatc
    14221 tgatcgacat ggctgtcggt gtggtaatgg gtggcgcttt tggaaaggtc gtgtctgcat
    14281 ttattgacgg catggtaatg cctgctatcg gtatgatttc gggtggcgtt gattttaacg
    14341 acaaggtgtt cgtacttaaa aaagctgttg cggcttctgc tgacggttca gttgcagcca
    14401 caccggaagt tgctatcaaa tatggtgctt tcatttcgca agtgattaca tttttagtgg
    14461 ttgcatttgt tgtctttatg attatcaagg cgattaacaa aatgaaaaaa gaagaagctg
    14521 ctgctacacc tcctgcacct cctgcagaag aagtgttact aacagaaatc agagatttgc
    14581 taaagaaata atgtatctca tgtaaaaaca ttgagggcgc gccacaggcg cgccctttct
    14641 ttttattgta gaatttagta ctaaatctgt aacactaaag tcttctgtga acctgtaatc
    14701 atgctaatct tcaatattga gaattaatga gtaataactt aagaaaaatt aggcgccagt
    14761 tcgtggtgct ctgtatcgta aaaattattg atatgttgca cggcttcttc tacatcatct
    14821 gttattagga ataaatttaa gtcttcctcg tttatattgt gctgattgcc cagtacctcc
    14881 gattttatcc agtctatcaa gcccgaccag aatgctttgc ccattaatac gataggaact
    14941 ctgtcgattt tttttgtttg taccaaagtc attacttcaa aaagctcatc aaatgtgcca
    15001 aagccaccgg gcagatatac gaaaccctgt gcgtatttta caaatactac ttttcgtaca
    15061 aagaagtaat gatgtgtgat gaccttatcg tgatcgatat gcggattatg tccctgctca
    15121 aaaggcaatt caatatttaa tcctaccgac tttccgtctt tattttgtgc tcccagattc
    15181 cctgcttcca taataccagg accacctccg gtaataacgc catagccttc ttctaccaaa
    15241 cgctctgcca ccttggtcgc catgctatag taagggtttt cgggtttggt acgtgcagaa
    15301 ccaaatatgg aaatacaagg accgattttg tccatctttt cataggcatc tacaaattca
    15361 ctcataatgc gaaacatact ccaggagttg gatgcattga cttgtgtcca gttgcgctgg
    15421 cgataaggtc ttttcttttt aaaatcttct aatgacatgt gctatagtta aaagttattc
    15481 agttgctttt cagagaaaaa cagcctgcta agatagtaat tttttatttg gaggagatta
    15541 ggctatcatc taatatgttg tcaccttgtt tacttgagcc cgatatcaga tataaaccta
    15601 aaaacgtcag caatccattc catagaagca tctccggccc aaatttgaaa tcaggaatga
    15661 gcttagtgga attcatatct agcagaaaac aaataaccgg cgaagcaata cagatgtacg
    15721 gggtgagttt atctttgata gtacgcttgg ttaaaatgcc gaacataaac aagcctaaca
    15781 gtggtccata agtatagctg gctattttca gtaaggtatc aataatggct tctttattca
    15841 ggattttaaa tcctaaaaca aataacaaga aaacaaacgc aaaggtgaaa tgcactttta
    15901 atctagtttt tgttctgtct tcttcgctta gattttcatt ttggttaata cccaaaatat
    15961 caatacaaaa tgaggccgtg agcgctgtaa gtgcaccatc agcactcgga aacagtgccg
    16021 aaatcaaacc gatgataaat aagatggaaa taaaaggagt aagataattg agggcaatgg
    16081 taggaaataa atcatcgctg tttttagcaa cgaaaatatg cgatactgca tcccataatt
    16141 ggaattttcc atctgcgata tgagcgatat taagttgctc accgtataaa aacaagacac
    16201 caccaagcac cagaaaaatg agattaacag ccaccaaaat aaaactaaag gtgaccatat
    16261 tcttttgcgc atcgccgagg tttttacaac tgatgttttt ttgcatcatt tcctgatcca
    16321 atccggtcat ggtgatggtg ataaacatgc cgccaataat ttgttttata aagaaattgg
    16381 gtttcaacca atctgtcata aatatttgcg tatatccctt ttcttgcaac gcactcatca
    16441 tgccacccac gctcatcccc aactggtttt ttatatacca tacgcaagtc accagtgcca
    16501 gcagcataaa agttgtttgc agcatatctg tccagacaat gctttttaca ccacccttaa
    16561 aagtatatag caatatcatg gtaagtataa caaatgccgc taaagtaaaa taacccatac
    16621 cctcgggcaa cagaaaaatc tgcaagacat tgatcactaa atacaagcga agtgtagcac
    16681 ctaaggtcct ggataagata aaaaaggctg cacctgtttt atacgaaata tcaccaaagc
    16741 gatgttgaag gtaggtatag atagaagtga ggttgagacg gtaataaagc ggcaataata
    16801 tatatgccac tacaaaatag ccgattaaat aacccaatac tacctgaaaa taagcgaaat
    16861 gtgctgtgcc tactttgccg ggcacggaga taaatgtaac tcccgataaa gaagttccta
    16921 ccataccata cgccacgata taccaacgcg atgaacgtcc gcctacgaaa taatccttgt
    16981 tgctggcatt gcgcgaagta taccaagcaa ttacaaacaa gaaaatgaaa taagcaataa
    17041 gacaaacgaa gattaaactg gtgttcatgc tgattttttt gatttgacag tctaaaaata
    17101 gatttattat ttcataaatc tgctatatca agcaattgtg gttgctcatc gtcagaggcg
    17161 actttatgct aaaatttcaa aaagagaaat cctatcttta gcccatgcaa tttccatcca
    17221 gacttattga agatgccgta aacgcatttg ccaagctacc gggcattggc aaaaaatctg
    17281 cattacggat ggtagtgtat ttgctgcaaa aagaaactgc cgatacggag caaattgcac
    17341 aggctatctt gcgtatgcgc aaggagatac aattttgcga ggtttgtcat aatgtatcgg
    17401 atgcggcaat ttgttcggta tgcggcaacc cgcaccgcga taaaagtaca atatgcattg
    17461 tggagaattt cagagatatt attgccatcg aaaacacaaa gaattacaac ggggtatatc
    17521 atgttttaga gggtttaatt tctcccatcg acggcatagg accggacaaa ctgaatattg
    17581 ccaaactgat ggagcgcgta caaagcggtg aggtaaaaga aatcatcatg gcgctcaacc
    17641 caactttaga cggcgatacg actatctttt atttatcgaa acaattagcg gcaatgccca
    17701 taaaaatcac caccatagca cgcggtatat catttggcgg cgaactcgaa tacgtggatg
    17761 aaatcacctt gtcgagaagt atacaagcgc gcttgcccta tgagcggtat atgtcgagat
    17821 gatgaaaata atacttacag caaattatac cctaagctca agtgttaaat gcagtttttt
    17881 ggggtaaaaa caatattttt tcaacttcaa ttacaaaaca atcagcatta ctgcatgaac
    17941 aatttatttg taaggaatat ttactaccat gttttactgc ttgaaaacgt gtttttaatc
    18001 ccaataaaca aataggaaaa actgagtatg aaatctgtaa ttggtgcaaa gtaccaacgg
    18061 gtaaacaagc ttaaatgatt gtttaattga tgataaacgc caagcaaaac taaagagtaa
    18121 agcaagataa aactacttac attttgaaag tctacaggta gcaaacaaag tgttatcgcc
    18181 cagatagtca tttttgtaaa tccaaaaaga aacaaaagta atttgtgtga aggcttgtat
    18241 tgccaactgc tctgtgtgtg cctgctcttc tgcttcaacc aggatgtcca agttgttttt
    18301 ggatggctaa acgtaaaagc ctcagggttt aaacaaacat ccacgttgtt tttgttggct
    18361 aattgttgaa ttgctaaatc atcatcacca ctgactatag catagtcttt atcgctccag
    18421 tcaagctgct gcaataaatc tttactgtaa gcaacattgc gtcctacaga catatatgca
    18481 ttgcccaaag cagcaaaacc cagatattgc aatgccgttt gtgcagtttc atacgctatc
    18541 aatttattga gcaagccctt tcttttttca tataaaccaa taccgagtac aatatctgtt
    18601 ttatcgttaa aatgtgccac catctccttc acccagtttt tagaagccgg ataacaatcc
    18661 gcatctgtaa acaagataat ttctccgttt gctgatttta taccttgaag caaagcatac
    18721 tttttaccca atccgcgttt ttcttccgaa ggaataaata ctgtttttat cttgtttcca
    18781 aaagtttgaa gtacagcttg tgtattgtca gtggagccgt catcgacaat tagaatctcc
    18841 acatcagggt aatcttgttc tatgattttc ggaagatgct gtttcaaatt aaactcttcg
    18901 tttttgctac aaattacaat actcaccttt ggaaatatcg aagatggagc cttatcgggc
    18961 gttttggttt taccaataag agtaaaatac agtacaatta aataaatcgt ttgaaacacg
    19021 atacacgaac ctaatacaat caaccaaaca gacattttat aaaaatagag attttatctt
    19081 atttttgact tttcattcag gttttgacat ttagcatttt acatacagac aaccaatcat
    19141 ctgcccgaac tggcatgatt actactgaac acggcaacat agaaacaccg attttcatgc
    19201 ctgtaggaac acaggctact gttaaagctg ttcagttttc agagttggaa aatgagataa
    19261 aagcgcaaat tatcttggga aatacttacc atctttattt gcgtcccggt ttagatattt
    19321 tgcgggctgc cggtggtttg catgccttta acacctggca aaagcccatt ttgacggaca
    19381 gcggaggcta tcaggtatat tcactcagcc atcggcgcaa gctgacggag gaaggagtag
    19441 tttttcaatc gcatattgat ggcagtaagc atttgtttac gccggaaaac gtaatggaca
    19501 cacagcgtat tatcggagct gattttatca tggcttttga tgagtgtacc ccctatcctt
    19561 gtgagtatgc ctatgccagg aaatcgttga agattaccca cgactggctg ctgcgttgta
    19621 tccgtcgttt tgatgcgaca gagcctttat atggttatga gcagacattg ttgcccattg
    19681 tgcagggtag tgtctataaa gacttgcgcg ccgaatctgc tgaatttatt gcaaaccaaa
    19741 acagatcggc aaatgcgata ggcgggctcg ccgtaggcga gcccgcagaa atcatgtacg
    19801 aaatgacggc tttggtcaat tccattttgc ctaaagacaa accacgctac ctgatggggg
    19861 taggcacgcc tgccaacttg ctcgaaagta tagcattggg tgtagatatg tttgattgtg
    19921 tgatgcccac ccgcaacgcc cggcatgggt tgctttttac ttccgaaggt atcatcaata
    19981 ttaaaaatca gaaatggaaa aatgattttt cgccaattga tgctactatc cccgcctcaa
    20041 caagccggaa gcacacaaaa gcctatctca ggcatctgtt tgtggtagag gaatatttgg
    20101 gtgctcagct ggcaagtatt cagaatcttt ctttttactt gtggctgatg caagaagcca
    20161 aaaacaggat aaaagacggc accttcagta catggaaaga tgtagcgatc cgaaaattta
    20221 tgacgagatt ataattcttg aacatagcat tttggctt
//
LOCUS       DAOQII010000010        19557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1270_length_19557_cov_5.374064, whole genome shotgun sequence.
ACCESSION   DAOQII010000010 DAOQII010000000
VERSION     DAOQII010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19557
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_19557_cov_5.374064"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..708)
                     /locus_tag="PLF48_01055"
     CDS             complement(61..708)
                     /locus_tag="PLF48_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF47937.1"
                     /translation="MRLTKLKYPVSVLSMFFAFVMLSAFTIQKTTQQPATSAGVISVK
                     SLNLAKSTVVWIGKKITGQHTGTVNISSGTITTKNGILGGGEFTIDMNSLKCTDIQDS
                     GYNAKLINHLKSEDFFNIAQYPVATLKITKVLKIKNKSNAYNLTGNLSIKGITKAISF
                     PATFKKAGNAYEAVATISVDRTKWDIKYGSANFFEGLGDKAIKNEFVLNVNIVTN"
     gene            1026..2099
                     /gene="prfA"
                     /locus_tag="PLF48_01060"
     CDS             1026..2099
                     /gene="prfA"
                     /locus_tag="PLF48_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNF47938.1"
                     /translation="MLDKLEAIFIRYKDLEQQLTDPAVVGDLARLKKTAKEYKDLEPI
                     IKAYHRYSNLLSNIEFNKAIINEEKDEEMRDMAKAELAELEEELPKLEDEIRYLLIPK
                     DPEDDKNAILEIRAGTGGDEASIFAGDLYRMYMRYCTLKGWQTEVMNENPGTSGGYKE
                     IVIEVSGDGVYGILKYESGVHRVQRVPETESQGRVHTSAASVAVLPEAEEVDVEIKES
                     DIRRDVFRASGAGGQHVNRTESAVRLTHIPTGTVAECQDGRSQIKNYESALKVLRSRI
                     YEKAVMEHQEAIAKSRKTMVSTGDRSAKIRTYNWPQGRVTDHRINLTLYNLGEIVNGD
                     LDPIIEKLVFEENAEKLKSGGLT"
     gene            complement(2137..3342)
                     /locus_tag="PLF48_01065"
     CDS             complement(2137..3342)
                     /locus_tag="PLF48_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF47939.1"
                     /translation="MYSNIKLKRNIYSTEEHLMLQKMCQQFIEKEITPYHAQWEEEGM
                     VSREVWTKAGEAGLLCLDVPEEYGGPGLDFSFCALITEEISKAGYTGPGFFLHSDIVA
                     PYLIDFGSEAQKMHWLPKMVSGEVITAIGMTEPSCGSDLQALKTTATDQGDFWEINGQ
                     KTFITNGYMSEMCIIAARTIVNGQDKGISLFIVEAEREGFSKGKPFKKVGMKAQDTCE
                     LFFDNVKIPKKNILGEIGKGFIYMMQKLARERMSVALMSVGGAEGALEQTIEYVSSRY
                     AFKQPIAGFQNTQFKMAEMATQLQIHQTFVDRCVELLCEGELSAETASMAKSSCTDMH
                     QQLVDECMQLHGGYGYMWEYYIARAYADNRVARIYAGTNEVMKLIIARGLFRELFEAQ
                     KRQKLQGAV"
     gene            3397..4155
                     /locus_tag="PLF48_01070"
     CDS             3397..4155
                     /locus_tag="PLF48_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF47940.1"
                     /translation="MDAKSRFSDRVEHYIKYRPGYPRKELLVLLEKEIRLTPKMNIAD
                     IGSGTGISARIFLENGNTVYGIEPNEPMRKAAEGWLHEFPNFHSLHGSSEATNLPPHS
                     IDLIVCAQAFHWFNPQLTKAEFLRIAHPDAYLCLIWNDRKRDAPFQQDYESLIQQFAF
                     DYNEISHRNITSERIQDFFAPSEFKEVILYNEQIFDLESLIGRIISSSYMPNRNSPEY
                     NAMEKAIIALFEKHEKKGEVVFAYDTLVYIGRIK"
     gene            complement(4159..7239)
                     /locus_tag="PLF48_01075"
     CDS             complement(4159..7239)
                     /locus_tag="PLF48_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF47941.1"
                     /translation="MRKLQLLSLFILLFIFKNIKADHLMGGEMTYQCLGNNNYEINLI
                     IYRNCNSTNTNLDPDAYITVFNAQSNFIYKTATIPLTNQSHIRIPPATPCFTPPSPSQ
                     VCMDKLVYSGTVNLPASTTGYKLVYQRCCRNPAITNLFNAGDIGSTYEIIIPPSTITT
                     CNNTPVFNSTTPAFMCVNQYSYQDFSATDADGDLLVYSLCAPKLGADLNDPRPTQASR
                     PPYADVPYNLQFSETEPLGPGSLISINAATGLLSMKPATSGYFTVGICVAEYRNGVLL
                     NTVIRDVQFNITNCSVTQSIPSVNLNASNAITQTSDTSYMSCNGLTVSFLNNSNNATT
                     SFWDFGVSTLTNDTSIIKTPTYTFPDTGVYHVTLIINKGQTCGDTATIEVRVLKELRG
                     DFSYLNPICANATELLQDLSTSFYNDINGWKWYFGDGDSSILQNPTHSYTEAGNYSIN
                     LVATTQRGCKVTATKTLTVTPGPAPNFIVKQVCKNRLTYFTNQSTIQSGTIIKYIWDF
                     GNGIKDSVNTNPSTVYPSDGTYTITLTAVSDNGCRTSTTKTIEIRDNFKVDFTATSVC
                     ERNSPVFNNTSTGYGNYLWRFDDGTTSTATNPTHIFTTAGTYAITLTASHAICASDSI
                     TKSVIIKPNPSFTLNKNISFCEQTTFNLTLPGTYDSLRWNTNQTANSIIIDGSINPIK
                     VTAYLNGCIKSDSTKAIIYPKPNAEFLTDKFCANKPTAFLNTSYVIGDNIAQNNWTLT
                     NGISTITSVQKEPTLNLNQLTPYQVQLIVTTTHGCKDTFRNTLSLLDTFSVGMKIPDA
                     VCIKNSNNFADSSVGNNAFYSWNFGDGQIANTASATHTYLTPGIYTFTHIVRNQSCGE
                     DSISKQILVKDLPVVDLGDDIIMCPGAITAVLLPGNFDSVIWNNGSHTNPANYDGNYP
                     VISVTAYQLGCYNSDLVDVILNCDVFIPTAFSPNGDGINDYFNFLSHSLKTFTLKVYN
                     RWGELVFETGSYTNSWNGTYKGKPCDSDDYVYIAYGIKNDNKPFYMKGTVTLLK"
     gene            complement(7317..9644)
                     /locus_tag="PLF48_01080"
     CDS             complement(7317..9644)
                     /locus_tag="PLF48_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF47942.1"
                     /translation="MKSFSKYILFLITLPLFFYFLSEKNVLAQNAPLGTWRAHVPLQS
                     ATSVCKSKENIYAACSNGIVSVNMDNHYMETYTKVNGLAEVFTNQVGYDTSLSVLVIA
                     YENSNIDLIQNGKITNIPYLKNTVISGDKKIYSIFCKDGRAYLGLGFGVMILNLDKKE
                     ISETYTFSDLAGFIRVNALTVFNNEIFCATNMGIISGRMAANVNLLNFNNWTKHSNGI
                     PQNEAFSITTYGGNVYSGLDNSLYRFDGTTWNNLLTQSNWQTIHLNNSNNGLQAVQYN
                     TSIGQSKIGRWNGSSFDFLPDDFYIARPLQTVSDYNNHIWSADSFRGLVQENNNTFSA
                     FIPNAPFSRACREMDYMNGTIYSTSSAINNGWAPNGLPEARYFSICKDYDWTTHSQYT
                     DAILNDVNQIAVVKTLPSQNKVFFGAAEFSQGGIIEYSLDDNSIKIEKNAPGVPESFR
                     ITGAEADEYDNVWFSNAYSHAPLICRKADGSYRYFNSGYLSGELVKDVVVDDYNQIWV
                     AKESGSGGLVMLNYGNDIDDQSDDQYFNFTTGQGYGNLPVTNVICLAKDKQGDIWLGT
                     TKGIARITCAAYVTDNACEAEQICINRNDGSGFCDNLLEDEVVNCIKVDNANRKWIGT
                     ANGLFLVSADGLETIYKFTEDNSPLLSNVVRGITINEENGDVFIATNKGICSYRGDAT
                     TTTASFSEAFVYPNPVEHDYFGPIAIRGIPDNCTVKITDFSGNLVYQTTATGGQAVWD
                     GNLTNGKRAATGVYFALCLGSDKKQKAKLKFVLFN"
     gene            9916..13239
                     /gene="ileS"
                     /locus_tag="PLF48_01085"
     CDS             9916..13239
                     /gene="ileS"
                     /locus_tag="PLF48_01085"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNF47943.1"
                     /translation="MAFKEIKHITQSDIEKQILDFWQTDNTFHNSITTREGNPDFVFY
                     EGPPSANGMPGIHHVLSRSLKDIFCRYKTLKGYKVNRKGGWDTHGLPVELQVEKKLNI
                     TKKDIGTKISVAEYNKECREEVMKFKNVWDDLTQKMGYWVDLEHPYITFENAYIESLW
                     WLLQQLYDKGLLYKGLKIQPYSPAAGTGLSSHELNLPGCYREVKDTTCVAQFKFIPQS
                     LVQSPQLKAVIEQNNIADTLYFLAWTTTPWTLPSNTALCVGPNIDYVLLETFNPYTNI
                     QEQLILAKDLVGNYFQIDNENKAFSEYKNDGKNLPYRILSECKGNDLVGIRYEQLLPF
                     ESNSKAHIDGDAFRVISGDFVTTSDGTGIVHIAPSFGADDMKVAAENGIGSLTLVDKE
                     GKFIDGTGEFSGRYVKDYKNEPNYVSVDIDICVKLKKENKAFKVEKYEHSYPHCWRTD
                     KPIIYYPLDSWFIRVTAVKDRMIELNKTINWKPESTGTGRFGQWLENLVDWNLSRSRY
                     WGTPLPIWRTEDGTEEICIGSIEQLQGEISKSLEAGFLKENPFKNGLDLHKPYVDDVI
                     LVSPSGKPMHRETDLIDVWFDSGAMPYAQFHYPFENKDIIDEKKGFPADYIAEGVDQT
                     RGWFYTLHAIAVMLFDSVAYKNVISNGLVLDKNGNKMSKRWGNAVDPFETIGKYGADV
                     TRWYMIANSQPWDNLKFDTEGIEETGRKLFNTLSNTYSFFALYANIDGFENATAQIPV
                     ENRPEIDRWILSLLNTLVKNTEQNLDDYEPTRAAREIQEFVNDHLSNWYVRLSRRRFW
                     KTDVETDKIAAYQTLYECLLKLTQLMSPIAPFYADFMYQNLTCKSESVHLSLFPTVNE
                     HLIDKQLEERMELAQNISSMVLSLRKKENIKVRQPLQKILIPAIDKNQIEQIDRVKEL
                     ILSEVNVKEIAYISDTSGIVSKKVKPNFKLLGKKLGAKMKAVSEIIQQFDNQQIAELE
                     KNGSVSIQFEIENFPILLEEVEIVSDDIQGWLVASNNGMTVALDSTITESLKNEGFAR
                     EIVNKIQNERKEADFQVTDKIVLYVQNHLVLNEVLKSYKEYICNETLAQDIVFTDIEE
                     RAKDFDINGELLKLFILKL"
     gene            13243..14277
                     /locus_tag="PLF48_01090"
     CDS             13243..14277
                     /locus_tag="PLF48_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNF47944.1"
                     /translation="MGKVIKKTKTVPQKTNAKPAAKKAAAKPVVKPAAKKATKPVAKT
                     VAKPAKTAAKSGKPVAKTVEKAKTKTIAKPIAKKTGVKVATKKAATKAVAKPTAKKAT
                     AKTEVKKVAVKATKSKPVTKKVTKPIAKSTPAKKAPAAKTVAKPVAKVAAKSVKKAAE
                     VKKTAPLKPVKKAVAAKVEKTKSVAKKAVIVKAEKQVRKEKVTQIVHSHHQHIPHHLH
                     TEPKPFEVSMVRYSDADLNLFRAIIQKKIAKAKEEIDYYNEQIRSSSESDTQFASIDD
                     GSMTSERENMNQIVSRQMKLISHLESALVRIENKTYGICRETGKLIPKERLMVVPHAT
                     LSVEGKKLQK"
     gene            14377..15006
                     /locus_tag="PLF48_01095"
     CDS             14377..15006
                     /locus_tag="PLF48_01095"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNF47945.1"
                     /translation="MTKKNIAILTVVLSLLIDQASKIWVKLSMTLGESIPYIGDWARI
                     QFVENEGMAFGMSFGAGLGKFLLTMFRIVAVFFITYYLAKIVKEKNSSKGFVFALALI
                     LAGAVGNIIDSIFYGKIFTDSNGRIATLFPQQGYAGWLHGKVVDMLYFPILQPGVIKW
                     PSWIPGVGGQWIEFFPFIFNVSDACITVGVILILLFQKRFLQENNTQSN"
     gene            complement(15056..15895)
                     /locus_tag="PLF48_01100"
     CDS             complement(15056..15895)
                     /locus_tag="PLF48_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNF47946.1"
                     /translation="MQTSELQNIATQVRRDIVRMVHQCKSGHPGGSLGCADFLTALYF
                     EIMDIKIPFSMDGKNEDLFFLSNGHISPLFYSLLARRGYFDVKELGTFRHLNSRLQGH
                     PTTHEGLPGIRIASGSLGQGISVAIGAALSKKINNDDKLVFCLTGDGELQEGQIWEAL
                     MFAAAKKVDNLIVTVDWNDAQIDGSTQQVLDLGDLHTKFQSFGWETFDMKGNDMEDVV
                     RVLKQAKAASKNGKPVAILMRTQMGYGVDFMMGTHKWHGSAPNDEQKDKALAQLKETL
                     GDY"
     gene            complement(15947..18616)
                     /locus_tag="PLF48_01105"
     CDS             complement(15947..18616)
                     /locus_tag="PLF48_01105"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNF47947.1"
                     /translation="MKKFFKGLLLTFLVIIVAAFIIPFFFKDKLIGIVTQQAENYIDA
                     KPHVGKVDLSILKNIQNFPNIALTLTDVDIINKAPFEGDTLLHLGDAKISLDLMSVIK
                     GSNYKIEAITLKDVILNAIVNKDSMTNWNIIKSSSETDTSKSSPFSLALKKITLDNIN
                     LSYNDFTTGNKLNIQNLNHDGKGDFTKEILDYTSTTEIAKAYFAQGLITYLKEAKIKL
                     DSKLNIDQPAKKYSFKDNKLSLNDLGLLFTGFVQQQANSATNMDIEFKADKTDFKSIL
                     SLIPAIYAKDFNKIKTSGKLQLDGIAKGIMQGKSYPQFALNLNVKDGFFQYPDLPTAV
                     KDIAIDAHVKNPGGTLDNTVVNIPDFHINIANEPIDGSLNIATPISNPDIDLAAKGKL
                     NLADVQKFYPSDDIKTLKGNANLDLKVRARQSDIVAKNYQNILASGNIAATGIEYRSK
                     DVPKPISVSNLLLNFTPQYVAVDQCIAKIGSSDFDIKGKLDNVIGYVLMKGAKLGGNI
                     NIVSNKINANEFLPDSSSANKTKSQKAQEVMRIPDNIDLNGTASVKELLYDKLNIKDM
                     VGNITLKDEQLNLNNLSANLLGGSAIVSGFYNTKTDIPTAKINYHITSFDIQQLYKYS
                     GVAKNITPIMQYISGTFSSNMDINTTLNPDLSPDLKTINGTAGVNIPLAIAQNVPVLN
                     KIKEMTQLQQLDKLQFQNVNIKTTFVNGRMMVDPFTMKANNLSMTVGGSQGMDQTVDY
                     AVAIDVPWKELGKASSFATGLLAKNPIPQLNGMVPEIIRLNLKVGGTLKDPKITMGKP
                     DGKVGGGTMKDVVKEQVQQQVQQIKEEVKTQAKQTLDTLKQTAKEQAKNKLNEILTGQ
                     KDTSTNSNPAQNLKENVKENVIDKVKNKFPWPR"
     gene            complement(18765..>19557)
                     /locus_tag="PLF48_01110"
     CDS             complement(18765..>19557)
                     /locus_tag="PLF48_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47948.1"
                     /translation="LMRCGSDVNSRVQPLQQSIRQSNQIVEDNVNGRLSGSPDLIESS
                     NFYYNSDGTLQKIIVLNDTGNSGVVLKKVEFEYHSDYVYARTYRLGAGELNVSFFFNN
                     KKQITKLVDDAGNGLRFTYTNDLLTSFRDSSGVAILDYINFEYDGNNNLLKYELSING
                     SPPVGRAELTYGSDTIIPDLDTRFFNKGVNFIYLGGLNVIRKCGLNYGIDNRNVLLKR
                     VETNLMTNTTIDTYDFGYIYDDQNRIVKRNIRRSGEDTLFYRYKY"
BASE COUNT         6160 a         3641 c         3758 g         5998 t
ORIGIN      
        1 cggacaagcc acaaaaacca taaaactatg aaaaacccaa ttgcctctcg gcaataatct
       61 ttaattcgtt actatgttta cgttcagtac aaactcattc tttattgcct tatctccaag
      121 tccttcaaaa aagtttgccg atccgtattt aatatcccac ttcgttctgt ccacactaat
      181 tgttgccaca gcttcataag catttcctgc tttcttaaac gttgcaggaa atgaaattgc
      241 tttcgtaata cctttgatgc ttaaattgcc tgttaggttg tatgcgtttg atttattttt
      301 aatctttaac actttggtaa tcttgagtgt tgcaaccgga tactgagcta tattaaaaaa
      361 gtcctcagat ttaaggtgat taatcaattt tgcattatag ccggagtctt gtatatccgt
      421 gcattttaat gagttcatat caatagtgaa ttcaccaccg cctaaaatgc catttttagt
      481 tgtaattgtg ccggaactga tatttacagt accagtatgt tgtcctgtaa ttttttttcc
      541 tatccaaact acagtacttt ttgcaagatt aagtgattta acagaaatta ccccagctga
      601 agttgcaggt tgctgtgttg ttttctgaat tgtaaatgct gaaagcatca cgaatgcaaa
      661 aaacatggaa agaacagata cagggtattt aagttttgtt aatctcattt caaaaaaata
      721 ttagcaaaca ttgtagcaac aaatatcaga ccaaagatat tgcatattgg tcaattttgc
      781 atgttaaaaa ccctaaattt ttctcaaaag gcttattatt attcattttt atgttttttt
      841 tgatacatat caaaagagtg aaaggatgct tctgtttaaa tatttgaatg aaataaacgt
      901 aaactattta agtaaactta gtttaaatgc cggaagtctt tgtttgatgt atccataatt
      961 tgagatctgg tggttaacga ttagtaaata attataattt tgaaaaagat tacctttgcg
     1021 ccgccatgtt agataaactc gaagccatat tcattcgtta caaagatttg gaacagcaac
     1081 ttaccgatcc ggctgtggta ggtgatttgg cacgtcttaa aaaaacagct aaagaatata
     1141 aagaccttga gccgattatt aaggcttacc accgctatag caatctgtta tccaacatcg
     1201 aatttaataa ggccatcata aatgaagaga aagatgagga aatgcgtgac atggctaaag
     1261 ccgaacttgc agaactagaa gaagaattgc ccaaactgga agatgaaatc aggtatttac
     1321 tgattcctaa agatcccgaa gatgataaaa atgccatttt agaaatacga gcaggcacag
     1381 gaggtgatga agccagcata ttcgccggcg atttataccg gatgtatatg cgctattgta
     1441 ctctgaaagg atggcaaaca gaagtgatga atgaaaaccc gggtacttct ggcggatata
     1501 aggaaattgt aattgaagta agtggtgatg gcgtttacgg catactgaaa tacgaaagtg
     1561 gtgtgcatcg tgtgcaacgt gttcccgaaa ccgaatcaca aggcagagtt catacttctg
     1621 ctgcatccgt agcggtattg ccggaagcgg aagaggtgga tgttgaaatt aaagaaagcg
     1681 atatcagacg agatgtattt agggcgagtg gtgccggcgg acaacacgta aacagaacgg
     1741 aatctgctgt gcggctaaca catataccaa ccggcacggt agcagaatgt caggacgggc
     1801 gctcacaaat aaaaaattac gaaagtgcat taaaagtatt gcgttcccgt atttatgaaa
     1861 aagcagtaat ggagcatcag gaagccattg caaaaagcag aaaaaccatg gtaagtacag
     1921 gagaccgcag tgcaaaaatt agaacataca actggccaca agggcgagtt accgatcacc
     1981 gcatcaatct tacattgtat aacttaggcg aaatagtaaa tggtgattta gacccgatta
     2041 tcgagaagct cgtatttgaa gaaaatgccg aaaagctcaa gagtggcggc ttaacataat
     2101 tttcattata atcagatggg atagttttca ggatagctaa actgcgcctt gcagtttttg
     2161 tcttttttgt gcttcgaaca attctctgaa taatcctctg gcaataatga gcttcatgac
     2221 ttcattagta cccgcatata tacgtgcaac tcgattgtcg gcatatgctc tggcaatgta
     2281 atattcccac atataaccat aaccgccgtg caattgcata cactcgtcta ctaattgctg
     2341 gtgcatgtcc gtacaagaac tttttgccat cgaagcagtt tcggcactga gttcgccttc
     2401 acacaataat tctacacaac gatcaacaaa tgtttgatga atctggagtt gtgtggccat
     2461 ttcggccatt ttgaattgag tattctggaa tccggcaata ggttgtttga atgcataacg
     2521 ggaagaaaca tattcaatag tttgctctaa agctccttcg gcaccgccca ccgacattaa
     2581 tgcaaccgac atgcgttctc tggctaattt ttgcatcata taaatgaagc cttttccaat
     2641 ttcacctaaa atatttttct tagggatttt tacattatca aaaaataact cacaagtatc
     2701 ttgtgctttc atgcccactt tcttaaacgg ctttcctttg gaaaatcctt cccgttccgc
     2761 ttctacgata aataagctga tgcctttatc ttgtccattc acaattgttc gggcagcgat
     2821 gatacacatc tcactcatat agccatttgt gataaaggtt ttttgaccgt ttatttccca
     2881 aaaatcaccc tgatctgttg ccgttgtttt caaggcttgc aaatcactgc cacagctggg
     2941 ttctgtcatg ccgatggctg taattacttc tccgcttacc atttttggca gccagtgcat
     3001 tttttgagcc tccgatccga aatcaatcaa atatggggca actatatcag aatgcaaaaa
     3061 gaaaccggga ccggtataac cagccttaga aatttcttca gtgattaatg cacaaaaaga
     3121 gaaatccaat cccggaccgc cgtattcttc aggaacatcc aaacaaagca aacccgcttc
     3181 tccggctttt gtccaaacct cgcgactcac catgccttct tcttcccatt gtgcatggta
     3241 gggagtaatt tctttttcga taaattgctg acacattttt tggagcatca aatgctcttc
     3301 tgtggagtaa atatttcttt ttaattttat attggaatac atgtaaaaat tgtttctgca
     3361 aaggtactta tttcgtaaca ttttttaatg accgttatgg atgcaaaatc ccgtttttca
     3421 gacagagtag agcattatat taaataccgc cctggctatc cccggaaaga attattggta
     3481 ttgctggaaa aagagattcg gttgactcca aaaatgaata ttgcagatat tggaagtgga
     3541 accggaattt cagctcgtat atttttggaa aatgggaata ccgtttatgg catcgagcct
     3601 aatgaaccca tgcggaaagc agcagaaggc tggctgcatg aatttccaaa ttttcacagt
     3661 ttacacggca gctctgaagc aacaaacctc ccgcctcatt cgatagattt aattgtatgc
     3721 gctcaggctt ttcactggtt taatccgcaa ctgactaaag cagaattttt gcgtatcgct
     3781 catcctgatg cttatttatg tttaatttgg aatgacagaa agcgtgatgc ccccttccag
     3841 caagattatg aatcgctgat tcagcagttt gcctttgatt acaacgagat atcacatcgc
     3901 aatattacaa gtgaacggat acaggatttt tttgctcctt ctgaatttaa agaagtaatc
     3961 ttgtataatg aacagatttt tgacttggaa agtttaatcg gcagaattat ttcatcttca
     4021 tatatgccca ataggaacag ccccgaatat aatgcaatgg aaaaagcaat aattgcctta
     4081 tttgaaaaac atgaaaagaa aggtgaggta gtctttgctt atgatacatt ggtttatata
     4141 ggacgcatta aataaatcct attttagcaa agtgacagtt cctttcatat aaaaaggttt
     4201 attgtcattt tttattccat aagcaatata gacataatca tctgagtcgc agggcttgcc
     4261 cttataagtg ccattccaac tgtttgtata gctcccagtc tcaaatacca gctcacccca
     4321 acggttgtaa actttcaagg taaaagtttt caggctatgt gataagaaat taaaataatc
     4381 gtttatgccg tcaccattag gagaaaacgc agtaggaata aatacatcac aatttaagat
     4441 gacatcaact aaatctgaat tataacaacc caactgataa gctgtaacac taataaccgg
     4501 atagttgcca tcataatttg ccggattggt atgagagccg ttattccata taacactatc
     4561 aaaattaccg ggcaataaga cagcagtaat agcaccggga cacataatga tatcatcgcc
     4621 aaggtctaca acaggtaggt ctttcactaa aatctgcttt gaaatactgt cctcaccaca
     4681 actttggttt cgtacgatgt gcgtaaaggt gtagattccg ggagtgagat aagtatgtgt
     4741 tgctgaagcc gtattagcga tttgcccgtc tccgaaattc catgaataaa atgcattatt
     4801 gccaacggaa ctgtctgcaa aattgttcga gtttttaatg caaaccgcat caggaatctt
     4861 catgcctaca gaaaatgtat ctaatagcga tagcgtgttg cggaaagtgt ctttacaccc
     4921 atgtgtggta gtcacgatga gctgaacctg atagggagtt agctgattaa gattaagggt
     4981 aggttctttt tgtactgatg ttattgttga aattccattg gtcagtgtcc agttattttg
     5041 ggcaatatta tcaccaatca cataagaggt atttaagaaa gcagtaggct tattggcgca
     5101 aaatttatca gtaaggaatt ctgcattggg ttttggatag ataattgcct tggtagaatc
     5161 agacttaata catccattta aatacgccgt tacttttatc gggttaatgc tgccgtcaat
     5221 gataatgcta tttgctgttt gattggtatt ccagcgcaat gagtcatatg tgccgggtaa
     5281 tgtcagattg aaagtagttt gttcacagaa tgaaatgttt ttattaagag taaatgaagg
     5341 attgggttta ataataacgg acttcgtaat actgtcacta gcacaaattg cgtgactggc
     5401 tgtaagtgta atagcataag ttccggcagt tgtgaaaata tgggtagggt ttgttgccgt
     5461 actggtagtg ccgtcatcaa aacgccatag ataattgccg tatccggtag aggtgttgtt
     5521 aaataccggg ctgtttcttt cacatacgga ggttgccgtg aaatccactt taaaattgtc
     5581 gcgaatctca atagtttttg ttgtagatgt gcgacaacca ttatctgata cggcagttaa
     5641 ggtaatggta tatgttccgt ccgatggata aacagtggag ggattggtat tgacagaatc
     5701 ttttatgcca tttccaaaat cccagatata cttgattata gtaccgcttt gtatggtaga
     5761 ttgattggta aaataagtta atctgttttt gcagacttgt tttacgataa aattgggcgc
     5821 aggaccagga gttacggtta aagtttttgt agcggttact ttacaacctc gctgggtggt
     5881 ggcaactaga ttaatactgt aatttcctgc ttctgtataa ctgtgagttg gattttgcaa
     5941 tatactggaa tcgccatcgc caaaatacca tttccatccg ttgatatcat tgtagaaaga
     6001 tgtagataaa tcctgtaaaa gttcagtagc attcgcacaa attggattca gataagaaaa
     6061 atctcctctc aattctttca atactctgac ttcaatcgta gcagtatctc cacacgtctg
     6121 acccttgtta attatcagtg taacatgata aacgccggta tcaggaaatg tgtaggtggg
     6181 tgtctttatg attgatgtat catttgtaag agtggatacg ccaaaatccc agaaggaggt
     6241 tgttgcatta ttactgttat tcaaaaaact aaccgtcaaa ccattacaac tcatatagct
     6301 ggtatcgctt gtctgtgtaa tggcatttga agcatttagg tttacagacg gaatagattg
     6361 agtaactgag caattggtaa tattgaattg tacatcgcga atgacggtat tcagtaatac
     6421 accatttcgg tattctgcaa cacaaattcc aacagtaaaa tagcctgatg tagcaggttt
     6481 catgcttaac aagccagttg cagcattaat gcttattaaa gaaccgggac ccagaggctc
     6541 tgtttcgctg aattgtaaat tgtatggtac gtctgcataa ggaggtcgag acgcttgagt
     6601 tggtcttgga tcattcaaat cagcaccgag tttgggtgca caaagtgaat atactaataa
     6661 atcaccatca gcatctgttg cagaaaaatc ctgataggaa tactgattga cacacataaa
     6721 ggcaggagta gtagaattga atacaggagt attattgcac gtagtaatgg tagaaggtgg
     6781 aatgattatt tcgtaagtac tgccaatatc accggcatta aaaagattag taatagccgg
     6841 attacgacaa catcgctgat aaaccaactt gtatccggta gtacttgcag gcaggttgac
     6901 cgttccgcta taaaccagct tatccataca tacctgagac ggcgatggag gagtaaaaca
     6961 gggtgtagcc ggcggaatgc gaatatgact ttggttggta agtggaatgg ttgcggtttt
     7021 atagataaaa ttgctttgtg cattaaatac tgtgatgtag gcatcaggat ctaagtttgt
     7081 gttcgtactg ttacaatttc gataaatgat taaattaatt tcataattat tattacccaa
     7141 gcactgatag gtcatttcac ctcccataag atggtcggct tttatgtttt taaatataaa
     7201 cagtaaaata aacaggctta aaagctgtag ctttctcatg gttcaaaaat aactaaaaat
     7261 aaacactctt tagcctgact ttaaagctat gttttttagt atttacttaa gataaatcag
     7321 ttaaataaaa caaacttaag tttagctttt tgtttcttgt ctgagcccaa acatagtgca
     7381 aaatatacgc ccgtagcagc gcgtttccca tttgtaagat tgccatccca aacagcctgc
     7441 ccacccgtgg ctgtagtctg ataaaccaaa ttgccggaaa aatctgtaat ttttacagta
     7501 caattatccg gaatacctct aattgctatg ggaccgaaat agtcatgttc aaccggattg
     7561 ggataaacaa atgcctctga aaatgatgcg gtagtggttg tagcatctcc tctgtatgaa
     7621 caaattccct tattggtagc aataaaaaca tcaccatttt cttcatttat ggtgatgcct
     7681 cttaccacat tagacaataa aggtgaattg tcttccgtaa atttataaat agtctctaat
     7741 ccgtcagcac ttaccaaaaa aagcccattg gccgtgccta tccatttgcg attggcatta
     7801 tctactttga tgcaattgac tacttcgtct tccaacaagt tatcacaaaa tccgcttccg
     7861 tcgttccggt ttatacaaat ttgctcggct tcacaagcgt tatcggttac ataagcggca
     7921 caggtaatgc gggcaattcc ctttgtagtg ccaagccaaa tatccccttg tttatcttta
     7981 gccaaacaaa ttacattggt aaccggcaaa ttgccataac cttgcccggt ggtaaaattg
     8041 aaatactgat catcactttg gtcatcaata tcatttccat aattcaacat aactaaacca
     8101 ccgctgccac tttctttagc cacccaaatc tggttgtagt catccactac cacatctttt
     8161 accagttctc cacttaaata acccgaatta aaataccgat agctgccgtc tgctttacga
     8221 caaattaatg gagcatgtga ataggcattg gaaaaccata cattgtcata ctcgtcagct
     8281 tcagcacctg taatacgaaa tgactcaggt actccgggtg catttttttc aatcttaata
     8341 ctattgtcat ctaaagaata ttctattatt ccaccttgac taaattcggc tgcgccaaaa
     8401 aaaactttgt tttgagatgg caatgttttc actactgcaa tttggttgac atcgtttaag
     8461 atagcgtccg tgtattgtga gtgtgttgtc cagtcataat ctttacaaat tgaaaaataa
     8521 cgggcttccg gcaaaccatt gggcgcccat ccattgttaa tggcagaaga tgtagagtaa
     8581 attgtgccat tcatatagtc catttcacgg catgctctgg aaaatggtgc attgggaata
     8641 aatgcactga aggtattatt gttttcctga actaagcctc tgaatgaatc agccgaccaa
     8701 atatgattat tgtaatcact tactgtttgc aatggtcggg cgatataaaa atcatccggc
     8761 aagaaatcga aactgctgcc gttccatctt ccaatcttac tttgtccgat tgaagtatta
     8821 tattgtactg cttgtaagcc attattgcta ttgttgaggt gtatggtttg ccaattgctt
     8881 tgcgtaagca gattgttcca tgtagtgccg tcaaaacgat agagagaatt atccaatccg
     8941 ctgtaaacat tacctccgta tgttgtgatt gaaaaagctt cgttttgtgg aatgccattg
     9001 ctgtgcttgg tccagttgtt gaaattcaac agatttacat ttgctgccat tctaccagaa
     9061 atgatgccca tattggtggc acaaaaaatt tcgttgttaa atacagtcag cgcattaact
     9121 ctaataaagc ctgcaagatc actgaaagta taggtttcac tgatttcttt tttatcgaga
     9181 tttaaaatca taactccaaa acccaaaccc aaataagctc tgccatcctt gcaaaaaatg
     9241 gaataaatct tcttgtcgcc agaaataaca gtgtttttca aataagggat attggtaatt
     9301 ttcccatttt gaattaaatc aatatttgaa ttttcataag caataactaa aacagaaagt
     9361 gatgtatcgt agcccacctg attggtaaat acttcagcta acccatttac tttggtataa
     9421 gtttccatgt aatgattgtc catgtttaca ctaacgatgc cattgctgca agctgcatat
     9481 atattttctt tactcttgca cacagatgtg gcactttgga gaggtacatg tgcacgccat
     9541 gttcctaatg gtgcattttg agccaaaaca tttttttcac ttaaaaagta aaaaaataat
     9601 ggcagtgtaa ttagaaaaag tatatattta ctgaaacttt tcattgtttt aaattttaat
     9661 ggttaatggc gggcagttta agtactgccc gttttctatt taaccagatt caaaactaag
     9721 aaaataatac tacataaggc ttgcttcttt ttacttaaat tcaagactaa ataaaaacaa
     9781 atattaaata tatgatttat ttcatttcta caatctattc aattcttttt caatatatta
     9841 atgtcctttt ttgtgttaaa ccccctgaaa tgactatatt tgcgcaaaat ttttaagaaa
     9901 ggtaattgca gtaagatggc atttaaagaa attaagcata tcacacagtc agatatagaa
     9961 aaacagatac tcgatttttg gcaaacggat aacactttcc acaatagtat tactacccga
    10021 gaaggtaatc ctgattttgt attttatgaa ggcccgccca gtgccaatgg tatgcccggt
    10081 attcaccatg ttttatcgcg ctcgcttaag gatattttct gccgatacaa aacgctcaaa
    10141 ggctacaagg taaaccgaaa aggtggctgg gatacgcacg gcctgcctgt ggaattgcaa
    10201 gtcgaaaaga aactgaatat caccaaaaaa gatataggta cgaaaatttc tgttgctgaa
    10261 tataataagg aatgcaggga agaagtaatg aagttcaaaa atgtgtggga cgaccttaca
    10321 caaaagatgg gctattgggt agacttggag catccttaca tcacatttga aaatgcatat
    10381 atcgaaagtt tgtggtggtt gctgcaacaa ctttacgata aaggactact gtacaaagga
    10441 ttgaaaattc aaccctactc accggctgca ggaacgggct taagttctca tgaactcaac
    10501 ttgccgggct gctatcgtga agtaaaagat accacttgtg tggctcagtt taagtttatc
    10561 ccacagtcct tagttcaaag tccacagctc aaagctgtta ttgagcaaaa caatatagca
    10621 gatactttat atttcttagc atggacaact acaccctgga ctttgccatc gaatactgca
    10681 ctatgtgtag gacctaatat tgattatgta ttgctagaaa catttaatcc ttataccaat
    10741 attcaggaac aacttatatt ggcgaaagat ttagttggaa attattttca aatagataat
    10801 gaaaacaaag cattttctga atataaaaac gatggcaaaa atctgccata caggatattg
    10861 agcgaatgta aaggaaatga tttagtaggt atcagatatg agcaattgct tccctttgag
    10921 tcgaacagca aagcacatat tgacggggat gcatttcggg taatttcagg tgattttgtt
    10981 actacttctg atggtacagg cattgtgcac atcgctccta gttttggtgc cgatgatatg
    11041 aaagtggccg cagaaaacgg catcggctcc ttgactttag tagacaagga aggaaaattt
    11101 atagacggga caggagagtt ttcgggcaga tacgtgaaag actacaagaa cgagccaaat
    11161 tatgtgtcgg tagatattga tatctgtgta aaactgaaaa aggaaaacaa ggcttttaaa
    11221 gtagaaaaat acgaacacag ctatcctcat tgctggcgaa cagacaaacc gattatctac
    11281 tatccactgg attcttggtt tattagagta acagccgtga aagacaggat gatagaatta
    11341 aataaaacta tcaactggaa gccggaaagt acgggaacgg gacgttttgg acaatggctg
    11401 gaaaatctgg tagactggaa tttatcccgt tcacgttatt ggggaactcc gttgcctata
    11461 tggcgaacag aagatggtac ggaagaaatc tgtattggat caatagaaca attgcaaggc
    11521 gaaatcagca aatccttaga agccggattt ctgaaagaaa atccatttaa aaacggactc
    11581 gatttgcaca aaccatatgt ggacgatgtg attttggttt cgccatccgg caagccgatg
    11641 catcgcgaaa cagacttaat tgatgtctgg tttgacagtg gcgcaatgcc ctatgcacaa
    11701 ttccactatc catttgaaaa taaagacatt atagacgaga agaaaggctt ccctgccgat
    11761 tatattgcag aaggtgttga ccaaacccga ggatggtttt ataccctgca tgcaatagcc
    11821 gttatgttat tcgattcggt ggcctataaa aatgtaattt caaacggctt ggtactggac
    11881 aaaaatggta ataaaatgtc caaacgctgg ggtaatgcgg ttgatccatt tgaaacgatt
    11941 ggtaagtacg gtgcagatgt aacacgctgg tacatgattg ctaattcgca gccatgggat
    12001 aatctgaaat ttgataccga aggaattgaa gaaacgggac gaaaattatt caataccttg
    12061 tcaaatacct attcattctt tgctttgtat gccaatattg atgggtttga aaatgctaca
    12121 gcacagattc cggtggaaaa ccgtcctgaa attgaccgct ggatattgtc tttgctaaat
    12181 actcttgtga aaaatacgga acaaaatctg gatgattatg agcccacaag agcagcacgt
    12241 gaaattcagg aatttgtaaa cgatcatcta agcaactggt atgtgcgatt gagccgtcgc
    12301 cgcttttgga aaacggatgt ggaaacggat aaaattgctg cctatcaaac attatacgag
    12361 tgtttactga agttaactca attaatgtcg ccgatagcac cattttatgc tgattttatg
    12421 tatcagaatt taacgtgcaa atcagagtct gtgcacttga gcttatttcc tactgtaaat
    12481 gaacatttga tagacaaaca actggaagag cgtatggaac tggcacaaaa tatatcttcc
    12541 atggtgctgt cactacgaaa aaaagaaaat attaaagtgc gtcagccctt acaaaaaata
    12601 cttattcctg ctattgacaa aaaccaaata gaacagatag atagggttaa agaattgatt
    12661 ttgtctgaag ttaatgtgaa agaaatagct tatatatctg atacatcagg cattgtttcc
    12721 aaaaaagtaa aaccgaattt taaattatta ggcaagaagc tgggagcaaa gatgaaggcg
    12781 gtatctgaaa tcattcagca gttcgacaat cagcaaatcg ccgagctgga gaaaaatggc
    12841 agtgtttcca tccaatttga aatagaaaac ttccccattt tgcttgagga agtagaaatt
    12901 gtatcggatg atatacaagg atggttggtt gcgagtaata atggcatgac tgttgctctg
    12961 gatagtacaa taacagaatc attaaaaaat gaaggtttcg cccgcgaaat tgtcaataaa
    13021 atacaaaatg aacgaaaaga agccgatttt caggttactg acaaaatcgt gttatatgtg
    13081 caaaaccatt tggtactcaa tgaagtacta aaatcatata aagaatatat ttgcaatgaa
    13141 actttagcac aggatattgt gtttacggat atagaagaaa gagctaaaga ctttgatatt
    13201 aacggagaac tattaaaatt attcattcta aaattgtaga agatgggtaa agtgataaaa
    13261 aaaacaaaaa ccgtccccca aaaaacgaat gctaaaccag ctgctaaaaa agctgctgct
    13321 aaaccggtag ttaaaccagc cgcaaaaaaa gctactaaac cagtagccaa aactgttgct
    13381 aagccggcta aaactgctgc aaaatcgggt aagcctgtgg ctaaaacagt ggagaaagca
    13441 aaaactaaaa caattgcaaa gccgattgca aaaaagacag gggttaaagt tgcaactaaa
    13501 aaggcggcaa ctaaagccgt tgcaaagccg acagccaaaa aggcgacagc gaaaactgaa
    13561 gtaaaaaaag tggcggtcaa agcaactaaa agcaaaccgg ttacaaaaaa agtaactaaa
    13621 ccgattgcaa agtcaacacc tgcaaaaaaa gcacctgcag ctaaaactgt tgctaaacca
    13681 gttgctaagg ttgcagccaa atcggtaaaa aaggcagctg aagtaaagaa aacagctccg
    13741 ttaaagccgg taaaaaaagc ggtagcagca aaagttgaaa aaacaaaaag tgttgcaaaa
    13801 aaggcagtga ttgttaaagc agaaaaacag gtaagaaaag aaaaagttac acaaattgta
    13861 cactcacatc atcagcatat tcctcatcat ttacatacgg agccaaaacc atttgaagta
    13921 tcaatggtga gatacagcga tgcagatttg aacttattca gagcaattat ccaaaagaaa
    13981 attgcaaaag caaaagaaga aattgattat tataacgaac aaatcagaag cagttcagaa
    14041 agcgacacgc aatttgcttc tattgatgat ggctccatga cttcagaacg cgaaaatatg
    14101 aaccaaattg tttctcgtca aatgaaatta atatcacatt tggaaagcgc cttggtgcgt
    14161 atagaaaata aaacatatgg tatttgtcgc gaaaccggaa aactgatacc taaagaaaga
    14221 cttatggtgg tgcctcatgc cacgttgagt gtggaaggta aaaaattaca aaagtaatct
    14281 cagcgcttag taacaagcag atcgttttta agagcggtat gtgattatca aatttcagga
    14341 ttggattaca atttacaagt tacgaatcat taaaaaatga ccaaaaaaaa cattgctatt
    14401 ctcacagttg tattgtcatt attgatagac caggcttcga agatttgggt aaaattaagt
    14461 atgacactcg gcgagagtat tccttatatc ggtgactggg cgcgcattca atttgtagaa
    14521 aacgaaggta tggcgttcgg tatgagtttc ggagcaggtt tgggcaaatt cttactcacg
    14581 atgtttcgca ttgtagccgt tttttttatt acctattatc tggcaaagat tgttaaagag
    14641 aaaaattcaa gcaaaggttt tgtctttgct ctggcgctca ttttagccgg tgcggtcgga
    14701 aatatcattg acagtatttt ttatggcaaa atatttacgg acagcaatgg cagaattgca
    14761 acactatttc cgcaacaagg ttatgccggc tggcttcatg gcaaagtagt agatatgctt
    14821 tatttcccaa ttctgcaacc cggtgtaatt aaatggccat cttggatacc cggtgtaggc
    14881 gggcaatgga tagagttttt cccatttatc tttaatgtat cagatgcttg tattaccgta
    14941 ggagtgatat taattctgct ttttcagaaa cgatttttgc aggaaaacaa tacacaaagc
    15001 aattaaaaac tccctagaat tgtaatgggc agccatatgt aaatatacta ctttctcaat
    15061 aatcgcccag tgtttctttc aattgggcaa gggctttgtc tttttgttcg tcgttgggtg
    15121 cactgccatg ccacttgtgg gtgcccatca taaaatctac tccgtagccc atttgtgtgc
    15181 gcattaaaat agcaacaggt ttgccatttt tagatgcagc tttagcttgt ttcaacactc
    15241 gaacaacatc ttccatatca ttacctttca tatcaaatgt ctcccacccg aaagactgaa
    15301 acttagtatg taaatcacct aaatccaata cttgttgcgt gctgccatct atttgtgcat
    15361 cattccagtc aacggttaca ataagattat caactttctt agccgcagca aacatcaatg
    15421 cttcccaaat ttgcccttct tgcaactctc cgtcacctgt taaacaaaaa acaagcttat
    15481 cgtcgttgtt gatttttttg ctcaaggctg ctcctattgc tactgatatt ccttgcccta
    15541 acgaaccact ggcaatacgt atacccggca agccttcatg cgtggtagga tggccttgca
    15601 accgtgagtt gagatggcgg aaagtcccta attctttcac atcaaaatat cctctccttg
    15661 ccaataagct gtaaaacaaa ggcgaaatat gtccgtttga caggaaaaac aaatcctcat
    15721 tttttccatc catactaaat ggaattttaa tatccattat ctcgaaataa agggcagtaa
    15781 gaaaatcggc acagcctaac gagccacccg ggtgaccgct tttgcattga tgtaccatac
    15841 ggacaatatc tcggcgtact tgtgtggcta tattctgaag ttctgaagtt tgcataatga
    15901 aaaatctaaa atttgagtgt gagcaaatgt atgaaattaa accgcatcat cttggccatg
    15961 ggaatttatt ttttacttta tctattacat tttccttcac attctctttt aaattttgag
    16021 ccggattact gttagtactt gtatcttttt gtccagttaa tatctcattg agcttgtttt
    16081 tagcttgttc tttagccgtt tgttttaatg tatccaaagt ctgcttggct tgtgttttta
    16141 cttcttcttt tatctgttgc acttgttgct gcacttgctc ttttaccaca tctttcatgg
    16201 tgcctccgcc aactttccca tccggttttc ccatcgtaat tttaggatcc ttgagtgtac
    16261 cccctacttt taaattcaga cgaataattt ccggcaccat gccgttcaat tgcggtatag
    16321 gattttttgc cagcaagccg gtcgcaaaag agctggcttt gcctaattct ttccacggca
    16381 catcaatggc tacagcataa tctactgttt gatccatgcc ttgtgaacca ccaaccgtca
    16441 tagacaaatt gtttgccttc atggtaaaag gatcaaccat cattcttccg ttcacaaaag
    16501 tagttttgat atttacattc tgaaactgca gtttgtctaa ttgttgtaat tgcgtcattt
    16561 cttttatctt attcaatacc ggaacatttt gtgctattgc taacggaatg ttgacgcctg
    16621 ccgtaccgtt tatcgttttc aagtcgggag ataagtccgg atttaaggtg gtattgatat
    16681 ccatattaga actaaacgta ccggaaatat attgcataat aggagttata tttttggcta
    16741 cccccgagta tttatacaat tgctgaatat caaatgatgt gatgtgatag ttgatttttg
    16801 ctgtgggaat gtctgttttt gtattgtaaa agccacttac aatggcagaa ccacccaaca
    16861 gattcgcact caaattattt agatttaatt gttcgtcttt gagtgtgata tttcccacca
    16921 tatctttaat attcaattta tcatagagca attctttcac actcgccgtt ccgttcaaat
    16981 ctatattatc agggatacgc attacttctt gcgctttttg tgatttggtt ttgttggcag
    17041 aagatgaatc gggcaaaaat tcattggcat ttattttatt agaaacgata ttgatattgc
    17101 cgccgagctt tgctcctttc atcaatacat aaccaattac attgtctaat tttcctttga
    17161 tatcaaaatc gctgcttccg attttagcta tgcactgatc tacggctaca tattgtggtg
    17221 taaaattcaa taacaagttg ctcaccgaaa tgggcttagg tacgtcttta gatcggtatt
    17281 caataccggt agcagcgata ttgccggatg ccaagatatt ttgatagttt ttagctacga
    17341 tatctgattg acgggctcgc acttttaaat ctaaattggc atttcccttc agtgttttta
    17401 tatcatctga aggataaaat ttttgaacat ctgctaaatt caatttgcct tttgccgcca
    17461 agtctatatc aggatttgaa ataggcgtag caatattcag cgaaccgtct atcggttcat
    17521 tggcaatatt aatatgaaag tcagggatgt tgactactgt attatccaga gtaccccccg
    17581 gattcttaac atgggcatca atggcaatat ccttaacagc agtaggcaaa tcgggatact
    17641 gaaaaaagcc gtcttttaca ttgagattaa gtgcgaactg tggatagctc ttgccttgca
    17701 taatgccttt ggcaattcca tcaagttgta attttccgct tgtttttatt ttgttgaagt
    17761 ctttggcata aatggcagga atgagtgaca aaattgattt aaaatctgtt ttatctgctt
    17821 taaattcaat gtccatattg gttgcgctgt tggcttgttg ctgcacaaat cctgtaaaaa
    17881 gcaacccaag atcattgagt gatagtttat tgtctttgaa actgtatttt tttgcaggtt
    17941 ggtcaatatt taatttgctg tccaatttta ttttagcttc tttcaaatat gttatcaaac
    18001 cttgggcaaa ataggctttg gctatttcag ttgtagaggt gtagtcgagt atctctttgg
    18061 tgaaatctcc tttgccgtca tggtttaaat tttggatgtt taatttattg ccggttgtaa
    18121 aatcgttata actcaaattg atattatcta atgtgatttt cttgagtgcc aatgaaaaag
    18181 gtgatgattt tgatgtgtcg gtttcagatg aagatttgat gatattccag ttggtcattg
    18241 aatctttatt tacaatagca ttcagtatta catctttcag ggtaattgcc tcaattttgt
    18301 aattacttcc tttaattaca ctcatcaaat ccagtgatat tttggcatca ccaagatgca
    18361 acaatgtatc tccttcaaaa ggtgctttat ttataatatc tacatccgtt agtgtcaaag
    18421 cgatattggg gaagttttgt atatttttca ggattgataa atcaactttt cctacgtgag
    18481 gcttggcatc aatataattt tcagcttgct gcgttacaat tccgattagt ttatctttaa
    18541 agaaaaacgg gataatgaat gccgcaacaa taataactaa gaaagtcagc aaaagacctt
    18601 taaaaaactt tttcatataa aattacagat ttaaacaagc tacaagatat aaaatgatac
    18661 tgaaaattgc atgaacgaga ttaaaagtgc tgagtaagtg catgaaaaat aaaagtcccg
    18721 ccggaaaagc gggacaaact aactaaacta caaaagaaga aaatttaata tttgtagcgg
    18781 taaaacaagg tatcctcacc gcttcttcta atgtttcttt tcacaatccg gttctggtca
    18841 tcgtagatat agccaaaatc ataagtatct attgtcgtat tggtcatcag attggtctcc
    18901 acacgtttta acaaaacatt tctgttgtca atgccataat ttaatccaca cttccttatt
    18961 acattcaacc cgccgaggta aatgaaatta acacctttgt taaagaatct cgtatctaaa
    19021 tccgggatga ttgtatctga accgtatgtc aattctgctc tgccaaccgg agggcttccg
    19081 tttatactta actcgtattt cagcaaatta ttgttgccgt catactcaaa attaatataa
    19141 tccaatatgg ctactcctga agaatctctg aatgaagtca gcaagtcatt ggtataggta
    19201 aaccgcaatc catttccggc atcatccacc aatttcgtaa tttgcttttt gttgttgaag
    19261 aaaaatgaca cgtttaattc gcctgcacct aaacgatagg ttcgggcata tacataatca
    19321 ctgtgatatt caaattcaac ttttttcaac accacgcctg aatttccggt gtcattcagt
    19381 acaataattt tctgcaaagt accatcacta ttgtaataaa agttggacga ttcaatcaag
    19441 tcaggactgc cggacagcct gccatttaca ttatcctcca caatctgatt ggactgcctt
    19501 atggattgct gcaatggttg cacacggctg tttacatcac tgccacaacg catcaac
//
LOCUS       DAOQII010000011        19181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1314_length_19181_cov_5.796507, whole genome shotgun sequence.
ACCESSION   DAOQII010000011 DAOQII010000000
VERSION     DAOQII010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19181
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1314_length_19181_cov_5.796507"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLF48_01115"
     CDS             complement(<1..723)
                     /locus_tag="PLF48_01115"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNF47949.1"
                     /translation="MTTLQEQIELAWNDRTLLDKNETRDAINKVITLLDKGELRTAEP
                     TAEGWKVNDWVKKAVILYFPIQKMETIEVGPFEFHDKIPLKKNYAELGVRVVPHAIAR
                     KGAFIQKGVIMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQAAPVIIEDDCFIGSRCIVVEGVRVEKEAVLGANVVLTKSTKIIDVSGEKPVEYRGF
                     VPSRSVVIPGTIPKEFPAGTYQV"
     gene            764..1393
                     /locus_tag="PLF48_01120"
     CDS             764..1393
                     /locus_tag="PLF48_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47950.1"
                     /translation="MKRIIVQLNVLLLVLALVMACSRTKEVEEKNNQGAVVKKYSVLK
                     KQPSLKDGKYVGYYDDGKLQEISNYVEGKLNGERRLYWPNGKLMAKENYKMNRMDGYY
                     ISQYESGSNESQGKYKDNLQEGVWAFYYEKPANTLKQKLTFKEGRIEGPASEYYMNGR
                     VESSGNKIELFEDMDVYDGKIEFYDSLGNLIKIAQYEKGKQISKEEIKK"
     gene            1402..1896
                     /locus_tag="PLF48_01125"
     CDS             1402..1896
                     /locus_tag="PLF48_01125"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNF47951.1"
                     /translation="MRKGRIEAFSDGVLAIIVTIMVLELKVPEGAEWKDLSALIPKFL
                     SYVLSFMMVLIYWNNHHHMFQTVKKVDGKILLANGALLFFLSLLPFATAWMGETHFRS
                     TPVALMGIVLLLSGTAYLLLVKTIVDSHGQDSILANAIGTDYKGNISWIGYLVGIFLS
                     RSYP"
     gene            complement(1992..3017)
                     /locus_tag="PLF48_01130"
     CDS             complement(1992..3017)
                     /locus_tag="PLF48_01130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNF47952.1"
                     /translation="MDIFQQTETLLQEIHDTTISSADEVEKFRIRFLGTKNILKDIFS
                     EIKNVPNERKKEFGQLVNSVKQAAEEKFQAAQELFMQTNSINKTAIDLTLPGDNLALG
                     ARHPIQIVKNKIVEIFNRLGFVVEEDREIEDDWHNFTALNMPEDHPARDMQDTFFINL
                     NPDIVLRTHTSSVQVRVMDRQKPPIRILSPGRVYRNETISARAHCYFHQVEGLYIDEN
                     VSFADLLQTLDYFVKELFGQETKIKLRPSYFPFTEPSAEVDVTCFICGGKGCNVCKYT
                     GWVEILGCGMVDPKVLENCNIDAEKYSGFAFGMGVERITMLKYQIKDIRLLSENDIRF
                     LEQFRTA"
     gene            complement(3082..4158)
                     /locus_tag="PLF48_01135"
     CDS             complement(3082..4158)
                     /locus_tag="PLF48_01135"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNF47953.1"
                     /translation="MRKVYIFLLILAILPRCKKETYTSSANKPLTTIAFGSCCAQYLE
                     DKSIFKNIIAQHPQLYIAGGDNVYADFFALAPGTPQYIEGAYKQLFASGYWYEFFNTV
                     PVLPIWDDHDTGENDGTSNNPVKGFAKEMFFKYWKIPQEAPRHNRPDGGIYDVQYYGD
                     DDHKVQIILLDLRWNHTPYKTSGAGAALSGYDTMLSPDATILGETQWQWLEQELLKPA
                     KVRIIMSSLQFNSLYSGGENWAILPNEKQKMYDLIKSTQANGVVFLSGDVHFAELTKT
                     QPAGMYPIYDVTASGLTHCERKVATTNDAIRVGNGYGYVNYGIIRIDWDKTPVELKLE
                     VYGKTNPQDQPVISHAVTLDELKF"
     gene            4260..4898
                     /locus_tag="PLF48_01140"
     CDS             4260..4898
                     /locus_tag="PLF48_01140"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNF47954.1"
                     /translation="MFNLFVSALKMQFSQQLPGKPAQQLLKPYLKISKALDAPVPYNA
                     KKSAVMALLFPVDDQPHLLLIERNEYKGAHSGQLAFPGGKIEKGDTSYLATALRETHE
                     EIGVSPEQIEVLGGLTELYVLASNFLVYPYVGTLAKKPDFNIDEREVKQILEIPVAYF
                     LSAKIEEKQIKSAIGIKLNAPYYDINGKTLWGATAMMISELMWMVKASGYFK"
     gene            complement(4900..5244)
                     /locus_tag="PLF48_01145"
     CDS             complement(4900..5244)
                     /locus_tag="PLF48_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF47955.1"
                     /translation="MQHIGDKVKKIRELKNFTQDYVAKQLNMSLANYSKIERDEIQLT
                     IDRLEKFATIFQLKSYLDILTFDEKFFFNIHSNQNGNGYINNYYASNEAIASRIAQLE
                     ERVQNLENKKQK"
     gene            5393..6943
                     /locus_tag="PLF48_01150"
     CDS             5393..6943
                     /locus_tag="PLF48_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF47956.1"
                     /translation="MKKLIVFLFLLISTGMFMACKKEQLKDNPQQLNKLVEDSTSSNS
                     SQSLRVAPPGSGTIPFATMSLLESPVRDYEFFGMNPPSSLSYLCGTMDYHCCNDICLG
                     TPTGTLVEASSKSACQNVCIIPGDGFGRPVAYIGSYGTDPRQKYYVYLPDNVNANSPI
                     VIFIHGGAWMLGPDPATTNGPVITFSDDPSSATLLNDLLSSPNDFVVVSLLYRLVKYG
                     DNNAEIDANTIGWVDQVNDIDAAILHMRANFGTCLYGHPLNANNIQLIGESAGGHLAL
                     MWAYTKANTSYIKSVISCYAPTNVTQFAAKLNNKPYTYICGTDYFYNDINGTYPTHFP
                     YYFATDITTPNQITSSISPLTCTINTIFWGAKNILTGKPFYSTPNPTPGLRVVDLYKE
                     AESAAKNRTNPVPIFTQYSPVNALNTSRIIPTFIMHGTDDWIAPYKYCTDGMKNKMIS
                     TGGLIDSLNSTYTGHKDVPTTYTTSNKHLLKTYTNANHGFASGTSANGADYTTIRADI
                     LKWFNGHK"
     gene            6951..7793
                     /locus_tag="PLF48_01155"
     CDS             6951..7793
                     /locus_tag="PLF48_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47957.1"
                     /translation="MRFIFIFTITFLLIGCCKESMPNQNNMLSGKLHYTYYGNDTIPF
                     YLFIYDTITGNLKEIRHDTEVIIAVKDIGKYTMVMSGYNSGTCLYTCNDKRQITSIRG
                     VDANLQPNGADYIPDPVLRNDSINIIFQNNSYNYPSPSYRNIQCYDFSYLDGNCVSSK
                     INYETTGFFSTNYYSYSDTTHYEFTNFMMKNFMDQFPFTWLFTNISLPHQGLELVAAA
                     INGFYITPLNKNLMSSSEEAHAGAINKLYYAYEFDSRNNLIKTIVSNQTHTDSSITRY
                     AYYQ"
     gene            complement(7869..8543)
                     /locus_tag="PLF48_01160"
     CDS             complement(7869..8543)
                     /locus_tag="PLF48_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47958.1"
                     /translation="MKKFLLSCLIVAGTFALKAQSNIAVNEIRQYMSKGEQNGFEVVL
                     NGSKPDDVKDALSKWTKKNKGKYVTEKKNPEIFLDNVTLPLVSANTVDIYVTITPLDK
                     GSKMDMFVDLGGAFISSSAYGQQYTAMEAAIKKFANDLAIDATKDQLKSEEKVLKDLN
                     GDLKDLTKKKEDYIKDIEKAKKEIQEREADIQKNDADQAAKQQQISIQQQIIETIKTK
                     LNSMKF"
     gene            complement(8553..10754)
                     /locus_tag="PLF48_01165"
     CDS             complement(8553..10754)
                     /locus_tag="PLF48_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47959.1"
                     /translation="MKKTLFLILLIIPFLGMSKKKWDAVYINKLIATGEIDKVIDFYK
                     ARYFGDSRDPQDAFRIADLYVKKKDYKSALEWYEKEKQLLKSNKTNFLNYANTYRLNG
                     NYKEALDAYTMYAAETGDASKVMDYAYLCDKLIRASAMQNLYRVENYPYNTSADETNV
                     TTLRNNLIYTQESGDGKDKSYQLKQAIREYDYFLEPVAVITKEIPYKNIDKVSYTKDG
                     NTVAFSVSDRNEKNKLNPVVQQIYVAENLGGNFLNIKPFPFNVPGYNLTDPAFNSDGT
                     VLYFSSNQTGGYGGYDIWKSVLQNGNWTKPVNLGKLINSKFNENAPYLIQDKQENKLY
                     FSSDRENGFGGWDIYTAKMIDDVWQDVQLLPYPINTNKDENSMVYATDTKTGYVSSTR
                     DGGKGGNDIYRILPFHLKMNVHLTDENGNKLDYALVQLSDGNTKLDEGVSNEDGTADF
                     EVAKDFSYAIKATKDGYAPATAKTDTKGFVNGDSVELNLVLKKLAANANSTSTFSSSE
                     YVTFIGRIIDGAANKPATQAKMRMINLNSSRIREVDIDESGVYQLKLFLNNNYKIIIE
                     NGNYKINDELTTYGLEEGQIKTKDYLLNGNKFKLTNNRVFNATTIPAELSALISKENK
                     PSIQKVTPATTVTGTTSSPVSPVLSTTSQNGSFYKINLGSYAQDNVAFDDLAALGSIQ
                     KTNSYNQYVYRLGDFSTLDDAKLALEFAQNKGYFVAFILQYDNGKITNIIK"
     gene            complement(10771..13569)
                     /gene="polA"
                     /locus_tag="PLF48_01170"
     CDS             complement(10771..13569)
                     /gene="polA"
                     /locus_tag="PLF48_01170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNF47960.1"
                     /translation="MKKLYLFDSFALIFRAYFAMQKNPLINSKGMNVSAISGFLNTIW
                     EIKTKYQPTHYAIVFDAPAQTDRQAEYDFYKANRQETPDDIVTAIPYIKEIIKAMNMP
                     VVELAGYEADDLIGTLAKKAAKDDFECYIVTFDKDLGQVCEEKIFIHRPPFMGRPAEV
                     LTVDILKEKWGIERPEQIIDILGLWGDAVDNIPGVKGIGEKGAKELIKEFGSIDNIYE
                     NLDKVKGKMKDKLLEHKEMAFISKQLATIIIDAPIDWDAEEYKLKPFNKEKLSEIFAD
                     LEFRTLGKRILGDEYSLNEIKAQTQQKQAAFAQTSLFGEPDEKADDDAITEQAKNIHN
                     TPHDYFVADTTDKINNLISLLSKQEKFCFDTETTSVDANNCELVGMSFSIEPHKAYYV
                     PIAENQQTAQETVNLFKPLFENGQITKIGQNIKFDALVLKWYGVNLQGTLYDTMLAHY
                     LMDADSRHNMDILSENYLGYTPVSITELIGKKGAKQGSMRDVPLEKITEYAGEDADIT
                     FQLYKRFRKDTDQTHLSRLFYEVETPLIPVLTDMEYEGINVDVSFLKQYSDEIKLEIV
                     QLKDDIYALTKAQFNIDSPKQLGEILFDGLKIKYPGKKTKTGQYSTDEETLLKIAEQH
                     AVVDKLLDYRELTKLKSTYVDAIPQLINPKTHRLHTTYNQAIAATGRLSSINPNLQNI
                     PVRTERGRKIRQAFIPKNEDYTILSADYSQIELRIIASVSGDENMISAFKKQLDIHTA
                     TAANVFGVALDEVTPEMRRQAKMVNFGIIYGISAFGLAQRLGIPKTQAGELIENYFAT
                     YPNIKAYMDNTIKFAREHGFVETLLGRRRYIRDINAGNFTVRGFAERNAINAPIQGSA
                     ADMIKIAMINIHKQLAGHKMQSKLLLQVHDELLFDVYKPEKEELQKMVEREMQNALPL
                     LVPVEVSSGFGNNWLEAH"
     gene            complement(13708..13781)
                     /locus_tag="PLF48_01175"
     tRNA            complement(13708..13781)
                     /locus_tag="PLF48_01175"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Ile,seq:cat)
     gene            14001..15428
                     /locus_tag="PLF48_01180"
     CDS             14001..15428
                     /locus_tag="PLF48_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF47961.1"
                     /translation="MATIHAQDTISIACYNLLNYPGSTDLLRNPGFRTVISALHPDIL
                     VCEEVNEAVAANEFRDSVMKIVDTLYNMGTWINCQSSSSENENVVYYRKDKFTFLSNT
                     PILTDLRDINRFSFVHINTGIPFSVFGVHLKAGNTATDNSRKALEIDSLRKYTNTLPF
                     GSNLVVCGDFNFYTSSESGYTKLLSDNITYDGEFYDPVTTMTGVWNNPAYAIYHTQST
                     RKSEVFFGGATSGLDDRFDLIMHSQAVKDPGGMDYIPGSLKAFGNDGNHFNDSIKAQP
                     NTAVTPAVADALYYVSDHLPVIEKFKFSPVILPVELSSFYALCKDRLTTLVWTTESET
                     NTGQFVLEQSDGNANWTAIDSFPAAYMSNLHKIYQTELNETSGGINYYRLKIADTDNS
                     YVYSGVIATNCTESATPYLHVYPNPFKNNLQLFTNIAESELNIRDMLGRTYNYSATLI
                     QTLPSGVYILFSSNHSVSPVKIIKE"
     gene            complement(15441..15686)
                     /locus_tag="PLF48_01185"
     CDS             complement(15441..15686)
                     /locus_tag="PLF48_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47962.1"
                     /translation="MSRNKKTIKGKVEVKKFGSGTKSEHNAIYIRDNLTNEEYVLRLK
                     GTNPFENNELKSFIGKEVKAKGILDDYLFLADKIAEV"
     gene            complement(15673..17217)
                     /locus_tag="PLF48_01190"
     CDS             complement(15673..17217)
                     /locus_tag="PLF48_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNF47963.1"
                     /translation="MKYDKVILTNKTALDLKYKTDSKFIIAALNKLVQADATKNLKSK
                     LILLDDKTTMNAINVNRVRQFNDAEQVKIAIDGIYSKLSPDYILLVGGTEIIPHVRLT
                     NPCYDPNDDIDRFIYSDLPYACNTAYSTSASAFTSPVRVVGRIPDITGIKNPDNLIKL
                     IQNSINNIPRKKTVYDSIFGLSAKIWEKSTNLSLSNITGSPTPAKTCPPFNHTTASHG
                     DAELGSMMHFINCHGAGNTPEFYGQRDRTDGSFPVSFITTDVVNKITYAAMVAVECCY
                     GAQLYYASTANEIPICNNYLLHGALAYVGSTNIAYGPAVGNGLADLITQYFLRKAKEG
                     ASIGRAFLEARLDFLEDAQPDLDAEELKTLAQFVLYGDPSVCLVEEQVSPDSDSFAPK
                     TQKTIEQNQRKNRRYRLMEKGLQLGISIGIPKKTNKPKSASASALLRTAINTALKENN
                     FEKSNAKVYIIKKPTHSAGKSASRKVEFHTYSKTKKVGKVINIEKLVLKVVNSDIVGI
                     RKYVSK"
     gene            17723..18703
                     /locus_tag="PLF48_01195"
     CDS             17723..18703
                     /locus_tag="PLF48_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNF47964.1"
                     /translation="MEESIATERILQESDNRFVLFPIKYAQIWEMYKKHVQAFWVAEE
                     IDLSHDLIHWEKLTEDEQHFIKHVLAFFAASDGIVNENLVVNFMQDVTIPEARCFYGF
                     QIAMENIHSETYSLLIDTYIKDTAEKNKLFHAIETLDCVKLKAKWALKWIESAPSFAH
                     RLVAFCAVEGIFFSGSFCSIFWLRERGLMPGLAHSNELISRDEGLHCQFACLLYSMLE
                     NKLSKEDIYAIICDAVEAEKVFVRDSLPVALIGMNSDLMCQYIEFVADSWIVALGYPK
                     VYHATNPFPFMENISLEGKTNFFERRVSEYKKAGVGVAKEDQTFGIVEDF"
     gene            18740..>19181
                     /locus_tag="PLF48_01200"
     CDS             18740..>19181
                     /locus_tag="PLF48_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNF47965.1"
                     /translation="MYVIKRDGRKEPVHFDKITTRISRLSYGLSNRFVVPHKVAQNVI
                     QGLYDGVTTTELDELAARTSASMTVVHPDYAVLAARIAVSNLHKNTKKTFSRIIEELY
                     NYIDPKTNKKAPLIADDVYEIIMANRDRLDASIIHSRDYNYDYFG"
BASE COUNT         5668 a         3729 c         3437 g         6347 t
ORIGIN      
        1 tacttgatac gttccggcag gaaattcttt tgggatagtg cccggtatca ccacacttcg
       61 agatggaaca aaacccctgt attctacagg cttttcacca cttacatcta taattttagt
      121 tgatttagtc aagacaacat tagcacctag tacggcttct ttttctacac gcacgccttc
      181 taccacgata catcttgacc ctataaaaca atcatcttcg ataataacag gtgctgcctg
      241 tacaggttct aatactccac cgatgcctac gcctccgctt aaatgtacat ttttgcctat
      301 ttgcgcacaa ctgcccacgg tagcccaggt atctaccatc gtgccactat ctacatacgc
      361 cccgatattg acataagacg gcatcataat cacgcctttc tgtataaaag cacctttgcg
      421 ggcaatggca tgaggtacta cacgtacacc caattcagcg taattttttt tcaatggtat
      481 cttgtcgtga aattcaaaag gacctacttc tattgtttcc attttttgta tagggaagta
      541 gagtattact gctttcttca cccagtcgtt cactttccat ccttctgcag ttggttctgc
      601 cgtgcgtaat tcgcccttgt ctaagagcgt tattaccttg ttgatagcat ctcttgtttc
      661 atttttatcc aataaagtcc ggtcgttcca ggcaagttct atctgttctt gtaaagttgt
      721 catatttttt agaaatttga atcaaaaata aacataaatc aaaatgaaaa gaattatcgt
      781 tcaattaaat gtgctgttgt tagtacttgc acttgtaatg gcttgtagcc gtacaaaaga
      841 agtagaagaa aaaaacaatc agggggcagt tgttaaaaaa tattccgttt taaaaaaaca
      901 accatcgtta aaagatggta aatatgtagg ttattatgac gatggcaaac tacaagaaat
      961 ttccaattat gttgaaggta aattaaatgg agaacgcagg ctatattggc ctaatggcaa
     1021 gctgatggca aaagaaaatt acaaaatgaa ccgcatggat ggatactata tttcacagta
     1081 tgaaagcggc agcaacgagt cgcaaggaaa atataaggat aatctgcaag aaggtgtatg
     1141 ggcattttac tatgaaaaac ctgccaacac acttaaacaa aaattgacat ttaaagaagg
     1201 ccgaattgaa ggacctgcct ctgaatatta tatgaacggc agagtagaat ccagtggaaa
     1261 taagatagag ctttttgaag atatggacgt ttacgatggc aagattgagt tttatgattc
     1321 tttgggaaat ttgataaaaa ttgcccaata tgaaaaaggc aaacagattt cgaaagaaga
     1381 aataaagaaa taaagaaatc tatgagaaaa ggcagaatag aagcattcag cgatggcgta
     1441 ttggcaatta tcgttaccat catggtatta gagttaaaag taccggaagg tgcagaatgg
     1501 aaagatttat cggcattaat ccctaaattt ttaagttatg ttttgagctt tatgatggta
     1561 ttgatatact ggaacaatca ccaccatatg tttcaaactg taaagaaagt agacggaaaa
     1621 atattacttg caaatggcgc attattattt ttcttgtcat tattgccgtt tgctacggca
     1681 tggatgggcg aaactcattt ccggtcgaca cctgttgcct taatgggaat tgtactttta
     1741 ctttcaggaa ctgcgtattt gctattagta aaaactatag tagattcaca tgggcaagat
     1801 tcaattttag caaatgctat cggtacggat tacaagggca atatttcctg gattggctat
     1861 ctcgtgggta tatttttatc tcgtagctat ccataagtat ccgcagccat ttatttagta
     1921 atcgcactta tgtggtttat tccggataaa cgtatagaac gactaatgca cgatgaaacg
     1981 actgaatagg cttaagccgt acggaactgc tctaaaaaac gtatatcatt ttcagacaat
     2041 aagcgtatat ctttaatctg atatttgagc atggtaatgc gttcaactcc cataccaaag
     2101 gcaaatcccg agtatttttc agcatcgata ttgcagtttt cgagaacttt cgggtctacc
     2161 ataccacagc ctaagatttc tacccatcct gtatatttac agacattgca tccttttcct
     2221 ccgcaaataa aacaagttac atccacttcg gcacttggct ccgtgaatgg gaaataggaa
     2281 ggacgtaatt ttatttttgt ttcttgcccg aaaagttctt tcacgaaata atccagtgtt
     2341 tgcaataaat cggcaaatga cacattttca tcgatataca agccctcaac ctgatgaaaa
     2401 taacaatgtg ctctagctga tatcgtttcg tttcgataca ctcgcccggg agataaaatg
     2461 cgtattggcg gtttttgcct gtccattact ctcacttgaa cagaagaagt atgcgtgcgc
     2521 aatacgatat ccggattcag attaataaag aaagtatcct gcatgtctct tgccggatgg
     2581 tcttcgggca tatttaatgc ggtgaaatta tgccagtcat cttcaatttc acggtcttct
     2641 tcaacaacaa aaccaagccg attgaagatt tcaacaatct tgttttttac aatctggata
     2701 ggatgtcgtg cccctaatgc cagattatct ccgggcaaag tcaaatcaat agccgtctta
     2761 tttatgctgt ttgtttgcat gaataattct tgagcagcct gaaacttttc ttcagcggct
     2821 tgctttactg aattaactaa ttggccaaat tcttttttgc gttcattagg aacatttttt
     2881 atttctgaaa aaatgtcttt caatatattt ttagtaccca aaaagcgtat tctgaatttc
     2941 tctacttcat cagccgatga aatcgtagta tcatgaattt cctgtaataa agtttctgtc
     3001 tgttgaaaaa tatccattat caaaaataaa aaaaagaggc acacaatatg cctcttttct
     3061 attcaaggaa ttattaaatc gtcagaattt taattcatct aaggtaacag catgtgatat
     3121 aaccggttga tcttgcggat ttgtttttcc ataaacctct aattttaatt ctaccggtgt
     3181 tttatcccaa tcaattcgta taatgccgta atttacatat ccataaccat ttcctacacg
     3241 aatggcatca ttcgtagtgg ctactttgcg ctcacaatgt gttaatccgc tggccgtaac
     3301 atcataaatc ggatacatac cggcaggttg ggttttggtt aattcagcaa agtgtacatc
     3361 cccactcaaa aataccactc cattcgcttg tgtagactta atcagatcat acattttttg
     3421 tttttcgttg ggtaaaatag cccagttttc accaccactg tataatgaat taaattgtaa
     3481 gctggacata atgatgcgta ctttagccgg ttttaacagt tcttgttcca accattgcca
     3541 ttgtgtttcg ccgagaatcg tagcatctgg gctaagcata gtatcgtatc cactaagcgc
     3601 agcacctgct ccggatgttt tgtatggagt atgattccat ctcaaatcca ataaaataat
     3661 ctgtacctta tgatcgtcgt caccataata ttgcacgtca taaattcctc catccgggcg
     3721 attatgacga ggagcttctt gtggaatctt ccagtattta aaaaacattt ctttagcaaa
     3781 accttttacg ggattatttg aagtaccatc attttctcct gtatcatggt catcccagat
     3841 tggtaacaca ggtactgtat taaaaaactc gtaccagtat ccgctggcaa aaagttgctt
     3901 ataagcacct tcaatatact ggggagttcc gggagctaat gcaaagaaat ctgcatatac
     3961 attatcaccg cccgctatgt acaattgggg atgctgtgca atgatatttt tgaagatact
     4021 tttatcctca agatattgtg cacaacaaga accaaatgcg atggtggtga gtggcttatt
     4081 tgcggatgaa gtataggttt cttttttaca tctgggcagt attgccaaga ttaacagaaa
     4141 aatatagact tttctcatat cgtaaattat tatgaatttg atttaaacca aaagtatgat
     4201 ttttatttga caaactcaaa atattgttga attaatttta ttccacttac attcgcaaga
     4261 tgtttaatct gtttgtttcg gcattaaaaa tgcaattttc acaacagctc ccgggcaagc
     4321 ctgcacaaca acttctaaaa ccttatctca aaatttcaaa agcactggat gcccctgtac
     4381 cgtataatgc aaaaaaaagt gccgtgatgg cattgctgtt tcccgttgat gaccaacccc
     4441 atcttttgct gattgaacgc aatgaataca agggtgcgca tagcgggcag cttgcttttc
     4501 ccggtggtaa gattgaaaaa ggagatacct cctatttggc aacagccttg agagaaaccc
     4561 atgaggagat tggcgtatca cctgaacaga ttgaagtgct tggcggattg acagagctat
     4621 atgttttagc cagcaatttt ttagtttatc cttatgttgg tactttagcg aagaagcctg
     4681 actttaacat tgatgaacga gaggtaaaac aaattttaga aattccggta gcttattttt
     4741 tatctgctaa aattgaagaa aaacaaataa aaagtgctat cggtattaaa ctcaacgcac
     4801 cttattatga catcaacggt aaaacattgt ggggtgcaac agccatgatg attagtgagc
     4861 tgatgtggat ggtaaaggca agtggctatt tcaaataatt tatttctgtt ttttattttc
     4921 cagattttgg acacgttcct ctaattgtgc tatacgagaa gctatcgctt catttgacgc
     4981 atagtaatta tttatatacc cattaccgtt ttgattactg tgtatattaa agaaaaattt
     5041 ctcatcaaat gtcagtatat ctaaatacga ttttagctga aatatagtag caaacttttc
     5101 taaacggtct atcgtgagct gaatttcatc acgctctatc ttagaataat ttgccaatga
     5161 catattcaat tgtttggcta catagtcttg tgtgaaattt ttcaattctc gtattttctt
     5221 cactttatct cctatatgtt gcatggtaga atattatagc gtaaaagtaa aatatttcta
     5281 ctaataaata aactcaaact tcaaaaaagt atatttattt tacagtatct aatatttttt
     5341 aacttaaatt ttagacaaaa aaatattttt taacctttaa cccccttgca tcatgaagaa
     5401 attaatcgta tttttattct tgttgatatc aacaggtatg tttatggctt gtaaaaaaga
     5461 gcaattaaaa gacaatcccc aacaactaaa caaattggtt gaagacagta cttcaagcaa
     5521 tagtagccaa tcactaagag ttgctccgcc tggaagtggc actattcctt ttgccaccat
     5581 gtctttatta gaatccccgg taagagatta tgaatttttt ggaatgaatc ctccctcatc
     5641 cttaagttat ctttgtggta ctatggatta tcattgttgc aatgatatct gcttgggaac
     5701 accaaccggc acattagtag aagctagcag taaatcagct tgtcagaacg tatgtattat
     5761 acccggagat ggctttggca ggcctgttgc atatattggc agttatggca ctgacccccg
     5821 tcagaaatat tatgtgtacc tacccgataa tgttaatgca aattcgccaa ttgttatatt
     5881 tatacatggt ggagcttgga tgctgggtcc cgatcctgcc actactaacg gtccggttat
     5941 tacattttca gacgatccat ctagtgcaac cttactaaac gatttactat catcacccaa
     6001 tgactttgtt gtagtatctc ttctctaccg tttagtaaaa tacggtgata ataatgctga
     6061 aatagatgcg aatactattg gttgggtaga tcaagtgaat gatatagatg ctgctatttt
     6121 gcacatgaga gccaattttg gcacctgctt atatgggcat ccattgaatg ccaacaacat
     6181 acagttgata ggtgaaagtg ccggagggca tttggcattg atgtgggcat atactaaagc
     6241 taatacctcg tatataaaat ctgttatttc ttgctatgca cctacaaatg taacccagtt
     6301 tgctgccaaa ttgaataata aaccttatac ctacatatgc ggaacagatt atttttacaa
     6361 tgatataaat ggtacttacc ccacccattt cccttactat tttgcaacag acattactac
     6421 acctaatcaa attacttctt ccattagccc gcttacatgt acaattaata cgattttttg
     6481 gggagcaaaa aatattctta ctggcaaacc cttttatagt acaccaaatc ctacacccgg
     6541 attaagagta gtcgatttgt ataaagaagc cgaaagtgca gcaaagaacc gcaccaaccc
     6601 cgtgccaata tttactcaat atagtcctgt aaatgcttta aatacaagcc gtattatacc
     6661 cacctttatc atgcacggta ctgatgactg gatagccccg tacaaatatt gtacagacgg
     6721 catgaaaaat aaaatgatta gtaccggagg attgattgat tcattaaaca gcacctatac
     6781 agggcataaa gatgtaccga ctacttatac tacatcaaac aagcacttat taaaaaccta
     6841 cacgaacgca aatcatggtt ttgcgagtgg tacaagtgca aatggagctg attatacaac
     6901 catacgcgca gatattttaa aatggtttaa cggacataaa tgagctaaac atgagattca
     6961 tatttatatt taccattact tttctattaa tagggtgttg caaagaaagt atgcctaacc
     7021 aaaataatat gctttcgggc aaattgcact atacttatta tggaaatgac acgatcccct
     7081 tttatttatt tatttacgat actattacag gaaatttaaa agaaattagg catgatactg
     7141 aagtaattat agctgtaaag gatattggaa agtatacaat ggtgatgagc ggatataact
     7201 ccgggacttg tttgtatacc tgcaatgata aaaggcaaat tacttctata cgcggagtag
     7261 atgcaaactt acaaccaaac ggtgccgact atattcctga ccctgtatta agaaatgatt
     7321 caataaatat tatatttcaa aataattcat ataattaccc atcgcccagt tacagaaata
     7381 tccagtgcta tgattttagt tatttagatg gaaattgtgt cagttctaaa atcaactatg
     7441 agactacagg attctttagt acaaattatt atagctactc agatacaaca cattatgaat
     7501 ttacaaactt tatgatgaaa aattttatgg atcaatttcc ttttacttgg ttatttacta
     7561 atatatcctt accacatcag ggattagagc ttgttgctgc agctataaac ggtttctata
     7621 ttacaccatt aaataagaat ttaatgagta gttcagaaga agcacatgct ggtgcaatta
     7681 acaaacttta ctatgcttat gaatttgact ctcgtaataa tttaattaaa actattgtta
     7741 gtaatcaaac tcatactgat agttctataa ctagatatgc ctattatcaa taagtcagga
     7801 gggcggcgct atgcgccgcc ctcctgtttc tatataatat caacaatgtt tcttgttgat
     7861 tacctaactt aaaacttcat ggaattcagc tttgttttta tcgtttcgat aatttgttgc
     7921 tgaatagaaa tttgttgttg tttagcagct tggtctgcat cattcttttg gatatctgct
     7981 tcacgctctt gtatttcttt ctttgctttt tcgatgtctt taatataatc ttctttcttt
     8041 ttagttaaat cctttaagtc gccgttcaag tctttcaata ctttctcttc agatttcaac
     8101 tggtctttgg tagcatcaat ggctaaatca ttcgcgaatt ttttaatggc agcttccatg
     8161 gcagtatatt gctgaccata agcagaagat gagataaatg cgccacctaa atccacaaac
     8221 atatccattt ttgaaccttt gtccaatggt gtgatggtta cataaatatc tacggtattg
     8281 gcgcttacca aaggcaaggt tacattatcc agaaagattt cagggttttt cttttccgtt
     8341 acatacttcc ctttattttt tttcgtccat ttcgataagg catcttttac atcatcgggt
     8401 tttgagccgt tcaatactac ttcaaagcca ttttgttcgc ctttgctcat atactggcga
     8461 atttcgttta cggcaatatt agactgcgcc ttcagggcaa aagttcctgc cacaatcaga
     8521 caagacaata aaaatttttt catgagatta aattatttaa taatattagt gatttttcca
     8581 ttatcatact gcaaaatgaa tgccacaaaa tatcctttgt tctgagcaaa ctccaaagcc
     8641 agttttgcat catccagtgt actgaaatca ccgagtcggt aaacatactg attataagaa
     8701 tttgttttct gtatcgaacc caacgctgcc aaatcgtcaa aagctacatt atcttgtgca
     8761 tagcttccca gatttatctt gtaaaaacta ccattttgcg aagttgtaga cagaacagga
     8821 cttaccgggc tacttgtcgt gccggtaacg gttgtagcgg gagttacctt ttgaattgac
     8881 ggtttatttt ctttactaat caatgcactt aattcagcag gaattgttgt tgcgttaaac
     8941 acgcgattat tggtcagctt gaatttattc ccatttaaca aatagtcttt tgttttaatc
     9001 tgtccctctt ccagcccata ggtcgtcagt tcgtcattga ttttataatt tccgttttca
     9061 atgataattt tataattgtt gtttaaaaat aattttagtt gataaacacc actttcgtca
     9121 atatcaactt cccgaatacg gcttgagttc aagttgatca tacgcatttt tgcttgtgtg
     9181 gcgggtttat tggcagcacc gtcaataatt cttccaataa aagtaacata ctccgatgaa
     9241 gaaaaagtgg aggtactgtt ggcatttgcc gctaactttt tcaataccag attaagctca
     9301 actgaatcgc cgtttacaaa tccttttgta tccgtttttg cagtagccgg tgcatatccg
     9361 tctttagttg ctttaatggc atatgaaaaa tcttttgcca cttcaaaatc agctgttccg
     9421 tcttcgttgc tcactccttc atctaatttg gtattcccgt ctgataattg caccagtgca
     9481 taatctaatt tatttccgtt ttcgtctgta aggtggacat tcattttcag atgaaaaggc
     9541 aatatgcgat aaatatcatt tccgcctttg ccgccatcac gtgtagaaga tacatatccc
     9601 gttttggtat ctgtggcata taccatgctg ttttcatctt tattggtatt tattggataa
     9661 ggcaataatt gtacatcctg ccaaacatca tctatcattt tagccgtata aatatcccaa
     9721 ccgccaaaac cattttcacg gtcggatgaa aagtaaagct tattttcctg tttatcctga
     9781 atgaggtagg gtgcattttc gttgaattta gagttaatca atttgcctaa attcacaggc
     9841 tttgtccagt tgccattttg caatacagat ttccagatat catatccgcc atagccacct
     9901 gtctggtttg aagaaaaata gagtacggta ccatcgctgt tgaatgcagg gtctgtgaga
     9961 ttatatcccg gcacattaaa aggaaatggt tttatattca ggaaattacc acccagattt
    10021 tcagccacat aaatttgctg tacaaccggg tttaatttat ttttttcatt tcggtctgaa
    10081 acggaaaatg cgaccgtatt cccgtctttg gtatagctta ctttatcgat gtttttatat
    10141 ggaatttctt tagtaataac agctaccggc tccagaaaat aatcgtactc cctaatggct
    10201 tgcttaagct gataagattt atctttcccg tcacctgatt cctgcgtgta aataagatta
    10261 ttgcgcaagg tagttacatt ggtttcgtca gcagatgtat tgtaagggta gttttctact
    10321 ctgtataagt tttgcatggc agaagccctt atcagcttat cacataaata agcataatcc
    10381 attactttgg atgcatcacc cgtttctgcg gcgtacatgg tataggcatc caatgcttct
    10441 ttataatttc cgttcagcct gtaggtgttg gcataattga gaaaattggt tttgtttgat
    10501 ttcaggagtt gcttttcttt ttcataccat tccagagccg atttataatc tttctttttt
    10561 acatacaaat cggcaatgcg aaacgcatct tgcgggtcgc ggctatcgcc gaaataccgt
    10621 gctttgtaaa aatctattac tttatcaatt tctcccgtcg ctatcagttt attgatataa
    10681 acggcatccc attttttttt ggacatgccc aaaaacggga taatcaacaa aatcaaaaac
    10741 aatgttttct tcatacgctt atttttaggg ttagtgtgct tccagccagt tgtttccaaa
    10801 tcccgaagat acttctacgg gaactaacaa aggtaatgca ttttgcattt ctctttctac
    10861 cattttttgt aattcttcct tttcaggttt atacacatca aacagcaatt cgtcgtgcac
    10921 ctgcaacagt aacttagatt gcattttatg tcctgcaagt tgcttgtgta tgtttatcat
    10981 ggcaattttt atcatatcag cagccgaacc ttgaatgggt gcattaattg catttcgttc
    11041 tgcaaatcct cttacggtaa agtttccggc attgatatcc cggatatagc gccttctgcc
    11101 caataaagtt tcaacaaaac catgctctct ggcaaatttt atggtattgt ccatgtaggc
    11161 tttaatgttg ggataggtgg caaagtaatt ttctatcaat tctcctgctt gtgttttagg
    11221 aatacccaaa cgctgtgcca aaccaaacgc agaaatgccg taaatgatac caaaatttac
    11281 catttttgcc tgtctgcgca tctcaggcgt tacctcatcg agtgccacac caaatacatt
    11341 tgctgcagta gcggtatgta tatccagctg ttttttgaaa gccgaaatca tgttttcatc
    11401 cccggaaaca gatgcgataa ttctcagttc tatttgggaa taatcggcag agagtatcgt
    11461 gtagtcttca tttttgggta taaatgcttg cctgattttc cgcccgcgtt ccgtgcgcac
    11521 cgggatattc tgcaagttcg ggttgatgga actcaaccgg cctgttgctg caattgcctg
    11581 attataggta gtgtgcaggc gatgtgtttt aggattgatc agttgcggta tggcatctac
    11641 ataagtggat ttgagtttag tcagctcgcg atagtccaat aacttatcga caacggcatg
    11701 ctgttcggca atcttcagca gcgtttcttc atctgtagaa tattgcccgg ttttcgtttt
    11761 tttacccgga tattttattt ttaatccgtc aaataaaatt tctcccagtt gtttgggtga
    11821 gtcaatattg aattgtgctt tagtcaaagc atagatatca tcttttagct gcacaatttc
    11881 caatttaatt tcatctgaat actgtttcag aaagctgaca tctacattta tgccttcgta
    11941 ttccatatca gtcaataccg gaatcaaagg tgtttctacc tcataaaaca aacgacttaa
    12001 atgggtttgg tcggtatctt ttctaaatct cttataaagc tgaaaagtaa tatcggcatc
    12061 ttctcctgca tattccgtaa ttttctccaa aggaacatca cgcatactgc cttgcttagc
    12121 tcctttttta ccaatcagtt cggtaataga caccggagta taacccagat agttttcact
    12181 cagaatatcc atattatgcc ggctgtcagc atccatgaga tagtgcgcca gcatcgtatc
    12241 gtaaagtgta ccttgcaaat taacaccata ccactttaat actaacgcat caaatttaat
    12301 attttgtcct attttagtaa tttgcccatt ctcaaacaat ggcttgaata agttgactgt
    12361 ttcctgagcc gtttgctgat tttcggcaat cggcacatag taggccttat gcggttcaat
    12421 agaaaatgac atcccgacta actcgcaatt gttggcatcc acagaagtag tttcggtatc
    12481 aaaacaaaat ttttcctgtt tagacaataa gctaatcaga ttattgattt tatcagtcgt
    12541 atcggcaaca aaataatcgt gtggtgtatt gtggatgttt ttagcttgct cagttatcgc
    12601 atcatcgtca gctttttcat cgggctcgcc aaacaggctg gtttgtgcga aagctgcttg
    12661 tttttgttgc gtttgcgctt taatttcatt gagggagtat tcatcgccaa gtatacgctt
    12721 acccagcgtt ctgaattcga gatctgcaaa gatctccgac agtttttctt tattaaaagg
    12781 ttttagtttg tactcttcag catcccaatc tatcggtgca tcaataataa tagtggctaa
    12841 ttgtttagag ataaatgcca tttccttatg ttccagaagc ttatctttca ttttgccttt
    12901 taccttgtcg aggttttcat agatattatc tatagagcca aattctttta tcagctcttt
    12961 tgctcctttt tcgccgattc cttttacacc tggaatatta tccacggcat cgccccataa
    13021 tcctagtata tcaataatct gttcaggacg ttcgataccc catttttctt ttaatatatc
    13081 taccgtgagt acttcggcag gtctccccat aaatggcgga cgatgaataa agattttttc
    13141 ttcgcacacc tgtcccaaat ctttgtcaaa ggtgacgata taacattcaa aatcatcttt
    13201 ggctgctttc tttgccagcg ttccaatcag gtcgtcagct tcataaccag ccagctctac
    13261 cacaggcata ttcatagcct ttataatttc tttgatatag ggaatggctg ttacgatatc
    13321 atccggggtt tcctgacggt tggctttata aaaatcatat tctgcctggc ggtcggtttg
    13381 tgcaggagca tcaaagacga tggcatagtg cgtaggctga tatttggttt ttatttccca
    13441 gatcgtgttt agaaacccgg atatggcaga cacattcatg cctttagagt ttatcaacgg
    13501 gtttttctgc atggcaaaat aggcacgaaa aataagtgca aacgaatcga acaggtatag
    13561 ctttttcata acaaattaaa tgctaaatgt acgaattaat aggaacatgg gtttagaaag
    13621 agcatctccg tatttgcaaa acatttcaat tttttggtaa ataaaaaaag gggttcagct
    13681 atacgctaaa accctcattt tttcttgtgg tcccgcctgg gctcgaacca gggaccccct
    13741 gattatgagt caggtgctct aaccagctga gctacaagac ccttgcaaac tttcgtttac
    13801 aatcgaaccg caaaaataca attttatttt taaatcccga ataattctta aattctctgt
    13861 ctaaaatatt ttctccccta atttgttaaa attcaaggtc aacttactcg ttaatgttgc
    13921 ttaccgtatc tttgtgtggc taaacaacaa ctaatttgag gagattttac acggtaaccc
    13981 tgttcttact gttttcttag gtggctacta ttcacgcgca agataccatc agtattgctt
    14041 gctacaattt attgaattac cccggcagca cggacttatt gcgcaatccc ggcttccgca
    14101 cggtcatttc tgccttgcac cccgatattt tagtgtgcga ggaagtaaat gaagccgtgg
    14161 cagccaatga atttcgcgac agcgtgatga aaattgtaga cacactttat aacatgggca
    14221 catggataaa ttgccaaagc agttcctcag aaaatgaaaa tgtcgtttac tatagaaaag
    14281 ataaattcac atttttatcc aatacgccta ttctcactga tttaagagat atcaatcggt
    14341 tttcattcgt gcatatcaat accggcattc cgtttagcgt atttggggtg catctcaaag
    14401 ctggaaatac tgcgactgat aactcccgaa aagctcttga aatagacagc ttacgaaaat
    14461 ataccaatac tcttccattt ggcagcaact tggtagtttg cggtgatttt aatttttata
    14521 cctcttccga atccgggtat acaaaattat tgagcgacaa cattacctac gacggcgaat
    14581 tttatgaccc cgtcactacc atgaccggag tttggaataa tccggcttat gctatttatc
    14641 acacccagtc aacacgaaaa agtgaagtat tttttggcgg ggctaccagt ggactggatg
    14701 atcgttttga tttgattatg cattcgcagg ccgtcaaaga cccgggtgga atggattata
    14761 ttccgggcag cctgaaagca tttgggaatg atggcaatca tttcaacgat tctataaaag
    14821 cacagcccaa caccgccgtt acaccagccg tagctgatgc gctttattat gtttcagacc
    14881 acctgcctgt aattgagaaa ttcaagttca gtccggttat tttgccggtg gaattgagtt
    14941 cattttatgc gctttgcaag gacaggctga caaccttagt ttggactacc gaaagtgaaa
    15001 ccaataccgg acagtttgta ttggagcaat ctgacggcaa tgccaactgg acagccattg
    15061 acagttttcc ggcagcctat atgtctaatc tgcataaaat ctatcaaact gaattgaatg
    15121 aaacttcagg tggaattaat tactaccgtc ttaagatagc agacaccgac aatagttatg
    15181 tttattcagg tgttatagcc acaaattgta ccgaatcagc taccccttat ctccatgtct
    15241 atcccaatcc ttttaaaaat aacttgcagt tattcacgaa tatcgctgaa agcgaactga
    15301 atatcagaga tatgcttggc agaacgtaca attattcagc aacgctgata caaactctgc
    15361 ctagtggagt atatatctta ttttcgagca atcattctgt ttctccggta aaaattatta
    15421 aagaataagt agaacttact ttagacttca gctattttgt ccgctaaaaa caaataatca
    15481 tccagtattc ctttagcctt aacttctttt ccgataaatg attttagttc attattttca
    15541 aaaggattag tgcctttcag gcgcaaaaca tattcttcat ttgtcaaatt atctctgata
    15601 taaatagcat tgtgttcact cttggtacct gaaccaaact tttttacttc tactttacct
    15661 tttatcgttt ttttatttcg agacatattt tctaattccg acaatatccg aatttacaac
    15721 tttcagtact aatttctcaa tattgataac ttttccaacc tttttcgttt tggaataggt
    15781 atgaaattct acttttctgc tcgccgattt tccagcactg tgggttggtt tttttattat
    15841 atacacttta gcattgcttt tctcaaagtt gttttccttt aatgcggtat taattgctgt
    15901 gcgcaacaac gcagacgcag acgccgactt aggtttattt gtttttttag gtattcctat
    15961 cgaaattcca agttgaagtc ctttttccat cagcctatat cttctgtttt tgcgttggtt
    16021 ttgttcaatt gtcttttggg tcttaggtgc aaatgaatca gaatccggtg atacttgttc
    16081 ttcaacaaga caaacacttg ggtctccata gagtacaaac tgagccaagg tttttagttc
    16141 ttctgcatct aagtcaggtt gagcatcttc cagaaagtct aaccgagctt ccaaaaatgc
    16201 tctccctatt gatgctcctt cttttgcttt tcgcagaaaa tattgggtaa ttaaatctgc
    16261 caaaccattg ccaaccgccg gaccataagc gatattggta cttccaacat atgctaaagc
    16321 accatgaagc aaataattat tacagatagg aatctcgttt gccgtacttg catagtaaag
    16381 ttgagcgcca taacaacatt cgactgccac cattgcagca taggtaattt tattaacaac
    16441 atctgttgtg ataaatgata cgggaaagct gccatctgtt ctatctcgct gcccgtaaaa
    16501 ctcaggtgta ttgccggcac catggcagtt tatgaaatgc atcatgctcc caagttctgc
    16561 atcaccatgt gaagcagtag tatgattaaa tggcgggcaa gtttttgctg gtgtcgggct
    16621 acccgttata ttggataaac tcagattcgt acttttttcc catattttag cacttaagcc
    16681 aaaaatcgag tcgtaaacag tttttttacg gggaatattg ttaatgctat tttgaataag
    16741 cttaataagg ttatccggat ttttaatacc ggtaatatcc gggattctgc ccactactct
    16801 taccggagac gtgaacgctg atgcactagt tgaataagca gtattacatg catagggcaa
    16861 atcgctgtag ataaatctgt caatgtcatc attaggatca tagcatggat ttgtcaaacg
    16921 cacgtgtgga attatttccg tacctcctac caataatata taatcagggc taagcttcga
    16981 ataaattcca tctattgcta ttttcacctg ctctgcatcg ttaaattgtc gaactcggtt
    17041 tacgtttata gcattcatag tcgttttatc atccaataga atcagtttgg actttaaatt
    17101 ctttgttgca tctgcctgta ccaatttatt cagtgctgca ataataaatt tgctgtctgt
    17161 tttgtatttc aggtctaatg cagttttgtt ggttaaaatt actttgtcgt atttcataat
    17221 taataattta gtgaaagata caagagtcga cttagttaat gtttaacaat gattaaacgg
    17281 taagtattaa tgagcaagtg gtattaaatt cgtttttgct taccgctaaa taatctgtct
    17341 aaaactacat aagcatatga taatcaaact tttcagtctt attatcacaa aaataaaaca
    17401 ataatattat ttttagtaca atttgtaatt ttaaatagcg actgtcccaa tattgttatt
    17461 ttcctttatt ggacaaactt ttttcactct ttttttattc actttatgtt ttctataatg
    17521 tttaaaaaaa ctgattttgt tagttttagt cggtaaccta ttcagattca gtattctcca
    17581 aaatatcaac acttccaata aacagcttgt ggataataat tgataaaatt tcattttgag
    17641 tagagtagtt ttatagggtc agaagtgatt ttttggggaa aatcctgatt tttttgaaaa
    17701 tgaattaaga aaaaacatag ctatggaaga aagcattgct accgaaagaa ttttgcaaga
    17761 aagcgataac cgttttgtct tgtttccaat aaaatacgcc caaatctggg aaatgtataa
    17821 aaaacatgtg caggcttttt gggtggctga agagattgac ttgagccatg acttgattca
    17881 ttgggaaaaa ctaacagaag atgagcaaca tttcatcaaa catgtattgg ctttctttgc
    17941 tgccagcgat ggtatcgtga atgaaaacct ggttgttaat ttcatgcaag acgtaaccat
    18001 accggaagcc cgctgttttt acggttttca aattgctatg gaaaacatcc attctgaaac
    18061 ttattcgcta ttaatagaca cttatataaa agataccgca gaaaaaaaca aactcttcca
    18121 tgccattgaa accttggact gcgtaaaact gaaagcaaaa tgggcattaa agtggataga
    18181 aagcgctcct tcgtttgctc atcgcttagt ggcattttgt gccgtagaag gtatcttctt
    18241 cagtggcagt ttttgttcta ttttttggtt gagagaacga ggcttaatgc cgggtttggc
    18301 gcattccaac gagctcatta gtcgtgatga aggcttgcat tgtcagtttg cttgtttatt
    18361 atactctatg cttgaaaaca aattgagcaa agaagatatt tatgctatta tatgtgatgc
    18421 cgtagaggct gaaaaagtat ttgttcgcga ttctttgccg gttgccttaa ttgggatgaa
    18481 ctcggatttg atgtgccagt atatcgagtt tgttgccgac agctggattg ttgcattagg
    18541 ctatcctaaa gtatatcatg ctaccaatcc gtttcctttt atggaaaaca tctcattgga
    18601 aggcaaaacc aatttcttcg agcgacgcgt atctgaatac aagaaagcag gtgttggtgt
    18661 agcgaaagaa gaccaaacat tcggtattgt agaagatttt taaaaacccg tgctaagcac
    18721 gattaaaata gaataaacca tgtacgtaat aaaacgcgac ggacgaaaag aacctgtcca
    18781 ctttgataag attaccacac gcatttcccg actgtcttac gggttgagta accgctttgt
    18841 tgtgcctcat aaagtagcac aaaatgtaat tcagggattg tatgatggcg ttaccacaac
    18901 cgaattggat gaactggcgg cacgtaccag tgccagcatg acggtagtgc atccggatta
    18961 tgccgtatta gctgcacgta ttgcagtttc taatctccac aaaaacacta aaaaaacgtt
    19021 ctctcgcatt attgaagaac tgtataatta tatcgacccg aaaacaaata aaaaagcacc
    19081 gctgattgca gatgatgtgt atgaaatcat catggcaaac cgcgacagat tagatgcttc
    19141 tatcattcac tcccgagatt ataattatga ttatttcggt t
//
LOCUS       DAOQII010000012        18709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1369_length_18709_cov_6.409671, whole genome shotgun sequence.
ACCESSION   DAOQII010000012 DAOQII010000000
VERSION     DAOQII010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1369_length_18709_cov_6.409671"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..700
                     /locus_tag="PLF48_01205"
     CDS             299..700
                     /locus_tag="PLF48_01205"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNF47966.1"
                     /translation="MEIKGKITQLLPMQTGEGKNGVWRKQNIIIETDDRYPKQVCITL
                     WGNVLDELNPEVGSMLSGEVELSSREFNGKWYTDVRLWRINKGEQQSSTNASKQQSSN
                     ASHAFQNDAPEYTAKDEMTSTSAEYNDDLPF"
     gene            808..1374
                     /locus_tag="PLF48_01210"
     CDS             808..1374
                     /locus_tag="PLF48_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HNF47967.1"
                     /translation="MSENYLQSAIKQFQYYQSLGEKTMAQLNDEQLFWKPNSESNSIA
                     NIIQHLSGNMLSRWTDFLTSDGEKNWRNRDEEFEEFMHTRNELLQRWAKGWECLFTAL
                     NSLKEEDLSKIIYIRNQGHTVTEAINRQLTHYAYHVGQMVFIGKMCCDSAWQSLSIPK
                     GNSAAYNMDKFSQEKQKQHFTDEFLKED"
     gene            complement(1346..1951)
                     /locus_tag="PLF48_01215"
     CDS             complement(1346..1951)
                     /locus_tag="PLF48_01215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNF47968.1"
                     /translation="MNKFPRQTIETERLLLMPYQQKYTGEYFTLIQKNKNRLKDSFPT
                     ILRSTTSEPDTHHFIQQKIVDWNKNKAYAFLLFYKENRQLIGHLNLKALDWKKRQAEI
                     AYFIDEDFVCRGLTSEAILRVMQIVFNQLEFQRLFAKVIPSNKASVSVLEKCGLRYEG
                     AFFSDFTTYDHQQTDTNCYGITRDVFLRQKNNFNPPSKTHR"
     gene            complement(2016..3386)
                     /locus_tag="PLF48_01220"
     CDS             complement(2016..3386)
                     /locus_tag="PLF48_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF47969.1"
                     /translation="MSVNLINLMKDYLTPEVISKASSFIGENDQNTAKAADVIIPSVL
                     GGLLNKAKDENGLAAVANLLSSGGFDGNILNNVLGLFNGGENTDNVMRSGASAVTSIF
                     GDGAMSGLLNLISSNSGIQASSSNKLLNLVTPVLMGLIGKQTAGGGLKALGSLLGGQK
                     DFIAAAAPAGLASVLGLGSLSGLGGNLVNWASDAVSSATSTVTNTASTAASATKEVAE
                     DTAQAAGGLMKWLLPLILIAAAIAALLFLTKGCKGGKGDDVAGPGSDSVSIVDVATNG
                     IETTITEIKGKFDSLTGNFIYDEGAITVIQLPNNAGTLKVGENSTEAKLVAFLNDPNS
                     VVDTVKGNWFDFTNVRFKTGSSEITPESMTQLKNLVAISKGFPTAQFKVGGYTDNTGD
                     AAKNVALSQKRADAVAAQVVKLGAAKSAIVGAEGYGQEHPVCPANDTEECRAQNRRVS
                     VRVKAK"
     gene            3671..4711
                     /locus_tag="PLF48_01225"
     CDS             3671..4711
                     /locus_tag="PLF48_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HNF47970.1"
                     /translation="MKTQYFDESNKTPLVFSPDSKDNSVHYLTEWIRANHATTEQQLL
                     QHGALLFRGFDIETPQDFEAVARSIDNDLKNDYLGTSPRDKKTEYTFSASELPGHYPI
                     MQHCEMSFLPSAPRRLFFYCHVEPVYGGETPVCDFRKVYQQMDKQILAEFESKGVKHI
                     RNYSSPNDNHKSAFQLKKWNEMFLTTDKKIIEEKCRANAIEFEWLKNDGLRMTNKNEA
                     VKVHPITGEKAWFNHTQVFHVHAPVAEYRKIHQRQKRWETFKLARMLEVMTWYKKMTK
                     NPNEQSMHVTFGDDSAIPDAYVDHIIDVIWNNQSAQAWKKGDVICIDNFSTSHGRLPY
                     YGPRNIMVSWTS"
     gene            4711..5625
                     /locus_tag="PLF48_01230"
     CDS             4711..5625
                     /locus_tag="PLF48_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNF47971.1"
                     /translation="MILADTLDSVRIQVSIDGFIFLKICLAFILFSLAIDITKEDFKV
                     LVQFPKSVIIGLISQVFLLPAITFVFVYFFKPHPSIALGAFLVAACPCGNMSTYISSL
                     SKGFVPLSISITTISTLLASLTTPFNFYLYASLYTPAQPILQSIEVSFGYLLQSIFFI
                     LILPLCLGMYLRMRIPNFISRWSKVIKSAALLIFIGLLAGAFISNRKIFIQYLPGIFW
                     LVFFQNILAFFTGYVFSKLFRLPETHCRSISIETGIHNSGLGLLLTVTFFPNSGGIAL
                     LAAWWGIWHIVSGMSLAGFWHFKSVKAA"
     gene            complement(5603..6526)
                     /locus_tag="PLF48_01235"
     CDS             complement(5603..6526)
                     /locus_tag="PLF48_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47972.1"
                     /translation="MFRELYPHLEKQIAVSCLFLPSVCFWGSGILKDPICMGAIGLLT
                     YHTYKLFFTENRKVIIRVIVIIICIYVIKSIKVYIVLSFIPSLALWVIYRTRANISSG
                     FIRSVLGPVFYTLGIFIAVFVLIKFSRDAERYAVEALVRTAKDTQNWLLYSSQIQGGS
                     GYSLGNLEYTPLGLLKAAPKAINVTLFRPYLWEARKPILIPAAIEGFISLFLTLMLLY
                     KTGFVRFIKMVLANPEVQFFMVFSLVFAFSVGFTSFNFGSLVRYKIPMMPFYFMALFI
                     LADTEKKESKLVQPLKPKMKKNKNTMQLSPT"
     gene            7514..8626
                     /locus_tag="PLF48_01240"
     CDS             7514..8626
                     /locus_tag="PLF48_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47973.1"
                     /translation="MFVIALLWRKESGIVFFLFSLPLWVIADLDKKKLFSTILTLFLI
                     FIFIYLSEKMHVPYMQNNSFEKIKALDMVCARPSYYLHPSQSAPASVQLLKQWFCADE
                     LYLTGDWLAKTGELFSINFDISNLLHKIIRLIKDEQYGFLLFGLTLLSSTLSSKKHRR
                     YLLFNLIILIIFLTYLLIFYRAPKRLILPILIILSLVQFHIFITSSINMWTRYSFILL
                     ISVFCIIKTYKLFRLSEQHKQEHIKFNEINNTINSHPEYLFVANPQTIMLNYLNTLTP
                     IDSIFINGNIIIYGWINLSPDFKTYLQLFQLTNLTSDLINRDDILFINDDKDFESAYI
                     RFMKDVHHVNTEFINVNLSPLIKTKKLLLKREPSPN"
     gene            complement(8601..10013)
                     /locus_tag="PLF48_01245"
     CDS             complement(8601..10013)
                     /locus_tag="PLF48_01245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF47974.1"
                     /translation="MKYKVYNILNSKSYTNAVLLFNVLMTFSVMYFMRKEISPDHFTY
                     MYYVKGLEQGRYTYWWNFSNYIPDTFRNPGYPFLLYILAKLSDNRWVFVIAQTGIYFV
                     SIYYLLRIIKILFPKENRLVQNIFLTVLGISIVIPYYTSLIFPEITMLFLIVFTTYYL
                     LIFDKDKWQGYIVLGLLYGLMFQIRPVVLFLPFLIIAYLFIQYRGKYLYKYILMLCVY
                     VFTMLPYGCWNLKQHGVFSVTPLEGGGGAMYLGYWSYKMPGYIETRYWYNKYEKNIIS
                     FVEDKDVNNNIVAFNKEWDYIDSACAKYLTAQDTLNVKIMRRYTGSLFITYNGTYTYE
                     REKLLKKLAVQHYLDEPSYTFKVKLYTFFRLWFAGLDKNFFLNKPSFIAFVLLVLPFL
                     VTFPAFLLFVLFVPYAIFRRKIAYKEAALLLITMAYFGLIHLPFVLQSRYTIPVRPFM
                     YILISIAICRLFFNSEKVRA"
     gene            complement(10025..11257)
                     /locus_tag="PLF48_01250"
     CDS             complement(10025..11257)
                     /locus_tag="PLF48_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF47975.1"
                     /translation="MAENSAILYTSYDGMTDALGQSQVLPYLTGLSKDYGYAFHLISC
                     EKPIRFSESKAKIQEITSAAGISWHPMSYTKKPPILSTVYDLIRMYLLADKIVKKHDV
                     KMVHSRSVVSAIVGYKIAKKYGLKFIYDMRGFYADERVDGNLWNLKNPLYKWIYTYFK
                     KWETKCINESDYAVCLTHAGKSALYHWKDVRPDAKIAVIPCCSDLALFNPEKTDAKKA
                     QAYKQALGITEHSVVMSYLGSLGTWYLLDEMLLYFKSFLAKNPDAVFLFINKDDKNYI
                     ENRADYFGIKDKIRIQPAHREEVPVLLSLSKYAIFFIKPSYSKIASSPTKQGEIMAMG
                     IPIICNDGVGDTSAIIRKYQAGEVVSDVNKTDYSEEVISLDETTFDISRIKQGAEDVF
                     SLKQGVAQYAELYAKIVK"
     gene            complement(11305..13158)
                     /gene="asnB"
                     /locus_tag="PLF48_01255"
     CDS             complement(11305..13158)
                     /gene="asnB"
                     /locus_tag="PLF48_01255"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNF47976.1"
                     /translation="MCGIAGYISVNKHLEKSVFTDALQVLSHRGPDAEGVFVSDQASV
                     ILGHKRLSILDLSSAANQPYFSACGRYCIVYNGEIYNYKEIKDTLGLQTKTSGDTEVI
                     VEAFAKVGVDAFKLLNGMFAFAIYDLQDKKLYLARDRFGIKPLFLYQDNDSISFASEL
                     KFFKRLPFLNLEINYDAIPEFLHIGFIAQPYSIYRHITKFPSAHYAVLHVDNFSQIEK
                     NPYWNIKDTIQETTYSDEQQVKTKVKSLLEKSVERQLISDVPIGTFLSGGIDSSLITA
                     IAARIKQDKIKTFSIGYNFSKYDESEYAEKVAKAIGTEHYAFHVSEDEVEEILSDIIF
                     QVDEPFADSSLYPTELVSKLARNHVTVSLSGDGGDELFLGYGMYNWAERLAHPIVRAF
                     RTPLYWATRYADNKYKRGGLLLNYQELNHIRSHIFSQEQYYHSDKELRRLLLGDQFSF
                     DTINPLSGIFPRKLSPKECQSLWDIKYYLKDDLLVKVDRASMKYSLESRVPYLDNDLF
                     NYAMNIDAGLRYKGGVSKYLLKEVLYEYLPKEMFSRPKWGFSIPLSEWMRGRYKWLMD
                     KYLSPTIINRYGIMSNIFVQQLKQRFLAGEDYLYVRLWTIIILHWWLEENA"
     gene            complement(13187..14134)
                     /locus_tag="PLF48_01260"
     CDS             complement(13187..14134)
                     /locus_tag="PLF48_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF47977.1"
                     /translation="MKNIIDYRNKILVTGGAGNVGSSLAEKLISNENNYVVIVDDLTT
                     GKAEKLPSFALNARFIKADVNNYNEIAAIMLAFRFDYVFHYAAVVGVQRTLNNPVKVL
                     EDINGIKNILELSKNTGVKRVFYSSSSEVYGEPVEFPQNEDTTPLNSKLPYAIVKNVG
                     EAFLRSYQREYNLDFTIFRFFNTYGPRQSDDFVIAKFIKRALKNEDITIYGDGSQTRT
                     FCYINDNIEATVAAFEKDMFVNDTINIGNNNETSILDLAKTVIRLTGSKSEIVHLPAL
                     TEGDMTRRLPDISKMKVLLGRELTSLEQGIINILQTGLF"
     gene            complement(14136..15035)
                     /locus_tag="PLF48_01265"
     CDS             complement(14136..15035)
                     /locus_tag="PLF48_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF47978.1"
                     /translation="MPEDVSFSIIVPTYNRGHLLKQTLQSALLLDYDHYEIIVVDDGS
                     TDNTEEIMRGFSNDKVRYYKKQNEERAAARNFGVAQAKGDYVCFLDSDDFLYPHHLNT
                     ARDYILQHQQPEVFHLNYDFVDEHKNIIRRGEEFVGEDAINQRIIKGNCLSCNGVFIR
                     KDIALSYPFNTDRALSASEDYELWLRLASRFIIGYSNVITSSILSHDERSVVTLSKEK
                     MIARINKLIQYAFADKLNKKVYADKLTLFQSQCMLYLSLHLAIAKERTASLQYLFATI
                     KLRPFAVFDRRFLGIMKTLIVNI"
     gene            complement(15035..16168)
                     /locus_tag="PLF48_01270"
     CDS             complement(15035..16168)
                     /locus_tag="PLF48_01270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF47979.1"
                     /translation="MNIKFEKKNVVYVLYQIDKAPAFEWIIDSLDKNKFNLTFILLGV
                     DSGTYIENYCINKNISYYRVDYYGKKDVVKCIIKFYKLFLKIKPDIVHTHLFEACLIG
                     LTAAKLLGIKKRIYTRHHSTYHHVYFPKAVKYDKYINSLATDIVAISEVVKDVLIDKE
                     NVPPNKISLIHHGFNLSVFENTDIQLVSQMRLKYNARKQRPVIGVISRYIHWKGIEYA
                     IDAFERLLHQFPDALLILANARGQYKEAIQKKLNTIPKDNFIEIPFEIHVEALYRLFD
                     IYIHIPIDKDAEAFGQTYVESLACGIPMVATLSGVANEFLVDKKNALVVPYKDADSVY
                     NAIIEILNNPVLREQLIENGKRDVSQLFSLNLMIEKLTALYLK"
     gene            complement(16170..17078)
                     /locus_tag="PLF48_01275"
     CDS             complement(16170..17078)
                     /locus_tag="PLF48_01275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF47980.1"
                     /translation="MKDNKILFTVIVPTFNRTHMIVETINSVLVQDYPFFELIIVDDG
                     STDNTEEIVKALRDDRIVYHKKKHEERAVARNTGFQMAKGDFVTLLDSDDYLYPNHLS
                     SAESFITQHPDAEIIRFKYDIIDKKKNSLTTANIPDSINEAMIQGNYLGCSGILLSRN
                     IIVNHRFNNDLSLSGSEDYELWLRLSARYPIYTSDIVTSTLNAHAERSVIYNIQETSL
                     VNRIELLIKYSFEDKEVMRIYGKQKNDFVSKCLLYVSLHLAIAQYKKSSLKYLYKALQ
                     SKPDSLFDIRVLGILKTLLFRPELNY"
     gene            complement(17075..18214)
                     /locus_tag="PLF48_01280"
     CDS             complement(17075..18214)
                     /locus_tag="PLF48_01280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF47981.1"
                     /translation="MQKNKINNLTKVVYIISHLEKSMTFEWVLKYIDKSKFEIHVISL
                     NQHAATYLNQYCESLQIPFYHISYRSKKDIPKAIYSCLRLFQKIKADIVHAHLIDAGL
                     VGLTAATILRIKKRIYTRHYSTLHHEYYPNGVKYDHYINKIATGIIAISENVQNVLIQ
                     QEHVPASKVQLIYHGFDLSAYDDINQDRIALIKTKYNLHNKGPVIGMVSRYFKLKGIE
                     YALEAFRQYLEIYPNAVFVIANAFGPDENEIKEKLALLPEDSYREIYFEQDMIALYKC
                     FDLFIHVPINNQIEAFGLIYIEALLAELPCIFTKSGIANEVIINNQNAIVVDYKNSDE
                     ILEAMCSITEDEILKNELKNAKKRGHCNKFSVYLTVRKHENLYLL"
     gene            complement(18192..>18709)
                     /locus_tag="PLF48_01285"
     CDS             complement(18192..>18709)
                     /locus_tag="PLF48_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47982.1"
                     /translation="LLNANYHTLEGRLMDKVDNTLEFKQIDSIGSGSFVISKQLFTQI
                     GGYDESYVFQGEDIAIAQTINRHHIPILLSTQITCFHNHKDRLDIEGMMDRDYRGYYS
                     QFLQQGDDAINSTKSKSYKILLHIQNLIIKTYDLIPNSPAYDKLTFRFLGLIGNLSKT
                     KAYRDAKKQNK"
BASE COUNT         5931 a         3441 c         3101 g         6236 t
ORIGIN      
        1 aatgttcaac tttgctgcaa tgctggccac cagcaaagga acaaaaaccc gaaagccaca
       61 actggcactc aacccaatac ccatcagcaa actcagcaaa acggcatggt tgcttaagaa
      121 gcccagtgtg tctttagatg caaattgaat cagtgtacta tccattttct atgtttttac
      181 ttggtgtata tcaaactaca caccaaattt accaaataat attaacatta ctttaagcac
      241 attcaacttg tttatatctt taaaatagta ggtaacaaac agtttgtatt tttgcaacat
      301 ggaaattaaa ggtaaaatca cgcagctatt gcccatgcaa acaggagaag gtaaaaacgg
      361 cgtttggcga aaacagaata tcattatcga aacggacgac agatacccta aacaagtttg
      421 tattacctta tggggaaatg tactggatga actaaatccg gaagtaggca gtatgctttc
      481 gggcgaagta gaattatcaa gcagagagtt taatggcaaa tggtacacag acgtgcgttt
      541 gtggcgtata aataaaggcg aacagcaaag cagcacgaat gcaagcaaac agcaaagctc
      601 aaatgcaagt catgctttcc aaaatgatgc tcctgaatat acagctaaag atgagatgac
      661 atctacttcg gcagagtata atgacgacct gccgttttaa ttcatttttc ttagaacctt
      721 tcctattgta ctgcattttt taggatgtat ttgttatatt tatagtgcta attttttcag
      781 tgttactata tataagcttc actactaatg tctgagaatt atcttcaaag tgctattaag
      841 caattccaat attatcagtc tttaggagaa aagacgatgg ctcaactcaa tgatgagcaa
      901 ctgttttgga aaccaaattc agaatcgaat agtattgcca atatcataca acatttgagt
      961 ggaaatatgt tgtctcgctg gacggatttc ttaacttctg acggagaaaa aaattggcgt
     1021 aaccgagatg aggaatttga agaatttatg cataccagaa acgaattgct tcaacgctgg
     1081 gcaaaaggtt gggaatgctt atttactgct ttaaattcgt tgaaagagga agatctttct
     1141 aaaattattt atatccggaa tcaggggcat actgtaacag aagctatcaa ccggcagttg
     1201 acacattatg cttatcacgt aggtcaaatg gtgtttattg gcaaaatgtg ttgcgattca
     1261 gcttggcaat ccttgtctat tcctaaagga aattctgcgg cttataatat ggacaaattc
     1321 tcacaagaaa aacaaaagca acattttacc gatgagtttt tgaaggagga ttaaaattat
     1381 ttttttgtct taaaaaaaca tctcgtgtaa tgccataaca attcgtatct gtttgttgat
     1441 ggtcgtaggt agtgaaatca ctaaaaaatg ctccttcata tctcagtccg catttttcca
     1501 gtacgcttac agatgctttg ttggatggaa ttacttttgc aaacaagcgt tgaaactcca
     1561 attgattgaa tacaatttgc ataacacgta gtatggcttc acttgttaaa cccctgcata
     1621 caaaatcttc atctataaaa taggcaatct cggcttgtcg ttttttccag tccagcgctt
     1681 ttaagttgag atgtccaatg agttgccggt tttctttata aaaaagtaaa aaggcatacg
     1741 ctttattttt attccagtca actatttttt gctgtataaa atgatgggta tccggttcgg
     1801 aggttgtaga gcgcaaaata gtcggaaaac tgtctttgag tctgtttttg tttttttgaa
     1861 ttaaggtaaa atattctcct gtatactttt gctgataggg cattaagagc aggcgttccg
     1921 tttcaattgt ctgtcgggga aatttattca cagacaaaaa taaaaaatcc cgcgacattc
     1981 atcgcgggat aacatttcaa cttcgttgaa aaatattatt ttgcttttac acgcacagat
     2041 acacgtctgt tttgtgctct acattcttca gtatcatttg cagggcaaac cggatgctcc
     2101 tgaccgtagc cttcagcacc aacaatagca gatttagctg cgcctaattt tactacctga
     2161 gcagctactg catcagcacg tttttgagat aatgccacgt ttttagcagc atcacctgta
     2221 ttatctgtat agccgcccac tttaaattga gcagttggga atcctttaga aatggccact
     2281 aaatttttta attgcgtcat ggactccggc gtaatttcag agcttcctgt tttgaaacga
     2341 acattggtaa aatcgaacca gtttccttta acagtatcaa caactgaatt cgggtcattt
     2401 aagaatgcaa ccaatttggc ttctgttgag ttttcaccta cttttaatgt accggcatta
     2461 tttggcaatt gaataacagt aatagcacct tcatcataaa taaagtttcc tgtaagggaa
     2521 tcgaattttc cttttatttc tgtgatagtc gtttcaattc cgttagtagc aacatctact
     2581 atgcttacag aatctgaacc cggaccggca acatcgtcac ccttaccacc tttacatcct
     2641 ttagtaagga acaataaggc tgcaattgct gcggcgatta atattaatgg caataaccat
     2701 ttcatcaagc cacctgctgc ctgtgctgta tcttcagcca cttcttttgt agcagatgcc
     2761 gctgtgcttg cagtatttgt aactgtgctt gttgcagaac ttactgcatc tgaagcccaa
     2821 ttaactaaat ttccacctaa accggataaa ctgcccaaac ctaacacact ggctaatcct
     2881 gccggtgccg ctgctgcaat aaaatctttt tgtccaccca ataagctacc caaagccttc
     2941 aagccaccgc cggcagtttg tttgccgatt aagcccatta aaacaggagt tactaaattc
     3001 aataatttat tagaactgct ggcttgtatt ccgctgtttg atgaaattaa attcaacaaa
     3061 cctgacattg caccatcacc aaagatagaa gttacggcag aagccccact gcgcattaca
     3121 ttgtctgtgt tttcgccacc attaaacaag cccaaaacat tgttcaggat attgccatcg
     3181 aaaccacctg aagacagtaa attggcaaca gcagccagac cattttcatc ttttgccttg
     3241 tttaataacc cacctaatac ggaaggaata ataacatcag ctgctttagc tgtgttttgg
     3301 tcattctcgc caataaaaga actcgctttc gaaattactt cgggggtgag atagtctttc
     3361 attaagttaa taagatttac gctcattttt ttgattttat tatttttgtt aagaatgtaa
     3421 acagacagca aaattaacat ttatttaggc tgataaatgt taaaatttct tgtcatggat
     3481 gtattataat tttacacaat atgtatggca tgatttattt tttgtgtgaa attaagacag
     3541 acaacgtcat ttttcattta ctgccattta gattaaatag catgcgaatt ttttaatcgt
     3601 atcacaaagt attagtatta ccttttaatc ctgaatataa tttttgtaaa ttgtacagtc
     3661 aattttgcct atgaagactc aatattttga tgaatcaaat aaaacgcctt tggtattcag
     3721 cccggacagc aaggataatt ctgttcacta tctcacggaa tggataagag cgaatcatgc
     3781 aactacggag caacaacttt tgcagcacgg tgcactttta tttcgaggtt ttgacataga
     3841 aacgccacag gattttgagg ctgtagccag aagtatagat aatgatttga agaacgatta
     3901 tttaggcaca tcgccccggg ataaaaaaac ggaatatact ttttctgcca gcgaattgcc
     3961 cggacattat cccattatgc aacactgcga gatgagcttt ttgccaagtg cgccccggcg
     4021 tttatttttt tattgtcatg tagaaccggt atatggggga gaaactccgg tttgtgattt
     4081 cagaaaagtc tatcagcaga tggacaagca aatattagct gaatttgaaa gcaagggtgt
     4141 aaaacatatt cgaaattatt catcaccgaa tgataaccat aaaagtgcat ttcagctgaa
     4201 aaaatggaat gaaatgtttt tgacaacaga taaaaaaata atagaagaaa aatgcagggc
     4261 aaatgccatc gaatttgaat ggttaaaaaa tgatggtttg cgtatgacca ataaaaatga
     4321 ggcggtgaaa gtgcatccca tcaccggaga aaaagcatgg tttaaccaca cgcaagtttt
     4381 tcatgttcat gctccggttg cggagtaccg gaaaattcat caaagacaaa aacgatggga
     4441 aacatttaaa ctggctcgta tgctggaggt aatgacttgg tataaaaaaa tgactaaaaa
     4501 cccgaatgag cagagtatgc acgtaacctt tggcgatgac tctgccatac ctgatgctta
     4561 tgtagatcac attattgatg tcatctggaa taaccagtcg gcccaagcct ggaaaaaggg
     4621 cgatgtaatt tgtatagata atttttctac ttcacacggt cgcttgccct attatggtcc
     4681 gcgcaatatc atggtaagct ggacatcctg atgatacttg ctgatacatt agatagcgtc
     4741 aggattcagg tcagtattga tgggtttatc ttcctcaaaa tttgtctggc ttttatcttg
     4801 ttttctttgg cgatagatat taccaaagaa gattttaagg tattggttca gttcccaaag
     4861 tctgtgatta ttggcttaat ttcacaagtc tttttgctgc ccgccataac attcgtgttt
     4921 gtttattttt tcaaaccgca tccgtctatt gcactaggtg cgtttttagt agctgcttgt
     4981 ccttgcggga atatgtctac ctatatctct tctttgtcaa aaggatttgt gccattatct
     5041 atttctatca ctacaatttc taccttgtta gccagcctta ctactccgtt taatttttat
     5101 ctctacgcat ccctgtatac tccggctcag cctatcttac aatctataga agtatctttc
     5161 ggttatttat tacagtctat cttttttatt ttaattctgc cgttgtgttt aggtatgtat
     5221 ttgcgtatgc gaataccgaa ttttatttca cgctggtcaa aggttataaa gtctgctgcc
     5281 ctgctcatct ttattggttt actggcaggt gcatttatca gcaaccgaaa aatatttatc
     5341 cagtatttgc ccggaatatt ttggttagta ttttttcaga atatccttgc cttctttacg
     5401 ggatatgttt tttcaaaact atttcgatta cccgaaacac attgccgaag tatatcgata
     5461 gaaacaggca tacataattc cggtttggga ttattactga ctgtcacgtt ctttcccaat
     5521 agtggtggca ttgctttgct ggcagcttgg tggggtatat ggcatatagt ttcaggaatg
     5581 tctttagccg gattctggca ttttaagtcg gtgaaagctg catagtattt ttattttttt
     5641 tcatttttgg cttaagcggc tgaactaatt tgctttcttt tttttccgta tctgccagta
     5701 taaagagtgc cataaagtaa aagggcatca ttgggatttt atagcggact aatgaaccaa
     5761 aattaaagct ggtaaaaccg acactaaaag caaagaccaa agaaaacacc ataaaaaact
     5821 gaacttcagg attggcaagt accattttta taaagcgcac gaagccggtt ttgtataata
     5881 gcattagggt aagaaacaaa gaaatgaatc cctcaatagc agcaggaatg agaataggtt
     5941 tgcgtgcttc ccacaaataa ggcctgaata aggttacatt aatggctttg ggagctgctt
     6001 ttaacaatcc taatggcgta tattctagat tgcctaagga atagcctgat cctccctgaa
     6061 tttgcgacga atacaataac caattttgag tgtcttttgc cgtgcgcact aaggcttcta
     6121 ccgcatatcg ttcagcatct ctggagaatt taatcaatac aaatacggca ataaagatac
     6181 ccaacgtata aaatacgggg cctagaactg atctgataaa gccactgcta atgtttgctc
     6241 tcgttctata aattacccag agtgccaatg aaggaataaa tgaaagtaca atatatactt
     6301 ttatgctctt aataacatat atgcaaataa taatcacaat aactctaata atcacttttc
     6361 tattttctgt aaagaaaagt ttatacgtat gataggtcag taaaccaatg gcacccatgc
     6421 aaatcggatc ctttaatatt ccgctccccc aaaagcacac actaggtaaa aataaacagg
     6481 aaacagcaat ttgtttttca agatggggat atagttctct aaacacctta aacattttcc
     6541 aacagccata gtaacaaaaa atagtaaaag taaatgagat gagaatgtaa gaattgaaca
     6601 taaatagccc taataattgt gcaattctga ttatcaatct tgcactatcg cttgcaaaga
     6661 atgcagcacc agtcccaaaa tactggtctg catagaaatt tgaatagttt ggagaaaatg
     6721 ccaaatttaa ataagcccct gggttttcca ataaaatact acgtaaactg ctttcatatt
     6781 gaaaataggt acaggtgtct ccacctttgt aatagaactg atatattagt gctaagaaga
     6841 ttgcagaaca taatcgtatt gacaatccca gaacaaaata ttttgaataa atatcatcag
     6901 attttaattt tacatacctt tgagcaataa acaaaataac aaaaaagaac attggtccta
     6961 aaatgagatc ccttaagaac aaaaactgag tatgttctcc tgtatattgt aatatattca
     7021 aattctcaga ttacagccta taatgaattg taaatatact aaatatgtct tacctgctgt
     7081 actcatctta acattgatgc cattatttat ttttcacctt tctttcaaca ataatgatga
     7141 tcagctcatg ttcttgtttt caaatgggat gagcagtaaa tttcaaagta ataatctttt
     7201 actgatacac cctgttttaa gctttttgat taataagtgg aatggtatca cttctgccag
     7261 aacaaataca tacagtttgt ttttatacac tacactctct acgaatataa tattgctttt
     7321 tgtttatttt gcgcaatgca acagcattaa aaaattacct gaaaccctcg tgatggtcat
     7381 tttcattttt cttggttatt tcctttattt ttatttagaa ctagaattta cttcagtatc
     7441 tttactctta gtactgtctg tgctttattt cattattacg aacaagcaac aaaacacaat
     7501 tttgtatatc atcttgtttg ttatagcttt actttggcga aaagaaagtg ggattgtttt
     7561 cttcctcttt agtttgccat tatgggttat cgctgatttg gataagaaga aattattttc
     7621 aacaattttg acgttatttc tcattttcat tttcatctat ttaagtgaaa aaatgcatgt
     7681 tccttatatg cagaataaca gttttgaaaa aataaaagct cttgatatgg tttgtgccag
     7741 acctagttat tatctgcacc catcacaatc tgcgcctgcc tctgtacagt tattaaaaca
     7801 gtggttctgt gcagatgaat tatatttaac tggtgactgg ttagcgaaaa caggagaatt
     7861 gtttagtata aattttgata tcagtaattt attacataag attattagac ttataaaaga
     7921 tgagcaatac ggttttttgt tatttggact tacattactc agtagtactc tgtccagtaa
     7981 aaaacacagg cgatatttgc tgtttaatct aattatatta attatatttt tgacttattt
     8041 actcattttt tacagagctc ctaagcgttt gattctgccc atacttatca tactatcttt
     8101 agtgcagttc catatattta ttacttcaag cataaacatg tggactcgat actcgttcat
     8161 tcttttaatt tcagtttttt gtataatcaa gacttacaaa ctctttcgac tgtcggaaca
     8221 acacaaacaa gaacatataa aatttaatga aataaataat acaataaaca gtcatcccga
     8281 atatctgttt gttgccaatc cgcaaacaat catgctcaat tacttaaaca cacttacacc
     8341 catagacagt atatttataa atggaaatat aattatttat ggatggataa acttgtctcc
     8401 ggattttaaa acttacttgc aattatttca gttaactaac ttaaccagcg atctcataaa
     8461 cagagatgat atcctattta taaatgatga taaagatttt gaatctgcat atataagatt
     8521 catgaaggac gttcatcatg ttaatacaga atttataaat gtaaatttgt ccccgttaat
     8581 aaaaacgaaa aaattattgc ttaagcgcga accttctccg aattaaaaaa tagtctgcaa
     8641 atagcaatgc ttattagaat atacataaat ggtctaacag gaatagtata tcttgattgt
     8701 agcacaaagg gcagatgtat aagaccaaag taagccattg ttatcaaaag caatgctgct
     8761 tctttataag caatctttcg cctaaaaata gcatacggta caaagagaac aaacaataaa
     8821 aatgccggaa aagtaactag aaaaggtaaa acaagtaaca caaatgcgat aaaacttggc
     8881 ttatttaaga agaagttctt atctaatcca gcaaaccata atcgaaagaa agtatacaat
     8941 ttcactttaa aagtgtatga tggctcatca aggtagtgtt gaactgcaag tttctttaat
     9001 aatttttctc tctcataagt atacgttccg ttataggtaa taaataggga accggtatat
     9061 cttctcataa tttttacatt tagtgtatcc tgagctgtta gatatttggc acaagcagaa
     9121 tcgatataat cccattcttt attaaatgca acaatattat tatttacatc tttatcctct
     9181 acaaaactaa taatgttttt ttcatatttg ttataccaat atctagtctc aatatatcct
     9241 ggcattttat agctccagta gcctaagtac attgccccac cgccgccttc taatggagtg
     9301 acggaaaaga ctccatgttg ctttaaattc cagcaaccat agggcaacat tgtaaaaaca
     9361 tacacacata acatcaatat atatttgtac aaatacttgc ctctgtattg gataaacaga
     9421 taggcaataa tgagaaaagg caaaaacaaa acaactggtc tgatttggaa catcaggccg
     9481 tacaataatc ctagaacaat atagccctgc catttgtctt tatcaaatat taatagatag
     9541 tacgtagtaa atactatcag aaacaacatc gttatttcag gaaaaattag ggatgtataa
     9601 tatggaatga ctatcgaaat gcccaagaca gtaagaaata tattttgaac caatctgttt
     9661 tcttttggaa aaagaatttt aataattctc aataagtaat aaattgagac gaagtaaata
     9721 ccggtttgtg caattacgaa tacccatctg ttgtctgata attttgctaa tatatataat
     9781 aaaaaagggt agcctggatt tctgaaagta tctggtatat agttactgaa attccaccaa
     9841 taagtatacc gaccttgctc taaacctttt acataataca tatacgtaaa atgatcgggt
     9901 gatatttcct tacgcataaa atacatcact gaaaatgtca ttaaaacatt aaagagtagt
     9961 acggcattgg tatacgactt gctatttaaa atattatata ctttgtattt caatctgtaa
    10021 aattttattt tactatcttt gcatataact cggcatactg tgccactcct tgttttaatg
    10081 aaaaaacatc ttctgctcct tgttttatac gcgaaatatc aaacgtagtt tcatccagac
    10141 ttattacctc ttcggaatag tctgttttgt ttacatcgct aaccacttcg cctgcctgat
    10201 attttcttat aatggctgag gtatctccta ctccatcatt acaaataatg ggaataccca
    10261 tagccatgat ttcgccttgt tttgtaggag aagaggctat tttagaataa cttggtttaa
    10321 taaagaatat tgcatatttg cttaatgaaa gtaacaccgg tacttcttct ctatgtgccg
    10381 gctgtattcg tattttatct tttattccaa agtaatctgc tctgttttca atataatttt
    10441 tgtcatcctt atttataaat aaaaaaactg catccggatt tttagctaaa aaacttttga
    10501 aatacagtaa catttcatcc aagagatacc atgtccctaa tgagcccaga tagctcataa
    10561 cgacactgtg ctcagtaatt cctaaagcct gtttatatgc ttgtgccttt ttcgcgtctg
    10621 tcttttccgg attgaataac gccaagtctg agcaacaagg aattacagct atcttagcat
    10681 ccggccgcac atccttccaa tgatacaaag ctgatttgcc ggcatgcgtc aaacaaacag
    10741 cataatcact ttcgttaatg cattttgttt cccatttttt aaaataagta taaatccatt
    10801 tgtacaaggg gtttttaaga ttccacaaat tgccgtctac ccgctcatct gcataaaatc
    10861 ctcgcatgtc ataaataaat ttcagcccgt atttttttgc aattttatag cccacaatgg
    10921 cggacacaac agatcggcta tgcaccatct taacatcatg cttttttaca atcttatctg
    10981 ccagcaaata cattctgatt aaatcatata ctgtagaaag aatgggtggt tttttggtgt
    11041 atgacattgg atgccagcta attcctgctg ccgaagtaat ttcttgaatt ttagctttgc
    11101 tttcagaaaa cctaattggt ttctcacaac ttattagatg aaatgcatag ccatagtctt
    11161 ttgataaccc tgttagatag ggcaatactt gtgattgacc caaggcatct gtcatgccat
    11221 cataagaagt ataaagtata gctgaatttt ctgccacttt agttgaaaat aaccgaataa
    11281 atagttgtgt ttagcatgct aaagttaagc attttcttcc aaccaccaat gcagaataat
    11341 aattgtccac aagcggacat acagatagtc ttctcctgcc aaaaagcgct gcttcagctg
    11401 ttgaacaaat atattgctca ttattccata tcggttgatg atggtaggac tcagatattt
    11461 atccatcagc catttatacc tgcctcgcat ccactcggaa agcgggatgg aaaaccccca
    11521 tttgggtctt gaaaacattt ctttgggtaa atattcgtag agtacttctt tcagtaaata
    11581 tttagatact ccccctttat acctcaagcc tgcgtcaata ttcatggcat aattaaacaa
    11641 atcattgtct aagtatggta ctctgctttc taatgaatat ttcatggaag cacggtcaac
    11701 tttaactaat aaatcgtctt ttaaataata ttttatatcc catagagatt gacattcttt
    11761 tggagataat ttgcgaggaa aaattcccga taatggattt atcgtatcaa atgaaaactg
    11821 atcacctaat aatagtcttc tcaattcctt gtctgaatga taatattgtt cttgcgaaaa
    11881 aatatgactg cgaatatgat ttaattcctg ataattaagg agcaaaccac ctcttttata
    11941 tttattgtct gcatagcgag tcgcccagta taacggtgtg cgaaaagctc gcacaatggg
    12001 atgtgccaat ctctccgccc agttatacat gccatatccc aagaaaagct catctccacc
    12061 atctcccgag agcgaaacag ttacatgatt acgagctaat ttagatacca actctgttgg
    12121 atacaaagat gagtcggcaa agggctcatc cacctgaaat attatatctg acaggatttc
    12181 ttctacctca tcttcagaaa catgaaaagc ataatgttca gtaccaatgg ctttggctac
    12241 tttttcagca tattcagatt catcatattt tgaaaaatta tatccgatgc taaaagtctt
    12301 aattttatct tgtttaattc ttgcagctat ggccgttatc aagcttgagt caatacctcc
    12361 gcttaaaaaa gtaccaatag gtacgtcgct tatcaactgc cgttctaccg atttttccag
    12421 caaggatttc accttagtct ttacttgctg ttcatccgaa taagtagttt cttgtatggt
    12481 gtcttttata ttccaatatg gatttttttc aatttgggaa aaattatcta catgaagtac
    12541 agcataatga gctgatggaa attttgtaat atgacgataa atgctgtaag gctgtgcaat
    12601 aaagccgata tgcaagaatt ccggaatggc atcataatta atttccaaat tcaaaaatgg
    12661 caatctttta aaaaacttca gttcagaagc aaaagagata ctatcattat cttggtagag
    12721 aaaaagcggc tttataccaa accggtcacg tgccagataa agctttttat cttgtaaatc
    12781 ataaatggca aaggcaaaca tgccattgag taatttgaaa gcatcgacac ctacctttgc
    12841 aaatgcttca actatcactt cggtatcgcc acttgtttta gtttgtaagc ctaaggtgtc
    12901 tttgatttct ttataattgt atatctcgcc attataaacg atacaatatc ttccgcaagc
    12961 tgaaaagtaa ggttgattgg ctgcactaga taaatctaat atacttagac gcttatgtcc
    13021 taaaatcacg cttgcctgat cagaaacaaa aactccttcg gcatcagggc ctctgtgact
    13081 caatacctgt aacgcatcag taaatactga tttttccaaa tgtttattta cagagatata
    13141 tcctgctatt ccgcacataa cttataatct gataaagata taacttttaa aacaatccgg
    13201 tttgtaaaat atttattatt ccttgctcta atgaagttaa ctctcttccc aataatactt
    13261 tcatcttgga tatatcgggt aagcggcgtg tcatatctcc ttcagtaagt gccggcaaat
    13321 ggactatttc agattttgag ccggtaagcc ggataacagt cttggccaaa tcgagtatac
    13381 ttgtttcatt attattgcca atattaattg tatcattcac aaacatatct ttttcaaagg
    13441 cagctacggt ggcctctata ttgtcattaa tataacagaa ggttcgggtt tgtgaaccat
    13501 ctccatagat agtaatatct tcgtttttta atgctctttt gataaatttt gcaatcacaa
    13561 aatcatctga ctggcgcgga ccatacgtat taaagaaacg gaagatggtg aaatccaaat
    13621 tatattctcg ttgataagag cgcaaaaagg cctcacctac attttttaca atagcataag
    13681 gtagtttgga gttgagtggt gtagtatctt cattttgcgg aaattctacc ggctcgccat
    13741 atacttctga tgatgatgag tagaagactc tttttacgcc ggtatttttt gagagttcca
    13801 aaatgttttt tatcccattt atatcttcca gaacttttac gggattgttt aaagtacgtt
    13861 gtactccaac tacagccgca tagtgaaata cataatcaaa acgaaatgcg agcataatgg
    13921 ctgctatttc attatagtta ttaacgtctg cttttataaa ccgtgcattg agggcaaagg
    13981 aaggcagttt ttctgctttt ccagtagtaa gatcatctac gattactacg taattattct
    14041 cattactgat gagtttttct gccaatgaac ttcccacgtt tcctgctccg cctgttacta
    14101 atattttatt ccggtaatct attatattct tcatgctaaa tatttacaat aagtgttttc
    14161 attatcccta aaaatctgcg gtcaaaaact gcaaatggtc tcaattttat tgtggcaaat
    14221 aaatactgca aagatgcagt tctttctttt gcaatagcta aatgcaatga caagtacagc
    14281 atacattgcg actggaataa agtcagttta tctgcatata cctttttatt gagtttatca
    14341 gcaaaggcat attgtataag tttattgatg cgcgctatca ttttctcttt cgaaagcgta
    14401 accacacttc tttcatcatg actgagtatg gatgaagtga tgacattgga ataaccgata
    14461 ataaaacgcg atgccaaccg caaccatagt tcataatctt ctgaagctga cagtgccctg
    14521 tcagtattaa aaggataact caacgctata tctttacgaa taaaaacccc attgcaactc
    14581 aggcagtttc ctttaataat gcgttgatta atggcatctt caccaacgaa ttcctctcct
    14641 cttcttataa tatttttatg ttcatccaca aaatcataat tgagatgaaa gacctccggt
    14701 tgctgatgtt ggagtatata atcacgggca gtattcaagt gatgtggata caaaaaatca
    14761 tcagaatcaa gaaaacaaac atagtcccct ttggcctgtg ccacgccaaa atttcgggct
    14821 gctgcccgtt cttcattttg tttcttatag tatcttacct tatcatttga gaatccgcgc
    14881 ataatttctt cagtattgtc ggtactcccg tcgtcaacta caataatttc gtaatggtca
    14941 taatctaaaa gtaaagcact ctgcaaggtt tgcttaagca aatgccccct gttgtaggtg
    15001 ggcacaatga tagaaaaaga tacatcttca ggcattactt cagatagagc gctgttaatt
    15061 tttcaatcat caaatttaag gaaaaaagtt ggcttacatc acgctttcca ttttcaatga
    15121 gttgctctct caaaactgga ttattaagta tctctataat ggcattatat accgaatcag
    15181 cgtctttata tgggacaaca agcgcatttt ttttatcaac aagaaattca ttagcaacac
    15241 ctgacaaagt agcaaccatt ggtataccgc atgctagaga ttcgacatag gtttgtccaa
    15301 atgcttctgc atctttatca attggaatat gtatatatat atcaaaaagt ctatataatg
    15361 cttccacatg aatttcaaat ggtatctcta tgaagttgtc ttttggaata gtatttaatt
    15421 ttttttgaat tgcttcttta tattgtccac gagcatttgc taatatgagc aaagcatcag
    15481 gaaattgatg taataatctt tcaaaagcat caatggcata ttcaattcct ttccagtgga
    15541 tatacctgga aatcacacca atcacgggac gttgtttgcg tgcattgtat ttcaatcgca
    15601 tttgacttac taactgtata tctgtatttt caaatacgct tagattaaaa ccatgatgaa
    15661 ttaatgagat tttgttggga gggacatttt ctttatctat taacacgtct tttacaacct
    15721 ctgatattgc aacaatatca gttgcaaggc tattaatata cttgtcatat ttaacagctt
    15781 taggaaaata tacatgatga taggtagaat ggtgacgtgt gtaaatgcgc tttttaatgc
    15841 caagtaattt tgcagccgtt aatccaatta aacaggcttc aaataagtga gtatgtacaa
    15901 tgtccggttt tattttcaga aataatttgt aaaattttat tatgcatttc acaacatcct
    15961 ttttcccgta ataatcaact ctgtaataag atatgttttt attaatacaa taattttcaa
    16021 tataagtgcc agagtcaact cccaataata taaaagtcag attaaattta ttcttatcta
    16081 aactatcaat tatccattcg aatgccggtg ctttgtcaat ttgatacaat acatagacaa
    16141 cgttcttttt ctcaaactta atattcattt taatagttta actcaggtct gaaaagcaat
    16201 gttttcaaaa tacccaaaac ccgaatgtcg aataatgaat cgggtttgct ttgcaatgcc
    16261 ttatagagat acttcaatga tgacttttta tactgggcta tggctaagtg cagcgataca
    16321 tatagcagac acttagaaac aaaatcattt ttttgttttc cgtagatgcg catcacttct
    16381 ttgtcttcaa aagaatattt aatcagtagt tcaattctgt taaccaatga tgtttcctga
    16441 atattataaa tcacacttct ttctgcatgt gcattcaaag tactagttac gatatcggag
    16501 gtatagattg gatatctggc agaaaggcgc agccagagct cataatcttc agaccctgat
    16561 agggatagat cattattaaa gcggtggttg acaatgatat ttctggaaag caagatgcct
    16621 gagcatccca aatagtttcc ttgtatcatg gcttcattaa tgctatcagg aatattggct
    16681 gtggtaagag agttcttttt tttatcaata atatcatact tgaagcgaat tatttccgca
    16741 tccggatgtt gtgtgataaa cgattctgca gaagataaat gatttggata caggtagtca
    16801 tccgaatcga gcaaggttac aaaatcacct tttgccattt ggaaaccagt attccgggct
    16861 actgctcttt cttcgtgttt tttcttatgg tagacaattc tgtcatcacg taaagctttg
    16921 acaatttctt ccgtattgtc tgtgctgcca tcatctacta tgataagttc aaaaaaagga
    16981 taatcttgta ccaatacact attgatagtc tccactatca tgtgagtgcg gttaaaggtt
    17041 gggacaatga cagtaaaaag aattttgttg tctttcatag taggtataaa ttctcatgtt
    17101 tccttactgt caaatataca gaaaacttat tacagtgtcc tctttttttt gcgttcttaa
    17161 gttcattttt tagtatttca tcttccgtga tgctgcacat tgcctccaga atttcatcac
    17221 tgtttttata atcaaccaca attgcatttt gattatttat aataacttca ttagcaattc
    17281 ctgattttgt aaatatacag ggtaattcag ccaataaggc ttcgatataa ataagtccaa
    17341 atgcctcaat ttggttattt atcggtacat gtataaacaa atcaaagcat ttgtaaagcg
    17401 caatcatgtc ttgttcaaaa tatatttctc ggtaagagtc ctcaggaagc aatgctaatt
    17461 tttcttttat ttcattttca tccggaccaa atgcattcgc tatgacaaaa actgcattag
    17521 ggtatatttc caaatattga cgaaacgctt cgagtgcgta ttcaattcct tttaacttaa
    17581 aatatctgga taccatgcca ataaccggac ctttattatg taaattgtat tttgttttta
    17641 taagtgcgat tctgtcttga ttaatatcgt cataagccga taaatcaaag ccgtgataaa
    17701 tgagctgaac ttttgatgcg ggcacatgtt cttgctgtat cagcacattc tgcacatttt
    17761 ctgaaatagc gataatacca gttgcaatct tattaatata atggtcatac tttactccgt
    17821 tcggataata ttcgtgatga agtgtagagt aatgcctcgt ataaattctt tttttaattc
    17881 gcaaaatagt tgcagctgtc agccccacta atcctgcatc aattaaatgg gcatgtacta
    17941 tatccgcttt tattttttga aataatcgta aacaactata aattgctttg ggtatatctt
    18001 ttttactgcg atatgaaata tgataaaatg gaatttgcaa cgattcacaa tattgattca
    18061 aataagtagc tgcatgttga ttcagactaa tcacatgtat ttcaaatttc gatttgtcaa
    18121 tatatttcaa gacccactca aaggtcatcg atttttcgag atgtgaaata atatagacaa
    18181 cctttgtcag attatttatt ttgttttttt gcatctcgat atgctttcgt tttacttaaa
    18241 tttccaatta gccctaaaaa cctgaacgtt aatttatcat acgccggact atttggaatc
    18301 aaatcatagg tcttaataat caaattctga atatgtaata atattttgta ggatttactc
    18361 ttagttgagt ttattgcatc atctccttgt tgaagaaact gtgaataata tcccctgtaa
    18421 tccctgtcca tcataccttc aatatcaagt ctgtctttat gattgtggaa acatgttatc
    18481 tgagtcgaca ataatatcgg aatatgatgt ctgtttattg tttgagctat tgcaatatct
    18541 tctccctgaa aaacatatga ttcgtcgtag cctccaattt gtgtaaatag ctgcttactg
    18601 attacaaatg agccgctccc tatagaatca atttgcttaa actcaagcgt attatcaact
    18661 ttatccatca atctgccttc caaagtatga taatttgcat tcaataaat
//
LOCUS       DAOQII010000013        18351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1417_length_18351_cov_5.837943, whole genome shotgun sequence.
ACCESSION   DAOQII010000013 DAOQII010000000
VERSION     DAOQII010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18351
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_18351_cov_5.837943"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="accB"
                     /locus_tag="PLF48_01290"
     CDS             complement(<1..435)
                     /gene="accB"
                     /locus_tag="PLF48_01290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNF47983.1"
                     /translation="MDIAQIKKLIQLVNDNNVGELKVEEGDLKISIKHRDFIKQTVVS
                     GASVSAPVAQLQSLPAPSNEVKQENTTAESSSAIAIKAPMIGTFYRSSSPDMPAFVKV
                     GDVIKKGDVLCIIEAMKLFNEIQSEVSGKIVKVNADNAQPVEY"
     gene            complement(536..1252)
                     /locus_tag="PLF48_01295"
     CDS             complement(536..1252)
                     /locus_tag="PLF48_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47984.1"
                     /translation="MRQIHFDKIQRHGLLLFFMVTFFVTTFSKEYVIHYKLLNYDTLD
                     HFYFQVNDTIVFEDTLGVNKSGKFSVNIEEPGKMIFCANDDYRHIKRCFLDLRNASVI
                     IEKNKLTVDIPNSKLNDAYNISLIIQDSFRTTVERPIENLLPKNPNMIISDSILSVID
                     SLEIELDKIIFKRLHYKSYIVLHRMCLDALHYIKHRQMLIEDFNKLHPSLKKYREYDE
                     IKRLLYKVEDVIAPPLFKIE"
     gene            complement(1421..1612)
                     /locus_tag="PLF48_01300"
     CDS             complement(1421..1612)
                     /locus_tag="PLF48_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47985.1"
                     /translation="MLYISDKMKPTKKYKKAGTKNKIVQENTLITELVDPVKFPVALM
                     PELDELVKKNPNRLLGCGG"
     gene            1642..2676
                     /locus_tag="PLF48_01305"
     CDS             1642..2676
                     /locus_tag="PLF48_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF47986.1"
                     /translation="MKWIAKYKGFLRRVRFLQYIYNLFHKKGLEHNKHFYNLYQIKKS
                     IYDSVSHRDFKNLRLKSPWMDRIVGQNEILNHPEYIRFPEAVEEQLLHWHKTGFLIWE
                     NFLDEETINSINFELDELIGKNKVQFNYTSRKIFNAYKHSYTIRKVIKDKRLLQLFHF
                     IMERNAIPFQTINFMKGSEQKAHSDSIHMSTFPEGNLVAAWFALEDIGDMQGPVFYYP
                     GSQDLPYIYNEDYPNDNTRFLLDENANEKYEAKVQEVLNNHPELKKEIYMPKKGDLLI
                     WHANLLHGAEPIKDYNLTRKSMVVHYFGEDVVCYHEISERPTIFDTELVGEVKEKFFK
                     GDSDVFDIGE"
     gene            complement(2677..3006)
                     /locus_tag="PLF48_01310"
     CDS             complement(2677..3006)
                     /locus_tag="PLF48_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47987.1"
                     /translation="MENENKIQEYTLQLIEETGLSFSSFEEFRKYIIQKINYWINNDF
                     EHLLFMLYRIDVHEDKVRRLMAEHKGENASEIIADLIIERQRQKIETRKLFKDFKPDE
                     GDESELW"
     gene            complement(3078..4667)
                     /locus_tag="PLF48_01315"
     CDS             complement(3078..4667)
                     /locus_tag="PLF48_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNF47988.1"
                     /translation="MLTHSIYQIYPRSFYDANGDGMGDIQGIIQKLDYISDLGFETIW
                     ISPFFASPQRDFGYDISDYFSIAPVYGTMRDAEQLIDEVHQRGMKVMFDMVLNHTSDE
                     HVWFKESASSRDNTKADWYIWRDKPNNWKSIIGPSGWHYCDSRKQYYYASFLPFQPDL
                     NYHNKEVKQQMWEVCRFWLSKGVDGFRLDIFNCIIKDKEFRDNPFSLFHMIPSEEYPG
                     GNFQLRKYSVNQPENFELAKELRKVADEFSPPRILLGEVFGTQETISRYLGNNDGLHL
                     IFSFDILFFKFKASFFKNILQQYEQNFPHPKIPTVVFSNHDQWRSQGRLNNDLEKAKL
                     LAVLQMTIRAIPVVYYGEEIGMQNGDIAIRNAKDSLAKPYKWIPQFLVNKLPVAINRD
                     NCRTPMQWNNGKHAGFSSAEKIWLPVNANYTERNVASMQTDKNSLLSVYKELLFLRKN
                     ENALLLGNMSRLDILDKSILVYERTHEKERMKIYLNFSRKKKKIPKLATGENIRLLIG
                     EVVHEHDRICMGKYAALIVKY"
     gene            4731..5228
                     /locus_tag="PLF48_01320"
     CDS             4731..5228
                     /locus_tag="PLF48_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47989.1"
                     /translation="MKNSFVYIFIAICIVFAGISSCSVGNKEIPNPTGNQIVDTTGMS
                     TINLNVSGDTSYSLHLKVYADTVVNSVLFVSGTDYTTQDSVTFATLKDTVGVYIPNLP
                     SGLFVFSKKQTSGHVRYPLTQGSISITQRDTSTRIYQGVINVNNNNTSGQPHYTISGN
                     FVVKN"
     gene            complement(5225..6412)
                     /locus_tag="PLF48_01325"
     CDS             complement(5225..6412)
                     /locus_tag="PLF48_01325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF47990.1"
                     /translation="MTKKLLFLTPTLPYPPVSGGVIKSNKTVRFLAEHCSVSVACFLK
                     NDDNLYRDEFLRSLNLENDYMEELNIPRSAKNLILSNLKGIPLNLYRNASASFAKKIK
                     AWIHDFDVVFCDHYVMFQYIPDDFRGKIVLHEHNCEYLIWQRYADVEPRFFKRLALLN
                     QAWRIKNYERQICNRSDAILAAPNDTDELVKIGADRSKFFETYHLGDDATLALPDLQF
                     AQTEKALLYIGTLSWEANIDGLLWFYREVWGKLVTQEPDLKLYIIGKHADKRLLALQA
                     LDTRIILTGFVEDLEPYFQKCRVFITPLRFGSGIKVKVVNSLYRGIPCVTTTIGVEGL
                     KVTDGKDIIVRDNARQQADAIIELLHNKAQWESISTNARTLAEEFYTWDAVLENIQKA
                     VES"
     gene            6514..7644
                     /locus_tag="PLF48_01330"
     CDS             6514..7644
                     /locus_tag="PLF48_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF47991.1"
                     /translation="MLLPLEYHFPGKEIKQHTLDRYLASGYFRTGNYLLRTRVLYYDN
                     QILNTLHIRIRLAKHILPKRLNKLLQKNNRLFSWNIKPFCITEEKEKLYQMHRKRFSS
                     NTSPSLSAYMFDHFNKNLFETYEINIYRNNQLIGYSIFDKGQVSMASILGIFHPDYAA
                     YSIGMYTMLLEIEFAKTEGYKYYYPGYVAYEPSKFNYKLRLSDVFDYYDWYSKRWMSF
                     SKKDTRIKVNDYFEQQLNHAKVWLDELGIAYDECFYPYFYMGGMYPQSDCVKGVKHLL
                     IRNFQIKGVYYILEFHPEKMQLLLSGITVHKYQFEDDIDFDEVLEEKRWKRVLLYLHP
                     SVKITSSQDLYAAYLFLQELFTANIQPPPIPNTSFKNPNRNR"
     gene            complement(7652..8116)
                     /locus_tag="PLF48_01335"
     CDS             complement(7652..8116)
                     /locus_tag="PLF48_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNF47992.1"
                     /translation="MTSKAATVEQYLSEIDDPDKKIMAEKLRTTILKHLPKGFKECMN
                     YGMIGYVVPHSLYPKGYHCTPELPLPFMSIGVQKNSVNYYHMGIYMKQELLDWFVNEY
                     HKNYKAKLDMGKSCMRFKKEGNIPYALIGELVKKLSTDEYIQLYESILNKKK"
     gene            8214..8810
                     /locus_tag="PLF48_01340"
     CDS             8214..8810
                     /locus_tag="PLF48_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF47993.1"
                     /translation="MDLLQALPSAQRVLGHYNINFLSAKEWSTLIQFGQLKSFKKGEN
                     FITANQINTKIAFILKGGFRFFYLKNETEITCLFAFENDMIGSFECNILKQPCTQTIQ
                     ALEDSDVFIIDYKDLESLYDKSQKFEKVGRIILEHYMAFLQLRITSYLLDTPEERYLR
                     LIHETPDLLNRVPLQHIASFIGVTPVSLSRIRKRILKK"
     gene            complement(8799..9221)
                     /gene="ybeY"
                     /locus_tag="PLF48_01345"
     CDS             complement(8799..9221)
                     /gene="ybeY"
                     /locus_tag="PLF48_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNF47994.1"
                     /translation="MAIHFYFDAVKKPAFAKPVQWKKALKRIAEVEAYKIKELTYVFL
                     TDEELLAMNIQYLQHDTYTDIITFDNSSEPKSIEGDIFISLERVQDNARKYKVSYEVE
                     LQRVLAHGLLHLCGYKDKTKKDAALMRQKEETAIQLFL"
     gene            complement(9254..9916)
                     /locus_tag="PLF48_01350"
     CDS             complement(9254..9916)
                     /locus_tag="PLF48_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF47995.1"
                     /translation="MQGIRNYFISGIATDERAFHLQYDCIPNAVYLPFPKHEKKDKMS
                     DYARRFIPLINTGEPFNLIGHSMGGIITMEIIKHIHPEKVILISSVKSRKEMPFNLKQ
                     LKITHFHKLLPGKGFIKSIEVGSKFIKEIMQTPGLRELAISMAKNNDPSFLYWAVNAI
                     VKWNGDENYRQDIIHLHGTKDTMFPYKNIKNPIPVFGGTHEMNIIKAEEVNRLILYYL
                     NK"
     gene            complement(9918..10580)
                     /locus_tag="PLF48_01355"
     CDS             complement(9918..10580)
                     /locus_tag="PLF48_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNF47996.1"
                     /translation="MLTAVEENYLKYIFQLLDDLDTETVKTNDLAYKLEHSPATVTDM
                     LKKLSDKKLVQYKKYYGASLTKVGRQTAIKVLRKHRLWETFLVRNLKFTWDKVHEIAE
                     QLEHVQSDELVERLDEFLGFPKFDPHGDPIPDKHGYFHFAQQIALTDAKLKENYILAG
                     VNDASTAFLVYLNKIKIQLNDKIRIIEKEEFDDSVTIVVNDRKTITISHSAAGNMWVK
                     KV"
     gene            10655..11983
                     /locus_tag="PLF48_01360"
     CDS             10655..11983
                     /locus_tag="PLF48_01360"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNF47997.1"
                     /translation="MAANDKKWLVPDSDFSLPESYATIAVPKKSGFFRKLLAFTGPGL
                     MVAVGYMDPGNWATDIEGGSMFGYTLLSVILISNLFAILLQYLSLKLGISTGKDLAQA
                     CRAAYSPKVSFALWIAAEIAITATDLAEVIGSAIAIQLLFGIPLPIGVLITAVDVLLI
                     LFLQQKGFRYLEIIVGGLIGIILLCFLTELLLSRPHINEIVHGLIPSTAIITNQDMLY
                     IAIGILGATVMPHNLYLHSSIVQTRQYERNDEGKKEAIRFATIDSTLSLMLAFFINAS
                     ILILAASVFHFNQHTDVADIYDAHKLLEPVLGNRLASVLFAVALLASGQNSTLTGTLS
                     GQIVMEGFVNLRLRPWLRRLITRLLAIIPAMGVAVYYGESGTGKLLVFSQVVLSMQLS
                     FAVVPLIRFTGNTTLMGKFVNKPLTKILAWIIALIIISLNAFLLVNFFFT"
     gene            complement(12159..12638)
                     /locus_tag="PLF48_01365"
     CDS             complement(12159..12638)
                     /locus_tag="PLF48_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF47998.1"
                     /translation="MKKLFTIFILSTIAYCASAQEISVQKIEGLKALYNRNNDTTYIV
                     NFFATWCRPCMEEMPVLKKFIEEHQAEKIQFIFVSLDGGGAITKLQKYVKKQKITVPV
                     ILLNESSDFSWLPYIDKRWQGSIPATKVVNASKKVNAFLETPLEKGQLEYYLKQLGL"
     gene            12709..13629
                     /locus_tag="PLF48_01370"
     CDS             12709..13629
                     /locus_tag="PLF48_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF47999.1"
                     /translation="MNEEILDMPLIEKPEKRITAFDWAVFIGLSTIWGFSFFFIKKGL
                     EVFEPVQVAAFRMTIAFLSLTPMFFVHAKQLKLQAHQWKMVPLLGLFGNMIPAICFCV
                     AGTKIDSALSGIMNSTTPLFALLIGGLFFGVMLTRNKILGVLLGFVGAIIIVLSKQKG
                     IHSINWYVLLPLLATICYGLNANIFKAGFQQNNPVVIALWQYAFVAFFSFIYLWYSGA
                     FHLLFTNFGKAWHSLKYLLLLGIFGTGIAQVFFNVLAQRTSALFATMTTYLIPMVAIL
                     VGLFIGEKIHILHLIGLAIILSGIYIGSRN"
     gene            14272..14784
                     /locus_tag="PLF48_01375"
     CDS             14272..14784
                     /locus_tag="PLF48_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNF48000.1"
                     /translation="MAHIISCRGFEPQLGSNCYVAPNATIVGDVLMGDDCSVWFNAVI
                     RGDVNSIRMGNKVNVQDNAVIHCTFEKTKTIIGNNVSIAHSAIVHGCTLEDNVMIGMG
                     AIVMDNCYIEKNALIAAGSVVLEGTRVEAGSIYAGVPAKKVKQLSEEVFKDQNERIAN
                     NYVKYAGWFK"
     gene            15032..16081
                     /locus_tag="PLF48_01380"
     CDS             15032..16081
                     /locus_tag="PLF48_01380"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNF48001.1"
                     /translation="MKTNIKLLAEICEVAGAPGFEQRVRELVVKNLEGHVDEIQFDNL
                     GSIYAVKKGKDASKRVMIAAHMDEIGFIVKYIDDNGFLRFHTLGGFDPKTLTAQRVLV
                     HGKKDLIGVMGTKPIHIMTPEERAKPVQTSEYFIDLGLPKEEVVKYVSVGDAITRERH
                     LIEMGNCVNCKSIDNRVAVYILIETLKMLKDFPYDVYGVFTVQEEVGIRGANVAAHNI
                     NPDFGFGLDTTIAYDLPGAKPEEQVTKLGEGTAIKIMDASTICDYRMVQYQKEIAAKY
                     KIKWQPEILPMGGTDTAGIQRMGKTGAIAGAISVPTRHLHQVIEMAHKEDIEASVLLL
                     KHCLEDIDQYDWSLK"
     gene            16231..16911
                     /locus_tag="PLF48_01385"
     CDS             16231..16911
                     /locus_tag="PLF48_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF48002.1"
                     /translation="MTPTDIGAFCAHCQKEVVDFTQHTPTEIASFVSNTKGKICGRLS
                     SAQLNEVYEYYIPVQKHNIKYAAGLALGLLATSKSIAQENKQAVEIITKIQDSKNNLQ
                     ITNIKGANEKITVHGKVIDGKTGEPLIGVNVEEENTANGAVTDIDGNYSIKLMKFPSK
                     LLFKYIGYADESINIGYKSKDSLMVKITPLEPISMGAVVVVKFNDDVYRGVKPIINHT
                     KRKHPKNK"
     gene            17058..>18351
                     /locus_tag="PLF48_01390"
     CDS             17058..>18351
                     /locus_tag="PLF48_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNF48003.1"
                     /translation="MIFDLDMIKKVYAEFPAKVDKAKALLNRPLTLAEKILFAHLHES
                     QNLEDFQRGKSYVDFAPDRVAMQDATAQMALLQFMQAGRKKVAVPSTVHADHLIQAKI
                     GADKDLQEAINKNNEVYNFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADAVDVMAGMAWELKWPKLIGVKLTGKLSGWTSPKDVILK
                     VAGILTVKGGTGAIVEYFGEGVTSMSCTGKGTIANMGAEIGATTSTFGYDESMSRYLQ
                     GTGRAEVAALADGIKDYLNADAECYAEPEKYFDQVIEIDLNTLEPHLNGPFTPDLATP
                     VSKMKDEALKNGWPLDVKVGLIGSCTNSSYEDISRAASLAKQVKEKGLKAKAQFTITP
                     GSEQVRFTIQRDGFIELFDEIGANVFANACGPCIGQWSR"
BASE COUNT         5736 a         3322 c         3352 g         5941 t
ORIGIN      
        1 gtattctaca ggctgggcat tgtctgcatt tacttttaca attttacccg atacttcact
       61 ttgtatctca ttaaataatt tcatggcttc aataatacat aagacatcgc cttttttaat
      121 cacatcgcct actttcacaa atgcaggcat atcaggagat gaggagcggt aaaaagtgcc
      181 aatcatcgga gctttgatgg ctatagccga tgagctttca gcagttgtat tttcttgttt
      241 tacttcattg gatggagctg gcaacgactg gagctgggca acaggtgcgg atactgatgc
      301 accactaact actgtttgct tgataaaatc acggtgcttg atgctgattt ttaagtcgcc
      361 ttcttccacc tttaattcac ccacattgtt gtcattcact aactgaatga gttttttgat
      421 ttgtgctata tccatatctc aaagatagca gaaatgaagg aattagaaaa tacttaaatt
      481 taagtgccaa atgtatttca tctcaaaaaa ttcaatttct ataaaaacat taggattatt
      541 ctattttaaa aagtggaggt gcaattacat cttcaacttt gtaaagcagt ctttttattt
      601 catcgtattc cctatatttt ttaagtgatg ggtgcagttt gttaaaatct tcaatgagca
      661 tttgcctatg tttaatataa tgaagcgcat ctaaacacat tctatgtaag acaatatacg
      721 atttgtaatg caatcttttg aatataattt tatctaactc aatttcaaga ctgtcaatta
      781 cacttaatat agaatcggaa attatcatat tagggttttt aggtaataga ttttcgattg
      841 gtcgctcaac agttgttcta aaactatctt gtataatcaa tgaaatatta tacgcatcat
      901 ttaactttga gtttgggata tcaacagtta atttattttt ctcaattatt acggatgcat
      961 ttcttaaatc taagaaacat cgttttatat gacgataatc gtcattggca cagaaaatca
     1021 ttttaccggg ttcttcgatg tttacactaa acttgccgga tttatttaca cccaaagtat
     1081 cttcaaatac aatcgtatca tttacctgaa aataaaaatg gtctaaagta tcataattta
     1141 agagcttata atggataaca tattcctttg aaaatgtcgt aacaaaaaaa gtaaccataa
     1201 aaaaaagaag taatccatgt ctttgaattt tgtcaaaatg aatttgccgc atcttaaaaa
     1261 tttataaatt gcatttacaa ataaatcata tacctgcaag ttttactttt tgcatgtaat
     1321 ataaatataa gtaaaaatgt ttaaaaacag aatattgttc tgattattta tttcttctgt
     1381 atttctttat ggcagcttaa gaattgtggt attttatata ctaccctccg cagcccagca
     1441 agcgattcgg attctttttt accaattcgt ctaactccgg catcaaagca acaggaaatt
     1501 ttactggatc aactagttct gttataagtg tgttttcttg aactatttta tttttcgttc
     1561 cagctttctt atacttctta gtgggcttca tcttatccga aatatacagc atatttttct
     1621 atttcttacc tttgctcacc aatgaaatgg atagctaagt ataaaggatt cttgaggcgg
     1681 gtgcgctttc tgcaatatat ttataatctc tttcataaaa aaggactgga acacaacaag
     1741 catttctaca atctgtatca aattaaaaag tccatttacg actctgtctc acaccgcgat
     1801 ttcaaaaatc tgcgacttaa atctccctgg atggacagaa tagttgggca aaatgaaatt
     1861 cttaatcatc ccgaatatat tcgttttccg gaagctgtag aagaacaatt actgcactgg
     1921 cacaaaacag gatttctcat ttgggaaaac ttcctcgatg aagaaaccat caactccatc
     1981 aactttgaac tggacgaact gattggtaaa aacaaggtgc aattcaatta cacatcacgc
     2041 aaaattttca acgcttacaa acattcttac actatccgca aggtcatcaa agacaagcgc
     2101 ttactgcaat tgtttcattt tatcatggaa cgaaatgcca tccctttcca gactatcaat
     2161 ttcatgaaag gaagcgagca gaaagcacat tctgattcta tacacatgag tacttttcct
     2221 gaaggaaatt tggttgcggc atggtttgcg ctggaagata ttggcgatat gcaaggtcct
     2281 gttttttatt atccgggctc tcaggatttg ccttatattt ataatgaaga ttaccctaac
     2341 gataatacga ggtttctgct ggatgaaaat gcgaatgaaa aatatgaggc aaaagtacaa
     2401 gaagtgctaa ataatcatcc tgaattaaag aaagaaattt atatgccaaa aaaaggcgat
     2461 ttgctcatct ggcatgctaa tttgttgcat ggtgcagaac ctataaaaga ctataactta
     2521 accagaaaaa gtatggtggt gcattatttt ggagaagatg tggtttgcta tcacgaaatc
     2581 agtgagcgac ctactatatt tgatacagaa ctggtgggcg aagtaaaaga aaaattcttc
     2641 aaaggagatt cggatgtctt tgatattggt gagtaactac cacaattcac tttcatcacc
     2701 ttcatcaggc ttgaagtctt taaataattt tcgggtttct attttttgcc gctgtcgttc
     2761 tatgattaaa tcggcaataa tctcagaagc attttcgccc ttatgctctg ccatcagtct
     2821 gcgcacttta tcttcatgca catcaatacg atataacata aacagcaaat gctcgaaatc
     2881 attgtttatc caatagttga ttttttgaat gatatatttt ctgaactctt caaaagaaga
     2941 aaaactcaat ccggtttctt ctatgagctg taaggtatat tcttgtatct tgttttcgtt
     3001 ttccatattt tccagctaaa agtaaagtta gtttattttc aggatttttg ttttatataa
     3061 ttttaaaaaa atcaaaatta atatttcaca ataagcgcag catatttacc catgcaaatc
     3121 ctgtcatgtt catgaacgac ttcgccaatc agcaaacgta tattttctcc cgttgcaagc
     3181 ttaggtattt tctttttttt gcgtgaaaaa ttgagataaa ttttcatccg ttctttttca
     3241 tgggttcttt cgtacaccag aattgattta tccaaaatat ccaaccggga catatttcct
     3301 agtaaaagtg cattttcatt tttacgcaaa aacaacagtt ctttataaac tgataataac
     3361 gaatttttgt cggtttgcat acttgctaca ttgcgttccg tataatttgc attcacaggc
     3421 agccatatct tttctgcact cgaaaaaccc gcatgctttc cattattcca ttgcatgggt
     3481 gttctgcaat tatctctgtt tatagccaca ggtagtttat ttactagaaa ctgcggtatc
     3541 catttgtatg gtttagccaa actgtctttt gcattgcgaa tagcaatatc gccattttgc
     3601 atcccgattt cttcaccata ataaacgaca ggaattgcac gaatagtcat ttgcaagacg
     3661 gctaacaact ttgctttttc caaatcatta ttcagtcgtc cctggctgcg ccattggtca
     3721 tgattgctga atacgacagt tggaatttta ggatgcggaa aattttgttc gtactgctgt
     3781 aatatgtttt taaagaacga tgccttaaac ttaaaaaaca aaatatcaaa cgagaaaata
     3841 agatgtaagc catcattatt gccaagatat cggctaattg tttcttgagt accaaaaacc
     3901 tcgcccagaa gaattcttgg tggtgaaaat tcatcagcta ccttgcgcag ttcttttgcc
     3961 agctcaaagt tttccggttg atttactgaa tactttctca actgaaaatt gcccccggga
     4021 tattcttcac tgggaatcat atgaaacaat gaaaacggat tatccctaaa ctccttgtcc
     4081 ttgataatac aattaaaaat atcgagccgg aaaccatcta ctccttttga caaccaaaac
     4141 cgacaaactt cccacatttg ttgttttact tccttgttat gataatttaa atcaggctga
     4201 aagggcaaga atgaagcata ataatattgc tttctgctat cacaataatg ccatccgctc
     4261 gggccgataa tactcttcca gttattcggt ttatctcgcc agatatacca gtcagcttta
     4321 gtattatcac gcgatgatgc cgattcttta aaccagacat gttcgtcaga agtatgattg
     4381 agcaccatat caaacataac cttcattcct ctttgatgta cttcatcaat caattgttct
     4441 gcatcccgca tagttccgta tacgggagct atggaaaaat agtcggaaat atcatagccg
     4501 aaatctcttt gtggagaagc aaaaaatgga gaaatccaga tggtttcaaa acccaaatct
     4561 gaaatataat ccagcttttg gataatgcct tgtatatcac ccatgccatc tccatttgca
     4621 tcataaaatg aacgcggata aatctgatag atagaatgag tgagcataca ctacaaaagt
     4681 gaataaaaat gcccgaactt taagaattta gcttattttt gtttgataaa atgaagaata
     4741 gttttgtata tattttcata gcgatatgta ttgtattcgc aggaatcagt tcgtgttcgg
     4801 taggtaataa agaaattccc aatcctacag gcaaccaaat agtagatacc acaggcatgt
     4861 caaccattaa ccttaatgtt tcaggagaca caagttattc gctgcatttg aaggtatatg
     4921 ctgatactgt agtcaatagt gtgctctttg tttccggaac agattacacc acacaagatt
     4981 cagtaacttt tgctacactt aaagatacgg tgggtgtcta catccctaat ttgcccagtg
     5041 gtttatttgt tttcagtaag aaacaaactt caggtcatgt cagatatccg cttactcagg
     5101 gcagtatttc cattacacaa agagatacca gtaccagaat ttatcaggga gtgataaatg
     5161 taaacaataa caatacttcc ggacagccgc attataccat cagtggtaat tttgttgtaa
     5221 aaaattagga ctcaacagct ttttggatat tttccaaaac agcatcccat gtataaaatt
     5281 cttctgccag tgtacgggca tttgtactga tagattccca ttgtgcttta ttatgtaaaa
     5341 gttcaataat cgcatctgcc tgttgccgtg cgttatctct gactatgata tctttaccat
     5401 ctgtaacttt taatccttcg acaccaatag tggtagtaac gcacggaatg ccgcgataca
     5461 agctgtttac tacttttact ttaatgccac ttccaaaccg caacggcgtg ataaataccc
     5521 tgcatttttg aaaataaggt tcaagatctt ctacaaaacc agtcaaaata atacgggtat
     5581 ctaatgcttg caatgcaagc aaacgcttgt cggcatgttt gccaataata taaagtttca
     5641 agtcgggctc ttgagttacc agtttgcccc atacttctct gtaaaaccac aacaaaccgt
     5701 caatattggc ttcccagctt aacgtgccaa tatacaacaa tgctttttcg gtctgtgcaa
     5761 attgcagatc aggcaaagct aaagtggcat catcaccaag atgataggtt tcaaaaaact
     5821 tacttcgatc agcacctatt ttcactagtt catctgtgtc attaggtgca gccaaaattg
     5881 catcggaacg attacaaatt tgcctttcat agtttttaat gcgccaggct tggtttaata
     5941 aagctaagcg tttgaaaaac cggggctcta catctgcata cctttgccaa atcagatatt
     6001 cgcaattgtg ttcgtgtaac actattttgc cacgaaaatc atcaggaata tattgaaaca
     6061 tgacatagtg atcgcaaaaa acaacatcaa aatcatgaat ccacgctttt atttttttag
     6121 caaatgatgc ggatgcatta cgatacaaat taagcggaat gccttttaga ttagagagga
     6181 ttaaattttt tgccgaacgg ggaatattta attcttccat gtaatcgttt tcgagattca
     6241 ggcttcgaag aaattcatcc cggtataagt tgtcatcatt tttcaaaaaa caggcaacgg
     6301 aaacggaaca gtgctctgct aaaaaacgta cggttttatt agatttaatc actccgccac
     6361 taacgggcgg atatggaagc gtaggcgtca gaaagagcag tttttttgtc attattaacg
     6421 tgaagataga gagtatctga caaaattaag catatcaaaa aataataaaa attattatta
     6481 ggtatctctg taatttccca ttaattttaa gccgtgttat tgccattaga atatcatttt
     6541 ccgggaaaag aaataaaaca gcatacctta gatcgctatc ttgcatccgg atattttcgc
     6601 accggaaatt atttactgcg aacccgggtg ttgtattatg ataaccagat actaaatacc
     6661 ttgcatattc gcatacgatt ggcaaaacac atattaccca aacggctgaa taaactcctg
     6721 caaaaaaaca atcggctctt tagctggaat atcaagccct tttgtattac cgaagaaaaa
     6781 gaaaagttgt atcaaatgca ccgcaaacga ttcagcagca acacttcgcc cagtttgtct
     6841 gcttatatgt ttgaccattt caacaaaaat ctttttgaaa cttacgagat aaatatttac
     6901 cggaacaatc aactgatcgg ctattctatc tttgacaaag ggcaggtaag catggccagt
     6961 attctgggta tttttcatcc tgactatgcg gcatacagca tcggcatgta cacgatgctg
     7021 ctcgaaattg aatttgcaaa aacagaaggc tataaatatt attatcccgg ctatgttgct
     7081 tatgaaccat ctaaattcaa ttataaattg cgcttgtctg atgtatttga ctattatgac
     7141 tggtatagta aacgctggat gagttttagt aaaaaagaca cacgcattaa ggtaaacgat
     7201 tactttgaac aacaattaaa tcatgccaaa gtatggctgg atgagttggg tattgcttat
     7261 gatgaatgtt tttatcccta tttttatatg ggaggcatgt accctcaatc agactgtgtg
     7321 aaaggtgtaa agcatttgtt aattcgcaat tttcagatca aaggcgttta ctatattctg
     7381 gaatttcatc ccgaaaagat gcaattgcta ttgagcggaa ttactgttca taaatatcaa
     7441 tttgaagatg atattgactt tgatgaggtg ctggaagaaa agaggtggaa gcgcgtatta
     7501 ttatacttac accccagtgt gaaaattact tcttctcaag atttgtatgc tgcttatttg
     7561 tttttgcaag agctctttac agcaaacata caacctccac caattccaaa cacttctttt
     7621 aaaaacccga accgcaaccg ctgattttct gttatttctt tttgttcaat atagactcat
     7681 acaattgaat atattcatct gtactgagtt ttttcactaa ctcgccgatg agagcgtatg
     7741 ggatatttcc ttctttctta aaacgcatac agcttttgcc catatctaat ttggctttgt
     7801 aatttttgtg atattcattt acgaaccagt ccaacagctc ttgtttcatg taaataccca
     7861 tgtgataata gttgacagag tttttttgca caccaatact cataaaaggc aatggcaatt
     7921 cgggcgtgca atggtaaccc tttgggtata agctatgagg cactacatag ccaatcatgc
     7981 catagttcat acattcttta aatcctttag gaagatgctt taaaatggtt gttctgagct
     8041 tttccgccat gattttttta tccggatcgt ctatctcgct caagtattgc tctacagttg
     8101 ctgctttaga tgtcattttg tttttttatt aaagataaac agaattgccg tttgttaaga
     8161 aaatgactga taaattaata ttgccagcct ttcggaattt gtatatttaa gcaatggatt
     8221 tgctacaggc cttaccttcg gctcagagag tgctgggaca ctataacatc aacttccttt
     8281 cggcgaaaga atggtcaact ttgatacagt tcggtcaatt gaaatcattc aaaaaaggag
     8341 aaaactttat tacggcaaat caaatcaata ctaagattgc atttatttta aaaggcggtt
     8401 tccgcttctt ttatttaaaa aatgaaacag aaattacttg tttgtttgct tttgaaaatg
     8461 atatgattgg cagttttgaa tgcaatatct taaaacagcc ttgcacacaa accattcaag
     8521 cattagaaga cagtgatgtg tttataattg attacaaaga tttggaaagt ttgtacgata
     8581 aaagccagaa atttgaaaaa gtaggcagaa ttatcttaga acattatatg gcatttttgc
     8641 aactgcgcat cacttcttat ttattagaca caccagaaga aagatatctt cgattaattc
     8701 acgaaacacc tgatttactc aatcgagtac ccttgcaaca tattgcatca tttatcggcg
     8761 ttacaccagt ttcgcttagt cgtattcgca agcgtatatt aaagaaataa ttgtatagcc
     8821 gtttcttctt tttggcgcat taaagcagcg tcctttttgg ttttatcttt ataaccgcat
     8881 aaatgcaata agccatgagc caacacacgt tgtaattcta cttcatagct cactttatat
     8941 tttcgggcat tatcctgtac tctttccaaa gaaataaaaa tatctccttc tatgcttttt
     9001 ggctcgctgc tgttgtcaaa cgtgataata tctgtatagg tatcgtgttg cagatactgt
     9061 atattcatgg ctaataactc ttcatccgtc aagaatacat aagtcagttc ttttatcttg
     9121 tacgcttcca cttcagcaat acgcttcaat gcctttttcc attgcacggg tttcgcaaac
     9181 gccggttttt tcacggcatc aaaataaaaa tgtatagcca tcattcaaaa ataacaaaaa
     9241 gcaatgagca actttattta ttcagataat ataaaatcaa acggttcact tcttccgctt
     9301 ttatgatatt catttcgtgt gtgccgccaa ataccggaat gggatttttt atgtttttgt
     9361 atggaaacat cgtatcctta gtgccatgta aatgaataat gtcttgacga taattttcat
     9421 ctccattcca ttttacaatg gcattaaccg cccagtacaa aaagctgggg tcattatttt
     9481 ttgccatact tatcgcaagc tcccttagtc cgggtgtttg cataatttct ttgataaatt
     9541 tagaacccac ttcaatactc ttaataaatc ctttgccggg caacaatttg tgaaaatgtg
     9601 taatctttaa ttgtttcagg ttaaagggca tttctttacg gctttttaca gagctgatta
     9661 ggattacttt ttcaggatga atgtgtttga tgatttccat ggtaataatg ccacccattg
     9721 aatgaccgat taaattaaaa ggctcgccgg tattgattaa tggaataaag cgtcgggcat
     9781 aatccgacat tttatctttt ttctcgtgtt ttggaaatgg aagataaaca gcattgggaa
     9841 tacagtcgta ttgcaggtga aatgctcgtt cgtctgtggc tattcccgaa ataaaatagt
     9901 ttctgattcc ttgcatttta tacttttttt acccacatgt tgccggcggc agaatggctg
     9961 atggtaatcg tcttgcggtc attgacaacg atagttacag aatcgtcaaa ttcttctttt
    10021 tcaataattc ttatcttgtc gttgagctga atttttatct tgttcaaata aaccagaaat
    10081 gcagttgatg catcatttac acctgccaat atatagtttt cttttagttt ggcatcggta
    10141 agtgctatct gttgggcaaa atgaaaataa ccgtgcttgt cgggaatagg atcgccatgt
    10201 gggtcaaatt tcgggaagcc taaaaactca tccagccgtt caactaattc atcagactgt
    10261 acatgctcca attgctcggc aatttcatga actttatccc atgtaaattt taaatttctc
    10321 accaagaagg tttcccatag tctgtgcttg cgcaatacct ttattgctgt ttgtctgccg
    10381 accttagtca atgaagcacc atagtatttc ttatactgca ctaatttttt gtcagacaac
    10441 tttttcagca tgtcggtaac tgttgccgga ctatgttcca gtttatatgc caggtcattg
    10501 gttttcacag tttcggtgtc cagatcatcc agtaactgga aaatatattt caggtaattt
    10561 tcttcaacag cagtaagcat ttgaaataaa tttttaggct aatctaaaaa aaatattgat
    10621 atactaaaag ataaaaatct attttaccaa aattatggca gcaaatgaca aaaaatggtt
    10681 agtgccggat agtgattttt cgctacccga atcatatgct actattgctg ttccaaaaaa
    10741 atcaggcttt ttcaggaaat tactggcatt caccggtccg ggtttaatgg tagccgtggg
    10801 ctatatggat ccgggcaact gggccacaga tattgaagga ggctctatgt ttggctatac
    10861 tttactttca gtcatattaa tctccaattt gtttgccatt ttacttcagt atttatctct
    10921 gaaattaggc atttccaccg gcaaggattt ggcgcaagca tgtcgtgctg catatagtcc
    10981 caaagtatct ttcgctttat ggatagctgc agaaattgcc attacggcta ccgatttagc
    11041 cgaggtaatc ggctcagcca ttgccataca attattattt ggcataccct tacccattgg
    11101 tgtgttaatt actgctgtag atgttttatt gattttgttt ttacaacaaa aaggatttcg
    11161 ttatttggaa attatcgtgg gtggactgat tggtattatt ttactttgtt tcctgacaga
    11221 attattatta tcccgacctc atattaatga aatagttcat ggactaatac cctcaacagc
    11281 cataattact aatcaggata tgctgtatat tgccataggt atcttgggag ctaccgttat
    11341 gccacacaat ttatatctgc atagcagtat tgtgcaaacg cgtcaatatg aaagaaatga
    11401 tgagggtaag aaagaggcca tccgatttgc tacaattgat tccacattat ccttgatgtt
    11461 ggcatttttt atcaatgcat caattttaat tttagctgct tcggtttttc atttcaatca
    11521 acatacagat gttgcagata tctatgatgc ccataagctg ttagaacctg ttttgggtaa
    11581 tcggcttgca tcggtattgt ttgcagtagc cttattagcc tccgggcaaa attcaacttt
    11641 aaccggaact ttatctgggc aaattgtcat ggaaggattt gttaacttac gactaagacc
    11701 ctggttacgt cgcttaatta ctcgcttact ggctatcatt ccggctatgg gggtagctgt
    11761 ttattatggc gaaagtggta ccggaaaatt gttggtattc agtcaggtgg ttttatctat
    11821 gcagttgagt tttgccgttg tgcctttaat ccggtttaca ggaaatacaa ccttgatggg
    11881 aaaatttgtc aacaaaccat tgaccaagat tttagcttgg attatagctc ttattattat
    11941 ctcactaaat gcctttttac tcgtcaattt ctttttcacc tgagtttggg ccaagtgttt
    12001 taaattaaaa atagttgatt ttttttatta gaaaccacct ccttttaatt cctcctcctt
    12061 gtagttggag gaaaaatgag aaatcaacta ttttttatcc ttatttttta cttaacccga
    12121 actcaggctt tttccgttaa attaacttta cgacaaactt acaagcctaa ctgtttcaga
    12181 tagtattcga gttgtccttt ttcaagagga gtttcaagaa aggcgtttac tttcttactt
    12241 gcattcacga ctttcgtagc cgggatacta ccttgccatc gcttatcaat ataaggcaac
    12301 caggagaaat cgctgctttc attcagcaga attacaggca ctgtgatttt ttgtttttta
    12361 acgtatttct ggagtttggt tatcgcacct ccaccatcta agctgacaaa aataaattgg
    12421 attttttcag cttgatgttc ttcaataaat tttttgagta caggcatttc ttccatacaa
    12481 ggacgacacc atgtagcaaa aaaattgacg atatacgtag tatcattatt gcgattgtat
    12541 agtgctttca gtccttcgat tttttgcaca ctgatttcct gagcacttgc acaataggca
    12601 atagtggata gtataaaaat tgtaaacagt tttttcatac caaacaagat acaaaaagtc
    12661 tgccaaacgc tacttgtttc aatacgttaa ctcctttacc tttgcaccat gaatgaggaa
    12721 atcttagata tgccactaat cgagaagccg gaaaaacgaa ttaccgcttt tgactgggca
    12781 gtatttatcg gactttccac catttggggc ttttctttct tttttattaa aaaagggctt
    12841 gaagtttttg aacctgtaca ggtagcggca tttcgcatga cgattgcatt cttatcctta
    12901 acacccatgt ttttcgtgca tgccaaacag ctcaaattac aggctcacca atggaaaatg
    12961 gttccacttt tgggcttatt tggtaatatg attcccgcca tttgcttttg tgtggccgga
    13021 acaaaaatag acagtgcact ttcgggcatt atgaattcta caacacctct ttttgcctta
    13081 ttaattggtg gtttgttttt tggagtgatg cttacacgaa acaaaatttt aggtgtgttg
    13141 cttggctttg tcggtgcaat tattattgtt ttatccaaac aaaaaggcat acattccatc
    13201 aattggtacg tattgctccc gttgttggca actatttgtt acgggttaaa tgccaatatt
    13261 ttcaaagcag gatttcagca aaataatccg gtagtgattg ccttgtggca atatgcattt
    13321 gttgcatttt tttcatttat ttatttatgg tattccgggg catttcatct attatttaca
    13381 aatttcggaa aggcctggca ctcgctaaaa tatttattgt tgctcggcat atttggaaca
    13441 ggcattgcac aagtgttttt taatgtattg gctcagcgta cctccgcctt gtttgccacg
    13501 atgaccactt atttgattcc tatggtagcc atactggttg gtttatttat cggagagaag
    13561 atacatattc ttcatctcat cggactggca attatcttat ccggtattta tattggctca
    13621 agaaattaac taagcaacct gagttttgac taagaaaaat aaggtgaaaa aaagttgatt
    13681 actcaatttc acccccaata ggtctaaaat tagaagcttt tgattgtaaa ataaaattag
    13741 gttcaaatta attcgattaa tatttagaca aataaatact aaatgtatat atccgaaccg
    13801 caaaagaatt aggtttgtga aagacacagc aatagaagta aaatcaaaaa tcagtagggc
    13861 tatagtgatt gctttttgat tttagcttcg gttttatccg ccgattggcg gataaaaatt
    13921 gccttgtctt tcacaaatgc tttttggtta ctttttggca tcaaaaagta acagcataat
    13981 ttttaactga atattttaga atttaactct attgttaaaa ttgaaatgct tataccgaac
    14041 tcaggttaaa tcaaacaaac taaaccaata agaatgacat taaacaaaac aacgatagtt
    14101 taatggcgtt tattggtgcc aaaagttggg cagttgcact tctttgcttt tgctgttgca
    14161 caagattgca tgctagtcaa aataacaata cttactacaa gaatataact tatttttttc
    14221 agggagaatt gacttttcat tgcttaaatt tacacaaaat aacgaaaaaa tatggcgcat
    14281 attatatcat gcagaggatt tgaaccccaa ttgggcagta attgttatgt ggcacctaat
    14341 gctactattg ttggcgatgt gctaatgggc gatgattgca gtgtctggtt taatgccgtg
    14401 ataaggggtg atgtcaactc gatacgcatg ggtaataaag tgaatgtgca ggataatgca
    14461 gttatacatt gcacctttga aaaaacaaaa acaattatcg ggaataatgt ttctatcgcg
    14521 catagtgcta tcgttcatgg ctgcaccctc gaagacaatg tgatgatagg catgggagct
    14581 atcgtgatgg ataattgcta catagaaaaa aatgcactaa ttgctgcagg ctcagttgtt
    14641 ctggaaggca cacgtgtgga agcaggcagt atttatgcag gcgtgccggc aaagaaagta
    14701 aaacaattga gtgaagaggt ttttaaagac caaaacgaac gcattgccaa caattatgta
    14761 aaatatgcgg ggtggtttaa ataaagtgat gagatataat aaaagaaata aaaaatgtta
    14821 ttttatgccc caaaaaattg catttaacac ttgaacttag aaaaaaagta acaagagttt
    14881 aatattaaca attaattgaa taagagggat gaattataaa tgctgaattt gaaccgcaca
    14941 acaggttgta gatatcatat cattttccac tctttaaatc ttaacaataa agcattaaat
    15001 aattccctac ttttacccgc tttttaaaaa aatgaaaaca aacataaaat tactggcaga
    15061 aatctgcgaa gtagcaggtg ctccgggttt tgaacaacgt gttcgcgaat tggtagttaa
    15121 aaatctggaa ggacatgtag atgaaatcca atttgataat ttgggcagta tctacgctgt
    15181 caaaaaagga aaggatgcat ctaaacgtgt gatgatagcg gctcacatgg atgaaattgg
    15241 atttatagta aaatatattg atgataacgg atttcttcgt tttcatacct taggcggctt
    15301 tgacccgaaa acattgaccg cacaacgtgt gttggtgcat ggaaaaaaag atttaatcgg
    15361 agtgatggga acgaagccta ttcatatcat gacgcctgaa gaaagagcta aaccggtgca
    15421 aacgtcagaa tattttatcg acttgggtct gcctaaagaa gaagtagtaa aatatgtatc
    15481 agtaggtgat gcaattaccc gtgaaagaca tctgattgag atgggaaatt gtgtaaactg
    15541 caaatccata gataatcgcg tagcggttta tatcttgata gaaacgctga aaatgctgaa
    15601 agattttcct tatgatgttt atggcgtttt taccgttcag gaagaagtag gcattcgcgg
    15661 tgcaaatgta gccgcacata atatcaatcc cgattttggt tttggcttgg ataccactat
    15721 tgcctatgat ttaccgggag cgaaacccga agaacaagta accaaactgg gcgaaggtac
    15781 agccataaaa ataatggatg cttccacaat ttgcgattac cgaatggtac aatatcaaaa
    15841 agaaattgcc gcaaaataca agataaaatg gcaacccgaa atattgccaa tgggtggcac
    15901 ggatacggca ggtatacagc gcatgggcaa aaccggagcg atagccggag ctatttctgt
    15961 acctacccga catttacatc aagttataga aatggcacac aaagaagaca tcgaagcatc
    16021 tgtgttgttg ctgaaacatt gtttagaaga tatagaccag tacgactggt ctttgaaata
    16081 ggaagatttt ctggtctgac acgattatcg tggcacactt aggctaaaaa aaaacgtcaa
    16141 agatatccag atttttagta tatttaagga gtgaagaaaa aaatatttca actccaaata
    16201 ccatagccat gccatgaagg ctggcaaaat atgactccaa cagatatagg ggcattttgc
    16261 gcacattgcc aaaaagaggt tgttgatttt actcaacata cgccaacaga aattgcatcg
    16321 tttgtttcaa atactaaagg taaaatatgc ggacgattga gcagtgctca attgaacgaa
    16381 gtttatgaat attatatacc cgtacagaag cataatatta aatatgcagc aggcttagct
    16441 ttgggtttgt tggctacttc taaaagcata gcacaagaaa ataagcaagc ggtagaaatt
    16501 ataactaaaa ttcaggattc aaagaataat ctgcaaataa caaatataaa aggtgctaac
    16561 gagaaaatta ccgtgcatgg taaagttata gatggaaaaa caggagaacc attgattgga
    16621 gttaacgttg aagaagaaaa tactgcgaat ggtgcagtta ctgatataga tggaaattac
    16681 tccataaagt taatgaaatt tccctcaaaa ttattattta aatatatcgg atatgccgat
    16741 gaatcaatta atataggtta caagagcaag gattcattaa tggtaaaaat aactccatta
    16801 gaaccaataa gtatgggagc agtagttgtt gttaaattta acgatgatgt ctatcgggga
    16861 gttaaaccaa ttataaatca cactaaaaga aaacatccta aaaacaaatg attttttatt
    16921 gtatagaatt agatgtgcaa gataattgtg ccacatcatc tcccgcaatt taattttact
    16981 taaacctttc ttacccaaaa aaagcgtacc tttgcgctcg aaataattca ttttttaact
    17041 tcataatttt atattccatg atttttgatt tggatatgat taaaaaggtg tacgctgagt
    17101 ttccggctaa agtggacaaa gcgaaagcac tcttaaaccg tcccttaact ttagccgaaa
    17161 aaatcctttt tgcacacttg cacgagtctc agaatttaga agatttccaa cgaggtaaat
    17221 cctatgtaga ttttgctccc gaccgtgtag ccatgcaaga tgctactgcc cagatggctt
    17281 tgttgcagtt tatgcaagcc ggacgcaaaa aagtggctgt tccttctacg gttcatgccg
    17341 accacttaat tcaggctaaa attggtgcag acaaagattt gcaagaggcc ataaataaaa
    17401 ataacgaagt atataatttt ttagcttcag tttctaacaa gtatggcatc ggtttctgga
    17461 agccgggtgc cggtatcatc catcaggtag tgctggaaaa ttacgcattt ccgggtggta
    17521 tgatgattgg taccgactcg cacacggtga acgcgggtgg tttgggtatg atagctatcg
    17581 gtgtgggcgg tgctgatgcc gtggatgtga tggcaggtat ggcatgggaa ctaaaatggc
    17641 ctaaattaat cggtgtaaaa ttaacaggca aattaagtgg atggacatct cctaaagatg
    17701 taattctgaa agtagccgga attttaaccg ttaaaggcgg taccggtgca attgtagaat
    17761 attttggcga aggcgtaaca tctatgtctt gtaccggtaa aggcacgatt gctaatatgg
    17821 gtgcggaaat tggtgctact acttctacat ttggttacga cgaatctatg tcaagatatt
    17881 tgcaaggtac ggggcgggct gaagtagctg cactggcaga tggcatcaaa gattatctta
    17941 atgcagatgc agaatgctat gcagagcctg agaaatattt tgatcaggta atagaaatag
    18001 acttaaacac cttagagcca cacttaaatg gtccgtttac gccggatttg gctactcctg
    18061 tttcaaaaat gaaagatgaa gccctaaaaa atggttggcc gcttgatgta aaagtgggtt
    18121 taattggttc atgtaccaac tcttcatatg aagatatttc gcgtgcagcc tctttagcca
    18181 aacaagtaaa agaaaaaggc ttgaaagcaa aagctcaatt taccattaca cccggctcag
    18241 agcaagttcg tttcaccatt cagcgcgacg ggtttattga gttgtttgac gaaatagggg
    18301 cgaatgtatt tgccaatgct tgtggtcctt gcattgggca gtggagcaga g
//
LOCUS       DAOQII010000014        17980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1468_length_17980_cov_6.229568, whole genome shotgun sequence.
ACCESSION   DAOQII010000014 DAOQII010000000
VERSION     DAOQII010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_17980_cov_6.229568"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLF48_01395"
     CDS             <1..741
                     /locus_tag="PLF48_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF48004.1"
                     /translation="KFTDGLPVSMGIEGRHGIRNAPTLLNSAYLNTTLWDGGVPNLEQ
                     QVLAPIENPNEMDFNLPEIVVRLNQIASYRELSMKAYGQLPSIYSITRAIACFERTLY
                     TGISKYDRYTYYNDPNALNDTEKRGMTLFFNERGDCFHCHGGYNFTDHSFQNNGLYEY
                     YADSGRARITLNDDDVGLFKVPSLRNVANTAPYMHDGSLATLEAVIEHYNSGGKYHRN
                     KSSILYQMHLTVQEKADLVAFLKALSDE"
     gene            863..1579
                     /locus_tag="PLF48_01400"
     CDS             863..1579
                     /locus_tag="PLF48_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF48005.1"
                     /translation="MSKKLSVIVPAYNEENTIQHILKKIAEVSLIENLQKEMIVVNDC
                     STDDTEMKVKEFQTQYPELEIRYFKHEINKGKGAALRTGFQNANGDFVIVQDADLEYD
                     PNEFNILVKPILDGFADVVYGSRFMGGRPHRILFFWHTIGNKLLTFISNMFTNLNLTD
                     METCYKMFRREIIRQIELKENRFGFEPEVTAKIAKIPKIRIYEVGISYYGRTYEEGKK
                     IGMKDAFRALYCILKYNFFS"
     gene            1595..2785
                     /locus_tag="PLF48_01405"
     CDS             1595..2785
                     /locus_tag="PLF48_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48006.1"
                     /translation="MHVKKWLGICLALFYSCTFCISAENVFSTNNILTEFGRKLNTNF
                     YEIIDYDALVPIQGLPKVITSKIYYEDSKEWDAEDSLLYDEKGRLVYLNNYPDEKSLI
                     YDGDKIIYISRKFSTEETYVYWFFYDAYDNPEIQIVAIKDNMTGKYTLHEVERHNTTY
                     FKDSVIVNVKSSRKYLTNSNGITKVFDRNGNIKYKSYINTRDVSVSINYIYKKFNNKY
                     VLQKEILEAAQGNIKSTATTAYTLANDGTVLKEELIQQDAFHLYFERYLVSTTFFKDS
                     SKKVIHDSYNTSGIQKSQHYKYDKYGNNTFNADLDYKDKNSYYISLYDYEYDSNNNWI
                     AKIEYMQETNKALEPLNEKMKLATYTRNIIYFTKDEKYTPEKAPDIPADLKKQITEQL
                     EKLK"
     gene            complement(2789..3307)
                     /locus_tag="PLF48_01410"
     CDS             complement(2789..3307)
                     /locus_tag="PLF48_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF48007.1"
                     /translation="MNEQLSRQYQGIQKTKTDLLNKIKTVDEEQLNRKPTDGSWSLGQ
                     VYYHCYLAEYGTIKTIEKNLKENKVKLQSEFADKIRNILLLIVLKSPLKFKAPAVVQK
                     VPEYITYNEIEELYADSTNDFNKILASLPASLIDKRIFKHPIAGLFNIEQTLNFVGEH
                     YRHHIMQIDRLL"
     gene            3374..4561
                     /locus_tag="PLF48_01415"
     CDS             3374..4561
                     /locus_tag="PLF48_01415"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF48008.1"
                     /translation="MFLDFFLLLKEEGLPVSIGEYLNLLEALNKRVTAYNIDDFYFLS
                     KTILVKHEQFLDRYDILFGQFFNGIKSDKLNEKEIPEDWLKKEMERLLSDEEKAMIEK
                     MGGLGKLMERLKQLMEEQKEKHQGGNKWIGTGGTSPFGHGGYNPEGIRIGGSGGQRSA
                     IKVWEKREFQNYAADVELNTRNIKLALKRLRTLTREGIDEELNLPVTIDKTCRNAGYL
                     DIAMQPSKKNRVKVLLFFDVGGSMDPYVEICEQLFSAAKTEFKHLEFFYFHNCLYESI
                     WKDNIMRFNDRIPTFEVLHKFNPDYRVIIVGDATMSPYELTAKGGSVEHYNDEEGAVW
                     VSRFVERYPNLVWLNPSNEYYWDGVPSIRILKEVVKDRMFPLTLDGLTKAMKTLKGKK
                     IVR"
     gene            4642..5499
                     /locus_tag="PLF48_01420"
     CDS             4642..5499
                     /locus_tag="PLF48_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNF48009.1"
                     /translation="MIHTFTGTENYIATDELQTAVNAAIALEKPLLIKGEPGTGKTML
                     AFEVAKALNKPLYTWHVKSTTTAQQGLYEYDAVARLRDSQFGDEQVNDIAHYIIKGKM
                     WQAFESDEQAVLLIDEIDKADIEFPNDLLLELDKMEFYCYELQKTIKAKTRPIVIITS
                     NNEKELPDAFLRRCFFHFIRFPERTTMIDIVNVHFPKLEEDLMEEALKVFYGLREIHG
                     IKKKPSTSELLDWIRLLKLGKNTKDHLAEIDYVKNSPPYFGSLIKNEQDFEHIARVKR
                     AGQNTFRKF"
     gene            5867..6319
                     /locus_tag="PLF48_01425"
     CDS             5867..6319
                     /locus_tag="PLF48_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48010.1"
                     /translation="MKKKLYSTLLCTLAIIGLLFNTHAKPTKKAISSPVIGSWKHYNS
                     AMLTKNNLGYKSRPELNYSKEEFTFYDNATFKHDFINEDGILIKSLKGKWSATNNKID
                     LVYDNLAYNLSTNYFFIGDELVIGQAFNHIILQKEDKQSQYAENNKSN"
     gene            6444..7028
                     /locus_tag="PLF48_01430"
     CDS             6444..7028
                     /locus_tag="PLF48_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48011.1"
                     /translation="MKTTANFITLFVFLLTSAVVLSGLLACNSSLQMTDEDFSELIRF
                     ENTIHEPGFGMQKNCISVLADKNMKVLTIAEVGKIKSNNEHLSSACDSYINKHAKLAS
                     RLMQYTDRMGVSVNWDTNEAEQVIADRMQNHQETFDEAVTFLMNESYKDFLHYFEKQK
                     NTLQQENIVKTVHQEIKEDANQQQEVYYASVVNQ"
     gene            7193..8281
                     /locus_tag="PLF48_01435"
     CDS             7193..8281
                     /locus_tag="PLF48_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNF48012.1"
                     /translation="MENQFASLGKVALVLFILLAVCLLLYCGATILMPLFFAVLLSIL
                     LNPAVNFLQAKNIPRTLAVFLVLIISLTIVICLCLFLFYQLTRFSEFMPQLKIQLDIA
                     IKKFLYWLSLKSNISYLVLVKKAGQLLTDTSKNLSGILGTTLSTVTSVLVLLFLIPIY
                     TFMFLFYKPLLLKFISSIFEVDKHERISNILSKSKLVIQNYLNGLIIESAIVSVLNSV
                     GLYIIGMKFAILFGIIGGLLNIIPYVGGMIAVVLMMTVALATLSAKAALWVLVWFIIV
                     QFIDNNVLMHYIVSSKVKINALASIIAVLAGGALCGVGGMFLAIPAVAFFKIIFDHID
                     TLKPYGILLGDDMPSQNISFFRSRKNDY"
     gene            8392..8550
                     /locus_tag="PLF48_01440"
     CDS             8392..8550
                     /locus_tag="PLF48_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48013.1"
                     /translation="MPITIIDTLAETAKSKPLEAYLDSLKRLFEASRLDAFFTTIYNP
                     ENYPDTKL"
     gene            complement(8618..9106)
                     /locus_tag="PLF48_01445"
     CDS             complement(8618..9106)
                     /locus_tag="PLF48_01445"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNF48014.1"
                     /translation="MKKHFLIFTRKLMATCTMLSIAMSSLTFAGETAKTSNSSTSKKP
                     LLIKVTNLVSPSATVVVGVYTPGNKFLDINDQYKVYYLKPKNGVLTAKLNDLTYGTYG
                     IALFQDLDGNGKINKNALGIPKEPYAFSNNFKPMMKAPNFNNCKFSYNAACNSIEIKL
                     LK"
     gene            9266..9826
                     /locus_tag="PLF48_01450"
     CDS             9266..9826
                     /locus_tag="PLF48_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNF48015.1"
                     /translation="MNIFVKFDLTYACKMILKDQLEKIGIEYQIISLNEVEFTQNLTD
                     QQREQLKDAFEHYGIEIIDNQKTALVQKIKDTIVEMIWKDDNMPNVTTSTYLADKLHH
                     SYGYLSNLFSEVTYTTIENFIIIQKIELAKQMLLSEKYTLTEISYELNYSSVAHLSNQ
                     FKKTTGLTPSSFQRIIKRRKELQQSK"
     gene            9833..10279
                     /locus_tag="PLF48_01455"
     CDS             9833..10279
                     /locus_tag="PLF48_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF48016.1"
                     /translation="MNTKPLSILLADDDEDDRLFFKDALEECRVKTMVETVNDGIELM
                     NLLLEESTVLPHILFLDLNMPRKNGIECLEDIRKSDRLKHITVAIYSTSGAERDIEDT
                     FVKGANVYIKKPNDFNALKEVLENVLSTKWQFHTSGLNKDTFLLNV"
     gene            10291..12090
                     /locus_tag="PLF48_01460"
     CDS             10291..12090
                     /locus_tag="PLF48_01460"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNF48017.1"
                     /translation="MKTLMSKKVSKIYSIVFISAIFILLFITALTYNQIKSMNDAQQL
                     IIHTYKVKLTLAQLSNNLRNVETNQRGYIITGDSSYLITYHIYKIKTDSLVTSLLALT
                     KDNKEQTEKLNKIRLLVKRRYEYLDETLILSNRAHYRSGISDSVKSNMLMGSNVMNLL
                     KDEETNFISTENQLLIDRENTSKTKRIIAPLFIFLIVLFSLGVFVAAYFRIDKDVRKL
                     TILNNDLRLKDISHSQAESVARLGNWQYDVNNDIFSVSDNYYSMIGAEKGEYDATIAN
                     YMISIHPDDLPYVEENAHRCLGGEEDFELRYRFYRKDTQELRHFRTVSRSVRNSNGIP
                     VTLGINQDITDSVMNIQMLEKQNQELLNANYELSSFNYVASHDLQEPLRKIQMFISRI
                     TGDKDNQLSDKSLDFLKKTQDSANRMQILINDLLQYSRISRSEHHFESIHLNQIIADA
                     WSNLAAMVEQKNAIIHADDLPDISGISFQISQLFFNLIGNSLKYTDGSRVPEIHISST
                     VVNGASIPFSSASADKNYLRISVLDNGIGFEQQYAQKIFTLFQRLHDKNTFSGTGIGL
                     AICKKIIDNHNGFIEAKGYPGKGAEFILYFPII"
     gene            12209..13102
                     /locus_tag="PLF48_01465"
     CDS             12209..13102
                     /locus_tag="PLF48_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNF48018.1"
                     /translation="MKSEIIKKLGKPFIVLKNAYNGFNDNNVIKLSASLAYFTLFSLP
                     PLLVILITITGSIFGKEAVRGELFFEIRNLVGAQAAIQIQEIVKNIELQQHTGLITAF
                     SFIILAFGASGIFSEIQSSLHLIWEIEPGQNKGFWHQVQNKLLSLSMIGSLSFLLMVS
                     LFINSILDIAGNYLFAYFKDSKVILLFIINNVIVFLVVSVLFLLIYKTLPRKRLRWKY
                     IYNASLFTSGLFMIGKYLISWYLSSSKLVSVYGAAGTLIILLIWVYYSAIILYFGAEL
                     AKANAIYNNDYETVRDKPQSK"
     gene            complement(13776..14186)
                     /locus_tag="PLF48_01470"
     CDS             complement(13776..14186)
                     /locus_tag="PLF48_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48019.1"
                     /translation="MATIKNQQDLQNAILTLEVQQQKEIDELSGQFKETIERINPANF
                     IKHTFKQINRTQEGQRNLIRTAVMLAVGYIAKRLIDRFMQKDRNHLVKFIGGGLQVAI
                     ATWVTKNGMRFQTLIFSVLSGLLKSYTGLFLPQT"
     gene            complement(14189..14533)
                     /locus_tag="PLF48_01475"
     CDS             complement(14189..14533)
                     /locus_tag="PLF48_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48020.1"
                     /translation="MPSSSSAFEQLAEQGQEYVKSSYELSKLKGINATANIITVLISK
                     LSIVLLVVLSLLMLSIAVALFFGKMLGNAGYGFLIVSVVYGILSVLAAYFLCDRIKTP
                     IANLIISHALND"
     gene            complement(14559..14870)
                     /locus_tag="PLF48_01480"
     CDS             complement(14559..14870)
                     /locus_tag="PLF48_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNF48021.1"
                     /translation="MNKRNLIIGVLAGVAAGAALGIMLAPDSGANTRKKILNKKDEYA
                     KSLKDEYKRLLKSVNSDLDDLQSDIDNIAAEITDDIEQKANQAKNAFNQTKSTITESL
                     S"
     gene            complement(14916..15071)
                     /locus_tag="PLF48_01485"
     CDS             complement(14916..15071)
                     /locus_tag="PLF48_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HNF48022.1"
                     /translation="MNNLLYIIAVILIIGWAVGFVGFHAGGLIHILLVIALIAVILRV
                     IQGKSPV"
     gene            complement(15120..15566)
                     /locus_tag="PLF48_01490"
     CDS             complement(15120..15566)
                     /locus_tag="PLF48_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48023.1"
                     /translation="MKYDKFYNETHRVVFLSFFILLFSSWMFLACKSKNEGAENKNTE
                     TTVTQTTTVETSPTQEWVRFKTDAEKKIQTYQSRIAELKAKIAKPGTPNLDKMRKNKI
                     NELEKKIVALRTSIENAETQTVTVSTETIKTMQVQLDSLETELNGF"
     gene            complement(15685..16593)
                     /locus_tag="PLF48_01495"
     CDS             complement(15685..16593)
                     /locus_tag="PLF48_01495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNF48024.1"
                     /translation="MNIIRLYPFLMLVAFISILFTGCATKNNKSATHTVQVNTPAKSE
                     ERIAATNLLQTEQLAGGVTYSKDTLKTKAALMAFKERYSTEQLQVIMALNRLDPNRLR
                     TNIQLIIPDTVLATFDSYSPFPNQLQAGQNIPKLFVVNQRIQLFAAYENGNLVRFGPV
                     STGKKATPTPNGLYHTNYKKKSKVSTVDGDWVMPWYTNIHNTEGIALHQYALPGYPAS
                     HSCVRLYEKDAMWVYNWADGWQLSDNGQKVLKNGTPVLIFGKFDFDQPASWKELPTNP
                     KIMELNAEEQQTLNEALNKIIGEQPS"
     gene            complement(16664..17554)
                     /locus_tag="PLF48_01500"
     CDS             complement(16664..17554)
                     /locus_tag="PLF48_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48025.1"
                     /translation="MKSKILKSGFAVAILAIALPTMAGEKNAVRVSEGFQHPESVVAH
                     RKSLFVSNTGAELKPLEKDGDGYITKLSRKNGEIVEEKFITDLDSPKGMFAKGKRLYV
                     ADVNKVYVFNAKTKKKIAEHDLSNQGAAYLNDVTKAVNGVYVSSTLENTLYKIKRNGK
                     VKEIKLKNANLNGINGVYRRPLGKVFVANFGHENEQNGNVGKFGVVRKKYKQIKKHGI
                     NDGIALKGGKVVYTDWVDKTKNAGNVITVKRINGKTTKVDLPVAIGGPSSIYADRRHR
                     ILWVPSMTDNKIVAIPFSKL"
BASE COUNT         5832 a         3113 c         3201 g         5834 t
ORIGIN      
        1 aagtttacag atggtttgcc ggtcagtatg ggtattgaag gacgacatgg tatacgcaat
       61 gcacctaccc tcttgaattc ggcttatctt aacactacac tttgggatgg tggcgtgcct
      121 aatttggaac agcaagtact ggcacccatt gaaaatccaa atgaaatgga tttcaatttg
      181 ccggaaattg tagtccgctt aaatcagata gcatcttata gggaactctc catgaaagca
      241 tatgggcaac tccccagtat ttacagcata acacgggcaa ttgcttgttt tgaacggacc
      301 ttgtataccg gcatatcaaa atatgacaga tatacctatt acaatgaccc taatgcactt
      361 aacgatactg aaaagcgggg aatgactttg ttttttaacg agcgtggtga ttgttttcat
      421 tgtcatggcg gttataattt taccgatcac agtttccaga ataacgggct gtatgaatat
      481 tatgccgact ctgggagagc cagaataaca ttaaatgatg atgatgttgg tttatttaaa
      541 gtgccttcac tgcgcaatgt tgccaacacg gctccatata tgcatgatgg ttctctggct
      601 acgcttgaag ccgttattga gcattacaac agtggcggaa aatatcacag aaacaagagt
      661 tctatcttat accagatgca tttgacagta caagagaagg cagatttggt cgcctttttg
      721 aaggcattgt cagatgaatg ataatgagtg aacaaattcc ggttcctcat acgtgtttat
      781 tttcagggga gggcgcgcct gcggcgcgcc ctcccttttt tatcgtttcc caaaataatt
      841 tgaatgctgt atatttgttc cgatgagcaa aaaactatcc gtcattgttc cggcttataa
      901 tgaagaaaat accattcagc atattttgaa aaaaattgcc gaggtttcgc ttattgaaaa
      961 tctgcaaaaa gagatgattg ttgtgaatga ttgttcaaca gatgataccg aaatgaaagt
     1021 caaggaattt cagacacaat atcccgaatt ggagattcgc tatttcaaac acgaaataaa
     1081 taaaggcaaa ggcgcagccc ttcgcaccgg attccaaaat gccaacggag attttgtcat
     1141 cgtgcaggat gcagacctcg aatatgaccc gaatgagttt aatattttag ttaagcccat
     1201 cctcgacgga tttgccgatg tggtgtacgg ctcaagattt atgggtggaa gacctcaccg
     1261 tattttattc ttttggcata ccataggcaa taaattattg acatttatct ctaacatgtt
     1321 taccaatctg aatttgacgg atatggaaac ctgctataag atgtttcgca gagaaattat
     1381 acggcaaata gaactaaagg aaaaccgttt tggctttgag cccgaagtaa cggcaaaaat
     1441 tgcaaagatt cctaaaatca ggatatatga agtaggcatt tcttattatg gcagaaccta
     1501 tgaagaaggt aaaaaaatcg gcatgaaaga tgcgtttcgt gcactttatt gtattttgaa
     1561 gtataatttt ttctcctaaa tttaacatcc acagatgcat gtaaaaaagt ggttaggaat
     1621 ttgtcttgct ctcttttatt cttgtacctt ttgcatatcc gctgaaaatg ttttttctac
     1681 aaataatata ctaacagaat ttggcagaaa actaaataca aacttttatg aaataataga
     1741 ttatgatgca ttagtgccaa tacaaggact gcctaaagta attacatcaa aaatatatta
     1801 tgaagattca aaagaatggg atgctgaaga ttcattactt tatgacgaga aaggtcgtct
     1861 ggtttattta aataattatc cggatgagaa atcattaatt tatgacgggg ataagataat
     1921 ttacataagt agaaaatttt caaccgaaga aacatatgta tattggttct tttatgatgc
     1981 ttacgataat cctgaaatac agattgttgc cataaaagat aatatgacag gaaaatacac
     2041 tttacacgaa gtagaaagac acaacacaac ttattttaaa gattctgtaa tcgtaaacgt
     2101 gaaatcttca agaaaatatc ttacaaattc aaatggaatt acaaaagttt ttgaccgaaa
     2161 cggaaacata aaatacaaat cttacattaa tacaagagat gtcagcgttt caattaatta
     2221 tatatacaag aaatttaaca ataaatatgt attacaaaaa gaaattttgg aagctgcgca
     2281 agggaatatt aaaagtacag caactacagc ctatacctta gcaaatgatg gaactgtttt
     2341 aaaagaagaa ttaattcaac aagatgcatt ccatctttat tttgaacgct atttggtaag
     2401 tactactttt tttaaagaca gctcaaaaaa agttattcat gacagttata atacttcggg
     2461 tattcaaaag tcacaacact ataaatatga caaatatgga aataacacct ttaatgctga
     2521 tctagattat aaggataaaa actcctacta tattagctta tacgactatg agtacgatag
     2581 caataataac tggatagcta aaatagaata catgcaagag actaataaag ccttagaacc
     2641 acttaatgag aagatgaaat tagcaaccta tacaagaaat attatttact ttacaaaaga
     2701 cgaaaaatat acgccggaaa aagcacctga tattccagct gacttaaaga aacaaataac
     2761 agaacaatta gaaaaactca aataacaact acagcaaacg gtctatctgc ataatgtgat
     2821 gccgatagtg ttctcctaca aaatttagtg tctgctcaat attaaacaat cctgctatag
     2881 ggtgtttaaa aatgcgcttg tcaatcagag atgcaggcag cgaagccaat attttattaa
     2941 aatcattcgt actgtctgcg tataattctt ctatttcgtt ataggtaatg tattcaggta
     3001 ctttttgaac aacggcaggt gctttaaatt ttaacggtga ttttagcaca attaataaca
     3061 aaatatttct gattttatct gcaaactcac tttgaagttt aaccttgttt tctttcaggt
     3121 ttttttcaat ggttttaatt gttccgtact ccgcaagata acaatgataa taaacctgcc
     3181 ccaaactcca gcttccatcg gtaggctttc gatttaattg ttcttcatca acagttttta
     3241 ttttgttcaa caaatctgtt tttgtttttt gtatcccttg atattgtcgt gatagttgct
     3301 cattcatttc taaatgtttg actaaaaata aaaaatagtt tctttatctc atttttcaat
     3361 ttacatttac agcatgtttc tcgatttctt tttgttgctc aaagaagaag gtttgccggt
     3421 tagcatcggt gaatatttga acttattaga agcattaaac aaaagagtta cagcatacaa
     3481 catcgacgat ttctattttt tgtcgaaaac aatattggtc aaacatgagc agtttttaga
     3541 ccggtacgat attttatttg gtcagttttt taatggaatt aaatcggata aactaaatga
     3601 gaaagaaata ccggaagact ggctgaaaaa agaaatggaa cgtctgctct ctgacgagga
     3661 aaaagcgatg atagaaaaaa tgggcgggtt gggcaagctg atggaacgcc tgaaacaact
     3721 gatggaagag caaaaagaaa aacatcaagg cggcaataag tggataggca ccggtggtac
     3781 ttctccattc ggacatggcg gctataaccc cgaaggcata cgcatcggag gcagtggtgg
     3841 gcaacgcagt gccattaaag tttgggagaa aagggaattt cagaattatg cagctgatgt
     3901 tgagctgaac acacgcaaca tcaaattagc tttgaaacga ttgcgtactc tcactcgcga
     3961 aggtattgac gaagaattaa atttgcccgt tacgatagat aagacttgtc gtaatgcagg
     4021 ctatttggat atagccatgc agccttcaaa aaagaacagg gtaaaagttt tattgttttt
     4081 tgatgtgggc ggctctatgg atccttacgt ggaaatttgc gagcaacttt tttctgcagc
     4141 aaaaacagaa tttaagcatc tggaattttt ctattttcac aactgccttt acgaaagcat
     4201 ctggaaagat aatatcatgc gattcaacga ccgtattccc acattcgaag tgctgcataa
     4261 attcaatcct gactatcgtg ttataattgt gggtgatgct actatgtcgc cctatgagct
     4321 gacagcaaaa gggggaagcg tagaacatta taacgatgaa gaaggtgccg tttgggtgag
     4381 ccgttttgta gagcgttatc caaatctggt ctggctcaat cccagcaatg aatattattg
     4441 ggatggcgta ccttctatac gaattctaaa agaagtagtg aaagaccgaa tgtttccgct
     4501 tactttagac ggactgacaa aagccatgaa aacattgaaa ggaaaaaaga tagtgaggta
     4561 atttgaggat aaggtaatta attatatttt aataataaaa taaaatttaa cgtattataa
     4621 ataactaata aaaaaacaac aatgatacat actttcaccg gaactgaaaa ttacattgct
     4681 accgacgaac tgcaaactgc agtgaatgcg gctatcgcgc tcgaaaaacc cctgctgata
     4741 aaaggcgaac ccggtaccgg gaaaacaatg ctggcttttg aagttgccaa agcactaaat
     4801 aaaccgctct atacctggca tgttaaatct accaccacgg cacaacaagg tttgtatgaa
     4861 tacgatgctg tggctcgttt gcgcgattcg caatttggcg atgagcaagt aaacgacatt
     4921 gcccattata ttataaaagg aaaaatgtgg caggcttttg aaagtgatga acaagcagta
     4981 ctgctgattg acgaaataga taaagcggat atcgaatttc caaatgattt attattggag
     5041 ttggataaaa tggagtttta ttgttacgaa ttgcaaaaaa ctatcaaagc caaaacacgc
     5101 cctatcgtta ttattacatc taataatgaa aaagaattac ccgatgcatt tttgcgcaga
     5161 tgtttctttc attttatccg gtttccggaa cgcaccacga tgattgatat cgtaaatgta
     5221 catttcccaa aactggaaga agatttgatg gaagaggcac tgaaagtctt ttatggcttg
     5281 cgcgaaattc acggaatcaa aaagaaaccc tctacctccg aactgttaga ctggatacgt
     5341 ttattgaaat tgggcaagaa cacaaaagat catttagccg agattgatta tgtaaaaaac
     5401 agtccgccct actttggctc acttataaaa aatgaacaag attttgagca tattgccaga
     5461 gtaaaacgag ccgggcaaaa tacgttcagg aagttttaag aataaaaaat tgttgtctta
     5521 tactaaataa tctattgttt ggattattcg taaaatgaaa tttaaatgta ttgttttaat
     5581 tatacatata ataaagcttt gcttatattt actttaactt gaagtctggt tttaattgct
     5641 tctctttgtt tttcaatggt gattataatt taggctgaat catttttttc agctatagat
     5701 atcttagttg tcatttagtt tgatattgtt ttatttcatc ctcacacatc aaacatagtg
     5761 cttaatattc ttaagcctca tctgtgattt ttataaaaat cagtaataat tctataagcg
     5821 gtctgttggg tatgtacgat aattttgccg tataaaataa ttgattatga aaaagaaatt
     5881 atattctact ctcttgtgca ctttggcaat tattggccta ttgtttaaca ctcatgcaaa
     5941 accaactaaa aaagcaatat catcgcctgt aatcggcagc tggaaacatt acaacagtgc
     6001 catgctgaca aaaaataatt taggttataa aagccgtccg gaattaaatt acagcaagga
     6061 agaatttaca ttctatgaca acgctacatt caaacatgat ttcatcaacg aagatggaat
     6121 tcttatcaaa tcattaaaag gaaaatggtc agcaacaaat aataagattg atttggtcta
     6181 tgataactta gcatataatc tttctacaaa ctacttcttt atcggagatg agttagtcat
     6241 tggtcaggct tttaatcata tcattttgca aaaagaagat aaacaaagtc aatacgctga
     6301 aaacaataaa agcaattgat ttgaatacca tcgtattatg taacattaga aaataatgat
     6361 gtaaggtaca aaatgaggaa tcacagcatc tttgtactat caaaagaaag aaaaataaat
     6421 taataatacc aaaacgaatc attatgaaaa caacggccaa cttcatcact ttattcgtgt
     6481 tcctgttaac ctcagcagtt gtactcagtg gattattagc ttgtaacagc agtttacaaa
     6541 tgactgacga agactttagt gaacttatac gatttgaaaa tactattcat gagcctggct
     6601 ttggaatgca aaaaaactgt atctctgtat tggcagataa aaacatgaaa gtgctcacca
     6661 ttgcagaagt gggtaaaatc aaatcaaaca atgaacattt aagttcagct tgtgacagct
     6721 atattaacaa acatgcaaag ctcgcctctc gattgatgca atataccgac agaatgggcg
     6781 tttcagtaaa ttgggatacc aatgaagcgg aacaagtaat tgccgacaga atgcaaaatc
     6841 atcaagaaac ttttgatgaa gcagtaacct tcttgatgaa tgaaagttac aaagacttct
     6901 tgcattattt tgaaaaacaa aaaaacacgt tgcaacaaga aaacatcgta aaaacagttc
     6961 atcaagaaat aaaagaagat gcaaaccaac aacaggaagt gtattatgca tctgtagtaa
     7021 accaataaaa ttataatgca gacaagcggt gcaagttaca caaagtaaat tttcaaaagt
     7081 cttcactcag gattcagttc cgtccccaaa cttcactctt acttcacgct gtctgcaatt
     7141 tttatatatc aaatcaacaa ccccgtacat agttgccaaa taattcaacc ttatggaaaa
     7201 tcaatttgct tcgcttggaa aagttgcctt agttctattt atacttctgg ctgtttgcct
     7261 cctcctatat tgcggggcaa ctattctgat gcccttgttt tttgcggtat tactatccat
     7321 attactcaat ccggccgtta attttttaca agcaaaaaat ataccccgta cgcttgctgt
     7381 ttttttagtc cttatcatct cactaacgat cgttatctgc ttgtgtcttt tcctttttta
     7441 ccaacttact agattcagtg agtttatgcc ccaattaaaa atacaactgg atatagccat
     7501 caaaaaattt ttgtactggc tttcactaaa atcaaacatc tcctatctgg tacttgtaaa
     7561 aaaggccggc caattactga cagatacttc taaaaactta tcggggatat taggcactac
     7621 cttatctacg gttacatcag tgctggtttt gttgttttta atcccgattt atacctttat
     7681 gtttttgttt tataaaccgc tattgttaaa gtttatcagc tccatttttg aagttgataa
     7741 acatgagcgt atcagtaata tattaagcaa atcaaaattg gtgattcaaa attatctcaa
     7801 tggattaata attgaatcag caatcgtatc tgtattgaac agtgtgggtt tgtatattat
     7861 cggaatgaaa tttgccatac tttttggtat aatcggcggt ttgctgaata tcataccata
     7921 cgtaggcgga atgattgccg ttgttttaat gatgacagta gcacttgcca ccttatctgc
     7981 taaggcggca ctatgggtgt tggtgtggtt tattattgtc cagtttattg acaataatgt
     8041 attaatgcat tatatcgtgt cgtcaaaagt aaaaataaat gcacttgcgt ccattattgc
     8101 agtactagca gggggagcat tatgcggagt aggtggtatg ttcttagcta ttcctgctgt
     8161 tgcttttttc aaaattattt tcgaccatat tgatacttta aaaccctatg gaattttatt
     8221 gggcgacgac atgccatctc agaacatctc attcttcagg agcaggaaaa atgattatta
     8281 agtaagcgca cacttataaa taaaaaagaa tcagaattaa accgaaagat tacaaaactg
     8341 gcacttgaaa tcaggaaaga atttccgaaa ttacataaca agacggagga aatgccaata
     8401 acaataattg atacgttggc tgaaaccgca aaatcaaagc ctctcgaagc ctatcttgat
     8461 tcattaaaaa gactatttga agcatcccgt ttggatgcct tttttacgac tatctataat
     8521 cctgaaaatt atccagatac caagctttaa aatttgcaat aaaaaaatgc accatagggg
     8581 cgcattaaaa ttggaaataa gaacgtgaat ttccggctta ttttaataat ttgatttcaa
     8641 tgctattgca tgcggcattg taactaaact tacaattatt aaaattaggt gctttcatca
     8701 tcggtttaaa attattggag aaagcatacg gctctttggg tattcccaat gcgtttttat
     8761 taatttttcc attgccgtcc aaatcctgaa acaaagcaat tccgtatgtt ccgtatgtaa
     8821 gatcgtttaa cttagcagtc aataccccat ttttaggttt cagatagtaa actttatatt
     8881 gatcattaat atctaaaaat ttgtttccgg gtgtgtaaac accaactact acagtagcag
     8941 atggcgaaac taaattggta acctttatca aaagtggttt tttggacgtt gaactgttgc
     9001 ttgtcttcgc cgtttctcct gcaaaggtaa gactgctcat agcaatagac aacatcgtac
     9061 atgttgccat taactttctg gtgaatatca aaaaatgttt tttcataaaa tgttatttta
     9121 atgcaaatat gttataactt cttcaacttt tatttcacta ttcatatcaa attttacaag
     9181 acttttatat ggcatattat tttatgcatt aattaatttc gtaacggatt gtcacttatt
     9241 ttccttaact ttaagtttta ttgccatgaa tatttttgta aaatttgacc tcacttatgc
     9301 ctgtaaaatg attctaaaag accaattgga gaaaattggc atagaatatc agataattag
     9361 cttgaatgaa gtagaattta ctcaaaatct tacagaccaa caacgggaac aattaaagga
     9421 tgcatttgaa cattatggca ttgaaataat tgacaatcaa aaaaccgcct tagtacaaaa
     9481 gataaaagat actattgtgg aaatgatatg gaaagacgat aatatgccta atgtcaccac
     9541 atccacttat cttgcagata agctacatca cagttatggc tatctgtcaa atttattctc
     9601 cgaagtaact tatacgacaa tagaaaactt tatcatcatt cagaaaattg aacttgcgaa
     9661 acaaatgctt ttaagcgaga aatatacact caccgaaatt tcttatgagc taaactacag
     9721 cagtgtggca catttatcca atcagttcaa aaaaacgaca ggcctcactc cttcgtcatt
     9781 tcagcgcata ataaaaagga gaaaagaatt acaacaatct aaataatata aaatgaatac
     9841 caaaccacta agcatattac tggccgatga cgatgaagac gatcggttgt tctttaaaga
     9901 tgctttagaa gagtgccggg ttaaaacaat ggttgaaaca gtgaatgatg gtatcgaatt
     9961 aatgaatctg cttttggaag aaagcacagt cttgccacat attctatttt tagacttgaa
    10021 catgcctcgt aaaaacggca ttgaatgctt agaagatata cgaaaatctg acaggctaaa
    10081 gcacattaca gttgccatct attctacctc cggagctgaa cgcgatatag aagatacttt
    10141 tgtaaaaggc gctaatgttt atatcaaaaa acccaatgat tttaatgcgc tgaaggaagt
    10201 actcgaaaac gtgctgagta ccaagtggca gtttcacacg tccggactaa ataaagatac
    10261 ttttttgctc aatgtataaa ttgatctgca atgaaaacac tgatgagtaa aaaagtttca
    10321 aaaatatatt ccatcgtgtt tatctcggca atcttcattt tattattcat tacggcactt
    10381 acttacaatc aaattaaatc aatgaatgat gcacagcagc tgatcattca tacatacaag
    10441 gtgaaactga ccttagctca actttccaat aatctaagaa atgtagaaac caaccaaagg
    10501 gggtatatta taaccggcga tagttcctat ttgattacct atcatattta caaaataaaa
    10561 acggatagcc ttgttacgtc tttgttagct ttaaccaaag acaataaaga gcagacggaa
    10621 aaattaaata agataaggtt gttggttaaa aggaggtatg agtatttaga cgaaacgctg
    10681 attttgagca accgagctca ttacagaagt ggaatatcag actctgttaa atctaatatg
    10741 ctgatgggat cgaatgtgat gaatctgctg aaagatgaag aaaccaattt tatcagcacc
    10801 gaaaatcagt tactgataga cagagagaat acgagcaaaa caaaaagaat tattgctcct
    10861 ttatttattt ttcttattgt attgttctca ctcggcgtat ttgtggctgc ttatttcaga
    10921 attgataaag atgttcgaaa gctgacgata ttaaataatg accttcggtt aaaggatatt
    10981 tcacacagcc aggctgaatc tgtagccaga cttggcaact ggcaatatga tgtgaataat
    11041 gatatctttt cggtttctga taattattat tcgatgatag gtgccgagaa gggtgaatat
    11101 gatgccacta ttgcaaatta catgatcagc atacatcctg atgatttgcc ttatgtagaa
    11161 gaaaatgcac acagatgcct gggtggcgaa gaagactttg agctgagata ccggttttat
    11221 cgcaaggata cgcaagagct gcgccatttt agaacggtaa gccgttcggt aagaaattcc
    11281 aatggcatac cagtcacctt gggcataaat caagatatta cagattcagt aatgaatata
    11341 caaatgctcg aaaaacaaaa ccaggagttg ttgaatgcca actatgaact ttcttctttt
    11401 aattatgtag ccagtcatga tttgcaagaa ccgctgcgta aaatacagat gtttatttca
    11461 cgcatcacag gagacaagga caatcagctt tcagacaagt cacttgattt tcttaaaaag
    11521 acacaagact cagccaacag gatgcaaatc ttaataaatg atttattgca atactccaga
    11581 atctccagga gcgaacacca ctttgagtcc atccatttaa accaaatcat agcagacgca
    11641 tggtctaatt tggctgcaat ggtcgaacaa aaaaatgcca ttattcatgc cgatgattta
    11701 ccggatattt ctggcatttc tttccaaatc agtcagttgt tttttaattt aatcggcaat
    11761 tcgctgaaat ataccgatgg aagccgagtg ccagaaattc atatcagttc tactgtagta
    11821 aatggtgcct ctattccgtt ttcatctgct tcagccgata aaaattattt gcggattagc
    11881 gttcttgata atggcatcgg ctttgagcaa caatatgcac agaagatatt tacattgttc
    11941 caaagacttc atgataaaaa tactttttca ggtaccggaa ttggtttggc tatctgcaaa
    12001 aagataattg acaaccacaa cggatttata gaagccaaag gatatcccgg aaaaggtgcg
    12061 gagtttatct tgtactttcc aataatataa tgagtcatct tttaaagata aaaacggcag
    12121 tatgattatt gtttttggtg tgtaaagctt ataacgaaca gctgaaattt tgtaagtaaa
    12181 agatgcttga tttattcaaa cttgcaacat gaaatctgaa attattaaaa agctgggtaa
    12241 gccatttatc gtgctgaaga atgcatataa tgggttcaat gataataatg taattaagct
    12301 cagtgcctct ttagcttatt ttactttgtt ttcattgcct ccgctattag ttattctgat
    12361 tactatcaca ggaagcattt ttggaaaaga ggcggtaaga ggtgaattgt tttttgaaat
    12421 cagaaatctg gtgggtgcac aagccgccat acaaattcag gaaatagtaa aaaatattga
    12481 actacagcaa cacaccggac ttataacggc gtttagtttt atcatattgg catttggtgc
    12541 ttcaggtata ttttctgaaa ttcaaagttc gctgcatctt atctgggaaa ttgagcccgg
    12601 acagaataaa ggattttggc accaagtaca aaataaactc ttatccctgt ctatgatagg
    12661 ctctttgtca ttcctgttaa tggtcagtct ttttataaat tctattttag acatagcagg
    12721 caattatctt tttgcctatt tcaaagactc gaaagtcatc ctattgttta tcataaacaa
    12781 tgtcattgta tttttggtgg tgagtgtatt gtttttgttg atttataaga cattaccccg
    12841 caagagatta agatggaaat acatttataa cgcttctctt tttacttcgg gtttgtttat
    12901 gataggcaaa tacctgataa gctggtatct gagtagttca aaactggtaa gtgtttatgg
    12961 tgctgccggt acgcttatca tcttgctgat ttgggtatat tattctgcca taatcctata
    13021 tttcggtgcc gaactggcaa aagcaaatgc aatttacaat aatgattatg aaaccgtaag
    13081 agataaacct caaagcaagt aattgtcttt tttttggcaa aaatgaattt attggcgtat
    13141 ttgctttctt tgcttctgtc caagtatata agctttcagc agcatattaa tttcctctct
    13201 cgaaatattg ctgatgcgtt tgatagaaac atctattcgt agtgtgagaa aatataaatt
    13261 cttaaaaagc gttgatttct tatgcattct tttgatctta tcctttattg aatattttta
    13321 tcatcactag tataaacgct atcagaagca atgcatgaat ggcatagcta tgtgtgttca
    13381 acacaactaa aattatccaa ctaataatca aaatcagtat caatatattt aataccattt
    13441 tcatatctga cagctttgag caaactctat ctttgcaatt cgttaaaaag aagacacgta
    13501 aatctgtgtt atcttgttaa ttcaaatacg aatttaataa ccctcctctg ttttggtgtt
    13561 acaacatgtt ttccatttct tacataattt ccatattact ccactatcct ttctttatta
    13621 tgaatacaac caagaatgct attattaaat gcaataaaaa aactgctccg actggggcag
    13681 tatatataaa atcttattct aaaacgcagg tattaacaat ttctttgagc atctaaacgc
    13741 tgattggttt tagctaggtt ctacgtctat atgttctaag tttgaggaag aaaaagccca
    13801 gtataagatt ttaaaagccc ggacagaaca gaaaagataa gtgtttggaa tctcatgcca
    13861 tttttagtaa cccaggtagc aatggcaact tgcagacctc cgccgataaa tttcactaaa
    13921 tgatttctgt ctttttgcat aaaacggtca atcagccgtt tggcaatata gcctacggct
    13981 aacattacgg ccgtccggat taggtttctt tgtccttctt gtgtcctgtt tatctgcttg
    14041 aaagtgtgct ttatgaaatt agctggattt atgcgctcta tggtttcttt aaactgtccg
    14101 cttaattcat ctatttcttt ttgctgctgc acctctagtg taaggattgc attttgcaga
    14161 tcttgttgat tttttatcgt agccatgatt agtcgtttaa tgcgtgagag ataataagat
    14221 ttgctatcgg agtcttaatt cgatcgcaca agaagtatgc agccagtaca cttagtatgc
    14281 cataaacgac cgatacgatt aagaaaccgt agcctgcatt tcccagcatc tttccgaaaa
    14341 acaaagcaac agcgatgctc agcataagca agctcaacac aaccaatagc acaatactca
    14401 gttttgaaat taatacggta atgatattgg ctgtcgcatt gatgcctttt agctttgaca
    14461 gttcatacga gcttttcacg tattcttgtc cttgttcggc aagttgctca aaagcagatg
    14521 atgaagatgg catactacgg gtttaatatt acagaagatt agctcaagct ttctgtgatg
    14581 gtagattttg tttgattgaa agcgttttta gcctgatttg ctttttgctc aatatcatca
    14641 gttatttcag ctgcgatatt atcaatatct gattgcaagt cgtccaaatc gctgtttact
    14701 gatttcaata aacgcttgta ttcatctttt aatgatttag cgtactcatc tttcttgttc
    14761 aagattttct tgcgcgtatt tgcgccactg tccggcgcca gcataattcc caaggcagct
    14821 cctgctgcta ctccggccaa tacaccgata attaaatttc ttttgttcat ggtcgtgttg
    14881 ttttgtttgt gattaataat ttagatgttg atttactaaa caggactttt gccttgtatt
    14941 actcttaaaa taacggctat taaagcaata acaagcaaga tgtgaatgag cccgccggca
    15001 tggaagccta caaagcctac agcccaacct ataataagta tgactgcaat gatgtacaat
    15061 aaattgttca tgataataaa ttttataagt taaaaattgt ttttaaaaaa ttatagagat
    15121 caaaatccgt tcagctctgt ttctaaactg tcaagttgta cttgcatggt tttgatggtc
    15181 tccgttgaaa ctgttaccgt ttgcgtttct gcattttcaa tggaagtgcg caaagcaact
    15241 attttctttt ccagttcatt aattttgttt ttgcgcattt tatccaaatt tggagtaccg
    15301 ggcttagcaa tcttagcttt cagttctgct atacgggatt gatacgtttg aatctttttt
    15361 tcggcgtctg ttttaaaacg cacccattcc tgagtggggc ttgtttcaac ggtggtggtt
    15421 tgtgtaaccg tagtttccgt gttcttgttt tcagctccct catttttaga tttacaagcc
    15481 agaaacatcc aggatgagaa taataagatg aagaatgaca aaaaaactac tctgtgggtt
    15541 tcgttataga atttatcgta tttcatgttg ttttttttac gcagttaaaa aaatttgtta
    15601 tgtcaaaatt gctctgtttt ttagtgctgt gtcttatatg aattttcgtt tattttacaa
    15661 gatcttcatg tataaagaaa tagattaaga tggttgttct ccaatgattt tatttaaagc
    15721 ttcattaagc gtttgctgtt cttcggcatt cagttccatt atctttggat ttgtcggtaa
    15781 ttctttccag gatgccggtt ggtcaaaatc aaatttccca aatatcaaaa ccggagttcc
    15841 gttttttaat accttctgac cattgtctga aagctgccat ccatctgccc agttatatac
    15901 ccacatagca tctttttcat acaaccttac acaggagtgg ctggccggat agcccggcaa
    15961 agcatattgg tgtaaggcaa tgccctctgt attgtggata ttcgtatacc aaggcattac
    16021 ccaatcacca tctacagtac taaccttaga ttttttctta taattagtat gatataatcc
    16081 gttgggagta ggagttgcct ttttgcccgt gcttacggga ccaaatctta ccaaattgcc
    16141 attttcataa gcagcaaata attgtatgcg ctggttaact acaaataatt ttggaatgtt
    16201 ttgccctgct tgcagttgat tgggaaacgg gctgtaagaa tcaaatgtag ccaatacggt
    16261 atccggaata ataagctgga tatttgttct aagacgatta ggatctaatc tgttcaaagc
    16321 catgatgact tggagctgct ccgtactgta tctctcttta aatgccatta acgctgcttt
    16381 tgttttcaac gtgtctttac tataagttac accgccggct aattgttctg tttgcaagag
    16441 attggttgcc gctattcttt cttcactttt agctggagta tttacttgca ccgtatgggt
    16501 agcactttta ttgtttttag tagcacatcc agtgaatagt atgctgataa atgcgacaag
    16561 catgagaaac ggatataggc gaataatatt cataatgttc agattgagat gattagaatt
    16621 taaatgatac agtacacgca gtcagctctg ctgattttgt gttttaaagc tttgagaatg
    16681 gaatggctac gattttattg tcagtcattg atggcaccca taaaattcgg tgtcttctgt
    16741 ctgcataaat acttgatggt ccgccaatag caaccggtaa atctacttta gtggttttac
    16801 catttattct tttaacggta atgacatttc ctgcgttttt tgttttgtca acccagtccg
    16861 tataaaccac ttttccacct tttaatgcaa taccgtcgtt aataccatgt tttttaattt
    16921 gtttgtattt tttgcgaacc acaccaaatt ttccaacatt accgttttgc tcattttcat
    16981 ggccaaaatt agcaacaaat acttttccca atggacgacg atacactcca tttattccat
    17041 ttaagttggc atttttgagt ttaatttctt ttaccttccc gttgcgtttg attttgtaca
    17101 atgtattttc cagtgttgaa gaaacataaa caccatttac agccttagtt acatcattaa
    17161 gataagctgc accctgatta gataaatcgt gttcggcaat tttctttttt gttttagcat
    17221 tgaacacata aactttgttt acatcagcta cgtaaagtcg ttttccttta gcaaacatac
    17281 ctttgggaga atccaaatcg gtgatgaatt tctcttctac aatctcacca tttttgcggc
    17341 tcaatttagt gatgtatccg tcaccgtctt tttccaatgg ttttaattct gctccggtgt
    17401 tagatacaaa taaactcttg cgatgtgcta ctacactttc cggatgttgg aagccttcgc
    17461 ttacgcgtac tgcatttttt tcacctgcca ttgtcggcaa tgcaatagca agtatagcta
    17521 ctgcaaatcc tgattttaaa atttttgatt tcatgattgt ttgtttttag tgtttaattg
    17581 atttgttatt gtttattttt tttgttcgtt tgatgaaaca aaattatcac cgtttattga
    17641 ctggcgtctt acacaattcc atactgatgt tgtataattt ttaacgaaat aaatgcatta
    17701 taaagagcct gttttaatat tttagtaaat actgtttgtc ttatatagcg taaatttatt
    17761 tatcataatc ttaatttttt atatatacta ctttgtgtat tattttaatg tagcttattt
    17821 tgaaataaaa aaatcccgca tacctgcggg atcgagaact aaaagcgtta tgatataata
    17881 acctgagttc gggttaagaa aattaaggtg aaaaaaatag ttgaattctc attttttcct
    17941 ccaacttaac aaggaggagg aattaaaagg aggtggttct
//
LOCUS       DAOQII010000015        17752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1503_length_17752_cov_5.929028, whole genome shotgun sequence.
ACCESSION   DAOQII010000015 DAOQII010000000
VERSION     DAOQII010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17752
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1503_length_17752_cov_5.929028"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="map"
                     /locus_tag="PLF48_01505"
     CDS             <1..618
                     /gene="map"
                     /locus_tag="PLF48_01505"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNF48026.1"
                     /translation="FPATLCVSPNEQVVHGIPSDREIKEGDIVSIDCGVCKDGYYGDS
                     AFTFPIADITPEIKRLLQITNNSLYLGIEKAVVGNRIGDVSFAVQKYTEIDNKYGVVR
                     ELIGHGVGKNLHEEPDVPNYGKRGKGPVMHEGLVIAIEPMINMGTYQVRQLSDKWTVV
                     TKDGKPSAHFEHTIAVKRGKADILSDHNLIYEAIKKSSYIKDFRD"
     gene            643..861
                     /gene="infA"
                     /locus_tag="PLF48_01510"
     CDS             643..861
                     /gene="infA"
                     /locus_tag="PLF48_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNF48027.1"
                     /translation="MGKTDLIKLDGTITEALSNAMFRVKLENGHEILATISGKMRMHY
                     IRILPGDKVAVEISPYDLSRGRITFRYK"
     gene            881..997
                     /gene="ykgO"
                     /locus_tag="PLF48_01515"
     CDS             881..997
                     /gene="ykgO"
                     /locus_tag="PLF48_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNF48028.1"
                     /translation="MKVRASVKKRSEDCVIVRRKGRVYVICKKNPKFKQRQG"
     gene            1000..1377
                     /gene="rpsM"
                     /locus_tag="PLF48_01520"
     CDS             1000..1377
                     /gene="rpsM"
                     /locus_tag="PLF48_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNF48029.1"
                     /translation="MARVSGIDLPKNKRGYIGLTYIYGIGRSSAREILAKAEVDPEKK
                     VHEWNDDEVTAIRNIINADFKVEGALRSEVQLNIKRLLDIGCYRGIRHRKGLPVRGQS
                     TKRNARTRKGKRKTVAGKKKVTK"
     gene            1404..1796
                     /gene="rpsK"
                     /locus_tag="PLF48_01525"
     CDS             1404..1796
                     /gene="rpsK"
                     /locus_tag="PLF48_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNF48030.1"
                     /translation="MATAQKKAAKKRVVKVEAEGYVYIKATFNNIIISYTNKAGQVIS
                     WSSAGKMGFRGSKKSTPYAAQLASQDAGTKAYEAGMRKAEVFVKGPGGGRESAIRSIG
                     NSGIEVTIIRDVTPLPHNGCRPPKRRRV"
     gene            1863..2471
                     /gene="rpsD"
                     /locus_tag="PLF48_01530"
     CDS             1863..2471
                     /gene="rpsD"
                     /locus_tag="PLF48_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNF48031.1"
                     /translation="MARYTGPRTKKSRAFGEPIFGYDKSYEKRKYPPGQHGNGRKRSS
                     QSEYAIQLKEKQKAKYTYGVLERQFYKTFEEASRKHGVKGENLLRLLEQRLDNTVYRL
                     GLAPTRSAARQLVTHKHITLNGRIVNIPSCTVKVGDVISVREKSKGLPLVQDTVATGR
                     VKKYPWLEFNTEKLEGKYVEIPNRDQIPETIKEQLIVELYSK"
     gene            2558..3553
                     /locus_tag="PLF48_01535"
     CDS             2558..3553
                     /locus_tag="PLF48_01535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNF48032.1"
                     /translation="MAILSFQKPDKILLQKATDFEGTFEFAPLEPGFGNTIGNALRRV
                     LLSSLEGYAITSVKIAGVNHEFSTIKGVIEDVTEVVLNLKQVRLKRVLQDSEAGFEKL
                     YISLKGKEQFLASDIESHTNVFVVTNPELVICNMEKNVNLEMEITIGKGRGYVSAEDH
                     YVDNSIVGLIPVDSIYTPIKNVRYKVENTRVEQRTDFEKLIIDVKTDGTIHPEDAVKE
                     AAKILIQHLMLVSDENITFNTEDKAQEEVVDEHILHMRKILKTPLEDLDLSVRAYNCL
                     KAAKINSLSELVQYDTNELLKFRNFGRKSLVEIEELIIEKGLHFGMDLSKFKLED"
     gene            3628..4152
                     /gene="rplQ"
                     /locus_tag="PLF48_01540"
     CDS             3628..4152
                     /gene="rplQ"
                     /locus_tag="PLF48_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNF48033.1"
                     /translation="MRHGNKINHLGRKYGHRSALLKNLAVALIVNKRIETTIAKAKEL
                     RGYIEPLITKAKENTTTNRRTVFSYLQDKTAVKELFDVISAKVGSRPGGYTRIVRLGF
                     RQGDAAEVAIIELVDFNEVYTPKSGATAEPKKKTRRAGGAKKKAETAEVVEEVKAEET
                     QTAPEATTEEKTEE"
     gene            4284..5126
                     /locus_tag="PLF48_01545"
     CDS             4284..5126
                     /locus_tag="PLF48_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNF48034.1"
                     /translation="MTKQNKLVAPFLKWVGGKRQLMSAIVQLLPKNIKELKYTEPFIG
                     GGAVFFYLQPQKAIINDCNQELINVYNVVKNQLEDLIIDLKKHENTSDYFYKIRGLDR
                     LNTFNKLPALQRASRVIYLNKTCFNGLYRVNNAGEFNAPFGRYKNPNIVNEPMLRAVN
                     LFLNKSNVQIYNEDYSEILNKLDNKCFVYLDPPYHPVSESSNFTGYVQGGWNIYDQIR
                     LKEACDLLTDKGIKFLLSNSFSPQILDLYNGYTINKVKANRSINSIGSERGEVDEVLI
                     RNYE"
     gene            5119..6414
                     /locus_tag="PLF48_01550"
     CDS             5119..6414
                     /locus_tag="PLF48_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48035.1"
                     /translation="MSDSKNDIAWEKLFIKYKILEQITKAGHFEISASKINEFREARL
                     MTKFDHKSQLPKLFSQNNLSILPTSRGGYCIGTFDAYYHFKDEPIEITQIEFSPVLES
                     LNFRDITSEATAINCTFVTRILQNFTNEEELFPTVSGRMSSSSFQFMINSAIGSFNIN
                     VENAQVEIDGGFEGEKSLNLIEAKNYISDDFLIRQLYYPFKLWTGKIEKQVRPIFLTY
                     SNGIFHLREFSFTKINHYNSIVLLRQKKYTLIDCRFNLEMLIQLIDKTKTVAEPEIPF
                     PQADNFNRVINLCELIGQNQTMTKEQYLQDFDFGAIDPRQHDYYTNAARYLGLVEKTI
                     DPVNKQTSYILSNLGKKIMNSPLSDRQKEFIRLILAHKVFKLSLKLYLDTSQVPAKEQ
                     IVEIMRNSKLYHIESDSTFYRRASTILSWVSWIINQIEE"
     gene            6470..7552
                     /gene="carA"
                     /locus_tag="PLF48_01555"
     CDS             6470..7552
                     /gene="carA"
                     /locus_tag="PLF48_01555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNF48036.1"
                     /translation="MTPAILLLQDGTHFTGFACGKIGTATGEICFNTSMTGYQELFTD
                     PSYFGQLLITTNVFVGNYGIKESESQSDNIQIAGLICREYNNNFSRKQADESVEDYFT
                     HNNLVGITGVDTRAVVKHVREQGAMNAIISSEITDIQELTRRLNDVPDMEGLELSSRV
                     STKATYEVGDKSAKYKIAVLDLGVKKNILKNFTDRDCFVKVFPAKTTYAEMQQFEPDG
                     YFISNGPGDPGVMDYAVDTVKEILQAEKPLFGICLGNQILARAVDIPTYKLHHGHRGG
                     NHPVKNLHTGKGEITAQNHGFAVNHEELEKSVKAKLTHFNLNDNTVEGLEVLGKKAFS
                     VQYHPEAAPGPHDSWYLFDQFVGMLD"
     gene            7699..8619
                     /locus_tag="PLF48_01560"
     CDS             7699..8619
                     /locus_tag="PLF48_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF48037.1"
                     /translation="MSKKTLYLLGILLTIIIGTWLYHKYCCHTCSGCCSGEQNTALAT
                     TGDYAPFALQNGDFSYQCYDNFKFKKDEFKHLEPVSDSINTGILALKDAIAKNPNYKL
                     LLTGYCTKDEKNTSAFPNLGFARANDVKNYLVSKGIPAANLDINGEVKDSWLIQGDTV
                     LGPVRYELQQTTTSSTTTTTTTTTEDWSALKEKINANPMTLYFNTNQSEINLTAEERQ
                     KVADISKYLDKVSGAKVNCVGHTDNVGNRDKNIQLGQERADFAKAYLIKNGISSDKIN
                     SSSKGPDEPIADNGTAEGRAKNRRTVVTVE"
     gene            8683..9477
                     /locus_tag="PLF48_01565"
     CDS             8683..9477
                     /locus_tag="PLF48_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48038.1"
                     /translation="MCWCLLIPILVGLISALLGYLLGKLLSKNDDELNALKSELTLTK
                     TDRDKHLSFVNTLQTDVANWKSKHEGLLTELDALKSKITLVTGDDDADKAKIAKLTAD
                     LAACNEKLKSATSDDASDKSAITKLQAELDACKANSTSLHANINALQGEISSLKLAAN
                     TPKLIPFDAAAALAVFGKKIAENDLKLVEGIGPKIEELFHAAGIKTWKELSETSIERC
                     REILDAGGERFQMHDPATWPRQCALMYEGKWQELKDWQDKLDGGKE"
     gene            9638..11137
                     /gene="cysS"
                     /locus_tag="PLF48_01570"
     CDS             9638..11137
                     /gene="cysS"
                     /locus_tag="PLF48_01570"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNF48039.1"
                     /translation="MSELYIYNSLSRKKEKFEPLNPPFVGLYVCGPTVYSDVHLGNVR
                     SFTTFDMVYRYLKYLGYHVRYVRNITDAGHLTNEQGEGKNRMEDQAKLEQLEPMEIVQ
                     KYTVGFHEVMQLFNNLPPTIEPTATGHILEQIEMAEQLIENGVAYEANGSVYFDVKAY
                     NEKGHNYGELSGRNVDELIAGYRDLDGQDEKKNSIDFALWKKASPEHIMQWSSPWGKG
                     FPGWHLECSVMSTKYLGKQFDIHGGGMDLKFPHHECEIAQNVGACGLKPVRYWMHGNM
                     LNFNGQKMSKSLGNSILPIELITGNHQLLDKAYSPMTVRMLFFSSHYSSELDINIKGL
                     QDAEKNLRKLMNAAKYLQEIKQEGELKPDPELEVEIMSLCLGCKESMDDDFNTAKTIA
                     NLLGLASHINTFYNGGKTANTISVDAYNLMQKNYVEFLFDILGFKEEENNGNNEVLNK
                     VMNLVIDIRKAARENKDWPTSDKIRDGLTHAGIIIKDEKDGTTFEVSNA"
     gene            11130..12191
                     /locus_tag="PLF48_01575"
     CDS             11130..12191
                     /locus_tag="PLF48_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNF48040.1"
                     /translation="MHSYLVHYLIVNSNKTNMPQYTIFKSYYFRLLLALLMPALMSPS
                     CKTKSQEETAEKTNETSIVKPAFSADSTFQFIEDQLAFGPRVPGTSAQQKCAAYFESK
                     LKQYTTHVIVQKTNVKVYNGKSVPCINVIASFNPEVKRRLLILSHWDSRPFADQGDSA
                     VNKPILAADDGAGGCAVMIEIARQLYAKDPQIGVDLLFVDVEDYGQPEYELNQEQGDF
                     YCLGTQYWCKHPHVPNYKADNGILLDMVGAKDATFTYEGVSYQYAQSFMKEVWKNAHQ
                     LGWSNLFLTNVTDQIIDDHYYVNTMAKIPTIDIIYRSFATTSGFAPHWHTHDDNISII
                     SKTTLQAVGETVLSTIYLY"
     gene            complement(12213..13280)
                     /locus_tag="PLF48_01580"
     CDS             complement(12213..13280)
                     /locus_tag="PLF48_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48041.1"
                     /translation="MDCQKDNIEVLSTIDHIRLKPGMYIGDLYYYGIWTIIYNTTIQF
                     FEKSKDGFYIEIITNRNRIKLIYVFNNTKYFYSNIHRINIIEYFSTTIGIPLVIALSK
                     ECIIKADFGNKTALVISENGNLGIPVYNTGTESRNKVALDFKIDDSILRSKEINFDYL
                     CQKIQQIAYTANNCTFVCRDMPTKQSRKFIYKNGLADLFDYQLKKDFYKYEYKNLFFK
                     TVIDEYEYEICICLNGGKYGKDNFTFANHFHTTLGGSLENSIQKGIIKAINKWTLLNF
                     NKSAFKYHISKIEYLKNTLFIVAGIKGNQLTFYGPTKETLGMPEIEKKLTQYIYKKVL
                     EYLNFEKESSIKLLKTLYIIY"
     gene            complement(13380..16256)
                     /gene="carB"
                     /locus_tag="PLF48_01585"
     CDS             complement(13380..16256)
                     /gene="carB"
                     /locus_tag="PLF48_01585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNF48042.1"
                     /translation="MENPLKMPKDHSIKSVLIIGSGPIIIGQACEFDYSGSQAARSLR
                     EEGIEVSLINSNPATIMTDSVTADHIYLKPLSIDSIRDILEERHIDAVLPTMGGQTAL
                     NLCKEAEELGLWEKYNVRVIGVDLNVIETTENREAFRKFCIDLGLAVAPSYIANSMLE
                     GKEAAQKIGFPLVIRPSYTLGGTGGGFVHKADEFEVALSRGLQASPVHEVLVEKAVLG
                     WKEFELELLRDANDNVVIICTVENLDPMGVHTGDSITVAPAMTLPDTAYQEMRDQAQK
                     MMRALGNFAGGCNVQFALNPETEELIVIEINPRVSRSSALASKATGYPIAKIASKLAI
                     GYNLDELNNQITKTTSAFFEPALDYVIVKIPRWNFDKFYGCDDKLGLQMKSVGEVMGI
                     GRNFAEALQKACQSLENNKIGLGADGKTSYKVQDVLDALEHPSWDRIFKIKEAISLGV
                     PIHSIHKLTGIDNWFLHQIQDLVKLEKSIQKYSIQNIPKALLFQAKKSGYSDLQLAYL
                     LGRIDEEDVYEKRKEFGIRRVYKMVDTCAAEFEAKTPYFYSTFENPSYNADGELQFNS
                     ESIPNKNQSKGSVIVLGAGPNRIGQGIEFDYCCVHGLLALKEEGYEAIMINCNPETVS
                     TDFDIADKLYFEPVFWEHLYELIEWEKPIGVIVQLGGQTALKLSKRLEEKGIKIIGTS
                     FDDMDIAEDRGRFSDLLKELDIPYPDYGTATDADEALAVANKVGYPVLVRPSYVLGGQ
                     RMRIVINDEECETAVINLLKDIPDNVILIDHFLDRAKEAEIDAICDGENVQIMGVMEH
                     IEPAGIHSGDSNSVLPPYSLGPIVIETMKHYTERLCKALNIRGLINIQFAIKGEKVYV
                     IEANPRASRTTPFICKAYQIPYLNIATKVMLGTHKLKDFTYSEKLTGYAIKEPVFSFD
                     KFPGVNKILGPEMKSTGEAIYFIKDLFDPYFRHLYKEKSMFLNR"
     gene            complement(16574..16750)
                     /locus_tag="PLF48_01590"
                     /pseudo
     CDS             complement(16574..16750)
                     /locus_tag="PLF48_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            16707..>17752
                     /locus_tag="PLF48_01595"
     CDS             16707..>17752
                     /locus_tag="PLF48_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF48043.1"
                     /translation="MGIYLRTSPFVFDDAEQARALFADEYVDNIYSRFSNPNVNEFVE
                     KMCVLEKMEDGFGTATGMAAVFASIMPFLNAGDHILASSALFASSTRIIKEFLPKWGI
                     ESSFFDINHPETWESKIQPNTKMIFAETPSNPGLVLIDLAFLCALGKKHGILVNIDNC
                     FATPYLQNPADYGADIITHSATKFIDGQGRVVGGVVLANKELIKEVRLFCRQTGPSMS
                     PFNAWVLSKSLETLHVRMDRHCENALALAQWLATHPEIEDVKYPFLPSHPQYALAKSQ
                     MKQGGGVVTFVVKGGLARGRNFLDSLDLFSHTANLGDCRTIATHPASTTHSKIDEATR
                     KAAGIEDGTIRVSVG"
BASE COUNT         5968 a         3141 c         3457 g         5186 t
ORIGIN      
        1 tttcccgcaa ctttgtgtgt atcaccaaac gaacaagtag ttcacggaat tccatccgac
       61 agagaaatca aagaaggaga tattgtatct attgactgcg gagtgtgtaa agacgggtac
      121 tacggcgatt cagcctttac attcccaatt gccgatatta caccggaaat aaaacgtttg
      181 ttgcaaatca caaacaattc tttatatctg ggtatagaaa aggcagtagt cggaaatcgt
      241 attggtgatg taagttttgc tgtccagaaa tacaccgaaa tagataataa gtacggtgta
      301 gtgcgcgagt taatcgggca tggagtgggc aaaaacctgc atgaagaacc ggatgttccc
      361 aattacggta aaagaggcaa aggacctgtt atgcatgaag gtttggtgat agccatcgaa
      421 ccgatgatta acatgggtac ttaccaggtg cgacaattga gcgataaatg gactgtggtc
      481 acaaaagacg gtaagccatc cgcacatttt gaacacacta ttgccgtaaa aagaggaaaa
      541 gctgacatac ttagtgacca taatttaata tacgaagcca taaaaaaatc ttcatatatt
      601 aaggatttca gagattaatt agtatctttg cactccaaaa ttatgggcaa aacagacctt
      661 ataaaattag atggtacgat cacggaagcg ctttctaacg caatgttcag agtaaagctc
      721 gaaaacggcc acgaaatctt agccacaatc tccggcaaaa tgaggatgca ttatatccgc
      781 attcttcccg gtgataaagt tgccgtagaa atctctccgt acgatctatc cagaggcaga
      841 ataacattca gatacaaata aaaaaataaa cttttatacc atgaaagtta gagcatcagt
      901 taaaaaaaga agcgaagact gcgttattgt acgcagaaag ggcagagtgt atgtcatctg
      961 caagaagaat cctaaattta aacaacgtca aggataatta tggctagagt atcaggtatt
     1021 gaccttccca aaaacaaaag aggatatata ggattaacct atatatatgg tattggtcgc
     1081 tcttcagcaa gagaaatttt ggctaaagcc gaagtagacc cggaaaagaa agtacacgaa
     1141 tggaatgatg atgaggtgac ggctatccgt aatatcatca acgccgattt caaagtagaa
     1201 ggagctttac gttctgaagt acagttgaat atcaaacgtt tgctggatat cggatgctac
     1261 agagggattc gtcaccgtaa aggtcttccg gttagaggac aaagtacaaa acgcaacgca
     1321 cgtactcgta aaggtaaacg taaaactgtt gcaggtaaaa agaaagtaac taaataatct
     1381 atcatcttag taatcgttta aagatggcta cagcacaaaa aaaagcagca aagaaaagag
     1441 tagtaaaagt agaagcagaa ggatatgttt atatcaaagc tacttttaat aatattatta
     1501 tttcctatac taataaggca ggccaggtaa tttcatggag cagcgccgga aaaatgggat
     1561 tcagaggttc taaaaaaagc accccttatg ccgcacagtt ggcatctcag gatgcaggta
     1621 ctaaagctta tgaagcagga atgcgcaaag cagaggtatt tgtaaaaggt ccggggggcg
     1681 gaagagagtc tgccatacgt tctatcggaa attcaggaat tgaagttacg attatcagag
     1741 atgtaactcc attaccgcac aatggttgtc gtccaccaaa acgcagaaga gtataagaaa
     1801 ttataaatgt tggattataa attaggtatt aatttaaaat ctaaaatcta aaatttaaaa
     1861 tcatggcaag atatacagga ccacgtacaa aaaaatctag agctttcggt gagccgattt
     1921 tcggatacga taaaagttac gaaaaaagaa aatatcctcc cggacaacat ggtaacggca
     1981 gaaaacgttc ttctcaatct gagtatgcta ttcagctgaa agaaaaacaa aaagcaaaat
     2041 atacctacgg tgtgttggag cgtcaattct acaaaacatt tgaagaagct tctcgtaaac
     2101 acggtgttaa aggcgaaaac ttgttgcgtt tattggaaca acgtcttgat aatacggttt
     2161 accgtttggg tctggcgcct acaagaagtg ctgcacgtca gttggtaaca cacaaacata
     2221 ttaccttaaa cggacgtatc gtaaatattc cgtcttgtac agtaaaagta ggtgatgtaa
     2281 tcagcgttcg tgaaaaatca aaaggattgc ctttggtaca ggatacggta gcaactggca
     2341 gagtaaaaaa atatccttgg ttggaattca atactgaaaa actggaagga aaatatgtgg
     2401 aaattccaaa cagagatcag attccggaaa ccattaaaga acaattaatt gtagagttgt
     2461 atagtaagta atgttaaaag ataatgttgg attttaataa attcacatca atcaccaaaa
     2521 cattatacat taatcactaa tcattaatca ttaaaatatg gctatattat cgtttcagaa
     2581 accggataaa attttgttgc agaaagcaac agactttgag ggtacatttg aatttgctcc
     2641 cttagagccg ggctttggaa ataccatagg caatgcactt cgtcgtgttt tgctttcatc
     2701 tttagaaggc tatgcaatta catcagtaaa aattgcaggt gtcaatcatg aattttctac
     2761 gatcaaaggt gtaattgagg atgtaacaga agtagtactc aatttgaaac aagtacgtct
     2821 gaaaagagtg ttgcaagact ctgaagccgg ttttgaaaaa ttatatattt cgttgaaagg
     2881 caaagagcaa tttttggcaa gcgatatcga atcacataca aacgtgtttg tagtaactaa
     2941 tccggaatta gttatttgca atatggagaa aaatgtgaac ctcgaaatgg aaatcaccat
     3001 aggaaaaggc agaggatatg tatctgctga agatcattac gtagataatt ctattgtagg
     3061 cttaattcct gttgactcca tttatacacc aatcaagaat gtacgttaca aagtagaaaa
     3121 tacacgtgtt gaacaacgta ctgactttga gaaattgatt attgatgtaa aaacagatgg
     3181 cactattcat ccggaagatg cagtaaaaga agccgctaag attctgattc agcatttaat
     3241 gttggtatct gatgaaaata ttacattcaa cacagaagat aaagctcaag aagaagttgt
     3301 agatgaacac atcttacaca tgcgcaaaat tttgaaaacg ccgttggaag atttagattt
     3361 atctgtacgt gcttataact gtttgaaagc ggctaaaatt aattcattgt ctgaattagt
     3421 gcagtacgat acaaatgagt tattgaaatt cagaaatttc ggaagaaaat cattggtaga
     3481 aatcgaagaa ttaatcatcg aaaaaggatt acacttcggt atggatttat caaaatttaa
     3541 attggaagac taaattaaaa gttggcaatt tgcaaatgat aagtttgcaa actgcataaa
     3601 atatttgcaa actgcaaact aaataacatg agacacggaa ataaaattaa tcatctcgga
     3661 agaaaatacg gtcacagaag tgcgttgtta aaaaacttgg cagtggcttt aatcgttaac
     3721 aaaagaattg aaacgactat tgccaaagct aaagaattgc gcggttatat tgaaccatta
     3781 attactaaag ctaaagaaaa tacgacaaca aacagacgta ctgtattctc ttatttacaa
     3841 gataaaacag ctgtaaaaga attatttgat gtcatttctg caaaagtagg cagcagaccg
     3901 ggaggttata cccgtatagt tcgcttaggt ttccgtcagg gagatgctgc tgaagtagcc
     3961 attattgagt tagtagattt caacgaagtt tacaccccaa aatctggtgc aacagcagag
     4021 ccgaaaaaga aaactcgtcg tgcgggtggt gcgaaaaaga aagcagaaac agcagaagta
     4081 gtagaagaag tgaaagcaga agaaacacaa acagcaccgg aagccacgac agaagaaaaa
     4141 acagaagaat aaatggaaat gattttttca acacatataa aaggttcgtc aaacatgacg
     4201 aacctttttt ttgaaatgat aaaaacaatt cttgggatta aggaaatgat ttttataatt
     4261 ttatgtcgta aataattgca gtagtgacaa aacaaaataa gttagtcgca ccttttttaa
     4321 agtgggttgg tggtaaaaga caacttatgt ctgctattgt gcagttgctt cctaaaaata
     4381 taaaggagct taaatacacg gaaccattta taggtggagg tgcagttttt ttttatttac
     4441 aacctcaaaa ggctataatt aatgattgca atcaagaatt aattaatgta tataatgtgg
     4501 ttaagaacca attggaggat ttaattattg atcttaagaa acatgaaaat acatcagact
     4561 atttttataa gataagaggt cttgacaggc ttaatacatt taataaacta ccggcattgc
     4621 aaagagcttc cagggttatt tatctaaata agacttgttt taatgggtta tacagagtga
     4681 ataatgcagg tgaatttaat gcaccgtttg gtcgatataa aaatccgaat atcgtaaatg
     4741 agccaatgtt aagagccgta aatttatttc taaacaagtc gaatgtgcaa atttacaatg
     4801 aagattactc agaaattctt aataaattag ataataaatg ttttgtatat ttagacccgc
     4861 cctatcatcc ggtttcagaa agctcaaatt tcactggata tgtacaaggt ggatggaata
     4921 tttatgatca gattaggtta aaagaggctt gtgatcttct gacagataaa gggataaagt
     4981 ttttgctttc aaattctttt tctccacaaa tactagattt gtataatggc tatactatta
     5041 ataaggtaaa agctaataga agtataaact ccattggaag tgagcgtgga gaagtagatg
     5101 aagtactaat cagaaattat gagtgattct aagaatgata ttgcttggga aaagttgttt
     5161 ataaaataca aaattctcga acaaattaca aaagcaggtc attttgaaat ttcggcttca
     5221 aaaataaatg aattccgaga agctcgatta atgacaaagt ttgatcataa atcacaacta
     5281 ccgaagttgt tttctcaaaa taacttgtca attttaccca catccagagg cggatattgt
     5341 attggaacat ttgacgccta ttatcatttt aaagatgaac caatagagat aacacaaatt
     5401 gagttttctc ctgttttgga aagccttaac ttccgtgata taacaagcga ggcaactgca
     5461 attaattgta catttgtaac aagaatattg cagaatttta caaatgaaga ggaattgttt
     5521 cctactgtta gtggaagaat gagttcttca tcttttcaat ttatgattaa ttcagctatt
     5581 ggtagtttta atataaatgt agaaaacgca caagtcgaaa ttgatggagg ttttgaagga
     5641 gaaaaatcat taaatcttat tgaagcaaaa aactatattt ctgacgattt tcttatccgg
     5701 cagttgtact atccatttaa actttggaca ggaaaaatag aaaagcaagt acgcccgata
     5761 ttcttgacat attcaaatgg gatttttcat ttaagagaat tttcattcac aaaaataaac
     5821 cactataatt ctatagtttt actaagacaa aaaaaatata cgctaataga ttgcaggttt
     5881 aatcttgaaa tgctgattca acttatagat aaaactaaga ctgtagcaga gccagaaatc
     5941 ccgtttccac aagcagataa ttttaataga gttattaatc tttgtgaatt gattggtcaa
     6001 aatcaaacga tgacgaaaga acaatattta caggattttg actttggcgc aatagaccca
     6061 agacagcacg actactatac aaatgctgct cgatacttag gattagttga aaagacaatt
     6121 gatcctgtaa acaaacaaac atcttatatc ttatcaaatc ttggaaagaa aataatgaat
     6181 tcaccactct cagacagaca aaaggaattc attaggctaa ttcttgctca taaagtattt
     6241 aagctttcac tgaaactata tcttgataca agccaagtgc ctgcaaagga acaaatagtt
     6301 gaaattatga gaaactcaaa actttatcat attgaatctg attctacttt ttatcgcaga
     6361 gcatcaacaa ttcttagctg ggtaagttgg ataataaatc aaatagaaga ataaccaaga
     6421 taagtaaaaa gaacaattta aaattaacca ttcagaattt ataatgacca tgacaccagc
     6481 catattacta cttcaagacg gaacacattt tacaggtttt gcctgcggaa aaatcgggac
     6541 agctaccggc gaaatttgct tcaatacctc catgacaggc tatcaggaac tgtttacaga
     6601 ccccagctat ttcggacagt tgctcattac gaccaatgta ttcgtgggca attacgggat
     6661 taaagaaagc gaaagccaat ctgataatat acagatagcc ggtttgattt gccgcgaata
     6721 caataataat ttttcacgca agcaagcaga tgaatcagtg gaagattatt ttacgcacaa
     6781 caacctcgta ggtatcaccg gtgttgacac ccgtgcagtg gtaaaacacg tgcgcgaaca
     6841 gggtgcgatg aacgccatta tctcatcgga aataacagat attcaagaac ttacaagaag
     6901 actgaacgat gtgccagata tggaaggatt ggagttgtca tcaagagttt cgaccaaggc
     6961 aacctacgag gtgggcgata aaagtgccaa atacaaaata gccgttttag atttaggggt
     7021 gaaaaaaaac atcttgaaga attttacaga cagagattgt ttcgtaaaag tatttccggc
     7081 taaaaccaca tatgctgaaa tgcagcaatt tgagcccgat ggctacttta tttccaacgg
     7141 acccggcgac ccgggtgtga tggattatgc cgttgataca gtaaaagaaa tattgcaagc
     7201 cgaaaaacct ctttttggca tttgtttagg caatcagatt ctggctagag ctgttgacat
     7261 tcctacttat aaattgcatc acggccaccg cggaggcaac catccggtga aaaacctcca
     7321 taccggaaag ggagaaataa ccgcacaaaa ccatgggttt gctgtgaacc atgaggaatt
     7381 ggaaaaatct gtcaaagcca aacttacgca tttcaattta aacgataata ccgtagaagg
     7441 cctggaagta ttgggcaaaa aagcattttc tgtacaatac cacccggaag ccgcacccgg
     7501 tccacacgac tcttggtact tatttgacca gtttgtgggg atgttggatt aatgtatgta
     7561 aaagtatttt ttataaaata ttatagttag atatacacta ttgtacacat tatatatact
     7621 tcttatgtaa atttttttct aaataatttg acaagtatca aattttagct gtaacttcaa
     7681 ttatcaaaat ttggcactat gtctaaaaaa acgctttatc tcctcgggat tttgcttacc
     7741 attataatcg gtacctggct ctatcataaa tattgttgcc atacttgctc cggctgctgc
     7801 tcgggggaac aaaatactgc acttgcaaca accggcgatt acgcaccctt tgctttacag
     7861 aatggagatt ttagctatca atgctacgat aattttaaat tcaaaaaaga tgaattcaaa
     7921 catcttgagc cggttagcga ttctataaat acgggcatac tcgctctgaa agacgccata
     7981 gccaaaaacc caaattataa attactcctc accggatatt gtactaaaga cgagaaaaac
     8041 acttcagctt ttcctaatct tggatttgca cgggctaatg atgtaaaaaa ttatctggtt
     8101 tcaaaaggta ttcctgccgc taacttagat atcaatggtg aagtgaagga cagttggctt
     8161 attcaaggtg atacagtttt gggtccggtt cggtatgagt tgcaacaaac aacaacaagt
     8221 tctacaacaa caacgaccac gacaactacc gaagactgga gcgctttgaa agaaaaaata
     8281 aatgcaaatc cgatgacttt atattttaat acgaatcagt ctgaaataaa tttgaccgct
     8341 gaagaacgcc agaaggtagc cgatatttca aaatatctgg ataaagtttc cggagcaaaa
     8401 gtaaattgtg tagggcatac agataatgta ggcaacagag ataaaaacat acagctcggt
     8461 caggaaagag cagattttgc taaagcctat ctgataaaaa atggcatcag cagtgataaa
     8521 atcaatagca gttctaaggg tcctgacgaa cctattgcag acaacggcac agcggaagga
     8581 cgcgctaaaa acagacgaac cgtggtaact gttgaataat gataaacagt aatcattaac
     8641 tcttaaacta ataaattctc aaactcttaa actaataaaa atatgtgttg gtgtctttta
     8701 attcccattc tagtcggctt aatcagtgca ttattaggct atcttttagg taaattattg
     8761 tctaaaaatg acgatgaact caatgcactt aaatcagagc ttacgcttac aaaaactgac
     8821 agagacaaac atttgtcgtt tgtaaacaca ttacaaacgg atgttgcaaa ttggaaatct
     8881 aagcatgaag gattattaac agaattggat gctttaaaat caaaaatcac tttggtaacc
     8941 ggagatgatg atgcagacaa agccaagatt gccaaactta cagctgattt ggccgcttgc
     9001 aatgaaaaac tgaaatcggc aacatcggat gatgcaagcg ataaatctgc tattacaaaa
     9061 ttacaagcag aactagatgc atgtaaagcc aactctactt cgttgcatgc aaatatcaat
     9121 gcacttcaag gtgaaatcag ttcacttaaa ttagcagcaa atacacctaa attaatccca
     9181 tttgatgcag cagcagcatt ggctgttttt ggtaaaaaaa ttgcggaaaa tgatttgaaa
     9241 ctagtggaag gaatcggacc aaaaatagaa gaactattcc atgcagcagg gataaaaaca
     9301 tggaaagaac tttcagaaac ttcgatagag cgatgcagag aaatattaga tgctggtggt
     9361 gagcgtttcc aaatgcatga tcctgccacc tggcctcgcc agtgtgcttt gatgtacgag
     9421 ggaaaatggc aggaacttaa agattggcaa gataaattag acggcggaaa agagtagtat
     9481 tttattcttc tgaaaggata aaaaaatatg cagaaaacgg ggcgcgaagc accccgtttt
     9541 cttatttgga gtaaatagta cgcatacaaa tccagattca aaaaaaatct tattacttct
     9601 tcggactact cattactaat ttttaccttt acaaaacatg tctgaattat atatttacaa
     9661 ctcgctctca cgaaagaaag aaaaatttga gccgctcaat cctcctttcg taggtttgta
     9721 tgtttgcggt cctactgttt acagtgatgt acatttggga aatgtccgat catttaccac
     9781 ttttgatatg gtttaccgtt atctgaaata tttgggatat catgtcagat acgtgcgcaa
     9841 tattacggat gccggacatc tcaccaatga gcaaggtgag ggcaagaacc gcatggaaga
     9901 tcaggcaaaa ctggaacaac tggagcctat ggaaatcgtg cagaaatata cggtaggctt
     9961 tcatgaggtg atgcaattat ttaataatct gccacccaca attgaaccca cagccaccgg
    10021 acatattttg gaacaaatag aaatggctga gcaactgatt gaaaacggtg tagcctacga
    10081 ggccaacggc agtgtctatt ttgatgtaaa agcgtataat gaaaaaggac ataactacgg
    10141 cgaactttcg ggtagaaatg tggatgaact aatagccggc taccgcgatt tggatggtca
    10201 ggatgaaaag aaaaactcca ttgattttgc actttggaaa aaagcctcgc ctgaacatat
    10261 catgcaatgg agctcgccct ggggtaaagg cttcccgggt tggcacttgg aatgctccgt
    10321 gatgagtaca aaatacttgg gcaagcaatt tgatattcac ggtggaggaa tggatttgaa
    10381 atttccacac cacgaatgtg aaattgcaca gaatgtagga gcatgtggtt tgaagcctgt
    10441 ccgctattgg atgcatggca atatgctcaa tttcaatgga caaaaaatga gcaaatcgct
    10501 gggtaattct attctaccta ttgaattaat cacaggcaat caccagcttt tggataaagc
    10561 ctattcgcca atgacggtgc gcatgttgtt cttttcatca cactattctt ccgaacttga
    10621 tatcaatata aagggcttac aggatgcgga gaaaaatctg cgcaagctga tgaacgcggc
    10681 aaaatatttg caggaaatca aacaagaagg agaattgaag cctgatccgg aactggaagt
    10741 ggaaattatg agtttatgtt tgggttgtaa agaatctatg gatgacgatt ttaatacggc
    10801 aaaaacaata gccaatcttt tgggtttagc atcgcatatc aacacatttt ataatggagg
    10861 taaaacggca aatacaatct ctgtcgatgc gtataatctg atgcaaaaaa attatgtcga
    10921 atttttattt gatatcttgg gctttaaaga agaagaaaat aatggaaata atgaggtact
    10981 gaataaggtg atgaatttgg ttatcgatat tcgcaaagca gcccgtgaaa ataaagattg
    11041 gccaacatct gataaaatca gagacgggtt gacgcatgca ggaattatta ttaaggatga
    11101 aaaagacgga actacatttg aagtgtcaaa tgcatagtta tttggttcat tatcttattg
    11161 tcaatagcaa caaaacgaac atgcctcaat acacgatatt taaatcttac tactttcgct
    11221 tgcttctggc attactcatg ccggcattga tgtcgccttc ttgcaaaacg aaatcacaag
    11281 aagaaactgc agagaaaact aatgaaacga gtatagtgaa acctgcattt tcggcagaca
    11341 gtacatttca gtttattgaa gaccagcttg cctttggccc tcgagtgccg ggaacttcgg
    11401 cacagcaaaa atgcgcggct tattttgaat caaaattaaa gcaatatact actcatgtta
    11461 tcgttcagaa aacgaatgtg aaagtatata atggcaaaag tgtaccctgt atcaatgtga
    11521 ttgcttcatt caacccggaa gtaaaaagaa gattgctgat tttgtcgcat tgggattcac
    11581 gtccctttgc cgatcagggc gacagtgcgg taaataagcc gattttagcc gcagatgatg
    11641 gtgccggcgg ttgtgcagtc atgatagaga tagctcgtca gttatatgca aaagacccac
    11701 agattggagt ggatttattg tttgtagatg tggaggatta tggccagccg gagtatgagc
    11761 ttaatcagga gcagggagat ttctattgtt tgggtacgca atactggtgc aaacatccgc
    11821 atgtgcccaa ttataaagcc gataacggaa ttttgctcga tatggtgggt gctaaagatg
    11881 ccacatttac ctacgaaggt gtttcttatc agtatgcaca aagtttcatg aaagaagttt
    11941 ggaagaatgc ccatcagttg ggttggagta atcttttttt aaccaatgtt accgatcaga
    12001 ttattgatga ccattactat gtaaatacaa tggccaagat tcctaccatt gatattatct
    12061 atcgttcatt tgcaacaaca agtggttttg caccacactg gcatacccac gacgacaata
    12121 tcagtattat cagcaaaacc accttacaag ccgttggtga aaccgtattg agtacgattt
    12181 atttgtattg aaactaagtt tttaaaaaat tactaatata ttatgtaaag tgtttttagt
    12241 agttttattg agctttcttt ttcaaaatta agatactcaa gtactttctt ataaatatac
    12301 tgagttagtt tcttctcaat ttccggcatt cctaatgttt ctttagtagg gccgtagaaa
    12361 gttaactgat tgccttttat accggctacg ataaataaag tattctttaa atattctatt
    12421 tttgatatat ggtatttgaa agcactttta ttaaaattca ataatgtcca tttgttaatt
    12481 gctttaatga ttcccttttg tatagagttt tctaaagacc cacctaatgt tgtatgaaaa
    12541 tgatttgcaa aagtaaaatt gtctttccca tattttccgc catttagaca gatgcaaatt
    12601 tcatactcat attcatctat aacagttttg aaaaataaat ttttatattc atacttataa
    12661 aaatctttct ttaattgata atcaaataaa tcagctaatc cattcttata aataaacttt
    12721 ctggattgtt ttgttggcat atccctgcaa acaaaagtac agttatttgc agtatatgct
    12781 atttgttgta ttttctgaca taagtaatca aaattgattt ctttagacct aagaatgcta
    12841 tcgtctattt taaaatctaa agcaacttta tttctggatt cagttcctgt attatatact
    12901 ggtattccaa ggtttccatt ttcagaaatt actaaagctg ttttgtttcc gaaatctgct
    12961 ttgattatgc attctttact gagtgcaatt actagtggaa ttccaattgt agttgagaaa
    13021 tattctataa tattaatcct gtggatatta gagtagaagt atttagtgtt attaaataca
    13081 tatattagtt ttatcctatt tctatttgtg ataatttcta tataaaaacc atctttagac
    13141 ttttcaaaga attgaattgt cgtattatag ataatagtcc atatgccata ataataaagg
    13201 tcaccaatat acatgcctgg tttcaatcta atatgatcta ttgtgcttaa tacctcaata
    13261 ttgtctttct gacaatccat ttttatttaa ttttacacat attaataacg caataagata
    13321 cgaaatttta taaatatgat caaataaaat agggtaattg tctgttaata ttagactctc
    13381 tatctgttca aaaacatgct cttttccttg tacaaatgtc tgaagtaagg gtcgaacaag
    13441 tcttttataa agtagatagc ctctccggta gatttcatct cagggcctaa tattttattt
    13501 actcccggaa atttatcaaa cgaaaatacc ggctctttga tggcgtaacc ggtcagcttc
    13561 tcggaatagg taaagtcttt caacttgtgt gtacccagca tcactttggt tgcaatattc
    13621 aaatatggga tctgataggc tttgcaaata aatggtgtcg ttcgcgatgc acgcgggttg
    13681 gcttctataa catatacttt ctcgcccttt atggcaaact gaatattgat taatcctcgt
    13741 atgtttagtg ctttacacaa tcgctcggta tagtgcttca tcgtttctat cacgataggc
    13801 cccaagctgt aaggaggcaa tacagaatta ctgtcaccgc tatgtatgcc tgccggttct
    13861 atatgttcca tcacccccat tatctgcacg ttttcaccat cacaaatggc atcaatctcc
    13921 gcttctttgg ctcggtctaa aaagtggtca atcaaaatca cattatccgg gatatctttc
    13981 aaaagattaa tgaccgccgt ttcgcactct tcgtcattaa taacaatacg catgcgctgc
    14041 cctcctaaca cgtagctcgg acgaactaaa acgggataac cgactttatt ggcaacagcc
    14101 aacgcttcat cggcatctgt tgccgtgcca taatcggggt aggggatatc aagttctttt
    14161 aataaatcag aaaaacgacc gcggtcttcg gcaatatcca tatcgtcaaa agaagtgcct
    14221 atgattttta tgcctttttc ttctaatcgt ttggataatt tcaaggctgt ttgtccaccc
    14281 aattgtacaa tgacacctat cggtttttcc cattcaatta attcatatag atgctcccaa
    14341 aaaacaggct cgaaatagag tttatctgca atatcaaaat ctgtagatac cgtttccgga
    14401 ttgcaattta tcatgatggc ttcatagcct tcttctttta atgccagcaa cccatgcaca
    14461 cagcaataat caaattctat cccttgccct atgcggttgg ggccggcacc taatacaata
    14521 acagagcctt tgctttggtt tttatttgga attgattcag aattaaattg caattctcca
    14581 tcggcattat acgatggatt ttcaaaagta gaatagaaat acggcgtttt ggcttcaaac
    14641 tcggcagcgc aggtatctac cattttatag acacggcgaa tgccaaattc tttccgtttt
    14701 tcatacacat cttcttcatc tattcttccc aataaatacg ccaattgcaa atcggaataa
    14761 ccggattttt tagcctgaaa taataaagcc tttggaatat tttgtatcga atatttctgt
    14821 atcgatttct ccagttttac gaggtcttga atttggtgta aaaaccagtt atctatgcct
    14881 gtaagtttgt gtatggaatg gatgggaacg cctaatgaaa tagcttcttt aatcttaaaa
    14941 atcctgtccc agctcgggtg ttccagagca tccaaaacat cctgtacttt atacgatgtt
    15001 ttgccatccg cacccaatcc aattttattg ttttctaaag actgacaagc tttctgcaaa
    15061 gcctctgcaa agtttctgcc gatgcccatg acctcaccta ccgatttcat ttgtaagccc
    15121 aacttatcat cgcaaccata aaatttatca aaattccagc gaggtatttt tacgatgaca
    15181 taatctaagg caggttcaaa aaaagcggat gtcgtcttgg taatttgatt attcagttca
    15241 tccaaattat agccgattgc cagcttagac gcaatttttg caatcggata gcccgttgct
    15301 ttggatgcta atgcagaaga acgagataca cgcgggttga tttcaatgac aatcaattct
    15361 tccgtttctg gattgagggc aaactgtaca ttacagcctc cggcaaaatt gccaagcgca
    15421 cgcatcatct tttgtgcctg atcgcgcatt tcctgataag ccgtatcggg tagagtcatg
    15481 gcaggggcaa ccgtaataga atctcccgta tgaacgccca ttgggtcgag gttttcaacc
    15541 gtacaaataa tcactacgtt gtcattggca tcacgcaata attccaactc gaattctttc
    15601 catcctaaaa cggctttctc aaccaaaacc tcgtgaaccg gggatgcctg caagccccga
    15661 gataatgcta cttcaaattc atctgcttta tggacaaaac cgccacccgt gccacccaaa
    15721 gtataagatg gacgaataac caaggggaaa ccgatttttt gtgcagcttc cttaccctcc
    15781 aacatagagt tggcaatata agatggagct actgcaagtc ctaagtcaat gcaaaacttg
    15841 cgaaaagctt cacggttttc agtagtttct atgacgttta aatccacgcc tataacacgc
    15901 acgttatatt tttcccataa tcccaattct tctgcttctt tgcaaagatt taaggctgtt
    15961 tgacctccca tggtgggaag tacagcatca atatgtcttt cttccaggat atcgcgaata
    16021 gaatctatag ataagggctt gagatagata tgatctgccg ttaccgaatc cgtcataata
    16081 gtagcaggat tggaatttat gagggatact tcaatacctt cttctcgtaa acttctggct
    16141 gcttgagaac cggaatagtc aaattcgcag gcttgtccga tgataatagg tccgctgcca
    16201 ataatcaata cggacttaat gctatggtct tttggcattt ttagaggatt ttccaaagat
    16261 agcaaaataa accaggatgg aataagatgg aattgtaaat tttagttatg cttaatataa
    16321 tctgaaaatg aacaacaatc taccacgaca atgctcactt gagtgctgta gccttatgtc
    16381 aagacgagac agttgtgcat tttcaattat tgccttttcc agccataact actttttggt
    16441 tgagatgtaa tttggcaggt aattgaatat gtgtgacatg attatggttg taatcatatc
    16501 acatataaaa atatgctagt atcttattgt aaaccaatat taggaattat gccattggta
    16561 ataagagctg aattcaagta ataaacgcaa ttttgttaag aataatttta tcgttgttaa
    16621 aaagagcatt tacaatttca tttggggtat aaaaaccgag tttttttctg ggtctattgt
    16681 tgagtttgtt ttcaaaggat gattttttgg ggatatatct acgaacgagt ccgtttgtat
    16741 ttgacgacgc ggaacaggca cgtgccttgt ttgccgacga atacgtggac aatatttact
    16801 cgcgcttttc taacccgaat gtgaatgagt ttgtagaaaa aatgtgcgtg ctggagaaaa
    16861 tggaagacgg ctttggtact gccacgggta tggcggcagt gtttgcatct attatgcctt
    16921 ttctgaatgc cggagaccat attctggcat cgtctgcatt gtttgcatct tctaccagga
    16981 ttattaaaga gtttttacca aaatggggca ttgaatcttc cttttttgat atcaatcatc
    17041 ccgaaacttg ggaaagcaag atacagccga ataccaaaat gatatttgct gaaacgccat
    17101 ctaacccggg attggttttg attgatttag cttttttatg tgcacttggc aaaaagcatg
    17161 gcattttagt caatattgat aattgttttg ccacgcctta tttacaaaat cctgccgact
    17221 atggtgctga tattatcacg cactctgcta ccaaatttat tgacggtcaa ggtcgggtag
    17281 ttggtggcgt ggtattagcc aacaaagaat tgataaaaga ggtgcgttta ttttgtcgcc
    17341 aaacaggacc ctctatgtcg ccgtttaatg catgggtatt gagcaaaagc ttggaaacat
    17401 tgcatgtgcg catggacaga cattgcgaaa atgctttggc tttggcacaa tggttagcaa
    17461 cccaccctga aatagaagat gtaaaatatc cgtttttgcc atcgcatccg caatatgcat
    17521 tggctaaaag ccagatgaag caaggtggcg gtgtagttac ttttgtagta aaaggcggac
    17581 tggcaagagg cagaaatttc ttggacagtt tagacctatt ttcgcacacg gccaatttag
    17641 gtgattgccg caccatagcc acacacccgg cctctacgac acacagcaaa atagatgagg
    17701 ctacacgcaa ggcggcaggc atagaagacg gcacgatacg tgtttctgta gg
//
LOCUS       DAOQII010000016        17398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1547_length_17398_cov_5.189875, whole genome shotgun sequence.
ACCESSION   DAOQII010000016 DAOQII010000000
VERSION     DAOQII010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17398
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1547_length_17398_cov_5.189875"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLF48_01600"
     CDS             complement(<1..575)
                     /locus_tag="PLF48_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNF48044.1"
                     /translation="MIYHNEKSVLFFNGEFKLVTDFKISPFNQTLHYGYGVFEGIRAY
                     NTQSGPHLFKVKRHFERLLHSAEKINIRMPYTVQEMINYAYELIERSGLKEAYIRPLV
                     FMDKNMTLLPHDAEKPNVLMTCWKWNRYYHSNHLKVLVSSWRRPHPRTMPVDTKVTGN
                     YINSILASGEAKQKGYDDALLLDVEGYIASGP"
     gene            complement(732..1403)
                     /locus_tag="PLF48_01605"
     CDS             complement(732..1403)
                     /locus_tag="PLF48_01605"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNF48045.1"
                     /translation="MAIIQEIELIKSKIGKYQKDGKIMFAGSSFQTHSIPMLHILSEI
                     DNRIPVYFLNTGYLFPETIAYRDEIASKFKLNVIDLKSPTPRNMQKDSNGRLFFTSDP
                     DFCCHLNKIQPLEPILQQFDVWINGVRADQNANRAQMKEEQQTPHRAKRYHPMLHWSK
                     KMIWDYIREYQIPHHPLDKEGYVSIGCEPCTRKIDISKMGDERMARWFGLNKTECGLH
                     TELVK"
     gene            1566..2144
                     /locus_tag="PLF48_01610"
     CDS             1566..2144
                     /locus_tag="PLF48_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNF48046.1"
                     /translation="MKKQLVLFLVACSSLLFSSCGYNSLVSLDESVKKAWGDVEAAYQ
                     RRSDLIGNLVETVKGEAKFEKDVLTSVMEARAKATSIQIKADDVNADNIQKFQEAQSQ
                     LSGALSRLLAVSENYPNLKATQAYSDLMTELEGTENRINIARKDYNEAVQAYNTKARS
                     FPTNITAGLFKFKPKDMFKSDPGTEKAPKVQF"
     gene            2154..2594
                     /locus_tag="PLF48_01615"
     CDS             2154..2594
                     /locus_tag="PLF48_01615"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNF48047.1"
                     /translation="MASANDFLKTNEKDLIVSAIRKAEMDTSGEIRVHIDSRCDNAAY
                     DRAVELFTHLNMQKTIFRNAVLIYVATEDKKFAVIGDEALNKKVHAAFWDNLAGKLHD
                     CFTEGNYCNGIIDCIEKIGQVQKKYFPDIHLLDHNELSDEISFE"
     gene            2587..3357
                     /locus_tag="PLF48_01620"
     CDS             2587..3357
                     /locus_tag="PLF48_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNF48048.1"
                     /translation="MNKLFILFVFAFTSLVNAQVLDKDGIPEPPVPPRLVNDYAGLMS
                     STNVQNLEDKLVRYNDSTSSQIVIVTLNTLDGYPIEDYAIQLARKWGIGQKDKDNGIL
                     IIVAKDDRLMRIEVGTGLEPYVTDLATKRIRDEILAPAFKQSNYFKGLDDATDRLFQL
                     MTGAFHYDESDNKSSGEFPSALFVILVILFFLFLFYLMSKSSKRGYTMSGGGWTWGGG
                     GGGGGWSSGGSWGGSSGGGDFGGFGGGGFSGGGSSGSW"
     gene            3448..3966
                     /locus_tag="PLF48_01625"
     CDS             3448..3966
                     /locus_tag="PLF48_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48049.1"
                     /translation="MTCISIFMLNACKQEQHAIEFVKITDVDLGNLKKENATLNGIAV
                     FRNNDKAATFMMNDMVLNFSVDGKDIGTIVVKNKRKIDANTEFSVPFSYKYVSGKIVD
                     AGSEPATIYAIQLLGKLNVSDGAKEQSSDVKYAESYEYKTAKEERIEKREEKREARKE
                     RREQRKEERKNK"
     gene            3963..4475
                     /locus_tag="PLF48_01630"
     CDS             3963..4475
                     /locus_tag="PLF48_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48050.1"
                     /translation="MKTKALKQYTILIFLMVSTMLAKAGDTIQTNAPNDPYLVQTKNF
                     TVKNFSLKQIDMGITAVIYNPYKVKVKIEEILIDVFIEGKKLGTITQSIGEVKIFKQS
                     AFDLPLGINVQTGPTLSKFFTEGAKLAVGKPIKVDYVGYVKIKALGFIPVKVKINDSA
                     YFTRKDVIGY"
     gene            4554..4916
                     /locus_tag="PLF48_01635"
     CDS             4554..4916
                     /locus_tag="PLF48_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48051.1"
                     /translation="MGSLKSIVLCKCPKCSEGKMFKDSNPYHLSKLTEMVDECPNCGF
                     NLKNETGFHFLSMYMSYIISCAICGIVIIPGTYLVGLDNFPYVLIFNAIFLILLMPLI
                     FRWARMLSFWATSKFKFD"
     gene            complement(4949..5791)
                     /locus_tag="PLF48_01640"
     CDS             complement(4949..5791)
                     /locus_tag="PLF48_01640"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNF48052.1"
                     /translation="MNIAIFGIGLIGGSASIDLRKANFGTHFIGVGKSKASCDKALEL
                     GLVDEILPREEAIKKADLIILTVPVNILINELTFVLDNIRAGQTVTDMGSTKGHIIDA
                     IKDHPMRMRYVASHPMAGTENSGPSAAIPNLYRNKVCIICDKENTAEDALKLVESMYD
                     TLGMRIKYMEAHQHDMHAAYVSHISHISSFVLAVTVLEKEKDEEAILEMAGGGFESTV
                     RLAKSSPDMWAPIFQQNKNYLLEVMDTYIEKMYHFRNLINKNKYEELKAFMDEANEIR
                     KILK"
     gene            complement(5846..7483)
                     /gene="pruA"
                     /locus_tag="PLF48_01645"
     CDS             complement(5846..7483)
                     /gene="pruA"
                     /locus_tag="PLF48_01645"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNF48053.1"
                     /translation="MPNKIAPIPMPQNEPILGYMPGSKERADLKAEIHRRKHTQYDIP
                     MLINGKEIRTDETSAIFPPHELAHQLGQYHKGKLEHVHLAINTALNARTKWVAMHWED
                     RAAIFLKAADLISGPYRASINAATMLGQSKNVFQAEIDAACELADFLRFNVRFASEIY
                     SEQPISAPGIRNTIEYRPLEGFIYAITPFNFTAIAGNLCASPALMGNVVVWKPSHYQV
                     YSAWTIMEIFRKAGLPDGVINMIYTDPVETTQAILTHPDFAGVHYTGSTAVFNSIWKT
                     IGENLDTYKNYPRIVGETGGKDFILAHPSADTDVLATAILRGAFEYQGQKCSACSRAY
                     IPKSLWETTWEKMYEALKTFKIGKTDDFSVIMGAVISADAYKRITNYIEQAKENPDNI
                     IMFGGEYENQKGYFIQPTVILTKNPKSVTMVEEIFGPVLTIYVYDDKETRLEDVLKLV
                     DETSEYALTGAIIAQDVYAIEQMQKALVNSAGNFYINDKCTGAVVGQQPFGGSRKSGT
                     NDKAGSKWNLLRWVTPRTVKESYTPPKYYTYPYMSES"
     gene            complement(7591..8124)
                     /locus_tag="PLF48_01650"
     CDS             complement(7591..8124)
                     /locus_tag="PLF48_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNF48054.1"
                     /translation="MRKLKLDELNRISKDAFKQAEKLPIIVVLDNVRSAMNIGSVFRS
                     ADAFLIQSIYLVGITATPPNREILKTALGATETVDWKYFPTAEACINELDKYEVDLIA
                     IEQSDDSVYLQDFIWQIGKKYALVFGNEVDGVSDVFMENCLQCVEIPQMGTKHSFNIS
                     VSAGIVLWEAAKKLAAF"
     gene            complement(8127..8627)
                     /gene="rimM"
                     /locus_tag="PLF48_01655"
     CDS             complement(8127..8627)
                     /gene="rimM"
                     /locus_tag="PLF48_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNF48055.1"
                     /translation="MELVKIGKVLKTHGFKGHVRIAFDAYYQTDFEQTIKALFIQNLP
                     YFILSKDLNSPGNAIVLLEEIDTKEKAQKICGKELFVREEELEEVFNEERYDELIGYI
                     LIDKKLGKLGKMEDIFETPQQLLAQVTYQEKEILIPLNEDFIIKIDNKKKSISLQLPD
                     GLIDSQ"
     gene            complement(8679..9209)
                     /gene="rpsP"
                     /locus_tag="PLF48_01660"
     CDS             complement(8679..9209)
                     /gene="rpsP"
                     /locus_tag="PLF48_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNF48056.1"
                     /translation="MATRIRLQRHGAKGAPFYKIVVANKRVKRDGKFIELLGTYNPTT
                     IPASIQLDVNKAVEWLQNGAEPTPTAKAILRYKGAFFKKHLLRGVKLGIVQADDVETK
                     FAAWVDAHVNKVIDHQKKAADVKQAKIDASVKAPAKVKELPVEETIETAESTETVAEA
                     TDAVENTGDATPENAE"
     gene            9738..10307
                     /locus_tag="PLF48_01665"
     CDS             9738..10307
                     /locus_tag="PLF48_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48057.1"
                     /translation="MRISSKILEVNRQKLLLAFPIDLKQDVEIVSEFLLDKNLKIDSI
                     SEQDILLNGQKLIIPRRVYFDISSVQFNNTLTNTQQTILNCIYLRHHNGFVRQEGLEK
                     LISNAEDYYIIPYIFQILGEYVVALLEIADKHINENTIDNYLKFCIENPKYRKLTESR
                     MISYWDAYYRRTKFKNLNDYLGRQIFNKL"
     gene            10393..11526
                     /gene="hemW"
                     /locus_tag="PLF48_01670"
     CDS             10393..11526
                     /gene="hemW"
                     /locus_tag="PLF48_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNF48058.1"
                     /translation="MAGIYIHIPFCKQACSYCNFHFSTLTVHRDEMIKAILAEIEVRA
                     HFFSDNTQLQSIYFGGGTPSILPVEAIQIILEKIYATFSITENPEITLEANPDDLSRA
                     YLQQLKTETKINRLSIGVQSFFEEDLHYMHRAHHALQAETAIKNALDFGFSNLTVDLI
                     YGVPTLSDEHWKKNIQLLLDFGIPHISAYALTVEDKTMLKRHIQQHKKIAPEDDKAIR
                     QYKLLIEQLEHAGFLHYEISNFAKLGFLAVHNSSYWDNTPYLGVGPSAHSYDGKNRFW
                     NIANNAQYMKAIASGNLLFEKEVLSEQDFFNEYMMTKLRTQAGVSTIDLQDRFGNMMT
                     LHFLENIQPYVQRNLVQVAQHNYRLTTEGKLLSDKIISDLFLV"
     gene            complement(11527..12510)
                     /locus_tag="PLF48_01675"
     CDS             complement(11527..12510)
                     /locus_tag="PLF48_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48059.1"
                     /translation="MEFKHLSLFLNIPLLLIGFFLLNRGAGMLIDGSTSILRKISVPE
                     TAINFTAIAFGTSLPKLFTILLSAYNGYTDLVIGTILGSCIFNLIGVLAIVGLTRPMR
                     THLTTATIGIATLLFSTVLFYFLANDVLISNRSGINFSRTDGYILLAGFVLFMTYGYF
                     NIKKHHTIWFTDEQPEQHFYPVWFAIILILVGILGLVAGGVLCVNNLIDISQKYHISQ
                     KFLGQGILSVGGSFVMLYWIISTKSHSSKFELSNLIGLDMLNLLGMTGAAALIMPFAY
                     NKVFNFDVLLFLCITILLLLLLWVGKKLTITKAKAKLLLVVLLLYLVYLFL"
     gene            complement(12644..13183)
                     /locus_tag="PLF48_01680"
     CDS             complement(12644..13183)
                     /locus_tag="PLF48_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48060.1"
                     /translation="MKKHTFRLSIDEPCLENWADMSQTEQGAFCAVCQKEVIDFSDKT
                     SSEIAAFFSQKKEKTCGRFLKRQLEETYVIYPPYRHSKFKYAAGLALGLMSTQLSHAQ
                     DSIDKNPKVVIEKSEPISTKNVDTDTIKTNIYESNINKTKPIIARHLIGRVCIQPVHD
                     SDDVYNNWYKTRIIRRKKR"
     gene            complement(13198..13866)
                     /locus_tag="PLF48_01685"
     CDS             complement(13198..13866)
                     /locus_tag="PLF48_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF48061.1"
                     /translation="MSIDEPCLENWADMSQTEQGAFCAVCQKEVIDFSDKTSSEIAAF
                     FSQKKEKTCGRFLKSQLEETYVIYPPYRKSKFKYAAGLALGLLFTNSSSAKNKLTNFE
                     KIQVVSCHTQVQDSLIETKSKKDFYTISGYVVDENGEALVGVNVTLFNSTMGTVSDIN
                     GKFTLTIDSVPAIFCFSFGGYQTKYIKLSQAYKNYSNIISGKINLEIDNSFFDTDFGI
                     VSNY"
     gene            complement(13974..14237)
                     /locus_tag="PLF48_01690"
     CDS             complement(13974..14237)
                     /locus_tag="PLF48_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HNF48062.1"
                     /translation="MSIQEKFEQAQKEVKTLTKRPSDMQLLDLYAFFKQATEGDNDTD
                     KPGMFDIKGKFKWDAWKNKAGMSSDEAMQSYIDLVDELLQSHK"
     gene            14268..15791
                     /locus_tag="PLF48_01695"
     CDS             14268..15791
                     /locus_tag="PLF48_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48063.1"
                     /translation="MISRKFQFTFLICIPLLWFFFKLLPVYTLPADCSMPNFIYPENI
                     EHLKIWCFDKHSDIRFDDSNNFLFISLMWVGMHFLKLTAIKASLWISGISVLLTVWLM
                     QRTVDTRFSSIQLLLVGFVFLSVQVWTGVLGDEILFSGMLWLFVIHAFWKHRYFGIMV
                     WGTIAIIAQVQNILLVLPLLIASYWDIKQYKERDRKRFWRRRISETVLFFMLPLGLYY
                     FYRFAYFGKVLPFDWYHHQLESDRKYWIFNVEAWNVLKHYVRYFILPLVLGILFYFVK
                     QRKKLNHKYYALFYAMILFPMLYNLTISQDENLGYKNFYSLYLGLSLLLLLFLRDFRS
                     ISMGFFAFIFVFFYGFKQSFHSFGKALQSHNQNMYYLSTDLAEIHDGSAIVYYDNFLG
                     WNTDWKIYFANGKHSKVKEEMTLENMFNSDADMLFLKEPILDYPENKFVGYSIPVNTM
                     QYVKEAEPENSLDKFFYKYEHKVPLDTRMIPILLNKRSKRFDAIKKAIENRGAVLNN"
     gene            complement(15788..>17398)
                     /gene="metG"
                     /locus_tag="PLF48_01700"
     CDS             complement(15788..>17398)
                     /gene="metG"
                     /locus_tag="PLF48_01700"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNF48064.1"
                     /translation="TISGSAPVLRKTKHWFLKLDEQQEDIKNWLEKTAAENNWKAHVV
                     GQCKSWLNDGLKPRAMTRDLDWGIPVPPELEGSEGKVLYVWLDAPIGYISASKQLFRE
                     IESGKFEFAYPENFAEKIKNKKAEDWKKYWQDDETTLIHFIGKDNIVFHCITFPAILQ
                     ATQQYILPDNVPANEFMNLEGDKISTSRNWAVWLHEYLQDFPGKEDELRYVLTSNMPE
                     NKDSEFTWKDYQDRVNNELVAAYGNFVNRVVTLIHKYYDGKLDDSFRKTMRDDDSILS
                     KTIIETELAIASSIEQFRFKDALNELMAFARSGNKFLADTEPWKLIKENPEKTKDILA
                     LSLDWVYALAVFSQPLLPKTAHKIFDMLNADVQDANPFNYQLKSDKINEAKLLFPKIE
                     DEQIAFQIEKLNKIKESRIKSQDETQSEAANTTYTELKTEIQFDDFAKMDIRTGIILE
                     AEKVPKADKLLKLLVDIGIEQRTIVSGIAEHFAPENIIGQKVSVLVNLAPRKLRGIES
                     NGMILMAEDENGKLHFVQADIKAGFGIR"
BASE COUNT         5383 a         3106 c         3132 g         5777 t
ORIGIN      
        1 ggccccgagg caatatagcc ttctacatct aacagcaatg catcgtcata tcctttttgt
       61 ttggcctcac ctgatgctaa aatggaattg atgtagttgc cggtaacttt agtatctaca
      121 ggcatagtac gaggatgagg tctgcgccag gaggacacca atactttgag atgattggaa
      181 tgataatagc gattccattt ccagcaagtc atcagcacat tcggtttttc tgcatcatgt
      241 ggcagtaaag tcatgttttt gtccataaaa accaatggcc ggatataggc ttcttttaat
      301 ccgcttcgct ctattaattc gtaagcatag tttatcattt cctgaacagt atacggcatc
      361 ctgatattta ttttttcagc cgaatgtaat aagcgttcaa aatgtctttt tactttaaac
      421 aagtgaggcc ccgactgggt attataggct cggatacctt caaatacgcc atatccataa
      481 tgtaaagttt gattaaatgg gctgatttta aaatctgtaa ccagtttaaa ttctccattg
      541 aaaaacaaaa cagatttttc gttgtggtaa atcataaaaa actcaatttc attgccgaat
      601 gaagataaaa caaatttttc agcttcgttt ataaagatat tgttatgagt gaaatcgatg
      661 aagtatatag ttggcagtat gcagtatgca aaaataaagt acggtaggca gaggactatg
      721 gactgtcaat actatttcac cagttcggta tgaagcccac attcggtttt atttaatcca
      781 aaccatctgg ccatacgctc atcgcccatt tttgagatgt caatttttct ggtacatggc
      841 tcgcaaccaa tactcacata accttcctta tctaagggat gatgtggaat ttgatattct
      901 cggatgtaat cccagatcat tttttttgac cagtgtaaca tcgggtggta gcgctttgca
      961 cgatgaggtg tttgctgttc ttctttcatt tgtgcacggt tggcattttg atcggctcta
     1021 acaccattaa tccaaacatc aaattgttgt aatatgggtt ctaagggttg aatcttattt
     1081 agatggcaac aaaaatctgg gtctgaagtg aaaaacaaac gcccgttgct gtctttttgc
     1141 atatttcttg gcgtaggaga ttttaagtcg attacattga gtttgaattt tgaggcaatt
     1201 tcatcccggt aggcaatagt ctcaggaaat aaatatccgg tattcagaaa ataaacggga
     1261 atacggttgt caatctccga caaaatatgt aacatcggga tggagtgagt ttgaaaggat
     1321 gatcctgcaa acataatttt cccgtccttt tggtatttcc cgatttttga ttttattagt
     1381 tcaatttcct gaataattgc catagttaaa aataccttta ttgatttgcc cctgtgattg
     1441 gaatatatta ataaatcatt gaaaaattgt gtacaaatta caccaaatgc gttttgggga
     1501 actaaatcga attaaaaagt gtatttatgc ggagtaaata ctatctttaa atttaaaatt
     1561 acagtatgaa aaaacaatta gttttatttt tagtggcatg ttcttcactg cttttttcaa
     1621 gctgtggcta caattcatta gtttcattag atgaatctgt aaaaaaagca tggggcgacg
     1681 ttgaagctgc ttatcagcgc agatcagatt tgattggcaa tttggtagaa actgtgaaag
     1741 gagaagccaa atttgaaaag gatgtgctga cctctgtaat ggaagcacgg gcaaaggcta
     1801 cttccattca gataaaagct gatgatgtga acgctgataa tattcagaag tttcaagaag
     1861 cacaatctca attaagcgga gctttatccc gtttgttggc ggtgtcagaa aattatccca
     1921 acctcaaagc aacacaagcc tattctgatt taatgacaga actggaaggg acagaaaacc
     1981 gtattaatat tgccagaaaa gattataacg aagctgtaca agcgtataac acaaaagctc
     2041 gttcattccc gactaatatc acagctggtt tattcaaatt taaacctaaa gacatgttta
     2101 aatctgatcc gggaacggaa aaagcaccaa aagttcaatt ctgatttttt gaaatggcat
     2161 cagcaaatga ttttctgaaa acgaacgaaa aagatttgat tgtctcggcc attcgtaagg
     2221 cagagatgga tacttccgga gagattcggg tgcatattga tagtagatgc gataacgcag
     2281 cgtatgatcg tgcagttgag cttttcacac atttaaatat gcaaaaaact atctttcgga
     2341 atgccgtgct tatttatgtg gccacagaag acaaaaagtt tgctgtgatt ggagatgaag
     2401 cgctcaacaa aaaagtacat gctgcttttt gggataattt agccggtaag ctccatgatt
     2461 gttttactga aggaaattat tgcaacggta ttattgactg tatagaaaaa ataggacaag
     2521 ttcagaaaaa gtattttccg gatattcacc ttttggatca caacgaatta agcgatgaaa
     2581 tttcatttga ataaactttt catactcttc gtatttgcgt ttacctcgct ggtaaatgct
     2641 caggtgttgg ataaagatgg aataccagag ccacctgttc ctcctcgctt agtgaatgat
     2701 tatgcgggct tgatgagtag tactaatgtt caaaacttag aagataaatt agtccgttat
     2761 aatgactcta cctcttcgca aattgtaatt gttaccctaa acacacttga cggatatcca
     2821 attgaagatt atgcaattca gctggccaga aaatggggga taggtcaaaa agataaagac
     2881 aatggcatac ttattatagt tgccaaagat gatcgcctga tgagaataga ggtaggcacc
     2941 gggctagaac cttatgttac tgatttggca actaaacgca ttcgcgatga aattcttgct
     3001 ccggcattca agcaaagcaa ttatttcaaa gggttagacg atgctaccga cagattattt
     3061 caattgatga ccggtgcatt tcattatgac gaatcagaca ataagagttc aggagaattt
     3121 ccgtctgcct tatttgttat tcttgtaatt ttattcttct tgtttttatt ttatcttatg
     3181 tctaaaagtt ctaaaagagg ctataccatg tcgggcggtg gctggacatg gggcggtgga
     3241 ggcggtggcg gtggctggtc atccggcggt agctgggggg gcagcagcgg aggtggtgac
     3301 tttggcggtt ttggcggcgg aggattcagt ggtggaggca gtagcggcag ttggtaaatc
     3361 taaaattgta ttaaaaattt ttggtatcac ttttgattat ttatttttca gaaaaaaaac
     3421 atgtacacgt atttaaaaat tgcaacattg acttgcatta gcatttttat gctgaatgct
     3481 tgtaaacagg aacaacacgc aatcgaattt gtaaaaatta cagatgttga tttgggcaat
     3541 ctcaaaaaag aaaatgctac gctgaatggt attgcagtgt ttagaaataa tgataaagct
     3601 gcaacattca tgatgaatga tatggtgctt aattttagtg tggacggcaa agatattggc
     3661 actattgttg taaaaaataa acgaaaaata gatgctaata cggagttttc cgtgccattt
     3721 tcatataaat atgtaagcgg aaaaattgta gatgccggaa gtgaacctgc aacaatttat
     3781 gcgatacagt tgcttggcaa actgaatgta tctgatggtg ccaaagagca aagcagcgat
     3841 gtgaaatatg ctgaatcgta tgaatataaa acggcaaaag aagaacgaat agaaaaacgt
     3901 gaagaaaaaa gagaagcacg aaaagaaaga cgtgaacaac gtaaagaaga aagaaaaaat
     3961 aaatgaaaac aaaagcactg aaacagtata caatcctgat atttctgatg gttagtacga
     4021 tgcttgccaa agcaggggat actattcaga cgaatgcacc aaatgaccct tatctcgttc
     4081 agactaagaa ttttacggtc aaaaactttt cactcaaaca gattgatatg ggcatcacag
     4141 ccgtaatcta caatccgtat aaggtaaaag ttaagataga agaaattttg attgatgttt
     4201 ttatagaggg gaaaaaactg ggaacaatta cgcagtctat cggagaagtc aaaattttta
     4261 aacaatctgc attcgatttg cctttaggta ttaatgtgca aacaggtcct acactttcca
     4321 agttttttac cgaaggtgca aaattagcag taggtaagcc cataaaagta gattacgtag
     4381 gctacgtaaa gataaaagca ctcggattta ttccggtaaa agttaaaata aacgacagtg
     4441 cctacttcac aaggaaagat gtcattggct attaatgtca ttcgtttttt atttctttga
     4501 tgtgtatcat tccattacta taaaaaagta actacctttg caaaaaaata aaaatgggtt
     4561 cgttaaaatc aatagtgctt tgtaaatgtc cgaaatgttc cgagggaaaa atgtttaaag
     4621 acagcaatcc ttaccatctt tccaagttga cagaaatggt agacgagtgt cctaattgcg
     4681 gatttaattt gaaaaacgaa accggatttc attttttgtc catgtatatg agctatatta
     4741 ttagttgtgc catttgcgga atcgttatta ttcccggcac ttatctggta ggtttggata
     4801 attttcctta tgtactcatt tttaacgcaa tattcttaat cttgttaatg ccattgattt
     4861 ttcggtgggc aaggatgctc agcttttggg caacatcaaa atttaagttt gattaaacta
     4921 cagatttttt tcagtaaacc tgtactattt acttcaaaat cttccggatt tcattggcct
     4981 catccataaa tgcttttaac tcttcatact tgttcttgtt gatgagattt ctgaaatgat
     5041 acatcttttc gatataagta tccattactt cgagtaagta atttttattc tgttgaaata
     5101 taggtgccca catgtccggc gagcttttgg ctaatcgtac agtgctttca aaaccaccgc
     5161 ctgccatttc gagtatggct tcttcatctt tctctttttc caataccgta acagccaaaa
     5221 cgaaggaact gatatgtgag atatgcgata cataagccgc atgcatatcg tgctgatgtg
     5281 cctccatata ttttatgcgc atacctaatg tgtcatacat actttctact aattttagtg
     5341 catcttctgc agtattttcc ttgtcgcaga taatacacac tttatttcta tataaattgg
     5401 gaatcgcggc tgatggaccg gagttttctg ttccggccat cggatgtgaa gccacatatc
     5461 gcatgcgcat gggatggtct ttgatggcat caataatatg tccttttgta gaacccatat
     5521 ccgttacggt ttgtcctgca cgtatattat ccaatacaaa ggttagctcg tttatcaata
     5581 tatttaccgg aacagtcagg atgattaaat cagctttctt aatggcttct tcgcggggca
     5641 aaatttcatc aaccaaccca agctccaatg ccttatcgca tgatgcttta cttttgccaa
     5701 ccccaataaa atgggtgcca aaatttgcct tccgcaaatc aatagatgca gaacctccaa
     5761 tcaatccgat accaaaaatt gcaatattca cagtgataaa attatgaatt cattttaatg
     5821 ataaaaaaag atacttatcc gcttactaac tttctgacat gtaaggatag gtataatatt
     5881 ttggtggggt atagctttct tttaccgtgc gtggtgttac ccagcgcaat aaattccatt
     5941 tgctgcctgc tttgtcattc gttcctgatt ttcgtgaacc accaaatggt tgttggccta
     6001 ccacagcacc tgtacattta tcattgatgt aaaaattacc tgcactattc accaaggctt
     6061 tctgcatttg ctcaatagca tatacatctt gcgcaattat agcacccgtc agtgcatatt
     6121 ctgaagtttc atctaccagt tttaacacat cttccagtct ggtttccttg tcatcataca
     6181 cgtaaatagt caatacagga ccgaaaattt cttctaccat ggttacagat ttcgggtttt
     6241 ttgtaagaat aacagtaggt tgaatgaaat accctttctg attttcatat tccccgccaa
     6301 acatgatgat attatccgga ttttctttag cctgttctat ataattcgtg atgcgtttgt
     6361 aggcatccgc actgataaca gctcccatga taacagaaaa atcatctgtc ttaccgattt
     6421 taaatgtctt taatgcttca tacatttttt cccaggtagt ttcccataat gatttgggga
     6481 tataagctct ggaacatgcc gaacattttt gtccctgata ttcaaaagca cctcgcagaa
     6541 tggcagttgc caatacatca gtgtctgcac ttggatgtgc caggataaaa tctttaccac
     6601 ctgtttctcc aactatgcga gggtaatttt tatacgtatc taaattttca ccgattgttt
     6661 tccaaataga attaaatacg gctgtagaac ccgtgtaatg tacgcctgca aaatcaggat
     6721 gtgttaaaat agcttgggta gtttcaacag gatccgtgta aatcatattg attactccat
     6781 ccggaagtcc ggctttgcgg aaaatttcca taatcgtcca ggctgaatat acctgataat
     6841 gtgaaggttt ccataccacc acatttccca ttaatgccgg agaggcacaa agatttccgg
     6901 ctatagccgt aaaattaaac ggggtaatag cataaataaa tccctctaac gggcgatact
     6961 ctatggtatt gcgtatacct ggtgcggata taggttgctc actataaatc tcagaagcga
     7021 aacgaacatt aaaacggaga aaatctgcca actcacatgc agcatctatt tctgcctgaa
     7081 atacattctt ggattgcccc agcatggtgg ctgcattgat agacgcacgg tatggccctg
     7141 aaatcaaatc ggcagctttc agaaaaatgg cggctctgtc ttcccaatgc attgcaaccc
     7201 attttgttct tgcatttagt gcagtattga ttgccaaatg cacatgttct aattttcctt
     7261 tatgatattg tccaagttgg tgcgccaact catgtggtgg gaatatggct gaagtttcat
     7321 ctgtacgtat ttctttgcca ttgattagca tcgggatatc atattgcgta tgttttctgc
     7381 ggtgaatttc tgcttttaag tccgcacgtt ctttgctgcc gggcatataa cctaagatag
     7441 gctcgttttg cggcattggg atgggggcaa ttttatttgg cataatcaaa aaaatttatg
     7501 caaaaatacg agttagaatt gatattgaag ttaaagatga taaagggttt tatttgtaat
     7561 aaataaaaaa tgtatgcgaa atggtgcaac ctaaaaagca gctaattttt tagctgcctc
     7621 ccacaataca atacccgcac ttacagaaat attaaaagaa tgtttagtgc ccatttgcgg
     7681 gatttctaca cattgcaagc aattttccat aaatacatcc gaaacgccat ctacttcatt
     7741 gccaaaaacc aaggcatatt tttttccaat ctgccaaata aaatcttgta aatagacaga
     7801 atcatcactt tgctcaatgg caatcaaatc aacttcgtat ttgtcaagtt cattaataca
     7861 tgcttcagct gtcgggaaat atttccagtc cacggtttca gttgcgccta aggcagtttt
     7921 taggatttcc ctgttaggag gagttgccgt tatgcctacc aaataaatac tttgaatgag
     7981 aaatgcatct gccgaacgaa aaacagagcc gatattcatg gcactgcgta cattgtctaa
     8041 aacaacaatt atcggcaatt tttcagcctg tttaaatgca tctttgctga ttcgatttag
     8101 ctcgtctagt ttaagtttgc gcataattat tgactgtcta tcaatccatc tggaagctgc
     8161 aaactgatgg atttcttttt attgtctatt ttaataataa aatcctcatt caatggtatc
     8221 agaatttctt tttcctgata tgtaacttgg gcaagaagtt gttgaggtgt ttcaaaaata
     8281 tcttccattt tacccaactt accaagcttt ttatcgatta aaatataacc gataagttca
     8341 tcatatcgtt cttcattaaa aacttcttct aactcttcct ctcgtacgaa taactctttg
     8401 ccacaaattt tttgtgcttt ttctttcgtg tcaatttctt ccaaaagcac gatagcattt
     8461 ccggggctgt ttaaatcttt agacaaaata aaatagggca aattctggat gaataaagct
     8521 tttattgttt gctcaaaatc tgtttgataa taggcatcaa aagcaatgcg gacatgccct
     8581 ttgaagccat gagtctttag tacttttcca atttttacaa gttccataca aaaaaattcc
     8641 cgacactaag gtcgggatta ttttactttt tcagtatttt attctgcatt ttcaggagtt
     8701 gcatcgcctg tgttttccac tgcatctgta gcctcagcaa ctgtttctgt actttcagct
     8761 gtttcaattg tttcttcaac aggaagttct tttactttag ccggagcttt tacagaggca
     8821 tcaattttag cttgttttac atcagcagct tttttctgat gatctattac cttatttacg
     8881 tgtgcatcta cccaagcagc aaattttgtt tctacatcat ctgcctgaac gatgcctaat
     8941 tttacaccgc gcaacaaatg ttttttgaaa aacgcacctt tgtaacgcaa aattgctttt
     9001 gcagtaggtg tcggttctgc gccgttttgc aaccattcta ccgctttgtt tacatccagt
     9061 tggatagaag ccggaatagt agttgggttg taagtgccta acaattcgat aaatttaccg
     9121 tctcttttta cacgcttgtt ggctaccacg attttgtaaa aaggtgcgcc cttagcgcca
     9181 tgtctttgta atctgattct tgttgccata ttaaaatatg tttttaagtt ttatatcctt
     9241 ttgactaaaa atagtcattc gcatttttaa atgaggtgca aaggtaatga aattcaatta
     9301 aaatactagt atttgtgtga tgattttttc atgcagttca caaaaaatca atttaacata
     9361 aatggagaaa atatctaaga gcaattcttg aagtttgtaa gcttattgtt aatcaatatc
     9421 aatcatttga tagaagattg atagattatt atctttaaaa gaatagactt ttctggtaat
     9481 ggcatcaatt aggcaacatt tttatgatca cttgctatta ctatgattta catttgtgtt
     9541 aagtgagtta ttttatgtca aaaggtttgt gattttataa taaattcgta aaattgattt
     9601 gcaaaacagt tgagggagtg gttcattttg gcaaaatcaa cgaaataagt aaattttgcg
     9661 aacaagtagt tttaatttta aatcaatttg ctttctaacc ttccattgag gatcgaaata
     9721 aaatgtaact aagcttaatg agaatttcaa gtaaaatatt agaggtaaac agacaaaaac
     9781 tactacttgc atttccaata gatttaaagc aagatgttga aatagtatca gagtttttgt
     9841 tagataaaaa tttaaaaatt gattcaatat cagaacaaga tatactattg aatggtcaaa
     9901 aattaataat accaagacga gtttatttcg acatttcaag tgtacaattt aataatactt
     9961 tgactaacac acaacaaaca attctaaact gtatttattt aagacatcat aatggttttg
    10021 tcagacaaga aggattagaa aaattaatta gtaatgcaga agactactat ataattcctt
    10081 atatatttca aattttagga gaatatgttg tggcactttt agagattgca gataaacaca
    10141 ttaatgaaaa tacaattgac aattacttga aattttgtat tgaaaatcca aaatatagaa
    10201 aactaacaga aagtagaatg ataagttact gggatgcata ttacagacgg acaaaattca
    10261 aaaacctaaa tgattattta ggtcgacaaa tttttaataa attataaata tagggataca
    10321 aatttaatta ttgtctctgg tttttcatca tctctaattt ataatttaaa atccataatt
    10381 tcacacctcc aagtggcagg catttatatt catatccctt tttgtaaaca ggcttgttcc
    10441 tattgcaatt ttcatttttc tacgcttact gtccaccgcg atgaaatgat aaaagcgata
    10501 ctcgctgaaa tagaagtgcg agctcatttt ttttctgata atacccaact gcaatccatt
    10561 tattttggtg gtggtacgcc cagtatcttg cctgttgaag ctattcaaat tatcttagaa
    10621 aaaatttatg ctactttttc cataactgaa aatcctgaaa taacactcga agccaatccg
    10681 gatgatttaa gcagagcgta tcttcagcaa ctaaaaactg aaaccaaaat caacagactc
    10741 agtatcggtg tgcaatcttt ttttgaagaa gatttgcact atatgcaccg tgcgcatcat
    10801 gctttgcaag ctgaaacggc tattaaaaat gctctggatt tcggtttttc taatctgaca
    10861 gtagatttga tttacggcgt tccaacttta agtgatgaac actggaaaaa aaacatacag
    10921 ctcttgctgg atttcggcat tccgcatatt tctgcttatg cacttacggt agaagataaa
    10981 acgatgctga aaagacatat acagcaacac aaaaaaattg cacctgagga tgataaagct
    11041 atccgccagt ataaactact gatagagcaa ttggaacatg ccggtttctt gcattacgaa
    11101 atttccaact ttgccaaact tggcttttta gccgtacata attcgtctta ttgggataat
    11161 acaccctatt taggtgttgg gccttcggca cacagttatg acggtaaaaa tcgcttttgg
    11221 aatattgcca ataatgccca atatatgaaa gcaatagcat cggggaattt gttgtttgag
    11281 aaggaagtat tatccgaaca agattttttc aatgaatata tgatgacaaa actgcgtact
    11341 caagctgggg tttctacaat agacttgcaa gatcgttttg gcaatatgat gaccttacat
    11401 tttttagaaa atattcaacc ttatgtacaa agaaatttag ttcaagttgc tcagcacaat
    11461 tatagattaa ctacggaagg aaaattatta tccgataaaa ttatttccga tttatttttg
    11521 gtgtaattac aaaaacaaat acactaaata gagcaacagt actaccaaca ataatttagc
    11581 ttttgcttta gtaatcgtta gtttttttcc cacccacaat aagagtaaga gcaaaatggt
    11641 aatgcacaag aacaagagta catcgaaatt aaatacttta ttataagcaa aaggcataat
    11701 gagcgcagcg gcacctgtca tgcccaataa attcagcata tccaagccaa tcaaattgct
    11761 gagttcaaat ttggatgaat gcgattttgt agaaataatc caataaagca tgacaaaaga
    11821 acctcctaca gataaaatac cctgccctaa aaacttctgc gagatatgat atttttgaga
    11881 gatgtctatc agattattga cgcataaaac tccaccggca accaacccta agatgcccac
    11941 taaaattaaa ataatggcaa accaaaccgg ataaaaatgt tgttccggct gttcatctgt
    12001 aaaccaaatg gtgtgatgtt tttttatatt gaaatagcca tacgtcataa acaacacaaa
    12061 tccagctaat agaatatatc cgtcagtacg ggaaaaattt attcctgaac ggtttgaaat
    12121 caaaacatca ttagccaaaa aataaaacag cactgtacta aataaaaggg tagcaatacc
    12181 gatagtagcc gtagtaagat gtgtgcgcat agggcgggtt agtcctacga ttgcaagtac
    12241 gccaattaga ttaaagatac aactgcccag aatagtaccg attactaaat ccgtgtagcc
    12301 gttgtatgca gacagtaata tggtaaataa ttttggaagg gaagtaccaa aagcaattgc
    12361 cgtgaaatta atggctgttt cgggtactga aattttgcgc aaaatagaag tgcttccatc
    12421 gatgagcata cctgcccctc tgttgagcaa gaaaaacccg atcaacaaca aaggaatatt
    12481 taagaaaagg gataaatgtt tgaattccac gcgctgggta agttggttga ttagtgattg
    12541 gttaattgct gataggtata ttgattactg gtgctatgga tttggtgatt atcaaaaaaa
    12601 taaaatacaa tttgcatagg tgaaataatc agctatcata attctatctt ttttttcttc
    12661 ttataattct tgttttgtac caattattat acacatcatc tgaatcatgt actggttgaa
    12721 tgcacactct gcctattaaa tgtcttgcaa taattggttt cgtcttattt atattgcttt
    12781 cataaatatt tgttttaata gtgtccgtat ctacattttt tgttgagatg ggttctgatt
    12841 tctcaataac aacttttgga ttcttatcaa tactatcttg tgcatggcta agttgcgtac
    12901 tcattaatcc caaagctaat ccggctgcat atttaaactt gctgtgtctg tatggaggat
    12961 aaatgacata cgtttcttcg agttgtcttt tcaaaaaacg accacatgtt ttttcttttt
    13021 tttgcgaaaa aaatgcagca atttcggaag aagttttatc agaaaaatca attacttctt
    13081 tttgacaaac agcacaaaat gcaccttgtt ccgtctgtga catatctgcc cagttctcca
    13141 aacagggttc atcaatgctt aaacgaaaag tatgtttttt catttcaagt atttgaatta
    13201 ataattacta acaattccaa aatcagtatc aaaaaagctg ttgtcaattt ctaaattaat
    13261 ttttcctgat attatgttag aataattttt ataggcttga gataatttaa tatattttgt
    13321 ttgataacct ccaaaagaaa agcaaaaaat agctggaact gaatcaatag taagcgtaaa
    13381 tttgccatta atatctgaga ctgttcccat agtcgaatta aatagggtta catttacacc
    13441 tactaaggct tctccgtttt catctacaac ataacctgat atagtataaa aatctttttt
    13501 tgattttgtt tcaattaaac tatcctgaac ttgagtatga catgatacaa cttgaatttt
    13561 ttcaaaattt gtaagcttgt ttttagcact gcttgagttt gtaaataata atcccaaagc
    13621 caatcctgct gcatatttaa atttgctttt tctgtacgga ggataaataa cataggtttc
    13681 ttcgagttgg cttttcaaaa aacgaccgca tgttttttct tttttctgtg aaaaaaatgc
    13741 agcaatttcg gaagaagttt tatcagaaaa atcaattact tctttttgac aaacagcaca
    13801 aaatgcacct tgttccgtct gtgacatatc tgcccagttc tccaaacagg gttcatcaat
    13861 gctcaaacga aaggtgtgtt ttttcatacg tatgctatat tagtattaca aaaagaattg
    13921 ctggattttt aaatcacctc atctccacat ttactcatct tacatctcca catttatttg
    13981 tgtgattgca gtagctcatc caccaaatcg atatagcttt gcatggcttc atcactgctc
    14041 atgccggctt tgtttttcca tgcatcccat ttgaatttac ctttgatatc aaacataccc
    14101 ggcttatctg tatcattatc gccttcggtg gcttgtttga aaaatgcata taaatcgagc
    14161 agttgcatat cactcggacg tttggttaat gtttttacct ctttttgtgc ttgttcaaat
    14221 ttttcctgaa tactcatatt ttaaaattta taccctaaat ttatgaaatg atttcgagaa
    14281 agtttcaatt tacttttctt atctgcattc ctctgttgtg gttttttttc aaactgcttc
    14341 cggtctacac tttgcccgca gattgctcca tgcctaattt tatttatcct gaaaatatag
    14401 agcatcttaa aatctggtgc tttgacaaac attcagatat ccgctttgac gacagcaata
    14461 attttctatt tatcagtttg atgtgggtgg gtatgcattt tctgaaactg actgctatta
    14521 aagcttccct ttggataagc ggaatcagtg tattgcttac cgtatggctg atgcagcgta
    14581 cggtagatac aagattctcc tcaatacaat tgcttttagt aggatttgta tttttatctg
    14641 ttcaggtttg gacgggtgta ctcggtgatg aaatattatt cagtggtatg ctttggcttt
    14701 tcgttataca tgccttctgg aaacatcgtt attttggtat tatggtttgg ggaacaattg
    14761 ccattattgc gcaagtacaa aatattcttc ttgttttacc actcctaatt gccagctatt
    14821 gggatatcaa acaatataaa gaacgcgacc gaaagcgatt ttggcgtcgc cggataagtg
    14881 aaactgtctt gttttttatg ctgccgctcg ggctctatta tttttaccga tttgcctatt
    14941 tcgggaaagt tttgcctttc gattggtatc atcatcagtt agaaagcgac cgaaaatact
    15001 ggatatttaa tgttgaagca tggaatgtat tgaaacacta tgtgcgttat tttattttgc
    15061 cattggtgtt gggtatttta ttttattttg taaaacaacg caaaaaactg aatcataaat
    15121 actacgcatt gttttatgcc atgatacttt tcccgatgct ttacaatctg acgatatcgc
    15181 aagatgaaaa tttgggatac aagaactttt attcgcttta tttgggatta tcacttttgt
    15241 tattgttgtt tctgagagat tttcgttcca tttctatggg gttttttgcc tttatttttg
    15301 tgttttttta tgggtttaag cagtcatttc attcatttgg caaagctttg caatcgcata
    15361 atcagaatat gtattatttg tctaccgatt tggcagaaat acacgatggc agtgcaatcg
    15421 tatattacga taatttttta ggatggaata ccgactggaa gatatatttt gcaaatggca
    15481 aacacagcaa agtaaaagaa gaaatgacgc tcgaaaatat gtttaattcc gatgcggaca
    15541 tgttgttttt aaaagagccg attttagatt atcccgaaaa taaatttgtc ggatattcca
    15601 ttccggtcaa tacaatgcaa tatgtcaaag aggctgaacc tgaaaattca ttagataaat
    15661 ttttctataa atacgagcat aaagtgccgt tagatacacg tatgataccg atattgctca
    15721 ataaaagaag caagcgtttt gatgccatta agaaagctat agaaaacaga ggagcggtat
    15781 tgaataacta gcgtatgcca aatcctgcct tgatatccgc ttgtacaaaa tgcaatttgc
    15841 cattttcatc ttctgccatt aaaatcatgc cattgctttc tatgcctctc agtttgcgag
    15901 gtgctaaatt aaccaatacc gaaacttttt gtccgatgat attttcaggt gcaaaatgct
    15961 cagcaatacc agatactatc gtgcgttgct cgatgccgat atccaccaag agttttaata
    16021 atttatcggc tttaggcact ttctcggctt ctaaaatgat gccggtgcgg atatccattt
    16081 ttgcaaaatc atcaaattga atttcagttt tcagttctgt ataagtggta ttggcagcct
    16141 cactttgtgt ttcatcttgg ctcttgattc ttgactcttt aatcttgttt aatttctcaa
    16201 tttgaaatgc aatttgttca tcttcgattt ttggaaaaag cagtttcgct tcatttattt
    16261 tatccgattt cagttggtaa ttaaacggat ttgcatcttg cacatcggca ttcagcatgt
    16321 cgaaaatttt atgagcagtt tttggcaata aaggttgaga aaatacggcc aaagcataca
    16381 cccaatcaag acttaacgct aaaatatctt tcgttttttc agggttttct ttaattaatt
    16441 tccatggctc ggtatcggct aaaaatttgt ttccactgcg ggcaaatgcc attaactcat
    16501 tcagcgcatc tttaaaacga aattgttcga tagaagatgc aatagcaagt tctgtttcta
    16561 taattgtttt gctcaaaata gaatcatcat cgcgcatcgt tttgcgaaaa ctgtcatcga
    16621 gtttaccgtc ataatattta tgaataagcg taaccacccg attgacgaaa ttgccataag
    16681 ctgctaccaa ttcattattc actctgtcct gataatcttt ccaggtaaac tcgctgtctt
    16741 tattttcggg catattgctg gtgagtacat agcgcaattc atcttccttg ccggggaaat
    16801 cttgtaaata ctcatgcagc caaactgccc aatttctgga tgtagatatt ttatcgcctt
    16861 ccaaattcat aaattcatta gcaggtacat tgtcgggcaa gatatattgc tgtgtagctt
    16921 gtaatattgc cggaaacgta atacaatgaa aaacgatatt gtctttaccg ataaaatgaa
    16981 tcaatgtggt ttcatcatct tgccagtatt ttttccagtc ttcagctttc ttgtttttta
    17041 ttttctccgc aaaattttcg ggataagcaa attcaaattt tccactttct atttctctga
    17101 ataattgttt gctggcagaa atatagccaa tgggtgcatc taaccaaacg tataatactt
    17161 taccttcact gccttctaat tcgggcggaa caggaatacc ccaatccaaa tcgcgtgtca
    17221 tggctctggg tttcaagccg tcattcaacc acgatttaca ttgaccaacc acatgcgctt
    17281 tccagttatt ttcagccgcc gttttttcca gccagttttt tatatcttct tgctgttcgt
    17341 ctaattttaa aaaccagtgt ttggtttttc tcagcaccgg cgcgctgccg ctgatggt
//
LOCUS       DAOQII010000017        17163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1584_length_17163_cov_5.824877, whole genome shotgun sequence.
ACCESSION   DAOQII010000017 DAOQII010000000
VERSION     DAOQII010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1584_length_17163_cov_5.824877"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLF48_01705"
     CDS             complement(<1..304)
                     /locus_tag="PLF48_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48065.1"
                     /translation="MNSNINFNELWKGQQADQPTTQELQKKTRKLKYTGLLRMLFITL
                     TFVATALFVCWIWYKYKPEMLTTKIGMILTLLALFIYGISFQLQFPLLIKLDSTENN"
     gene            complement(294..764)
                     /locus_tag="PLF48_01710"
     CDS             complement(294..764)
                     /locus_tag="PLF48_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF48066.1"
                     /translation="MDFEAIYKNYWQQIFRLCMGYTNDYEMARDMAQETFIAVWKQLP
                     GFRHEASIGTWIYRIATNLCLRHIEKVKRYPIAEFPEHLQEENYYNLEPNIKLLYQFI
                     AELPEIDRIIISLELENIKQTEIADITGMTEANIRVRIHRIKEKLTQKFKQNEQ"
     gene            complement(766..1617)
                     /locus_tag="PLF48_01715"
     CDS             complement(766..1617)
                     /locus_tag="PLF48_01715"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48067.1"
                     /translation="MNKLKINFVIVLLLFLLNNSFAQNPDTAMQVIKSGKGQKSIIFI
                     PGFACSGEVWNETKTNLEQNFVCYTLTMPGFASAKPQFNPSFKTWEKNIADFIKKNNI
                     VKPIVFGHSMGGGLALALASDYPDLIEKIIVVDALPCLAALSNPAFKQETNPDCSAMT
                     DRLVNATQEQFYAMQKATIFRLVADTSMQQQVLNWSKQSDRNTMAKMYCDFTNTDLRE
                     SIAKVTCPALVLLQSYFINFKPAIDEQYKNLCTAKLQYATKGLHFIMYDDKAWYFNQI
                     SNFISVN"
     gene            complement(1752..2693)
                     /locus_tag="PLF48_01720"
     CDS             complement(1752..2693)
                     /locus_tag="PLF48_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF48068.1"
                     /translation="MNISVVIPLYNEAESLPELFAWIKKMMLEHNYTYEIIAVNDGST
                     DASWEVICKEAQTNSNIKGISFQRNYGKSAALHVGFQQAKGDVVITMDADLQDSPEEI
                     PELYNMITQQDYDLVSGWKKKRYDPISKTIPTKLFNAATRKVSGIDNLHDFNCGLKAY
                     KNKVVKSIEVYGEMHRYIPVMAKWAGYSKIGEKAVQHQERKYGVTKFGLERFVNGFLD
                     LMSITFVGRYGKRPMHLFGTLGVLSFFIGFVILLYLSISKLFFSQIGIATRPLFYLGI
                     MVIIIGVQLFLAGFIGELISRNGIEKNHYNIGDRVNL"
     gene            complement(2695..3132)
                     /locus_tag="PLF48_01725"
     CDS             complement(2695..3132)
                     /locus_tag="PLF48_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48069.1"
                     /translation="MENNEQDYSPRGVLSGITIALFSAFVSVLFFAAFYSLAYVKKMP
                     FGNMQAVILQTTAILTTVVMYQYLSRKIQGNKLSIMQAFLGGWMASLVLALFVITFYS
                     IFQKITKINMIPDGSGVFSSVLMLYSAIGMFISLIFALILKKS"
     gene            complement(3122..4390)
                     /locus_tag="PLF48_01730"
     CDS             complement(3122..4390)
                     /locus_tag="PLF48_01730"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF48070.1"
                     /translation="MKHLLKNAILLQPGHDFHDKQADLLIEDGIIKKIASSISDSDAQ
                     VYDFNGCHIAPAFCDLYVSICDPGFEYRDDINTITQSALAGGYTAICATADNSPITQT
                     KAQVEYIINKAKNSGIAILPVGAVTENFDGKSPTEMLDMHHAGAIAFSDMPNSIKNSG
                     VMLRALQYVQPFNGLIITMPFDATLVGDGQVNEGEISVRMGMYGISNVSEYSALQRDI
                     EILKYTGGKLHIAGISTKESLDLIRKAKEEKLNITCSVFVHHLLLTEEEVSGYDSHYK
                     VFPPLRTEEDRNTLIIGLADGTIDCISTQHIPLDIDSKNLEFEYALNGIIGLESALGL
                     LHKHLGKHLSQEKLIELLAVNPRKILGLDYQIKTGKQADFILIDFDKDWKFADKDIRS
                     KSKNTPFIGSELKGIVKAVFSKDKVFLNGK"
     gene            complement(4400..6439)
                     /locus_tag="PLF48_01735"
     CDS             complement(4400..6439)
                     /locus_tag="PLF48_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNF48071.1"
                     /translation="MHFVFPWFLLALAALAVPVIIHLFHFRRYKKIYFSDIRFLKQVN
                     EEKTTIDELKNRLILASRLFALFFLVLAFAQPFLGDKKSQLNKGASAVAVYIDNSYSM
                     GLKKAGESGLDMAKSKAKEIANAFNTGDKFALLTNDLEGSHQRWMQKQDFIDAVDKVE
                     LTSAVKTTNEIAQKESALFQSAGMKNKLGFFISDFQKNMLANLSDTNYTAYFLPLTSD
                     EEKNIFVDSCWMEQPLFTLNAVNKMLVRLVNSGNSNVEKLRISIKINGQVKAINDVDI
                     PAGNTRVDSFSFTITQPGIQRGEIELNDYPITFDDHFYFAFTIQENEKVLSIEDAASG
                     NYIASIFSGDKHFAFDKVNKGQVDYSTLKNYSLIILNQLSDISSGLNSTLKDFVNNGG
                     NIYVIPAGNANLASYNAFLTAVNAGILGAFQTKNGEVDKINLKEDIFKSVFSALPKNV
                     ETPKIKQFYPILSRSSLQERQLLSTNLGSPLVTRFDAGNGFVYVQAVPLNPDFSNLQA
                     TAIFAPMVYNFAVYKKNTTPLYYTIGKDNLIELKNEQTSGEQIYKLTNGKNEFIPENR
                     SIGNTLLLHVNQILPSDGHYTISNNEKMLGVAAFNFDRSESVLKFAKASELKQKYTLK
                     NQLVLDNSAVNLSAKVKQIKDGVLFWKVCVILALLFLLAEILLIRFWKQASSAAN"
     gene            complement(6553..7074)
                     /locus_tag="PLF48_01740"
     CDS             complement(6553..7074)
                     /locus_tag="PLF48_01740"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HNF48072.1"
                     /translation="MNAYSSQIPIFIKKLLMENQELLDNNLNLTNELSLTSSAKDFLL
                     QTAKWAKFLSIIGFVFIGLIVIAALFIGTFMGKLNTVADTGMGSGQSIFLTIYFIVIG
                     LITIYPVLRLFQFSQKAKLAVETNNTLALEESFKRIRSVFRFYGIMMIIVLSIYAIAI
                     IFALVVGSSGIFN"
     gene            7134..8312
                     /locus_tag="PLF48_01745"
     CDS             7134..8312
                     /locus_tag="PLF48_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNF48073.1"
                     /translation="MKLTFPDNFIWGTSTAAYQIETAGEHNWKGFKSKDGSIFDKCSQ
                     HELHREEDLNYICQMGNAYRMSHDWSKLQKAPFAEFDKTETEAYLKFMQELKNRGIHI
                     MLVLHHFTNPLWFEEAGSWEKEGNRKMWLDFVQKTVNTFGHLVDSWNTFNEPNVYVSN
                     GWITGFFPPFKKNKLLLARKVLKEMSKAHEEAYDIIKQESNAPIGISFNTVKFAGEVF
                     PGHIAAKISDWWFMEYCHNHFLKVDFQGLSYYARIPYRPHPIDNINTPGVLDKLGRKQ
                     DLMWEYAPEEFYHIFHRYWKKTKKPIIITESGVCTYDAAFRIQSIKDYLGWMKKAMDD
                     GIDIRGYFHWSTIDNHEWNIGTYYKFGLVSVDFETGKRTMTKAGEFLGDIAKNNSVEV
                     "
     gene            8892..10757
                     /gene="mutL"
                     /locus_tag="PLF48_01750"
     CDS             8892..10757
                     /gene="mutL"
                     /locus_tag="PLF48_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNF48074.1"
                     /translation="MSDIIRLLPDHIANQIAAGEVIQRPASVVKELMENSIDAGAQHI
                     QLIIKDSGRTLIQVVDDGKGMSETDARMSFERHATSKINKIEDLFSLHTFGFRGEAMA
                     SIAAVAHVEMKTKTADRENGVILIIEGSKVVLQEPCSHGNGTSISVKNLFYNVPARRN
                     FLKSNQIEFKHILDEFIHVALANPQIFFSMHHNGLEIYHLKSGNLRQRIVGILGKNYN
                     DKLVPIEELTEYVSVRGFVGKPELSRKTRGEQYFFVNNRFIRNNFLNHAVAKAYDGLI
                     HDKSYPFYCLFIDINPDAIDVNVHPTKQEIKFEDEKSVYLLLNAATKHALSQYSIAPT
                     IDFEHEKMFNTLVDEKVHFEQPFKVLPSKANTNVSSKGSYDSPFHKDIPTHEQTYEQW
                     KKLYETDEETEEKVLTIRSKMDQLEALENENEISNRPFKDERFEPIQLHFRYILTQIK
                     SGFILVDQYKAHQRILFEKYLRNLSQTASASQQTLFPKSLELNPSDTIILQEILPELK
                     ILGFDIDFFGKTTFVIHGMPGDLADENETLLIEKLLEEYKNSGKGETIDKRNNVAKTL
                     AYQTAIKPGKLLSKMEMQTLIDELFACEQPLLNPAGLATFVQFTFDEIEHKFNAV"
     gene            10754..11503
                     /locus_tag="PLF48_01755"
     CDS             10754..11503
                     /locus_tag="PLF48_01755"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF48075.1"
                     /translation="MSNFRRTGGFMQMTPLVKNLIIINVAIFLVDYLIFPQLNSYLAV
                     YYPSNPDFKPYQLVTHMFAHAGFAHIFFNMFALWNFGCIVEQMLGIKRFFLLYFISGF
                     GAIAAHLLINGLMIYHHIGTFNLQPDAFINTEGLKLLSTNPDANWLPYVSNVFLQNQY
                     ISVMLGASGAINGIVAAFAYLFPNTELILLFFPVPIKAKYAIPGILILYDLGYGSFAN
                     TGVAHYAHIGGAITGFLLVYFWNKTNKTTFY"
     gene            11537..12433
                     /locus_tag="PLF48_01760"
     CDS             11537..12433
                     /locus_tag="PLF48_01760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF48076.1"
                     /translation="MNGKSIFEDIKWQYIYGSNLIRLILINVAVFVVLGLINLPFFFS
                     QSDFNVYSFFYLYSNPSELMRHPWGFITYMFVHQSFFHLLFNMLGMYWFGMIVEDLVG
                     KSKIVPLYLYGGLFGGILFVLSYNFFDALSVGYYSAIALGASASVLAFVVAGAVIAPD
                     YELNLFMIWRVKLKWIALAYVVLDLINIPNGNAGGHIAHIGGAIMGFVYIRLLQNGRD
                     LAKPYYKLEDFFVHLFRKKSSEMKVNKNTSSNYDKSSTIKTSHQDKQVRLDAILDKIN
                     KSSYESLSKEEKDFLFQLSKED"
     gene            12430..13548
                     /locus_tag="PLF48_01765"
     CDS             12430..13548
                     /locus_tag="PLF48_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF48077.1"
                     /translation="MKIIKWLISFLSRTTNIIAVAALLLCLSAAYLHPEKVWMLPLFG
                     MLLPFFIFVNLLFVFIWIWRRRYFALVSAFAIMLCVPQLLHLFSFGIFKNHEVADKPK
                     IKILSYNVRDFDLYNWSENRNSKNEIFKTIKDIDADIICFQEFYSDTSQTFNTIKQLQ
                     EAGYSHYYFTRELVLRNTDEWGIAIFSKKQIVDSGQILKQNFKTGYGKQPYKGIYADF
                     AFSDTTIRIFCVHLQSIYFGKDEYQTIVELKENQNIDENGVMKIVGKLRRAFVRRAKQ
                     ATELKLILDKQTLPYIVCGDFNDLPNSYVYNTVSSNLKDAFLENGFGVGSTYSGKIPF
                     LRIDYMLSQPMLQPVAFRIDKNKISDHHPVWAEYVFEK"
     gene            complement(13550..14518)
                     /locus_tag="PLF48_01770"
     CDS             complement(13550..14518)
                     /locus_tag="PLF48_01770"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HNF48078.1"
                     /translation="MSIFSVFTVIAQEKINMQLQLKKGDSYRVTIPMNAPMQMETKLD
                     PEAMKKLLLNMGIEEDSVEAQIKSMEDDKEANQTIQMNMSMEMCLLFNVIETTSEKYT
                     LEAYYEYLETSVQAKDETYSISTRKKSEGLNEDQEKELNSLKNIIGKKFKVNIDSRGK
                     LLSLEGYDKILKAAKIKKSKDFLSNENSMLSDQLQTDEINTTMSGIFDILPPTEVKIG
                     STWTKDISVKDELTPYTIKTKYTLKEIRRDDVVILSESVFYNNLEDAKLVTVDGTGSG
                     NITMKRADYFQQEQPYNYELKMKMKLFGMNMNIVTPASGTYKVEKL"
     gene            complement(14623..15024)
                     /locus_tag="PLF48_01775"
     CDS             complement(14623..15024)
                     /locus_tag="PLF48_01775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF48079.1"
                     /translation="MVKIADLKISSRKENIVDVETLVESLRIQFELNDTKYHNMLLAS
                     VEAVNNAIEHGNNSDEDKNVYISAIRCETTLKLSIKDEGRGFNPDKLPDPTCGKFLEQ
                     PDGRGVFLIRQLADEVAFLDGGTRIEMKFRL"
     gene            complement(15210..16025)
                     /locus_tag="PLF48_01780"
     CDS             complement(15210..16025)
                     /locus_tag="PLF48_01780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF48080.1"
                     /translation="MKKQILHLILFLFSLNLLAQNNATTKSKPATTAQKKTTTKPVTK
                     KTTSKTGTKTSTTSKTKTTATKTNKMDTSTYVIITTDLGTMKLRLYDETPLHKANFIK
                     IVQDGVLDSTLFHRVIPQFMIQGGDVNSKHAQPGQQLGTGNLPYTVPAEFNPKLIHKK
                     GALAAARTGDFINPKKESSSTQFYIVQGKSYTPAELTMLGQRTGHGPWTEEQIKIYGQ
                     NGGTPMLDMQYTVFGEVVEGFEVIDKIAAAQRDGADRPFTDIRMQVKIEKNGK"
     gene            complement(16035..16385)
                     /locus_tag="PLF48_01785"
     CDS             complement(16035..16385)
                     /locus_tag="PLF48_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48081.1"
                     /translation="MQENKEQHPAGWENIEAHLSKVYGKVPDLPGILFLIGHRELGKH
                     QTKFSKEQKQDLIHVAVCTLLSAKGYYAFVALDEDGWPHFEPLKNAPKLEHNQQENLL
                     KEAIVDYFYREEIL"
     gene            complement(16388..>17163)
                     /locus_tag="PLF48_01790"
     CDS             complement(16388..>17163)
                     /locus_tag="PLF48_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNF48082.1"
                     /translation="FTGMFDNLHIALVQPDLAWKNKKLNLEHIEDMIFPLKQAADIFI
                     LPELFNTAFCVDDPELAETENGDTVSWMQQLASAKETVVCGSLLIKEEDKIYNRFLWV
                     DKNGIQYRYDKHHLFSLVNENESNLIYPGKNKISISYDKWEIQPFICYDLRFPAWCQN
                     DHNVQVQIFVASWPQKRIHHWMTLLQARAIENQCYTIGVNRIGEDGHGHLHNGHSCSF
                     DYKGDLLCDMQEKNGIEIVALSKEKLEKYRNQYPFWKDR"
BASE COUNT         5194 a         3181 c         3192 g         5596 t
ORIGIN      
        1 cattattttc tgtgctgtcc agttttatca gaagcggaaa ttgaagttga aagcttatgc
       61 cataaatgaa caaagcaagc aatgtaagta tcatgccgat ttttgtagtc agcatttcgg
      121 gtttatattt ataccaaatc caacagacga ataatgcagt agccacaaaa gtaagtgtga
      181 taaaaagcat tcgcagcaga ccggtatatt ttaatttacg tgtttttttc tgcaattcct
      241 gtgtagtagg ttggtcggct tgttgtcctt tccatagttc attaaagttt atattactgt
      301 tcattttgct taaatttttg ggttaatttt tctttaatac gatgaattct tactctgata
      361 ttagcttcag tcatccctgt aatatcggca atctctgttt gctttatatt ttccagttcc
      421 aatgaaatga taatacggtc aatttcggga agttcagcaa taaattgata gagtagttta
      481 atattcggtt caagattgta atagttttct tcttgtagat gttccggaaa ttctgctatt
      541 ggataacgct tgactttctc aatatgacgt aaacaaagat tagttgcaat gcgatatatc
      601 caagtgccga tactggcttc atgtctaaat ccgggtaact gtttccaaac ggctataaag
      661 gtttcttgtg ccatgtctct cgccatttca taatcattag tatagcccat acacaaacga
      721 aagatctgtt gccagtaatt tttatatatt gcttcaaagt ccatatcagt taacacttat
      781 aaaattactg atttgattaa aataccaagc cttatcgtca tacatgataa aatgtaatcc
      841 tttggttgca tattgcaact tagcagtaca cagattttta tattgctcat caatagcagg
      901 tttgaaattt ataaaataag attgcaataa tactaatgcc ggacatgtaa ctttggcaat
      961 ggattccctc aaatcagtat tagtgaaatc gcaatacatt tttgccattg tgtttctgtc
     1021 agattgctta ctccagttca atacctgttg ttgcatggat gtatctgcga caagtcgaaa
     1081 tattgtggct ttctgcatag cataaaattg ctcttgtgta gcatttacga gtctatctgt
     1141 catagcactg cagtcagggt ttgtttcttg cttaaaggca ggattgctaa gtgcggcaag
     1201 acagggcaac gcatctacaa caataatttt ttcaatcaaa tcgggatagt cggaagctag
     1261 tgccaatgct aatccaccgc ccatgctgtg accgaataca atcggtttta caatattatt
     1321 cttttttata aaatccgcaa tgtttttttc ccatgtttta aatgatggat taaattgtgg
     1381 tttagcactt gcaaatccgg gcatggtcag cgtataacac acaaaatttt gctctaagtt
     1441 agttttggtt tcattccaca cttcacctga gcaggcaaat ccggggatga aaataattga
     1501 tttttgtcct ttaccgcttt taattacttg cattgccgta tccggatttt gagcaaaact
     1561 gttattgagc aagaacaata gtagtacgat gacgaagttg atttttagct tattcatgtt
     1621 gtgtaattta attacccttt agatacacaa agactaaaaa tgttacaaga aagttgaaaa
     1681 aaattcttct gctaaataaa caaatatact ttgaaatatg ttattaaaca acaactgtta
     1741 ctttcggtga gctacagatt cactctatca ccgatattgt aatggttttt ttcaatgccg
     1801 ttgcgggaga ttagctcgcc gataaacccg gctaaaaaca actgcacccc gataatgatg
     1861 accatgatac ccagataaaa cagcggacga gtagcgatac cgatttgcga gaaaaataac
     1921 tttgagattg ataaatacaa tagaatgaca aaaccaataa agaatgacaa tacgcctaat
     1981 gtgccgaata aatgcatagg gcgtttgcca tagcggccaa caaacgtgat agacatcaga
     2041 tctaaaaaac cattgacaaa acgctctaaa ccaaactttg ttacgccata tttgcgctct
     2101 tgatgttgta ctgccttttc gccaattttt gaataccccg cccactttgc catcaccgga
     2161 atatagcggt gcatttctcc atatacttcg atgcttttta ccactttgtt tttataggct
     2221 ttcaacccgc agttaaaatc atgcaagttg tcaatcccgg aaactttgcg cgtagccgca
     2281 ttaaataatt ttgtcggtat agttttactt atagggtcgt agcgtttctt tttccatcct
     2341 gataccaaat cataatcctg ctgagtaatc atgttataca attccggtat ctcttccgga
     2401 gaatcttgca aatcggcatc catggtaatc accacgtcgc ctttagcctg ttgaaagcct
     2461 acatgcaagg ctgcagattt gccgtaattc cgctggaaag atataccttt tatgttggaa
     2521 ttcgtttgcg cttctttgca aatgacttcc caagaggcat ccgtactgcc atcgtttaca
     2581 gcaataattt cataggtgta attatgctcc agcatcattt tttttatcca ggcaaatagc
     2641 tccggcagcg actcggcctc gttgtataat ggtatgacta cagaaatatt caaatcagct
     2701 ttttttcaat atcagcgcaa agataagcga aataaacata ccaatggcgc tgtataacat
     2761 taataccgat gaaaatacac cactcccatc gggtatcata ttgatttttg ttattttctg
     2821 aaaaatggaa taaaaggtga taacgaataa tgccagtaca aggcttgcca tccatccgcc
     2881 caaaaatgcc tgcatgatgc tcagtttatt gccttgtatt ttgcgcgata aatactgata
     2941 catcaccacg gtagtcaata tggcagtcgt ctgcaaaatt acggcttgca tattgccaaa
     3001 aggcatcttc tttacatagg ctaaagaata aaaggcggca aaaaacaaga cggaaacaaa
     3061 tgcagaaaag agtgcgatgg taataccgct gagtacacct ctggggctat agtcctgttc
     3121 attattttcc attcaaaaac actttatctt ttgaaaaaac cgcttttaca attcctttta
     3181 attccgagcc gataaacggc gtattttttg atttggagcg aatgtcttta tctgcaaatt
     3241 tccagtcttt atcaaaatca atcaggataa aatctgcttg ttttcctgtt tttatctgat
     3301 aatccaatcc aagtattttt ctggggttta cagctaataa ttctatcagc ttttcttgtg
     3361 aaaggtgttt gcccaaatgt ttatgcaata agcccagcgc actttccagc ccgataatgc
     3421 cattcagtgc atattcaaat tccagatttt ttgagtctat atccaacggt atatgctgcg
     3481 tagaaataca gtctattgtg ccatctgcca acccaattat tagagtattt ctgtcttctt
     3541 ctgttcttaa tggaggaaat actttatagt gggaatcata gccgctgact tcctcttctg
     3601 tcagcaataa gtgatgtaca aaaaccgagc aagtgatatt cagtttttct tctttcgctt
     3661 ttctgattaa gtccagactt tctttggtag agatgcctgc tatgtgcaat ttacctcctg
     3721 tgtatttcag tatctcaata tcgcgctgca aagcactata ttccgatacg ttagaaatgc
     3781 catacatgcc cattcttaca gatatttcac cctcgtttac ttgtccatca cctaccaacg
     3841 tggcatcaaa aggcatagtt atgataagtc cgttaaacgg ctgcacatat tgcaatgctc
     3901 tgagcatgac accactgttt tttatactgt tcggcatatc gctaaatgct atagctcctg
     3961 catgatgcat atccagcatt tctgtcggag atttgccgtc aaaattttct gtaaccgcac
     4021 caacaggcaa aatagcaata cctgaatttt ttgctttatt gatgatatat tctacttgtg
     4081 ctttggtttg tgtaatggga gaattatcag ctgtggcaca aatggcagta tagcctccgg
     4141 ccagtgccga ctgagtaatg gtgttgatgt catcgcggta ttcaaatccg gggtcacaaa
     4201 tacttacata caaatcgcaa aaagcgggag caatatgaca accattaaaa tcatagactt
     4261 gagcatccga atcggaaatg gacgatgcta ttttttttat aatgccatct tcaatcaata
     4321 aatctgcttg cttatcatga aaatcatgtc cgggttgcag gagtatggca ttttttagta
     4381 ggtgtttcat ctttcaggtt cagtttgccg cagatgatgc ttgtttccaa aatcgtatca
     4441 acagaatttc tgctaacaga aataacagtg ccaaaattac gcaaactttc caaaataaaa
     4501 cgccgtcttt tatttgtttt acttttgccg aaagattcac tgcgctgtta tccaagacca
     4561 actgattctt taaagtatat ttttgtttca attcactagc tttggcaaat ttcaagaccg
     4621 attcgcttct gtcaaaatta aaggcagcta cacccagcat tttttcatta ttggaaatgg
     4681 tataatgccc gtcgctgggc aaaatctgat tgacatgtaa aagcagcgta ttgccaattg
     4741 atcggttttc agggataaat tcgtttttgc cattcgtaag tttataaatc tgctcgcccg
     4801 aagtttgctc atttttcaac tcaataagat tatctttccc tatggtatag tataaaggag
     4861 ttgtgttttt tttgtaaacg gcaaaattgt aaaccatagg ggcaaatatt gctgtagctt
     4921 gcaaattaga aaaatcagga ttaagcggaa ctgcctgaac ataaacaaat ccatttcctg
     4981 catcaaatct tgtaaccaat ggcgaaccta aattagtgct caataattgc cgttcttgca
     5041 aactgctgcg ggataaaatg ggataaaatt gcttgatttt gggtgtttct acatttttgg
     5101 gcaatgcact gaaaacactt ttaaaaatgt cttctttaag attgatcttg tcaacttctc
     5161 cgtttttagt ttgaaaagcc cccaagatac ctgcattaac ggcagtgaga aatgcattgt
     5221 aagatgcaag attagcattg ccggcaggga tgacataaat attgccgccg ttattgacaa
     5281 aatcttttaa agtggaattc aaaccggagc tgatatcgct cagttgattt agtataataa
     5341 gcgaatagtt ttttaatgta gaataatcaa cttgtccctt gttcaccttg tcgaaagcga
     5401 aatgcttgtc gcccgaaaaa atggacgcta tataattgcc cgatgctgca tcttcaatag
     5461 ataatacttt ttcattttcc tgaatcgtaa atgcaaaata aaaatgatcg tcaaacgtaa
     5521 tcggataatc attcagctca atttcgccac gttgtatgcc cggctgtgtg atggtaaagg
     5581 agaaggaatc tactctcgta tttccggccg gaatatctac atcattgatg gcttttactt
     5641 gtccgtttat tttaatgctt atccgtaatt tttctacatt ggaatttcct gaattaacaa
     5701 gtcttaccaa cattttattg acagcattca acgtaaataa aggttgttcc atccagcagg
     5761 aatcgacaaa tatatttttt tcctcatcag atgtaagagg caaaaagtaa gccgtataat
     5821 tcgtgtcaga aagattagcc agcatgtttt tttggaaatc agaaataaag aaacccaatt
     5881 tgtttttcat gcctgccgac tgaaataatg ccgattcttt ttgggcaatt tcatttgtag
     5941 ttttcacggc tgaggtgagt tctaccttat ctacggcatc tatgaaatcc tgcttttgca
     6001 tccagcgctg gtgagagcct tccagatcat tggtcagcaa ggcaaattta tctccagtat
     6061 taaaggcatt ggcaatttcc tttgctttgg attttgccat atccagtccg gattcacctg
     6121 ctttcttcaa ccccatggaa taagagttgt cgatgtaaac ggcgacagca gatgcacctt
     6181 tgtttagttg tgatttttta tcccctaaaa aaggctgtgc aaaagccagc actaaaaaaa
     6241 acagtgcaaa aagacgagag gctaaaatca gtctgttttt gagttcgtca atcgttgttt
     6301 tttcttcatt gacttgtttt aaaaagcgga tgtcagaaaa atagattttc ttatatcgcc
     6361 tgaaatgaaa taaatgaata ataaccggta cagccagtgc agccaacgcc aacagaaacc
     6421 atggaaacac aaaatgcatg tgtcaaatat agataaacta tgcgaatagt atccgcaatt
     6481 tcttgtcggt tatttcctga ataacccaac agatcgtaga tttactatta atttttaaaa
     6541 tgaaatcata tatcagttaa aaatgccgga gctaccaaca actaatgcaa atatgatagc
     6601 aattgcataa atagaaagga caatgatcat cataattcca taaaagcgga atactgaacg
     6661 aattctttta aagctttctt cgagagcaag tgtattattt gtttcaacag ccagttttgc
     6721 tttctgtgaa aactggaaaa ggcgcaaaac aggatatatt gtaattagcc cgataacaat
     6781 aaaataaatg gttaagaaaa tggactgtcc gcttcccatt cctgtatccg ctacagtatt
     6841 gagttttccc ataaatgtgc caataaataa ggcagcaata acaatgagtc cgataaatac
     6901 aaacccaata atggataaga atttggccca tttggctgtt tgtaataaaa aatctttagc
     6961 actagaggtt aaagaaagct cgtttgtaag gtttaaattg ttgtctagta gttcctgatt
     7021 ttccataagt aattttttta taaatatagg gatttgagat gaataagcat tcactttttt
     7081 tataaatttt actccattgg gaattgtatc tttagtgcaa atctattttg aagatgaaac
     7141 ttacatttcc ggataatttt atttggggca catctaccgc agcctaccag attgaaacag
     7201 caggcgaaca taactggaaa ggctttaaga gcaaagacgg cagtatattt gacaaatgct
     7261 ctcagcatga attgcaccgt gaagaagatt taaattatat ctgtcaaatg ggaaatgcct
     7321 accgtatgag ccacgactgg agtaaactgc aaaaggcacc ttttgccgag tttgataaaa
     7381 cagaaacgga agcctacctg aaatttatgc aggaactgaa aaacaggggc atacacatca
     7441 tgttggtgtt gcatcatttc accaatccgc tttggtttga ggaggcaggc agctgggaaa
     7501 aagaaggcaa ccggaaaatg tggctcgact ttgtacaaaa aacggtcaat acattcggtc
     7561 atttagtcga ttcgtggaat acatttaatg aacccaatgt ctatgtcagc aacggctgga
     7621 taaccggatt ttttcctcca ttcaaaaaaa ataaattact gcttgccagg aaagtgctga
     7681 aagaaatgag caaagcacat gaggaggctt atgacatcat caaacaagaa tcaaatgcgc
     7741 caatcggtat ttcatttaac accgtaaagt ttgccggaga agtatttccg gggcatatag
     7801 ctgctaaaat atccgactgg tggtttatgg aatactgcca caatcatttt cttaaagtcg
     7861 attttcaggg attgagctat tatgcccgta ttccgtatcg tccccatcct attgataata
     7921 tcaatacacc cggagtactg gataaactag gcagaaaaca agatttgatg tgggaatatg
     7981 ctcccgaaga attttatcat atttttcatc gctactggaa aaaaacgaaa aagcctatca
     8041 ttattactga aagtggtgtt tgcacttatg atgcagcatt cagaattcaa agtataaaag
     8101 attatttggg ctggatgaaa aaagcgatgg atgacggcat tgatattcgc gggtattttc
     8161 attggagtac tatcgataat catgaatgga atatcggcac gtattataaa tttggcttgg
     8221 tcagtgtgga cttcgaaaca ggaaaacgca ccatgacaaa agccggtgag tttttgggag
     8281 atattgcaaa aaataattcg gtagaagttt aaattatata aacggtcagt aaaaatctga
     8341 gttcgggtta agaaaaagaa ggtgataaaa tagttgattg ctcaaccaaa ttggtttata
     8401 gatttttgca tatcaaagca caagttgtaa atgggattaa ataatgttac ttcaggtact
     8461 cattgctgat atcgcactta tggttgtccg tattcaaaaa atatcatcta ttacatcaga
     8521 tggaattcct tttttcgaat ctggaatata gaagaacctt ctatatgtga agtagtttaa
     8581 tataattaat acagccagtg acaagagtaa gtatttaata aaagtgtcca gacccaaaaa
     8641 cagatagaaa ccaactccga atttattttg attcagataa tacccataac attttacgaa
     8701 ataaaatgca taatctaatg ctgcaattat tatgtagccc aaccaaatgt gatttagctt
     8761 aagtttataa attaaatagc ttaataaaaa cgtaagtaat aatactgcca gatagtccat
     8821 taaatcaaaa atacaaattt ccatagagtt actcgtttta gtatctactt catttggcgt
     8881 atatttggca gatgtctgat ataatccgtt tgctgcctga ccatattgcc aaccaaattg
     8941 ctgccggtga ggtgatacaa cgcccggcct cggtggtaaa agaactgatg gaaaacagta
     9001 ttgatgcagg agcacaacat attcagctca ttattaaaga ttcgggtcgc acactcatac
     9061 aagtggtgga tgatggcaaa ggaatgagtg aaactgatgc ccgcatgagt ttcgagcgac
     9121 atgcaacttc taaaatcaac aaaatagaag atttattttc cttgcataca ttcggtttcc
     9181 ggggcgaggc catggcatcc attgcggctg tagcacatgt ggaaatgaag acaaaaaccg
     9241 ccgacagaga aaatggcgta atcctcatta ttgaaggttc taaggttgta ttgcaagaac
     9301 cgtgctcaca tggaaatgga acttctatct cggtaaagaa tctattttat aatgttccgg
     9361 cacgccggaa ttttctcaaa tccaaccaaa tagaatttaa gcatattctg gatgaattta
     9421 ttcacgtggc attagccaat cctcaaattt tcttttccat gcatcataat ggactcgaaa
     9481 tttatcacct caaatccggc aatttgcgac agcgtattgt gggaatttta ggcaaaaact
     9541 ataatgacaa gctcgttccc atagaagaac ttaccgaata tgtcagcgtg cgtggttttg
     9601 ttggcaaacc tgaactctcc cgaaagacac gaggcgagca atatttcttc gtgaataatc
     9661 gttttataag aaataatttt ctcaatcatg ccgtagcaaa ggcttatgat ggactgatac
     9721 atgataagag ttatccgttt tattgtttgt ttatcgatat taatcctgat gccatagatg
     9781 taaatgtgca tcctaccaaa caagaaataa aatttgaaga cgaaaaatct gtttatctct
     9841 tactgaatgc tgccactaaa catgcacttt cacaatattc cattgcgcct actatagatt
     9901 ttgaacacga gaaaatgttt aatacactgg tagacgaaaa agtgcatttt gaacagcctt
     9961 ttaaagtatt gccatctaaa gccaatacaa atgtttccag caagggttct tatgattcgc
    10021 catttcataa agatatcccc acacatgagc agacctatga gcagtggaaa aaattgtatg
    10081 aaacagatga ggaaacagaa gaaaaggtat tgactattcg cagcaaaatg gatcagttgg
    10141 aagcgttgga aaatgaaaac gaaatcagca acaggccttt taaggatgaa cggtttgagc
    10201 cgatacagct gcacttccgt tatatactca cacaaataaa aagtggattt attcttgttg
    10261 accaatacaa agcacaccaa cgcattttgt ttgaaaagta cttgcgcaat ctgtcacaaa
    10321 cagcaagcgc ttcgcagcag actttatttc ccaaatcttt ggaactgaat ccgtctgata
    10381 ctattatttt acaagagatt ttaccggaac tgaaaatatt gggttttgat attgattttt
    10441 ttggaaaaac aacttttgtt atacatggta tgcccggcga tttggcagat gaaaatgaaa
    10501 ctttattaat tgaaaaattg ttggaagaat ataaaaattc gggtaaaggc gaaactatag
    10561 acaagcgaaa taacgtggca aaaacgcttg cttatcaaac tgccataaaa cccgggaaat
    10621 tattatccaa aatggagatg caaacgttaa tagacgaact gtttgcctgc gagcaaccac
    10681 tgctcaaccc ggcgggattg gcgacatttg tccaatttac tttcgacgaa attgaacata
    10741 aatttaatgc tgtatgagta attttagaag aacgggcggt tttatgcaaa tgacaccatt
    10801 agtcaagaat ttgattataa taaatgtcgc tatcttcctt gttgattacc tgatttttcc
    10861 gcaattaaat agttatttgg ctgtttatta tccatccaat ccggatttta agccctatca
    10921 gttggttacg cacatgtttg cacatgccgg ctttgctcat attttcttta atatgttcgc
    10981 tttgtggaac tttgggtgta ttgtagagca gatgttaggt ataaagcgtt tctttttact
    11041 ctatttcata tccggttttg gagcgatagc tgctcatttg ttaatcaatg gcttgatgat
    11101 ttatcatcat ataggtacgt ttaatttaca accggatgct tttattaata ctgaaggatt
    11161 gaaattactc agtacaaacc cggatgccaa ctggctgccg tatgtgtcca atgttttctt
    11221 acaaaatcaa tatatttcag tgatgctggg tgcttcagga gctattaatg gtatcgtagc
    11281 ggcatttgcg tacttattcc ctaatacgga attgatttta ttgttttttc cggtccctat
    11341 taaagccaaa tacgccattc cgggcatatt gatattatat gatttgggat atggcagttt
    11401 tgcaaatacc ggcgtagcac actatgcaca tatcggcggt gccatcaccg ggtttttgct
    11461 cgtttatttt tggaataaaa ccaacaaaac cacgttttat tagatatatt tatcgcataa
    11521 aataccctca agcaccatga acggaaaatc aatttttgaa gatataaaat ggcagtacat
    11581 atatggcagt aaccttatca ggcttatatt gattaatgtt gccgtgtttg ttgtgctggg
    11641 tttgataaac ctgccttttt tcttttctca atctgatttt aatgtttaca gcttttttta
    11701 cctgtactcc aatccgtcag aattaatgag acatccgtgg ggatttatca cgtacatgtt
    11761 tgtacatcag tctttctttc atttgttgtt taatatgctg gggatgtatt ggtttgggat
    11821 gatagtggaa gatctggtgg gtaaaagcaa gattgttcca ttatacttat atggaggatt
    11881 gtttggtggt atattgtttg tcttgtctta caattttttt gatgcacttt cggtggggta
    11941 ttattcggca attgcattgg gtgcttccgc cagcgtgctg gcatttgttg tggcaggcgc
    12001 agtcattgca cccgattatg aactaaactt gttcatgatt tggcgagtga aactaaaatg
    12061 gatagccttg gcttatgtcg ttttagattt aatcaatatt cccaatggca atgccggagg
    12121 gcatatcgca catattggcg gagccatcat gggatttgtg tatatccgtt tgttgcagaa
    12181 tggaagagat ttggcaaaac cgtattacaa acttgaagat ttctttgttc atctgttccg
    12241 gaaaaaatcg tcggagatga aagtcaataa aaatacatcg tcaaattacg ataaaagcag
    12301 tacaattaag acttctcatc aagataagca agtacgtctg gatgccatac tggacaaaat
    12361 caacaaatcc agttatgaaa gtttaagcaa agaagaaaaa gattttttat ttcaattgag
    12421 caaagaagat tgaaaattat caaatggctg atatcgtttt tatctcgtac tactaatatt
    12481 atagcagtgg cggctttatt gttatgtttg tctgccgcct atctgcatcc tgagaaagta
    12541 tggatgctcc cattgttcgg aatgctcttg ccatttttta tttttgtcaa tctcttgttt
    12601 gtatttatct ggatatggcg cagacgttat tttgccctgg tttctgcatt cgcaattatg
    12661 ttgtgtgttc cacagttact gcacttattt agcttcggta ttttcaaaaa tcatgaagtt
    12721 gcggataaac cgaaaataaa aatcttgtct tataatgtca gagattttga tttgtataac
    12781 tggagcgaaa acagaaattc taaaaatgaa atctttaaga cgataaaaga tattgatgcc
    12841 gacatcattt gctttcagga attttacagc gacacctcac aaactttcaa cacgattaaa
    12901 caactgcaag aagcaggata ttcccattat tattttactc gggagctggt attgcggaat
    12961 acagatgaat ggggtattgc tattttctct aaaaaacaga tagtagacag cggacaaatc
    13021 ttaaagcaaa attttaaaac cggatatggc aaacagccct acaaaggcat ttatgcagat
    13081 tttgcttttt cagatacgac cattcgcatt ttttgtgtac acctgcaatc catttatttt
    13141 ggaaaagatg aataccagac gatagttgag ctgaaagaaa atcagaatat agacgaaaat
    13201 ggggtgatga agattgtggg caaactaaga cgtgcttttg tgcggcgagc caaacaagca
    13261 acggaattga aattgatttt agataaacaa acattgccct atatcgtgtg tggagatttt
    13321 aacgacttgc ctaattctta tgtctacaat accgtttcca gtaatctgaa agacgctttt
    13381 ttagaaaatg gatttggtgt aggttctacc tatagtggca aaattccatt tctgcgtata
    13441 gattatatgt tgagtcagcc aatgctacaa ccggtagcgt tcagaatcga taagaataaa
    13501 atctcagacc atcatcccgt ttgggcagag tatgtgtttg aaaagtaggt tataattttt
    13561 caactttgta tgtgccggat gccggagtta caatattcat gttcatacca aatagtttca
    13621 tcttcatttt aagttcgtaa ttatatggct gttcttgctg aaagtaatcc gctcttttca
    13681 ttgtgatgtt gccgctgcct gtgccatcaa cggttactaa ttttgcatct tctaaattgt
    13741 tgtaaaaaac tgactcacta agaataacaa catcatctct tcttatttct ttcaacgtat
    13801 attttgtttt aatagtgtaa ggcgttaatt catctttaac actaatatct ttagtccaag
    13861 tacttccgat tttaacttct gtgggcggta agatgtcaaa aataccactc atggtcgtgt
    13921 ttatttcatc tgtctgaagt tgatcggaaa gcatcgaatt ttcgtttgac aagaagtctt
    13981 tgctcttttt gattttagcc gcctttaata tcttgtcata gccttccagc gatagtaatt
    14041 tccctcttga atctatattt accttaaact tttttccgat aatattcttt aaactattca
    14101 gttctttctc ttggtcttca tttaaccctt cagatttttt acgagttgaa attgaatacg
    14161 tttcatcttt agcttgtaca gaagtttcga gatattcata gtaagcttcc aatgtatatt
    14221 tttcagaagt agtttctatc acattaaaca ataaacacat ttccattgac atattcatct
    14281 gtatggtttg attggcttct ttatcatctt ccatgctctt tatctgagct tctacagagt
    14341 cttcttcgat gcccatattt agtaaaagtt ttttcattgc ttctgggtca agcttggttt
    14401 ccatctgcat gggtgcattc atcggtatcg tcacacggta agaatcgcct ttcttaagtt
    14461 gcaattgcat gtttatcttc tcttgagcta ttacagtaaa tactgagaat atactcatta
    14521 atacgaatgt gtattttaat aagcgcatat ataatttttt tggaagccaa atataagaaa
    14581 actgtaataa gtggtattgc ctttaaaaaa aataaatgat ttttaaagcc taaacttcat
    14641 ttcgatgcgt gttccgccat ctaaaaatgc cacctcatcg gctaattgac gtattaaaaa
    14701 tacgcctctg ccatcgggtt gctctaaaaa tttgccacag gtagggtcag gtagtttatc
    14761 cggattaaat cctctgccct catccttaat gcttaacttt agcgtggttt cacagcgaat
    14821 ggcagaaata taaacattct tgtcttcatc cgaattattg ccatgctcaa tagcattgtt
    14881 aacagcttca acggaagcaa gcagcatatt gtgatacttg gtgtcgttta attcaaattg
    14941 aatacgcaac gactctacaa gtgtctctac atcaacaata ttttctttac gggatgaaat
    15001 ttttaaatca gcgattttta ccactactta tgttttggtg aaagaacatg caatattata
    15061 tcaaattgcg gtatttgtca ataggcagat tcaagaaagt ttataattcg tcagttcatc
    15121 atgtcgaaat gtgctagaaa ttctgattag attaaaggga atctatggat ttcaagagat
    15181 ctctccctct ggtcgagatg aagggtgctt tactttccgt ttttttcaat cttcacttgc
    15241 atccggatat ccgtaaaggg tcggtctgca ccgtcacgct gagccgctgc aattttatct
    15301 atcacttcaa agccttccac cacttcacca aacacggtat attgcatatc caacatagga
    15361 gtacctccgt tttggccata aatctttatc tgttcttctg tccacggacc atgtcctgtg
    15421 cgctgtccca gcatagtcag ctctgccgga gtataacttt ttccttgtac aatataaaac
    15481 tgcgtagaag aggattcttt tttagggtta ataaaatcgc cggtgcgtgc agcagccaat
    15541 gcaccttttt tatgaatcaa ctttggattg aattctgccg gaacggtgta tggtagattg
    15601 cccgttccca attgttgtcc gggttgggca tgtttagaat taacatcgcc accttgtatc
    15661 ataaactgtg ggatgacacg gtgaaacaac gtcgagtcga gtacgccgtc ttgaacgatt
    15721 tttataaagt tagctttgtg caagggcgtt tcatcataca atcgcaattt cattgtaccc
    15781 aaatctgtgg tgatgataac ataagtagaa gtatccattt tgtttgtttt agtagcagta
    15841 gtttttgttt ttgaggttgt cgaggtttta gtacccgttt tggaagttgt tttttttgta
    15901 acaggtttcg tcgtcgtttt cttttgggcg gttgttgcgg gcttgctctt ggttgtagcg
    15961 ttattctggg ccagcaaatt caatgaaaat aagaatagaa ttaaatgtaa aatttgtttt
    16021 ttcatatctc gatattataa aatttcttcg cggtaaaaat aatcaactat ggcttctttt
    16081 aataaatttt cttgttgatt atgttccagt ttcggagcgt tttttaatgg ctcaaaatga
    16141 ggccagccgt cttcatccag cgccacaaaa gcataataac cttttgccga aagcaacgtg
    16201 catacggcca catggattaa atcttgtttt tgctctttgg aaaattttgt ttgatgttta
    16261 cccaactccc tgtgtccgat taaaaataaa atgccgggca aatccggaac tttgccataa
    16321 accttggaaa ggtgagcttc tatattttcc cagcctgcag gatgttgttc tttattttct
    16381 tgcatattta tcggtctttc cagaaaggat attgattccg gtatttttct aatttttctt
    16441 tacttagtgc aacaatttcg atgccgtttt tttcctgcat atcacataat aagtctcctt
    16501 tataatcaaa cgaacaagaa tgaccattat gtaaatgccc gtggccgtct tcaccaatgc
    16561 ggtttacgcc aattgtatag cactggtttt ctatagctct ggcttgtaat aaagtcatcc
    16621 agtgatgtat gcgcttttgt ggccagctgg ctacaaaaat ctgcacctga acattatggt
    16681 cattttgaca ccacgccggg aaccgcaaat cataacatat aaatggctga atttcccact
    16741 tatcatacga gatgcttatt ttgtttttgc ccgggtaaat taaattggat tcgttttcat
    16801 ttactaacga aaacaaatga tgcttatcgt accggtattg aatcccgttt ttatctaccc
    16861 aaagaaatcg gttatatatt ttatcttctt ccttaattaa aagactgcca caaacaaccg
    16921 tctctttagc acttgccagt tgttgcatcc aagaaactgt gtcgccattt tcagtttctg
    16981 ccaattcggg atcatctaca caaaaagcag tgttaaacaa ttctggcagg atgaaaatat
    17041 cggcagcctg cttcaaggga aaaatcatat cttcaatatg ttccagattt aacttcttgt
    17101 tcttccatgc taaatccggc tgaactagcg cgatgtgcag attatcaaac atgcctgtaa
    17161 aag
//
LOCUS       DAOQII010000018        17093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1588_length_17093_cov_4.763059, whole genome shotgun sequence.
ACCESSION   DAOQII010000018 DAOQII010000000
VERSION     DAOQII010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_17093_cov_4.763059"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLF48_01795"
     CDS             <1..857
                     /locus_tag="PLF48_01795"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TilS substrate C-terminal domain-containing
                     protein"
                     /protein_id="HNF48083.1"
                     /translation="YMQKNEIEYCEDSSNASDSYQRNQIRHHLLPFFTQLNENFKENF
                     ADTVSYMRDYEMLTDEYLQKLKKNGVSTKDGITEIKIGFIRSKQYGKTLLYRILAEFG
                     FNTDQTNLIYQLIQSENEISGKQFYTENHRLVFDRKSILIVDKNIQRDEYLQIDKFPF
                     NLQFNNFKMEGKLIPIEKVNLKKSGKYAFFDASKIILPLLIRYPKTGDYFYPFGMSKP
                     KSSEKAGKKKLSKYFKDEKLSLIEKENTPLLFSGDKLIWVIGQRTDDRFKITDKTFSV
                     LQIKILKS"
     gene            912..1532
                     /locus_tag="PLF48_01800"
     CDS             912..1532
                     /locus_tag="PLF48_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48084.1"
                     /translation="MKKTIKIYMALFAITLFIASCKKDDDSNNNTPTNCTNLTGWIRD
                     GHKWVYSNEPILFFADTLYVSCFSTSTQGVFRSEDLFDDGSFYPIYKSYTKPCGNDLY
                     SSSTEDMANANIPYKLNGSIGNSWTSTTTTTLGYTATNTISIVAKNVSITVPAGTFLC
                     DKYHVISTSNQPGSITVTTDIYLNNDYGMILTDGNTSHYELVRKNF"
     gene            complement(1533..2204)
                     /locus_tag="PLF48_01805"
     CDS             complement(1533..2204)
                     /locus_tag="PLF48_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48085.1"
                     /translation="MGRIFLFPGFGEDAYCFDELLPYLQKSHELFPVDYRPVLDKFTF
                     PLISRMQFCHAIIRYYGIKKQDKIIGHSMGGYLAFQIREIIYNDICLVGSFCDPQKII
                     QFFPQSPRLSQLVAGSGLIKTDFLRNYLLSKIQDERIKTVQEKVMRNMKSFSNTQLAL
                     MLEVSYESKIISEQVNPLRIHAKTDKVVLPPDESYIEVDGGHFCMNLYPEQVASAMKE
                     NDFIS"
     gene            2483..2938
                     /locus_tag="PLF48_01810"
     CDS             2483..2938
                     /locus_tag="PLF48_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48086.1"
                     /translation="MKENYKIKVKVNSEKPDFRVLAAYFFGDDFHDYDSEGNSNPLTS
                     KNWTYLYMCSRQNKELCFEILDINDNPLIFEVSSENIETVNIIAYFLARETNGEIVDE
                     DNNIIPNENLVKKMGNFNLTERLKSADNSIWRTQEEDEKNRVFTCIRVL"
     gene            3065..3709
                     /locus_tag="PLF48_01815"
     CDS             3065..3709
                     /locus_tag="PLF48_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF48087.1"
                     /translation="MDKKHQVHNLIILDESGSMESIKKTIIQGFNEIVQTVKGIEKEF
                     PEQEHFISLVTFNGIEQKLLHFIDPAKKLELIDDTRYKPNASTPLYDAMGFSINKLKQ
                     VLQYQTDYNVLVTILTDGEENASKEYSGNDIKKLIEELKLNRWTFTYIGADHDVEKIA
                     FSLSITNTMIFTKNEEDMKNMFSREKSARAKYSAKIRMNEETSNDFYETEDPKN"
     gene            3730..4317
                     /locus_tag="PLF48_01820"
     CDS             3730..4317
                     /locus_tag="PLF48_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48088.1"
                     /translation="MKKALTLLILFIGINQSFGQDLSYDDFKSLIPYLKAEDWKSAFN
                     ESSKLLKSTQKDTSEFHAIILYINIYSAAGMVTEKKMTYKELEKNVMKYQGQKIIMSA
                     HPVTIKDGALNQIKFEVTDSSNTAFSCATNSKGTNIMCFEKFIFKDKINLDNFSEKSF
                     VRCGGILEKIEMNPNKSLIWVLRLTVTDAFARNAN"
     gene            4581..5099
                     /locus_tag="PLF48_01825"
     CDS             4581..5099
                     /locus_tag="PLF48_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48089.1"
                     /translation="MEHSLKIKLIKKKRRWNDVGPFAFKFIPVILISIIISLLLINGY
                     NYVYTYLILILIYILYLTIFDNRKYDWWLVNYDITGILFLKNDSIEIEENGIRSIYKV
                     NKLHKIQIKENFFQNYTRMRDIVHDGIGSILIDNGINKNLAFFLIENESDLESLHALK
                     AEWKRLGLSIWT"
     gene            5303..5908
                     /locus_tag="PLF48_01830"
     CDS             5303..5908
                     /locus_tag="PLF48_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48090.1"
                     /translation="MGAEIHIRLTSDRHNNKNLAPFLGRDFQYEISEGSIWQNKRSFL
                     NSDEIKLLKATYDELFENKDLIPTIEPKSLKEVLVKVKNYLRLNQSELPFEIEIDYEK
                     MEKENLNYYLTVNESRCWIKDDSKYNNVFNKVQIVNYKMELNKVDIWIDISDKVEIEG
                     KIYYLKKTTRYEKYADLIDKVIDFCRHAEDIKNKIYWIYTH"
     gene            6055..7080
                     /locus_tag="PLF48_01835"
     CDS             6055..7080
                     /locus_tag="PLF48_01835"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNF48091.1"
                     /translation="MIELSQIDRIKIKLRLAKNTDSFFEVFGSSSHKYFLDKPLELIE
                     VEQFECEYNIMLPEGYKKFISEIGNGGINYENSVVGNSGAGPNYGIFKLGHPYQFISD
                     PSLKYLEQQPFLTEETTDEIWEYIYLKMEDNISDEDYEIEIAKAYAGILNIGYAGCSE
                     YLGIIVNGKNKGKIIDTYDEIEYCPCFYKENDFLDWYENWLDTIISGKKIKESGFTNE
                     TEESCINRFLSDKESYWKFVSLSSLRNFDKLNKSSIKTLKNKYNTEKDEKVRLYILNL
                     LTKFDYDNTKKEIATLHRKEPLEFLRNIHLFAQEKSVEWKNEIDIIRNVNFNNQEIIE
                     YIKYIFE"
     gene            complement(7213..8382)
                     /locus_tag="PLF48_01840"
     CDS             complement(7213..8382)
                     /locus_tag="PLF48_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48092.1"
                     /translation="MLFKDVIGQTAIKERLMQMVQQDKIPHAQLFLGDSGVGTLPLAL
                     AFTQYVVCENKTGTDSCGQCAPCIKAGKLIHPDIHFTFPTVSGVKKSKKGKGNDDDDE
                     KKTSKPVSADFIEEWRKLILAKPYASELDWIRSITEENKQANITADEARTIIRNLNMT
                     PFEAKYKIHLIWMAENLGKEGNILLKLLEEPPQNTILILVAENQEELLSTIISRCQIL
                     KIPKIQDQFIKAELIQIGVTQSKADHIAYLSDGNYYAALSLAESETDKQIDLLNTWFE
                     YAIYMKVPELFRWVEETSKMGRENIKSFLEYVTHLLHESMLFKLIPDYNPRLTEDEID
                     LCNRLNRSIIPDKLEALLALINKKHYEIERNVNPKMVLMDMSLKINQLVHGKAIE"
     gene            8428..8835
                     /locus_tag="PLF48_01845"
     CDS             8428..8835
                     /locus_tag="PLF48_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNF48093.1"
                     /translation="MKTAVIRRSHFNAAHQLWNKQWDKAKNKEIFGLCANENYHGHNY
                     IVEIKVTGEVDPDTGYVLDLKILNQLIHEEVYKPYDHKNLNLDTEDFKDLNPTVENIA
                     RVIYEKIRKRLATDLELQVKIWETDNNMAIYPA"
     gene            complement(8844..10409)
                     /locus_tag="PLF48_01850"
     CDS             complement(8844..10409)
                     /locus_tag="PLF48_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNF48094.1"
                     /translation="MQEHYDYVIIGSGFGGSVSALRLSEKGYKVLVIEKGKWFNQPED
                     FPKTNWNLRKWMWMPSMKMHGLFNLSFFRHVAVLSGVAVGGGSVVYANTLPTPKSEFF
                     NSGHWHGLENWEDTLKPFYDTANKMLGAVKHPYMSRSDEVMLELAKQTNNEAGFDRTN
                     VAVYFGEPGVTVNDPFFEGKGPTRTGCNECGGCMLGCRYNAKNTLDKNYLHLAQQLGC
                     EIIAEKEVYDVVPLSADGSAGYEIKFRDSLKYFKSKNSVKAKGVIFAGGVLGTVELLL
                     KLKGTSLPNLSDKIGQGVRTNSESLIGVTSFDDISFSKGIAIGSIINIDKSRHIEPVK
                     YSEGSGFWRIFMAPMVQGSNILVRFLNMMKDYITQPVRNLKAFFIDDWSKRTNILLYM
                     ESIDSSLSFARNIFGKLKTHLDSGPAPTAFNPKAQELAHKVEKIINGKAMVMSTETLL
                     GIPTTAHILGGACMGKDVHSGVIDKDNHVFNYQHMLVCDGSMISANPGVNPSLSITAI
                     SERAMSKIPKKEE"
     gene            11027..12433
                     /locus_tag="PLF48_01855"
     CDS             11027..12433
                     /locus_tag="PLF48_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNF48095.1"
                     /translation="MKTFNIDIQYKKIIADILTPISIYLRLRNKYSDCILLESSDYHG
                     EEHNYSYICFNAISGFTVESNSFIKQFPQQDKEVTDLSKTNLEDEILRFISSFEITSK
                     PTLSCMYSGLFGYMTYDIVRYFEQIELRHFSTEIPVAQYKLYQFIVAYNHFNHEVILI
                     KNEIADSNTAGTSLDEIETIIKYKPMLLNPFSAEQTKSSNFTDEEFLAIVEKGKQSCY
                     RGDVFQLVLSRQFTKSYRGDDFNVYRALRSINPSPYCFYFDYGTFRLMGSSPEAQLII
                     QDKKVTIHPIAGTFKRSGDDLKDAALAQQLSNDPKENAEHIMLVDLARNDLSKCGEET
                     RVEVYKEVQYYSHVIHLVSKVSAVLDSIPAKIKTIAATFPAGTLSGAPKYKAMQLIDE
                     YEKTPRGFYGGCIGHLDFTGDFNHAIMIRTIQSKAHTLTFQAGAGVVADSIAMNELQE
                     VENKLAAVNKAIELAAQL"
     gene            complement(12442..13308)
                     /locus_tag="PLF48_01860"
     CDS             complement(12442..13308)
                     /locus_tag="PLF48_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48096.1"
                     /translation="MHEVKHEYHEVNGIKLHIGRKGNGKKLVVLLHGWPEFWYTWRYQ
                     IPVLANYYTVVAPDLRGFNLSDKPIGIEQYKTDIVASDIAELIKKLGFEKAHIVGHDW
                     GGATAWTFAALYPELTDKLVVLNCPHPKVMLKSLKSNPVQIFRSWYMFYHQIPLLPEY
                     LYDNVLPLFFKQFFRGWMYNKQNFSDADLKAFVLAFRQTGALTGGLNYYRAMMQTKPN
                     TSIFRNKITADTLLIWGEGDRALGKELTNNTADYIAGDFQLNFIQNCSHWTQNDCPDE
                     VNNHLLNFLGAE"
     gene            13857..16511
                     /locus_tag="PLF48_01865"
     CDS             13857..16511
                     /locus_tag="PLF48_01865"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNF48097.1"
                     /translation="MELAKNISPAEIEEKWYAHWLAKNYFHSEPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARMQEKNACWVPGTDHASIATEAKVVAMLKEQGIDK
                     KQLTRDEFLTYAWEWKEKYGGIILKQLEKLGASCDWQRTKFTMDASLSDAVTDVFIDL
                     YNKGYIYRGVRMVNWDPKGLTAVSDEEVIHKEVNSKLFYIQYAICSDDSQLPTANFVT
                     IATTRPETILGDTAICVNPNDERYQHLKGKFAFVPLINRKIPIIFDEYVDMEFGTGAL
                     KVTPAHDINDYNLGIKHHLETIDILNDNGTLNEKAQIYVGEDRFAVRKKIVAELEEKG
                     CLAKVEDIKNKVGYSERTDAVIEPKLSMQWFLKMEELSKPALKAVMEDSVKLIPEKFK
                     NTYRHWMENVRDWCISRQLWWGHRIPAWYDDKGKIAVAKTEEEARIKLGIQDITTTIR
                     QDEDVLDTWFSSWLWPISVFDGFQDKDNKDINYYYPTNDLITAPEILFFWVARMIIAG
                     YEYRGKKPFNNVYLTGIVRDKQGRKMSKSLGNSPDPLDLIALYGADGVRTGMLFSSPA
                     GNDLLFDEKLCEQGRNFSNKIWNALRLVKGWEINSDNKNQESRDKTNALETQHSKFSS
                     WFENKLQQVIKTTDELYAQYRLSEVLTTLYSFIWDDFCSWYLEYVKPPFGEKIDKATY
                     EQSVHYFETLMKLMHPFMPFITEEIYHHLNENRTDDIIVAKYPLAAEIHQQAIADGEK
                     AKQFISAVRDIRNRNGISPKQTLEVWVQTEDTSFYNAFENALVKMANISSVQFTDKEI
                     ENAVTVLLNKDKLYIITGIEVNKDDEKQKLEKEMEYYKGFIASIEKKLSNEKFVQNAK
                     PEVVENERKKIADGQAKLKALEDSLAKL"
     gene            16523..>17093
                     /locus_tag="PLF48_01870"
     CDS             16523..>17093
                     /locus_tag="PLF48_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNF48098.1"
                     /translation="MADKKRYTITAALPYANGPLHIGHLAGAYIAADIYARYLRLKGE
                     DVIFVCGSDEHGAAITIKAKKENKKPKEIIDEYHTVIRDSFEKFGIAFDIYHRTSEPV
                     HYDTSQEFFRILNDKNEFDIIESEQYYDKEAKQFLADRYITGTCPKCGNESAYGDQCE
                     KCGSSLNPTDLIHPKSTLTNTSPVLKKTKH"
BASE COUNT         6010 a         3006 c         2923 g         5154 t
ORIGIN      
        1 cttatatgca aaaaaatgaa attgaatatt gcgaagattc aagtaatgcg agtgacagct
       61 atcagcgcaa ccaaataaga catcatctcc tgcccttttt tactcagctg aatgaaaact
      121 ttaaagaaaa ttttgctgac accgtctctt atatgcgcga ttacgaaatg ctgacagatg
      181 aatatttgca aaagttgaaa aaaaatggtg tcagcacaaa agacggaatc acggaaatta
      241 aaataggatt tatccgttcg aaacaatacg gcaaaacttt actgtatcgc atcttagcag
      301 agttcggttt taacacagat caaacgaact taatttatca gttgatacag tctgaaaatg
      361 aaatttcagg taagcaattt tatacagaaa atcatcgtct agtatttgac agaaaaagca
      421 tattgatagt ggacaaaaat attcagcggg atgaatattt acaaatcgac aagtttccgt
      481 ttaatctgca atttaataat tttaaaatgg aaggcaagct gataccgata gaaaaagtaa
      541 atcttaaaaa atccggaaaa tatgcctttt ttgatgcatc aaaaatcatt ttacctttgc
      601 tgattcgtta cccaaagaca ggtgattatt tttatccttt cggcatgagc aagccgaaat
      661 cttcggagaa agccggcaag aagaaattgt ctaaatactt taaagatgaa aaattaagct
      721 taattgaaaa agagaatact cccttgttgt tctccggtga caaattgatt tgggtaatcg
      781 ggcaacgaac agatgatcgt tttaagatta cggataaaac tttcagtgtt cttcaaataa
      841 aaatattaaa aagttgaact ttttctttgt ttaatgtaat attctgttat ttttgtaaga
      901 aattattttt tatgaaaaag acaataaaaa tttacatggc tttatttgct ataacacttt
      961 ttatagcttc ctgtaagaaa gatgatgatt caaataataa tacccctaca aattgcacca
     1021 acctgactgg atggatcaga gacggacaca aatgggttta ttcgaatgag ccaatattat
     1081 tctttgcaga caccctatat gtcagctgtt ttagcacatc tacccaaggt gtatttagat
     1141 ctgaagattt gtttgatgac ggcagttttt atccgattta taaatcatat acaaaacctt
     1201 gtggaaatga tttatatagt tcatctaccg aagacatggc aaatgcaaat attccataca
     1261 aattaaatgg gagtataggc aattcatgga catcaactac tacaactacg cttggctaca
     1321 cggcaacaaa tacaataagt atcgttgcaa aaaatgtatc tataacagtt cctgctggta
     1381 catttctttg tgacaaatat catgtaattt ccacttccaa tcagcctgga agcattacag
     1441 tcactacaga tatctattta aacaatgatt acggaatgat attgacagat ggtaatacat
     1501 cgcattatga attagttcgt aaaaactttt aattagctga taaaatcatt ttctttcatg
     1561 gcagatgcca cttgttcagg atagagattc atacaaaaat gtccaccgtc aacttcaata
     1621 taagattcgt cgggtggcaa tactacttta tcagtctttg catgaatacg caatggattt
     1681 acttgttctg aaattatttt actctcgtaa gaaacttcca acatgagagc caactgcgta
     1741 ttgctgaatg atttcatatt gcgcatcact ttttcctgaa cggtttttat acgttcatct
     1801 tgaattttgc tcaaaagata atttctcagg aaatctgttt ttattaaacc ggagccggct
     1861 actaattgtg acagacgggg tgattgcgga aaaaactgaa taattttctg tgggtcacaa
     1921 aatgaaccta ccagacaaat atcgttataa ataatttcgc ggatctgaaa agcaaggtat
     1981 ccgcccattg aatgaccgat tattttatct tgttttttta tgccgtaata acgtattatg
     2041 gcatggcaaa actgcattct gctgattagt ggaaaggtaa atttatctaa caccggacga
     2101 tagtcaaccg gaaaaagttc atggcttttt tgtaaataag gcaacaattc atcaaagcaa
     2161 taagcatctt cgccaaaacc cggaaataaa aaaatacgcc ccatattatg actcaatttg
     2221 cacaaatata agcaaactaa aaaaatctga aattaagtaa gcttgagata cttacaaaat
     2281 aatacatttt tttgcctagt ttaaaaagaa gtaaaaaagc aaacttgcaa ctacattact
     2341 ttactctata aaatatacat ctcctattgc catttttgaa acaaataact acctttaaaa
     2401 aacaaaaaag gcggaacgga ggttttctct taggcttagt tcattaacca aattgacgac
     2461 atataattat ttaatattat aaatgaaaga aaattacaaa attaaagtaa aagtaaattc
     2521 tgaaaaacct gattttaggg ttttagcagc atattttttt ggtgacgatt ttcatgatta
     2581 tgatagtgag ggaaacagta atcctttaac aagtaaaaat tggacttatt tatatatgtg
     2641 ctctaggcaa aacaaagaat tgtgttttga aattcttgat ataaatgaca acccattgat
     2701 ctttgaagta tcgtcagaaa atattgaaac tgtaaatatt attgcatatt ttcttgcaag
     2761 agaaactaat ggagaaatag tagatgaaga taataatatt atacctaatg aaaatttagt
     2821 aaaaaaaatg ggaaatttta atttgaccga acgattaaaa tcggcagata atagtatttg
     2881 gagaacacaa gaagaagacg aaaaaaatag agtatttaca tgtataagag tattgtaaat
     2941 gtatcataaa aaagaattcg tccctttata aaattagaaa aacaaaaaaa cggatgtact
     3001 taaacagtct ctcatttaca gccaattttt aacccgataa cagaaaaact ttaaatttta
     3061 tattatggac aaaaaacatc aagtacacaa cctcattatt ttggacgaga gcggctcaat
     3121 ggagtcgatt aagaaaacaa ttatccaagg ctttaacgaa attgtacaga ctgtaaaagg
     3181 aattgaaaag gaatttcctg aacaagaaca ttttatctct ttagtaacat ttaacggtat
     3241 tgaacaaaaa ttactgcact ttattgaccc tgcaaaaaaa cttgagttaa ttgatgatac
     3301 aagatataaa ccaaacgcat ctacaccact ctatgatgca atgggattca gtattaacaa
     3361 gcttaaacaa gtattacaat atcaaacaga ctataatgtg cttgtgacaa ttttgacaga
     3421 cggcgaggaa aatgcttcta aggaatattc aggtaatgac attaagaaac tcattgaaga
     3481 actaaaactt aaccgttgga catttacata cattggagct gaccatgatg ttgaaaaaat
     3541 tgctttttca ctctctataa ctaacacaat gatttttact aaaaacgaag aggatatgaa
     3601 aaatatgttt tcaagagaaa aatctgcgcg agctaagtac agtgcaaaaa tacggatgaa
     3661 tgaagaaacg agtaatgact tttacgaaac agaagatccg aaaaattaat cctgataatt
     3721 tatacaataa tgaaaaaagc attaacactt ctaattttgt tcatcggaat aaatcagtca
     3781 ttcggacaag acttatcgta tgacgatttc aaaagtttaa ttccctattt aaaagctgaa
     3841 gattggaaat ctgctttcaa tgaatcctca aaacttttaa aatctacaca aaaggacact
     3901 tctgaatttc atgccataat tctatacatt aatatctatt ctgctgcggg tatggtgact
     3961 gaaaaaaaaa tgacttataa agaacttgag aaaaatgtta tgaaatatca aggtcaaaag
     4021 attatcatgt cagctcatcc tgtgacaatt aaagatggtg ctttaaatca aataaaattt
     4081 gaggtaacag attcaagcaa caccgctttt tcttgcgcaa caaattcaaa aggaacaaat
     4141 attatgtgct ttgaaaaatt tatatttaaa gacaaaataa accttgataa tttttctgaa
     4201 aaatcgtttg ttcgttgtgg tggaatacta gaaaaaatag agatgaatcc aaacaaatca
     4261 ttgatttggg tattgcgact taccgttacg gatgcatttg caagaaatgc aaactaatag
     4321 catgaaacaa caaaagcact aaaaccaaat aaaatgctta tcacttgaac aacttcacat
     4381 ttataattcc cgttctttta aaattacaaa tccatcaata ttgtaaatga tttacatagc
     4441 aatgaaagaa ttactactct ttactttact ctaaaaaata gacatctacc agtgtcagat
     4501 atggaactta taactatcat taaaaaatcg aaaaatagca taatgaggat ttcttagagg
     4561 catactttaa ttagactatt ttggaacact ctctaaaaat aaaacttata aagaagaaac
     4621 gtcgctggaa cgatgttggt ccgtttgctt ttaaatttat cccagttatt ctaatctcaa
     4681 taattatatc cttactctta ataaatggct ataattacgt ttatacatat cttatattaa
     4741 ttttaattta cattttatat ttaacaatat ttgataatag aaaatatgat tggtggttag
     4801 tgaattacga tattacaggc attctgtttt taaaaaatga ttctattgaa attgaagaaa
     4861 acggaataag gtcaatctat aaagtaaata aacttcacaa aattcaaata aaagaaaact
     4921 tttttcaaaa ctacacaaga atgcgtgata tagttcatga cggaattgga tcaattctaa
     4981 ttgataatgg aatcaataaa aacttggcat tcttcttaat agagaatgaa tcggatttag
     5041 agtctctaca tgcactgaaa gcagaatgga aaagattggg tttatcaatt tggacataaa
     5101 ctaacgacta agcttcattt tacaaaacgt agaaaaagct aatgtaagac tagtatgatt
     5161 taatgacaaa acaatattga agaaaaaata tacatcccct attgccattt ataaagcaaa
     5221 tacctacctt taaattacgg aatttgcgga aaatgaaggt tttttcacag actgccgtta
     5281 gcaaacattt aaagaattca aattgggtgc agaaattcat attagactta cgagtgacag
     5341 gcataacaat aagaatcttg cgccattttt aggcagagac tttcaatacg aaatatcaga
     5401 aggcagtata tggcaaaaca aaagatcatt tctaaattca gatgaaataa aacttctaaa
     5461 agcgacttat gatgagttat ttgagaataa agatttaatt ccaactattg aaccaaaatc
     5521 tctaaaagaa gtacttgtca aagtcaagaa ttacttaagg ttaaatcaat cagaattacc
     5581 atttgaaatt gagattgatt atgaaaaaat ggaaaaagaa aatttaaatt attatttaac
     5641 agttaatgaa agtcggtgct ggattaaaga tgattcaaaa tacaataatg tgttcaacaa
     5701 agttcagatt gttaattata aaatggaact caataaagtt gacatttgga ttgacatatc
     5761 tgataaggta gaaatagaag gtaaaattta ttatctaaag aaaacgacta ggtatgaaaa
     5821 gtatgcagat ttaattgaca aagttattga tttttgtaga cacgctgaag atattaagaa
     5881 taaaatttat tggatataca cacactaata aacgtttgct aacaaaaaat tggcactagt
     5941 tgtggtagat ttggtaattc aagttttggg tgttatattt aatttgtaaa taagtgttgg
     6001 ttcgtgctat acacgcacaa ccagcgccaa ttttcagccg ttagcataaa ttttatgatc
     6061 gaactatctc aaattgacag aatcaaaata aaattaagat tagcaaaaaa tactgattca
     6121 ttctttgaag tatttggttc ttcttctcac aaatattttt tagacaaacc attagaatta
     6181 attgaggtag aacaatttga atgtgaatac aacattatgc ttcctgaagg ttataaaaaa
     6241 tttatttccg aaattggaaa tggaggaata aattatgaaa attctgttgt tggaaattct
     6301 ggtgcaggac ccaactatgg tatattcaaa cttggtcacc cttatcaatt catttcagac
     6361 ccttccctaa aatatcttga acaacaacct tttctaactg aagaaacaac ggatgaaatt
     6421 tgggaatata tatatcttaa aatggaagac aatatttcag atgaagatta tgaaatagag
     6481 atagcaaaag cctatgctgg aatattaaat attggctatg ctggatgttc tgaatattta
     6541 ggaataattg ttaatggaaa aaataaagga aaaataatag acacctatga tgaaattgaa
     6601 tattgtccgt gtttttataa agaaaatgat tttttagact ggtatgaaaa ttggcttgat
     6661 acaattattt caggcaaaaa aataaaagag agtggattta ctaatgaaac tgaagaaagc
     6721 tgcataaacc gatttctatc agacaaagaa agctactgga aattcgtttc cctctcatct
     6781 ttaagaaatt ttgataaact gaataaatca tcaataaaaa cactaaaaaa taaatataat
     6841 acagaaaaag atgagaaagt aaggctttat attcttaatc ttttgacaaa atttgactat
     6901 gacaatacca aaaaagaaat agcgacatta cacagaaaag aaccattaga atttttacga
     6961 aatatacatt tatttgccca agaaaaatca gttgaatgga aaaatgaaat cgatattatt
     7021 agaaatgtaa attttaataa tcaagaaatt atcgaataca ttaaatatat ttttgagtaa
     7081 aaaacgtcat ctaacatcgg tagcctttgc acaactacat ttttttagga ctatattatc
     7141 aatatttatt aataatacaa ctatttttca atacctgttt tgctaattgc tgtaagtaaa
     7201 tacaccatta ccctattcta ttgccttgcc gtgtaccagc tggtttattt tgagcgacat
     7261 gtccattaag accatcttgg ggtttacgtt gcgctctatt tcataatgct tcttattgat
     7321 taatgcaagc aaggcttcca gcttgtcggg tatgatgctt ctgtttagcc ggttgcacaa
     7381 atctatttca tcttccgtca gcctcgggtt gtaatcgggt atcaatttga aaagcataga
     7441 ctcatgcagc aaatgcgtta catattccaa aaaggatttg atattctctc tccccatttt
     7501 tgaggtttcc tccacccacc tgaacaactc gggcaccttc atgtaaatgg catattcaaa
     7561 ccaggtattc agcaagtcta tctgtttatc ggtttcactt tctgccagcg ataaggccgc
     7621 gtaataattt ccatccgaca aatatgcaat atgatcggct ttgctttgag taacgccaat
     7681 ttgtatcaac tcggctttga taaactgatc ctgaattttt ggaatcttta aaatctgaca
     7741 gcgcgaaata atggttgaca acagctcttc ctgattttct gctactaaaa tcaagatggt
     7801 attttgtggt ggctcttcca gcaacttcaa caaaatattg ccttctttgc ccagattttc
     7861 tgccatccaa atcaagtgaa ttttatattt cgcctcaaat ggcgtcatat tcagattccg
     7921 aatgattgtg cgggcttcat cagccgtaat attcgcttgt ttattttctt cggtaatgga
     7981 gcgtatccag tcgagctccg aagcatatgg ttttgccaaa atcaacttgc gccattcttc
     8041 tataaaatct gccgatacgg gtttactggt ctttttttcg tcatcatcat cattgccctt
     8101 tccttttttc gactttttaa caccgcttac cgtcggaaaa gtaaaatgaa tatccggatg
     8161 aatgagtttg ccggctttta tacaaggtgc gcactgtccg caactgtccg tgccggtttt
     8221 attttcgcaa accacatatt gtgtaaaggc aagagccagc ggcaaagtac ccacacccga
     8281 atcgcccaaa aacaattgag catgcggtat tttatcctgt tgcaccattt gcatcaggcg
     8341 ttcttttatg gcggtttgac ctatgacatc tttaaacaac ataaagctct gctatttttt
     8401 gtatttttac agcgaaaaca aagatacgtg aaaactgcag tcataagaag atcacatttc
     8461 aacgcagcgc atcaattgtg gaataagcaa tgggataagg caaaaaataa agaaatattc
     8521 gggttgtgtg ccaatgaaaa ttatcatggc cataattata ttgtagaaat aaaagtaacc
     8581 ggagaagtgg atcccgatac cggatatgtt ttggacttga aaatccttaa tcaactgata
     8641 cacgaggaag tgtataaacc ttacgaccat aaaaacctaa atctggacac cgaagatttt
     8701 aaagacttga acccgacggt agaaaatatt gccagagtga tttatgaaaa aatcagaaag
     8761 cgattagcaa ctgatttgga attgcaggta aaaatttggg aaacggataa taatatggca
     8821 atttatccgg cttaacagat aaactactct tcttttttcg gtattttact catggctctt
     8881 tcgctgatag ccgtaatact taaactggga ttcacgccgg gattggcaga tatcatgctg
     8941 ccatcacaaa ccagcatatg ttgataattg aaaacatgat tatctttatc gataacgcca
     9001 ctatgcacat ctttgcccat gcatgcaccg ccgagtatat gtgcagtggt aggtatgcct
     9061 aataaagttt ccgtactcat caccatggct tttccgttga taatcttctc tactttatgt
     9121 gccagttcct gtgctttggg gttgaaggct gtgggtgcag gacccgagtc taagtgagtt
     9181 ttcagtttgc cgaaaatatt acgggcaaag ctcaatgaag aatctatact ttccatatac
     9241 agcaaaatat tggtacgctt gctccagtcg tcaataaaaa aggctttaag attgcgtacc
     9301 ggctgcgtga tatagtcttt catcatgttt agaaaacgta ccagaatatt acttccctgc
     9361 accataggag ccataaaaat gcgccaaaaa ccgctacctt ccgaatattt taccggctca
     9421 atatgacggc ttttatcaat attaatgata gagccaatgg caatgccttt tgaaaatgaa
     9481 atatcatcaa aagacgttac gccaattaaa ctttcagaat tggtgcgcac accctgccct
     9541 attttatcag ataaattcgg caatgacgtt ccctttagtt tcagcaatag ttcgactgta
     9601 cccagcacac ctccggcaaa aataacgccc ttggctttta cactgttttt gcttttaaaa
     9661 tattttaatg aatctctgaa tttaatttca tatcctgcac ttccatctgc actgagtggc
     9721 actacatcat aaacttcttt ttctgctata atttcacaac ccagttgttg tgctaaatgc
     9781 agatagtttt tatctaatgt atttttggca ttatagcgac agcccagcat acagcctccg
     9841 cattcattac agcctgttcg tgtaggacct ttgccctcaa aaaacggatc atttactgtt
     9901 acaccgggct caccaaaata aacggctaca ttggttctgt caaaaccagc ttcattattt
     9961 gtttgcttag ccagttccag catcacttca tcactcctgc tcatatatgg atgcttaaca
    10021 gcacccagca ttttatttgc tgtatcgtaa aaaggtttta acgtatcttc ccaattttcc
    10081 agtccatgcc aatgccccga attaaaaaac tcagatttag gtgtcggcaa ggtattggca
    10141 tacaccactg agcctccgcc cacagcaaca ccacttaaga cggctacatg cctgaaaaaa
    10201 ctcagattaa acaagccgtg cattttcata gacggcatcc acatccattt gcgcaagttc
    10261 cagtttgttt tgggaaaatc ttcgggttgg ttaaaccatt ttcctttttc gatcaccagt
    10321 actttgtatc ctttttcaga taaacgcaat gcggaaactg aaccgccaaa accgctgcca
    10381 ataatgacat aatcgtaatg ttcttgcata acttaattac agcataaaat taagctacaa
    10441 tataactaaa agtcggaata cgtaaaatag acaacagata aaataagcgg acaatgaaat
    10501 attgccagtc agatattttg agttgtatca gacgatatgc tttcaaatgc gttgattttt
    10561 taataaacca ccccctgccc ctcccttatt taggagtttt tttagaaaaa cctgcaaata
    10621 aaaaattgaa atacaaaacc cgaactctga ttttttaact aatttgagtt taaacaaacc
    10681 gcccttaatc catacttgaa ttaagaaaaa acaaaagcaa tcatcaaatt tatattgaat
    10741 tgcttaaccc taacttaggt tttataggct tgggcaaaaa taaaccaccc ccagccccct
    10801 cctaaattta ggaggggggt gataaaaaca gctggtgcat tattttttta gtgatatgga
    10861 atgataaaca aaaattaaaa tataagcgtg ctgaaatttt tatacttttg aattatgcaa
    10921 aaacaacatg gaaattttat ttggtggcct tagtcaatac tatttggctt aactttatat
    10981 tttataacag tcagccttta tgctggcttt tttaattttt ataaaaatga aaacattcaa
    11041 catcgacatt caatacaaaa aaatcattgc ggatatcctg actccaatca gtatttattt
    11101 gcgtttgcgc aacaaatatt cggattgtat tttactcgaa agctccgatt atcatggtga
    11161 agaacacaac tattcgtata tctgcttcaa tgccatttcc ggatttacag ttgaaagtaa
    11221 ttcttttatc aaacagtttc cacaacaaga taaagaagtg acggatttaa gcaaaacaaa
    11281 tcttgaagat gaaatactcc gctttatttc ttcctttgaa attacttcaa aacctacgct
    11341 cagctgtatg tacagcggac ttttcggtta tatgacctat gatatagtgc gctattttga
    11401 acaaatcgaa ttgcgtcact tttccacaga aattccggta gctcaatata aattatacca
    11461 atttatagtt gcatataacc attttaatca tgaagtcatt cttataaaaa atgaaatagc
    11521 agacagcaac acagcaggaa cttcactgga tgaaattgag acaataatca aatacaaacc
    11581 gatgctgctg aatccgttta gtgctgaaca aacaaaatca tcaaatttta cagatgaaga
    11641 atttttggca atcgtagaaa aaggcaaaca aagctgttat cgcggagatg tatttcagtt
    11701 agtattatcg cgacaattta ctaaatcgta cagaggcgat gatttcaatg tttacagggc
    11761 tttgcgcagt attaaccctt caccttattg cttttatttc gattatggaa ctttcagatt
    11821 aatgggatcc tctcccgagg cacagttaat aatacaagat aaaaaagtaa ctatacatcc
    11881 cattgcaggc acatttaaac gcagcggaga tgatttaaaa gatgctgcat tagcccaaca
    11941 actcagcaac gatccaaaag aaaatgcgga acatatcatg ctggtggatt tggcaagaaa
    12001 tgatttatcc aaatgcggtg aagaaacgag agttgaagtg tataaagaag tgcagtacta
    12061 ttctcatgtc atacatttag tgtcaaaagt gagtgcggtt ttagattcta ttccagccaa
    12121 gataaaaacg atagcagcta catttcctgc aggaacacta agcggagcac caaaatacaa
    12181 ggcgatgcag ctgatagatg aatatgaaaa aacaccgcgt ggattttatg gcggatgcat
    12241 aggtcatctg gattttaccg gagattttaa tcatgccatc atgatacgca cgatacaaag
    12301 taaagcgcac acattaactt tccaagccgg tgccggagta gtagcagatt ctatagctat
    12361 gaatgagcta caagaagtag aaaataagtt agctgctgtt aataaggcaa tcgaacttgc
    12421 tgctcaactt taaggaataa cttattctgc ccccaaaaaa ttaagtaagt ggttattgac
    12481 ttcatcagga caatcatttt gtgtccagtg ggaacaattc tgaataaaat ttaattggaa
    12541 atctcctgca atataatctg ctgtattgtt tgtaagttct ttgcccaatg cacggtcgcc
    12601 ttcgccccaa atgagtaaag tatcagctgt aattttattt ctgaagattg aagtatttgg
    12661 ttttgtttgc atcatggctc tgtaataatt gagtcctcca gtaagtgctc ccgtttgccg
    12721 aaatgccagt acaaatgctt ttaaatcggc atcagaaaaa ttctgtttat tatacatcca
    12781 gcctctgaaa aattgcttga aaaataaggg caaaacatta tcgtataagt actccggcaa
    12841 caaaggaatt tggtgataaa acatgtacca gcttctgaaa atctgtaccg gatttgattt
    12901 gagagatttg agcatcacct tgggatgcgg acaattgagt accactaatt tatcggtgag
    12961 ttcgggatac agggctgcaa aagtccaggc tgttgcacct ccccaatcgt gccctactat
    13021 atgcgctttt tcaaaaccta atttttttat cagttcagca atatcactag ccacaatatc
    13081 tgttttatat tgttctatac cgattggttt atcacttaaa ttaaacccac gcaaatcggg
    13141 tgcaactacc gtgtaatagt tagctaaaac cggaatttga taacgccagg tgtaccaaaa
    13201 ttcaggccag ccatgcagta gtacaacaag ttttttacca tttccttttc tcccaatatg
    13261 cagttttatc ccatttactt cgtgatattc atgtttaacc tcgtgcataa tatatttttt
    13321 ctcaagttac agaattaaag caccaaatta aaatttggaa atacattttc aacaggaagt
    13381 tcaataaatt aacccatgtt aacgcttttt agaagtatga aaatttacta acctgagttc
    13441 gggatgaatg ttttaacttg aaaaatagtt gatttttttt tagaaaccac ctcctttaac
    13501 tttctcctcc cttgttaaat tggacgaaaa gataagaaat caactatttt taatcatcgg
    13561 taggtgtcct cacctaacaa aagtagcttt taaattgttc tatagtttaa accacataga
    13621 tgcatagcat tttttttaac ttaaataatc gattggagca aatccgttta gatagactgt
    13681 ctcgtcctga aataggttta cacatttata actcaacccc tctcctctcc cttaatctgt
    13741 ttatcttttt attgtgtttc tactctatgt tttgcggtgc ttttatactg cctaccattt
    13801 tcgtatcttt gcctttcgtt taaaatttaa aatctatcat ctataatttc aaaagaatgg
    13861 aattagctaa aaatattagt cccgccgaaa ttgaagaaaa gtggtatgcg cattggttag
    13921 ccaaaaatta ttttcactcc gaaccggatg aacgcgagcc ttataccatc gtcattccgc
    13981 cacccaatgt aacgggcgta ttgcacatgg gacacatgct caataatacc attcaggatg
    14041 tattgatacg ccgtgcccgt atgcaggaaa aaaatgcctg ctgggtgccc ggcaccgacc
    14101 acgcctctat tgctactgaa gctaaagtgg tagccatgct caaagaacaa ggcatagata
    14161 aaaaacagct tacacgcgat gaatttttga cttatgcctg ggagtggaaa gaaaaatacg
    14221 gtggtatcat cttaaaacaa ttagaaaaac tcggtgcatc ctgcgactgg caacgcacaa
    14281 aattcacgat ggatgcatcc ctgagcgatg cggttacaga tgtatttatc gacttgtaca
    14341 acaaaggcta tatctaccgc ggagtgcgca tggtcaactg ggatccaaaa ggacttacag
    14401 cagtatctga cgaagaagta atccataaag aagtcaactc aaaactgttt tatattcaat
    14461 atgcaatttg cagtgatgac agtcaactgc cgactgccaa ctttgttacc attgccacca
    14521 cccgccccga aaccatcttg ggcgatactg ccatttgtgt gaatcccaac gatgaacgct
    14581 atcagcatct gaaaggcaaa tttgcttttg ttccacttat taatcgtaaa ataccgatca
    14641 tatttgatga atatgtagat atggaattcg gtacgggtgc tttgaaagta acacctgcac
    14701 acgatatcaa cgactataat ctcggcatca aacatcattt agaaaccatt gatattttaa
    14761 acgacaatgg aacattaaat gagaaagcac aaatttatgt aggtgaagac cgttttgccg
    14821 tgcgcaaaaa gatagttgcc gaattggaag aaaaaggctg tctggcaaaa gtagaagata
    14881 ttaaaaacaa agtgggctat tcggagagaa cagatgccgt tattgaacct aagttgtcga
    14941 tgcagtggtt tcttaaaatg gaagaacttt ccaaacctgc actcaaggca gtgatggaag
    15001 acagcgtaaa actgattcct gaaaaattca aaaataccta ccgccactgg atggaaaatg
    15061 tacgcgactg gtgcatatct cggcagttgt ggtggggaca ccgcattccg gcatggtatg
    15121 atgacaaagg caaaatcgca gtagccaaaa cagaagaaga agcccgtatc aaattgggta
    15181 ttcaagatat aactacaaca atcagacagg acgaagatgt actcgatacc tggttttctt
    15241 cgtggctgtg gcctatttca gttttcgatg gttttcaaga caaagacaat aaagacatca
    15301 actactacta cccaaccaac gatttaatta ccgcacccga aatcttgttt ttctgggtgg
    15361 cgcgtatgat tattgccgga tacgaatacc gcggtaaaaa gccgtttaac aacgtatatc
    15421 ttaccggaat agtgcgcgac aaacaaggac gtaaaatgtc gaagtcatta ggcaactcac
    15481 cggatccctt agatttgatt gcattgtatg gtgccgatgg cgtgcgtacg ggcatgttat
    15541 tttcttcccc ggcaggaaat gatttattat ttgatgaaaa actgtgcgag caaggcagaa
    15601 atttctccaa taaaatttgg aatgccttgc gccttgtaaa aggatgggaa attaattcag
    15661 acaacaagaa tcaagaatca agagacaaga caaatgcact tgaaactcag cattcaaaat
    15721 tctcttcttg gtttgaaaac aagttgcaac aggtaataaa aacaacggat gaactgtatg
    15781 cgcaatatcg tttatccgaa gtattgacaa cattgtattc ttttatttgg gatgattttt
    15841 gttcgtggta tttagagtat gtaaaaccgc ctttcggcga aaaaatcgac aaagccactt
    15901 acgaacaatc tgttcattat tttgaaacat taatgaaatt aatgcatccg tttatgcctt
    15961 ttattactga agaaatttat catcatttaa acgaaaacag aacggatgat attatcgttg
    16021 caaagtatcc gcttgctgcc gaaatacatc aacaagctat tgcagatggt gaaaaagcaa
    16081 aacaatttat ttcggctgta cgcgatatcc gaaacagaaa tggcatttca cccaaacaaa
    16141 cattggaagt ttgggtacaa actgaagata cttcatttta taatgccttt gaaaatgctc
    16201 tcgtaaaaat ggcgaatatt tcgtctgttc agtttacgga taaggaaata gaaaatgcag
    16261 taactgtttt attgaataaa gacaaactgt atatcatcac cggaatagaa gtaaataaag
    16321 acgatgaaaa acaaaaactg gaaaaagaaa tggagtatta taaaggcttt atcgcatcta
    16381 tagagaagaa actgagtaat gaaaagtttg ttcaaaatgc gaagcccgaa gtagtagaaa
    16441 acgaacgtaa aaaaattgcg gatggtcaag caaaattaaa agcattagaa gacagtttgg
    16501 caaaactata aaaacaacaa caatggctga taaaaaaaga tacacgataa ctgccgcatt
    16561 gccttatgcc aatggtcctt tgcatattgg gcatttggca ggtgcttata ttgctgcgga
    16621 tatttatgct cgctatttac gactcaaagg ggaagatgtc atttttgttt gtggtagcga
    16681 tgaacacggc gcagcaatta cgatcaaagc aaaaaaagaa aataaaaaac cgaaagaaat
    16741 aattgatgaa taccatactg taatcagaga tagttttgaa aaattcggca ttgcgttcga
    16801 tatctatcac cgcacctctg agccggtaca ttacgatact tcacaggaat ttttcagaat
    16861 attgaatgat aaaaacgagt ttgatattat tgaaagcgaa caatattatg acaaagaagc
    16921 caagcagttt ttagccgacc gttatatcac gggaacttgc ccaaaatgcg gcaatgaaag
    16981 tgcctacggc gaccaatgcg aaaaatgcgg ttcttcctta aatcctacgg acttaattca
    17041 tccgaaatca acgctaacaa acacctctcc tgttttgaaa aaaaccaaac act
//
LOCUS       DAOQII010000019        17047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1596_length_17047_cov_4.612641, whole genome shotgun sequence.
ACCESSION   DAOQII010000019 DAOQII010000000
VERSION     DAOQII010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17047
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_17047_cov_4.612641"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLF48_01875"
     CDS             complement(<1..254)
                     /locus_tag="PLF48_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNF48099.1"
                     /translation="MKKQVYNEHKQDETADNIEDELYEHYRFSIDKGKEPVRIDKYLA
                     ELIPNVSRNKIQQAAEAGNILVNEKSVKSNYKIRPLDTIQ"
     gene            410..3094
                     /locus_tag="PLF48_01880"
     CDS             410..3094
                     /locus_tag="PLF48_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF48100.1"
                     /translation="MNSFFAKRLILILILLNIVHAFATHNRAGEITYTHVAGFTYEFT
                     ITTYTKVSGASADADRPRLGISWGDGTFDSINRTSEVMLLADIKQNKYTARHTYSGPR
                     TYVVGMSDPNRIGDIINISNSVNILFYLEDSVKIYDPNLIGYNNSPQLLNPPIDNGVV
                     GQIFVHNPNAYDPDGDSLDFSIISPLQASGLAVPGYTPPNLIGPGPNNQISINPHTGE
                     LIWDTPQVVGIYNIAILVREYRSGVLIGTVIRDLQIFISDANNLPPKITMQEKICVVA
                     GTRIHLNITANDPNAGQIVTLTANGAPFLVPVSPATFVAAAPANPTSAIFDWQTSCDH
                     LTRNDYQVVVKATDNFTPLILSDLKTLIIKILAPAPQNPVAVLNTVNKTVLIKWDSLY
                     ACASNPKFLNFSVWRKKGCNVPIDTCSPDLAALGYQKIGTTNIYTFIDNTIRSGNEYS
                     YRITANFGDRSNAGIIINAFSGIASAEQCIIIPADLPLIYNVDVRSTSTTTGQIYIEW
                     SRPFAQRLDTISNPGPYVFKLYRAVGFSGTNFTLVTTKSFPTFSAINDTSYLDNNLNT
                     QNQPYTYKISFCARATDSLGTSEPASSVYLNALPQYEAILLSWNYSVPWINDSFVVYR
                     KNPGAATFDSITTVTTTTFRDTGLINDSLYCYVIKSVGAYPVAGLKEPLYNYSEEICA
                     RPSDTTKPCTPTLSVNNFCLDANLDTAEYRNYLTWQYQNTTNCFINDITKVYIYYASN
                     TTDSFKIIDSVLGNTFNNYTHILPYRSLAGCYAVQAQRQNGNLGSLSNTVCVDNCPLY
                     VLPNTFTPNGNGQNELYTPILPYRFVEKIDIKIYNRWGDLVFESTNPFIGWDGKDYKT
                     HKPVNSGVYYYLCDVYYQTVNGIAKLKTPLSGYIHLFR"
     gene            3118..5592
                     /locus_tag="PLF48_01885"
     CDS             3118..5592
                     /locus_tag="PLF48_01885"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF48101.1"
                     /translation="MQKTAGILVSFILFLCIQKNVFARTEKYDLSVQVADYTTGQGIA
                     YATYYDSLNQIQGTADSLGKFKLTIPKGSYTFEISSVGYQSLKRKINLTENTTISIGL
                     IADLTLGEITIAGQKTQNTVETNASGLTTLSTLSVQKLPSFFGEKDIMRAVLLTPGIQ
                     SGQEGARGIFVRGGSPDQNLMIYHKAPIFNASHIYGFLSVFPLEAISKMDIYKSFIPS
                     RFTGRLSSVLDIQPNYGNTDAWKGDYSISFITQKFHIEGPLKKDKTSFNLVLRDCHAG
                     AFVYPVSKTQYKKASGNDGSISYFFYDINAGIQHKINEHHTLNWSMYSGSDYYHFEEI
                     KNDINVPKDREVYKKLLKKLKWMNTNTTLEWNYQKQKLEADLSYHLSVYQLNPVQQSN
                     NQTTRISTGNISVSNADYKTLSRIIENGLHTTVKQHISKMHQIDYGMDMAYRFFIINK
                     VNRINKDKNNNVISNVEYKNDNVHVLDGFFFADYTLNLKDLVILKTGLQFYQYYDFQK
                     FRFYPLPRIEAIIHPRKEIDLRLSFMQTVQPLHLLTNNTGDIQNDVWVPATKKIAPET
                     AWQVSGGIQYNAEKGYTASIDAYYKQMKHLTEYRYGTFFMLDQLKWEDQLLSTGIGKA
                     YGIEFFAAKTRGQFTAWMKYSLGWSKRQFPELNQGDWYYYKYDRRHDFSIVLQYKMKK
                     HFDFTVSWTYGTGWRLTSPQSQYTTDYSIALIDLNNAQLTGSQGMALYWDKKNDYIMP
                     AYHHLDISMNYTKKAKRCTHQFNVSVYNVYNNFNVFTIYRDAATDANGNEYTKYVQLS
                     MFPVLPSLGYTVNFDIKTKKHKNEMP"
     gene            5579..6490
                     /locus_tag="PLF48_01890"
     CDS             5579..6490
                     /locus_tag="PLF48_01890"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNF48102.1"
                     /translation="MKCLNPFMFLVVLLAFFGISGCQKDFHFKNTDYTPKQVIVANFT
                     ANKQLAVNISKSKAVEDFTPIVFLDDCEVDLYENNIFIERMKYQLYDTLAQLGRYVSD
                     YKLKQDKTYYIVSKHPELGTASASEYLPKAIPFYASLANHADSGRTKTTAQITLTYHD
                     SAQFANLYYVAVLYRVLYLDTVNGVITNRDEWNLNIPLKSNLYRNPFNINVIFFNDDL
                     FDGYFHNLTFTFPSTYIKKAYQIQLLVEFTAIGKNYYDMFSQQLNLEENDFNQGSNEP
                     LNNISNIENGYGHFSSFNKNYKIFQIK"
     gene            complement(6488..6854)
                     /gene="ssrA"
                     /locus_tag="PLF48_01895"
     tmRNA           complement(6488..6854)
                     /gene="ssrA"
                     /locus_tag="PLF48_01895"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6957..7559
                     /locus_tag="PLF48_01900"
     CDS             6957..7559
                     /locus_tag="PLF48_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNF48103.1"
                     /translation="MMTALEQGILSGITVSLMIGPIFFGLVDLTITKGWRSGLAYVAG
                     IVVIDIVLIMLVENLLNRIDFHDIKQPMGIIGGILLLVFGVVIFMSKASTAHAPIEDI
                     KTLFQAFVKGIVINVLNPFVTVWWITMYTTFSINYDTIAEKIQFYFGILLMVFLFDLV
                     KIRFAYFLKQKLTIDKLIIVKKIAGMCLFIFGIVMIVRVM"
     gene            complement(7562..8437)
                     /locus_tag="PLF48_01905"
     CDS             complement(7562..8437)
                     /locus_tag="PLF48_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF48104.1"
                     /translation="MKTFIIGASGLVGNNCLDFFTQQEWNCIGTYFSYETDNLHFFDT
                     LNLENEKNFNIQAFQPDVIVHCGALTHVDYCELHPDESYEKTVVSTLNIIALAKTLNA
                     KLVFISTDYIFDGENGPYDEDAKPNPLSVYGRHKLEAEQAVIAANANNLVLRITNVYG
                     EEERGKNFVSRILQQIFEGKKLTLKLPLDQYATPINAFDIARCLYFLLNDNKCGVYNI
                     ASTDYMNRVQLAMTILKYFEFADYDLIPLTTLEIAAPAPRPLQGGLKNKKFMNEYPDF
                     RFTTVDEYVNSYVSE"
     gene            complement(8550..9593)
                     /locus_tag="PLF48_01910"
     CDS             complement(8550..9593)
                     /locus_tag="PLF48_01910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF48105.1"
                     /translation="MSHPSVAIVILNYNGKKWLEQFLPSVIEHNTYPNAEIIIADNAS
                     TDDSIAFLKAYYPQIRLVINEKNTGYAGGYNEALKQVEADYYVLLNSDVEASSSWIEH
                     LVQFMEDDKTYVACQPKILSFHEKNKFEYAGAAGGFIDKYGYPFCRGRIFDSCEIDTG
                     QYNTSGEIFWASGACMFIRADLFHLVGGFDADFFAHMEEIDLCWRLKNLGYKIGYCAE
                     STVYHVGGGTLNKSNPKKTYLNFRNNRKLLRKNLTENELKAIYNRRNQLDQLAALVAL
                     AKGNTDEANAIRQGLNDYKESIHKWDKKRTENERLQETMSIGKPNKVGIFNHSIVWKY
                     FVNGNKKFSDLKF"
     gene            complement(9622..10503)
                     /locus_tag="PLF48_01915"
     CDS             complement(9622..10503)
                     /locus_tag="PLF48_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF48106.1"
                     /translation="MKWLLTKVGLLWARLISLLPMNILFGLADFLYFLLSRVYAYRKQ
                     IIDINLMHAFMHKRSEELWYIRENYYRQLADFFVEVIKSITISKKELQKRMQLHHNSY
                     QLLREYEKLNQPIFVVLGHYNNWEWASLLAGTESQLPAIAVYQKPSNAAFDSFIKKNR
                     SRFGCSLIATQQLKSLYAALLKQASLVAFVADQTPVDTESAYWTTFLGMETPFYNGFD
                     KLAIRTNAIVLYTSIRKINRGYYEIAFETITAAAKAEQENQITEKFVRLLEKDIENRP
                     EYWLWSHRRWKRAGIKY"
     gene            complement(10905..12443)
                     /locus_tag="PLF48_01920"
     CDS             complement(10905..12443)
                     /locus_tag="PLF48_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNF48107.1"
                     /translation="MSNIEKFTQEMQAGYTFNGASILLGGAVLDKTPVSGVQVKAPLK
                     MFNRHGLVAGATGTGKTKTIQTLSEKLSDNGVSVLMMDIKGDFSGIAEKGVSNPKIEE
                     RVKVIGENWLPKAFPVELMTISNQDGVRLRSTVYEFGPVLISKILDLNDTQEGVVAMV
                     FKFCDDSNLGLVDLSDFKKVLNFLSNEGKGEFEKEYGAISPATVGTILRKVIELEQQG
                     ADLFFGELSFDVNDLLRKDENGCGYINIVRLTDIQDKPKLFSTFMLSLLAEVYAKFPE
                     RGDVEQPELIIFMDEAHLIFDNASKALQDQLEAIVKLIRSKGVGLFFCTQNPNDIPES
                     ILAQLGMKIQHALRAFTAKDRQAIKLVAQNYPETEYYKVEDLITELGIGEAFVTALNE
                     KGIPTPLVHCLMSPPQSRMDVLNDTEMAELIHSSKLYNKYKQTIDPESAFEILTDRIE
                     QMQQQQAQQAEEVAQKKSTTSRKAEKSTFDKVLNNTMTRQVARTAAREVTRGLLGVLK
                     GMFK"
     gene            12596..13498
                     /locus_tag="PLF48_01925"
     CDS             12596..13498
                     /locus_tag="PLF48_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNF48108.1"
                     /translation="MIRPPYLKKGDKIALTCPARWVETADIETALALFKSWGFEVVLG
                     DTVGKRDHQYGGSDDERRADFQHLLDDTAIKAIIAARGGYGTVRMFDDLNFDSYMNHP
                     KWIIGFSDITFLHAQQNFKLGVESMHAIMPLTIPGNSEASILSLKQALFGHKNTYQID
                     THPLNRTGNAEAMLVGGNLSILYAMLGTSSRLNFENCIIFIEDLDEYLYHIDRMMIAL
                     KRAGKLKQLRGLIVGGMSDMKDNTIPFGKTAEEIIREHVEEYQYPVCFNFPAGHIADN
                     RTLILGRKALLKVENNFVSFEQNA"
     gene            13542..13997
                     /locus_tag="PLF48_01930"
     CDS             13542..13997
                     /locus_tag="PLF48_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48109.1"
                     /translation="MKRLFLLTIVVLVSLMTFAKCKHKKKCCKKTTNTTTQTTSSTSG
                     EAAGRNPNEMWLGYDETQCANPWQFNWFKAPTDADLAGAVQGYLQGQGITVNEIRTSR
                     DKDMIHCEACTCLNGFHYYVKIPKDQEAKLKALKFHEIDTVPAIDNSRE"
     gene            14027..14446
                     /locus_tag="PLF48_01935"
     CDS             14027..14446
                     /locus_tag="PLF48_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48110.1"
                     /translation="MQKNHSLFLNNTNIMDDDSIDEYFLSETVSPKNWWEARRLSYNK
                     VCLVVGIIILIISSILLITSQENDFFSSGDIQIIWLMGLIYFIFGNAAYTLTWMVESI
                     FKINFSESSRQNFFVIFIILTLLIPAALFIMLLILIN"
     gene            14448..14807
                     /locus_tag="PLF48_01940"
     CDS             14448..14807
                     /locus_tag="PLF48_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48111.1"
                     /translation="MEQLEIMISARSWWEMRRLKYNLIVGTIGIIIVILLNKYVLKGG
                     ASFDIVFFLTSVGIAIIYALLCNLSYSLIWMLDDLYFKNELVNIHSPKRTFLLYGFCL
                     VSCIFPFGIFHLVKDLL"
     gene            14823..16535
                     /locus_tag="PLF48_01945"
     CDS             14823..16535
                     /locus_tag="PLF48_01945"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF48112.1"
                     /translation="MNIRYTLSFPEPHSHYLHVELQVNDIRQEQIALKMPVWTPGSYL
                     IREFQRNIDFVTATINEHNIRLKKRDKNTWILPLADATGFTVNYQLYCNELSVRTNFV
                     NSDFVLLNGAATFLYLEGYEKKAAEIIIQPYEKWDKCSTALHLAANNYWVRKSDNLDE
                     LVDSPILLGNHQSYTFEACGVKHELAMQGISNCNPEKLIADLQKMIAEEVKIFGDHPC
                     RHYTFINIHTASTYGGLEHAHSSVNMLPRWNFDTKNYAQSIGLLSHEYFHLWNVKRIR
                     PFELSTYNYDTESYTELLWFFEGVTSYYDDLVNRRAGILSKDEYLEIITSNLQVVMNT
                     PGIAVQTLAESSYDTWLKYYRKNENSNNATISYYTKGAIVAMLLDFMLMDATDSEKSL
                     DDVMYLLYAKYKSAPETGITEADILHALNETGGMDFSLYIQQFIHTTTSLPIEFYFDL
                     IGFDLLDQPKKESIFMGFSLSQKEGKFVVSEVINGFGAFAGGLNVDDEILTIDNFKIN
                     SDFTRLYEHKKINDTLTFLVSRQGEIKTLQLPTTADIRHQYRLVLQENLSEKQRKLLK
                     KWLR"
     gene            16576..16752
                     /locus_tag="PLF48_01950"
     CDS             16576..16752
                     /locus_tag="PLF48_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48113.1"
                     /translation="MRIIVFAIITILFIVSTHPASARNRYKRPKSCVSKVLLPGHSKP
                     MKCNKARKVKNAYF"
     gene            16782..16970
                     /locus_tag="PLF48_01955"
     CDS             16782..16970
                     /locus_tag="PLF48_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48114.1"
                     /translation="MKPFWKLFLLLLTVLFLAPALEAGHKSKKKSCPQKVYNPGAGSN
                     YKGHKCPKKKKIKLQKYY"
BASE COUNT         5449 a         3184 c         3009 g         5405 t
ORIGIN      
        1 atttgtatgg tatctaacgg acgaatttta tagttagatt ttaccgattt ttcattgacc
       61 aaaatatttc cggcttcagc agcttgttgt atcttattgc gcgatacgtt gggaattaat
      121 tctgccagat acttatctat gcgcaccggc tctttgccct tatctataga aaagcgataa
      181 tgctcataca attcatcttc gatattatcc gcagtttcat cttgtttatg ctcattatat
      241 acttgctttt tcatctctgc gataaagata aaaagataat gccttttttt aaaacgagct
      301 ataataaatt acaattttta tatacgacaa ttattattaa gttaaaaaaa tgtatctttc
      361 tcaatttcca ttatttccgt tatctttata gctgtttagt gtaaatatta tgaatagttt
      421 ttttgcaaaa cggctgatac ttattttgat tttattgaac attgttcacg catttgccac
      481 gcacaaccgt gcaggtgaaa ttacctatac ccatgttgcc ggatttacat atgagttcac
      541 tattactaca tataccaaag taagcggagc cagtgcagat gcagaccgcc caagattagg
      601 aatttcgtgg ggtgatggta cttttgactc tattaacaga acatctgaag taatgttgct
      661 tgccgatatt aagcaaaaca aatacacagc acgccacacg tattccggac ctcggacata
      721 cgtagtaggt atgagtgacc caaacagaat tggtgatatt atcaacatca gtaattcagt
      781 aaatattttg ttttatctgg aagattcggt gaaaatatac gacccgaatc ttattggata
      841 taacaactca ccgcaattat taaatccacc gatagacaat ggcgtagttg gacagatttt
      901 tgttcataat ccgaacgcct atgaccctga tggcgatagt ctggattttt ctattatttc
      961 tccattacaa gcttcaggac tggcagtacc cggctatacg ccaccaaatc ttattggtcc
     1021 aggaccgaat aatcaaattt caatcaatcc acacacagga gagcttattt gggatactcc
     1081 gcaggttgta ggtatttata atattgcgat tttggtaaga gaatatcgaa gtggcgtgtt
     1141 gattggtacg gtaatcagag atttgcaaat atttatcagt gatgcaaata atttaccgcc
     1201 taaaattacc atgcaagaaa aaatttgtgt ggttgccgga acacgcattc atttgaatat
     1261 tacggcaaac gatcctaatg ccggacaaat cgtaacactt acggcaaacg gagctccgtt
     1321 tttagttccg gtaagtcctg ctacttttgt ggctgccgca cctgccaatc cgacaagtgc
     1381 tatatttgac tggcaaacaa gttgtgatca cttgacacga aacgattatc aggttgtagt
     1441 taaagcaaca gataatttta cgcctctcat attatctgat ttaaaaacac tgatcattaa
     1501 aatattagca cctgccccgc aaaatcctgt tgccgtttta aatacggtaa ataaaacagt
     1561 attgataaaa tgggatagtc tgtatgcttg tgcatcaaat cccaaatttt tgaacttctc
     1621 ggtatggcgc aaaaaagggt gtaatgttcc aatagatacc tgctcccctg atttggctgc
     1681 tttaggctat caaaaaatag gcactactaa tatttatacc ttcatagaca atacaattcg
     1741 ttcaggaaac gaatatagct atcgtattac tgccaatttc ggcgacagaa gtaatgccgg
     1801 aattattatc aatgcctttt ccggcattgc atcagcagaa caatgtatta ttataccggc
     1861 agatttgcct ttgatttata acgttgatgt gcgaagtaca tctacaacta caggtcagat
     1921 atatatagaa tggtccagac catttgcaca acgcttagat accatttcca atccggggcc
     1981 ttatgtattt aaattataca gggcagttgg attttcggga actaatttca ctttagttac
     2041 aacaaaatca tttcctacat tttcagctat caatgatacc tcatatttgg ataataactt
     2101 gaatacgcaa aaccaacctt atacttataa aatttcattt tgcgccagag ctacagactc
     2161 attgggtact tcagaacctg cgtcatccgt ttatctcaat gcattgcctc aatatgaagc
     2221 cattctgtta tcatggaatt acagtgttcc atggataaat gacagttttg tggtgtaccg
     2281 caaaaatccg ggtgcagcaa cttttgactc cattactacg gtaactacta ctactttcag
     2341 agatacggga ttaatcaacg acagcttata ctgctatgtc attaagtctg ttggtgccta
     2401 tcctgttgcc ggattgaaag aacctttgta taattattca gaagaaatct gcgctcgtcc
     2461 aagcgacacc actaaacctt gcactccaac attaagcgtc aataatttct gtttagatgc
     2521 taacttagat acggcagaat atagaaacta tctgacctgg caatatcaaa atactactaa
     2581 ttgctttatc aatgacatca cgaaagtgta tatttattat gcatcaaaca cgacggactc
     2641 atttaaaatt attgacagtg tattaggaaa tacgtttaat aactataccc atatcctgcc
     2701 ttaccgctcg cttgccggat gttatgcagt acaagcacaa agacagaatg gaaatctggg
     2761 cagtctttcc aataccgtat gtgtggataa ttgtccgctt tacgttttac ccaacacatt
     2821 tacgccaaat ggcaacggtc aaaatgaatt atatacccct attttgcctt atcgttttgt
     2881 agaaaaaata gatatcaaaa tttataaccg ttggggtgat ttggtttttg aatccacaaa
     2941 tccatttatt ggttgggatg gcaaagacta taagacacat aaaccggtaa atagtggggt
     3001 atactactat ttatgtgacg tttattatca gacagtaaat ggcatagcga aattaaaaac
     3061 tccgttatca ggttatattc atttgttcag ataataaagc cgagtggaat tatcaccatg
     3121 caaaaaacag ccggaatact tgtatctttt atcttgtttt tatgtattca aaaaaatgtt
     3181 tttgcccgaa cagaaaaata tgacctaagt gttcaggttg ctgattatac taccggacag
     3241 ggaattgcat atgctactta ttatgattcg ctaaatcaaa tacaaggcac agcagattct
     3301 ctgggcaaat tcaagctgac gataccaaaa ggcagttata cttttgaaat ttcttcagtg
     3361 ggctatcagt ccttaaaaag aaaaataaat ttaactgaaa acacaactat cagcatcggt
     3421 ttaatagctg acttaacact gggagaaatt accatagccg gacagaaaac gcaaaataca
     3481 gtagaaacaa atgcttccgg tttaactaca ctttctacat taagcgtaca gaagcttccc
     3541 tccttttttg gggaaaaaga tattatgcga gctgtattac ttacccccgg tatacaaagc
     3601 gggcaagaag gtgccagagg tatttttgtt cgtgggggaa gtcccgatca aaacttgatg
     3661 atataccaca aagctcctat tttcaatgct tcacatatct atggttttct ttccgtgttt
     3721 ccattagaag ccatcagcaa aatggatatt tataaatcat ttatcccgtc tcgttttacc
     3781 ggcaggttat cttccgtatt ggatatacaa cccaactatg gaaataccga tgcatggaaa
     3841 ggtgattaca gtatcagttt tatcacccaa aaatttcata ttgaaggtcc gttaaaaaaa
     3901 gataaaacat cattcaattt agtactgcgc gattgtcatg caggtgcatt tgtttatcct
     3961 gtttctaaaa cgcaatataa aaaagcatcc ggcaatgacg gcagtatcag ttatttcttt
     4021 tatgatatca atgccggtat tcagcataaa ataaacgaac accatacctt aaactggagc
     4081 atgtattcgg gcagtgatta ttatcatttt gaagaaataa agaatgacat caacgtacca
     4141 aaagacagag aagtatataa gaagctgctg aaaaagctga agtggatgaa tacgaacaca
     4201 acactggaat ggaactatca gaaacaaaag ttagaagcgg atttatctta tcatttaagc
     4261 gtctatcagc ttaatcccgt tcagcaatcg aataaccaaa ccacacgcat ttctacaggc
     4321 aatataagtg tttccaatgc agattataaa acgttgagcc gcattattga aaacggatta
     4381 catacgacag ttaaacagca tattagcaaa atgcatcaaa ttgactacgg tatggacatg
     4441 gcttatcgct tttttattat taacaaagta aacagaatca acaaagacaa aaacaataat
     4501 gtgataagca atgttgaata taaaaatgac aatgtacacg tattggatgg tttctttttc
     4561 gcagattata cattaaacct taaggattta gttattctta aaactggatt gcaattttac
     4621 caatactatg attttcagaa attccgtttt tacccactcc cccgcataga agcaattatt
     4681 catccgcgaa aagaaataga tttgcgctta tcatttatgc aaacggtaca acccttacat
     4741 ttgctgacca ataatacggg cgatatccaa aatgacgtgt gggtgccggc caccaaaaaa
     4801 atagcacctg aaacggcatg gcaggtttcg ggtggcatac aatataatgc tgaaaaagga
     4861 tacaccgcca gtattgatgc ttattacaaa cagatgaagc atttgacaga atatcgctat
     4921 ggcactttct ttatgctcga ccaattgaaa tgggaggacc aattgcttag taccggaatc
     4981 ggtaaggcat acggtattga attttttgct gcaaaaacaa gagggcaatt tacggcctgg
     5041 atgaagtata gtctagggtg gagcaaacga caatttccgg aattaaacca aggagattgg
     5101 tattattata aatatgacag acgacatgat ttttcaatcg tgttacaata taaaatgaaa
     5161 aaacattttg attttacagt ttcctggacg tatggcacag gttggcgttt aacttcaccg
     5221 caatcgcaat ataccactga ttattctatt gcattgattg acttaaacaa tgcacaacta
     5281 accggatcgc aaggcatggc tctttattgg gataaaaaaa atgactacat catgccggcc
     5341 tatcatcatt tggacatcag catgaactat actaaaaaag ccaaacgatg cacgcatcag
     5401 tttaatgtaa gtgtttataa tgtgtataat aactttaatg tatttaccat ttacagagat
     5461 gcggcaacag atgccaacgg gaatgagtat accaaatatg tacaactaag tatgtttccg
     5521 gtgctgccct ctttgggcta caccgtgaat tttgatatta agactaaaaa acacaaaaat
     5581 gaaatgcctt aatccattca tgtttcttgt tgttttgtta gcattctttg gtataagtgg
     5641 atgtcagaag gattttcatt ttaaaaatac tgactatacg ccaaaacaag taattgttgc
     5701 caattttact gcaaataaac aactggcagt aaatatcagt aagagcaaag cagttgaaga
     5761 ttttacgcca attgtatttt tagatgactg tgaagtggat ttatatgaaa acaatatttt
     5821 tatcgagcga atgaaatatc agttatatga tacactggct cagctaggca gatatgtttc
     5881 cgattacaaa ctaaaacaag ataaaaccta ttatatcgta tctaagcatc ctgaacttgg
     5941 aacagcttca gcaagcgaat atttacctaa agcaatacca ttttatgctt ctctggccaa
     6001 tcatgctgac agtggcagaa caaaaaccac agcacaaatt acattaacct atcatgattc
     6061 ggcacaattt gccaatttgt attatgtagc tgttctttac agagttctct atctcgatac
     6121 agttaatgga gtaattacga acagagacga atggaacttg aacattccac taaaaagcaa
     6181 tctctataga aatccattta atataaatgt aatatttttt aatgatgatt tatttgacgg
     6241 atattttcat aatctgacat ttacctttcc cagtacgtat ataaaaaaag cataccagat
     6301 tcaattactt gtagaattta cagcgattgg aaaaaattat tatgacatgt tcagtcaaca
     6361 attaaattta gaggaaaatg actttaatca aggttctaat gaaccattaa ataacatttc
     6421 caatattgaa aatggatatg gtcatttttc aagttttaat aaaaactata agatatttca
     6481 aataaaatag tggagctggt gagatttgaa ctcacgtccg gacaaaatgc ctatacgctt
     6541 tctacatgct tagtgttaca ttgattttcg tgtaaaagca ggttgcacca cctaccgact
     6601 attaccttag tttctatttt tcatcgctca gttgaaaccc aagagcaact atctctatta
     6661 gatgatgtac tgacgaagaa catagagcaa attctttcgg gtacataggc cggttaatta
     6721 cctgaattaa gcagccaggg caagagtatt attgccatat aaaaaaagtt aatgcattga
     6781 gattatagag ccactcgcac aaagctctgc atgcttactt atctgctgtt tcgccgtcaa
     6841 agcctgtcag ccccaatatg tcaaagagtt attactaaaa cgataccgtt ttaatattag
     6901 ttccgcaaag gtagaatatt tttttcaata gcgatttaaa aaatgtaatt tgcgaaatga
     6961 tgacagcatt agaacaaggt attttatccg gtatcacagt aagcttaatg attggtccca
     7021 ttttttttgg cttagtcgat ttgacaatta ccaaaggctg gcgctcaggt ctggcatatg
     7081 tagccggcat tgttgttata gatatcgtat taattatgtt ggttgaaaat ctgctaaacc
     7141 gtatcgattt tcacgacatc aaacagccca tgggcataat cggtggtatt ttattgcttg
     7201 tattcggtgt tgtcattttt atgtcaaaag cttcaacggc acacgctcct attgaagata
     7261 taaagacctt atttcaggct tttgtaaaag gcattgttat taatgtattg aatccgtttg
     7321 taaccgtttg gtggattacc atgtacacta cattttcaat aaattatgat accattgctg
     7381 aaaaaattca gttttatttc ggcatattac ttatggtctt tttatttgat ttagttaaaa
     7441 tccgttttgc ctattttctc aaacaaaaac tgacgataga caaactgatt atcgtgaaaa
     7501 aaatagccgg catgtgcttg tttatttttg gtatcgtgat gattgtaaga gtaatgtaat
     7561 gttattctga aacataactg ttcacatact catcaactgt cgtaaaacga aaatccggat
     7621 attcattcat aaacttttta tttttcaatc cgccttgtaa tggtcgcgga gcaggagctg
     7681 caatttcgag agtggtcaat ggaattaaat cgtagtctgc aaactcaaaa tattttaaga
     7741 tggtcatggc caactgtact ctgttcatat aatccgttga ggcaatattg tacacgccac
     7801 atttattatc gttcaataaa aaataaagac aacgtgcaat atcaaatgca tttatcggcg
     7861 tagcatattg gtctaaaggc aacttcagcg tcagtttttt cccttcaaaa atttgctgaa
     7921 gtatacgact gacaaaattc ttacctctct cctcttcgcc ataaacattg gtaatacgca
     7981 acaccagatt atttgcattt gctgctataa cggcttgctc cgcttccagc ttgtgtctgc
     8041 catatactga aagaggattc ggcttagcat cttcatcata aggaccattt tcgccatcga
     8101 aaatataatc cgttgaaata aaaaccaact tggcatttag cgtttttgcc aatgcaatta
     8161 tatttaacgt ggacaccact gttttttcat aactttcatc cggatgtagt tcgcaataat
     8221 cgacatgcgt aagtgcaccg caatgcacga ttacatcggg ttgaaatgct tgtatattaa
     8281 agttcttttc attttctaaa ttaagggtat caaaaaaatg caggttatct gtttcataag
     8341 aaaagtaagt gccgatacag ttccattcct gttgagtaaa aaaatcaagg cagttgttgc
     8401 ctacgagtcc cgatgcaccg atgataaaag ttttcatata gattgattgg tgagtagtta
     8461 ttggtaattg gttattagtg attagtgatt ggtaaattgg tacattatta aattgataca
     8521 ttacaaatta gcatacaaat atttacatct taaaacttta aatcactgaa ttttttattg
     8581 ccgtttacaa aatatttcca tactatacta tggttaaaaa tgcccacttt atttggcttt
     8641 cctattgaca tcgtttcttg caagcgttcg ttctcagtgc gttttttatc ccatttatga
     8701 atactttctt tatagtcatt cagtccttgc ctgatggcat ttgcctcatc tgtattgcct
     8761 ttagccagag caaccagtgc agccaattgg tctagttgat ttcgtctgtt ataaattgct
     8821 ttcagttcat tttctgttag atttttgcgc aatagtttac ggttgtttct aaaattgaga
     8881 tatgtttttt tagggtttga tttattaagt gttccaccgc ctacatgata gactgtgctt
     8941 tcggcacaat agccaatttt atatcctaaa tttttaagcc gccagcataa atcaatttct
     9001 tccatgtggg caaaaaaatc ggcatcaaag ccaccaacca gatgaaacaa atctgcacgg
     9061 ataaacatgc aggcaccgct tgcccaaaaa atctccccac tggtattata ttgtccggta
     9121 tcaatttcac aagaatcaaa aatgcgaccg cgacagaaag gataaccgta cttatctata
     9181 aatccacctg ccgcacctgc atattcaaat ttattttttt cgtggaatga aagaattttc
     9241 ggctgacaag caacataggt tttatcgtct tccatgaatt gcaccaagtg ttcaatccag
     9301 cttgagctag cttccacatc cgagtttaac aacacgtaat aatcagcttc aacctgtttt
     9361 aaggcttcat tatatccacc tgcatacccc gtattttttt catttataac aagacgaatc
     9421 tgtggatagt atgcttttaa aaatgcaatt gaatcgtcag tagaagcatt atccgcaatt
     9481 attatttcag cattgggata ggtattgtgt tcaataacag atggcaaaaa ttgttctaac
     9541 catttttttc cgttataatt gagaataaca atagcaactg atggatgact cactttgcaa
     9601 aaatacaaaa atcatgtgac ttcaatattt tatacccgca cgtttccatc gtcgatgact
     9661 ccatagccaa tactcggggc ggttttcaat atccttttcc aacaagcgca caaatttttc
     9721 agtaatctga ttttcctgtt cagctttagc tgctgctgta atcgtttcaa aagcaatttc
     9781 gtaatagccc ctgtttatct ttctgataga ggtataaagt acgatagcat tagtacgaat
     9841 tgccagttta tcaaatccgt tgtaaaaggg tgtttccata cctagaaatg tagtccagta
     9901 agcagactca gtatcaaccg gagtttgatc ggctacaaaa gccacaagtg aagcctgttt
     9961 tagcaaggca gcataaagcg atttgagctg ctgggtggca ataagtgaac atccaaagcg
    10021 tgagcggttc tttttaataa atgaatcaaa ggcagcatta gatggttttt gatacacagc
    10081 tatagccggc aactgacttt ccgtacctgc taataaagat gcccattccc agttattata
    10141 atgtccgaga actacaaaaa taggttgatt gagtttttcg tattccctca aaagctgata
    10201 gctgttatgg tgcaattgca tacgtttttg cagttctttt ttgctgatgg taatagattt
    10261 aataacctct acaaaaaaat cggctaactg tctgtaatag ttttcacgaa tataccagag
    10321 ttcttcgctg cgtttatgca taaaggcatg cattaaattg atatcgataa tttgtttgcg
    10381 ataagcataa acacggctta aaaggaaata aagaaaatca gccagaccaa ataagatgtt
    10441 catgggtaag agcgaaatca acctcgccca cagtaatccg actttagtca gcaaccattt
    10501 cattttttca atgttgaatc aatgttgatt agttaaaaat atgatgaaag cagttttaga
    10561 atttgaatct accattagta tttattgtat ttaacattat actcttttta cgttaagaac
    10621 tgatttgtat tttttacaat gattgcagta aattaaaatt tttctttcca cctcccccta
    10681 ccccctcctt tttaaggagg gggattaaag ggggaggtat ttattattat aaaataattt
    10741 ttttaacata ttatttttta agcttacaaa attgaataaa aaatgataaa actgcttctt
    10801 tatttaaaac tatattgaca agttcaatat tgatgtagca tttaaattat cttcattctt
    10861 tattttgcca actaagtacg caaacttcca actactcccc ttttctattt aaacatacct
    10921 ttcaatacgc ctaacaagcc tctggtaact tccctcgcag cagtgcgtgc cacctgacgt
    10981 gtcatagtat tattcaatac tttatcgaaa gtagattttt ctgcttttct gcttgttgtg
    11041 gatttctttt gtgccacttc ttccgcttgt tgagcttgtt gttgttgcat ttgttctatt
    11101 ctgtctgtta aaatttcaaa tgcactttcc ggatctatgg tttgtttgta tttattatac
    11161 aatttagatg aatgtattaa ttcagccatt tctgtatcat tcaatacatc catacgagat
    11221 tgtggcgggc tcatcagaca gtgtactaat ggcgttggaa tacctttttc attcaatgcc
    11281 gttacaaagg cttcaccaat acccaactcc gttatcaaat cttcgacttt gtaatattcc
    11341 gtttcgggat agttttgtgc cactaattta atagcctgac ggtcttttgc cgtaaaggcg
    11401 cgcaaggcat gttgtatttt catacccaac tgtgccaata tgctttccgg aatatcattc
    11461 gggttttgcg tgcaaaaaaa caaacctaca cctttcgaac gaatcaattt gacaatagct
    11521 tccagttgat cctgtagtgc cttgctggca ttatcaaaaa tcagatgagc ttcatccata
    11581 aagataatta attcaggctg ttccacatct ccgcgttcag gaaatttggc ataaacctca
    11641 gccaataaac ttagcataaa cgtggaaaac aatttcggtt tatcttgtat atctgtcaag
    11701 cgaacaatat tgatatagcc gcatccgttt tcatctttgc gcagcaaatc attgacatca
    11761 aaagacagtt cgccaaaaaa caaatccgct ccttgctgtt ccagttcaat tactttacgc
    11821 aaaattgtac ccactgtagc cggagaaatc gcaccatatt ctttttcaaa ttcgcctttt
    11881 ccttcattag aaagaaagtt gagtaccttt ttaaaatccg acaaatcgac cagccctaag
    11941 ttactgtcat cacaaaattt aaataccatg gccaccacgc cttcttgcgt atcattaagg
    12001 tctaaaattt ttgaaatcaa aaccggccca aattcatata cggtagaacg caaacgaact
    12061 ccatcttgat tagaaatagt catcaattcg accgggaatg cttttggtaa ccagttctca
    12121 cctatcactt ttacccgctc ttcaatttta ggattagaca cccctttttc ggcaatacca
    12181 gaaaagtcgc ctttgatatc catcatcagg acagaaacgc cattatcgga caatttctca
    12241 gataaagttt gaattgtttt cgtttttccg gtacccgtag ccccagccac tagaccgtga
    12301 cggttaaaca ttttcaacgg agcttttacc tgaacgccag atacgggagt cttatccaat
    12361 acggcgccgc ctaagagtat ggatgcacca ttaaaggtat atcctgcttg catttcttgt
    12421 gtgaattttt ctatgtttga catgttcatt aaaataacag cactaaaata acttaatttc
    12481 tcttgctttt attcacagct aaaataaaat gattattttt taatttgata gctacgtccg
    12541 gacaggtatt cttccttatt tttttattct gtcttattcg ttaattttac ggctaatgat
    12601 acgtcctcct taccttaaaa aaggcgataa aatagcgctc acttgccccg cccgctgggt
    12661 ggaaacagcc gatatagaaa cagccttagc cctattcaaa tcctggggtt ttgaagtagt
    12721 tttaggcgat actgtcggta aaagagacca ccagtatgga ggaagtgacg atgagcgcag
    12781 agcagatttt cagcatttat tagatgatac tgctatcaag gcaataattg ccgcaagggg
    12841 cggttatggt acggtacgca tgttcgatga tttaaatttt gacagctata tgaatcatcc
    12901 gaaatggata attggctttt cggatattac atttcttcat gcccagcaaa attttaaatt
    12961 gggagttgaa tccatgcatg ccattatgcc actgacgata ccgggaaaca gcgaagccag
    13021 catactgagt ttaaaacaag ctttattcgg tcataaaaat acttatcaaa ttgacacgca
    13081 tccgctgaac agaacaggga atgcagaggc gatgctggtt ggtggaaatt tgtctatact
    13141 atatgctatg ctgggtacga gttctagatt gaattttgaa aattgtataa tatttattga
    13201 agatttggac gaatatcttt atcatatcga ccgaatgatg attgccttga agcgagcggg
    13261 aaagctgaag caattgcgag gattaatcgt agggggcatg agtgatatga aagacaatac
    13321 aataccattc ggcaaaacgg cagaagaaat tatacgggaa cacgtggagg aatatcagta
    13381 tcctgtttgt tttaattttc cggcaggaca tattgctgat aaccgtacac ttattttagg
    13441 aagaaaagcc ttattaaaag tggaaaacaa tttcgtaagt tttgaacaaa atgcataaaa
    13501 gcatggcttg atttttgaat tatcttaaaa aaacagaaat aatgaaaaga ctttttttat
    13561 tgacgattgt tgtacttgta tctctaatga catttgcaaa gtgcaaacac aaaaagaaat
    13621 gttgtaaaaa aacgactaat acgactaccc aaacaacttc ttctacttca ggtgaagctg
    13681 cagggcgcaa tcccaatgag atgtggctgg ggtatgatga aacacaatgt gctaacccgt
    13741 ggcagtttaa ctggtttaaa gcaccaacag acgcagattt ggctggagcg gttcaaggtt
    13801 atttgcaagg ccaaggaatc accgtcaatg agatccgcac ttcaagagat aaagacatga
    13861 tacattgtga agcttgtacc tgcttaaacg gatttcacta ttatgtaaaa atacccaaag
    13921 atcaggaagc aaaattgaaa gcgcttaaat ttcatgaaat tgacacggtg cctgccattg
    13981 ataacagcag agaatgagtt aaaaatcatt aacatacatt tgcaatttgc aaaagaatca
    14041 ttccttattt ttgaacaaca caaatattat ggatgatgat tcaatagacg aatattttct
    14101 ttcagaaaca gtcagtccta aaaactggtg ggaagcaaga cgactatctt acaacaaagt
    14161 ttgtttggtt gttggcatca ttattctcat catttccagc atattattaa ttaccagtca
    14221 agaaaatgac tttttctcaa gtggtgatat ccaaataatc tggctcatgg ggcttattta
    14281 ttttattttt ggaaatgccg cttacaccct cacctggatg gttgaatcta tttttaaaat
    14341 taatttttct gaatccagca ggcaaaactt ctttgtaatt ttcataattt taacattgct
    14401 aattccggca gctctcttta tcatgctgct gatattaata aattagtatg gaacaactcg
    14461 aaattatgat atctgcccgc tcatggtggg aaatgcgtcg cttgaaatac aatctgattg
    14521 taggtacaat aggcattatt attgttattt tgctgaataa atatgtactg aaaggcggtg
    14581 ctagttttga tattgtattt ttccttactt ctgttggtat tgccattatt tatgccttac
    14641 tctgtaattt atcttattcg ttgatatgga tgctggatga tttgtatttt aaaaatgaac
    14701 tcgtcaatat acattctcct aagcgcacat ttttgctcta cggcttttgt ctggtttcct
    14761 gtatttttcc attcggcatc tttcacttag taaaagattt gctgtaattt attttattta
    14821 tcatgaacat acgttatacc ctgtcttttc ctgaacctca ctcccactat ctgcatgttg
    14881 aactgcaagt aaacgatatt cggcaagagc aaattgcatt aaaaatgccg gtgtggacac
    14941 cgggcagtta tcttatccgt gaatttcagc gcaatatcga ttttgttacc gcaaccataa
    15001 acgagcataa catcagacta aaaaaacgag ataaaaacac ctggatacta cccttggcag
    15061 atgcaactgg ttttacggta aattaccaac tctactgcaa tgaactgagt gtgcggacaa
    15121 acttcgtgaa ctcagatttt gttttgctga atggagcagc aacttttctt tatcttgaag
    15181 gatatgaaaa aaaagcagca gaaattatta tccaacctta tgaaaaatgg gataaatgtt
    15241 caactgctct ccatcttgca gcgaataact attgggttag aaaatcagat aatctggatg
    15301 agctcgtaga ttcccccatt ttgttaggca atcatcaatc ttatacattt gaggcatgcg
    15361 gcgttaaaca tgagttggcc atgcaaggca tcagcaactg taatcctgaa aaattaatag
    15421 ccgacctgca gaaaatgatt gcagaagaag ttaagatatt cggtgatcat ccttgccggc
    15481 attatacctt tatcaatatt catactgcct ctacttacgg cggtttggag catgcgcatt
    15541 catctgtcaa tatgctgccg cgctggaatt ttgataccaa aaattatgcg cagtctatcg
    15601 gtttattatc tcatgaatat tttcacttgt ggaatgtaaa acgcatccgt ccttttgaat
    15661 taagtactta taattacgat acggaaagct atacagaact gctttggttt tttgaaggtg
    15721 tgaccagtta ttacgatgat ttggtgaacc ggcgagctgg cattttgagc aaagatgagt
    15781 atctcgaaat tatcacatcc aatttacaag ttgtgatgaa tacaccggga atagccgtgc
    15841 aaacactggc agagtcgagt tatgatacct ggctgaaata ctaccgcaaa aatgaaaaca
    15901 gcaataatgc taccatcagt tattatacaa aaggagctat tgtggctatg ctattagatt
    15961 ttatgctgat ggatgccaca gacagcgaaa aaagcctcga tgatgtaatg tatttactct
    16021 atgctaaata taaatcagca ccggaaaccg gcattacaga agctgacatt ttgcatgcac
    16081 tgaatgaaac cggaggcatg gatttcagct tatacatcca acaatttatt catacgacaa
    16141 catctttgcc gattgaattc tattttgatt taatcggatt cgatttattg gatcagccta
    16201 aaaaagaaag catttttatg ggattttcgc taagtcaaaa agaaggaaaa tttgttgtat
    16261 ctgaggtgat aaatggattt ggtgcttttg ccggaggatt gaatgttgac gatgaaatac
    16321 tgactattga taattttaag ataaacagtg actttacacg tctttatgag cacaaaaaaa
    16381 ttaatgacac actaaccttt ttagtatcac gtcaggggga gataaaaacg ctgcaacttc
    16441 caaccacagc agatatccgc catcaatatc gcttggtatt gcaagaaaat ttatcagaaa
    16501 aacaaagaaa gctcttaaag aaatggctta gataatttaa catttaagca ttgagatcta
    16561 tagtattttt gtaatatgag aattattgta tttgccatta ttactatctt atttatagtg
    16621 agtactcatc ctgcatcagc gagaaacaga tataagcgtc cgaaaagttg cgtatctaaa
    16681 gtattattac ccggacattc caagcctatg aaatgcaaca aagccagaaa ggtaaaaaat
    16741 gcttattttt aaattggaat tcaaatccaa aaaaaacaac tatgaaacca ttttggaaac
    16801 tctttctttt actactgaca gtactatttt tagcaccagc actagaagcc ggacataaaa
    16861 gcaagaaaaa aagctgtccg caaaaagtgt acaatccggg tgcaggctca aactacaagg
    16921 gacataaatg tcctaagaag aaaaaaatca aattgcagaa atactattaa cctgatttcg
    16981 ggatgtcagt tccaatttaa aaatagttga ttttttatct ataacctcct acttttaatt
    17041 ccacctc
//
LOCUS       DAOQII010000020        16881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1618_length_16881_cov_5.080530, whole genome shotgun sequence.
ACCESSION   DAOQII010000020 DAOQII010000000
VERSION     DAOQII010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16881
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_16881_cov_5.080530"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLF48_01960"
     CDS             <1..406
                     /locus_tag="PLF48_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNF48115.1"
                     /translation="SKAALICTTFPDMNGQFYPAAINFANTDVYEYSTKLLFQFLHRT
                     NAYLIFIWVIFLFFITKKWNDIFEFQRTRWLLLIMVGIQLLLGIFTLLNSQGSIPVVL
                     GVLHQLGAFLLLIFSIEGFYFISYKGKEQKVV"
     gene            446..1018
                     /locus_tag="PLF48_01965"
     CDS             446..1018
                     /locus_tag="PLF48_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48116.1"
                     /translation="MKIKIAHISNLSDARFFSAFGVDYFGFSLDSLNPNAVTVNEVKD
                     ITQWLYKPILVGEFGIHQNQAEIAYLVQQLSLQEIQIPFAHPDKDLMDFSKFIQIDYN
                     EIQKVTGSADYFILSLNNKQLADKAVLDFIQQHKVFLEAPVEPEMLNAWLTIVRPYGI
                     QIRCQRESEDCLFNTDYYAEVLEIIEQHTT"
     gene            1038..2477
                     /locus_tag="PLF48_01970"
     CDS             1038..2477
                     /locus_tag="PLF48_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48117.1"
                     /translation="MSTPNRGSIFLLTIVLFVFFWIYNFPFFWKLPPQGSHIWRQSDC
                     MAMVNNFKENKLPFFQPETYNLQSEQGKCAGEFPILYYIAAKFSDPTYALRVIDSFIF
                     ILGVIAVFFIAFYFLQDELLALLPSLLLFTSPLLVFYGNNFLSDVPALSFAYIGWAFF
                     FYSEGRKSKVIFLLALVFFVFSVLLKASQLLNFAILFIYLFAYKKEAKFSSLFSLCLF
                     ALIPVAWYAYAKWYNAQHHDTYYFLGIYPIWKLSVYEIGLCIWRMTMSWGKNYFWPTT
                     SVLLIIASALLWKHKNKLHPELRLLLLYSFLFTTLYILLYFQRMIRHEYYYVAFYVFV
                     LFFLISLLLLYKKFYAENIFSHTALFLFLIPNLVFCNYYIHEKLKDNRPNAVLSSSGF
                     QYFLEKNGVTKDKMVLILPDISTNITLNQIHRKGYTAYNTIDEVLKQHKADFILLGDS
                     SLITNMRKYIATDTAATYREMFLYRLKKE"
     gene            2787..4085
                     /gene="serS"
                     /locus_tag="PLF48_01975"
     CDS             2787..4085
                     /gene="serS"
                     /locus_tag="PLF48_01975"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNF48118.1"
                     /translation="MLTIEQLKQDTAFAKERIAHKNFKDIHLVDEILEMEEERKKLKT
                     SLDNYLSQRNTISKEIGALMGKSKGLSGDALAEAKAQEEKLKAQIADLKIQIDEAESQ
                     HDKIVAAQFEKLVLLPNFPNAIVPKGNSAEDNEITFSVSEFPSLPESALPHWELAKKY
                     DLIDFELGNKITGAGFPVYKGKCAQLQRALIAFFLDKATEAGYAEVMVPHVVNRDSGF
                     GTGQLPDKEGQMYHIGIDDLYLIPTAEVPITNLYRDVIIDEKNLPIKNVGYTPCFRRE
                     AGSYGSDVRGLNRLHQFDKVEIVRIEKPENSYKALEEMLQHVEGLLKSLKLPYRILRL
                     SGGDMTFSSALTYDFEVYSAGQKKWLEVSSVSNFETFQANRLKLRLKDNDNKKRLAHT
                     LNGSALALPRIMASILENYQTADGITIPEVLVPYTKFEKI"
     gene            complement(4093..5133)
                     /locus_tag="PLF48_01980"
     CDS             complement(4093..5133)
                     /locus_tag="PLF48_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48119.1"
                     /translation="MRLHTYIIGFILLLISCKKEAKVASGQLDNYYFVKVGDVALPVR
                     VCGNINSDIAIVFNHGGPGGSAQGERAYAYWKEIEKYYKVVYWDQRGSGLTQGNTDPG
                     TITIEQFSEDLDNIVDFTKQVVKADKIFIHGISWGGGLTTYYLLDTLHQKKVKGAIVE
                     APAYDLKHGIMQLSVNWLLQRADSMLALHVNDAYWQNVKDIYAAYPAITNVHMFQQHL
                     SFLGQVNGIVYNTSNLAAQAITLPKYESFVGSYNSMFTINHLSYNQASLFEMDLTDQL
                     YKIKVPLLLIWGRKDGLLPFDNLAQKYADNYGGSDIKFEPYRYDVSAHLPHAEQYQEF
                     QIDALKFIELHK"
     gene            complement(5136..5810)
                     /locus_tag="PLF48_01985"
     CDS             complement(5136..5810)
                     /locus_tag="PLF48_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48120.1"
                     /translation="MHSFVRYLFLCCFSILCTYAAIAQEQTDYKEIIKIDGLKFKKNI
                     IAVNALADVEFVKPMQISYGLSISGAYRFFYKPKLNKGTSEYNPKIIERELFLRPAFG
                     YIYRKRYNTGIFFIPELTYRHTFNVGIFSEVSFDAGYMYTKLNAPVYERQADGTFKKV
                     SFGYHNLLLGGKVDFGYDFSKKTDAPLDIHLGCNLLYRYPNNGRWVRRLIFEVGISYV
                     IRKKSE"
     gene            complement(5856..7457)
                     /gene="pckA"
                     /locus_tag="PLF48_01990"
     CDS             complement(5856..7457)
                     /gene="pckA"
                     /locus_tag="PLF48_01990"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNF48121.1"
                     /translation="MNEQGVKNPSADLSKLGFSKTTALWNLSRENLIQKTLDLDLGVL
                     NDTGALCVNTGKFKGRSPADKFTVKDELTENAVDWNKINQPFSPEKFDALQEKVVAYL
                     DSLQQIFVRDVKACAAPEYTLGIRVVTETPWANLFADNMFLRLDDAQIQTAEPEWLIL
                     NAPGFLADATVDGTRQGNFSIINFTKKIVLIGGSAYTGEIKKGIFTVLNFELPHLKNV
                     LSMHCSANIGDGGDTALFFGLSGTGKTTLSADPNRKLIGDDEHGWSDTSVFNFEGGCY
                     AKCVDLTEEKEPDIFRAVNHGALLENIEFYPGTNTVDYANISQTENTRVSYPIYHIHN
                     IAVPSVGGVPKNIFFLSYDAFGVLPPISKLTSAQAMYLFISGYTSKVAGTEEGVVEPQ
                     TTFSACFGAAFLPLHPAKYAAMLGNKLAQSHANVWLINTGMTGGAYGVGKRMSLKYTR
                     ALITAALSGELDAVSYETLPIFDLQIPVTCPGVPAEILNPRNTWADKDAYDAKAKELA
                     QKFNHNFEKYASEASAEILAAAPKA"
     gene            7967..8428
                     /locus_tag="PLF48_01995"
     CDS             7967..8428
                     /locus_tag="PLF48_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48122.1"
                     /translation="MCKKLTIPGSLYGTYVQYLARRQYEQSNHQGYVMATFCDVKTYT
                     GGSYTAKVLSAQNYYPFGMQMPNKNYNLPSYSSLNKYRYGFNGMEKDDEVKGTGNQYT
                     TLHLGYDARLGRWFSIDPKFKLQPWQSPYNAIDNSQIWKNDPHLVQVCQKK"
     gene            9153..9332
                     /locus_tag="PLF48_02000"
     CDS             9153..9332
                     /locus_tag="PLF48_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48123.1"
                     /translation="MSIEQTIKTMGRQPDAIYEIGRYDSLHHGTIQLDYYFKNQNTQI
                     LFGSNLKIVEIQHLD"
     gene            complement(9344..10384)
                     /locus_tag="PLF48_02005"
     CDS             complement(9344..10384)
                     /locus_tag="PLF48_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNF48124.1"
                     /translation="MQSATWNNTRNFLSKLTLYRVWNMLLIYLSYYFSKAFKKNIRAG
                     LPFAVAFEPTTSCNLRCPECPSGLRSFTRPTGMLDTGFFKKQIDAISEHTLYLTFYFQ
                     GEPYLHRDFLEMVGYANAKKIYTSTSTNAHYLSKANAIKTIESGLDRLIISIDGTTQE
                     TYQQYRIGGQLEKVLEGARNIIQAKKELNAATPHVVFQFLAVKPNEHQIADLYKLADD
                     IGADEVLLKTAQVYDYENGNPLIPENEKYSRYKKTATGKYVLKNTLDNHCWKMWHSCV
                     ITWDGNVVPCCFDKDAQHVFGNLSQHTLTEIWNNQKYQQFRNRLIQSRKSIDICTNCS
                     EGCEVNFSLESY"
     gene            10644..11156
                     /locus_tag="PLF48_02010"
     CDS             10644..11156
                     /locus_tag="PLF48_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48125.1"
                     /translation="MTTQDLIQEISAHPKMVIILLGIPPLLAILFSFMFKKEDIPNSN
                     LKYLYSVLVYLSCIPGVISVILILYTFFFLKGNFLEVDVLAYFLPLTSMIVTLLLIKR
                     TIAFDLIPGFKRLSGVFVLIAVAFIFSLVLYNLNFHVGVFFLGSVKSLVFIFILLLAL
                     LVYGYNKVVK"
     gene            11404..12483
                     /locus_tag="PLF48_02015"
     CDS             11404..12483
                     /locus_tag="PLF48_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNF48126.1"
                     /translation="MGIFDKLRNEFIDIIEWTDNTQDTIVWKFPRYQNEIKYGAQLTV
                     RETQVALFLNEGKIADVFQPGRYELTTANMPIMTTLRGWKYGFNSPFKADVFYVNTKQ
                     FTDQKWGTKNPITINDNRFGMIEIRAFGNYSFRVVDAEKFFKEIVGTDGEFTTEEINN
                     QLRTLIVSKLTDAFAESNIKIEQFAANLDELSKFGAEKLSDDFDQYGLKVTSLLVENI
                     SMPDDIKKEIFELSRLDKIDMQKLTQYKTAKGIEKAAESGGMAGAFVGMGMGNIMGST
                     MQNAQQNSNTPPPVMQIFVAQNGQQTGPFDIPQLQQMAQGGQLTKETLVWKAGMAAWA
                     KAETLAELGTILNSVPPPIGPPPIA"
     gene            12555..13283
                     /locus_tag="PLF48_02020"
     CDS             12555..13283
                     /locus_tag="PLF48_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF48127.1"
                     /translation="MKYLLIALCLLSSSFVFSGNTELKKDSTELEIYDRSEVMPSFPG
                     GQYALTNFLSKNIRYPIIAHENALEGKVIVKFYVDTDGSIKNPIVLKDNVGGGCAEEA
                     IRVIGLMPKWNPGTQRNKPVRVFFVIPITFKLTNAESTTTPIISKAYPLCGFDSLQYY
                     LSNATKVIAKPKKDRKKQYHVQATFTVLADGNISNVVISEITKDDDKIKSALIEYIKR
                     GPKWLPEKVDSIARPSFQKVEFSF"
     gene            complement(13394..14128)
                     /gene="rlmB"
                     /locus_tag="PLF48_02025"
     CDS             complement(13394..14128)
                     /gene="rlmB"
                     /locus_tag="PLF48_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNF48128.1"
                     /translation="MKQQLIAGRNPVWELVQSNTEVEKVLISKSAHGDIIKQIIETCR
                     EKNIYVQFVPEVKIQSLSKANHQGILAFTSLIQYFDVQEIIDFIFDKGENPLLLILDG
                     ITDIRNVGAIARTALGLGVHAIIIPKKGSASINDDAVKISSGALLKIPVCRVDQLSTT
                     VKDIKNNGITLIGLDGQGKKFIADTELNKPLALIAGAEDEGISKDVQKLLDEKVKLPM
                     SEELESYNVSVATAIALYEVYSGRMK"
     gene            complement(14221..14469)
                     /gene="atpC"
                     /locus_tag="PLF48_02030"
     CDS             complement(14221..14469)
                     /gene="atpC"
                     /locus_tag="PLF48_02030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNF48129.1"
                     /translation="MTLDILTPEQSLFSGTADRVQLPGINGKFEVLDNHAPMIATIKE
                     GKIKVTTGNNKQEIKVKSGIVEILKNKVSVLVEVSAEE"
     gene            complement(14540..16045)
                     /gene="atpD"
                     /locus_tag="PLF48_02035"
     CDS             complement(14540..16045)
                     /gene="atpD"
                     /locus_tag="PLF48_02035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNF48130.1"
                     /translation="MANIGRITQIIGPVVDVTFAGENSKLPEILNALTVKKDDGTEVV
                     LEVQQHLGEDTVRTVAMDASEGLTRGAEVTDTGDQIKMPAGEAIKGRLFNVIGNAVDG
                     IGEVSKVGGYSIHRKPPKYEDLATTKEVLLTGIKVIDLIEPYAKGGKIGLFGGAGVGK
                     TVLIMELVNNIAKGHGGLSVFAGVGERTREGNDLLREFLESDVIKYGEAFKHSMEKGD
                     WDLSAVDKNALLESKATLVFGQMNEPPGARARVALSGLTIAEQFRDGDGEGKGKDILF
                     FVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKNGSITSVQAVY
                     VPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILDPQVLGERHYN
                     TAQRVKMILQRYKELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFHVAEQFTGL
                     KGVFVPIEETLRGFEMIMDGEVDEYPEAAFNLVGTIDDAIEKGKKLLAEAK"
     gene            16423..>16881
                     /locus_tag="PLF48_02040"
     CDS             16423..>16881
                     /locus_tag="PLF48_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48131.1"
                     /translation="MIRVFFAVIFSVVSYALAQQMPEKCATHTHQLTLLQQHPEISNE
                     KERINTLIQILKANQSANKNEQVIITIPVVVHVVYNTTVQNISDAQIQSQISALNKDF
                     RKLNSDLLPSNHPFFSLIADVQIEFCLASIDPSGNATTGITRTNTSVSSFS"
BASE COUNT         5168 a         3210 c         3084 g         5419 t
ORIGIN      
        1 gtcaaaggct gctttaatct gcacaacttt tcccgatatg aatgggcagt tttatcctgc
       61 cgccattaac tttgccaata ccgacgtgta tgaatattcg accaaattat tatttcagtt
      121 tctgcaccgc accaatgcct acctgatttt tatttgggta attttccttt tctttataac
      181 aaaaaaatgg aacgatatct ttgagtttca gcgcacccgt tggttgctac ttattatggt
      241 gggcatacaa ctattgcttg gtattttcac attgctaaat tcgcaaggca gtataccggt
      301 tgtgctgggc gtattgcatc agctgggagc cttcttgctg cttatttttt ctatcgaagg
      361 attttatttt atttcgtata aagggaaaga acaaaaagta gtttaatcac tctacttact
      421 ttaacttgaa cacagcaaac aggatatgaa aattaaaatt gcacatatca gcaacttgtc
      481 agatgctcgt tttttcagtg catttggtgt agattatttt ggcttcagtt tggatagttt
      541 aaatcctaat gctgttactg taaatgaggt gaaagatatt actcagtggt tgtataaacc
      601 catactggtt ggcgaattcg gcatacatca aaatcaagct gaaattgctt acttagtaca
      661 acaactttct ctgcaagaaa tacaaattcc gtttgcgcat ccggataaag atttgatgga
      721 tttttccaaa tttatacaaa ttgattataa cgaaattcaa aaggtaaccg gatcagcaga
      781 ttattttatt ttatcgctaa ataataaaca attagcagac aaggctgtat tggattttat
      841 tcagcagcac aaagtatttt tggaagctcc tgttgaacca gaaatgctga atgcatggtt
      901 aactattgtt cgtccttatg gtattcaaat tcgctgtcaa agagagagcg aagattgttt
      961 gtttaacaca gactattacg ctgaggtttt ggaaattatt gagcaacaca ccacttaaaa
     1021 aacgtatttt tatgcgtatg tcaactccaa accgaggcag tatttttttg ttaaccatag
     1081 tgctttttgt ctttttttgg atatataatt tcccgttttt ttggaaactg cctccgcaag
     1141 gctcgcatat ttggcggcag tcggattgta tggcgatggt caataatttc aaagaaaaca
     1201 agctgccatt ttttcagccc gaaacgtata atctgcaatc ggagcaaggg aaatgcgccg
     1261 gtgagtttcc gatattatat tatatcgccg caaaattttc cgacccaact tatgccctgc
     1321 gtgtgataga tagtttcatt tttattctgg gtgttattgc ggtatttttt attgcatttt
     1381 attttttaca ggatgaattg cttgctttgc ttccctcctt actcttgttt acttcgccac
     1441 tgttggtttt ttatggcaat aattttttgt ctgatgttcc ggcattgagt tttgcttata
     1501 tcgggtgggc tttttttttc tacagcgaag gcaggaagtc taaagtaatt tttctactgg
     1561 ctctggtatt ttttgttttt tctgttttgt tgaaagcaag ccagttgctc aactttgcca
     1621 tcttgtttat ctatttattt gcttacaaaa aagaagctaa attcagcagt ctttttagtt
     1681 tgtgcttgtt tgcactgatt cctgttgcat ggtatgctta tgcaaaatgg tataatgcac
     1741 agcaccacga cacgtattat tttttaggta tttatcccat ttggaaactc agtgtgtatg
     1801 agattggatt atgtatctgg cgcatgacga tgtcttgggg taagaattat ttttggccta
     1861 caacttctgt tttattgata atagcaagtg cattgttatg gaagcataaa aataaattac
     1921 atccggaatt acggctattg ttgctctatt catttttgtt taccacgcta tatatattac
     1981 tttattttca acgcatgatc agacatgaat attactatgt tgctttttat gtgtttgttt
     2041 tatttttctt aatctccttg ctcctgcttt ataaaaaatt ttatgccgaa aatatttttt
     2101 cgcatacagc tttatttctt tttctaattc ccaatcttgt tttttgcaac tattatatac
     2161 atgaaaaact gaaagacaat cgtccaaatg ccgttctatc gtcatcggga tttcaatatt
     2221 ttctcgaaaa aaatggggtt acgaaagata aaatggtttt gatattgccc gacatctcta
     2281 ccaatattac actcaatcaa attcaccgga agggttacac tgcgtataat actatagatg
     2341 aagttttgaa acagcacaag gcagatttta ttcttttagg tgatagctcc ttaattacga
     2401 acatgcgtaa atatatagca acagatacag ctgccacata cagagaaatg tttttataca
     2461 ggctaaaaaa ggaataatcc ggcttaccag atgcggttat ccatttttta cacgtcatct
     2521 cgaacgtagt gagagctccc agtttgtatg cagatgtcca atgcaaagag attcctcact
     2581 actttcggaa tgacgtgtgc caagttgtat gggattcctc tctgtctgtc cgcttcgcag
     2641 caggcggacg ctcggaatga cgtgctgctt caattacata agaactaata tcgtccgaca
     2701 aatccaactc tctcccattt tcgtttataa ttacataatt ctactttcag tgttgttctt
     2761 tttatcttta tgccaaatat cagcgtatgt tgacgataga acaactgaaa caggataccg
     2821 cctttgcaaa agaaagaatt gcacacaaaa atttcaagga tattcatctg gtagatgaaa
     2881 tattggaaat ggaagaagaa cgcaaaaaat taaaaacaag tttagataat tatttgtcgc
     2941 aacgcaacac gatttcaaaa gaaattggtg cgttaatggg caaatcaaaa ggactgagcg
     3001 gtgatgcatt agctgaagca aaagcgcagg aagagaagtt gaaagctcaa attgccgatt
     3061 tgaaaataca aatagatgaa gccgaatctc aacatgataa aatagttgca gcgcagtttg
     3121 aaaagctggt tttgttgccc aattttccga atgctattgt tccaaaagga aacagcgctg
     3181 aagataatga aatcacattt tctgttagtg agttcccgtc attgcccgaa agtgctttgc
     3241 cccattggga actggctaag aaatatgatt taatcgactt tgaattaggc aataaaatca
     3301 caggagcagg gtttccggtt tataaaggaa aatgtgcgca attgcaacgt gcgttgattg
     3361 catttttctt agataaagca actgaagccg gctatgcaga agtgatggtg ccgcatgtag
     3421 tcaaccgcga ttccggattt ggtacgggac aacttccgga taaagaaggg caaatgtatc
     3481 atatcggtat agatgatttg tatttgattc ctactgctga agtacccatt accaatctct
     3541 accgggatgt cattatagat gaaaaaaatc tgcccataaa aaatgtgggt tatacccctt
     3601 gttttcgcag agaagccgga agctacggta gcgatgtgcg cggcctaaat cgtctgcatc
     3661 agtttgataa ggtggaaatt gtgcgtattg aaaagccgga aaattcttat aaagctttag
     3721 aagaaatgtt gcaacacgta gaggggctgc ttaaatcgct taaattgcct tatcgcattt
     3781 tgagattatc aggaggcgat atgacttttt cgtctgctct gacttatgat tttgaagtgt
     3841 attcagccgg acaaaaaaaa tggctcgaag tgagctctgt ttctaatttt gaaacctttc
     3901 aggctaatcg tctgaagctg cgcttaaaag acaatgacaa taaaaagcgt ttagctcata
     3961 ctttaaatgg gagcgcgtta gccttgcctc gtattatggc ttccatttta gaaaattatc
     4021 aaacagcaga tggcattaca ataccggagg tattagtacc ttatactaag tttgaaaaaa
     4081 tttagttgcc ctttatttgt gtaattcaat aaatttgagt gcatctatct gaaattcctg
     4141 atattgctca gcgtgtggta aatgtgctga aacatcatac cgataaggtt caaatttaat
     4201 atccgatcca ccgtaattat ccgcatactt ttgggctaaa ttatcaaatg gcaacagacc
     4261 atcctttcga ccccaaatca acaataatgg aactttgatt ttatagagct gatcggttaa
     4321 gtccatttca aaaagtgaag cctgattata agacaagtga ttgatggtaa acatgctatt
     4381 gtacgaaccc acaaagcttt catattttgg caaagtaatc gcctgggcag cgagatttga
     4441 tgtattataa actatgccgt ttacttgtcc cagaaatgac aaatgttgct gaaacatgtg
     4501 gacattagtt atggcaggat aggctgcgta aatatctttt acattttgcc agtaggcatc
     4561 atttacgtgc aaagcaagca tagagtctgc tcgttgtaaa agccagttta ccgataactg
     4621 catgatgcca tgttttaaat cataggcagg tgcttctact attgcaccct taactttctt
     4681 ttgatggagt gtatctaata aataataagt agttaagcct ccgccccaac tgattccatg
     4741 aataaatatt ttatcagcct ttactacttg cttggtaaaa tctacgatat tatctaaatc
     4801 ttctgaaaat tgttcaatgg taattgtgcc ggggtccgta tttccttggg tcaatccgga
     4861 acctcgttga tcccaataga ctactttata gtacttctct atttctttcc aataagcata
     4921 agcccgttcg ccttgagcac ttccgccggg accaccatga ttaaacacaa tggcaatatc
     4981 cgaattaatg ttaccacaca ctcgaactgg caatgcaaca tctccaactt ttacgaaata
     5041 gtaattatcc agttgccctg aagctacctt agcctccttt ttgcatgata ttaagagcag
     5101 gataaaacct atgatatatg tatgcaacct cattttcatt ctgatttttt tctgattaca
     5161 taacttattc ccacttcaaa tataaggcgt cgcacccatc tgccattatt gggataacgg
     5221 tataataaat tgcatcccaa atgtatatcc aatggtgcat ccgtcttttt agaaaaatca
     5281 taaccaaaat ctactttgcc acccaaaagc aaattgtgat aaccaaaaga tacttttttg
     5341 aatgtgccat ctgcctgacg ttcataaaca ggagcattta atttggtgta catatatccg
     5401 gcatcaaagc tgacctctga aaaaatacct acattaaacg tatgccgata agttaattcc
     5461 ggaataaaaa agatgcctgt attatatcgt ttgcgataga tatacccaaa tgcaggtcgt
     5521 aaaaacaatt cgcgttctat tattttgggg ttatactcgc tcgtgccttt attcaactta
     5581 ggtttgtaaa agaagcgata agcccctgaa atggaaagtc catagctgat ttgcatcggc
     5641 tttacaaatt ctacatctgc aagtgcgttt actgctatta tgtttttttt gaattttaat
     5701 ccgtctattt taattatttc cttatagtcg gtttgctctt gtgcaattgc cgcataagta
     5761 caaaggatgc taaagcaaca taaaaataaa tagcgtacaa aagagtgcat aatattcagc
     5821 acaaattaaa acaaataatc gaaaaaattt aattcttacg ctttcggagc cgcagctaaa
     5881 atttctgcgc ttgcttcaga tgcatatttt tcaaaattat gattgaattt ttgtgccagt
     5941 tctttggctt ttgcatcgta cgcatctttg tcagcccaag tgtttctcgg gttcaaaatt
     6001 tctgccggca cacccgggca agttaccgga atttgcaaat caaagatagg cagtgtttca
     6061 tagctcaccg catccaactc tccgcttagt gcagcagtta tcagcgctct ggtatatttc
     6121 aggctcattc gttttccaac accataagca cctcctgtca taccggtatt aatcaaccat
     6181 acattagcat gactttgtgc caatttatta cccagcatag ccgcatattt tgcaggatgc
     6241 aaaggcagaa atgccgctcc aaaacaagct gaaaacgtgg tttgcggttc tactacacct
     6301 tcttctgttc cggctacttt agatgtgtaa cctgaaataa acaggtacat agcttgtgcc
     6361 gatgttaatt tgctgattgg aggcaatacc ccaaaagcat cataactcag gaagaaaata
     6421 tttttaggca caccccccac tgaaggtact gcaatattat gtatatgata gatagggtaa
     6481 gaaactcggg tattttccgt ttgggaaata ttggcataat cgacagtatt tgttcctgga
     6541 taaaactcaa tattttctaa taaggctccg tgattaacag cacggaaaat atccggctct
     6601 ttttcttctg tcagatccac acatttggca taacatccgc cttcaaaatt aaatacgctc
     6661 gtatcactcc agccatgctc atcatcgcca atcaatttac ggtttgggtc ggcggagaga
     6721 gtggtttttc ctgtgcccga caagccaaaa aacaaggctg tgtctccacc atcgccgata
     6781 ttggcagaac agtgcatgct cagcacattt ttgagatgcg gtaattcgaa attcaatacg
     6841 gtaaaaatac cttttttgat ttctccggtg taggcgctac cacctatcaa tactattttc
     6901 ttggtaaaat taatgataga gaaatttccc tgacgagtcc cgtcaacggt tgcatcggct
     6961 aaaaatccgg gagcattcag gattagccat tccggctctg ccgtttggat ttgtgcatca
     7021 tccaaacgca ggaacatatt gtcggcaaat aaattagccc aaggtgtttc tgtaactaca
     7081 cgaatgccga gggtatactc cggagcggca caagctttta cgtctcttac aaaaatttgc
     7141 tgtaaactat ccagataagc gactaccttt tcttgtagcg catcgaattt ttccggtgaa
     7201 aatggctgat tgattttgtt ccagtccaca gcattttcgg tcagttcatc ttttacagta
     7261 aatttatcag cgggagagcg ccccttgaat tttccggtgt ttacacagag agctcctgta
     7321 tcattcagca cgccgagatc gaggtcgagg gttttttgta tcagattttc acgagataag
     7381 ttccaaagtg cggtggtttt agaaaagccc aatttgctaa ggtcagcaga agggttcttg
     7441 acgccttgtt cattcattgt tgataatttt gcgtgtagca aatgtaagaa aattgcctct
     7501 aaaatccttt cgttaatatt ttagtaacac aaatccgata aatgagaaaa aacattattg
     7561 aatattgatt ggttagtaaa gattgactgt gcaaatagtt tcgaagcagc ataagtcaaa
     7621 gatagatgtg tcacgatgtt cgtggcacat ttatatcgaa caataaattt gtttaaaagc
     7681 aatgaattga gaaattaatt tacgaataac cggcaaaaaa tgcagtttaa aaagataatg
     7741 cagtttttga aactgaaaaa ataaaaaagc acttagcgcc attcccaaat acttaatttt
     7801 ctcagtaatt gagacaagaa tcgagcgtgc gcccgacccc caattagcac aagtatgata
     7861 aaattaaaaa aataaaaact atcccgaatt atcggaaaaa gtatgcggcg taggtataag
     7921 cggggacgta cttgcctgcc tgtcggcaga ccgggactgt tatattttgt gtaaaaagtt
     7981 aacaatacct ggctcacttt atggcaccta tgtgcaatat ttggcacgcc gccagtatga
     8041 gcagagcaac caccagggct acgtgatggc taccttttgc gatgtaaaaa catataccgg
     8101 aggcagctat acagccaaag tactgagtgc acaaaattat tacccgtttg gtatgcaaat
     8161 gccgaataaa aattataact tgccatctta tagttctttg aataaatacc gctatggctt
     8221 taacggtatg gaaaaagatg acgaggtgaa aggaactgga aatcaataca caacacttca
     8281 tctaggatat gatgctaggt taggcaggtg gtttagtatt gaccctaagt ttaaattgca
     8341 accttggcaa agcccatata atgcaataga taatagtcaa atttggaaga atgatcccca
     8401 cctcgtccaa gtatgtcaga aaaaataaaa actggcgcaa gaatgaggcg caggagtagg
     8461 cgatagcttc ttgtgccgtg ctagctcact acgttcgcct ttgttggttt gtatccaaca
     8521 aacttgaaac ccttgctaat gtaaatgtta acaaactaaa agccactatt aaaccactca
     8581 caatcccttt tacaggctat gaaagtgaaa tgcaagataa gcctgaacaa ggtataaaat
     8641 cttttgatcc agattgttac tagtataaaa tatctataat atatgattta taatacactt
     8701 tccttactaa ttattatttc atacatatct actgggatag gatggctatt aagtatgcgt
     8761 cagtatttta gaataaaatc atataaaaat agtgtaaaaa atatgaatat aatacttgaa
     8821 catactataa atggtctaaa gtatatgttc gattatgatt ttatagaata ttttccgatt
     8881 actatttttc tcaaacaatt cgacagttct gcatataata attatagtga tgagttaaag
     8941 aagctatatt cggataaaca taaatatgaa atagtattta tcatgtcaat tattatgata
     9001 tttgtgttta catttattat tacatgaggc aaaataataa gaaccaaaca atagcatgca
     9061 ttaaatttat atattatata attatattac taaatttaat tagttgtaac aacaacactt
     9121 tgccagacag gtcttatctg ttaaaatctg gaatgagcat agagcaaacc ataaaaacaa
     9181 tggggcggca acccgatgct atctatgaga ttggaagata cgactcttta caccatggga
     9241 caattcagct tgactattat tttaaaaatc aaaacacaca gatactattt ggctctaatt
     9301 tgaaaatagt ggagatacaa catctcgatt aatttctatg ttttcaatat gattcaaggc
     9361 tgaaattcac ctcacatcct tctgagcaat tggtacagat atcaatactc ttacggcttt
     9421 gtatcaatcg gtttctgaat tgctgatact tttgattgtt ccagatttct gttaatgtat
     9481 gttgcgagag attgccgaac acatgttgcg catctttgtc aaagcaacaa ggtacaacat
     9541 tgccatccca ggtgatcaca caactgtgcc acatcttcca gcaatggtta tccagcgtat
     9601 ttttcaatac atattttcct gttgccgtct ttttatatct cgaatacttt tcattttcgg
     9661 gtatcagcgg attaccgttt tcataatcat atacctgcgc cgttttcaac aatacttcat
     9721 ccgctccaat atcatccgct aatttgtaca aatccgcaat ttggtgttcg ttgggtttta
     9781 cggctaaaaa ctgaaaaacg acatgtggtg ttgcagcatt taattccttt ttagcttgta
     9841 taatattgcg tgcgccttcc agtactttct ccagttgtcc tccaatgcga tattgctgat
     9901 acgtttcctg tgtagtgccg tctattgaaa taatcagacg gtctaaacca ctttctatcg
     9961 tttttattgc attggctttg cttaaataat gcgcattggt tgaagtggaa gtatagattt
    10021 tcttagcatt cgcatagcca accatctcta aaaaatctct gtgcaaataa ggctcaccct
    10081 gaaagtaaaa cgtgagataa agggtatgtt cggagatggc atctatttgt tttttaaaga
    10141 agcccgtatc cagcattccc gttgggcgtg taaacgaacg caagccactg gggcattccg
    10201 ggcaacgcaa attgcaggat gtagtgggtt caaatgctac ggcaaaaggc aaacccgctc
    10261 gtatgttttt tttaaaagct ttggaaaaat aataggataa ataaatcaac agcatattcc
    10321 aaactctgta taaagtaagt ttagataaaa aattacgggt attattccat gtagcagatt
    10381 gcatgattcc gccgacaaat tagtacattt ttacaacata tcagaccaga cttcgggttt
    10441 aaaaaataat gagataaaat agatgatttc tcattttttc ctccaactta acaaggagga
    10501 ggaattaaaa ggtggtggtt cttaaaaaaa atcaactatt ttttttaatt acttttaccg
    10561 aactcaggca atcaggcatg atatataaag caggaagtag gctatttatg aaaatatccc
    10621 tacttttatt tttttatatc cctatgacta ctcaagattt aattcaggaa atatcggcgc
    10681 acccgaaaat ggtaatcatt ttgttaggca tccctccact tctggctatt ttattttcat
    10741 tcatgtttaa aaaagaggat atcccaaatt ctaatctgaa atacctctac tcggttttag
    10801 tttatttaag ttgtattccg ggtgtaattt cggttattct tatcctctat acttttttct
    10861 ttttaaaagg aaattttctg gaagtggatg tattagctta ttttctgcca cttacatcca
    10921 tgattgttac gctgttgctg ataaaacgaa ccatagcttt tgatttaatt ccgggcttca
    10981 aacgattatc gggagtgttt gtcttaattg cagttgcttt tattttttct ttggttttgt
    11041 ataatctgaa tttccatgtg ggtgtcttct ttttaggaag cgtaaaatca ttggttttca
    11101 tttttattct tttattggca ctgctggtgt atggatataa taaggtagtt aaatagttta
    11161 atggttaagt agtttaatag tttgttgatt taataagctc ttgtgtgtta gatttatccg
    11221 tgtgcagtca ggtctttcga ccttaattaa gtatttattt ctttttcaca tagaatggaa
    11281 acatacgaca acacagaaac atctgacggt atgcaaaata aactgtggaa cttttatttt
    11341 cagttttttg ttataacaga actattttaa tgcttatttt tatacaattc aaaaatcaag
    11401 attatgggca tatttgataa actgcgcaat gagttcattg atattatcga atggacagac
    11461 aatacacaag acactatcgt ttggaaattt ccgcgatatc aaaatgagat aaaatacggg
    11521 gcgcaactta ccgttcgcga aacacaagtt gcactttttc tgaatgaagg taaaatagcc
    11581 gatgtttttc agcctggcag atatgaactt accaccgcca atatgccgat aatgactacg
    11641 ctacgaggat ggaaatatgg tttcaattct cctttcaagg cggatgtttt ttatgtaaac
    11701 accaaacaat ttactgacca aaaatggggt acaaaaaacc cgattacgat aaatgacaat
    11761 cgcttcggta tgatagaaat ccgtgcgttt ggcaattatt ctttccgtgt ggtagatgcc
    11821 gaaaaattct ttaaagaaat tgtgggaacg gacggagaat ttactaccga agaaatcaat
    11881 aatcagctgc gcactttaat tgtatcaaaa ctgacagatg cttttgcaga aagtaatatc
    11941 aagattgagc agtttgccgc taaccttgac gaactttcaa aattcggtgc ggaaaaactt
    12001 tccgacgatt tcgaccagta cggcttaaaa gtaacatcgc ttttagttga aaatatctct
    12061 atgccggatg atatcaaaaa agagattttt gaattatccc gactggataa gatagacatg
    12121 caaaaactga cacaatacaa aaccgcaaaa ggcattgaaa aagctgccga aagcggtggc
    12181 atggctggtg catttgtggg catgggcatg ggcaatatta tgggtagcac tatgcaaaat
    12241 gcccaacaaa acagcaatac tccgcctcct gtgatgcaaa tatttgtagc acaaaatggg
    12301 cagcaaacgg gaccattcga tataccgcaa ttacagcaaa tggcacaagg cggtcaactc
    12361 accaaagaaa cactggtttg gaaagccggg atggcagcat gggcaaaagc agaaacatta
    12421 gcagaactgg gcactatact caattccgtt ccgccaccga taggcccacc gccgattgct
    12481 taaaacagct gttacttttc ctggatttta tcttgtaata gtaatatttg attatatttg
    12541 ttacaaaaat aaagatgaaa tatttattga tagcactctg cttgcttagt tcttcgtttg
    12601 tattctccgg caatacagaa ttaaagaaag atagtactga gttggagatt tatgacagat
    12661 cagaggtgat gccctccttt ccgggtggtc aatatgcatt aactaacttc ttgagtaaaa
    12721 acattagata cccaataatt gctcatgaaa atgcattgga agggaaggta attgtcaaat
    12781 tctatgtgga tacggatggt tctattaaaa atccaatagt actaaaagat aatgttggtg
    12841 gtggttgtgc agaagaagca attagagtga ttggattaat gcccaaatgg aatcccggca
    12901 ctcagagaaa caaacctgta agagtatttt tcgtaattcc gatcaccttt aaattaacaa
    12961 acgctgaatc gactactacc ccaataatat caaaggcata ccctctttgt ggttttgatt
    13021 cacttcaata ttatctatct aatgccacta aagtaatagc aaaaccaaag aaagacagga
    13081 agaagcaata tcatgtgcaa gcaacattta ctgtattagc agatgggaac ataagtaatg
    13141 ttgtaatttc cgaaatcact aaggatgatg ataaaataaa aagtgcatta atagaatata
    13201 taaaaagagg accaaaatgg ttgcctgaaa aagtagattc cattgcaaga ccttcctttc
    13261 agaaagtgga gttttcattt taataaacag agtaaaccct ttctctgggt attaaatatt
    13321 tattttgcga ttttgaataa gaaacgaaag gactgtttat tagtttagtg tataaatcta
    13381 attcaaacaa ttactatttc attctcccac tgtagacttc atataaggct atagcagtgg
    13441 ctacggatac gttataggat tctaattctt cagacatggg tagttttact ttttcgtcca
    13501 gcagtttttg tacatctttg gagatgcctt cgtcctctgc acctgcaatt aacgcaagtg
    13561 gtttatttaa ttctgtgtct gcaataaatt tcttaccctg accatctaag cctattaaag
    13621 taatgccatt attttttatg tcttttacgg tagtactcaa ctgatccacc cgacaaaccg
    13681 gaattttcag caatgctccg ctggatatct taacagcatc atcattgata ctcgcacttc
    13741 cttttttggg gatgatgatg gcatgcacac ccaatcccaa agccgtgcgt gcaattgcgc
    13801 ccacatttcg gatatcggta atgccgtcta aaataagcaa taacggattt tctcctttgt
    13861 caaaaataaa gtcaataatt tcctgaacat cgaaatactg aattaaagaa gtaaaagcca
    13921 gtatgccctg gtgattcgct tttgataaag actgaatttt aacttctggt acgaattgta
    13981 cataaatatt tttttcgcgg caagtttcta taatttgttt gatgatatca ccatgtgcac
    14041 tttttgaaat cagcactttc tctacttccg tattcgattg taccaattcc caaacggggt
    14101 ttctgcctgc gataagttgc tgtttcattt tgctgtaaga taacaagaat tgcgcttgct
    14161 gaaaataaaa aacgggaaac ttattttgtt tcccgttgat attgtaaaat tagttaaatc
    14221 ttattcttca gctgatactt caactaacac agaaacttta tttttcaaaa tttccacgat
    14281 accgcttttt acttttattt cttgcttatt atttccggtc gttactttta ttttgccttc
    14341 ttttatggtt gcaatcattg gtgcatggtt gtccaacact tcaaatttgc cattgatgcc
    14401 cggcaattgt actctgtccg ctgttccgga aaataaactt tgctctggtg taaggatatc
    14461 taatgtcatt ttaatttgag tttaaagttg aatgctcaat gaataatatt catcattcag
    14521 catttaccgt ttaccatttt tatttcgctt ccgctaataa ttttttacct ttttcgatgg
    14581 catcatctat agtacctacg aggttgaatg ctgcttccgg atattcatcc acttcaccgt
    14641 ccataatcat ttcgaagcct ctgagtgttt cttctattgg aacgaatact ccttttaagc
    14701 ccgtaaattg ctctgccacg tggaaaggct gagataagaa acgctgaaca cgacgtgcac
    14761 ggtgtaccac caatttatct tcttcgctca actcatccat acccaagata gcaataatgt
    14821 cttgtaactc tttgtagcgt tgcaaaatca ttttcacgcg ttgtgcagta ttataatgtc
    14881 tttcacccaa tacttgtggg tcaagaatac gagatgttga atctaatggg tcaaccgcag
    14941 gataaatacc caactcggca attttacggc ttaataccgt agttgcatcc aagtgggcaa
    15001 atgtagtagc cggagccggg tctgttaagt cgtcagccgg tacataaact gcttgcacag
    15061 atgtaataga accattttta gtggaggtaa tacgctcttg catcaaaccc atttctgtag
    15121 ccaaggtagg ttggtaacct accgcagatg gcatacgtcc caataatgca gacacttcag
    15181 aacctgcttg tgtaaatcgg aagatattgt ctacgaaaaa taagatgtct ttaccttttc
    15241 cctcaccatc accatcgcgg aattgctctg caatggtcaa tcctgataat gcaacacgag
    15301 cacgtgctcc cggtggctca ttcatctgtc cgaaaaccaa ggtagcttta gactccagca
    15361 atgcattttt gtcaacagct gataaatccc agtcgccttt ttccatagaa tgtttgaagg
    15421 cttcaccata tttaattacg tcagattcca agaactcacg caataagtca ttcccttcac
    15481 gcgtacgctc acccacaccg gcaaatacag ataaaccacc atgacctttg gcaatgttat
    15541 ttaccaactc catgattaat acggttttac ctacacctgc accaccaaac aaaccgattt
    15601 taccaccttt tgcatagggc tcaatcaagt cgattacttt gatacctgtt aataatactt
    15661 ctttggtcgt ggccaaatct tcatatttag gtggtttacg atggatagaa tatccaccta
    15721 ctttgctcac ttcaccaata ccgtcaacgg cattgccgat gacattaaac aaacgacctt
    15781 tgatagcctc accagcaggc attttaatct gatccccggt atctgttact tcagctccgc
    15841 gagtcagacc ctcggaagca tccatggcta ccgtacgcac tgtgtcttcg ccaagatgtt
    15901 gttgtacttc caaaacgact tccgtaccgt catctttttt aacagttaaa gcgtttaata
    15961 tttcaggaag tttggagttt tctccggcaa aagtaacgtc tacaaccgga ccgatgattt
    16021 gggtaattct acctatgttt gccataaatt tgaaaggtta tcttttttaa atctgcgcaa
    16081 aattacgatt aaacgctttt ttatcaaaat attacgaaaa aagaagtcta taaataaaga
    16141 tttggtaatt tgaccggaaa tttatgctaa acattattgc caaattggca tttttaactc
    16201 gggtttggga taagtgtttc aatttaagaa tagttgattt ttcacatgga agctcctcct
    16261 tttaattcct cctccttgtt acattggagg aaaaaatgtg caatcaactt ttttcaccat
    16321 atttttctaa acccgaacta tgcttaatat gtgtttgtga taattttttg actgaaaaat
    16381 cagattaaat caaaatctat ttcatacatt tattcctaca ttatgatccg ggtctttttt
    16441 gcagtaattt ttagtgttgt atcgtacgct ttggcacagc aaatgcccga aaaatgtgct
    16501 actcatactc atcaattgac tttattgcaa cagcatccag aaatcagtaa tgaaaaggag
    16561 cgcattaata cattaataca aatattaaaa gccaaccagt ctgcaaacaa aaacgaacag
    16621 gtaatcatta ccataccggt tgtggtacat gtggtgtata atactactgt acaaaatatc
    16681 agcgatgcgc aaattcagtc acaaataagt gcactaaata aagatttcag aaagctgaat
    16741 tccgatttac tacccagcaa ccatccattt ttctcactga ttgcagatgt gcaaattgaa
    16801 ttttgtctgg cgagtattga cccatccggg aatgctacga ccggtattac ccgaacaaat
    16861 acttcagtaa gcagtttttc a
//
LOCUS       DAOQII010000021        16767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1638_length_16767_cov_5.354655, whole genome shotgun sequence.
ACCESSION   DAOQII010000021 DAOQII010000000
VERSION     DAOQII010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16767
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1638_length_16767_cov_5.354655"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLF48_02045"
     CDS             complement(<1..968)
                     /locus_tag="PLF48_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNF48132.1"
                     /translation="MKIAVNTRFLIKDALAGIGTYTQELFSRVVKLMPEHQFYFYFDR
                     KFDDAFIYSENIVPKMVFPPARHALLWYAWFEYAIPYRLKQDKIDLFVSPDGYCSLST
                     NVSQLLTIHDLAFEHYPQFIPKIVADYYKKYTPQFCKKADKIIAVSEYTKNDIINQYS
                     IFSDKIEVIYNGVDFKEINTGSKPGIRIDFPYFIFIGTFHPRKNILRLLKAFNAYKHS
                     KSNQMKLLLIGSDGWMNEDLLDYLENMVARQEVIWIKNCQREEIYQYLSCAAAMIYPS
                     LFEGFGIPIIEAMHCGIPVACSNTSCLPEIAGDAAIYFNPENEDEI"
     gene            1086..2201
                     /gene="dnaN"
                     /locus_tag="PLF48_02050"
     CDS             1086..2201
                     /gene="dnaN"
                     /locus_tag="PLF48_02050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNF48133.1"
                     /translation="MRFVVSAGQLLKNLQKISGVISTNTVLPILEDFLFDINSNSLTV
                     TATDLDTTMSVSMEVDSKEKFKVAIPAKILLDSLKALPEQPITITVDETTNAIELTTD
                     NGKYKLSGESSADFPKEPATEDVEEVNLAASILNKGVNKTLFAVSNDELRPAMTGVFI
                     QLNENGITFVATDAHKLVKFTRSDITGSDVSFIIPKKALNLLKSTVPNNDTIVKVQYN
                     KSNAFFTFDNISLICRLIDAKYPDYNAVIPKENPNLLSVNKDDFFSSLRRTSIFSNKT
                     THQVVLRMAGSELTVSAQDLDFSNEANEKLTCDYKGNEMEIGFNARFLLEMLSAVDSP
                     EINIELSTPNRAGIIKPTDLSENEDMLMLVMPVMLNS"
     gene            complement(2295..2492)
                     /locus_tag="PLF48_02055"
     CDS             complement(2295..2492)
                     /locus_tag="PLF48_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48134.1"
                     /translation="MGTKKLNKANDKKPKSKVKQNSILKEDNNLSLKEWERDIVVKCE
                     KTDEYISPDEIERTTFSREKH"
     gene            2583..2933
                     /locus_tag="PLF48_02060"
     CDS             2583..2933
                     /locus_tag="PLF48_02060"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNF48135.1"
                     /translation="MSVIFTSCQTDVKTLGKKFTESNPISVDELISKTKAEGHLDQAQ
                     VTGKIDKSCRSEGCWFTIKDNNGAEVYLDIKDKGFKMPLNSPERQVVALVNADTSVAA
                     ESGVSIDVKGVMFK"
     gene            3079..3151
                     /locus_tag="PLF48_02065"
     tRNA            3079..3151
                     /locus_tag="PLF48_02065"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3112..3114,aa:Lys,seq:ttt)
     gene            complement(3260..4183)
                     /locus_tag="PLF48_02070"
     CDS             complement(3260..4183)
                     /locus_tag="PLF48_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNF48136.1"
                     /translation="MKIQNFSAEPLHTLYNWIPNGLHADEIIFFPDACPGKSPLPTGT
                     VAITKQTDWRKFAVSDCGCGMLLAKSKYLHTDFDTKIWDKIYLDLKLQKGKLGDLGSG
                     NHFLDALRSDRDDYLYFLIHTGSRDESKLVDHLVDQPKKFDLTFESVCNWAADNRKAV
                     LKIVEKYFGRTEVILDKNHNHFELIDDFVVIRKGAAKLLPGEITVIPSNMVGDVVLVK
                     GKNKIQSVYYSICHGTGRTMSRAEAKLVSDNFDYNKLRTQIYIPEIIKNESIKTDAPF
                     CYRDLDTCLLLIADLVDVIQRFKPFAYLGQI"
     gene            4543..5277
                     /locus_tag="PLF48_02075"
     CDS             4543..5277
                     /locus_tag="PLF48_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNF48137.1"
                     /translation="MKRYRRIILLVFSLLLIAAVILFYIWNRKIYYPNFGIRIPSNYE
                     LHGIDVSRHQGWINWKQVATMKDKDIRLKFCFIKATEGVSHTDIFFKINWYRCKKNGI
                     KRGAYLYFHPAKNGKLQAKQFIKTVPLEKGDFAPVIDFEETNDLSKSKIIQSLKLCIS
                     TLETKYDKKPIIYCNVDFYNKFVKDELPDYPIWIAHYNRAFAPRINEQWSFWQHNCKG
                     HVNGIASDVDFNVFSNNDSELSKLCL"
     gene            complement(5287..6720)
                     /locus_tag="PLF48_02080"
     CDS             complement(5287..6720)
                     /locus_tag="PLF48_02080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF48138.1"
                     /translation="MNFNSRKQTIHQHFNDIAQSYRQYRKRFSYYGKDIINYFNFFLQ
                     DDCSVLEIGCGNGETIANLKGSYKAGIDFSENMIIAAQKNYTDVDFYVMDAENLLLDK
                     KFDVIIFNNIIGYLDNIQDVFYSIKKNCHQHTRIIISYYNHWWEPILNFGEWLGIKKK
                     SPQQNWLNEQDISNLLYLSGFETYRTNRRLLFPVYIPILSWLLNKYIAKLPLIKSLCL
                     NEFIFARILPHETEESVKEKYSVSICIPARNESGNIENAILRLPKFGKHQEIIFIEGN
                     SSDDTWEKIQEIQKKYASTHDIKIGQQDGKGKGDAVRKGYSMATQDILMILDADLTVP
                     PEELPKFYNAIATGKAEFINGSRLVYPMDKEAMRFLNTLGNKFFSKVFSWLLEQPIKD
                     SLCGTKVMSRQDYIELANNRAFFGDFDPFGDFDLLFGAYKLNLKIIDLPVRYRERTYG
                     DTNISRFTNGWMLLRMCWFAAGKIKFW"
     gene            complement(6723..7472)
                     /locus_tag="PLF48_02085"
     CDS             complement(6723..7472)
                     /locus_tag="PLF48_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF48139.1"
                     /translation="MKEHQPHLDLPETSLQHREIVLSKPFLKRIHSDWYNEFKNYSKS
                     IAIQGDVVEIGSGGGFLKDVYPEVKTSDIIKLPVCDMQFSATEMPFENQSLKGIFMLN
                     VLHHIPDNEAFFKEAERTLASGGFIYMIEPATTWFSKIIYKNFHHEPFDETVKEWKFA
                     SKGPLSDANGALPYIIFNRDAELFSKKFPELEIAKITFHTPVKYLLSGGLSKPALLPY
                     FMYGAVGFAEMILSPLASKLALFQTIIVRRK"
     gene            complement(7510..9291)
                     /locus_tag="PLF48_02090"
     CDS             complement(7510..9291)
                     /locus_tag="PLF48_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48140.1"
                     /translation="MAKQTPNKKNIEKQVVTKPVNAPSKKEVTKDGLNFNLFDFLENH
                     FAKNERKYLIALLLIAVVFSFFCFDTKISTANDDALYIEAGFDYSKNFFGYFYTANAP
                     LYPIVLSFLIRIFGVNLIALKMFSILFFALGIYFFFKAFRTRIPYIILIPALFIVVIN
                     FPFLMYASLTYAETFSLFIMGLTFYYLFKIFDKLKSESLTITDKIPMYLAIGGLVFLQ
                     MMTRNVALIFIGIVFLYFVYQKKIIDAIGSVLAFGTYYFIYKLVLKFIWKLDGSQFEA
                     QSRLMFQKDAYNASLGKENFSGFIDRFFTNAQIYISSRFMYVLGFREEMSPNNLILTL
                     VGVGLIAWAFILMWRKKQDLLMFTSLYFISFLAVTFLTLHTSWGQTRLIMIYLPLILF
                     SLFYILYYYGSINSMLQFILPLFVVVLFFSGISATSKKIANRLPIFIDNMTDNPYAGY
                     TSDWMNYIAMSKWCGENMPKEDYIACRKPPMSFVFSSGRKFYGIYNVPAQDADSLLTP
                     LKQQKVTHIFTSELRLDPDRYIENQYINTVQRYMYNIVQKYPNAFEFMHREGDIEKSD
                     LYKINWTYIDSLKSAMLTKPLQPVLTK"
     gene            complement(9272..10036)
                     /locus_tag="PLF48_02095"
     CDS             complement(9272..10036)
                     /locus_tag="PLF48_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNF48141.1"
                     /translation="MPSALSIKLGDFLYKHAFPVYNVIYPYFKRRQDKHDIALMQQYI
                     KQGDIVLDIGANIGFYTKILSALVGENGKVYAFEPDKTNFHHLMKNAGHLKNVVFFNK
                     AVNEKTGKITLYHSDLLNVDHKTYPTENYTSTTEIDGVAPDDVLPGHKVDFIKIDIQG
                     YEYYAFLGMKEIFKQNENLKIITEFYPYGMKNAKIDFEQFFNYLWENGFAVFMTLSDL
                     LVPFGKNDIFKHTQKMDEYTFIDLFIEKSKDGKTNS"
     gene            complement(10076..11026)
                     /locus_tag="PLF48_02100"
     CDS             complement(10076..11026)
                     /locus_tag="PLF48_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HNF48142.1"
                     /translation="MSYIPVLDLADYLSGDESRRQKFIQGFGKAYSEIGFAAIENHGI
                     DKDLIDKFYSQVETFFAMPTDTKLNYEKIEYAGQRGYTSFGREKAKQSEVADLKEFWQ
                     IGQYIEDGEIMPVDDYPDNIEVKELPEFNETGRLLYKNFEVSGRHLLRAIAEYLHLDV
                     NYFDAKIHNGNSILRAIHYPPITKEPESAIRAEAHEDINLITLLVGASADGLQVMDVE
                     GTWHDAKAEPHQIMVNVGDMLQRLCNNKLKSTTHRVVNPPKEKWHTHRYSIPFFLHPR
                     SEVRLDCLENCVDEQHPLAYAPISAGEYLDERLREIGLKK"
     gene            complement(11115..11693)
                     /locus_tag="PLF48_02105"
     CDS             complement(11115..11693)
                     /locus_tag="PLF48_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF48143.1"
                     /translation="MYPIVLSDNELVQNYINGDQQALAQLLNKYQRKVFSFILIKLKD
                     RTQAEDIFQETFFKVIDSLQQGRYNEEGKFLPWVLMIAHNLCMDYFRKNKRMPNTVDV
                     DSNVVQNSMGLLDENREDKIMRQQTADTLRDMIDKLPPDQKEVLILRHYADMSFKEIA
                     EMTGTNINTALGRMRYALLNLKKMIGNKIIVM"
     gene            11858..14638
                     /gene="uvrA"
                     /locus_tag="PLF48_02110"
     CDS             11858..14638
                     /gene="uvrA"
                     /locus_tag="PLF48_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNF48144.1"
                     /translation="MSNVKKLTKSTQKNILIKGARTHNLKNVDVEIPRDQLVVITGVS
                     GSGKSSLTIDTLYAEGQRRYVESLSSYARQFLNRMNKPDVDYIKGLSPAIAIEQKTTT
                     GTTRSTVGTLTEIYDYLRLLFAKVGKTYSPVSGKEVKKQDIADILAYITDFDAQTKIQ
                     ILAPLNKLDENKLSSLLQDGIQRLLINDEVVRLDEVKLPHSFPKNAAVYILIDRVTAK
                     QDEDNLHRLADSVQLAFAEGNGTCVVDIPDRKKQTFSNRFELDGMLFEQPTPQFFNFN
                     SPFGACPKCEGFGTILGIDEDLVIPNKNLSVYEDAVVCWKGETTGLWKKQLIKNASKA
                     DFPIHKPIIDLDKKQYQMLWNGCAYFQGIRDFFNDIESQTYKIQYRVMLARYRGRTVC
                     DACGGSRIRKDASYVKINNTDIGELLLMPIKNLNDFIQKIKLAESDKKIADRILLEIK
                     NRLHFMMDVGLDYLTLNRFSNTLSGGETQRINLTRTLASNLSGSLYILDEPSVGLHPS
                     DTTKLIAVLRHLRDLGNTVIVVEHEEEAIRSADYLIDVGPFAGTLGGQIVFSGNVDDI
                     IKHPNSLTTKYLTHQLEIELPKQRRKPVNFIELSGARQNNLKDIDVKIPLQAFTVISG
                     VSGSGKTTLVKQILYPYLKIQFDGAGNKPGLFKEIKGYKNKINRVELVDQNPIGRSSR
                     SNPVTYIKAYDAIREVFAQQPLSKIRGYQAKHFSFNVEGGRCDECEGEGEKVVEMQFL
                     ADVHLQCEVCRGKRFKSEILEVLYKEKAISDVLEMTVDEAISFFADKKEVVNRLMPLQ
                     EVGLGYVHLGQSSSTLSGGEAQRVKLASFLTKGSTKESVLFIFDEPTTGLHFHDINKL
                     LKAFNALIEIGHTVVVIEHNMDVIKSADWVIDLGPTGGEEGGYLLYQGTPEGLVKNTK
                     SLTGKYLKEKLS"
     gene            complement(14635..15420)
                     /locus_tag="PLF48_02115"
     CDS             complement(14635..15420)
                     /locus_tag="PLF48_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48145.1"
                     /translation="MNENLLDEDLLYQETDAKSVTDLINNGYETDVTGYINRGFKLFF
                     RDPGSYIAYLIVYIILAVALGLIMTFIPFGNVLSSIVMMPLFMGAIIVAKMLDKGESY
                     SFSNFFDGYKYFGEIAIASLIRIGVVILLAIVCLIPFGMKAIFNLSNLDEIMEDPITI
                     IQTLAIPVLILVVAYIVIFTFWLFAFMFIVFPKYKAWPALEASRKIISKRFFNWLGFC
                     ILLILVNFMGMLLLVIGLLITVPASICAMYLAFDEVCGVNIRP"
     gene            complement(15558..15728)
                     /locus_tag="PLF48_02120"
     CDS             complement(15558..15728)
                     /locus_tag="PLF48_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48146.1"
                     /translation="MRNKRSIIACGVIGGILTIPTLAMAKKLDRSAREPLGSVLLLCH
                     LRQLQVLLILQQ"
     gene            16195..>16767
                     /locus_tag="PLF48_02125"
     CDS             16195..>16767
                     /locus_tag="PLF48_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48147.1"
                     /translation="MNKKIKNYTALAAVSIAVLPMACKKDKDTTSTNDENIIDRTINQ
                     TLTATGSASEFGVEDSIDINSDGIFDFKLNTGGGAYYGSTYGYGYIHSLRTNTDLLTE
                     NATFGSETVKVLKTQNSGAKINAASSAWYYYGFTGYTYTGYTSVGFAGAGDKYIGFRL
                     GLSDGSHYGWMKVNQSSDFRTLKVIELAYHK"
BASE COUNT         5494 a         3013 c         2924 g         5336 t
ORIGIN      
        1 tttatctcat cttcattttc cggattaaaa taaatagcgg cgtctcctgc aatttcgggc
       61 aggcaagaag tattggaaca agccacagga atgccacaat gcatagcctc aataatcgga
      121 ataccgaaac cttcaaacaa actggggtaa atcatagccg cagcacaact taaatattga
      181 tagatttcct ctcgttggca gttttttatc cagattacct cttgtctggc gaccatattt
      241 tccaaataat ccagtaaatc ttcattcatc catccatcac tacctatcag caagagcttc
      301 atttgattac ttttactatg tttataagca ttaaatgctt taagcaatcg aagtatattt
      361 ttacgcgggt gaaaagtacc gataaaaata aaataaggga aatcaatacg tattccaggc
      421 ttacttccgg tgttgatttc tttaaaatct acgccattat agataacctc tattttgtct
      481 gaaaagatac tatactgatt gataatatca ttttttgtgt attcagaaac ggcaattatt
      541 ttatcggctt ttttgcaaaa ctgtggcgta tattttttgt aatagtctgc cactattttt
      601 gggataaact gagggtaatg ttcaaatgcc agatcatgga tggttagtag ttgtgagaca
      661 ttagtagata gcgagcaata cccgtcaggt gagacaaaca aatctatttt atcttgttta
      721 agtcgatagg gaatggcata ttcaaaccag gcataccaca ataaggcatg ccgggcaggg
      781 ggaaatacca tcttcggcac gatattctcg ctgtaaataa acgcatcatc aaactttctg
      841 tcgaaataaa aataaaactg atgttcgggc atgagcttca ccacacggct aaatagttct
      901 tgtgtataag tcccaattcc tgctaaggca tctttaatca gaaatcttgt attaacagcg
      961 attttcatgg agatttgaat ctatcatttt gtgttataaa cacttataca catattgtcg
     1021 aaagataagg ctttcgttgc atattatatt atgccgtttt atgtaaattt gcaaaaaaac
     1081 gaagtatgag atttgttgtt tccgcagggc agttattgaa aaatctgcaa aaaataagtg
     1141 gtgtaatcag taccaatacc gtattgccca ttctggaaga ttttttattt gatataaatt
     1201 ccaattccct gaccgttacg gctaccgatt tagacacgac tatgtctgtt tctatggaag
     1261 tagattccaa agaaaaattt aaggtggcta ttccggctaa aatattatta gacagtttaa
     1321 aggcattgcc tgaacaaccg attactataa cagtagatga aactacaaat gctattgaac
     1381 tgactacgga taacggtaaa tataagctga gtggcgaaag cagtgcagat tttccgaaag
     1441 agcctgcaac tgaagatgtg gaagaagtaa acttagcagc gtctatttta aataaagggg
     1501 tgaacaagac tttgtttgct gtgagcaatg acgaattgcg tcctgcaatg acaggtgtat
     1561 ttatccagtt gaatgagaac ggaattacat ttgtagctac tgatgcccac aagctggtga
     1621 aatttacccg ttctgatatt acaggcagtg atgtttcatt cattattcct aaaaaagctt
     1681 tgaatttatt aaaatcaaca gtgccgaata atgacactat agtaaaagtg cagtataaca
     1741 aatcaaatgc ttttttcact ttcgataata tcagtttgat atgcagacta atagatgcaa
     1801 agtacccgga ttataatgct gtaattccga aagaaaaccc gaatttactt tctgtaaaca
     1861 aagatgattt cttttcttct ttgcgaagaa cgtctatttt ctccaacaaa accactcacc
     1921 aggtggtatt gcgcatggca ggctcagagc tgaccgtttc cgcacaagat ttggatttct
     1981 caaatgaggc caacgagaaa ctgacttgcg actataaagg aaatgagatg gaaattggct
     2041 ttaatgcccg ttttttattg gaaatgctga gtgcggtaga ttctcctgaa attaatatcg
     2101 agctttctac accaaaccgt gcagggatta taaaaccgac cgatttatca gaaaatgaag
     2161 atatgctgat gctggtgatg ccggtaatgc taaacagtta ataaacacca accaaataga
     2221 atcttagttt taaaccgtgc agttatgtgc ggtttttttt atttgaataa ttaaagataa
     2281 gtagaatttg cagcttagtg cttttcgcgg ctaaaggtag tgcgttctat ctcatcggga
     2341 gaaatgtact catccgtttt ctcgcatttc accacgatat cccgctccca ttctttcaaa
     2401 gaaagattat tatcttcttt aagaatagag ttttgtttta ccttgctttt tggtttctta
     2461 tcgtttgctt tattaagttt tttcgttccc ataggttaaa gatacaattt ccgtaatttt
     2521 atcatccaga tttttaatta caaatcacat accaaatgag attatttatt ttaacttttt
     2581 tcgtgtctgt catttttacg tcttgtcaga ccgatgtaaa aacactaggt aagaaattta
     2641 cggaaagcaa cccgattagt gtagacgaat tgattagcaa gactaaagct gaaggtcatt
     2701 tagaccaggc acaggtaact ggcaaaattg acaaaagctg ccgatcggaa ggctgttggt
     2761 ttactataaa agacaataac ggtgctgagg tttatttgga tatcaaagac aaaggattca
     2821 agatgccgtt aaacagccct gaacgtcagg tggtagcctt agtaaatgcc gacacgagtg
     2881 tggcggcaga gagtggcgtt agcatagatg ttaaaggggt gatgtttaaa taagcgttaa
     2941 tagcttaaaa aataaagaaa gaggttgtta aaatgcaaaa aagccttatc tttgcacaca
     3001 cttttggata atttaagaac taaaggtcga ttggtttaat tttttaagct tatttactca
     3061 accctcttag attatttaga ttccgtagct cagcaggtag agcaatacac ttttaatgta
     3121 tgggtcctgg gttcgaatcc cagcgggatc acaggtgcca aaacctccgc ccttgtaact
     3181 cgttagttca agggcatttt tttttgcata aaaacaagag gcttcaatac aaagcctctt
     3241 ttccataaat catacgctat tagatttgtc ctaaataagc aaatggctta aatcgttgaa
     3301 tcacgtcaac caaatcagct attaataata agcaagtatc taaatcacga taacaaaatg
     3361 gtgcatctgt ttttatggat tcatttttaa ttatttcagg aatataaatt tgtgtacgaa
     3421 gtttattata gtcgaaatta tctgaaacca attttgcttc tgcccgagac atcgttctac
     3481 ctgtaccatg acagatagaa taatacacgg actgtatttt gttctttcct tttaccagta
     3541 ctacgtctcc caccatgttt gagggaatga cagtgatttc accgggtaat aatttagcag
     3601 caccttttct aattactaca aaatcatcaa taagttcaaa atgattatga ttcttgtcta
     3661 agattacttc agttcttcca aaatattttt ctacaatttt gagaacagct tttctattat
     3721 ctgcagccca attacacacg gactcaaatg tcaaatcaaa tttcttgggc tgatctacta
     3781 agtgatctac taatttagat tcatctctag agccggtgtg tattaaaaaa taaagataat
     3841 cgtcacgatc agaacgaagt gcatctaaaa aatggttccc agatcctaaa tcacctaact
     3901 tgcctttttg caacttcaag tctagatata tcttatccca aatctttgta tcgaaatcag
     3961 tatgtaaata tttagatttt gctaataaca ttccacaccc acaatcagat actgcaaatt
     4021 tgcgccaatc agtttgtttg gttattgcaa cagtaccggt aggcaatggt gatttgccgg
     4081 gacaagcatc aggaaaaaat attatctcat ctgcatgtaa gccatttgga atccaattgt
     4141 ataatgtatg taagggttcg gctgaaaaat tttgtatttt catattgtct gctttttaaa
     4201 attaataaat aaattattgt gcctttattg caaggcataa cctatttaaa tatttaattc
     4261 gcagattaca ttgtacattt gaaagtaact tgttattaaa gtatagacac aaaggtaagg
     4321 aaaattttga aatttaaatt accactaaac acatgattaa atttcaactg tcaatattgt
     4381 gttgcactac tcgactgata aattatttat cagcaaacaa tctttttact aaaaagcaaa
     4441 ttcgataata agataaataa attggattct ttatgttgat tacatactca gttatagaac
     4501 gataacattt tacaacttaa aaatcattta tttttgtcgc acatgaaacg ctatagacgc
     4561 atcatcctgc ttgtatttag cctgctatta attgctgccg ttatattgtt ttacatctgg
     4621 aacagaaaga tctattatcc taatttcggc attcgtattc cttccaatta tgagttgcat
     4681 ggcattgatg tatctcgcca ccaaggctgg ataaactgga aacaggtggc aacgatgaaa
     4741 gataaagata tccgcttgaa gttttgtttt atcaaagcaa ctgaaggggt ctcacatacg
     4801 gatatcttct ttaaaataaa ttggtatcgt tgtaaaaaaa atgggataaa gcgaggtgca
     4861 tatctctatt ttcatccggc taaaaacggt aagttgcagg cgaagcaatt tataaaaaca
     4921 gtacctctgg aaaaagggga tttcgctccg gttatcgatt ttgaagaaac aaatgatttg
     4981 tctaaatcaa agattataca aagccttaaa ctttgcattt ctacactgga aacgaaatac
     5041 gacaaaaaac cgattattta ttgcaacgtg gatttttaca ataagtttgt aaaggatgaa
     5101 ttgcccgact atccgatctg gattgcgcac tacaacagag catttgcacc ccgtataaat
     5161 gagcaatgga gtttctggca gcacaactgc aagggtcatg taaacggcat tgcatctgat
     5221 gtggacttta atgtattcag caataatgac agtgagttgt cgaaattgtg tttatagaaa
     5281 ctgaaattac cagaatttaa ttttccctgc ggcaaaccag cacatgcgca acaacatcca
     5341 tccattggta aagcgcgaga tattcgtatc gccataggta cgttcacggt agcgcaccgg
     5401 caaatcaata attttcagat ttaatttata agctccaaac agcaagtcaa aatcgccaaa
     5461 tgggtcaaaa tcgccaaaaa atgcacggtt gtttgccagt tcaatataat cttgtcttga
     5521 cataactttt gtgccacata aactgtcttt tatgggttgc tctaacagcc aagaaaacac
     5581 cttgctaaaa aatttatttc ccagggtatt taaaaatcgc atggcttctt tatccatcgg
     5641 gtatactaat cgcgatccgt tgataaattc tgctttacct gttgcaatgg cattataaaa
     5701 cttcggtagc tcttcaggcg gaacggtcag gtctgcatct aatatcatca agatgtcttg
     5761 ggttgccata ctgtaccctt ttcgtacggc gtcgcctttg ccttttccat cttgttgccc
     5821 tatttttatg tcgtgcgtgg aggcatattt tttctgaatt tcttgtattt tttcccaagt
     5881 gtcatctgaa gaatttccct cgatgaaaat aatttcctga tgctttccaa atttaggcaa
     5941 acgtaaaatg gcattctcga tattgccact ttcgttacgg gcaggaatac aaattgaaac
     6001 cgaatatttt tccttcacgc tttcttctgt ttcatgagga agaatgcggg caaaaataaa
     6061 ttcatttaag cataaggatt ttatcagcgg taattttgca atatatttat tgagtagcca
     6121 ggacaaaatc ggaatataaa caggaaataa cagtctgcgg ttagtgcggt aagtttcaaa
     6181 tccggataaa tacaacaaat tggagatatc ctgttcgttg agccagtttt gctgaggcga
     6241 tttctttttg attcccagcc attcaccgaa gttcaggatg ggctcccacc aatgattgta
     6301 gtaagaaata ataatccgtg tatgttgatg acaatttttt ttgatggaat aaaatacatc
     6361 ctgaatatta tcgaggtaac cgatgatgtt gttaaaaata atgacatcaa actttttatc
     6421 caagagcaaa ttctcggcat ccatcacata aaaatcaaca tctgtataat tcttttgcgc
     6481 agctattatc atattttcac taaaatcaat accggcttta tagctgcctt tcaagttggc
     6541 aatcgtttcg ccgtttccgc atccaatttc taatacagaa caatcatctt gcaagaagaa
     6601 attaaaataa ttgatgatat cctttccata ataagaaaag cgtttgcggt attgcctgta
     6661 agattgtgca atatcattaa aatgttggtg tatggtttgc ttgcgcgagt taaaattcat
     6721 aattattttc tacgtacaat aatagtttgg aataaagcta atttagaagc aaggggagac
     6781 aagatcattt ctgcgaaacc aactgcacca tacataaagt aaggcagcaa tgcaggcttt
     6841 gaaagtcctc cggacagtaa atattttaca ggagtatgaa atgtgatttt ggctatttcc
     6901 agttctggaa atttttttga aaataattcg gcatctctgt taaaaataat atacggtaat
     6961 gcaccattcg catcacttag tggacctttg ctggcaaatt tccattcttt aactgtttca
     7021 tcaaaaggtt cgtgatgaaa atttttatag ataattttag agaaccaagt agtggcaggt
     7081 tcaatcatat agataaagcc accagatgcc agtgtgcgtt cagcttcttt aaaaaaagct
     7141 tcattgtcgg gaatatgatg caaaacattg agcataaaaa tccctttcaa ggattgattt
     7201 tcgaaaggca tttcagttgc agaaaactgc atatcacaaa caggaagctt aattatatca
     7261 gaagttttta cctccgggta aacatcttta agaaagcccc cgcctgaacc aatttccaca
     7321 acatctcctt gaatggctat agacttagag taatttttaa attcgttgta ccaatctgaa
     7381 tgaattcgtt ttaaaaatgg cttagacaat actatttccc tgtgttgaag cgaagtttca
     7441 ggcagatcca gatgtggttg atgttctttc attgatttct aagccaaaaa taaataaatt
     7501 ccattatacc tacttcgtca aaacaggttg aagaggcttt gtcaacatag cactttttaa
     7561 agaatctatg taagtccaat tgattttata taaatcagat ttttctatat caccttcccg
     7621 atgcataaat tcaaatgcat ttggatattt ttgtacaata ttatacatat aacgctgcac
     7681 cgtatttata tactgatttt caatatatcg gtccgggtct agtcttagtt ctgaagtaaa
     7741 aatatgagta actttctgtt gtttaagcgg agttaaaagt gaatcagcat cttgtgccgg
     7801 cacattatag atgccataaa actttcttcc tgatgagaat acaaatgaca tcgggggttt
     7861 tctgcaagct atataatcct ctttgggcat attttctcca caccatttgc tcatggcaat
     7921 ataattcatc caatcagagg tatatccggc ataagggtta tccgtcatgt tatcaataaa
     7981 aattggaaga cgattcgcta tttttttact ggttgcagaa ataccactaa aaaacaaaac
     8041 aactacgaac aaaggtaaaa taaattgcaa cattgaattt attgacccat aataatatag
     8101 tatataaaac agactaaata aaataagtgg caaataaatc ataattaatc tagtttgccc
     8161 ccatgaagta tgtagcgtaa gaaaagtaac ggctaaaaat gaaataaagt agagtgaggt
     8221 aaacattaaa aggtcttgtt ttttgcgcca catcagtata aaagcccaag caatcagtcc
     8281 tactccaaca agggttaaga ttagattatt tggtgacatt tcttctctga aaccaagtac
     8341 atacatgaat ctggaggata tatatatttg agcgtttgta aaaaaacgat cgataaaccc
     8401 tgaaaaattt tctttaccca aagatgcatt atacgcatct ttttggaaca tcaaacggga
     8461 ttgagcttcg aattgtgaac catcaagttt ccagataaat ttcaatacca atttataaat
     8521 aaaataataa gtaccaaatg ccagaacaga tccaatagca tctattatct ttttttggta
     8581 aacaaaataa agaaatacta tgccaataaa tattaaagcg acatttcttg tcatcatttg
     8641 taaaaaaacc aagcctccta ttgccagata cataggtatt ttatccgtta tcgtaagcga
     8701 ttctgacttt aatttatcga atattttgaa aaggtaataa aatgtaagcc ccatgataaa
     8761 caaagaaaaa gtttctgcat aagtaagact ggcatacatc aagaaaggga aattaataac
     8821 cacaataaat agggctggaa tgagaattat atatggaata cgagttctga atgctttaaa
     8881 aaagaaataa atgcctaatg caaaaaataa tattgaaaac attttaagtg caattagatt
     8941 tacgccaaat atccgaatta ggaaactcag cacaattgga taaagtggcg catttgccgt
     9001 ataaaaatac ccaaagaaat tttttgaata atcaaatccg gcttcaatat acaatgcatc
     9061 atcatttgct gtagaaatct ttgtgtcaaa acagaaaaaa gaaaacacaa ccgcaattag
     9121 cagaagtgct atcaaatatt tacgttcatt ttttgcaaaa tgattttcca aaaagtcaaa
     9181 taaattaaaa ttcaacccat cttttgttac ttcttttttt gaaggtgcat ttaccggttt
     9241 tgtaacaact tgtttctcta tatttttctt attaggagtt tgttttgcca tcttttgatt
     9301 tttcaataaa taaatctata aatgtatatt catccatttt ttgtgtatgc ttaaaaatat
     9361 cattctttcc aaatggaacc aataaatcgc tcaatgtcat aaaaactgca aatccatttt
     9421 cccataaata attaaaaaac tgctcaaagt ctatcttcgc atttttcatt ccatagggat
     9481 agaattcggt gatgattttt aagttttcgt tttgcttgaa tatctctttc atgcctaaaa
     9541 atgcataata ttcgtagcct tgtatgtcta tttttataaa gtctactttg tgtccgggta
     9601 gtacatcatc cggcgcaact ccatctatct cggtagtaga tgtgtagttt tctgttggat
     9661 aggttttatg atctacattt aataaatcgg aatgatacaa cgtaattttt cctgtttttt
     9721 catttaccgc tttattaaaa aataccacat ttttaagatg acctgcattt ttcatcaggt
     9781 gatggaaatt tgttttatcc ggctcaaagg catatacttt cccattttcg cctaccaggg
     9841 cggaaaggat tttagtataa aaaccaatat tcgcaccgat atccaatacg atatctcctt
     9901 gtttgatata ttgctgcatt aaagcaatat cgtgtttatc ttgtctgcgt ttaaaataag
     9961 gataaatcac attgtaaaca ggaaatgcgt gtttatataa aaaatcgccg agttttatgg
    10021 ataaagcaga tggcatattt tattaattag ctgattagct gattagctaa ttaatttatt
    10081 tttttaatcc gatttctctg agtctctcgt ctaagtattc gcctgctgat ataggcgcat
    10141 aagcaagtgg atgttgttcg tctacacaat tttctaagca atccaatcgc acttcgctgc
    10201 ggggatgtaa aaaaaatgga atagagtaac ggtgtgtgtg ccatttttct tttggcggat
    10261 tgactacacg atgtgtagtc gattttagtt tattattgca caatcgctgc aacatatcgc
    10321 ctacatttac cataatctga tgaggttctg ctttggcatc gtgccaagta ccttcaacat
    10381 ccattacttg caatccatct gcactggcac ctaccaataa agtaatcaaa ttgatatctt
    10441 catgtgcttc ggcacgtatg gcactttccg gttctttcgt aatgggcgga tagtgtatgg
    10501 ctctcaaaat gctgttgcca ttatgaattt tcgcatcaaa ataattgaca tccaaatgca
    10561 gatattcggc aatagcacga agcaagtgtc ttccggatac ttcaaaattc ttatataata
    10621 atctgcccgt ttcattaaac tccggcaatt ctttcacttc tatattatcg ggataatcat
    10681 ccacaggcat tatttctcca tcttcaatat attgcccaat ttgccaaaac tctttcaaat
    10741 ctgccacttc actttgtttg gctttttctc taccgaaaga agtgtagcct ctttgtccgg
    10801 catattctat tttttcgtaa ttgagcttcg tatcagtcgg cattgcaaaa aatgtttcta
    10861 cctgactgta aaatttgtcg attaaatctt tatcgattcc gtgattttcg atggcagcaa
    10921 atccgatttc tgaataagct tttccaaaac cctgaataaa tttttgtctt cgactttcat
    10981 cacccgacaa ataatctgct aaatcgagta cgggaatata actcatgcgt tgttaatttg
    11041 ggataaatat acatgattct cttgacatgt aaaagagcgc atatttatta acaaccaaca
    11101 aaatacatat aagattacat aacaattatc ttgttgccaa tcattttttt caagttcaat
    11161 aaggcatagc gcattctgcc taaagcggtg ttgatatttg ttccggtcat ttccgcaatt
    11221 tctttaaaac tcatatcggc ataatggcgc aaaatcaaaa cttctttctg atcaggaggc
    11281 aatttatcta tcatatcccg cagggtatca gccgtttgct gacgcatgat tttgtcttcc
    11341 ctgttttcat caagcagtcc catcgaattt tgcactacat tactatccac atccaccgta
    11401 ttaggcatgc gtttattttt acggaaataa tccatacata aattatgggc aatcatcagc
    11461 acccaaggca aaaacttacc ttcttcatta tatctgccct gctgaagcga atcaattact
    11521 ttaaaaaaag tttcctggaa aatatcctcc gcctgagtac ggtctttcag cttaatcagg
    11581 ataaaagaaa ataccttacg ctggtacttg ttcaataatt gagcaagtgc ttgctggtcg
    11641 ccgttgatgt aattttgcac caactcattg tcagatagta cgattggata catgatctca
    11701 aaacttttgt tgttaaataa atagccgtcg tcatgtaaaa attgttcaat cttttctgat
    11761 ttttttatta atgttagttg ccagattaaa tataaataac ctattttaca gcgtgtttac
    11821 ctttaacagt atttaacgca agaatcgttc caatagtatg tcaaacgtta agaaattaac
    11881 taaatccacc caaaaaaata tccttataaa aggagcaaga acgcataatc ttaaaaatgt
    11941 agatgtagag attcccagag atcagcttgt tgtgataacc ggagtaagcg gcagcgggaa
    12001 atcttcactt actattgaca cgctttatgc tgaagggcag cgtcgctatg tggaatcctt
    12061 atcctcatat gcccgtcagt ttctaaaccg tatgaataag cctgatgtgg actatatcaa
    12121 aggcttgtca cctgccatag ccattgaaca gaaaacaaca acaggcacta cccgcagtac
    12181 ggtaggtaca cttacagaaa tttatgatta tttgcgctta ctatttgcta aagttggaaa
    12241 aacctattca ccagtttcgg gcaaagaagt aaagaaacaa gacatagctg atatacttgc
    12301 ttatattacc gattttgatg cacaaaccaa aatacaaatt ttagctccac taaataagct
    12361 ggacgaaaat aaattatcat ccttattgca agacggcata cagcgcttac tgataaatga
    12421 tgaagtggtt cgtttagatg aagttaaact tccgcattct tttccaaaaa atgctgccgt
    12481 ctatattctt atcgaccgag ttacggctaa gcaagatgaa gataatttgc acaggcttgc
    12541 cgattctgtc caattggcat ttgcagaagg aaacgggaca tgtgtagtgg atataccgga
    12601 taggaaaaaa cagacttttt ctaaccgatt cgaattggat ggaatgctgt ttgaacaacc
    12661 tactccacag ttttttaatt ttaattctcc ttttggtgct tgtccaaaat gtgaaggatt
    12721 cggtaccatt ttgggtatag atgaagactt ggtgatacct aataaaaatt tatctgtgta
    12781 tgaagatgcc gtagtctgtt ggaaaggcga aacgacagga ttatggaaaa aacaattaat
    12841 aaaaaatgca tcaaaagctg attttcctat tcacaaaccc attatcgatt tggataaaaa
    12901 gcaatatcaa atgctgtgga acggttgtgc ctattttcag ggcatacgcg atttttttaa
    12961 tgatattgaa tcccaaacct ataagataca ataccgggtg atgttagctc gatatcgcgg
    13021 acgtaccgtt tgtgatgctt gtggcggctc gcgtattcgc aaagatgcct cttatgtaaa
    13081 aattaacaat accgatatcg gtgaattatt actgatgccg attaaaaacc tgaatgattt
    13141 tattcaaaaa atcaaattag ctgaatctga taaaaaaata gctgaccgca ttttactcga
    13201 aataaaaaac aggctgcatt ttatgatgga tgtcggcttg gattatctta cattaaatcg
    13261 tttttccaac acgctcagcg gcggcgaaac gcaacgcatc aaccttaccc gcactttagc
    13321 cagtaattta tccggttcgc tttacattct ggacgagccc agtgtcggct tgcatccaag
    13381 tgatacaaca aaattaattg cagtattgcg tcatttacgc gatttgggca ataccgttat
    13441 cgtagtagaa catgaagaag aagccatacg cagcgcagat tacctgattg atgtagggcc
    13501 atttgccggc accctgggcg gtcaaatcgt ttttagcgga aatgtagatg atattatcaa
    13561 acatcccaac agtttaacga ctaaatatct gacccatcaa ctggagattg aacttcctaa
    13621 gcaaagacga aaaccggtta attttataga attatcaggt gcaagacaaa acaatcttaa
    13681 agatatcgat gtaaaaatcc ctttgcaggc attcaccgtt atcagcggag tttccggctc
    13741 gggcaagacc actttagtca aacagattct ttatccttat ctaaaaatac agtttgacgg
    13801 tgctggaaat aaaccgggat tatttaaaga aataaaagga tataaaaaca aaatcaaccg
    13861 ggtggagtta gttgatcaaa acccgattgg caggagttcg cgaagcaatc cggtaaccta
    13921 tatcaaagct tatgatgcta tacgggaagt ttttgcacag caacccttgt caaagatcag
    13981 aggttatcag gctaaacatt tttcatttaa tgttgaaggc gggcgttgtg atgagtgtga
    14041 aggtgaaggc gaaaaggttg tggaaatgca atttcttgca gatgtgcatt tacagtgtga
    14101 ggtatgcaga ggtaagcgtt ttaagagcga gatactggaa gttttataca aagaaaaagc
    14161 catctcggat gtattggaaa tgactgtgga tgaagccatt tctttcttcg cagataaaaa
    14221 agaagtggtc aacagattaa tgccattaca ggaagtagga ctgggctatg tacatctggg
    14281 acaaagcagt tctacactta gcggaggtga agcccaacga gtaaaactgg catcttttct
    14341 taccaagggc agtacgaaag agtcggtatt atttattttt gatgagccga caaccggctt
    14401 gcattttcac gatatcaata aactattgaa agcctttaat gcgctgatcg aaattggaca
    14461 tactgttgtt gtaattgagc acaatatgga tgtaattaaa agtgcagatt gggtaataga
    14521 cttgggaccc accggaggtg aagagggcgg ctatttgtta tatcaaggta cacccgaagg
    14581 actggtcaag aacactaaat cacttacagg taaatatctg aaagagaagt tgagttaagg
    14641 tcttatattt actccacata cttcatcaaa cgccagatac attgcacaaa ttgatgccgg
    14701 aacagtaatc agcaatccaa taacaagtaa gagcatcccc ataaaattta ccagtattag
    14761 taagatacaa aaacccaacc agttgaaaaa tcttttggat attatctttc ggcttgcctc
    14821 caatgccggc catgccttat atttcggaaa aacaataaac ataaaggcaa acaaccaaaa
    14881 ggtgaaaatg actatatatg ctacaacgag aattaataca ggaatagcca gcgtttgtat
    14941 tatagtaatc gggtcttcca ttatttcatc caaattcgat aagttaaaaa tggctttcat
    15001 cccaaaagga ataagacata caatggccaa aagtatcacg acacctattc ttatcaaaga
    15061 tgcaatggca atttctccaa aatatttata gccgtcaaaa aagttggaaa atgaataaga
    15121 ctccccttta tccagcattt tggctacaat aattgctccc ataaacaaag gcatcatcac
    15181 tatactcgaa agaacgtttc caaaaggaat aaaagtcata atcaacccca aagcaactgc
    15241 cagaataata tagactataa gatatgcaat atatgaaccc gggtctctaa aaaacaactt
    15301 aaatcctctg ttgatatatc cggttacatc ggtctcatat ccgttgttta ttaagtctgt
    15361 tacagacttg gcatctgttt cttgatatag caaatcttca tccagcaaat tttcgttcat
    15421 gcttattatt ttttcgacaa tatagttcta ttttttcaaa tattagtctg ttataactaa
    15481 ctagtttatt cagaaattac agcacaaagg tctcttctta gatttcttta ttactactgc
    15541 tctttttata cccattccta ttgctgtaat atcaaaagca cctgcaattg gcgcaagtga
    15601 catagtagca acactgaacc taaaggctct cttgcgcttc tgtctagttt tttagccatt
    15661 gccaaagttg gtatggttaa tatcccgcct attactccgc aagcaataat agatctttta
    15721 tttctcataa atttcttctc attccatatg cacatgtatt ttgaagaaaa agaattgata
    15781 tattgggcgt tcgtttttac agattttgcg ctcaataaaa tgaaatctga tttattattt
    15841 ttaattgtaa caaatttcct gatactatgc tcatttttaa gcatgattat gctcctttct
    15901 ttaaggctgt ccaaacattg ttgtgcatat atatccgact tgatgacaat tagcattaaa
    15961 caaaacaagc acttaaacac cagacaaatt ttactttagt ttttaataaa ataatatatt
    16021 aaatatatat tctttaatat tgatttataa aaacactgta ctcgtacctt atttaataca
    16081 aatatagtca taatttttag atattatcat tagacactct tacagaaaaa aatatttaat
    16141 aaattttatc cgttatttat gaaaataatt tatctttatc gaaaataaac cgatatgaac
    16201 aagaaaatta aaaactatac agcattagct gctgtttcga ttgctgtact accaatggct
    16261 tgcaagaaag acaaggatac gacttcgaca aatgatgaaa acattattga cagaacaatt
    16321 aaccaaaccc ttactgctac cgggtctgca agtgagttcg gtgttgaaga ttccattgat
    16381 ataaattctg atggtatttt tgactttaaa ttaaatacag gcggtggggc ttattatggc
    16441 tctacgtatg gatatggata tatacattct ttaagaacaa atactgactt attaacggaa
    16501 aatgctacat tcggcagtga aactgtaaaa gtattaaaaa cacaaaattc cggtgcaaaa
    16561 attaatgctg cgtcctctgc atggtattat tatggattta caggatatac ctacacaggc
    16621 tatacaagtg ttgggtttgc aggagcaggt gacaaataca ttggctttag attggggttg
    16681 tcagatgggt cacattatgg ctggatgaaa gtaaatcaat cttcagattt cagaacatta
    16741 aaagttattg aattagctta ccataaa
//
LOCUS       DAOQII010000022        16701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1644_length_16701_cov_5.010273, whole genome shotgun sequence.
ACCESSION   DAOQII010000022 DAOQII010000000
VERSION     DAOQII010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16701
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_16701_cov_5.010273"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLF48_02130"
     CDS             <1..697
                     /locus_tag="PLF48_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48148.1"
                     /translation="DNLQYYAYHSGKTEDLNHVVEHVLQKKAYEEYHLIGISMGGNML
                     MKYLGEEKWESVHTITSAVAVSVPMHYETTFPHLIKGWNQIYERRFTKQVKEKVKNKE
                     ARFPKAMDYKKIAEAKNLWEFTEFCIVPNHGFKNVHDYNLKVQASAYVHQIKTPILLI
                     QADNDPLLTKECYPISFAKKSHFFSLEITIGGGHVGFCEDIKAEHNWLEKRIVSYFTS
                     GDTIHHLHVKHEE"
     gene            complement(842..1279)
                     /locus_tag="PLF48_02135"
     CDS             complement(842..1279)
                     /locus_tag="PLF48_02135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNF48149.1"
                     /translation="MNNRIAYFRSHIGKEKWSFDYPMIEWMKAQVLEADEGIVKLQFT
                     VKDYMLNPLKIMHGGVASTMLDELMGAACFLMNKPVGYVTINMNVDYLSAAKENDILI
                     GEGKVIRAGRTTMHAEARIYNTEGKLLFKSSGNFMATQTPLPT"
     gene            complement(1423..3027)
                     /locus_tag="PLF48_02140"
     CDS             complement(1423..3027)
                     /locus_tag="PLF48_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNF48150.1"
                     /translation="MSNFENEIRRRKTFAIISHPDAGKTTLTEKFLLYGGAIQTAGAV
                     KSNKIKKTATSDFMEIEKQRGISVATSVMTFEYENTLINLLDTPGHKDFAEDTYRTLT
                     AVDSVILVVDCVKGVEMQTERLMEVCRMRNTPVIVFINKMDRDGKDPFDLLDELEQKL
                     FIKVRPLSWPINSGTHFKGVYNIYDNNLNLYRASGTKVEEEFVTIADLNDPALEKQVD
                     KRDADKLREDIELIDGVYEPFDVNTYLSGEIAPVFFGSAVNNFGVRELLNTFIKIAPF
                     PRPRNTDKRVVMPTEPKFTGFVFKIHANIDPNHRSRIAFLRVCSGKFERNEFYYHTRL
                     DKKLRFSNPVTFMASDKSIVEDAYAGDVIGLNDTGNFKIGDTLTEGEKLLFKGIPSFS
                     PEIFKELVNTDPMKTKQLEKGVLQLMEEGVAQSFNMHQSSTKIIGTVGELQFEVIQYR
                     LLNEYGASCTFKPLNYYKACWITSEDSDKINQFIRTKGNYMAWDKDNNPVFLATSEWI
                     LRLTQQDYPEIIFHTTSEFKKDEVVY"
     gene            complement(3100..3462)
                     /locus_tag="PLF48_02145"
     CDS             complement(3100..3462)
                     /locus_tag="PLF48_02145"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNF48151.1"
                     /translation="MKINKHYFKRFSILFLIECFIALFIHDNFIRSYVGDFLVVLMLY
                     FLLRTFTPFSPFISACIVLLYSFITEIAQYFQLIYHLKLEHSFLARIILGKTFSWTDM
                     LIYVLAFLTILLYNKRYD"
     gene            complement(3488..4249)
                     /locus_tag="PLF48_02150"
     CDS             complement(3488..4249)
                     /locus_tag="PLF48_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48152.1"
                     /translation="MPKSEATHTEIQKLLHLLRAENADDFSFLEKLTKDEIAKLRIRI
                     LENAHFEQDDIWKRLAGVAKFMPSFMNAKVGETVLGPMISANLSNYLPVKDAVSVMGY
                     MSSSYLAKVADYMIPEKSVELLNAIPMSILKKVTLELIKHKNYYTSAGFVDVLELDKI
                     VELSKVISDENDLIAISEFVENKSCIARIVEGFSDARICKIIETAYQSNRQEDVLSVF
                     MHLTSKETQRMLRLIASLPAAMKKKVLDDFEGRVG"
     gene            4354..5325
                     /locus_tag="PLF48_02155"
     CDS             4354..5325
                     /locus_tag="PLF48_02155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNF48153.1"
                     /translation="MKILVIRFSSIGDIVLTSPVIRCLKNQIADAEIHFLTKEPYAEI
                     VENNPNISKVFTIKAYTDEVIALLKAEQYDYIIDLHDNIRSRQVSAALKSKTFRYNKQ
                     RFRRFLLIRFKIDALHTHVVDRYFEAVSPLQVINDGNGLDFFIPEKGHLGAEKLPFTH
                     IAGYCVIAIGTKHFTKTIPLQKLEELCQKISIPIILLGDMHDAYNGTQLEKIDNFKIY
                     NACGKFSITQSASVIQRAKFVITGDTGMMHIAAALNKRIISIWGGTDKRLGFYPYQNS
                     SVNSVLIENTMLKCRPCSKHGLSACPKGHFNCMNQLDMQKVIAVIGK"
     gene            complement(5349..6119)
                     /locus_tag="PLF48_02160"
     CDS             complement(5349..6119)
                     /locus_tag="PLF48_02160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNF48154.1"
                     /translation="MKKLQLYIESFFNLDAIKIDWKITGTVLFAAGCLILAEYFDSIY
                     GIQHFFAIYFHKEINLYNLLPVPSRPVFQNLLVWGINCVITFLLLPVVFIKIILREKL
                     SNFGLNLHLEKRFILLYTIMFLVMIPVLIYLSYTAAFLEKYPFFKIQSKAELPLFLKW
                     EIIYILQFVGIEFFFRGLLIHAFKHKFGYYSILFSVIPYCMIHFGKPLPETIGAIFAG
                     LILGFLSLNTGSIILGILLHCGIAVTMDFLALGALFSQ"
     gene            complement(6186..6839)
                     /locus_tag="PLF48_02165"
     CDS             complement(6186..6839)
                     /locus_tag="PLF48_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF48155.1"
                     /translation="MKKWMLIILFLPLLLQARPRLFSTGSRLKFQFGGSAGYNLNYFI
                     YRTDAQRDVSSGYQAGVFFRISRSRVFGQMEFNFRGSEVRLHNENLVLNSGTLLHVDK
                     LSFKYHTFSIPLIFGGYAIKKPLYKIRFYNGMVTDFILKTNVIYTENNSDKIYKLPRK
                     EKRDALYPVQLNYMLGAGADIAFFTLDIRYNLGFRNFYKEEFRTQTHIFEFTLGLIF"
     gene            complement(6844..8484)
                     /locus_tag="PLF48_02170"
     CDS             complement(6844..8484)
                     /locus_tag="PLF48_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF48156.1"
                     /translation="MSIKLYKKLLIVITISLTAVIFFVSCYPLSTGAIKWRIKWDKKE
                     VASKKSFLQTQTSCSVIRPNIILIVADDLGLNEVSSYDNTALQTPNIDQLAKDGIRCT
                     DAYVTAPICAPSRCAILTGRYQQRCGFETQPTEFYPENFVEYMLGKKFAQRDSSWVVA
                     GKHDYPFPWQIAKQGMPPTEITIAELLKKQDYATAIIGKWHLGLNEDINIPNKFGFDY
                     QFGIYGSQTLYAESVNAPDIINYEHKSFTSKYMWQMGRKDVAMIYENNKKIKHQPGYL
                     TDVFRDKAIRFIEKNKDRPFFLYLPFTAPHEPYQAPLDLYSEAYVQTHEKGKAVYGAI
                     VRAMDNAIGAVYQKIKDLKLDENTLIIFLSDNGNANYGRVSSCAPLQGGKFTFFQGGV
                     KVPVIYCWKNQLPQNRVYPKSVSALDVFSTIAAATNTTLPNDRVIDGVNLLPYFKQEN
                     MTEPHPVLFWKADKMYAVKSGDYKLLMDLQQGWTELYDLKNDPYEKHNISADHPEKTE
                     ELRQVILAWSKQLPNRPLWPRIMDYKIKINGKIYYFPV"
     gene            8515..9144
                     /locus_tag="PLF48_02175"
     CDS             8515..9144
                     /locus_tag="PLF48_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNF48157.1"
                     /translation="MKIEIKKEIANAITHGLGFITFAALIPIMFVRYAHLKLDNKLTG
                     LIFFAIGLVLVYASSTIYHAIQHKKAKHILRIADHISIYYLIAGTYTALIVLCIPLEK
                     VKIFLTVLWVVVGAGTIKKIFLTGKYDTLSTIIYVALGCLGLLEINNILHFVPGKALL
                     FILLGGLSYLLGVIFYAWKKYTYHHAVWHLFVLNGSIMHFFSVWYAMGK"
     gene            9256..10134
                     /gene="folD"
                     /locus_tag="PLF48_02180"
     CDS             9256..10134
                     /gene="folD"
                     /locus_tag="PLF48_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNF48158.1"
                     /translation="MILLDGKRISADIKQEIKEEVLQLKAAGKKAPHLAAILVGNNPA
                     SEAYVANKVKSCKEVGFDSTLITLENVATEEEVLATIQQLNEDAAIDGFIVQLPLPKH
                     INEEKILLAIHPEKDVDGFHPLNVGRMVLNLPAYVSATPKGILEIIKRYNIETEGKRC
                     VVIGRSNIVGLPMSILMGKKNKFANSTVVLCHSKTNDLKAETLQADIIIAALGIPQFL
                     KADMVKEGAVVIDVGISRIEDPSRKSGFRLVGDVDFEEVSKKCSYITPVPGGIGLMTV
                     ASLMQNTLSACKKEFY"
     gene            complement(10249..11229)
                     /locus_tag="PLF48_02185"
     CDS             complement(10249..11229)
                     /locus_tag="PLF48_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48159.1"
                     /translation="MKKLFFLSLLSLTISSVWAANVMLTVHLKNGDIISGKSTLNKIA
                     ITTAYGNLTIPIDQISALQFGIPSDHSKDAAVVADLTKLNAAKGLEAKPIYDKLLAMG
                     TPVLSTVKTFSESTNYSISENENYTIEQLLDELYKIANLSSADPIEDAITFGNNNYIE
                     GNITFSDILLQSDYGNLTFKKDKIESLDISMIDESTILGNGQFRLKANTQISGNEKQG
                     WVNTGITLKEGDKFTISATGKIILKSLSGGAFNPDGYVSGTKDGAYNEDAEIKYGAVV
                     YKIGENGTYLGAGSKFEGTAESSGPLFISIYETVYDKSNTGSYTVTVKKK"
     gene            complement(11339..12826)
                     /locus_tag="PLF48_02190"
     CDS             complement(11339..12826)
                     /locus_tag="PLF48_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNF48160.1"
                     /translation="MKILSANQIREADAYTIKNVPIASIDLMERAAKKWTALFLKDYP
                     DKKQTKYIFCGPGNNGGDGLAIARLLLHEKHAVNVFILHSSQYSADFQLNLQRLKHYK
                     CILSANDFPKLDKEDIVIDALYGSGLNKPINGLSAALINYLNEQESSIISVDIPSGLY
                     TEKQSGMPIIQAQKTYTFQFPKLSFLLPPSGNTVGNFKVADIGLHPDFISKINTPYFY
                     TNKKEIKALLKTRNKFSHKGTYGHALLVAGSHGKMGAATLCAKAVMRSGAGLLTAHIP
                     GCGYSILQTALPEAMCICDDNNMHISGIPIQMTYAAVAIGPGLGTHQDTASALKSFLQ
                     ANKTPCVIDADALNLIAQHKAMLKLLPENCILTPHPKEFERLTGTWTDDFDRLQKLQS
                     FSKKHKVITLLKGAHTAIADVSGNVYFNSTGNPGMATAGSGDVLTGIICGLLAQGYSA
                     LESSILGVFLHGSAADLALKKQSEESLIASDIIKNLGRAFKHLKK"
     gene            13276..16413
                     /locus_tag="PLF48_02195"
     CDS             13276..16413
                     /locus_tag="PLF48_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF48161.1"
                     /translation="MSGLLNHMRFNFLSLLLLAYLSGFALDVSLFKFKTLDIKDGLSQ
                     NSINYIFQGSKGYMWFCTNEGLNLYNGYSFEVFKNQIDNSNSLVSNFTQCMAETSDGV
                     FWIGTERGISVFMPDKKIFVNTESPLQIKRIIAEDDKAVWVQTNYSVFRAELVQIGKK
                     GLSPKDYAINIKFYYDYDEILYKDKEGKILLSSKNDLLYNYVPKSQTVVLRSSSKIWA
                     ALSAKKINSVVQDNQFNYWIATNSGVFKCDKNFNDIDTFSANRKKLPDLQSKINDLSV
                     DKAGNIYMATSQNGLIIYDAKQAKFYNYQYDIFDLTGLPDNKLLSVLVDKSQTLWVGS
                     KGAGISAYSPYKYKFKLITQEPFKTSWLTNKYILAIEKDKTGNIWVGTDGGGLFRYDV
                     KKNLFTNWRNNSAFSSLSNDVVQDILIDKKGTLWIGTLNGICKFSTSGNNFIRYYFTA
                     NNAHENLTVNRYSNIRLFEKSNGELYAFDDNNIYKFNAIRQEFDLLNIMYYSRHFIPR
                     FITEDDDGTLWIASNAGISHLNTAEGFIDIDALKKLNEKYFKNGRVYALLKEDADRFW
                     VATGNNGIYLFNKKTFSIDVNYTEKSGLSNNYVYSILKDNSGKLWMSTNRGISVFDPI
                     SKTFKNYDVNDGLQSNEFNGGAFLKTDDDEIYFGGVNGFNIIQANEVPFNAYKPSSNI
                     ISVKANDIPVDFDRYSQHPKRVKFSNDQDNIEFEFSSSDYANPPKNMFQYKLEGYDKN
                     WTTSNRNYVKYAKLPYGNYTLLVKASNNDAVWSDNISRFEFRIKPMFWQTILFKILFV
                     IASLFLLYRFIKNTIRSARMKEIEKARKEKQKTIYEKQLAEIKLKALVAQMNPHFIFN
                     CMNSIQAMILSDKNMEASTYLTKLSRLVRAVLENSVKTFIPLSEIIENLKLYLELESL
                     RFDKKFHYDLKVENLDIYSVEMPSMLFQPYVENAIWHGLLKKEGEKNLQIHFFKRDNY
                     CICEITDNGIGRTKAAELNLKKQHKSLGTVITKDMFETLHKIRDSKYTVDIIDLYDEH
                     NEATGTKVKIEIEIN"
     gene            complement(16410..>16701)
                     /locus_tag="PLF48_02200"
     CDS             complement(16410..>16701)
                     /locus_tag="PLF48_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNF48162.1"
                     /translation="DIGSVVMPDAELKFFMTADLDVRTKRRQDELLAKTGKLFSLEEL
                     KSNLIHRDKIDSTREDSPLTQVDDAIVIDTTHLTLEKQIELIVDKAKGLINA"
BASE COUNT         5337 a         3020 c         3058 g         5286 t
ORIGIN      
        1 ggacaatctg caatattatg cctatcactc cggtaaaacg gaagatttga atcatgtcgt
       61 agaacatgtt ttgcaaaaaa aagcgtatga agaatatcat ctcattggta tttctatggg
      121 cggcaacatg ctgatgaaat atctcggtga agaaaaatgg gaatccgttc atactattac
      181 ttctgccgtt gccgtgtcgg tgcctatgca ttacgaaact acatttcctc atttgataaa
      241 aggatggaat caaatctatg aaagacgatt taccaagcaa gtaaaagaaa aagtaaaaaa
      301 taaagaagca cgttttccta aagcgatgga ttataaaaaa atcgctgaag ctaaaaattt
      361 atgggaattt acagaattct gcatcgtgcc taatcacggt tttaaaaacg tgcatgatta
      421 taatttgaaa gttcaggctt ctgcatatgt acatcaaata aaaacaccta tactgctgat
      481 acaggcagat aatgatccgc tattgactaa agaatgttat cccatttctt ttgccaaaaa
      541 atcgcatttt tttagtttgg aaatcaccat aggcggtgga catgtaggtt tttgtgaaga
      601 tatcaaagct gagcataact ggttggaaaa acgtattgta agctatttca catcaggaga
      661 taccatacat catctgcatg tcaaacacga agagtagttt attgattaat gttgaatgtt
      721 aaatgctgaa tggttaatga ttaatgttga atgattaatg ttgaatgtta tatgctcaat
      781 gttgaatgat acattgtacc aatgtactaa taaccaataa ctaatgacca atcaccaatc
      841 actaagtagg caaaggggtt tgtgtagcca taaagtttcc tgaagattta aacaagagct
      901 tgccttccgt attataaatt cgtgcctcgg catgcatcgt ggttctccct gctctaatca
      961 ctttgccttc accaattaga atatcattct ctttggcggc actcaaataa tcaacattca
     1021 tattgatggt aacatatccc acgggtttat tcatcaaaaa gcaagctgca cccatcagct
     1081 cgtcgagcat ggtagatgct acaccgccat gcattatttt gagcggattg agcatgtagt
     1141 cttttaccgt aaattgcagc tttacaatac cttcatctgc ttccaaaact tgtgctttca
     1201 tccattctat catcggataa tcaaacgacc atttttcttt cccgatatgg cttctgaaat
     1261 aggctattct gttgttcatg acattatttt tatcttattt tatacaacaa aaatagttga
     1321 ttttattttg tttaaaaacc acctccttta gttcctcctc cttagttaag gcggtggata
     1381 aataatgaaa tcaactatta tttttgcttg ttgctaaatc catcaataaa ccacttcgtc
     1441 ttttttgaat tctgatgtgg tatgaaaaat gatttccgga taatcttgtt gtgtaaggcg
     1501 caaaatccat tcgctggtag ccagaaaaac aggattattg tctttatccc aagccatgta
     1561 attgcccttg gtgcggataa actgattgat tttatcagag tcctcactgg taatccaaca
     1621 tgctttataa tagttcaatg gtttaaacgt acaggatgcg ccatactcgt tgagcagtcg
     1681 gtattgtatt acctcaaact gcagttcgcc cactgtgcca attatttttg tactcgactg
     1741 gtgcatattg aaactttgtg ccacgccttc ttccatcaat tgcagtacgc ctttttccaa
     1801 ttgtttggtt ttcatcgggt cagtatttac caactcttta aaaatttcgg gagagaaact
     1861 cgggatgcct ttgaaaagca atttttcgcc ttcggtaagt gtatccccga ttttgaaatt
     1921 gcccgtgtcg ttcaagccaa tcacatcgcc ggcataagca tcttccacga tgcttttatc
     1981 cgatgccatg aaggttacag gattagaaaa acgcagtttc ttatctaagc gggtgtggta
     2041 ataaaattca ttgcgctcga acttacccga acatactctc aaaaaagcaa tgcggctgcg
     2101 atgattcggg tcgatattgg catgtatttt gaatacaaag cctgtaaact ttggttcggt
     2161 aggcatcacc acccgcttat ctgtatttct cggacgagga aagggggcga ttttaatgaa
     2221 cgtattcaac aactcgcgga caccaaaatt attaacggca gagccaaaaa acacgggagc
     2281 aatttcgccg ctcaaatacg tgtttacatc aaaaggttca tatacaccat caatcaattc
     2341 tatatcttca cgcaatttgt cggcatctcg tttgtccact tgtttttcga gagcagggtc
     2401 gttcaagtct gctatggtta caaattcttc ttccacttta gttcctgaag ctctatacaa
     2461 gttcaagtta ttatcataaa tattgtaaac acctttaaaa tgcgtgccac tattgatggg
     2521 ccacgaaagc gggcgcactt tgataaacaa tttctgctcc agttcatcga gcaagtcgaa
     2581 cgggtctttt ccgtctctgt ccattttatt gataaaaacg ataacgggcg tattgcgcat
     2641 gcgacacact tccatgagcc gttccgtttg catttccacg cctttcacac agtccaccac
     2701 taaaataacg ctatccacag ccgtcaaagt gcggtaggta tcttccgcaa aatctttatg
     2761 gccgggagta tccaataaat tgataagtgt gttttcgtat tcaaatgtca tcacggaagt
     2821 agccaccgag ataccgcgtt gtttctcgat ttccataaaa tcggaagtgg cagttttttt
     2881 tatcttgttc gattttacgg cacctgcggt ttggatagca ccgccataga gtagaaattt
     2941 ttcggtaagc gttgttttac ccgcatcggg gtgagagatg atggcaaacg tctttctgcg
     3001 tcggatttca ttttcaaaat ttgacatagg gcgcaaaggt acggaaatga gcgggaaatt
     3061 tggagatttg ataatttgcc aatttgataa ttttagatat caatcatacc ttttattata
     3121 caaaagaata gtcaggaatg ccaatacata gatcagcata tctgtccatg aaaatgtttt
     3181 gcctaatatg attcttgcta aaaaagaatg ttctaatttc agatgatata taagttgaaa
     3241 atattgtgca atttctgtaa taaaggaata gagcagaacg atacatgcgg atataaatgg
     3301 actaaaaggt gtaaaagttc taagcaggaa gtataacatc aataccacca aaaaatcgcc
     3361 aacgtaagaa cgaataaaat tatcatgaat aaataaggct atgaagcatt ctattaaaaa
     3421 caaaatagaa aagcgcttaa aatagtgttt gttaattttc atttttaaga atatcaagtt
     3481 tatctcctca ccccactctt ccctcaaaat catccagcac tttctttttc attgctgccg
     3541 gcagggatgc tattaagcgc aacatgcgtt gcgtttcttt cgaggtaagg tgcataaaca
     3601 cactcaacac gtcctcctgc ctgttcgact gataggcggt ttctataatt ttacaaatac
     3661 gggcatcgct aaagccttct actatacggg caatgcatga cttgttttcc acgaattccg
     3721 aaatagcaat caaatcattt tcatctgaaa ttacttttga taattctact attttatcaa
     3781 gttccaacac atccacgaat cctgccgaag tataataatt tttgtgctta atcagttcta
     3841 aagtaacttt tttcaagata gacataggga tggcgttcag taattctaca gacttctcag
     3901 gtatcatgta atctgccact tttgccaaat acgagctcga catgtatccc atcacggaaa
     3961 cggcatcttt caccgggagg taattgctga ggttggctga tatcatcggt cccaacacgg
     4021 tttcgcctac cttggcattc atgaaactgg gcataaactt ggctacacct gccagcctct
     4081 tccagatatc gtcttgttca aaatgggcat tttccaatat gcgaatgcgc aattttgcaa
     4141 tttcgtcttt cgtcagtttt tccaaaaaag aaaaatcatc tgcattttca gcacgtagca
     4201 aatgcaacag tttttgtatt tcggtatgtg tggcttcact cttcggcatg attttaattt
     4261 agtgaattag ataagcagct aaatatcgta aatctatttc ttttttcttt cggcatgtaa
     4321 atcttatttt tgaataaaat aaaattttta aggttgaaaa tactagttat acgttttagt
     4381 tctattggcg atatcgtttt gacgagtccg gtaattcgtt gcctgaaaaa ccagattgca
     4441 gatgcagaaa ttcatttttt gactaaagaa ccttatgctg aaattgtaga gaacaaccca
     4501 aacatttcta aagttttcac gataaaagca tatactgatg aagtaattgc tttactcaag
     4561 gcggaacagt atgattacat tatcgacttg catgataaca tccgctctcg ccaggtttcg
     4621 gcggcattga agtcgaaaac atttcgatac aacaaacaaa gattcagacg gtttttgctg
     4681 attcgattta aaatagatgc acttcatact catgtagtcg atcgttattt tgaagcggta
     4741 agccccttac aagtgattaa tgacggaaat ggactggatt tttttatccc ggagaaaggc
     4801 catttaggtg cggaaaaatt gccgtttaca catatagcag gatattgcgt tatcgccatc
     4861 ggcaccaagc attttaccaa aacaattcca ctacaaaaac tagaggaatt gtgccaaaaa
     4921 ataagcattc ccattattct gttaggcgat atgcacgatg cttacaatgg aactcaatta
     4981 gaaaaaattg acaattttaa aatttataat gcctgcggga aattttcaat aacacaatcg
     5041 gcatctgtta tacaacgtgc taagtttgtc atcacaggag ataccggtat gatgcatatt
     5101 gcagccgcct tgaacaaacg cattatttct atttggggcg gtactgacaa acgactcggt
     5161 ttttatccgt atcaaaactc aagcgtaaat tcggtgctca tcgaaaacac tatgctgaaa
     5221 tgcagaccat gcagcaagca tggcttaagt gcatgtccca aagggcattt caattgcatg
     5281 aatcaactgg atatgcaaaa agtaattgcc gtaattggaa aataaggtgt caattattga
     5341 ttgttttatc attgagaaaa caaagcaccc aaagctaaaa aatccatagt gacggcgata
     5401 ccgcaatgca acaaaatgcc taaaataata gaacccgtat tcaaacttaa aaatcccaat
     5461 attaatcctg caaatatagc gccaattgtc tcgggtaagg gcttgccaaa atgaatcatg
     5521 cagtagggga tgacagaaaa caaaatactg tagtatccga atttatgttt gaatgcatgg
     5581 attaataatc cgcggaaaaa aaattctata cctacaaact gtaagatgta aataatttcc
     5641 cattttaaaa ataagggaag ttcagctttg ctttgaattt taaaaaaagg atacttttcc
     5701 aaaaatgcgg cagtgtatga taagtagatc agcacaggta tcatcactaa aaacataatt
     5761 gtatacaaga ggataaatct tttttccaaa tgcaaattca atccaaaatt gcttagtttt
     5821 tctcgtaaaa taattttaat gaagactaca ggaagcaata aaaaagtgat gacacagttg
     5881 ataccccaaa ccaataaatt ttgaaaaaca ggacgagaag gtacgggaag taaattatac
     5941 aagttaattt ccttatgaaa atagatggca aagaaatgct gaataccata aatgctgtca
     6001 aaatattctg ctaaaatcaa gcaacctgct gcaaacagta ctgtgcctgt tattttccaa
     6061 tctattttaa ttgcatctaa attaaagaat gactcaatat ataattgtaa ttttttcaat
     6121 ttgcaggttt tgatttaatc cggtttaaaa taactaatgt gctactcttt aacttatcac
     6181 atatcttaaa atattaagcc taacgtaaac tcaaaaatat gcgtttgcgt gcgaaattct
     6241 tctttataaa aattgcgaaa gcccaaattg tagcgaatat ctaaggtgaa aaatgcaata
     6301 tcggcgcctg cgcccaacat ataatttaac tgtacaggat ataaggcatc ccgtttttct
     6361 ttacgaggaa gtttataaat tttatccgaa ttattctctg tataaatgac attggttttc
     6421 aaaataaaat cggttaccat gccattgtaa aatcggattt tatacaaagg cttttttatg
     6481 gcatagccac caaatatcaa aggaatggaa aaggtatgat atttaaaaga cagcttatcg
     6541 acatgcaata atgtgccgga attgagaact aaattttcat tgtgcaatct gacttcactt
     6601 ccacgaaaat taaattccat ttgcccaaag actctggaac gactgatacg aaaaaacacg
     6661 cccgcttgat agccactgga tacgtctctc tgtgcatccg tacgataaat aaaataattc
     6721 agattatatc ctgcacttcc gccaaattga aatttcaagc gactgccggt gctaaatagc
     6781 cttggtcgcg cttgtaaaag aagcggcaaa aacaagataa tgagcatcca ttttttcata
     6841 gccttacacc ggaaaataat aaatttttcc gtttattttt attttataat ccataatccg
     6901 aggccataat ggccgatttg gcaattgctt agaccaagct aaaatcactt gccttaattc
     6961 ctctgttttt tcaggatggt ctgctgagat attgtgtttc tcataagggt catttttcaa
     7021 atcgtacaat tctgtccaac cttgttgtaa atccatcaat aatttataat caccactttt
     7081 gaccgcatac atcttgtctg ctttccaaaa taaaaccgga tgtggttccg tcatattttc
     7141 ttgcttaaaa tagggcaata aattgactcc gtctatcacc cggtcattcg gtaaagttgt
     7201 atttgtagcg gctgcaatag tgctgaatac atctaatgca gaaacggatt ttggatatac
     7261 cctattttgc ggtaactgat ttttccaaca gtatatgaca ggcactttca cgcccccttg
     7321 aaaaaaggtg aattttcctc cttgcaaagg tgcacaagaa cttactctgc cataattggc
     7381 atttccatta tcactcaaaa aaataatgag cgtgttttca tccaatttta aatcttttat
     7441 tttttgatac actgctccaa tagcattatc cattgctctg actatcgccc cgtaaactgc
     7501 cttgcctttt tcatgtgtct gcacataagc ctcagaatac aaatcaagcg gtgcctgata
     7561 gggctcgtgc ggtgcggtaa atggcagata taaaaaaaat ggacggtctt tgtttttttc
     7621 tataaaacgg atggctttat cacggaatac atcggtaaga tatccgggtt gatgtttgat
     7681 ttttttattg ttttcataaa tcatggctac atcttttctg cccatttgcc acatatattt
     7741 tgaggtaaac gatttatgtt catagttgat gatgtcggga gcatttacac tttcagcata
     7801 taaggtctgc gagccgtaaa tgccaaactg atagtcaaat ccgaatttat tcggaatatt
     7861 gatgtcttca ttcaagccta aatgccattt gccaataatg gcagtagcat aatcttgttt
     7921 ttttagcaac tcagctattg taatttctgt aggtggcatg ccttgtttag ctatttgcca
     7981 cggaaaagga taatcatgct tgccggcaac tacccagctg gaatctcgtt gagcaaattt
     8041 tttacccagc atatattcca caaaattttc aggataaaat tcggtgggtt gtgtttcaaa
     8101 cccgcaacgt tgttgataac gaccggtaag tatggcacag cgcgatgggg cacagatggg
     8161 tgcggttaca tacgcatcgg tacaacgtat tccatctttt gctaattgat cgatattggg
     8221 tgtttgcaat gcagtattgt cgtagcttga tacttcattt aagcccaaat catcggcaac
     8281 tattaaaatg atattgggtc gtatgacaga acaagaggtt tgagtttgta aaaatgattt
     8341 ttttgatgcc acttcttttt tatcccattt gatacgccat tttattgctc cggtactcaa
     8401 tggatagcag gatacaaaaa aaataacagc cgttaacgat atcgtaatga ctattagcaa
     8461 ttttttgtat agttttatgg acattagaaa gaaattagca agctaaaaat aaaaatgaaa
     8521 atcgaaatta aaaaagaaat cgcaaacgct attacacacg gtttaggttt tataacattt
     8581 gccgcattaa ttccgattat gtttgtgaga tatgcgcatc ttaaactcga caataaactg
     8641 acaggattaa tcttttttgc catcggtttg gtgctggttt atgcgagttc aactatttac
     8701 cacgccattc aacataaaaa agcaaaacat attctgcgca ttgccgacca tatcagcatt
     8761 tattatttaa ttgccggcac atatacggca ctaattgtgt tgtgtattcc tctggaaaag
     8821 gtgaaaatat ttttgactgt tctttgggtg gttgtaggcg caggaacaat taaaaagatt
     8881 tttctaaccg gaaaatatga cacactttcc accattattt atgttgcatt aggttgttta
     8941 ggattattgg aaattaataa tatactccac tttgtacctg gcaaagcctt attatttatt
     9001 ctgttgggcg gtctgagtta tttattgggt gttatatttt atgcatggaa aaaatacact
     9061 tatcatcatg ctgtgtggca tttgtttgtt ttgaatggta gtattatgca cttctttagt
     9121 gtttggtatg ccatggggaa atagatatct tctgtttttt tatctatact tatagcaaca
     9181 tcttgctgat aatattttgc taaaattaca tttcactaat ctttgaaatc gccgtaattt
     9241 tgcaaaaaaa atagtatgat attattagat ggcaaacgca tctctgctga cattaaacaa
     9301 gaaataaaag aagaagtttt acaactgaaa gcagcaggaa aaaaagcacc gcatctcgca
     9361 gccatattgg tgggcaataa tccggcaagc gaagcttatg ttgccaataa agttaaatca
     9421 tgcaaagaag taggatttga ttccaccctc atcacgttgg aaaatgtcgc tacagaagaa
     9481 gaagtactcg ctactattca gcagctgaat gaagatgcag ctatagacgg ttttattgtg
     9541 caattgcctt tacctaagca tatcaatgaa gagaaaatac ttttagctat acatcctgaa
     9601 aaagatgtag atggttttca tcctttaaat gtaggacgta tggttttgaa tctgcctgct
     9661 tatgtatccg ctacgcctaa gggcatttta gaaatcataa aacgctataa tatagaaacc
     9721 gaaggtaaac gctgtgtagt cataggccgc agtaatattg tgggtttgcc gatgagtatt
     9781 ttgatgggca aaaaaaacaa gtttgccaat agtacagtgg ttttatgcca ttccaaaacc
     9841 aacgatttga aagcagaaac tcttcaggca gatattatca ttgcggcttt gggtattccg
     9901 caatttctga aagccgatat ggtaaaggaa ggagcggtgg tgatagatgt gggcatttcg
     9961 cgcatagaag accccagcag aaaatcggga ttccgtttgg taggagatgt ggactttgag
    10021 gaagtgagca aaaagtgcag ttatattact ccggttccgg gtggcatagg cttaatgacc
    10081 gtagccagct taatgcaaaa tacactcagc gcctgcaaga aagagtttta ttgagaaatg
    10141 cttgaaaatg atatttcaac tactataaac cacacacaca actccattgc cctcaaaaaa
    10201 aaccaatgcc taatcaccaa tcacaaatga ctaatgatca atcctcaatc acttcttctt
    10261 cacagttaca gtatagcttc cggtattgga tttgtcataa actgtttcgt aaatagaaat
    10321 aaataaagga cctgaacttt ctgccgtacc ttcaaatttt gaaccggctc ccaaataagt
    10381 accgttttct cctattttat aaactacagc accatatttt atttcggcat cctcgttata
    10441 ggcgccgtct tttgtaccac tcacatagcc gtcaggatta aatgcgccac cggataaact
    10501 tttcaaaatg atttttcctg tggcagaaat cgtaaattta tctccttctt ttagtgtaat
    10561 gcccgtattt acccagcctt gtttttcatt tcccgaaatt tgcgtatttg cttttaaacg
    10621 aaattgaccg ttccccaaaa tggtagattc gtcaatcata ctaatatcca ggctttcaat
    10681 tttatctttt ttaaacgtca ggtttccata gtctgattga agcaaaatat cactaaaagt
    10741 aatattccct tcaatatagt tattattgcc aaaagtaatg gcatcttcga ttgggtcggc
    10801 acttgacaaa ttagcaattt tatacagctc atccaacaat tgctctatgg tatagttttc
    10861 attttctgaa atagaataat tcgtactctc tgaaaaagtt tttacagtag ataaaacagg
    10921 agtacccatg gctaatagtt tgtcatagat tggtttggct tctaatcctt ttgccgcatt
    10981 cagttttgtt aagtctgcca ctactgcagc atctttgcta tggtctgaag gaataccaaa
    11041 ttgcagagca gaaatttgat caataggtat agtcagattg ccatatgcag tagtaatcgc
    11101 tattttgttt aatgtagatt tgccggaaat aatatctccg tttttcaaat gaaccgtcag
    11161 cattacattg gctgcccaaa cactagaaat ggtgaggctt agtaaagaaa ggaaaaataa
    11221 ttttttcatg agatgaaatt tacgtggttt ttgaatgata actataaagg tgtaaaataa
    11281 gattcaccca actgcaaaat taatgctttt taacatgagc cgtttttatt acaagcgatt
    11341 actttttcaa atgtttaaat gctctgccta aattcttaat gatatcactg gcaatcaaac
    11401 tttcttcact ttgttttttg agtgccagat ctgcagctga gccatgtaaa aatactccta
    11461 aaattgagga ttcaagtgca ctgtaacctt gagccagtaa gccgcaaatg attccggtta
    11521 atacatcgcc actgccggcg gtagccatac ccggatttcc ggtggagttg aagtaaacat
    11581 ttcctgatac atctgcaata gcagtgtgcg caccttttaa tagggtaatg actttgtgtt
    11641 ttttagaaaa tgactgtaat ttttgcaatc tatcaaaatc atctgtccat gttcctgtca
    11701 gtctttcaaa ttctttagga tgtggcgtta agatgcagtt ttcaggcaat aacttaagca
    11761 ttgctttatg ctgagcgatg aggttgagcg catcagcatc aatgacgcaa ggagttttat
    11821 tagcttgcaa aaaagatttc aatgcagaag ctgtatcctg atgtgtacct aagcctggtc
    11881 ctatggctac tgcagcatat gtcatttgta tgggaatacc ggaaatgtgc atattgttat
    11941 catcacaaat acacatggct tccggtagtg ccgtctgcaa aattgaataa ccacatcccg
    12001 gaatatgagc agtcagtaag ccggctccgc ttcgcatgac ggctttagca cataatgtag
    12061 cagcacccat tttaccatgg cttccggcaa ctaataaagc atgtccgtaa gtgcctttat
    12121 ggctgaattt attacgggtt tttaggagcg cttttatctc cttcttattc gtgtaaaaat
    12181 aaggcgtatt gatttttgaa ataaaatcag gatgaagacc aatatcagca accttgaaat
    12241 tgccgactgt attcccgctt ggaggcaata agaaagaaag tttcggaaac tgaaatgtat
    12301 atgttttttg agcttgaatt ataggcattc cggattgctt ttcagtatac aagcctgacg
    12361 gaatatctac cgaaattata ctcgattctt gctcatttaa atagtttatc aatgcagctg
    12421 ataatccgtt tattggctta tttaagccgg agccataaag tgcatcaata acgatatcct
    12481 ctttatctag ctttggaaag tcattagcac tcaaaatgca tttataatgt tttagtcttt
    12541 gcaagtttag ttgaaagtcg gctgaatact gactactgtg aagtataaaa acgtttacag
    12601 catgtttttc atgaagtaac aagcgggcaa tggccagtcc gtcgcctcca ttatttccag
    12661 gaccacaaaa aatgtatttt gtttgttttt tatccggata atctttgagg aaaagagctg
    12721 tccatttttt agcagcacgt tccatcaaat caatacttgc tattggtaca ttttttatgg
    12781 tataagcatc cgcttctcga atttgatttg cacttaaaat tttcatattc aaaattaatc
    12841 agaattgcac agatgaagca tgaactatat cagtaaaata aaataatcat atataaaact
    12901 attgcgttaa cttcagcttt ccgtagactg gctgaaattt caatctaaaa atgactttaa
    12961 atatgatttt tagcacagtt agttaaaata ttgataaatg ttcaaaagaa atgctttttt
    13021 ccctaccagt tttaccctga tacataccga atacctgaaa aattgacccg aatactaaat
    13081 aatctcattt cattttgtaa ttgccttatt tttataccat cattttaagt tgtttacgtt
    13141 taaaataagt tcgtgctctt ggttagttta cactcataaa taaaaagaag tacgacaggg
    13201 tctaaaagct ctaaaaattt ttcgggatgc tatgcatccc gttttttttg ctattttgta
    13261 agaaggaatt taaggatgtc cggattgctc aaccatatgc gttttaattt tctatcactt
    13321 ttgctgctag cttatctgtc tggttttgcg ctggatgtat ccttgttcaa attcaaaaca
    13381 ctggatataa aagatggctt gtcgcaaaac agtataaact acatttttca aggaagcaaa
    13441 ggctatatgt ggttttgtac aaatgagggg ctgaacttgt ataacggtta tagctttgaa
    13501 gtctttaaga atcaaataga caacagcaac tctttggtgt caaattttac acagtgcatg
    13561 gcagaaacca gcgatggcgt attttggata ggcacagagc ggggcatcag tgtatttatg
    13621 ccggataaga aaatatttgt caataccgaa agtccattgc aaattaaacg cattattgct
    13681 gaagacgata aagcggtatg ggtgcaaacg aattacagtg ttttcagggc tgaattagtg
    13741 caaattggaa aaaaaggatt aagtcccaaa gattacgcca tcaatataaa attttattac
    13801 gattacgatg aaatactcta taaggataaa gagggtaaaa tattgctgag ttctaaaaat
    13861 gatttgctgt ataattatgt accgaaatca caaacagttg tattgcgcag ttcatcaaaa
    13921 atttgggcgg cactaagtgc aaaaaagata aatagtgtag tacaggataa tcagtttaat
    13981 tactggattg caacgaacag tggggttttt aaatgcgata aaaattttaa cgatatcgat
    14041 actttttctg ctaaccggaa aaaattaccg gacttgcaaa gtaaaattaa tgatttgagt
    14101 gttgataagg caggtaatat ttatatggct accagtcaga acggtttgat tatttatgat
    14161 gcaaagcaag ctaagtttta taattaccaa tacgatattt ttgatttgac ggggcttccg
    14221 gataataaac tgctcagtgt attggtagat aaaagccaga ctttatgggt aggcagcaag
    14281 ggagcaggaa tatcagctta ctctccgtat aagtataaat ttaaattgat cacacaagag
    14341 ccatttaaaa cttcctggct gactaataaa tatattcttg ctattgaaaa ggacaagaca
    14401 ggaaatatat gggtgggtac tgatggcgga ggattatttc gttatgatgt taaaaagaat
    14461 ttgtttacca actggcgaaa taattcagct ttcagttctt tatctaatga tgtggtgcaa
    14521 gatattttaa ttgataaaaa aggtacgcta tggattggta ctttaaacgg catctgtaaa
    14581 ttttcaacat caggaaataa cttcatacgt tattatttta ctgcgaataa tgcccatgaa
    14641 aatttaacag tcaatcgcta ttccaatatc cgtttatttg aaaagagcaa tggtgaactt
    14701 tacgcctttg acgataataa tatttataag tttaatgcta tccggcagga atttgatttg
    14761 ctcaatatca tgtattacag ccggcatttc attcctagat ttattacaga agatgatgac
    14821 ggcactttgt ggattgcatc taatgcgggt atctcccatc tgaatacggc ggaaggattt
    14881 attgatattg atgcattaaa aaaactgaat gaaaaatatt ttaaaaacgg cagggtatat
    14941 gccttgctaa aagaagatgc tgaccggttt tgggtagcta cgggcaataa tggtatttat
    15001 ctttttaata aaaaaacatt ttcgatagac gtcaattata cggaaaaatc aggattgagt
    15061 aataattatg tgtacagtat tttgaaagat aattcgggaa aattatggat gagtacaaat
    15121 cgtggaattt cagtttttga tcctatctct aaaacattta aaaattatga tgtgaacgat
    15181 ggcttgcaga gtaatgaatt taacggtggg gcatttttaa aaacagatga tgacgaaatc
    15241 tatttcggag gggtgaatgg ttttaatatt attcaggcta atgaagtgcc atttaatgct
    15301 tacaagccaa gcagtaatat tatttcggta aaagcaaatg atatacctgt tgatttcgac
    15361 cgttatagcc agcatccaaa gcgggtgaaa ttcagcaatg atcaggataa tatcgaattt
    15421 gaattttcgt cgagtgatta tgccaatccg cccaaaaata tgtttcaata taaactggaa
    15481 ggctatgata aaaactggac tacatccaac aggaattatg taaaatatgc caaactgcca
    15541 tacggaaact atacgctttt agtaaaggct tcgaataatg atgcggtatg gagcgacaac
    15601 atttccagat ttgaattcag gataaaacct atgttctggc aaaccattct gtttaaaata
    15661 ctctttgtca tcgcatcttt gtttttgtta tatcgtttta taaaaaatac catccgttcg
    15721 gcacgaatga aagaaataga aaaagcgcgt aaggaaaaac agaaaacgat ttatgaaaaa
    15781 caattagccg aaataaaatt aaaagcctta gtcgctcaga tgaacccgca ttttatcttc
    15841 aattgtatga attccattca agcaatgatt ctgtctgata aaaatatgga ggcctcaact
    15901 tatcttacta aattatccag actggttaga gccgtattgg agaattccgt aaaaacattt
    15961 attccattat ctgaaattat cgaaaatctg aaactgtatt tagaattaga atcactgcgt
    16021 tttgataaaa agttccatta tgatttaaag gttgagaatc tggatattta ttctgtagaa
    16081 atgccttcca tgctctttca gccttatgta gaaaatgcca tttggcatgg tctgttaaaa
    16141 aaagagggag aaaaaaatct gcaaatccat ttcttcaaac gagataatta ttgcatctgc
    16201 gaaattacgg ataatggtat cggcagaaca aaggcagctg aattaaacct taaaaaacaa
    16261 cataaatctc tcggcacggt aattaccaaa gatatgtttg aaacgcttca taaaatccgc
    16321 gacagcaaat atactgtaga tatcattgat ttatatgatg agcataacga agcaaccggc
    16381 acgaaagtaa aaattgaaat cgaaatcaat taagcattta tcaaaccttt agctttatct
    16441 acaattaact caatctgttt ttccaaagtc agatgcgtag tgtcgatgac aatagcatca
    16501 tcaacctgag ttaacggaga atcttcgcgg gtagaatcaa ttttatcgcg gtgaataagg
    16561 ttggatttta gttcttcaag tgaaaataat tttcctgttt ttgccaatag ctcatcttgt
    16621 ctccgcttcg tccgtacatc caagtcagcc gtcataaaaa acttgagttc agcatccggc
    16681 atcacaacac tgccgatatc c
//
LOCUS       DAOQII010000023        16627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1657_length_16627_cov_4.587678, whole genome shotgun sequence.
ACCESSION   DAOQII010000023 DAOQII010000000
VERSION     DAOQII010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1657_length_16627_cov_4.587678"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLF48_02205"
     CDS             <1..504
                     /locus_tag="PLF48_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48163.1"
                     /translation="YTIKLKETIFDEFSQTSTLNVAGIPIPAPDGLLAIPSQVKNAEE
                     APEFLKHYVYVDEVNEESNTARYGFKSLFADGLIKDLLQPGVPSIDIDKPMAEPGQKF
                     NASSMYFNLGLRFNLTSKKRQDKLYEKQGKTNYKKGSKKPKMTEPEPEPGTIIEEQPA
                     QDNTNQE"
     gene            complement(564..1385)
                     /locus_tag="PLF48_02210"
     CDS             complement(564..1385)
                     /locus_tag="PLF48_02210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF48164.1"
                     /translation="MQNKIKINLGRVQETLLLPLAARAKEAKYRHPILIDTKAIELMN
                     MLDVDRKKLLVNMTRIGIHGLAYRAKLMDDSIMQFLEKHPVGKILDIGCGLDTTFYRC
                     DNGKASWYDLDLPDSIALREQLLPIPDCRVKYIAKSMLDYSWMDDIGDISKGLFITIP
                     GVLPYFEEEDVKAFLVTIAQRLKGAEIMFDVISNLGKFFVDRRIKSAGMKQAHLEWAV
                     LKIEDVSNWSPHIEVVKAHNFFDNINTSTYPFYLRNAMRINQFMDMTQIFHLKFI"
     gene            complement(1480..2856)
                     /locus_tag="PLF48_02215"
     CDS             complement(1480..2856)
                     /locus_tag="PLF48_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF48165.1"
                     /translation="MAYAKASTLKTKILFACGQIGWCLAAYGVTDLVFQFYLPPDNGT
                     PLFKAFIPQTTFFGIFKLVGIITFIAYVISAFMEPIVATLSDNSHFKWGKRKTFMLFS
                     ALPLAILSVLTFFPPTPYISQLNAAWMSVCILAVFLIICFYTTPYNALINELSHSEAE
                     RMSITMIISVAYALGIGIGQTINLFIKHLGDTMGNERAFQTGILLFGILSAIFMYIPV
                     LFVPDNSNTEKSYENKNTPFLQMISDVWSNKNFRYFAFIELLNWYPQKIFTIAVPYIV
                     ATLMKLDNVYTSYILYSLGLFSFALYPLIAKLTARFGKRKMMISACAFLMLSYLYTTF
                     YGYFKLPTFLAVSLYILLNIYPTAVLGLLPMAMAGDMAQADAQKTGIYKNASYYGMKT
                     FMMKMGIALTSLIFPSLLLLGNTPGHTMGIRLVAFVSVITSLLSIILLMKYKDPVILM
                     SDKTMNDK"
     gene            complement(2871..3932)
                     /locus_tag="PLF48_02220"
     CDS             complement(2871..3932)
                     /locus_tag="PLF48_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNF48166.1"
                     /translation="MKNKNEILVGALTIIGLTLGILGFKFLQGDSMFTRSKYVYIVAD
                     RTNNIVTSNGVFENGVAIGRVDKIELSNSPNYSHKAIMRLKLNNNVFIPNNSKFIIYG
                     LDMLGKMGIGHLRGSSTKEATDADTLECMVKGNAIDQGVDLISQLKPKLDTLLDNFSG
                     LSANLNNQLGKGENGLLKKAVGDLSTTLVSVNRLAGNADKMISTLNVTISDNKGNLDG
                     ILKNVNQLTAKFNNETGKIDSILTNFEKLSGKLAALDLEATVTSARNTLEELKKTLKL
                     VNDGDGSISKLLKEDGAYNDITKTINSLNALLTDLKANPKKYISLSLIDRSKTYSVTD
                     TSVQQFLENNPKAAKKVGK"
     gene            complement(3976..5295)
                     /locus_tag="PLF48_02225"
     CDS             complement(3976..5295)
                     /locus_tag="PLF48_02225"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNF48167.1"
                     /translation="MLSLSLQSLAGAGAGNNYTMKTIVLDAGHGGHDTGCNGPSGSYE
                     KNVTLKVILALGKLIEDKYPNIKVLYTRKSDIFITLQDRAETANLNNADLFLSVHCNA
                     NPNKSAYGAETYLMGLHKTEANLDVAKRENAVIKLEKNYQKTYEGFDPDSPESMIALS
                     LAQNANIEQSTLLASKVQEKFTKDLNRYNRGVRQAGFWVLYRTTCPAILVETGFLTNS
                     NEEKYLISANGQQELAGAIFNAFEEYKNLLEKGSFTEPVLQTPTESSATTAIENSSTA
                     TLTNETKTDVVNTTSPVTISAPSTSTVKNNDNAANKKGKTVEPFDMDMNTPKTASKTK
                     IDKQKNTPPPPAKVIADNSGIQYKVQIRATDKPLPATDKCYKFFEDVSYENINGILKY
                     VLGPYPAILQAKSSLKKAKSSGYHDAFICVYKDGIRLSSTDASKYLR"
     gene            5375..8146
                     /locus_tag="PLF48_02230"
     CDS             5375..8146
                     /locus_tag="PLF48_02230"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNF48168.1"
                     /translation="MHRFFLHILFSVFLFYAGTVSSYAQTASQTDSAISEKFLKNDTE
                     EDSIQQDSLKFISKLQKDPADSLLKNSTNVEVKTIKIAENDLEDEVKYTAEDSIIYDI
                     PNKKVYLYGHANIKYQEMDMYGGFIQFDWDSSEVYAKATPDSLGYLKKIQFKDGTGEY
                     VAEAAKFNFKTKKGKSYGLVTKEMEGFLHGDQVKVVDSVTLYVKNARYTTCDLDDPHF
                     YIEVGKAKVVKDKYIVGKPANLVIEKVRTPLVLPFAFLPNIKSKGSGLLTPTYGQSTN
                     LGYFFQNVGYYWHINDKMDLTTTADIYTLGSWALRVNYNLKLLYKFYSTFNFTIAQQR
                     GSIEYERFYPNRKKAPFNFGIQMQMNIDPKRMYNSSFNANINITSSKTFQYLNSRDPQ
                     SVLSTTFTSNISYSKWWPGKPFQLTVSSSLNQNTQSKVIAVTVPSMHFSVTRINPFQS
                     KVQRTKRKWYENIGFSYDFNAQNTMSGIDSLFFNKENLKKNTNSITHSIPISGNFQLF
                     KYINFNVNFNYNEKWFFNYVDKTYYDEYVKYNIETKKYDTTYNSAISDIKFGFKTYRD
                     FNLGFSFGTNLYGLFQFKHSKLQAIHHRFSPSLNFNFAPDFTAPKWKYMRTVQSDSIG
                     NTEQYSIYSNNGVNPAQKQGTIGLRFSNTIQIKVKSKKDTVNGYKKISLLDALDFGLS
                     YNMFTKQLSPLNINGNTRISEYINLSFGVSMNPYSLDSLNRQTKTFYWKEAKRFFRFT
                     NAQITINGSIRSKKAGSVENQQLNQQLYQQQGFTNQLYSMSPYEKSFYNFTVPWSVNY
                     SYSFSVNKYKYNKKDTLTFNQGLSLGLDVNITSKWKLNVSSGFDFSSKKPKITRTDIS
                     VIRDLHCWQLEFRWTPFGYQAGYFISLYVTSQQFSWLRLQKQKAFFDSGFFGSGGVNS
                     NAFGNFLPK"
     gene            8158..8859
                     /locus_tag="PLF48_02235"
     CDS             8158..8859
                     /locus_tag="PLF48_02235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNF48169.1"
                     /translation="MKRFYTDLIISNTAYLQDVEAGHCTKVLRCRVGDSIECMDGNGL
                     LLKGTITEIKKHEVAVEIIETIEHQSSNINKLAIALPPTKNPARFEWFVEKATEIGIA
                     HIYPMLTERTEKASLKMERLNHIVISAAKQSRQLFYPKIYELKKMEDIMALNEAQQKL
                     IAHCVGQKESLINLYKKNEDALLLIGPEGDFTNKEIEQAINKNYQPVSLGNSILRVET
                     AGVAACAVVAAINAN"
     gene            8872..9534
                     /locus_tag="PLF48_02240"
     CDS             8872..9534
                     /locus_tag="PLF48_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNF48170.1"
                     /translation="MKYLSLVFFILINSIFVQSFAQGKSTAKIGLLKYKGGGDWYANP
                     TSLPNLVKFCNQNLNLNMNFQIDEVEPGSAELFNYSMIHLTGHGNIIFSAGEAENLRN
                     YLIAGGFLHISDNYGIDKFIRKEMKKVFPELEFVELPASHPIYHQKYDFSGLPKIHEH
                     DGKPAQGFGIIYEGRLVCFYDYECDLGDGWEDQQVHKDSENARQNALKMGANIVQYAF
                     TK"
     gene            9649..11265
                     /locus_tag="PLF48_02245"
     CDS             9649..11265
                     /locus_tag="PLF48_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF48171.1"
                     /translation="MHSLYQSYKQQPVIQDFYDAICIGSGLGSLTTASLLARKGWKVL
                     VLEKHTTPGGFTHVFKRNKYEWDVGLHYVGDMHRKGSMARLVFEHITDGNLKWADMGE
                     VYDHVIIGDKSYEYVKGLQNWKTKMKSYFPTDNDARAIDKYVELLFAVNKSSKSFFMQ
                     KALPDFIRFLTNPFLGNDYKKFASKTTQQTLDEISDNDELKAVLSAQFGDYGMPPDES
                     SFVIHAAVALHYMNGGYYPIGGSEEIFNTIAPSILENGGAIIVGADVQEIETANGIAT
                     GVKMRDGKTIKSKLVISGAGVHTTFNHLISATDKQKIKYDQDVKYLPHSSGHLSLYIG
                     LKETAADLKLPKANYWIYPNGNLHNIAVKDFLENYKKEFPVVYISFPSAKNPKFEEKY
                     PGRATIEIITVAKYEWFQKWENEKWKKRGEEYDRLKENFAQRLLEHLYRVEPQLRGKI
                     DYYELSTPITTRHFCNYGKGEIYGLDHGTIRFEKDYLQPKTPIKNFYLTGQDIVTVGI
                     AGALMSGVLTASAILKTNLISDMVKQAKGL"
     gene            11436..12212
                     /locus_tag="PLF48_02250"
     CDS             11436..12212
                     /locus_tag="PLF48_02250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF48172.1"
                     /translation="MDILNYNRKAWNHQVELKNKWTLPVNEQDIVDAQNGKYDLLLTP
                     KKPVPVNWLGELNGKQVLCLASGGGQQVPIFAALGAIVTSFDLSDAQLQQDKETCEKF
                     GLQVQLVQGDMADLSALQNDTFDLIFHPCSNCFSEEILPVWKECFRVLKKGGVLLSGF
                     CNPIIYAFDAEKRDEERVLEVKYKLPYSDRDSLDETQRNKFKEENEPFCYSHSLTTQL
                     GGQLKAGFVLQDLFEDNWGGEEAEDRYFDSFIATKSMKPH"
     gene            12256..14238
                     /gene="rnr"
                     /locus_tag="PLF48_02255"
     CDS             12256..14238
                     /gene="rnr"
                     /locus_tag="PLF48_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNF48173.1"
                     /translation="MKKRKINKKKDASRRTSKKSEEKLLIGDEMIGVIDIARSGDAYV
                     KVEGKNKDLFIHRKNTFKAFDGDTVKIMRMHKPNAIRQEAVVTDIVQRVRTQFIGVVS
                     RKNDTCFVIPDNSGIKVDFYIPENRSKGAKENDKVVVDFAEWRDKDKNPIGHITEILG
                     NAGTNDVEMKSLLVENGFYTAFPKEVLNATDELDMQISKQEIKKRRDFRNVPTFTIDP
                     QDAKDFDDALSIQKTADGLWEIGVHIADVSHYVAEGSILDKEAYKRATSVYLVDRVAP
                     MFPERLSNIICSLRPNEDKCCFAAVFKMNDKAEVKDVWYGRTVIHSQKRFTYEEAQEI
                     LEGKDGVFKEELTTLNKLAYTLRKIRFANGSINFEAPETKFKLDEKGKPVGVYVKERK
                     DAHLLIEDFMLLANKYVAYFVGKEKNANGKTPMVYRVHDLPDEEKLMDFSLFVRKFGH
                     KVIHFDNPKQLSRDLNRLMSDIQGKPEQNVLQQLAVRCMAKAVYTTNNIGHYGLGFEY
                     YTHFTSPIRRYPDVMVHRILQDVLDGKKMPGKDEVEMKCKHSSERERAAADAERASVK
                     YKMAEYMLDHIGEEFEGVISGVKNWGIYVELPEYNCEGLLRIEKLNDDVYKYDERYLK
                     FTGKHSGKTYQIGEPIRVKLSEVDLEKKTIDFDLVG"
     gene            14378..16393
                     /locus_tag="PLF48_02260"
     CDS             14378..16393
                     /locus_tag="PLF48_02260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNF48174.1"
                     /translation="MKHYLFTAVFFLLLSSAFAQSKDVVGANVDATVKPGDDFFKYAN
                     GGWIKKNPIPAAYSQWGIGNLVQEDVWDKLYAVNRSAFKKVEMGNADLSTKQIAGFFQ
                     SAMDTATINKLKLQPIAKELAMIDSIKDKKGLITVITALHKLGVATSFDIGIYQDAMN
                     SDVNALYLTQGGLGLPNRDYYFNKDARTQKIVKDYQTNHLPKMLGFVNPNYRKYSSTV
                     FSLEKNLASRSRKLEDLRDPYANYNKMSIAQLNKLTPQINWNTVFQQLDIKVDSVIVG
                     QPEFFKKLDSMINMMPLSVWKNYLRYHVLTGFADYLYKDVENEDFRFYGKVIRGRKEM
                     LPRWKRMLMWEESAMGELLGQLYVKDNFNAKTKERYEKLCDEVFSAFAARIKNLNWMS
                     DATKQKSLQKLSTVVKKVGYPDKWKDFSSLKITPNSLTENIKNVNEFWFKYQVSKLGK
                     PVDRTEWDMTPQTYNAYYNPSNNEIVLPAGIFVIPGFKDEEIDDAVIYGYAAASTIGH
                     EITHGFDDEGRQFDAKGNLQNWWQKEDEDKFNAAAQKYIDQFNNYVVLDSMHINGKAT
                     LGENIADLGGLVIALDAFKKTQQYKEGKLIDGYTPLQRYFMGYALGWLGHQRDEELAT
                     RVLTDVHSPAFLRVNGPFSNIDDFYKAFNILKGDKMYRDEKDRVKIW"
BASE COUNT         5489 a         2836 c         3235 g         5067 t
ORIGIN      
        1 tataccatta aactgaaaga aactattttt gatgaatttt ctcaaacatc aacattaaat
       61 gttgcaggca ttccaattcc ggcaccggat ggcttattgg ctattccttc gcaagtaaaa
      121 aatgcagaag aagcaccgga gtttctcaaa cactatgtgt atgtagatga agtaaacgaa
      181 gagtcaaata cagccagata cggttttaaa agtttgtttg ctgatggtct tataaaagat
      241 ttattacaac caggcgttcc tagtatagat atagataaac ctatggcaga gcccggacaa
      301 aagtttaatg catcctccat gtattttaat ttaggtctgc gatttaatct gacatctaaa
      361 aaacgtcagg ataaattata tgagaaacaa ggaaaaacaa actataaaaa aggaagtaaa
      421 aaacctaaaa tgacagagcc ggaaccagag ccgggtacta ttatcgaaga acaacctgcg
      481 caagataata ccaatcagga ataatattaa caaaaataaa tttaaaacag gctggtcatt
      541 agatcagcct gtttttttta tactcagata aatttaagat ggaaaatttg tgtcatgtcc
      601 ataaattggt taatacgcat ggcatttcta agataaaacg gataagtgga agtattgatg
      661 ttgtcaaaaa aattatgcgc tttaactact tcaatatgtg gcgaccaatt tgaaacatct
      721 tcgattttta aaacagccca ttctaaatga gcttgtttca taccggctga tttaatacgc
      781 ctgtcgacaa aaaatttgcc gaggttggag atgacatcaa acataatttc cgcacctttg
      841 agccgctggg caattgttac taaaaatgct tttacatctt cttcttcgaa ataaggcagt
      901 acgcccggaa tggtaataaa caatcctttg gaaatatcac cgatatcatc catccaggag
      961 taatcaagca tagactttgc aatatattta actctgcaat ctggtatagg cagtaactgc
     1021 tctcttagtg ctatgctgtc cggcaagtcc aaatcatacc atgaggcttt tccgttatca
     1081 catctgtaaa aagtggtatc taacccacac ccgatatcca gaattttacc cacgggatgc
     1141 ttttctaaaa attgcattat tgaatcgtcc atcaattttg cccgataagc caagccatgt
     1201 ataccgatgc gggtcatatt caccagtaat ttctttctgt ccacatctaa catattcatg
     1261 agctcaatag ctttcgtatc aattaatata ggatgcctgt atttagcctc tttagcgcgt
     1321 gcggccaagg gcaaaagaag tgtttcttgt accctgccta aattaatttt tatcttgttt
     1381 tgcatatata cgttctaagc gtcgatatca atgaaagaaa atgacaaaag atggagatgt
     1441 atttacagat tacaattaat aagcatcatt cattatttat catttatcat tcatcgtttt
     1501 atcactcatc aaaataaccg gatccttata cttcattaat aaaataatgg aaagcaggga
     1561 tgtaatgacg ctgacaaacg caaccaacct aatgcccata gtatgaccgg gtgtattgcc
     1621 taagagtaac aaggatggaa aaattaagct ggtaagggca atccccattt tcatcataaa
     1681 tgttttcatg ccataatagg atgcattctt atagatgcct gttttttgag catcagcctg
     1741 tgccatatca cccgccatag ccataggcaa caaacccagt acggcagtcg gataaatatt
     1801 gagcagtatg taaagactta ctgccaaaaa tgtaggtagt ttaaaatagc cgtaaaatgt
     1861 ggtatataaa taactcaaca tcaaaaatgc acaagctgaa atcatcatct ttcgtttccc
     1921 gaaacgcgca gtcagtttag ctattaacgg ataaagcgca aaagaaaata atcccaagct
     1981 atacagaata taggaggtat atacattgtc gagtttcatt agggtggcaa cgatataggg
     2041 tacggcaata gtaaatattt tttgaggata ccagttgagt agttcaataa atgcaaaata
     2101 tctgaaattt ttgttgctcc atacatcgct tatcatttgt agaaaaggcg tgtttttatt
     2161 ttcataggac ttttcagtgt tgctgttgtc gggaacaaac aaaacaggaa tgtacataaa
     2221 gattgccgac agtattccaa agagtagaat acccgtttga aatgcccgtt catttcccat
     2281 agtatctcca agatgtttga taaataaatt tatcgtttgc ccaataccaa tacccaatgc
     2341 gtaagcaaca gaaataatca tggtgattga catgcgttct gcttcgctgt ggctcagttc
     2401 attaatcaat gcattatacg gagtggtata aaaacaaatg attaaaaaaa cggccaggat
     2461 gcaaacagac atccatgcag catttagttg acttatatat ggagtaggag ggaagaaagt
     2521 cagtacactt aatatcgcta aaggcagtgc agaaaacaac ataaatgttt tgcgttttcc
     2581 ccacttgaaa tggctgttgt cgcttagtgt ggccactata ggttccataa atgcactgat
     2641 gacatacgca atgaatgtta tgatacctac cagcttaaat attccgaaaa aggtagtctg
     2701 aggtataaat gctttaaaga gcggcgtacc attgtcgggt ggtaaataaa attgaaaaac
     2761 gagatcggtt actccatatg ctgccagaca ccatcctatt tgaccgcatg caaacagtat
     2821 ctttgttttc agcgtggatg cttttgcata agccataaat aaaactaaaa ttacttacct
     2881 actttttttg ccgctttggg attgttctct aaaaattgct gtacggaagt atctgttacc
     2941 gaatatgttt tagaacggtc aattaaagac aagctgatat attttttcgg gttcgctttt
     3001 aaatcagtca gtaaagcatt cagcgagtta attgttttag tgatgtcatt gtaagctccg
     3061 tcttctttca gtaatttaga aatagagcca tcaccgtcgt ttactaattt caatgttttt
     3121 ttcaattctt caagcgtatt acgagcactg gttacggtag cttccaaatc cagtgcagcc
     3181 agtttgcctg aaagtttttc aaaattagtc aggatagaat caatttttcc ggtttcatta
     3241 ttaaacttgg ctgttaactg atttacattt ttcaaaatgc cgtctaaatt tcccttattg
     3301 tctgaaatgg ttacatttaa agtagaaatc attttatcag catttcctgc caatcggtta
     3361 accgaaacta aagtagtaga taaatcacct acagcctttt tcaataagcc attttcacct
     3421 ttgcctaatt gattattcag attagctgat aagcctgaaa agttatccag taaagtatct
     3481 aatttgggtt taagctgact aattaagtca actccctgat ctattgcgtt accttttacc
     3541 atgcactcta aagtatccgc atcagtggct tctttcgtgc tgcttcctct caaatgccca
     3601 atgcccattt tgcccagcat gtctaatccg taaatgataa atttactgtt attgggaata
     3661 aatacattat tattcagctt taagcgcata attgctttgt gagagtagtt gggactgttt
     3721 gataattcaa tcttgtctac acgaccaatg gcaactccgt tttcaaaaac tccgtttgaa
     3781 gttacaatgt tatttgtacg gtcggcaacg atatacacat atttactgcg tgtaaacata
     3841 ctgtcgccct gcaaaaattt aaaccccaag atccccaagg ttaatccgat aatggtgagt
     3901 gcgcctacta aaatttcgtt tttatttttc aatttctcag gtttaaactc accaaatata
     3961 aggagaaaat tgaaactatc tcaaatattt agaggcatct gttgaagaaa gtcggattcc
     4021 atctttataa acgcaaataa atgcatcatg atacccggaa gattttgcct tttttaagga
     4081 agatttagct tgtaaaatgg caggataggg tcctaaaaca tacttcaata tgccatttat
     4141 gttttcatag ctgacatcct caaaaaattt ataacattta tctgttgctg gaagtggctt
     4201 gtcggtagct cttatttgta ctttatattg tatgccactg ttgtcagcaa taacttttgc
     4261 aggaggtggc ggagtgtttt tttgcttatc gatttttgtt ttggatgcag tttttggggt
     4321 attcatatcc atatcaaacg gctcgactgt tttgcctttt ttgttagcag cattgtcatt
     4381 gttttttaca gtagatgtag aaggagccga aattgttacc ggactcgttg tattcacaac
     4441 atccgttttt gtttcatttg ttagtgtggc tgtagagctg ttttcaattg ctgttgtagc
     4501 tgagctttcc gtcggggttt gtaataccgg ttcagtaaat gaaccctttt ctaatagatt
     4561 tttgtattcc tcaaatgcat taaaaatagc cccggccagt tcttgctgtc catttgctga
     4621 tattaaatat ttttcttcat ttgaattggt caaaaagcct gtttcaacta aaatagccgg
     4681 gcaggtggtg cggtacaaaa cccaaaatcc ggcttgtcgc acaccccgat tgtatcggtt
     4741 taagtctttg gtaaattttt cctgtacttt actggctaat aaagtacttt gttcaatatt
     4801 ggcattttgc gccaaagaca gggctatcat agattcggga gagtcgggat cgaagccttc
     4861 atacgtcttt tggtaatttt tttcaagttt gattaccgca ttttctcgtt tggcaacatc
     4921 cagattggcc tctgttttgt gtaaacccat taaataagtt tctgctccat aagcactttt
     4981 attggggttg gcattgcagt gtacactcaa aaacaaatcg gcattattga gattagctgt
     5041 ttcagcacgg tcttgcaagg tgataaagat gtcacttttt cgggtataca gtactttgat
     5101 attcggatat ttatcttcaa ttaatttacc aagtgcaaga ataactttta aggttacgtt
     5161 tttttcataa gaacccgaag gaccattaca tccggtatca tgtccaccat gtcctgcatc
     5221 cagtacaatg gttttcatgg tgtaattatt gcctgcacct gcgcctgcca aactctgcaa
     5281 gctgagactt aacaaacaaa tgaaaaatgt ggtctttatt tttgtgatta taaacatatc
     5341 tttgaaactg tatttaattt gtaatattgt gctgttgcac cgtttttttt tacatattct
     5401 cttttctgtt ttcctctttt atgcgggtac tgtttcctca tatgcacaaa ctgcaagcca
     5461 aactgattct gccatatctg aaaaatttct taaaaatgat actgaagaag attcgataca
     5521 gcaggattca ttaaaattca tttcaaaatt acaaaaagac ccggcagatt ctttgcttaa
     5581 gaattccaca aatgttgaag tcaaaacgat aaagatagct gaaaatgact tagaagatga
     5641 agtgaaatac accgcagaag actccatcat ttatgatatc ccgaataaaa aagtgtattt
     5701 atatggtcat gccaatatca aataccaaga aatggatatg tatggcggct ttatccagtt
     5761 tgactgggac agcagcgaag tatatgcaaa ggctacaccc gacagtcttg gatatctaaa
     5821 aaagatacag tttaaagatg gcacgggaga atatgtggca gaagcagcaa aatttaattt
     5881 taaaacaaaa aaagggaaaa gctatggttt agtcactaag gaaatggaag gattcttgca
     5941 tggggatcag gtaaaagtag tagattctgt taccctttat gtaaaaaatg caagatatac
     6001 tacttgcgat ttggatgatc cgcattttta tatcgaggta ggtaaggcta aagtggtgaa
     6061 agataaatat atcgtaggca aaccggctaa tttggtaatt gaaaaagtgc gtacgccgtt
     6121 ggtattgcct tttgcttttt tgccaaatat taaatctaaa ggctctggtt tacttacacc
     6181 tacttatgga caatcgacta atttgggtta ctttttccag aatgttggtt attactggca
     6241 cataaatgat aagatggatt taactactac tgcagatatt tatactttgg gcagctgggc
     6301 attgcgggtt aattacaact taaaattatt gtataaattt tacagcacct ttaattttac
     6361 cattgcacaa caaagaggca gtattgagta tgaaagattt taccccaacc gtaaaaaagc
     6421 tccatttaat tttggtatac agatgcaaat gaatatcgac ccaaaacgca tgtataacag
     6481 ctctttcaat gcaaatataa atattactag cagtaagacg tttcaatatt taaactccag
     6541 agatccgcaa agtgtgctga gcaccacttt tacatcaaat atcagttaca gtaaatggtg
     6601 gccgggtaaa ccctttcagc tcactgtcag ttccagcctg aatcaaaata cacaatcaaa
     6661 agttattgca gttactgtac cttccatgca ttttagtgtt acccgtatta atccgtttca
     6721 gagtaaagta caacgaacca aaagaaaatg gtatgaaaat attggtttct cctatgattt
     6781 taatgcacag aatactatgt cgggaatcga ttctttgttc tttaacaaag aaaacttaaa
     6841 aaagaatacg aactctatta cacacagtat ccctatttcc ggaaacttcc agttgtttaa
     6901 atacatcaac tttaatgtca attttaatta caatgaaaaa tggtttttta actatgtaga
     6961 taaaacgtat tatgatgaat atgtgaaata taatattgaa actaaaaaat atgatacaac
     7021 ttataacagt gcaatatctg atattaagtt tggatttaaa acttatcgcg attttaattt
     7081 aggttttagt ttcggcacga atctatacgg tttatttcaa tttaaacaca gcaagttaca
     7141 ggccattcat cacagatttt ctccatcctt aaattttaat tttgcgcctg attttactgc
     7201 gccaaaatgg aaatacatgc gcacggtaca atcagactct ataggcaata cagaacaata
     7261 ttctatttac agtaataatg gagttaatcc ggctcagaaa caaggcacta tcggattgag
     7321 attcagtaat acgattcaga ttaaagtaaa atctaaaaaa gatacagtaa atggatacaa
     7381 gaaaatcagt ttgctggatg cacttgattt tggattgagt tataatatgt ttaccaaaca
     7441 actgtctccg ctaaacataa atggcaatac acgtatttca gaatatatca atttgtcttt
     7501 cggtgtatcg atgaatccat actcactaga ttcgctcaac aggcaaacca agacttttta
     7561 ttggaaagag gctaagcggt tttttcgttt taccaatgcg caaattacaa ttaacggcag
     7621 tatacgttct aagaaagctg gtagtgttga aaaccagcaa ctcaatcagc aattatacca
     7681 acaacagggg ttcaccaatc aactctattc aatgagtcct tatgaaaaaa gcttttacaa
     7741 ttttactgtg ccttggtcag tcaattacag ttattcattt agtgtaaata aatataaata
     7801 taacaagaaa gataccttga cgtttaatca gggtttatca ttgggtttag atgtcaatat
     7861 cacttctaaa tggaaactga atgtgagttc cggatttgat ttcagtagta agaagcctaa
     7921 aattacccgg acagatattt ctgtaattag ggatttgcat tgctggcaat tagagttcag
     7981 atggacaccg ttcggttatc aggcgggtta ttttatttcg ttgtatgtaa cttcacagca
     8041 attcagctgg ttgcgcttac agaaacaaaa agccttcttt gattcaggat tttttggttc
     8101 gggtggtgta aattcaaatg cttttggcaa ctttctccca aaataaaatt gacgagcatg
     8161 aaaagatttt atactgattt aattatatcc aatactgcct atttacaaga tgtggaagcc
     8221 ggtcattgta caaaagtttt gcgttgcaga gttggcgata gtatagaatg tatggatgga
     8281 aatggcttgt tgttaaaagg cactattaca gaaataaaaa aacatgaagt ggctgttgaa
     8341 attattgaaa caattgaaca ccagtcatcg aacattaata aattagccat tgctttgcca
     8401 ccaactaaaa acccggcaag gtttgaatgg tttgtcgaaa aagctacgga aattggcata
     8461 gcgcatattt atccgatgct gacagaaaga acagaaaaag catcgctgaa aatggaaaga
     8521 ttaaatcata ttgttatttc agcagcgaag caaagcagac aattgtttta tcctaaaata
     8581 tatgaactta aaaaaatgga agatataatg gcattaaacg aagcgcaaca aaaattgatt
     8641 gctcattgtg taggccaaaa agagagtctg attaatttgt ataagaaaaa tgaagatgca
     8701 ttattgctta ttggtcctga aggagatttt acaaataagg aaattgaaca ggctattaac
     8761 aaaaattatc agccggtttc attgggaaac agtatcttgc gtgtagaaac tgcaggtgta
     8821 gctgcatgtg cagttgtagc agcaattaat gcaaattaat ccattggaaa aatgaaatat
     8881 ttaagcttag ttttttttat cctcataaac agcatttttg tacagagttt tgcccaaggt
     8941 aaatcgacgg caaaaattgg gctcttgaaa tataaaggtg gcggcgactg gtatgccaac
     9001 cccacttcat tgcccaattt agtcaaattc tgcaatcaga atttaaacct gaatatgaat
     9061 ttccaaattg atgaagtaga gcccggcagt gctgaactct tcaactattc tatgatacat
     9121 cttactggac atggaaatat cattttctct gccggcgaag ccgaaaatct gcgaaactat
     9181 ctgattgccg gtggcttttt acatatttcc gataattacg gcatcgataa atttattcgc
     9241 aaagaaatga aaaaagtttt tccggagctt gaatttgtcg agttgcctgc atcccatccg
     9301 atttatcatc agaaatatga tttttcgggc ttgcccaaaa ttcacgaaca cgatggaaaa
     9361 ccggcacaag gatttggtat tatctacgaa gggcgtttag tttgttttta tgattatgaa
     9421 tgtgatctgg gagatggctg ggaagaccaa caagtacata aagactcgga aaatgcgcga
     9481 caaaatgccc ttaaaatggg tgctaatatc gtacaatacg catttaccaa ataaccaacc
     9541 tacgaggtcg tttaaaatag cctatttttt aagttattga aaatcatttg attagttttt
     9601 caacctacga ggtcaaaaat gtttattacc tttatttcat tctaaattat gcattcgctt
     9661 tatcaatctt ataaacagca gcctgtcatt caggattttt atgatgctat ttgtatcggt
     9721 tccgggttgg gcagtttaac aaccgcttca ctacttgccc gcaaaggctg gaaagtatta
     9781 gtgctagaaa aacataccac acccggagga tttacccacg tttttaaacg taacaaatat
     9841 gagtgggatg tgggtttgca ttatgtaggc gatatgcacc gcaaaggctc aatggcgcga
     9901 ttggtttttg aacatattac agatggcaac ttgaaatggg cagatatggg cgaagtatat
     9961 gaccatgtca ttatcggtga taaatcttat gaatatgtca agggattgca aaactggaaa
    10021 acgaagatga aatcttattt cccgactgac aatgatgcaa gagcaatcga taaatatgtt
    10081 gagttgttgt ttgcggtaaa caaatcgtcg aagtcttttt ttatgcaaaa agcattgccc
    10141 gattttattc gcttccttac taatcctttt ttgggaaatg actataaaaa atttgcttcc
    10201 aaaacaacac aacaaacgct tgacgaaatt agtgataatg atgaattgaa agcagtactc
    10261 tcagcacagt ttggcgatta cggcatgcca cctgatgaaa gcagttttgt tattcatgct
    10321 gcagtagcct tgcattatat gaatggtggt tattatccga ttggtggctc agaagaaatt
    10381 tttaatacga ttgctccgtc cattttggaa aatggaggcg caattatagt cggtgctgac
    10441 gtacaagaaa ttgaaacagc aaatgggata gcaaccggag tgaaaatgcg tgatggcaaa
    10501 acaataaaaa gtaaattagt gataagcggt gcaggagtgc ataccacctt caatcatctg
    10561 atttctgcta cagacaaaca aaaaatcaaa tacgatcaag atgtgaagta tttaccgcat
    10621 tcttccggtc atttaagctt gtatatcggt ctgaaagaaa ctgctgctga tttgaaattg
    10681 cctaaagcca attattggat ttatccaaat gggaatttgc ataatattgc cgtaaaagat
    10741 tttttagaaa attacaagaa agaatttccg gtagtttata tctcatttcc ttccgcaaaa
    10801 aacccaaaat ttgaagaaaa atatccggga cgagcaacta tcgaaataat tacggtggcc
    10861 aaatacgagt ggtttcaaaa atgggaaaat gaaaaatgga aaaaaagggg agaagaatat
    10921 gacagactaa aagaaaattt cgctcaacgc ttgctggaac atttatatcg cgtagagcct
    10981 cagttgagag gcaaaataga ttattacgaa ttatctacac ccataacaac acgtcatttt
    11041 tgtaattatg gaaaaggtga aatctatggc ttggatcatg gtaccatacg ttttgaaaaa
    11101 gactatctgc aacctaaaac ccccatcaaa aatttttacc tgaccggaca agatattgtt
    11161 accgtaggaa ttgctggcgc attgatgagt ggtgtattaa cggcttctgc aattttaaaa
    11221 accaacctca tcagcgatat ggtcaagcag gcaaaaggtt tataatttga ttttcatgtg
    11281 agtattttat ccatttcata acggttacaa ttatgctgtt cattcgtgtt ggggtggtat
    11341 gttgttttaa taacgtacat ttgtcaggga atttatgact aacaaattca aaatctttgt
    11401 ttatgtcaga ggattgcaaa gtaaaattgt aaactatgga tatactcaat tataacagaa
    11461 aagcgtggaa tcatcaagta gaattaaaaa ataaatggac tttacccgtt aatgagcaag
    11521 atatagttga tgcgcaaaac ggaaaatatg atttgctctt aacccctaaa aaaccggtgc
    11581 ctgtaaactg gctgggagag ctgaatggga aacaagtgct gtgtctggct tcagggggcg
    11641 gacagcaagt gccaattttc gctgctttag gcgctatcgt tacttctttt gatttgtcag
    11701 atgcgcagtt gcaacaagat aaagaaacat gcgaaaaatt tggattgcag gtgcaattag
    11761 ttcaaggaga tatggctgat ttatccgcat tgcaaaatga cacttttgat ttgatatttc
    11821 atccttgttc caattgtttt agcgaagaaa tattgccggt atggaaggag tgctttcgcg
    11881 tgctgaaaaa agggggtgtg ctgcttagcg gtttttgcaa tccgattatc tacgcattcg
    11941 atgcagaaaa aagagatgaa gaacgtgtat tagaagtgaa atacaagttg ccttattccg
    12001 atcgtgattc gttggatgaa acacaacgca acaaattcaa agaagagaat gaaccatttt
    12061 gttacagcca ctctctgact acgcaattgg gtggacagct gaaagcggga tttgtattgc
    12121 aagacctgtt tgaagataat tggggcggag aagaagcgga agaccgttat ttcgattcat
    12181 ttatcgctac caaatcgatg aagcctcatt aatgcgataa ttaatgaaat tatataaact
    12241 tattagaatt aaaaaatgaa aaaaagaaaa ataaataaaa agaaagatgc atcacgaaga
    12301 acttcaaaaa aatcagaaga aaaattgttg attggtgatg aaatgattgg tgtgatagat
    12361 attgcacgaa gtggggatgc atatgtaaaa gtagaaggga aaaataaaga cttatttatt
    12421 catcgaaaaa atacatttaa agcatttgat ggtgatacgg taaaaattat gcgcatgcac
    12481 aaaccgaatg ccatccggca agaggcagta gtaacagata ttgttcaacg tgtacgtaca
    12541 cagtttatcg gcgtggtttc acgaaaaaat gatacctgct ttgtcatacc cgataattca
    12601 ggaataaagg tagactttta tattcctgaa aatcgctcta aaggtgcgaa agaaaatgat
    12661 aaggtggtag tagattttgc tgaatggcgc gataaggaca aaaatcctat tggacatatc
    12721 acggaaattt tgggcaatgc cggaactaac gatgtagaga tgaaatcgct cttggtagaa
    12781 aacggatttt atacggcatt tcccaaagaa gtgctcaatg ctaccgatga gttggatatg
    12841 caaatctcta aacaggaaat aaaaaaacgc agagatttcc gcaatgtgcc cacttttact
    12901 atcgacccac aggatgctaa agattttgac gatgcacttt ccatccaaaa aacagctgat
    12961 ggtttatggg aaatcggcgt gcatatagcc gatgtttcgc attatgttgc tgaaggctca
    13021 attctggata aagaagctta taaacgagcc acttctgttt atctggtaga ccgcgttgca
    13081 cccatgtttc ctgaacgctt atccaatatc atttgctccc tgcgaccgaa tgaagataaa
    13141 tgttgttttg ccgctgtgtt taagatgaat gataaagctg aggtgaaaga cgtgtggtat
    13201 ggcagaacag ttattcattc gcaaaaaaga tttacttatg aagaagcaca ggaaattctg
    13261 gaaggcaaag acggtgtatt taaagaggaa ttaacgacct tgaataagct tgcatatact
    13321 ttacgtaaaa ttcgatttgc aaatggctct atcaattttg aagcaccgga aacgaagttc
    13381 aaattagatg agaaaggaaa gcccgtggga gtgtatgtaa aagagcggaa agatgcgcac
    13441 ttgctgattg aagattttat gttgttggcg aataaatatg tagcatattt tgtaggaaag
    13501 gaaaaaaatg cgaatggcaa aactccaatg gtttaccgtg ttcacgactt gccggatgaa
    13561 gaaaaattga tggattttag cttgtttgtg cgcaaattcg gtcataaggt aatccatttc
    13621 gataatccca agcaactctc cagagatttg aatcgcttaa tgtccgacat acaaggcaaa
    13681 ccggaacaaa atgtgttaca gcaacttgca gtacgttgta tggcaaaagc cgtgtatact
    13741 accaataata ttggacacta tggtttggga tttgaatatt acacacattt tacttcgcct
    13801 attcgacgct atccggatgt catggtgcat cgtattttgc aagatgtgct ggatggcaag
    13861 aaaatgcctg gaaaagatga agtggaaatg aaatgtaagc acagttcaga acgtgaacgc
    13921 gcagccgctg acgcagagcg ggcatcagta aaatacaaaa tggcagagta tatgcttgat
    13981 catatcggcg aagaatttga gggcgtgatt tcaggtgtga aaaattgggg aatttatgtt
    14041 gagttgcctg agtataactg tgaaggatta ttgcgaatag agaaattaaa tgatgatgtc
    14101 tataaatacg acgaacgcta tctgaaattt accggaaaac acagcggtaa aacctatcag
    14161 attggcgaac ccatacgggt taagctgtca gaagttgacc ttgaaaagaa aaccatcgat
    14221 tttgacctcg taggttgaaa tcttaaaatc aatatttaca gggcttccgg ctctttgatt
    14281 atgcccgaca acctcgtagg ttgaaaaaaa atggcttaaa agttgtaata ttatatcctg
    14341 aaataaactt atagaattaa aaaaaagtgc ttgaattatg aaacattatt tatttaccgc
    14401 tgtcttcttt ttattgttgt catcagcatt tgcacaatct aaagatgtag taggtgctaa
    14461 cgtggatgca accgtaaagc ccggtgatga tttttttaaa tatgccaatg gcggatggat
    14521 taagaaaaat cccattccgg cagcctattc tcaatgggga attggcaatt tggtgcaaga
    14581 agatgtatgg gataaattat atgccgtaaa ccgttctgct ttcaaaaaag tggaaatggg
    14641 taatgccgat ttgagtacaa aacaaatagc aggttttttc cagtctgcca tggacaccgc
    14701 cacaattaat aagctgaaat tgcaaccgat agccaaagaa ttggcaatga ttgattctat
    14761 caaagacaaa aaaggattga taactgtaat tacagccttg cataagttag gtgttgcaac
    14821 ttcttttgat attggcatat atcaggatgc gatgaacagt gatgtaaatg cactttatct
    14881 tacacaaggc ggtttgggat tacccaaccg cgattattat tttaataaag atgcccgtac
    14941 gcaaaaaatt gtaaaagact atcaaactaa ccatttgcca aaaatgctgg gattcgtcaa
    15001 tccgaattat cgtaaatata gttcgaccgt tttcagtttg gaaaaaaatc tggcatccag
    15061 atcaagaaaa ttggaagatt tgcgcgaccc ttatgccaat tataataaaa tgtcaattgc
    15121 acaattgaat aaacttaccc cgcaaataaa ctggaatact gtgtttcagc aacttgatat
    15181 caaagtggat tctgttattg ttggtcaacc ggaatttttc aaaaagttag attctatgat
    15241 caatatgatg ccattgagtg tttggaaaaa ttatttgcgc tatcatgtac tgacaggttt
    15301 tgccgattat ttatataagg atgtggagaa tgaagatttt cgtttttatg gcaaagttat
    15361 ccgaggcaga aaagaaatgt tgcctcgatg gaaacgtatg ctgatgtggg aagaaagtgc
    15421 tatgggcgaa ctgttgggac agctttatgt aaaagataat tttaacgcaa aaactaaaga
    15481 acgctacgaa aaattatgtg atgaggtatt ttcagcattt gctgcccgaa tcaaaaatct
    15541 gaactggatg tcggacgcta ctaaacaaaa atcattgcaa aaattgagta cagtggtaaa
    15601 aaaagtgggc tatccggata agtggaaaga tttttcttca ctcaaaatta cacccaattc
    15661 tttaactgaa aatattaaga atgtaaacga attttggttc aaatatcaag tgagcaaatt
    15721 gggcaagccc gtggacagaa ctgaatggga catgacacca caaacctata atgcctacta
    15781 taatcccagc aataatgaaa tcgttttgcc ggcaggtatt tttgtaatcc ccgggtttaa
    15841 agatgaagaa attgacgatg ccgtaattta tggctatgcc gctgcttcta ctatcggtca
    15901 tgaaataaca catggctttg atgatgaagg tcgtcagttt gatgctaaag gcaatttgca
    15961 gaactggtgg caaaaagaag atgaagataa atttaatgca gccgcacaaa aatatattga
    16021 ccaattcaat aactatgttg tactcgattc tatgcatatc aatggaaaag ccactcttgg
    16081 agaaaatata gcagatttgg gtggtttggt tattgcctta gatgcattca aaaaaacaca
    16141 acaatacaaa gaaggaaaat tgattgatgg ctatactcca ttacagcgtt atttcatggg
    16201 ctatgctttg ggctggctgg gacatcagcg tgatgaagaa ctagctacca gagttttgac
    16261 ggatgtacat tcaccggctt tcttacgtgt aaacggaccg ttttctaata ttgatgattt
    16321 ttacaaagcc tttaatatcc tgaaagggga taagatgtac cgtgatgaaa aagacagggt
    16381 gaagatttgg taattggaga ttagtgattg gttattggag attggttatt ggagattggt
    16441 cattaggcat tggtcattgg tgattagtta ttagttatta tttattagtt ctcaatactc
    16501 attactcatt actcaatact caatactcat tactcattac tcattactca atactcatta
    16561 ctcattactc attactcaat actcaatact caatactcaa tactcaatac tcaatactca
    16621 atactca
//
LOCUS       DAOQII010000024        16152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1715_length_16152_cov_6.196310, whole genome shotgun sequence.
ACCESSION   DAOQII010000024 DAOQII010000000
VERSION     DAOQII010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16152
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_16152_cov_6.196310"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2142)
                     /locus_tag="PLF48_02265"
     CDS             complement(<1..2142)
                     /locus_tag="PLF48_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48175.1"
                     /translation="MRWFIFILFLLKYTLSLFAGPITINNIDIVHPTCVNNGSMNINA
                     TEGAPAIGLLYSINGPVNASNSTGIFNSLPAGNYYVKVYNIGIDSAIQNVILTTTYVV
                     PVIQTVDTMRPYCDDDYTGSITGVIQSGTGFGPFQWRLDTITGATIRPFQSSATFSGL
                     PLGSYNLILQDCANTISYTVVFAATGYEFRGGLYGNGFPTAEKMHCDTFMIRFGINHN
                     LIEYKPPYRLRIATPNGNYWYNHSDITFMQDVGNVWFVSQKVGNIDYGDAISVAFYNA
                     CDDSITFNRTIARVEDMFYYFNLQCIGNVSIQANYYDSRPNLMITGLFAPVRFRLIDA
                     ATTATVFDETRTRTQSQILFEQFGYESILGVTMNNIPNNATYYFIMEDACGLIFRDTI
                     VLNAVQQPMSINTYISYPNGCLDSVIGAIHVQLYGFNAPTLTVLSGPPTLQSTNPMYA
                     YNDVNIYPKSFASVSDLVGGNYLGIWNVAAGTYHVRITDICGRQLDTFFVVNASSITG
                     LYHNFNYTPGCGNNNTINFTLGNSYTDNVTIRRVGGSNIFSGAMYANSSPLTVNIPNQ
                     PAGTYVVEYYLNGAPSSVTINSNPTCSFIRDTLIIGTQSTPQIQSYNVALCNADYIVE
                     IIPRTSVGVPPFEYQLEDAMGNVTGFQNEVFFTVAGVGNYTATIRDDCGNLSNYNVSV
                     NDIDYSLINFSGTYCLGDSARIQLVYSPYIRY"
     gene            complement(2225..2653)
                     /locus_tag="PLF48_02270"
     CDS             complement(2225..2653)
                     /locus_tag="PLF48_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48176.1"
                     /translation="MKTQITKLALIIMIIVSAVACKKNEVTPVNSALTADFDLVEDTV
                     QVTMPIISISIEEARFNITFNMINKSTSATNFLWKFSSGDLTSTDQNPSFTFHYLSEF
                     EPIMPGLYRKEVKLIASNSNTSRSAKKFIYVRVVSYPHSL"
     gene            complement(2925..3353)
                     /locus_tag="PLF48_02275"
     CDS             complement(2925..3353)
                     /locus_tag="PLF48_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48177.1"
                     /translation="MKIKILLLTLVFGFLIIQVRCTKEKIVEKIVYRDSIIYQDTFSH
                     RMYLLEFVAIPQTSTKSRVILNLKDLDEAVNTSYDNKLLNVNTAAIHATNTLTVSKAV
                     YDSVNYTDYYRSTTISRPSNDTLNVIFRIGTQGDVDIKTE"
     gene            3428..4813
                     /locus_tag="PLF48_02280"
     CDS             3428..4813
                     /locus_tag="PLF48_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48178.1"
                     /translation="MYQSKLFDIISAFHQAKINNFNHYLNIYRQKKENAVIRLFDYIF
                     EQFPSLDSPKLAKEQVHGVLYPDKEYDEKRMLNTMSDLLRLAEEFISYQASRENKLEN
                     SLHLLQFYLENDLNKQFESTFKETKLSIEQMPENTASLIISYRMELLHVEYQLKYDKR
                     NCDYHDCRIALNNLVVSQQKKLDNLYNINLYNDHDSTLVSSKLAQMQDMLHYLLLNDN
                     NTELYSEAKTLLTKEAKRFTKDELLFMVIIFINHCIRKVNEKIESYNQELLYWYDFLI
                     TYNCILEVNDTISSATVKNYITISLRLNQLQKADDFLEKYASVLSETEKEDVYNYNKA
                     NILFHQFNYEESLLLLSTIKYKDIFYKLSAKRLYIKIYYALTITNEKRYSDVMDSSLN
                     AFKKYIYTTKEITESIRTRNKSFYKYSAKLANLHKGEQKKLEQLHKEITQDTECADRD
                     WLLEKISILLH"
     gene            complement(4810..5553)
                     /locus_tag="PLF48_02285"
     CDS             complement(4810..5553)
                     /locus_tag="PLF48_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48179.1"
                     /translation="MQTKDEHIINKFREMISKRYDYEDLKKRFDVPDTITPEIINSIK
                     NYFLTVIYPPAAERKQLEEAFKNLGSYVRSPSKIWGLFGDMARAIFKFGRHFMAALRA
                     GMDSLNSFVGAKNFEASMAAIANKNGIQPPMTDQDFEDTLYQLPREEVEKFIGDVRHL
                     FGAMINTDLLKKTLDILDHVIQTMEKKSQTFPKEDVDGIRLGRGLLQKGYDLFSKMDE
                     KTKQDAVNLIYKNEMWFIDEVYKKKEGNV"
     gene            complement(5574..7331)
                     /gene="ppk1"
                     /locus_tag="PLF48_02290"
     CDS             complement(5574..7331)
                     /gene="ppk1"
                     /locus_tag="PLF48_02290"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNF48180.1"
                     /translation="MPNNPHKELIRILQPEELDAKQIAFVRNYFSEHVRAEIFPVMLD
                     KSTQTLQLKDTAIYLAVALKKNKRISAQAILEIPTFRLSRYLTLPSLYGELCVLFLDD
                     VIRLCLPEIFKVYSFDSISAFTLKYTKDTESEIDNELSVTFPDAAEKCVEKRKKNTSQ
                     FLIYEQAIPKPLLSLLIKKTNVTKGVQFVAVDRVHNFDNFIIPPFFNSSSTHHPIQEH
                     LNHPDFENNKSILQKMKKKDILLNFPFHSFIYVLDLLREAAIDPVVSHIRIILFRVAP
                     DSLVIRSLMTALQNGKDVTVFIELRARYDEEANIVWANKLRKAGARVITRVAGMKVHS
                     KLCLITRREKGQKIYYGCIGTGNLHEKTSKLYNDVMLLTANQNITKEAREIFAFFDKK
                     STDLKPKHLLIAPFNLREQLYSLIQQEIQHIKAKKPAEIFLKINNLADKGIQQKLIQA
                     AKAGVKIKLIVRGICGFVNYAEKELKNIKIISVADEFIEHGRIFIFSNGGKSKYYIGS
                     ADLMTRNIDYRLEVLTPVYDKTLQKQLYQTMSILWKENKNQQTIHLTPTLLKNGITHS
                     GLIEYWKHNAQKKLPNLQP"
     gene            complement(7350..7844)
                     /locus_tag="PLF48_02295"
     CDS             complement(7350..7844)
                     /locus_tag="PLF48_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNF48181.1"
                     /translation="MKTLTLIRHAKSCWENANLHDMVRPLNGRGEMSIPLIGNHLKEN
                     NIHPDLIYTSPATRALHTAIGIGGILGCHPEDFQINQEIYFGDATSIIELIKSTNNIY
                     RDVFLFGHDNFLADIVFELTDERPAKFPTCAAYRMQFETDNWQEIKNGKKIMFVYPKQ
                     FVKV"
     gene            7888..9399
                     /locus_tag="PLF48_02300"
     CDS             7888..9399
                     /locus_tag="PLF48_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48182.1"
                     /translation="MNGKNDIFELIHALSSTEKSYFKKFAYKIKTKDSKDYLALFDAL
                     VSQKEYDELLLKKKLKHTKLFNNFSASKTYLINLIIDTLSPYQEENNIHGIILKSLSQ
                     IRFLNDKKAVQLAGKKIQQVKKQIIENELYTYSHTLFQLEIDLIPYSKTEEKYRNQLF
                     LDCQYAERCVQNYSEYLQLKEEWIYFRQNTQTYTRDNSLQNTINEKLKHPLLQSADTA
                     LTTRAKYLYYYIRIGYYLLMNDLEAAYTDSCNQYVLLDKNLSFRQLYATDYINNLGNH
                     LFRMINVNQYEDANDVIQKLESALKNYPDKDFVQLKRLNILYIERARSMHEKDFPRML
                     QNIEFMENAIGYFKIRKDIAISLQYDIILAYFYNDKFADALMHANTLLNHEELNSFKD
                     LESYAHIIRLLCFIELNKKFVSDNLFDNLKKKLYRDKKLFKMENCMLRFLEEYANSTE
                     SKQKLAVLIQYKKEIEQLVQNPLEQNMLHYFNILWWMEAKINQKTMLSYFTKT"
     gene            9872..10723
                     /locus_tag="PLF48_02305"
     CDS             9872..10723
                     /locus_tag="PLF48_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF48183.1"
                     /translation="MHKKFALIGIAGYIAPRHLNAIKDIGGELVAAYDIADSVGILDA
                     YFPKCEFFTDEFAFYDFLIDCIPVDYLVVCSPNYLHEQHCYAGLKLNADIICEKPTCL
                     TLESWQNLADAQQYSTKKIFTILQLRHHPEIVALKNSIQTDLSYDVEIKYHTPRGKWY
                     HKSWKGDMKKSGGIATNIGIHLFDMVCWLFGEVLAFQVEENTETTSKGLLQFKNARVK
                     WDLNIGIEEKPVRSIAINAEQIEFTAGFADLHTASYHQIMDGKGYGLDDVKASIALVE
                     KIRTTAL"
     gene            complement(10727..11866)
                     /gene="bshA"
                     /locus_tag="PLF48_02310"
     CDS             complement(10727..11866)
                     /gene="bshA"
                     /locus_tag="PLF48_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNF48184.1"
                     /translation="MNIGIVCYPTFGGSGVVATELGKGLADKGHKVHFITYKQPARLL
                     DSFNANIFYHEVSLNDYPLFDYAPYETALASKLVDVAIYANLDIFHVHYAIPHASAAF
                     MARKILQTKGIDVPVITTLHGTDITLVGKDPTYEPVVTYSINNSCGVTAVSESLKIDT
                     YKHFDINKEIEVIPNFIDFSRFKKTNKEHFKKAIAPNGEKIIVHTSNFRKVKRVEDVI
                     YVFCKIAEALPAKLLLIGDGPERHNMEVLVRSLCDSLDVRFLGKQDAVEELLAVADLF
                     LMPSESESFGLAALEAMACEVPVISSDAGGIPEVNIEGVTGYLCPVGDIDCMATKAIK
                     LLKDERMLEQFRKNALAQAERFDIHNILPQYEYFYQKVKEKGDCV"
     gene            complement(11981..13363)
                     /locus_tag="PLF48_02315"
     CDS             complement(11981..13363)
                     /locus_tag="PLF48_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48185.1"
                     /translation="MNTRLVLWGEIGTDRKALIALYLDEPTTKIHIYAFPKEEVSKEI
                     QDQLFVEWKNGGEFAFPETAHHWEIDANSDTILPEEVKPERVDLVLQSQHKWSKKLMS
                     SKINQMLADETQLLEEQANVISDYDKSLWDKTKAQWEKIASYQKKNEITWEQTTVLKE
                     KINGIFDALKAMKRLNTENEDQANSVFTKQIQKRIEDLQSKLIYSDQWKYIFDELKKI
                     QHEIKDVTMRWSVKKNIYATINQIFDDLRKYRSTETVTKTQSRIVQLKQILDGLKDSI
                     ARDTDNLNMQIEKMQHYTRGKLGVDEIKNRFNYITDRIKDKEKKADGIKDTIKQLEKE
                     IEKEKQQQTEREEQRKKREEEQAQKEHTEKADAKSNDHADESKEVKTAVADKEVQATT
                     ATEENHAVTENMTSTEANATTITDAEHSDIPTETETTQSEDSVLNNELTENNAEEVKT
                     TEEAVAPENN"
     gene            complement(13502..14563)
                     /gene="rlmN"
                     /locus_tag="PLF48_02320"
     CDS             complement(13502..14563)
                     /gene="rlmN"
                     /locus_tag="PLF48_02320"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNF48186.1"
                     /translation="MKKDIRTFSKDELIAELEQLGEKPFRARQIYEWLWQKSAIDFHA
                     MTNLSKDLRQKLQQHFVIHPIQIFTTQHSNDGTVKTAFQLYDKHLIEGVLIPTDDRAT
                     ACVSSQVGCSLTCSFCATGFLKRERNLTAAEIYDQVVLINKQALETQGRNLSNIVFMG
                     MGEPLLNFNEVMDAIAKITSPEGLNFSSKRITVSTAGIAKMIRKMADDEVRFNLALSL
                     HAADDEKRNSIMPINEQNNIEALLDALVYFQKKCKGEVTFEYILLNGVNDSMEDAKKL
                     VALCHRLQGVKVNVIEYNRIEQAEFQKSDAIHRNHFIGYLTQNNVIANVRKSRGKDID
                     AACGQLANKNGNHHISTKK"
     gene            complement(14632..15624)
                     /gene="ppk2"
                     /locus_tag="PLF48_02325"
     CDS             complement(14632..15624)
                     /gene="ppk2"
                     /locus_tag="PLF48_02325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNF48187.1"
                     /translation="MAVKKTTTASSTKKAATSNGQVKPVKTAVKPAESKAEDPTKPEN
                     EWENKTVTLGELKNLKTKSDLSKLFHNNNGKPDKVLNSIKYEEELEKLQIELVKLQRW
                     IQEKGAKVAILFEGRDAAGKGGTIRRFTEHINPRTMRVVALPKPTEEERGQWYFQRYV
                     KQLPNRGELVFFDRSWYNRAVVEPVNKFCTQQQYEIFMQQVNEFEHMLYEDGVILIKF
                     WFSISKEEQLRRFESRKRNPLKQWKLSPVDMEAQKQWDVYTKFKEEMFSRTHTSFSPW
                     IIVRANNKQKARLETIRYVLSIIPYDGKDEATTRLHPDPNVISRYHRKILQVDK"
     gene            15752..>16152
                     /locus_tag="PLF48_02330"
     CDS             15752..>16152
                     /locus_tag="PLF48_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF48188.1"
                     /translation="MLRKLTADMIYPVSGQPIENGVLIIDHNGKIITLKNRDEFAVEE
                     LETYSGYIVPGFVNAHCHLELSHLKGIAQTGTGLIDFVTQVIANRNHSPEIIQQEIER
                     AENEMLQNGIVAVGDICNTTDTFYQKQKENL"
BASE COUNT         4962 a         2917 c         2892 g         5381 t
ORIGIN      
        1 gtatcgtata tacggcgaat aaactaattg tattctggca gaatcgccta aacaataagt
       61 cccactaaag tttatcaaac tataatcaat gtcatttact gatacattat agttggataa
      121 attaccacag tcatctctaa tggtagcagt ataatttccg acacctgcaa ctgtaaaaaa
      181 cacttcgttt tgaaaaccgg ttacatttcc catggcatct tccagttgat actcaaacgg
      241 tggaactcca acactcgttc ttggaattat ctctactata taatcagcat tgcataacgc
      301 cacattataa gattgtattt gaggtgtaga ttgagtacca ataataagcg tatctctgat
      361 aaaactacaa gtaggattac tgttaattgt aaccgaagag ggtgcaccat ttagataata
      421 ttctactaca taagtgccag ctggctgatt gggtatattc actgtcaatg gagaagaatt
      481 tgcatacatt gcacctgaaa aaatattgct tccgccaact cttcgtatag ttacattatc
      541 agtgtaagag ttgccaagtg taaaatttat cgtattatta ttaccacaac ctggtgtata
      601 attaaaatta tgatataaac cggtgatact actggcgttt acgacaaaga aagtatctaa
      661 ttgcctgcca caaatatctg taatacgaac atgatatgta ccggcagcga cattccatat
      721 acctaaatag tttccaccca ctaaatcact aactgatgca aatgattttg gataaatatt
      781 tacatcatta tatgcataca ttgggttggt gctttgcaat gtaggcggac ctgacaatac
      841 cgttaatgtg ggcgcattaa agccataaag ttgtacatga atagctccaa ttaccgagtc
      901 taaacaacca ttcggataag aaatataggt attaatactc atgggctgct gtacggcatt
      961 caagacgatg gtatctctga aaattaaacc acaggcatct tccattataa aataataggt
     1021 ggcattattg ggtatgttat tcatggttac gcctaaaata gattcgtatc caaattgttc
     1081 aaatagtatt tgactttgcg ttctggttct ggtttcatca aaaacagtag ctgtagttgc
     1141 tgcatctatc aatctaaaac gaaccggtgc aaacaaaccg gtaatcatca aattgggtct
     1201 ggaatcgtaa taattagctt gtatactaac attaccaata cattgcaaat taaaataata
     1261 aaacatatct tctacacgtg caatggtacg attaaatgta attgaatcgt cgcaagcatt
     1321 gtaaaaagcc acactaatgg catcaccgta atctatgttg cccacttttt gactaacaaa
     1381 ccaaacattt cctacatctt gcataaaagt tatatctgag tgattgtacc aatagttacc
     1441 attcggtgta gcaatacgca atcgataagg tggtttgtat tcaatcaaat tatggttgat
     1501 accgaagcga atcataaagg tatcgcaatg cattttttct gcagttggaa aaccgttgcc
     1561 gtataaacca cctctgaatt cgtagccggt agcagcaaac acaacagtat agcttatcgt
     1621 gttagcacaa tcttgtaata tcaaattata acttccaagt ggtaacccgc taaatgttgc
     1681 actgctttgg aatggtcgaa tggtggcacc ggtaatggta tctaaacgcc attgaaacgg
     1741 accaaaaccg gttccgcttt gtataacacc tgtaatagaa cccgtataat catcatcaca
     1801 ataaggtctc atggtatcca ctgtttgaat gactggcact acataggttg tagtaagtat
     1861 tacgttttga atagcactat ctattccaat attatacact ttaacatagt aatttcctgc
     1921 aggcaaacta ttaaatattc cggtagaatt gctcgcattt acaggaccat tgatagaata
     1981 taacaaaccg attgccggtg ctccttctgt ggcattaata ttcatagagc cattgtttac
     2041 acaggtggga tgtacgatat caatattgtt gatggtaatt ggaccagcga acaggctaag
     2101 tgtatacttt agcagaaaca gaataaaaat aaaccagcgc ataaatcaag ttttaataat
     2161 taagcctaag atattgtaat atgaatgaaa caataaatta tatgtttttt gcttcaaagg
     2221 tgtactataa gctgtgagga taagatacta ctctcacata gataaatttt ttggcagatc
     2281 tggatgtatt ggaatttgat gcaatgagtt tgacttcttt acgatataaa cccggcataa
     2341 taggttcaaa ttcgcttaag tagtggaaag taaatgatgg attttggtct gtacttgtca
     2401 aatcaccgga actgaatttc caaaggaaat ttgtggcact tgtagatttg ttaatcatgt
     2461 taaaagtgat gttgaatcga gcttcttcaa tacttatgct tatgataggc atcgttactt
     2521 gaacggtatc ttctactaaa tcgaagtcgg ctgttaatgc tgaatttaca ggtgtaactt
     2581 catttttttt gcatgctaca gcactaacaa ttatcataat gatgagtgct aattttgtga
     2641 tttgtgtttt catttttttg taattaatta gatgaataat tcttttgatt tacaaaacaa
     2701 aattgctata tctgcatcat aagaaaaatt acagttcaag cacattctca ctgaaatgca
     2761 cgttgaaatt gaacgataaa aacaaaaaag aaccacacaa taatcagatt atcaacatga
     2821 taacagagtt accgtatttt ataaaaatag gaatctaacc cttcttttta tttgttaaaa
     2881 aaatgaagaa actcaaacct gatggaaatg ggtaacagca gtgtttattc tgtcttaata
     2941 tccacatcgc cttgtgtacc gattcggaat atcacattaa gcgtatcgtt gctcgggcgt
     3001 gaaatggtgg tacttcggta ataatctgta tagtttacac tatcatacac agccttggat
     3061 actgtaaggg tgttagtggc atgtattgca gcggtgttca cgttcagtaa tttgttgtca
     3121 tacgaagtat ttacagcctc atccaaatct tttaagttaa gtatcactct cgatttggtg
     3181 cttgtttgtg gaatggctac aaattcaagc aaatacatgc ggtgactaaa tgtgtcttga
     3241 tatataatgg aatcgcgata gacaattttt tctacaattt tttctttggt acaacgcacc
     3301 tgaataatca ggaagccaaa tacaagtgtt agaagtaaaa ttttaatttt catatagaaa
     3361 tggtattaaa gatatataat aatctaagtt tttagaatcc tgttcaaatg cttaaatttc
     3421 gttcctcatg tatcagtcaa aactatttga tataatttct gcttttcacc aagctaaaat
     3481 aaataatttt aatcattatc tcaatattta tcggcaaaaa aaagaaaatg cagtaatcag
     3541 acttttcgac tatatatttg aacagtttcc aagcttagac tcacctaagc tggctaaaga
     3601 acaagtacac ggcgttttgt atcctgataa ggaatatgat gagaaaagaa tgcttaacac
     3661 aatgtctgac ttgctaaggt tggcagaaga gtttatttcc tatcaggctt cacgcgagaa
     3721 taaattagaa aacagtctgc atttattgca gttctatctc gaaaatgact tgaacaagca
     3781 gtttgaatct acattcaaag aaacaaagct gagtattgag caaatgcctg aaaatacagc
     3841 atcgctcatt atttcatacc gtatggaatt gcttcatgta gagtatcagc taaaatatga
     3901 caaacgaaat tgtgattatc atgactgtcg cattgcgttg aataacctcg tggtaagcca
     3961 acaaaaaaaa ttagacaatt tatacaatat caatctgtat aatgatcatg actctacctt
     4021 agtcagtagc aagctggcac aaatgcaaga tatgcttcat tatttgttgc taaacgacaa
     4081 taatactgaa ttatatagtg aagcaaaaac attattaaca aaagaagcta agcgttttac
     4141 caaagatgag ttgttgttta tggttataat ttttatcaac cattgtattc gcaaggtgaa
     4201 tgaaaagata gaatcttaca atcaggaatt attgtattgg tacgatttct taattacgta
     4261 caattgtatt ctggaagtaa atgatacgat ttcgtccgct acggtaaaaa attatatcac
     4321 tatctcactt cgattaaatc aactccaaaa agccgacgat tttctggaaa agtatgcttc
     4381 agtattatca gaaacagaga aagaagatgt atataattac aacaaggcta atattctatt
     4441 ccaccaattt aattacgaag aatctttgct tttgctgtca accattaaat ataaagatat
     4501 attttataag ttgagtgcca agcgactgta tatcaaaatc tattacgcac ttacgatcac
     4561 caatgagaaa cgctattcgg atgttatgga cagctcatta aatgccttta aaaaatacat
     4621 ttataccact aaagaaatta ccgaaagcat tcgcacccgc aataagagtt tttacaaata
     4681 ttcggctaag ctggcaaact tacataaagg cgaacaaaaa aaattagaac aattgcataa
     4741 agaaattaca caagacacag aatgtgccga ccgcgattgg ctcttggaaa aaataagtat
     4801 tttactacat taaacattcc cttctttctt cttatacact tcgtctataa accacatctc
     4861 atttttataa ataagattga ctgcatcttg tttggttttt tcatccatct tagaaaataa
     4921 atcatatccc ttttggagca atcctcttcc taaacggatg ccgtccacat cttctttggg
     4981 aaatgtctgt gatttctttt ccatcgtttg tataacgtgg tctaaaatgt cgagtgtttt
     5041 ttttagtaaa tccgtgttga tcatcgcgcc aaacagatgc cgcacatcgc cgataaattt
     5101 ttctacttct tctctaggta gctgataaag ggtgtcttca aaatcttgat cagtcatggg
     5161 cggttgtatg ccattcttat tggctattgc tgccatggag gcttcaaaat ttttagcgcc
     5221 cacaaaagaa tttaacgaat ccataccggc ccgcaatgca gccatgaaat gcctgccgaa
     5281 tttgaaaata gctcgtgcca tatcgccaaa caaaccccat atcttcgagg gtgaacgcac
     5341 gtagctgcct aagtttttaa atgcttcttc tagctgtttc cgctctgctg ccggcgggta
     5401 aataaccgtg agaaaataat ttttgatgct gtttataatt tccggggtaa tcgtgtccgg
     5461 tacatcaaac ctttttttca aatcttcata atcatatcgt ttggagatca tctccctgaa
     5521 tttatttatg atatgttcgt ctttagtttg catagcttaa agttagttaa attctatggt
     5581 tgcagattag gtagtttctt ttgtgcatta tgtttccaat actctattaa gccggagtga
     5641 gtaattccgt tttttaaaag agttggtgta agatgaatgg tctgttggtt tttattttct
     5701 ttccacaaaa tcgacatcgt ttggtagagc tgtttttgaa gtgttttatc atagacaggc
     5761 gtaagcactt caagacggta gtctatattt cgtgtcatta aatcagctga cccgatataa
     5821 tatttggact tgcctccatt actgaaaata aaaatgcggc cgtgttcaat aaactcatct
     5881 gccacactga taattttaat atttttcaat tctttttcgg catagttaac aaaaccgcaa
     5941 attcctcgta cgattagttt tatcttcaca cctgctttag ccgcctgtat cagtttttgt
     6001 tgaatgccct tgtctgcaag gttgtttatc tttaaaaata tttcagccgg ttttttagct
     6061 tttatatgct gaatttcttg ttgtatcagg ctataaagtt gttcacgaag attaaacggt
     6121 gcaataagta aatgtttggg tttgaggtcg gtgctttttt tgtcaaaaaa agcaaatatc
     6181 tcccgggctt ctttggtgat attttgattg gcggtcagca acatgacatc attatacaat
     6241 ttagatgttt tttcatgaag attgccggtg ccaatgcaac cataataaat cttttgaccc
     6301 ttttctctgc gagtaatcaa acataattta gaatgcacct tcatacccgc aacccgtgta
     6361 attactcggg cgccggcttt gcgcagctta ttcgcccaaa cgatatttgc ctcttcatca
     6421 tatctagctc tcaactctat gaatactgtt acatcttttc cgttttgtaa agccgtcatc
     6481 aatgaacgaa taactaatga atcgggtgca acccgaaaca agataatacg gatatgggaa
     6541 acaacaggat cgatggccgc ctcgcgcaat aaatcaagaa cataaataaa tgaatgaaaa
     6601 ggaaaattca gcagaatatc ctttttcttc attttttgca aaatactttt gttgttttca
     6661 aagtcaggat ggttgagatg ctcttgtatg ggatgatgtg ttgaggaaga attaaaaaag
     6721 ggagggataa tgaaattgtc aaaattatgt acacgatcta ctgcaacgaa ttgtacgcct
     6781 ttcgtaacgt ttgttttttt aattagtaaa gacaataatg gcttgggaat agcttgttca
     6841 taaattaaaa actgcgaagt gtttttttta cgtttttcta cgcatttttc cgcggcatcg
     6901 gggaatgtaa cagaaagctc gttatcgatt tccgactcag tgtctttggt atatttcagg
     6961 gtgaacgcac taatggaatc aaacgaataa accttaaaaa tttcaggcaa acaaagtcga
     7021 attacgtcat ctaaaaacaa aacacacagt tctccatata aggatggaag agttaaatat
     7081 ctcgacaaac ggaaagtagg aatttcgagt attgcctgtg cactgattcg tttgtttttt
     7141 ttaagtgcta cagccagata aattgctgtg tcttttaatt gcaatgtctg ggttgatttg
     7201 tccagcatta caggaaatat ttctgcacgc acatgttctg aaaaataatt tcgtacaaat
     7261 gcgatttgtt tcgcatccag ttcttcaggc tgcaaaatgc ggattagttc cttatgtggg
     7321 ttattgggca tgtatatgct ttagaagggc taaaccttca caaattgttt cggatagacg
     7381 aacattatct tttttccatt tttgatttct tgccagttgt ctgtttcaaa ctgcatgcga
     7441 taagcagcgc aggttggaaa tttagcaggt cgttcgtcgg ttaattcaaa aacgatatca
     7501 gcaagaaagt tatcatgccc gaataaaaag acatcccgat aaatattgtt tgtgctttta
     7561 atcagttcaa tgatagaggt tgcatcaccg aaataaattt cctgattgat ttggaagtct
     7621 tccggatggc agcccaaaat accaccaatg ccaatagccg tgtgcaaagc tcgggtagcc
     7681 ggcgaagtgt atattaaatc cgggtgtata ttgttttctt tgagatgatt gccgataagc
     7741 gggattgaca tttcgcctct tccgttcaga ggcctgacca tatcatgcag atttgcattt
     7801 tcccaacaag atttagcatg tctaattaaa gtaagtgttt tcatatcaat ttaggttgta
     7861 aatttaagga ttcaaagcca attaaccatg aacggaaaaa acgatatttt tgaattaatt
     7921 catgcattga gttctaccga gaaatcctat tttaagaaat ttgcttataa aataaaaaca
     7981 aaagacagca aggattattt agctcttttt gatgcacttg tctcacaaaa agaatacgat
     8041 gaacttctgc tgaaaaagaa attaaaacac accaaattat tcaataattt ttccgcttca
     8101 aaaacgtatt taatcaatct tattattgat acgctttcac cctaccagga ggaaaataac
     8161 attcatggta taattttgaa gtcgctttcc caaattcgat ttttaaatga taagaaagca
     8221 gttcagcttg ccgggaaaaa gatacaacaa gtaaaaaaac agattattga gaatgagcta
     8281 tatacttatt cgcacacgct gtttcaattg gaaatagatt taattcccta ttctaagacg
     8341 gaagaaaaat acagaaacca actttttctt gattgccaat atgcagaaag atgcgttcaa
     8401 aattattccg aatatttaca gctcaaagaa gagtggatct atttcagaca aaacacacaa
     8461 acctatacac gcgataattc gttgcaaaac acaattaatg agaaactcaa acacccttta
     8521 ttgcagtcag ctgatacagc actgacaaca agagcaaaat atctatatta ttatatacgc
     8581 ataggatatt acttactgat gaatgatttg gaggctgctt atactgatag ttgcaatcag
     8641 tatgtattgt tagataagaa cctttctttc cgacaacttt atgcaacgga ttatataaac
     8701 aatcttggca accatctctt cagaatgata aatgtaaatc aatatgaaga tgcaaatgat
     8761 gtaattcaaa aattagaatc cgcattgaaa aactatccgg ataaagattt tgtacaatta
     8821 aagcgattga atatactcta tattgaacga gccagaagta tgcatgagaa agactttcct
     8881 cgtatgctgc agaatattga atttatggaa aacgcgattg ggtattttaa aattagaaaa
     8941 gatattgcca tctctcttca atacgatatc atattagcct atttttataa tgataaattt
     9001 gcggatgcac taatgcatgc aaacacgttg ctgaatcatg aagaattaaa tagttttaaa
     9061 gatttggaaa gttatgccca tataattcga ttgttgtgtt tcatagaatt aaataaaaag
     9121 tttgtttctg ataatttgtt tgataatctc aagaagaaac tttatcgtga taagaaatta
     9181 tttaagatgg agaattgtat gcttcggttt ttagaagaat atgctaattc aactgaaagc
     9241 aagcagaaat tagctgtttt gattcaatat aaaaaagaaa tagaacagct cgtacaaaac
     9301 cccttagaac agaacatgct ccattatttc aatattttgt ggtggatgga agccaaaatt
     9361 aaccaaaaaa cgatgctttc ttactttaca aaaacctaag cagtccctaa agaaatacta
     9421 gtataattta ttgattatca aatttctgca tcatgccaaa gaactaccag agcatgaaaa
     9481 aataatgcat ttttgtcctt tttcctgttt ttcacacttt ttatttttta aactagaatg
     9541 taatagaaca agtggggtga ggatttcttt tggtttttag tgtcctagtt tttaaaggaa
     9601 gttagctcca tattgcatca ttacgtttac aaaagttaat acataaatat tagtgcaata
     9661 tgtttgatgg ggatgtttac cctcttgatg agttatgtct atttcagcat gagataaatt
     9721 ttatacataa aagtgaatca ttgaataagc tttccactaa ggatattccc cacttaagaa
     9781 gtgctggggg tgtaacccta ttttatgaca ggacaattta cagaaatccg gtttcctttt
     9841 tttattttag acgcttaatt ctgaaaatat catgcataag aaatttgcgc tcattggcat
     9901 tgccggttat atagcccccc ggcatttaaa tgccatcaaa gatataggag gtgaactcgt
     9961 agcagcgtat gatattgcag attctgtagg aattttagat gcttattttc ctaaatgcga
    10021 attttttaca gacgaatttg cattttatga tttcctgatt gattgtatac cggttgacta
    10081 cttagttgtt tgttctccca attatttaca tgaacagcac tgttatgccg gattaaaatt
    10141 aaatgcggat attatctgcg aaaaaccaac atgcctgacg ctggaaagct ggcaaaattt
    10201 agcggatgcg cagcagtatt ccacaaagaa aatttttacg attttgcaat tgcgccatca
    10261 tcccgaaatt gtagctttaa aaaacagcat tcaaacggat ttatcttatg atgttgaaat
    10321 aaaatatcat actccgcgag gaaaatggta tcataaaagc tggaaaggtg acatgaaaaa
    10381 atcgggaggt attgccacta atatcggcat tcatttattt gatatggtat gctggctttt
    10441 tggagaggta ttagcctttc aagttgaaga aaatacagaa actacctcta agggattgtt
    10501 gcaatttaaa aatgcccgtg taaaatggga tctgaatatt ggcatagaag agaaaccggt
    10561 tcgaagcatt gcgataaatg ctgaacagat tgaatttaca gcgggatttg ccgatctgca
    10621 tacagcatct tatcatcaga ttatggatgg caagggttac ggattggatg atgttaaagc
    10681 ttctattgca ttagtagaaa aaatcagaac gaccgccctt tagcccttac acacaatcgc
    10741 ctttttcttt taccttttga taaaaatatt catattgcgg caagatatta tggatatcaa
    10801 atcgctcggc ctgagccaaa gcatttttgc ggaattgttc taacatcctt tcatctttca
    10861 ataacttgat tgccttggtg gccatacaat ctatatctcc aaccggacat aaatagccgg
    10921 ttactccttc aatattcact tccggtatgc cacctgcatc actcgaaata acaggcactt
    10981 cgcaagccat ggcctctaag gcggccaggc caaaactctc actttccgat ggcatcaaaa
    11041 ataaatcggc aacggccagc aactcctcca ccgcatcttg tttacccaaa aaacgcacat
    11101 caagactatc acataaactg cggacaagca cttccatatt atgtcgctcg gggccatctc
    11161 caataagtaa taattttgca ggcaaagctt ctgcaatttt acagaaaaca taaattacat
    11221 cttctaccct ttttactttg cggaagttgg aagtatgcac aattattttt tcgccattag
    11281 gtgcaatagc tttcttaaaa tgttctttgt tcgttttctt aaaacgcgaa aagtcaatga
    11341 agtttggtat aacttcgatt tctttattga tatcaaaatg cttgtaagta tctattttca
    11401 agctttctga aactgctgta actccacagg agttattgat ggaataagta actacaggct
    11461 catacgtagg gtctttgcct accagtgtaa tatcggtgcc atgtaaggtg gttatcaccg
    11521 gcacatctat gccttttgtt tgaagtatct ttcgagccat aaatgctgcg gaggcatgag
    11581 gaatggcata gtgtacgtga aaaatatcga gattggcata aatggcaaca tccaccagtt
    11641 tgctcgctaa agccgtttcg taaggtgcgt aatcaaacag aggataatca ttgaggctta
    11701 cttcatgata gaaaatattg gcattgaacg aatcaagtaa gcgtgccggt tgtttgtaag
    11761 taataaaatg tactttatgt cccttatctg ccagaccttt gcccagttca gtggctacaa
    11821 cgccactgcc accaaatgta ggataacaca ctattccgat attcatagtc attgtattga
    11881 atgcgtgaag ttaggaaata aagcagatgt atgctttttt cttccgcgag ggatagcata
    11941 attttaagca gaaaaatgag ataaaagcaa ctgcttattc ttaattgttt tcaggagcga
    12001 cggcttcctc agttgttttt acttcttctg cattgttttc ggtaagctca ttatttaaaa
    12061 ctgaatcttc agattgtgtt gtttcggttt cggttggtat atcagagtgc tctgcatctg
    12121 taatagtggt tgcatttgct tctgttgatg tcatgttttc tgtaacagca tgattctctt
    12181 cagtagcagt tgtagcttga acttctttgt cagcgacggc tgtttttact tccttacttt
    12241 catcagcatg atcgttagat ttagcatcag ctttttcagt atgttctttt tgagcttgtt
    12301 cttcttctct ttttttgcgt tgttcttctc gttccgtttg ctgttgtttt tccttttcaa
    12361 tttctttttc caactgctta atcgtatctt ttatgccatc cgcttttttc tctttgtctt
    12421 taatacggtc ggtaatataa ttaaatcggt ttttgatttc atctactcca agtttgccgc
    12481 gagtgtaatg ctgcattttt tcaatttgca tattcagatt atccgtatcg cgtgcaatgg
    12541 agtctttaag tccgtccagt atttgtttga gttgcacaat acggctttgt gtttttgtta
    12601 cagtttcagt agaacgatat ttgcgcaaat catcaaaaat ctgattgata gtagcataaa
    12661 tatttttctt caccgaccaa cgcatggtta catctttgat ttcatgctga atcttcttca
    12721 attcatcaaa aatatatttc cattggtcag agtagattaa ttttgactgt aaatcttcga
    12781 tgcgtttttg tatctgttta gtaaaaacgg aattagcctg atcttcattt tcagtattca
    12841 gcctcttcat cgctttgagt gcatcaaaaa tgccgtttat tttttctttg agcaccgtag
    12901 tttgctccca ggtaatttca tttttctttt ggtaagaagc aattttttcc cattgcgctt
    12961 ttgttttatc ccaaagtgat ttatcatagt ctgatataac atttgcttgt tcttccagca
    13021 attgagtttc gtcagccagc atctggttga tttttgagct catcagtttt ttactccatt
    13081 tgtgttgcga ttgcaatacc aaatccacac gttcaggttt tacttcttcc ggcaatatgg
    13141 tgtcagagtt ggcatcaatt tcccagtgat gggcagtttc gggaaaggca aattcacctc
    13201 cgttcttcca ttcaacaaaa agttggtcct ggatttcttt gctgacttcc tctttaggga
    13261 aagcataaat gtggattttt gtagtaggtt cgtctaaata caaagcaata agtgcttttc
    13321 tgtcggtgcc gatttcgccc cataaaacta atcgtgtgtt catgttgttt aattcaaaat
    13381 tgttcgtatt taccgcattg ctgcgacatc gttttggttg ttaattattt tatttaaaaa
    13441 caaaaatagc ttgctcatca gcaaactatg acacaaaggt attaaatcct gaaataaaat
    13501 actatttttt agttgaaata tggtgatttc cgtttttatt tgccaattgt ccgcatgctg
    13561 catctatatc cttacccctg ctttttcgca cgttggcaat cacgttgttt tgtgttagat
    13621 agccgataaa atgattgcgg tgtatggcat ccgatttttg aaattcagct tgctctattc
    13681 tgttatattc tattacattc acttttactc cttgcaaacg atggcataaa gccaccagtt
    13741 tttttgcatc ttccatgctg tcgttaactc cattaagtag tatatattca aaagtaactt
    13801 cgcctttgca tttcttttga aaataaacta atgcatcaag cagggcttct atattatttt
    13861 gctcattgat gggcataatg ctgtttcttt tttcatcatc tgcagcatgt agcgataatg
    13921 ccagattaaa tcgcacttca tcatctgcca tttttcttat cattttagca atgccggctg
    13981 tggaaaccgt aatacgctta gatgaaaaat tcaatccttc gggcgaagtt attttagcaa
    14041 tggcatccat cacctcgttg aaattcaata aaggctcacc catacccata aaaacgatat
    14101 ttgataaatt gcgcccttgc gtttctagag cctgtttgtt tataagtaca acctgatcat
    14161 aaatttcagc agctgtcagg tttctctccc gtttcaaaaa tccggtggca caaaacgagc
    14221 aggtgaggct gcaccccact tgcgaagata cacaagctgt tgccctatcg tctgtcggta
    14281 tcaacacgcc ttctatcagg tgtttgtcat acaattggaa tgcggttttt acagtgccgt
    14341 cattggagtg ttgagtggta aagatttgta tagggtgaat aacaaaatgt tgttgtagtt
    14401 tttggcgcaa gtctttggat aaatttgtca tggcatgaaa gtctatggca gatttttgcc
    14461 aaagccactc ataaatttgt cttgcacgaa aaggtttttc gcccaattgc tcaagttcag
    14521 caatcaattc atctttagag aatgtgcgga tgtctttttt catgtgtgac ataaaaatac
    14581 cgcctcatag aggcggcata aaaatacaca atatataaaa actgtcaggg gttatttgtc
    14641 cacctgaagt attttgcgat ggtatcttga aattacattc ggatccggat gcaggcgtgt
    14701 agtggcttca tcttttccgt cataaggaat tattgaaagt acataccgaa tggtttccag
    14761 acgtgctttt tgtttattgt tggctcttac aattatccat gggctaaatg aagtatgtgt
    14821 tctgctgaac atctcttctt tgaatttcgt gtaaacatcc cattgttttt gggcttccat
    14881 gtccacgggg cttaatttcc attgtttgag cgggttgcgt ttgcgtgatt caaaacgacg
    14941 caactgctct tcttttgata tggaaaacca gaatttaata agtatcacgc catcttcata
    15001 taacatgtgc tcaaactcgt tcacttgttg cataaagatt tcatactgct gttgagtaca
    15061 aaatttgtta accggctcaa ctacagcacg gttataccag cttctgtcaa aaaacaccaa
    15121 ttctcctctg tttggcaatt gttttacata gcgctggaaa taccattgtc cacgctcttc
    15181 ttctgttggt tttggcaagg ctaccacacg catggtacgc gggttgatat gttctgtaaa
    15241 acgccgtatc gtacctcctt ttcctgctgc atctctgcct tcaaacaaga tggcaacttt
    15301 cgctcctttt tcttgaatcc agcgttgtag ttttaccagt tcaatctgta atttttctaa
    15361 ctcttcctcg tatttaatag agttcaatac tttgtcgggt ttgccattgt tgttatgaaa
    15421 caatttggat aaatccgatt ttgtttttaa gtttttcaac tcacccagcg taacggtttt
    15481 gttttcccat tcattttcgg gtttagttgg gtcttctgct tttgattcag ctggttttac
    15541 tgctgtcttg actggtttta cctgaccatt ggatgttgca gctttcttag tactgcttgc
    15601 tgttgttgtt tttttaactg ccatataata tctgtgtctg ttgttttaaa ggtgctaaat
    15661 tgcaaatata ttacgtcaaa aacaatgata tgtagcaaaa gaaattatga taatcgtcat
    15721 tcgcacagcg gtattttagt atttttgatg catgctacgc aaacttacag ctgatatgat
    15781 ttatcctgtt tccgggcagc cgatagaaaa tggcgttctg attattgacc ataacggtaa
    15841 aattattaca ttaaagaatc gggacgaatt tgctgttgaa gaactggaaa catattccgg
    15901 atatatagtg cctggctttg taaatgccca ttgtcattta gaactttccc atctcaaagg
    15961 tatagctcaa acaggaacag gcttaattga ttttgttaca caagtcatcg ccaaccgcaa
    16021 ccattcgccc gaaattatac aacaagaaat agaaagagca gaaaatgaga tgctacaaaa
    16081 tgggatagtt gctgtagggg atatatgcaa cacgacggat acattttatc aaaagcaaaa
    16141 agaaaatctg tt
//
LOCUS       DAOQII010000025        16003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1737_length_16003_cov_5.213506, whole genome shotgun sequence.
ACCESSION   DAOQII010000025 DAOQII010000000
VERSION     DAOQII010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16003
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1737_length_16003_cov_5.213506"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1057)
                     /gene="lipA"
                     /locus_tag="PLF48_02335"
     CDS             complement(194..1057)
                     /gene="lipA"
                     /locus_tag="PLF48_02335"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNF48189.1"
                     /translation="MTELTTSLPRARKPEWLKVKLPIGENYKKVRHLVDTHKLHTICE
                     SGNCPNMGECWGAGTATFMILGNVCTRSCSFCAVATGRPKFVDFDEPKRVAEAVKLMG
                     VRHAVITSVNRDELPDKGAQIWHDTVKYIKEYAPETTIETLIPDVKGDWKSLALMISA
                     GQEVVSHNMETVKRFYKRVRPQAKYERSLEQLLRIHEFGKRSKSGIMLGIGETQEDVY
                     ELMEDLARQKLNVLTLGQYLQPTKMHLPVAEYIHPDLFKHYHDIGMTKGFDVVESGPM
                     VRSSYHAEKHL"
     gene            complement(1059..1907)
                     /locus_tag="PLF48_02340"
     CDS             complement(1059..1907)
                     /locus_tag="PLF48_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48190.1"
                     /translation="MKKYIRIFPVLIFLLSLCKSVSAQETTLNPDSVLYKKAWQAGLR
                     LKSDGFSLNAEIARSKRFKREFIIQVGFSFYLSPKQVKQDSPLGGGGLFGSDGFKRFV
                     YGKQNNQLNLYAGVGQRFLLAEKARKHGVQIFYKYAGGITLAILKPYMLKIAQLDTSA
                     PNTIVGFEDVQFDKNNLDNGFLYPFPYVTNDLKFIAGASGFGKGWKLKLVPGLHIETG
                     IDFDFGKNDRFIKALEVGVMCDFYYKKLPVMVDIDIDGQSIKNSNKFMYPSVFVGLSL
                     GKRKMK"
     gene            1946..2359
                     /locus_tag="PLF48_02345"
     CDS             1946..2359
                     /locus_tag="PLF48_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF48191.1"
                     /translation="MATAKVTYLGELRTEMQHLQSGSLVITDAPVDNHGKGEAFSPTD
                     LTATSLASCMFTIMGIKLQSMQKDIKGATSEVTKVMYDDPRRIGEILIDIKIPEQDFT
                     DKEKQMLINITKTCPVAKSLHPDIKLVVNVSFTKP"
     gene            complement(2380..3036)
                     /locus_tag="PLF48_02350"
     CDS             complement(2380..3036)
                     /locus_tag="PLF48_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF48192.1"
                     /translation="MDNANKRTNINVFCFNPFQENTYIISSPEKNCWIIDPGCFTHQE
                     EQILTDFISKNDLKPVRLLNTHCHLDHIYGNKFVAEKYGIELAIHEKEMPILEKAALS
                     ARLFGAKIPEHLEPRYFLQEGELLKLDDAVFEVLFCPGHSPGGICLYNKAEKYCIVGD
                     VLFEGSIGRTDLPLGDHHTLLQSIHSKLMSLDDEVIIYSGHGNPTTIGRERKFNPFLN
                     "
     gene            3057..6566
                     /locus_tag="PLF48_02355"
     CDS             3057..6566
                     /locus_tag="PLF48_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48193.1"
                     /translation="MYRNNPVKNSRGMYRNFLDQLRFSISLCCFPAVIIFSFISVFFQ
                     AVTAQTTQVQFGQNRVQYHPFDWQYYNSDNFTIYFYPGGQEIGKFIWTTAEMKVKELE
                     DDLEFRFNDKIDILVYNDITDMAQTNIGLGQEEFNLGGTAVLKNNKLFLYYDGNHQHL
                     ERDLMKGMAEVMLRQMMTGSNFKQKIQNSVFLNLPEWYRKGLSAYIAENWNTVNDNRL
                     KNYLANNKKTSFSKLAAQDPEFAGQSFWNMVREKYGSDAIQNILYLTRVKHNMNSGFA
                     YAINTTYNDLIADWKTYYSNRFKEDEENRTALQNAITLKLKKRNNTDIGDMVISADGN
                     YLAYAVFDGGFYKIFVQNTQTKKVKKIAKGGFHSDQYPFDKSYPILTWSKTGNKLVVI
                     HEKKDKIRLLQFDVALKEKVKKNIIKFQRIYGADFTNNPNLLVLSGQNKGQTDLYLYN
                     ISNSNSTQLNNDLYDDLNPVYVHLNESDGILFSSNREDDKLYFEVNDTVLPDKSLDLF
                     YYDLNKRGDELARVTETPFANEKGTGSYIENKFSFLSDENGISNLYTGTIDSFFIRVD
                     TLDVWENESTDSAIAVKKAVDIYKLKGITSPLTNLPNSILASGNSNAKTFYFFGVKRK
                     KTSVSFTSKADEEQLENAKKFGLTPTTFRRKYLTTATDVSSPAKTSILSNKQQEIKNT
                     KAQNSGGITVDSLLSETFTYTFESDFNNTLKSNEQKNSDTGITTILSDTTVTFGNTDE
                     EAPPPFIPPSMLNVERTTGKKIRILSYRARFSSDYVVTQIDNSILTTNYQSYNANLGG
                     YSFPNLSGMIAFGTSDLMEDHKIIGGFRFPFDFKGLETFVKYINLKHRLDHSILYYRK
                     SDRTYFNINNPYTPDATYTGKTRMNFLEYTMSYPFDITKSLKAAFSYRNERLDVSYSD
                     PITLAVNDIKENWISSRVEFVHDNSKEIQTNIYNGYRYKVFGEYFYNANEKKSHLFNL
                     GFDVRHYQKIFRNLIWANRLAGATSFGNKKILYYLGGVDSWLNPKYDYNIPVSSTQNY
                     GLQAFATNLRGLPQNIRNGNNYLLLNSELRFPVISFFAKKPLKSSFLQDFQLVGFFDA
                     GIAYTLLNPFDRNNSISQEVVSEDPLVISVNYYRNPFVFGCGGGMRTNILGYFLRVDA
                     GWGYDGSKLAQKPIWHISLSKDF"
     gene            6744..7685
                     /locus_tag="PLF48_02360"
     CDS             6744..7685
                     /locus_tag="PLF48_02360"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNF48194.1"
                     /translation="MALSGTHFTFPNQTSFYKGKVRDVYTIANETLVMVASDRISAFD
                     VVLPRPIPYKGQVLNQIAAKFLDATKDILPNWVSAVPDPNVTVGQKCEPYKVEMVIRG
                     YLTGSAWRAYKNGSRVLCGVTLPDGLKENQQFEKPIITPTTKAEVGHDEDISKEEIIA
                     QGIVSKEEYEQLEVYTQQLFQRGTEIAKERGLILVDTKYEFGKIGNTIYLMDEIHTPD
                     SSRFFYIDGYEQAYEAGATPKQLSKEFVREWLIANDFMGKEGQTVPEMTDEIVQSISE
                     RYIELYEIITGEKFIKSDDADILNRIETNINSYLNAH"
     gene            complement(7850..9082)
                     /gene="serA"
                     /locus_tag="PLF48_02365"
     CDS             complement(7850..9082)
                     /gene="serA"
                     /locus_tag="PLF48_02365"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNF48195.1"
                     /translation="MTKQTSFPKEKIKILLLEGLHQSASNEFHKKGYTSIDSHKEAMT
                     EQELLDCIEHYHIIGIRSKTQLTAAVLEKAKKLLTIGCFCIGTNQVDLAKATELGIPV
                     FNSPYSNTRSVAELIIGNSIMLLRRIPEKNKKMHEGNWQKTAKGSYELRGKTLGIIGY
                     GHIGSQVSVMAESLGMKVIYFDIEPKLPLGNADAVDTLDELLQKADVVTLHVPATAET
                     KNMIGENEFALMKEGVIFQNLSRGTVVDIPAMKKALESGKIAGAAIDVFPTEPKQNGA
                     GFESDLLGFDNVLLTPHVGGSTQEAQENIGTEVAVKLTGFLDNGSTLGSKTVPEISLS
                     VQQNTRRILHIHKNVPGVLSEINAILSKHEVNILGQYLKTNETIGYVVLDVDANADLE
                     MMEDLKQVKETIKSRILY"
     gene            9457..10686
                     /locus_tag="PLF48_02370"
     CDS             9457..10686
                     /locus_tag="PLF48_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNF48196.1"
                     /translation="MPETQTNNNGMQLLLQLKNNQMNALLEATKAINDNVSSEGLYQL
                     YKYAIEENLGVSDVALFMFDQTWKRVVWQRTQQTDTFIPVNAWFEQYKNTQELTKEEQ
                     ASFSGFKYIIPVYHKQQPLALALLGEIQEDNIHHKDELLEFAQVFTNVITVAVENKRL
                     FKRELEKKQFDKELELASKVQGMLIPNKLPKNTMYEFAGLYLPFKGIGGDYYDVIHIN
                     KDEFVFCIADISGKGVAAALVMANLQAYLNATLGLGFSKEKLIEKLNQKIYSITNGEN
                     FITLFIAKYNIISRELEYLNAGHIPPVLVHGNRVELLECGTTLLGIFEELPKIEFGKI
                     HIKPDSTILCFTDGLSELENSDDEVMGVERLKQFALTHHKLGAELFNKILFDSISKFK
                     GNALFNDDISVLTAKIF"
     gene            complement(10673..12136)
                     /locus_tag="PLF48_02375"
     CDS             complement(10673..12136)
                     /locus_tag="PLF48_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNF48197.1"
                     /translation="MQTASDSNAIDFIQRHATSAFWTIASLSLVLLITAFIYKIPEAA
                     LLPFALLFGLLTLLNFRIVFYIMLCVMPFSIEYTFGSLGLDIPSEPIVMLLCVCTVIY
                     LVYRYDLLRNAAFQHVITTLIVLQFLWSGVAAIYSANLLLSVKYLLAKFWYITVFFVL
                     PLLFIKTEKDIHKFFWCVFIPTFCSVCYVMVRHALYGFTFDTITQSVHPIYRNHVNYA
                     VFITMIFPFIPMAMNWYKKDSFIYKFLRLSIPLFITAIYFSFTRGAWLAFLAMGVYYL
                     IMRFRLTKLVLVISAFCILIFSIYILHDNTYLKYAPNYENTIYHEELSEHLSSTFEME
                     DMSTVERFYRWIAAVHLFKKHPLTGVGPNNFVSQYKKYTVTAYETYISDNEEKSTVHN
                     YFLLLLTEQGVPAFILFVLLIAALLITAENAYHQINHAQKKYALAFTLCIVVFLINNS
                     LSDLVEANKVGSLFYICAAVLVNLAVLASREKQAKIS"
     gene            complement(12105..12896)
                     /locus_tag="PLF48_02380"
     CDS             complement(12105..12896)
                     /locus_tag="PLF48_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48198.1"
                     /translation="MKLVYKWKKHILLFCLFAVIVSIILTMPSIMPPYYKSKMIFYLS
                     NPVSTDRAALFNEKEAGGVYPFGGKEEINRMLTILTSEPAITHIIDKYNLSKHYDIEK
                     DESGLYRYYTALEFNSNFDAIRNDNGALEVTFMDRDKKLAAAVVKDIVQYADSVYRNM
                     LLVNKTNVLTLLDEQIAQKKANGSDAEDLNKFITIRDQYTVASSNTFKTMYVVENPTP
                     AIKKCKPIRWLIVLSTLIGSFILACFTALIIELYKNADRFGFQRD"
     gene            complement(12996..14276)
                     /gene="tyrS"
                     /locus_tag="PLF48_02385"
     CDS             complement(12996..14276)
                     /gene="tyrS"
                     /locus_tag="PLF48_02385"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNF48199.1"
                     /translation="MKSFVEELKWRGMLHDIMPGTEDLLNKQMVKGYIGFDPTADSLG
                     IGNMVQVMTLLHFQRCGHKPIALVGGATGMVGDPSGRSSERNMLNEEELNYNLSCQRK
                     QLEKFLDFDCGENSAEIVNNYDWFKEFSFLQFIRDVGKYITVNYMMAKDSVQNRLESG
                     MSFTEFSYQLVQGYDFYHLWKNNNVMLQMGGSDQWGNIVTGTELIRKKEAGEAYALTT
                     QLIKKADGTKFGKSESGNIWLDKNKTSPYKFYQFWLAADDKDVKTLFRIFSLKNREEI
                     EALEQQHEGNEHLRILQKALAEEITERVHSHADMVHAQQLSAILFDKDVKSKIMELEE
                     TDFLEIFEGVPTFVISQNDIEKGIPILDLLAEKTTIFPSKGEARKLMQGNGLSLNMEK
                     FADVQAIISSADLIKNKYLLIRKGKKDDKLVVVE"
     gene            14443..15663
                     /locus_tag="PLF48_02390"
     CDS             14443..15663
                     /locus_tag="PLF48_02390"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNF48200.1"
                     /translation="MIKQKGHIYLLLVVIILGIYTASAKPPKYSNDFMYIGVGANNFG
                     LGNAVVANTGDITSGYWNPAGLVRIQTNLQFAYMHNEYFASIAKYDYAGIGVPLDSNK
                     HAFAINFMRFGVDDIPNTLDLIDADGTVNYDNVKNFSVGNYAFLLSYARNIIKGLSVG
                     ATAKIVHNKAGGFAKSWGFGVDIGAIYQIKDWKIGLTVKDITTTFNAWSFSFTDKEKQ
                     ILDATDNVIPVSSTELTLPRIQFGASYNKTFKDKIELMAELDWELTTDGKRNTLISSK
                     AISLDPRLGVELGLWKIGYLRGGISNFQKMTNLSTGKQKFTVQPSIGVGLRYKNIVYL
                     DYAFTDVGKTSENQYSHVISLKVGINKRKHKENAELINAAPQENVSGQEQPASQPTEI
                     QSPEKENNNNAQPK"
     gene            15663..>16003
                     /locus_tag="PLF48_02395"
     CDS             15663..>16003
                     /locus_tag="PLF48_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48201.1"
                     /translation="MKKLLAIVFLITLCRSLGFAQPYGNEWIDYNKTYYKFKIAQDGL
                     CRINYATLIATGIPAAQLKGTDFKLICNGEEIPIYVTTANQFTASDFIEFYGEKNDGS
                     VDTRLYKNPLE"
BASE COUNT         5169 a         2984 c         2870 g         4980 t
ORIGIN      
        1 agcgtttttt tcatatcaat ttatttagta caaaattaca aatagttttt catactaatg
       61 attatccatt cacttttatt tttttaacca tttaatgtag tatttataaa atccgtttat
      121 tctattacta caaaattagt ttaatccttt gttggattaa tctaaaaatt ctcttggaaa
      181 tatatagaat gttttacaaa tgcttttcgg catgataaga ggagcgcacc ataggtccgc
      241 tttccaccac atcaaaacct tttgtcatac ctatatcgtg atagtgtttg aataaatcgg
      301 ggtgtatata ttcggcaaca ggcaaatgca tttttgttgg ttgtaaatat tgtccaaggg
      361 taagcacatt taatttttgt ctggctaaat cttccatcag ttcatacacg tcttcttgtg
      421 tctcaccaat acctaacata atgccgcttt tgctgcgttt gccaaactca tgtattcgca
      481 aaagttgctc aaggctgcgt tcatatttag cttgtggtct cacccttttg taaaatcgtt
      541 taacagtttc catattatgc gataccactt cttgtccggc actaatcatc aacgctaagc
      601 ttttccagtc gccttttaca tctggaataa gcgtctcaat ggtcgtttcg ggtgcatatt
      661 ctttgatgta ttttacggta tcatgccaaa tttgtgcgcc tttatccggt agttcatctc
      721 tgtttacgga tgtaatgact gcatgtctta ctcccattag ttttacagct tcagctactc
      781 gtttaggttc atcaaaatct acaaattttg gtcgccctgt ggctactgca caaaatgaac
      841 aagagcgtgt acatacattg cccaaaatca taaaggttgc tgtgcctgcc ccccagcatt
      901 cgcccatatt cggacaattg ccactttcgc aaatcgtatg gagtttgtga gtatccacta
      961 aatggcgaac ttttttataa ttttcaccta tgggtaattt tactttcagc cattccggct
     1021 tccgggctct gggtagtgaa gttgttaact cagtcatttt acttcatttt tcgttttccc
     1081 aatgacaagc ccacaaaaac actcggatac ataaatttat tgctgttttt tatgctttgc
     1141 ccatctatgt caatatcgac catgacgggc aattttttat aataaaaatc acacatcacc
     1201 ccaacttcca gcgctttgat aaacctgtcg tttttgccaa aatcaaaatc aattccggtt
     1261 tcaatatgca aacccggaac aagcttcaat ttccatcctt ttccaaatcc ggatgctccg
     1321 gcaataaatt ttaaatcgtt agttacatac gggaaagggt ataagaaacc attatcaaga
     1381 ttatttttat cgaactgcac atcttcaaat ccgactattg tattaggagc actggtatcg
     1441 agctgtgcta ttttgagcat gtaaggtttt agaatggcaa gcgtgatacc tcctgcgtat
     1501 ttataaaata tctgaacacc gtgtttacgg gctttttctg ccagtaagaa acgctgacca
     1561 acacctgcat acaaattcag ttggttattt tgtttgccat agacaaatct cttgaaacca
     1621 tctgaaccaa acaaacctcc tccacccagc ggagaatctt gttttacttg ttttggtgac
     1681 aaataaaatg aaaaacctac ctgaatgata aactctcttt taaatctttt actacgagca
     1741 atttcagcat tcaggctaaa accatcactc ttcagacgca atccggcctg ccaggctttt
     1801 ttatacaaaa cagaatcagg atttaatgtg gtttcttgtg ctgatacaga tttgcataag
     1861 cttagcaaaa aaatgagtac cggaaatatg cggatatatt ttttcaaaat cggatatttg
     1921 gctaaattag tttaaaattc ataaaatggc aacagcaaaa gtaacatatt taggagaatt
     1981 acgtaccgaa atgcaacatc tccaaagtgg cagtctggta attacagatg ccccggttga
     2041 taatcatgga aaaggtgaag cattttcacc aacagacttg actgccactt ctttggcatc
     2101 gtgtatgttt accataatgg gtataaaact gcaaagcatg cagaaagata ttaagggtgc
     2161 tacttcggaa gtgactaagg tgatgtatga tgaccccaga cggattggcg aaattttgat
     2221 tgacataaaa attccggaac aagattttac cgataaagaa aaacaaatgc tgataaatat
     2281 tacgaagact tgccccgttg ctaaaagctt gcatcccgat atcaaactgg tggtgaatgt
     2341 atcatttaca aaaccttagt ttcaagaaaa tcaggtcagt taatttaaaa acggattaaa
     2401 cttgcgttca cgaccgatag tagtcgggtt gccatgtccc gaataaatga ttacttcatc
     2461 atcgagtgac atgagcttac tgtgaattga ttgcaaaagt gtatgatgat ctcctaaagg
     2521 caagtccgtt cttcctatgc tgccctcaaa aagcacatcg cctactatac aatatttttc
     2581 agctttgtta taaaggcaaa tacctccggg agaatgcccg ggacaaaaga gtacctcgaa
     2641 tacagcatca tccagtttga gaagttctcc ttcttgtaaa aaatagcgag gttccaaatg
     2701 ttcggggatt tttgctccga aaagccgggc tgacaatgct gccttttcta aaatgggcat
     2761 ttccttttca tgaatagcca actcaatgcc gtatttttct gctacaaact tattaccgta
     2821 tatatggtct aaatggcaat gcgtattcag taaacgaaca ggctttaggt catttttgct
     2881 gataaaatca gtcaaaatct gctcttcctg atgcgtaaaa catcccgggt cgattatcca
     2941 gcagtttttt tcgggacttg aaatgatata ggtattttcc tgaaagggat taaaacaaaa
     3001 tacgttaata ttggttcgct tatttgcgtt gtccatatta tttatttact ttgtaaatgt
     3061 acagaaataa tcctgtaaaa aacagccgag gtatgtaccg aaatttttta gaccagttgc
     3121 gatttagtat ttcattatgc tgttttccgg cagtaatcat tttttctttt atatctgttt
     3181 tttttcaagc agtcactgca caaaccactc aagttcagtt tggtcaaaat cgggtgcagt
     3241 atcatccgtt tgactggcaa tattacaact ctgacaattt tactatttat ttttatccgg
     3301 gtggtcagga aataggcaag tttatctgga cgactgctga aatgaaagta aaagaactgg
     3361 aagatgattt ggaatttcgt ttcaacgata agatagatat tctggtttat aatgacatca
     3421 cagacatggc acaaaccaat atcggcttag gtcaggagga gtttaatctg ggtggaactg
     3481 ccgttttaaa aaacaataaa ttatttcttt attatgatgg taaccatcag caccttgaac
     3541 gtgatttaat gaagggtatg gctgaggtga tgctgcgcca aatgatgacc ggatcaaatt
     3601 tcaaacaaaa aatacaaaat tctgtatttc taaatttgcc tgaatggtat agaaaaggac
     3661 tttctgctta tatcgctgaa aactggaata ccgtaaatga taatcgctta aaaaattatc
     3721 tcgccaataa caagaaaact tcattttcaa aattggctgc tcaagacccc gaatttgcag
     3781 gtcaatcgtt ttggaatatg gtaagagaaa aatatggtag tgatgccatt cagaacattc
     3841 tgtacctgac acgtgtaaaa cacaatatga acagcggttt tgcatatgcc attaacacga
     3901 catataatga tttgattgct gactggaaga cttattattc caatcgtttt aaagaagatg
     3961 aagaaaacag aacggctttg caaaatgcca taactttaaa actgaaaaaa aggaataata
     4021 cggatattgg cgatatggtt atttctgctg acggcaacta tttggcttac gcagtttttg
     4081 acggaggttt ttacaaaata tttgttcaaa atacgcagac gaagaaagta aaaaagattg
     4141 ccaaaggcgg atttcattcc gaccaatatc cttttgataa atcttatccg attttaactt
     4201 ggtcaaaaac cggaaataag ttagtagtca ttcatgaaaa gaaagacaaa atccgattgt
     4261 tgcaatttga tgttgctttg aaagaaaaag tcaaaaagaa tatcatcaaa tttcaacgaa
     4321 tttacggggc tgatttcact aataatccaa atttacttgt tttgtccgga caaaataaag
     4381 gacagactga tttgtactta tataatatca gcaattcaaa ctctacacaa ctcaacaatg
     4441 acttgtatga tgatttgaat cctgtttatg ttcatttaaa tgaaagcgac ggcatcctgt
     4501 tttcgtctaa cagagaggac gacaaacttt attttgaggt aaatgacact gttttgcctg
     4561 ataaatcgct tgatttattt tattacgatt taaataaacg cggagatgag ctggcacgag
     4621 taacagaaac tccttttgcc aacgaaaaag gaacaggtag ctatatcgag aataagtttt
     4681 cttttttaag tgatgaaaac ggcatcagca acttatatac aggaactatt gacagctttt
     4741 tcatcagagt tgacacttta gatgtttggg aaaatgaaag tacagattct gccatagcgg
     4801 taaaaaaagc ggttgacata tacaagctaa aaggaattac ttcaccactt actaacctgc
     4861 caaattccat attggcttct ggcaacagta atgccaagac tttttatttc tttggtgtaa
     4921 aacgcaaaaa aacaagtgtc agcttcactt caaaagcgga tgaagagcaa ctggaaaatg
     4981 caaaaaaatt cgggctcact ccaacaacat tcagacgaaa atatttgact acggctaccg
     5041 atgtgagctc acctgccaaa acaagtatct taagcaataa acaacaggag ataaaaaaca
     5101 caaaagcaca aaacagcggt ggtataacgg ttgacagttt actttctgaa acatttacct
     5161 atacctttga atcagacttt aacaacacac tgaaatcaaa tgaacaaaaa aacagcgaca
     5221 cgggaattac tacaatctta agcgatacaa ctgtcacatt tggcaatacg gatgaagaag
     5281 ctccgcctcc atttataccg ccgagtatgc tcaatgtaga gcgcactacc ggaaagaaaa
     5341 tccgcatttt gtcctatcgt gccagattca gctctgatta tgtggtaacc caaattgaca
     5401 acagtatact cactaccaat tatcagagct ataatgctaa tttgggtggc tattctttcc
     5461 caaatttaag cggtatgata gcattcggca cttcagattt gatggaagac cataaaatta
     5521 tcggtggttt caggtttcct ttcgatttca agggattaga aacttttgtc aagtatatca
     5581 acttaaaaca cagattagat cacagcattt tgtattacag aaagtctgac agaacctatt
     5641 ttaatatcaa taatccctac acaccggatg ctacatacac cggaaaaacg cggatgaatt
     5701 ttctagagta caccatgagt tatccgtttg acattacaaa gagtctgaaa gcagcttttt
     5761 cttaccgaaa cgaacgatta gatgtttcct attccgaccc gataacttta gcggtgaatg
     5821 acatcaaaga aaactggatt tcttcacgag tagaatttgt acatgacaat tccaaagaaa
     5881 tacaaaccaa tatttataac ggttatcgtt acaaagtttt tggagagtat ttttataatg
     5941 ccaatgaaaa aaaatcgcac ttatttaatc ttggatttga tgttcgccat tatcaaaaaa
     6001 tattcagaaa tctgatttgg gctaatcgac ttgcaggagc tacttcgttc ggcaataaaa
     6061 aaatattgta ttatttagga ggtgtagatt cgtggctgaa cccgaaatat gactataata
     6121 ttccggtgag cagcacacaa aactatggtt tgcaagcctt tgctactaac cttagaggac
     6181 taccgcagaa catcagaaat ggaaacaatt atttgttgct gaattctgaa ttgcgttttc
     6241 ctgttatttc attttttgcc aaaaagccac tgaaatcttc tttcttgcaa gacttccagt
     6301 tagtaggatt ttttgatgcc ggaattgcct atacgttact aaatccattt gacagaaata
     6361 attcaatcag ccaagaagtt gtttctgaag atccattagt aatatctgta aactattatc
     6421 gcaatccatt tgtatttgga tgcggtggcg gcatgcgcac aaatatttta ggttatttct
     6481 tgcgtgttga tgccggttgg ggttatgacg ggtcgaaatt agcacagaaa ccaatttggc
     6541 atatttcgct tagtaaagac ttttagttgt atttgaaatt ttattagtaa ttggtttaat
     6601 agtttattag tttaaatgtt ttttagtttt ttaaaggatt tagggttttc ttgtttggtt
     6661 tgatgattgt gtacaaccat cttaattgtg gatagcttga attgattttt tcttattgtc
     6721 aaattacctt tgcgaaaaaa actatggcac tttccggcac gcattttaca tttcccaatc
     6781 aaacatcttt ttataaaggt aaagttcgcg atgtatatac tattgccaat gaaactttag
     6841 taatggtggc ctccgaccgc atttctgcat ttgatgttgt tttacctcgt ccaatcccat
     6901 ataaaggaca agtcctcaat cagattgcag caaaatttct ggatgctacc aaagacatat
     6961 tacctaattg ggtgagtgca gttcccgatc caaatgttac ggtgggacaa aaatgcgaac
     7021 cctataaagt ggaaatggtg attcgtggct acctgacagg ttcggcatgg cgtgcgtata
     7081 aaaatggcag tcgtgtgctt tgcggagtta ctttgccgga tggactgaaa gaaaaccaac
     7141 aatttgaaaa acctatcatc acaccaacta ctaaagcaga agtgggacat gacgaggaca
     7201 tttcaaaaga agaaataata gcacagggca ttgtatcaaa agaggagtat gaacaattag
     7261 aagtgtacac tcaacaattg tttcagagag gcacagaaat tgcaaaagaa agaggtttga
     7321 ttttggtaga tacgaaatat gaattcggaa aaattggaaa taccatctac ttgatggatg
     7381 aaattcatac accggactct tcccgctttt tttatattga cggatacgaa caagcttatg
     7441 aagccggagc aactcccaaa caattatcta aagagtttgt acgggaatgg ctgattgcca
     7501 atgatttcat gggaaaagaa ggtcaaacag ttcctgaaat gactgacgaa atcgtacaat
     7561 ctatttctga aagatatatc gaactctatg aaatcattac cggagaaaag tttatcaaaa
     7621 gtgatgatgc tgatatttta aacagaatag aaacgaacat aaattcgtat ttgaatgcgc
     7681 attaaaataa actactaacc tgagttctgg ataagcgttt ccattaaaaa atagttgatt
     7741 ttttatgtag aaccgcctcc ttttaattcc tccaccttgt taaagtggag gtaaaaatga
     7801 gaaattaact attttatcag ctagtttttc ttaacccgaa ctcaggttac taatacagta
     7861 ttctggattt tattgtttcc tttacttgtt tcaggtcttc catcatttcc aaatctgcat
     7921 tggcatccac atctaatacg acatagccaa tagtttcatt tgttttcaag tattgaccta
     7981 aaatattgac ttcatgttta gacaatattg cattgatttc ggataaaaca cccggtacat
     8041 ttttgtgaat gtgcaatatt cttctggtat tttgttgtac acttaagctg atttcaggaa
     8101 ctgttttaga tcctaaggtg cttccattat ccagaaaacc agtaagcttt actgctactt
     8161 ccgtaccaat attttcctgc gcttcttgcg tacttccgcc aacatgcggg gtcagtagta
     8221 cattgtcaaa acccaataaa tctgattcaa aacctgcgcc gttttgtttt ggctctgttg
     8281 gaaatacatc tatagcggct ccggctatct taccactttc taatgctttc ttcattgccg
     8341 ggatgtcaac cacggtaccc ctggatagat tttgaaagat aactccctct ttcatcaatg
     8401 caaattcatt ttcgcctatc atgttttttg tttcagccgt tgccggaaca tgtaatgtaa
     8461 ctacatctgc tttttgcaac aattcatcaa gcgtatctac agcatcagca ttacccaaag
     8521 gcaacttcgg ttctatatca aaataaatca ccttcatacc caaactttcg gccatcacag
     8581 atacctgtga accgatatgt ccgtaaccga taatgcccaa tgttttgccg cgaagttcat
     8641 agctgccctt agctgttttt tgccagttgc cttcgtgcat ttttttattt ttttccggaa
     8701 tgcggcgcag caacataatc gaatttccaa tgataagttc tgcaacagag cgtgtattgg
     8761 aatagggcga attaaatacc ggaatgccca actctgtagc cttagctaaa tcaacctgat
     8821 tggtgccgat acaaaaacat ccgatggtta aaagtttctt tgctttttcc agtacggcag
     8881 cagtaagctg tgttttggaa cgaatgccga taatatgata atgctcaatg caatccagta
     8941 attcctgctc ggtcattgct tccttgtgac tgtcaatgga ggtatagcct tttttatgaa
     9001 attcattgga agcagactgg tgtaatcctt cgagcaacag aatttttatt ttctcttttg
     9061 gaaatgaagt ttgttttgtc ataatcaaat ttatgcattt ttagcagggc tttattccgt
     9121 ttgagaataa gaaagtttta tgaaaagtat atcaatactt aatacaatgt cattaattta
     9181 ggtatgattt aatgttgaat atccaaaaat agttgatctt attattttag aacctcctcc
     9241 tattattcct ccgcattgat gaaggcggag gataaaatgt aaaattaact atttatacag
     9301 ccagctaact ttaacgacat tgatacttaa tacaccaaaa ttcaacccgg tgtaagtctg
     9361 tctcaccgaa taaggtgatt ttttgtttgt gcgaaatttt tagtggcttc atccctaatt
     9421 tatttcctct cttttccgta aattactcat caaattatgc cggaaacgca aactaataat
     9481 aatggtatgc aattgctgtt gcaattgaaa aacaatcaga tgaatgcctt gctggaagcc
     9541 acaaaggcca tcaatgataa tgtttcttca gaaggtttgt atcagctata caaatatgcc
     9601 attgaagaaa atctgggtgt ttcggatgtc gccttattta tgtttgatca gacctggaag
     9661 cgagtagttt ggcagcgtac ccaacagaca gacacattta taccggtaaa tgcctggttt
     9721 gagcaatata aaaacacaca ggaactgact aaagaagaac aagcttcgtt tagtggcttt
     9781 aaatatatta ttccggttta tcacaaacaa cagcctcttg ctcttgcctt gcttggcgaa
     9841 atacaggaag acaatattca ccataaagac gaattattag aatttgctca ggtctttacc
     9901 aatgtaatta ccgttgctgt cgaaaacaag cgcctgttta aacgagaact ggaaaaaaaa
     9961 cagtttgata aggagctgga actggcatca aaagttcaag gcatgctcat tccaaataaa
    10021 ctacccaaaa atacgatgta tgaatttgcc ggtttgtact tgccgtttaa aggaattggc
    10081 ggtgattatt atgatgtcat tcatatcaat aaagatgagt ttgttttttg tatagctgat
    10141 atttcaggga aaggtgtggc tgcggcattg gtaatggcca atttgcaagc ctatctgaat
    10201 gccactttag gtttgggttt ttcaaaagaa aaactgatag aaaaactcaa tcaaaaaatt
    10261 tattcaatca ctaacggaga aaatttcatt accctgttta ttgccaaata caatattatc
    10321 agcagagaat tggaatacct gaacgcaggt catattccac cagttttagt acatggaaat
    10381 cgggtagaat tactggaatg cggtactaca cttttgggta tttttgaaga attgccaaaa
    10441 atagaattcg gcaaaatcca catcaaaccg gattcgacaa tcttatgttt taccgatggt
    10501 ttgtctgagt tggaaaacag tgatgacgaa gtgatgggag ttgaacggct gaaacaattt
    10561 gcacttaccc atcacaaact cggagcagaa ttattcaaca aaatattgtt tgacagcatc
    10621 agtaaattca agggcaatgc tctttttaac gatgacatca gcgtattgac tgctaagata
    10681 ttttagcttg tttttccctg cttgccaata cagctaaatt aaccagtact gctgcacaga
    10741 tataaaaaag cgaacctact ttattagctt caaccaaatc actcagacta ttattaatca
    10801 aaaaaacgac aatgcaaagt gtaaatgcca gcgcgtattt tttttgagca tgattaattt
    10861 gatgataggc gttttcagcc gttatcagca acgctgcaat cagcaaaaca aacaagataa
    10921 aagccggaac accttgttcg gtcaataata gtaagaaata attatgcact gtagattttt
    10981 cttcattatc actgatatat gtttcatagg ccgtaaccgt gtatttttta tactgcgaaa
    11041 caaaattatt cggtccgact cctgttaaag gatgtttttt aaataaatgc acggccgcaa
    11101 tccatctgta aaaacgttca actgtactca tatcttccat ctcaaaggta gatgacaaat
    11161 gctcactcag ctcttcatga taaatggtgt tctcataatt aggagcatat tttaagtaag
    11221 tgttgtcatg caagatataa atactgaaaa taagaataca aaatgcactg attaccaaaa
    11281 ctaattttgt caaacggaaa cgcataatta aataatacac acccatggct aaaaatgcga
    11341 gccatgcacc acgtgtaaat gagaaataaa tggctgtaat aaaaagtgga atgcttaatc
    11401 tcaaaaattt gtagataaaa gaatcctttt tataccaatt catagccatg ggaataaatg
    11461 gaaaaatcat ggtaataaac acggcataat tcacatgatt ccgataaata ggatgaacag
    11521 attgcgtgat ggtatcaaat gtaaatccat ataatgcatg tctcaccatc acataacaaa
    11581 cactacagaa tgtcggaata aatacgcacc aaaaaaactt gtgaatatct ttttcagttt
    11641 ttataaataa taatggcaac acgaaaaata ctgtgatata ccaaaattta gccagcaaat
    11701 atttgacaga taatagcaga ttggctgaat aaattgcggc aacaccactc cataaaaatt
    11761 gcaaaacaat cagtgtagtt atgacatgtt gaaatgcagc atttctcaat aaatcatagc
    11821 gataaaccag ataaattaca gtacacacac agagcagcat tacgataggt tcagaaggaa
    11881 tatccaagcc gaggctgcca aatgtatact caatagaaaa tggcataaca cacagcataa
    11941 tatagaatac tattcgaaaa ttgagcaacg taagtaatcc aaagagcaga gcaaagggca
    12001 atagtgcagc ttccggaatt ttataaataa aagcagttat cagcaagacc aatgacaaac
    12061 ttgcaattgt ccaaaatgcg gatgtcgcat ggcgttgaat aaaatcaatc gcgttggaat
    12121 ccgaagcggt ctgcattttt ataaagttca ataataagtg cggtaaaaca agctaaaata
    12181 aatgacccga taagcgtgga caatacgata agccaacgga tgggtttaca tttctttata
    12241 gccggagttg ggttttctac gacatacatc gtcttaaagg tattggagga cgctacagta
    12301 tattgatcgc ggatagtaat aaatttattg aggtcttcag catccgagcc atttgctttc
    12361 ttttgggcta tctgttcatc aagcaaagtg agcacatttg ttttgttgac caacagcata
    12421 tttcgataaa cagaatcagc atattgtaca atgtccttta ctacagcagc agccaacttt
    12481 ttatctctgt ccataaaagt aacttcgagt gctccattat catttcggat ggcatcaaaa
    12541 ttagaattaa attccaatgc tgtgtaatag cgatacaatc cggattcatc tttctctata
    12601 tcatagtgct tactcaggtt atatttatca ataatgtgtg tgatagcagg ctctgaagtg
    12661 agtatagtca gcatacggtt aatttcttct ttaccaccaa atggataaac acctcccgct
    12721 tctttttcat taaagagtgc tgctctgtcc gtacttacag ggttagataa ataaaatatc
    12781 attttcgact tgtaataagg aggcattatg gatggcatag tcaggataat gctgacaatg
    12841 acggcaaaca gacaaaacaa caaaatatgt tttttccatt tgtacactaa tttcacaatt
    12901 tcaatcatag aataataaac actaaaagta agtaaaaaga ttgtacgcta tggcaaattc
    12961 ttaaccgata aaatcagaat ttgccattct ttctactatt ctactacaac taatttgtcg
    13021 tcttttttac ctttcctgat gagtaaatac ttgtttttta ttaaatctgc actagagatg
    13081 attgcctgaa catcggcaaa tttttccatg ttcaagctca gtccatttcc ctgcataagt
    13141 ttgcgtgctt ctccttttga cggaaaaata gttgtttttt ctgctaataa atccaggatt
    13201 ggaattccct tttcgatatc attttgggat ataacaaaag taggtacgcc ctcgaatatt
    13261 tctaaaaagt cagtttcttc aagctccatg attttagatt tcacatcttt gtcaaacaag
    13321 atggcactca gctgttgtgc atgcaccata tctgcatggg agtgtactct ctctgtaatt
    13381 tcttcagcca gtgctttttg gagaatgcgc aggtgctcgt tgccttcatg ctgttgctct
    13441 aaggcttcta tttcttctct gttttttaat gaaaatatgc gaaaaagtgt ttttacatct
    13501 ttatcatcag ctgccagcca aaactgataa aatttatacg gagatgtttt gtttttatct
    13561 aaccagatat tgccactttc tgattttcca aactttgtgc catctgcttt ttttatcaat
    13621 tgggtagtta atgcatacgc ttcacctgcc tcttttttgc gtatcagttc agtacctgtt
    13681 acaatatttc cccattggtc gctgccaccc atttgcaaca taacattatt atttttccat
    13741 aaatggtaaa agtcatagcc ctgcaccaac tggtatgaaa attcggtaaa agacataccg
    13801 gattccaaac ggttctgaac cgaatctttg gccatcatat aatttacggt aatgtatttg
    13861 cctacatccc tgataaactg caagaatgaa aattccttga accaatcata attattaact
    13921 atctcggctg aattttctcc acaatcaaaa tctaaaaact tctccaattg tttccgctga
    13981 caggataaat tgtagttaag ttcttcttca ttcagcatat ttcgctccga actccttccg
    14041 cttggatcgc ccaccatgcc ggttgcacca cctactaatg ctatcggctt gtgtccgcaa
    14101 cgctgaaaat gcaataaggt cattacttgt accatattgc caatgcccag cgaatcggct
    14161 gtcgggtcaa aaccgatata gccttttacc atctgtttat tcagtaaatc ttcagttccg
    14221 ggcataatgt cgtgcaacat ccccctccat tttagttctt caacaaagct cttcatttta
    14281 attaaaatgt aaattgtaat aaactacaaa attattgttt accagccatt taaccatgca
    14341 acttaatttc gtttattatt atcttaatat tatcggaaaa atagtaaatt tactttgctg
    14401 aagtgtcggc aaaaactaaa tttacacatg aattgtattg gtatgattaa acaaaaagga
    14461 catatttatc tgctactggt tgtaattatt ttaggaattt acactgcaag tgccaaacct
    14521 ccgaaataca gtaatgattt tatgtatatc ggcgtaggtg caaataattt cgggctggga
    14581 aatgcggtag tggccaacac cggagatatt acctcaggat actggaatcc ggcaggctta
    14641 gtacgcatac aaaccaatct gcagtttgcc tatatgcaca atgagtattt cgccagtatt
    14701 gcaaaatatg attatgccgg tattggagta cccttagaca gtaataaaca tgcatttgcc
    14761 ataaacttta tgcgatttgg agtagatgat ataccgaata cgttagattt aatagatgct
    14821 gacggcacag tgaactatga caatgtaaaa aatttctcag ttggtaatta tgcctttttg
    14881 ctttcttatg cccgaaatat tatcaaaggc ttatctgtgg gggcgactgc aaaaatagta
    14941 cataataagg caggcggatt tgccaaatca tggggttttg gtgttgatat tggtgctata
    15001 tatcaaataa aagactggaa gataggatta accgttaaag atattaccac aactttcaat
    15061 gcatggtcgt tcagctttac agataaagag aaacaaattc tggatgccac agataatgtg
    15121 attccggtaa gttccacgga gctaacctta cccagaattc agtttggtgc atcgtataat
    15181 aagaccttta aagacaagat agaattgatg gcagaattag attgggaatt aacaactgac
    15241 ggtaagcgga atacactcat ttcttcaaaa gccatcagct tggatccccg tttgggtgta
    15301 gaactaggct tatggaaaat tggctacctg cgtggtggca tcagcaattt ccagaagatg
    15361 acaaatttga gtacaggaaa acaaaaattt acagttcaac ccagtattgg tgtaggtctg
    15421 cgctataaaa atatcgttta tctggattat gcatttacgg atgtaggcaa aaccagtgaa
    15481 aatcaatact ctcatgtcat ttcattaaaa gtggggataa acaaacgtaa gcataaagaa
    15541 aatgctgaac tgattaatgc tgcaccgcaa gagaatgtaa gcggacaaga acaaccggcc
    15601 agtcagccaa cagaaattca aagtccggaa aaagaaaata ataataacgc tcaacctaaa
    15661 taatgaaaaa attactcgct atagtatttc tcatcacatt atgccgtagt ttgggctttg
    15721 ctcaaccata cggaaacgag tggatagatt ataacaagac ttactataaa tttaaaattg
    15781 cccaagacgg tttgtgcagg ataaattatg cgacacttat cgccacaggc atacctgctg
    15841 ctcaattaaa aggcactgat ttcaaattga tatgtaatgg tgaggagata cccatttatg
    15901 taactacagc caatcaattt acagcaagtg attttataga attttatggt gaaaaaaatg
    15961 acgggagtgt agacacccgc ttatataaaa atccactaga gca
//
LOCUS       DAOQII010000026        15416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1822_length_15416_cov_5.903001, whole genome shotgun sequence.
ACCESSION   DAOQII010000026 DAOQII010000000
VERSION     DAOQII010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1822_length_15416_cov_5.903001"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1518)
                     /locus_tag="PLF48_02400"
     CDS             complement(235..1518)
                     /locus_tag="PLF48_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNF48202.1"
                     /translation="MKLFKQLCVLSLSFILFSCNNDGKKTGDTSGFEKDEKFYKEFQE
                     KLILVEDGGTVEFPSGKYLLSKPLSMEGKKNITIKGAGSEKTVLSFLDQTDGADGMLI
                     TNCQNVTITGFTIQDAKGNNLKLKGCDGVHIHHMNSTWTTGADTANGNYGYYPVECKN
                     VLMEYNEVSYCADAGVYIGQSTNVEIHHCYAHHNVAGIEIENCINSDIYQNKAENNAG
                     GILIFDLPKLFQANGRNAKVWDNDCINNNFKNFGKPGSIVSMIPPGSGMIIMATDSVD
                     VFNNRIINSKTVGIAVVSYFITQKKINDSTYGPYCQSVYIHDNKIDRSGLSLKEKVPD
                     LTTDLGKLLTGAFKGAVDILVDGSADPKIRDANGKLPESKRICIRNNGSNIIFGNLNM
                     WKASGIKDILSYKDNDITAFDCSYTGINGNGKKAE"
     gene            complement(1592..2482)
                     /locus_tag="PLF48_02405"
     CDS             complement(1592..2482)
                     /locus_tag="PLF48_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNF48203.1"
                     /translation="MSVIVIAGGTGMMGKCISKALTNAGNEVRILARNPKLDNEYRWD
                     IETGYIDTAVLENADVIINLAGANIGTHRWTTAYKKEILFSRVQSSQLIYDTLLTHKH
                     NISRYISASAIGYYGHRPGEILKETASNGNGFLAKVCSEWEKAALITASLNIPVAILR
                     IGVVLSKEEGALPKMSIGNQFGLGVILGNGQQYISWIHIDDLVNIFTFLISNKFNGIF
                     NAIAPLPVTYEKLSEAISISKQSKHIPIKIPEFAVKLLLGEQSEMVLSDTYCRAEKIL
                     HTGFAFQYPDIHVALRQLYT"
     gene            2964..4727
                     /locus_tag="PLF48_02410"
     CDS             2964..4727
                     /locus_tag="PLF48_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF48204.1"
                     /translation="MRIKKTIYVLLGVILLLLLLASLNLFNHKKVYKNEVVVHVLSDP
                     KGLNPLTVSDATTRNYISPYVFQPLIAYDFKTYNMLPVLAVAVPEVIRNGNELTATLE
                     IRPEARWDDGTPVTANDVAFSFKSIMCPNAQTANLRPGVDFFKEIILYPDNPRKITFR
                     TGLILDLLSSFYEIKILPENVFDPKHILRKYSFSQLITDADRLSTAKDIKEFTDLFNS
                     DKAAHDIDMAKGSAAYKAVAFETGQRVILEKKKNWWGDKLVDVNMYFEAFPKRLVFET
                     INDFNTAFTALKNEQIDFLFSTPIKDYIDLDNSPKFKKNFIKSNPPMLGYQGIGMNYA
                     DPILKDTVVRRALAHLINVDQLIEKVFYGFAQRTIGSIQPGKPYYNNNIKPYTYNPEL
                     AMMMLAERGWKDSDNDGILDKVIDGKKTDFEVTFTYNSGNSVREMVGLLFQRACYIAG
                     IKVNVKPMDWSLFLDDLKKHKCQMWYQGWVSNPGLDDDKQIYYTSSAIDGGNYMNFGN
                     AKTDKIIDDIRVEMDSAKRNAMYMEWQEIQNADLPYIYLYIQTFRNCVHKRFENIHET
                     SIYPGAWFAGFKVKKEYKVED"
     gene            4742..6163
                     /locus_tag="PLF48_02415"
     CDS             4742..6163
                     /locus_tag="PLF48_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF48205.1"
                     /translation="MLKYIVKRILIFIPTLIVISLATFYLSVHVPGDPVEQMLNSGSD
                     AGSSANSLASEKAYIDKRKELGLDLPIFYFEVTNAAKPKYLYEVAKKFHRDNLGVLAE
                     RYGNWDKIAPYYESIKTFNLRAADVPKDSANGDILIGIKSNIQKLFLENDEQILKTTM
                     ADIQTELDSNTQVRTALQPAFDSTRNAYQAVLKTQQPNKHLIPAFYWYGTNNQYHRWI
                     AKFIRGDFGISYQDGRPVKEVIWSALRWTLILSFISILLTYLIAVPLGITSAANKGSV
                     KDQSITTSLFVLYSLPNFWVATLLIMFLGGGDFLDIFPANGVGQITEGMSFFEKLWTR
                     AHHLVLPIICFTYGSLAFLSRQMRGAMINTLGQDYIRTARAKGLDEDTVLWKHALKNS
                     LLPIITLFANVFPLLISGALVLEFKFTIPGMGKTAYEALLARNYPIVYTIVMFSAILT
                     LVGFLVADILYAVVDPRIKFDKK"
     gene            6166..7395
                     /locus_tag="PLF48_02420"
     CDS             6166..7395
                     /locus_tag="PLF48_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF48206.1"
                     /translation="MAEELKQDYWSLVKRQFKKNKRALYSLYVVGFMILIALLADFLA
                     NEKPIYCKLDGKSYFPIFNEYGVKLKLAKYPPELNNIVWLDKKFDAVVRAPIPYSPGT
                     QDILNFGFVSPFGKQDVKSLRWRHWLGTEALGRDILSALIHGTRIALTVGLVSMTIAS
                     IIGILLGSLAGFFGDNRLRISRATIFMAFIFGFFGFFYAFAFGKVFLGLIIITASFFL
                     AYLAGFALKPIPFFKKKMAMPMDIIVQRLIEVLVSLPAMFLIIAIVAISKPSIYLVMA
                     VIGCISWTGIARFIRAELLKVRSLEYIEASEALGYPQWRILLKHAIPNAISPVLITIA
                     FGIASAILTESFLSFLGVGIPPETLTWGKLLSVAGGGRSQLWVAIFPGIAIFLTVTLF
                     NLVGEGLTDALDPRQKK"
     gene            7420..>8885
                     /locus_tag="PLF48_02425"
     CDS             7420..>8885
                     /locus_tag="PLF48_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48207.1"
                     /translation="MKKKKFYIRLTIFHKTIWSIIGIVIFSYELSAQTIIKSTPEKVS
                     SAYSDYELIGNSPAGILVHYFGQNQSELISYDDNLKVKNRRELPYKGKDFKIESFLPT
                     EHNLWLFYTLKENGYLYLRAKQMDNNLNIQNDYYPLDSISNAAIGNEGKTFYVKTSPD
                     KSKILLFGVIRSRASLFVRFTVLNDSLRILSKNIFSFSDGKNPMLKSVRVNNNGYVTG
                     VVGDVFNNFNTSDYDMERYTVYTYNPETKTIGEIPINDKDFAYKNLITETSNSRDIFY
                     ISAAYKNEQDKNDIGYIMKTIDFRTNNVLVSNTIKFTKDILQKTQTYDFNSWQDKASL
                     FKPKRIIPRSDGGMLLITEAEYKFTKVDRNSISNNGSYGVTPYAYSVRYVDQFQFYDM
                     MSYSINKEGVIEWQSTMPKSQISEEDDAMYSSFAFFESNTLLKFLYHEDYYNSGNFIE
                     YNLNPNGQSKRVSLFNSDKENIAVVPKKGKQIDGFTML"
     assembly_gap    8886..8985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8986..10272)
                     /gene="odhB"
                     /locus_tag="PLF48_02430"
     CDS             complement(8986..10272)
                     /gene="odhB"
                     /locus_tag="PLF48_02430"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNF48208.1"
                     /translation="MSKVEIKVPAVGESVSEVTLAAWLVKEGDFVKLDQPLAEFESDK
                     ATFELPAEVAGTITFNAQEGDDIKIGGVVAFIDTDGTQSSVNSQQSAVGKTEEKNEKE
                     NNKLSSENQQENYAAGTPSPAAKKILDEKGIEASEVSGTGKDGRITKEDAEKVEKPIV
                     DSRQSTENTSTEVTETIDRQTSNNSFSRIERIEKLSRMRKTISRRLVAVKNETAMLTT
                     FNEVDMTRIHQIREQYKKPFEEKNGIGLGFMSFFTKACALALQKFPAINAYIDDENIT
                     YHDYCDISIAVSTPKGLVVPVIRNAESLSFAGIEKEVKRLALLGRDGALSMQDMEGGT
                     FTITNGGVFGSLMSTPIINQPQSAILGMHKIQDRPMAINGKVEIRPMMYLALSYDHRI
                     VDGKESVGFLVMVKDLLENPEKMLYGSDPVKVLLEL"
     gene            10563..11147
                     /locus_tag="PLF48_02435"
     CDS             10563..11147
                     /locus_tag="PLF48_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48209.1"
                     /translation="MKNAIIAILALLCGFLGWKLYALNHQEQVEKHLDPNNNKDLFTC
                     ASYSFANSPFPDVLDSTGKINNEYVLTNNDEFNYNTRTNRRTTDGKLEDTKLLMETEM
                     LKYIYDYATMSNKKYIIFYYLETENYTERKNYFFDRHSSIHAYRETSRWRQSIKDATY
                     LVADLPVSRADFSKATTSKIEVGKLCPPPGGSCE"
     gene            11176..11661
                     /locus_tag="PLF48_02440"
     CDS             11176..11661
                     /locus_tag="PLF48_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48210.1"
                     /translation="MKNAITAVLALLCGFLGWKLYKNSKPVYNLMELQSTLFKTLTST
                     DRRADSCVLAFSKNLVKDDPQKKNIRGIRLSALDLASLNAYVEISKSDAVYFSLSQIV
                     DKDIAAEIVTANIKDDPTMTIDKVMGRPFINIFVQKSGNPTLTCKSTYKVCPPPNGVC
                     P"
     gene            complement(11658..13448)
                     /locus_tag="PLF48_02445"
     CDS             complement(11658..13448)
                     /locus_tag="PLF48_02445"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF48211.1"
                     /translation="MKNYFSLYFILLTLLFFVACSSETIAPNEPTYTEIEALIIQADS
                     AFVMENSNYADSIIQIALQKAEATRNADIILKTLFYNTSDNISINSTVERLDKMGKFI
                     NKGLDYAKMFKRLDYIAYGNANLAEWYLAKGDLNQAVAAANLAFSTALGIQNDSCKIV
                     CALQLGRVYERQKNTLLAFRTYTNAHDIANKSGNEKYIPMVYLKLGGLYESLNKNEEA
                     KDYYFRSLELDKKLKDIDGQIASNVALGDIYEYRMAKDYYETAEKQALQYKRMRLLKI
                     VQLHYFSYKIINGIPNDALRYYQQQPMIEEIYKNRGDKYVNWILAEIHLYGSQPDTAK
                     FYFDKCTEAFQSDYDKNARLSLLMEYGMALTQLKKNTEATAVYRDAFELSTQTSNSSY
                     AINSAEELSRLYEQNSNYKEALLFAQHAENYKDSVQALSKERDFASLEIENENKRIEN
                     EKLKELEAEHHRHNIQYLAIIIAITFVFLLLMISGIMTISSRAIEILGFFSFIFLFEF
                     IIMLLDHKIIALTHGEPLKIWLIKIALISLLMPFHHFIEEGLIHYLTSKKIIKVKERL
                     NLKKLIPRKKAITVTNEDASTTDNAVIDEH"
     gene            complement(13579..14397)
                     /gene="panB"
                     /locus_tag="PLF48_02450"
     CDS             complement(13579..14397)
                     /gene="panB"
                     /locus_tag="PLF48_02450"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNF48212.1"
                     /translation="MSAATNEIKRVTTHKMKEMKQRGEKISMLTAYDFSMAKILDDAG
                     IDILLVGDSASNVMAGHETTLPITLDQMIYHAQSVVRAIKRCFVVVDLPFGSYQGNSL
                     EALNAAIRVMKETGGHGVKLEGGQEIGESIIRILTAGIPVMGHLGLTPQSIYKFGTYS
                     VRAKAEDEAEQLIEDAKYLESIGCFALVLEKIPAHLAEKVAKTISIPVIGIGAGGGVD
                     GQVLVTHDMLGITHEFKPRFLRRYLELYDQIKEATTQYISDVKSNDFPNEKEQY"
     gene            14518..15354
                     /locus_tag="PLF48_02455"
     CDS             14518..15354
                     /locus_tag="PLF48_02455"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNF48213.1"
                     /translation="MKFLNTMTDAELVEFGKKNHDAYINAQPFPSGYYDNLFNPELLR
                     EIIKEFPVIGKEKEDIQYSNPNEVKLATKGEYRFGPNTKAFVHYLNSQPFLEFLQNLT
                     GIKETLIPDPYFEGGGFHEIKPNGYLKMHVDFHKHKLTNLDRRLNILVYLNEDWKDEY
                     GGHFELWERDMSKCVTKVAPLFNRLAMFSTTDFSWHGLPDPLTCPPDRSRRSIALYFY
                     TNGRPEGEVNTGDKARITTTFATRDGQDSGKMKAFNGFVNLANDLLPPIIVKTIKKFR
                     NT"
BASE COUNT         4720 a         2881 c         2834 g         4881 t
ORIGIN      
        1 tactccgata atttttcaaa aggcttggca tttatatcta ccgttacttt tccggaagaa
       61 tttgttgttt tacattgaat aaacagcaaa acaaaagcac atagtaaaag gattatcttc
      121 aggtgctgct tactaatcag tttcattgtc aatcattttt attaatatta tctacttgaa
      181 tcaatattaa atgtgattca gttttttaaa atctcagcag tataaatcaa aagactactc
      241 tgcttttttt ccgtttccgt ttattccggt atagctgcaa tcgaaagcgg tgatatcatt
      301 gtccttataa cttaaaatat cttttatgcc actagccttc cacatattta gattgccaaa
      361 aataatattg ctaccattat tgcggataca aattcgcttg ctttcaggaa gctttccatt
      421 cgcatctctg atttttggat ctgcgctgcc atctaccaaa atatctaccg cacctttaaa
      481 tgctcctgtc aacagcttcc caaggtctgt tgtcaagtcg ggtacttttt ctttgagcga
      541 caagccgctt cggtctattt tattgtcatg tatatacaca gactggcaat acggaccata
      601 tgttgaatca ttaattttct tttgtgtaat aaaataactt actacggcaa tgccaaccgt
      661 tttactgttg atgatcctgt tattgaatac atccacactg tctgttgcca taataatcat
      721 accagagccg ggaggaatca tggaaacgat gcttccgggc ttgccaaagt tcttaaagtt
      781 attattgata cagtcattat cccatacttt tgcatttcgt ccattagcct gaaataattt
      841 aggcaaatca aaaatcaaaa taccaccggc attgttttct gccttgtttt gataaatatc
      901 cgaattgatg cagttttcta tttcaatacc tgctacattg tgatgcgcat agcaatgatg
      961 aatttctaca tttgtagatt gcccgatata cacacctgca tctgcacaat aggatacttc
     1021 gttatattcc atcaatacat ttttgcattc aaccggataa tagccatagt tgccgttagc
     1081 agtatctgct ccggtagtcc aggtagaatt catatggtgg atatgcacac catcacaacc
     1141 tttcaatttt aagttgttcc cttttgcatc ctgaatcgta aaacctgtaa tggttacatt
     1201 ctggcagttg gtaatcagca tgccgtcagc accatctgtt tgatctaaaa atgacaagac
     1261 tgttttttcg ctgccggccc ctttgatggt aatatttttt ttgccttcca tagaaagcgg
     1321 ttttgataac agatattttc cggatggaaa ttcgacagta ccaccgtctt caaccaaaat
     1381 taatttctcc tgaaattctt tatagaactt ttcatctttt tcaaaaccgg aagtatctcc
     1441 agttttttta ccatcattat tacaggagaa caagataaaa gataaagata aaacacagag
     1501 ctgcttaaat aatttcatga ctacagatat tttagtgaaa atagtgaatt aatgacagtt
     1561 atgcaagctt aaatattgaa gttcaccttt gttaagtgta taattggcga agtgcaacat
     1621 gaatgtcggg atattgaaat gcgaaaccgg tatgcaagat tttttcagcc ctgcaatacg
     1681 tatcggataa taccatttcc gactgctctc caagcaacag ttttactgca aattccggaa
     1741 ttttaatggg aatatgtttg ctttgtttgc ttatagaaat ggcttctgat aatttctcgt
     1801 aagtaaccgg aagcggtgca atcgcattaa atataccatt aaatttatta gatattaaaa
     1861 atgtgaatat atttactaaa tcatcaatgt gtatccatga aatatattgt tgaccattac
     1921 ccaaaatgac accaagtcca aattgattac caatggacat tttgggcaat gcaccttctt
     1981 ctttactcaa aaccacaccg atacgcaata ttgctaccgg aatgtttaaa gatgcagtta
     2041 ttaaggcagc cttttcccat tcactgcata cttttgccaa aaaaccattc ccgttgctgg
     2101 ctgtttcttt gaggatttca ccggggcgat gtccgtagta gccaatagcc gaagcagaga
     2161 tgtatcgaga aatgttatgc ttatgcgtaa gcaatgtatc ataaattaat tgggatgatt
     2221 gaactctgct aaataatatt tcttttttat acgcagtagt ccatcgatgt gtgccaatat
     2281 ttgctcctgc caaattgata attacatctg cattttctaa gacggcagta tcaatatatc
     2341 cggtttcaat atcccatctg tactcgttat ccaactttgg atttctggcc agtattcgca
     2401 cctcgttgcc tgcattagtc aaggcttttg aaatgcattt tcccatcata ccggttccac
     2461 cggctataac aatgactgac atcgtgtgaa tttagaatta aacgataaaa caaacaaata
     2521 aaatactaat agaaaagcaa ggttttcaca ccaactcagc aattgacggt ggcaatttta
     2581 tgaattttgg ctgtgccaga actgatgagt tgattgagaa gataagaatt gaaccggatg
     2641 ttgcaaaaca caataactat atataatgca gtggcaggag atagggcaca atgagatttc
     2701 atttatatat atctttgtac aacaatttag aaactgtatc aataatagat tttagaattt
     2761 gcacgaaaca tctatgatac ccggtgcttg gtttgcagaa tttaaagtaa aaaaaggata
     2821 tactattgaa gccgataagt gaaacatccg tcaatatgaa attgaatagc aatccgcata
     2881 ggccataatt ataataaaaa atttagccat acacgaaata taaaacggaa atagtacatt
     2941 tgtagatatt caacagtttt gctatgcgca taaagaagac gatatacgtt ttattggggg
     3001 ttattttgct cttgctttta ctcgcttctt taaatttatt taatcataag aaagtttata
     3061 aaaacgaagt agttgtccat gttttatccg acccaaaagg actaaacccg cttaccgtat
     3121 cggatgctac tacccgaaat tatatttctc cttatgtatt tcagccgctc atagcttacg
     3181 atttcaaaac gtataatatg ctgccggtgt tggcagttgc agtacccgaa gttatccgca
     3241 acggtaatga actgacggct actttagaga ttcgccctga ggcgcgctgg gacgacggca
     3301 ctcctgttac tgccaacgat gtcgcctttt catttaaatc gataatgtgt cctaatgcac
     3361 aaacggctaa cctcagaccg ggtgtagatt tttttaaaga aataatttta tatcctgata
     3421 atcctcgtaa gattactttc cgtacaggat taattctcga tttgttgagt tcattttacg
     3481 aaataaaaat actacccgaa aatgtattcg accctaaaca tatattgaga aaatactctt
     3541 tctctcaatt aattaccgat gccgatcgtt taagtacggc aaaagacata aaagagttta
     3601 ccgacttatt taattcagac aaagcggctc atgatattga tatggctaaa ggctctgcag
     3661 catacaaagc tgttgcgttt gaaacaggac agcgtgtaat tcttgaaaaa aagaaaaact
     3721 ggtggggcga taagctggtc gatgtcaata tgtatttcga agcatttccg aaacgtttgg
     3781 tttttgaaac gatcaacgac ttcaataccg cttttacggc tctgaaaaat gaacagatag
     3841 actttttatt ttctacacct ataaaggatt atattgattt agataactct ccaaaattta
     3901 aaaagaactt tataaaatcg aatccgccga tgttaggtta tcagggtata ggaatgaatt
     3961 atgccgatcc aattttgaaa gatactgttg taagacgggc attggcgcac ttaataaatg
     4021 ttgatcaatt aattgaaaaa gtattttacg gttttgctca gcgtacgatt ggctctattc
     4081 agccgggaaa accatattac aacaacaaca tcaaaccgta tacatacaac cccgaacttg
     4141 ctatgatgat gctggcggaa agaggctgga aggattcgga taatgatggt atattagaca
     4201 aagtcataga tggcaaaaaa acggattttg aagttacctt tacttacaat agcggaaatt
     4261 cggtacgcga gatggtgggt ttgttattcc aacgggcttg ttatattgcc ggcattaagg
     4321 taaatgtaaa acccatggac tggtcattgt ttttagatga cttgaaaaaa cataaatgcc
     4381 agatgtggta ccagggctgg gtgagtaatc ccggtttaga cgacgataaa caaatatatt
     4441 atacttcgag tgcaattgat ggcggaaatt atatgaattt tggcaatgct aaaaccgata
     4501 agattattga tgatatacgg gtagagatgg actctgctaa gcgaaatgcg atgtatatgg
     4561 agtggcagga aatacaaaat gccgatttgc cctatattta cttgtacatc cagacattca
     4621 gaaactgtgt tcataaacga tttgaaaaca tacacgaaac atccatctat ccgggtgcat
     4681 ggtttgccgg atttaaagta aagaaagaat acaaagtaga agattaacag aagaaataca
     4741 catgttaaaa tatatagtca aacgcatcct gatttttatc ccgacactga tcgtgatttc
     4801 tttggctacc ttttatttga gtgtgcatgt gcccggtgat cccgtagagc agatgctcaa
     4861 ctcaggaagt gatgccggaa gtagtgctaa ttcgctagca tccgaaaaag cctatattga
     4921 caaacgtaaa gaactcgggc tggatttgcc tattttttat tttgaagtga cgaatgccgc
     4981 taaaccaaaa tatttatatg aggtggcaaa aaaatttcac cgggataatc tgggagtatt
     5041 agctgaacga tatggcaact gggataaaat agcaccttat tacgaaagta taaaaacttt
     5101 taatctcaga gctgcagatg ttcctaagga ttcggctaat ggagatatat taatcggtat
     5161 aaaatccaat atacaaaaat tgtttttgga aaatgatgag caaatactga aaacaacgat
     5221 ggcagatatt caaacagaac tggattctaa tactcaggta agaacagcct tgcagccagc
     5281 ttttgattct acccgaaatg cttatcaggc agtattaaaa acacaacaac cgaacaaaca
     5341 ccttatccct gcattttact ggtatggtac taataaccaa taccatcgct ggatagctaa
     5401 gtttattcgc ggcgatttcg gaatctctta tcaagatggt cgtcctgtaa aagaagtaat
     5461 atggtcggct ttgcgctgga ccttaattct gagttttatc tctattttat tgacttactt
     5521 aattgccgta cccttgggta ttacttcagc ggcaaacaaa ggcagtgtga aagatcaatc
     5581 gatcactaca tcattattcg tgttatactc tctgcccaac ttctgggtag ctacattatt
     5641 aatcatgttt ttaggcggtg gtgatttcct ggatattttt ccggcaaatg gtgttggaca
     5701 aattacagag gggatgtcat tctttgaaaa gttatggaca cgcgcccatc atcttgtttt
     5761 acctattatt tgttttactt atggaagttt ggctttctta tcaagacaga tgcgtggcgc
     5821 aatgattaat acacttggtc aggattatat tcgaactgca agagctaaag gcttggatga
     5881 agatactgtg ttatggaaac atgccctgaa aaattcactt ctgcctatca ttactctctt
     5941 tgccaacgta tttccattgc tgataagcgg tgcattagta ctggaattta agtttacgat
     6001 tccgggcatg ggaaaaacag cttatgaagc actactagcc agaaattatc ctattgtcta
     6061 taccatcgta atgttttctg cgatacttac actggtagga tttttagtag ccgatatatt
     6121 atatgcagtt gtagatccac gtattaaatt cgataaaaaa tagaaatggc agaagaatta
     6181 aagcaagatt actggtcgct ggtcaaaaga cagtttaaaa aaaataaacg agctttgtat
     6241 tcgctgtatg ttgttggctt tatgatttta atagccttgc tggcagattt cttagccaac
     6301 gaaaagccta tttattgtaa gttagacggc aaatcttatt ttccgatttt taatgaatat
     6361 ggtgtaaaac taaagcttgc aaaatatccg cccgagttga ataatattgt ttggctggat
     6421 aaaaaatttg atgccgtagt gcgggctccc attccttatt cgccgggcac acaagatatc
     6481 ttgaatttcg gtttcgtatc cccgtttggc aagcaagatg tgaaaagttt gagatggcgg
     6541 cactggctgg gtactgaagc cttgggaaga gatattttat ctgcactgat acacggaaca
     6601 agaattgcat tgacagtagg cttggtatct atgactattg ccagtatcat cggtatactt
     6661 ttgggttctt tagccggttt ttttggtgac aaccgattgc gcatttcgcg tgctaccatc
     6721 ttcatggctt ttatctttgg tttctttggt ttcttttatg catttgcttt cggaaaggtg
     6781 tttttaggac ttatcattat aacagcatct ttctttttgg cctatttggc agggtttgca
     6841 ctaaaaccta ttcctttttt caaaaagaaa atggcaatgc ctatggatat tattgttcaa
     6901 cgattgattg aggtactggt ttcactacct gcgatgtttt taattatagc catagtagct
     6961 atttccaaac ccagtattta tttggtcatg gcggttattg gctgcatttc ctggacaggg
     7021 atcgcacggt ttatccgtgc ggaattgctt aaagtaagaa gtttggaata tattgaagcc
     7081 tcagaagcat taggttatcc gcaatggcgt attttgctga agcacgctat ccccaacgcc
     7141 atttctccgg tattaatcac tattgccttt ggcatagcat ccgctattct tacagagtcc
     7201 tttctgtctt tcttaggagt gggcattccg cccgaaacac ttacatgggg aaaactgctt
     7261 tcagtagctg gcggaggccg ctcacaactt tgggtggcta tatttccggg tattgccata
     7321 tttttgactg taacattatt taacctcgta ggtgagggat taacagatgc attagaccca
     7381 cgacaaaaga aataatgcgc atatttgtat catatacaaa tgaaaaagaa gaaattttat
     7441 atccgcctta ccattttcca taagacaata tggagtataa ttggtattgt tattttctca
     7501 tatgagcttt cggcacaaac catcattaaa tccacacccg agaaagtgtc tagtgcttat
     7561 tccgattacg aactgattgg caatagccct gcgggtattc tcgtgcatta tttcgggcaa
     7621 aaccaaagcg agctgataag ttatgatgat aatctgaaag taaagaaccg ccgcgaactg
     7681 ccatataaag gaaaagactt caagattgaa agtttcttac cgaccgaaca taatttatgg
     7741 ctattttata cactgaaaga aaatggttat ctgtatttac gagcaaaaca aatggataat
     7801 aacctcaata tccagaatga ttattatccg ttagattcga tttctaatgc tgccattggc
     7861 aatgagggaa aaacgtttta tgtgaaaaca tctccggata aatctaaaat attgctgttt
     7921 ggcgttatcc gctcaagggc ttcattgttt gtgcgattta ccgttttgaa tgattcattg
     7981 cgcattttat caaaaaatat tttttcattt tctgacggga agaacccgat gctgaaatct
     8041 gtacgggtaa acaataacgg ctatgtgacg ggtgtagtag gtgatgtatt taataatttc
     8101 aatacttcag attatgatat ggaacgctat acggtgtaca cttataatcc ggaaacaaaa
     8161 accataggag agattcctat taatgataag gattttgctt ataaaaatct gataacagag
     8221 acgtcaaaca gtcgtgatat tttttatatc agtgcggcat acaaaaacga gcaggataaa
     8281 aatgatattg gctatatcat gaagacgata gatttcagaa cgaataatgt gctcgtgtct
     8341 aacacgatta aattcactaa agatattttg cagaaaacac aaacgtatga ttttaacagc
     8401 tggcaagata aagcatcttt attcaaaccc aaacgcatta ttccacgcag tgatggcggc
     8461 atgttgctaa ttacagaggc ggaatacaaa tttaccaaag ttgacagaaa ttcaatatcg
     8521 aataatggaa gttatggcgt tacgccatat gcctattcag ttcgctatgt cgaccaattt
     8581 cagttttatg acatgatgtc ttactccatc aataaagaag gggtaattga gtggcaaagc
     8641 acgatgccca agtcacaaat ttctgaagaa gatgatgcca tgtattcttc cttcgctttc
     8701 tttgaatcca ataccctgct caagttttta taccacgaag attattacaa ttccggaaat
     8761 tttatagaat acaacttgaa tccaaatgga caaagtaagc gggtgagctt atttaattca
     8821 gacaaagaaa acatagctgt cgtacccaaa aaaggcaaac agatagatgg ttttaccatg
     8881 ttattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttata gctccaacaa
     9001 aactttcacc gggtcgctgc cataaagcat tttttccgga ttttctaaaa ggtcttttac
     9061 catgactaaa aacccgacgc tttctttgcc atccacaatt cgatgatcat agctcaatgc
     9121 cagatacatc atcggtctta tttctacttt gccatttata gccattgggc ggtcttgtat
     9181 tttatgcatc cccaaaatgg cactttgcgg ttggttgata ataggtgtac tcatcaagga
     9241 gccgaatacg ccaccattgg taatggtaaa cgtaccgcct tccatgtctt gcatactgag
     9301 tgcaccatct ctgccgagca gagcaaggcg ttttacttcc ttctcaatac cggcaaatga
     9361 taaactttcg gcattgcgta tcaccggaac taccaaacct ttgggtgtag atacggcaat
     9421 tgaaatatcg caataatcgt gataggtaat attttcatcg tcgatatagg cattgatagc
     9481 cggaaatttt tgcagtgcaa gcgcacaagc ttttgtaaag aacgacataa agcccaaacc
     9541 aataccattc ttttcttcga atggcttttt atattgctct ctgatttgat gaatgcgtgt
     9601 catatccact tcattaaaag tagtgagcat ggcagtttca tttttcaccg ccaccaaacg
     9661 acgcgaaatc gttttgcgca tccgcgaaag tttctcgatt cgctcaatgc gtgagaagct
     9721 attattcgat gtttgtctgt cgatagtttc tgttacttca gttgaggtat tttctgtcga
     9781 ctgtcgactg tcaactattg gcttctcaac tttttccgca tcttctttgg taattctgcc
     9841 gtctttgcct gtacccgaca cttcagaagc ttctattcct ttttcatcta atattttttt
     9901 tgctgccgga gatggcgttc ctgctgcata gttttcttgt tggttttcgg atgatagttt
     9961 attgttctct ttttcatttt tttcttcagt cttgccaact gctgactgct gactgttaac
    10021 cgacgactgt gttccatcgg tatcaataaa cgccaccaca ccgccaattt taatatcatc
    10081 accttcttgg gcattaaagg taatagtgcc tgctacctca gccggaagtt caaaggtggc
    10141 tttatccgat tcaaactcag caagcggctg atctaacttc acaaaatcgc cttctttcac
    10201 caaccaggcg gcaagtgtta cttcgctaac actttcccct acggcaggaa cttttatttc
    10261 tactttgctc atagtacaag ttatttattt ggatgcgaac aaagttacga tttctgaatg
    10321 gaataaaaag aggagtattt aatggcaaaa tttaataaga atgagaatat ttgaattgtt
    10381 ttaaatgatg aatattgcaa aattctaaaa ggtgggttaa agaagataca cccattcatc
    10441 catcacaaat tggaaaaaaa tccaaagatc ttacaaagaa aacttgcttt acgtattgta
    10501 agtattaaat aattttggta tattagtatc tgaaaatcat ttttaaccaa taaaacattt
    10561 ttatgaaaaa cgcaattatc gccatactgg cacttttatg tggctttttg ggatggaaat
    10621 tatatgcact aaatcatcag gagcaagtag agaaacattt agaccccaac aacaataaag
    10681 atctttttac ttgtgcaagt tattcgtttg cgaattctcc attccctgat gtattagaca
    10741 gtactgggaa aataaataat gaatatgtat taactaacaa tgatgaattt aactacaata
    10801 ctaggacaaa tagaagaaca acagatggca aactagaaga cacaaaatta ttaatggaga
    10861 cggaaatgct aaaatatatt tatgattatg ctacaatgag taataaaaag tatattatat
    10921 tttactattt agaaacagaa aactacacag aaagaaagaa ttattttttt gatagacatt
    10981 cttctattca tgcatatagg gagacaagta gatggagaca atctattaaa gatgctacgt
    11041 atttagttgc tgacttacct gtatctcggg ctgatttctc taaagcaact acttctaaga
    11101 ttgaagtggg aaaattatgc ccaccacctg gtggatcatg tgaatagttt tttattcata
    11161 attataacaa atattatgaa gaacgcaatt accgccgtgc tggcacttct atgtggcttt
    11221 ttgggatgga aattatataa aaacagtaaa ccagtatata atttgatgga actacaatct
    11281 acactattta aaactcttac aagtaccgac aggagggcag attcatgcgt gttagcattc
    11341 agtaagaatc tcgttaagga tgaccctcaa aaaaagaata ttaggggtat tagactttct
    11401 gctttagatt tagcatcgct caatgcatac gttgagattt caaaatcgga tgctgtttat
    11461 tttagccttt cacaaatcgt agataaagat attgctgcgg agatagtaac agctaatatt
    11521 aaggacgacc ctactatgac tattgacaaa gttatgggaa gaccctttat aaatatcttt
    11581 gtacaaaaaa gtgggaatcc aacgcttact tgcaaaagta cgtataaagt ttgtcctcct
    11641 cctaatggtg tttgccctta gtgttcgtct ataactgcat tgtcagttgt agatgcatct
    11701 tcatttgtaa ctgtaattgc ttttttgcgg ggtattaatt ttttgagatt tagacgctct
    11761 tttactttta ttattttttt ggatgtgagg tagtgtatca atccttcttc aatgaaatga
    11821 tggaatggca taagcaatga aatcaaggca atctttatca gccatatttt caaaggttca
    11881 ccatgtgtaa gcgctattat tttatggtcg agcagcataa tgatgaactc aaacaagaag
    11941 ataaaagaaa agaaaccgag tatttcaatt gcgcgcgatg aaatggtcat aataccgctt
    12001 atcatcaata ataaaaacac gaaggtaatg gcaataatga tagccaaata ttgaatattg
    12061 tgccggtgat gctccgcttc aagttctttg agtttttcat tttctatacg cttgttttca
    12121 ttctcgattt ccagcgaagc aaaatctctt tctttagata atgcctgtac agaatcttta
    12181 taattctctg catgttgggc aaacaacaat gcttctttat agttactgtt ttgttcgtat
    12241 agtctactga gctcttctgc gctattaatg gcataggagg aattggaagt ttgtgtgctt
    12301 aattcaaatg catctctata tacagctgtc gcctccgtat tcttttttag ttgagtcagc
    12361 gccatcccgt attccatcaa aagactcaaa cgcgcgtttt tatcataatc actttgaaat
    12421 gcctccgtac acttgtcaaa ataaaacttg gcagtatcgg gctggctgcc atatagatga
    12481 atttcagcca gtatccagtt gacatattta tctcctcggt tcttgtaaat ctcttctatc
    12541 attggttgct gctgataata cctaagtgca tcgttgggaa tgccgttgat gattttataa
    12601 gaaaaataat gcaactgaac aatctttaat aaacgcatcc gtttatattg caatgcctgt
    12661 ttttcggctg tttcataata gtctttcgcc attcggtatt cgtaaatatc gccgagcgcg
    12721 acattagaag caatctgtcc gtcaatatct tttaatttct tatccagctc cagacttctg
    12781 aaataataat ctttagcttc ttcattcttg tttaagctct catataaacc gcccaactta
    12841 agatacacca taggaatgta tttttcgttt ccgcttttgt tcgcaatatc atgtgcattg
    12901 gtataagtgc ggaaagccag cagcgtgttt ttttgccttt catagactct tcccaattgc
    12961 agggcgcata caattttaca ggagtcattt tggatgccta atgctgtgga gaatgccaga
    13021 tttgcagcag ctaccgcctg attcaaatca ccttttgcca gataccactc tgctaagttg
    13081 gcgttgccat aagcaatata atccagccgt ttgaacattt tggcgtagtc caatccttta
    13141 ttaataaatt ttcccatttt atccagccgc tctacggtag aattgatact gatgttatca
    13201 gatgtattat aaaaaagtgt tttaagaatg atgtctgcat tacgggtagc ttccgctttc
    13261 tgtagggcaa tctgaataat gctgtctgca tagttgctgt tttccatcac aaaggcagaa
    13321 tcggcttgaa ttattagagc ttcaatttcg gtataggttg gttcattagg tgctatcgtt
    13381 tctgagctgc aagctacaaa gaacagaagt gttaataata taaaatataa actaaaatag
    13441 tttttcataa gcgcatggtt tattacgcaa ataaatcaat cactgtgaat atacaaaagt
    13501 acgtgcttaa atctcgtaat aggagataaa ataattgact aataactgaa tactttttac
    13561 aagctactta aaaattactt aatactgctc tttctcattc ggaaaatcat tgctttttac
    13621 atctgaaata tactgtgtag tggcttcttt tatttggtcg tacaattcta aatagcgacg
    13681 taaaaaacgg ggtttaaatt cgtgggtaat gcccaacata tcatgagtaa ccaaaacttg
    13741 tccgtccaca ccgccaccag caccaatgcc aattacagga atagaaatgg tcttagcaac
    13801 tttttctgcc agatgtgccg gaattttttc aagcactaaa gcaaaacaac caatgctttc
    13861 taaatatttg gcatcttcaa taagctgttc agcttcatct tctgcctttg cacgcaccga
    13921 gtaggtaccg aatttgtaga tggattgcgg cgttaagccc aaatgtccca ttaccggaat
    13981 gcctgctgtt aaaattcgga tgatagattc accaatttcc tgcccgcctt ctaactttac
    14041 gccatgtcct cccgtttcct tcattactcg aatggctgca ttcagcgcct ctaatgaatt
    14101 gccctgatac gaaccaaaag gcaaatctac cacgacaaaa caacgcttaa tggcacgcac
    14161 cacactttgt gcatggtaaa tcatttggtc taatgtaatg ggtagggtgg tttcgtgccc
    14221 cgccatcacg ttggatgccg aatcgcctac cagtaaaata tcgataccgg catcatccag
    14281 aatttttgcc atagaaaaat cataagccgt caacatggaa attttctctc cacgttgttt
    14341 catctctttc atcttgtgcg tggtaacgcg ctttatttca tttgtagccg ccgacattat
    14401 cctaaatttt aggtaataaa ggtagcattt atttggaatt cttaacctat ccttttatgc
    14461 ttgggatatt agccctatct ttgttgtagc aaatcagcga atacaaaaac taattttatg
    14521 aagttcttaa ataccatgac agatgctgaa ttggtagagt ttggcaaaaa aaaccacgac
    14581 gcctatatca atgctcagcc atttccaagc ggttattacg acaatctttt taatccggaa
    14641 ttattgcgcg aaatcataaa agaatttcct gtaattggca aagaaaaaga agacatacaa
    14701 tatagcaatc ccaacgaggt aaaactggca acaaaaggcg aatatcgttt tggtccaaat
    14761 acaaaagcat ttgtgcatta cctcaattca cagccctttc tggaatttct tcaaaaccta
    14821 accggtatta aagaaacact tataccggat ccgtattttg aaggcggagg tttccatgaa
    14881 ataaaaccga acggctattt gaaaatgcat gtcgattttc ataaacataa actcacaaat
    14941 ttagacagac ggttgaatat tctggtttat ttgaatgaag actggaaaga tgaatatgga
    15001 ggtcattttg aattgtggga acgcgatatg agtaaatgtg tgacgaaagt tgctccgctt
    15061 ttcaatcgcc tggccatgtt ctctacaaca gatttttcct ggcatggctt accagatcca
    15121 ttaacttgcc ctcctgacag aagcagacgc tctatcgcct tatattttta taccaacggc
    15181 agaccggaag gagaagtaaa taccggggat aaagcccgta ttactaccac atttgctacc
    15241 cgcgacggac aagattcggg gaaaatgaaa gcttttaacg gatttgttaa cctagccaat
    15301 gatttactac caccgattat tgtgaaaaca ataaaaaaat tcaggaatac atagtttctg
    15361 acgcagtaca aatgggaaat attacctttg cgactgctta attaatgata tggaaa
//
LOCUS       DAOQII010000027        15410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1823_length_15410_cov_5.847932, whole genome shotgun sequence.
ACCESSION   DAOQII010000027 DAOQII010000000
VERSION     DAOQII010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_15410_cov_5.847932"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..532
                     /gene="bcp"
                     /locus_tag="PLF48_02460"
     CDS             104..532
                     /gene="bcp"
                     /locus_tag="PLF48_02460"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNF48214.1"
                     /translation="MLTTGTKAPKISGYDFKGKTTVLYFYPKDDTPGCTAEACSFRDN
                     YASLTKKGFTIVGVSPDNEAKHEKFKTKHELPFQLIPDTDHSIAEAYGVWGLKKFMGR
                     EYMGILRTTFVINEKGIITHVIEKVDTKDASRQLLAVTNS"
     gene            536..1057
                     /locus_tag="PLF48_02465"
     CDS             536..1057
                     /locus_tag="PLF48_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48215.1"
                     /translation="MSRVLSIIVFFYIGIVQLHASGESLNLKLKRIKEQYKSINAYQK
                     YTVFTPDDAASFIGYTDNGASLTGYLRNDTIFKIVVWIGLSNKAIQQEFYYTDEKLLF
                     VFEKISFYEYDKKNNNLDYTKLNLKFSNRYYFDKGKLIYKLCEEEGVVAKTGELLPNA
                     IKYYSLLQDLLQE"
     gene            1119..2201
                     /locus_tag="PLF48_02470"
     CDS             1119..2201
                     /locus_tag="PLF48_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48216.1"
                     /translation="MSNDFESPNQQINKELKCHDCGALLLYAPGTQKLACKYCGAQND
                     IPTETKSYAQVREINFHDYITHQISKEEKQTVSTVSCSNCGATTTLKPNVTSDSCAFC
                     ASPLVISGGTVSTIIKPDYILPFKVDNNNAKSSFSEWLGGLWFAPNDLKKYATLQDKI
                     KGIYMPYWTYDAQSTCEYVGERGEYYYVGDGNNRKRETRWYDVQGVVTNTFDDVFVIA
                     SHSLPDSTTRDLEPWDFKNYTDYNDAYLSGFQAECYQTDVEKGFETAKDVMKNQLRAL
                     AMQQIGGDDQRISNMHIQHNDVTFKHVLLPIWISAYQYNNKVYRFIINGRSGKVVGER
                     PYSWIKIALAVLAALVLIGFIASLRS"
     gene            2245..2664
                     /locus_tag="PLF48_02475"
     CDS             2245..2664
                     /locus_tag="PLF48_02475"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNF48217.1"
                     /translation="MKFKEIIAVTGIPGLKRIVAQRGDGLILSEMNGEGKKFYSNRQH
                     MFSPLENISMYTDDNDTVALYEVLLAMKKQKETHPPVEPNANNDVLRNYLGIVLPNFD
                     RDRVNVSDIKKLIKWFHILEEWNALEEDQTEAEKTEE"
     gene            complement(2713..5964)
                     /locus_tag="PLF48_02480"
     CDS             complement(2713..5964)
                     /locus_tag="PLF48_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNF48218.1"
                     /translation="MSEAPIKQMQELLQKSLIKIKTLEAELETVKNSKTISPNEDIAI
                     VGYAFRFPNQINTLQKFWELLIQNKETVTKIPATRFDVDEIFSEDPYAAGKTNTKYGS
                     FLTEDVSFFDASFFGIPPREAKSIDPIQRILLEIVYEAFENAGIPSNEMKGKNVGVYV
                     AVGVSDYIQARLRSGQLEDIDVYDTTGIPFATICGRISYTYDFNGESISVDTACSSAM
                     VALHHASSALQQQEIDMAVVASANLLLTPELFVGLTKLGSNSPSGKTMAFADDADGYV
                     RAEGAAVLLLKRKLDALKNNDTISLLVKGSFVKHNGTSNGFTAPNPQVQSSTIIKALE
                     NAKLNVDDIDFVESHGIGNKITDAMEIQALQQTFRDKNSPLYVGSVKSNIGHMEACTG
                     MPMIFKLMAAMQHQTIPAQINIATLNKDVNWESSIVQISRENMEWKKEANKLRRASIN
                     LSGYSGTNVHTVFEEAPAKPISESMDAPFVFNFSAKEIPALHALVQKYNEDEYWKNYS
                     PTEIAYTLSQRNLFEQRLSVQADSLEQLGQKLQTYANQENDELIFSTPELNNELSLCF
                     QFTGQGAQYTGMCKGLYDKFDVYKNIVDYCDKELEKHIAVSLKDILWIDGSKTNLIHQ
                     TQYTQPCLFVVEFAMAKLLLSFGIQPSVLIGHSIGEITALAIAEVLSLESALKLVAVR
                     GQLMQSISSQNGAMAAVFASGEVIAQYNQDKKVEIAGYNTPQNTTITGDKNDVDLFVK
                     VLRENHIKAVPLQVSHAFHSKAMDEILDAYREFCSTLHFAQPQIPIASNIDGKIIKEN
                     EFNADYLCLQLRQAVHYLQGIENIQLQYQNIAFIECGPNPVLNNLAKHIVDGKQHYFL
                     SAAKQKGDDVAQFYAVLQQLFVLGSSVNWQNLYEGKKINRVLLPNYAWQWKSYWYSPI
                     RHKKTSDDVKSNEHKPDKNLKGTSSSFQKNGDKRLQKEMLFAVMQMEAVKILGLEAGQ
                     KPNPQKPYREQGFDSMMSGEFLMRMEKQVNAELSMEILHQYNTPELLHRYLIDTYYGG
                     GIVETQNAVTLSDLMFDTASVDFTVEDWHKNNPDDGFFLRIFKKIDSKLPKVK"
     gene            complement(5968..10509)
                     /locus_tag="PLF48_02485"
     CDS             complement(5968..10509)
                     /locus_tag="PLF48_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF48219.1"
                     /translation="MPESEKYAEQIKQALSAIKSLKAQLVAEQKKLHEPIAIVGMAMR
                     LPGGIENEQQLLKVLLDNTDTIEDIPENRFDAKALYDEHGGEGKIILKQGGYLKNIDQ
                     FDASFFDITRVEAESLDPQQRLLLEVTYEALENAGIPSEDLKDSNTSVFTGITNVDYQ
                     KKHFRSGDYKLVNPYSYTGSAVCANSGRISYFFGLQGESVAVDTACSSSLVATHLAVQ
                     SLRNRKSDLAIVGAANLILSPELTITFTALNGLSPTSRCQPFSDNANGFVRSEGCAVI
                     ILKRLEDAKRDNNPILAVIKGTAVNQDGKSNGFTAPSVAAQTKLIQAALDDAQLYIND
                     INYIEAHGTGTKIGDPIEIEAITNVIESARNPLTNKLLVGSVKSNIGHTESVAGLAGI
                     LKTIVSMQHDILPKSLHSETLNMLVDWNQNKLTVVQENSPWENKIKTVGISSFGVTGT
                     NAHIILQNYTADEEITNLYNTNDFYLLPLSTKNDESLVLLVKSYISFIENSKAELADI
                     CATAALSRNQLKCRKLFVAENKEELIQQMKDFVETPTNERTIFEDDDFKKNVFVFPGQ
                     GAQWHGMGLQLIKHEPEFKQALIAYNECLKKYVEWDVFDVLNNKDNALFNQLEVIQPI
                     LVGISIALSELWKSKGVLPDAVLGHSLGEVAAAYCAGCITMDEAAKIITIRSRLMKEK
                     SGQGLMLATDLSTADASAIENEFAGKLTIAVVNSKNSIVLSGDSDVIKTVATRLENEN
                     RFAKEINVDVASHSHHMSGIDDQLKQELTTLQPKNSTIQFYSTALNRSVKGEELDENY
                     WKNNLRNTVRFDGGVQYFLKQGDVLFIEMSPHPTLLHAINENIDETKTKAIAVGSLIR
                     DKDECLAFYNHIGKVFESNSVFNWHLFFPSKGKYVQLPNYAWQKERFWFDEQPEVISV
                     VKEDKKQPAVQMTKTVSLMETAWKEISLQTSPLAGKKILLLCKDLDVQETFISLLEEQ
                     ENEVFAFDVDVTLHEQIDFIICADALCDNNKFIVDTDILIRLQIALKQIESQKNQASC
                     IALTSGLFNHYAGFNADAALLNGVFKSLTNENTQHNFKVVDIPVLYTEEDLKNCLQLI
                     TAENKFKLISLDNNKTYIQQLIQPDSTSADTALNIDFNKTIMVIGGTSGLGLKTAEWL
                     SEKGATYLVLVSRSGEKEETKPVIHRILQNKTQLAVFKGDMSDKKELLKCKTTIENDM
                     PPIGTVIHAAAVLQDRLFFDSSRDEMETVLQAKVKIAQHLHDVFSSPEVQLIFYSSLA
                     DIVGTMAQAVYSGSNYYLDNLTHYRKQKGLPTKTVNWGNIAEIGLAAKEENRGKQLEE
                     QGLGLINIQDIEAYLQLLFSSDKPQLITAKINFEQWKKNYPMVLQDVYFENFIQENIV
                     QITSEERTAFNQPNLDLAQKWIKSRIKDFVAEITKIPSTKLKDDDTFKSIGIDSLMAL
                     QLKNKIQSAFQLSLNVSSVWSYPTADKFTRFLSDELKLTQHYNTDASSKETSKTKDIE
                     QEIKELSLEELMRQLNEKL"
     gene            complement(10509..>15410)
                     /locus_tag="PLF48_02490"
     CDS             complement(10509..>15410)
                     /locus_tag="PLF48_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF48220.1"
                     /translation="VILYNNYTELKETLKDVKQQTTTVEIIESDTCFKHPKIAFVFPG
                     QGAQWHGMGKALYLAEPVFKDCIEQIRTAILNYADWDLIEVLFADEDRLTDIAIIQPA
                     LVAVEIALAKLWMHYGIIPHAVIGHSMGEVAAAYISGSISIDEAACIICTRSKLMRKK
                     SGMGAMAYIASPHAEVQKFLDSNNYKHVHIGVVNSPKSAVITGDAHEIEQIVAAFEKM
                     EIFSRLIKVDVASHSYQMDELKSELKQQLSHLQPKQPNIDFYSTVLGHKINNTTSLDA
                     TYWADNLREPVQFADTLQSLIADGVQLFIEVSPHPVLTQAIAENIESASADAVVIEST
                     ERDKDDVEQFTRSLAKAFCNGAFVNWRNYYGSQYEKIRLPEYPWQKEYYWLKEEQNVS
                     GKTRLINGHAAHPFLQRYSYMAEGIKTHIWESVISLISFPFLSGHKIGDTVVFPAAAF
                     LEIGFAAYHELTGFTSCKMDNISIRNPLYLNEDSSAKIKIVLQQKIGDLYAFGIYADD
                     ETEHFVAEILLKEKHSEAEKFTSSQLPHHLIGKDHYAYCTNIQLNYEQEFTPIKEIRY
                     DADLYESNIELHAIDGLSFYFHPAWLDGMLQTALVPAYLQTQTTYIPYTAKRFSITKL
                     PQGPAGHAKVYVMQKAEDEYCFKLEVYDEVGLCAVFENLIFKILEQNDTLIEEEKKYY
                     RISTIKPDLTALGEKNPIRIIHNNLGSESVYEAFVNSGHQTAYLDIENYSVESFKSAI
                     RQAKLTPDTQLIFIPDEADTWEDADSAMEAQQNTILPLTCLVQALAQTETLVRLWCIT
                     QHAQSIDSDEELSATHAMIPAFMRVLWNEHFELKPSTIDIDGVADIAELPEIIQQYCQ
                     ENELIIRNKTIHAVRLEHDLIIHHQPEIVAGDIPFETSMREVGVIDNLFYKIKYLPVL
                     QEDEIHIEVEAAGINFMNLMSVFGIYPGKVNGFGTLGIECVGTIKAKGSAVHHFDIGD
                     RVFGMAYHTLTSDLITNAASFTKVPDGFSVDDLATIPVVFLTVYFSLVEQARIRKGEK
                     VLIHAATGGVGLAAIQICRLYDCEIYATAGSETKRQYLRDMGILHVYDSRTLDFYDEI
                     LRDTEGKGVDVVLNSLSGEALYKSLDLLDDFGRFVEIGKKDVYENTHIGLKVFKKSLS
                     YFMVDLEKMLFERPEILGELMIEISSLLNEKSIMPLPYTAFGIAEVKDAFHMMQTSSH
                     IGKIIIRFNNKEHISIEDNGMFRCLHQATYLITGGYGGLGMAFANMLAENGAGNIILI
                     GRSAPKEHVLLQIEELRMKGVNVITAFADVSDYDSLKSIIETIDSTTPLKGVFHMAGL
                     LDDSAIANLTSEQFYKVLSPKIAGAINLHELTKNLDLDVFVLFSSSTVLFGSPGQAAY
                     VSANAFMDALAYKRQQLNLPAQSIQWSTISDAGMAAFDDNKTKRLHDEGILPLTAKEA
                     TSTYQFIAYDEHPNIGAFQIDVLKWQQTYLSAKANPYFSQLTKNAVIQHQTNRAVPFR
                     EELMMLNDNQLAENKVEEKLKDIISKTTKIHPDKITSSSTFKSLGVDSLMSIQLKNQL
                     EKTFELSLSVTAFWNHSSIKAYTRFLMDKLQVQSTVQAEQINKISTNEIKPVADASKE
                     TTETATDELDELSKLLDDELNAL"
BASE COUNT         4486 a         3025 c         2838 g         5061 t
ORIGIN      
        1 atttactgcc accgataatt tggctttaaa tgaattgaaa attattgtta ccgaccaagt
       61 gaataatcag actatcttta ctcaaacatt taaaaactaa aatatgctta ctacaggaac
      121 caaagctcca aaaatttcgg gctatgattt taaagggaaa acaacagtcc tctattttta
      181 cccaaaagat gatacgccgg gctgtacggc agaggcttgc agtttcagag ataactatgc
      241 ttcattgact aagaaaggat ttaccattgt gggggtaagt ccggataatg aagctaaaca
      301 tgaaaaattt aaaacaaagc atgagttgcc atttcaattg attccggata ctgaccacag
      361 tatagctgaa gcatatggtg tttgggggtt gaagaaattt atgggcagag aatatatggg
      421 cattttgcgc actacctttg tcattaatga aaaaggcatc attacgcatg tcattgaaaa
      481 agtagatact aaggacgctt ccaggcaact gcttgctgtt acaaattcct agtctatgag
      541 cagagtattg agtataattg tattttttta cattggcatt gttcagttgc atgcttctgg
      601 agagtcgtta aatcttaaac taaaacgaat taaagaacaa tataagtcaa taaatgcgta
      661 tcaaaaatac accgtattta cacctgatga tgcagcaagt tttattggct atacagataa
      721 tggtgcatca ctgaccggat acttgcgtaa tgatactatc ttcaagattg tagtctggat
      781 aggcttgtct aataaagcca ttcagcaaga attttattat acagatgaga agctgttatt
      841 tgtctttgaa aaaatcagtt tctatgagta tgataaaaag aacaataatt tggactatac
      901 aaaattaaat ctgaaattct cgaaccgata ctattttgac aaaggcaaat tgatttataa
      961 attgtgtgaa gaagaaggag ttgtagcaaa aactggagag ttattgccga atgccattaa
     1021 atattattca cttttgcagg atttactgca agaatgattt ttgtttgtaa catctaattt
     1081 aaaggcataa atttactttt aacaaaaaac caaaaactat gtccaacgat tttgaatcac
     1141 cgaatcagca gattaataaa gaactgaaat gccatgattg cggggcattg ctcttatacg
     1201 caccgggcac acaaaagtta gcctgtaaat actgtggcgc acaaaacgat attccaaccg
     1261 aaacaaagag ctatgcacaa gtacgggaga ttaattttca cgattatatc acccatcaaa
     1321 tttcaaaaga agaaaaacaa accgttagca cagtatcttg ttccaactgt ggtgctacta
     1381 ccacactaaa acctaacgta accagcgata gctgcgcatt ttgtgcttcg ccattggtta
     1441 tctccggagg cacggtttct accatcatca aaccagatta tattttgccg tttaaagtcg
     1501 ataataacaa tgcaaaaagc tctttcagtg aatggctcgg cggtttgtgg tttgctccaa
     1561 acgatttgaa aaaatacgct acccttcaag ataaaataaa agggatttat atgccttact
     1621 ggacctatga tgcacaatct acctgcgaat atgttggaga aagaggggag tattattatg
     1681 taggcgatgg caacaacaga aaaagggaaa cgcgctggta tgatgtacaa ggggtggtta
     1741 ccaatacttt tgatgatgtt tttgtgatag ccagccattc tttgccggat agcactactc
     1801 gcgacttaga accctgggat tttaaaaatt ataccgatta taatgatgct tatttgagcg
     1861 gatttcaggc ggagtgctat cagacagatg tagaaaaagg ctttgaaacg gcaaaggacg
     1921 tgatgaaaaa tcaacttcgt gcgttagcta tgcaacaaat cggcggcgac gatcagcgca
     1981 tttccaatat gcatatccaa cacaatgatg ttacctttaa gcatgtatta ttacccattt
     2041 ggataagtgc ttatcagtat aacaacaagg tatatcgctt tatcatcaac ggacgcagcg
     2101 gaaaggtggt aggcgaacga ccatacagct ggataaagat tgcacttgca gtattggcag
     2161 cgttggtgct tattggtttt attgcttcat taagaagttg ataaatccat acatttgctt
     2221 acttttgtgc gttaaaaaca acaaatgaaa tttaaagaga ttatagctgt aaccggtatt
     2281 cccggattga aacgtatagt agcacaacgt ggcgatggtt tgattttgtc tgagatgaat
     2341 ggagaaggaa agaaatttta ttccaacaga cagcacatgt tttcgccttt agagaatatt
     2401 tccatgtaca ctgatgataa tgacactgta gctttgtatg aggtgttgtt agccatgaaa
     2461 aaacaaaaag aaacccatcc gccagtggaa ccaaatgcaa ataatgatgt acttagaaat
     2521 tacttaggca tcgttttgcc caatttcgac agagacaggg tgaatgtttc cgatattaag
     2581 aagctgataa aatggtttca tattttagaa gaatggaatg ccttggaaga agaccagacc
     2641 gaagcagaaa agacagaaga ataaaagaat aggtgaaaaa taagacaaca aattttatta
     2701 tataatattt cgctacttca cttttggcag tttgctgtca atctttttga agatgcgcag
     2761 aaaaaaacca tcgtctgggt tgtttttatg ccagtcttct acagtaaagt ctacactagc
     2821 agtatcaaac attaaatcgg ataaagtaac agcattttgt gtttctacaa ttccacctcc
     2881 atagtaagta tcaatgaggt accggtgcag caattccggt gtgttgtact gatgcaggat
     2941 ttccatgctt aattcggcat tcacttgttt ttccatgcgc attaaaaact ctccactcat
     3001 catgctgtca aacccctgct cgcgataggg tttttgcggg ttcggttttt gtccggcttc
     3061 caagcctaaa atttttacgg cttccatttg cataacggca aacagcattt ctttttgtag
     3121 acgtttatcg ccattttttt gaaaagagga agacgttcct ttcaagtttt tatccggttt
     3181 atgttcattt gatttgacat catcgctcgt ctttttatgc ctgattgggc tataccagta
     3241 agacttccat tgccaagcgt agttgggcaa taacacccga tttattttct ttccttcata
     3301 aagattttgc cagtttaccg aactgcccaa aacaaacaat tgttgtaaaa cagcataaaa
     3361 ctgtgcaacg tcgtcacctt tttgcttagc cgcagataag aaataatgtt gttttccatc
     3421 tacaatatgt ttcgccagat tgtttaaaac cggattagga ccacattcta taaaggctat
     3481 attctgatat tgcagctgaa tattttcaat gccttgtaag taatgaacag cctgacgcaa
     3541 ttgtagacat aaataatcgg cattaaattc attctcttta attattttcc catctatatt
     3601 tgaggctatc ggtatttgcg gctgggcaaa atgaagtgtt gaacaaaact cacgataggc
     3661 atccagtatc tcatccattg ctttagagtg aaaggcatgc gacacctgca agggtactgc
     3721 tttaatatga ttctcccgca acacttttac aaaaagatcc acatcatttt tatctccggt
     3781 aattgtggtg ttttgcggtg tattatagcc tgctatttcc acttttttat cctgattata
     3841 ttgtgctatt acttcaccgg aagcaaacac ggcagccatt gccccgtttt gtgaagaaat
     3901 agactgcatt aattgtcccc gcactgctac cagttttaag gcactctcca gagataatac
     3961 ttctgcaata gccagtgccg taatttcacc aatgctatgt ccaatcaaca cactcggctg
     4021 tataccaaac gaaagcaaca gttttgccat cgcaaattcc accacaaata aacaaggttg
     4081 tgtgtattgt gtttgatgaa tgagattcgt ttttgagcca tcaatccata aaatatcctt
     4141 taaggaaaca gcaatatgtt tttccagttc cttatcgcag taatctacta tatttttata
     4201 aacatcaaat ttgtcataca agcctttgca catgccggtg tactgtgctc cttgaccggt
     4261 aaactgaaaa caaagagata attcgttgtt taattcaggc gtagagaaaa tcaattcgtc
     4321 attttcctga tttgcataag tttgcagttt ttgccctaat tgttctaaag aatctgcctg
     4381 tacagacagc cgttgttcaa ataaatttct ttgggataaa gtataagcaa tctctgtagg
     4441 agaataattt ttccaatatt catcttcatt atatttctgc actaaagcat gcagagccgg
     4501 tatttctttt gcactaaaat taaatacaaa aggcgcatcc atactttcag aaataggttt
     4561 agccggggct tcttcaaata ctgtatgcac gttagtaccg ctgtagccgg ataaatttat
     4621 ggaagctctg cgcagtttat tggcttcttt cttccattcc atattttccc gcgaaatttg
     4681 tactatgctg ctttcccagt tgacgtcttt atttaatgtg gcaatattta tttgagccgg
     4741 aatcgtctgg tgctgcatgg ctgccatcag cttgaagatc ataggcattc ctgtgcaggc
     4801 ttccatgtgt ccgatattac tctttacaga acccacgtaa agcggtgagt ttttatcccg
     4861 aaatgtttgt tgcaaagcct gtatttccat ggcatctgta attttatttc ctataccgtg
     4921 agattctaca aaatcaatgt catcgacatt gagttttgca ttttcgaggg cttttataat
     4981 ggtcgatgat tgtacttgcg gattgggtgc agtaaagccg ttgctagttc cattatgttt
     5041 gacaaatgag cccttaacta aaagggagat ggtgtcatta tttttcagcg catccaattt
     5101 ccgctttaac aataacactg ctgccccttc agcccgcaca tatccatccg catcgtctgc
     5161 aaaggccatt gttttgccac taggagagtt tgagcccagc ttggtcagtc cgacaaacaa
     5221 ttccggtgta agcaataaat ttgccgaagc cactactgcc atgtcaattt cttgttgctg
     5281 caacgctgat gatgcatggt gtagcgcaac catcgctgat gagcaggcag tatctacaga
     5341 gatactctca ccattaaaat cataggtgta agaaattctg ccacaaatgg tagcaaatgg
     5401 gatgcccgtg gtatcataca catcaatgtc ttccagttgc ccacttcgca gtcgtgcttg
     5461 aatataatcg ctgacaccaa cggcaacata aacgcctacg ttttttcctt tcatctcatt
     5521 tgaaggaatc cctgcattct caaaggcttc ataaacaatc tccagcaaaa tgcgctgtat
     5581 gggatcgata gatttggctt cacggggcgg aataccaaag aatgaagcat caaaaaacga
     5641 aacatcttca gtaaggaagc tgccgtattt tgtatttgtt tttccggcag cataaggatc
     5701 ttcactgaaa atttcatcca catcaaatcg tgttgccggg atctttgtga ctgtctcttt
     5761 attttgaatt agaagttccc aaaatttttg aagcgtattt atttgattcg gaaatcggaa
     5821 agcatagcca actatagcaa tgtcttcatt tggagaaatg gtcttgctgt tttttactgt
     5881 ttccagttcg gcctcaagtg tttttatctt aatgagcgat ttttggagca gttcctgcat
     5941 ttgttttatc ggtgcttcac tcatggctta taatttttca ttcaactgtc gcattaattc
     6001 ttccaggctt aattctttta tttcttgttc aatatcttta gtcttgctcg tttcttttga
     6061 tgatgcatcc gtgttgtaat gttgtgtcag ctttaattcg tctgataaaa aacgggtgaa
     6121 cttatctgcc gtaggataac tccatacgga agaaacattg agtgaaagct gaaatgcaga
     6181 ttgtatcttg ttttttaatt gcaatgccat caaagaatca attcctattg acttgaaggt
     6241 gtcatcatct tttaattttg tgcttggtat tttagtgatt tcagctacaa aatcttttat
     6301 tctggattta atccattttt gtgccaaatc aagattgggt tggttaaacg cagtcctttc
     6361 ttccgatgta atttgaacaa tattttcttg aatgaagttt tcaaaataaa catcttgtaa
     6421 aaccatcgga tagtttttct tccattgttc aaaattgatt ttggcagtaa tcagttgcgg
     6481 tttgtcggat gaaaaaagta gctgcaagta agcctcaata tcctgtatat tgattaatcc
     6541 taaaccttgt tcttccaatt gttttcctct gttttcctct tttgcagcca atccaatttc
     6601 tgcaatattt ccccagttta ctgtttttgt gggtaagcct ttttgcttgc gatagtgagt
     6661 taaattatct agataataat tactgccact atatacagct tgtgccatag ttcctacaat
     6721 atcggctaat gaagagtaga atatcagctg cacttcgggt gaagaaaata catcatgcag
     6781 gtgctgtgca atttttactt ttgcttgcaa tacggtttcc atttcatctc tcgaactgtc
     6841 aaaaaacaat ctgtcttgca atactgctgc agcatgaata acagtgccga tgggaggcat
     6901 atcattttca atggtggtct tgcattttaa taattccttt ttatccgaca tatcgccttt
     6961 gaaaacggct aactgcgttt tgttttgtaa aatgcgatga ataacgggtt tcgtttcttc
     7021 tttttctccg ctgcgactga ccaatacaag atatgtcgct cctttttcac tcagccattc
     7081 tgctgttttt aaacccaaac cactcgtacc gccaatcacc atgattgttt tattgaaatc
     7141 aatatttaat gcagtatctg cagatgttga atcgggctgt ataagctgtt ggatgtaagt
     7201 tttgttatta tctaatgata ttaatttgaa tttattttca gcagtaatta attgcaaaca
     7261 gtttttcaga tcttcttccg tatataaaac tgggatatca acgaccttaa aattatgttg
     7321 tgtattttca ttggttaagc ttttaaaaac accatttaat aaagccgcat ccgcattaaa
     7381 tcctgcataa tgattgaaca atccggaagt aagtgctatg catgaagctt gatttttttg
     7441 actttcaatt tgcttgagtg ctatttgtaa tcttattaaa atatcggtat caacaataaa
     7501 cttattatta tcacagagtg catcggcaca gataataaaa tcaatttgct catgcagagt
     7561 tacatccaca tcaaaagcga acacttcatt ttcttgttct tcgagcaaag aaatgaaagt
     7621 ttcctgtaca tccaaatctt tacataaaag cagtattttc tttccggcta atggcgaagt
     7681 ttgcaagctg atttctttcc atgccgtttc cataagggaa actgtttttg tcatttgaac
     7741 agccggttgt tttttatctt cttttacaac agaaattact tcaggttgtt catcaaacca
     7801 gaatctttct ttttgccagg catagttggg cagttgaaca tatttgcctt tggatgggaa
     7861 aaataaatgc caattgaaga cactgtttga ctcaaaaacc tttccgatat gattgtaaaa
     7921 tgccaaacat tcatctttat ctctgataag cgaaccaacg gcaatagctt tcgttttcgt
     7981 ttcatcaata ttttcattga tggcgtgtaa caaggtagga tgcggactca tctctataaa
     8041 caatacatcg ccttgtttta agaaatactg cacacctccg tcaaaacgga cggtattgcg
     8101 cagattattt ttccagtaat tttcatccag ttcttcccct tttacactgc gattaagcgc
     8161 cgttgaataa aactgtatgg ttgaattttt aggttgcagt gttgttaatt cttgctttaa
     8221 ttggtcatct atgccactca tgtggtggct atgtgaagcc acatctacat taatttcttt
     8281 tgcaaaacga ttttcatttt caagtcgcgt agctacagtt ttaatcacat ccgaatctcc
     8341 cgagagtaca atggaatttt tgctattaac aaccgcgata gttaatttgc ctgcaaactc
     8401 attttcaata gcacttgcat ccgctgtcga taagtcagtt gccagcatta agccctgacc
     8461 acttttttct ttcattaatc tgctacgtat agtaataatt tttgccgctt catccattgt
     8521 aatacagcct gcacaatatg cagccgctac ttctccaagg ctatgtccca atacggcatc
     8581 cggcagcact cctttgcttt tccagagctc acttaaagcg atactgattc caaccagtat
     8641 gggttgtatt acctctaatt gattaaaaag ggcattgtct ttattgttga gcacatcaaa
     8701 tacatcccat tccacatatt ttttcaaaca ttcattatag gcaatcagcg cttgtttaaa
     8761 ttctggttca tgtttgataa gttgcaaacc catcccatgc cattgtgctc cctgtcccgg
     8821 aaaaacaaag acattttttt tgaaatcatc atcttcaaat atcgttcttt catttgttgg
     8881 cgtttccaca aaatctttca tttgttgaat gagttcttct ttgttttcag caacaaacag
     8941 cttgcggcac ttgagctgat ttctgcttag ggcagctgta gcgcaaatat cagctagttc
     9001 tgcttttgaa ttttctataa atgagatata gctttttact aataacacca gtgattcatc
     9061 gttttttgtc gataaaggaa gcagataaaa gtcgttagtg ttgtaaaggt ttgttatttc
     9121 ttcatctgca gtgtaatttt gcaagatgat gtgcgcattt gttccggtaa cgccaaaact
     9181 actaatacct actgttttga ttttattttc ccatgggcta ttttcctgta ctactgttag
     9241 tttattctga ttccagtcta ccagcatatt taaagtttcg ctatgaagac ttttgggcag
     9301 gatatcatgc tgcatagaca cgatagtttt taaaattcct gccaagcctg caacactttc
     9361 ggtatgcccg atatttgatt ttacagaacc taccaagagc ttattggtaa gtggatttct
     9421 tgcactttca attacattag ttatagcttc tatttctata gggtcgccaa ttttagtacc
     9481 ggtgccatga gcttcaatat agttgatatc attaatgtat aattgagcat cgtctaaagc
     9541 agcttgtatt aacttagttt gtgctgctac actgggagca gtaaagccat tacttttccc
     9601 atcctgattg acagcagtac cctttataac agcgaggatg gggttgttgt ctcgtttggc
     9661 atcttccagt ctttttaaaa taataaccgc acatccttcg gagcggacaa aaccattggc
     9721 attgtcagaa aacggctgac agcgagatgt aggtgacagt ccattcagcg cagtaaatgt
     9781 tattgtaagt tcaggagata aaattaaatt ggcagcacct acaattgcca aatcactttt
     9841 tcggttgcgc aaagattgca ctgccagatg agtggcgacc agtgatgaag aacaggccgt
     9901 atctacagca acggactctc cctgtaagcc gaaaaagtaa gaaatccttc ctgagttggc
     9961 acatacggca gagcccgtat aggagtatgg atttactaat ttataatctc cgctgcgaaa
    10021 atgttttttc tgatagtcaa cattggtaat ccctgtaaag acactcgtat tactatcctt
    10081 aagatcttca gagggaattc ctgcgttttc caaagcttca taagtaacct ctaataataa
    10141 tcgttgttga ggatccaggc tctcggcttc tacacgtgtg atatcaaaaa aagaagcatc
    10201 aaattggtcg atgtttttaa gatagccacc ttgcttcaaa ataatttttc cttcaccacc
    10261 gtgctcatca taaagtgctt tagcatcaaa acgattttcc ggaatatctt ctatagtatc
    10321 tgtattatcc aacaatactt tcagtaactg ttgttcgttt tctatcccac cgggcagacg
    10381 catagccatg cccacaatgg caataggttc atgtagtttt ttttgctctg ctaccaattg
    10441 agctttcaat gatttaatag ctgaaagcgc ttgctttatt tgttcggcat atttttctga
    10501 ttctggcatt acaaggcatt taattcgtca tccaataatt tactcagctc gtctaattca
    10561 tccgtagctg tctctgtcgt ttctttactt gcatctgcta ctggttttat ctcgtttgtg
    10621 cttatcttat taatctgttc agcttgtacc gtagactgta cttgtaattt atccattaaa
    10681 aaacgggtat atgcttttat tgaagagtga ttccaaaagg cagtgacaga taatgacagt
    10741 tcaaaagttt tctccagttg atttttcaat tgaatagaca tcagcgagtc cacccctaaa
    10801 gatttgaaag tggatgaaga tgttatttta tcaggatgaa ttttagttgt ttttgaaata
    10861 atatctttca gtttctcttc cactttattt tctgctaatt gattgtcatt tagcatcatc
    10921 agctcttcac ggaaaggaac agccctgttt gtctgatgct gaataacagc gttctttgtc
    10981 aattggctga aatatggatt agcctttgca gaaagataag tttgctgcca tttaagcaca
    11041 tcaatttgaa atgccccaat attaggatgt tcgtcataag caataaattg gtatgttgat
    11101 gtggcttctt tagcagtcaa aggtagtatt ccctcgtcgt gcaaacgttt ggttttgttg
    11161 tcgtcaaatg cagccatacc tgcatcagat atggtactcc attgtatgct ctgagccggc
    11221 aagttgagtt gctggcgttt gtatgccaaa gcatccataa atgcattggc tgatacgtag
    11281 gcagcctgtc ccggcgagcc gaataaaact gttgatgagc taaacaagac aaaaacgtcc
    11341 agatctaagt ttttagtaag ttcatgtaaa tttatagctc cagcaatttt tggcgacagt
    11401 actttgtaaa attgttcact tgtaaggttt gcaatagcac tgtcatcaag caagcctgcc
    11461 atatgaaaca cgcccttaag tggcgtagtg ctatcaattg tctcaatgat tgattttaat
    11521 gaatcataat cactcacatc agcaaatgca gtaatgacat taacgccctt cattctaagc
    11581 tcttcaattt gtaagagtac atgttcctta ggtgcacttc tgccaatcag tataatgttt
    11641 ccggcaccat tttccgccag catatttgca aacgccatac ccagtccacc atagccgccg
    11701 gtaatcaggt aagtagcttg gtgcaaacat ctgaacatgc cgttgtcttc aatgctgata
    11761 tgttctttat tattaaatcg gataattatc tttccgatat gagatgatgt ctgcatcata
    11821 tgaaaagcat ccttaacttc agcaataccg aatgcagtat atggcaaagg cataattgat
    11881 ttttcattta acaggctgct gatttctatc atcagctcgc ctaaaatttc aggtctttca
    11941 aaaagcatct tttccaaatc aaccataaaa tagcttaatg attttttgaa aacttttaag
    12001 ccgatatgtg tattttcata cacgtctttt ttgccaattt ctacaaatcg accaaaatca
    12061 tcaagcaaat ccaagctttt atacagagct tctccggata aagaatttag tacaacatct
    12121 acaccttttc cctccgtatc acgaaggatt tcatcataaa aatcgagcgt acgcgaatcg
    12181 taaacatgca gaatacccat atctctgaga tattgacgtt tagtttcact tcccgctgta
    12241 gcatagattt cacagtcata cagtctgcat atttgaatag ccgccaggcc cacaccgcca
    12301 gttgcagcat gtatcaatac tttttctcct tttctgatgc gtgcctgctc aaccaaacta
    12361 aaataaacag taagaaacac aacaggaatt gtagctaagt cgtcaacact aaatccatca
    12421 ggtaccttag taaaagaagc agcatttgtg attaaatctg aagtgagggt atgataggcc
    12481 atgccgaata cacgatctcc aatatcgaaa tgatgaacag ctgaaccttt tgctttaatc
    12541 gttccgacac actctatacc taaagtgcca aatccgttta ctttaccagg atagataccg
    12601 aaaacagaca ttaaattcat aaagttgata cccgcagctt caacttcaat atgtatttca
    12661 tcttcctgta aaaccggaag atatttaatt ttgtaaaata aattatcgat tacaccgacc
    12721 tctcgcatgc tggtttcaaa tggaatatct cctgcaacta tttcaggctg atgatggatg
    12781 ataagatcgt gttcgaggcg aacagcatga atcgttttat tgcggataat caattcgttt
    12841 tcttggcaat actgctgaat aatttccgga agttcagcaa tgtcagcaac tccatcaata
    12901 tcaatagttg atggttttaa ttcgaaatgc tcattccata agacacgcat aaaagcaggt
    12961 atcatggcat gtgtagcgga aagttcttca tcgctatcga tagactgtgc gtgttgtgta
    13021 atacaccaca ggcgcaccaa ggtctccgtt tgtgctaatg cctgtacgag acatgttaat
    13081 ggcaatatcg tattttgctg tgcttccatc gccgaatcag catcttccca agtgtcagct
    13141 tcatcgggaa tgaatatcag ctgagtgtca ggtgttaact tggcttgtct gatggccgat
    13201 ttaaaagact caacactata gttttcaata tcaagataag cagtctgatg cccgctgtta
    13261 acgaaagcct cataaacact ttctgaccct aaattattat ggataatccg aataggattt
    13321 ttctcaccaa gggctgtaag atctggttta atcgtagaaa tacgatagta ttttttttct
    13381 tcttcaataa gcgtatcatt ttgttccagt attttgaaaa ttaaattttc aaagactgcg
    13441 cataagccaa cttcatcata gacttcgagt ttgaagcagt attcgtcttc ggctttttgc
    13501 atcacatata cttttgcatg tccagctgga ccttgtggta gttttgttat gctaaatctt
    13561 ttagcagtat atggaatata ggttgtttgc gtctgtaaat atgcaggaac taaggcagtt
    13621 tgcagcatgc catcgagcca ggcaggatga aaataaaatg aaagaccatc aatagcatgc
    13681 aattcaatat tgctttcata caagtcggca tcataacgga tttcttttat gggagtaaat
    13741 tcttgttcgt aattcaactg aatattagtg caataggcat aatggtcttt tccaatgaga
    13801 tgatgaggta attgagatga agtaaatttt tcagcttcac tatgtttttc cttcagtaat
    13861 atttctgcga caaaatgttc agtttcgtca tcagcataaa taccgaatgc atataaatct
    13921 ccaatttttt gctgtagtac aattttaatt ttagcagaag agtcttcgtt taagtaaagg
    13981 ggattgcgaa tgctgatatt atccatttta caggatgtaa atccggttag ttcgtgataa
    14041 gcagcaaaac caatttccag aaacgcagca gccgggaaga ctacagtatc gcctatctta
    14101 tgtccactta aaaatggaaa tgaaataagt gagattacag actcccagat atgtgtcttt
    14161 attccctccg ccatatagct gtatcgttgc agaaacggat gtgcggcatg accatttatc
    14221 aatcgtgttt taccggatac gttttgttct tcttttaacc agtaatattc tttctgccag
    14281 gggtattcgg ggaggcgtat tttttcgtat tgagaaccat aataatttct ccagtttaca
    14341 aatgctccat tgcaaaatgc ttttgctaag cttctcgtaa attgttcgac atcatcttta
    14401 tctctttctg tgctttcaat aacaactgca tcagcagagg cactttcaat gttttctgca
    14461 atggcttgtg tgagtacagg atgcgggctt acttctataa ataattgcac cccatctgca
    14521 attaatgatt gtaaggtatc tgcaaactgt accggttcgc gcagattatc tgcccaataa
    14581 gtggcatcta aacttgtggt gttgtttatt ttgtgaccaa gcactgtaga gtaaaaatca
    14641 atgtttggct gtttaggctg cagatgacta agttgttgtt taagctctga tttaagttca
    14701 tccatctggt agctgtgcga cgccacatct acttttatca gcctactgaa tatttccatt
    14761 ttttcaaatg cggcgacaat ctgttctatt tcatgagcat caccagtaat tacagcagat
    14821 ttcggactat ttactacacc gatatggaca tgcttatagt tatttgagtc taggaatttc
    14881 tgcacttctg catgcggtga tgcaatataa gccatagcac ccatgccact cttcttgcgc
    14941 atcagtttac ttctggtaca aataatacat gcagcttcat ctatactgat actacctgaa
    15001 atatatgctg cggctacctc gcccatacta tgcccgatga cagcatgagg tatgatacca
    15061 taatgcatcc acaattttgc caaagcaatc tcgactgcaa cgagagcagg ttgaataatg
    15121 gcaatatctg tcagtctgtc ttcatcggca aaaaggactt caattaagtc ccagtctgca
    15181 tagtttagta ttgctgtacg aatttgttct atgcaatctt taaataccgg ttcggcaaga
    15241 tatagtgctt ttcccatccc atgccattgg gcaccctggc cagggaaaac aaatgcgatt
    15301 ttcggatgct taaagcaggt gtcactttca attatctcta cagtagtagt ttgttgctta
    15361 acatctttga gcgtttcttt taattctgta taattattat ataatattac
//
LOCUS       DAOQII010000028        15209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1862_length_15209_cov_4.671440, whole genome shotgun sequence.
ACCESSION   DAOQII010000028 DAOQII010000000
VERSION     DAOQII010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15209
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1862_length_15209_cov_4.671440"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1258)
                     /locus_tag="PLF48_02495"
     CDS             complement(62..1258)
                     /locus_tag="PLF48_02495"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF48221.1"
                     /translation="MLQTSDALNRVTESATIKMAQLSRDLAAKGVNVISLSLGEPDFN
                     TPKHIKEAAKAAIDSGKYDSYSPISGYLDLKQAICEKLKRDNNLEYKPENIVVSTGAK
                     QSLANLFIGFLNPGDEVVIPAPFWVSYPEQVKLCGATPVYIPTSVDADFKMTAEQLEN
                     AITPKTKFLVYSSPSNPTGSVYTKEEIESFVAVLERHPHVYVISDEIYEHINYTGKHV
                     SIAEFPGMKDRTIIVNGQAKGYAMTGWRIGYIAAPAYIAKACDKLQGQITSGTNSVAQ
                     HATITALLGDQEPTHQFRLAFEKRRDLVLEMLKEIPNVKCNKPQGAFYVFPDMSAYFG
                     KDFNGEMIKDADDLCLYLLHEGHVAAVSGTGFGAPECIRFSYAASEENLTEAFRRVKA
                     ALEKLA"
     gene            complement(1719..2048)
                     /locus_tag="PLF48_02500"
     CDS             complement(1719..2048)
                     /locus_tag="PLF48_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNF48222.1"
                     /translation="MNKDSISGMINAVKAFHEKNGFDIATKSDSTMFYRQNMLMEELG
                     EISECLTKGKGNIAEEHADLFILLLGNCIVMDIDIEQEFWTKMEKIMQRPSKNVAGFN
                     RVSEWEK"
     gene            complement(2067..2705)
                     /locus_tag="PLF48_02505"
     CDS             complement(2067..2705)
                     /locus_tag="PLF48_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNF48223.1"
                     /translation="MAKQKKQLKHIAIAGNIGSGKTTLTERLAKHYGWDPRFEDVENN
                     PYLNDFYQDMPRWSFNLQIYFLNSRMRQLIEIQEGSEIVIQDRTIYEDAYIFAPNLHE
                     MGLMSTRDFENYFSFFKTICKKINPPDLLVYLKASLGTLVSQIEKRGRDYEDNIRLDY
                     LKQLNQYYDNWIENYKEGPLLIINVDDLNYADKPEDLGKVIEKIDAELFGLF"
     gene            complement(2860..3411)
                     /locus_tag="PLF48_02510"
     CDS             complement(2860..3411)
                     /locus_tag="PLF48_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF48224.1"
                     /translation="MQIHQLNHANEIILRVIQPEDIQEFYAFSKYIFYELNEYTIDTP
                     EEFSHDTDIEKQRIEKFNLPGNLLLGAFYDGQLIGVLDFNSNKRKRINHWGKFGISIH
                     HDFQNRGIGTKLVHFLIEWAKQNGQTKMILLSVHANNERAIALYTKMGFVQCGYFKSC
                     IREKDNSFTDSIDMVLYLDALRA"
     gene            complement(3472..4740)
                     /gene="icd"
                     /locus_tag="PLF48_02515"
     CDS             complement(3472..4740)
                     /gene="icd"
                     /locus_tag="PLF48_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNF48225.1"
                     /translation="MSAEKIKMVDGHLHVPDEPIIPFIEGDGTGPDIWKASVRVMDAA
                     VEKAYGGKRKIHWKEVLAGEKAYKQTGSWLPDETLTSIDEHLIAIKGPLTTPVGGGIR
                     SLNVALRQLLDLYACVRPVRYFEGVPSNSKVPDQVDMIIFRENTEDIYAGIEWLEGTE
                     ECNRVVGFLLNDMNVGKKIRFPETSSIGIKPVSKEGSERLIRAALKYALQHKRKSLTL
                     VHKGNIMKFTEGKFKEWGYQLAEREFGDQVFTWAQYDKILAEDGQVAADEAQKAALAA
                     GKMLVKDAIADNFLQQIILRPSEYDVIATLNLNGDYISDALAALVGGIGIAPGANINY
                     LTGKAIFEATHGTAPKYANQDKVNPSSVILSGVMMFEFMGWLEPAELILKGMEKAIEA
                     KTVTYDFHRLMEGATLLKCSEFGEAIISHF"
     gene            5256..5990
                     /gene="hisA"
                     /locus_tag="PLF48_02520"
     CDS             5256..5990
                     /gene="hisA"
                     /locus_tag="PLF48_02520"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNF48226.1"
                     /translation="MLIIPAIDIIDGKCVRLTKGDFNTQVTYNENPVEQALFFEDAGF
                     SHLHLVDLDGAKQGKTVNWNVLEEIVKATRLTIDFSGGIKTKEQAQKAFDTGANQICV
                     GSLAVKSPKEFIDWIWAFGEEKLILGADVLNNKIAIEGWAATSQKTITEFLDYYFEEG
                     IDYVLCTDVSKDGMMMGPSLELYRTILSAYEEIKLIASGGITTLEDILQLQALGCYGA
                     VVGKALYEAETREAILNYQKNKLHAD"
     gene            5980..6732
                     /gene="hisF"
                     /locus_tag="PLF48_02525"
     CDS             5980..6732
                     /gene="hisF"
                     /locus_tag="PLF48_02525"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNF48227.1"
                     /translation="MLTKRIIPCLDIKDGHTVKGVNFVDLIHAGDPVELAEKYVEQGA
                     DELVFLDITATVENRKTLVELVSRLSEKINIPFTVGGGISTLQDVDALLNAGADKVSV
                     NSAAVKNPDLINELAAHFGTQCIVLAVDTKQDEEWMVYVHGGRTPTSLKTKEWVKQAQ
                     DRGAGEILLTSMNHDGTKNGFALDITLWCSENLHIPVIASGGAGNEKHFEDVFFKAKA
                     DAALAASIFHFGEISIPHLKKYLLDKDICVRV"
     gene            6806..8641
                     /locus_tag="PLF48_02530"
     CDS             6806..8641
                     /locus_tag="PLF48_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNF48228.1"
                     /translation="MASPFSSSKERPKISKDSLKKSLKVYSFIKPYQYSFYLGLLFLV
                     LSSLTVLILPMLLPRLADTAQGIPVQLFKINGKYLIDCKTSWDIVKVTMVFIVLQGVF
                     SFFRIYIFAQTNERVLKDIRSSVYNKTITSPIIFFENNRVGELISRISSDITNVQDAL
                     SLNIAEFLRQVVTLLAGIVYVFVSSKELTFFILCTIPVLVLLALFFSRYIRNNASRTQ
                     QALAQSNIIVDETFHNINVVKAYVNEYFESNRYVKSITAVKDLALRAALFRGAFISFL
                     IVGVMTVLIMVLYKASIYVHNFYLHVEPSMSVGQLLSFIFFTVFIGGSLAGMGDLYGK
                     IISALGSSDRIISILDMPSETKIEQVPLQKINGEIEFNKVNFSYPSRKETGVLHDISM
                     HIRNGEKVALVGSSGAGKSTILQLLLRFYPVNSGDIKVAGKNIYDYDVQELRSNIALV
                     PQEVMLFGGTIRENIAYGKTNATDEEIKQATIKANAYDFILKFPDGFDTIVGERGVKL
                     SGGQRQRIAIARAILKDPAILLLDEATSALDAESEKLVQDALNKLMENRTTIVIAHRL
                     STIRTVDKIFVMNHGVIVEEGTHDELSSTENGLYSNLLKLQYQIE"
     gene            8667..9389
                     /gene="bshB1"
                     /locus_tag="PLF48_02535"
     CDS             8667..9389
                     /gene="bshB1"
                     /locus_tag="PLF48_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNF48229.1"
                     /translation="MALRLDILAFASHPDDVELSCSGTLMKHIDKGYKIGIVDLTEGS
                     LGSRGTVATRYKEAKKASEIMGITVRENLQFRDGFFEINEAHLLKVITVIRKYQPAII
                     LANAIYDRHPDHKRGGDLVSRANFLSGLIKIETEANGKKQQLWRAKKLFRYIQESYLK
                     PDFAIDISAYAQKKLACIQAFSTQFYNPESTEIETPISTKNYLDYVLSKDKVFGKQIN
                     VDYAEGFICEQPSGIEDLFHMQ"
     gene            9397..10785
                     /locus_tag="PLF48_02540"
     CDS             9397..10785
                     /locus_tag="PLF48_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48230.1"
                     /translation="MKSKSLFLFYGIISFLILAIFSFYFFRERIAFTDLSFHLFTLVR
                     TAKFAIFNYRYTAAFSQMLPLFAIKCEQSLYVISAFYSFSFVLVQFCVFLVIVFYFKN
                     YFLAACLLLLQVLQIRHTFYWTGSEFIPGVAFLFLPIAYIEKRTSSVSKIRHAVMLIL
                     FLLPVIFSHPVIVFPFLYIVVFFFLTQKGKRKSLIWLTLLFLLLFIAKYFLLKSNYDA
                     GALSSLNPKKLKWHIMLHYPSHQLFINNLWKHYNLMAVIFCFSLFLLAIKRNYLLAVL
                     LVTVCLLYIALVNLNYPQGAELFYIENLYLPLSVFIILPLVYSLFNYIKNTMLIHAFL
                     LLILLVGLIQIYQTHTPYTARIGWMYGIMDKAKSANQTKIILPVRDVPLDTLGMAWGS
                     PYEFWILSTLKYKSTTSVIIEETPHEYDWIKDSKNIFITKWGNFAYDQLNPKYFVLTD
                     SSSYQIVQAKHE"
     gene            10855..11883
                     /gene="mutY"
                     /locus_tag="PLF48_02545"
     CDS             10855..11883
                     /gene="mutY"
                     /locus_tag="PLF48_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNF48231.1"
                     /translation="MESFSKKLLEWYNPKLRNLPWKNTKDAYKIWLSEIILQQTRVEQ
                     GTPYYLAFIKKYPKINLLANAPLDEVLKLWEGLGYYSRARNLHASAQQVLHEHKGNFP
                     KSYEELKKIKGIGNYTAAAIASFAYGLPHAVVDGNVYRVLSRVYGIFTPIDTNQGKKQ
                     FETIAQKLLDKKNPSVYNQAIMDFGSLVCKPKNPLCKACPFQDECQALAMQKIGELPV
                     KSKKTSMQKREFYFFVYKNKGYTYIVQRDQQDIWKGLYEFPFVEIKSEKDKSRFKKLK
                     PVYTDKQQLSHQIIHADFFEIEEKTKLMQFFPDILQFKKIKITTLENYAFPKIIRNYL
                     QQAKLFLS"
     gene            11941..12396
                     /locus_tag="PLF48_02550"
     CDS             11941..12396
                     /locus_tag="PLF48_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF48232.1"
                     /translation="MSFNKVFLIGRLGKDPDVRKVNATTTVCNFPFATNEVYKNQDGS
                     YTETTEWHNIVMWRGIAERAERILKKGSHVFIEGKLRTRSWDDKDGNKRYTTEIVVEN
                     FQLLEKREASGQTTGIVNFGDAQKSSGDDTHAAQDTNLTEPTFDDDLPF"
     gene            12420..13775
                     /gene="gldE"
                     /locus_tag="PLF48_02555"
     CDS             12420..13775
                     /gene="gldE"
                     /locus_tag="PLF48_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNF48233.1"
                     /translation="MDDGIPLCSFLLNIEFYSWNEIFNPFKIQYAVYGIAILILLSFT
                     AILCALEVAIFMFSNAEVKRLEQSSNKTDALILHYILNPNRFIATFVLTTTLFTLAIV
                     LLFEALLEQWIREDFIHQHSTLLFIIKLCFETFIIVLFAEVIPKLYATQKYYQVCRAV
                     IYPINILDKIFSPFVTLMMKFSDVIEKRLIVAKQTLTAQDIHDALEITESQSTEVSKE
                     RTKILKGIVKFGNLNVSQIMRSRIDMVAIDHDANFHDLLTIVRESGYSRIPVFENDMD
                     RITGIIYIKDLLKYLHEKNNFDWKKLMRPPLFVPENKKIDDLLEDIQERHIHIAIVVD
                     EYGSCCGLVTLEDVLEEVIGDIKDEFDDASEIDFKKLDNNNYIFEAKTALNDVCKVMD
                     LPTETFDEIRGTADSLAGMLLELNNEIPKEGDEIKYGKFTFLILEASITRIIRVKISI
                     A"
     gene            13807..14406
                     /gene="gldD"
                     /locus_tag="PLF48_02560"
     CDS             13807..14406
                     /gene="gldD"
                     /locus_tag="PLF48_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNF48234.1"
                     /translation="MVKKKHFLLFLIMVFILVSCKTNYTPKQPGYFRIDFPAERKYTE
                     YSCDECPFTFEYPVIGAIQKDSTDMQEIHNHPCWFNIIFPEYKAKIYLSYTAINAGNP
                     LDKLIADSYKLTFKHTLKADYIDESQITGKNPHVSGILYDVGGDAASGVQFYVTDSTK
                     NFIRGSLYFFSPPNADSLAPCIQYFREDVLHFVETLKWR"
     gene            complement(14413..14946)
                     /locus_tag="PLF48_02565"
     CDS             complement(14413..14946)
                     /locus_tag="PLF48_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48235.1"
                     /translation="MQLFILIFAVVLILAGIYLFITQAHRSEVWEYTAIAFQLIGIVA
                     LITIWQMHYFIPDASAGKDMLYSWIYQWVDEKLNIYLSAFICLVYVVLQYYGQQLYQQ
                     SKIKKDGIVVNARVRAIEKKEKKADHIKIEYKVQDKTIVKTLIKSKHDFVPEQEVPVI
                     YSASAPTIFMLKSDEMI"
     gene            complement(14956..>15209)
                     /locus_tag="PLF48_02570"
     CDS             complement(14956..>15209)
                     /locus_tag="PLF48_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48236.1"
                     /translation="YRQQILNISCKGETKWDESVVITDSGMEKLNDLEADGILQWQPG
                     ELKLTHMGWGFLRNVCAVFDKRMNDDRQKQDAPKFSQAI"
BASE COUNT         4825 a         2766 c         2735 g         4883 t
ORIGIN      
        1 tgagaaaaca agtcatcgtg cagttgaact aagggtgatg gcaactggaa cataagaaaa
       61 cttatgctaa tttctctaat gctgctttta cgcggcggaa agcttctgtt aggttttctt
      121 cggaagcggc atacgaaaaa cgaatacact ccggtgcacc gaaacctgtt ccgcttacag
      181 ctgccacgtg cccttcgtgc aacaaataca aacacaaatc gtcggcatct tttatcattt
      241 cgccgttaaa atctttaccg aaataggcac tcatatccgg gaaaacataa aacgcacctt
      301 gcggtttatt acattttaca ttcggtattt cttttagcat ttccaacacc aaatccctgc
      361 gtttttcaaa ggcaagacga aattgatgtg taggctcctg atcgcccagt agtgccgtaa
      421 tagtagcatg ttgcgccacc gaatttgttc cggatgtaat ttgtccttgc aacttatcac
      481 aagctttcgc gatgtaggca ggcgctgcaa tatatccaat tctccagccc gtcatcgcat
      541 agcctttcgc ttgtccgttc acgataatgg tgcggtcttt cataccagga aattcagcta
      601 ttgacacatg ttttccggta taattgatat gttcgtaaat ctcatctgaa attacatata
      661 cgtgtggatg cctttcgaga acggcaacaa aactttcaat ttcttctttg gtataaacag
      721 aacccgtggg attgctgggt gaagaataaa ccaaaaattt tgttttaggc gtgatggcat
      781 tttccaattg ctccgctgtc attttgaaat ctgcatctac cgaggtaggt atataaaccg
      841 gagtagctcc acacagtttt acttgctcgg gataagacac ccaaaatggt gcaggaataa
      901 caacttcatc tcccggattt aaaaagccga taaataagtt tgccaaagat tgttttgctc
      961 ctgtagaaac aactatattt tcgggtttat attccagatt attatcgcgc tttaattttt
     1021 cgcaaatcgc ttgtttcaaa tccaagtaac cggaaatagg tgagtaagaa tcgtatttac
     1081 cgctgtcaat ggccgcttta gctgcttctt tgatatgctt gggtgtgtta aaatcgggtt
     1141 cgcctaatga caagctgatt acatttacgc ctttggcggc aagatcgcgg ctaagctgtg
     1201 ccattttgat agtggctgat tccgttacac ggtttaaagc atctgatgtt tgtaacatat
     1261 ttgtaaattt cagcataaaa atactgaatc tcttaaacta aaaacaaatt ggattattat
     1321 gttaagataa aacttgatga atgatgaatg atgaatgatg aatgatgaat gatgaatgat
     1381 gaatgatgaa cgatgaatgt tgaaagatga atgttggatg attattgctg agctactctg
     1441 tttgtttggt actttttttc ttagtcaaaa aaagtaccca aaaaagacaa ggcaaaaata
     1501 tcgctctgca ctaaagcaca cgaactgcta ccgatttttg ccatcccacg cttttttgca
     1561 tcataccttc tataataatt atcgtttgtt tttagtgtag ttatagattt gagacggtga
     1621 attataaatt ttatagttta tatttaaaag aataaatact aaaacatacc aaactttctt
     1681 attttatcac taattagcaa tgactacgct cctttcatct acttttccca ttcactcacc
     1741 cgattaaaac cggcaacatt tttagaggga cgttgcataa ttttttccat ttttgtccaa
     1801 aattcctgtt caatatcaat atccattacg atacaattac ctaataataa aataaataaa
     1861 tcggcatgtt cttccgcgat atttcctttt cctttagtca ggcattcact gatttcgccc
     1921 aattcttcca tcagcatatt ttgtcggtaa aacatggtcg agtcactttt agtggctata
     1981 tcaaacccat tcttttcgtg aaatgcttta acggcattta tcataccgga aatgctgtct
     2041 ttgttcataa taaataagat aaaaaattaa aataaaccga ataactccgc atcaattttt
     2101 tcgatgacct tgcctaaatc ttccggttta tcggcataat tcaaatcatc cacattgatt
     2161 atcaaaagcg gaccttcttt atagttttca atccagttgt cgtagtattg attgagttgt
     2221 ttcaaataat ccagacggat attgtcttca taatctctgc ctcgcttttc aatctgactc
     2281 accaaagtac ccaacgatgc tttaagataa accaataaat cgggaggatt gatttttttg
     2341 caaatcgttt tgaaaaagct gaaatagttt tcaaagtcac gcgtactcat caaacccatt
     2401 tcgtgcaaat ttggagcaaa aatataggca tcttcatata tggttctatc ctgaattaca
     2461 atttcactgc cttcttgtat ttctatcaat tgtcgcatac gactattcaa aaagtaaatc
     2521 tgcaagttga atgaccagcg gggcatatcc tgatagaaat cgttgaggta aggattattt
     2581 tccacatctt caaagcgagg atcccaaccg tagtgttttg ccagcctttc tgtgagtgtt
     2641 gtcttaccgg agccaatgtt tccggctatt gcgatgtgtt ttaattgttt tttttgcttt
     2701 gccacaagag tgtgttttat cgttttctaa atctgcacaa ctaagctaaa aatttatttg
     2761 acagcagaca aaataaatgc aaataaaaaa acggaaagtg gataaattgt tttgaaaacg
     2821 tgaacgggcg aatttggcaa acagagaatc tatttttttt tatgctctta gcgcatcaag
     2881 ataaagaacc atatctatgg aatccgtgaa ggaattatcc ttttctctga tacaactttt
     2941 gaaataaccg cattgaacaa atcccatttt agtgtacagg gctatagctc gctcattgtt
     3001 agcatgtacc gagagcaata tcatttttgt ttgcccattt tgttttgccc attcaatcag
     3061 aaaatgcacc aattttgtcc cgatgcccct attttgaaag tcgtgatgaa tactgatacc
     3121 aaatttcccc caatggttta tacgctttct tttgttgcta ttaaaatcca agacacctat
     3181 aagttgtcca tcataaaaag cgcccagtag caaattgccc ggtagattga atttttctat
     3241 tctttgtttt tcaatgtcgg tatcatgact aaactcctcg ggagtatcaa ttgtatattc
     3301 attcagttca tagaatatgt attttgaaaa cgcatagaat tcttgaatat cttccggctg
     3361 gataaccctc aatataattt cgtttgcatg atttagttga tggatttgca tatttcaaca
     3421 taaaaaaaag ccggcaatta tgccggctta aatatactgt tttacctaca gttagaaatg
     3481 agaaataatg gcttcgccaa attctgagca tttcaataat gtagcacctt ccatcaaacg
     3541 gtggaaatcg tatgttactg tttttgcttc aatggctttt tccatacctt tcaggattaa
     3601 ttcggcaggt tcgagccatc ccataaattc aaacatcatc acaccactta aaataactga
     3661 acttggattc actttatcct ggttggcata ttttggtgct gttccatgcg tggcttcaaa
     3721 aattgctttt ccggtaagat agttgatatt agcacccggt gcaataccga ttcctcccac
     3781 taatgcagca agtgcatcag aaatataatc gccgtttaag tttaaagtag caatgacatc
     3841 atactcgctt ggtcgcaaaa taatttgttg caggaaatta tctgcaatag catctttcac
     3901 taacattttg cccgcagcca gagccgcttt ttgtgcttca tctgctgcta cttgtccgtc
     3961 ttctgccaat attttgtcgt attgtgccca agtaaacact tgatcaccaa actcacgctc
     4021 agctaattgg tatccccatt ctttaaattt accttctgta aatttcatga tattgccctt
     4081 gtgcaccaac gtcagggatt tgcgtttatg ttgtaaagca tattttaagg ctgcacgaat
     4141 cagacgctca gaaccttctt tagaaacagg cttgataccg atagatgatg tttccgggaa
     4201 acgtattttt ttaccgacat tcatatcgtt taacaagaaa ccaactaccc tgttacattc
     4261 ttccgtacct tccaaccatt caattccggc ataaatatct tccgtgtttt cgcggaaaat
     4321 aatcatatcc acctgatcgg gtactttaga gttagaggga actccttcaa aataacgaac
     4381 cggacgtaca caggcatata aatcgagtaa ctggcgaagt gccacattta acgagcgtat
     4441 acctccacct acgggtgttg ttaatgggcc ttttattgca ataagatgtt cgtctattga
     4501 tgttagcgtt tcatccggca accatgaacc agtttgttta taggcttttt cgccggccag
     4561 aacttctttc cagtgtattt ttctttttcc gccataagcc ttttctactg cggcatccat
     4621 cacacgcaca gatgctttcc atatatccgg cccggttcca tcgccttcaa taaatggaat
     4681 aatcggttcg tcaggtacat gcaagtgtcc atcgaccatt ttaatttttt ctgctgacat
     4741 atataataat tttaatttag ttgttaaaac aacgatttct tgaaaaatca ggacaaatat
     4801 acaaaaacta ttttcaataa ccttatttta acacaagtta tcataagcag gttgattttt
     4861 cagggtaaaa taccctagct atatgtttta gtttattata gttatacaat ttacttgttg
     4921 tgattatttc atttttctct taaattttac ttaggacgaa tgatgcttgc gatacaaaaa
     4981 acagaggtaa aaatttaaaa tacaggaatc ggcaaaggcg ttacgctgct atttaacaga
     5041 tttgcactca aaggctattt aagattaaaa atgctatgct tctgtgtggt tgaaatctca
     5101 caacggctta taaaaaatat tattacttac aacattttgc tacaaatatt atttataaaa
     5161 cacaggtagc cataatctac ggttgagata aagttgattt tcctcttaat aaaatagtaa
     5221 taccccgatt ctgcaaaaac ttccgatatt ggtttatgct aattatacct gcaattgata
     5281 ttatagacgg taaatgcgtc aggctcacaa aaggtgattt taatacgcaa gttacctata
     5341 acgaaaatcc tgtagaacaa gctttgttct ttgaagatgc cggattttct catttgcatt
     5401 tagttgattt ggatggagca aaacaaggca aaaccgtcaa ctggaatgta ttggaagaaa
     5461 ttgtaaaagc tacccgatta actattgact tttcgggtgg catcaaaacg aaagaacaag
     5521 ctcaaaaagc atttgataca ggcgctaatc agatttgtgt gggcagtctg gctgtaaaaa
     5581 gtcccaaaga atttatcgac tggatttggg cattcggtga ggaaaaactc attttgggag
     5641 ctgatgtttt gaacaataaa attgccatag aaggatgggc agccaccagt cagaaaacga
     5701 ttaccgaatt tctggattac tattttgaag aaggcataga ttatgtatta tgtacagatg
     5761 ttagtaaaga cggcatgatg atggggcctt cactcgaatt atatcgcacc attctttcag
     5821 catatgagga aataaaattg attgcatcag gaggcataac gactttagaa gacattctgc
     5881 aattacaagc attgggatgc tatggcgctg ttgttggcaa agccttgtac gaagctgaaa
     5941 cacgggaagc tattttaaac tatcaaaaaa acaagctgca tgctgactaa acgcatcata
     6001 ccctgcttgg acatcaaaga cggacatacg gtgaagggtg taaattttgt tgatttaatc
     6061 catgccggcg accctgtaga attagctgaa aaatatgtgg aacaaggtgc cgatgagttg
     6121 gtgtttttgg atatcactgc taccgttgaa aaccgaaaga ccttagttga attagtgagc
     6181 aggctctcag aaaaaatcaa tatccctttt accgttggtg gaggaatttc tacgttacaa
     6241 gatgtagatg ctttgctcaa tgccggtgct gataaagtgt ctgtaaattc tgctgcggta
     6301 aaaaatccgg atttaatcaa tgaacttgca gcccattttg gtactcaatg cattgtgctt
     6361 gctgttgaca caaagcaaga tgaagaatgg atggtttatg ttcatggtgg cagaacgcca
     6421 acgtcattaa aaacaaagga atgggtaaaa caagctcaag acagaggtgc cggtgaaatc
     6481 ttattaactt cgatgaacca tgacggcacg aaaaacggat ttgccttaga cattacctta
     6541 tggtgttcag aaaacctgca tattccagtg atagccagtg gtggcgcagg caatgaaaag
     6601 cattttgaag atgtattttt taaagctaaa gctgatgcgg cattagccgc cagtattttt
     6661 cattttggag aaataagtat cccacatcta aagaaatact tactagataa agacatctgt
     6721 gtccgtgttt aattaacaat ttattaggtc ttacaaccct gtactgtact taaaattggt
     6781 tacatttggc atcaattttt ttcaaatggc atcacctttt agttcgagta aagaacgacc
     6841 aaaaatatcc aaagattctc taaagaaaag tttaaaggtt tacagcttta tcaaacctta
     6901 tcagtattca ttttatctgg gcttgctctt tctcgtactt tcaagtctta cggtgcttat
     6961 tcttcctatg ctattgccca gattggcaga taccgcacaa ggtattccgg tacaattatt
     7021 taaaataaac ggcaaatatc tgattgattg caaaacgagt tgggatatcg taaaagtaac
     7081 tatggtattt atcgtattac aaggtgtgtt ttcatttttc agaatttaca tttttgccca
     7141 aaccaacgaa cgcgtgttga aagacattcg gtcttctgtt tataataaaa ctatcacatc
     7201 acccattatt ttctttgaga acaatagagt gggtgaactt atcagccgta tttccagtga
     7261 tatcaccaat gtacaagatg cactttcttt aaatattgct gaatttttac ggcaagtggt
     7321 aaccttatta gctggtattg tttatgtttt tgtttcctcc aaagaactta ctttttttat
     7381 tttatgcacg ataccggtgt tagttttatt ggccttattt ttttcaagat atattcgcaa
     7441 caatgcaagc agaacacagc aggctttagc gcagtcgaat ataatagtgg atgaaacgtt
     7501 tcacaatatc aatgtagtga aagcgtatgt caatgaatat tttgaaagca ataggtatgt
     7561 aaaaagtatt acagcagtaa aagatctggc cttgcgtgct gcgcttttcc gcggagcttt
     7621 tatttcattt cttatcgtgg gcgtaatgac cgtattaatt atggtattgt ataaagcgag
     7681 tatttatgtt cataattttt atttgcatgt tgagcccagt atgtcggttg ggcaactttt
     7741 gagttttatt ttctttaccg tatttattgg aggttcgcta gcaggaatgg gtgatttgta
     7801 tggcaaaatc atcagtgctt taggttcgtc agaccgtatt atttccattt tggatatgcc
     7861 atcagaaaca aaaatagaac aagtaccttt gcaaaaaata aatggagaaa tcgaatttaa
     7921 taaagtcaat ttttcttatc cttcaagaaa agaaaccgga gtactgcacg acatctccat
     7981 gcacatccgc aatggagaaa aagtagcctt agtcggctca tccggagcag gaaaatctac
     8041 catattacag ctgctgcttc gattttatcc ggttaatagt ggcgatatca aagtggcagg
     8101 aaaaaatatt tacgattatg atgttcaaga gttgcgcagc aatatcgcat tagttccgca
     8161 agaagtgatg ttgtttggtg gtactatccg ggagaacatt gcatacggca agacaaatgc
     8221 aacagatgaa gaaataaaac aagctactat aaaagcaaat gcatatgatt ttatccttaa
     8281 attccccgat ggttttgata ctattgttgg tgaaagagga gtaaaattat ccggcgggca
     8341 aaggcaacgt attgccattg ctcgtgctat attaaaagat cctgcaattt tattattaga
     8401 tgaagcgacc agtgcgctgg atgctgaatc ggaaaaatta gtacaagatg cattgaataa
     8461 attaatggaa aaccgaacga ctatcgttat tgcccatcgg ctttctacga tacgcaccgt
     8521 agataaaata tttgtcatga atcatggtgt cattgttgaa gagggtacac atgatgaact
     8581 cagcagtacg gaaaatggtc tttacagtaa tctgctgaaa ttacaatatc aaatagaata
     8641 atcgtatttt gcatattatt tcaaaaatgg cattaagact tgacatactg gcatttgcct
     8701 ctcatcctga tgatgtggaa ttatcttgca gtggcacatt aatgaagcat attgacaaag
     8761 gctacaaaat aggaattgta gatctgacag aaggcagcct gggcagcaga ggtactgttg
     8821 ccactcgcta caaggaggca aaaaaagctt ctgaaattat gggcattaca gtcagagaaa
     8881 atttgcaatt tagagacggt ttctttgaaa tcaatgaagc gcatctctta aaagtcatca
     8941 cggtgatacg caaataccaa ccggcaatta tattggcaaa tgcaatttat gacagacacc
     9001 ccgaccacaa acgcggggga gatttagttt caagagcaaa ttttttaagc ggtcttatca
     9061 aaatagaaac tgaagctaat ggaaaaaagc agcaattgtg gagagcaaag aaattattcc
     9121 gctacataca ggaaagttat ttaaaaccag attttgcgat agatatttca gcttatgctc
     9181 aaaaaaaact agcctgcata caagcatttt ccacacagtt ttacaatccg gaaagcacgg
     9241 aaatagaaac gccgatttca acaaaaaatt atctggatta tgtattatct aaagacaaag
     9301 tttttggcaa acaaattaat gtagactatg cagaaggctt tatttgtgaa caaccttcag
     9361 gaattgaaga tttgtttcat atgcaataaa taagaaatga aatccaaaag cttatttctt
     9421 ttctatggga ttatctcctt tctaatacta gccatttttt cattttattt tttcagagag
     9481 cgcattgcat ttactgactt aagctttcat ttatttacgc tcgtccgtac agcaaaattt
     9541 gccatattca attatcgtta tactgcagcc ttttctcaaa tgcttccact gtttgctata
     9601 aaatgtgaac agtccctgta cgtgatttca gcgttctatt cattttcatt tgtgttagtg
     9661 cagttttgtg tttttctggt aatcgttttc tatttcaaaa attatttttt ggcagcatgc
     9721 ttattgctgt tacaggtttt gcaaatacgc catacgttct actggaccgg ttctgaattt
     9781 atacccggag tggcattttt atttttgccc attgcttaca tagaaaaacg aacttcatca
     9841 gttagtaaaa ttcgtcacgc tgttatgctt attctatttc tgttgccggt cattttttct
     9901 catcctgtaa tcgtattccc atttttatac atagttgtat ttttcttcct tacgcaaaaa
     9961 ggaaagcgca aatcacttat ttggctcaca ctcttgtttt tgcttttatt tattgcaaag
    10021 tattttcttt tgaaaagcaa ttacgatgca ggagcattat catcgttaaa cccgaaaaag
    10081 ttgaaatggc atataatgct tcattatccg tcgcatcaat tattcatcaa taacctgtgg
    10141 aaacattaca acttgatggc agttatattt tgctttagct tgtttttgct ggcaataaaa
    10201 agaaattatc tgttagctgt tttattagtc acagtttgtc ttttatatat agcactagtc
    10261 aacttaaatt atccgcaggg tgctgaatta ttttatatcg aaaacttata ccttccgctt
    10321 tctgtcttta tcatattgcc tctggtttac agcttattta attacataaa aaacacaatg
    10381 cttatacatg catttttatt gttaatacta cttgtcggct tgatacaaat ttatcaaaca
    10441 catacaccct atacagcacg aataggatgg atgtatggta ttatggataa ggcaaaatct
    10501 gcaaatcaga ctaaaataat tttaccggta agagatgtac ccttagatac attgggtatg
    10561 gcttggggca gtccatatga gttttggatt ttatcaactt taaaatacaa gtcaacgacc
    10621 agtgttataa ttgaagaaac accacatgaa tacgactgga taaaagacag caagaacata
    10681 ttcatcacaa aatggggaaa ctttgcttat gaccaattaa accccaaata ttttgttttg
    10741 acagacagca gttcgtatca aatagtacaa gcaaagcatg agtaacggct ccatactttt
    10801 atttttttta gcattcgttt tttcaatttc taatattccg tttatattta ttgcatggaa
    10861 tccttttcta aaaaattatt ggaatggtat aatccgaaac tgcgcaattt accttggaaa
    10921 aacacgaaag atgcttataa aatatggcta agtgaaatca ttttgcagca aacacgagtt
    10981 gaacaaggaa cgccttacta tctggctttc ataaaaaaat atccgaaaat aaatttattg
    11041 gccaatgcac cattagatga agttctgaaa ttatgggaag ggttgggtta ttattcgaga
    11101 gcgagaaact tacatgcttc tgcacaacag gttttacatg aacataaagg taattttcca
    11161 aaaagttacg aagaacttaa aaaaataaaa ggtataggca actataccgc agcagctata
    11221 gcatcttttg cttatggact tccgcatgca gtagtagatg gcaacgtcta tcgggtctta
    11281 agcagagttt atggcatttt tactccaata gatacaaacc aaggcaaaaa acaatttgaa
    11341 actattgccc agaaattgct ggataaaaaa aatccgtctg tttacaatca ggctatcatg
    11401 gattttggct cattagtatg caaacctaaa aaccctttat gcaaagcttg cccttttcag
    11461 gatgaatgcc aagcattggc tatgcaaaaa atcggagaac ttccggtaaa atccaaaaag
    11521 acaagcatgc aaaagcgaga gttttatttt tttgtctata aaaacaaagg ctatacgtat
    11581 attgtacaac gtgatcagca agatatctgg aaagggttat atgaatttcc atttgtggaa
    11641 ataaaatcgg aaaaagataa atctcggttc aaaaaattaa agcctgttta caccgacaaa
    11701 cagcagcttt ctcatcaaat tatccatgct gatttttttg aaatagaaga aaaaacaaag
    11761 cttatgcaat tctttcctga tatacttcag tttaaaaaaa tcaaaataac caccctggaa
    11821 aactatgctt ttccgaaaat tatcagaaat tatttgcaac aagctaaatt atttctatct
    11881 tagtatggag gtttattgac taatatcaaa gaaaacaatt ttgatttaaa taggaatgcc
    11941 atgagtttta acaaagtttt tttaatcgga cgtttgggaa aagaccctga tgtacgcaaa
    12001 gtaaatgcaa caactacggt atgtaatttt ccgtttgcta caaatgaggt ttataaaaat
    12061 caggatggca gctatactga aacaacggaa tggcacaaca tcgtgatgtg gagaggaata
    12121 gcagaacgag ccgaaagaat tttgaaaaaa ggcagtcatg tttttattga aggcaaatta
    12181 cgcactcgct catgggatga caaggatggc aataaacgct acacgacaga gattgttgta
    12241 gaaaacttcc aactgcttga aaaacgtgag gcatccggac aaactactgg catcgttaac
    12301 tttggagatg cacaaaaaag cagtggtgat gatacgcatg ctgctcaaga tacaaatttg
    12361 accgaaccga cttttgatga tgatttacca ttttaattgt ttaatgtaat tgatgtattt
    12421 tggacgacgg catcccctta tgtagcttct tattaaatat tgagttttat tcatggaatg
    12481 agatatttaa tccatttaaa attcagtacg cagtatatgg tattgcaatt ttaatcttac
    12541 tctcatttac tgcaattctt tgtgcattag aagtagcgat atttatgttc agcaatgctg
    12601 aagtaaaaag actggaacag agcagtaata aaacggacgc attaatactg cattacattc
    12661 taaatcccaa cagatttatt gcaacttttg tattaacgac tactctcttt actctcgcta
    12721 tcgttttatt atttgaagcc ttactggaac aatggatcag agaagatttt atccatcagc
    12781 attcgacatt gttatttatt ataaaactat gctttgaaac atttattatc gtattatttg
    12841 ctgaagtaat tccaaaattg tatgccacac aaaaatacta tcaggtatgc agagccgtaa
    12901 tatacccaat taacatactg gataaaatat tttctccatt tgttactttg atgatgaaat
    12961 tttcagatgt aatagaaaag agattaatcg tagccaagca aacccttact gcacaagata
    13021 tacatgatgc attggaaatt actgaatcac aaagcacaga agtaagtaag gagaggacta
    13081 aaattttaaa aggtatcgta aaatttggca atcttaatgt gtcacaaatt atgcgctcgc
    13141 gcattgacat ggtggccata gatcatgatg ctaattttca cgatttgctt actattgtca
    13201 gagaatcagg ctattcgcgt attccggttt ttgaaaatga tatggataga attacgggta
    13261 tcatttatat caaagactta ttgaaatact tacatgagaa aaataatttt gactggaaaa
    13321 aactgatgcg ccccccttta tttgttcctg aaaataaaaa aatagatgat ttgctggaag
    13381 atattcaaga gcgacatatt catattgcca tcgtagtgga tgaatatggc agttgctgtg
    13441 gtttggtaac tctcgaagat gtactggaag aagtcattgg cgatattaag gatgagtttg
    13501 atgatgcgtc cgaaatagac ttcaaaaaat tagataataa taattacatt tttgaagcca
    13561 agacggcgtt gaatgacgta tgcaaagtga tggacttgcc aacggaaaca tttgatgaaa
    13621 taagaggcac tgcagattca ttagccggta tgttattgga actcaataat gaaattccaa
    13681 aagaaggtga cgaaataaaa tatggaaagt tcacgttcct tatcttagaa gctagtataa
    13741 cgcggattat tcgtgtgaaa atcagtatag cataaaattt catctctttt aatgtagatt
    13801 tgtagtatgg taaagaagaa acattttctg ctctttttaa taatggtgtt tattttggtt
    13861 tcgtgtaaaa cgaattacac gcccaaacag ccgggttatt tcagaataga ttttccggca
    13921 gaaagaaaat acaccgaata cagctgtgac gaatgtccgt ttacttttga atatcctgtc
    13981 attggtgcta tacaaaaaga ctctacggat atgcaggaaa tacataatca tccctgttgg
    14041 tttaatatta tttttccgga atacaaagca aaaatttatt taagctatac ggcaattaat
    14101 gccggcaacc ccttagacaa actcattgcc gattcttata aacttacttt caagcatacc
    14161 ttaaaagctg actatataga tgaatcccaa attacgggaa aaaatccgca tgtatccggc
    14221 atcttatatg atgtgggtgg tgatgctgcc agtggcgtgc aattttatgt aaccgacagt
    14281 acaaaaaatt tcatcagagg ttcactttat tttttctccc ctcctaatgc agattcatta
    14341 gcaccttgta ttcaatactt ccgtgaagat gttttgcatt ttgttgaaac cttaaaatgg
    14401 agataaaaaa cattaaatca tttcatccga tttcaacata aaaatggtag gtgcagatgc
    14461 tgaatatatc acaggcactt cttgctccgg cacaaaatcg tgcttcgatt taatgagcgt
    14521 ctttactata gttttgtctt gtaccttata ttcaattttg atatggtctg cttttttctc
    14581 tttcttttca attgctctta cccgagcatt taccacaatt ccatcttttt ttatttttga
    14641 ctgctgataa agttgttgtc cataatattg caacaccaca taaaccagac agataaaagc
    14701 acttaaatag atgttcagct tttcatccac ccattgataa atccatgaat aaagcatgtc
    14761 tttcccggct gaagcatccg gaataaaata atgcatctgc cagatagtaa ttaaagccac
    14821 tatccctata agctgaaaag caattgccgt gtattcccat acctccgaac gatgtgcttg
    14881 tgtaataaac aaataaatac cggcaagaat aagtacgact gcaaatatca ggatgaatag
    14941 ttgcatgatt atggtttaaa ttgcctgact aaattttggt gcatcctgct tttgacgatc
    15001 gtcattcatg cgtttatcaa aaacagcaca gacattgcgc aaaaatcccc aacccatatg
    15061 tgtgagctta agttcgccgg gttgccattg caaaatacca tctgcctcca aatcattcag
    15121 cttttccatt ccactatctg ttattacgac agactcatcc catttcgttt cgcctttgca
    15181 ggatatattt agtatctgct gtctgtaaa
//
LOCUS       DAOQII010000029        15163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1871_length_15163_cov_5.507744, whole genome shotgun sequence.
ACCESSION   DAOQII010000029 DAOQII010000000
VERSION     DAOQII010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1871_length_15163_cov_5.507744"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLF48_02575"
     CDS             complement(<1..351)
                     /locus_tag="PLF48_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNF48237.1"
                     /translation="MQHIRNFCIIAHIDHGKSTLADRLLEVTKTVSDRDLQAQTLDDM
                     DLERERGITIKSHAIQMDYLHTDGQKYTLNLIDTPGHVDFSYEVSRSLAACEGALLLV
                     DAAQGIQAQTISNLY"
     gene            complement(521..>1641)
                     /locus_tag="PLF48_02580"
     CDS             complement(521..>1641)
                     /locus_tag="PLF48_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF48238.1"
                     /translation="LSSSSNAQPNASQPNTYTVTATNTTNGCTATDQVVISQNITPPV
                     ANAGVDKTLTCTTTSVILGTTAQANTTYSWSPATGLSGTAIAQPNASQPNTYTLTATN
                     TTNGCTKSDVVIVSQNITPPPANAGADKIITCAQPKVSIGVSTGNFTYSWSPSTGLTS
                     PNTAVTLSDSVGTFGLTVTNPVNGCFAVDSVTITIDTIQPVADAGEDIQFTCPISPVT
                     IGSPDNPILRYVWNNTYGISATNIAQPTADTAGIFVVRVQSTINGCWSKTDSVTVYWK
                     NCACDVFVPDAFSPNGDGVNDVVKPLKNCDDISNLMFRIFNRWGEQVYQSESFDEGWN
                     GFFKNEAQQMDSYIWTISYHDVLYNKDVFLKGVVTLVR"
     assembly_gap    1642..1741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1742..3859)
                     /locus_tag="PLF48_02585"
     CDS             complement(<1742..3859)
                     /locus_tag="PLF48_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48239.1"
                     /translation="MVKILRTFLLGISVLLTLNINAQATYIRPCYSINAGLDSSICAI
                     NCVTINRTAIPQYLNKATNTYVVQQMTMPSPLYNNTGTNVIVNTDDVWSGIVNIGFNF
                     CYFGNTYNKLVIGANGIISFNTAYANAYCPWSYTGGIPDATKPINSIMGVYTDVDPSV
                     GFNANRINYYTEGVAPCRRFVINYYRVPMYSCNALLYTTQIVLHEAYNIIDVIVVNKP
                     VCSSWNGGRGIIGIQNAAGNTAFSAPGNNPSTSAISNQCWRFIPNGALMQSVSWYQGA
                     TQVSNTDVLSASLCPTSSTVFETRLLNTRCDGSSVTVTDTVRFNVNLGGANAGVDKTL
                     SCITPNITIGTPAQPNTNYTWTPGAGLSNPNIAQPSTNTPNTYILTATNTITGCSAKD
                     TVIVGPKTFPVADAGPNKTITCANPSIQIGTTYVPGIFYAWSPATGLSSTSIAQPLAS
                     QPRMYYLTVTDSATGCTGTDSVMVTIDTIRPTADAGLDKSISCTFPNAQIGTSAIAGN
                     SYTWLPPTGLSNPNIAQPIASSGGTYMLTVTKNSNGCTATDNVLITADTIKPTANAGL
                     DDTLTCSVPSIVLGTTAVAGNSYVWSPPLGLNNPNIAQPTASQPNTYTLTVTKNANGC
                     TATDAVTILRDTVKPIANAGRDSLLTCAVLSIPIGTTAVPGMNYTWSPSGGLSSSSSA
                     QPNASLPNTYTVTVTNPVNGCSKS"
     gene            complement(4018..5631)
                     /locus_tag="PLF48_02590"
     CDS             complement(4018..5631)
                     /locus_tag="PLF48_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF48240.1"
                     /translation="MRKQTIILALSIISMLNACKPKQKLQEMTAVEEETFQDIQLAAP
                     KADALSYPECCKISETNYAEFNTEQYDYQQENGFKTAKENPLSTFSIDVDRASYSNIR
                     RILNSGKLPPKGAVRIEEMINYFTYNYPKPVEQPFSITTEYGSCPWNKEHQMVLIGLQ
                     GKAYEEEITPPYNLTFLIDVSGSMEAADKLPLVKKALKLLTARMRVKDCISIVVYAGN
                     AGLVLAPTYGTEKDKIYNAIDELSSGGSTAGGEGIKLAYKTANLNFSKSKINRVILAT
                     DGDFNVGVSSDDELVGLIEKERATGIFLTVLGFGSGNYKDSKMEKLADKGNGNYAYID
                     NEQEAKKVLITEIGGTLQTIAKDVKIQVEFNPENVKAYRLIGYENRVLNNEDFNDDTK
                     DAGELGAGHCVTALYEIIPAGSTEQVPQSDPLKYQQATIQASKLNEHELLTVKFRFKK
                     PDSDEKSILMQQIVHTSKLNKTTSANFLWATTVASFGMLLSHSDYAGILSYNSLKSYL
                     KQINIADLDENKLEMINLIEKAEQLGKND"
     gene            5796..6413
                     /gene="scpB"
                     /locus_tag="PLF48_02595"
     CDS             5796..6413
                     /gene="scpB"
                     /locus_tag="PLF48_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNF48241.1"
                     /translation="MEDVQKNIEAIIFSAEAPVTAEELLKHLNKDNDTPLNTDEVEGI
                     IQVIKDKYANDLFPFEIGETGGGYQFLTKADYHIVVSSYLNRNTVKRLTTAALETLAI
                     IAYKQPVTKNIIETIRGVNADYTIQKLMEKELIEIAGRSEEPGKPLLYKTTRQFLDYF
                     GINALDELPKLKEFEELQNQIGEKENIETENRTSVGSTDSVASQN"
     gene            6340..7266
                     /locus_tag="PLF48_02600"
     CDS             6340..7266
                     /locus_tag="PLF48_02600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF48242.1"
                     /translation="MRKRISRPKIERLSDQPTPSHHKTSFQKPKKFIKRPNNDPFAQK
                     IEQTVSGFVPPKDHHADTEEIRLNKYISNAGVCSRRQADDYIKEGYVTVNGETILEMG
                     YKVQAKDDVRFKGKKVENERKVYILMNKPKGLLTTTADEHNRKTVMDLVSTVKNARIY
                     PVGRLDRNTTGVLIMTNDGELAQKLSHPSGNITKVYQVELNIPLKPEDLLKIKKGVQL
                     EDGLAEVDEIDYTLPRGDDRHVGVELHSGKNRIVRRIFEELGYEVVKLDRVLYGGLTK
                     KNLPRGKWRFLTDKEIIFLKYFVGNSKPKKQA"
     gene            7272..8159
                     /locus_tag="PLF48_02605"
     CDS             7272..8159
                     /locus_tag="PLF48_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HNF48243.1"
                     /translation="MKFAAIDIGSNAVRLVFVNVYETPHGTQYVKDIMYRVALRLGEE
                     VFSEGKITKQKEKEFISTMKAFKHLIRVHKPVQVMACATSAMRDAKNSKEIVDKIFRK
                     TGIEIQVISGKKEAQYILSNHVERFSKENSINYLYIDVGGGSTELIFLSNGKLIDKWS
                     FNIGTLRMAMGKVSEKNWKELEVWLEHFKGKYAEIVGIGSGGNINSILNHFVKNQKDR
                     VDYKDLEKALNTLEPMSLDERILKYAFRPDRADVIVPATKIFLFIMKRLNIDHLIVPK
                     VGLGDGMIHEMYEKMVRVK"
     gene            complement(8169..8414)
                     /locus_tag="PLF48_02610"
     CDS             complement(8169..8414)
                     /locus_tag="PLF48_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNF48244.1"
                     /translation="MPKKNCILHLNLAPYSDNIQFVKENNKKTGGDIKIHGYDRIWTG
                     KFHRFYDCSIGCICVTNTEIKELFDYVQFGTPIEILP"
     gene            8957..9124
                     /locus_tag="PLF48_02615"
     CDS             8957..9124
                     /locus_tag="PLF48_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48245.1"
                     /translation="MYHTIKEFTEEYKMECQSTLKLLNVLTTESLYKTVDGKVRSIGR
                     LAYHIAHTVSG"
     gene            9121..9462
                     /locus_tag="PLF48_02620"
     CDS             9121..9462
                     /locus_tag="PLF48_02620"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF48246.1"
                     /translation="MMSKTGLDIPMEIKNPAEDTSITAAQLATAYTQAAATLLAEMEA
                     KWTDASLQDSNNLYGMEWSNALTLSILLKHEIHHRGQLTVLMRLAGLIVPGVYGPSAE
                     EWEQYGMKMEE"
     gene            9515..10171
                     /locus_tag="PLF48_02625"
     CDS             9515..10171
                     /locus_tag="PLF48_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48247.1"
                     /translation="MMYNFYTNKQTVYYHLLKKKYSKSSRTCNEYFSRFKSIRNQIQK
                     ISVYVFSSIIFCSCLHSKRASYEELEHNLISNFEDNKCIFDSIRNIGQHLDSIQYISL
                     NNENVEFWILDTTSNIQTIQNNINKKYSILNILKIDSINEIEFSDLCKYLKMVDCNSL
                     QIMPNTENINNKINHPIELVYKEWNGILVCYKIFPRALYEHEIKWFKKYLVDKKKEES
                     "
     gene            complement(10287..12506)
                     /locus_tag="PLF48_02630"
     CDS             complement(10287..12506)
                     /locus_tag="PLF48_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNF48248.1"
                     /translation="MEINAEQEKAAILKDYRQLLRLAQPRMQKGDNKRLRLAFEMAAD
                     AHRHMRRKSGEPYIHHPIAVAKIVSGDIGLGTTSVIGALLHDTVEDTDLTLDDIKKEF
                     GATVSKIIDGLTKISKISHQTESIQAENYRKILLTLSDDIRVILIKIADRLHNMRTLD
                     GQSRKNQLKISSETIYLYAPLAHRLGLYNIKGELEDLAMKYTETEQYKEIAKGLEATK
                     KDRTKYINDFIRPLQEELNKAGLHAHVFGRPKSISSIYNKIKTKGVSFEDVYDKFAIR
                     VILDSPPEKEKEDCWRAYSIVTDKYSPSPNRLRDWISHPKANGYESLHTTVMGPGGQW
                     VEVQIRSKRMDEVAEKGFAAHFKYKENKFKPTNNAEEAIESWLMQVRDFLKNPDSNPI
                     EFINDFKLQLYHKEIYTFTPKGEMKILPAGATALDFAYGVHTNVGDTCIGAKVNGKLV
                     SISHVLHNGDQIEIITSKKQKPNEDWLNIVTTSRAKSKIKNSLREEKKKIAEDGKEIL
                     QRKFNQLKLTYDNENINFLMHVYKQPSSLDLMYAIAKNNINLSELKQYKNAGGHLEVE
                     RQEKPAKEKQEYEYQPEKEISGASELVIFEQSGSQFPYEFAKCCNPIPGDDVMGFISI
                     NGMIKIHRTGCNNIINLASKYGYRIIKTRWTSKTTPTFLTTLKMKGLDDMGFINKLTN
                     IISTKMKLNMQSISIETNEGIFEGVVKVYVKDNAQVSDLIRNIKSIEGINSVIREEI"
     gene            complement(12572..13888)
                     /locus_tag="PLF48_02635"
     CDS             complement(12572..13888)
                     /locus_tag="PLF48_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48249.1"
                     /translation="MKFLADSSIYKDAEWQLINYIIICLQSKVMDENDLEKKIIFAKL
                     FPGELYNEHKINKLISDCNKSVEMFIIAEYVEQRQFLQKMALIKFYQRKKMNRYFESE
                     IKQTAQYLLKTKESLQKLNDKVQLNEIINEQNSQSNFRQANYIEFLDSLSSFNKLAET
                     KYKNAILINNYEYVEIDVNNNILFDLHHQINKLLQNNLSVEDFHRCFDLLISHSEQIE
                     GYELKDCVIILISIAIKQVHQNIDGAQLRLFELYKFLIDNNLILEEDKTITSAIYKNV
                     TTIGLYLQKYDFVEQFVEEYKYNLNEHEQEDVYSYNKAHLLYYKKEYELVLQLISTTK
                     YKDVFYRLSSRVLLIKTYFELEKSDETYFDLLANSVNAFKKYVYTNEEINDYYKTRYK
                     NFIKYVLKLTQLNRDKIQIQKFIDELISNKDIIEYAWLLESANKLL"
     gene            13982..14650
                     /locus_tag="PLF48_02640"
     CDS             13982..14650
                     /locus_tag="PLF48_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48250.1"
                     /translation="MKRILLSFLVFTLFLACKKNKTETIPFKFTKISGNSTLSSKGIL
                     GLEWVDNENTSWNILLRNLNTGVLTTRTTTNHNLTDSIVLTDNYRITVTGNKIPVDTT
                     NPCNTGSVNARIGNMPDLFTTFNPCPPFSITNVFAEKVNATTATSTLAWSDANNTSWK
                     VKITNLTTAVSNTTSVAVTDLTLNFALDQPQLIEISGDQNGAKKSVNVQVNNDATSIV
                     SNFY"
     gene            14964..>15163
                     /locus_tag="PLF48_02645"
     CDS             14964..>15163
                     /locus_tag="PLF48_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48251.1"
                     /translation="MKTSIKKQIAIALVAIFSIVSLNSCKKNETTINRPTATEFNALQ
                     DAALNSLKETHTFLAEAGTTFTS"
BASE COUNT         4741 a         2693 c         2694 g         4935 t
ORIGIN      
        1 atataagttt gaaatagttt gagcttgaat gccttgtgct gcatcaacaa gtaataatgc
       61 accttcgcat gcggccaaag aacgggatac ttcataggaa aagtctacat gccctggggt
      121 gtcaattaag ttgagtgtat acttttgccc atccgtatgc agatagtcca tttgaatagc
      181 atggctcttt atcgtaattc cacgttctcg ctccagatcc atatcgtcta aggtttgagc
      241 ttgtaaatca cggtcggaaa cagtcttcgt tacttccaaa agtcggtcgg ccaatgtgct
      301 ttttccatgg tcaatatggg cgatgataca aaaattccgt atatgttgca tggcgcaaaa
      361 ataacacttt tttagaggat gtgtatagaa tgtaaatgta gatgtagatg tatatcagag
      421 ataaataaac aaacatactt gcggtctttg caattaagtt tgaatcaaaa atataaaatg
      481 aaagtgaaat ttatactctt tgctgaattt tattattaaa ctatctcacc aaagtgacta
      541 cccctttcag gaatacatct ttattgtaga gtacatcgtg ataagatatt gtccaaatat
      601 aagagtccat ctgctgtgct tcatttttaa agaatccatt ccacccttca tcaaaacttt
      661 cggactgata cacttgttca ccccatcggt tgaaaattct aaacattaaa ttagaaatat
      721 catcacaatt tttaagcggt ttaacaacat catttacccc atcgccatta ggagaaaagg
      781 catcaggaac aaaaacatcg caagcacaat ttttccaata taccgttaca ctgtcggttt
      841 tagaccagca tccgtttatt gtactttgta ctctaacaac aaatattcca gccgtatctg
      901 ctgtcggttg ggcaatattg gttgcactga taccataagt attattccat acgtaacgca
      961 atatcggatt atctggagaa ccgatagtca caggcgaaat tggacaggta aattgaatat
     1021 cctctccggc atccgcaaca ggctgaatgg tgtcaatagt aatggtcacc gaatcaactg
     1081 caaaacaacc atttaccgga ttcgttaccg ttaaaccgaa cgtacccaca gaatcagata
     1141 aagtaactgc agtattgggt gatgtcaatc cagtgcttgg cgaccatgaa taagtaaagt
     1201 ttcctgtaga aacaccaatg cttactttag gttgtgcaca ggtaataatt ttatctgctc
     1261 ccgcatttgc aggtggtggt gttatgtttt gtgaaacaat aactacatct gattttgtac
     1321 atccattcgt agtattcgtt gcagtaaggg tataagtatt cggttgtgat gcgttcggtt
     1381 gagctattgc tgtaccggat agtcccgttg caggcgacca agaataagtt gtattagcct
     1441 gagcagttgt accaagaatt acagaggttg tggtacatgt cagcgtctta tctactcctg
     1501 catttgccac aggaggagta atattttgtg aaataactac ttgatccgta gccgtacaac
     1561 cattggtggt atttgtagcg gtaaccgtat aggtattcgg ttgcgatgca ttcggttgag
     1621 cgtttgaaga actagacaac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 ntgatttaga acaaccattc accggattgg ttacggtaac agtataggta ttcggtagag
     1801 aagcatttgg ttgagccgat gaagaactgg acaaaccacc actaggcgac caagtataat
     1861 tcattcccgg cacagcggtt gtacctattg gtattgaaag tacagcacat gtcaacaagg
     1921 agtctctacc tgcattggca ataggtttaa cggtatctct caatatagta acagcatctg
     1981 tagccgtaca accgttggcg ttttttgtaa ctgttaaggt ataagtattg ggttgcgaag
     2041 ccgtaggctg agcaatatta ggattgttta atcctaatgg aggtgaccaa acataactgt
     2101 tgcctgcaac agccgtagtt cccaatacaa tactcggcac agagcaagtt aaggtatcat
     2161 ccaagcctgc attagccgtt ggcttaatag tatctgcagt aattaataca ttatcagtag
     2221 ccgtacaacc atttgaattt tttgtaacgg ttagcatata tgtcccaccc gaactggcaa
     2281 ttggctgagc tatatttgga ttactgagtc cggtaggtgg cagccatgta tacgaatttc
     2341 cagcaatagc agatgtgcct atttgtgcat ttggaaaagt acaagaaata gatttatcca
     2401 aacctgcatc ggcagttggt cttattgtat ctattgtaac cattacagaa tccgtacccg
     2461 tgcatcccgt agcagaatct gtaacagtga gataatacat acgtggctgt gaagccaaag
     2521 gttgagctat tgaagtagaa gatagaccgg tagccggtga ccatgcatag aatattccgg
     2581 gtacataagt tgtgccaatc tgtatagatg gattagcaca agttatcgtt ttattcggac
     2641 ccgcatctgc taccggaaaa gtttttggcc caacaattac tgtgtctttt gctgaacaac
     2701 ctgtaatagt gtttgtagct gttagtatat acgtattagg tgtattggtt gatggttgag
     2761 caatgttagg attactaagc cctgcacctg gagtccaagt gtaattcgta ttcggttgag
     2821 ccggggttcc aatagttata tttggtgtaa tacaactaag tgttttatct actccggcat
     2881 tagcccctcc caaattaaca ttaaatctga cagtatctgt aacagttaca gaagaaccat
     2941 cacatctggt gtttaaaaga cgagtttcaa atacagtaga tgatgtggga cataaactgg
     3001 ctgaaagtac atcagtattg gaaacctgtg ttgccccttg ataccatgat acactttgca
     3061 ttagtgctcc attagggatg aagcgccagc attggttaga aattgctgag gtagaggggt
     3121 tattgccagg ggctgaaaat gcagtattgc ctgctgcatt ttgtatacca ataattcctc
     3181 gaccaccatt ccaactggaa caaacgggtt tgtttacaac gattacatca ataatattat
     3241 acgcttcatg taagacgatt tgagtggtgt ataagagtgc gttacagcta tacattggta
     3301 ctctatagta attaattaca aatcttctgc aaggtgcaac accttctgta taataattga
     3361 tgcggtttgc attgaatcca acactggggt ctacatcggt atatactccc attatcgaat
     3421 ttataggttt tgtggcatca ggaatacccc cggtataaga ccaaggacaa tatgcattag
     3481 cataggctgt attaaatgaa ataataccat tggcaccaat aactaattta ttatatgtgt
     3541 ttccaaaata acaaaaatta aacccaatat taactattcc actccataca tcatcggtat
     3601 ttacaatgac atttgtaccc gtattgttat ataagggact gggcatagtc atttgttgaa
     3661 caacatatgt atttgtcgct ttatttagat attgtggaat tgcagttctg ttaatcgtaa
     3721 cacagttaat agcacaaatg gatgagtcta aacctgcatt aatgctataa catgggcgaa
     3781 tataagttgc ttgagcattt atatttaatg taagcaatac ggaaatacct aataagaaag
     3841 ttcttaaaat ctttaccatt ctaaaagttt attaattagt tgagtgagtt ttaagaacct
     3901 aatatagtat atttatcaca aaagacttaa tttttgttaa ttaaaaagga ggatttcagg
     3961 tttttttact gaaatactcc cattagtgat gtaatatgat gtgtttattt ggaaatttta
     4021 gtcattcttt cctaattgtt cagccttttc aatcaaattt atcatttcca gtttgttttc
     4081 atccaaatct gcaatattaa tttgttttag atatgatttt agtgaattat aactgagtat
     4141 gcccgcataa tctgaatgtg acaacagcat cccaaaactt gctactgtag ttgcccataa
     4201 aaaattagca gaagttgtct tgtttaattt tgaagtatgc actatctgtt gcataagtat
     4261 gcttttttca tctgaatccg gttttttaaa tctgaatttt acagttaata actcatgctc
     4321 atttaattta cttgcctgaa ttgtagcctg ctggtatttt aaggggtctg attgaggaac
     4381 ctgttcagta cttccagcag gaattatttc gtaaagcgca gtaacacaat gccctgctcc
     4441 taattctcct gcatccttgg tgtcatcatt aaaatcttca ttatttaaaa ctctgttttc
     4501 ataaccaata agtcgatagg ctttaacatt ttcgggatta aattcaactt gaatttttac
     4561 atcttttgct atagtttgca atgttcctcc tatttcagtt atcagcactt tctttgcctc
     4621 ttgctcgttg tctatatagg cataattacc attgccttta tctgccagtt tttccatctt
     4681 tgagtctttg taatttccac ttccaaaacc taaaactgtg aggaatatgc ctgttgctcg
     4741 ttctttttct attagtccca caagctcatc atctgagcta acgcctacat taaagtctcc
     4801 atctgttgca agaattacgc gattaatctt cgatttagaa aaattcaaat ttgccgtttt
     4861 ataggctaat tttattccct cgccaccagc tgtcgaacct cctgaactta attcgtcaat
     4921 ggcattataa attttatctt tttcagtgcc ataagtagga gctaaaacca atcccgcatt
     4981 tccggcatat actactattg atatacaatc tttaacccgc attctagccg ttaatagttt
     5041 aagagctttt tttaccaaag gtagtttgtc tgcagcttcc atcgatccgg atacatcaat
     5101 caaaaatgtt agattgtaag gaggagtaat ttcttcctca taagctttcc cttgtaagcc
     5161 aataagtacc atctgatgtt ccttattcca cggacaactg ccatactctg tggttatgga
     5221 gaagggttgt tctacaggtt taggatagtt gtaagtaaaa taatttatca tttcttctat
     5281 gcgtactgca cctttagggg gcaatttccc actgtttaaa atgcgcctga tattagaata
     5341 agaagctcta tctacatcaa tactaaaggt agataatgga ttttctttgg cagttttaaa
     5401 accgttttct tgttggtagt cgtactgttc tgtattaaat tcagcataat ttgtctcact
     5461 gattttacaa cattcaggat aggataatgc atcagccttt ggagctgcta actgaatatc
     5521 ttgaaatgtt tcttcctcaa cagctgtcat ttcttgtaat ttctgtttag gtttgcaggc
     5581 attcagcatg cttattatgc tcaatgccaa aataatggtt tgttttctca tgttggtaag
     5641 tttaattaaa ttttaaacgc aacaatgttg catttatttt tttatgaaaa taggttttaa
     5701 caattatttg cattcgtacc caataaatac agattgaaag agaggtgacc ctttatttaa
     5761 tttataattt cagctttatt attatctttg cccccgtgga agatgtacag aaaaatattg
     5821 aagctattat tttctcagcg gaagctccgg ttacggcaga agaactattg aagcatttga
     5881 ataaagataa cgatactccg ctcaacacgg atgaggtaga aggtattatt caagtgatta
     5941 aagataaata tgccaatgac ttgtttccat tcgaaatcgg agaaacggga ggcggttatc
     6001 agtttttgac aaaagccgat tatcatatag ttgtgtcttc ttacctgaac cgcaatactg
     6061 taaaaagact tacaacggct gctttagaaa cattagctat tattgcctac aagcaacccg
     6121 ttaccaaaaa tattatagaa accattcgcg gcgtcaatgc tgattatacc atccaaaaac
     6181 tgatggagaa agaattgatt gaaatagcag gacgcagtga agaacccgga aaaccgttgt
     6241 tgtataaaac aacccgacaa tttttagact atttcggcat aaatgcttta gacgaactgc
     6301 cgaaattaaa agaatttgaa gaacttcaaa atcaaatagg tgagaaagag aatatcgaga
     6361 ccgaaaatag aacgtctgtc ggatcaaccg actccgtcgc atcacaaaac tagtttccag
     6421 aaaccgaaaa agtttatcaa gcgaccaaac aatgatccgt ttgcccaaaa aattgagcaa
     6481 accgtaagtg gttttgtgcc acccaaagat catcatgccg atacagaaga aataagacta
     6541 aataagtaca tttccaatgc aggggtctgt tcccgtcgtc aggctgatga ttacatcaaa
     6601 gaaggctatg taactgttaa cggagaaacc attctcgaaa tgggttacaa ggtgcaagct
     6661 aaagatgatg tacgttttaa aggcaaaaaa gtggaaaatg agcgcaaggt ctatattctg
     6721 atgaataaac ctaagggctt gcttactact acagccgatg agcataaccg caaaacagtg
     6781 atggatttgg ttagcactgt aaaaaacgca cgaatatacc cggtaggtcg tttagaccgt
     6841 aacaccacgg gagttttgat catgaccaat gacggcgagt tggctcaaaa actgtcgcat
     6901 ccaagtggta atattactaa ggtataccag gtggaattaa atattccgct gaagcccgaa
     6961 gatttattaa aaataaaaaa aggtgtgcag ctggaagatg gtctggccga agtggacgaa
     7021 atagattata ctttgcccag aggtgatgac cgacatgtcg gcgttgaatt gcacagcgga
     7081 aaaaaccgta ttgttcgtcg tatttttgaa gagcttggct atgaagttgt gaaattagac
     7141 agagtacttt atggtgggtt aactaaaaag aatttaccaa gagggaaatg gcgttttttg
     7201 accgataaag aaattatctt tttgaaatat tttgtaggta attcaaaacc aaaaaaacaa
     7261 gcttaagcat tatgaaattt gccgctatag atattggttc caatgcagtt cgtctggtgt
     7321 ttgtcaatgt gtatgaaacc ccgcacggaa cacaatatgt aaaagatatt atgtatcggg
     7381 tggctcttcg tttaggagaa gaggttttca gtgaaggcaa aattaccaaa cagaaagaaa
     7441 aagaattcat cagcacgatg aaagctttta agcacctgat tcgtgtgcac aaacccgtgc
     7501 aggtaatggc ttgtgccaca tctgccatgc gtgatgcaaa aaacagtaag gaaatcgttg
     7561 acaagatctt tcgcaaaacg ggcattgaaa ttcaggttat atccggtaaa aaagaggcgc
     7621 aatatatttt atccaaccat gtggaacggt tctcaaaaga aaattctatc aactatttat
     7681 atattgatgt aggtggagga agtacggagc tcatcttttt gtcaaacggc aagctgattg
     7741 ataaatggtc ttttaatatc ggtactttga ggatggcaat gggaaaagtg agcgaaaaaa
     7801 attggaaaga actcgaagtc tggttagagc attttaaagg aaaatatgca gaaattgtcg
     7861 gcatcggttc gggagggaat atcaactcta ttttgaatca ttttgtcaaa aaccaaaaag
     7921 accgtgtgga ttacaaggat ttggaaaaag cacttaacac acttgagccc atgtctttag
     7981 atgaacgcat acttaaatat gcattccgcc cagacagagc agatgtaatt gttccggcaa
     8041 caaaaatttt cttatttatc atgaaacgat taaacatcga ccacctcatt gtacctaaag
     8101 ttggtctggg tgatggtatg atacatgaga tgtatgagaa gatggtgcgt gttaaataaa
     8161 attgattttc atggaagaat ttcgataggg gttccaaact gtacataatc aaacaattct
     8221 tttatttctg tatttgtaac acaaatacat ccaatgctac agtcataaaa tctatgaaac
     8281 ttaccagtcc agattcggtc atatccatga atctttatat ccccacctgt ttttttattg
     8341 ttttctttta caaattgaat attatctgaa tagggagcta aattcaagtg taaaatgcaa
     8401 ttttttttgg gcataagacg acttttttca aaacatggat gaggttatct tccctgctct
     8461 taatggatgg tataatattg tactttcgcc aaaaaaatac aatatgaaac acctaatcgt
     8521 taaacagata tactaaatag aagctgtgaa tgtgcaatat cctcgtgtta cccaaaagca
     8581 gatttcaaaa caactcggta tcagcgaatc acagttaagc agagaattga aacgcaatat
     8641 gaagtaaaat ggcaaacaca aaaaagctaa gtagtggcga tgcctttcat tagtggatta
     8701 gttaattagt ttaatagttt agtagtggta caaattttat tcttatcaga aaaccacgta
     8761 gtggcatttt ccgcatacat agtcttaatt tccttaatgc cttaatggta aataattcgt
     8821 taataaaaaa aacacgtagt gtcgttaact ttttagatag tttaatagtt gggtagtttg
     8881 atagtttagt agtggtacat aatcatagtt ttctttttat cttgtttgct tattatcttt
     8941 tacaaaatct aacaacatgt atcatacgat taaagaattt actgaggagt acaaaatgga
     9001 atgtcagtcc accctaaaac tgttgaacgt gcttactacc gaaagtctat acaaaaccgt
     9061 tgacggaaag gtgcgttcta ttggcagact tgcttatcat attgcgcata ccgtttcggg
     9121 atgatgagta aaacaggctt ggacattccg atggagataa agaacccagc agaagacacg
     9181 agcattacag cggcacaact cgccactgca tatacacaag ccgcagctac tttattggca
     9241 gaaatggaag ccaagtggac agatgcctct ttgcaagact ccaataattt gtacggcatg
     9301 gaatggtcga atgcgcttac cctttccatt ctgttaaagc acgaaataca ccaccgcggg
     9361 cagcttaccg ttttgatgcg attagccgga ttaattgttc ctggagtata tggaccaagt
     9421 gccgaagagt gggagcagta tggaatgaaa atggaagagt aaagagtaat ttattctgac
     9481 caaaaagctt tctgtttcaa ataaaaagtt ctcaatgatg tacaactttt atacaaataa
     9541 acagactgtt tattatcatc ttctaaaaaa gaagtattct aaaagttcgc gtacctgtaa
     9601 tgaatatttt tcaagattta agtctattag aaaccaaatt caaaagattt ctgtttatgt
     9661 tttcagctca ataatttttt gtagttgctt acattctaag agggcaagtt atgaagaatt
     9721 agaacataat ttaatctcaa attttgaaga caataaatgc atctttgatt ctatcagaaa
     9781 cataggacaa catttagata gtattcaata cattagttta aataatgaaa atgttgaatt
     9841 ttggatactt gatacaactt caaatattca aacaatacag aataatatca acaaaaaata
     9901 cagcatctta aatattttaa agatagacag tataaacgaa attgaatttt cagatttatg
     9961 taaatattta aaaatggtgg attgtaattc cttacagatt atgcctaata ctgaaaatat
    10021 aaataataaa atcaatcatc caatagaatt agtttataaa gaatggaatg gcattttagt
    10081 ttgttataaa atttttccta gagctttata tgaacacgaa attaaatggt ttaaaaaata
    10141 tttagtagat aaaaaaaagg aggaatctta aataaataca ctatctggtt tttaaaaata
    10201 gattaaaatt gcttctacac atatctatca atacatctcc aacagaatct tacctcttgc
    10261 acaagatatt ttttttaaaa actgaattaa atttcttcgc gtatgacgga gttaatgcct
    10321 tctattgatt tgatattccg aatcaaatct gaaacttgcg cattgtcttt tacatatacc
    10381 ttcactacgc cctcaaaaat accttcgttc gtctctatag aaatagactg catattgagt
    10441 ttcatctttg tagatataat attagtcagc ttattgataa aacccatatc gtcgagtcct
    10501 ttcatcttta gggtagttaa aaatgtaggt gtcgtcttgg atgtccatcg cgttttgata
    10561 atgcggtagc catatttaga tgctaaattg atgatattat tgcatcccgt acggtgaatt
    10621 tttatcatcc catttataga gataaaaccc atcacatcgt ctcctggaat cgggttacag
    10681 catttggcaa actcatacgg aaactgactg cccgactgct caaaaataac caactccgaa
    10741 gccccggaaa tttctttctc cggctgatat tcgtattctt gtttttcttt tgcaggcttc
    10801 tcttgtcgct caacttctaa atgtcctcca gcatttttgt actgttttaa ttcgcttaaa
    10861 ttgatattgt tttttgcaat ggcatacatc aaatccagcg aactcggctg cttgtaaaca
    10921 tgcataagaa agttgatatt ttcattatca tacgtgagtt tgagctggtt gaatttgcgc
    10981 tgtaaaatct cttttccgtc ttcggcaatt ttctttttct cctcgcgtaa tgaatttttt
    11041 atcttggatt ttgctctcga tgttgtaaca atatttagcc agtcttcgtt aggtttttgt
    11101 tttttggaag taatgatttc tatctggtcg ccattgtgca gcacatgact gatagacacc
    11161 agtttgccgt ttacctttgc cccgatacag gtatcgccca cattggtatg cacgccatag
    11221 gcaaaatcga gtgcagtagc tccggcaggc aatattttca tttcgccctt tggcgtaaac
    11281 gtataaattt ctttatggta aagctgcagt ttaaaatcgt tgataaattc tatcggatta
    11341 ctatcggggt ttttcaaaaa atcacgcact tgcatcagcc agctttcgat ggcttcttct
    11401 gcattattgg taggcttgaa tttattttct ttatacttaa aatgggctgc aaatcctttt
    11461 tctgccacct catccatgcg cttggaacga atctgcactt ctacccactg ccctcccggt
    11521 cccattactg tggtatgcaa actttcataa ccgttggctt ttggatgcga aatccagtcg
    11581 cgcaaacggt tgggtgaagg tgaatattta tcggttacga tagaataagc acgccagcaa
    11641 tcttcttttt ctttctccgg cggcgagtct aaaattacac ggatggcaaa tttatcatac
    11701 acatcttcaa atgaaacgcc cttggttttt atcttgttgt agatggaaga aatggattta
    11761 ggtctgccga aaacatgcgc atgtaatcct gccttgttta actcttcctg caaaggacga
    11821 atgaaatcgt tgatatattt tgtgcggtct tttttagtgg cttccaaacc tttggcaatc
    11881 tctttatact gctccgtttc ggtatatttc atcgccaaat cctcaagctc gcctttgata
    11941 ttatataagc ccaaacgatg tgctaatggc gcatagaggt aaatcgtttc ggatgaaatt
    12001 ttcagttgat tttttcggct ctgcccatct aacgtacgca tattatgaag ccggtctgca
    12061 atttttatca gaatcacacg tatatcatca ctgagtgtca ataagatttt acggtagttt
    12121 tccgcttgaa tagattcagt ctgatgcgaa attttactga ttttagttaa gccgtcaata
    12181 atttttgaca cggtagcacc aaattctttt ttgatatcgt ccagcgtgag gtcggtatct
    12241 tcaacagtgt catgcagcaa tgcaccaatc acggaagtag tacccaagcc gatatcgccg
    12301 gacactattt ttgctacagc gatcgggtgg tggatataag gttcaccgct tttgcggcgc
    12361 atgtggcggt gtgcatcagc agccatctcg aaggcaagac gcaagcgttt gttatcgcct
    12421 ttctgcattc ttggttgtgc caaacgcaac aactggcgat agtctttcag tatcgctgct
    12481 ttttcctgtt ccgcattaat ttccatagag atatacaagt atacttcttt tagtggatat
    12541 tttcagcaaa agtttatgga atggcttttt tctacaataa tttattggca ctttctagca
    12601 accatgcata ttcaataata tctttgtttg aaattaattc atcaataaac ttttgtattt
    12661 gaattttatc cctgttcagt tgtgtaagtt tcaggacata ctttatgaaa tttttgtatc
    12721 gagttttgta ataatcatta atttcttcat ttgtatatac atacttttta aatgcattga
    12781 cactgtttgc caataaatca aaatacgttt catccgattt ctctaattca aaataggttt
    12841 taatcagcag caccctggag cttaaacgat aaaatacatc tttatatttt gtagtactaa
    12901 taagttgcaa aactagttcg tattcttttt tataatagag taaatgagct ttattatagc
    12961 tgtaaacatc ttcttgttca tgttcattta aattgtattt atattcctct acaaactgct
    13021 ctacaaaatc atatttttgt aaatacaatc ctatagtagt tacattttta taaatagcag
    13081 aagtgatggt tttatcttct tctaagatta gattattgtc tatcagaaat ttgtataatt
    13141 caaagagccg taattgtgca ccgtctatat tttggtgtac ttgttttatt gctatagaaa
    13201 ttaatataat cacacaatct ttaagttcat atccttctat ttgctctgaa tgggaaatta
    13261 gtaagtcaaa acatctatga aaatcctcta cagataaatt attttgaagt aatttattaa
    13321 tttgatgatg taaatcaaat aagatattat tatttacatc aatttccaca tattcataat
    13381 tattgattag aattgcattc ttatacttcg tctcagccaa tttattaaac gaagataacg
    13441 agtctaagaa ttcaatatag tttgcttgtc taaaatttga ttgcgaattt tgttcattta
    13501 ttatttcatt taattgtact ttatcattca atttttgcaa gctttctttc gtcttgagca
    13561 aatattgtgc tgtttgcttt atttcacttt caaagtatct gttcattttt tttctttgat
    13621 aaaattttat caaagccatt ttttgtaaaa actgcctttg ttcaacatat tcagctataa
    13681 taaacatttc tactgattta ttacaatcac tgataagttt gttgatttta tgttcattat
    13741 ataattctcc cggaaacaac tttgcaaata ttattttctt ttccaaatca ttttcatcca
    13801 ttacttttga ttgcaagcat ataattatat agttgattaa ttgccattct gcatctttgt
    13861 atatgctcga atccgccaga aatttcaaaa ggcgtttttt ttcttcatga ttcaaagaaa
    13921 aaataatttc atatagcttt gtattaaaca tttttgtaca tttataaaaa aattcacaat
    13981 tatgaaaaga atcttattat catttttagt attcacccta tttcttgctt gcaagaaaaa
    14041 caaaacagaa actatcccat ttaaatttac aaaaatttcg ggaaacagca ctttatcttc
    14101 taagggtata ttaggtttag aatgggtaga taacgagaat accagctgga atatcttatt
    14161 acgcaattta aacaccgggg tgttgactac cagaactacg acaaaccaca atctaacaga
    14221 tagtatagta ttaactgata attatcgtat tactgttaca ggaaataaaa taccagtaga
    14281 tacgactaat ccatgtaata ccggctcggt aaatgctaga attggaaaca tgcctgactt
    14341 attcactacc ttcaatccat gtccgccatt ttcaattact aacgtatttg cagaaaaagt
    14401 gaatgcaacg acagcaacta gtactttagc ttggtctgat gctaataata cctcttggaa
    14461 agtaaaaatt actaatctta caactgctgt atcaaatact accagtgttg cagtaactga
    14521 tctgacattg aatttcgcat tagatcagcc tcaattaata gaaataagcg gtgatcagaa
    14581 tggggcaaaa aaatctgtaa atgttcaagt aaataatgat gcaacttcta ttgtttctaa
    14641 tttttattaa cttctaaaag attgtttttt tcaagaatgt aaaattccgt aaccaatttt
    14701 acacactgaa tatcaatcag ttaaatatta taacgagtcc tttagggctc gtttttttgt
    14761 tttatctgct tcaatactcc gcttatttgt aattttttac ctcttcttat tcctgcaatt
    14821 ttacatcatc aaacagcgcg aattgataaa gatgatgaag cagagacatc tttccaaata
    14881 aaaaaatgcc gccgaaaccg aaaagcgaaa cagagtagga aataaaaaat gaaaaaaatt
    14941 caatcacaat taaatctaaa aaaatgaaaa catccatcaa aaaacaaatc gccatcgctc
    15001 ttgtagccat cttcagcatc gtgtcgttga acagttgcaa aaaaaatgaa accactatta
    15061 atcgtccaac tgcaacagaa tttaatgcgt tgcaagatgc agcactcaat agtctgaaag
    15121 aaacgcatac atttttagca gaagccggta ctacgtttac ttc
//
LOCUS       DAOQII010000030        14958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1904_length_14958_cov_6.074817, whole genome shotgun sequence.
ACCESSION   DAOQII010000030 DAOQII010000000
VERSION     DAOQII010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14958
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1904_length_14958_cov_6.074817"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLF48_02650"
     CDS             complement(<1..964)
                     /locus_tag="PLF48_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNF48252.1"
                     /translation="MNLQQTYHRALNFEFLSIEEGIFLFENAPTAELMQIAHQLRLIQ
                     KPDHIVTWQIDRNLNTTNVCIANCKFCNFYRKPGHEDAYITDIGTYKHKIEETIRWGG
                     DQLLLQGGHHPDLGLKFYTDLFKQLKQLYPTIKLHALGPPEIAHICKLDGVTHTQALT
                     ELKNAGLDSLPGAGAEILNDRVRRLLAKGKCSGQEWLDIMRAAHQLDITTSATMMFGH
                     VESVYERMEHLVWLREVQSEKPTHAKGFLAFIAWPYQDEGTLLRRMRNIRNSVTPDEY
                     LRMVAISRMMLPNIKNIQASWLTVGKQTAQLCLYGGANDFGSIML"
     gene            complement(972..1997)
                     /locus_tag="PLF48_02655"
     CDS             complement(972..1997)
                     /locus_tag="PLF48_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HNF48253.1"
                     /translation="MNILEMNLENKIVTEHQTPPVNLSQADISLFEHEFTYQHTRPVL
                     TPYRSVYFNSLGYIYNKYRLIFFKDCNKISDRPAYVNFIKLKILLTGRIDAPAISIHS
                     IWCEGYFHWLTESLNRLMYIIQSGYHLNDYILLLPKKYLGYSYINESLSLFGFNLENI
                     MPIEEYGNYLCKELLICPPLAIPGRYDYAVSNTASEYMLMKAGLNNLIADRRVYISRQ
                     NSPKRKVTNELELIKLLREYDFEIVETENMTFIQQMSLFNSTRWLISIHGAGLTNMMF
                     MQKHTNILEFREENDKWNNCYFSLASAHNLNYYYLKCKKEGEQKDHVGNLAVNIQELK
                     QLMDLYI"
     gene            complement(1966..2913)
                     /locus_tag="PLF48_02660"
     CDS             complement(1966..2913)
                     /locus_tag="PLF48_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF48254.1"
                     /translation="MSERKRVLITGAAGFLGSHLCDRFMAEGYHVIGMDNLITGNIKN
                     IEHLFPSKDFEYYHHDVSRFVHVPGKLDYILHFASPASPIDYLKMPIQTLKVGSLGTH
                     NLLGLAKEKKARMLIASTSEVYGDPTVHPQPEEYWGNVNPIGPRGVYDEAKRFQEAIT
                     MAYRNFHGLETRIVRIFNTYGPRMRLDDGRALPAFMSQALNNEDITVFGDGSQTRSFC
                     YVSDLVDGIYRLLLSDYHLPVNIGNPQEISLLDFAEEIIKLTGTKSKIIYQPLPKDDP
                     KQRQPDITKAKEILGWQPRIDRSEGLKITLEYFRDELGK"
     gene            complement(2934..4262)
                     /locus_tag="PLF48_02665"
     CDS             complement(2934..4262)
                     /locus_tag="PLF48_02665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNF48255.1"
                     /translation="MNIAVVGTGYVGLVSGTCLAETGNHVTCVDVDTAKVEKLKAGIM
                     TIYEPGLDVVFNRNIKEKRLHFTTNLEDAISNAKLIFLALPTPPGEDGSADLRYVLGV
                     AEQLGKLIKEYKVIINKSTVPVGTAEKTREAIAKNATVEFDVVSNPEFLREGVAVDDF
                     LKPDRVVVGTRSERARKLMEELYRPFVRQGNPIIFMDERSSEMTKYAANSYLATRITF
                     MNELANLCELTGADVETVRQGMGTDNRIGKRFLFPGIGYGGSCFPKDVQALAKTAEEH
                     HYDFKILKAVMHVNDRQKHVLVEKIFNKFGNDLSGKKIAIWGLAFKPNTDDIREAPAL
                     YIIEDLLNAGAEIVAYDPEAKENVKRYFSDSPKKNKLNFTEDYYAPLNGADFLCILTE
                     WTEFRTPDFSKIKSLLKEAVIFDGRNLYDLDDMKSEGFYYNSIGRKTIQA"
     gene            complement(4296..5705)
                     /locus_tag="PLF48_02670"
     CDS             complement(4296..5705)
                     /locus_tag="PLF48_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48256.1"
                     /translation="MYLTILSIFTSLEGHLSGQKLYLNDIIFGLIFGIFSYLVVKKIA
                     DTYYKNTIFYKYLMTFYKVKILSTIFFYLIFAFYYKGGDTFAYLHNTLQLRKILSNDF
                     SAAMAIIFDPHSFKALYNMKGMMIEDGLYMLDDSTHVIITFGFFVGFLCFNSYFVFCL
                     SCSMVAMYGCWKLFQTFVELYPHLHKELAISCLFIPSVCFWGSGFLKDPICIGALGVF
                     TNSLYYIAFKNKKVFINIILSTISVYLISKIKVYIVLAYAPAALIWIISRNKDRIKSS
                     FLRAIFAPLLLILGLLMGLVSLQLMATFADKYSFENMMRTAQDTQNWLVYSSQTAGST
                     SFYTLGNIEYTPLGLLKVFPAAVNVSLFRPYIWEAKKPILMISSVEGIITMGFTIFLL
                     YKSGIWNTIKQILLNPDVLFCMVFSIIFAFAVGFTSFNFGALARYKIPFMPFYYIALF
                     ILADTSKKSKPASLPPKKR"
     gene            5836..7365
                     /locus_tag="PLF48_02675"
     CDS             5836..7365
                     /locus_tag="PLF48_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF48257.1"
                     /translation="MSKIKADSILYITFFFNSLVLGWIINTVNIQQIRTNRQVNNITS
                     GFSLVHNQTINSIDNYWYINHIKNYLKTGEFTYDTTSRLNAVRRTPLYPMFYGLHYVV
                     FGEAGSFKAIKITQMLLYACSAVLLYIATFRFTGNKKVALLTYLIYATFLPFITYLSY
                     TITEAVSPALNCIFLYFLSRTKQNNTIKNWLCSGIMFSILVLTRPNSIFVIFSVLFLL
                     IYYSKQSLKKILYNGLAFVLGSILFFLPWMIHNYKKTNGDIILLEKYYGDQMDYGMPN
                     MHLKYWIASWMNPGDYSSEIISNACIHTIIYQPETDKYKFTDSLLQKTPSAAWKGNKR
                     QDVRDAYLSLYDYYYSKINHVHKDTLAQLEAKSIENFNQLKTEFIKQAPFYHYVLVPL
                     QYAKSLIFQSNSSTLAFLQNYESNPLLYFLKLYLYIFNIICYISLPLLLILYKKYKDI
                     FWITLLYVSSTFFIIMYVNKNFEVRYTYTYFPMLFMCVAIVITEISNSLFVRLNRLKQ
                     H"
     gene            complement(7362..8024)
                     /locus_tag="PLF48_02680"
     CDS             complement(7362..8024)
                     /locus_tag="PLF48_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48258.1"
                     /translation="MFAKDCIDTSIPYLKPTDTAGFALDMMSENKTLILPVVKNHVLL
                     AIADENNLLEVDDNILIEHFISSEESPIHFAEGLHIFDVLKKCAQWNNYFIPVLNNHK
                     HYLGMTSPKHILTAYTQYSALNNPGGIIELQMDAKDYSLTEISKIVESNNAMILHSMI
                     STQKESSQIIVAIKINKSDLQDIQATFERFGYTVVSVLHHSEYEMQLKERLDSLMRFL
                     EV"
     gene            complement(8080..10533)
                     /locus_tag="PLF48_02685"
     CDS             complement(8080..10533)
                     /locus_tag="PLF48_02685"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNF48259.1"
                     /translation="MLLISASALFAQTFIEGKVVDKATKSPISYANIYIKGSTIGTTT
                     DDTGYFQLKLTTYYDSLTAAFLGYKEKSVPILKKSHQIIVFELGEDENMLAEAVIYAS
                     SESFEDYLIRNILSHKEANDKKKLDNYSYKSYNKIELDIKNFGDKLTERKILKPFSFI
                     FNNIDSTSEDEPFLPMMLSETLSEFYYTNKLHKKREIITASKVSGVEDASFSEFLGAT
                     YQDLDIYDNQYNIIQKQFISPIANSCKTFYKYKVIDTLVLDNVVHYKMMFEPKTKGDY
                     TFYGSMIVSENNFAIKDMQLRMAPHVNINFIKRIELNQKFDFVDNKFWMITDDNLLIE
                     FRPLEKKPAIVARRNTIYRDFRINQPYNDSVINKLKEEVTLDENTIKKDAAFWDTSRY
                     INLSKNEAAVYHMIDTLKTIKAYKAYSDIIQLLLTGYYDVGPVELGPILNVVSNNRLE
                     GWRFRMGIQTSESLVKWMQAGAYLAYAIKDKRFKYGAFADFLIDKFPRQTIGASYIDD
                     VGATSRTTDLLSEDNFLNVVIRRKKAPYMVMYMKTAKLNYTIEYKQGMSLSFSMQNRR
                     LDQGTIPFNYVHNYTKQGVDYSDTINHINQTEFVLRARWGFGEKFINKNSLFRSSLNA
                     GNIPVLQFEYVAGIPSVFKSQFTYHKLTIAFSDIIKVNTVGRFEMRFQAGKVFGKVPF
                     LLSEVHLGNDTYIYTNTAFNLMNPYEFYSDQYLQWSFIHHFDGFFLNRIPGIRKLKLR
                     EVIQTRGVWGSVSQKNTELNATNSFIHPIGKVPYIGVGFGLENILKFLRWDVMWRVTH
                     RDNSNAPKWLMTVGFNFNF"
     gene            10651..11430
                     /locus_tag="PLF48_02690"
     CDS             10651..11430
                     /locus_tag="PLF48_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNF48260.1"
                     /translation="MENTLELISYNLNGVRSAVSKGLANWIKLTNYDIYLFQETKAHE
                     YDIPKELFEELGYHVYMHSAQKKGYSGVAILTKLKPDFVNNGIGIKKYDDEGRLIRMD
                     IGDITIINSYFPSGSSGEERQNVKMNYLDDFFTYIHELKQSRPKIIISGDYNICHQAI
                     DIHDPVGNKNNSGFLPEERAWMTKYFDSGFIDTFRHFNAEVKHEYSWWSFRANARNNN
                     KGWRIDYNVVTNELKTQLLDAKIFQEVKHSDHCPVYLKIKR"
     gene            11477..12211
                     /locus_tag="PLF48_02695"
     CDS             11477..12211
                     /locus_tag="PLF48_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HNF48261.1"
                     /translation="MSYRFILVCFILCAGFNPVKSQTADTIFSADTVVVVSKIISIDS
                     TVKRKFVFYPPHADTICKKGCRLRNEETKFGRKLGRGLVFTFGYNSMITGLLLVMPED
                     VSKWNKANYKNQLKSAYTGPPVIDHDKWYINYLGHPYQGTIYYNALRSQGANVWQSAL
                     FCAGSVWLWEYTIEAGFEQPSIQDLIVTPGAGILLGELFHYSTIRMSRNGYTWYEKMV
                     VSILNPTFAINNGFKFAGNNKIKHKF"
     gene            12377..13582
                     /locus_tag="PLF48_02700"
     CDS             12377..13582
                     /locus_tag="PLF48_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48262.1"
                     /translation="MLKWLRLIFIFLCVISIFYIICLNTKLAQQTVWKNGVNTEVQKQ
                     LHFIGQQLEQHGAEKMQQYYPEGYFFSNVLYGLTWCDFLQKTQDKEMRKMGINEINKA
                     TSALSSPKGKSVFPKQLSPQYGNFYAGWTNYLLAKKLAIQNSYADSLRYEQQCDSIVN
                     AYEQNNQTFLASYVGLSWPCDNIVAVASLAMHDKIYPAKYTSFLSEYIQKLKSNLDKD
                     GLIPHKADYKTGLITEQAKGSSQSLMLIFLREVDTNFANEQFAKYKIHFLDKRLGLYG
                     VREHKIGISGKGDVDSGPVIWDIGGSASVVGAKTFALFNETSIAETLRNSLEAFGMAI
                     QNQNDKKYLFGQWLVADCFIAWSNVSESENEQHYDKSSKLTFGLYSIAVILLLLALIK
                     ILLPCKQQL"
     gene            13647..14735
                     /locus_tag="PLF48_02705"
     CDS             13647..14735
                     /locus_tag="PLF48_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48263.1"
                     /translation="MTKLGKMLKIDVEKLRNSDASHLFWMDLTILIIIIINLFYLLFG
                     YLFQFHFFTQAIHTILPSFYSWYAKYIYPNVLNYDIIFVAIYVCELLFRWARSIYRGK
                     YAKWWFYPFVHWYDVIMCIPINQAFSIFSFLRLFGLVFRLQRLGAFDVTKTFVFKKTK
                     FVSDIMVEEVSDAVIVKMLTMAQEEVQKGSPLFEKIVTNVIKPKEEIIVKYLTNRVGE
                     AINIAYTEYRGELKQYTYKIVGESINENKELKTLNYIPGLGNFIKETLDRAVADITFN
                     TIDKLMKDIIDPENTRGIKEVTHALIETFMDHDHKEGEQIHKTVVAMVNESLEEVKQV
                     VNQKEWKIKQLREEKSKLEEKIKESKEN"
BASE COUNT         4772 a         2696 c         2640 g         4850 t
ORIGIN      
        1 cgagcatgat ggagccaaaa tcattagccc ctccatataa acagagttgt gctgtttgtt
       61 tgcccaccgt taaccacgac gcctggatgt tttttatatt gggcaacatc atacgactaa
      121 ttgccaccat gcgcaggtat tcatcgggtg ttacactgtt gcgaatattg cgcatacgtc
      181 tcagcagggt gccttcatct tgatagggcc aggcaatgaa tgccaagaaa cctttggcat
      241 gtgttggttt ttcgctttgc acctcacgaa gccaaaccag atgttccata cgctcgtaaa
      301 cagattctac atgcccaaac atcatggtag cagaagtggt aatgtctaac tgatgggctg
      361 cacgcataat atctaaccat tcttgcccgc tacattttcc ttttgccagc aatctgcgta
      421 ctctgtcgtt taaaatttca gcacctgcac cgggcaaaga atccaatccg gcattcttta
      481 attcggtcaa agcttgagta tgtgttaccc cgtctaattt gcagatatgt gcaatctccg
      541 gaggacctaa tgcatgtaat tttatcgtgg gataaagttg cttgagttgt ttaaataagt
      601 cggtgtaaaa ttttaaaccc aaatcaggat gatggccgcc ctgaagcaag agctggtcgc
      661 caccccagcg gatggtttct tctattttgt gtttatatgt gccgatatcc gtaatatagg
      721 catcttcgtg tccgggtttg cggtagaaat tacaaaattt gcaattggca atgcagacat
      781 tagtggtatt gaggtttctg tctatttgcc aggtgactat atgatcaggt ttttgtatga
      841 gcctaagttg atgtgcaatt tgcatcaact cagctgtagg ggcattttca aacaaaaata
      901 taccctcctc tatagagaga aattcaaaat taagtgcacg atgataggtt tgttgtaagt
      961 tcattataaa attaaatata caaatccatc aattgcttta gttcttgaat atttacagcc
     1021 aaattgccta catgatcctt ttgttcccct tcttttttac attttaaata gtaataattt
     1081 aaattatgag ctgaagcgag tgaaaaatag cagttgttcc atttgtcatt ttcttctcta
     1141 aactctaata tgttcgtatg cttttgcata aacatcatat tagtaaggcc ggcaccatgt
     1201 atgcttatca gccatcttgt agaattaaat aatgacattt gttggataaa ggtcatattt
     1261 tctgtttcta ctatctcaaa atcatattcc ctaagtaatt ttatgagctc aagttcgttc
     1321 gttacttttc tttttggaga gttttgcctg gatatatata cgcgcctatc cgcaattaaa
     1381 ttatttaaac cagctttcat gagcatatat tcactggcag tattcgaaac agcatagtca
     1441 tatcttccgg gaatagccaa agggggacaa attagcagtt ctttgcataa ataattacca
     1501 tattcttcaa taggcataat gttttctaga ttaaacccaa acagactgag ggattcatta
     1561 atatatgaat agccaaggta ttttttcggg agtaataaga tgtaatcatt caaatgatat
     1621 ccggattgga taatatacat tagtctgttc agagactcag tgagccaatg aaaatacccc
     1681 tcacaccaaa ttgaatgaat agatattgca ggagcatcta ttctgcctgt aagtaaaatt
     1741 ttaagtttaa taaaattaac ataggcagga cggtctgaaa ttttattaca atctttaaaa
     1801 aagattagtc tgtatttatt gtatatataa ccaagtgagt taaaataaac agagcgatac
     1861 ggtgttaata ctggtctggt gtgctgataa gtgaattcat gttcaaaaag agagatatct
     1921 gcttgtgaaa gatttaccgg aggggtttgg tgctctgtaa ctattttatt ttccaagttc
     1981 atctctaaaa tattcaagtg taatctttaa tccttcactt ctgtctattc ttggttgcca
     2041 gcctaaaatc tcttttgctt ttgtaatatc cggttggcgt tgcttaggat catcttttgg
     2101 caatggctga taaatgattt tactcttagt tccggtaagt tttattattt cttctgcaaa
     2161 atccaataag ctaatttctt gcggatttcc aatatttaca ggtaagtgat aatcacttaa
     2221 caaaagacga taaataccat ctaccaaatc agatacataa caaaaggatc gtgtttgaga
     2281 accatctcca aaaacagtga tgtcttcatt atttagtgcc tgactcataa atgcgggtaa
     2341 tgctcttcca tcatcgaggc gcatacgcgg accataggta ttaaaaatgc ggactattcg
     2401 agtttccaat ccgtggaaat tacggtaagc catagttata gcttcctgaa atcgtttcgc
     2461 ttcatcatat actccacgcg gtccaattgg attgacattg ccccagtatt cttcaggttg
     2521 tgggtgaacg gttggatcgc catatacttc agaggtggag gcaattagca ttcttgcttt
     2581 cttttctttt gccaaaccta ataagttatg tgtacctaat gaacctactt ttaaagtttg
     2641 tataggcatt ttcaaatagt ctataggtga tgcaggtgaa gcaaaatgca aaatgtagtc
     2701 taatttaccc ggaacatgta caaagcgact gacatcgtga tgataatatt caaaatcttt
     2761 agagggaaac aagtgctcaa tgtttttaat atttcctgta atcagattat ccattccgat
     2821 tacatgataa ccttcagcca taaaccgatc acataaatgc gatcccaaaa aacctgcggc
     2881 tcctgtaatt aatactctct ttctttctga catgataagt tgtttattta tatttatgct
     2941 tgaattgttt ttcgacctat actgttataa taaaagcctt cacttttcat atcatccaga
     3001 tcatataaat ttctgccgtc aaaaattacg gcttctttta gtaatgattt gattttagaa
     3061 aaatcaggtg tcctgaattc tgtccactct gttaaaatac aaagaaagtc tgctccgttt
     3121 aatggtgcat aatagtcttc tgtaaagttt agtttatttt tcttagggga atcggagaag
     3181 tatcttttta cgttttcttt agcttcaggg tcataagcaa cgatttcagc cccggcattt
     3241 aataaatctt cgataatata caatgcagga gcttctctga tatcatctgt attgggttta
     3301 aatgccaatc cccaaatggc aatcttttta ccggataaat cattcccgaa tttgttgaaa
     3361 attttttcaa ccaaaacatg cttttgtctg tcgtttacgt gcatcacggc ttttaagatt
     3421 ttgaaatcgt agtgatgttc ttcagctgtt ttagccagtg cctgcacatc tttgggaaaa
     3481 catgagccac catatcctat acccggaaac aagaatcgct ttccaattct gttatcagta
     3541 cccatgcctt gccttactgt ttctacgtct gctccggtta attcgcacaa attcgccaat
     3601 tcgttcataa aagtaatgcg tgtggccaga taagagttgg ctgcatattt tgtcatttca
     3661 gaactgcgtt catccataaa aataattgga ttcccttgtc ggacaaaagg tctgtatagt
     3721 tcttccatta attttctggc tcgttctgac ctggtaccta ctactacacg atcaggttta
     3781 agaaaatcat caacagccac gccttctctt aaaaattcag gattagaaac tacatcaaat
     3841 tcaacggttg cattttttgc aatagcttca cgcgtttttt ctgccgtgcc tactggaacc
     3901 gtactcttgt taataataac tttgtattcc ttaataagtt ttccgagttg ctcggcaact
     3961 cccagtacat atctcaaatc agcagagcca tcctctcccg gtggggttgg tagggcaaga
     4021 aagattaatt tggcatttga tatagcatcc tccagatttg tagtgaaatg aagtcttttt
     4081 tctttaatgt tgcgattaaa aacaacatct aaaccgggtt catagatagt cataattccc
     4141 gccttaagtt tttcaacctt agcggtgtcc acatctacac aagtaacatg gtttccggtt
     4201 tcggccaagc aagtacctga aaccaaccct acataacccg tacctacaac tgcgatattc
     4261 atttgtttaa ttttgcaagg taaaaatagt aaaaatcatc gtttttttgg aggcaatgaa
     4321 gcaggctttg attttttact tgtatcagcc agaataaaca gcgcgatata gtaaaaaggc
     4381 ataaaaggta ttttatatct ggccaaggct ccaaaattaa aactcgtaaa tcctacagca
     4441 aatgcaaaga tgatagaaaa taccatacaa aataaaacat ccggatttaa taagatttgt
     4501 ttaatagtat tccagattcc agacttgtat aataagaata tagtaaatcc catagtaata
     4561 ataccttcta ctgacgaaat cataagaatg ggctttttgg cttcccatat atatggcctg
     4621 aatagagata catttacggc agcaggaaaa acttttaaaa gccctaaggg agtatattca
     4681 atatttccta atgtatagaa agaggtactt ccggccgttt gagaagaata aaccagccag
     4741 ttttgagtgt cttgtgctgt tctcatcata ttttcaaatg aatatttatc agcaaatgtg
     4801 gccataagtt ggagtgaaac aagccccatg agaagaccta aaattaatag tagtggagcg
     4861 aaaatagccc ttaagaaact actcttaata cgatctttgt ttcgcgagat tatccatatt
     4921 aatgcagcag gagcatatgc aagaactata taaaccttaa ttttagatat taagtatact
     4981 gatattgttg ataaaatgat gtttataaag acttttttat tcttgaatgc aatgtaatac
     5041 agtgaattag taaacacacc caaggctcct atacaaatag gatcttttaa gaaacccgaa
     5101 ccccagaaac aaacagatgg aataaataag caagagatgg ctaactcttt atgtaagtga
     5161 ggataaagtt ctacaaatgt ttgaaacaac ttccagcaac catacatggc taccattgag
     5221 caagaaaggc aaaagacgaa atatgagttg aaacaaagaa aaccaacaaa gaaaccaaaa
     5281 gtaataatta catgtgtact gtcatcaagc atatataaac catcttctat catcatccct
     5341 ttcatattat aaagtgcttt aaaggaatga ggatcaaaaa taattgccat tgcagcacta
     5401 aaatcattag acaaaatttt tcttagctgt aaggtattat gcaaataagc aaaagtatct
     5461 ccacctttat aatagaatgc aaaaattaaa tagaaaaaaa tagtactaag aatttttacc
     5521 ttataaaaag tcattaagta tttataaaag atagtgttct tgtaataagt atctgctatt
     5581 tttttaacta ctaaataaga aaagataccg aagattaatc cgaagatgat atcatttaaa
     5641 taaagcttct gcccacttaa atgaccttcc aaactcgtaa atatggataa tattgttaaa
     5701 tacacaaatg gtaattaatc tattcctgct tgtaatctat tgcgataaag atacacacaa
     5761 taattcttct gcatccgact actttgtaac aaattttata ccttgttgcg aaagataaaa
     5821 ttaccaatca tcaacatgag caaaattaaa gcagatagca tattatatat tacattcttc
     5881 tttaattcat tagtgttggg ttggataatt aataccgtta atatacagca aataagaact
     5941 aatcgtcaag ttaataatat tacttccgga tttagcctcg ttcacaatca aacaattaat
     6001 agcatagaca attactggta catcaatcac atcaaaaatt atctgaaaac aggagaattt
     6061 acgtatgaca ctacctctcg tttgaatgct gtaagaagaa cgcctctgta tcctatgttt
     6121 tatggattgc attatgttgt gtttggcgaa gcgggaagtt ttaaggcaat aaaaattacc
     6181 cagatgctct tatatgcatg ctctgcagta ttattgtata ttgccacctt ccgttttact
     6241 ggcaataaaa aggtagcctt gcttacctat ttgatttatg ccacattttt gccgtttatt
     6301 acttatttat cttatacgat aacagaagct gtaagccctg cattaaattg catttttctt
     6361 tattttttat cccgtaccaa acaaaataat accataaaaa actggctttg ttccggcatt
     6421 atgttttcaa ttttggtttt aactcgaccg aattcaattt ttgtcatatt ttctgtactc
     6481 tttttgctga tctattattc aaaacaatcg ttaaagaaga tactttataa tggccttgcg
     6541 tttgttttgg gcagtattct gtttttcttg ccctggatga tacataatta caaaaaaaca
     6601 aacggtgata tcatcttatt ggaaaaatac tatggagatc aaatggacta cggcatgcct
     6661 aatatgcacc tgaaatactg gatagcctcc tggatgaatc cgggtgatta ttcttcagaa
     6721 attatcagca atgcctgtat ccatacgata atctatcaac cagaaactga taaatacaaa
     6781 tttacagact ccttactaca aaaaactcca tcggcggcat ggaaaggaaa caaacgacaa
     6841 gacgtaagag atgcttacct ttcgctttat gattactatt acagcaaaat aaatcatgtt
     6901 cataaagaca ccctagcaca gttggaagct aaaagtatcg agaattttaa tcaacttaag
     6961 acagaattca ttaaacaagc acctttttat cattatgttt tagtaccatt acaatatgcc
     7021 aaaagcctta tttttcaaag caattccagc actttggcat ttcttcaaaa ttatgagagc
     7081 aatcccttac tttacttttt gaaattatat ttatatatat ttaatattat ctgctatata
     7141 tcactgccgt tacttttaat tctttataag aaatacaaag acatattttg gatcacctta
     7201 ctttatgtat cttcaacttt ctttatcatt atgtatgtca ataaaaattt tgaagtgcgt
     7261 tatacgtaca cctattttcc tatgttgttt atgtgtgtgg ctatagtaat tacggaaata
     7321 agcaatagtt tgtttgtaag attaaacaga ctaaagcaac actaaacttc gagaaaacgc
     7381 ataagactgt cgagtctttc ttttaattgc atttcatatt cggaatgatg caatacggaa
     7441 accacggtat atccgaatct ttcaaaagta gcctgaatgt cttgcaaatc gcttttgttg
     7501 atttttatcg caactataat ttgtgagctt tctttttgtg tagaaatcat gctgtgtaaa
     7561 atcatagcat tgtttgactc tacaatctta gatatttctg tgagcgaata atctttggca
     7621 tccatttgca attctataat accaccggga ttattaagtg cagaatattg tgtgtatgct
     7681 gtcaaaatat gcttgggcga agtcataccc aaataatgtt tatgattatt cagcacggga
     7741 atgaaataat tattccattg tgcacatttt ttgagcacat caaaaatatg taagccttct
     7801 gcaaaatgaa ttggagattc ttctgaactg ataaaatgct caatcaaaat attatcatcc
     7861 acctccagca gattattttc atctgctata gccaacagca catggttttt aactacaggc
     7921 aaaatcaaag ttttgttctc actcatcatg tctaatgcaa aacctgcagt atctgttggt
     7981 ttcagatatg gtatagaagt atcaatgcaa tctttagcga acacgtcttg ttgtttgttt
     8041 ttgtaaaatt ataaaatatg cgttgttatt tccgaataat taaaagttaa aattaaaccc
     8101 gacagtcatc agccatttgg gtgcgttgct attatctcgg tgagtaaccc tccacattac
     8161 atcccatcgc aaaaatttca gaatattttc caatccaaaa cccaccccaa tataaggcac
     8221 tttgcctatg ggatgtatga acgagttggt ggcatttaat tccgtgtttt tttgtgatac
     8281 actgccccaa acaccacgag tctgtatcac ttccctcaac tttaatttac gtatacccgg
     8341 tatcctgttt aagaagaaac catcaaagtg gtgtataaag ctccattgaa ggtattggtc
     8401 gctgtaaaat tcatacggat tcatcagatt gaaagccgta ttggtgtaga tatatgtatc
     8461 gtttcccaaa tgtacttcag acaataagaa tggcactttc ccaaaaactt tgccggcttg
     8521 gaatctcatt tcaaaccgtc cgacagtatt cacttttata atatccgaaa aagctatggt
     8581 tagtttgtgg taggtaaact gacttttgaa aaccgacggt ataccggcaa catactcaaa
     8641 ttgcagaacc ggaatatttc ctgcattaag cgaacttctg aatagagaat ttttattgat
     8701 aaacttttca ccaaaacccc agcgagctct aaggacaaat tctgtttgat taatatgatt
     8761 aatcgtatcg gaataatcaa caccttgttt ggtatagttg tgtacatagt taaatggaat
     8821 agtaccctga tcgagtctgc gattttgcat actgaaagac aaactcatgc cttgtttata
     8881 ttctatcgta tagttgagtt ttgccgtttt catatacatc accatatacg gtgctttttt
     8941 tcgcctgata accacattca gaaaattatc ttcagaaagc aaatcagttg tgcggctggt
     9001 ggctcccaca tcatcaatat agcttgctcc tattgtttgt ctggggaatt tatctatcag
     9061 gaaatctgca aatgccccgt atttaaaacg cttatctttt atggcataag ccagataagc
     9121 gcctgcctgc atccatttta ccaggctctc tgaagtttgg atgcccatgc gaaaccgcca
     9181 gccttccaac ctgttattac tgactacatt aagtatcggt cccagttcta ccggacccac
     9241 atcataatag ccggtgagca gtagctgaat gatgtcgctg taagctttat aagcttttat
     9301 tgttttcagt gtgtcaatca tgtggtaaac agcggcttca tttttgctca ggtttatata
     9361 gcgcgaagta tcccaaaatg ctgcatcttt ttttattgtg ttctcatcaa gagtcacctc
     9421 ttcttttagc ttattgataa ccgagtcatt atagggttga ttgattctga agtctctgta
     9481 aattgtattt cttcgggcaa caattgccgg ctttttctcc aacggacgaa attctatcag
     9541 cagattatca tcggttatca tccagaattt attgtcgaca aaatcaaatt tctggttgag
     9601 ttctatgcgt ttaataaaat tgatattgac atgtggtgcc atgcgaagct gcatatcttt
     9661 gatggcaaaa ttattttctg aaacaatcat gctgccgtaa aaagtataat cgcctttggt
     9721 tttgggttca aacatcatct tataatgtac tacattgtct aataccaagg tgtcaattac
     9781 tttatacttg taaaatgtct tgcagctgtt ggctatcggg ctgataaatt gtttctgaat
     9841 gatattgtat tggttgtcgt agatatccaa atcctgatac gtagcaccta aaaattcaga
     9901 aaagctggca tcttcaacac cagatacttt cgatgccgtt ataatctctc tctttttgtg
     9961 aagtttgttg gtataataaa actcagacaa tgtttcagac aacatcatag gcaaaaaagg
    10021 ctcatcttct gaggtgctgt caatattgtt aaaaataaaa ctaaacggct tcagtatctt
    10081 gcgttcagta agtttatcgc caaaattctt tatatccagt tcaattttgt tatagctttt
    10141 atagctatag ttgtccagct tttttttgtc gtttgcctct ttgtgcgaca ggatatttct
    10201 tatcagataa tcttcaaaac tttcagatga ggcatatatt accgcctctg caagcatatt
    10261 ttcatcttct cccagctcaa aaacaataat ctgatgtgac ttttttaaga tgggcacact
    10321 tttttcttta taccctaaaa acgcagccgt cagtgaatcg taataggtag taagctttag
    10381 ctgaaaatag ccggtatcgt ctgttgtagt gcctatagta gagcctttta tgtaaatatt
    10441 ggcgtagctg atgggtgatt tagttgcttt atctactacc ttgccttcta taaaagtctg
    10501 tgcaaataat gcagatgcac ttattaacag cacaaaaagg aatgttattt gcggcaactt
    10561 cattcggtag tgaaaatact tataatacgt cggattttta tatccaaagg ttgttaatca
    10621 aaagcaatac caaatttata tttttacaac atggaaaata cattagaact tatttcttat
    10681 aatctgaatg gtgtccgctc tgctgtttct aaaggcttgg caaattggat aaaactaacc
    10741 aactacgata tttacttatt tcaggaaacc aaagcacatg agtatgatat acccaaagag
    10801 ctttttgaag aactgggcta ccatgtttat atgcattctg cacaaaagaa agggtatagt
    10861 ggcgtggcta ttcttacaaa actaaaaccc gattttgtaa acaatggcat cggcattaaa
    10921 aaatatgacg acgaaggtcg gctcatccgg atggatattg gagatattac gattatcaat
    10981 tcctatttcc cttcgggcag cagtggagag gaacgacaga acgtaaaaat gaattatctg
    11041 gatgattttt ttacttacat ccacgagctc aagcaaagca gaccgaaaat aattatcagc
    11101 ggcgattata atatctgcca tcaggcaata gatatacacg acccggttgg caataaaaat
    11161 aactccggat ttttgcctga agaaagagcc tggatgacta aatatttcga ttccggcttt
    11221 attgatacat ttcgacattt taatgcagaa gtaaaacacg aatattcgtg gtggagcttc
    11281 agggctaatg cccgcaacaa taataaaggc tggcgaattg actacaatgt tgttactaac
    11341 gagttaaaaa cccagctact cgatgcaaaa atatttcagg aggtcaaaca ctccgaccat
    11401 tgtccggttt atttgaaaat aaaacgatag tagctaaaat ggacaaatgg tatgtttttt
    11461 ggtgaagggt atagtaatgt catacagatt cattttagtc tgttttattt tgtgtgccgg
    11521 ttttaatccc gtcaaatcac aaacagcaga cactattttt tctgcagata ccgtagtggt
    11581 ggtctctaaa attatcagta ttgacagcac agtaaaacga aagtttgttt tttatcctcc
    11641 acatgcagat actatttgca aaaaaggatg tcggttaaga aatgaggaaa caaaatttgg
    11701 cagaaaatta ggtcgtggac tcgttttcac gttcggatat aattcaatga ttaccggatt
    11761 gcttttagtc atgcctgaag atgtaagtaa gtggaataaa gccaattata aaaaccagct
    11821 aaagagtgca tataccggac caccggtcat cgatcatgat aaatggtata tcaattatct
    11881 tggacatccc tatcagggca caatctatta caacgccctc cgctcgcaag gtgctaatgt
    11941 atggcaatct gcacttttct gtgcaggaag tgtttggctc tgggagtata ccatagaagc
    12001 aggcttcgag cagcccagta tacaagattt aattgttaca cccggcgccg gtattttatt
    12061 gggtgaatta ttccattaca gcaccatacg catgagccga aatggatata cctggtacga
    12121 aaagatggta gtcagtattc tcaatcccac tttcgccatc aataatgggt ttaaatttgc
    12181 aggaaataac aagataaaac acaagtttta gcattcattc tttgtataat gctgcttagt
    12241 caagtgtgat tttttttgta aatagttgaa tgtcattatt taatcttagc ttttaattat
    12301 aattatttaa gaaaagcaat ccttctaata accattctta gtaattgtaa aggacaggat
    12361 tttctaattt taattcatgc taaaatggct tagactaatc ttcattttct tgtgcgtaat
    12421 aagtattttt tatatcattt gcctgaatac taaactggct caacagacag tatggaaaaa
    12481 tggtgtaaat accgaagtgc aaaaacagct gcattttatt gggcagcagc ttgagcaaca
    12541 tggtgcagaa aaaatgcagc aatattatcc cgagggttat tttttctcga atgtcttata
    12601 tggattaaca tggtgtgatt ttctgcagaa aactcaagat aaggagatgc ggaaaatggg
    12661 cataaatgag ataaacaaag ccacatctgc actgtcttct ccaaaaggaa agtcagtatt
    12721 tccaaagcaa ctaagccctc aatatggtaa tttttatgca gggtggacaa actatctttt
    12781 ggctaaaaaa ctggcaatac aaaacagtta tgccgatagt cttcgttatg aacaacagtg
    12841 tgacagcata gtgaatgctt atgaacagaa caatcagaca tttttagctt cttatgtcgg
    12901 tttgagctgg ccttgtgata atattgttgc ggtggcatcc cttgccatgc acgataaaat
    12961 ttatcctgca aaatatactt ccttcttgtc tgaatatata caaaaactga aatctaattt
    13021 agataaagac ggcttaattc cacataaagc agattataaa acaggactga taacagaaca
    13081 agctaagggc tcttcacaga gcttaatgct catttttctt agagaggttg atactaattt
    13141 tgccaatgag caatttgcaa aatacaagat tcattttctt gataaaagat taggtcttta
    13201 tggtgtaaga gaacataaaa ttggaataag cggaaaaggt gatgtagact ccggtcctgt
    13261 aatttgggat ataggaggtt ctgcctcagt agtaggtgca aaaacatttg cattgttcaa
    13321 tgaaacttcc attgcggaaa cattgcgcaa cagcttagag gcttttggta tggcaattca
    13381 aaaccaaaat gataaaaaat acttattcgg gcaatggtta gtggctgatt gttttattgc
    13441 ctggtcaaat gtaagcgaat cagagaatga acagcattat gataaaagca gtaaactgac
    13501 gtttggctta tatagtattg cggtgatatt gttgcttttg gctttaataa aaattctttt
    13561 accctgcaaa cagcaattgt agtttatcag cccaattcat catggcattt aatttatgct
    13621 tatcttcact aaaaaagtaa gataatatga ctaagctcgg gaaaatgctg aaaattgatg
    13681 tcgaaaaact gcgcaattca gatgcctcgc atttgttttg gatggattta accatcctga
    13741 ttatcatcat catcaatctt ttttatttac tttttggcta tttatttcag tttcacttct
    13801 ttacccaggc gatacatacc attttgccat ccttttacag ttggtatgca aaatatattt
    13861 atccaaacgt cctaaattat gacataatct ttgttgccat atatgtttgc gaattgctat
    13921 ttagatgggc aagaagtatt taccgaggaa aatatgcgaa atggtggttt tatcctttcg
    13981 tacattggta tgatgtaata atgtgcatcc ccattaatca ggcattcagc atatttagtt
    14041 ttcttcgttt attcgggttg gttttccgcc tgcaacgatt aggtgcattt gatgtaacca
    14101 agacatttgt ctttaaaaaa acgaaatttg tatcagatat aatggtggaa gaggtgagtg
    14161 atgctgttat cgttaaaatg ctgaccatgg cacaggaaga agtgcaaaaa ggttcacctc
    14221 tttttgaaaa aattgtaaca aatgtaatca aacctaaaga agagataatt gtaaaatacc
    14281 tgactaacag agtgggcgaa gccattaata ttgcctatac ggaataccgt ggagaactga
    14341 agcaatacac ttataaaatt gtgggggagt ctataaatga gaataaagaa ctaaaaaccc
    14401 tgaactatat tccaggattg ggcaatttta ttaaagaaac actggatcgt gcagtagccg
    14461 atatcacctt taataccata gacaagctca tgaaggacat catagatcct gaaaatacga
    14521 gaggaattaa agaagttacg cacgccttaa ttgaaacctt catggatcac gaccataagg
    14581 aaggcgaaca aatacacaaa acggtggtcg ccatggtaaa tgaatcgctg gaggaggtga
    14641 aacaagttgt taatcagaaa gaatggaaaa tcaagcagct acgagaagaa aagagcaaac
    14701 tggaagaaaa aattaaagaa tcaaaagaaa attaaccaac aaaaatggac tttggcacga
    14761 gaattgttac tttattgtaa acgaaaatcg tttaatttca tttggttggt ttaagatagt
    14821 cccggcggtt ggttaccccg ggatttttta ttgaacaacc tgagttcggg aaaagcgttt
    14881 caattaaaaa tagttgattt ttgatataga accacctcct tttaattcca cctccttgtt
    14941 aagttggagg aaaaaatg
//
LOCUS       DAOQII010000031        14877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_1918_length_14877_cov_5.385306, whole genome shotgun sequence.
ACCESSION   DAOQII010000031 DAOQII010000000
VERSION     DAOQII010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_14877_cov_5.385306"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLF48_02710"
     CDS             <1..328
                     /locus_tag="PLF48_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48264.1"
                     /translation="RICVGILKNVDESAHTAVLEDVIVLQASSAPDISQILAQASAKT
                     GTEHAFALAQLLAVPTSGGTIRAITQNEMQNEMFATSSAQNALYFFIKPHTESNKFYC
                     LKRGLS"
     gene            410..2794
                     /locus_tag="PLF48_02715"
     CDS             410..2794
                     /locus_tag="PLF48_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48265.1"
                     /translation="MPDDIFDILNNPDSTLPWPNNLNNNFLWGLTKPKNEYISPFVSS
                     PMFKYRKDTNICVSENAPLLSNALGDNKYMYNYFIYNYNDIVDLLETYNSPTGYIGDY
                     EAYFYVERNNPNESNFFGTFVLCNLGKEYKKHIERTIAGISEVNFYDYYHNNKGTLNS
                     FNEVAKFYQLVFNQANNDDIDFDEVSFALGHALNTNIKSDEGIFGIDEKAAGWIKGLA
                     EKIENHKFKEEDYNFEANQQDKDDSEFKEAIEEILDIHKKIIKKIAKIILPSNVQEFL
                     KKDAVKFVEEVVLKALPPKLQLLLQNLIKKAQEVKSIIQQGYELAKDLIAETKKIFTA
                     FLMGVANGFMSLIQMLLEIVAWCVDKVLGFQLGLDTDKAMNGTHYIQARKVMERMEDM
                     YDLISENLGSFIESIKNLFSSLDKKELSRLLDTIKTKLGDTIDKLSKYDIAYFCGNIV
                     FEIIVGVVLALLTGGASVIAQISTRAGKVAQLLKIILQETVSAVTVGASDLFNIFKSL
                     WLKFKEVAGRGFKNFVDYIINILTKGIDDIKVDVTPDDLASVDNFKNVDEDLIKAKLD
                     EITSTRKPTPKERISKKQAEQKARIEKKKKSSFIKRTKEDYLKERKKLIAISKEDGGW
                     SRIFRLYQKSIFKEMTKKELVDFYELMINKFPELERKKINFAAFEIDIYKGGKKIETV
                     VEFAHSGPKKISTDFIDTIPDTEFNKLELIDDNLLTRKNDTEVKFIYNFFENHIHKGD
                     YFVIRSKNIYMACGSCQREFVTLNQLLGNKVKISVIGRTEILNSYDFENFINNK"
     gene            2802..3281
                     /locus_tag="PLF48_02720"
     CDS             2802..3281
                     /locus_tag="PLF48_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNF48266.1"
                     /translation="MELIYMNKLKNMPQIYNRNNRGLTVLQIEELEKKYNNGNPFPKA
                     YREFLVIAGEYPNLGAHYDAPGYDWMQEEARNELEECNKTIDRPFFVIDQLDGCTQFS
                     FIYLDETDEDPKVYHCHAHPSYWKDHGFIVERKFNPFSKLIDYCIDLAIRDYKAYYE"
     gene            3638..3970
                     /locus_tag="PLF48_02725"
     CDS             3638..3970
                     /locus_tag="PLF48_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48267.1"
                     /translation="MAGEYNGGLIMFPGHNDLAMLSKESQLEKLKKRVQEENLKIDRP
                     FWVFTEADGFQQFLFFYLDEDPIDPEVYGVTYRKESEYYVYDLGKKFSEYEDAVIDRS
                     ILSDQRGY"
     gene            4259..4735
                     /locus_tag="PLF48_02730"
     CDS             4259..4735
                     /locus_tag="PLF48_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNF48268.1"
                     /translation="MTIQHLKKLEQNKIIRNSKLEGVSESAIKQVEIRLNIKFPETYK
                     EYLYLAGEYNGGLIMFPGHNDLATLSSDLQQKYLKEQLSYANLKIDRPFWVFTEMDSF
                     EQFLFFYLDEDPIDLEVYGVTYRKESDYYVYDLGNKFSEYVDAVIDRSILYAQRGY"
     gene            complement(5111..6592)
                     /gene="proS"
                     /locus_tag="PLF48_02735"
     CDS             complement(5111..6592)
                     /gene="proS"
                     /locus_tag="PLF48_02735"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNF48269.1"
                     /translation="MSKEITSRAENYSEWYNDLVLKSGLADYSAVRGCMVIKPYGFTL
                     WENMRDVLDSKFKQTGHVNAYFPLFVPKSLFEAEEKNAEGFAKECAVVTHYRLKSSPE
                     DKSKLIVDPEAKLEEELVVRPTSEAIIWNTYKKWIQSYRDLPILVNQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATRMEAEAETVQMINVYADFVENYMALPVIKGVKSESERF
                     AGAEDTYTIEALMQDGKALQSGTSHFLGQNFAKAFDVKYVNKENVEEYVWATSWGVST
                     RLIGALIMAHSDDDGLILPPKIAPYQVVLIPFLSKNAEETEKMIAKANEFKAELSAAG
                     IRVLVDTDETKRPGFKFAEYELKGIPLRVVIGPRDLANGVVEIARRDTKEKSQLPLEN
                     FVENIKSLLEDIQQNMFNKAKAFRDENITKVDDFETFQKLLDEKPGFILAHWDGSAET
                     EDKIKELTKATIRCIPLDNPQEDGKCILTGNPSKERVLFARAY"
     gene            6781..7884
                     /locus_tag="PLF48_02740"
     CDS             6781..7884
                     /locus_tag="PLF48_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48270.1"
                     /translation="MKNINRVLSFVMALFAITSVFAQDDVYDAPRVKKVKVQQTSDAP
                     SYSGSTYEEKTAPYYGEESSDYTNTNQRNSQYDYDEDNYAMTYSERIQRFHNPNVRFN
                     LSWNNCINNWYDPYYNYNSYSASSWGWYNSFTPSWWMPSTQIIIINPGYSSWWSNSWN
                     NPYYSWNSWNYGCGNNYWGNSWNSWNSWNSWGWNSPVCGNNWGWGNNYYNNYNHYGYS
                     GYNNNWYDNGNIHNPKNTTYTPRHTTYNNTNNHNFNNNNNTGQNTNGYTQPNQPSNTN
                     NSYNKPNKQWDTPKNQNTGKPDVYQNSPRNNNNNTNNNNWNNSPSKTNNSNSGNNGWN
                     NAPSNNNSNSGWNSSPSNNNSNSSGVKTGSRPK"
     gene            7904..9412
                     /locus_tag="PLF48_02745"
     CDS             7904..9412
                     /locus_tag="PLF48_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48271.1"
                     /translation="MKKNVVFLGLLMSIVCLLHAQTDIDALRYSLTNFQGTARNISVG
                     NTMSSVGADISNVHTNPAGLAKYSATEISVTPGVSFYKSNSEFLNNKISQNKIKFQLG
                     SAGIVFGKRSADEENKKKWSKPTFAINISRLANYNEKYFMSGYNADNSILNAYWEKLN
                     DRSVITDSIAAVTQYPFDASLAFALGLITIDTLGNTYTATNNGNMTQSIQINRTGGMD
                     EAAIGIASEYKDKLMLGLSLGIPFVSYSENMILTEKDDSNYAGHLTSFSVENRLKTTG
                     TGFNLKAGVIYSPISNLKIAFSVQTPGLIFMKDRFYSHMEADYDSLSVILTADSPDGE
                     FKYRYRQPWKMGLGVSYVHKYGFVATEYELTDVSNSKFNFKSSTAADIAYENFVNSNI
                     KNKYGLTHTLKVGTEFKIQKFRIRAGFQYRSTPFDKAAQPTAFKTYALTYSGGLGYRG
                     NHFFVDATFVQTQTKEMYIPYTIDSSFWLSSAPEAKLSISKPAVFVTLGYKF"
     gene            complement(9432..10127)
                     /gene="radC"
                     /locus_tag="PLF48_02750"
     CDS             complement(9432..10127)
                     /gene="radC"
                     /locus_tag="PLF48_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNF48272.1"
                     /translation="MYSQEKFSIKQWAEDDRPREKLVLKGKHTLTDAELLAIILGSGS
                     VELSALDLAKRVLSSVNHDLYYLGKLTLTDLKSFKGIGEAKAVSILAALELGKRRKLA
                     EKSESSKIISSNDVFDVMKQDIGDLKHEEFWILCLRQNNTLIAKHKISMGGVSRTIVD
                     PKLVFNIALRDLASSIVICHNHPSGNLKPSEVDIQLTKNIHTNARLLEIALLDHVIVT
                     ENGYYSFRDNNQL"
     gene            complement(10191..10448)
                     /gene="rpsT"
                     /locus_tag="PLF48_02755"
     CDS             complement(10191..10448)
                     /gene="rpsT"
                     /locus_tag="PLF48_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNF48273.1"
                     /translation="MAHHKSAQKRIRQTAKRRLENRYYGKTARNAVRKFLATKDSAEA
                     QKLMPTVISMVDKLAKRNQIHKNKAGNIKSEIMIHMNHLKK"
     gene            10808..12466
                     /locus_tag="PLF48_02760"
     CDS             10808..12466
                     /locus_tag="PLF48_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48274.1"
                     /translation="MFARLKHINEKNVLRFDRYTILSLLLLCAIGIKVSWNLSKYMDV
                     MFWDEALYMERGLAMFAIIPKTWGPAYSLWYKLVSFFVHENVSLYYFNFKLTSLLVSI
                     LLYLVMLSMGTQRILAFIFAMFFMSSYLNLPVWPHISHFVIIVILSGILIAKFHQSLL
                     EKLLIWSAAFLMATYARPEMFFPFLLVYGVAVIIFIIRIKQLHKREIIYAGLLTITVF
                     LVSSKIGTPLNSGDTQRGIKVFLQHYAWRYSEDHHLKNVFWLEFMDIDKTQFGNKVYS
                     THFSELLSAYPDKIGHHLSLNFKEYLLQTGKLLVNFFVPIFTEKPHWLALMIALFMLI
                     IYFSLNRKTSSFSHRIVSLLRMNLWSLLMLLLMLLSSVVVCIYAYPREHYLLLQVPFL
                     LVSISLLISAVSIERQDTYSKYIVLGVVWFFVTPESEDFRYFSLPDVNGKLPNQKAIS
                     LIKKEFTQKDSIRIFDVEGYVRTMLPANFTDYNSAFFADTTILPSNHILQNKIDIVYV
                     SSTFRLFNRITRDSIMTDMLKHPETYGYLSRKSAENINLLIRKR"
     gene            12463..13788
                     /locus_tag="PLF48_02765"
     CDS             12463..13788
                     /locus_tag="PLF48_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48275.1"
                     /translation="MKNFAYQTLYTKDEPLFEVKVFLFFLYSLIFSSNVLLQFKSMNA
                     SIGAIVVMILPFVFIITGVYYYNKILVRFELNIIDVLVLITFFFPIYNGIMSYLTLGV
                     PIKKMLMVLSARMYIVLTSFTYYLVRTNRLTVKEYVYGNILLCWFSMLLYMYISLTQN
                     PALYKDSLEEGLVGYNPSKGGYIFRFSSSFLYFGIIYYYLSYILNGSIFGLICWFILI
                     AYQLFIDKGRSELIAELIPLMLYSVIILKWHQLIKKMLLIFLLAAIMFGIAYWIDPRI
                     VKFTADMYMKFFEFFIGKKTGEGSADMRWIEFGHIYDYFIKHPGQIFFGIGTPKKEDM
                     YRYVGEVILSDTGIVGCVLANGIVGTILLHLLLLHPIYVFLKVKHYKHDILFNTGILG
                     CLTVVIQSMFTGMFYYNPNALMIFIIIVEYYRVKEKFYWKSHPKLNIST"
     gene            13785..>14877
                     /locus_tag="PLF48_02770"
     CDS             13785..>14877
                     /locus_tag="PLF48_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48276.1"
                     /translation="MKVIFLNLSVLILTTFSSPSCMQNAGKNTADKNETTYLADKIID
                     TTPISISISTTTPYTSFSAVNKHIFGLNIGFAFVKELDKDSGFVQLLRAMHPDNLRFP
                     GGTVGNYYHPDAPAYGFKFLEIYPALYSLYNQQSKRNENILQNYLRLTRDVNANGIFC
                     ANVLTGSPEEVLQVIDVLKKNKVNLLGVELGNEFSGEEYRKKFPDAKTYINAIQATAN
                     AIKLKYPDLKLAVIGSIPVAKTDQSQRAKFMRAWDNELKQVNFYDAVVVHEYTACPPC
                     TKESKTVEESFTKNMAIIGPIKLNKIELYKQYYQATYTSAKKIWMTEWNIADPNFEGT
                     FLQGIFVYEMFLNLLDMNVRDNIPYEITNA"
BASE COUNT         4987 a         2505 c         2683 g         4702 t
ORIGIN      
        1 gcgcatatgt gtaggcatac taaaaaatgt ggacgaaagc gcacatacgg cagtactgga
       61 agatgtaatt gtgttgcagg caagcagtgc acccgatata tcacaaatac tggcgcaggc
      121 atctgctaaa accgggactg aacatgcatt tgcattagca caattgcttg ccgtgcccac
      181 gtcgggcggt acgataagag ccattaccca aaatgagatg caaaacgaaa tgtttgctac
      241 aagcagtgca caaaatgcct tgtatttttt catcaagccc catacagaga gcaataaatt
      301 ttactgcctc aaacgcggct taagctaata cgcttagtat aaccacctgt ctcgtttcgt
      361 atagagcgga gcgaggcagg cattttttaa ctttttaaaa ttaacaatta tgccagacga
      421 tatctttgac atattaaata atccggactc aactctacca tggccaaata atttaaataa
      481 taatttcttg tggggcttga ctaaaccaaa aaatgaatac ataagcccat ttgttagctc
      541 tcccatgttt aaatatagga aagacactaa tatctgtgta agtgaaaatg ccccactgtt
      601 atctaatgca ctaggggaca acaagtatat gtataattac tttatatata attataatga
      661 tatagttgac ttgttagaaa cgtataattc acccacaggc tatattggtg attatgaagc
      721 atatttttac gttgagagaa ataaccccaa tgaaagtaac ttttttggaa cgtttgtatt
      781 atgcaattta ggtaaagagt ataaaaagca tattgagaga acaatagccg gaataagcga
      841 agtaaatttt tatgattact atcataataa taaagggact ttaaattctt ttaatgaagt
      901 agctaaattt tatcagttag tatttaatca ggcaaacaat gatgatatag attttgatga
      961 agtttctttc gcattaggac atgcattaaa tacaaatata aaatctgacg aaggaatatt
     1021 cggtattgat gaaaaagctg cagggtggat taaagggtta gcagaaaaaa tagaaaacca
     1081 caagtttaaa gaagaagact ataattttga ggcaaatcaa caagataaag atgattcaga
     1141 atttaaagaa gctattgaag aaattttgga tatccataaa aagataataa aaaaaatagc
     1201 gaagataata ttacctagta atgttcaaga attcctaaaa aaagatgcag ttaaatttgt
     1261 cgaagaagtc gttctaaaag cattgccacc aaaactacaa ttattacttc aaaatctgat
     1321 aaaaaaagca caagaagtaa agtcaataat acagcaaggg tatgagcttg ctaaagatct
     1381 aattgccgaa acaaaaaaaa tatttacagc atttttaatg ggcgtagcaa atggttttat
     1441 gtcacttatc caaatgctac ttgagatagt agcatggtgt gttgataaag tattgggatt
     1501 tcaattaggt ttagatacag ataaagctat gaatggcact cattacattc aagctcgtaa
     1561 agtaatggaa agaatggaag atatgtatga cctgatttct gagaatttag gttcatttat
     1621 agaaagcata aaaaatcttt tcagttcctt agataaaaaa gaactttctc gattattgga
     1681 tactatcaaa acaaaattag gcgatacgat tgataaatta tctaagtatg atatcgccta
     1741 tttttgtggc aatatagtat ttgaaattat agtaggcgtc gtacttgcct tacttactgg
     1801 aggtgcaagt gtaatagcac aaatttccac aagagcagga aaagtcgcac agttacttaa
     1861 aataatattg caagaaactg tatctgcggt aaccgtagga gcttctgatc tttttaatat
     1921 ttttaaatct ctatggttaa aatttaaaga agtagcaggt cgaggattta aaaattttgt
     1981 tgattatata attaatattc ttacaaaagg tattgatgat attaaagtag atgttacacc
     2041 tgacgattta gcatctgttg ataattttaa aaatgtagat gaggatctta ttaaagcaaa
     2101 attagatgag attacttcga cacgtaaacc aaccccaaaa gaacgtatta gtaagaaaca
     2161 agccgaacaa aaagcacgta ttgaaaagaa aaaaaagagt tcatttataa aacgtactaa
     2221 agaagattat ctaaaagaaa gaaaaaaatt aattgcaata tctaaagaag atggcgggtg
     2281 gtcaagaatt tttcgtttat accaaaaatc tattttcaaa gaaatgacaa aaaaagaact
     2341 agtggatttt tatgaattaa tgattaataa atttccagaa cttgaaagaa agaaaattaa
     2401 ctttgctgct tttgaaatag atatttataa aggtggaaaa aaaatagaaa ctgttgtaga
     2461 attcgctcat tcagggccta aaaagataag tactgatttt attgacacaa taccagatac
     2521 agaatttaat aagctggaac ttatagatga taatttatta actaggaaaa atgatacaga
     2581 agttaagttt atttataact tttttgaaaa ccatattcat aaaggcgatt attttgtaat
     2641 tcgttcaaaa aatatttaca tggcatgcgg tagttgtcaa cgcgaatttg taactttaaa
     2701 tcaactttta ggcaataaag taaagatttc agtaataggt agaacagaaa tccttaactc
     2761 ttatgacttt gaaaatttta taaataataa ataatataac tatggaatta atatatatga
     2821 ataaacttaa gaatatgcca cagatatata atagaaataa tagaggctta actgttttac
     2881 aaatcgaaga acttgaaaaa aagtataata atggaaatcc atttccaaaa gcttatagag
     2941 aatttctagt tatagcaggc gaatatccaa atttaggagc acattatgat gcgcctggct
     3001 atgattggat gcaggaagag gcaagaaatg aattagaaga atgtaataaa acaattgatc
     3061 gtccattttt tgtgatagac caattagatg gttgtacgca atttagtttt atatacttgg
     3121 atgaaacgga tgaagatcca aaggtatatc attgtcacgc acatccaagt tattggaaag
     3181 atcatggttt tattgtagaa cgcaagttta atccattctc aaaattaata gattattgta
     3241 ttgatttagc aattagagat tataaggcat attatgagta atagaaaaag aatgccaaat
     3301 atagttggcg tagatactgc agatttagaa agtatatagg agtagataga aactacatgg
     3361 catgtggtag ttgtcaacgc gaatttgtaa ctttaaatca acttttaggc aataaagtaa
     3421 agatttcagt aaaaggtaga acagaaatta aaagaatctt catttaaaaa acttattaaa
     3481 aaacaaataa aaatggaaat acaatatctt aaaaaattac agcaaaactc caaaaattgg
     3541 aactcagaaa ataagaggtg tttcagcgtc agctatcaaa caagtagaaa tacgtttaaa
     3601 tattaagttt tctgaaacgt ataaagaata tctgtatttg gcaggagagt ataacggtgg
     3661 cttaattatg tttcctggcc ataatgattt agccatgcta tcaaaagaga gtcaacttga
     3721 aaaattaaag aagcgtgttc aggaagaaaa cttaaaaata gatcgtccgt tttgggtatt
     3781 taccgaggcg gacggctttc aacaatttct gtttttctat ttagatgaag accctataga
     3841 tcccgaggtg tatggcgtaa cctaccgtaa ggagtcggag tactatgtat atgatttagg
     3901 caaaaaattc agtgaatatg aggatgctgt aatagacagg tctatcttat ctgaccaaag
     3961 agggtattaa aaaattttat gaaataaaca gtgcagtgcg ccctttggcg cgctttttta
     4021 ttaaaaaaag aatatagatt tacgggtatt atatgcaaaa tatggtgagc agaacgtgca
     4081 gattatagaa caaaacatta aaacattatt cgaaccatgc ctgtcatgca aaaagcaaat
     4141 cgtaatacgg caacagttgt acaatattca gtatctttca atagaagcaa caagatttga
     4201 caaaacaaga tatgtagtag agaacaaaga attcttaaaa gtaataaaat aattaatcat
     4261 gacaatacaa catctaaaaa aactcgaaca aaacaaaata ataagaaact ccaagcttga
     4321 aggcgtttca gaatcagcta tcaaacaagt agagatacgt ttaaatatta agtttcctga
     4381 aacgtataaa gaatatctgt atttggcagg agagtacaac ggtggcttaa ttatgtttcc
     4441 tggtcataat gatttagcta cgttgtcaag tgaccttcag caaaaatact taaaagaaca
     4501 gctaagttat gcaaacttaa aaattgatcg tccgttttgg gtatttacgg aaatggattc
     4561 tttcgagcag tttttgtttt tttatttaga tgaagaccct atagatctgg aagtatacgg
     4621 cgttacctac cgtaaggagt cggattatta tgtatatgat ttaggcaaca aatttagtga
     4681 atatgtggat gctgtaatag acaggtctat cttatacgcc caaagaggtt attaaaaaaa
     4741 attatgaaat aaacagtgca gtgcgccctt tggcgcgctt ttttattaaa aaaaggatat
     4801 agatttacag gtattatatg caaaataccc ccaactcacg caagtatgca gcgtagcctg
     4861 tctgcttaca ggcagtcaat tagtgttgca tacttgcctg ccaatgtgca tacttgtgcg
     4921 tttgtattaa ttctatatct tagcatataa aaaccatgag cagaaaatat aaattcaaag
     4981 acaatgaaga aatcccaacc acgaaacatt gcgtgagaca tcaggtcctt gacttgacga
     5041 agttaaagga ttaacatttt taatgtcaga tggaaacacc tgacttcacc ggaaagtaac
     5101 tttcaaaaaa ttaatacgct ctggcaaaca acactctttc cttactcgga tttccggtta
     5161 aaatacattt cccgtcttct tgcgggttgt ccaatggaat acaacgtatc gtagcttttg
     5221 tcagttcttt tattttatct tcagtttctg cactaccatc ccagtgcgct aaaataaagc
     5281 ccggtttttc atccagtaat ttctgaaaag tttcaaagtc atccacttta gtaatatttt
     5341 catcccggaa agctttggct ttgttaaaca tattttgctg aatgtcttcc aataaggatt
     5401 tgatattttc tacaaaattt tccaacggca actgcgattt ttccttggtg tctctgcggg
     5461 caatttcaac cacaccattg gctaaatcgc gtgggccaat aaccactcgc aatggaattc
     5521 ctttcaattc atattccgcg aatttaaatc cggggcgttt ggtttcgtca gtatccacca
     5581 gcacgcgtat gcctgctgcc gaaagttcag ctttgaactc gttggctttg gcaatcattt
     5641 tttcagtttc ttctgcattt ttagacagaa atggtatcaa tactacttga tacggagcaa
     5701 ttttaggcgg caaaatcaat ccgtcatcat cagagtgagc cattatcaat gccccgatta
     5761 agcgtgtgga aacgccccat gaagtagccc acacatattc ttctacattt tctttgttta
     5821 catattttac atcgaatgct ttggcaaaat tttgacctaa aaaatgagaa gtgcccgatt
     5881 gcaatgcttt cccgtcttgc atcagcgcct ctatggtata cgtgtcttcc gctcctgcaa
     5941 atcgctcgct ttcagatttt acgcccttaa ttaccggtaa tgccatgtag ttttctacaa
     6001 aatcggcata aacattaatc atttgtacgg tttctgcttc agcttccatt ctggtggcat
     6061 gtgctgtatg cccttcttgc cataaaaatt ctgccgtgcg caaaaataaa cgggtgcgca
     6121 tttcccagcg cactacattc gcccactggt tgacaagaat cggcaaatcg cgataagatt
     6181 gaatccattt tttataggta ttccaaataa ttgcttctga ggtaggtcgt accactaatt
     6241 cttcttccag cttggcttcc gggtccacaa tcagtttgga tttatcttca ggagatgatt
     6301 ttagacgata atgtgtaacg acagcacatt cttttgcaaa gccttctgca tttttttctt
     6361 cagcttcaaa aagacttttg ggaacaaata agggaaaata agcattgaca tgccccgttt
     6421 gcttaaattt gctgtcgagt acatcccgca tattctccca aagggtgaaa ccatatggtt
     6481 tgattaccat acaacctctt acggcagaat aatcagccaa tccgcttttc aatactaaat
     6541 cattatacca ttcggaataa ttttctgctc tgcttgtaat ctcttttgac atgatttgaa
     6601 ttttgtgttt aatgttaaag gctaattgtt taagtgtata agaatagttt gataaaattt
     6661 atcttcccct ctccataaaa attaacataa attggtatgt tttttgaaac taaatgttta
     6721 taattttgtt gtatcaaata taggggatta gaaatttcca aaatattaaa aatgactgtt
     6781 atgaaaaata taaatcgggt tttaagtttt gtgatggcat tatttgccat tacttcagtt
     6841 tttgctcagg atgatgtata tgacgcacct cgggtaaaaa aagtaaaagt tcagcaaact
     6901 tctgatgcac cgtcttattc aggtagcact tatgaagaaa aaactgctcc ttactatggt
     6961 gaagaatctt cagattatac aaatacaaac caacgcaaca gccagtatga ttatgatgaa
     7021 gataactatg ccatgactta ttccgagcgc attcagcgtt tccataatcc gaatgtgcgt
     7081 ttcaatttga gctggaataa ttgtatcaat aactggtacg acccgtatta caattataac
     7141 agttattcag cctcatcttg gggttggtat aattcattta caccaagctg gtggatgccg
     7201 tctactcaaa tcattataat aaatccgggc tactcttctt ggtggagcaa ctcgtggaac
     7261 aatccctact attcttggaa tagctggaat tatggttgcg gaaataacta ttggggtaat
     7321 tcatggaata gctggaactc atggaattca tggggctgga actctccggt atgcggaaat
     7381 aattggggat ggggcaataa ttattacaat aattacaacc attacggata tagcggttat
     7441 aacaacaact ggtatgataa tggcaatatt cataatccta aaaacacaac ttatacacca
     7501 cgccatacca cctataacaa tacaaacaac cacaatttca acaacaataa caacaccggc
     7561 caaaatacaa acggatatac acaacctaat cagccgtcta acacgaacaa ttcttataac
     7621 aaacccaata aacaatggga tacacctaaa aaccagaaca ctggcaagcc ggacgtatat
     7681 caaaattctc caagaaacaa taataataac acgaacaaca ataattggaa taattcacct
     7741 tctaaaacaa ataattctaa tagtggcaat aatggatgga acaacgcacc atctaataat
     7801 aattctaaca gtggctggaa tagcagtcca tccaataata attccaatag cagcggagta
     7861 aaaacaggaa gccgccctaa ataaacttaa tgtccataca accatgaaaa aaaatgtagt
     7921 cttcctcgga ttattgatga gcattgtttg cctattgcat gctcaaacgg atattgacgc
     7981 cttgcgttat tcattaacaa actttcaggg aacagcccgt aatatcagcg taggcaatac
     8041 catgtcaagt gtaggtgcag atatcagcaa cgtacataca aatcctgccg gcttggcaaa
     8101 gtattcagct accgagattt ctgtaacgcc cggtgtgtct ttttataaat caaacagcga
     8161 atttctaaac aacaagattt cacagaataa gataaaattt cagttgggaa gtgcaggaat
     8221 tgtttttgga aagcgtagtg cagatgaaga aaataaaaag aaatggagca aacctacatt
     8281 tgccatcaat atctccaggc tggcaaatta taatgaaaaa tatttcatgt cgggctataa
     8341 tgcggacaac agtattttaa atgcatattg ggaaaaactg aatgaccgat ccgtgattac
     8401 agattccata gcagcggtta cccaatatcc gtttgatgcc agtctggctt ttgccttggg
     8461 cttaataact attgatactt taggcaacac gtatacggct accaataatg gaaatatgac
     8521 acaatctatc cagataaacc gcacgggtgg catggatgaa gcggcgatag gtattgccag
     8581 cgaatacaaa gataaattga tgctgggact ttctttggga ataccttttg tgagttacag
     8641 cgaaaatatg atattaacag aaaaggatga cagtaattat gcaggtcatc ttacttcctt
     8701 ttctgtagaa aacagattaa aaaccaccgg aacaggattt aacctgaaag ccggggtaat
     8761 ttattcgcca atcagtaatc tgaaaattgc cttttcggta caaacacccg gattgatctt
     8821 catgaaagac agattttatt cgcacatgga agctgattat gacagtttga gtgtgattct
     8881 gacagcagat tcgcctgatg gtgaatttaa ataccgctac agacaaccct ggaaaatggg
     8941 cttaggcgtt tcttatgttc ataaatacgg attcgttgca acagaatacg aattgacaga
     9001 tgtttccaac tccaaattca atttcaaatc aagcacagct gctgatattg cctacgaaaa
     9061 ctttgtcaat tcaaacataa aaaataaata cggactgacg cacaccctga aggttggaac
     9121 tgaatttaag atacaaaagt tcagaatcag agcaggtttc caataccgtt ctactccatt
     9181 cgacaaagca gctcaaccca cggcatttaa aacatatgca cttacctata gcggaggttt
     9241 gggctataga ggaaaccact tctttgtaga tgctactttt gtacaaactc aaacaaaaga
     9301 aatgtatatt ccttatacga tagacagcag cttttggcta agctcggctc cggaagcaaa
     9361 actttccata tccaaacctg cagtttttgt aacattgggt tacaaatttt aaagaagata
     9421 aatagctttt ctcagagctg attattgtct ctaaaactat aatacccatt ttctgtcacg
     9481 atgacatgat caagtagtgc aatttccaga agtcgggcat tggtatgtat gttttttgtt
     9541 aactggatat caacttcgct gggtttcaga tttccggatg gatgattatg acaaataacg
     9601 atggatgatg ccaaatctct taatgctata ttgaaaacca gcttagggtc aacaatagtt
     9661 ctgcttaccc cacccatgct tattttatgt ttagcaatga gcgtattatt ttgtcttagg
     9721 caaagtatcc aaaactcctc gtgcttcaaa tcaccaatat cctgtttcat gacatcaaaa
     9781 acatcattac tgctgataat ttttgacgat tcactttttt ctgctaattt ccttcgtttt
     9841 cccagttcaa gtgcagccaa tatggaaaca gccttagctt ctccaatgcc tttaaatgat
     9901 tttaagtcgg ttaaagttaa ttttcccaag taatataagt cgtggttgac ggatgaaagt
     9961 acacgtttag ccaaatcaag cgcactcaat tccacactgc ctgaaccaag tataatggct
    10021 aacagttcag catcggtaag ggtatgcttt ccttttagta ctaatttttc acggggtcgg
    10081 tcgtcttccg cccattgctt tatagagaat ttttcctgag aatacatgca atcaaaaaag
    10141 gtgtaccata tagatacacc ttttgtcaat tttccataaa aaagtaggaa ttattttttc
    10201 aaatgattca tgtgaatcat gatttcagat ttgatgttgc cggctttgtt tttatgtatc
    10261 tggtttcttt tggctaattt atctaccata gaaataacgg ttggcattag tttttgagct
    10321 tcagcgctat ctttagtggc taagaacttg cgaactgcat tacgtgcagt ttttccgtaa
    10381 taacggtttt ccaatctgcg tttagcggtt tgtctgattc ttttttgagc cgatttatga
    10441 tgtgccatat ttttaatttt gagtttgcaa agatagcctt tttttcttaa ttattgcaat
    10501 tgtacgtaaa atttagttta aaaatgctat taacctgagt ttgttataag catttcaatt
    10561 aaaaatagtt gattttttat atagaaccac ctccttttaa ttcctccatc ttgataaagt
    10621 ggaggataaa atgtgaaatc aactatttta tcagcttatt tttcttaacc cgaactcagg
    10681 ttataattat agtggaattt aatggcaaaa ctggattttt tgacatgtga aattatatat
    10741 ggcttttaat tctgttttca tatacccgat ttcttaaaat aagtttagaa agaagtatct
    10801 tagtccggtg tttgccagac taaaacatat caacgaaaaa aatgttctcc gttttgacag
    10861 atatacgatt ttaagtcttc tgttattatg tgctatcggg ataaaagtat cctggaattt
    10921 gtctaaatat atggatgtca tgttttggga tgaagcactt tatatggagc gggggctggc
    10981 tatgtttgcc attatcccaa aaacatgggg acccgcctat tccttatggt ataagctcgt
    11041 atcttttttt gtacatgaaa atgtatctct ctattatttt aattttaaac tgacttcttt
    11101 gttggtttcc atcctgttat acctcgtcat gttgtcgatg ggtacacaac gtatactggc
    11161 atttattttt gccatgttct tcatgtcgtc ctatctcaat ttgcccgtat ggccacatat
    11221 ttcacatttt gttattattg ttatacttag cgggatatta attgccaagt ttcatcaatc
    11281 cctattggaa aaattactca tttggtctgc tgcattttta atggccacat atgcccgtcc
    11341 tgaaatgttc ttcccgtttc tgctggtgta tggagtggct gtgattattt tcatcattcg
    11401 aattaagcaa ttacacaaac gagaaattat ttatgcaggt ttgctgacca taaccgtctt
    11461 tttggtgtcg tctaaaattg gcactccact aaacagcgga gatacccaga gagggataaa
    11521 ggtgtttttg caacattatg cctggagata ttccgaagat catcatctga aaaatgtttt
    11581 ctggctggaa ttcatggata ttgataagac acaatttggg aacaaagtgt actcaaccca
    11641 tttcagcgaa ttacttagtg cttatccgga taagatagga caccatttaa gcttaaactt
    11701 taaggaatat ctgttgcaaa ccggcaaact tttagttaat ttctttgtac ccatttttac
    11761 agaaaagcct cattggctgg ctttgatgat tgctttgttt atgctcatta tctatttttc
    11821 gctcaatcgc aaaacatcat ctttttctca tagaatcgtt tcgctgctga ggatgaattt
    11881 atggagtttg ctgatgttgt tgctgatgtt gttgtcttca gttgtggtat gtatttatgc
    11941 ttacccaagg gagcattatc tcttattaca agtaccattt ttgctggtaa gcattagttt
    12001 actgatttcg gcggtaagta tagaaaggca agatacctat tccaagtata ttgtacttgg
    12061 cgtagtgtgg ttctttgtaa cccctgagag tgaagatttc cgctatttct cattgccgga
    12121 tgtaaatggg aaattaccta atcaaaaagc aataagttta attaagaaag aatttactca
    12181 aaaggacagc atccgtatat ttgatgttga gggctatgtg cgtacgatgt tgcctgctaa
    12241 tttcacagat tataactcag ctttttttgc tgatacaacg atattaccat ccaatcatat
    12301 tctgcaaaat aagattgaca ttgtatatgt cagcagtaca ttcagattat ttaacagaat
    12361 tacccgtgat tcgataatga ctgatatgct gaaacatccg gaaacttatg gatatttgtc
    12421 ccggaaatca gcagagaata taaatttatt gataaggaaa agatgaaaaa ttttgcctac
    12481 caaacactgt atacaaaaga tgaacctttg tttgaggtaa aagtattttt gtttttttta
    12541 tacagtttga ttttcagcag taatgtattg cttcagttta aatctatgaa tgccagtatc
    12601 ggtgctattg tagttatgat actgccattt gtatttatta ttaccggagt atattactat
    12661 aataagattt tagtacgttt tgaattaaat atcatagatg tactagtact gataactttt
    12721 ttcttcccta tttacaatgg aattatgtcc tatttaacac tgggagtgcc tattaaaaag
    12781 atgttgatgg tcttatctgc caggatgtat attgtactta ccagctttac ttattattta
    12841 gtgcggacga acaggcttac tgtaaaagaa tacgtgtacg gcaatatctt gctttgttgg
    12901 tttagtatgc tgttatacat gtatatttca ttaactcaaa acccggcatt atataaagac
    12961 tcattagagg aaggtttagt gggatataat ccttcgaaag gaggctatat tttcaggttt
    13021 tcttcatcgt ttttgtattt tggtataata tactattatt tatcatacat actcaacggc
    13081 agtatttttg ggctaatatg ttggtttatc ttaattgctt accagctctt tatcgataaa
    13141 ggaagatcag agctgattgc agaactaatt cctttgatgc tatatagcgt aattatctta
    13201 aaatggcatc agttgattaa aaagatgttg ttaatattct tgcttgctgc gataatgttt
    13261 ggcattgcct attggataga cccacgtatc gtaaagttta cagcggatat gtacatgaaa
    13321 ttcttcgaat tttttattgg gaaaaaaacc ggtgagggtt ccgcagatat gcgttggata
    13381 gaatttgggc atatctacga ttatttcatt aagcatcccg ggcagatttt cttcggcata
    13441 ggaactccta agaaagaaga catgtacagg tatgttggcg aggtaatatt atctgatacg
    13501 ggaatagtag gttgtgtttt ggcaaatgga atagtgggaa caattttact tcatctatta
    13561 ctgcttcacc caatctatgt atttttaaaa gtaaagcact ataaacacga tatcttattt
    13621 aatacaggta ttttaggctg tctcacagtg gtcatacaaa gcatgtttac agggatgttt
    13681 tactataacc cgaatgcatt aatgatattt atcatcatcg tagaatacta tagggtcaag
    13741 gaaaaatttt actggaaatc acatcctaaa ctaaatatat ctacatgaaa gtcattttct
    13801 taaacctgtc tgttcttatt ttaactacgt ttagctcacc ttcgtgtatg caaaatgccg
    13861 gaaagaatac agctgacaaa aacgagacta catatttagc tgacaaaata attgacacaa
    13921 cacccatctc aatttcaatc tctacaacta caccttatac aagcttttct gctgtcaata
    13981 aacatatttt cggcctaaat ataggcttcg catttgtaaa agaactggat aaagactcgg
    14041 ggtttgtcca gcttttaaga gctatgcatc ctgataatct acgtttcccc ggtggtactg
    14101 tcggtaatta ttaccatccg gatgctccgg cttatggctt taaatttttg gaaatttacc
    14161 cggcattata tagcttatat aatcagcaaa gcaaacgtaa tgaaaacatt ctgcaaaatt
    14221 atctgcggct caccagagac gtaaatgcta atggtatctt ttgtgccaat gttttaaccg
    14281 gctcgccgga ggaagtgtta caagtgattg acgtgcttaa aaaaaacaag gtgaatctac
    14341 tgggcgtaga gcttggaaat gaattttcgg gtgaagaata ccgtaaaaaa tttccggatg
    14401 ctaaaaccta tatcaatgca attcaggcta cggcaaatgc aataaaacta aaatatcctg
    14461 atttgaaatt ggccgttatt ggttccattc cagtagccaa aacagatcaa tcgcaacggg
    14521 ctaagtttat gcgagcatgg gataatgaac tcaaacaagt aaatttttat gacgccgtag
    14581 ttgtacatga atatacagct tgccctcctt gtacgaagga aagtaaaacg gtagaagaat
    14641 cattcacaaa aaatatggct atcatcggac ctataaaatt gaataaaatt gagttgtaca
    14701 aacaatatta tcaggctacc tatacatctg caaaaaaaat ttggatgaca gagtggaata
    14761 tagcagatcc taactttgaa ggtacattct tgcaaggcat atttgtgtat gaaatgtttt
    14821 tgaatctgct ggatatgaat gtcagagata atatacctta tgaaatcacc aatgcac
//
LOCUS       DAOQII010000032        14467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2013_length_14467_cov_5.404385, whole genome shotgun sequence.
ACCESSION   DAOQII010000032 DAOQII010000000
VERSION     DAOQII010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14467
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2013_length_14467_cov_5.404385"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..2753)
                     /locus_tag="PLF48_02775"
     CDS             complement(270..2753)
                     /locus_tag="PLF48_02775"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNF48277.1"
                     /translation="MNFRHFLVFITLLLAFHIGYSQIELRGVVIDAKTKEPLAYVNYA
                     IENNQGGTTDDSGRFFMLVNSKTDSVVFSYLGYKDEILKKRFFKKDSIIVKMHLDSYM
                     LEEFTVKAKKGKIPKDTAAIRIFRNVVKNKTLNKPKSYESYQYEEYTKTLASLYNINP
                     KLAKRKILKPVRFVVSNFDSTSTGTKYLPLILKETIKQFYVNKDPKRNKEVVVASKVS
                     GIEQLRFSELLDVAIDDVEAYDNQTILNGKTFLMPFADGGLTLYNYYVIDSAKGADSF
                     MVYNLAFVPKNKGDMLFNGSAKIHGQDYALMQLNLGIDKRSEINWINDFYLNQTFTQT
                     PDGWFKESEDRATSIAFTKRKKAKSVRIARSMHRKDIVTNMAIADTILKQPDYLELKD
                     YRRRKDTFWVNHRHDTLRKSEQNVYILIDSFKNTRFYKAMSQLGGLFASGYYRTKVID
                     IGSLYQMVSWNDLEGVRLRLVLKNNWRIGKWVNWKVYGAYGINDKKFKYGAELGVNLP
                     DKKFKQPHGVSFAYYDDYQRFTLEGTGLDYDYIYNSFLRRKSIADLIYLKDFRASYNR
                     QWLKNFSTSINFNYKTYETIPGKIEFVKTQPDGITKDTTRSFQIFTPSVNIFYAPGAK
                     FFKTSKQTKFLKGNLPRFNFGYSFSIRNFLGSDFNYHKLTLKVEERLPSPIGHTNITL
                     TGMKLFGNAPYPLLTIHPGNQSFIYDEQRFSNMRETEYIADQQLSLIIEHHFDGFFFN
                     KIPGFRKLGLREVFVTKMAVSSLSPEKVAFSDLPVGMTGLNGFYAEIGFGIENIAKLI
                     RADFSWRLTQRDKPGISKFRWTFSFAPNF"
     gene            complement(2876..4540)
                     /locus_tag="PLF48_02780"
     CDS             complement(2876..4540)
                     /locus_tag="PLF48_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF48278.1"
                     /translation="MMKMKKYGILFSLFLSMGLSGNAQMYQQKMGTFLNLLDNYYVED
                     VNVDSLVEIGIREILKSLDPHSVYMDATETKKANEPLEGNFEGVGVQFQIYQDTIQIV
                     HVIEGGPSKKVGIQDGDKIIFVDTANVAGVGITNDGVFKKLRGKKGTHVTVKIKRYGE
                     NDLLDFEIIRDKIPIYAIEAKYMASPDIGYVKLSRFSATATEETQQAIDSLKKAGAKK
                     IILDLTDNGGGYLNQAHALSDLFLENGKLVVYSSGRSQPRIDLNATEAGDFEKGDLVI
                     MVNQNTASASEIVSGAVQDWDRGLIVGRRTYGKGLVQKGYTLPDMSMVRLTMAHYYTP
                     SGRNIQKSYAKGIEKYHEDIEDRYESGELFDEKKLKLTDSTQYFTADKRVVYGGGGIT
                     PDIFVPMDTLWNSKYYQKLVRTGSFNQFTLVYINDNRVAMQKKYPTADAYIKNFSIDD
                     SLLQKFYAFAKDKKAEPDSAEVWSQSIPTIKSQLKGLIGQDLFNSSVYWQVTNLQNPI
                     YVRALNAFTDGSFQKYKITEEKADKRNSSKKTGILSRKRKDKKKAK"
     gene            complement(4581..5312)
                     /locus_tag="PLF48_02785"
     CDS             complement(4581..5312)
                     /locus_tag="PLF48_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNF48279.1"
                     /translation="MDFTIFSQPGTWLSLLTLTFLEIVLGVDNIIFISIVTQKLPDAD
                     RKRGQNLGLFIAMILRIILLFFLGWILGLDKNILPPNAWNLELSWKGVILIVGGIFLI
                     YKSTTEIHHKITGDDDEFNVTNSEKKSSFVSVLIQIALLNLVFSIDSILTAVGIVKEI
                     PVMIIAVVISMLVMMLFTQPVSKIVTKYPSLQILALSFLIMIGVTLIMEGFHYEVQKG
                     IIYTSVAFSFAVELLNIRYRKKNKA"
     gene            complement(5640..6422)
                     /locus_tag="PLF48_02790"
     CDS             complement(5640..6422)
                     /locus_tag="PLF48_02790"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNF48280.1"
                     /translation="MAKVKRKIDICIISDIHLGTHACHALELNRYLNSIDPGVLIING
                     DWLDIWNFSANYFPKEHIENIFLVLEYAQKGTPVYYLTGNHDDHMRNFSGFNFDILQL
                     RDELFLEIDGKKHWIFHGDKYDLSVGGSARWLAKLGGRYYDKIFQLNRWVNNIRMRYG
                     RSQIFISKYIKDKVKSIVKKNVQDFEDVAIEIGIENGFDYIVCGHIHKPQIREVQTEA
                     GKAVYLNSGDWIENLTALEFSHGKWDLFYYQHDLQYKDIDRS"
     gene            complement(6485..7300)
                     /gene="murI"
                     /locus_tag="PLF48_02795"
     CDS             complement(6485..7300)
                     /gene="murI"
                     /locus_tag="PLF48_02795"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNF48281.1"
                     /translation="MPDAHQSIGLFDSGIGGLTVANAILDELPNENLIYFGDTAHLPY
                     GDKSADTIRYFAKTIGSFLLGKDCKIIIIACNTASAVAYEMLQNMAGEKAYVFSVIEP
                     VVDYILKNKYRKVGVIGTLGTVNSKVYQHKIEQRDASIQVSQLATPLLAPMIESGFAH
                     GEISHLVIEEYLNKPELADIECLVLACTHYPLIKKEIVAYYAKHNRKVDVLATNQIVA
                     KHIKHFLIENGLVNFEPKAHHEFYVSDFTESFEKTTQYFYKEKIHLIHLPLFK"
     gene            complement(7400..7963)
                     /locus_tag="PLF48_02800"
     CDS             complement(7400..7963)
                     /locus_tag="PLF48_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF48282.1"
                     /translation="MKKLTTVVLIMIAAIATSFAQKVNKFGYLNSYELLSLMPEKAKA
                     DTVIDKYAKDLDNLANQMAVEYQTKATNFQQKYDKKLMSEAEAELAAKELQDLEQRIN
                     EFQQASQQKVQKKQDDLYSPIMQKVNDAIKAVAKANGYTYIFDSSAGSLLYADESDDV
                     LPLLKSYLKLPEPKPAPQAPKTGTTPK"
     gene            complement(8003..8527)
                     /locus_tag="PLF48_02805"
     CDS             complement(8003..8527)
                     /locus_tag="PLF48_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF48283.1"
                     /translation="MKKQLLSILVLCCFALLTTNSFAQRFAYVDTKYILEKLPDYKSA
                     QIKLDDQAKAWQAEIDQKNEALKKMYTKYQAEEFLLSEDMKKQREEDIIKAENELKDL
                     QKSRFGVNGDLFKKRQELIQPIQDKVFDAIQKLAENRQYDFVFDKASGGSAILYSNPK
                     LDLSEEILKRLGVN"
     gene            complement(8538..11225)
                     /locus_tag="PLF48_02810"
     CDS             complement(8538..11225)
                     /locus_tag="PLF48_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNF48284.1"
                     /translation="MKQQLYIWILCFGLMGKTGMIKAQDTASAEALLLAQDSLPTSIA
                     AANEPKTYTLAALDISGTNFLDKNIIRSLSGLKPGDAITVPGDEVTKLIKNLWRQGLF
                     GNVKVYASNIVGDSIYLRVDVVERPRLNSITVNGLKKSDKEDVLKRLDYYKGKPLTDA
                     LKLNVKNTVLDKFEEKSYLNAQVNVVENAEKGLINAAALTVNVTKGNKVKINNIEFNG
                     VESASLAKLRKAMKGTKERTKVQLFLHDKSRSKKQKAVDALYTLGNLNHKSFIDFFAE
                     RFRINFKGSKFNEEKYEEDKSNLINYYNSLGFRDARIISDTVIKNDDGNVSVKLNIDE
                     GNRYYFRNIEYKGNSKYTNEQLSKVMNIKKGDIYNAELLQKRLNMDPDGGDISSLYYD
                     DGYLFFNVNAIETSAENDSIDLEVRISEGPQATIKNVIIEGNDKTNDNVIRRELHTFP
                     GDKFSRSDLIRSQREIANLGYFDPQETKVEPIPNPIDGTVDIKYTVKEKSADQVELSA
                     GWGGKQGGLIGTLGLKFNNFSLKNIIHKDAWKPLPTGDGQQLTLRIESNGKRYQNYNF
                     SFTEPWLGGKKPNAFTVAAFRSRIQNLDENKKVTGKQITTGMSVSLGTRLKKPDDYFI
                     FQASGNYYSYILQNYLGYYINNVSIDSGKFNNLNFKLSLQRNSINQPVFPTGGAKVEM
                     SFQFTPPYSLFRKDNNFDGKTLSEKYKLIEYYKFKFNVDWYQQLGKSKLVLRTVAKFG
                     FLGAYNKKIGITPFERFEVGGNGIPSTVSLFGTDIVAHRGYEGPYSSTGGDPIYNKFL
                     LELRYPFSLSPTATIFGLAFFEAANTYHSIKEYNPFKLKKSVGIGVRAVLPMFGLIGI
                     DYGIRFDSGNGSPIKNASGFFDYIGKNGNISFILGFEPE"
     gene            complement(11240..12001)
                     /locus_tag="PLF48_02815"
     CDS             complement(11240..12001)
                     /locus_tag="PLF48_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNF48285.1"
                     /translation="MQTNPESYKNKLDCLALPKHIAIIMDGNGRWAKSQGKHRIFGHK
                     NGVKAVREVTEGCAEIGVNYLTLYAFSTENWSRPQKEVSALMELLSLTISKEIKTLQK
                     NNIRLNAIGNINTLPESNRKALMKAISETQNNTRMTLTLALSYGSRDEIIQAVQKIAQ
                     TYKSGFVNLNEIDENFLAANLFTAGMPDPELMIRTSGECRVSNFLLWQMAYTEFYFTE
                     KFWPDFNREDLFHAIYDFQNRERRFGMTTEQLEEK"
     gene            complement(12002..12847)
                     /locus_tag="PLF48_02820"
     CDS             complement(12002..12847)
                     /locus_tag="PLF48_02820"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF48286.1"
                     /translation="MKKRQQIVYLILAIMLNLPLLAQENEVGAWGGTAVYLGDLNPTY
                     SFKNARWATGVFYRYNFGKGRMSVKAGGNYAFLSADDSRIKGYPYLQARNLDFNSQIA
                     ELAITYEINFFKYSTYDYKDGTSKRWTPYLFTGVSMFYFNPITRYDGKRIQLESVGTE
                     GQKSANIQTKNRGYNYYAFAMPYGGGFKIALTQHLALNVEISGRLTFNDYLDDVSKFY
                     AEPTDVNYFINGENVGPDIADKSAGKIGVFGKQRGTSNDRDRFLFIGAGLTYTFITFK
                     CPKVF"
     gene            complement(13068..14051)
                     /locus_tag="PLF48_02825"
     CDS             complement(13068..14051)
                     /locus_tag="PLF48_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF48287.1"
                     /translation="MYKQAFHLSPLQDKKIVVTGGAGFIGSNIVEYLLTHGAKVRVLD
                     NLATGNLKNLEEFRTNPNFEFLEGDIRNFADCEKACDRMDMVCHQAAIGSVPRSIKDP
                     VTSNEVNVNGFVNMLTAAKNAGTKRFVYASSSSVYGDEPNLPKREERVGNPLSPYAVT
                     KFTNEVYASVFSRCYNMEIIGLRYFNVFGPKQDPDGEYAAVMPLFIKSLLNDEAPYIN
                     GDGEQTRDFTFVHNAVQANIKALTVENQDAYNQAYNVAFGERFTINQMYDAIRHYLKS
                     DIPAIHREPRAGDIRDSLADISNAKTKLGYMPQYSFEQGLPLTIEYFKTIL"
BASE COUNT         4279 a         2826 c         2535 g         4827 t
ORIGIN      
        1 attattctcg tgttttttac tcttttcaga cagatgtaga agttaatgat ataaactatg
       61 ggcatagcgt ttttagttgc aaccttaaaa tccaagaatt atgtacgaat agaattctcc
      121 caagcctttg acaagattga gaatacatta gaaactaact gttactgttt ggaaaaatta
      181 agcaacttat tactattact taagcacata aactgagcgt cgacagatga taacacctgc
      241 cgagggcgaa ggaaatttga agaataatat taaaaattcg gggcgaagct gaatgtccat
      301 ctgaattttg atatacccgg cttatcgcgt tgggtaagcc tccacgaaaa atcggcacgt
      361 atcagtttcg ctatattttc tataccaaat ccaatttcgg cataaaagcc atttagtccg
      421 gtcataccca caggcaaatc agaaaaagct actttttcag gactcaggct tgacactgcc
      481 atcttggtta caaagacttc acgtaatccc aattttcgga accccggaat tttattaaag
      541 aagaaaccat caaagtggtg ttctatgata agcgaaagtt gctgatcggc gatatactcc
      601 gtttctcgca tgttggaaaa tcgctgttca tcgtaaataa acgattgatt gccgggatgt
      661 atggtcaaca agggataagg agcattgcca aacagtttca tgccggtaag tgtgatattc
      721 gtatgcccta tcggcgaagg caatcgttct tctactttta gagtcagctt gtgatagttg
      781 aaatctgacc ccaggaaatt gcggatactg aacgaataac caaaattgaa tctcggcaga
      841 tttcctttca gaaattttgt ctgctttgag gttttaaaga atttagctcc cggtgcgtaa
      901 aatatattga cacttggcgt aaatatctga aagcttcggg ttgtgtcttt ggtgatgcca
      961 tcgggctgtg tttttacgaa ttctattttg cccggaatgg tttcatacgt tttgtaatta
     1021 aaattgattg acgtagagaa atttttgagc cactgtctgt tgtaggaagc cctgaaatct
     1081 ttcagataaa ttaaatcggc aattgatttt cttcgcaaaa aggaattata gatatagtca
     1141 taatccagac cggtaccttc caaagtaaat cgctggtagt cgtcgtaata agcaaaggat
     1201 acaccatgtg gctgcttgaa tttcttatcc ggcaaattta caccgagctc agcaccgtat
     1261 ttaaatttct tgtcgttgat gccatacgca ccatatacct tccagtttac ccatttgcct
     1321 attcgccagt tgtttttcaa tactaagcgc aagcgaacac cttccagatc attccagctc
     1381 accatttgat agaggctgcc tatatcaatt accttagtac gatagtagcc actcgcaaac
     1441 aaaccgccca gttgtgacat tgctttataa aagcgtgtat tcttgaaaga gtcaatcaaa
     1501 atgtacacat tttgttcact cttgcgcaag gtgtcgtgtc tgtggtttac ccaaaaagta
     1561 tcttttcgtc tgcgataatc tttcaactcc agataatcag gttgtttgag aatagtatcg
     1621 gcaatagcca tattggtaac aatatctttg cggtgcatac ttcgggcaat gcgcacactt
     1681 ttggcttttt ttctttttgt aaaagcaatg gaggtagcac ggtcttccga ttctttgaac
     1741 catccatccg gagtttgggt gaaagtctgg ttgagataaa aatcattgat ccagttaatc
     1801 tcggagcgtt tatctatacc cagattcagt tgcatcaaag cataatcctg accatgaatt
     1861 ttagcacttc cattaaagag catatcacct ttgttttttg gaacaaaagc caaattataa
     1921 accataaaac tgtctgctcc ttttgccgaa tctataacgt aataattata cagcgtcaaa
     1981 ccgccatcgg caaatggcat caaaaatgtt ttgccattga gtatggtctg gttgtcgtag
     2041 gcttccacat cgtcaatggc cacatcgagc aattcggaaa accgtaactg ttctataccc
     2101 gatactttgc tggcgacgac aacttcttta ttccttttcg gatctttatt gacgtaaaac
     2161 tgtttaatgg tttctttcaa tattagtggc agatattttg ttcctgtcga agtagaatcg
     2221 aaattagata cgacaaacct gacgggtttc aaaattttgc gtttggctag tttagggttg
     2281 atattataca aagaagccag tgttttggta tattcttcgt attgataaga ttcgtatgat
     2341 tttggtttgt ttagcgtttt gtttttcacc acatttctga aaatacgaat agcagccgtg
     2401 tctttaggga tttttccctt tttagctttc accgtaaatt cttccagcat ataactgtcc
     2461 aagtgcattt tgactatgat ggaatctttc ttgaaaaacc gcttttttaa gatttcatct
     2521 ttatagccta gataagaaaa aactacggaa tcagtctttg aattcaccaa cataaaaaac
     2581 cggccggaat catctgttgt gccgccttga ttgttttcaa tggcataatt gacataagca
     2641 agcggttctt tcgtttttgc atcaatgact acaccacgca attctatctg actgtaacca
     2701 atatggaaag caagcagtaa tgtgataaaa acaaggaaat gcctaaaatt caatttatcc
     2761 ggtttaaaaa attcacacaa aaatacacat ctcaatatgt attaactgtt aatacatgtc
     2821 aatattctta ttgtacaaat tatttgccaa ttttttatca agcaaaaaag cccgcttact
     2881 ttgctttctt tttgtccttt ctttttcgac ttagtattcc agtttttttt gaggagtttc
     2941 ttttatctgc tttttcttct gtaatcttat atttctgaaa agaaccgtca gtaaatgcat
     3001 ttaaggcacg aacgtaaatg ggattttgaa gatttgttac ttgccagtat acggagctgt
     3061 tgaataaatc ctgcccgatt aatcctttta actggctttt tatggtcggt atactttgtg
     3121 accacacttc agcagaatcg ggttcagctt ttttatcttt cgcaaatgca taaaactttt
     3181 gaagcagtga atcgtctatc gagaaatttt tgatataagc atctgccgtc ggatattttt
     3241 tttgcattgc aactctgtta tcattgatat agacaagtgt aaattgatta aacgaaccag
     3301 tacgaaccaa tttttgataa tatttggaat tccatagggt atccatgggt acaaaaatat
     3361 caggagtaat accgccgccg ccgtatacta cccttttatc tgcggtaaaa tattgcgtag
     3421 aatctgtcag ttttagtttt ttctcgtcaa aaagttcccc gctttcatag cggtcttcaa
     3481 tatcttcatg atacttttct atccctttgg cataagattt ttgaatattt cttccgctgg
     3541 gcgtataata atgtgccatg gtaagcctta ccatgctcat atctggaagt gtataaccct
     3601 tttgaacaag gcctttccca tacgttcttc tacccacaat caagcctctg tcccagtcct
     3661 gcacggcacc actaacaatc tctgaagctg atgccgtatt ttgattaacc atgataacta
     3721 aatccccttt ttcaaaatcc cctgcttctg tagcattcaa atcaatacgg ggttgtgagc
     3781 gaccggaaga gtacaccact aattttccat tctccaaaaa taaatcggat aaagcatgtg
     3841 cctgattgag ataaccccct ccgttgtcgg taagatccag tattattttt ttagcccctg
     3901 cttttttaag tgaatcaata gcttgctgtg tttcttcagt agctgttgcc gaaaatcgcg
     3961 atagcttaac ataaccgata tctggtgatg ccatatactt ggcttctatg gcataaattg
     4021 gaattttatc ccggatgatt tcaaaatcca gcaaatcatt ttcgccatat cgttttattt
     4081 tcacagttac atgcgtgcct ttcttgcctc tgagtttttt aaatacgcca tcattcgtaa
     4141 tacccacacc ggctacattt gcggtgtcaa caaagatgat tttatctccg tcttgtatgc
     4201 ctactttttt agatggtcca ccttcaataa catgcacaat ttgtatagta tcctgatata
     4261 tctggaactg cacgcctaca ccttcaaaat taccttctaa tggttcgttt gctttttttg
     4321 tttcggtggc atccatgtac acagagtgcg gatcaaggct tttcagtatc tccctgatgc
     4381 cgatttccac tagtgaatca acatttacat cttctacata ataattatcc agcaagttga
     4441 ggaatgtacc cattttttgc tgatacattt gagcatttcc tgacagaccc atgcttaaaa
     4501 acagcgaaaa gagtatgccg tatttcttca ttttcatcat acgaaataaa aatacgcaat
     4561 atattgaggg taaaatgtta ttaagcctta ttttttttgc ggtaacggat gtttaataat
     4621 tcaacagcaa atgagaaggc tacggaggtg tatataatgc ctttttgcac ttcataatgg
     4681 aagccttcca taatcagtgt tacaccaatc ataattagga aagaaagtgc tagaatttgt
     4741 aaactcggat atttggtcac tattttgctt acgggttgtg taaacaacat cattacaagc
     4801 atagaaataa caacagcaat aatcatgaca ggaatttctt tgacaatacc tacagccgtc
     4861 aggatagaat ctatagaaaa tactaaattc agcaaggcaa tctgaataag taccgaaaca
     4921 aaagacgatt tcttttcgct gtttgttaca ttaaattcgt catcgtctcc tgttatcttg
     4981 tggtgtattt ctgtggttga tttatagatt aagaagattc cccccacaat gagaattaca
     5041 cctttccagc tcaattcaag attccatgcg tttggaggta agatattttt atccagccct
     5101 aaaatccatc ctaagaagaa caataagatg atacgcagga tcatggcaat aaacaaccct
     5161 aaattttgtc cgcgcttgcg gtctgcatcg ggtagctttt gggtaacaat agaaataaag
     5221 atgatattgt ctactcccaa tacaatttcc aaaaaagtaa gggttagcaa actcaaccaa
     5281 gttcccggct gggaaaaaat tgtaaaatcc ataatgcgta attaagctac aaagataaac
     5341 aagtacatac agaactcaat acaattctgg aacgaaaaaa gttccgcaga aaaacaagaa
     5401 agtacagata ccttatacta aatgtgtttt gtcaaaattc ctaaaaggag aattaagaac
     5461 aagacaagaa agtactttaa cgtttgtaaa ttttcagtca taggcaattg tcgtttcatt
     5521 tcaagtgttt taaattcagt aatcatagtt ttcctactaa atttatacaa aaacaatcat
     5581 attgacaata cctttaatgt cttttaacaa aaaacgaaca gtgtggaaat gtggaaaact
     5641 cagctcctgt cgatgtcttt atattgtaaa tcgtgctgat aataaaataa atcccattta
     5701 ccatgcgaaa attccagagc cgtgagattt tctatccagt cgccactatt gaggtaaact
     5761 gccttaccgg cctctgtttg tacttcgcga atttgcggtt tatgaatatg tccgcagacg
     5821 atgtaatcga atccgttttc aatacctatt tctatggcta catcttcaaa atcctgtaca
     5881 ttttttttaa caatagactt aactttgtct ttgatatact tggaaataaa aatctgactt
     5941 ctgccgtaac gcatgcgaat attatttacc catctgttaa gttggaaaat tttatcataa
     6001 tatctgccgc ctaattttgc cagccagcgc gcactccctc ctactgacaa atcatattta
     6061 tcgccgtgaa aaatccaatg ttttttaccg tcaatttcga ggaaaagctc atcacgaagt
     6121 tgcagaatat cgaaattaaa tcctgaaaaa ttacgcatat ggtcgtcgtg atttccggtg
     6181 aggtagtaca ccggtgtacc cttttgagcg tattctaaaa caagaaaaat attttctata
     6241 tgttctttgg ggaaatagtt agcagagaaa ttccagatgt cgagccaatc gccattaata
     6301 attaatacgc ccggatcgat actattcaga taacggttga gttcaagtgc atggcatgca
     6361 tgagtaccca gatgaatatc agagataata caaatatcta tcttgcgttt tactttcgcc
     6421 atacgatata aaaataaaac ttaattgatt aagctgttta ttaaataaga aaaaagttgt
     6481 tgacctattt gaatagtggc aaatggataa gatggatttt ttctttataa aaatactgtg
     6541 tcgttttttc aaaactctcg gtaaagtctg acacgtaaaa ctcgtgatgc gcctttggtt
     6601 caaaattaac caacccgttt tcgataagaa aatgcttgat atgcttggct acaatttgat
     6661 tggtagccaa cacatctact ttcctattgt gttttgcata ataggctact atctcttttt
     6721 tgatcagcgg gtaatgtgta caagcaagta ccagacattc gatatcagct agttcgggtt
     6781 tattgagata ttcttcaata accagatgag atatttcgcc atgagcaaat cccgattcta
     6841 tcataggagc cagcaaaggt gtggccaatt gcgatacctg aatagaggca tctctttgct
     6901 caattttatg ctgatatact tttgaattaa ccgtgcccaa ggtgcctatc acacctactt
     6961 tgcgatattt gttttttaat atataatcca ctacgggttc tatcacacta aatacgtagg
     7021 ccttttcgcc tgccatattt tgcagcattt cataagcaac tgcagatgct gtattgcaag
     7081 cgataataat tattttgcaa tctttaccca ataagaatga gccaatagtt ttggcaaaat
     7141 agcggatagt atccgcactt ttatcgccat aaggcaaatg agcagtatcg ccaaaataaa
     7201 ttaagttttc attgggcaac tcgtccagaa tggcattggc aacagttaag ccaccaattc
     7261 ccgaatcaaa taaacctata gactgatgtg catccggcat aaagcgaaga tacgagatgc
     7321 gattaaaact tttgagaatt taggacataa aaaaagaccg gaattagccg gtctttaatt
     7381 tatcaagata ttttagtgct tatttaggtg tagttcctgt ttttggagct tgaggagccg
     7441 gtttgggttc aggcagtttc aaataagatt tcaacaaagg caatacatca tcgctttcat
     7501 cagcatataa taatgaacct gcgctagagt cgaagatata tgtataaccg tttgctttag
     7561 ctactgcttt gatagcatcg ttcacttttt gcataattgg agaatacaaa tcatcttgtt
     7621 tcttttgtac tttttgttga gatgcttgtt ggaattcgtt gatgcgttgt tccaaatctt
     7681 gcaattcttt tgctgccaat tctgcttcag cttctgacat taatttcttg tcatattttt
     7741 gttgaaaatt cgtagctttt gtctgatact caacagccat ctgattagcc aaattgtcta
     7801 aatctttagc atatttatca ataacagtat cagctttagc tttttcaggc atcaaggaaa
     7861 gcaattcata agaattgaga taaccaaatt tgtttacttt ttgtgcaaag gatgttgcaa
     7921 tagctgcaat cataatcaat actacggtag ttaatttctt catttgtaaa attttaattt
     7981 tttttgtttt tattatggtt gattaattta cgcctaaacg ttttaatatt tcttcgctca
     8041 agtctaattt gggattagaa tataagatag cacttccgcc tgatgcttta tcgaaaacaa
     8101 aatcatattg tctgttttct gccagctttt gaatagcatc aaatactttg tcttgtatcg
     8161 gttgaattaa ttcctgacgt tttttaaaca aatccccgtt tacgccgaat cttgattttt
     8221 gcaaatcttt taattcattt tcagctttta ttatatcttc ttcacgttgt tttttcatgt
     8281 cttcagacaa taaaaactct tctgcctgat atttggtata catttttttc agggcttcgt
     8341 ttttttggtc aatttcagcc tgccatgctt tagcctgatc atccaacttt atttgagctg
     8401 atttgtaatc gggcagtttt tccaaaatat acttggtatc gacataagcg aaacgttgag
     8461 caaaagaatt tgtcgtcagc aatgcgaagc aacaaagtac aagaatggat aaaagttgtt
     8521 ttttcatttt atatatttta ttccggttcg aagcctaaga taaaggatat atttccgttt
     8581 ttaccgatgt agtcaaaaaa tccggaagca ttttttatcg ggcttccatt accactatca
     8641 aaacgtatgc catagtcaat accaattaaa ccaaacatcg gcaacactgc acgcacaccg
     8701 ataccaacgc ttttcttgag tttgaatgga ttgtattctt ttatactgtg ataggtattc
     8761 gctgcttcaa aaaatgccag accgaaaatg gtggctgttg gacttaatga aaacggatag
     8821 cgcaactcta ataagaactt gttataaatc gggtcaccgc cggtagatga gtaaggacct
     8881 tcatacccac gatgcgctac gatatcagtg ccaaataaag atacggtgga tggtatacca
     8941 tttccgccca cttcaaatcg ctcgaaagga gttataccga tttttttatt ataagctccc
     9001 aaaaatccga attttgccac cgtacgcaac accagcttag atttacctaa ctgctgatac
     9061 cagtcaacgt tgaacttaaa tttataatac tcaatcagtt tgtatttttc tgaaagtgtt
     9121 tttccgtcga aattattgtc ttttctaaac agagaatacg gtggtgtaaa ctggaaagac
     9181 atttccactt ttgccccacc tgtcgggaaa acaggttggt tgatagagtt gcgttgtaag
     9241 gataatttaa aattcaggtt attgaatttc ccactgtcaa tactcacatt attgatataa
     9301 tatcctaaat agttttggag aatataactg tagtaattcc cggaagcctg gaagatgaaa
     9361 tagtcatcgg gttttttcaa tcttgtaccc aatgacaccg acatacctgt ggtaatttgt
     9421 tttcctgtta cctttttgtt ttcatctaaa ttctgaatac gggaacggaa ggctgcaact
     9481 gtaaatgcat taggtttttt gccacctaac caaggctcag taaatgagaa attataattt
     9541 tgatagcgct tgccgttact ttcaatccgc aaagtcaatt gctgcccatc gcctgttggc
     9601 aatggcttcc atgcatcttt gtgaataatg tttttaagcg aaaaattatt gaatttcaaa
     9661 ccgagtgtac caatcagacc tccttgtttt cctccccagc ctgctgataa ttctacctga
     9721 tcggcagatt tttcttttac ggtgtatttg atgtcaactg taccatctat cggattagga
     9781 ataggttcca ctttagtttc ctgtgggtca aaataaccca aattggctat ttcgcgttga
     9841 gatcgaatta agtcagaacg agaaaactta tcacccggaa aagtatgtaa ttcacggcga
     9901 atgacattgt catttgtttt atcatttcct tcaatgatta cattttttat tgtggcttgt
     9961 ggcccttcgc taatgcgtac ttccaaatca attgaatcat tttctgcaga agtttcaatg
    10021 gcatttacat taaaaaacaa ataaccgtcg tcataatata atgaactgat gtcaccacca
    10081 tcaggatcca tgttcaatct tttttgcaac aactcagcat tataaatatc tcctttttta
    10141 atattcatta ccttactcag ttgttcattg gtgtatttgg agtttccttt gtattcaata
    10201 tttctgaaat aataacggtt gccttcatca atatttaatt taacactcac attgccatca
    10261 tcatttttaa tcacggtatc tgaaatgata cgtgcatctc tgaaacccag gctattgtaa
    10321 taattaatta aatttgattt gtcttcttcg tacttttctt cattaaattt cgaacctttg
    10381 aaattgatac ggaatcgctc tgcgaaaaaa tcgataaaag atttatgatt gaggttgccc
    10441 aaggtataca atgcatctac tgctttttgt ttttttgaac ggcttttatc atgcaaaaac
    10501 agttgtactt tagtgcgttc tttcgtacct ttcattgcct ttcgcaactt agctaaactt
    10561 gcgctttcta caccgttgaa ttcaatatta tttattttta ctttattgcc tttcgtcaca
    10621 tttacggtta aggctgcggc attaattaac cctttttctg cattttctac aacattcact
    10681 tgtgcattca aataagattt ttcctcaaat ttatctaata ctgtattttt tacattcaat
    10741 ttcaaggcat ccgtaagcgg ttttcctttg taataatcta atcttttcag cacatcttct
    10801 ttatcacttt ttttcaagcc atttaccgtt atactgttca gccttggtct ttctaccaca
    10861 tccacccgca ggtaaatgct atctcctacg atattggaag cgtaaacttt tacattgccg
    10921 aataatccct gacgccataa gtttttaatc agtttggtta cttcatctcc aggtacagtt
    10981 atcgcatcac cgggtttcag tccgctcaat gaacggatga tgtttttatc taaaaagttg
    11041 gtgccgctga tatccaatgc agcgagtgta taggtttttg gctcattcgc agcagcaata
    11101 gaagtcggca ggctatcttg tgccagcaat aatgcttcag cagatgcagt atcttgtgct
    11161 tttatcattc cggttttgcc catcagccca aaacaaagaa tccatatata taattgctgc
    11221 ttcatgttca taattaatct tatttttctt caagttgttc agtcgtcata ccaaatcttc
    11281 tttctcggtt ttggaaatca tatatagcat gaaataaatc ttctctgtta aaatcaggcc
    11341 agaatttttc ggtaaaataa aactccgtat aggccatttg ccacagcaaa aaattactta
    11401 ctctgcattc tccgctggtg cgaatcatca gctcagggtc gggcataccg gcagtaaata
    11461 aatttgcagc aaggaagttt tcatcaattt cgttcaaatt aacaaaacca cttttatatg
    11521 tttgtgcaat tttctgaaca gcctgtataa tctcatctct ggaaccataa ctcaaagcta
    11581 gtgtcagcgt catgcgtgta ttattttgag tttcggagat ggctttcatc aatgccttac
    11641 ggttggattc gggtaaggtg ttgatattcc cgattgcatt taatctgata ttgttttttt
    11701 gcagtgtttt tatttctttg ctgatagtca aactcaataa ttccatcagt gctgaaactt
    11761 ccttttgcgg acgactccaa ttttcggtgg aaaatgcata taaagtcaga taattcactc
    11821 ctatttctgc acatccttca gttacttcac gtacggcttt tactccgttt ttgtgcccga
    11881 agatgcggtg tttgccctga gatttcgccc acctgccgtt gccgtccatg atgatggcta
    11941 tatgtttagg caatgccaga caatcaagct tatttttata tgattccgga ttcgtctgca
    12001 tttaaaatac tttgggacat ttaaaagtga taaacgtgta ggtaagccct gcaccgataa
    12061 ataaaaatct gtctctgtcg ttgctggtgc ctcgctgctt gccaaaaaca ccaatttttc
    12121 cggctgattt gtcagctata tccggtccga cattttcgcc attgataaaa taattgacat
    12181 cagttggttc tgcataaaac ttgctgacat catccagata atcgttaaag gtaagtctgc
    12241 cgctgatttc aacattgagt gctaaatgct gtgtgagcgc aattttaaaa cctccgccat
    12301 aaggcatggc aaatgcatag taattatatc ctctgttttt ggtctgaata ttggcagact
    12361 tttgcccttc agtaccgaca gattccaact gaatacgctt accgtcatag cgggtgatgg
    12421 gattgaaata aaacatactc actccggtaa acaaataagg agtccatcgt ttggaggtgc
    12481 cgtctttata gtcgtaagta gagtacttga agaaattgat ttcgtaggtg attgccagtt
    12541 ctgcaatttg agagttaaaa tcaagattgc gagcctgcaa gtacggatag ccttttatcc
    12601 ggctgtcgtc agcagagaga aaggcataat tacctccggc ttttactgac attctgcctt
    12661 taccaaaatt ataacgatag aaaactcctg tagcccaacg tgcattcttg aaagaataag
    12721 ttggatttaa atcgccgagg tatacagcag ttccacccca tgctcccact tcattttctt
    12781 gtgcaagcag gggcaagttt agcataatgg ctaaaataag ataaactatt tgctgtctct
    12841 ttttcaaaag aggttaaaat tttggtgtac gaagatataa tattaacgtt gaatatcgaa
    12901 atataagggg gattataagg ttttattaat gaattgccgg atgtttttca cattttttaa
    12961 gatggtcagg aaaattgcat aaaatatgag gtatttgact aaagtatcga aaatatatat
    13021 tatatacgac gcaaattctg cttaaccaat ttgtaacaac ttactgttta caaaatggtc
    13081 ttaaaatatt ctatcgtcaa gggcaatcct tgctcaaatg aatactgggg catatagccc
    13141 agtttagttt ttgcatttga aatatcggcg agcgaatcgc ggatatcgcc tgcacgcggc
    13201 tcacggtgta tggcaggtat atcacttttc aaatagtgcc gaatggcatc atacatctgg
    13261 ttgatggtaa atcgctcgcc aaatgccacg ttgtatgcct gattatacgc atcttgattt
    13321 tcaactgtaa gtgcttttat attagcttgc actgcattgt gtacaaatgt aaaatcccgg
    13381 gtttgctcac cgtcgccatt gatatacggg gcttcatcgt tcagcaacga tttaataaac
    13441 aagggcatca cggcagcata ttccccatca gggtcttgtt taggtccaaa tacattgaaa
    13501 tagcgcaaac caataatttc catgttataa catctcgaaa aaacagaagc ataaacttca
    13561 ttagtaaact tagttacagc gtacggagac agcgggtttc caacacgttc ttcacgcttt
    13621 ggtaaattgg gctcatctcc ataaacggat gatgaagaag catacacgaa cctttttgtc
    13681 cctgcatttt tcgcggcagt cagcatgttg acaaacccat ttacattcac ttcattagaa
    13741 gttacggggt cttttatgga gcgaggcacc gagccaatgg cggcctgatg acaaaccata
    13801 tccatcctat cgcaagcctt ttcacagtcg gcaaaattgc ggatatcacc ttctaaaaat
    13861 tcaaaattgg gatttgttct gaattcttcc agattcttca ggtttccagt tgccagatta
    13921 tccagcactc gcacctttgc accgtgcgtt aataaatatt cgacaatatt tgaacctata
    13981 aatcccgcac cacctgttac aacaattttt ttatcctgaa gaggtgataa gtgaaacgcc
    14041 tgcttataca tgtagcaaaa atacgaaaag tgattttagt aacgctattt acaatataat
    14101 taagtaaaca tttttctcag caacctgagt tctgatgaaa tgctttaata taaatggtta
    14161 tttatttatt gaattcagcc attccgggaa aagcgttaca atttataaaa tagtagattt
    14221 tttattaaga tcctcttcct attttattct tcctccttgt taagtggaaa aagaagaaat
    14281 caactatttt ttaccttacc tgtctaaccc aaattcagga tattgaaatg agtatacata
    14341 aaaaaagagt cgggttaact actcccgact ccaactaact aactaactaa ccaaatctta
    14401 ctttaaaaaa gtggcaaagc taaatttttt gtggtaaaag ctggagattt ccgaaataaa
    14461 agaggat
//
LOCUS       DAOQII010000033        14380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2030_length_14380_cov_5.039791, whole genome shotgun sequence.
ACCESSION   DAOQII010000033 DAOQII010000000
VERSION     DAOQII010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14380
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_14380_cov_5.039791"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="PLF48_02830"
     CDS             <1..2302
                     /locus_tag="PLF48_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48288.1"
                     /translation="DMDIQLVAPDGTVYDLILDRCGGYFFHLNICFSNKAGTNVTSAV
                     CPGSGGEMAGNYIAQGNWTGLNGEGINGNWTLRFTDDSWALDPCTLFGWNIEIKKTMT
                     VSASPIPGVNHGDLHIINNDPFKYGSQVFWLTPVTFINYNGTTLTSDSCYDYGTPVKI
                     TLLERVTTPTIITTCAAPGDGSNGVTIRVTSPTGGMPGLPSPTAADTFRVVGSGAASG
                     IVFPTRPVGESELSNSFTVLDGQAWNVRFVDNNGCESEIGGTYNQPNLGALQIDTNVC
                     DGATVAFSTTNPIPLFSQYRVLLDFDSYPQDVSWFIYDGNNQVVASGGGYGTTVGAAT
                     YTTPATINPNNGPYRFVLYDGFDDGFGSGGGSTNNGGLNTLNFIRVVEVHADGTVDTL
                     FSQNYAFCTPIYCVGPTASVFGQLDIPLGTPNGTFSTGVSVSLENNRTCTGTAVAGAI
                     TMNQDGTGTVNTNAAGVNAGSSYSVQYQYTDQYGCVKQICGPLDVFPNITLAPTVNCA
                     TSPPTVSVNATCTGCNATYVAEYSYNGTTSWTTSTTGNFQDLYIFAHVKNVSTGVIGC
                     EVQSTKLGTCATVLPIEFIYAKAEPVNNEYIKVSWATASEYNSDRFDIWRSEDGINFA
                     KVGTVPASGNATQQRNYFYDDYRVQKGITYYYKIIEVDMDSREQHSNVVNAKLTIDAL
                     EVTGIYPNPVKESATIEVQAKEKDNLQMIVYNDLGQIMLQQDVELKAGTNSIVVDMKT
                     WANGIYYFNLKNDNIKPIIKKVLKKD"
     gene            2408..2713
                     /gene="gatC"
                     /locus_tag="PLF48_02835"
     CDS             2408..2713
                     /gene="gatC"
                     /locus_tag="PLF48_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNF48289.1"
                     /translation="MNVNDELIDKLSKLAKLDFTTAESKAEIKADLSNILSFMEQLNE
                     LNTDNIAPLVYINEEVNVMRTDEVHYPISKSEALQNAPLKNEDYIKVPKFVKGTKYE"
     gene            2718..3443
                     /locus_tag="PLF48_02840"
     CDS             2718..3443
                     /locus_tag="PLF48_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF48290.1"
                     /translation="MQAPVIALNQITRKFVLGDEIIQALKGIDLTVGKNEYVALMGPS
                     GSGKSTLMNILGCLDTPSSGAYFLDGKDVSKASDNELAEVRNKQIGFIFQTFNLIPRL
                     STLENVALPLIYAGWNKKDRDERAIEVLHQVGLGDRMKHKPNELSGGQRQRVAVARAL
                     VNHPSIILADEPTGNLDTKTSYEIMALFEEIHRNGNTIVVVTHEEDIALYTHRIVRVR
                     DGLIESDELNQQIRKVSPSVNMK"
     gene            complement(3426..4013)
                     /locus_tag="PLF48_02845"
     CDS             complement(3426..4013)
                     /locus_tag="PLF48_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNF48291.1"
                     /translation="MEFSLKDLSTVTLILFAIIDILGAVPVLLDIRAKVGDVNAKYAT
                     LVSGFIMILFLFIGKTLLKLIGIDVASFSLAGSIVIFLVGLEMTLNRDIFKTATSSAK
                     TSSVVPVAFPMIAGAGTLTTLLSLRSEYDIYTILIGILINLVVIYLVIRFIPKIEKML
                     GDTGINILRKIFGVILLSIAIKLFSKGVIQLFHIH"
     gene            4321..5754
                     /locus_tag="PLF48_02850"
     CDS             4321..5754
                     /locus_tag="PLF48_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF48292.1"
                     /translation="MKKSIVLFSALLILMSSVALGAKKPPRNKNSAVGNNRFSIGVGM
                     EIADYYTPALKKMSLFKKPINFGPRITGWYNPNSSVAVGLSLASHAFSSKNTDPTLPV
                     INQYNLLYGAELAYKFNNGYIMKEDAAFAPYLFARLQGSWAQDKFQQNYNGFGIPVGA
                     GINFKIANNVALNVNGGYNFGIKNNDSHIFFGAGLMADLGKGKDVAVDTVPVVVEKPI
                     DTDGDGIIDLDDACPTVPGIAQFNGCPDTDGDGIEDSKDECPTVPGLAEFNGCPDRDG
                     DGIPDMKDACPDVKGIAQFGGCPDPDRDHDGIVNEKDKCPDVPGSFIAQGCPDRDGDG
                     VMDDQDKCPDVAGPASNQGCPELKEEIKKKLAFAARNIQFETGKSVIKPVSYKILDEV
                     ASILNEYPYYDVNVDGHTDNVGDDAKNMTLSQARAEAARTYLINKGVAAGRMVATGYG
                     ETKPIADNKTAAGRAQNRRVEFNLIFK"
     gene            5827..6516
                     /locus_tag="PLF48_02855"
     CDS             5827..6516
                     /locus_tag="PLF48_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNF48293.1"
                     /translation="MDDSKYTFSRGERNGILLLVLMIIFGIFAKSYIIQLFEKESTVS
                     AQEKENIIALQNQIAEAKDRSNTASYSPKYNTSTYSESKTNNVPAAVNEHKPFTLEVN
                     SATAEEYEKLYGIGKVLSVRIVTFRDKLGGFYSVNQVKDVFGVEDSVFQHFKSHLTIK
                     PKVPKKININSATYEELTANPYFFSTVAKQIIGYRTKVKPFTTIEDVKNLYFVRDHPE
                     HFDKMKPYIEI"
     gene            6577..7725
                     /locus_tag="PLF48_02860"
     CDS             6577..7725
                     /locus_tag="PLF48_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF48294.1"
                     /translation="MNFQVSEQQQLIAQTVRDFAEKNIRPHVMEWDEAQHFPAETLHK
                     LGELGLMGILIPEEYGGAGLGYFEYITALIELGKVDSALTLSVAAHNSLCTNHIYKFA
                     NEEQRKRWIPKLATGEWIGAWGLTEPNTGSDAGRMKCVAKKEGNEWVINGTKNFITHG
                     ISGNVAVVIVRTGELLDSHGMTAFVVERGTPGFSGGKKENKLGMRCSETAEMIFDNCR
                     VPEENMLGKEGEGFIQAMKILDGGRISIAALSISIAVGAYEAAVKYAKEREQFGKPIA
                     DFQAIGFKLADMATQIEAAKLLTFQAADMMMRGEKMTKESAFAKYYASEIACKVANDA
                     VQVLGGYGYIKDFPVEKFYRDAKLCTIGEGTSEIQKLVISREILKGNI"
     gene            complement(7860..8456)
                     /locus_tag="PLF48_02865"
     CDS             complement(7860..8456)
                     /locus_tag="PLF48_02865"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNF48295.1"
                     /translation="MMRIAIFLSGGGSNARAICSYFKDHSDIQVALLLSNKEKSGTQQ
                     IATDYNIPYFIFNKEQFKEGDVLLNALEKNKIDYIILAGFLWLIPDYLLKNFPDKIIN
                     IHPALLPKYGGKGMHGMHVHQAVWENKETESGITIHLCNEHYDDGKILAQKTTTITEE
                     DTPQQIAQKVLHLEHQYYASTIEKYINLGSGQTFQLKK"
     gene            8517..9629
                     /gene="gmd"
                     /locus_tag="PLF48_02870"
     CDS             8517..9629
                     /gene="gmd"
                     /locus_tag="PLF48_02870"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNF48296.1"
                     /translation="MKKAIITGITGQDGAYLAELLLNKGYEVHGIKRRTSLFNTDRID
                     HLYEDPHIENAHFKLHFGDLSDSTNLIRIIQDVQPDEIYNLGAMSHVKVSFDTPEYTA
                     NVDAVGSLRLLEAIRLLGLVNKTKLYQASTSELYGKVQEIPQSEKTPFYPRSPYAVAK
                     MYAYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRAVSKIALGLQDKLYLG
                     NLNAKRDWGHAKDYVEAMYLILQQPEPDDYVIATGKTTEVREFVKMAFKELGIEVEFK
                     GSGIDEKGYIASCSNPDYQVEIGKEVVAIDPAYFRPTEVDLLIGDPTKSKTKLGWIPK
                     HDLASLVKDMMKADIKLFERDKYLIEGGHKIFNYHE"
     gene            9698..10651
                     /locus_tag="PLF48_02875"
     CDS             9698..10651
                     /locus_tag="PLF48_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNF48297.1"
                     /translation="MDVNSKIYVAGHRGMVGSAIVRKLKAEGFKHILTATSKELDLTN
                     QADVDVWFEKNKPDYVFVAAAKVGGIYANNTYRADFLYQNLMIECNVIHASYLNNVKK
                     LLFLGSTCIYPKLAPQPLNEDSLLSGFLEETNEPYAIAKIAGIKLCESYKRQYGCNFI
                     SAMPTNLYGPNDNYDLNKSHVLPALIRKFHEAKMNNLPEVVMWGTGSPYREFLHADDL
                     ADALFFLMENYEGEQFVNCGSGEEITIKDLALLVKDIVGYDGNIVHDTTKPDGTPRKL
                     TDVSKLHAMGWKHKISLQDGVRKVYEDFARNYESIIQKYAN"
     gene            10641..11615
                     /gene="argC"
                     /locus_tag="PLF48_02880"
     CDS             10641..11615
                     /gene="argC"
                     /locus_tag="PLF48_02880"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNF48298.1"
                     /translation="MQTKIRAGIVGASGYTGGELIRLLVQHPNAEINFAYSRTHHGKA
                     LATIHSDLIGDTTLQFTDSINQDVDVVFTCLPHGETGTFLKNNPISDKVKIIDLSNEF
                     RLKNTASGFVYGLPEINKNKIKSAQKIANPGCFATAIQSALLPLAKAGLLQSAIHVSG
                     TTGSTGAGNKLTASSHFTWRNNNFSTYKAFTHQHLDEIGESVLQLQPSFSSAINFIPH
                     RGNFTRGILITAYTEIESSLDEIKKIYNAFYSDAPFVFISDVNIDIKQVVNTNKCLLY
                     LEKQGNQLLVTAVLDNLLKGASGQAVQNMNLMFGFDEMAGLRLKPLAY"
     gene            11763..13031
                     /locus_tag="PLF48_02885"
     CDS             11763..13031
                     /locus_tag="PLF48_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF48299.1"
                     /translation="METIHTDVVIIGAGFTGLACAYYLQQKGYSVKVVEVSSSSGGRA
                     RTDNVNGFLLDRGTHFYHHSTTELKKIISIDKLGLKNLYPGYLLRYENGFHLFTNPLY
                     KTFDTISTVFAPNATVADKMRLFGLYVKLKTLPYSTLVKENESSTYEYLSKNGFSSKL
                     IDTLFRPLVAANIFDYNLQSSSRFSKVYLKSLFQEHVALPKEGIGSVAKHILDKLKPD
                     TVMFNTKAKRIHQDGITCSNGKTSRIVSKFTVIATNPIDTNLLMQSDVLPTECSHVST
                     LYFYSENPPLSKPVVVLNGNSNGMVNHIFVPTNVHPKMAPEGKHLIAVSVVKTNDLDD
                     EELPEAVMKELTEWFGLKVMDWTHLKTYHIKYAMPFKPILDEVTYYKKINDTVYACGD
                     SLSVGSMEAALRSGRETAQALIKELKSIPA"
     gene            complement(13033..14241)
                     /locus_tag="PLF48_02890"
     CDS             complement(13033..14241)
                     /locus_tag="PLF48_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNF48300.1"
                     /translation="MKISTYRLLFLLYFSCSVSSVFGVNDSFKVAKLSPYAKISLLTV
                     GRSDAEVWQKFGHTAIRIKDSIKGWDIVYNYGTFSFGNSESEFIWNFVKGRLLYYLTI
                     DMYPDFIAEYIEENRSVSEQILNLDTIQKQKLFEALTFNAREENKYYKYDFLFDNCST
                     RPRDKILSLFNTVQFQKDADDDVSYRKLIRKYANDSWLDFGMDLLIGARTDKKAEFGR
                     TFLPDELMQSFDGATADGKKLTLSNRLILDTVPEHMNKNWLTPSLVFWFLFLVLFLLQ
                     IYYQLLYRYKIIPVIYLCILGLFGWLFIFMWFFTDHKTTKYNLNIIWAMPLLLPFIVF
                     NLKKSVPNAIIIFTKAYRVLLIFLILLWWLNPQKYNLAVLPLILLAILSASVLLPIPK
                     SGDFSKRLFP"
BASE COUNT         4759 a         2443 c         2893 g         4285 t
ORIGIN      
        1 tgatatggat attcaattag tagctccgga tggaactgtt tatgatttaa ttttagacag
       61 atgtggcggt tatttttttc atcttaatat ttgtttttca aacaaagcag gtactaacgt
      121 aacctctgca gtttgcccgg gttcaggagg ggagatggct ggaaattata tagcacaagg
      181 aaattggaca ggactaaacg gagaaggaat taatggtaac tggacattga gatttacaga
      241 tgattcatgg gcgctggatc cttgtacatt atttggctgg aatatagaaa taaaaaaaac
      301 tatgacggtt tctgcttcac caattcctgg tgtcaaccat ggcgacctgc atataattaa
      361 taacgatcct tttaagtatg gaagtcaagt gttttggtta actccagtaa catttataaa
      421 ttataatgga actaccttaa cttctgattc ttgctacgat tatggtacac cggttaaaat
      481 tacattatta gaacgcgtaa ctacgcctac tattattaca acttgcgctg ctcccggaga
      541 cggaagtaat ggtgtaacaa ttagagtgac ttcaccaact ggaggtatgc ctggattacc
      601 aagtccaaca gcagcagata catttagagt agttggttct ggtgccgcaa gtggtattgt
      661 atttcctact cgtccggtag gtgagtccga gttgtcaaat tcatttacag tactagacgg
      721 tcaagcttgg aatgttcgtt ttgtagataa taacggttgt gaatcagaaa taggaggaac
      781 ctataatcaa cctaatttag gtgctttaca aattgatacg aatgtatgcg atggtgctac
      841 tgttgcattt tcaacaacaa atccaattcc attattttct cagtatcgcg tattattaga
      901 ttttgactcc tatccgcaag acgtttcttg gtttatttat gacggcaata atcaagtggt
      961 agccagtggt ggtgggtacg gtactacagt tggtgcagcc acttacacta cacctgcaac
     1021 aataaaccca aataatggtc cttatcgttt tgtactatac gatggatttg acgatggttt
     1081 cggttctggc ggaggaagta ccaataacgg tggattaaat acactcaatt ttataagagt
     1141 ggtggaagtt catgctgatg gaacagttga tacacttttc agccaaaatt atgcattctg
     1201 cacgccaata tattgcgtgg ggccaactgc ttctgtcttt gggcaattgg atattccttt
     1261 gggtacacca aacggtacat tttcaacagg tgtgtctgtt tcgcttgaaa ataacagaac
     1321 ttgcacagga actgcagtag caggagcaat tacaatgaac caagatggaa caggtacggt
     1381 gaacacgaat gccgctggag tgaatgcagg ctcatcgtat tcagtccagt atcaatatac
     1441 tgatcagtat ggttgcgtta aacaaatttg cggaccgtta gatgtctttc ctaatattac
     1501 attagcacca actgtaaatt gtgcaacttc accaccaacg gtttctgtaa atgctacatg
     1561 tacaggttgc aatgctactt atgttgctga atattcttat aacggcacaa catcctggac
     1621 aacatcaaca acgggtaact tccaggattt atacattttt gctcatgtga aaaatgtttc
     1681 tacaggagta ataggatgtg aagtgcagag cactaaatta ggtacctgtg caacggtatt
     1741 gcctatagaa tttatttatg caaaggctga gccggtaaat aatgaatata tcaaagtttc
     1801 ttgggctact gcttctgaat ataattcaga cagatttgat atttggcgca gtgaagatgg
     1861 aattaatttt gcaaaagtgg gtacagttcc ggcttcagga aatgctactc aacagcgaaa
     1921 ttatttttac gatgattata gggtacaaaa gggcattacc tattattaca aaatcataga
     1981 ggtagatatg gatagcagag agcagcacag taatgtggta aatgctaaat taacgattga
     2041 tgcattagaa gttacgggta tttatcctaa tccggtaaaa gaatcggcta ccatagaggt
     2101 acaagctaaa gaaaaagata atttgcagat gattgtttat aacgatttag gtcagattat
     2161 gttgcagcaa gatgtggaac ttaaagcagg aactaattca attgttgtgg atatgaagac
     2221 ttgggctaat ggtatttatt atttcaacct gaaaaatgat aatatcaaac cgattatcaa
     2281 aaaagttttg aagaaagatt gatttaataa ataaatgaaa tgccggactt tatgtccggt
     2341 attttttttc aaacaaaaat cttttaatcc ttacttttgt aatgaaattt actttaatga
     2401 tactgatatg aacgtaaatg acgaactgat tgataaattg tcaaaattgg cgaaactgga
     2461 ttttactact gctgaaagca aagcagagat aaaagcagat ttatctaata ttttaagttt
     2521 tatggaacaa ctgaatgaac tgaatacaga taatatagca ccattggttt atatcaatga
     2581 agaggtgaat gtaatgcgta ccgacgaagt gcattatcca atcagcaaat cagaagcatt
     2641 gcaaaatgcc cctctgaaaa atgaggacta tatcaaagta cctaagtttg taaaaggaac
     2701 aaaatacgag taagcacatg caagcgcctg taatagcatt aaatcaaatt acgagaaaat
     2761 ttgttttggg agatgagata atccaagctc tgaaaggaat agatttaact gtcgggaaaa
     2821 atgaatatgt agccttgatg ggcccttcag gatctggtaa atctacactg atgaatattt
     2881 tgggctgttt ggatacgccc agttccggtg cgtatttttt agatggcaaa gatgtgagta
     2941 aagcatctga taatgaactg gcagaagtac gcaacaaaca aatcggtttt atcttccaaa
     3001 cttttaattt aataccacga ctgtcaacct tggaaaacgt agcattacca cttatttatg
     3061 ccggatggaa taagaaagac agagatgaac gtgccataga agtattacat caggtgggct
     3121 tgggcgacag aatgaaacat aagccgaatg aactctctgg aggacaaaga caaagggtag
     3181 ctgtagcccg tgcactcgtc aatcaccctt caattatact ggcagatgag ccaacgggta
     3241 atttagatac caaaacttcg tatgaaatca tggctttatt tgaagagata catcgaaacg
     3301 ggaatacgat agtagttgta acacatgaag aagatatagc tctttatacc caccgcatcg
     3361 tacgcgtacg tgatggctta atagaaagcg acgaactcaa tcaacagatc agaaaggtaa
     3421 gcccatcagt gaatatgaaa taactggatg acgccttttg aaaaaagctt aatggcaatg
     3481 gataacaaaa taactccaaa aatcttgcgt aaaatattaa ttccggtatc gcccagcatt
     3541 ttttcaattt ttggtataaa tcgaatgact aaataaatca ctaccagatt aattaaaatg
     3601 ccaataagta tggtataaat atcatattca gagcgaagcg acaacaaagt agtcagagta
     3661 ccggcgcctg ctatcatcgg gaatgcaacc ggcacaacgg aactcgtttt tgcagatgaa
     3721 gttgcagttt taaaaatatc tcggtttaag gtcatttcca atcctactaa aaaaatcact
     3781 attgaacctg ccaaggaaaa tgatgccaca tcaataccaa taagttttaa aagggttttg
     3841 ccgataaata aaaacaaaat catgataaaa ccggaaacca atgtggcata ttttgcattt
     3901 acatcgccta ctttagctct gatatccagc aatacaggta cagcacctaa gatatcgatg
     3961 atagcaaata aaatcagtgt gacagttgaa agatccttta aactaaattc catggcacaa
     4021 aggtctgtat tttattaaga tatggcgtga tgaaagtttt atacaaagat taaattacat
     4081 tttaaaatcg ctccattctg acttaaaata aaattaaaag aagggttttt acctaaaaca
     4141 caagattaaa ttatccataa tagcaatgga tggcttaaat tatcaacatt atgggaaatt
     4201 tcaatgtaac agtagtagga taaacaaaaa aaaaatctaa atttgcattt tattaccttt
     4261 aaccagtaaa ttaaacaaaa ttaacattat ttttgtagat aaatttaaat caaagtcttt
     4321 atgaaaaaaa gtattgtatt atttagcgca ctacttatct taatgagctc cgtagctttg
     4381 ggtgcaaaaa agccacctag aaataagaac agtgcagtag gtaacaaccg tttcagcata
     4441 ggagttggga tggaaatagc agattattat acacctgctt tgaaaaaaat gtcattattt
     4501 aaaaaaccga ttaatttcgg tccacgtatt actggttggt acaatccgaa cagctcagtt
     4561 gccgtaggtt tatcacttgc atcacatgca ttttcttcta aaaatacaga tccaacatta
     4621 cccgtaataa atcaatataa tttgttatat ggtgcagaat tggcatacaa attcaataat
     4681 ggctacatta tgaaagaaga tgcagctttc gctccatatt tgtttgctcg tttacaaggt
     4741 tcttgggcac aggacaaatt ccaacaaaac tacaatggtt ttggtattcc tgttggtgct
     4801 ggaattaatt ttaaaatagc taacaatgtt gcattgaatg taaatggtgg ttataatttc
     4861 ggaattaaaa ataatgacag ccacattttc tttggtgcag gcttaatggc tgaccttggt
     4921 aaaggtaaag atgtagccgt tgatactgtt cctgttgttg ttgaaaaacc tattgataca
     4981 gatggtgatg gcattattga cttagatgat gcatgtccta ctgttcctgg tatagctcaa
     5041 tttaatggtt gccctgatac tgatggtgac ggaattgaag acagcaaaga cgagtgccct
     5101 acagtgcctg gcttagctga atttaacggt tgccctgaca gagacggcga cggtattcct
     5161 gatatgaaag atgcttgtcc agatgtaaaa ggtattgctc aatttggcgg ttgtccggat
     5221 ccggacagag accatgacgg tatcgtgaat gaaaaagata aatgtcctga tgtaccgggt
     5281 tctttcatcg ctcaaggttg tcctgacaga gatggtgacg gtgttatgga tgatcaagat
     5341 aaatgccctg atgttgcggg tcctgcttct aaccaaggtt gtcctgagtt aaaagaagaa
     5401 atcaagaaaa aattagcttt cgctgctcgt aatattcagt ttgaaacagg aaaatctgtt
     5461 atcaaaccgg tatcttacaa aattttagat gaagtagctt ctatcttaaa tgaataccct
     5521 tattatgatg taaatgtaga tggtcatacc gacaatgttg gtgatgatgc taaaaacatg
     5581 acattatctc aagcaagagc agaggctgcc agaacttact taatcaataa aggtgttgct
     5641 gcaggtagaa tggttgcaac aggttatggc gaaactaaac caattgctga taacaaaaca
     5701 gcagctggaa gagctcaaaa ccgtcgtgta gaatttaact tgattttcaa ataatcaatt
     5761 aaaattgtat aaatttttaa gagtccgcaa aagcggactc ttttttttat tatatttatc
     5821 cttagaatgg atgattcaaa atataccttt tcccgtggtg aacgtaatgg catcttgctg
     5881 ttagtgttaa tgataatttt tggcatattt gctaaaagtt atatcattca gcttttcgag
     5941 aaagaatcaa cggtgtctgc acaagaaaag gaaaacataa ttgcactaca gaatcaaata
     6001 gccgaagcca aagaccgctc taatactgct tcttattccc caaaatataa taccagcact
     6061 tattcagaat caaaaacaaa taatgtacct gctgcagtaa atgaacataa gccatttacg
     6121 ctggaagtga acagtgctac agctgaagag tatgaaaagt tatatggtat tggaaaagtt
     6181 ttgtcagtcc gtatcgttac ttttcgggat aaattaggag gtttttattc cgttaaccag
     6241 gtaaaagatg tattcggagt ggaagactct gttttccagc attttaaatc acatctcact
     6301 atcaaaccta aagtaccaaa gaaaattaat atcaactcag caacttacga agagctcacg
     6361 gctaatcctt actttttttc aacagtggcc aagcaaatca ttggctatcg tactaaggta
     6421 aaaccattta cgactataga agatgtcaaa aatctttatt ttgtcagaga tcatcccgaa
     6481 cattttgata agatgaaacc ttatatcgaa atttaattag cggtggtgta gttcgttttc
     6541 tacatgcaaa atattacctt tgcataaatt ttaagtatga attttcaggt ttcagagcaa
     6601 caacaattaa tagcacaaac agtaagagat tttgcagaaa aaaatatccg tccgcatgtt
     6661 atggaatggg atgaagcaca gcattttccg gctgaaacac ttcacaaatt aggagaactg
     6721 ggtttgatgg ggatcttaat tcctgaagaa tacgggggcg caggtttagg ttattttgag
     6781 tacatcacgg ctttaatcga attgggaaaa gtggacagtg cacttacatt atctgtcgct
     6841 gcgcataatt cgctttgtac caatcatatt tataaattcg ctaacgaaga gcaaagaaaa
     6901 agatggattc caaagctggc aacaggggag tggataggtg catggggatt gactgagccg
     6961 aataccggat ctgatgcagg aagaatgaaa tgtgtggcaa aaaaagaagg taatgaatgg
     7021 gtaattaatg gtacgaaaaa ttttataacc catggtatca gcggcaatgt tgcagttgta
     7081 atagtccgta ccggcgaatt attagacagc catggtatga ctgcttttgt tgttgaacgt
     7141 ggaactccgg gatttagcgg gggcaaaaaa gaaaataaat taggcatgcg ctgctctgaa
     7201 acggctgaaa tgatatttga taattgccgt gtgcccgaag aaaatatgct gggtaaagaa
     7261 ggtgaaggct ttatacaagc tatgaaaatt ttggatggtg gacggatttc tatcgcagcc
     7321 ttgagtatca gtattgccgt aggggcttac gaagccgctg taaaatatgc caaagaaaga
     7381 gaacaattcg gtaagcctat tgctgatttt caggccattg gttttaaatt agctgatatg
     7441 gctacacaaa tagaagcagc aaaattattg acatttcaag cggctgatat gatgatgcgc
     7501 ggtgaaaaaa tgaccaaaga gtcagcattt gctaaatatt acgcttcaga aattgcgtgt
     7561 aaagtggcaa acgatgccgt gcaagtatta ggcggatatg gctatattaa agattttccg
     7621 gtagaaaaat tttatcggga tgccaaatta tgtacgatag gagagggaac ttctgaaatt
     7681 caaaaactag taattagccg ggaaatactg aaaggaaata tttaattcta ttcagtagaa
     7741 ttattatcat gggttcgggt ttagaaaaat aaaatgttaa aatagttgat ttctcacatt
     7801 ttcctccaac ttaacaagga ggaggaatta aaaggaggag gttcatgata aaaaatcaac
     7861 tattttttta attgaaaagt ttgtcccgaa cccagattaa tatatttttc tatcgtactt
     7921 gcatagtact gatgttctaa gtgcaatact ttttgagcaa tttgctgagg tgtatcttct
     7981 tctgttatcg tggtagtttt ttgagctaat atttttccgt catcgtaatg ttcattgcat
     8041 agatgtatgg taattccgct ttcagtttct ttgttttccc atactgcttg atgtacatgc
     8101 ataccatgca tgcccttgcc accatattta ggcaataagg caggatgtat attgataatt
     8161 ttatcaggaa aattctttag caaataatcg ggaatgagcc aaagaaaacc ggctaaaatg
     8221 atataatcaa ttttgttttt ttctaatgca tttagcaaaa catcaccctc tttaaattgt
     8281 tctttattaa aaataaaata aggaatgtta tagtctgtag ctatttgttg agtacctgat
     8341 ttttctttgt tactcaataa tagtgcaact tgaatgtcac tatggtcttt gaaatatgaa
     8401 caaattgcac gggcattgct gccaccgccg gagagaaaaa ttgctatgcg catcatactt
     8461 ttatccgtta aaaaaccgaa aaataatgta tttttgtgaa tctagtttat taaaaaatga
     8521 agaaagcaat tataaccgga attaccggac aagatggggc ttatttagcc gaacttttat
     8581 tgaataaagg ttatgaagta catggtatca aacgccgaac ttctttattt aacacagaca
     8641 gaattgatca tttatacgaa gatccgcata tcgaaaatgc acatttcaaa ctgcatttcg
     8701 gcgatttgtc tgactcaacc aatttaattc gcatcattca agatgtgcaa ccggatgaaa
     8761 tctataattt gggtgccatg tcgcatgtga aagtatcttt cgatacacct gaatataccg
     8821 ccaacgtaga tgctgtaggg tcattgcgtt tgctcgaagc catacgttta ttgggcttgg
     8881 tgaataaaac caaattgtat caggcgtcta catctgaatt gtatggcaaa gtgcaggaaa
     8941 tacctcagtc ggagaaaacg ccgttttacc cgcgttcacc ttatgctgtt gctaaaatgt
     9001 atgcgtactg gataaccgta aattaccgtg aggcttatgg tatttatgcc tgcaacggca
     9061 tcttgtttaa tcacgaatca ccattgagag gagaaacttt tgttacccga aaaattaccc
     9121 gtgctgtttc taaaattgct ttagggttgc aggataaatt atacttaggt aatttgaatg
     9181 ctaaaagaga ctgggggcat gctaaagatt atgttgaggc tatgtatttg attttacaac
     9241 aacccgaacc ggatgattat gtgattgcca caggcaaaac gacagaagtt cgcgagtttg
     9301 tgaaaatggc atttaaagag ttgggcattg aagtagagtt taaaggtagt ggcattgatg
     9361 aaaaaggtta tatcgcttct tgttcaaatc cggattatca ggtagaaatt gggaaagaag
     9421 tagtcgccat tgacccggct tatttccgcc ctactgaagt ggatttgttg attggagatc
     9481 ctaccaaatc aaaaacaaaa ttaggctgga ttcctaagca cgatttagct tctttggtga
     9541 aagatatgat gaaagcagat atcaaattat tcgaacgaga taaatacctc attgaaggtg
     9601 gacacaaaat ctttaattat catgagtaat ttctagtcag tgcatattga caatagtttt
     9661 ttgttgggtt tttacagaga cagtataaat aaatttcatg gatgtcaatt caaaaatata
     9721 tgtagcagga cacagaggta tggtaggttc tgccatcgta cgaaaattaa aggctgaagg
     9781 atttaagcat attctcactg ctacatcaaa agaattagac ttgaccaatc aagctgatgt
     9841 ggatgtctgg tttgaaaaaa ataaacccga ttacgtattt gtagcggcag ccaaagtagg
     9901 cggaatttat gccaataata cttatagagc cgatttcttg taccagaatt tgatgataga
     9961 atgcaacgtg atacatgcat cttatctaaa taatgtaaag aaacttttgt tcttaggaag
    10021 tacttgtatt tatcccaagc tggcaccaca gccgcttaat gaagattctt tattgagtgg
    10081 ttttctcgaa gaaaccaatg agccttatgc cattgctaaa atagcaggca ttaaactttg
    10141 cgaatcctac aaacgacaat acgggtgtaa ttttatttct gcaatgccaa cgaatttata
    10201 tggcccgaac gacaattatg atttaaataa atcgcatgta ttaccggctt taatccggaa
    10261 atttcatgaa gctaaaatga ataatttgcc ggaggttgtg atgtggggaa caggctcgcc
    10321 atatcgtgag tttttgcacg cggatgattt ggcagatgct ttgtttttct taatggaaaa
    10381 ttacgaaggc gaacaatttg taaactgtgg aagcggggaa gaaattacga ttaaagactt
    10441 ggctttattg gtaaaagata ttgttgggta tgatggaaat attgttcatg atacgaccaa
    10501 acctgatggt acgccacgca aactgaccga tgtaagcaaa ttacatgcaa tgggttggaa
    10561 acataaaatc agcctgcagg atggcgtgcg aaaagtgtat gaagatttcg cgcgaaatta
    10621 cgagtctatc atccaaaaat atgcaaacta aaatccgtgc cggtatagta ggtgcatccg
    10681 gttatacagg cggtgaatta attcgtttgt tagtacaaca tccaaatgca gaaattaatt
    10741 ttgcctacag tcgcacacat catgggaaag ccctcgccac tattcattct gatttaattg
    10801 gtgatactac tttacaattt accgattcca ttaatcagga cgtagatgtc gtttttactt
    10861 gcctgccgca tggcgaaacg gggacgtttt taaaaaataa tcccatttct gataaagtaa
    10921 aaatcattga tttaagtaat gaattccgct taaaaaatac tgcttccggc tttgtttatg
    10981 gattaccgga aatcaataaa aacaagataa aatcagctca gaagattgcc aatcccggat
    11041 gttttgccac cgctatacaa tcagcactgt tgccattagc caaggcgggt ttattgcagt
    11101 cggctatcca tgtttccggt acgacaggaa gtacgggtgc cggaaataaa ctcactgctt
    11161 catctcattt tacctggcga aacaataatt tttcaactta taaggcattt acccaccagc
    11221 atctggatga aatcggagag agtgttttgc agttgcagcc cagctttagt tcggctataa
    11281 attttattcc acatcgagga aattttaccc gtggaatttt gataacggca tatacagaaa
    11341 ttgaaagttc tttagacgaa attaaaaaga tttataatgc attttattcc gatgcaccgt
    11401 tcgtctttat ttcagatgta aatatcgata tcaagcaagt agtgaatacc aacaaatgcc
    11461 tattgtattt ggaaaaacaa ggaaaccaat tgctggtaac cgctgtactg gataatctgc
    11521 ttaaaggtgc atcggggcaa gccgtgcaaa atatgaatct gatgtttggt ttcgatgaga
    11581 tggccggact tcggctcaaa cctttggcat attagcaata aatgtcattt gtgtgagcat
    11641 aggtgtgcca cgaacatctt ggcacgccat cactttcaaa aataataaaa ctgtcttaat
    11701 gcattaataa ctccctaaat ctttggtaaa tatcaaatta gcgtactttc atcctttgta
    11761 taatggagac aatacacacg gatgttgtaa ttatcggcgc tggatttaca ggtttggcct
    11821 gtgcatatta tttacaacaa aaaggctatt cagttaaggt tgtggaggtg tcttccagta
    11881 gcggtggcag agccagaacg gataatgtaa atggcttttt gttagacaga ggaactcatt
    11941 tttatcacca ttctactact gaactaaaga aaataatcag cattgataaa ttgggattaa
    12001 aaaatctcta tcccggatat ttactgcgct atgagaatgg atttcatttg tttaccaatc
    12061 cgctttacaa aactttcgac accatttcaa cggtgtttgc tcccaatgca actgtagcag
    12121 acaagatgag gctttttggt ttgtatgtca aactgaaaac attgccttat tctactttgg
    12181 taaaagaaaa tgaatcgtcg acgtatgagt atctttcaaa aaatggattt tcttccaaat
    12241 taattgatac gctctttcgt ccgctggtgg cagccaatat tttcgattat aatttgcagt
    12301 catcctctcg gttttcaaag gtatatctca aatccttatt tcaagagcac gtggcattgc
    12361 ccaaagaagg aatcggttca gttgcaaaac atattttgga taaactcaaa ccggacacgg
    12421 tcatgtttaa caccaaagct aaaagaatac accaagacgg tattacatgc agtaatggaa
    12481 aaacatcacg catagtgtca aaatttacag taattgccac caacccgata gataccaatt
    12541 tactcatgca aagtgatgtc ttgcctacgg agtgttcgca tgtttctact ttgtattttt
    12601 attcagagaa cccaccactg tctaaacctg ttgtggtgct gaatggtaac agcaatggta
    12661 tggtcaatca tatttttgtg ccgacaaacg tgcacccgaa aatggcaccg gaaggaaagc
    12721 atcttattgc tgtaagtgtt gtgaaaacaa atgacttgga tgatgaagaa ttgccggaag
    12781 ccgtaatgaa agaattgaca gaatggtttg gtcttaaggt gatggactgg acgcacctta
    12841 aaacctatca tataaaatac gccatgccct ttaagcccat tttagatgag gtaacttact
    12901 acaaaaaaat aaacgacacg gtttatgctt gcggtgatag ccttagtgtg ggcagtatgg
    12961 aagccgcctt gcgcagtggc agagaaacgg cacaagcttt aataaaagaa ttgaaatcaa
    13021 ttcctgctta aatcacggaa ataatcgttt tgaaaaatcg ccggacttag gtatcggcaa
    13081 taataccgaa gcagataaaa ttgcaagcaa tataagtggc aataccgcta gattatattt
    13141 ttgaggattg agccaccaca gcaaaattag gaatatcagc aaaactcggt atgcttttgt
    13201 gaaaatgata attgcgttag gtactgattt tttcagatta aaaacaataa atggcaaaag
    13261 taatggcatc gcccatataa tattcagatt gtatttagtc gttttatggt cggtgaaaaa
    13321 ccacataaag ataaacagcc aaccaaaaag acccagtatg cataaataga ttaccggtat
    13381 aattttatag cggtataaaa gttgataata gatttgtaaa agaaacaaca ctaaaaataa
    13441 aaaccagaat acaagtgatg gagtaagcca gtttttgttc atgtgttcgg gtacagtgtc
    13501 taaaataagc cggttggaca gggttagttt tttgccgtct gctgttgcac catcaaaaga
    13561 ttgcattaat tcatcaggca aaaaggtacg cccaaattcg gcttttttat ccgtacgtgc
    13621 cccaatcagt aaatccatac caaaatccag ccaggaatca ttcgcatatt ttctgattag
    13681 ttttcgatag gaaacgtcat catctgcatc tttctgaaac tggacggtat tgaaaagcga
    13741 taagatctta tctctcggac gcgtagagca attatcaaat aagaaatcat acttgtagta
    13801 tttgttttct tcgcgagcat taaaggtaag tgcttcaaat agtttttgtt tttgaatagt
    13861 atccagattg agtatttgct cgcttacgct tctgttttct tcgatatatt cagcaataaa
    13921 atcgggatac atgtcaatcg tgagataata caagagcctg cctttcacaa aattccagat
    13981 aaattcggat tcagaattac caaagctaaa cgtgccgtaa ttgtacacaa tatcccaacc
    14041 tttgatacta tccttaatgc gtatagccgt atgtccgaat ttctgccaca cttccgcatc
    14101 gcttcgtcct acagtcagca aacttatttt ggcataaggc gaaagcttcg ccactttaaa
    14161 cgaatcattt acaccaaata cacttgatac ggagcaagaa aaataaagga ggaataagag
    14221 cctatacgtc gatattttca tacagcgaat ataaacaatt caaaaaaaat gctaccttaa
    14281 tccacattat gatacggtac tttttttgga taagctgtgt ttttattttt acggggtgta
    14341 aaacatcaca taaaacagaa tcaaataata aagcagtaac
//
LOCUS       DAOQII010000034        14192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2069_length_14192_cov_5.000778, whole genome shotgun sequence.
ACCESSION   DAOQII010000034 DAOQII010000000
VERSION     DAOQII010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2069_length_14192_cov_5.000778"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1426)
                     /locus_tag="PLF48_02895"
     CDS             complement(71..1426)
                     /locus_tag="PLF48_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48301.1"
                     /translation="MKNTKVYTWLKNCSDYHLAQLQMLLNTPETKRELVNLYLGFVRH
                     LHEDYPKEKLYTEVFKEKKFNGNKFRKACHELLKLAEQKIVCLYLDEHPNLQDNLRLQ
                     FYSSNGWTEHYADLFAEIKLTEQKHFERFSFAYLDYMHGIINPQKSNSLKIKIYSDSL
                     DHFYLLEKMKLICHAQNESHFTKFSYQPDFTAVLLPIIQNKIHTDALLHCYYLSYLML
                     LHTDKEHAYFEIKQQLAQTQIPLADKKIIFQYLINFCIIRINKSEQQFERELFEVFKL
                     YYSIITENEISPFRFKNVINLSLKLKEYTYAEYFNTTYGELLDISIRESTVIFNQAKI
                     AFEMKQYDTTLMFLHKARFDDLTFNLSSRILITKTFYEQKEMQFLENYLESFRIYILR
                     NKAMNSGNKKLHQDFIKLIRQLMRKEYAGKKQTEAFKQKINAVPNLPDKFWILEKVEE
                     L"
     gene            1592..4063
                     /locus_tag="PLF48_02900"
     CDS             1592..4063
                     /locus_tag="PLF48_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48302.1"
                     /translation="MKNISLISKIRWNASKATLVLLFLVAFNYSGNAQNCNFSNSDFD
                     ISTIGNHAYLSNINSNWHSYEIIFDSTGRWQYYNNDISFNSPGLKEICMHVVDTSDLN
                     VPCDTFICKTVNIQIDTCPDIIDYFSFSTYQNADTFIANLYFNIDGYTSYQFDYGDGT
                     SGTSNYHNYPDNSPRTVCLMVNNSNFSGCEKTSCITIVFDTINQSACNLQPLEFQKYI
                     DTIIRLNIYDYYSNPSASRIFEDNINIASNSWANNGFKFFVYDTIQETWQTYQIGSTC
                     RGPYNDINCGRAPASVIEFPTTTLEERKHLIRFLYSIPCESYVFGMSLPYPKYGEWLS
                     DSTQVNDTSLFKAFEDLGISKIRQQTDSLPFVFFSQKCNLEFNTEQISRANPRDSITA
                     SFSFHSKYTQRTCRNSEIIRYYSSAVGTVYKIIPLSSSLYSEYYICRDGQIIYCNDAD
                     TTSYCHRYLNNLTYMGNYWSCDSNTTTCDSVTCVLPGDADHDLTVNNFDVLAIGLSYG
                     RLGLRRPDATNSYTLQPSPDWQTTHYYGYNDKFADCNGDSIINAQDAVVIYNNYIERA
                     VNVFNHRDAQLDSLPQVTLTFDTLPIIIANGTCAGAQIAGDININSVTTIDSSYGIAF
                     SVEYPESFYDSCFSVAVDLDNNSWFVSNEPVILFYKNIPQFHRVDVSMVRTNGLNRSG
                     NGRIGRIKFITEGDIFGRQLRNSSSYVYTFNVNSTQAIDNRGNKINITGSSTTVSFIS
                     LGYKQSEIHNLKVFPNPLINILQLRADERISEIQLTDITGKIIYASHPDTYQYQIDCS
                     ELPSGIYIMQIKSDEGLSVIKLVRQ"
     gene            4247..4630
                     /locus_tag="PLF48_02905"
     CDS             4247..4630
                     /locus_tag="PLF48_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48303.1"
                     /translation="MKQTTKKIAKALIAFMLFSPLFIACVPNGGGPNPTPHCYRDSFA
                     VHYDSLNHTWDTTIIRVPASCNDTTPIPPVICDSVPFDSLLITPVYSWICDSTNTNPN
                     DTLHCHYIISEYDTTMHTFIRINCH"
     gene            4723..6207
                     /locus_tag="PLF48_02910"
     CDS             4723..6207
                     /locus_tag="PLF48_02910"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNF48304.1"
                     /translation="MKKTVLFLLFIVNLLISQFSFAQLDRYCGMDVINNNIIQSDAAI
                     KSTYLEQINQAVNDIMAYDTSLARTDSVYTVQVVVHVVYLNNNKYENIPDDIIRTQID
                     ALNRDYNALNADSVNLRPFFVPFRGNPKIKFQLATKTPGGQATTGITHTQGALGNLAG
                     WDPISTLIWTALGIEPLKVDNLILTGSKGKSAWNVNKYLNIWVCDLNYGNRKCRTCLT
                     LCDTCGALGGFAYPPSNATNWGGLALNHGNNDGLVIDFRFFGINNWFARDSTSQRFRT
                     LYTNGRTTVHEVGHYFGLQHTWGNVVELPGGPSVDGCTIDDFMADTPEEEQAFAANLP
                     RGTTNVCDTTINTCNKLYLGRDYPDMFENYMDYSSDLCYNLFTKQQSEIMRFNLLSRR
                     SGLIIKREGSTVLATNQIRTAEAGIRFYPNPTSSQLTVEFSKPSKQAIQIAVYDITGK
                     KMLAQTASINETVISLSTEKLAKGIYLLQFSNDEFIAADKFIKE"
     gene            complement(6291..8834)
                     /gene="topA"
                     /locus_tag="PLF48_02915"
     CDS             complement(6291..8834)
                     /gene="topA"
                     /locus_tag="PLF48_02915"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNF48305.1"
                     /translation="MTKNLLIVESPAKAKTIEKILGKDFTVKSCYGHIRDLAKKDMGI
                     DIQNDFKPNYIISEDKEQVVKELQKLSKNAEVWLATDEDREGEAISWHLCEVLDLDKH
                     NTKRIVFNEITAHAIKAAVANPRQLDHNLFYAQQARRVLDRVVGFELSPVLWKKLGRR
                     TLSAGRVQSVAVRLIVEREREINNFKSDSFYKVVAYFISDKKSFKAELSEKFKAEEEA
                     LDFLEAAKNAIFKIEDIQVKPAQKSPAAPFTTSTLQQEASRKLGFSVSRTMSVAQKLY
                     EAGKITYMRTDSPNLSELALNTIQSYINQEFGKEYSNRKQYSAKSSNAQEAHEAIRPT
                     YIDHTHVSGHRDEERLYELIWKRTIASQMSNAKLEKTIAKIDISNRKEKFNAEGEVLK
                     FEGFLKVYLESSDDDEQEETGMLPPLQVGQLLDYKNIEARQRFTQAPARYTEASLIKK
                     LEELGIGRPSTYAPTISTIQNREYVVKGDREGLPRSFQRLELTNKGEIKSETLSENTG
                     SLKGKLLPTDIGTLVTDYLMENFNRVMDYSFTADMEKDFDLIAEGNKEWTKVLKQFYG
                     PFHKEIETSEESTEYVTGERQLGIDPETKRQVSARLGRFGPMIQLGTAEEDEKPRYAR
                     LKPSQSLETITLEEALELFKLPLILGTYEGAEVAVNIGRFGPYVKHGEQFISLPRGEE
                     PADVTLERAIEVIKQKLVEDAPIAMYEGKPVTKGKGRFGPFIKWNDMFINVPRAYNFD
                     RLTQNDCDELIEKKLDKEANRYILQFPEEKISVENGRWGPFLRFGKKMLKLTGKKYTQ
                     DEAAMLTLDELKKMVEAQVPDAFAKKEKKAPAKKAAAKKSATKKPTKKK"
     gene            complement(9136..10911)
                     /locus_tag="PLF48_02920"
     CDS             complement(9136..10911)
                     /locus_tag="PLF48_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF48306.1"
                     /translation="MSELLLDVKNLVTEFNTENGLVKAVDNVSFHINKREVVGIVGES
                     GSGKSVTSLSIMRLIPNPPGKITNGEILYCRDNEKPVDLVHISEQEMRSFRGNDISMI
                     FQEPMTSLNPVFTCGNQVMEAIMLHQKVSKAEAEQKTLALFEQVKLPNPQRILKAYPH
                     ELSGGQKQRVMIAMAMSCQPKILIADEPTTALDVTVQKTILELIRDLSDQTGMSTIFI
                     THDLGVIAEIADRVIVMYKGKIVEQGTVLDIFSNPQHPYTKGLLACRPPLGKRLSRLP
                     VISDFMEEKNGVVTEKESDMQKIVDAYVVSDVQTEKRMENLMQTKPILELKNIQTWFP
                     SKTNLFGKPLEYVKAVDDVTFDVYEGETLGLVGESGCGKTTLGRTILRLVEPTGGKAY
                     FDGKDIFALEQKELKELREHMQIIFQDPYSSLNPRMTIGNAIMEPMQVHNKYSNDAER
                     KDKVIELLEKVNMKPEHFMRYPHEFSGGQRQRICIARALALNPKFIICDESVSALDVS
                     VQAQVLNLLIQLREEFKFTYIFISHDLSVVKFMSDRMVVMNKGKIEEMGAADEIYNNP
                     QKEYTKKLIAAIPKGNVEDIRARFV"
     gene            complement(11001..11726)
                     /locus_tag="PLF48_02925"
     CDS             complement(11001..11726)
                     /locus_tag="PLF48_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNF48307.1"
                     /translation="MLNTVRLTDLIYIDIETAPVCKTFEDLTALEKILWNEKKGRLKP
                     ENESEEEYYFQHAGIFAEFGKLICISVGWFMEDKDFSGLKIKSFYGHDEIQLLKDFAL
                     FLQHAVASNKNKQLCGHNIKEFDIPFICRRMLMNNFPLPDYFEQLQAKKPWEAGLLDT
                     MDLWKFGDYKNYISLKLLAYSLGIPSPKDDISGSDVGKVYWNEQDIERIKTYCQKDVL
                     TVAQIILKLKGKKLISENEVLFV"
     gene            11815..12774
                     /locus_tag="PLF48_02930"
     CDS             11815..12774
                     /locus_tag="PLF48_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48308.1"
                     /translation="MSENNSNSKQHYIYIALIALLIGGLIYTQLKLKQSKDENKRTEQ
                     EKGQLESLRSELDKKYQDAMLEIENYRAENAGLDSLLNVKEHELSEKRSRIESLLAQV
                     ATIKQSGKEDKTKMQQLLNEANALIKELEADKARTQNSIDSLLAANKQLYMERDSFSS
                     ELSTTKVKKQASDDENKLMKERIDRASILSTANLQCNAVHTKRNGKEDETSKAKDAEK
                     LKICFDLLQNKIAPKGETEIMVRVITPTGSTIQLQTLGSGTFTNDEGKSIPFTYSIKP
                     DYQNESKTVCSYWQQDMNFSSGKYSVEVYQKGILIGQANFMLK"
     gene            12813..13724
                     /locus_tag="PLF48_02935"
     CDS             12813..13724
                     /locus_tag="PLF48_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF48309.1"
                     /translation="MKSNYYKGILLVFIGAFCFSTKGIFAKLAFRYGVTGLDILTLRM
                     LFSLPFYIAIYFIQQKKHPVKISKDTFLQILFLGIIGYYLAALFDFLGLQFVSANLER
                     VIIFTYPTFVLLLGFIFYKRNISRIQISAILLSYAGILIAFISDYKTASGSSIFLGVL
                     YVLLSALTYAFYLVRSDKLILQIGTAMFTCISMIISCLAVLIHFGIVNHFSIFNYPLP
                     VYLIGVCIAIISTVLPSFLMTAGIKYIGSSNMSIVASIGPVSTIFLSFYVLNEDVTFL
                     HITGTVFVLAGILALTLFGGKSTKNIT"
     gene            13952..>14192
                     /locus_tag="PLF48_02940"
     CDS             13952..>14192
                     /locus_tag="PLF48_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48310.1"
                     /translation="MKKLLIAIVVFALLFAISPFFIKQGLIHNFSNVDDYKIFSNHEI
                     KKSVAPQPWLAASDYNKKKLSKEMTDFFDERKTCAF"
BASE COUNT         4429 a         2702 c         2359 g         4702 t
ORIGIN      
        1 cctatagtcc aaacccatac ccaatactca ctacccacta cccactaccc acatctcggc
       61 tctatctcct ttacaattct tccactttct ccaaaatcca gaatttatcg ggcagattgg
      121 gaacggcgtt tattttttgt ttaaaagctt ctgtttgttt ttttcctgca tattctttac
      181 gcatcaattg tcggatgagt tttatgaaat cctgatgtaa ctttttatta cccgagttca
      241 ttgctttatt gcgcaaaata taaatcctga aactctccag ataattttca agaaactgca
      301 tttctttctg ttcgtaaaat gttttagtaa tcagaattct ggaacttaaa ttaaaggtca
      361 aatcatcaaa acgggcttta tgcaagaaca ttaaagtcgt atcatattgt ttcatctcga
      421 aagcaatttt agcctgatta aaaatgactg tcgattctct gattgaaata tccaacaatt
      481 cgccgtacgt ggtgttaaag tattcagcat acgtgtattc tttgagtttc agcgacaggt
      541 tgattacatt tttgaaccgg aatggagaga tttcattttc tgtaattatt gaataataca
      601 acttaaaaac ctcaaacaat tcgcgttcaa attgctgttc acttttattt atccggatga
      661 tgcagaaatt gattaagtat tgaaaaatta ttttcttatc ggcaagggga atttgcgttt
      721 gcgccaattg ctgtttgatt tcgaaatatg catgttcctt atctgtatgc agcaacatca
      781 gataagacag ataatagcaa tggagcaatg catccgtatg gattttattt tgaatgattg
      841 gtaacaacac agcagtaaaa tcaggctgat atgaaaattt tgtaaaatga ctttcattct
      901 gagcatgaca aattaatttc attttctcca agagataaaa atgatccagc gaatcagaat
      961 agatttttat tttaagtgaa ttcgattttt gcggattaat aatgccatgc atataatcaa
     1021 gataggcaaa actgaaacgt tcaaaatgtt tttgttcagt aagtttaatt tcagcaaata
     1081 aatcagcata atgttccgtc cagccattac tgctgtaaaa ttgcaaacgc aaattatcct
     1141 gcaagttcgg atgctcatca agataaagac atacaatttt ttgttctgct aatttcagca
     1201 attcatggca ggctttgcga aatttgttgc cgttgaattt cttttctttg aaaacttccg
     1261 tatataattt ttctttagga tagtcttcat gcaaatgcct gacaaaaccc aaatacaaat
     1321 tgactaattc tcgcttcgtt tccggagtat tgagtagcat ctgcaattgg gctaagtgat
     1381 aatcagaaca atttttcagc caagtataaa ccttagtatt tttcattaga ataaaattgc
     1441 ataaaaatat gatttagtat tttgatttac aactaattag taaaataata aagaataaaa
     1501 catggaataa ttatcaaaat ttgtactatt ttctaaacaa aagaatattc aaatttgtat
     1561 cgtaaagaat tgtacattta aaattagttt tatgaaaaac atatccttaa ttagtaaaat
     1621 tagatggaac gcatcaaaag cgactttagt actcttgttt ttagttgcat ttaattattc
     1681 aggaaatgca caaaattgca atttcagcaa ttccgatttt gacatatcga ctataggaaa
     1741 tcacgcctat ttaagtaata tcaattcaaa ttggcacagc tatgaaatta tatttgacag
     1801 cacgggaaga tggcaatatt ataataatga tatttctttt aattctccgg gactaaaaga
     1861 aatttgcatg catgttgtcg atacttccga tttgaatgtg ccttgtgata cctttatttg
     1921 caaaacggtt aatatccaga tagacacttg tccagatatt attgactatt tttcattttc
     1981 aacttatcaa aatgcagata cattcatagc taatctttat tttaatattg atggatatac
     2041 ttcgtatcaa tttgattatg gagatggaac atctggaaca tcaaattatc ataattatcc
     2101 ggataatagt ccgcgaacag tttgcctgat ggtaaacaat tctaatttta gcgggtgtga
     2161 aaaaacgagt tgtattacaa tagttttcga cacaataaac caatcagctt gtaatttaca
     2221 gccattagaa tttcagaaat acattgatac gattattaga ttgaatatat atgactatta
     2281 tagtaaccca tctgccagtc gaatttttga agataatatc aatatagcat caaactcatg
     2341 ggcaaacaat ggttttaaat tctttgtata tgatactatc caggaaactt ggcaaactta
     2401 tcaaattgga agtacttgta gggggccata caatgatata aattgtggca gagctccggc
     2461 ttcagttata gaatttccca ctacaacatt agaggaacga aaacatctta tccgattttt
     2521 atattcgatt ccttgtgaaa gttatgtttt tggaatgagc ttgccttatc caaaatatgg
     2581 tgaatggcta tcggatagca cacaagttaa tgacacttca ctttttaagg catttgaaga
     2641 tttaggaata tcaaagatac gtcaacaaac agacagttta ccttttgtgt tcttttcaca
     2701 aaaatgtaat ttggaattta atactgaaca aatttcaaga gcaaatccaa gagattcaat
     2761 tactgcttca tttagttttc actctaaata tacccaaaga acctgtagaa atagtgaaat
     2821 aatacgatat tactcatctg ctgtcggtac tgtttataaa ataattccat tatcgtcttc
     2881 attatatagt gaatattata tttgcagaga tgggcaaatt atatactgca acgacgctga
     2941 tacaaccagt tattgtcatc gatatttaaa caatcttact tacatgggta attattggtc
     3001 atgtgatagt aacacaacta catgcgattc tgtcacctgc gttcttcccg gtgatgccga
     3061 ccatgattta acggtgaata atttcgacgt gttagctatt ggtttaagtt atggaaggct
     3121 cggtttacgc cgacctgatg ctacaaattc ttacacgctc cagccttcac ctgattggca
     3181 aacgacacac tactatggtt acaatgataa atttgccgac tgcaacggag acagcatcat
     3241 taatgcgcag gatgcggttg taatttataa taattacatc gaacgagcag tgaatgtatt
     3301 taatcaccgt gatgcacagc tggattcgct gccacaggtt actttaacat tcgatacctt
     3361 gcctattatt attgcaaatg gcacttgtgc cggtgcgcaa attgccggag atatcaatat
     3421 caattccgtt actacgattg acagttctta tggaatagca ttttctgtag aatatcctga
     3481 atcattttat gattcttgtt tttcagttgc tgttgattta gataataact cctggtttgt
     3541 gagcaacgaa cctgttattt tattctataa aaatattcct cagtttcatc gggtagatgt
     3601 gagcatggta cgtactaacg gtttaaacag aagtggtaac gggcgaattg gcagaatcaa
     3661 atttattacg gaaggcgata ttttcggacg tcaattgcga aactcttcat cgtatgtcta
     3721 tacgtttaat gttaactcga cacaggctat tgataaccga ggcaacaaga taaacattac
     3781 cggttcttca acaacagtca gctttatatc attgggttat aaacaaagtg aaatacacaa
     3841 cctgaaggta tttccaaatc ctttaattaa tattttgcaa ctcagagctg acgaacgcat
     3901 ttcagaaata cagctaacag acatcaccgg aaaaattata tatgcatcac atccggatac
     3961 ttatcaatat cagattgatt gctcggagtt gccatccggt atttatatca tgcaaattaa
     4021 atcagacgaa ggattatctg ttattaagtt agtcagacaa tagaagttag tagtaagtat
     4081 ttagtaagaa gtagtaagta gtaagtaaaa agatgcttat atgccattct aatatttaaa
     4141 attcaccatt catcattcac cattcatctc tcatcattca tcactcatca ttaatcattc
     4201 accattaaaa aatatccttt ttattaactc atttaaattt aaaacaatga aacaaacaac
     4261 aaaaaaaatc gcaaaagcat tgatcgcatt catgctgttt tcacctctgt tcatcgcatg
     4321 cgtacctaac ggaggcggac caaaccccac accacattgc tacagagatt cgtttgcagt
     4381 acattatgac agtctgaatc atacttggga tacgacgata atcagggttc cggcttcgtg
     4441 taacgatacg acaccaatac ctccggttat ttgcgattct gttccttttg attccctttt
     4501 gattactcct gtgtattcat ggatttgcga ttcaactaat acaaatccga atgacacttt
     4561 gcattgccat tatattattt ccgaatatga cacaacaatg catacattta ttcgaattaa
     4621 ctgccattaa tcagaaacat ttagattttt agacatgccg gatatatttg tccggctttt
     4681 ttttatgctt tacaatcagt atttttgcag taaatcgttt atatgaaaaa aacagtactc
     4741 tttttgttat ttattgtcaa tctgttaata tcgcaatttt cttttgctca actagacaga
     4801 tattgtggaa tggatgttat taacaataat attatccagt cagatgccgc cataaaatca
     4861 acctatctgg agcaaatcaa ccaagcagtc aatgatatta tggcatacga tacttcgctg
     4921 gcaagaacag atagtgttta cacggtacaa gtcgttgtac atgtagttta tctaaacaac
     4981 aacaaatatg aaaatatccc tgatgatatt atacgcacac aaattgatgc acttaacaga
     5041 gattacaatg cactgaatgc agacagtgtg aatttacgcc cgttcttcgt accattcaga
     5101 ggcaatccaa aaattaaatt tcaactagct actaaaacac ccggtgggca agctaccaca
     5161 ggcattacgc atacacaagg tgctttggga aatctggctg gttgggatcc aatcagtaca
     5221 ttaatctgga cagccttagg tatagagcca ctaaaagtag ataatttaat tttaacgggg
     5281 tctaaaggaa aatccgcttg gaatgtcaat aaatacctga atatctgggt atgcgatttg
     5341 aattatggaa acagaaaatg tagaacatgt ttaactttat gtgatacgtg cggtgcatta
     5401 ggtggctttg cataccctcc ttcaaacgct acaaactggg gcggactagc attaaaccat
     5461 ggcaataacg acggattagt gatagatttt cgattttttg gaattaacaa ctggttcgca
     5521 cgcgattcta cttctcaacg tttcagaact ttatatacta atggcagaac tactgttcat
     5581 gaagttggtc actactttgg cttacaacat acttggggaa atgtagtaga acttccgggt
     5641 ggtccaagtg ttgacggttg tactattgat gattttatgg cagatacacc tgaagaagaa
     5701 caagcatttg cagcgaattt accaagagga acaacaaatg tttgtgatac cactattaat
     5761 acttgtaata aactatattt agggagagat tatccggata tgtttgaaaa ttacatggat
     5821 tattcatcag atttgtgtta taatctgttt accaaacaac aatctgaaat catgcgcttt
     5881 aatcttttgt caagaaggag cggtttgatt attaaaagag aaggttcaac cgttttagcg
     5941 accaatcaaa ttcgtactgc tgaagccggt atcagatttt atcctaatcc tacaagttct
     6001 cagctgacag ttgagttcag caaaccttca aaacaggcta tacaaattgc ggtatatgat
     6061 atcacgggca aaaaaatgct tgctcaaaca gcatcaatta atgaaactgt catttcttta
     6121 tcaacggaaa aactggctaa gggtatttat ctgcttcagt tcagcaatga tgaatttatc
     6181 gcagcagata agtttataaa agaataatcc tatctaatag aaatttaaaa aaggctgcga
     6241 ttttacgtag cctttttaag atttctgaaa ttcagattaa ctgaactatt ttatttcttt
     6301 ttggtaggct tcttagttgc tgattttttc gctgccgctt ttttagccgg tgctttcttt
     6361 tcctttttgg caaatgcatc cggtacttga gcttcaacca tttttttaag ctcgtccagt
     6421 gtcagcatgg ctgcttcatc ctgtgtatat tttttcccgg taagtttcaa cattttcttg
     6481 ccaaagcgaa gaaacggccc ccacctgccg ttttcaacag aaattttttc ttccgggaat
     6541 tggagtatat aacgatttgc ttctttatcc agcttttttt ctatcaattc atcgcagtca
     6601 ttttgtgtaa gtctgtcaaa attataggct ctcggcacat tgataaacat atcattccat
     6661 ttgataaaag ggccaaaacg acctttacct tttgttaccg gtttaccttc atacatggca
     6721 attggtgcat cttccactag cttttgttta atcacctcta ttgctctttc taaggtaaca
     6781 tccgcaggtt cttcgccacg cggcaatgaa ataaactgtt ccccatgttt tacatatgga
     6841 ccaaaacgtc caatatttac ggctacttca gctccttcat aagttcctaa aatcaaaggc
     6901 aatttaaaca attctaatgc ctcctccagt gttatagttt ccaaactttg agaaggtttt
     6961 aaacgggcat aacgaggttt ttcatcttct tctgcagtac ctaattgtat cataggccca
     7021 aatcttccca aacgcgcaga cacttgtctt ttggtttcag ggtcaattcc taattgtctt
     7081 tcacctgtta catattccgt actctcttct gaagtttcta tttccttatg aaaaggaccg
     7141 taaaactgct tcaaaacctt agtccactct ttattaccct ctgctattaa gtcaaagtct
     7201 ttttccatat ccgcagtaaa agaataatcc atcacacggt taaaattctc catcaaataa
     7261 tctgtaacta atgtaccgat atctgtaggt aataattttc cttttaaaga gcctgtattt
     7321 tcagataatg tttctgactt tatttcacct ttattagtga gctctaatcg ttgaaaagaa
     7381 cgaggtaatc cttccctatc gccttttacc acatattctc ggttttgtat cgtagagata
     7441 gtaggagcgt aagtagaagg acgaccaata cccaactctt ctaacttttt aatcaaactg
     7501 gcctctgtgt atctggcagg tgcttgcgta aaacgttggc gagcttcaat gtttttataa
     7561 tccaagagct gccctacctg caaaggcggc aacataccgg tttcttcttg ctcatcatca
     7621 tcactgcttt ccagataaac ttttaaaaag ccttcaaatt ttagcacttc accttctgca
     7681 ttaaattttt cttttctgtt ggaaatatca attttggcaa tggttttttc cagtttggca
     7741 ttactcattt gcgaggcaat tgtacgcttc caaattagct catataagcg ttcttcatcc
     7801 cgatgaccac tcacatgtgt atgatcgata taagtggggc gaatggcttc gtgcgcctct
     7861 tgtgcattag aacttttagc actgtattgt tttcgattgg agtattcctt tccaaactcc
     7921 tgattgatat atgactgtat cgtgtttaac gccaactcag ataaattggg tgagtcggta
     7981 cgcatataag taatcttacc ggcttcgtac aatttttgag caactgacat ggtgcgtgaa
     8041 actgaaaaac ccaacttgcg tgacgcctcc tgttgtaagg tagatgtagt aaaaggtgca
     8101 gccggacttt tttgcgccgg ctttacctga atatcttcta ttttaaaaat ggcatttttt
     8161 gctgcctcca aaaaatccaa agcctcttct tcagctttga atttttctga aagttctgct
     8221 ttaaacgatt ttttatctga gataaaatag gcgacaactt tatagaaact gtctgatttg
     8281 aaattattga tttcacgttc gcgctctacg attaagcgaa cagccactga ttgcactctt
     8341 ccggcggaaa gcgttcttct gcccagcttt ttccatagca ccggcgataa ttcaaaacca
     8401 acgaccctgt ctaaaacgcg acgagcctgt tgagcataaa ataaattatg atccaattgg
     8461 cgaggattag caactgcagc cttaattgca tgggctgtaa tctcgttaaa tacgatacgc
     8521 ttggtattat gtttatctaa gtccagcact tcgcacaaat gccaggatat ggcttcaccc
     8581 tcacggtctt catccgttgc cagccacact tcagcatttt tagacaactt ctgcaattct
     8641 tttacgactt gttctttatc ttctgatatg atgtaattgg gtttaaaatc gttttggata
     8701 tcaataccca tatctttttt agccaaatcg cggatatgcc catagcatga tttcacggta
     8761 aaatctttac cgagaatttt ttcgattgtt tttgccttag ccggcgactc cactatcaat
     8821 aaattttttg tcatactata aacttttagc acaaatgcaa tacaccgcaa atgaaataaa
     8881 aaaaataacg gcacaaataa aaaataaatt ttcgataata tgcagtaaaa tgagcggaaa
     8941 aattgggatt agcaaattcg ttaatttaag aaattgcaga tttgatggtg taaatgttat
     9001 aaattcaaga attggtacag cgtattagtg tgtattcaga tttcaattaa aataaatatg
     9061 cagaaataac aagctcccaa atagtgctat tatactaaga tattgtatta tgcattaaac
     9121 taaaacaatc aactactaca caaaccttgc tcttatatct tcaacattgc ctttgggtat
     9181 ggcggcaatc agtttctttg tatactcttt ttgtgggttg ttataaattt catcagcagc
     9241 gcccatttct tcgattttac ctttgttcat caccaccatt ctgtcactca tgaatttaac
     9301 aacggataaa tcatgtgaaa taaaaatata ggtaaattta aattcttctc tcaactgaat
     9361 caataaattc aatacttgtg cttgtacaga cacatccaat gccgaaactg attcatcgca
     9421 gatgataaat ttcgggttga gtgccagagc tcttgcgata caaatacgct ggcgttgccc
     9481 gcctgaaaac tcatgcggat agcgcataaa atgctccggt ttcatattta ctttttccag
     9541 caattctatt actttatctt ttcgttctgc atcgttgcta tatttattat gcacttgcat
     9601 gggttccatt atggcattgc caatcgtcat ccttggatta agagaggaat atggatcctg
     9661 aaaaatgatt tgcatgtgtt cgcgcaattc ctttagttct ttttgctcta atgcaaaaat
     9721 atccttaccg tcaaaatatg cttttcctcc ggtcggttca accagtcgta aaatagtacg
     9781 ccctaaggtg gttttaccgc atccgctctc tcctaccaaa cccagtgttt caccttcata
     9841 cacatcaaac gttacatcat ctaccgcctt tacatattcc agaggcttgc cgaataaatt
     9901 tgttttgctc ggaaaccagg tctgaatatt tttcaattcc agtatcggtt ttgtttgcat
     9961 cagattttcc attcgttttt ctgtctgcac atcacttacc acataggcat ccactatctt
    10021 ttgcatatcg ctttcctttt ccgtaaccac gccattcttt tcctccataa aatcagaaat
    10081 taccggcagt ctgcttaggc gttttcctaa tggcgggcgg caagccagca agcctttggt
    10141 atatggatgc tgcggattgg aaaatatatc caacactgtt ccttgttcca caattttgcc
    10201 tttatacatc acaattacac ggtcggcaat ttctgctatt acgcctaaat cgtgagtaat
    10261 aaaaatagtg gacattcctg tttgatcact caaatcgcga ataagctcta ggatggtttt
    10321 ttgtacggta acatcaagtg cggtagtcgg ttcatcagca atcaaaattt taggctggca
    10381 actcatcgcc atggcaatca tcacccgttg tttttgccct cccgaaagct catgtggata
    10441 ggctttcagt atgcgctgtg gatttggtaa tttcacttgt tcaaacaagg ctaacgtctt
    10501 ttgttcggct tcagctttgc tcactttctg atgcagcatg atggcttcca tcacctgatt
    10561 gccacaggta aaaacaggat tgagagaagt catgggttcc tgaaaaatca tggaaatatc
    10621 attacctctg aatgagcgca tttcctgctc actgatatgt accaaatcaa ccggtttttc
    10681 attatcgcga cagtacaaaa tttcgccatt cgttattttt cccggaggat ttggtataag
    10741 acgcataata gataatgacg taacagattt acctgagccg gactctccta ctataccaac
    10801 tacttcacgc ttattaatat gaaaggaaac attgtcaacg gcttttacca atccgttttc
    10861 ggtattaaat tccgttacca ggttttttac atctaacagt aattctgaca tattgagtat
    10921 tgagtgatga atgattaatt ttgaagtttg aatgtatgag tattgctatt taactatccg
    10981 acatttaatt ctaacgtgca ttacacaaac aaaacttcat tttccgaaat tagttttttt
    11041 cctttcaatt tcaaaatgat ttgtgccacc gttaatacgt ctttttggca ataggttttt
    11101 atgcgttcta tatcttgttc attccagtaa acctttccca catcactgcc agaaatatcg
    11161 tctttaggtg aaggtatccc taagctgtat gccagcagtt tcagactgat atagtttttg
    11221 taatcaccaa atttccataa atccattgta tccagcaaac cggcttccca tggtttttta
    11281 gcttgcaatt gctcaaaata atcgggtaaa ggaaaattat tcatcagcat tctgcggcat
    11341 ataaaaggga tatcaaattc cttgatatta tgtccgcaaa gttgtttgtt cttatttgat
    11401 gcaacagcat gttgtaaaaa caaggcaaaa tctttcaaaa gctgtatttc atcatgtccg
    11461 taaaaagatt ttattttcaa accggaaaaa tctttatctt ccataaacca acccacacta
    11521 atacagatta gtttaccaaa ttctgcaaaa atacctgcat gctgaaaata atattcttct
    11581 tcactttcat tttctggttt aagtcttcct tttttttcgt tccagagtat tttttctaag
    11641 gcagttaaat cctcaaaggt tttacacacc ggagcggttt caatatcgat ataaatcaaa
    11701 tctgtaagcc gtactgtatt taacatatat gtttggtttt ttgggagatt atgcaagata
    11761 cgccatctaa ttcaattaat tcttttaatt ttacacatgt aaaaaaatga agacatgagt
    11821 gaaaacaatt ccaattcaaa acaacattac atatatatcg cactgattgc cttactgata
    11881 ggaggtttga tatatactca gctgaaactg aaacaatcta aagacgaaaa taaacgaact
    11941 gaacaagaaa aaggacagct ggaatcttta cgttctgaac tggataaaaa ataccaggat
    12001 gccatgcttg agattgaaaa ctacagagca gaaaatgcgg gattagacag cttactgaat
    12061 gttaaagaac acgaattgag tgaaaaacgt tctcgtatag aatcattact ggctcaagtg
    12121 gcaacgatta agcaaagtgg caaggaagat aaaactaaaa tgcagcaatt actcaacgag
    12181 gctaacgcat tgataaaaga actggaagct gacaaggcac gaacacaaaa ttcaatagat
    12241 tcgttgctgg ctgccaataa acaattatac atggaacgcg acagcttctc gagtgagctt
    12301 agtacaacta aagttaaaaa acaagcatct gatgatgaaa ataaattaat gaaagaacgc
    12361 atcgaccgtg cttctatact gagtactgct aatttacaat gcaatgccgt acacacaaaa
    12421 cgaaacggga aagaagatga aacctcaaaa gcaaaagatg cagagaaatt aaaaatctgt
    12481 tttgacttat tgcaaaataa aattgcgcct aaaggcgaga cggaaattat ggtacgcgta
    12541 ataactccaa ccggctcaac gatacaattg caaactttgg gaagtggcac ttttaccaat
    12601 gatgaaggca aatccattcc atttacatac agcatcaaac ctgattatca aaatgaatca
    12661 aaaacagttt gttcttattg gcagcaagac atgaactttt catccggaaa atatagtgta
    12721 gaagtttatc aaaaaggcat actcatagga caggctaatt tcatgttaaa gtaatgcagc
    12781 tcctactatc tttgtgatac atgttcctca acatgaaatc taattattac aaaggtattt
    12841 tactcgtatt cataggcgca ttttgcttct ctactaaagg tatatttgca aaactggcat
    12901 ttcgatatgg tgtaacggga ttagatatcc tgacattgcg catgttgttt tcattaccct
    12961 tttatattgc aatttatttt atacagcaaa aaaaacatcc tgtaaaaatt tcaaaagata
    13021 catttttgca gattttgttt ttgggcatta tcggctacta tctggctgcc ttgtttgact
    13081 ttctcggatt acaatttgtg agtgccaatc tggagcgggt aattattttc acttacccca
    13141 ctttcgtact cctactcgga tttatctttt acaagcgcaa tatatcacgc atccagattt
    13201 cagctatctt attgagctat gccggcatac tcattgcttt tatctccgac tataaaacag
    13261 cctcaggcag ttcaattttt ttgggtgtac tctatgtatt gctcagcgca ctcacttatg
    13321 cattttattt agttcgcagc gataaactga ttttacaaat cggcacagcc atgtttacct
    13381 gtatttctat gataatttcc tgtttagccg tactgattca ttttggcatc gtcaatcatt
    13441 tttcaatatt caattaccca ttacctgttt atcttatcgg agtatgcata gctattatct
    13501 caacagtact tccatcattt ttgatgacag ctggcataaa atacataggc tcgtccaaca
    13561 tgtcaatagt cgccagtata ggtcctgtca gtacaatatt tctatcgttt tatgtattaa
    13621 atgaggatgt cacattttta cacatcacag gaactgtttt tgttttagcc ggaatactgg
    13681 cattaacact ttttggaggt aaatcaacta aaaatataac atgaacttta ataatatata
    13741 gttgtattta atttagttgg cgtaaaaata gttgattttt atataacacc ctctactttc
    13801 atcaagaagg aggaacaaaa ggaggtggta ttagaaaata ctaaatcaac tattttggaa
    13861 agttttaatt atatcaagct aactttacta tattgataat atagcggata ttgtcagctt
    13921 attcatcaat ccaatttact aactttaatc aatgaaaaaa ctactgattg ccattgtggt
    13981 gttcgcactg ctttttgcca tttcgccttt ttttatcaaa caaggcttga tacataattt
    14041 ctcaaatgtg gacgattata aaattttctc caaccacgaa attaaaaaat ctgttgcacc
    14101 ccaaccttgg ctcgcagcat ctgattataa taaaaaaaaa ttgtcaaaag aaatgacaga
    14161 tttttttgat gaaagaaaaa cttgtgcatt tc
//
LOCUS       DAOQII010000035        14139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2082_length_14139_cov_5.781383, whole genome shotgun sequence.
ACCESSION   DAOQII010000035 DAOQII010000000
VERSION     DAOQII010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_14139_cov_5.781383"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLF48_02945"
     CDS             <1..511
                     /locus_tag="PLF48_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNF48311.1"
                     /translation="HPPWSALGRPILGAGFAWMGSTAAIVAFATATVLEILGYYIPFV
                     DNILDTITTPTSVVAGSLLTASTILPEISPMLKWGLAIIVGGGSAGIIQAGTALTRAT
                     STVATAGVANPVVATAEHGLSIAGSILSIIMPIVVTIIVSILIVVSLFFMIRYFKKHI
                     PTAPQPTTT"
     gene            564..1082
                     /locus_tag="PLF48_02950"
     CDS             564..1082
                     /locus_tag="PLF48_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNF48312.1"
                     /translation="MKRIGILSDTHGFFDEKIVRYFDDVDEIWHAGDIGTIAVTDKLS
                     ALKPLVAVYGNIDGTDLRTSFPEYEILEREGCKILLIHIAGTTGKYTQQTRKLIQQYK
                     PDILVCGHSHIVKVVKDENFHLLYINPGAAGVHGFHKMKTILRFEIENGKPQKMQLIE
                     LGKRGEINTNTA"
     gene            complement(1551..2747)
                     /locus_tag="PLF48_02955"
     CDS             complement(1551..2747)
                     /locus_tag="PLF48_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNF48313.1"
                     /translation="MKFIYPVLLFFAEMLLPLISLVNPKIRKWYVAQKSFKSSFTSPI
                     DKTRAYIWIHCASSGEFEQALPLIHALKSRRKELRFAVSFFSVSGYDLYRNSNLADTF
                     FNFPLDTKRNIATLIHLLQPRGVIFIRSELWLNTLLILKEQNIPVFLVNARNVMSDSV
                     FRKKYDNYCKQLFTTVFYTDKYGTTKWDKALENKKKLFQSAVLEDFTNDKFCIMVGSS
                     WNTEEAYAARFLKEYPDLKYIAFVLAPHEWDEDRLQTNFDFDRVQFFSKYEDSKRRNV
                     LFIDTKGILKYAYRYADLAFIGGGFDKTLHNALEAVVYEIPVLAGPNHQKFEEVADLI
                     QQQRIVEINSYEDFKHEVIAQMEMQKSHFLNKQDSVDEMNAQKKYASIKITEAIVKQI
                     RWEFHN"
     gene            2860..4167
                     /locus_tag="PLF48_02960"
     CDS             2860..4167
                     /locus_tag="PLF48_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNF48314.1"
                     /translation="MADNQRFIDAYISGEKLIGVIGLGYVGLPIALELARKFKVVGFD
                     INAKRIEMMQNKIDPSKELSAAAFEGCDIEFTADLDVLKKATFFIVAVPTPVDEHNVP
                     DLKPILAASTTIGKVIKKGDYVVFESTVYPGCTEEDCVPVIEQLSGLKFKKDFKVGYS
                     PERINPGDSLHTLTKITKIVSGCDVESLENIAEIYNAIIEPGVYKASSIKVAEAAKVI
                     ENTQRDLNIALMNELSQIFQLMNINTYDVLEAAGTKWNFLKFQPGLVGGHCIGVDPYY
                     LTYKSNSLGFRPQIILSGRSINDGMPQYIAKKIIQDLLKSGKNPSQCRALIMGATFKE
                     NVSDIRNSKIFDLYKELKEFQLDVDMIDAHADAHEVEEEYGVKLTDAPAGKYDIIIVA
                     VPHHAYLHMDESDFAEMANNDAIVVDLKNLYKGKIKSLNYWTL"
     gene            4172..>4974
                     /gene="rfbB"
                     /locus_tag="PLF48_02965"
     CDS             4172..>4974
                     /gene="rfbB"
                     /locus_tag="PLF48_02965"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNF48315.1"
                     /translation="MKTILITGGAGFIGSHVVRLFVTKYPDYKIVNLDKLTYAGNLEN
                     LKDIEQAPNYAFEKIDITDANAVSQLFQKYSFDGVIHLAAESHVDRSIHNPLEFIMTN
                     VVGTVNLLNAAKEAWKQDYSNKLFYHVSTDEVYGSLGEEGLFTETTPYDPRSPYSASK
                     ASSDHFVRAYQNTYDLPVVISNCSNNYGPFQFPEKLIPLFIHNIRNNKPLPVYGDGKY
                     TRDWLYVEDHAKAIDLIFHQGKNGETYNIGGFNEWQNIELIKLLCKIMD"
     assembly_gap    4975..5074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5075..7669
                     /locus_tag="PLF48_02970"
     CDS             <5075..7669
                     /locus_tag="PLF48_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNF48316.1"
                     /translation="IEDTLQIKQGEIKVEEVNKSNKKAETFAPRTKDADEQQGAIGNA
                     EKAGEVKSGQNAPDKLLKEAQKASETVEKLTATATKELRDKLDSVDIYGFNYFRQKDV
                     KIFNNAVDIKPPSNYILGVGDQIVVSIWGYADYNRLFYIDKDGYIQQENIGRVYLKGL
                     TLEQAKELLRTRFASAYMIDKSQFDVSLNYSRVITINVVGDVENPGSYTFPAINTAYN
                     ILAYVGGPSKSGTIRDIQIKRDGKIVRRFDLYKFLFYPEKQDDIFLDNNDYIYVPAQK
                     NIVRVEGAVNKEGKYEMLPGETFDDLLKYCGNFKPDAFTKVIQIRRFENNKQILLDID
                     LDSMKRIGKRVELFNGDVVGVRSIPEKVKNYVDVFGSVLLPGKYQWKEGDRVWDLIKK
                     AQGLKEDAYLEEAYVVRTNYDDFRKHTFKINLNNIISDINSPDNIPLAFRDVLEIYSK
                     TDYFDKFDIEVKGEVRKPRHFESEKGMSLKDALIMCGGLKEEALLDKIIIYRVNDDLT
                     EKLISFKIDTTNNFENMDTFLLKKRDKIYVLKDLRRIDRYYIEVNGMTRINDTFPYME
                     GMTLADAIILAKGFKMEAAANRIEIARIANYDKAISQSVPTQVTILSYSISKNVGKDP
                     VANSIKLQPFDQIFVRPTPEFEYQMKVSVKGEVLYPGEYVLTTKDEKISSLIERAGGL
                     TRFAYPDGATLNRSENGIGFVLLDLNKALANKNSKYNYVLKKGDSITVPAIKDLVTLK
                     GQFNYPSEKRPENLYVPYTDGKDAKFYITKYGAGFSDSAFARKTYVIRPNGQILRTHR
                     SMFGKVFPNVEKGSVIVVPEKEKKEKKKKEKNSDPQKFNRILNASIGAATSLLTLYLL
                     FLTVRNAK"
     gene            7659..8771
                     /locus_tag="PLF48_02975"
     CDS             7659..8771
                     /locus_tag="PLF48_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48317.1"
                     /translation="MPNNADENITEEKDTLLSNVQNNNSDIGSLEQDQSLKNIIRILK
                     ELTDIAKTYSFFLAIPVVLMALYGFYKSKQVKPTYTAKITFFLSEDRPQIPGFGAGNF
                     TNILQTTNNNFNNPKKLKEYAFTEKVGSKLLFKKYYFRGKEDYLANHYLNLFNNLQGS
                     FFKDFTNVKDLNTQDYLIFKRILGGIRQMVTVDYNEAEIYSITVTTGDEEFSKLLCEA
                     FYENVIAYYIERATGKAKITVDFLEEKLALVKAQLENSEYNLADYKDKANNLITFKAD
                     LIETRYIRNKSLLEGLYAESARGLEAAKTNYQSILPLFSTIDEPHLPLQTNVTSTLNQ
                     TLMFIGIGFFLDLLIIGFIYFRTNFWVQLKAMFKSA"
     gene            8780..9328
                     /gene="rfbC"
                     /locus_tag="PLF48_02980"
     CDS             8780..9328
                     /gene="rfbC"
                     /locus_tag="PLF48_02980"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNF48318.1"
                     /translation="MNITPTPLQDLFIIEPRVFEDERGFFFESYSEQQLTKNGLPYNF
                     IQDNHSKSAYGVLRGLHFQNAPYAQAKLVRVVEGSVLDVAVDLRMDSPTYLQHFSLEL
                     SAENKKQLLIPRGFAHGFVVLTPTCEFLYKCDNVYDKASEGGIAYNDPQLNIDWKIPA
                     SAIILSDKDKHNPTLKDAYYSF"
     gene            9335..10105
                     /gene="cysQ"
                     /locus_tag="PLF48_02985"
     CDS             9335..10105
                     /gene="cysQ"
                     /locus_tag="PLF48_02985"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNF48319.1"
                     /translation="MLTEIIKIAQDAGDAILQVYNQPVEVEIKSDNSPLTLADKKSNE
                     IIIAGLNKFFPTIPVISEETKLTEYAVRKEWTSCFIVDPLDGTKEFIKRNGEFTVNIA
                     LTENGVPVLGVIYLPAKEITYFAEKGKRCFKLEQNTKTQLHVREPAEDGLLKIVGSRS
                     HQTPELLAYVDEKKQQFKEVEFVAAGSSLKFCLIAEGKADIYPRLGPTMEWDTCAGQI
                     IAAEAGAITTVFNTDETMVYNRENLLNPYFIVKSKDCP"
     gene            10141..10653
                     /locus_tag="PLF48_02990"
     CDS             10141..10653
                     /locus_tag="PLF48_02990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF48320.1"
                     /translation="MKIRIAFFTDIPKIIEVAALVWKQTYRAFLSDEQVEYMYARMYN
                     ADAIKEQMQQGCTYLLYLDEQQIQGFVAYEIKGDVYGILKEDIIYLHRLYVRQDIHKK
                     GVGSALLQAVENVAIKSHCPYIQLNVHRKNPAMYFYKKMGFELYEKTDIAYGPFWLND
                     YILRKKITKV"
     gene            complement(10643..11323)
                     /gene="lepB"
                     /locus_tag="PLF48_02995"
     CDS             complement(10643..11323)
                     /gene="lepB"
                     /locus_tag="PLF48_02995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNF48321.1"
                     /translation="MLNKITRIVGITIGVFGILYILLRAIGYIGFYKVPTTGMYPSLK
                     PGDYFFVTKFIKPKRGDIYVYNTTFENRKNEKIVRRIAAVAGDTLALIDGYLFINHQL
                     CDINYSIALPYLVPIEKQENRPEEFFNMNTIPFNAEYSIQFLTKQEAEKNNYTIAVRE
                     IESNDYNDSLLFMNKQLEWKIGNYGPIVIPQNKFFFLGDNRYNSYDSRYTGYVDKADL
                     IGGVLIIL"
     gene            complement(11418..13229)
                     /locus_tag="PLF48_03000"
     CDS             complement(11418..13229)
                     /locus_tag="PLF48_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNF48322.1"
                     /translation="MQTNSSVFLGDQFYNRQHLNFILSDVIHAEKLSSFEYFADHDKD
                     SMQLYLDAVDQFAKEYLFPYLVEMDRKQPELENGRIKVHPIHRKIMRLSGENGWIAAL
                     AAKDAGGLQMPSVYFTSSSFILGAANYSGCVFAGLTMGAAHLIEAFADNKYHDVYLPK
                     MFAGEWQGTMALTEPGAGSSLSDVVTTAEKQADGTYKISGQKIFISCGDSDAVDNVVH
                     LLLARIKGAFAGTKGISLFIVPRERINAEGNLEWNDVTSIGVYHKMGYKGAPIAHLSF
                     GENNDCTGWLVGEENKGLSYMFQMMNEARISVGLHATAISTAAYYASLKYAKERLQGR
                     NIAEKNPELPQIPIIQHADVKRMLLLQKTFTEGALCLELLCSYYADMEKVTQGEEKEK
                     YHLLLEILTPVAKSYPAEMSNISTGNAIQIFGGYGFTKDFIAEQYYRETRIHTIHEGT
                     TAIHGLDLLGRKMMLKGGKAVMLLMQEVMADVKKAKQHENTKAYAEKLEQKLGSLQEL
                     SMHLMGVAQQEGAEAYLSDATLYLELFGILTMGWIWLKMMNACAEIEHTDEQFKQSKQ
                     LSCDYFYEYEFPKAEGLFIRLKSKKRVTTGRAQDFID"
     gene            complement(13358..>14139)
                     /locus_tag="PLF48_03005"
     CDS             complement(13358..>14139)
                     /locus_tag="PLF48_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNF48323.1"
                     /translation="KLDEPTGFELPPNGFAVDDPGYQAPAVDGSNVEVIINPDSDRLQ
                     VLNGFNPWEGDDLKGLKLLIKAKGKCTTDHISMAGPWLRYRGHLDNISNNMLIGAVNA
                     FNDKTDNVLNEISGEYGAVPATARAYKAAGFGSVVFGDENYGEGSSREHAAMEPRHLG
                     VRAVVVKSFARIHETNLKKQGMLALTFINKDDYNKIQETDTVDIIGLTEFAPGKNLTI
                     VLHHKDGSTESFDVAHSYNEQQIGWFKAGSALNLIKQQVAG"
BASE COUNT         4606 a         2528 c         2559 g         4346 t
ORIGIN      
        1 acacccgcca tggagcgccc tggggcgccc cattttgggt gcaggcttcg cctggatggg
       61 cagtactgct gcaattgtgg cattcgcaac ggctaccgtt ttagaaatcc tgggttatta
      121 tattccattt gttgacaata ttctggatac catcactacg cctacatctg ttgttgccgg
      181 ttccttgtta acggcgtcta ctattttgcc cgaaatcagc cctatgctga aatggggctt
      241 agccattatt gttggtggag gaagtgcagg tatcatccaa gctggaacgg ctcttacacg
      301 agctacatct actgtggcta cagcaggcgt agctaatccg gtggtggcta cagctgagca
      361 tggcttgtct atcgctggaa gtatcttgag tattatcatg ccaatagtgg taaccataat
      421 agtaagcatc ttaatagtag tttctttatt ttttatgata cgttatttca aaaaacatat
      481 tccaacagca ccccaaccaa ccactacata aacaacaaaa ccaacttcaa gggaggctta
      541 ttcataagcc tctttttctt tttatgaaac gcattggcat attatccgac actcatggtt
      601 tttttgacga gaaaatagtt cgttatttcg atgatgtgga tgaaatctgg catgcaggag
      661 atattggtac aatagctgtt acggataaat tatcagcact aaaacccttg gttgcagtat
      721 atggcaatat tgacggtact gacttgcgca cttcatttcc ggagtatgaa attttagagc
      781 gtgagggttg taaaatcctc ctgatacaca ttgccggaac tacaggaaaa tatacacaac
      841 aaacaagaaa attaatacaa caatataagc ctgatatctt ggtttgcgga cactcccaca
      901 ttgtgaaagt agtgaaagat gaaaattttc atttacttta tatcaatccg ggagcggcag
      961 gagtgcatgg ctttcataag atgaaaacga tattgcgttt tgaaatagaa aacggaaaac
     1021 cgcaaaagat gcaattaata gaattgggta aacgaggaga aattaataca aatacagcat
     1081 aataattttt atataataat gatttgctat tttttaaaat gaccaagttt tagtgtccta
     1141 tttataacct gagttcgggt taagaaaaaa aagcttataa aatagttgat ttctcatttt
     1201 ttcctccaac ttaacaagga ggaggaatta taggaggtgg ttcttccgac gttgttagtg
     1261 tttagtggct atgcattagc actgcaagta gtacaagatt tgaaatcaaa accataaaaa
     1321 aataatatca ttaatgaata gaaaaaaata attcaaacgg aagaggaaaa gcatttaaat
     1381 gatccttcta cgtcagagga ctttaggaaa atatactctc taaatcaagt gttgaaacaa
     1441 taaactgctt aaataaatta gatgaaaatt ctataagttg ggtttcttat agttttgaag
     1501 atctggccta taaatttcaa agtaaagaat ttatagactg tataaagaat ttagttgtga
     1561 aattcccatc gaatttgttt tactattgcc tctgtaattt tgattgaagc atattttttt
     1621 tgtgcattca tttcatccac tgaatcttgt ttgtttaaga agtggctttt ttgcatttcc
     1681 atttgagcaa ttacttcatg cttaaaatcc tcatacgaat tgatttcaac tatcctttgt
     1741 tgttgaatta aatctgcaac ttcttcaaat ttttgatgat taggcccagc cagtaccgga
     1801 atttcatata caacggcttc aagcgcatta tgcaacgttt tatcaaaacc tccgcctatg
     1861 aatgccaaat ctgcatagcg atacgcgtat tttaagattc cttttgtatc tataaataag
     1921 acattccttc gcttagaatc ttcatatttt gaaaaaaact gcaccctgtc aaaatcaaaa
     1981 tttgtttgca atctatcctc atcccattca tggggagcta aaacaaacgc gatatatttt
     2041 aagtcagggt attctttcaa aaaacgcgca gcataagctt cttctgtatt ccaactgctt
     2101 ccgaccataa tacagaattt gtcatttgta aaatcttcga gtacagcaga ttgaaatagt
     2161 ttttttttat tttcgagtgc tttatcccat ttcgtcgtgc catatttgtc ggtgtaaaaa
     2221 acagtagtaa aaagctgttt acaataatta tcatattttt tacgaaaaac agaatccgac
     2281 atgacatttc tcgcattcac taaaaaaacc gggatatttt gttcctttaa aataagaagc
     2341 gtattaagcc agagttcact gcgaataaaa attacacctc tcggttgtag caaatgaatc
     2401 aatgtcgcaa tgtttctttt tgtatctaac gggaaattaa aaaaagtgtc tgccaagttc
     2461 gagtttctat acaaatcata tcctgaaaca gaaaagaaac ttacagcaaa tctcaattcc
     2521 tttcttcttg attttaaagc gtgtataagt ggtaatgcct gctcaaattc accggatgaa
     2581 gcacaatgaa tccaaatata tgcacgggtt ttgtcaatcg gggatgtgaa actggattta
     2641 aatgattttt gagccacgta ccatttcctg atttttggat ttaccaggga aatcaaaggc
     2701 agcagcatct ctgcaaaaaa caataaaacc ggataaatga acttcatgtc tggaaaaaat
     2761 gctatatgat gcaacgaaaa ggcaatttaa gcaaacttat tgtttattaa cggttaattc
     2821 cgtaaaaatt tcgtattttc gcacttccac tatactaaaa tggcagataa tcagcgtttt
     2881 atagatgcat acatttccgg agagaaactg ataggcgtaa taggtttggg gtatgtcggt
     2941 ttacctattg cattagaatt agctcgcaaa tttaaagttg tcggttttga catcaatgcc
     3001 aagcgcattg agatgatgca aaacaaaata gaccccagca aggagttgtc tgctgcagct
     3061 tttgaaggat gtgatataga atttacagca gacttagacg tactaaagaa agccaccttt
     3121 ttcatagttg ctgttcctac cccggttgac gagcataatg taccggattt aaaaccgatt
     3181 cttgccgcat ctacaacgat aggcaaagtg attaagaaag gggattatgt cgttttcgaa
     3241 tctactgttt atccgggttg cacagaagaa gattgtgtac ccgtaatcga acagctttca
     3301 ggtttaaaat tcaagaaaga ttttaaagta ggttattccc ccgagcggat aaatccggga
     3361 gactctttac atacactgac aaaaatcact aagattgttt ctggatgtga tgttgaatct
     3421 ttagaaaaca tcgcagaaat ttataatgcc attatagaac cgggtgttta taaagcctct
     3481 tcaatcaaag tagcggaagc tgccaaagta attgaaaaca cacagcgcga tttgaatatc
     3541 gcactgatga atgaattatc tcagattttc cagctgatga atataaacac gtatgatgta
     3601 ttggaagcag caggcactaa atggaatttc ttaaaatttc agccgggttt ggtaggaggg
     3661 cattgcatcg gggtagatcc ttattacctc acatataaat ctaattcctt aggctttcgc
     3721 cctcagatta ttttgagtgg aagaagtatt aatgacggga tgcctcaata tatcgccaaa
     3781 aagattattc aggatttatt gaaaagtggt aaaaaccctt ctcaatgcag agcattaatt
     3841 atgggtgcta cctttaaaga gaatgtaagc gatatcagaa actcgaagat ttttgattta
     3901 tacaaagaac tgaaagaatt ccaactggac gtagatatga tagatgcgca tgcagatgcg
     3961 catgaagtag aagaagaata cggggtaaaa ttgaccgatg ctcctgccgg caaatatgat
     4021 atcatcatag tcgctgttcc tcatcatgcc tatttgcata tggatgaatc agattttgct
     4081 gaaatggcaa ataatgatgc aatagttgtt gatttaaaaa atctgtataa aggcaaaatc
     4141 aaatcactta actactggac actttaaaca tatgaaaacg attctaatta ccggaggtgc
     4201 aggttttata ggttcacatg tagtgaggtt attcgttacc aaataccctg attacaagat
     4261 cgtgaatctg gacaaactca catatgccgg aaacttggaa aatctgaaag atattgagca
     4321 agctcctaat tatgcttttg agaagataga tataactgat gctaatgctg ttagtcaact
     4381 atttcagaag tattcctttg atggcgtaat tcatctggca gcagaatcgc atgttgaccg
     4441 ctccatacac aatcctttag aatttatcat gaccaacgta gtgggcacgg taaatctgct
     4501 aaatgcagct aaagaagcct ggaaacaaga ttatagcaac aaattatttt atcacgtatc
     4561 gacagatgaa gtttatggta gccttggtga agaaggatta tttacagaaa ccacacctta
     4621 cgatcctcgt tctccatatt ccgcctctaa agccagttcc gatcattttg ttcgggcata
     4681 tcaaaataca tacgatttgc cagtcgtgat ttcaaattgc tccaataatt atggaccttt
     4741 ccagtttccg gaaaaattaa tacctttatt tatacataat atcagaaaca acaaaccact
     4801 tcctgtttat ggtgacggaa agtatacaag agactggctg tatgtagaag atcatgcaaa
     4861 agccattgac cttatctttc atcagggcaa aaatggagaa acttataata tcggagggtt
     4921 taacgaatgg cagaatattg aattgataaa attattgtgt aagataatgg atgannnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattgag gataccttac agataaagca
     5101 aggtgaaata aaagtagaag aagttaataa atcaaacaaa aaggctgaaa catttgctcc
     5161 acgtaccaaa gatgcagatg aacagcaagg tgctattggt aatgcagaaa aagcaggaga
     5221 ggtaaaatcc gggcaaaatg ctccggacaa attattaaaa gaagcacaaa aagcctctga
     5281 aaccgtggaa aaattgacag caacggcaac aaaagaactt cgagacaagc tggattccgt
     5341 ggatatctat ggatttaatt atttccgtca aaaagatgta aagattttta ataatgccgt
     5401 agacatcaaa cctccttcaa attatattct tggtgttgga gaccaaatcg tagtttccat
     5461 ttggggatat gctgattata acagattgtt ttatattgat aaggacggtt atatccaaca
     5521 ggagaatata gggagagtct atttaaaagg acttacgtta gaacaagcaa aagaattatt
     5581 gcgcactcgg tttgcaagtg catatatgat agataaatca caatttgatg tatcactaaa
     5641 ctattcgaga gtaatcacta taaacgtggt gggtgatgta gaaaatccgg gatcttatac
     5701 ctttccggct attaatacgg catataacat tttagcttat gttggagggc caagtaaatc
     5761 aggtactatc cgtgatattc aaataaaaag agacgggaaa attgtacgtc gattcgattt
     5821 atataaattc ttattttatc ctgaaaaaca agatgatatt ttcttggaca ataacgatta
     5881 tatctatgta ccggcacaaa aaaatattgt tcgtgtggaa ggtgctgtaa acaaagaagg
     5941 aaaatacgag atgttgcccg gtgaaacttt tgatgattta ttgaaatact gtggcaactt
     6001 caaaccggat gcctttacaa aagtgataca aatacgaaga tttgaaaaca acaaacagat
     6061 actcttagat attgatctgg atagcatgaa gagaattgga aaaagagttg aattatttaa
     6121 tggagatgtt gttggagtgc gttctattcc tgaaaaggta aaaaattatg tagatgtttt
     6181 tggttccgtt ttattaccgg ggaaatatca atggaaagaa ggagacagag tttgggactt
     6241 aattaaaaaa gcacaaggat taaaagaaga tgcctattta gaagaagcat atgtagtaag
     6301 aactaattat gacgacttta gaaaacacac attcaaaata aatctaaaca acattataag
     6361 cgatataaac tctcctgata atattccatt agcattccgg gatgtgttag aaatttattc
     6421 aaaaactgac tattttgata agtttgacat tgaagttaaa ggtgaagtga gaaaaccgcg
     6481 ccactttgag tctgaaaagg gaatgtcact gaaggatgcc ttgattatgt gtggaggatt
     6541 aaaagaagaa gcactattgg ataaaattat tatctatcgt gtcaatgatg atttaactga
     6601 aaaacttatt tcattcaaga tagataccac taataatttt gagaacatgg acacatttct
     6661 tcttaaaaaa agagataaga tttatgtctt gaaggattta agacggatag acagatacta
     6721 tatcgaagtc aatggtatga cacggataaa tgacactttt ccatatatgg aagggatgac
     6781 actggcggat gcaatcattt tagcaaaagg gtttaaaatg gaagcagcag caaacagaat
     6841 agaaattgcc cgtattgcca actatgataa ggccatctct cagtctgtcc caactcaggt
     6901 tactattttg agctatagta taagtaaaaa tgtgggcaaa gaccctgttg ctaattctat
     6961 aaaattacag ccgtttgatc aaatctttgt tcgaccaacg ccagagtttg aatatcaaat
     7021 gaaagtaagt gtaaaaggtg aagtgttata tccgggcgag tatgttttaa caactaaaga
     7081 tgaaaaaata tcctctttaa tagaaagagc cggtggactt acccgttttg cctatcctga
     7141 tggagcaact ttaaacagat cagaaaatgg gattggattc gtattattag acctaaataa
     7201 agcattggcc aataagaatt ctaaatataa ttatgttctt aaaaaaggag acagtattac
     7261 tgtgcctgct ataaaagatt tagtaacact taagggacag tttaattatc catcggaaaa
     7321 gcgacctgaa aatttatatg taccttatac agatggaaaa gatgcaaaat tttatatcac
     7381 aaaatatggt gccggtttta gtgatagtgc tttcgctcgt aaaacatatg tcatccgccc
     7441 taatggacaa attcttagaa cgcaccgctc tatgtttgga aaagtatttc caaatgttga
     7501 aaaagggtct gttatagtcg tacctgaaaa agaaaagaaa gaaaaaaaga aaaaggaaaa
     7561 gaattctgat ccccaaaagt ttaataggat attaaatgct tcaattggtg cagcaacttc
     7621 cttactaact ctttatttat tatttttaac tgtcaggaat gccaaataat gcagatgaaa
     7681 atataactga agagaaagat acacttcttt caaatgtgca gaataacaat tctgatattg
     7741 gctcgttaga acaagatcag agtttaaaaa acataatcag aatactcaaa gagctaactg
     7801 atatcgcaaa aacttattcc ttttttttgg caattcctgt tgttttgatg gctctgtatg
     7861 gtttttataa atctaagcaa gtaaaaccaa cttatacggc aaagattact ttctttttat
     7921 ccgaagacag acctcagatt cctggatttg gtgctggtaa ttttactaat atcctgcaaa
     7981 caactaataa caactttaac aaccctaaaa aattaaaaga atatgccttt acagaaaagg
     8041 taggttctaa actgttgttc aaaaaatact atttcagagg taaggaagac tatctggcta
     8101 atcattatct gaatttgttt aacaacctgc aaggcagttt ctttaaagat tttacaaacg
     8161 taaaagattt aaatacacaa gactatctta tcttcaaacg aattttaggc ggaattcgac
     8221 agatggtaac tgtagattat aatgaggcag aaatttattc cataacagtt actacaggag
     8281 acgaagaatt ttctaaatta ttatgtgaag ctttctatga aaatgttatt gcctactata
     8341 ttgaacgggc aacaggcaag gctaaaatca ccgttgactt tttggaagaa aaactagctc
     8401 ttgtaaaagc tcaattggaa aattctgagt ataatcttgc cgattataaa gataaagcta
     8461 ataacctaat cacatttaaa gcagacttga tagaaaccag atatataaga aacaaatcat
     8521 tgctggaagg tctctatgct gaatctgcca gaggacttga agcggcaaaa accaattacc
     8581 aatccatttt acctttattt tcaacaatag acgaaccaca tcttcctcta caaactaatg
     8641 ttacttccac acttaatcaa acgctgatgt ttattggtat tggcttcttc ttagatctgt
     8701 taattatagg atttatttat ttcagaacaa acttctgggt acagctcaaa gccatgttta
     8761 aatctgctta atcatttgca tgaatatcac tcccactcca cttcaggatt tatttataat
     8821 tgagccccgt gtatttgaag acgaaagagg attcttcttt gaaagttaca gcgagcaaca
     8881 acttactaaa aacggactgc cgtataattt cattcaagat aatcattcta agtctgctta
     8941 tggtgtttta agaggattgc attttcaaaa tgccccttat gcccaagcta aattagttcg
     9001 tgttgttgag ggaagcgtct tggatgtagc agtcgattta agaatggact cacccaccta
     9061 tttgcaacat ttctctttag aattatctgc tgaaaataaa aaacaattgc tcattcccag
     9121 aggctttgct catgggtttg tggtgttgac tcccacatgt gagtttcttt ataaatgcga
     9181 taatgtttat gacaaagcct ctgaaggagg tatcgcctat aatgaccctc aattgaatat
     9241 agactggaaa attcctgcct cagctattat tcttagcgat aaagacaagc ataaccccac
     9301 gctgaaagat gcgtactatt ctttttgata ttttatgttg acagaaataa taaaaattgc
     9361 gcaagatgcc ggagatgcta tcttgcaggt atataatcaa ccggtagaag tagaaataaa
     9421 atccgataat tctccgctta cactcgctga caaaaaatct aacgagatca ttatagccgg
     9481 attaaataaa ttctttccaa ccattcctgt gatttctgaa gaaaccaaac ttaccgaata
     9541 tgcagtaaga aaagaatgga caagctgttt tattgtggat ccgcttgatg gtactaaaga
     9601 atttattaag cgcaatggtg agttcaccgt taatattgca ctcactgaaa acggcgttcc
     9661 tgttttgggg gtgatttatt taccggcaaa agaaatcacc tatttcgcag aaaaaggaaa
     9721 gaggtgtttt aaactggaac aaaacactaa aacgcaactt catgtacgag aacccgctga
     9781 agacggattg ctcaaaattg taggcagccg ctcacaccag acaccggaac ttttagctta
     9841 tgtggatgaa aaaaaacaac aattcaaaga ggtagaattt gttgcagccg gaagctcact
     9901 taagttctgt ttaattgctg aaggcaaggc ggatatctat cccagattag gacccacaat
     9961 ggaatgggat acctgtgccg gacaaataat agctgccgaa gccggtgcta ttactactgt
    10021 attcaatact gatgaaacta tggtttacaa cagagaaaat ctgctcaatc cttattttat
    10081 tgtaaagagc aaagattgtc cataaaacaa ttgtcggtta taaaaatgac ttgctgatgt
    10141 atgaaaatta ggattgcttt ttttactgat attcccaaaa taatagaagt ggcggctttg
    10201 gtatggaaac aaacctaccg tgcatttttg tctgatgagc aagtggaata tatgtatgca
    10261 agaatgtata atgccgatgc gataaaagag caaatgcagc aaggatgcac ctatctcctc
    10321 tatctggatg agcaacaaat acagggtttt gtggcgtacg aaataaaagg ggatgtttat
    10381 ggcattttaa aagaagacat catctatctg catcgcttat atgtaagaca ggatattcat
    10441 aaaaagggtg tcggcagcgc actcttgcag gcagtagaaa atgtggctat taaaagccat
    10501 tgcccttata tacaactcaa tgtacaccgc aaaaatccgg cgatgtattt ctacaaaaaa
    10561 atgggatttg agctatatga aaaaacagac atcgcttacg gccccttctg gctgaatgat
    10621 tatattttga ggaagaagat tactaaagta taatcagtac ccctccaatt aaatcagctt
    10681 tatctacata accagtataa cgtgagtcat aagaattata tcgattgtct cctaagaaaa
    10741 aaaacttatt ttgaggaatt actattggtc cgtagttacc aattttccat tctaattgtt
    10801 tattcatgaa taataaactg tcgttgtaat catttgattc aatttctcga actgcaattg
    10861 tataattatt tttttctgct tcctgttttg ttaaaaactg aatagaatac tctgcattaa
    10921 aagggatagt attcatattg aaaaattctt ctggtctatt ttcctgtttt tctattggca
    10981 caagataggg taatgctatc gaataattaa tatcgcatag ttgatgattt ataaacagat
    11041 atccatctat taacgccaag gtatctccgg ctactgctgc aattcttcgt actatcttct
    11101 cattttttct attttcaaaa gttgtattat acacatatat atctccacgt ttaggcttaa
    11161 taaatttagt tacaaaaaag taatctcctg gctttaagct tggatacata cccgtagtag
    11221 gaactttata aaacccaata taacctattg ctctcaacag gatatataga attccaaaaa
    11281 cgcctattgt gatacctaca attctcgtta ttttatttaa catattattt taaataattt
    11341 atttattatt tacaaacagt tagatttatc ttttctttgt attctgttat atttatcgta
    11401 tttgaattat catacagtta atctataaaa tcttgtgctc tgcctgtagt tactcgtttt
    11461 ttgcttttta aacgaataaa taatccttct gcttttggaa attcatattc ataaaaataa
    11521 tcgcagctaa gttgttttga ttgtttgaac tgctcatctg tatgttcaat ctccgcacag
    11581 gcattcatca tcttcagcca tatccaaccc atcgtcaaaa tgccgaataa ttctaaatac
    11641 aaagtggcgt cactcaaata agcttccgca ccttcttgtt gtgccacacc catcaggtgc
    11701 atactcaatt cttgtaatga acctagtttt tgttctaatt tttcggcata agctttcgtg
    11761 ttttcatgtt gttttgcttt ttttacatcc gccattactt cctgcatcaa cagcataacc
    11821 gctttgccgc ctttcagcat catttttctg cccaataaat caagcccatg tatggcagta
    11881 gtgccttcat gtatcgtgtg gatacgtgtt tcgcggtaat attgctctgc aataaaatct
    11941 ttggtaaatc catatcctcc gaaaatctga atggcattgc ccgttgaaat attgctcatt
    12001 tctgccggat atgatttggc taccggtgtc agaatttcca acagcaaatg atatttttct
    12061 ttttcctcac cttgcgtaac tttttccata tcggcataat agctgcaaag caactccaga
    12121 caaagtgcac cttcggtaaa tgttttttgc aacagcaaca tgcgttttac atcagcatgt
    12181 tgtatgattg gtatctgtgg caactcagga tttttttctg caatatttct gccttgtaag
    12241 cgctctttag catatttcaa actggcatag taagccgctg tagaaatagc agtagcgtgc
    12301 aatcccacag aaatacgggc ttcattcatc atttgaaaca tgtagctcaa gcccttattt
    12361 tcttcaccaa ccagccagcc tgtacaatcg ttgttttcac caaaactaag atgtgcaatc
    12421 ggtgcaccct tatagcccat tttatgatat actccaatgg aggttacatc attccattcc
    12481 aaattcccct cggcattgat tctttctctc ggcacgataa acaaagaaat gcctttcgtt
    12541 cctgcaaatg ccccttttat acgggctaat aacaaatgca ctacattatc aacggcatcg
    12601 ctgtcgccac aagaaataaa tattttctgt cccgaaattt tgtaggttcc atctgcttgt
    12661 ttttcggctg ttgttactac atcgctcaat gaactgcctg cgccgggttc tgtcaatgcc
    12721 atagtaccct gccattcccc ggcaaacatt ttcggcaaat aaacatcgtg atatttatta
    12781 tcggcgaatg cttcaattaa atgtgccgcc cccatcgtta gtccggcaaa aacacaaccc
    12841 gaatagttgg ctgctcccaa aataaaactg gatgaagtaa aataaacact gggcatctgt
    12901 aagcctcctg catctttagc cgccaatgcc gcaatccaac cgttttcgcc gcttaggcgc
    12961 attatttttc gatgaatggg atgcaccttg attcttccat tttcaagttc aggttgcttt
    13021 ctgtccatct ctaccagata aggaaaaaga tattctttgg caaactgatc tacggcatcc
    13081 agatatagct gcatactgtc tttatcgtgg tctgcgaaat attcgaatga agaaagtttt
    13141 tcggcatgaa taacatcaga caagataaaa tttaaatgct ggcggttata aaattgatca
    13201 ccgagaaaaa cagaagagtt ggtttgcatg cgctgaaaga ttaaatgaat aattgattaa
    13261 tttcggaaaa tacgacaatg tatgcaaata aaaaagccgc caaaatttgg cagcttttat
    13321 aaataaattt tacagtttaa atgttcttat ctgttactta ccctgctact tgctgtttta
    13381 tcagatttaa ggcactgccg gctttgaacc aaccgatttg ttgttcatta taagaatggg
    13441 ctacatcaaa actttctgtg cttccgtctt tatgatgcag tactatcgtc aggtttttgc
    13501 cgggtgcaaa ttctgttaat cctattatat caacggtatc tgtttcttgt atcttgttgt
    13561 aatcgtcttt attaataaaa gtcaatgcca gcataccttg ttttttcaag ttggtttcat
    13621 gaatacgggc aaacgatttt acaactaccg cgcgcacgcc taaatgacgt ggttccatcg
    13681 ctgcatgttc tctggatgaa ccttcgccgt agttttcatc gccaaataca accgaaccaa
    13741 atcctgctgc tttatatgca cgggcagttg ccggcacagc accatattct ccgctgattt
    13801 catttaatac attatctgtt ttgtcattaa atgcatttac cgcacctatc aacatgttgt
    13861 tggagatatt atccagatgg cctctgtagc gcaaccaggg acccgccata gaaatatggt
    13921 cggtggtaca tttacctttt gcttttatta ataattttaa ccctttcaaa tcatctcctt
    13981 cccatgggtt aaaaccgtta agtacctgta agcgatctga atcggggtta ataatcacct
    14041 ccacgttgct accatctaca gcaggcgcct gatagcccgg atcatctacc gcaaatccat
    14101 ttggtggcaa ttcaaatcct gtaggttcgt ctaatttta
//
LOCUS       DAOQII010000036        14001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2109_length_14001_cov_4.724365, whole genome shotgun sequence.
ACCESSION   DAOQII010000036 DAOQII010000000
VERSION     DAOQII010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_14001_cov_4.724365"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLF48_03010"
     CDS             <1..1185
                     /locus_tag="PLF48_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF48324.1"
                     /translation="KKVVEINSKTKEQIEVLEVDKEQQEQYTLTHNFEKIETLDDFNG
                     KWRDFEDDEDLDESSEALEELNMNQMVRVDSPSHSVYHAEFFNSKGIKESKEKQDETV
                     RKKVDEWDFQRKKYKKNYCNIYESVFRETKPGYTSYTFAKNKLILKRLEKNLNQFFNA
                     YQKVNRQADGDEPDIDAVTERFTDIYSGFSPGENIFITKRKRVKDVSILILTDISLST
                     DGYVEGQRILDTEKQSILLFCEILNKYGTPFQIDVFSSKTRNHCEYATVKTFSEDWNN
                     VKHRISALSPKGYTRIGAAIRNAHEQVKKNNARRKWILLLTDGKPNDYDRYEGKYGIE
                     DIKQAVREVRQNNIQISALAIDSNAKFYLPQMLGKGAFQLVHHPNKLPEALTQFYLEL
                     MK"
     gene            1216..1908
                     /gene="ric"
                     /locus_tag="PLF48_03015"
     CDS             1216..1908
                     /gene="ric"
                     /locus_tag="PLF48_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNF48325.1"
                     /translation="MENQTIAEIVAQNYQTADVFKKYEIDFCCGGKKTLEAVCAEKNL
                     PFEKINAELNEKTSLMRDMPNYNAWKLSFLIDYILQVHHQYITESIPVIRQYADKVTT
                     VHGNRHPELAHIRNLFYEVAAELEAHLMKEERILFPYIKQAEENKGEINAPFGSIKNP
                     IAMMEHEHDKAGDLCHQIAVLTNQYSVPEDACNTYRVYFAKLKEFEDDLHKHVHLENN
                     ILFAKAIKLSEK"
     gene            complement(1911..2654)
                     /locus_tag="PLF48_03020"
     CDS             complement(1911..2654)
                     /locus_tag="PLF48_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF48326.1"
                     /translation="MLSQVVIKHINSLKIKKYRNKYSEFIAEGEKIIAELIEEGLILQ
                     QIYATTASTTVFSDKFQSLLIQDKMTVCTDAELNKITALQHHHQCLAVFSQPDSAFEP
                     DESNWILALDDIQDPGNMGTIIRTCDWFGIKHIVCSEGCVDIYNPKVVQATMASIGRV
                     NVIETDLADFFSKSNREVYGAVLDGANYHTADYSKPGVLLIGNEGKGIKPELMQWVQH
                     KLTIPKIGKAESLNAAIATALLLAKINMS"
     gene            2657..5233
                     /locus_tag="PLF48_03025"
     CDS             2657..5233
                     /locus_tag="PLF48_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNF48327.1"
                     /translation="MQVKNYFRYISVLCALILLSGCSATKRLLEKDQSLLIANTVQLK
                     NSGKLITEKNTLKSELGKQVVYSQQPNKKFLSLFRMKLGIYTFSTFKNKKRLTKKIEL
                     EKKINNGTATDDEKKELDKLLKKKKFENFLNETSGGEPPVLFDSTTMKTTVNRMKNYL
                     FYHGFFNSDVTYSFQTKNKKTKAIYTASLGNQYTLKEIKITSEDSNITPIIQKSKKEL
                     LMRKGDAFDIDIIKKDRTLLAEAVQNEGYFVFSPEYISMNVDSTIGNKEVNVELYVNL
                     DVDSSIHKKYYYDEITINVLDYEKKDKILNQTKRDYIYDTICDVAYKTDKKTVFPRAL
                     SKSIYIHKYELYSKSNLDKTKSALNNLGIFKYVNVEYEPLSFSPDEISLITRINCSPA
                     KRHSFNLDNELNTNAKSTLGFAITGGYTNKNLFKSAAKFQLNFSAGVEFQLLKENRIE
                     NSPISTININSEAKIDLARIFPTFKVRSACDKTYKYKPRTFIALNYSFQRRIGLYDIH
                     TVGLNYGYEFYNSKFRHTFSPLSFTYVNPTKITEKFDTTYLTNNARLAQSFQKQFIIG
                     QDYTFSYNNQNLNVGRYKNYFYFRGNAFFSGNIVSLFSMLIKKDEAKPRKVGGIPFSQ
                     FFRLELEPRYSFNFKRGQTVALRMFVGAGIPYGNSSYTTIETDKNDSTYTREVAVLPY
                     VRQFYSGGPNSLRGWQFRKVGPGGYNIYKTLKNDLDQTADLKFEFNAEYRFNIYKFFN
                     GALFTDIGNIWLLKEDPNKPYANFAASRFMKELAWDAGAGVRMDFNFFVLRFDVGFVL
                     YDPSFDAGDRWVFNKIDNPDYQLNTTRKFGRGRNAEKIQGFYNFKFKDFVGLNFAIGY
                     PF"
     gene            complement(5257..6327)
                     /locus_tag="PLF48_03030"
     CDS             complement(5257..6327)
                     /locus_tag="PLF48_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48328.1"
                     /translation="MKYCFRIHLLLLLALVQFTSCNREDNPNPNIIYSTSGIYLAGTE
                     VSAGRYKAVYWKNDSIVYLGDSSKNMQATGIFVTDEDVYVLGYEILGSNNRILYWKNG
                     IATYLTDGTHDAMPSSILVSNNDVFVSGTENNGSKLVAKYWKNNISVSLTNGTQNATA
                     NAMCIANGDVYIAGHESNGSHTVATYWKNGNAVRLTDGTGDAILYSISVDGTNVYTCG
                     HEWNTDWSILNPRLMARYWKNGTAFTLYDSTSAILSGMATSIFAKNNNVYTTAVQYSG
                     SILNSNTDARFWSNNSSFPLNNAISNLLTTALYNKDGDIYIIGNETVSNNVYKPVLYK
                     NGIEQSVSYTTPSALVYGLFVK"
     gene            complement(6734..8863)
                     /locus_tag="PLF48_03035"
     CDS             complement(6734..8863)
                     /locus_tag="PLF48_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNF48329.1"
                     /translation="MFHYTKDQDNIVTITMDMQGRSANVINEEFGKLFNETLAKLEAE
                     KNDIAGVILTSAKSTFFAGADIDNLYKQTDAKLIFDMCEQLKAQFRRLETFGKPVVAA
                     LNGAALGGGLELALACHYRIALNNPKAQFGLPEVGLGLLPGGGGIIRLTRMIGLQPAL
                     PLLTEGKRLKVKDAMANGLINEIADSPEDMITRAKAWIKAHPKAINPWDDKAFKFPGG
                     DARNPKVASMIPIVPAMTKKKTHGNYPAVEAIIDTAVEGSVVDFDTACRIESRYFTSL
                     AVGQVSKNMITAFWYNLNAINAGGSRPKGFEKYTTKKVGVLGAGMMGAGIAYVSALSG
                     MEVILKDISVEAAEKGKAYSEGLLKKSISKGKMTKEKAEEILNRIHATDKPDFDGCDL
                     IIEAVFEDRALKAKVTQEAEATMLAVGTFASNTSTLPITGLAQASARPKQFIGLHFFS
                     PVDKMPLVEIICGQETDDATLAKAFDYVLQIKKTPIVVNDSRGFYTSRVFSTYVMEGI
                     SMLLEGQHPRVIENAGLKAGMPVGPLALSDEVSLSLMDHIRKQTIKDLEAEGKEIPHH
                     PGYEVIEKMLSINRKGKAAGAGFYEYPQGGTKFIYPELVTIFPQAAEVPQQEMIDRML
                     MVQALDTVRCLEESVLRSVADANIGSIFGWGFAPFQGGTLQYLNGMGLNNAIEKAKKL
                     AAKYGERFSVPKLLQEKADKGELFTDN"
     gene            9779..11359
                     /locus_tag="PLF48_03040"
     CDS             9779..11359
                     /locus_tag="PLF48_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNF48330.1"
                     /translation="MTPEQSQELIEYGARRTIITITVVLCTLLELIDTTVVNVATTTL
                     MGNLGATVSEVSWVIASYAIANVIVVPMSGWLSSFFGRKYYFAGSVALFTFSSLMCGL
                     SGSIWTLVFWRFIQGIGGGALFSTSQTILKEIYPPEKIGMAMAMFGMGVIVGPTIGPY
                     LGGYLVYNYSWPVIFYVNIPIGIVATFLTLTYIRDNPYDTKPEGSIDWLGIFLLILGI
                     GSLQLILEQGERNDWFESKFILTFTAFTIIGLFGFILRELTAKNPIVDLRVLTKGNVA
                     IGTFMNFILGFILFGSVFIYPIYMQRFLGFTAQMSGAMFIPGALLSGMLMPVIGTLLS
                     KGMPPKLMIMFGFMTTAVFVFWSSFIFTTNTGADAMFWPLIVRGFGMAFLFVPLSGLT
                     LGGLMGKDVGQASGISNMIRQIGGSISVAVIGVLNETLSAKHRADLLTHITPENVTVM
                     ERINMMKAGMLSATADADKALQQAYAYLEFTIDKQAAILSYIDIFRWMALVIVLSVPV
                     VLLARTYKFDKDQMNEGH"
     gene            11630..12907
                     /locus_tag="PLF48_03045"
     CDS             11630..12907
                     /locus_tag="PLF48_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF48331.1"
                     /translation="MKLKQLLVLVLFVPSFCLAQEQSQLYQILFKKNEFKLSEINKQF
                     LSAVSDSLLGKSNFIIYINGHADSDADSSYNYTLSLNRSIAVKNYLTEKGVNDSLFII
                     QALGEENPLVANTTPIEKAKNRRVELYVLYRNYEKKEIIYPKYYDTVTDCNRDTLVEL
                     ESGYKVLLSVCDWKKNKKCLTIKKSLNYKFISKENWLKKHLGFKNYEKIIRCEPHYNF
                     HILSCQDSCFSRPAKLYIPQFQAPGLTTSAGASQKKNNKNAKIKLVFKQTKIGSYVYY
                     VADLYCPGMINCYLDKRCTHPVSLYFKRNTRVLAYSYTENNLEYNKDTVIQINPENQK
                     KITTNYRNAFFNTILIKHHDDTIQLKDIPIELFAHIRKKINSNLGTESKSYFFFIPYR
                     KKYKCMHYTKYKLRVRDINKLQHFNLQELSGGK"
     gene            13230..13607
                     /locus_tag="PLF48_03050"
     CDS             13230..13607
                     /locus_tag="PLF48_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48332.1"
                     /translation="MKNKLINFNHEDFEIVRNIALSFPYTEESISHEGTPSVKVCGKL
                     MCRLHECGEYIPIRLDFEIREKYLENYPEIFHVPEHFKAYPYICMWKHNYDINLLMEI
                     LEQSWKGLATQKQVKEYYERKKE"
     gene            complement(13658..>14001)
                     /locus_tag="PLF48_03055"
     CDS             complement(13658..>14001)
                     /locus_tag="PLF48_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNF48333.1"
                     /translation="FKLIKAFGIDEYTDWSAMEAYDEYCDYFLFDTKTALHGGSGLAF
                     NWDLLSHYSLIKPLFLSGGIGVDDIPRIKQLAQKTSVEIVDLNSKLEISRGIKDIGLC
                     RQAIEFCKKQE"
BASE COUNT         4653 a         2521 c         2451 g         4376 t
ORIGIN      
        1 aaaaaagtgg tagagataaa ctcgaaaaca aaagaacaaa tcgaagtatt ggaagtagat
       61 aaagaacaac aagaacaata caccctgaca cacaatttcg agaaaattga aaccttggat
      121 gattttaacg gcaagtggcg ggattttgaa gatgatgagg atttggatga aagcagcgag
      181 gcacttgaag agctaaatat gaaccaaatg gtgcgggtag attcgccgtc tcattcggta
      241 tatcatgctg aatttttcaa ttctaaaggc ataaaagaat caaaagaaaa acaagatgaa
      301 acagttcgta aaaaggtaga cgaatgggat ttccaaagaa aaaaatacaa gaaaaactat
      361 tgcaatattt acgagtctgt ctttagagaa acgaaaccgg gatacacgag ttacacgttt
      421 gctaaaaaca agttgatatt aaagagactc gagaaaaatt taaatcaatt ttttaatgct
      481 taccaaaaag taaatcgtca ggcagacggc gatgaacccg acattgatgc agttactgaa
      541 agatttacag atatttattc cggattttcc ccgggtgaaa atatatttat cacgaaacga
      601 aaaagagtaa aagatgtgtc tatcctaatc ttaaccgata ttagtttatc tactgatggc
      661 tacgtggaag gtcagcgcat cctcgacacc gaaaaacaat ctattctctt gttctgcgaa
      721 attttaaaca aatacggcac gccttttcaa attgacgtat tctccagcaa aacacgtaat
      781 cattgtgaat atgcaacagt caaaacattt tctgaagatt ggaacaatgt aaaacaccgc
      841 atcagtgcac tctctccaaa gggctacacg cgcataggtg ctgctattcg aaacgctcac
      901 gaacaagtga aaaaaaacaa tgcgcgcaga aaatggatct tactgctaac agatggcaaa
      961 cctaatgatt atgacagata tgaaggcaaa tatggcattg aagacataaa acaggcggtt
     1021 cgtgaagtgc gtcaaaacaa catacagatt tccgcgcttg ccattgacag taacgcgaag
     1081 ttctatctgc ctcagatgct tgggaaaggt gcattccaac tggtacatca cccgaataaa
     1141 ttgcccgaag ccttaacaca attttatctg gaactaatga aataatttta ttcataaaca
     1201 tgtaaattta atatcatgga aaatcaaact attgctgaaa tcgtggcaca aaactatcaa
     1261 accgctgatg tattcaaaaa atatgaaatc gatttttgct gtggcgggaa gaaaacactt
     1321 gaggccgtat gcgctgaaaa gaatctgcct tttgagaaaa tcaatgccga attaaatgaa
     1381 aagacttcac ttatgcgcga catgcctaat tacaatgcat ggaaactgtc atttttaata
     1441 gactatatct tacaagtgca tcatcagtat attacagagt caatacctgt gattcgtcag
     1501 tatgcagata aagtgaccac ggtacatggc aacagacatc ctgagttggc acatatcaga
     1561 aatttatttt atgaagtagc tgctgaactg gaagcacatt taatgaaaga agagcgcatt
     1621 ttatttcctt acatcaaaca agcggaagaa aataagggag agatcaatgc cccattcgga
     1681 agtataaaaa atccgattgc catgatggaa cacgagcatg acaaagcagg agatttatgc
     1741 catcaaattg cagtattaac caatcaatac agtgttcctg aagatgcctg taatacttat
     1801 cgggtttatt ttgccaagct aaaagaattt gaagacgatt tacataaaca tgtacattta
     1861 gaaaacaata tcttgtttgc taaggctatt aagctaagtg aaaaataaaa ttacgacata
     1921 ttaatcttcg ccagcaataa cgccgtagca atagctgcat tgagcgactc tgcttttcct
     1981 attttgggaa tggtcagttt atgttgtacc cactgcatca gttctggttt tatgcctttt
     2041 ccttcattgc caatcagcaa cactcccggt ttgctgtagt ctgccgtatg ataatttgca
     2101 ccatctaaaa ccgcaccata tacctcccga tttgattttg aaaaaaaatc agccaaatcg
     2161 gtttcaatta cattaactct tccaattgat gccatagttg cctgtactac tttcgggtta
     2221 taaatatcaa cacatccttc agaacatacg atatgtttta taccaaacca gtcgcaagtg
     2281 cggataatgg tacccatatt gccgggatct tgtatatcat ccaaagctaa aatccagttg
     2341 gattcatcgg gttcaaaagc tgaatctggc tgagaaaata cagccaaaca ctggtgatga
     2401 tgctgtaatg ctgttatctt attcagctcc gcatctgtgc agacagtcat tttatcctga
     2461 atcaacaggg attgaaattt atcggaaaat actgtagtgc tagccgtagt ggcgtaaatt
     2521 tgttgaagta taagcccctc ttctattaat tcagcaataa ttttttcgcc ttcggcaata
     2581 aattcggaat acttattgcg gtatttttta attttgagcg aattaatatg cttaatgaca
     2641 acctgactta acacgcatgc aagttaaaaa ctatttcaga tatatttcgg ttttgtgtgc
     2701 gctcatccta ttgagtggtt gcagtgccac taaaaggttg ctggaaaaag accagagtct
     2761 gctcattgcc aataccgtac agttgaaaaa cagcggcaaa ctgattactg aaaaaaatac
     2821 attaaaatca gaattaggaa aacaagtagt ttacagtcag cagccgaata aaaaattcct
     2881 gagtttattc agaatgaaat tgggtattta tactttttca acttttaaaa acaaaaaaag
     2941 actaaccaaa aaaatagagc tggagaaaaa gataaataac ggtacggcta cagatgacga
     3001 gaaaaaggaa ttggataaat tactcaagaa aaagaaattt gagaacttcc taaacgaaac
     3061 ctccggaggc gagccaccgg tgttgtttga ctccactacg atgaaaacga cagtaaaccg
     3121 gatgaaaaat tatttgtttt atcacggttt ttttaattca gacgttacat acagttttca
     3181 aacaaaaaac aaaaaaacaa aagccatata cactgcttct ttaggcaatc aatacacgct
     3241 taaggaaatt aaaattacgt ctgaagattc aaacatcacg cctatcatac aaaaatcaaa
     3301 aaaagaatta ctgatgcgca agggtgatgc atttgatatc gacattatta aaaaagacag
     3361 aaccttattg gcggaagcag tgcagaatga gggctatttt gtgtttagtc cggaatatat
     3421 ttctatgaat gtggatagca cgataggcaa taaagaagtt aatgttgagc tttacgtaaa
     3481 tttagatgtg gattcttcca ttcataaaaa atactattac gatgaaatca ccatcaatgt
     3541 actggactat gaaaagaaag ataaaatatt aaaccaaacc aagcgcgatt atatttacga
     3601 tactatttgc gatgtagctt ataaaacaga taaaaaaaca gtttttccac gggcattatc
     3661 aaaatctatt tacatacata aatatgaact atacagtaaa tctaacttag ataaaacaaa
     3721 gagtgcttta aataatttag gtatttttaa gtatgtaaat gtagaatatg aaccactttc
     3781 attttcacct gacgaaatat cactgattac tcgtataaat tgctcgccgg caaaaagaca
     3841 ttcattcaac ttagataatg agcttaatac caatgccaaa agtacacttg gatttgccat
     3901 taccggaggt tataccaata aaaatttatt taaatctgcc gccaagtttc agttgaattt
     3961 cagtgcgggt gtggaatttc agttattaaa agaaaatcgt attgaaaaca gtccgataag
     4021 caccatcaac ataaacagtg aagcaaaaat tgatttagcg cgtatatttc ccacttttaa
     4081 agtgcgttct gcctgcgata aaacatataa atacaaacct cgtacattta ttgcattaaa
     4141 ttacagcttt cagcgccgca tcggtttata tgacatacat accgttggtt tgaattatgg
     4201 ctatgagttt tataacagca aattcaggca taccttttca cctttgagtt ttacttatgt
     4261 caacccgact aaaatcaccg aaaagtttga caccacttac ctcacgaata atgcccgcct
     4321 ggcacaaagt tttcaaaaac aatttatcat cgggcaagat tatacgtttt cttacaacaa
     4381 tcaaaatttg aatgtaggtc ggtataaaaa ttatttttat ttcagaggca acgcattttt
     4441 ttcgggcaat atcgtttcgc tattttcaat gcttattaaa aaagacgaag ctaagccaag
     4501 aaaagtggga ggaattcctt tttctcaatt tttcaggtta gaattagaac ctcgttactc
     4561 tttcaatttc aagcgaggcc aaactgtggc tttgcgcatg tttgtaggtg ccggtattcc
     4621 gtatggcaat tcttcgtaca ccactatcga aacggataaa aatgactcta cttacactcg
     4681 agaagtagca gtattacctt atgtacgaca gttttattca ggaggaccca attcgttgcg
     4741 tggctggcaa ttccgcaagg ttggtcccgg tgggtataat atatataaaa cattaaaaaa
     4801 tgacttggac caaacagcag atttaaaatt tgaatttaat gcagaataca ggtttaatat
     4861 ctataaattt ttcaatggtg ctttgtttac agatattggg aatatctggt tgctgaaaga
     4921 agacccaaat aagccgtatg ccaattttgc tgcctcgcgg tttatgaaag aattagcctg
     4981 ggatgctggt gcgggagtgc gtatggattt taactttttt gttcttcgtt ttgatgtagg
     5041 ttttgttttg tatgatccct cttttgatgc gggagacaga tgggtcttta ataaaataga
     5101 caatcccgat taccagctca atactacgag aaaattcggc agaggcagaa atgcagagaa
     5161 aatacagggg ttttataatt ttaaattcaa agatttcgtt gggttaaact tcgctatcgg
     5221 gtatccgttt taatttgttt aatacacgac atattttcat ttaacaaata gtccatatac
     5281 caaagcagat ggagtggtgt aagatacaga ttgttctatt ccatttttat acaaaactgg
     5341 tttataaaca ttatttgaaa cggtttcatt tcctataata taaatatctc catctttatt
     5401 ataaagagca gttgtcaata aatttgaaat agcattattt aaagggaaag aagaattatt
     5461 cgaccaaaat cgtgcatctg tattagaatt caagatactt ccagaatact gcacagctgt
     5521 tgtataaaca ttgttattct ttgcaaaaat agatgttgcc atacctgaca aaattgcact
     5581 tgtactatca tataaagtga atgcagtacc atttttccag taacgagcca ttaatctagg
     5641 attgagaata ctccagtctg tattccattc atgtccacat gtatacacat ttgttccatc
     5701 aacggatatt gagtaaagta ttgcatctcc agtaccatca gttaatctta cagcattccc
     5761 attcttccaa taagtagcga ctgtatgtga tccattagat tcatgaccgg caatatatac
     5821 atcaccattt gcaatacaca ttgcattagc tgttgcattt tgcgtaccat ttgttagaga
     5881 cacagatata ttatttttcc aatattttgc tactaattta cttccattat tttctgttcc
     5941 tgaaacaaaa acatcattat ttgatacaag tattgaactt ggcattgcat cgtgtgtacc
     6001 atcagtcaaa taggttgcta ttccattttt ccaatacaat atcctattat ttgaccctaa
     6061 aatttcatat cccagtacat aaacatcttc atctgtaaca aatataccag tagcctgcat
     6121 atttttagat gaatcgccca aataaactat actatcattt ttccaataca cagccttata
     6181 cctgccggct gacacctcag taccagctag ataaatgccg gaagtgctat atataatatt
     6241 tggatttgga ttatcttctc tattgcaaga ggtaaattgc actaatgcca gcaataaaag
     6301 cagatgaatt ctaaaacaat acttcattct atataaattt agctcaaaga tatgtaaata
     6361 attctatact taattctggc aagaaaaaga ttattgatgt tgaattctgg ttgataatgt
     6421 aatatgataa gaaaaatggc tagactaaaa agcaaattct aaaccatcaa gaagttaaga
     6481 gtattaagtt tcacattttt caaagcaaat tctaaaccat taagaagtta agagtattaa
     6541 gtttcacatt tttcaaagca tattttaaac cattaagaag ttaagagaat taagacattt
     6601 ctcatcttca catcttcaca ttttcacatt ttcaagttaa attataaaca attaagaaat
     6661 taagacattt ctcatcttca catattcaca ttatctcatt ttcttatctt cacatttcac
     6721 attttcaaag tggctaatta tctgtaaaca actcgccttt gtcggctttt tcttgtaata
     6781 attttggaac ggaaaagcgt tcgccgtatt tagctgctaa tttttttgct ttttcaatcg
     6841 cattatttaa gcccataccg ttcagatatt gcaacgtgcc tccctgaaat ggtgcaaatc
     6901 cccatccaaa aatagaaccg atattcgcat cagccacact gcgcaataca ctttcctcta
     6961 agcaacgaac tgtgtctaag gcctgtacca tcaacatgcg gtctatcatt tcttgttgtg
     7021 gcacttctgc tgcttgagga aaaatagtta ctaattcagg atatatgaac tttgttccgc
     7081 cttgcgggta ttcataaaat ccggcaccgg cggctttgcc tttacgattt atcgacagca
     7141 ttttttctat cacctcatag ccgggatgat gcggaatttc tttaccctca gcttccaaat
     7201 cttttatggt ctgtttgcgg atatgatcca tcaaactcaa agatacttcg tcacttaatg
     7261 ccaatggacc aacaggcatt ccggctttta atcctgcatt ttctataaca cggggatgtt
     7321 gaccttccag caacatggaa ataccttcca ttacataagt agaaaatacg cgcgaggtat
     7381 aaaatcccct ggagtcgttt acaactatag gtgttttctt gatttgcaac acataatcaa
     7441 atgctttggc caaagtagca tcatcggttt cttgcccgca aataatttcg accaaaggca
     7501 ttttatctac cggagaaaag aaatgcagac cgataaattg ttttggtcgg gcagaagctt
     7561 gtgccaaacc agtaatgggc aaggtagaag tattggaagc aaatgttcct actgctaaca
     7621 ttgttgcctc ggcttcttgt gttacttttg ctttcaaggc tctgtcttca aaaacagctt
     7681 ctatgattaa atcgcaacca tcaaaatccg gtttgtcagt agcatgtatc ctgttcaaaa
     7741 tttcttcagc tttttctttg gtcatttttc ctttactaat actttttttc agcaagcctt
     7801 cggaataggc tttgcctttt tcagctgctt caacagaaat atctttcagt atcacttcca
     7861 taccggatag agctgaaaca taagcaattc cggcacccat cataccggca cccaatacgc
     7921 ctactttctt agtagtgtat ttttcaaatc ctttcgggcg agaaccgccg gcatttatgg
     7981 cattcagatt ataccaaaaa gctgttatca tatttttgga aacctgacct actgccaatg
     8041 atgtaaaata acgcgattcg atgcggcatg cagtatcaaa atcaacgaca gaaccttcaa
     8101 cagctgtatc aataatggct tccactgccg gatagttgcc atgtgttttc tttttagtca
     8161 tggcaggcac aatcggaatc atgcttgcca cttttggatt tcgggcatct cctcccggaa
     8221 atttaaatgc tttatcatcc catggattaa tggctttagg atgggcttta atccaggctt
     8281 ttgcccgagt tatcatgtct tccggtgaat cagcaatttc gttaattaat ccatttgcca
     8341 ttgcatcttt tactttcagt cgcttgcctt ccgtcaataa tggtaaagct ggctgcaagc
     8401 ctatcattcg ggtaaggcga ataatgccac cgccacccgg caatagtccg agaccaactt
     8461 caggcaaacc gaattgtgct tttggattgt tcaaagctat tctataatga caagcaagag
     8521 ccagttccaa gcctccacct aaggcggcac catttagtgc agccaccaca ggtttgccaa
     8581 atgtttccaa acggcgaaac tgagctttaa gttgttcgca catgtcaaat ataagtttgg
     8641 catcggtttg tttataaaga ttatcaatat cggcacctgc aaaaaaagtg gacttggcgg
     8701 acgtgagtat gacaccggca atgtcatttt tttcagcttc taatttcgcg agtgtttcgt
     8761 taaagagttt gccaaattct tcattgataa cattggcact tctgccttgc atatccatgg
     8821 tgatggttac gatattgtct tggtctttag tgtagtggaa catgaaatgg attttgtaat
     8881 gagataattt agtaatctga acacgtgaag ataagaaaat aagaaattat ttttaagaat
     8941 taagataaaa acatagacag aatttaccaa aattacttta agaaaatgtg tcaaaacaca
     9001 ctagaaaatt tcaaaatcct gataggttga atcaaagtga taaatgcagt tttgttgaga
     9061 cttggtttat cctggtaaat tataacatta actatttccc tgggtattat gatgtagtat
     9121 ttatcaaatg gctcaataat atacctgctt cctgaacaca taagtatact taggcgaaat
     9181 tccaagttgg gcattttcaa ttcaattcgc gatcaattct tgtgcataaa tttcaaatga
     9241 tttttttagc tacaatgttc aatttggtct ctattactaa catgaaacaa acacatgcaa
     9301 ttttaattcg ttattaacta aagcatggat gttactacaa atacaaaatg ttcttttact
     9361 attttttatt ccggtttttt ttgtgtcgta ttgtcatgat tagctttata taaacactaa
     9421 atcataactg tcttatgtct gtattatttc aactttggta aagaaaacaa attcacttta
     9481 cctgtctcaa tcaaatgcga taaattagaa ttgcaatgtc atcctgcata atattcataa
     9541 ttattttacg atttaattta tagtttatag tttacttaac aattataagt taatataaat
     9601 gcgactccac aagcaaagaa atgcacaatc tgtactaaac aaagtaaata taaacttaaa
     9661 gagttgcttt ttcattccag ctgaaatcac ttatctttgt taactaagac gcagaatccc
     9721 gcaagtgcat atttgaaaaa acttttccta tttaaacgca taccaaaaaa tataaccatt
     9781 gacacccgaa caatcacaag aactaattga atacggcgca aggcggacaa ttataaccat
     9841 tacggtagtg ctttgtacct tgcttgaact gatagatacc actgtggtaa atgttgccac
     9901 tacaacctta atgggcaact tgggtgctac cgtatctgaa gtgagctggg taattgcctc
     9961 atatgctatt gccaatgtaa tcgtagtgcc aatgagtgga tggctttctt ccttctttgg
    10021 tcgaaaatat tatttcgcag gttctgttgc cttatttact ttctcttccc ttatgtgcgg
    10081 tttgtcgggt tcgatatgga ctttagtttt ctggagattt atacaaggaa ttggcggtgg
    10141 tgcattattc tctacttcac aaactatttt gaaagaaatt tatccgccag aaaaaatcgg
    10201 tatggccatg gctatgttcg gtatgggggt aattgtcggg ccgactattg gtccttattt
    10261 gggcggctat ttagtatata attattcttg gcccgtaatt ttttatgtca atattcccat
    10321 tggtattgtt gccacatttt taacgcttac ttatatccgc gataatcctt atgataccaa
    10381 gccggaaggc agcattgact ggctgggtat atttttgctt atattaggta ttggttcact
    10441 gcaattaata ttagaacaag gagagcgcaa cgactggttt gaatctaagt tcatcctgac
    10501 gtttacggca ttcaccataa tcggactgtt tggttttatt ttgcgggagt taacggctaa
    10561 aaaccccata gtggatttgc gggtattgac caaagggaat gtggctattg gtactttcat
    10621 gaattttata ctgggtttta tcttgttcgg aagtgtattt atatatccca tttatatgca
    10681 acggttttta ggttttacgg cgcaaatgtc aggtgctatg tttatacccg gtgccttatt
    10741 aagcggtatg ctgatgcctg tcatcggtac tttactttct aaaggcatgc ctccaaaact
    10801 tatgattatg tttggtttca tgaccacagc cgtttttgtg ttttggtcat cttttatttt
    10861 tacgaccaac accggggcag atgccatgtt ctggccgttg attgtacgcg gttttggtat
    10921 ggcgtttttg ttcgtgccac tgtcgggctt aacacttggc ggattaatgg gaaaagatgt
    10981 aggacaagct tcgggcattt ccaatatgat cagacagata ggtggttcta taagtgttgc
    11041 ggtaattgga gttttaaatg aaacactaag tgctaaacat cgtgctgatt tgctgacgca
    11101 tattacaccc gaaaatgtaa ctgtaatgga gcgcatcaat atgatgaagg caggcatgtt
    11161 atccgcaacg gctgatgccg ataaggcttt gcagcaagca tatgcatatc tggaatttac
    11221 gatagacaag caagctgcta ttttgagtta tattgatatt ttcagatgga tggcattggt
    11281 gatagtatta tctgtaccag ttgtgctgct ggcgcgcaca tataaatttg ataaggatca
    11341 gatgaatgaa gggcattaag caggattagt gcttattaac ctaaattcga gataagcgtt
    11401 tctatttaaa aatagttgat tttttatata gaaccacctc ctttttattc ctcctccttg
    11461 ttaagttgga ggaaaaaata agaaatcaac tattttttta accctaactc tggttgttat
    11521 ttctatgtta gtggaaatta tcttttcatc ttactgagac ttcaggataa catagccaat
    11581 ataaaatata ttatcttgcg gaaacaacaa gattttaatt caacactcaa tgaaattaaa
    11641 gcagctactc gttttagtct tatttgtccc cagtttttgc cttgctcaag aacaaagtca
    11701 gctatatcag attttattta aaaagaatga atttaagctg tctgaaataa ataaacaatt
    11761 cttgtctgcc gtgtcagatt cacttctcgg taaatctaat tttattattt acataaatgg
    11821 tcatgcggat agtgacgcag acagtagcta taattatacg ttgtcactaa atcgcagtat
    11881 tgcagtcaaa aattatttga cagaaaaagg agtaaatgat tctttgttta taattcaagc
    11941 attaggagaa gaaaatccac tggttgccaa tacaactcct attgagaaag caaaaaacag
    12001 aagagttgag ctttatgtat tatatcgcaa ttatgaaaag aaagaaatca tctatcctaa
    12061 atactacgat acagttacag attgcaacag agatacattg gtagagttgg aaagtggata
    12121 caaagtgcta ctatctgttt gtgattggaa aaaaaacaaa aaatgtctga ccataaaaaa
    12181 atctttaaac tataaattta taagtaaaga aaattggcta aaaaaacatt tagggtttaa
    12241 aaactatgaa aaaataatcc gctgtgaacc gcattataat tttcatatcc tatcctgtca
    12301 agatagttgt tttagccgac ctgctaaatt gtatattcct caatttcaag cacccggttt
    12361 gactacaagt gcaggagcat ctcagaaaaa aaataataaa aacgcaaaaa ttaaacttgt
    12421 atttaaacaa accaaaatag gtagctacgt ctactatgtt gctgatcttt attgtccggg
    12481 gatgattaat tgttacttag acaaaagatg tacgcatcct gtaagcctgt attttaaaag
    12541 aaatacacga gttttggcct attcatatac tgaaaacaat ttggagtaca acaaggatac
    12601 ggtcattcaa atcaaccctg aaaatcaaaa aaaaataacg acaaactaca gaaatgcttt
    12661 ttttaatacg atactaataa agcatcatga cgacacaata caattaaaag atattcctat
    12721 tgaactattt gcacacatca gaaaaaaaat aaactcgaat ttgggaacag aaagcaagtc
    12781 atacttcttt ttcattccgt atcgaaaaaa atacaaatgt atgcattata cgaaatataa
    12841 attaagagta agagacataa ataaattaca gcattttaat ttgcaggaat taagtggtgg
    12901 aaagtagctg tataattcat tattcatgca ttatttaaag aactagtctg tttaaaattt
    12961 taagattaca gatgtagtgt gcaatttttc aataagataa agaaattact taacctgagt
    13021 ccgggctgat taagaaaata atgtggtaaa ttaaataatt ctcatttttc ctccatagta
    13081 aaaagtagaa agaattaaaa ggagctggtt gccgtaaata tatagatact tgatgtaaat
    13141 agctgaagtt gaggtcagat tgatagtgat aatagtttta cttttgataa acaaattcct
    13201 tagatttatc tttattatct acaaaaaaaa tgaaaaacaa attaataaat ttcaatcatg
    13261 aggacttcga gatagtaagg aatattgctt tatcatttcc ctacacagaa gaaagtattt
    13321 cacacgaggg aactccttca gtaaaagttt gtggcaaatt gatgtgcaga cttcacgaat
    13381 gtggagagta tattcctata cgtcttgact ttgaaataag agaaaaatat ttagaaaatt
    13441 atccagaaat ttttcatgta cctgaacatt ttaaagcata tccatacatt tgtatgtgga
    13501 aacataacta cgacataaat ttattgatgg aaattcttga acaaagttgg aaaggacttg
    13561 caacacaaaa acaagtaaaa gaatattacg aaaggaaaaa ggaatgatta ttaattgagt
    13621 cgttacccaa ccacaccctt cttctccatt accaacctca ctcctgcttc ttacaaaact
    13681 caatagcttg acggcataaa ccgatgtctt ttataccccg cgagatctcc agtttgctgt
    13741 tcaaatctac tatttctact gatgttttct gggcgagttg tttgatgcgc ggaatatcat
    13801 ccacaccaat acctcccgat aaaaacaggg gctttatcaa actatagtgg ctgagtaaat
    13861 cccagttaaa tgccagtccg ctgccaccat gcaatgcagt tttcgtatca aaaagaaaat
    13921 aatcgcaata ctcgtcatag gcttccattg ctgaccaatc ggtgtattca tctataccga
    13981 atgcttttat cagcttaaaa t
//
LOCUS       DAOQII010000037        13750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2156_length_13750_cov_5.029865, whole genome shotgun sequence.
ACCESSION   DAOQII010000037 DAOQII010000000
VERSION     DAOQII010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13750
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2156_length_13750_cov_5.029865"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..487)
                     /locus_tag="PLF48_03060"
     CDS             complement(92..487)
                     /locus_tag="PLF48_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF48334.1"
                     /translation="MEEKISSFLDLNVWKKAHQMNLEIYKLTVNFPQNAPPALADLLV
                     RSSTAVTVCIAEGFQKRQRQEKVENYGNAQSKLTEVQYLLLLTNDLGYADTKEVQDNL
                     TEIQKMLGGLMRSVSGQFKTYKAKEEDEN"
     gene            complement(539..2014)
                     /gene="accC"
                     /locus_tag="PLF48_03065"
     CDS             complement(539..2014)
                     /gene="accC"
                     /locus_tag="PLF48_03065"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNF48335.1"
                     /translation="MKKILVANRGEIALRVMRTCKQMGIATVAVFSEADRNAPHVRFA
                     DEAVCIGPAASKESYLRMDKIIEVCKNLNVDGVHPGYGFLSENAAFSKACEDNGITFI
                     GPTPFAIETMGNKLAAKQAVSKFNVPMVPGVDHSITTAEEAKKIALQIGFPVLIKASA
                     GGGGKGMRVVYAEAELEEQMQLAVSEATSAFGDGSVFIEKFVTSPRHIEIQVLGDKHG
                     NIVYLFERECSVQRRHQKVVEEAPSAVVSPEMRRQMGEAAVKVAQSVNYTGAGTVEFI
                     VNENLDFYFLEMNTRLQVEHPVTEMITGIDLVKEQIRIAEGLPISFKQEDLTIDGHSI
                     ELRVYAEDPENKFLPDIGTLSTYKIPHGDGIRVDDGFDEGMEIPIYYDPMIAKLIVHA
                     PNRMAAIEKMKYAIEQYKIKEVKTTLPFGMFVMNHPAFISGNFDTKFVENYFHPDKKD
                     ENEAILAVALSALLMENSNQEMIIQEEGNIQQNSDWFRARQ"
     gene            complement(2140..2775)
                     /locus_tag="PLF48_03070"
     CDS             complement(2140..2775)
                     /locus_tag="PLF48_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNF48336.1"
                     /translation="MEKKIRILTTLSSIKQMLLWLFLLFVVVHPVAAKKKKSKPTKQT
                     IAKAAVKKDSITKKVVPIDILPAKDEEFASNTTSDKANQIIELAKKQLGMPYIYASAS
                     PANGGFDCSGFVYYVMTNFKYKVPRTSKDYMNFGTTIAKENAQKGDVILFTGTDASTR
                     VGGHVGIVLENTNGDITFIHSSSGKAKGVTISKLSEGYYTQRFLKVIRVVK"
     gene            2805..3251
                     /locus_tag="PLF48_03075"
     CDS             2805..3251
                     /locus_tag="PLF48_03075"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF48337.1"
                     /translation="MSIHEERNGYELSTDKSQLDTDRIHHYLSQESYWAKGIPKFIVT
                     KSIQHSWCVGIYKNKEQVAFARVVTDYATFAYICDVYVEEAHRGKGLSKWMMEVIMQY
                     RELHQIRRWTLATRDAHGLYKQFGFREPDKACNNLEIRKDDFYLNM"
     gene            3254..3907
                     /locus_tag="PLF48_03080"
     CDS             3254..3907
                     /locus_tag="PLF48_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNF48338.1"
                     /translation="MLPAIYHLIKKEIQLEWRQRTALGTILVYILGSTFLIYLVFEGT
                     TNNKTWVALFWVISLFTAINTLSRSFQKEANEQFYYWRNLVKPVSLILAKIIYNSILV
                     TVLSVLIFGVMLLFFNQKIENKSLFLPLIICGSIGYSNLFTLLSAITARTNNAVLLAI
                     LGFPIILPLLLLIIKLTGLIDFSTTDNDIYLNFGIILLLDFITLLLSIILFPYLWKD"
     gene            3916..4617
                     /locus_tag="PLF48_03085"
     CDS             3916..4617
                     /locus_tag="PLF48_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HNF48339.1"
                     /translation="MKANWWKYLGGLLILYTIIFGLLNPLPNLNILDESIRNLFYHVP
                     MWFSMMGMLFVSLIYSIKYLNGGNIQDDIKAISCAQVGFMMGILGITTGSIWARVTWG
                     AWWVFSEVKLNGAAAGLLVYAAYFILRGSFDDEEKKARYSAVYSIFAFVMFMVLINVI
                     PRITDSSLHPGNGGNPGFNTYDLDNGLRMVFYPAVIGWLLFSGWMAQLLIRIKKLYRK
                     IHKLDIEDNILKEIQ"
     gene            complement(4804..5829)
                     /locus_tag="PLF48_03090"
     CDS             complement(4804..5829)
                     /locus_tag="PLF48_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48340.1"
                     /translation="MSTPTPPVDPKSFWKKPEGKTGLLFIVGALAALGYGLFKLMPIL
                     LALATNTLALAGILMVLGALIYMILDPKVRNLVFYIYKSIMRWITGIFVQIDPIGILE
                     TYIDELKSNLSKMDKQIGLLRAQMRKLKDIIDQNQRDIQSNLTMASQAKKSEKEGLMI
                     LKTRKAGRLQDSNMRLEDLYKKMEILYRVLGKMYDSSEILLEDVIDQVQVKKQEREAI
                     RTSHSAMKSAMNILSGNNDKRYMFDQAMEAITDDVSMKIGEMERFMDLSTNFMDSVDL
                     QNGVFEEEGLKMLEKWEKEGVSLLLGKDKDLLINSTDANVVDLDAPLQKTDKSEHTNQ
                     YTDLFKM"
     gene            complement(5906..6577)
                     /locus_tag="PLF48_03095"
     CDS             complement(5906..6577)
                     /locus_tag="PLF48_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48341.1"
                     /translation="MKTNDMGIDLNKLKSLFIVTDETNKTETTDNSSNQQTTNDSTSQ
                     QVVTNTTGGQADNSIIETLFKAMSENNVQGFDYFEYKQSLKTLLGMLDESTAFKSAYA
                     TAATMGLTKQKLIDSANFYIKVLDKEKEKFTQAVDAQVAANIKGKESEIEQAKNQINE
                     KSEMIRKLTDEISALQQKMNEIQSHIIQATQKIQATSANFEASYQKIASDIKADVAKI
                     NQHIN"
     gene            complement(6829..8889)
                     /gene="fusA"
                     /locus_tag="PLF48_03100"
     CDS             complement(6829..8889)
                     /gene="fusA"
                     /locus_tag="PLF48_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNF48342.1"
                     /translation="MKNITKLRNIGIIAHVDAGKTTLTERILYYTGLIHKIGNVDDGN
                     TVMDKDPQEKKRGITISSAAISTTWKLDNTNYAINLIDTPGHIDFTIEVERSLRVLDG
                     VVALFCATSGVEPQSENVWMQSEKHQIPKLCFVNKMDRAGADFFAVLSDIQSKLQTQA
                     VAIQIPIGAEDNFIGIIDLIEFNARIWRNEGSESNYVLTEIPVELVDEAMHFRSTLLE
                     TLANHDDEFLDHYIKEESNLDAKYIHAVLRKATLSGKIQPVLCGAAYKNIGVQSLLNA
                     IVSYLPAPDDRIYTITDEQHKPVSNDTVSALVFKVINDPYVSRLTMIRLFTGSINNGD
                     FLKNQRTGEKVRISRLLKIHADKYFAVEHANAGDIVAISGLKDVRTGDTLCSLNESVL
                     LDSITIPPPVISVAIEPKQTQDVKQFGMYLAKLTDEDPSLSVTVDANTGQTLLSGMGE
                     LHLEVSIEKLKLQYGLEVSQGKPKVAYKEVFTNTVRHKERFKKQNSGSGQFAQIEFMI
                     GPSKEAESGLVFINDMKGGGVPKEFIPSVEKGFLAAMQNGVLSGYPMENLRIVLLDGE
                     AHSVDSHAYDFEIVAQKGFKSIALECRPKLLEPIMLVEIMTVDEFTGAIISDISKRRG
                     VVQSISDKRVHKLINAFIPLGAVFGYISDLRTIASGRASVNMKLSHYALLPEQLQMEV
                     LANH"
     gene            complement(9177..11081)
                     /locus_tag="PLF48_03105"
     CDS             complement(9177..11081)
                     /locus_tag="PLF48_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF48343.1"
                     /translation="MSYTSKYIEKCVDEFIESNQLKKPFYKAGEINPLKIFGISNALP
                     KMNLSHEEILFCFDKRESGLFKGFVLTDFKLHYILQDKPKVCNLEDLTSISKENVQDI
                     LSPDYTDLTDSLYALFQKIAHADSAAEKEQFFANDYKKQKPKTMKNENQILTDDFLAL
                     VLDEGNKIADILEKLNADTKFLEVLQASLTKSDSLTNEFKPNHVALQDIIFLFNKLFE
                     VANDIPIRAKFILAYLFERLQGKDMLETIEPERLEKMLSNEKFLQSVETVRKAQFLKT
                     TAEYKNQLILTSILSRVSNAQFEPLAGFYYSLATLFLNTDEELNDEEKKELDAIHQLC
                     FQPKRYIDGVRQSEVPDDDTLEKVMAEMNELIGLKNIKTDINDLINYLKVQKLRETQQ
                     LKVSDRMLHSVFMGPPGTGKTTIARLLGRIYKHLGYLKSGHLVETDRAGMVAGYVGQT
                     AIKVDEIIKAALDGVLFIDEAYALAKDDSGRDFGHEAIETLLKRMEDHRDDLVVIAAG
                     YPDEMKEFIQSNPGLQSRFNRYFNFEHYTAYEMLEIFNLNAKKADYVVTEAASKKLSL
                     IFENLYAKRNKTFGNARVVRNIFEQCIANQANRIIQLNEITKELLMTLEEADIPNVEE
                     TIKQVFVFQN"
     gene            11189..12721
                     /locus_tag="PLF48_03110"
     CDS             11189..12721
                     /locus_tag="PLF48_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNF48344.1"
                     /translation="MNKLTTFGKISITVIIIGVLFGLFQLLKKTSFFNKAVKEQTELK
                     EGKKLDSKDAIKIGVNTWGGFAGGQYFNEGFDANTNSRFTKEYGIPVEFKVIDDFDAC
                     RAAFKSGDIDLMFGTLDAFPTEVEGLKEFNPQVLFQCDWSRGGDAIVVRRGINSANDL
                     KGKTVGVAPLTPSHSFLIWMLEASNLTTKDIQLVEFPNAIDAASAFKAGKLDAAVVWS
                     PDDQACIKSLPGSKVLQSTKNASKIIADIIIGKKAYVEANKDKLAKLYEGWMIGSAEL
                     NSSEQAKNKAAKILAKGYNIPEEDALFAINNTRLTTFGDNLQFFGLDPNYKGLTGEQL
                     YTKMTSVYQKLGYAGKGVPNWRLIANPELVEEFTKKDAPNQQAEDAKNFSTPTEADKT
                     TEASSSKAVSISFRTGEYQLDDNTKYIIDKEFAELAKVFDNARIRIEGNTDNVGNPQT
                     NKTLSYKRAQAVADYLRTQYNMPENRFIIVGNGSDKPVADNSTDAGKAKNRRTDFQFI
                     NE"
     gene            12732..13529
                     /locus_tag="PLF48_03115"
     CDS             12732..13529
                     /locus_tag="PLF48_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF48345.1"
                     /translation="MYVDLRKATYPSSVKIALTVGGFVFLLIVWQVLFSLSVFPQSIL
                     PSPLQVLKAFKELYESDGLVKNMMTSIYLNFMGYFEALAIAIPLGFIIALTPPLRLLF
                     SKPIDTLRFLPLTAVTGLFIAWFGIGNSMKIHFLAFGILVYLLPVIIQRIDEVDDVYL
                     QTVYTLGASKWQTLKTIYFPSVLSKLSDDTRVLVAISWTYIIIAEMLNSTGGLGSLIF
                     KVGQRQGRIDKVFALLIVIILIGFLQDRLFVLLDKLLFPFKHKTAKA"
     gene            13526..>13750
                     /locus_tag="PLF48_03120"
     CDS             13526..>13750
                     /locus_tag="PLF48_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF48346.1"
                     /translation="MNVDTVLKNIITLKNVSQSYDDGKTFILKDVSLNIEDKPNIGQF
                     VILMGKSGCGKSTILRYIAGLQKPTSGEVLV"
BASE COUNT         4083 a         2668 c         2431 g         4568 t
ORIGIN      
        1 atggagattt tgtcatcagt gtaaccagta tgcctatacg aggaaaaggc agagctaata
       61 cgctgaaaat atctgaagtt taaaccaaga tttaattttc atcttcttct ttagccttgt
      121 atgttttaaa ttgtcctgaa acagagcgca tcaatccgcc taacattttt tgaatttcag
      181 ttaaattatc ctgcacctct ttcgtatcgg cataacccaa atcattcgtc agcaagagca
      241 aatactgcac ttctgtcaat ttagattgtg catttccgta attctccacc ttttcctgcc
      301 tttgtcgttt ctggaatcct tctgcaatac aaactgtgac agccgtggaa gaacgcacaa
      361 gcaaatcagc taaagcaggt ggggcatttt gagggaaatt tactgtcaac ttataaattt
      421 ccaaattcat ctggtgggct tttttccaaa catttaagtc gagaaaggat gaaattttct
      481 cttccattga agctatttac tttcaatata cataaaaaaa tgcatataag caagtaaatt
      541 attgccgtgc tctgaaccaa tccgagtttt gctgtatatt tccctcttct tgaataatca
      601 tttcctgatt gctgttttcc atgagcaagg cagataaagc aactgctaag atggcttcgt
      661 tttcgtcttt tttatcggga tgaaagtaat tttctacaaa ttttgtatca aaatttccgg
      721 aaataaatgc cggatgattc atgacaaaca taccgaaagg cagagttgtt tttacctctt
      781 ttattttata ctgttcaata gcatatttca tcttttcgat agcggccata cggttggggg
      841 catgtactat caacttggca atcatgggat cataataaat cggaatttcc atgccttcat
      901 caaaaccatc atccacccga atgccgtctc catgcggaat tttataagta gataatgtac
      961 cgatatcggg taaaaattta ttttccgggt cttcagcata caccctcaat tcaatactgt
     1021 gtccgtctat tgtcaaatcc tcctgcttga atgaaatggg taaaccttct gcaatgcgga
     1081 tctgctcttt aaccaaatca atgccggtaa tcatttccgt taccggatgc tccacttgca
     1141 agcgcgtatt catctccaga aaataaaaat ctaaattttc attcacgata aactccaccg
     1201 tgcctgcacc ggtatagttg acagattgtg ccactttcac agccgcctct cccatttgtc
     1261 tgcgcatttc aggtgacact actgcgctcg gagcttcttc cactaccttt tgatgccgac
     1321 gttgtacact gcattctctt tcaaataagt aaactatatt tccatgctta tcgcccaaca
     1381 cctgaatttc gatatgacga ggtgaggtaa caaatttctc aataaacaca ctgccgtcgc
     1441 caaatgccga agtagcctca gaaacagcaa gttgcatttg ctcttcgagt tcagcttcag
     1501 catatactac ccgcatgcct tttccgcctc cgcctgcaga tgcttttatc agtaccggaa
     1561 aaccaatctg caaggctatt ttttttgcct cctctgcagt agttatagaa tgatcaactc
     1621 ccggcaccat gggcacgtta aacttagata ctgcctgttt tgccgccagc ttattgccca
     1681 tagtttcaat agcaaaaggt gtgggaccga taaaggtgat gccgttgtct tcacaagctt
     1741 tggagaaagc tgcattttcg ctcagaaagc catagccggg atgaacacca tctacattca
     1801 gatttttaca cacctctata attttatcca tgcgcaagta agactcctta gatgccgcgg
     1861 gaccaataca tacagcttca tctgcaaaac gcacatgcgg ggcatttcta tctgcttcag
     1921 aaaatacggc aacggtggct atgcccatct gcttgcaagt gcgcatcaca cgcaaggcta
     1981 tttcacctct gttggctacc aaaatctttt tcatacaccg gaattgaagt gcaaaaatac
     2041 aatgtggaaa tgagaaagta aatgaaatta aaagtttagc tgatttatga ctctaatatt
     2101 taccactagt tgtcattaaa cttaaataat ccatacaatt tactttacca cccgtattac
     2161 tttcaaaaaa cgctgtgtat aatagccttc ggagagcttg ctgatggtaa cccctttcgc
     2221 tttaccagat gaggaatgta taaacgtgat atcgccattt gtattttcta agacaatacc
     2281 gacatgcccg ccaacacgag tgcttgcatc tgtgccggta aacaaaatca catctccttt
     2341 ttgggcattt tctttggcta ttgttgtacc aaagttcata taatctttgg aagtacgcgg
     2401 cactttatac ttaaaattag tcatgacata atagacaaat ccgctgcaat caaagccgcc
     2461 gttagccggc gaagcagagg cataaatata aggcatgccc aattgttttt tggctaattc
     2521 aataatttga ttagctttat ctgatgtagt attagacgca aattcctcat cctttgccgg
     2581 taaaatatca atgggtacta cttttttggt aatggaatcc ttttttactg cagctttggc
     2641 tatagtctgt ttagttggtt ttgatttttt ctttttggcg gctaccggat gtacaactac
     2701 aaacagcaag aacagccaca agagcatctg cttaattgag gataaagtcg ttaatattct
     2761 tatcttcttt tccatagccg ttaaaattaa tgtatttttg cgtaatgagc attcatgaag
     2821 aacgaaacgg ttatgaatta agcaccgaca aatcgcagtt ggacacagac cgtattcatc
     2881 attatctctc tcaagaatct tattgggcaa aaggcatacc aaaatttatc gttacaaaaa
     2941 gcattcagca ctcctggtgt gtgggcatct ataaaaacaa agaacaggtg gcatttgcca
     3001 gagtcgtaac agactatgct acatttgcct atatctgcga tgtgtatgtg gaggaagctc
     3061 atcggggaaa aggattgtca aagtggatga tggaagttat tatgcaatac cgtgagctgc
     3121 atcagatacg aagatggaca ttagccacac gtgatgcgca tggtttatac aagcaattcg
     3181 gtttccgcga gccggataaa gcgtgtaaca atcttgaaat ccgcaaggac gatttttatt
     3241 tgaatatgta agtatgttgc ctgccattta ccatttaata aaaaaagaaa tacagttaga
     3301 gtggcggcaa cgcacggctt taggcactat tttagtctat attctgggca gtaccttttt
     3361 aatttattta gtttttgagg gcacgacgaa taacaaaacc tgggtggcat tgttttgggt
     3421 aatcagtttg tttaccgcta tcaacacgct atctcgatca ttccagaaag aagccaatga
     3481 gcaattttat tactggcgca atttagtgaa acccgtgtcg ctgattctgg caaaaattat
     3541 ctacaacagc atattggtta ccgtactgag tgttttaatt tttggcgtga tgttgttgtt
     3601 ttttaatcaa aaaatagaaa acaaatcact ttttctgccc ttaatcattt gcggctccat
     3661 cgggtattca aatttattta ctttactttc agccataacg gcacgcacca ataatgccgt
     3721 gctattagcc attttggggt ttcccattat tttgcctctt ttgcttttaa tcataaaact
     3781 aacaggctta attgattttt ctaccacaga taatgacatt tatctcaact tcggcattat
     3841 ccttttattg gattttatta cccttttact ctcaataatt ttgtttcctt atctttggaa
     3901 agattaatta ctgacatgaa agcaaattgg tggaaatatt taggcggatt gctcatactt
     3961 tatacgatta tattcgggtt gcttaatccg ttacccaact taaatatctt agacgaatcc
     4021 atacgcaatt tattttatca cgtacccatg tggttttcga tgatgggcat gttgtttgta
     4081 tcgcttattt attctattaa atatttaaac gggggcaata ttcaagatga tattaaagcc
     4141 atctcttgtg cacaggttgg ttttatgatg gggattctag gcattacaac ggggtctatc
     4201 tgggcgcgtg taacgtgggg agcctggtgg gttttttctg aagtaaaact gaatggtgct
     4261 gctgcgggat tattagttta tgccgcatat ttcatcttgc gcggttcatt tgacgatgaa
     4321 gaaaagaaag cgcgctacag tgctgtttat tccatttttg ccttcgtgat gtttatggta
     4381 ttgattaatg tcattccacg catcactgat tcttctttgc atccgggcaa tggtggcaat
     4441 cccggattta atacctacga cttagataat ggattgcgaa tggtctttta tcctgcggtt
     4501 atcggttggt tattgttttc cggatggatg gcgcagttgc tcattcgtat caaaaagttg
     4561 tatcgcaaaa tacataagct ggatatagaa gacaatatcc tgaaagagat tcaataattc
     4621 aacgtagtaa gcttagttct ggataagcgt ttcaattaaa aaatagttga ttttgtatat
     4681 agaaccacct ccttttaatt cctcctcctt gttaaggtgg agtaaaaaat gagaaatcaa
     4741 ctattttttt tcgccttaat tttcttaacc agaactaagg ttagtaaact taattacatg
     4801 ctcttacatc ttgaacaaat ccgtatattg attagtatgt tctgatttat ctgttttttg
     4861 taaaggcgca tctaaatcaa ctacgttagc atctgtactg ttaatcagta aatctttgtc
     4921 tttgcctaac aataatgaaa cgccttcttt ttcccatttt tccaacattt tcaatccttc
     4981 ttcttcaaag acaccatttt gcaaatctac cgaatccatg aagttggttg ataaatccat
     5041 gaagcgttcc atctcgccta ttttcatgct tacatcatcc gtgatggctt ccattgcctg
     5101 atcaaacata tagcgtttgt cgttattgcc actcaggata ttcatggcag atttcatggc
     5161 agaatgagaa gtgcgtatag cttcgcgttc ttgttttttt acctgcacct ggtcaatcac
     5221 atcttccagt aatatctcag agctgtcata cattttgccc agcactctgt agagaatttc
     5281 catttttttg tacaaatctt ccaagcgcat attggaatct tgcaggcgac cggcttttct
     5341 ggttttcaaa atcatcaatc cttctttttc gctttttttc gcttgcgaag ccattgtcag
     5401 attgctttgt atatcgcgtt gattttggtc gatgatatct ttgagtttgc gcatttgtgc
     5461 tctgagtaat ccgatttgtt tatccatttt agacaagttg gatttcagtt catcaatata
     5521 tgtttccagt atgccgatag ggtcaatctg aacgaatatc cctgttatcc aacgcatgat
     5581 ggatttgtaa atatagaaga ccaaattgcg tactttagga tccagaatca tataaataag
     5641 tgcacccaaa accattaaaa ttccggccaa agccaaggta ttggtagcca gcgccaagag
     5701 gattggcatt aatttgaata aaccgtaacc taaagctgcc agtgcgccta cgatgaataa
     5761 tagtcctgtt ttgccttccg gttttttcca aaacgatttc ggatctacgg gtggagttgg
     5821 agttgacatg atgaaatttt agattttaaa ttttagttta tggcctatat tattactaat
     5881 tactttttac ttatcactaa atcctttagt ttatatgctg atttattttt gctacatctg
     5941 ctttaatatc agaagcaatt ttttggtaag atgcttcaaa attggcagaa gtagcttgta
     6001 tcttttgagt agcttgaatg atatgcgact gtatctcatt catcttttgt tgcaaggctg
     6061 aaatttcatc tgtcagttta cgaatcattt cagatttttc gttgatttga tttttagctt
     6121 gttcaatttc tgattccttg cctttgatat tagctgcaac ttgtgcatct acagcttgtg
     6181 taaatttttc tttttctttg tctaatactt taatataaaa attggcagag tctatcagtt
     6241 tttgtttggt aagacccatc gttgccgcag tggcgtatgc cgatttgaaa gcagtggatt
     6301 cgtccagcat ccctaacaag gtcttcagtg attgtttgta ttcaaaataa tcaaaaccct
     6361 gaacattgtt ttccgacata gctttaaaca aggtttctat tattgaatta tctgcttgtc
     6421 cgccagtggt atttgttacc acctgctgac ttgttgaatc attcgtagtt tgttgattag
     6481 atgagttatc tgttgtttca gttttattcg tttcgtccgt tacgataaac agtgatttca
     6541 atttattcaa atcaattccc atatcattcg ttttcacaaa aatagcattt acaatacatt
     6601 aaatgcaaaa atggataaaa aagttccgaa accggaagaa ttgaagaaac agaaagagta
     6661 gcgggcagtt agcagaacgc agcaggcaat gtataaaatg tgaaaagcaa gcttaaaact
     6721 attaaatatg aatacccgaa tgctctttaa caaacaaaaa cctgactgct gtaataaaaa
     6781 aggatatcgg ggcgcggagc gccccgaaaa tcccgcataa tccaaaaatc agtgattcgc
     6841 caatacttcc atttgcaatt gttccggcaa tagtgcataa tgagaaagtt tcatatttac
     6901 agatgctctt ccacttgcaa ttgtacgcaa atcagatata tagccaaaaa cagcacctaa
     6961 gggaataaaa gcatttataa gtttatgaac ccttttatca gaaatggatt gtactacccc
     7021 ccttcgttta ctaatatctg aaataattgc gcctgtaaat tcatcaaccg tcataatttc
     7081 aacaagcata atcggctcca acaattttgg tcgacattcc aatgcaatag atttaaatcc
     7141 tttttgtgcc acaatttcaa aatcgtatgc atgagaatct acactatgag cttcaccgtc
     7201 aagtaataca atgcgcaaat tttccatcgg gtatccactc aacaccccat tttgcatagc
     7261 cgccaaaaat cctttttcta cagatggtat aaactctttt ggaactccgc cacctttcat
     7321 atcattaata aatacgagtc cactttcagc ttccttactt ggtccaatca taaattcaat
     7381 ttgagcaaat tgaccactgc cgctgttttg cttcttaaat cgttctttat gtcttaccgt
     7441 attcgtgaat acttccttat aagcaacttt tggtttgcct tgtgaaactt caagtccata
     7501 ctgtaacttc agtttttcga tgcttacttc aaggtgaagt tctcccatgc cacttaataa
     7561 agtctgacct gtattggcgt ctaccgttac ggagagagac ggatcttcat ccgttaattt
     7621 agccaaatac attccaaatt gtttaacatc ttgagtttgc ttcggttcaa tagccacact
     7681 aattacaggt ggtggaatcg ttatactatc caataaaaca gattcattta aactgcataa
     7741 ggtatctcct gttcgcacat cctttaagcc tgaaattgca acaatatcac ctgcatttgc
     7801 atgttctact gcaaaatact tatcagcatg aatcttgagc aacctgctaa ttcttacttt
     7861 ctcccctgtt cgctgatttt tcaaaaaatc gccattatta atactacctg taaacaaacg
     7921 tatcatggta agtcggctta cataaggatc attaattact ttaaaaacca gcgctgacac
     7981 agtatcatta ctgacaggct tatgctgttc atcggtaatt gtatatatcc tatcgtccgg
     8041 tgctggcaag tagctcacta tggcatttaa taatgattgt acaccaatat ttttgtaagc
     8101 tgctccgcac aaaacgggtt gtattttacc tgataatgtt gcttttctca atacagcatg
     8161 aatgtatttt gcatctaaat ttgattcctc tttaatataa tggtctaaga attcatcatc
     8221 atgatttgct agagtttcta acaaagtact tctgaaatgc atagcctcat ccactaattc
     8281 aacaggaatt tctgtcagca cataattact ctccgaccct tcattacgcc aaatccgtgc
     8341 attaaattca attaaatcta tgatgccaat aaaattatcc tcagcaccga taggaatttg
     8401 tatagctacc gcttgtgttt gtaatttaga ttgaatatca ctcagcacgg caaaaaaatc
     8461 tgctccggct ctatccattt tatttacaaa acacaacttt ggaatctgat gtttttctga
     8521 ttgcatccat acattttcag attgaggttc tacgcctgaa gtagcacaaa acaaagcaac
     8581 aacaccatct aaaaccctta gtgaccgttc tacctctatt gtaaaatcta tatgtccggg
     8641 tgtgtcaatg aggttgatag catagttagt attatctaat ttccaagttg tagaaatagc
     8701 cgcagaagat atggtaatcc ctcttttttt ctcttgcgga tctttgtcca ttacggtatt
     8761 tccatcatca acatttccta ttttatgaat taatccggta tagtataata tcctttcggt
     8821 taaagttgtt ttccctgcat cgacatgtgc aataattcca atatttctaa gttttgtaat
     8881 gtttttcatc ttgttcattt ttaaaagtat aaaataaatt tctctgctta acactaaggt
     8941 gttaagatta tttttatttc agtaacataa aaaatgcctt tccgaaaaga aaggcattat
     9001 aattttttgt atgcttattt ttttcagaaa agaataatta tgctaccaga aaacactgct
     9061 ctgattgctc catttattgc tataatattt ttattgacgc gaaacatctg aaatattttg
     9121 tgcaaagata attcaacttt tcaataatca aagctgatct ttattttttt atttaattaa
     9181 ttctgaaaca caaaaacttg ttttatcgtt tcttctacat taggaatatc tgcttcttct
     9241 aaagtcatca atagctcttt cgtgatttcg ttcagctgga taatgcggtt ggcttggttg
     9301 gcaatgcatt gctcaaaaat atttcgaaca acacgggcat ttccaaaggt tttattgcgc
     9361 ttggcatata aattttcaaa aatgagggaa agcttttttg atgcggcttc tgttactaca
     9421 taatctgctt ttttggcatt caggttaaat atttccagca tctcgtaagc tgtataatgt
     9481 tcaaaattaa aatagcggtt aaagcgcgac tgcaagcccg gattggattg aataaattcc
     9541 ttcatttcgt cggggtagcc ggcagcaatg acgaccaaat catcgcgatg atcttccatg
     9601 cgcttgagca atgtttctat agcttcatgg ccgaaatctc tgccactgtc gtcttttgcg
     9661 agggcatagg cttcgtctat gaacaacaca ccatctaatg ccgctttgat gatttcatcc
     9721 actttgatgg ctgtttgtcc aacatatccc gctaccattc ccgcccggtc tgtttctact
     9781 aaatgaccgc ttttgagata acctagatgt ttataaattc tgcctagcaa acgggctatt
     9841 gttgtttttc ctgttccggg aggacccata aaaacggaat gtaacatacg gtcagatact
     9901 ttcaattgtt gagtctcacg caacttttgt actttcagat aattgattaa atcgttgata
     9961 tctgttttaa tatttttcag cccaataagc tcattcattt ctgccattac tttttctaag
    10021 gtatcatcat cgggcacttc actttgcctt acgccatcaa tatatcgttt aggctgaaaa
    10081 caaagttgat gtatggcatc caattctttt ttttcttcat cattaagttc ttcgtctgta
    10141 ttgagaaaca aagtagcaag agaataataa aatccggcta agggttcaaa ttgtgcattg
    10201 gacacacggc taagtatgga agtgagaata agctgatttt tatattcagc agtagttttt
    10261 agaaactgcg ctttacgaac tgtttctacg ctttgtaaaa atttttcgtt ggataacatc
    10321 ttttcgagtc gttccggttc tattgtttcc agcatatcct tgccctgcaa gcgttcaaac
    10381 aaataagcca gaataaattt tgctcttatc ggaatatcat ttgcaacttc gaataattta
    10441 ttaaaaagga aaataatatc ctgcaatgcg acatgattgg gtttaaactc attcgttaag
    10501 gaatccgatt tggttaatga agcctgcagc acttctaaaa atttagtatc cgcattgagt
    10561 ttctccaaaa tatcagcaat cttattgcct tcatctaaaa ccaatgccaa aaaatcatct
    10621 gtcaggattt gattttcatt tttcatcgtt ttgggttttt gttttttata atcatttgca
    10681 aaaaactgct ctttttcggc ggcactgtct gcatgggcaa tcttttgaaa taacgcatat
    10741 aaggaatcag ttaagtcagt ataatcaggc gataagatat cctgaacatt ttcttttgaa
    10801 atagatgtta aatcctccag attgcatacc ttcggtttat cttgcaatat ataatgcaac
    10861 ttaaaatcgg tgagtacaaa tcctttaaac aaaccacttt cgcgtttatc aaaacaaaac
    10921 aatatttcct catgcgacaa attcatttta ggcaaagcat tggagatgcc gaaaattttt
    10981 agcggattga tttcacctgc tttgtaaaaa ggctttttta gttgattact ttcaataaat
    11041 tcatctacac acttttcaat atattttgat gtataactca taaatttaac tttacaggaa
    11101 ctttttaaga acgcttttcg tttaaaattc gttccttata agaacaatca actaaaataa
    11161 ttaaatttgc taaaataaaa cacttgttat gaacaaactc accacatttg gaaaaatatc
    11221 cattactgtc attatcatcg gagtactttt tggattattt caactcttaa agaaaacaag
    11281 cttttttaat aaagcagtga aagaacaaac tgaactcaaa gaagggaaaa aattagacag
    11341 taaagatgct ataaagatag gtgtgaatac atggggaggt tttgcaggag gacaatattt
    11401 caatgaagga tttgatgcga atactaattc cagatttacc aaggaatatg gcattcctgt
    11461 tgaatttaaa gtaattgatg atttcgatgc atgccgtgct gcattcaaat caggagatat
    11521 agatttgatg tttggaacat tagatgcatt ccccactgag gtagaaggct taaaagaatt
    11581 taacccacag gtattattcc agtgtgactg gtcaagaggt ggcgatgcta ttgtagttcg
    11641 tcgcggaata aactctgcca atgacttaaa aggaaaaact gtcggtgttg ctccattaac
    11701 tccatcgcac agctttttaa tctggatgct ggaagccagt aacctgacaa ctaaagatat
    11761 tcagttggtg gaatttccaa atgccattga tgccgcttct gcttttaaag ccggcaaatt
    11821 agatgctgcc gtagtatgga gcccggatga ccaagcatgt ataaaatctc tgcctggttc
    11881 aaaagtatta cagagtacta aaaatgccag caaaattatt gcagatatta ttatcggaaa
    11941 aaaagcatat gtagaagcta ataaagataa attagccaaa ttgtacgaag gctggatgat
    12001 tggctccgca gaactaaaca gtagcgagca agcgaaaaac aaagcagcaa aaatcttagc
    12061 aaaagggtat aatattcctg aagaagatgc attatttgcc attaacaata cacggctgac
    12121 tacgtttggc gacaatctgc aattttttgg attagatccg aattacaaag gtctgacagg
    12181 agaacaatta tatacaaaga tgacttctgt ttatcagaag ttgggatatg ccggaaaggg
    12241 cgtgcccaat tggcgactga tagccaatcc ggaattagta gaagaattta ccaaaaaaga
    12301 tgctccgaac caacaagctg aagatgcaaa aaatttcagt actcctacag aagcagacaa
    12361 aaccactgag gcttcatcaa gtaaagcggt atctatttct ttcagaacgg gagaatatca
    12421 attggatgat aatactaaat acattatcga taaagagttt gctgagctgg ctaaagtatt
    12481 tgacaatgcc cgtattcgta ttgaaggcaa taccgacaat gtaggaaatc ctcaaacaaa
    12541 taaaacgcta tcgtacaaac gtgcgcaagc tgttgctgat tatttgcgta ctcaatacaa
    12601 tatgcctgaa aacagattta tcatcgtagg gaatggttct gataagcctg tggctgacaa
    12661 cagtaccgat gccggcaaag ccaaaaacag acgtaccgat tttcagttta taaacgaata
    12721 aactcatatt tatgtatgtt gatttaagaa aagcaactta tccgtcttct gtaaaaatcg
    12781 cacttacggt tggtgggttt gtatttctct taattgtttg gcaagttttg ttcagcctct
    12841 cggtttttcc gcagagtatc ttgccttcac ccttacaggt actaaaagct tttaaagaat
    12901 tatatgaaag tgatgggctt gtgaaaaata tgatgacttc catttatctc aatttcatgg
    12961 gatatttcga agcattagct atagctattc cactgggttt tatcattgcg cttactcctc
    13021 ccttgcgctt attgttcagc aaaccaatag atactttacg ctttttgccg ttaaccgcag
    13081 taacagggtt gtttattgcc tggtttggaa ttggcaattc catgaaaata cattttctgg
    13141 cttttggtat tttagtttat ttacttcctg tcattataca gcgcattgat gaagtagatg
    13201 atgtatattt gcaaaccgta tatacacttg gtgcatccaa atggcagacg ttgaaaacta
    13261 tttattttcc cagtgtatta tccaaattat ccgatgacac acgtgtattg gtggccattt
    13321 catggacata tatcatcatt gctgaaatgc tgaacagtac gggaggtttg ggttcattaa
    13381 tttttaaagt aggtcaaagg caagggcgaa tcgataaagt atttgcattg ttaatcgtga
    13441 ttattttaat cgggtttctt caagaccgtt tatttgtctt attagataaa ttgctttttc
    13501 cattcaaaca taaaacagcc aaagcatgaa tgtagatact gtattaaaaa acatcatcac
    13561 actcaaaaat gtttcgcaat cttatgatga cgggaaaaca tttatcctca aggatgtaag
    13621 tctgaatatt gaagacaagc caaatatcgg gcagtttgtt attttgatgg gaaaatcagg
    13681 ctgtggcaaa tctacaattc ttcgttatat agcaggcttg caaaagccta catccggtga
    13741 agtgctagtg
//
LOCUS       DAOQII010000038        13737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2158_length_13737_cov_4.900672, whole genome shotgun sequence.
ACCESSION   DAOQII010000038 DAOQII010000000
VERSION     DAOQII010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13737
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_13737_cov_4.900672"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PLF48_03125"
     CDS             complement(<1..1718)
                     /locus_tag="PLF48_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HNF48347.1"
                     /translation="MITRLKSGDSRSIHLNALPGNFARLDVFDFRNIQQSLHLDFLNE
                     LLTKENFSFKITMDFSKIPKENEEQIKQLNKTTKRLNHLYYQEKEAFEQFGYSSFGFG
                     YPLLIFRDAENPKKIIKAPLFIWYLELKKDTTQSSTWRLHKSDEQAVIFNEVLQSYLE
                     KNYHVKTDDLTDLVEEDFFTERKLINCCELLLQRLNGQFDAESAIPTVLPCTNKESLE
                     LLTKQTPWLRWSGVLGMYKTQKQSIINDLEQLTSTEIKGDTVIQKEKSQAEVYPVLSI
                     DASQEEVIRSTFTQDKIIIQGPPGTGKSQTLTSIIIQALLQDKKTLMVCEKRTALEVI
                     QHLLQNTGLSHLTMLIDDVYADRNKVIEAVRQITDTEPVTFRFKEHDYKQLAGKIKEL
                     RHEINTKIKAPQTELLNNDTFEEIVLELIENSKDEKLKQQAELLAKTLRSSDYTLDSE
                     TYNTLLNIVEQAELLANQRHKEDVVFDKLLPENFIHKTPEQISIELHELATQLQLLYD
                     TTELYLVNGKKYFDEQQGIYPVLLNIFAVFSSKYRLIRTRKEQTKLEYLNAINKIKSY
                     GLCTTD"
     gene            complement(1850..2698)
                     /locus_tag="PLF48_03130"
     CDS             complement(1850..2698)
                     /locus_tag="PLF48_03130"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNF48348.1"
                     /translation="MKNTKLEWTSNDGLRIFGQQWEANSGEPKAVICLVHGFGEHSSR
                     YEHVADFFTKNGYAMIAYDHRGHGRSEGKKGHFPSYDEFLNDVGNLLKQADQNFADIP
                     KFLYGHSMGGNVVANYAIKRKPDVKGVILSSPFFNTAFEPPAIKIAVGKFMRNLIPTL
                     SLPSGLDATQISRDKDVVEKYKKDPMVFDIISSKMGIELIEYGEDALAHAKDLKLPTL
                     LFHGTADGLTSFEKSKTFAQKAGSNLKFIAYEGLYHETHNEPEKEMVLTNVLKWCNEQ
                     LDNGNK"
     gene            2767..3858
                     /locus_tag="PLF48_03135"
     CDS             2767..3858
                     /locus_tag="PLF48_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF48349.1"
                     /translation="MKKSSMLLLKLSGIFILLVIIYLILVIIYAQTNNYDPEPKSKAE
                     DINIYTNPIQQDTFVFYTWNIGYCGLGAESSFFFDSPNPIKQFVYPFEVHVRPSSQTV
                     QKNIMGVLSTVRDWASDADFIALQEVDIDSRRSYGNNELRAVSQVLTNYNFSFGKNYD
                     VNFVPFPLLKPMGGVLGGIATFSRFSFQQTPTRYSFPGSFPWPKKLFFLDRCFVEHRF
                     NVKNGKQLVLLNTHNSAFDGGVLKVQEMAYLKQYLLKEYEKGNYVVVGGDWNQIPPGY
                     QPKSKESGYEELPIAIDFLPSDWKFAFDKNTPTNRKVDEPYDAASTYTTVIDYFLLSP
                     NIELTHVNGIADGFAYSDHQPVRMSFRLK"
     gene            complement(4447..5136)
                     /locus_tag="PLF48_03140"
     CDS             complement(4447..5136)
                     /locus_tag="PLF48_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48350.1"
                     /translation="MKYISFNINLFYLLFQLAFTTSCVSQDKYNYWVNLNYSKCLQSK
                     LPCECINSTQSFIFIITTNKQASRFWGISNSDPEYGEYYLYKKGTNRYYIKSINLQEM
                     SKFGFFLISNDTLENIHENNRKEFFTKYYTSDSIDFSTYAKQNIALIDTALKNKGYKS
                     LQDITMENNIGCECNFELGEINFISTKGLLKYWILENDSSVLNLYELSGTTNKCYPMK
                     LDKKLIKSFKL"
     gene            5560..5895
                     /locus_tag="PLF48_03145"
     CDS             5560..5895
                     /locus_tag="PLF48_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF48351.1"
                     /translation="MGTTKTHQYTKKQNELAIMLKAIAHPARIAIIELLLKKNTCICN
                     DIVDEIQLAQPTVSQHLKELKQAGWINGEIEGTAICYCVNPKIPQSLLHYFDTMYQKS
                     LLNTDKCCK"
     gene            5903..6937
                     /gene="arsB"
                     /locus_tag="PLF48_03150"
     CDS             5903..6937
                     /gene="arsB"
                     /locus_tag="PLF48_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNF48352.1"
                     /translation="MQTKLKFLDRFLTLWIFLAMFAGILLGYIWPDFSKNIERLSIGT
                     TNLPIAVGLILMMYPPLAKVNYSLLPQAFKETKILSLSLVLNWIVGPILMFLLAIIFL
                     RNEPGYMSGLILIGIARCIAMVIVWNDLAGGNREYAALLVALNSIFQMLTYGFYAWLF
                     INFLPHYFGIANFNIHVPMSEVAKSVAIYLGIPFFAGISSRYLLIKNKGEEWYNRKFI
                     PKISPVTLYALLFTIVIMFSLKGDKIIQLPFEVIKIALPLVTYFTIMFFISFMLGKFL
                     HIPYDKNASVSFTATGNNFELAIAVSIALFGIHSAQAFTGVIGPLVEVPVLILLVRAS
                     KYLQTKFYTK"
     gene            6934..7359
                     /locus_tag="PLF48_03155"
     CDS             6934..7359
                     /locus_tag="PLF48_03155"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNF48353.1"
                     /translation="MKILILCTGNSCRSQMAHGILQSLSEKITVCSAGTHPAIAVHPN
                     AILVMHELGLDISYHQPVAVEAYLNETWDYVITVCGGANENCPAFIGNVKNRLHIGFE
                     DPADATGSKEEVLDTFRKIRNEILVDFFAFYNTMVKKHT"
     gene            7405..8625
                     /locus_tag="PLF48_03160"
     CDS             7405..8625
                     /locus_tag="PLF48_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNF48354.1"
                     /translation="MNIQSEIQTLRKVVIHEPDNGIDNISPELAEKLLYDDIVFLPRM
                     IEEHYQFTQTLRQLIGTENVLEFGQLLQDVLRNDAIREEVIEYLFEKEDLSLGKYEKL
                     KQVPVELLASVLITGMDPETKDTLLPPLPNLVFTRDIGCVINDYVVLCKANKDARLRE
                     NFLTKYILKYHPEFACFKNKIVDFVLDENLGKDAGISIEGGDIMLVHPRHLFIGESER
                     TSLDAIFQLKQFLFENDVVDYVSVVEIPDERYCMHLDTVFTLLDKDTCVGFSPLMFEK
                     NDKVDIVTYSKDNKRAALHPSLKELILEIYPGIRCIPCGNGLSPFDRREQWTDGCNFV
                     TVKPGVAYSYERNIRTLNALKDNGFSVISSKHILAEEYTLAELDKTIITLSSAELSRA
                     RGGPHCMTFPISRN"
     gene            complement(8622..9278)
                     /locus_tag="PLF48_03165"
     CDS             complement(8622..9278)
                     /locus_tag="PLF48_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF48355.1"
                     /translation="MSNLYYPNFEPKVQFERPNLPSAKECFEYLQQFLANFDFITTND
                     VSNLYDYFEYFHYPKNEIILSKGEVCTDLYFIVKGSLIKYVEDDLGKNIILFITETNF
                     CAAVKSLHFQLPSNYYIATSENVFGLKLSYQNYFRLVADKPDFMNFFQLMIDETIDLL
                     IARIRVLLALDAKQRYEVFLNQYPDVYQRFNLQDISNFLGITPETLSRLRNTKKTTKS
                     "
     gene            complement(9439..10236)
                     /locus_tag="PLF48_03170"
     CDS             complement(9439..10236)
                     /locus_tag="PLF48_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF48356.1"
                     /translation="MAKNSKSTKHEFLEKFPNKVGSFGYDPWGFNINGIKPFVSAGKF
                     LFDKYFRVETHGMENIPAHGRVLIIANHSGQLPIDATLLGYALVTNKYAPRAPKGMYE
                     RFVPQVPFISMYFNQWGGTLGDPENCIRMLENEEAVIVFPEGAKGISKPYSKKYQLQR
                     FGLGFMYMALQTNTPIIPVGIVGCEESIINFGNVDWLEKIIKFPAAPLLLPVVFPTKV
                     VMNFGEPILFSGDTSREYLVAEKVDMVKDKIKDLIAEGLKQRKGIFS"
     gene            10371..10811
                     /locus_tag="PLF48_03175"
     CDS             10371..10811
                     /locus_tag="PLF48_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48357.1"
                     /translation="MKSFSKIGILVSLFVFLTAALGVPVIEHVCYSSNTTTIALFSDT
                     CEKDIQKNSCCKEKQKSTSKKCCENKYSYHRLVYQGQQENNFQLKFSKKITAQPDFFC
                     FYQAISKSQILFSFRVLPLPDKIRAQTISGLLCTNPELLQIFRC"
     gene            10857..13067
                     /locus_tag="PLF48_03180"
     CDS             10857..13067
                     /locus_tag="PLF48_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF48358.1"
                     /translation="MKKYYTYFWCLLSSSLYIPLYAQQSIIKIRVFDAATQQALESAN
                     VYWRDTTIGTSTDTNGLALLPRIPNQTFMLVDYLGYKTDTISINQKIGYYTAYLQPSV
                     LLNTVEINAKKQSNFISSLETKKTEKITLDELKKAACCNLSESFQTNASIDVNYQNAV
                     TGAKEIKLLGLSGLYVQNLIEGLPYMRRYSYAFGIDQLPSPWINAISISKGIPSVKNG
                     YEGTTGSMSVQLKNGFNDKNKLYFDLFGNSDSRIETNLIIHHKINTHLSTSLMFNGAF
                     RPMNMDRNEDGFLDNPKVKQYNFLNSWNIHQPDKRVEGQYYLKAISENRQSGQTDAHV
                     QHQTGTLPLYAVSIQTKRFEAFAKTGFLLKKHDASIGTQFSGVYHQQESQYGSINYNV
                     RQASFTGNLLYQTDIKNEDHMIVTGLSFTYDNLRQQLDSTRLFTSLQLPGIFAEYTYK
                     HGERISFIAAMRLDYSKQTSLQYAPRVHARFDLDKKQNTTLRLSAGRGYRVADILNEN
                     QFLLASSRTFVYQGNPITEAAWNYGFSLHQKYKLKKREGSFTVDFFRTDFTAQNTIDI
                     ENENGITTLYPKQGKSFSNSLMAEWNMEIAKGWDIKLAYKWEDVRMTYHHLLMQKALH
                     FIHRGLFSTSYKIPNNKAQFDLIMPIYGKSRMPNSFAESTINKFSNPYILLNFQILTK
                     FKIGEFFAGVENMANFKQKHPILGSPGTSYFDTYQVYAPVSGVTGYGGFRIFIN"
     gene            13092..13280
                     /locus_tag="PLF48_03185"
     CDS             13092..13280
                     /locus_tag="PLF48_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48359.1"
                     /translation="MKAIKLLSVLVIMLITASLVFTSCHGKHCKEEGKCKKEKCEKEG
                     MSDSTKSSCCKKDSTIAQ"
     gene            13299..13625
                     /locus_tag="PLF48_03190"
     CDS             13299..13625
                     /locus_tag="PLF48_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone"
                     /protein_id="HNF48360.1"
                     /translation="MKNILLMLPFLFAVISTQAKEKAEQLQFKVNGNCDMCKARIEKA
                     AKVNGVKSAEWNEQTHFIIVKFMPSKTNQTAIQQHIAAAGYDTEDVIASDDAYQALPQ
                     CCQYTR"
BASE COUNT         4342 a         2484 c         2442 g         4469 t
ORIGIN      
        1 aaatcagttg tacataatcc atacgatttt atcttgttta ttgcgtttaa atattctagc
       61 ttggtctgtt cttttctcgt gcggataagt cggtattttg aagaaaagac tgcaaaaata
      121 ttcagcaaaa caggataaat gccctgctgt tcgtcaaaat atttttttcc gtttactaag
      181 tacagttctg tcgtgtcata aagtaattgc agttgtgttg ccagctcatg tagttctata
      241 ctgatttgct ccggtgtttt atggataaaa ttttcaggaa gtaatttatc aaaaacaaca
      301 tcttctttat gtcgttgatt agccagcaac tcagcctgct ccacaatatt caacagcgtg
      361 ttgtatgttt cgctgtctag tgtataatca ctgcttcgca gggttttagc cagcaattcg
      421 gcttgttgtt ttagcttttc atcttttgaa ttttcaatga gctccaatac aatttcttca
      481 aacgtatcat tattcagcaa ctccgtttgc ggtgctttta ttttagtatt gatttcgtgg
      541 cgcagctctt ttatttttcc ggcaagctgc ttgtaatcgt gttctttaaa gcgaaatgtc
      601 acaggctccg tatctgtaat ttgccgaacg gcttcgatga ctttattgcg gtcggcatac
      661 acatcatcta tcagcatggt aagatgcgac aagcctgtat tttgaagtaa atgctgaata
      721 acttccaatg cagttctttt ctcacacacc atcagcgttt ttttatcctg caacaaagcc
      781 tgaataatta tggaagtaag tgtctggctt tttccggtac cgggtggccc ctgaatgata
      841 attttatctt gagtaaacgt actgcgtatg acttcttcct gcgatgcatc tatcgataaa
      901 acgggataaa cttctgcttg acttttctcc ttttgtataa cagtatcgcc tttgatttcc
      961 gttgatgtga gttgttccaa atcattgata atgctttgtt tttgcgtttt atacatcccc
     1021 aacacaccgc tccatcgcag ccatggcgtt tgtttggtta agagttccag cgattcttta
     1081 ttggtgcaag gcaatacagt cggtatggca ctttctgcat caaactggcc atttaagcgt
     1141 tgtaataaaa gctcgcaaca attgattaat tttctttcag taaaaaaatc ttcttcgacc
     1201 aaatcggtga gatcatccgt tttgacatga taattttttt cgagataaga ctgtaaaact
     1261 tcgttgaaaa tgacggcttg ttcatccgat ttatgcaacc gccaagtact ggattgtgtg
     1321 gtatctttct taagttcgag ataccaaata aagagcggtg ctttgataat tttcttaggg
     1381 ttttccgcat cgcggaaaat cagcaaagga taaccaaaac cgaatgagga atagccaaat
     1441 tgttcaaaag cttctttttc ctgatagtat aaatggttga gtcttttggt agtcttattc
     1501 agttgtttga tttgttcttc gttttctttc ggtattttgg aaaagtccat tgtgatttta
     1561 aatgaaaaat tttcttttgt caagagttca tttaaaaaat cgagatgcag actttgctga
     1621 atatttcgaa aatcgaatac atccagacgg gcaaaattgc cggggagagc attcaaatga
     1681 atactgcgag agtcgccgga tttgaggcga gtaatcaatt tatcgataaa ggaagaggac
     1741 actttttatt tcattttcag attgtaacgg acatggtgca acatatttta ttcagtttat
     1801 ttatgtgcag atgatttatg atgattaaac aacaattttg ccaataaaat tatttgttgc
     1861 cattgtctaa ttgttcatta caccatttaa gcacattagt aagcaccatt tccttttcag
     1921 gttcattatg tgtttcgtga taaaggcctt cataagcaat aaacttcaga ttgctgcctg
     1981 ccttttgtgc aaatgttttt gatttttcaa aggaagtcaa gccatcggca gtgccgtgaa
     2041 acaataatgt cggcagtttc aaatctttgg catgggcaag tgcatcttca ccatattcta
     2101 tcagctcaat tcccatttta gatgaaatga tatcaaagac catcggatct tttttatact
     2161 tttctaccac gtctttatct cttgatattt gtgtggcatc caatccggaa ggtaaagaca
     2221 aggtaggaat cagattgcgc ataaatttgc ctacagcaat tttgatggcc ggcggttcga
     2281 atgcagtatt aaaaaatggc gaagaaagaa tgactccttt cacatccggt ttgcgcttga
     2341 tggcataatt tgccactaca ttgcctccca tactatgacc gtataaaaat tttggtatgt
     2401 ccgcaaaatt ttggtcggcc tgttttaata aattacctac atcattcaaa aattcatcat
     2461 agctgggaaa atgccctttt ttgccttcag aacgtccatg acctctgtga tcataggcta
     2521 tcatagcata gccgtttttt gtaaaaaaat ccgccacgtg ctcgtatctc gaactatgtt
     2581 cgccaaaacc gtgtactaag caaatcacag ctttaggctc accgctgttc gcttcccatt
     2641 gttgtccgaa aattcgcaat ccgtcgtttg acgtccattc cagtttagta tttttcatgc
     2701 aaagtagttt atatgacaaa attaacttaa atttagtcaa gtaattgtca acgacaaaat
     2761 aatcagatga aaaaatcgag catgctgtta ttaaaattat ccggaatatt tattctactt
     2821 gtaatcatat acttaatctt ggttattatt tatgcccaaa caaataatta tgatccggaa
     2881 cccaaatcaa aggctgaaga tataaacatc tacacgaatc cgatacaaca agatactttc
     2941 gttttttaca cctggaatat cggctattgt ggtcttggtg cagaatccag ttttttcttc
     3001 gattcgccta atcccatcaa gcaatttgtt tatccttttg aggtacatgt ccgcccttcc
     3061 agccagactg tacaaaaaaa tatcatgggc gtactgtcaa ctgttagaga ctgggcaagt
     3121 gatgccgatt ttatcgcctt acaggaagta gatatagact ccagacgcag ttatggcaat
     3181 aatgagttgc gtgctgtttc tcaagtattg actaattata atttctcatt cggcaaaaat
     3241 tatgatgtca attttgtacc gtttccattg ttaaaaccta tgggcggtgt actgggagga
     3301 attgctacat tttcgcgttt ttcgtttcag caaactccga ctcgttattc tttccccgga
     3361 agttttcctt ggccaaaaaa gttatttttt ctggatcgat gttttgttga acatcgtttt
     3421 aatgtaaaaa acgggaaaca actggtgttg cttaatacac acaattctgc atttgacggc
     3481 ggtgttttga aggtgcaaga aatggcttat ttaaaacagt atttgctcaa agagtatgaa
     3541 aaagggaatt atgttgtcgt tggtggtgac tggaatcaaa ttcctcccgg ttatcagcct
     3601 aaaagcaaag aaagtggcta tgaagaactg cccatcgcta tagatttttt accttcagac
     3661 tggaaatttg catttgacaa aaacacaccg accaaccgca aagtggatga gccgtatgat
     3721 gctgccagca cctacacaac cgtgattgat tattttctgc tatcgccaaa tattgagctg
     3781 actcatgtca atggaattgc tgatggattt gcctattccg accaccagcc ggtgcgtatg
     3841 agtttcaggc tgaaatgatt ttattggata tttatgaagg ttattggcga tgtttacttc
     3901 gctttgtttt aggttgtatt ttttggtatt cctattcctg tgtttttata ttcttaccct
     3961 tttgtcttga tacaaaagga gagcaaaaaa tcaagaacaa taaaaggcat ttttgcctgt
     4021 gccagtggct acagctctaa aaatctgctt gtgaaaacta tcttgtcgct tgcaatttgt
     4081 gtttaatatt gtttggtttt agcgcattta aacatattgc ttttcatatt attttgcacg
     4141 atagttttaa gtcgccacga ttttttatcg cctcatgctg tttattgttc aggctaaact
     4201 tgagcgtgtt gccttttgta ataattaata tttttttata ctatgacatg tataatatta
     4261 ctgtttattg tacagaattc gtaggggttg cagggtagaa aaacggttgt cgtcccaatt
     4321 ttttcgggac gaacacttta ttgtgtaaag ttcgtagagg ttgcaaagta gaaaaacgga
     4381 tgtcgcatat ccgaacgctt agtttcgtat tgttctcgga tgttgctggg catcgtctgg
     4441 agtgttttac aatttaaaac tttttattaa ttttttatct aacttcattg gatagcattt
     4501 attagttgta cctgataatt catataaatt caaaacagag ctatcatttt ctaaaatcca
     4561 atattttaat aaccctttag ttgaaatgaa attaatttca cctaattcaa aattacactc
     4621 acaaccaatg ttattttcca tagtaatatc ttgcagagat ttatatcctt tattttttag
     4681 cgcagtatct attaaagcaa tattttgctt tgcataagta gaaaaatcta tgctatcaga
     4741 agtataatac tttgtaaaaa actctttacg attattttca tgaatatttt ctaatgtatc
     4801 attactaatt aaaaaaaaac caaatttact catctcttgc aagtttatcg atttgatata
     4861 ataacgattt gtaccttttt tatacaaata atattctcca tactcaggat cagaattgga
     4921 tataccccaa aatcgagatg cctgtttatt tgttgtgata ataaaaataa aagattgcgt
     4981 actattaata cattcacaag gcaacttact ttgtaaacat ttagaataat taaggtttac
     5041 ccaatagtta tatttatctt gcgaaacaca acttgttgta aatgctaatt gaaataatag
     5101 ataaaataaa tttatattaa atgatatata tttcatatta gtttggattt tttcgaactt
     5161 gacttgcttc atttgaactc acccctcgtc ctcggcaggt gttatcacct gctgactagc
     5221 attctgtaaa atacaacact ttatcatatt ttctcctcct ccattagtat ttgtttaacc
     5281 caatattcgt ttgtagtgtt ttataatgct taaactatac ttcaactttg ggttactgaa
     5341 tatataaatc taagttacaa taaaatgaat aactaagaaa aattaaaatt tatttttgtt
     5401 tcggttggtg agaacaccaa ccgagtggaa aaacaatgat ttgaaagaaa taatatttgt
     5461 atcggttgga gtcctcttca accgactcac caatcgatag taaaaaagtt aacttttttt
     5521 attttcaata aatatcgtaa tattgcaata aatagataaa tgggaactac caaaacacat
     5581 caatatacca aaaaacagaa tgaactggct atcatgctga aagctattgc ccaccctgcc
     5641 cgtattgcta ttatcgagtt gttgttaaag aaaaacactt gcatttgcaa tgatatcgta
     5701 gatgaaatac aactagcaca acctacagta tcgcaacatc tgaaagagct gaaacaagcc
     5761 ggctggataa acggagaaat tgagggcact gctatctgct attgtgtcaa tccgaaaatt
     5821 ccgcaaagct tactccatta ttttgatacg atgtatcaaa aatcattatt gaatacggat
     5881 aaatgctgta aataaaaaaa ctatgcaaac taaactcaaa tttttagatc gttttcttac
     5941 cctctggatt ttcctcgcca tgtttgctgg aattttactg ggttatatct ggcctgactt
     6001 ttcaaaaaac atagaacgtc ttagtatcgg tactactaat ttaccgattg ccgttggctt
     6061 aattttaatg atgtatccgc cactggcaaa agtcaattac agtttattgc cccaggcgtt
     6121 taaagaaaca aaaatacttt cgctatcctt ggttttaaac tggatagtcg gcccgatact
     6181 gatgttttta ctcgctatca tttttttaag aaatgagcct ggctatatga gtggattaat
     6241 actgatagga attgcacgat gtattgccat ggtaattgtc tggaatgact tggcaggtgg
     6301 caaccgtgaa tatgccgcat tgctggtagc cttaaacagt attttccaga tgctcactta
     6361 cggattttat gcctggttgt ttatcaattt tttaccgcat tatttcggaa tagcaaattt
     6421 caatattcac gtacccatgt ctgaagtggc taaaagcgtt gccatttatt taggtatacc
     6481 gttttttgca ggcatcagct cgagatatct gctgataaaa aacaaaggtg aagaatggta
     6541 taaccgaaaa tttatcccta aaatatcacc cgttacactt tatgctttgc tgtttactat
     6601 agtaattatg tttagtctta aaggtgataa gattatacaa ttaccctttg aggttataaa
     6661 aattgccttg ccattggtga cttatttcac catcatgttt tttatcagtt ttatgctggg
     6721 aaaattccta catatcccgt atgataaaaa tgcctcagtt tcatttacgg ctaccggcaa
     6781 taattttgag ctcgctattg ccgtctcaat tgccttgttt ggcatacatt ccgcacaggc
     6841 atttaccgga gtcatcggtc ctttggttga agttccggtg ctcattttgt tggttagagc
     6901 gtccaaatac cttcagacaa aattttatac caaatgaaaa tactcatttt atgtaccggc
     6961 aacagttgca gaagtcagat ggcgcatggc atattgcaat cgctgagtga aaaaattacc
     7021 gtttgctccg ccggaacaca tcctgcaata gcggttcatc caaatgctat tttagtcatg
     7081 catgaacttg ggttagatat tagctaccat cagcctgtag ctgttgaagc gtatttaaat
     7141 gaaacttggg attacgtgat aactgtatgt ggtggtgcca atgaaaattg tcctgcattt
     7201 attggcaatg taaaaaacag actccatatc gggtttgaag atcccgcgga tgcaactggc
     7261 agtaaagaag aagtgctgga tactttccgg aaaatacgaa acgagatact tgtcgatttt
     7321 tttgcattct ataacacgat ggtgaaaaaa cacacttgat ggttgctatg acaattaatt
     7381 tatatctttg tcgtacaaga cattatgaat attcaatcag aaatacagac acttaggaaa
     7441 gtagtaatac acgaacccga caatggcata gataatattt ctccagagtt ggcagaaaaa
     7501 ttactttatg atgatattgt ttttttacca aggatgatag aagaacatta tcaatttaca
     7561 caaacattaa gacaacttat tgggacagaa aacgtattgg aattcggaca attattacaa
     7621 gatgttttaa gaaatgatgc gatacgtgaa gaagtcatag aatatttatt cgaaaaagaa
     7681 gacttgagtt tgggtaagta tgagaaatta aaacaagttc cggtggagct gcttgcttcc
     7741 gtactaatca caggaatgga tccggaaacg aaagatactc tattgccacc attgcccaat
     7801 cttgtattta cacgagatat aggatgtgtc atcaatgatt atgtagtatt atgtaaagca
     7861 aataaagatg cccgcttgcg tgaaaatttt ctcactaaat atattttgaa atatcatcct
     7921 gagtttgctt gttttaaaaa caaaattgtt gatttcgttc tggacgaaaa tctcggaaaa
     7981 gatgcaggca tttcgattga aggcggtgat atcatgttgg ttcatccacg ccatttgttt
     8041 atcggtgaga gtgaacgcac ttctttggat gccatttttc aattgaaaca atttttattt
     8101 gaaaatgatg tggtggatta tgtgtctgtc gttgaaattc cagacgaaag atattgcatg
     8161 catctggata ccgtgtttac tttgctcgac aaagatactt gtgtaggttt tagcccgctg
     8221 atgtttgaaa aaaatgacaa agtggatatc gttacctatt cgaaagacaa taaacgggca
     8281 gccttgcatc catcgctaaa agaactgatt ttagaaattt atcccggcat acgatgcata
     8341 ccgtgcggca atggattatc tccatttgac agacgagaac aatggacaga cggttgtaat
     8401 tttgttacgg taaaaccggg cgtggcatat agctatgaac gaaatatacg caccttaaat
     8461 gctttgaaag acaatggctt ttccgtgatt tcatctaagc atattttggc agaagaatac
     8521 acactggcag agctggataa aacaattatc acactgtcat ctgccgaact ctccagagcc
     8581 agaggtggcc cacactgcat gacttttcct atatccagaa attaagattt agtcgttttc
     8641 tttgtgtttc gcaacctact taatgtttct ggggtaatgc ctaagaaatt agaaatatcc
     8701 tgtaagttaa aacgctgata aacgtcagga tactggttta agaaaacctc gtatcgttgt
     8761 ttggcatcca aagccagaag tacacgtata cgggcaatca acaaatcgat agtttcatct
     8821 atcattagct gaaagaaatt catgaaatca ggcttatctg ctaccagtct gaaataattt
     8881 tgataagaca atttcaatcc aaaaacattt tctgaagtag cgatataata attgctaggc
     8941 aattgaaaat gaagactttt aacagcagcg caaaaattag tttcggttat aaataagata
     9001 atattttttc ccaaatcgtc ttcaacatat tttatcagcg agccttttac gataaagtac
     9061 aagtccgtac aaacctcgcc tttagacaaa ataatttcat ttttggggta atgaaaatac
     9121 tcgaaataat catagagatt acttacatca ttcgtcgtaa taaaatcaaa gttggctaag
     9181 aactgttgca aatattcaaa gcattcttta gcactgggta aattaggtct ttcaaattga
     9241 actttgggtt caaaattagg atagtataga ttcgacattt agtaaaagtt cgacatttaa
     9301 aagtaagtaa taaagtttag cttgtgcaag ttactactta ataatgaaga ttttacgaca
     9361 gttttctttg aaatattttg acaaatagca agaaaattac atgctatgta tttggaataa
     9421 aaaggttttt tgttaaggtt atgaaaaaat gcctttacgt tgttttaagc cttcagcaat
     9481 taagtctttt attttgtctt tcaccatatc taccttttct gccacaagat attctcggga
     9541 tgtatctccg ctaaatagaa taggttcacc aaaattcatt actacttttg ttgggaaaac
     9601 aacaggcaac agcagaggtg cagccggaaa cttgattatt ttctccaacc aatctacatt
     9661 gccaaagtta ataatactct cctcacagcc tacgataccg actgggataa taggagtatt
     9721 ggtttgcaga gccatataca taaatcccaa accgaatctt tgcagttggt atttttttga
     9781 atacggctta gaaatccctt ttgccccttc cggaaagaca ataaccgctt cctcattttc
     9841 aagcatgcgg atacaatttt ccggatcgcc caaagtacct ccccattgat taaaatacat
     9901 gctgataaat ggcacttgcg gaacaaatcg ttcgtacata cccttgggcg ctctaggggc
     9961 atatttgttt gtaaccaatg cataacccaa taaggtggca tcaataggca attgcccgct
    10021 gtgattggca attatcagca ctcgtccatg tgcaggaata ttttccattc catgggtttc
    10081 taccctgaag tatttatcaa acagaaattt tccggcgctt acaaatggtt taattccatt
    10141 gatattaaaa ccccaagggt cgtagccaaa gctaccaact ttatttggga atttctcgag
    10201 aaactcatgt tttgttgatt tgctattttt tgccatgcat aaaacttact caaacatact
    10261 taaaaatgcc aaggatatca atttacatga taattttttt atggcatatt taacacttat
    10321 tacctaaaat catctgccac aatatcataa gtttcattaa atttgaagta gtgaaatctt
    10381 tttctaaaat agggatatta gtttctttat tcgttttttt aacggctgca cttggtgtac
    10441 ctgtgataga acatgtttgt tacagttcga ataccacgac gattgctctg ttttctgata
    10501 cgtgcgagaa agacattcaa aaaaacagct gttgtaaaga aaaacaaaaa agtacgtcta
    10561 aaaaatgttg tgaaaataaa tacagctatc atcgtttagt ttatcaagga caacaagaaa
    10621 ataatttcca gttaaagttt tcaaaaaaaa taactgccca acctgacttt ttttgttttt
    10681 atcaggcaat atcaaaatca caaatccttt tttcatttcg tgttctaccc ttaccggata
    10741 aaataagggc acaaacaata agcggacttc tttgcactaa cccagagctt ctgcaaatat
    10801 ttcgatgtta gacaatagca tttactaatc tattgtttaa ctgaaatctt ttacacatga
    10861 aaaaatacta cacgtatttt tggtgtttac taagtagcag cttgtacata ccattatacg
    10921 cacaacaatc aataattaaa atcagagtgt ttgatgcagc aacccagcaa gcactagaaa
    10981 gcgctaatgt ttattggaga gatacaacaa tcggcacgag tacagatact aacggattag
    11041 cactcttgcc tcggattcca aatcaaacat ttatgttagt agattattta ggatataaaa
    11101 cagacactat atccattaat caaaaaatag gatattacac tgcttattta cagccttctg
    11161 tcttactgaa taccgttgaa atcaatgcga aaaaacagag taattttata tcctcattgg
    11221 aaacaaaaaa aacggaaaaa ataacattag acgaacttaa aaaagcagca tgctgtaatt
    11281 tatccgaaag tttccagaca aatgcttcta tagatgtcaa ctatcagaat gccgttaccg
    11341 gagcaaaaga aataaaactt ctcggcttga gtggtcttta tgtacaaaac ctgattgaag
    11401 gattacctta catgcgccgc tatagttatg catttggtat agaccaactg ccttcgccct
    11461 ggataaatgc aatatctatt tcaaaaggaa ttcccagtgt aaaaaacgga tatgagggta
    11521 ctacaggctc tatgtcagtg cagcttaaaa atggctttaa tgataaaaac aaactttatt
    11581 ttgatctgtt tggcaatagc gattctcgca tagaaactaa cctgatcatc catcataaaa
    11641 tcaatacgca tctatctaca tcattgatgt ttaatggtgc ttttcgaccg atgaatatgg
    11701 acagaaatga ggatggcttt ttagacaatc ccaaggtaaa acagtataat ttcttaaaca
    11761 gctggaatat ccatcagcct gataagcgcg tggaaggaca atattattta aaagccatta
    11821 gtgaaaacag acaatccggg caaacggatg cccatgttca acatcagacc ggcactttac
    11881 cactttatgc tgtttccata cagacaaaac gctttgaggc atttgccaaa acaggattct
    11941 tactgaaaaa acatgatgcc agtatcggca cacagttttc gggtgtttac catcagcaag
    12001 aatcacagta tggatcgatc aattacaatg tacgtcaggc ttcatttacc ggaaacttgc
    12061 tctatcaaac cgacatcaaa aatgaagacc atatgatagt aacggggcta agttttacat
    12121 atgataactt gcggcaacaa ctggacagta cacgtctatt tacctcactg caactgcccg
    12181 gtatttttgc tgaatacacc tataaacacg gtgaacgcat ttcgtttatt gctgcaatgc
    12241 gcttggatta ttctaaacaa acttctttgc aatatgctcc gcgtgtgcat gcacgttttg
    12301 atttggataa aaagcaaaat actactttac ggctttctgc cggaagaggg tatcgggtag
    12361 cagatatttt aaacgaaaac caatttctat tggcaagttc aagaacattt gtttaccagg
    12421 gcaatccgat tacagaagca gcatggaatt atggtttttc acttcatcaa aaatataagc
    12481 tgaaaaaacg ggaaggaagt tttactgtcg acttttttcg aactgatttt accgcacaaa
    12541 ataccataga tattgaaaat gagaatggta ttactacact gtatcctaaa caaggcaaat
    12601 cattttcgaa tagtttgatg gctgaatgga atatggaaat tgcaaaagga tgggatatca
    12661 aactagccta taaatgggaa gatgtgcgga tgacttatca tcatttactg atgcaaaaag
    12721 cgttgcattt tattcaccgc ggcttatttt ctacaagtta taaaataccg aataataaag
    12781 cgcagtttga tttgataatg cctatttatg gtaagtcaag aatgccgaat agttttgcag
    12841 agagcactat caacaaattt tccaatccat atatcttact aaatttccaa attttgacta
    12901 aatttaaaat cggggaattc tttgccggag ttgaaaatat ggccaacttc aaacaaaagc
    12961 acccgatact cggaagcccg ggcacctctt attttgatac ctatcaggtt tatgcgcctg
    13021 tttcaggcgt taccggttat ggtggattta gaatatttat aaactgattt ttaacttaaa
    13081 aaataaaaaa aatgaaagca attaaattat tatccgtttt agtaatcatg ttgattacag
    13141 ccagcttggt atttacatct tgtcatggaa aacattgtaa agaagaaggc aaatgcaaaa
    13201 aagagaaatg cgagaaagaa ggcatgtcag acagtactaa gtcatcatgc tgcaaaaaag
    13261 acagtacaat agctcaataa cattttaaat tcaaagtcat gaaaaacata ctattaatgc
    13321 tacctttctt atttgctgta atttctacac aagcaaaaga aaaagcagaa caattacaat
    13381 tcaaagtaaa tggcaattgc gatatgtgca aagcccgcat agaaaaagcc gcaaaagtaa
    13441 atggagtaaa aagtgcagaa tggaatgaac aaacgcactt tatcattgta aaatttatgc
    13501 catccaaaac aaaccaaact gccattcagc aacatatcgc tgctgccgga tacgatacag
    13561 aagatgtcat agcgagtgac gatgcatatc aggctttgcc gcaatgttgt caatacaccc
    13621 gatagccata tacattaaac tagtcattgt accagattac gagggcggcg cttcgcgccg
    13681 ccctcgctat ttaaactcta tggaattttc attttttatc ttgtttaaga ttatttt
//
LOCUS       DAOQII010000039        13583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2201_length_13583_cov_5.439311, whole genome shotgun sequence.
ACCESSION   DAOQII010000039 DAOQII010000000
VERSION     DAOQII010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_13583_cov_5.439311"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..598)
                     /gene="ruvC"
                     /locus_tag="PLF48_03195"
     CDS             complement(32..598)
                     /gene="ruvC"
                     /locus_tag="PLF48_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNF48361.1"
                     /translation="MALSKKIILGIDPGTQITGYGIIISDGVHLELAAIGSIYTEKYD
                     DHYDRLAKIFDEVSEIIKTYKPHVLAIEAPFYAKNVQSMLKLGRAQGMAIAAALSKKI
                     PIQEYSPKKIKQSITGNGNAAKEQVAAMLGHILKTDALPKQLDATDGLAVAVCHHFSL
                     IGIPKDGKRYSGWEAFLRDNPARVKEKR"
     gene            627..1595
                     /locus_tag="PLF48_03200"
     CDS             627..1595
                     /locus_tag="PLF48_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNF48362.1"
                     /translation="MQRVLQNKLLNRLLKALIFVLLFWALYVQVFKNEKLNFVIHHFQ
                     RQFSGNYLLLILVLLLMLLNWGIETAKWKYLINKWHELSWLSAIEGILFGITFSMFTP
                     NRVGEFGGRVWALKTDRITAIVSTLIGSYAQLVVNICLGAIAFTIYILTLPHEPFYLK
                     LILCFLLILVVILTHLTLYNIDFVSTKFSRIKIFSKISNYTEVINKYEARDIFYLEVL
                     SVFRFIIYSIQFILLLHFFGITIPFAKGLIITATIFFLQTINPFSVDLLDIGFRGNVA
                     VYFLSSFTNNELGILAATLCLWLINLVLPAIIGGFSVLQFKFLREE"
     gene            1637..3148
                     /locus_tag="PLF48_03205"
     CDS             1637..3148
                     /locus_tag="PLF48_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNF48363.1"
                     /translation="MSNETGLLDDDILKADSTTYLADAVKGHPKQLILLFFTEMWERF
                     SFYGMRALLVLFMVHQLKYADAKANLIYGTYTALVYLMPLFGGIAADKILGYRRAILL
                     GGFLMACGHLILAIPTEWSFFAGMAFLISGNGFFKPNISSIVGKMYRPGDPRRDGAFS
                     IFYMGVNLGAFFGGLLCGYVGENINWHYGFGLAGIFMVIGLITFSIGQKSLGDLGLTP
                     VKEDGTRVEMPKQALVVLASFLIIPIFIWLFNNYTIMGKIMFPICIIATIVMLIIAFQ
                     QDVVWRNRMITAIILVAFSVLFWAFYEQGGGSLNLYTERNVNTLGLGAAALNNSINPF
                     YIILLSFPFAWMWYNLAKKDKEPSDPMKFSLAFLQLGLGFFIFVIGAKLAFNGTVSFF
                     WYALGYLLLTTGELCLSPIGLSMITKLSPPKFTGMMMGFWFLASALGQHLAGWIGTLM
                     AIPHEGDTASIDKVASLAIYSGVFMKIFYVALGGCLVLLLLVPTLKRWVGGAK"
     gene            3152..3640
                     /locus_tag="PLF48_03210"
     CDS             3152..3640
                     /locus_tag="PLF48_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48364.1"
                     /translation="MKGKIFTIAGIVLIFGYFIWDSMNDPIKNPGAVVLSGNDTLGRD
                     WGAAADAKEKATNEKNQANATESGPSATTASKDLLNGQNPYQTERYTPNYQTMQSADG
                     RTIITGNEQQEENPEDRYKSRHELEKGTTNSYPEENPYYTPYGNPYGTNPEQQYQPAQ
                     PY"
     gene            3663..5723
                     /gene="scpA"
                     /locus_tag="PLF48_03215"
     CDS             3663..5723
                     /gene="scpA"
                     /locus_tag="PLF48_03215"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNF48365.1"
                     /translation="MKISIPQLSFDELLNQQKSNECTVQDYNTGAAGFAPFLRGPYSS
                     MYVHQPWTIRQYAGFSTAEASNAFYKRNLTQGQKGLSVAFDLATHRGYDSDHERVFGD
                     VGKAGVAIDTVEDMKILFNEIPLDKISVSMTMNGAVLPIMAFYIVAAEEQGIDKSLLS
                     GTIQNDILKEFMVRNTYIYPPEASMKIISDIFAYTSKYMPKFNSISISGYHMQEAGAT
                     PEIELAYTLADGLEYIRTGLEAGLKIDDFAPRLSFFWAIGMEHFKEIAKMRAARVLWT
                     KIVAQFNPASAKSLALRTHCQTSGWSLTEQDPFNNITRTCIEALSAALGGTQSLHTNS
                     FDEAIALPTDFSAKIARNTQLYLQNETGICHTVDPWAGSEYLEQLTQEIAEKAWALIE
                     EVESHGGITKAILAGIPKMRIEEAAAKKQARIDSKQDIIVGVNAFQTDAIAEFEILEV
                     NNDEVRATQIARLQQIKKDRNADEVQNSLQKLKDAAQTSSDNLLACCVEAARARATLG
                     EISAVLEEVFGRHKARIQTISNVYAQAIQMNDEFLKARQLSDQFAEKHGRRPRILVAK
                     IGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPKEVAWQAAENDVHLVGVSSLAAGHK
                     TLIPQLIEELKKIDRADIQVICGGVIPRQDFDFLFTAGVLGIYGPGTVLAKAAIEILD
                     KISR"
     gene            5828..7408
                     /locus_tag="PLF48_03220"
     CDS             5828..7408
                     /locus_tag="PLF48_03220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF48366.1"
                     /translation="MEGFRALGLSEEILIALEKKGFEQPTPIQEQTIPLLLKGKNDIV
                     GQAQTGTGKTAAFGLPIIERFDPTQKSVQALILTPTRELALQVAEEIRAYKGSKKIWI
                     TTVYGGAPIGRQMSELEKGAHIVVGTPGRVIDLIERRKLILENVRYVVLDEADEMLNM
                     GFIDDVELILKSCYPERRMLLFSATMPSRILQLAKRYMGNYDLVQIQKQEITNINIEQ
                     SYFEVPERDKFDALCRILDVEEDFYGIIFCNTKAEVVEISNHLNEKGYTADALHGDIQ
                     QNLREKIVKSFKSNKYGVLVATDVAARGIDIQDLTHVINYGLPQDPESYVHRIGRTGR
                     AGKTGKAILIIDPSERRKLVFIQKINKTHMQKGKLPDAFEMVKIKKRRIKDEVETIIA
                     TQHQEDYLSYAMELLQENEGKDVVAALLKHFVKDELNPKAYKQIKESKVSDDLRKQHV
                     KLFYAKGRKDNITPRIILDTLKKDTGIDSRKINDIRIMDAFSFFVTNGEDAEFILSYY
                     KKDKSRKMPLVERARERR"
     gene            complement(7641..8489)
                     /locus_tag="PLF48_03225"
     CDS             complement(7641..8489)
                     /locus_tag="PLF48_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48367.1"
                     /translation="MKHIFISFYLFAFYSCCTEHTPAPNNILAGKIKRITLIQLDSTI
                     QYKDTLHFNFYYNQSGFCNLINSDESNWQMYIEKLNDSLLKIKAIQGPFTFYNYVILD
                     AGGRVSKINKLDTSTQQQSLIVNFLYNQNGGIDSISTYFPTGDPFAVDVSNYDYVYNG
                     SNYTNTKLHWLSPVSGNQEYSKSMACTYTSFQNNKNIPYQVPYYEPVSENEPVAYMAF
                     GGTNFILYVLGLNDIKALMPNNNLTAKRNTASYNYSFNSNNMVTDMQVVDEINPLVQK
                     IKLEYY"
     gene            complement(8538..9386)
                     /locus_tag="PLF48_03230"
     CDS             complement(8538..9386)
                     /locus_tag="PLF48_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48368.1"
                     /translation="MKNIIIITVILCLYGCCKDDAPSSGLTGKLKTLTIIDNYTNSQR
                     QLTCLYDSMGEFYALTSDSLSFTFTTTSSNYAFLDIDVLATDPIFNSKQKLKFNISPI
                     KQVENIYLVDSILQTETLLSNVFYNNHHTIDSVKYAGNSNFVHDIVNSNYNSDGQNYI
                     QQDISWNIFTILGGDRHYVDTITYLYTNFTNNNYIPQQIPLLETGYALLGSTQNNLHF
                     YLAQLKGYSLLNKNKNLIKTVKSKTLGFITNYEYSFNSKNQVIEMRVSKPDGNGTALM
                     YKMTYY"
     gene            complement(9442..10323)
                     /locus_tag="PLF48_03235"
     CDS             complement(9442..10323)
                     /locus_tag="PLF48_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48369.1"
                     /translation="MKFYLIILSMVVYISCTKECDTTSNQLLGKFKSLTVISVGSTDS
                     AKTRIEFYYSANKIDSLSYILSRFDANGNPIKILYSLKMSYLNNAILLSQHYLNYVLS
                     YRKYKVYLQNNPFNRIYEVDTLTNAEYPYLSCVFNTKLDTIYEPAVYPTALNRYCYDY
                     VLEDGNYTQFKFDYDVATFLGGLYHVADTAFITYTTLPFNKYAPMQNMYSSSTLFNLG
                     APGEVIYDFNYILGFEDYYLYPHNQNLIQSIRTVHDSTTIVYFNYEFNTQNQLSKVKI
                     NFREPTSTFLEYDYEYY"
     gene            complement(10332..11897)
                     /locus_tag="PLF48_03240"
     CDS             complement(10332..11897)
                     /locus_tag="PLF48_03240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48370.1"
                     /translation="MINSKFKIQKILLAAMMLSVFVVFYACKKVEDKIRTDTSKTAST
                     NKKGNSSSDRVGAPIDSLEYYSNLFSPTGAVKDFIKQGGVLPITKYVKDYRDLLYQKN
                     IGNGSFPYVCSNGIVVLGYPGAVCPGGGTGSKLNLNNSSGLDCPTYILDPYGNKIYAF
                     SMYDDKDSRQFYYVYLPDNFNANSPIVVLIHGGGWFSGPNPDNTNGWQLPFSFTPSQT
                     NFVKDLLSDSFVVVVPLYRLSRYGNTEPEITSNPVICQDQLNDIDSAIVNVRRNFPSC
                     LGINANSIQVVGESAGGHLALMWAYTKTTVPSAYIKSVVDMYAPTNMQQYGNYLMNIL
                     NTFVCGSIFWPFNNFFPWYFPIPDLTNPLETYNTTVFDCKAQGYANSRVLQSYKMIKS
                     IVAISPITTPLTSTDLANVSPCFSLVSSRVIPTFVMHGKNDNLVPYANSTSTMDTKLT
                     NTGGLLFNFTSSAGSVPTSYTSSLKHGIKQYDNANHGWATTDNNPITHNFSQTILYNL
                     VHADARKWLNGHK"
     gene            complement(12274..12903)
                     /locus_tag="PLF48_03245"
     CDS             complement(12274..12903)
                     /locus_tag="PLF48_03245"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF000736.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNF48371.1"
                     /translation="MYISRFHYLTQDILGYLHVEQVRLACENGVDWIQLRVKNKQRDE
                     VIQIAKEAIAICKQHKVTLIINDHVEICKAVDADGVHLGKTDMPVKEARMFLGKDKII
                     GGTANTIEDIIRLYDDGVDYAGLGPFRFTRTKQNLSPVLGVEGYEKILTEMRNKYIQT
                     PVIAIGGIRIDDVGMLMKSGIHGIAVSSAVHYVEDKLDVLKGFMSVFLD"
     gene            complement(12905..>13583)
                     /locus_tag="PLF48_03250"
     CDS             complement(12905..>13583)
                     /locus_tag="PLF48_03250"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNF48372.1"
                     /translation="SDIKTFQQTGVYGLAATTCITYQHDTHFYDVHWLSFSKIKKQLL
                     PLFKAYKINWVKIGLIKDSSTLIKLLKFLIEKNENIKIIWDPILRSGSGFNFKQKVTK
                     KELKFILDTVFMITPNWNEVQPLSKENDVIAGAQKLAQHTHVYLKGGHNIETPGTDMI
                     WINKKLDILHPAEITNLQKHGTGCVLSSALTAYMAQGNSVHQSCELAKAFTLKYITSN
                     PTNLGYH"
BASE COUNT         4156 a         2472 c         2623 g         4332 t
ORIGIN      
        1 aaatcggaaa ataagtgcaa caataagact gttatctttt ttctttcacc ctagcaggat
       61 tatctctcaa aaaagcctcc catcccgaat agcgtttgcc gtcttttggg atgcctatca
      121 atgaaaaatg atggcatacc gcaacagcca aaccatctgt ggcgtcaagt tgtttgggca
      181 gtgcatccgt tttcaggata tgccctaaca tggctgccac ctgctcttta gctgcatttc
      241 cgttacctgt tatactttgt tttattttct ttggagaata ttcctgaata ggaatttttt
      301 tgcttaaagc ggccgctatc gccatgcctt gcgccctacc cagctttaac atcgattgca
      361 catttttagc ataaaaagga gcttcaattg ccagaacatg gggtttgtat gtctttatga
      421 tttcagatac ttcgtcaaaa atcttggcta atcggtcata atggtcatca tatttttcgg
      481 tataaatact gccgatggca gccagctcta aatgcactcc atctgaaatg ataatgccgt
      541 atccggttat ctgtgttccc gggtcaatgc ccagtattat ttttttgctc aatgccactt
      601 tgtaaagtta tatctttgcc cgactaatgc aacgtgtact ccaaaataaa ttgctcaaca
      661 gactgttgaa agctctgata ttcgtactcc ttttttgggc tttatacgtg caggtattca
      721 aaaacgagaa acttaatttt gtcattcatc attttcagcg acaattttca ggcaattatc
      781 tgttgctgat acttgtcttg ctgttgatgt tgctgaactg gggtatagaa acggcgaaat
      841 ggaaatattt gataaacaaa tggcacgaac tttcctggct gagtgcaatt gaaggtattt
      901 tgtttggcat tacattttcc atgtttacac caaatcgcgt aggagaattt ggcggcagag
      961 tatgggcgct gaaaacagac cggattacgg ctatcgtatc tacactgatt ggcagctatg
     1021 cccagttggt ggtcaatatt tgtttagggg caattgcatt taccatctat attttaaccc
     1081 ttcctcacga acctttttat ttaaaattaa ttttgtgctt tctgctcatc ttagtggtaa
     1141 tactaacgca tctcactttg tataatatag attttgtgag cactaaattt tcgcgaataa
     1201 aaatattcag caaaatcagc aattatactg aagtgataaa taagtatgaa gcaagagata
     1261 tcttttatct tgaagtatta tccgtattcc ggtttattat ttattccatc cagtttattt
     1321 tattacttca cttctttgga attactatac cctttgcaaa aggcttgatc ataacagcca
     1381 ccatcttttt tttgcaaacc atcaatccat tctccgttga tttattggat atcggtttca
     1441 ggggaaatgt agcggtttac tttttaagtt catttacaaa taatgaattg ggtattttag
     1501 ctgccacact atgcctgtgg ctcattaatc tggttttgcc ggccattata ggcggatttt
     1561 ccgtcttgca atttaaattt ctgagagagg aataaattta tttaaatgtt tatctttagc
     1621 gattaaaatt cttagtatgt caaacgaaac aggcttatta gacgatgata ttttaaaagc
     1681 tgattcaacc acttatctgg ctgatgcagt aaaaggacat ccaaagcaat taatattatt
     1741 gttttttact gaaatgtggg agcgattctc cttttacggc atgcgtgcac ttttggtatt
     1801 atttatggta caccaattga aatatgctga cgcaaaagcc aacttaatat acggcacata
     1861 cactgcgctg gtttatttga tgccattatt tggcggtata gccgccgaca aaattttagg
     1921 ctaccggaga gccatcttgt taggtggatt tttgatggca tgcggacatc ttattttagc
     1981 catacctacg gaatggtcat tctttgcagg aatggcattt ctgatttcag gaaatggatt
     2041 ttttaaaccg aatatctcca gtatagtcgg taaaatgtat cgccccggag atccgcgcag
     2101 agacggagca ttttctatct tctatatggg ggttaatctt ggagcattct ttggtggctt
     2161 attatgtggt tatgtaggcg aaaatattaa ctggcattat ggctttggct tagccggaat
     2221 atttatggtt attggcttaa ttacatttag tatagggcaa aaatcattgg gcgatttagg
     2281 gcttactccg gtaaaagagg atggcaccag agtggaaatg ccaaaacaag cattggttgt
     2341 attagcttcc ttcttaatta ttcccatttt tatctggcta ttcaacaatt acaccatcat
     2401 gggaaaaatc atgttcccaa tctgcattat tgcaacaata gtaatgctga taattgcctt
     2461 tcagcaagat gtagtatgga gaaacagaat gattacagcc atcatactag tggcattctc
     2521 ggttttattc tgggcctttt atgagcaagg tggcggttct cttaatttat atactgaacg
     2581 taatgtaaat acccttggct tgggtgcagc agccttaaac aattccatca atccctttta
     2641 tatcattctt ttgagtttcc cattcgcttg gatgtggtat aatttggcaa aaaaagacaa
     2701 agaaccctca gatcccatga agttctcttt ggctttttta caattaggtc ttggtttctt
     2761 tatatttgta ataggagcaa aattagcttt taatggtaca gtaagctttt tctggtatgc
     2821 gctgggatat ttgttactca ctacaggtga gctttgctta tcacccattg gattatctat
     2881 gatcaccaaa ttatcccctc ctaaatttac cggtatgatg atgggattct ggtttttagc
     2941 ttcagcatta gggcagcatt tagccggctg gattggcaca ttaatggcaa taccacatga
     3001 aggcgataca gcgtcaattg ataaagttgc ctcacttgcc atttacagcg gagtctttat
     3061 gaaaatattt tatgtagcat taggaggatg tttggtatta ttattgttgg tacctacatt
     3121 aaagcgctgg gtaggtggcg caaaatagaa tatgaaagga aagattttta ccatagcggg
     3181 catagttttg attttcggat attttatctg ggattcaatg aatgatccaa ttaaaaaccc
     3241 aggtgctgtg gtattatccg gaaatgatac tttaggacgc gactggggtg ccgccgctga
     3301 cgctaaggaa aaagccacta atgaaaaaaa ccaagctaat gctactgaat ccggtccttc
     3361 tgcgactact gcatctaaag atttattaaa cggacagaat ccataccaga ctgaaagata
     3421 tacacctaat taccaaacga tgcagtctgc agatggacgt acgattataa ccggaaatga
     3481 gcagcaagaa gaaaatcccg aagaccggta taaatccaga catgagttgg aaaaaggtac
     3541 aacaaacagt tatccggaag aaaaccccta ctatacacct tatggcaatc cttatggtac
     3601 taatcctgag caacaatacc aacctgcaca gccgtattag ttgaatttcg tatctttagg
     3661 taatgaaaat cagcattcct caactttcat ttgatgaact tttaaatcag cagaagtcaa
     3721 atgaatgtac tgttcaagat tataataccg gagctgcagg tttcgctcct tttcttcgcg
     3781 gaccgtattc ttccatgtat gtccaccaac cctggactat acgtcagtat gcgggttttt
     3841 caacagccga agcatccaat gcattttata aacgcaactt aacacaaggt caaaaaggct
     3901 tatctgtcgc ttttgatctg gctactcacc gcgggtatga ttccgaccat gaacgtgttt
     3961 ttggtgatgt aggcaaggcc ggggtagcta ttgacacggt ggaagatatg aagattttat
     4021 tcaacgaaat cccattggat aaaatatctg tatctatgac catgaatggg gcggttttgc
     4081 ccatcatggc attttatatc gtagcagcag aagagcaagg catagacaaa tctctgctga
     4141 gcggcacgat acaaaacgat attctgaaag aatttatggt gcgcaatacc tatatttatc
     4201 cgcctgaagc ctccatgaaa atcatctcgg atatttttgc ttataccagc aaatatatgc
     4261 ccaagttcaa ttccatttct atcagcggct accacatgca agaagcagga gcgacaccag
     4321 aaatagaact tgcctatacg ctggctgacg gtttggagta tatacgcacc ggattagaag
     4381 caggcttgaa aattgatgat ttcgcacccc gtttatcttt tttctgggct attggcatgg
     4441 aacattttaa agagatagcc aaaatgcgtg cagccagggt attatggaca aaaattgtcg
     4501 cacagtttaa tccggcttca gcaaaaagtt tagctttgcg cacgcactgc caaaccagcg
     4561 gttggagctt aacggagcaa gacccgttca ataatattac ccgcacttgt atagaagcac
     4621 tttctgcggc attgggcggt acacaatctt tgcataccaa ttcttttgat gaagccattg
     4681 ccttgccgac agatttttct gcaaaaatag cacgcaacac ccagttgtac ctgcaaaatg
     4741 aaacaggcat ttgccatacg gtcgacccgt gggctggcag tgaatatctt gaacaattaa
     4801 cacaggaaat tgcagaaaag gcctgggctt tgattgaaga agtggaaagc cacggcggta
     4861 ttacgaaggc aattctggca ggtattccta aaatgcggat tgaagaagct gcagcaaaaa
     4921 aacaagcccg tattgacagc aagcaagata ttattgttgg tgtaaatgcc ttccagacag
     4981 atgccatagc tgagttcgag attttggaag taaataatga cgaagtgaga gcaacccaga
     5041 ttgcccgact acaacaaatc aaaaaagaca gaaatgcaga tgaagttcaa aacagcttgc
     5101 aaaaattaaa agacgcagct cagacaagta gcgataatct attggcatgt tgtgtggaag
     5161 ccgccagagc cagggctaca cttggtgaga tttctgctgt attggaggaa gtatttggaa
     5221 gacataaagc ccgcatacaa accatatcaa acgtttacgc acaagctata caaatgaatg
     5281 atgaattttt gaaagcccga caactctccg atcagtttgc cgaaaaacac ggtcgacgtc
     5341 cgcgcatttt ggtggcaaaa atagggcaag acgggcacga cagaggggct aaagttatag
     5401 ccaccggttt tgccgatttg ggatttgatg tagacatcgg accattgttt cagacgccga
     5461 aagaagtggc ttggcaggct gccgaaaatg atgtacacct cgtaggtgtg tcttcattgg
     5521 cggcagggca taagacgtta atacctcaac taatagaaga attgaaaaag atagaccggg
     5581 cggatattca ggttatctgt ggcggtgtaa tacctcgtca ggactttgat ttcttattta
     5641 ctgcgggtgt actcgggatt tacggacccg gcaccgtgct ggcaaaagcc gctatagaga
     5701 ttttagataa aataagcaga taaattaaat ttttgataat ttatgacctc gtaggttggc
     5761 ataacgaaac cttaatctgc tttccgtttt aatggtcgta cctttgcgct tcacttaaaa
     5821 taaaataatg gaaggattca gagcattagg tttaagtgag gaaatattaa ttgcattaga
     5881 gaaaaaaggt tttgagcaac ctactcccat tcaagaacaa actattccgt tactcttaaa
     5941 aggtaaaaac gatatagtgg gtcaggctca aaccggaaca ggtaaaactg ccgcatttgg
     6001 cttgcctatc atcgaaagat ttgacccgac acaaaagtcg gttcaggcac ttatcctcac
     6061 acctacccga gaactggcac tgcaagttgc cgaagaaatc agagcctata aaggttctaa
     6121 aaaaatatgg attaccaccg tttacggtgg cgcacccata ggacgtcaaa tgagcgagct
     6181 ggaaaaaggc gcacacatcg tggtgggtac gccgggtcgt gttattgact tgatagaacg
     6241 ccgtaaatta atcctggaaa acgttcgtta tgtcgtgctg gatgaagccg acgagatgct
     6301 gaacatgggc tttattgacg atgtagagct cattttgaaa tcttgctatc ccgaacgcag
     6361 gatgttgttg ttttctgcaa cgatgccatc acgcattttg cagttggcaa aacgctacat
     6421 gggcaattac gatttggttc aaatccagaa acaggaaatt accaatatca acattgagca
     6481 gtcgtatttt gaagtgcctg agcgcgataa attcgatgct ttatgccgga ttttagatgt
     6541 ggaagaagat ttttacggta ttattttctg caatactaaa gcagaagtag tggaaatatc
     6601 gaaccacctg aacgagaaag gctatacggc agatgcgctg cacggcgaca ttcagcaaaa
     6661 cctgagagag aaaatcgtga agtcgtttaa aagcaataaa tacggcgttt tggtggctac
     6721 ggatgtggca gcccgcggta tcgacattca ggatttgaca cacgtaatca actatggctt
     6781 gccacaagac cctgaaagtt atgtacaccg tataggtcgt acgggacgtg ccggaaaaac
     6841 cggaaaagca attttgatta ttgacccgtc tgagcgaaga aagctggtat ttatccagaa
     6901 aatcaataag acacatatgc aaaaaggcaa attgccagat gcctttgaga tggtgaaaat
     6961 caaaaagcgt agaatcaaag acgaggtgga aacgataatt gctacgcaac atcaggaaga
     7021 ttacttgtcg tacgcgatgg agttgttgca agagaatgaa ggtaaagatg tggtggctgc
     7081 attgttaaaa catttcgtga aggatgaact taacccgaaa gcgtacaaac aaattaaaga
     7141 aagcaaggtg agtgatgatt tgcgcaagca gcatgtaaaa ttgttttatg ccaaaggaag
     7201 aaaagacaat attacgccgc gcattatttt agataccttg aaaaaagaca cgggcattga
     7261 cagcaggaaa ataaacgata tccgtatcat ggatgcgttc tctttctttg ttaccaatgg
     7321 cgaagacgcc gaatttattt tatcttatta caagaaagac aaatcgcgca aaatgccgct
     7381 ggtagaaagg gcgagagagc ggagataaac gtattattct ttttaaatac aaagcgaatt
     7441 tgtaaatata tttacaaatt cgcttttttg atttttagta ttttgttttg tcaaaataga
     7501 ttagtaacct gagttcggga taaaaatagt tgatttttga tatagaacca cctccttttt
     7561 attcctcctc cttgttaagt tggaggaaaa atgagaaata aactactttt ttatctttat
     7621 attcttaacc cgaactcagg ttagtaatac tctaacttta ttttctgtac gagcggattt
     7681 atttcatcaa caacctgcat gtcagttacc atattattag aattaaatga gtaattgtaa
     7741 gaagctgtat ttcttttagc agttaaatta ttgttgggca ttaatgcttt aatatcattt
     7801 aatcctaata catataagat aaaatttgtt cctccaaaag ccatataagc aacaggttca
     7861 ttttcagaaa cgggttcgta atagggtact tgatacggga tatttttatt gttttggaat
     7921 gaagtataag tacaagccat tgattttgaa tattcctgat tcccactaac tgggctaagc
     7981 cagtgcaatt ttgtattcgt gtaattagaa ccattataga cataatcgta attcgaaaca
     8041 tctacggcaa acgggtctcc ggtaggaaaa taggtagata tggaatcaat gccaccgttt
     8101 tgattataaa gaaaattgac tatcaatgac tgttgctgag tggatgtatc tagtttgttt
     8161 attttagaaa ctcttccgcc tgcatctaat atgacatagt tataaaaagt aaatggtcct
     8221 tgaattgctt taatctttaa aagactatca tttagcttct caatatacat ttgccagttg
     8281 ctttcatccg aatttatgag attacaaaaa ccggattgat tgtaataaaa gttgaagtga
     8341 agcgtatcct tatactgaat tgtactatct aattgtatta aagtaattct ttttattttt
     8401 cctgctaaaa tattattggg cgcaggtgtg tgttctgtac aacaactata gaatgcgaat
     8461 aaataaaaag aaataaatat atgcttcatg tttttatatt attgcaataa ttatatttag
     8521 ttgattttgt cttttaatta gtaataagtc attttataca tcaaagctgt tccatttcca
     8581 tccggttttg aaacacgcat ttctattact tggttcttag aattaaagga atattcatag
     8641 tttgtaataa aacctaatgt ttttgatttt acagtcttga ttaggttttt atttttatta
     8701 agcagagaat acccttttaa ctgtgctaaa taaaagtgta ggttattttg tgtacttcct
     8761 aataatgcat atcctgtttc taaaagagga atttgctgtg gaatataatt attgtttgtg
     8821 aaattagtgt ataaatatgt gatagtatca acatagtgtc tatctcctcc taaaattgta
     8881 aatatattcc atgagatatc ttgttgaata taattttgtc cgtctgaatt ataattagag
     8941 tttacgatat catgcacaaa gttgctattt cccgcatatt tgacagaatc aattgtatga
     9001 tggttattgt agaatacatt tgataataag gtttccgttt gtagtataga gtcaactaaa
     9061 tatatatttt ctacctgttt tataggagag atattaaatt tcaacttttg cttactatta
     9121 aaaataggat ctgtagctaa gacatcaata tctaaaaatg cgtaatttga acttgttgta
     9181 gtaaaagtaa aacttagact atcgcttgtt aatgcgtaaa attcacccat actgtcataa
     9241 agacaagtta gctgcctttg agaattggta tagttgtcaa taattgttaa agtctttaat
     9301 ttgcctgtca gaccagaaga tggggcatca tctttacagc aaccatataa gcagagtata
     9361 actgttatta ttattatatt cttcatggct tacaatttat aaccgtttaa ccattgtaca
     9421 atatttgctc tgatatactc attaataata ctcataatca tattccaaaa aagtactggt
     9481 cggttctcta aaattaatct tcacttttga taattgattt tgagtattaa actcatagtt
     9541 aaaataaact atagtggtgc tgtcatgcac tgtgcggata ctttggatca aattttgatt
     9601 atgtggatat aaataataat cttcaaagcc caatatataa ttaaaatcat aaataacttc
     9661 gccgggtgca cctaaattaa acaaagtact ggaactgtac atgttttgca taggggcgta
     9721 tttgttaaaa ggcaatgtag tatacgttat aaatgcagta tctgcaacat gatacaaacc
     9781 acctaagaaa gtcgctacat cataatcaaa cttaaattgg gtataattac catcttcaag
     9841 cacgtaatca taacagtacc tgttcaatgc cgtaggataa acagctggct cgtatatcgt
     9901 atctagtttt gtgttaaata cgcaagataa atatggatat tctgcattag ttaaagtatc
     9961 tacttcatag attctattaa aaggattatt ttgcagatac accttatact ttctgtaaga
    10021 taaaacataa tttagatagt gctgtgaaag taatattgcg ttatttaagt aactcatttt
    10081 tagactatat aatattttta tcggattacc attagcatca aatctgctta atatatagct
    10141 taatgaatca atcttattag cactataata gaattctatt cgtgtcttag cactatctgt
    10201 agatcctaca ctaataacag taagcgattt aaatttgcct aatagttgat tggaggttgt
    10261 atcgcattct ttcgtgcatg aaatatatac caccatgctt agaattatta aataaaattt
    10321 catagttgga attatttatg tccgtttaac cattttcttg catctgcatg taccaaatta
    10381 tacaagatgg tttgtgaaaa attgtgcgta atcggattat tatctgtggt agcccaacca
    10441 tgattggcat tgtcgtactg tttaattcca tgttttaggc ttgaagtata gctggttggt
    10501 acgcttccag cagaagaagt aaaattaaat aataagccac cagtattagt aagcttagta
    10561 tccatagtac tggttgagtt ggcgtaggga accaagttat catttttgcc gtgcatcaca
    10621 aaagtaggga taactcggga tgataccaaa gaaaaacaag gactcacatt agctaaatca
    10681 gtagatgtga gtggcgttgt aataggcgag atggcaacga tgctttttat cattttatag
    10741 ctttgcaata ctcttgagtt ggcatatcct tgtgctttac aatcaaagac agtggtatta
    10801 taggtttcta aaggattcgt taaatctgga atcgggaaat accatgggaa aaaattgtta
    10861 aatggccaaa atatagaacc acacacaaaa gtatttagaa tattcatcaa ataatttcca
    10921 tattgttgca tattggttgg tgcatacatg tccactacag attttatata agcactaggt
    10981 actgttgttt tagtataagc ccacatcagt gccaaatgtc cgcccgcact ttcgcccact
    11041 acttgtatgc tgttggcatt aatgcctaaa cagcttggga aatttcttct cacattcact
    11101 atcgctgaat ctatatcatt aagttggtct tgacaaatta cagggttgct ggtaatttca
    11161 ggttctgtat tcccatatct tgatagccta tagagtggta ctactacaac aaaactgtcg
    11221 gacaataagt cttttacaaa gtttgtttga gatggagtaa aagaaaaagg taattgccag
    11281 ccattcgtgt tatccgggtt gggtcctgaa aaccaacctc cgccgtgtat gagcactact
    11341 atcggtgaat tggcattaaa attatccggc aaatacacat aataaaactg tctgctgtct
    11401 ttatcgtcat acatagaaaa ggcgtaaatt ttgttaccat aagggtctaa aatataggtc
    11461 ggacaatcta aaccggaact attattcagg tttaacttag agcctgtacc tccacccgga
    11521 caaacagcac ccgggtagcc caataccact atcccgtttg aacaaacata tggaaatgaa
    11581 ccattcccga tgtttttttg atagagtaaa tccctgtaat ccttaacata tttggtaatc
    11641 ggcaatacac ccccttgttt tataaaatct tttactgcac ctgttggtga aaaaagattg
    11701 gaataatatt cgagcgaatc aattggtgcg ccaactctat ctgaggagct gttgcctttt
    11761 ttatttgtac ttgccgtttt tgatgtatct gttcttattt tatcttctac ttttttacag
    11821 gcataaaata ctacaaacac actaagcatc atggctgcta atagaatttt ttgaattttg
    11881 aattttgaat ttatcataaa aggagttcta attaattgta aatataacac tatattttaa
    11941 atattgcaaa ttaattcttt gtcttttttt gaatatagta tgttaaaatg cacccactgt
    12001 ctatatttat gctcttaaca aataaccgat aaacagtctt atcgttgtta acttgagttc
    12061 gggataaaaa tagttgattt ttgatataga accacctcct tttaattcct cctccttgtt
    12121 aagttggagg aaaacaggag aaatcaacta tttcttccac cttaattttc ttaacacgaa
    12181 ctcaggttgt taattatccc attctcataa cttggatatc agcaggaata ttgataaaga
    12241 gataatgcta aacataagta tctaaaatac tttttaatct aaaaacaccg acataaatcc
    12301 cttcaaaaca tccaatttat cttccacata atgcaccgcc gaagaaacgg ctatgccgtg
    12361 aatgcctgat ttcattagca ttccaacatc gtctatacga ataccgccaa tggcaataac
    12421 aggtgtttgg atgtacttgt ttctcatttc tgttagtatc ttttcatagc cttctacacc
    12481 caaaaccggg cttaaatttt gtttcgtccg cgtaaaacgg aatggcccca agccagcgta
    12541 atccacgcca tcgtcataga gtctgatgat gtcttctatt gtatttgccg tgcctccgat
    12601 gattttatct tttcccaaaa acattcgcgc ttcttttacg ggcatatctg tttttcccag
    12661 gtgcacacca tcggcatcta ccgctttaca aatttccaca tggtcgttaa tgatgagtgt
    12721 aaccttatgt tgcttgcaaa tcgcaatggc ttcttttgca atttgaatca cctcatcacg
    12781 ttgcttgttt ttcacccgaa gctgaatcca gtcaacgcca ttctcgcagg caagtcgcac
    12841 ttgctctaca tgcaaatagc cgagtatatc ttgcgtgagg taatgaaaac gggaaatgta
    12901 catgtcagtg ataacctaaa tttgttggat tgctcgttat gtatttcagg gtaaacgctt
    12961 ttgccagctc gcatgattga tgtacagaat ttccttgtgc catgtatgcc gtcagtgctg
    13021 atgacaacac gcaccccgtg ccatgctttt gtaaatttgt tatttcagca ggatgcagaa
    13081 tgtccagctt tttatttatc caaatcatat cggtgccggg cgtctctata ttatgtccgc
    13141 ccttgaggta aacatgggta tgttgagcta acttttgtgc acctgcaatg acatcatttt
    13201 ccttgctcaa aggctgtact tcattccagt tgggcgtaat cataaacacg gtatcgagta
    13261 tgaatttcag ttcttttttt gttacttttt gtttgaaatt aaaacctgag ccggatctca
    13321 aaatcgggtc ccatataatt ttaatgttct catttttttc gattaaaaat ttcagcaact
    13381 taattagtgt gctactgtct tttatcaaac cgattttcac ccaattaatt ttatatgctt
    13441 taaataaagg cagtagttgt ttttttattt ttgagaaaga gagccagtga acatcataaa
    13501 aatgcgtgtc gtgctgatag gtgatacacg tggtggcagc aagaccatat acacccgttt
    13561 gctgaaatgt tttgatgtca gac
//
LOCUS       DAOQII010000040        13223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2297_length_13223_cov_5.146112, whole genome shotgun sequence.
ACCESSION   DAOQII010000040 DAOQII010000000
VERSION     DAOQII010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2297_length_13223_cov_5.146112"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLF48_03255"
     CDS             <1..372
                     /locus_tag="PLF48_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNF48373.1"
                     /translation="NLVAAGVCDEVLVQVSYAIGVAKPCGIFVDTNGTAKVNMTDGEI
                     AKVVEKVFDMRPYAIEQRLKLRNPIYSETASYGHMGRKSEVVTKVFNKGKDNEKKVQV
                     ELFTWEKLDYVDAVKKAFKLK"
     gene            complement(443..1714)
                     /locus_tag="PLF48_03260"
     CDS             complement(443..1714)
                     /locus_tag="PLF48_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF48374.1"
                     /translation="MKQILSNILPFLFLLNNSTATLIAQNYAIQHGTIHVGNGQVIED
                     GVILFEKNKIINVGKFASEADKKYTRIDATGKQIYPGIILANTDVGLVEAEAIRPTRD
                     DYEVNLINTNIRSLIAFNTDSKVLPTYIFNGITYAQVCPRGGLVAGSSSVMKTNGWNW
                     EDAVELADDGIHLYYPSLINWGGKPNEKYTATYNKIETFLADAKAYADTFSLREKVLT
                     LEPMREVFSGNKNLYIHVNGEKEIITAVLLAEKYGIKPVLVGAKDCEKIISFLKEKGC
                     AIILHKMHILPLRVDDDVNAIYKIPNALAEAGILFCFGNNEYENWNARNIMFNAGSAV
                     AFGLQKEEAIKALTYNPAKILKCENQMGTLEAGKNATFLISKGDILDMKSNQLETVFI
                     DGQEVNLNENWQQQLYEKYLQKYKQENSLKP"
     gene            complement(1736..4702)
                     /locus_tag="PLF48_03265"
     CDS             complement(1736..4702)
                     /locus_tag="PLF48_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF48375.1"
                     /translation="MKRLSVLLFLNAVVCLLFAQETFPVNGTPDKRPEKFLLIHARIV
                     PDYKSVPFDGSMLIENGKIKKMAKGIIEEKGAVIIDAKGNYLYPSFIDLYAQYGLAQN
                     TPKSNNGYPQFLSDKQGAYDWNEAIRAEYNAYENFKTDAKQAQSYLENGFGALLTHNM
                     DGIVRGSGALVSLSTQKKEQEIFIQPRAAGFYSFDKGSSQQDYPNSLMGSIALLRQTY
                     YDAQWYKTQKTEFNITLDAFNKLQQMPMFFNTSDWQDVFRAKKIADEFKLNYIYVGNG
                     DEYQRIAELKNLNIPLVIPVVLPLPFDVEAPYVATDISWQQLAHWEDAPYNLALLQQQ
                     KIDFALTSYGLKNNKDFFKNLNKYIKYGASKEEILKALTYTPAKLIGADSLIGTLAVG
                     KYANFILTDKPVFDDGFSILQNWTQGEQHIINPFTDSLYEKYILSYGNRKDTLTINKS
                     VSKNEAFIMADTVQQKLNVSLAENQISFSFDNIDKNNKGTIRFMGWKSINGFSGNALM
                     PDGKSITWMAKSFSFTAKKDSTFVKTDTAKKQLPAIVFPFNAYGNNKLPEAKKIIFKH
                     ATVWTNETEGILKNTDVVVENGKITYIGNQADEKNAIIIDATGKHLTSGIIDEHSHIC
                     ISGNVNECSQSSTAEVRIGDVVNSNDINMYRQLAGGVVAAQLLHGSCNPIGGQSALIK
                     LRWGNMPEELKIKNTDGFIKFALGENVKQSNWGEKQNQRFPQTRMGVEQVYMDYFTRA
                     SEYLQKKNARKDLGLDALAEILNKKRFITCHSYVQSEINMLMHVADKFKFKVNTFTHI
                     LEGYKVADKMKKHGAGASTFADWWAYKLEVMEAIPWNAAVLQKMGIVTAINSDDAEMG
                     RRLNQEAAKALKYGGISEEEAWKMVTLNPAKLLHLDKNMGSIKVGKDADLVLWSDNPL
                     SIYAKAEQTYIDGKKYFDITEEEKMQDQIRKEKARIIQNMLQAKKDGAETQPAMPYFQ
                     ENYHCDDSGIYHERQK"
     gene            4755..5888
                     /locus_tag="PLF48_03270"
     CDS             4755..5888
                     /locus_tag="PLF48_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNF48376.1"
                     /translation="MYQKTSYTAIGLMSGSSLDGLDICAVTFTIDSNAENSTRQTDNY
                     KQPTILPSNIAYSIIAAKCIAFNDAFKDRIRLATQNNAFDFAQLHTEIGKYFGQLTAD
                     FIQEYKLNKIDFIASHGQTIFHQPLLGFTTQLGCGAQIAAQTGCKVICDFRTSDIAYG
                     GQGAPLVPIVEKYLFPQYKAFLNIGGICNISLHSKDDRIYGYDICAGNTLLNHFAKKK
                     GMQYDENGVLARSGKILPDLVDELNQIPFCLQDAPKSLGSEHVYAEWIARCERYNAGI
                     EDILASCTEHIAMQTGKALQHFNNGNAVLITGGGALHTYLTERIQAHTALQAVVPGMQ
                     TLQYKEALLMAFCGLLRFIEIPNSLASVTGAKKNVTGGAIYLP"
     gene            5994..6863
                     /locus_tag="PLF48_03275"
     CDS             5994..6863
                     /locus_tag="PLF48_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF48377.1"
                     /translation="MKNLYTEKTIEKKRELEIIDYYNQCTVDYKLVWHLNSEMCLHYG
                     YWDTSTSNLREAIRNMNIKLAEFGNISANKKILDAGCGAGGSSIFLAQNYQAHVTGIA
                     LPELQIAACKANAQKFGLEHVLHFDVQNYCCTTLPSQSFDFVWAIESVCHAEYKVRFL
                     QEAFRLLKPGGTLIMADFFSNKNPSSWLQKMEKSWSIPSFACTDYFKSEMEKIGFTDV
                     KIEDKSDAVYPSVKRLYACYFPGMLISKTMEWLGLRTKRQTENTRSCLYQYKAFKSGD
                     WNYSFIRAQKPDA"
     gene            complement(6835..8571)
                     /locus_tag="PLF48_03280"
     CDS             complement(6835..8571)
                     /locus_tag="PLF48_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF48378.1"
                     /translation="MGRRNSYIESIIDRLISQGKDKRETLIFFSKLTAFFTFLYAFVS
                     GFIMHNYWSMVECSLYGIFCLLLPTLLKRNYNYRILANLFVLGGVINVYFNAFQFGGI
                     NSSVMTWTVYIPIVPLLIAKDKDAIRWLFLALTVNIIFWLVNYQRGLSDVEYRISLFN
                     LFVYEGLDLLIFLFSFFFYLSRDKAEKILTQKNLELEQTKIELLESQNSKDIFIANIS
                     HEMRNPISIIKGLLNIVEKNEQDENSKVYLNKCVENANQLTSIINDLLDISNLETGRL
                     HTFKANFDLYKLIYASETYFENRVGQKAIQYTIDIHPDTPKLILCDQTRLTQIINNLL
                     DNAIKYTESGNIQLNCFMQDAKICIEVKDTGIGIPEDKLEHIFENFFRVSYDNAVSGT
                     GLGLSIVKKIVKLIDGEIYVTSTMHKGSAFRVEFPYEYDPTLDEPDTEVKLLEIRKKL
                     NGISLLIADDMLLNHLIIENAIHEQLLNCSMDFAIDGEEVLEKLKTSQYDILLMDINM
                     PKKSGIETTQEIRKIYGKKQHIIALSANAQKKDIDYAKKLGFDEYVTKPLDISDLLLK
                     MDSVMHPVSVLE"
     assembly_gap    8904..9003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9004..11181)
                     /locus_tag="PLF48_03285"
     CDS             complement(<9004..11181)
                     /locus_tag="PLF48_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNF48379.1"
                     /translation="MAINKLINIYIIGGGVAGMTAAHELSKKKIDGKKVFNITLFEKD
                     PKYIGGKARSIEHLENNFQDSNRLNFLPAEHGFRFFPGFYSNIDQVLKEIPADNPGNK
                     KNKSVFDNLVQTSTYMFSFFKNQNRNPILLLNKTEQWDYFGSVLKMIRSTRDAYLDIN
                     HNGKNHFAYALFKMITSSHFRKQEEFERESWFDFINARNTSYGNDYATFFADGLTRSL
                     AAAKSRKTSTRTGSTILPKMLFEVIAPGGAAGRVLNAPTNQAWLLPWKNQLEKEGVKF
                     EMNAVCVDIECTYHQELPTVDVEKSALSKNKSKDFYYIESIRIKQDETYTWKHNADMK
                     AIFLFAIPVEKMAKLLTDSRDADIFHADPSLKFIQHLKGSVDWMCGIVFYLRNKVEIT
                     HGHINLNNSKWAITGIHQEQFWTAAYQPERFGNGDVKGILSLIMSNCDEDSDLCDKRS
                     VFEMTREDIIKEAWEQIKAATFIVERDDNGEICGYRCPTDHDIIYEYTFLDESVTERR
                     SEALQEINDYLELTETNRHSEFDTAYADYKKVRNDGADNPLNLHRGKPKKKVFVCENE
                     SKLLVNYVNMYCNRPSTYTRIPNMFLAGDYVRTEVDLATMEGACESGIRATNAIVQTV
                     KGSDLYNDSGEYDKLQLSKIAKYINPFYKFLKTPLFIIFFSLFLIAGFATVLLIFEYI
                     AKHNIFPHLPSLPILKDAIIAGTPLHKIICKVSLVLRYISAAFV"
     gene            11233..11553
                     /locus_tag="PLF48_03290"
     CDS             11233..11553
                     /locus_tag="PLF48_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48380.1"
                     /translation="MAGEIKNRIIDFLKDFETSRKERYILGIADFSEKLSQLTIALKD
                     EEEGQIRFLLHSLKPLSQLLNLSELNLLLHSISKDHNTDYSEIILLLESKIQEIDTTL
                     NSLH"
     gene            11594..12565
                     /locus_tag="PLF48_03295"
     CDS             11594..12565
                     /locus_tag="PLF48_03295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF48381.1"
                     /translation="MILVTGASGFLGGELVKQLVFQGEQVRIIKRKHSDLSHLQAFQN
                     KLEITEGDILDIPSLETACEGIDKIYHAAAVIGYDASYYDKMYKTNIEGTANVVNIAL
                     YKNVKKIVHISSVAAIGGKPDVEIDEETKWEKNEWTTHYGITKMLAEREIWRGVQEGL
                     DAVIVNPGIIIGVGNDEHKATLRLFRQIATRKLPFYTPGSIGFIDVEDCARACIDLME
                     CKKTAERFILVNETLTYKNYFEKIAQALQVAPPQMKLNRYSGRFFVFLDAMLSTFSSR
                     KRGLTAENLKIAMENFHYKNDKFVNQFSFTYTPMDVTINNIAKHIKQ"
     gene            12567..>13223
                     /locus_tag="PLF48_03300"
     CDS             12567..>13223
                     /locus_tag="PLF48_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNF48382.1"
                     /translation="MDFLRKENKNKILGIWYMVGVGMLLGQILLGGITRLTGSGLSIT
                     EWKAILGFIPPMNEQAWNLAFIKYQQFGQFKLVNTSMTLEQFKHIYFWEWFHRLWGRL
                     IGIAALIPLIIFAIKRLVNKQDIFKIVIAIVLGGIAGIVGWIMVQSGLKQNTVLVNPV
                     NLMSHIVVATIIVAYVLYTGLGYLYPNRDKNDNKNKRIYFLLLILLVFIQIALGALVA
                     G"
BASE COUNT         4032 a         2481 c         2312 g         4298 t
ORIGIN      
        1 aatttagtgg ctgcaggtgt atgtgatgaa gtattagtac aagtttctta tgctatcggg
       61 gtggctaaac cttgcggaat atttgtcgat acgaatggta cagctaaagt aaacatgacg
      121 gacggcgaaa ttgccaaagt ggtagagaaa gtatttgaca tgcgtcctta tgctatcgag
      181 caacgtttga aattgcgcaa tccgatttat tcagaaacag cttcttacgg acatatggga
      241 agaaaatcgg aagtggttac taaagttttc aataaaggta aagacaacga gaaaaaagta
      301 caggtagaat tattcacctg ggaaaaatta gattatgttg atgcggttaa aaaagcattc
      361 aaattgaaat aatttttcat cacataaata aaaaaagagc cggaaatagt ccggctcttt
      421 tttttgtcag caactgatta tcttatggtt ttaagctatt ttcctgttta tacttttgca
      481 ggtatttttc atagagttgc tgttgccagt tttcattcaa gttgacttct tgcccgtcaa
      541 taaaaactgt ttctaattgg ttggatttca tatcgagtat atctcctttt gaaatcagaa
      601 aggtggcatt ttttcctgct tcaagagtgc ccatttgatt ttcacacttc aatatttttg
      661 caggattata agtcagtgcc ttaatagctt cctccttttg caagccaaaa gcaacggcac
      721 tgcctgcatt aaacatgata ttccgggcat tccagttttc gtattcatta tttccaaaac
      781 aaaataaaat accggcttca gccaaagcat ttggaatttt atatatcgca tttacatcgt
      841 catcaacacg aagtgggagt atatgcattt tgtgcaaaat aatcgcacat cctttttctt
      901 tcagaaaaga aataattttc tcacaatctt ttgctccgac caaaaccggc tttatgccat
      961 atttttctgc cagtaaaact gctgtgatta tttctttttc tccgttaaca tgtatataca
     1021 gattcttatt tccggaaaat acttcacgca taggctctaa tgtcagcact ttttcccgga
     1081 gtgagaacgt atcagcataa gccttggcat cagctaagaa agtttctatt ttattgtagg
     1141 tagccgtata cttttcgttg ggttttccgc cccaatttat cagggaagga taatataaat
     1201 gaatgccatc atccgccaat tctacggcat cttcccaatt ccatccattc gtcttcatta
     1261 ctgaagaaga accggccact aaaccaccgc gaggacaaac ctgcgcataa gtaatgccgt
     1321 tgaagatgta agtaggcaat acttttgaat cggtattaaa agctatcaat gaacggatat
     1381 ttgtattaat caggttgact tcataatcat ctcttgttgg tctgatggcc tccgcttcca
     1441 ctaagccaac atctgtattt gccaaaataa tgccgggata aatttgtttt ccggttgcat
     1501 caattcgtgt atattttttg tcagcttctg atgcaaactt tccaacattt ataattttat
     1561 ttttctcaaa aagaatcacg ccatcttcta ttacttgacc attacccaca tgaatagtgc
     1621 cgtgctgtat ggcataattt tgtgcaatta gcgttgctgt actattatta agcaagaata
     1681 aaaatggtag tatattactt agtatttgtt tcatcggtcg aaattatgtg gtaatttatt
     1741 tttgtctttc atgataaatg ccggaatcat cacagtgata gttttcctga aaatatggca
     1801 ttgccggttg tgtttctgct ccatcttttt tagcctgtag catattctgt ataattcttg
     1861 ccttctcttt tctgatttga tcctgcattt tttcttcttc agtgatatca aaatatttct
     1921 tgccgtctat gtaagtttgt tcagctttag cataaatact taaaggattg tcgctccaca
     1981 gaaccaaatc cgcatccttg cccactttga tgctgcccat atttttgtcg agatgtaata
     2041 atttagccgg attcaatgtt accattttcc acgcttcttc ttcacttatt cctccgtatt
     2101 ttagtgcttt ggcagcttcc tgattgagtc ttcgtcccat ttccgcatca tcagaattta
     2161 tcgccgttac gatacccatc ttttgcagca cagccgcatt ccacggaata gcctccatca
     2221 cttcgagttt ataggcccac cagtcggcaa aagtagatgc ccctgcaccg tgttttttca
     2281 ttttatctgc tactttatag ccctccagta tatgcgtaaa cgtgttgact ttaaacttaa
     2341 atttatcagc cacatgcatc agcatattta tttcagattg tacataagaa tgacaagtga
     2401 tgaatcgttt tttgtttaaa atttctgcaa gtgcatccaa gcccaagtct ttacgtgcat
     2461 tctttttttg cagatattca gacgctcttg taaaataatc catatacact tgctctacac
     2521 ccatacgggt ttgcggaaaa cgttgatttt gtttttcacc ccaattgctt tgctttacat
     2581 tttcgcctaa agcaaatttg ataaaaccat ctgtattttt aatttttaac tcttcgggca
     2641 tatttcccca tctcagtttt atgagtgcag attgtccgcc tataggattg caggagccgt
     2701 gcaataactg agcggctacc acacccccgg caagctgacg atacatattg atatcattgc
     2761 tattaacgac atcgccgatg cgcacttctg ccgttgaaga ctgtgaacat tcgtttacat
     2821 tcccggaaat acaaatatga gaatgttcat ctataattcc tgaagtgaga tgttttccgg
     2881 ttgcatcaat gataattgca tttttttcgt ctgcctgatt gccgatatag gttattttcc
     2941 cgttttctac aacaacatcc gtgtttttca gaatgccttc tgtctcattg gtccaaacag
     3001 tagcatgctt gaagattatc ttctttgctt cgggaagttt gttgtttccg tatgcattaa
     3061 atggaaaaac aatggcaggc aattgttttt ttgccgtatc tgtttttaca aacgtactgt
     3121 ctttttttgc tgtaaatgag aatgattttg ccatccaggt aattgacttc ccatcgggca
     3181 tgagtgcgtt tcctgaaaat ccattaatag atttccatcc catgaatctg atcgttcctt
     3241 tattattttt atcgatatta tcgaatgaaa atgagatttg gttttcggct aagctgacat
     3301 ttaatttttg ttgtacagta tctgccatga taaatgcttc attttttgaa actgatttat
     3361 taatcgtcaa ggtgtctttc ctgttgccgt atgacaaaat atatttttca taaagcgagt
     3421 cagtaaatgg attaatgatg tgctgttcac cttgtgtcca gttttgcaaa atggagaaac
     3481 catcgtcaaa aacaggttta tccgtcaaga taaaattggc atattttcca actgctaaag
     3541 ttcctatcaa gctgtctgcg ccaattaatt ttgccggagt ataagttagt gctttcaata
     3601 tttcttcttt agacgctccg tactttatgt atttattcag atttttgaaa aagtctttgt
     3661 tatttttcag tccgtaagaa gtcagcgcaa aatcaatttt ttgctgttgt aaaagagcaa
     3721 gattataagg ggcatcttcc caatgtgcca gttgttgcca acttatatct gtagcgacgt
     3781 aaggagcttc tacatcaaaa ggaagtggga gtacgacagg tattacgaga ggaatattga
     3841 ggttcttaag ctcggctatt cgttgatatt catcaccatt tccaacatat atgtagttaa
     3901 gcttaaattc atctgctatt ttttttgcac gaaaaacatc ctgccagtcg gaggtattaa
     3961 agaacattgg catttgctgc aacttattaa atgcatcaag cgtaatatta aattcagttt
     4021 tttgtgtctt ataccattgc gcatcgtagt aggtttgccg aagtaaagca atactgccca
     4081 tgagtgaatt gggataatcc tgctgagaac ttcctttatc aaaagaataa aatccggctg
     4141 cacgcggttg gataaaaatt tcttgttctt tcttttgggt agacaagctc accaaggctc
     4201 cactacctct tactatacca tccatattat gcgtcagcaa agcaccaaaa ccattttcca
     4261 aataagactg cgcttgtttt gcgtctgtct taaagttttc gtatgcatta tattcagccc
     4321 gtattgcctc attccaatca tatgctccct gtttgtccga cagaaactga ggatagccgt
     4381 tatttgattt tggtgtattt tgtgctagac cgtattgtgc atatagatca ataaaagacg
     4441 ggtaaagata attgcctttc gcatctatga tgacggctcc tttttcctca ataattcctt
     4501 ttgccatttt ttttatcttg ccattttcga tgagcatact gccatcaaag ggcacacttt
     4561 tataatcggg tactatgcgg gcatgaataa gcaagaattt ttcaggacgc ttatcaggag
     4621 tgccattcac aggaaatgtt tcctgggcaa acagtaaaca gacaactgca ttcaaaaaga
     4681 gcaaaacgga caatctcttc atattgtgta ttaaagttgt aaatttacat tttgtgattg
     4741 gtttatagat tgaaatgtat caaaaaacat cgtatactgc cattgggctc atgagcggta
     4801 gctcacttga tggattggac atttgtgccg taacatttac tattgattca aatgcagaaa
     4861 attcaactcg acagactgac aattacaaac agcccactat tcttccttca aacatagcat
     4921 acagcatcat tgctgctaaa tgcatagcgt ttaatgatgc atttaaagat cggatcagac
     4981 tggccactca aaacaatgct tttgattttg cacaactgca cactgaaata gggaaatatt
     5041 tcggacaact tactgctgat tttattcagg aatataagtt aaacaaaatc gatttcattg
     5101 cctctcacgg acaaactatc tttcaccaac ccttattggg atttactact cagcttggat
     5161 gcggagcaca aattgcagca caaacgggtt gcaaagttat ttgtgatttc agaacatcag
     5221 atattgccta tggcggtcaa ggtgcacctc tggtacctat tgttgaaaaa tatttattcc
     5281 ctcagtacaa ggcatttctg aatatcggtg gcatttgtaa tatttcttta catagcaagg
     5341 atgatagaat ttacggttat gatatttgtg caggaaatac tttattaaac cattttgcca
     5401 aaaaaaaagg tatgcagtat gatgaaaatg gagttctggc ccgttccgga aagatacttc
     5461 cggatttggt tgatgaatta aatcaaattc ctttttgcct gcaggatgca cccaaatcat
     5521 taggcagtga acatgtatat gctgaatgga ttgcccgatg tgaaagatac aatgccggta
     5581 tagaagatat tttagccagc tgcacagaac atattgccat gcaaacaggc aaagcacttc
     5641 aacattttaa caacggaaat gctgtactca tcaccggtgg cggtgcttta catacatatc
     5701 tgacagaacg catacaggca catactgctt tacaggctgt agtacccggc atgcaaaccc
     5761 tacaatacaa agaagcctta ttaatggcat tttgcgggtt attgcgtttt atagaaatcc
     5821 caaacagctt agcatcagta acgggtgcaa aaaagaatgt tacaggcggg gcaatttatt
     5881 taccataaaa aagcaggaat acctgttaaa ataatcagca taattttttt atttttattt
     5941 tttttaatga aataatctat tatctttatt ggaaataacc ttttaccata aagatgaaaa
     6001 acctttatac ggaaaaaacc attgagaaga aacgtgagct tgaaatcata gactattata
     6061 atcagtgtac agttgactat aaattagtct ggcatctcaa ctctgaaatg tgcctgcatt
     6121 atggctactg ggatacttct acgtcgaacc tccgtgaggc aatcagaaat atgaatatta
     6181 agctggcaga atttggaaat atttctgcaa ataaaaagat actggatgca ggatgtggtg
     6241 caggcgggag cagtattttt ctggctcaaa attatcaggc ccatgtaacg ggtattgcat
     6301 taccagaatt acaaatagca gcgtgcaaag ccaatgctca aaagtttggt ctggagcacg
     6361 tcttgcactt tgatgttcag aattattgct gtacaacatt accctcgcaa tcatttgatt
     6421 ttgtttgggc aatagaaagc gtttgccatg cagaatacaa agtgcgcttt ttacaagaag
     6481 catttcgctt gctgaagccg ggtggcacgc taatcatggc agattttttc agcaataaaa
     6541 atccatcttc atggttgcaa aaaatggaga aatcttggtc cattccttcc tttgcgtgta
     6601 ctgattattt taaatcagaa atggaaaaaa ttggctttac tgatgttaag attgaagata
     6661 aaagcgatgc tgtttatccg tcagtaaaac gattatatgc ctgttatttt ccggggatgt
     6721 tgatcagtaa gactatggaa tggctgggat tgcgcacaaa aagacagact gaaaacaccc
     6781 gttcttgctt ataccaatat aaagctttta aaagtggaga ttggaattat tcatttattc
     6841 gagcacagaa accggatgca taacactatc cattttcaac aataaatcgg agatatcaag
     6901 aggtttggta acatattcat caaaaccaag tttctttgca taatcaatat ccttcttttg
     6961 tgcatttgct gataaggcaa taatatgttg ctttttgcca tatatcttcc gaatctcctg
     7021 cgtagtttca atgccgcttt ttttaggcat attaatatcc atcaataaaa tatcatattg
     7081 actggtcttc aatttctcca atacttcttc gccgtcaata gcaaagtcca tgctacaatt
     7141 caacaactgc tcgtgaattg cattttcaat aatcaaatga ttcaacagca tatcatccgc
     7201 aattaacaaa cttataccat tcaatttctt tctaatctca agaagtttta cttctgtatc
     7261 cggttcatca agtgttgggt catattcgta tggaaattcc acacgaaaag cagagccttt
     7321 atgcatagta cttgtaacat aaatttcacc atctatcagt ttcacgattt ttttcacgat
     7381 agacaaaccc agccctgttc ccgaaacagc attatcataa ctcacacgaa agaagttttc
     7441 aaaaatatgc tccagtttat cttcaggaat accgattcca gtatctttta cctcaataca
     7501 aattttcgca tcttgcataa aacaattcag ctgaatattg ccggattcgg tatattttat
     7561 agcattatca agcagattat tgataatttg agtcaggcgg gtttgatcgc acaagattaa
     7621 ctttggtgta tccggatgaa tatctatcgt atactgaatt gctttctgac ccactctgtt
     7681 ctcaaaatat gtctcactgg cataaatgag tttatacaaa tcaaagttag ctttgaacgt
     7741 atgcaaacgg cctgtttcca aattggaaat atccagcaaa tcattaatga tagaagtgag
     7801 ctgattggca ttttccacac atttatttaa atagacttta ctgttttcat cttgttcatt
     7861 tttttcaaca atattcaata atcctttgat aatactgata ggattgcgca tttcgtggct
     7921 tatattggca atgaatatat ctttagaatt ttgcgattcg agcaattcta ttttagtctg
     7981 ttccagttca agattttttt gtgtcaaaat tttttctgct ttatccctgg ataaataaaa
     8041 gaagaaggag aataaaaaaa tcaataaatc caagccttca taaacaaaca gattaaacaa
     8101 agaaatacgg tattccacat cgcttaaacc tcgctgataa tttaccagcc aaaaaatgat
     8161 attgaccgtc agtgctaaaa aaagccagcg tattgcatct ttgtctttag caatcaaaag
     8221 cggaacaatc ggaatataaa cagtccaggt cattactgag gagtttatac cgccaaattg
     8281 gaatgcattg aaatatacat ttattactcc acctaaaaca aatagattgg ccaaaatcct
     8341 gtaattataa tttcttttga gtagcgtggg gagtaacaaa cagaagatgc cataaagact
     8401 gcactctacc atactccaat aattatgcat gataaatccg gaaacaaaag cgtataaaaa
     8461 agtaaaaaat gcagtgagct ttgagaaaaa tatcagtgtt tcccttttgt ctttaccttg
     8521 ggagataagc ctgtcaataa ttgattctat ataactattt cgccttccca ctatttctga
     8581 ataaataaaa taccagtttc tttaccagtg aaccgtctga aaaaggaacc cagagcatac
     8641 ccttactata atttttttca tccattctta tatgcgatct gaaaaataaa tatcctgaaa
     8701 aataaataat tattacgagg aaaacgacta taaaaaacag agaataaaaa tagcttatgc
     8761 aaagtaaact aaagacaaca cttgcaatag aaaaaatcaa tatcttcttt tccggtttag
     8821 tctgcaagtg tttgaataaa ataattaaaa caaatgctgc ggaaatatat ctgagaacta
     8881 acgaaacttt gcaaattatt ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnaacaaat gctgcggaaa tatatctgag aactaacgaa actttgcaaa ttattttgtg
     9061 caaaggagtt cctgctatta tagcatcttt gagtatgggc agcgaaggta agtgaggaaa
     9121 aatattatgc ttcgctatgt attcaaatat tagtaacacc gtggcaaagc cggcaatcaa
     9181 aaacaagcta aagaaaatga taaacaacgg tgtctttaag aacttgtaaa atgggttgat
     9241 atacttcgca atcttagaaa gttgtaattt gtcatattct ccgctatcat tatacaaatc
     9301 agatcctttc actgtttgaa caattgcatt tgtggcacgt atccccgatt cgcatgcgcc
     9361 ttccatagtg gccaaatcca cttcggtacg cacgtaatct ccggcaagaa acatgttcgg
     9421 tatacgggta tatgttgaag gtctattgca atacatattt acataattca ccaataattt
     9481 actttcattt tcgcatacaa ataccttttt cttaggtttg ccccgatgca aattcagcgg
     9541 attgtctgcg ccatcatttc ttactttttt ataatcggca tatgctgtgt cgaattctga
     9601 atgacggttt gtttccgtaa gttccaaata atcatttatc tcttgtaatg cttcacttct
     9661 tctttccgtt acagattcat caagaaaggt atattcgtaa atgatatcgt gatctgtggg
     9721 acaacggtag ccgcagattt caccattatc atctcgttct acaataaaag tcgctgcttt
     9781 aatttgctcc cacgcttctt taataatatc ttctctcgtc atttcaaata cagaacgctt
     9841 atcgcaaagg tcgctgtctt cgtcacagtt tgacataatt aatgataaaa tgccttttac
     9901 gtcgccattg ccaaacctct caggttgata cgcagcagtc caaaattgtt cttgatgaat
     9961 acccgttatc gcccattttg aattattcaa attaatatgg ccatgcgtaa tttcaacttt
    10021 gttccttaaa taaaaaacaa tgccacacat ccagtctaca ctacctttca aatgctggat
    10081 aaacttgagg gatgggtctg catgaaaaat atctgcatct ctcgaatcag tcaataattt
    10141 tgccattttt tcaaccggaa ttgcaaacaa gaaaatagct ttcatatcag cattatgctt
    10201 ccatgtataa gtttcatctt gcttaattcg tatgctctct atataataaa aatctttact
    10261 cttatttttg gataaggcag atttctcaac gtctacagtt ggcaattcct gatgataggt
    10321 acactctata tccacgcata cagcattcat ttcaaatttc accccttctt tttcaagttg
    10381 atttttccaa ggcaatagcc aagcctgatt cgtaggcgca ttgagtactc ttcctgcggc
    10441 acctcccggt gcaatcactt caaataacat cttaggcaat atcgtactac cggtacgcgt
    10501 gctggttttt ctgctctttg ccgcagcaag actgcgtgtc agtccatcag caaaaaaagt
    10561 tgcataatca ttaccgtaag aggtgttgcg tgcattaata aaatcaaacc aggactcgcg
    10621 ttcaaattct tcctgcttcc taaaatgtga tgaagtaatc attttaaaca atgcatacgc
    10681 aaaatgattt tttccattat gattaatatc caaataagca tctcgtgtgg aacgtatcat
    10741 tttcaaaaca gatccgaaat aatcccattg ctcggtctta ttcagtaata aaatgggatt
    10801 tctgttttgg tttttgaaga acgaaaacat atacgtactg gtttgtacca gattatcaaa
    10861 tacactttta ttttttttat ttcccggatt atctgcggga atttctttta atacttgatc
    10921 gatattggaa taaaaacccg gaaaaaaacg aaagccatgt tcagccggaa ggaaattaag
    10981 tctgttacta tcttgaaaat tattttccag atgttcaata ctccttgctt tgcctccaat
    11041 atatttagga tctttttcaa atagagtaat attaaaaacc ttttttccgt ctattttctt
    11101 tttactcaat tcgtgtgcgg ctgtcatacc ggccacacct cctccgataa tataaatgtt
    11161 tatcagttta tttattgcca taattttatg tttttattta aataaatata gtatatttac
    11221 tccataaacc gtatggctgg agaaataaaa aaccggatta ttgacttttt aaaagatttt
    11281 gaaacctctc gcaaggagcg atatatttta ggtattgcag atttttcaga gaaattgagt
    11341 cagcttacca tagccttaaa agatgaagaa gaaggtcaaa tccgatttct tctgcattcg
    11401 ttgaaaccat taagccagct gctgaattta tctgaattaa acttactttt gcattccatc
    11461 tctaaagacc ataacactga ttattcagaa attatacttc tcctggaaag taaaatacaa
    11521 gaaattgaca ctaccttaaa cagtcttcat tagcaacttc ttgtattagc caataatatt
    11581 ttacatttgc accatgattt tggtaactgg ggcatccgga ttcttgggcg gtgaattggt
    11641 aaaacaacta gtatttcaag gtgaacaagt gcgtattatt aaacgtaagc attccgattt
    11701 atcgcattta caggctttcc aaaacaaact ggaaattaca gagggtgata ttttagatat
    11761 tccatcctta gaaacagcct gtgaaggcat agataaaatt tatcatgcag ctgcagttat
    11821 tggttacgat gcctcttact atgacaagat gtataagaca aatattgaag gtacggcgaa
    11881 cgtagtcaat attgccttgt ataaaaatgt aaagaaaatt gtacatatca gctctgtggc
    11941 tgccattggc ggtaaacctg atgtcgaaat tgatgaggaa accaaatggg aaaaaaacga
    12001 atggactaca cattatggca tcacgaagat gctggcagaa cgagaaatct ggcggggtgt
    12061 gcaagaaggg ctggatgccg taattgtcaa tcccggcatt atcattggtg tgggcaatga
    12121 tgaacataaa gctacactaa gattattcag acagatagca acacgcaaat tgccatttta
    12181 cactccgggt tctatcggct tcattgatgt agaagattgt gcacgtgctt gtattgattt
    12241 aatggaatgc aaaaaaacgg ctgagcgttt tatcttagtg aacgaaacac tcacttacaa
    12301 aaactatttt gaaaaaattg cacaggcatt gcaagtagca cctccacaga tgaaattaaa
    12361 cagatattca ggaaggtttt ttgtattctt agatgcgatg ttgagtacat tttcttctcg
    12421 caagcgcggg cttacagctg aaaatcttaa aattgccatg gaaaattttc actataaaaa
    12481 tgacaaattt gtcaatcagt tttcatttac atacaccccg atggatgtca ctatcaataa
    12541 tattgcaaaa cacataaaac aataagatgg attttttacg aaaagaaaat aaaaataaaa
    12601 tcttaggtat atggtatatg gtgggtgtcg gtatgctgtt gggtcaaatt ttattaggcg
    12661 gcatcacccg gcttacaggc agcgggcttt ctattacgga gtggaaggct atactgggat
    12721 ttattcctcc gatgaatgaa caagcctgga atttggcatt tatcaaatat cagcagttcg
    12781 gacaatttaa attggtcaac acatcgatga cacttgaaca gtttaaacac atctacttct
    12841 gggaatggtt tcatcgttta tggggacgac ttattggcat tgcagcactt atcccattaa
    12901 tcatttttgc aataaagagg ttagtaaaca aacaggatat tttcaaaatt gtcattgcaa
    12961 tagtgttagg tggaatagcc ggaatagtgg gctggattat ggtgcaaagc ggactcaaac
    13021 aaaatacggt attggtgaat cctgtcaacc tgatgtcgca tatagtagtg gccacaataa
    13081 ttgtagctta tgtattgtac accggtttgg gttatttata tcccaataga gataaaaacg
    13141 ataacaagaa taagcgcatt tattttctgc tattgatact acttgtgttt atacagattg
    13201 cactgggagc attagtggcg ggg
//
LOCUS       DAOQII010000041        12985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2346_length_12985_cov_4.714462, whole genome shotgun sequence.
ACCESSION   DAOQII010000041 DAOQII010000000
VERSION     DAOQII010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2346_length_12985_cov_4.714462"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLF48_03305"
     CDS             <1..333
                     /locus_tag="PLF48_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48383.1"
                     /translation="TTGTYGFIFHFMFLCNFVLPFFILMKRKAKRNPKVTNVVACIII
                     FGQFTWFFLMNCPARIPIGGYGLISIGLLFMFGSIFIWVTLKALSKIKDLASTTHPFV
                     EESYKLHI"
     gene            701..2230
                     /locus_tag="PLF48_03310"
     CDS             701..2230
                     /locus_tag="PLF48_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF48384.1"
                     /translation="MSSFILGLLSLILFYIVIVQIAKAKDLVKALQDGQEEEASHGKR
                     AFYLLLLGFAILAYSIISAFVYKKYLLPKPATEHGAWIQQLINITLIFTGSVFVITQA
                     LLFYFTYKYHFKKGNKAYFYPHNNTIEVVWTVIPAIVLTVLIGIGMWRWYSIFNVFKD
                     RPRDVCEVEVIGKQFMWVLRYPGKDGVLGKRDFSLVCPDNELGINWNDPASHDDILAD
                     EMVLPKGKTVYANIGSLDVIHSFYIPEFRFMMDAVPGVPTKFWFKPTITSEEMKKIKN
                     DPEFSYYIACNNICGNGHYNMKKKVRVVEQAEYDKWLGEQKSYYETVAKAAIASGSII
                     LPGQGSNTNAAPAEEAPSTITSSTIAYSNTLSTGFALSGAVANGVENKLIDFIKSGEA
                     VSKDKWFSFDRLLFETGKSTLKPESQEQLKNVAEIMKAFPSVEIKIGGYTDNVGDPAA
                     NMKLSDDRAKNVMAELVKLGVPENRMKAEGYGEQYPVADNKKAEGRQQNRRIDIRVTK
                     K"
     gene            2265..4079
                     /locus_tag="PLF48_03315"
     CDS             2265..4079
                     /locus_tag="PLF48_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF48385.1"
                     /translation="METLHTGTEQQVDVHAHGHGEHHHQETFITKYVFSLDHKTIGKQ
                     FLITGMAWAIIGGLMSVIFRLQLGWPDETFPWLERIFSAWYTDGKLTPEAYYSLITMH
                     GTIMVFFVLTASLSGTFANILIPLQIGARDMASPLMNAISYWLFFISGVIMFASLFLP
                     TGAASAGWTIYPPLSALPQASPGSGAGMTWWLIAMVLFVASTLVGGLNYVSTVLNLRT
                     KGMTMFKLPLTVWALFFTAIIGILSFPVLVSGVVLLIFDRTLHTSFYLSDIVIGGKAM
                     AFAGGSPVLFQHLFWFLGHPEVYIIILPAMGMVSEILAVHSRKPIFGYKAMIFSILGI
                     TILGFIVWAHHMFVAGVNPFLGSIFTLFTLLIAIPSAVKVFNWLTTLWKGNIKITPPL
                     VFAVAFVSTFISGGLTGIWLGNSTIDIQVHATYFVVAHFHIVMGVSAFFGMFAGVYHW
                     YPRMFGRLMNETLAYIHFAITFIGAYLIFWPMHFQGLAGVPRRYFTNSEFPQFAVFDD
                     MNKLISIATVIVFFAQFIFLINFFWSIKRGRKSGQNPWGANTLEWTSPIEGIHGNWEG
                     DLPTVYRWPYDYSRPGAETDFIPQTVPLTEKELQEEIH"
     gene            4107..4997
                     /gene="cyoE"
                     /locus_tag="PLF48_03320"
     CDS             4107..4997
                     /gene="cyoE"
                     /locus_tag="PLF48_03320"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNF48386.1"
                     /translation="MQNTASISFMTSIKLKIRDIMELTKFRLSFLVVFSAVMAYLMAA
                     DSYTKISVLLLSLGGFLVTASSNTINQIIEKDLDKLMSRTQNRPLAAGRMKLVEAYII
                     AGICGVSGIFILGFYFNALSGLLGALALLSYAFIYTPFKRISPMAVFIGAIPGSLPLL
                     IGYTAATGTIGLEGLLLFAIQFFWQIPHFWAIAWLLDDDYKKAGYFLLPSSDGKTRKA
                     ALQNIPFLLGLIVMSILPYYFKFSGITSLIIVLIMGFYFLTAGIQLATDLNDKSAKKL
                     MFASFIYLPVVFIALVLDKL"
     gene            4997..5626
                     /locus_tag="PLF48_03325"
     CDS             4997..5626
                     /locus_tag="PLF48_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNF48387.1"
                     /translation="MGVTASYQRQNNVQGAQRFTMWLAILSVMMIFAGFTSAFVVRKG
                     AAGNWSSFLMPKIFNVATFVILLSSAFLYVAHTANKKGNKKLMATGLWITFGLGILFC
                     ILQIMGWGALYNQGVYLAFSPNPAGSFFYVITFMHFIHVGGGLIFLLIASIHALIQLK
                     TTDKTILIELEDKEKGIYSLRTDLLSMYWHFMGLLWLYLFIFLYLNFNH"
     gene            5644..6480
                     /locus_tag="PLF48_03330"
     CDS             5644..6480
                     /locus_tag="PLF48_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNF48388.1"
                     /translation="MSEVLEVQKHHVDEHLPAPTPDNGEGGEKSPMGISYGKTMMWYF
                     LLSDAFTFSAFLIAYFSLRLSHKWWPDPNHVFNAMPFMGHHQIPMGFVSIMTFILIMS
                     SVFIVRAVQEGHRMNQKGVMFWMFWGIVGGAAFLGCQAWEWTHLLGEGMRPFFNPFVP
                     DVNGITHLAENHFDAGIGQRVIDLVGNTFTSHGEKFQLMDGFRATGGPVAFGALFFGI
                     TGFHGFHVFTGVCLNIWGFFATGAGVFQRRGHYEMIEKLGLYWHFVDLVWVFVFLSFY
                     LM"
     gene            6522..6869
                     /locus_tag="PLF48_03335"
     CDS             6522..6869
                     /locus_tag="PLF48_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNF48389.1"
                     /translation="MSAEHLTDEEYKSQVSAVWRVTVVLSVITVLEVAVALAHYYGFL
                     PHFIGRFLLNTFYALLSILKAAYIIGEFMHLKYEKRALMLSLGFPLVFLVWAIIALLY
                     EGNALHKALYMIQ"
     gene            6878..7591
                     /locus_tag="PLF48_03340"
     CDS             6878..7591
                     /locus_tag="PLF48_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNF48390.1"
                     /translation="MNSKNKKGFFLVFILVAVFPVAFLFIFMNIEKHSPKVDLNRILP
                     IYGPKDTFTNFTRNGKPFLDTAYFQIPNFSFINQNGDTITKERVMGHVLIVDFFFTTC
                     KSICIDMHRNMQTVQDAFLKENEVLILSHTVDPETDSVGQLFKYGIEHQVNSKIWNLL
                     TGNKQDIYKMARNGYFITATQGTGGPEDFIHDQKFCLIDKEGRIRGYYNGTDPEQVKK
                     LIDATKALLASYHIPLRAK"
     gene            complement(7805..8137)
                     /locus_tag="PLF48_03345"
     CDS             complement(7805..8137)
                     /locus_tag="PLF48_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48391.1"
                     /translation="MRTLSLCISLFLLFTHCNSTPSDDIPSITIDDIHYNLRAVVNGV
                     IPTENYKIKACSSFIIDATKYDFSILKKMNNGNLPDMIQITTKNGTFAVIWILKMKLL
                     LMKNLRLI"
     gene            complement(8151..10700)
                     /locus_tag="PLF48_03350"
     CDS             complement(8151..10700)
                     /locus_tag="PLF48_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48392.1"
                     /translation="MMLKRTLFCCFLLLSLCTLAQTRLVVKGKITNINGDGVSEVNII
                     QNGTKNTTTSNSKGYYNIEVEYEDSCILEFSHVEYGKKYITLLPTNKASVYKDIHFES
                     AVNTIDTVEITAKKNDVGISTIDAQDVKYLPNPTNDMGTVVKTIGQGVQSNNELTSQY
                     NVRGGNYDENLVYVNDFEIYRPLLISNSAQEGLSFANLALTDKLSFSGGGFESKYGDK
                     MSSVLNIKYKTPTAFHGSFSASLLGVDAHVEGTSKRKKDSSNVLPKFTYLIGARYKSN
                     AYMLGALDKDGEYNPNFFDIQGDFHIRPSLKHDIELIGNHAFNNYKFVPVSEETRAGT
                     FDQLIRFLVEFEGLEKNRFQNSMAGIAYKYLPNDKLSLKFMSSFWKLKESESYNVLGY
                     YSLDEIEIDPSSDNFGNVKANLGVGTFHNWARNTLDAIVTNFSHSGKYSLRSKKDNTE
                     KHLFEWGATVQFEKINDKLSEWTRIDSSGYSIPYTGSSIQFPYRLKSSASLSSIRTFG
                     YFQDTWTIFKRDSSYLTLNTGLRYTFWSVNKQFLVSPRVQLIYKPKLKSDVTFRLAGG
                     MYVQPPFYREIRDFDGIIHRNVKAQESYHVIAGAEYNFKIKKRPFKFTAEAYYKYIRH
                     LNPYEIQDVRIRYFANNDAKGYATGFDLRLFGEMIKGIDSWVTISYLNTKENLNNDYY
                     YSYQDSTGAIYSNPSKTTNPITDTLTNYPGFIRRPSDQALFVSLFFQDYLEKYKRFKV
                     HLNLVIGTGLPFGPPDRNRYKDVLKMPPYRRLDIGFSALLLSGEKRGEKKPNSFGSHF
                     DKIWASFEVFNILGIRNTLSYRWIKDTENNLWAIPNYLTSRRFNVKMQITW"
     gene            complement(10718..11368)
                     /gene="rpe"
                     /locus_tag="PLF48_03355"
     CDS             complement(10718..11368)
                     /gene="rpe"
                     /locus_tag="PLF48_03355"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNF48393.1"
                     /translation="MPLIAPSILSADFSNLQSEFDMLNTSRADYIHIDVMDGVFVPNI
                     TLGFPVIKSMRKLSTKTFDVHLMIVEPDRYIEDFKKAGADILSVHIEACTHLHRVIQK
                     IKQSGMQAGVAVNPHTSISLLENIISDIDIVCLMSVNPGFGGQSFIEQTYSKISRLKE
                     LIIQKNSAALIEIDGGVTLDNASKLIAQGADVLVAGNTVFTSSDPIKTIEQLKNIN"
     gene            complement(11518..12711)
                     /gene="sucC"
                     /locus_tag="PLF48_03360"
     CDS             complement(11518..12711)
                     /gene="sucC"
                     /locus_tag="PLF48_03360"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNF48394.1"
                     /translation="MNLHEYQGKELLKQFGIKVQEGIVAETVDQAIDAYKTIIARTGR
                     DGVVVKAQIHAGGRGKGGGVKFAPDFDALKEKAQAILGMTLVTPQTGPQGKVVHKILI
                     AEDAYYEGASPTKEFYMSVLLDRAKECNAIVYSAEGGMDIEEVAENHPEKIFKEWADP
                     AVGIQPFQARRIAFNLGLSGAAFKDMVDFVQKLYKAYVGSDASLFEINPVLKTSDDKI
                     IAVDAKVNLDDNALYRHPDLEAQNDKLEIDPTELEATEADLNYVQLDGNVGCMVNGAG
                     LAMATMDIIKLSGGEPANFLDVGGTANAERVEKAFRIILKDKKVKAILINIFGGIVRC
                     DRVAEGVIDAYKSIGNIEIPIIVRLQGTNAEIAKKMIEESGLKVQSAILLSEAADLVR
                     LAVAE"
BASE COUNT         3873 a         2265 c         2408 g         4439 t
ORIGIN      
        1 acaactggca cttatggttt tatcttccac tttatgttct tgtgtaactt cgtattgcct
       61 ttctttatat taatgaaacg caaagcaaaa agaaacccaa aagtaaccaa tgtagttgca
      121 tgtattatca tttttggtca gtttacctgg ttcttcttaa tgaactgtcc ggctagaatt
      181 ccaataggtg gttatggttt aattagcatc ggattattat ttatgttcgg cagtatcttt
      241 atttgggtga cattaaaggc tctttctaaa ataaaagatc tagcttctac cactcatcca
      301 tttgtggaag aaagttataa actgcacatt taaaacacac atcatttttt ataaaagacc
      361 tgatttattc aggtcttttt ttgttctttt tctagattac ctaatattag gtagtttcgc
      421 taaagcacta acaaatactg tatgactgag taaattatca gactcataaa aattattaac
      481 tattgtgtgt agtgtgcact aaaaaaaatg ataattctta tcgaagaatt agtttttttt
      541 aatagatttg caagaattta caacgtatag cattccagaa aataacaagc acgattaaga
      601 ttcaattgct aataaataat tttgattgtt ggattcgatg tttacttgta aattaaaaaa
      661 attccttact gataaaacat taaataaaac ttaggtatct atgtcatcat tcattttagg
      721 attattatcc cttatcttat tttacatagt aatcgtacag atagcgaaag caaaagacct
      781 tgtaaaagca cttcaagatg gacaagaaga agaagccagt catggtaaaa gagcatttta
      841 tttattactg ctcggatttg caattttagc atattccata atttctgcat tcgtgtacaa
      901 aaaatatttg ttaccaaaac cggctacaga acatggtgca tggattcaac aattgataaa
      961 cataactttg atatttacag gctctgtatt tgtcattaca caggctttgc ttttttattt
     1021 tacctataaa tatcacttta agaaaggcaa taaagcttat ttctatccgc ataataatac
     1081 gattgaagta gtttggacgg ttattccggc tatcgttttg actgtattaa ttggaattgg
     1141 catgtggaga tggtatagta tcttcaatgt atttaaagac agaccgcgtg atgtatgtga
     1201 agtagaagtc attggcaaac aatttatgtg ggtattgcga tatccgggta aagacggagt
     1261 attgggtaaa cgtgattttt ctttagtttg tcctgataat gagctaggaa taaactggaa
     1321 tgatcctgcc agtcatgatg atattttagc agacgaaatg gtattgccga aaggaaaaac
     1381 ggtgtatgca aatattggtt ctttagatgt aattcatagt ttttatattc ctgaattccg
     1441 tttcatgatg gatgctgttc caggtgtacc aactaaattt tggttcaaac cgacaatcac
     1501 atctgaggag atgaaaaaaa ttaaaaatga ccctgaattt agttactata tagcttgtaa
     1561 taatatttgt ggtaacggtc attataatat gaagaaaaaa gttagagtag ttgaacaagc
     1621 agaatatgac aaatggttgg gtgagcaaaa atcatattac gaaactgtgg caaaagcagc
     1681 catagctagt ggttcaatta ttttgccggg acaaggaagt aatacgaatg cagctcccgc
     1741 tgaggaagcg ccttccacta taacaagctc tacaatcgct tatagtaata cactttctac
     1801 aggttttgca ctttcgggtg ctgttgcaaa tggcgttgaa aataaattga ttgattttat
     1861 caaaagcggt gaagctgttt caaaagataa gtggttttca tttgaccgat tattatttga
     1921 aacaggaaaa tcaactttaa aaccggaatc gcaggaacaa ttgaaaaatg ttgcagaaat
     1981 catgaaagct tttccaagcg tagaaattaa aatcggtggc tatacagata atgttggtga
     2041 tccggctgcg aatatgaaac tatccgatga cagagctaaa aatgtaatgg ctgaattagt
     2101 aaaattaggt gtacctgaaa atcgcatgaa agcggaagga tatggcgaac aatatcctgt
     2161 tgcagacaat aaaaaagcag aaggtcgtca acaaaacaga agaatagaca ttcgtgttac
     2221 taaaaaataa ttttcaactc caaatctttt gtaaataatc atatatggaa acattgcaca
     2281 caggtactga acagcaggta gatgtacatg ctcatggaca tggagaacat caccatcagg
     2341 aaacatttat cactaagtat gttttcagtt tagaccataa aacaattgga aagcaatttt
     2401 tgataaccgg tatggcatgg gccataattg gtggtttgat gtctgttatt ttccgtttgc
     2461 agttaggctg gccggatgaa actttccctt ggctggaacg cattttttct gcttggtata
     2521 cggatggaaa attaacgcct gaagcctact attcattgat aaccatgcat ggtaccatta
     2581 tggtattttt tgtattgaca gcgagcttga gcggtacatt tgccaatatc ttgattccat
     2641 tgcaaatagg agctcgtgat atggcatctc ctttaatgaa tgcaatctca tattggttgt
     2701 tttttatctc aggagtaatc atgtttgctt ctttattttt gcctaccggt gctgcatcag
     2761 caggttggac gatttatccg cctttaagtg cattgcctca agcatcaccg ggttcgggtg
     2821 caggtatgac atggtggttg attgcaatgg tattattcgt agcatctaca ctcgttggcg
     2881 gtcttaacta tgtatctacc gtgctaaatt tgcgtacgaa aggaatgacc atgtttaaac
     2941 tgccacttac agtatgggca ttgtttttta cagccatcat cggtattcta tctttccctg
     3001 tattggtttc cggtgttgta ttattaattt tcgacagaac cttacatact agtttttatt
     3061 tatcagatat cgtgataggt ggtaaagcaa tggcctttgc aggtggttct cccgtgttat
     3121 tccagcactt attctggttc ttggggcatc ctgaagtata tatcatcatt ttaccagcca
     3181 tgggtatggt ttcagaaata ttggccgtac attctcgtaa gccaatattc ggttataaag
     3241 caatgatatt ctctattttg ggtattacaa ttttaggctt catcgtatgg gctcaccaca
     3301 tgtttgtagc tggtgtaaat ccgtttttag ggtctatatt taccttattt actttgttaa
     3361 tcgcaattcc atctgcagta aaagtattca actggttaac tacactttgg aaaggaaata
     3421 ttaagattac tccaccatta gtatttgcag ttgcctttgt ttctacattt atctccggtg
     3481 gattaactgg tatttggttg ggtaactcta ctattgatat tcaggtacat gctacttact
     3541 ttgtcgtagc tcacttccat attgtaatgg gtgtttcagc attctttggt atgtttgccg
     3601 gagtatatca ctggtatccg agaatgtttg gtcgcttgat gaatgaaaca ttggcataca
     3661 tccactttgc gattacattt atcggagctt atcttatctt ctggcctatg cactttcaag
     3721 gtttggccgg tgtaccgcgc agatacttta ctaatagtga gttcccgcaa tttgctgttt
     3781 ttgatgacat gaataaatta atcagtatcg ctactgttat tgtgtttttt gcacaattta
     3841 tattcttgat taacttcttc tggagtataa aacgcggtag aaaatcagga caaaatccat
     3901 ggggtgccaa tacgttggaa tggacttcac caattgaagg tatacacggt aactgggaag
     3961 gtgacttacc taccgtatat cgctggcctt atgattacag cagacctgga gcagaaactg
     4021 attttattcc gcaaactgtc ccattaactg aaaaagaact acaagaagaa atacattaaa
     4081 aaaatattat tttaattaac gtatttatgc aaaatacagc ctccatatct ttcatgactt
     4141 caataaaatt gaagatcaga gatattatgg agctgacaaa attcagatta tcttttttag
     4201 tcgttttttc tgcagtaatg gcctatttaa tggcagctga cagctatact aaaatatctg
     4261 ttttattatt gtcactaggt gggtttttgg ttacagcatc gtccaataca ataaatcaaa
     4321 ttattgaaaa ggacttagat aaattgatgt cgcgtacaca aaacagacca cttgctgcag
     4381 gcagaatgaa gttagttgaa gcttacatta ttgccggtat atgtggggta agtggaattt
     4441 ttattcttgg attttacttt aatgcactaa gtggattgtt aggtgctctg gcattattat
     4501 cctatgcatt tatttatacg ccattcaaac gtataagtcc tatggcggta tttattggag
     4561 ctatacccgg ttcattgcct ttgttgattg gctatactgc cgcaaccggc actatcggtc
     4621 tagaaggtct tttgcttttt gccatacaat tcttctggca aattccgcat ttttgggcga
     4681 tagcatggtt attagatgat gactataaaa aagcaggcta tttcttattg ccatcatctg
     4741 atggaaaaac aagaaaagca gctttacaaa atataccgtt tttgttagga ttaattgtaa
     4801 tgagtattct accttattat tttaaattta gcgggataac ctcattaatt atagttctca
     4861 tcatgggatt ttattttctc actgcaggaa tacaactggc aacagactta aatgataaaa
     4921 gtgcaaagaa actaatgttt gcttctttta tttacttacc tgtcgtattc attgctttag
     4981 ttttagataa gttataatgg gagtaacagc atcatatcag cgtcaaaata atgtacaggg
     5041 tgcacaacgt ttcacgatgt ggctggctat attgtctgtg atgatgatat ttgccggatt
     5101 tacaagcgca tttgtggtgc gtaaaggtgc agccggaaat tggagtagct ttttgatgcc
     5161 caaaattttt aatgtagcta catttgttat tttgctgagt agcgcatttt tatatgttgc
     5221 acatactgca aataaaaagg gtaataaaaa actgatggca accggacttt ggattacttt
     5281 tggtttgggc atcttatttt gcatactgca aataatgggt tggggtgctt tatataatca
     5341 aggtgtttat ttagcattca gtcctaatcc tgccggctcg tttttttatg tcattacatt
     5401 tatgcatttt atacatgttg gcggtggctt gatattttta ctcatagcct ccatacatgc
     5461 attaatacaa ttgaaaacga cagataagac tatattgata gaattggaag acaaagaaaa
     5521 aggtatttac agcctgcgta ctgatttgct ttctatgtat tggcatttta tgggcttgct
     5581 gtggttgtat ttgtttatat ttttatactt aaattttaat cattaatcga ataaattcat
     5641 tctatgtcag aagtactgga agttcaaaag catcatgttg acgagcattt acccgcacca
     5701 acaccggata atggtgaagg cggggaaaag tcgcccatgg gaataagcta tggaaaaacc
     5761 atgatgtggt attttttatt gtcagacgca tttacatttt ctgcgttttt gatagcttat
     5821 ttctctttgc gcttatcgca caaatggtgg ccggatccta atcatgtatt taatgctatg
     5881 ccatttatgg ggcatcatca aataccaatg ggttttgtat ctatcatgac ctttatcttg
     5941 attatgagtt ctgtttttat tgtacgtgca gtacaagaag gccaccgaat gaatcaaaaa
     6001 ggtgtaatgt tctggatgtt ttggggaata gtaggtggtg cagctttctt aggttgtcag
     6061 gcttgggagt ggacacattt attaggagag ggtatgagac ctttctttaa tccttttgtg
     6121 cctgatgtta atggtattac acatttggca gagaatcact ttgatgccgg tatcggacaa
     6181 agggtaattg atttagtagg aaatacattt acttcacatg gtgagaaatt tcaacttatg
     6241 gatggcttta gagctactgg tggtccggtt gcatttggtg ccttgttctt cggcattaca
     6301 ggttttcacg gtttccacgt attcacaggg gtatgtttga atatttgggg attcttcgct
     6361 acgggtgcag gggtatttca acgcagagga cactatgaaa tgattgaaaa attgggttta
     6421 tattggcact ttgtagattt ggtttgggta ttcgtattct tatcattcta tctaatgtaa
     6481 taaccttaaa acattatata aaaatatata aaataaatac tatgtcagca gagcatttaa
     6541 ccgacgagga atataaatca caagtatcag cagtatggcg agttacagtc gtactttctg
     6601 tgattacagt tttggaagta gctgtcgcct tagcacacta ttatggcttc ctaccccatt
     6661 tcataggtag atttttactc aatacctttt acgctttact ttccatactg aaagctgcat
     6721 acatcattgg agagtttatg cacttgaaat atgaaaagcg tgcgttgatg ctctctttag
     6781 gttttccttt agtgttttta gtatgggcaa ttattgcctt attgtacgaa ggcaatgctt
     6841 tgcataaagc attgtacatg attcaataat tgaatccatg aattctaaga ataaaaaggg
     6901 atttttttta gtttttattt tagtggctgt atttccggtt gcattcttgt tcatttttat
     6961 gaatatcgag aagcattcgc caaaagtaga cttgaacagg atactgccta tttacgggcc
     7021 aaaagatacc tttaccaatt ttacccgtaa tggtaaacca tttctggata cggcttattt
     7081 tcagattcct aatttctctt tcatcaatca aaatggtgat accattacaa aagaacgtgt
     7141 aatggggcac gtgctgattg ttgatttctt ttttactact tgcaaatcta tctgtattga
     7201 tatgcaccgc aatatgcaaa cagtacaaga tgcattttta aaagaaaatg aagttttgat
     7261 tttatcgcat acggttgatc cggaaactga ctcagttggg caattgttta aatatggaat
     7321 tgagcatcag gtaaattcaa aaatatggaa tttactaacc ggcaataaac aagatattta
     7381 taagatggcc cgcaatggct attttatcac tgctacacaa ggaacaggcg gccctgaaga
     7441 ttttatacac gaccaaaagt tttgtcttat tgataaagag ggtagaataa gaggttacta
     7501 taatggcacc gatccggaac aagtgaaaaa attaatagat gctacaaaag ccttgttagc
     7561 cagttatcat attccgctta gagcaaaata gtttttctag catttacatt attggataaa
     7621 aaagatttgg agaaatttag gaagttgcgc aattttcttc tgaatttatt tccatattat
     7681 tcaacttcaa tgaaagttgt ataaaacgat ttgaattcta cattttcatt tactgatagc
     7741 aacgttccga tacttatcaa tgctttttct tttggttgta aacctgagaa aacttcatga
     7801 ccacctatat taatcgcaga tttttcatca acagtaattt cattttcagt atccaaatca
     7861 ccgcaaacgt tccgttcttt gttgttattt gtatcatgtc gggtaaattt ccattgttca
     7921 tcttttttaa aatggaaaaa tcatatttcg ttgcatcaat aataaatgaa ctgcacgctt
     7981 ttattttata attttccgtt ggaataacac cattcacgac agcacgcaaa ttataatgta
     8041 tgtcgtcaat ggttatgctt ggaatatcgt cagatggagt agagttacaa tgcgtaaaca
     8101 acaaaaacag gctaatgcat aatgacagag ttctcatgat ttgtttatgc ttaccacgta
     8161 atttgcattt ttacattaaa tctgcgtgag gtgaggtaat ttggtatcgc ccataaatta
     8221 ttttcggtat cttttatcca tctgtaagaa agcgtatttc taattcccag gatattaaag
     8281 acttcaaaac tcgcccaaat tttatcaaaa tgactgccga aagagttggg ctttttctcc
     8341 ccgcgttttt caccactcaa taataaggca gaaaaaccaa tatccaagcg tcggtaaggc
     8401 ggcattttta aaacatcttt atagcgattt ctatcgggag gaccaaatgg caaacctgtg
     8461 ccgataacca aattcaaatg cactttaaag cgtttgtatt tctccaaata atcctgaaaa
     8521 aacaagctga caaacaatgc ctgatcgctg gggcggcgta taaatccggg ataattagtt
     8581 aacgtatcgg tgattggatt agtagttttt gacggattac tgtagatagc tccggtactg
     8641 tcttgataag aataataata gtcgttattc agattttctt tcgtattcaa ataggaaata
     8701 gtcacccacg agtcaatgcc ttttatcatt tcgccaaaca agcgcaaatc aaaacctgtg
     8761 gcataacctt tcgcatcatt attggcaaaa tagcggatgc gtacatcttg tatttcatag
     8821 gggttcaaat gccggatata cttgtaatag gcttcagccg taaacttgaa aggtcttttt
     8881 ttgattttga aattgtattc agctccggca atcacatgat acgattcctg agccttgaca
     8941 ttccggtgaa tgatgccgtc aaaatcgcgg atttctctgt aaaatggcgg ttgtacatac
     9001 atgcccccgg ctaaacgaaa ggttacatca ctttttagtt tgggtttgta aatcagctgt
     9061 actcttggtg acactaaaaa ctgtttattg acagaccaaa atgtatatct caagcctgta
     9121 tttaaagtga gatagctgga gtcgcgtttg aaaatagtcc aagtatcctg aaaatagcca
     9181 aatgtgcgaa ttgaactcag cgaagcactt gattttaaac gataaggaaa ctgaatggaa
     9241 cttcccgtat agggaataga ataaccggat gaatcaatgc gtgtccattc acttaattta
     9301 tcgtttattt tttcaaattg taccgttgct ccccactcaa aaagatgttt ctcggtattg
     9361 tctttttttg atcttaatga atattttccg ctatggctaa agttcgttac tattgcatct
     9421 aaggtatttc ttgcccagtt gtgaaaagtg ccgacaccaa gattggcttt tacattaccg
     9481 aaattatcac tgcttggatc gatttcaatt tcatctaagc tgtaatatcc caatacatta
     9541 taactttcac tctctttcaa tttccaaaag gaagacatga attttaaact cagtttgtca
     9601 ttgggtaagt atttataagc aattcctgcc atagagtttt gaaatctgtt tttttccaaa
     9661 ccttcaaact caaccagaaa acgaatgagc tggtcaaatg ttcctgcgcg ggtttcttca
     9721 gaaactggaa cgaatttgta gttattaaaa gcgtggttgc ctattagttc aatatcgtgt
     9781 ttcaggctgg gacgaatatg aaaatcgcct tgtatgtcaa aaaagttggg attgtattca
     9841 ccgtctttat ctaatgcacc aagcatgtag gcatttgatt tatagcgtgc accaattaag
     9901 taggtaaact taggtaaaac attgctcgaa tcttttttgc gtttggatgt gccttctaca
     9961 tgggcatcta cacccaataa gctggcagaa aatgacccgt gaaatgcggt aggtgttttg
    10021 tatttgatat tgagtaccga tgacatttta tcaccatact ttgactcaaa gccaccaccg
    10081 gaaaaactca acttatcggt taaagctaaa ttggcaaaac tcaatccttc ctgtgcactg
    10141 ttggaaatga gcaatgggcg gtaaatttca aaatcgttga catacactaa attttcgtca
    10201 taattgccgc cacgtacatt gtactggctc gttagctcat tattcgattg aacaccttgt
    10261 ccgatagttt ttacaacagt acccatgtca ttcgtagggt tgggcaaata tttcacatct
    10321 tgcgcatcaa tggtactaat gcccacatca ttttttttgg ctgtaatctc aacagtgtca
    10381 atggtattaa cggcactttc aaaatggata tctttataaa cagatgcttt attagtgggt
    10441 aataaagtga tatacttttt gccatattcc acatgactga attccaatat gcaggaatct
    10501 tcatattcta cttcaatatt gtaataccct ttgctattag aggtggtcgt gtttttggtg
    10561 ccattttgta taatatttac ttcagatacc ccatctccat tgatattagt aattttgcct
    10621 tttacgacta atctggtttg tgccagtgta caaagtgaaa gtaaaagaaa gcagcaaaat
    10681 aaagtccgtt tcagcatcat actataaacg taagaaatta atttatattt ttaagctgtt
    10741 cgatcgtttt tatcggatcg gaagaagtaa aaaccgtgtt tccggctacc agtacatcag
    10801 caccctgtgc aattaatttt gaagcattat ccagcgtcac ccctccgtca atttctatta
    10861 atgccgcact gtttttttga ataatcaatt cttttaagcg actgattttt gaataggttt
    10921 gttctataaa tgactgcccg ccaaatccgg gatttacact cattaaacaa actatatcaa
    10981 tatcggaaat aatattttct aataaactta tagaagtatg tggattaacg gcaacacctg
    11041 cttgcatgcc gctttgcttt attttctgaa tcactctgtg caagtgtgta caagcttcga
    11101 tatgaacgga taaaatatct gcacctgctt ttttgaaatc ttcaatatag cggtctggtt
    11161 ctactatcat cagatggaca tcaaaggttt tcgtagaaag tttgcgcatc gattttatca
    11221 ccggaaatcc aagggtgata ttaggtacaa atacaccgtc catcacatca atatggatgt
    11281 aatctgcccg gctggtattc agcatatcaa attctgattg caaattagag aagtcagcag
    11341 ataatatgga tggcgcaatg agtggcaagt agtagagtta agtagtttat tagttgattg
    11401 gttaattgga tcatttgaaa gatatcacaa ttcaaaattc atcattcaac atttatcatt
    11461 caaaattcaa aatttataat tcaacattca acattcaaca ttcaacattc atcattctta
    11521 ttcagcaacc gccagtctta ctaaatctgc ggcttcgctt aataaaattg cagactgtac
    11581 tttcaatcct gattcttcaa tcattttttt agcaatttcg gcattcgtgc cttgcaaacg
    11641 aacgataatt ggtatttcaa tatttcctat ggatttatag gcatcaataa caccttctgc
    11701 tacacggtcg catcgcacaa tacctccgaa aatattaatt aagatggctt ttactttttt
    11761 atcttttaag atgatgcgaa atgctttttc tactcgctca gcatttgctg ttccgcccac
    11821 atccagaaag ttagccggtt caccgccact caatttgata atgtccattg tggccatagc
    11881 caatcctgca ccgtttacca tacagccaac attcccgtct aattgtacat aatttaaatc
    11941 agcttcagtg gcttccaatt cagtggggtc tatttccagt ttgtcatttt gtgcttccaa
    12001 atcaggatga cgataaagag cgttgtcgtc aagatttact ttagcatcaa cggcaataat
    12061 tttatcatca gatgttttta atacaggatt aatttcaaat aagctggcat ctgaacccac
    12121 gtaagctttg tacagttttt gtacaaaatc gaccatatct ttaaatgcag caccggataa
    12181 tcccaaatta aaggcaatcc ttcttgcctg aaaaggttga atgcccacag caggatctgc
    12241 ccattcttta aatatttttt caggatgatt ttctgctacc tcttctatat ccataccgcc
    12301 ttcggcagaa taaacaatcg cattgcattc tttggctctg tctaacaaaa cagacatata
    12361 aaactctttg gtaggcgaag caccttcata atatgcatct tcggctatga gaattttgtg
    12421 tactactttg ccttgcggtc ctgtttgagg cgttactaag gtcatgccta aaatagcttg
    12481 agccttttct ttgagtgcat caaaatcagg tgcaaatttc actccgccgc ctttgcctct
    12541 tcctcctgcg tgtatttgtg ctttaactac tacaccatct ctaccggtac gggctataat
    12601 ggttttgtag gcatcaattg cctgatctac ggtttcggcg actatacctt cctgtacttt
    12661 tattccaaat tgttttaaaa gttcttttcc ttgatattcg tgtaaattca taaactaaaa
    12721 ataagttgtg caaaggtaat taatttttta ggaaagcact ttgtgaaatg aagcactttt
    12781 tggattgaaa agcttggtta tccactttta tgtggagtaa taaaatagct tataatttca
    12841 aagcgatatt attccagatt tcttcagtta ttatgtcaac ttcctgcttg ccatttataa
    12901 taataatatt ttcattgcta tgtaatttct ccatggcctt gaaataattt tctcttactt
    12961 ttctaaggtt atcacttgtt tcata
//
LOCUS       DAOQII010000042        12948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2362_length_12948_cov_4.620181, whole genome shotgun sequence.
ACCESSION   DAOQII010000042 DAOQII010000000
VERSION     DAOQII010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2362_length_12948_cov_4.620181"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..381)
                     /locus_tag="PLF48_03365"
     CDS             complement(103..381)
                     /locus_tag="PLF48_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNF48395.1"
                     /translation="MTIYVANLHYGVKPEELQTVFENYGEVESVKIISDKFTGKSKGF
                     AFVEMPNAEQGAKAISELNSFELKGRNLVVNEARPKEERPKSNFNKRY"
     gene            complement(448..1647)
                     /locus_tag="PLF48_03370"
     CDS             complement(448..1647)
                     /locus_tag="PLF48_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HNF48396.1"
                     /translation="MTIQNYVYYNRLFKPLDLPLAYCDMDMLQQNINDIADRCSGTDK
                     KIRIASKSLRCRYLIQHILDNHPIYKGIMSFCGAETIFLLENNFDDILIGYPIINESE
                     IREISAWTKKGKKVYLMTDDKAHLEIINRIAAEENILQPVCMDIDMSTDFPGIHFGVL
                     RSPLNSICKAKKFIDQFENFPHIILKGVMGYEAQIAGLGDKNPANGIKNMLIPTLKKK
                     SIADFSKRREEIVKYIKSKKIELDFVNAGGTGSIESSKTEDWVTEITVGSGFYSPALF
                     DYYSNFRHQPAVGFALPIVRSPKVGYFTCLGGGYIASGTTGKDKQPLPYLPVGIELTE
                     NEGCGEVQTPFAYNGKEPIAIGDIILFRHAKAGEICERFNEFQLVSNGEIIKKVPTYR
                     GEGKSFL"
     gene            complement(1707..4154)
                     /locus_tag="PLF48_03375"
     CDS             complement(1707..4154)
                     /locus_tag="PLF48_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF48397.1"
                     /translation="MISFRKLITLLGFSIFFSTLFAKGTLTGIISDSTSGEKLFGVNI
                     ETNGIGTSSDIDGKYILELDEGSYLVTFSYLGYSSISQNIDIKDGQTQELNILLQAVA
                     TDLSDNEVVITGSMFEKRASEEVISIELIKPKALQNLNPVRLDEIARITPGLNVVDGQ
                     TNIRAGSGWTYGVGSRVMFILDGMPVLSPDRGDVKWTLLPVELAGQIEVLKGASSVLY
                     GSSAMNGTINILYPKPTSKPITRITVYQSFITAPKNKVYKWWKFPIPTFGVSFMRAHK
                     PKPNYDYIVGSNIYISNNHFERGQEFLARFNFKTRWINKKNENMSYGIGGSMMYNKEY
                     EFFYWQNKTDGAYKAGADNDFQNIRLTVDPFFQTYDKKNNKHELRGRLYFNRPSFNTI
                     TILQDTRYSFNKRYESKKFNIVAGAEHQYLWVKVPAFAGGGSKMANLLAGFTQLDKKY
                     NKLTLTAGVRFEMFKYEKNIGVTGSVWKNANGKTKFYLPGQWRCGLNYEISKNSFLRF
                     NIGQAYRFASFAEQFVNENVGSASQTHTVKLYDTIPPYIYVRDSSYTISHPLLHIHPN
                     TDLTPEYGFTSEIGFEQKWNVRKGKYTGNFDIAFFWQEYKNMIDINTIGPVTNADVNL
                     QFQNISKARIAGYEINWKNNIKIAPKHNMYMNVGYTYSMPVDLNQNAYVDVKNPFKYL
                     GFLFKNMFKTMKIDDAYATTLLKYRNRSLLTFDMEYNFNDQITIGADLRYYSSIENFD
                     VIFLNLNQINEYYVDNYYNTSRKGDAVLNVRAFYTYKKRHNFGLVIKNITNTEFWLRP
                     GKLEQPRSIGVQYRLEF"
     gene            complement(4272..6722)
                     /locus_tag="PLF48_03380"
     CDS             complement(4272..6722)
                     /locus_tag="PLF48_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF48398.1"
                     /translation="MKYLFSACLLFLIVNKIQAQEATISGIVKDIKTKEPLIGVNVSI
                     DSSTVGAVTDMDGKYELKSASGKVKVIFSYIGYDNIVENVNLKPNQKLTLDIDMGEAQ
                     SLLDEVVVTSSKFERKIGEETVSIDIIKPATLEKQNLNNVSDALQRSPGLTVVDNQVN
                     IRGGSGWSYGAGTRVLLLMDDMPILQADAGFPSFSSIPVENIGQIEIIKGAASALYGS
                     SAMNGIINFRTSYPTSEPHGKFSMFGTVYDNPNKNEVYGLLDAENRFTGVKVSEKVNK
                     NWWELDSVLLKGVSLGSGLITVRDTTIANPYQTRPHEFGFNFKYAEKLGKAKKLDFIL
                     NGMLYKKQSHRWGEFEKRGRITVNLRYRINEKISVGVNTNIIASQSQTFFLWGGLGIY
                     KYLPGAVTGIPTESRTFRATIDPFFRYVDSKGNSHKILTRYYRTSIDNTNNQDNASHY
                     IYGEYQYQRKIDKWNFVITTGAVGNYVLSNAQLFGDTKHHGYNVAGYLQLDKKFWKRL
                     NISYGFRFESNFIDGLKPETKPVSRIGINVECAKYTFVRASFGQGYRFPTIAELYVKT
                     ELSPTIAIGQNPSLKSEKGMSAELGIKQGIKLGRYFNAFVDVAAFYQQFKDMMEFNFV
                     PGAGKYIIEAKSLNVGSTEIIGAELTLMGEGKIAKKFPSTITFGYTFIQPKFKNYDPT
                     NKSDNGIADYNILKYRFRHLVNGTWDVSFGGFDFGTTIQYFSAMENYDYILGLLLPNT
                     FQFNYGKYKDNWESKKPAFRQKGDFILSLRTAYNFGKDKNFKLSFVVNNVTNREYTSR
                     TGLIEAPRNYGFRFDTTF"
     gene            complement(6810..7304)
                     /locus_tag="PLF48_03385"
     CDS             complement(6810..7304)
                     /locus_tag="PLF48_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48399.1"
                     /translation="MKRKFSLLAFVAVVAAFFAISCGTDTTETYDSAWVGTYAGSMEI
                     DTLAISNPAIDTLKHRPIIDTIIITDNGNASDNKLNMTTKVTGLSNEIVVSSATSGTL
                     TMTGKTIAISGVTVTNVSASGTLGLAGSNLHLHFTATNGTVFGIPGVLALVKPQLSGD
                     FVKQ"
     gene            7464..8882
                     /locus_tag="PLF48_03390"
     CDS             7464..8882
                     /locus_tag="PLF48_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNF48400.1"
                     /translation="MTASMISINPFDTSAIPQIVQHQRDFFNSGKTKDIEYRISQLKK
                     LRKIVVDNEQLIMDALKKDLHKSDMEAYATEVGFLLADIDHTLRDIRKLAKNRRAKTP
                     LFHQLGSSWVQPEPYGVVYVIAPWNYPFQLTLSPVVGAIAAGNTCVIKPSEMSEHTSV
                     LIEKLISENFEPGYLKVILGGIEESKALLEQKFDYIFFTGGTEIGRYIYQAAAKNLTP
                     VTLELGGKSPCFVDSTFNKKWGIQRIVWGKFTNAGQTCVAPDYILVDKNAKAAFVEAF
                     IETTKKFFGENPQQSPDFGRIINEKHFLRLARLLEKGNILMGGQTDIQNLYIAPTLID
                     GLSTDNPVMQEEIFGPILPVIEYNTIEEAIAFVNSRSKPLALYIFSDDSNYQNKILEN
                     TSSGNASINECLMHVGQFELPFGGVGDSGIGAYHGKYSFDIFSHLKGVLKKSTLSDFA
                     QRFPPYTKGGTTFIKKMIGWFM"
     gene            complement(8956..9300)
                     /gene="rplT"
                     /locus_tag="PLF48_03395"
     CDS             complement(8956..9300)
                     /gene="rplT"
                     /locus_tag="PLF48_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNF48401.1"
                     /translation="MPRSVNAVAAKARKKKVLKQTKGYFGRRKNVWTVAKNAFDKAQG
                     YAYVGRKNKKRDYRSLWIVRINAAVREFGMSYSVFIKKLADAKIDINRKVLADLAMND
                     PKAFEAIVNQVK"
     gene            complement(9371..9568)
                     /gene="rpmI"
                     /locus_tag="PLF48_03400"
     CDS             complement(9371..9568)
                     /gene="rpmI"
                     /locus_tag="PLF48_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNF48402.1"
                     /translation="MPKMKTNSSAKKRFKITGSGKIKRSQAYKRHILTKKSTKRKRAL
                     TGTTIIRETDMANAKKLLVLS"
     gene            9943..10134
                     /gene="rpsU"
                     /locus_tag="PLF48_03405"
     CDS             9943..10134
                     /gene="rpsU"
                     /locus_tag="PLF48_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNF48403.1"
                     /translation="MLIVDARENESLDRALKVFKKKFEKAGVVKQLRERQAFTLSSVK
                     RRTTVLKAIYRESIRQNED"
     gene            10201..11139
                     /locus_tag="PLF48_03410"
     CDS             10201..11139
                     /locus_tag="PLF48_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNF48404.1"
                     /translation="MLVAFLNYLTYEKRYSPKTVESYQTDLQQFFLFQQNEFQQADIT
                     QTHNRHIRTWVVALVNTNIKPASVKRKISALKSFFKYLQKTGKIQTNPAAKIVSPKIP
                     DRLPKFVAQKDLNTLLEDHNQDKNWTNQGQDSAQIKNYFSDDFFGRQENLIIDVLYSC
                     GLRRSELIHLKWTDVNLGTKQIKVTGKGNKQRLIPISSALVAALTNLKAEQENEYKDK
                     TKQDYVFLTKDGNQLYDKFVYRLVKEKLTLCTTVEKKSPHVLRHSFATHMSNNGAKLN
                     DIKELMGHASLASTQVYTHNTIEQLKEIYKIAHPKS"
     gene            11229..11531
                     /gene="raiA"
                     /locus_tag="PLF48_03415"
     CDS             11229..11531
                     /gene="raiA"
                     /locus_tag="PLF48_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNF48405.1"
                     /translation="MKIQIQSVHFDADVTLKDFINKKLNKLEQFYDRIIDSDVVLSME
                     QLNTQVKDKVVTIKTNVPGATIIAKEKSKKFEEAIDLAVDSLKKQLAKHKEKQNGH"
     gene            complement(11696..>12948)
                     /gene="aceA"
                     /locus_tag="PLF48_03420"
     CDS             complement(11696..>12948)
                     /gene="aceA"
                     /locus_tag="PLF48_03420"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNF48406.1"
                     /translation="KKDWAENPRWNGIKRPYTAEDVVSVSGSVEVEYTLAKKGADKLW
                     NYLQTRAWTAGLGALTGNQAVQEVQAGMPAIYLSGWQVAADANLGMEMYPDQSLYPAN
                     SVPAVVRRINNAFARRDQIQWLNQDGDIDWFAPIVADAEAGFGGNLNAFELMKGMIEA
                     GAAGVHYEDQLASAKKCGHLGGKVLVPTQEAINKLVAARLAADAMGVSTLLIARTDAE
                     AATLITSDIDERDRKFIVDGKRTSEGFYYVKNGIEAGIDRACSYAEYADLIWMETGNP
                     DLGLAKEFAQGVHARYPGKMLAYNCSPSFNWAKYMDVKAMETFREDLAAMGYKFQFIT
                     LAGFHALNSSMFSLAKDYMARGMAGFSEMQQREFAMEKDGFKAIKHQSFVGTGYFDAV
                     QNIAQQGLSSTTALAGSTETEQFH"
BASE COUNT         4131 a         2358 c         2116 g         4343 t
ORIGIN      
        1 gcgagataca ggagaataca atattcttgt tgtaaattgt tgtaaataaa aaggctgcac
       61 atggcagcct ttcttttttt tgacaaacaa aaatttcaat aattaatatc tcttattaaa
      121 gtttgacttt ggtctttctt ctttcggacg tgcttcattc actaccaaat ttcttccttt
      181 taattcaaaa gaatttaatt ctgaaattgc tttagcgcct tgttcagcat tgggcatttc
      241 tacaaaggca aaacctttgc tttttccggt gaatttgtct gagatgattt taacggattc
      301 aacctcaccg tagttttcga aaactgtttg cagttcttct ggttttacac cgtagtgtaa
      361 attcgcaaca taaatggtca tttaaaaaaa aatttaaggt ggcaaggtat agaatatatt
      421 ttacattacc taataaaagc tgatttttta taaaaatgat tttccttccc cccggtaagt
      481 aggtactttt ttgataattt ctccattgga aacgagctga aattcattaa accgttcgca
      541 aatttcaccg gctttggcat gtctgaacaa gataatatcg ccaatagcaa tgggttcttt
      601 gccattatat gcaaaaggcg tttgtacttc gccacagcct tcattttctg taagttcaat
      661 acccacaggc agataaggca gtggctgttt atccttccct gtagtaccgc tggcaatata
      721 tccgccaccc aaacaggtaa aatagcctac tttgggtgaa cgtactatgg gaagtgcgaa
      781 acccacagcc ggctgatgcc tgaaattgga ataataatcg aacagagcag gtgaataaaa
      841 gccggaacca acggtgattt ctgttaccca atcttcggtt ttgctgcttt caatactgcc
      901 ggtgccgcct gcattcacaa agtcgagttc tatctttttt gatttgatat actttacaat
      961 ttcttctctt cttttagaaa aatcggctat tgatttcttt tttaatgttg gaatcagcat
     1021 gttttttatg ccgtttgccg gatttttatc acccagacct gcaatttgag cttcatagcc
     1081 cattacacct ttaagtataa tgtgcggaaa gttttcaaac tggtctataa atttttttgc
     1141 tttacaaatg gaatttaatg gtgaacgcaa gacaccaaaa tgaatgcccg gaaaatcggt
     1201 ggacatgtcg atatccatac atacaggctg gaggatattt tcttcggcag caattcggtt
     1261 tattatttca agatgtgctt tatcgtctgt catcaaataa actttcttcc cttttttcgt
     1321 ccatgcagat atttcccgta tttctgattc gtttattatg ggatagccga ttaaaatatc
     1381 atcaaaattg ttttcgagca aaaaaattgt ttcagctccg caaaaactca taatgccctt
     1441 gtagatagga tgattatcta aaatatgttg aatgagataa cggcagcgca aggattttga
     1501 ggctattctt atctttttat ccgttcctga gcatctgtca gcaatatcat ttatgttttg
     1561 ctgaagcata tccatgtcgc aatatgccaa aggtaaatcc agtggtttaa atagccggtt
     1621 ataataaacg taattttgaa ttgtcatttc taaaattaaa aaaatccttt ctaaatgaaa
     1681 ggattttttg taatctcaac ttctaattag aattctaagc gatattgtac acctatactt
     1741 ctaggttgtt caagcttgcc agggcgaagc caaaattcgg tgttagttat attctttatg
     1801 actaatccaa aattatgacg tttcttgtag gtatagaaag cacgtacatt cagcactgca
     1861 tcacctttac ggctagtgtt gtaataatta tcaacatagt attcattaat ttgattcagg
     1921 tttaaaaaga taacatcaaa attctcaatg gaactgtaat atcttaaatc tgcgccgatt
     1981 gttatttggt cattaaaatt gtattccata tcaaacgtca ataagcttct atttctgtat
     2041 tttaatagtg tagtagcata agcgtcatca attttcatgg ttttaaacat gtttttaaat
     2101 aaaaatccta aatatttaaa tggatttttt acatccacat aagcattttg atttaaatct
     2161 accggcattg aataggtata gcctacattc atgtacatgt tgtgcttagg ggcaattttg
     2221 atattgtttt tccagttaat ttcatatccg gcaatacgag ctttagaaat attctgaaat
     2281 tgcaggttaa catctgcatt agtaactggt cctatcgtgt tgatgtcaat catattctta
     2341 tactcttgcc aaaagaatgc gatatcaaaa ttaccggtat attttccttt tcgtacattc
     2401 catttttgtt caaaaccaat ttcactggta aatccatatt caggagtcag atctgtattt
     2461 ggatgaatat gtaataatgg atgtgaaatt gtataagagc tgtctcttac atatatatat
     2521 ggaggaatag tatcatacag tttcaccgta tgtgtttgac ttgcacttcc aacattttca
     2581 tttacaaatt gttcagcaaa tgatgcaaat ctatacgctt gacctatatt aaaccttaaa
     2641 aatgaatttt tagaaatctc atagtttaga ccacatctcc attgaccggg taaataaaat
     2701 tttgtttttc catttgcatt tttccaaaca ctacctgtaa caccaatatt cttctcatac
     2761 ttaaacattt caaaacgaac tcctgcagtc agggtaagtt tattgtattt tttatctaac
     2821 tgtgtaaacc ctgcaagcaa atttgccatt ttagacccgc cacctgcaaa tgccggaact
     2881 tttacccata aatattgatg ttctgcacct gcaacaatat tgaatttttt actttcatac
     2941 cttttgttaa atgaatagcg agtatcttgt aaaatggtaa tggtattaaa tgaaggtcgg
     3001 ttaaaataga gtcttcctct taattcatgt ttattgttct ttttatcgta cgtctgaaaa
     3061 aatggatcta ccgttaaacg aatattttgg aagtcattat cggcacctgc tttataagct
     3121 ccatctgttt tattttgcca atagaaaaat tcatattctt tgttatacat catagagcca
     3181 ccaatgccat aagacatatt ttcatttttt ttatttatcc atctggtttt aaaattaaaa
     3241 cgtgctaaaa attcctgtcc tctttcaaaa tgattattag atatatatat attgcttcca
     3301 acaatgtaat cataattagg tttgggttta tgcgctctca taaaagaaac gccaaatgtg
     3361 ggtatcggaa acttccacca cttatatact ttgttctttg gtgcagtgat gaaggactga
     3421 tatacggtaa ttcgagtaat gggttttgaa gtaggtttcg ggtataaaat attaattgtg
     3481 ccattcattg ctgaggatcc ataaagtaca gaagatgcac cttttaagac ttcaatttga
     3541 cctgctaatt ccacaggaag taaagtccat ttcacatctc cacgatcggg agataaaaca
     3601 ggcataccat ctaatatgaa cattactctt gagcctacac cataggtcca accacttcct
     3661 gctctgatat ttgtttgacc atctactaca tttaagccgg gtgttatcct tgcaatttca
     3721 tctaacctca ccggatttag attttgcaat gccttaggct tgatgagttc aatagaaatt
     3781 acttcttcac tggcacgttt ttcaaacata gacccagtaa ttacaacctc attatcactt
     3841 aaatctgttg caacagcttg taataatata tttaactctt gtgtctgccc atctttgata
     3901 tcaatatttt gagaaatact ggaatatcct aagtacgaga atgtaacaag ataacttcct
     3961 tcatccagtt ccagaatata ttttccatca atatctgaag atgttcctat gccattagtt
     4021 tcaatattaa caccaaataa tttttcgcca cttgtagaat cggaaataat acctgtaagt
     4081 gttccttttg caaatagggt cgaaaaaaag atgctaaatc ccagtaaagt aattagtttt
     4141 ctaaatgaaa tcatggtctg tactttttac aaaaataagt tagatttagt ttaataatgc
     4201 agtaagtgaa ttaataaata gtaattaatt caatctattg ctaataagta accataaatg
     4261 gttatatcag cttaaaatgt agtatcaaaa cggaatccat aatttcgtgg tgcctctatc
     4321 aagcctgttc gagatgtata ttctctgttc gttacattat ttaccacaaa tgataatttg
     4381 aagtttttat ctttaccaaa attgtaggcg gttcttaaac tcaatataaa atcacctttc
     4441 tgacggaaag ctggtttttt gctttcccag ttatctttgt atttgccata attaaactgg
     4501 aaagtattgg gtaataataa gcctagtata taatcataat tttccattgc agagaaatac
     4561 tgaatagttg taccaaaatc aaaaccgcca aaagatacat cccaagtacc gtttactaaa
     4621 tgtctgaatc ggtatttcaa aatattataa tctgcgattc cgttatcact tttattagta
     4681 gggtcgtaat ttttgaattt aggttggata aaagtataac caaaagttat agtagagggg
     4741 aatttttttg caatctttcc ttcccccatc aatgttagtt cagcaccaat aatttctgta
     4801 ctacccacgt ttaatgattt ggcttcaata atatatttac cggctccggg aacaaagtta
     4861 aattccatca tatctttgaa ttgctgataa aaggctgcaa catcaacaaa ggcattaaag
     4921 taacgaccta attttattcc ctgtttaatt cctaattccg cactcatgcc tttttctgac
     4981 tttagcgacg gattttgacc aatagcaatt gttgggctta actccgtttt tacatataat
     5041 tcagctatgg taggaaatct ataaccttga ccaaatgaag ctctcacaaa ggtatatttg
     5101 gcacattcaa cgttgatacc aattctagaa acaggttttg tttccggttt taagccatca
     5161 ataaaatttg attcaaatct aaagccataa gaaatattca gcctcttcca gaatttttta
     5221 tctaactgta agtatcctgc tacattatag ccatgatgtt tggtgtctcc aaataattgt
     5281 gcatttgata aaacataatt acctacagct ccagttgtta tcacaaagtt ccatttatct
     5341 attttacgtt gatattggta ttctccataa atatagtgag aggcattatc ttgattattt
     5401 gtattgtcaa ttgatgtacg atagtatctg gtaagtattt tatgtgaatt tccttttgaa
     5461 tcgacatatc tgaaaaaagg atcaattgtc gctctgaaag ttctggattc tgtaggaata
     5521 ccggtaactg caccaggaag atatttgtaa atacctaaac ctccccataa aaagaaagtt
     5581 tgactttggc ttgcaataat attagtattg acacctacac ttattttttc atttattctg
     5641 tatcttaaat taacagtaat cctgccgcgt ttttcaaatt ctccccagcg gtgagattgt
     5701 tttttatata acattccatt taaaataaaa tccaattttt ttgctttacc taatttctca
     5761 gcatatttga aattaaatcc aaactcatga gggcgagttt gataaggatt agcaatagtc
     5821 gtatcacgta ccgtaattaa tccactaccc aaacttactc cctttaataa gacactatcc
     5881 agttcccacc aatttttatt tactttctca ctgactttta ctccggtaaa tcggttctct
     5941 gcatctaata atccatatac ttcattttta ttaggattat cataaacagt accaaacata
     6001 gaaaatttac catggggttc tgaagtagga tagcttgttc taaagttgat tatgccattc
     6061 atggcagaag aaccatatag tgcagaagcc gcacctttaa taatttctat ttgtccgatg
     6121 ttttctaccg gaattgaaga aaaagaggga aatccggcat cagcttgtag aataggcata
     6181 tcgtccatca ataaaagtac acgagtaccc gcaccataac tccaaccgct accacctcga
     6241 atatttacct gattgtcaac gactgttaag cccggactgc gttgcaaggc atcactaaca
     6301 ttatttaaat tctgtttctc aagtgttgcc ggtttgatga tatctatcga tacggtttct
     6361 tcaccgattt ttctctcaaa tttactgctg gttactacga cttcatccaa taaactttgc
     6421 gcctcgccca tgtctatgtc aagagtcaac ttttgattgg gttttaaatt aacattttct
     6481 acaatgttgt catatccaat gtaagaaaaa attactttta cttttcctga agcagatttt
     6541 aattcgtatt ttccatccat atctgtaacc gctccaacag tactactgtc aatgctgaca
     6601 tttactccaa ttaatggttc tttagtttta atgtctttaa caatcccgga aatggtagct
     6661 tcttgagctt ggattttgtt tactattagg aaaagtaaac aggcggaaaa tagatacttc
     6721 ataaataatt gatgtaaagt ttacaaaaat aataagataa ataaaaaaac cacccatttg
     6781 ggtggttttt tttagttggt atcctaaaat tattgtttta caaagtctcc gctcaattga
     6841 ggcttaacga gcgctaatac acccggtatt ccaaagaccg taccgttggt agcagtaaaa
     6901 tgtaaatgta aattactgcc tgcaagccct aatgttccac ttgctgacac atttgtaact
     6961 gtaaccccac taatagcgat tgttttacct gtcattgtaa gagtacctga agttgcgcta
     7021 gaaacaacaa tttcatttga taagcctgta actttagtag tcatatttaa tttgttatca
     7081 gaagcattac cattatcagt tattattata gtatctatta taggtcggtg ttttaatgta
     7141 tcaatagccg gattcgaaat ggctaaagta tcaatttcca ttgaccctgc ataagttcct
     7201 acccatgctg aatcataagt ctcagttgtg tcagtaccgc aactaattgc gaaaaatgct
     7261 gctactacgg caacaaatgc taataaacta aattttcttt tcatgtgatt ttattttaat
     7321 tattttgttg cacaaatata atgcctaatc tgaattacgc aagttttttt ttaaaaatta
     7381 ttgcaaatga ttaaccaatg tttagtagca aacaactcaa tttcatcaaa aaaggttata
     7441 ttgcagtata aaactaatta gttatgacag catccatgat ttcaattaat ccgtttgata
     7501 ccagtgccat accgcaaata gtgcagcacc agcgagattt ttttaattcg ggcaaaacaa
     7561 aagacatcga atatcgtatc agtcaattaa aaaaattaag aaaaattgta gttgataatg
     7621 aacagctcat tatggatgca ctcaaaaagg atttgcataa atcggatatg gaagcttatg
     7681 ccacagaagt tggttttctt cttgctgata ttgaccatac cttacgtgat attcgaaagt
     7741 tagcaaaaaa tagaagggct aaaacaccat tgtttcatca attgggcagt tcatgggtac
     7801 agcctgagcc ttatggtgta gtatacgtaa tagctccatg gaattatcca tttcagctta
     7861 cactttctcc ggtagtaggt gcaatagctg ccggaaatac ttgtgtgata aaaccttcag
     7921 aaatgtcaga acacacttct gtattaatcg aaaaactgat tagcgaaaat ttcgaacccg
     7981 gatatttaaa agttatactg ggcggtatcg aggaaagcaa agccttgctg gaacaaaaat
     8041 ttgactatat cttttttaca ggaggtactg agataggcag atatatttat caagctgctg
     8101 ctaaaaatct gacacccgtc actttggaat taggagggaa atcaccttgt tttgttgact
     8161 ccacatttaa caagaaatgg ggtatacagc gaattgtttg gggtaaattc actaatgccg
     8221 gacaaacctg cgtagctcct gattatattc tggtagataa aaatgcaaaa gcagcatttg
     8281 ttgaagcttt tattgaaacg acaaaaaaat tctttggaga aaatccacag caatcaccgg
     8341 attttggcag aattatcaat gaaaagcatt tcttacgatt agcaagatta ctggagaaag
     8401 gcaatatatt aatgggcgga caaactgata ttcaaaatct ttatattgct cccacgctga
     8461 tagatggtct ttctactgat aatccggtga tgcaggaaga aatttttgga ccgatacttc
     8521 cggtaataga atataacacc attgaagaag ctatagcttt tgtcaattca agaagcaaac
     8581 cactggcgtt atacattttt tcagacgata gtaactatca aaataaaatt ttggaaaata
     8641 cgtcatccgg aaatgcatcc ataaacgaat gcctgatgca tgttggtcag tttgaattgc
     8701 cctttggagg agtaggagat agcggtattg gcgcatatca tggtaaatac agttttgata
     8761 tcttcagtca cttgaaaggc gtattgaaaa aatccacact ttcagatttt gcacaacgct
     8821 ttccacctta cacgaaaggt ggtactacct tcattaaaaa gatgataggt tggtttatgt
     8881 agagtaatta aatgtggtta gatataaaaa aaggctgtca ttgctgacag cctctttttt
     8941 ataaaagatt ttgttttatt ttacctgatt tacaatagcc tcgaatgctt ttggatcatt
     9001 catggctaaa tcggccaata ctttgcgatt aatgtcgatt ttcgcatcgg ctaatttttt
     9061 gatgaataca gaataagaca tgccaaactc acgaactgcc gcattgatac gtacaatcca
     9121 taagcttctg taatctcttt tcttgttttt tctgcctaca taagcatatc cttgtgcttt
     9181 atcgaaggca tttttggcta ctgtccatac attttttcta cgaccgaaat aacctttggt
     9241 ctgcttgagg actttctttt tgcgggcttt tgccgctact gcgttgactg aacgtggcat
     9301 actttataat tttaatagtg ataaataaaa acccatcggc gctgccttgc cttatggtat
     9361 aatagtttta ttagcttaaa accagcaatt ttttagcatt tgccatatcc gtttcacgga
     9421 taatagtagt gccggttaaa gcgcgttttc ttttagtaga tttttttgtt aagatatggc
     9481 gtttgtaagc ctgtgagcgt tttatttttc cgcttccagt aattttaaag cgttttttcg
     9541 cgctcgaatt ggttttcatc tttggcatat actcgatttt aaactgttta aattgataat
     9601 cttgccttaa ttgggcaaaa tgaggtgcga aaatacaaaa aaaatgagaa tttaattaaa
     9661 aacagaaaac agaaatgtgt gtttaatgtt aaatttaatt catatcatcc ttttaatcag
     9721 tagaaagaaa tgtccaccta ttttgaaatc aatataaata gaatttttta aaaagaagtt
     9781 tatatccaat tcgaaataaa cgatgtaaaa atttaaataa aatgagattg ttataattga
     9841 ctaagctcgc ttataacttc tcacaattta ttaatattgg aatattggct gaaaaatgtt
     9901 atctttgcac cctattctat aaaataaaag aggtattaaa ttatgttaat cgttgatgca
     9961 agagaaaacg agtctttaga cagagcactt aaggtgttta aaaagaaatt tgaaaaagca
    10021 ggagttgtta agcaacttcg tgaacgtcaa gcatttacac tttcatctgt aaaaagacgt
    10081 actaccgtac ttaaagcaat ctaccgcgag tctatcagac aaaacgaaga ttaatagttg
    10141 caactttcat tttttttatt tatcttgtaa gttcgattgt gaatttaaca ggttaagaaa
    10201 atgttagtag cgttcctgaa ttaccttacc tacgaaaaga gatattcacc taaaactgtt
    10261 gaatcgtatc aaaccgattt gcaacagttt tttttatttc agcaaaatga atttcagcag
    10321 gctgatatta cgcagaccca taacaggcat attcgtacct gggtggttgc cttggtcaat
    10381 acgaatatta agcctgcatc tgtaaaaaga aaaatatccg cattaaaatc attttttaag
    10441 tatttacaaa aaacaggaaa aattcaaact aatcctgcag ctaaaatagt ttctcctaaa
    10501 ataccggacc gattgccaaa attcgtagct caaaaagatt tgaacacatt attggaagat
    10561 cataatcagg ataaaaattg gacaaaccaa gggcaggata gtgcccaaat caaaaattat
    10621 ttctcagatg attttttcgg acgacaagaa aatttgatta tcgatgtatt atattcttgc
    10681 ggtttgcgta gaagtgaatt gattcactta aaatggacgg atgtaaattt aggtactaag
    10741 caaataaaag ttaccggtaa aggaaacaaa caacgcctga ttccgattag ttctgcatta
    10801 gttgctgcac ttacaaacct caaagctgaa caagaaaacg aatacaagga taaaacgaaa
    10861 caggattatg tttttttaac caaagatgga aaccaacttt atgataaatt cgtttatcgt
    10921 ttagtaaaag agaaattaac tttgtgtact acagtcgaaa aaaaatcacc acatgttttg
    10981 cgccattcat ttgccacgca tatgagtaat aatggtgcta aattaaatga tattaaagaa
    11041 ttgatgggac acgccagttt agcttcgacc caagtgtata cccataatac cattgaacaa
    11101 cttaaagaaa tatataaaat tgctcaccca aaatcatagc ctatgtgtaa tttttaaaac
    11161 actactgcaa cgaatagaaa gattaaaaaa tattattaca tccattcaaa attaaattta
    11221 ttatgtctat gaaaattcaa atccaatccg tgcactttga tgcagatgtt accttgaaag
    11281 attttatcaa caaaaaatta aacaaacttg agcagtttta tgatcgcatt atcgactcag
    11341 atgtggtctt gtcgatggaa caactcaata cacaggtaaa agataaagta gtgaccataa
    11401 aaacaaacgt gccgggagca acaattatag ctaaagaaaa gagtaagaaa tttgaagaag
    11461 ccattgattt ggcagtagat tcactcaaaa agcaattagc caaacataaa gaaaaacaaa
    11521 acggacatta aaatgttgtt cgctaatata accaaatggc gcgccgaagg cgcgccattc
    11581 gtttttaatt tgactaatat tttatttcaa ctaaaaaagc cggaaatgat ttccggcttt
    11641 tttctgaatt gtatttttta tcatgttgaa actagcgtca cataataatt ttttatcaat
    11701 gaaattgttc tgtttctgta cttcccgcta atgcagttgt tgacgaaagt ccttgttgag
    11761 ctatattttg tacagcatca aaatatccgg ttcctacaaa cgattggtgt ttgatggctt
    11821 taaatccgtc tttttccata gcaaactctc tttgttgcat ttcgctgaag cctgccattc
    11881 cgcgtgccat ataatctttt gccaaactga acatagaaga gttcaaggca tggaaacctg
    11941 ccaatgtaat aaactggaat ttataaccca ttgccgctaa atcttcacgg aatgtttcca
    12001 ttgcttttac atccatatat tttgcccagt tgaaagatgg tgaacagttg tatgccaaca
    12061 tttttcccgg atatctcgca tgaacgcctt gagcaaattc tttagccaag cccaaatcag
    12121 gattacctgt ttccatccaa attaagtccg catattccgc ataagaacaa gcacggtcaa
    12181 tacctgcttc aataccattt tttacatagt aaaatccttc tgatgtgcgt ttgccgtcaa
    12241 caataaattt acggtctctt tcgtcaatat cagaagtaat caatgtagct gcttctgcat
    12301 ctgtacgggc aattaaaagg gtagaaacac ccatagcatc tgctgccaaa cgcgcagcta
    12361 ccaatttatt gatggcttct tgagtaggta ctaacacttt tccgcctaag tgaccacatt
    12421 tttttgcaga agctaactgg tcttcatagt gtacacctgc tgcaccagct tcaatcatgc
    12481 ctttcattaa ttcaaatgca tttaagttgc cgccaaaacc tgcttcggca tcagccacga
    12541 taggagcaaa ccaatcgata tctccatctt ggttcaacca ttgaatttgg tctctgcgag
    12601 caaacgcatt attaattctg cgtaccacag caggaacaga gttggcagga tataatgatt
    12661 gatccggata catttccata cctaagtttg catctgcagc tacttgccag ccggataaat
    12721 aaatagcagg cataccagct tgcacttctt gtacagcctg gtttcctgtt aatgcaccta
    12781 atccagccgt ccatgctctt gtttgaagat aattccaaag cttatcggca ccttttttag
    12841 ccaaagtgta ttccacttct accgaacccg aaacgcttac tacgtcttct gctgtataag
    12901 gtcttttaat gccattccaa cgtgggtttt cagcccagtc ttttttta
//
LOCUS       DAOQII010000043        12947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2363_length_12947_cov_5.181663, whole genome shotgun sequence.
ACCESSION   DAOQII010000043 DAOQII010000000
VERSION     DAOQII010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2363_length_12947_cov_5.181663"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /gene="aspS"
                     /locus_tag="PLF48_03425"
     CDS             complement(<1..1482)
                     /gene="aspS"
                     /locus_tag="PLF48_03425"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNF48407.1"
                     /translation="MYRSHTCGELRISDINKEVQLSGWVQTTRNFGELLFIDLRDRYG
                     ITQLVFNNETNNLLFDRAKRMGREFVLQAEGKVVERSNKNPKNPTGDIEILVHDVRIL
                     SISKVPPFTIENETDGGDDLRMQYRYLDLRRSEVQEKIMFRHKVGHEVRTYLNANGFL
                     DIETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMVSGFDKYYQITRCF
                     RDEDLRADRQPEFTQIDCEMAFVDQEDILNMFEGLIKHVFKTVKGIELSDFPRMQYDD
                     AMRLYGSDKPDLRFGMSFVEMSDIVKGKGFGVFDNAELVIGINAENCAEFTRKQIDEL
                     TDFVKRPQIGATGLIYLRWNNDGTLKSSVDKFYNEEELKKWAEAANMKQGDLLLVLCG
                     TTDKVRKQMNELRLEIGNRLGLRKKDEFKPLWVLDFPLLEFIEEDNRWFAMHHPFTSP
                     KKEDIEFLYTDPARVRADAYDLAINGVEVGGGSIRIFDKELQAK"
     assembly_gap    1688..1787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2108..3457
                     /locus_tag="PLF48_03430"
     CDS             2108..3457
                     /locus_tag="PLF48_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48408.1"
                     /translation="MKKVLSVITILVVTSAIKAQPFAGAHYLSKDAILTQIINPAFPA
                     ASDMKWQANFFGFDASLGNNHFSLVGKPKDWIKHWDKNAYVQENLDGKPKNLFIGFNV
                     MGPSFMYRIKKNTISFGTRVRAITTFNDLDENFITSLYNDFSHIQNWNSSFNDTRLTG
                     SVNVFHEFNLGYARSFKLKERHYLHAGASVKMTTNIFNAQLSVNHLDFNRYYNIGMSD
                     TMINAGNTEFELLVSNAVTDNDFNYHFGINGFGIDAGAIYEFRKKNSEEHILMVGFSV
                     NDIGFNEYKTGKYSRSFVGNNQDVPLSHLVESDNYTNINIDAILDSLGDYTIPSKKQK
                     IYLPTTINVFLDARIVSKFYVNANFQFNPLSFKHGLGKANMPNDITITPRFETRIFSV
                     FTPLNWNKYEGINFGAGVKIAQFTLGSKNIITSFAKKRFTGINFYMNVGFGKVKKKE"
     gene            complement(3583..4125)
                     /locus_tag="PLF48_03435"
     CDS             complement(3583..4125)
                     /locus_tag="PLF48_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48409.1"
                     /translation="MFAILSCKSKNNLQSSLGDFPSSKKEVPRKNPINLDSIYDNFRD
                     SIISSCNQNSNFKLNLSNDTELIKLEAIYLGSIITKSGNGIKFLNTIQYYQLKKYPLH
                     ASSGTFLFNKQNKRIGNYWYSSDWELPKRIAENSLVFSYNNSYCKYDTSVSFYDSIPS
                     FLFVPCSKFGGNIYDFYRNE"
     gene            complement(4187..4693)
                     /locus_tag="PLF48_03440"
     CDS             complement(4187..4693)
                     /locus_tag="PLF48_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48410.1"
                     /translation="MSKLMYIILITLFTNCSSNISKNDKYDLSDRANLLALNENVIGL
                     EYIIKKALKDEIEEYHVKYLGKIKKSNGSCLKFITNVLYCGKNTDSPHANAALYVYND
                     KNIELGYYSLGPIWSLPSKIVANNLVFDYSDSYCNQKTIINFQDSIPSQIYIKCTEKG
                     GDIYVFNK"
     gene            complement(4856..5155)
                     /locus_tag="PLF48_03445"
     CDS             complement(4856..5155)
                     /locus_tag="PLF48_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48411.1"
                     /translation="MKIYFSDTLIVSNEKIDVRKVEDKEIEVWISNDSIQKKISNLIK
                     PTIILEFNGNKYIAKPNLIYSSSIPNECDYFYPINKEGKVFNINNGIILFYKILN"
     gene            complement(5202..6218)
                     /locus_tag="PLF48_03450"
     CDS             complement(5202..6218)
                     /locus_tag="PLF48_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48412.1"
                     /translation="MKNLLFLFVCCVSLNTVAQLNPYAQFGYEPKNEYVINKSDKLEI
                     ANTDTSSTRQLLLFDFANKKLFVLNRADSIITIYDIPKDVLAKWISVDPKASKAPDWS
                     PYRSFFDNPIRFIDPDGQWEADSKGNLKAEKGDNAQTLAKFQGIKYGEALKQLKSQGY
                     TVNSKGILNLKVGDQLMLKNNYTESIKNSTGQFSTDVMMRLAPAPAGSGPGIDRGTPE
                     DKYNCWGSAIAGSQGNKIDNSVGIPLGSTFDAKLSSDYTPTTQANAQFGKTVLRFADA
                     SGVQHGAVYYGKNNAGEIYVYSKNGWHAKPEVMKLSDLMLKIPDYGTVQGINTGESGY
                     YQPK"
     gene            complement(6215..7207)
                     /locus_tag="PLF48_03455"
     CDS             complement(6215..7207)
                     /locus_tag="PLF48_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48413.1"
                     /translation="MFFLLFVASLSSFSQSMSATKKTELYKSAYTEICDMLSGKKTLN
                     LKRAIFITENAYVGGTMNYMNYDNQIKKIAFSLKQMIKDKHIEGYKTAGNWAAFVYMT
                     DSITYNNNHPYSYDFVDFFGDSSWTSQFVTKLLNTHVGNCHSLPLLYKVLCDEIGAKA
                     YIAFAPSHLYIKHQDENGKWVNLEMTNGSFSRDEWIMEQSAITIEQIKSGIYMNATTE
                     KELIAFMLFDLQSCYQRSFSCDSFQLKMIETGLAYFPNCINLQLAKAFHYQQVMLKEK
                     KQAYPNTQVIKDCIAKLKQQDKIFEELGYKDESPEHYEAWIKSVEEEKLKRNIK"
     gene            7380..8681
                     /gene="ffh"
                     /locus_tag="PLF48_03460"
     CDS             7380..8681
                     /gene="ffh"
                     /locus_tag="PLF48_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNF48414.1"
                     /translation="MFENLQDKLEGAFKNLKGEGKLTELNIASTIKDIRRALVDADVN
                     YKIAKEFTDKIKDEAMGKNVLTSVSPGQLMVKIVSDELTTLMGGTVTEINVKANPTII
                     LISGLQGSGKTTFSGKLANFVKTKLNKTPLLVACDVYRPAAINQLTVLGEQIGVEVYS
                     EIENKNPVQIAQNAIAHAKQKGQNVIIVDTAGRLAVDEEMMNEIYNVKQAIQPQETLF
                     VVDSMTGQDAVNTAKAFNDKINFDGVVLTKLDGDTRGGAALSIKYTVDKPIKFVSRGE
                     KLDTLDVFYPERMAQRILGMGDIVSFVEKAQEQFDEKEAQRLSKKIAKNQFDFDDFLQ
                     QINQIRKMGNIKDLLGMIPGVGKAIKDIDIDDNAFKKIEAIIFSMTPEERKNPDILNG
                     KRRERLAKGSGNSIQDVNNFIKQFDQMKSMMKKFQGMGMKK"
     gene            8898..9644
                     /locus_tag="PLF48_03465"
     CDS             8898..9644
                     /locus_tag="PLF48_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNF48415.1"
                     /translation="MKQFGIIGFPLSHSFSPGYFAAKFECEDITDANYAVFPLEKIEY
                     FNDLCLEFPNLKGLNVTIPYKEKIIAYLDELSEEAQKIEAVNTILFSDGKKTGYNTDI
                     YGFENSLQPLLNNTHKQALILGTGGAAKAVEYVLQKLGIGYRHVSRHETENAYCYEDL
                     FDNDSILQAHTLIINTSPIGMYPNTNECPNIPYEKLTQAHLLYDLVYNPENTLFMQCG
                     AAQGATVKNGLDMLYLQAEKSWEIWNKKPV"
     gene            complement(10293..10712)
                     /locus_tag="PLF48_03470"
     CDS             complement(10293..10712)
                     /locus_tag="PLF48_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48416.1"
                     /translation="MEVADYVKDDGISGVAILAHEIYEGFQHQGKGISNYETAHDIAL
                     RLQGMIDGYTNVKNIAQDKILFGTTEFTYTTNNGKNMKTTVNIKDNNFLKTTTTIDVT
                     PKIIIPEPSKYQDCSVCAEYERKQLELAKQLKKYFNK"
     gene            complement(11253..>12947)
                     /locus_tag="PLF48_03475"
     CDS             complement(11253..>12947)
                     /locus_tag="PLF48_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNF48417.1"
                     /translation="ERQRVIDFLNSIPCNAYVLGYSFFNAGYSQWDADSTSPLDQTLF
                     TAFERIGVNTIRNQRENEPFVFFIHNCDPGFNKIQISRAPTANNPAAIIDTSFTFSGT
                     WNKGTMQSTIIGPAKNWKTFQMDWHALENPYYDYSNVSIRGFDTSGTSTIYMDNLLKG
                     IPIDISSIDAKKTPFIQLTWNTQDDSFGTAPQMDFWRVTYDKVPEAVVNPTIHFSKTR
                     DTIVSGETLSLAVAVENVTPINMDSLLVRFTIRDASGNIIVVDQRYAPLPGKQYQIIN
                     FNYDFAGFNFTGLNTITVEVNPDTDQPEQFHFNNFAEFTVYVLRDRINPILDVTFDGR
                     HITDGEYVSSTPEILLRLKDENKYLALNDTNEFLLYLYYPNAPTTPVLVDLQQSDITF
                     IPADPSNLSRNNEARLIYKPTLQDGTYELRAQGIDRSRNDAGKYEYRIKFKVDSKPTI
                     THVLNYPNPFSTSTQFVFTITGQQVPDKLMIQIFSASGKVVREISKEELGNLHIGTNV
                     TDFKWDGTDMYGDRLANGVYFYRVYTKNTDGTNTEIKETGIDKFFKHGYGKLYIMR"
BASE COUNT         4042 a         2293 c         2188 g         4324 t
ORIGIN      
        1 tttagcttgt agctctttat caaaaatacg gatagagcca ccgccgactt ccacgccatt
       61 aatcgctaag tcataagcat ctgctcgtac tctagcaggg tctgtatata aaaattcgat
      121 gtcttctttc ttcggtgaag taaacggatg gtgcatcgca aaccaacgat tatcttcttc
      181 aataaactct aacaaaggaa aatctaaaac ccacaaaggt ttaaattcat cttttttgcg
      241 caagcccaaa cggttgccta tttccaaacg caattcgttc atttgtttac gcactttatc
      301 cgtagtgcca cataaaacta agagtaaatc gccttgtttc atgttggcag cttccgccca
      361 tttttttagt tcttcttcat tgtaaaattt atctactgaa gattttaaag tgccgtcatt
      421 attccatctt aaataaatta aacccgttgc accaatttgc ggacgtttta caaaatccgt
      481 gagttcgtct atttgtttac gtgtaaactc agcacaattt tccgcattaa taccaatcac
      541 taattctgca ttatcaaaca caccaaaccc ttttcctttt acaatatcac tcatctcgac
      601 aaacgacata ccgaaacgca aatcgggctt gtcgctgcca tacaaacgca tggcatcatc
      661 atattgcata cgaggaaaat cactcagctc aattcctttc accgttttga aaacatgttt
      721 gattaagcct tcaaacatat tcaatatatc ctcttgatct acaaacgcca tttcgcagtc
      781 tatttgcgta aactcaggtt ggcggtcagc ccgcaaatct tcatctctga aacatcgggt
      841 tatctgataa tatttatcaa aacccgacac catcagcaat tgcttaaagg tttgcggtga
      901 ttgaggaagc gcataaaatt gtcccggatt catgcggctc ggtaccacaa aatcgcgtgc
      961 accttccggc gttgacttaa ttaagaatgg tgtttcaata tctaagaaac catttgcatt
     1021 caaataagtg cgcacttcgt gacctacttt atgacgaaac atgatttttt cctgcacttc
     1081 gcttctgcgc aaatccagat aacggtattg catacgcaaa tcgtcaccac catccgtttc
     1141 attttcaata gtaaacggag gtactttaga tatggaaaga atgcgcacat cgtgcactaa
     1201 aatttcgata tcgcctgttg ggttttttgg gtttttgtta gagcgttcta ccacctttcc
     1261 ttcggcttgc agcacaaatt ctcgtcccat gcgttttgct ctgtcgaaca gtagattatt
     1321 cgtctcatta ttgaatacta attgcgtaat gccgtatctg tcgcgcaaat caataaataa
     1381 aagttcgccg aagttgcgcg tggtttgcac ccagcccgaa agctgaactt ctttgttaat
     1441 atcgctgatg cgtaattcgc cacaggtatg acttctgtac atgataaaag tattaagtag
     1501 tgagtaataa gtagtaagaa aatcttacaa cttgggtgca aaaataagaa gaaaatcagc
     1561 aattagctaa ttagcaattc gataattcga taatttgcta attcgttaat gaataagcca
     1621 tgtagtggca ttgcttgcac aaatagtctt aatttcctta atgccttaat ggtaaatatt
     1681 aattagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac ttagaggtat
     1801 tattgcacaa aaagtcttaa tttccttaat gccttaatgg tatagaattc tataatctga
     1861 aaataaataa gccaagtagt ggcattgtac gtaagcagtc ttaattttct taaagcctta
     1921 ttgataaata ttaattagcc acttagaggt attattgcac aaaaagtctt aatttcctta
     1981 atgccttaat ggtaaataat ccgataaaac aagttcaagt aaaaaccgac aaattataaa
     2041 gaactgtcta taagttgtct attagttttt tgaatgtgca ttatattttt gcagtaaatt
     2101 ataagctatg aaaaaggtct tatcagttat tactatttta gtcgtaacgt cagctataaa
     2161 agcacagcca tttgccggag cgcattattt gtcgaaagat gccatactta cacaaattat
     2221 caaccctgct ttccctgcag cctctgacat gaaatggcaa gccaacttct tcggttttga
     2281 tgcctctttg ggcaataacc atttttcttt agtaggcaag cccaaagact ggattaaaca
     2341 ctgggataaa aatgcttatg tacaggaaaa cttagacggc aaacccaaaa atctctttat
     2401 cggctttaat gtgatggggc catcgtttat gtaccgcatc aaaaaaaaca ccatcagctt
     2461 tggcacacgt gtgcgagcta tcacgacatt caacgatttg gatgaaaatt ttatcacctc
     2521 actttataac gattttagtc atatccaaaa ctggaatagt agttttaacg atacgcgcct
     2581 tacgggttct gtcaacgtgt ttcatgaatt taatttaggc tacgcccgaa gtttcaaact
     2641 caaagaacgg cattatctgc atgcaggggc atccgtaaaa atgacaacga acatttttaa
     2701 tgcacaactt tctgtcaacc acctcgactt caaccgctac tacaatatag gcatgagtga
     2761 tacgatgatt aatgccggta atacggagtt tgaattatta gtcagcaatg cagttaccga
     2821 caacgatttc aactatcatt ttggcatcaa tggttttgga atagatgcag gtgcaattta
     2881 tgaattcaga aaaaagaatt ccgaagagca tattttgatg gtcggctttt cggtgaatga
     2941 tattggattt aatgaataca aaaccgggaa gtatagtcgc agtttcgtgg gcaataatca
     3001 ggatgtgcca ttgtcgcatt tagtagaaag cgataattat acgaatatca atatcgatgc
     3061 gattttggac tctttaggtg attatacgat tccgagcaaa aagcaaaaaa tctatttacc
     3121 cactaccatc aatgtttttt tagacgcacg cattgtatct aaattttatg tcaatgcgaa
     3181 tttccagttc aaccccttgt ctttcaaaca cggacttggc aaagccaaca tgccgaatga
     3241 tatcactata acaccgcgtt ttgaaacccg tattttcagt gtatttacgc cattaaactg
     3301 gaataaatat gagggcatca attttggtgc aggagttaaa atagctcagt ttactttagg
     3361 ttcaaaaaat atcattactt cctttgcaaa aaaacgcttt acaggaatca acttttatat
     3421 gaatgtaggc tttggcaaag tgaagaagaa ggagtaaggg aaagtagtaa gtaggaaggt
     3481 gatggtggtt ttaaaaaaat taatatagtt aagagtattg gaattatagt tgccagattt
     3541 tggcattgat tttataaaac gaaatatatt aaagtgtgat ggctattcat tcctatagaa
     3601 atcataaata tttcctccaa atttagaaca aggaacaaat aaaaaagaag gaatactgtc
     3661 atagaaacta acactcgtat catacttgca ataagaatta ttgtatgaaa agactaatga
     3721 attttcagct attctttttg gcagttccca atcagaggaa taccaatagt taccaattct
     3781 tttattttgc ttattgaata aaaaagttcc ggatgaagca tgtagcggat atttttttag
     3841 ttggtaatat tgtatggtat tcaaaaattt aattccatta ccggatttag taattattga
     3901 gcccaaataa attgcttcaa gttttattaa ctctgtgtcg tttgaaagat taagtttgaa
     3961 attagaattc tgattacacg aactgattat agaatctcta aagttgtcat aaattgaatc
     4021 gagattaatc gggttttttc ttggaacttc ttttttagaa gatggaaaat ctcctagact
     4081 gctttgtaaa ttgttttttg atttgcaact taatatggca aacatgatat atgtgaatga
     4141 ttttagtaaa aaaaatacac ggcataaatg tgtgttttta atatttttat ttattaaaca
     4201 catatatatc tccgcctttt tctgtacact ttatgtatat ttgactaggg atactatctt
     4261 gaaaattaat aatagtcttt tgattgcaat atgaatcaga ataatcaaaa acaagattat
     4321 ttgcaactat tttactaggt aaagaccaaa ttggtccaag gctataatat ccaagttcaa
     4381 tatttttatc attatataca tataatgctg catttgcatg aggagagtca gtattcttcc
     4441 cacaataaag cacattagtg ataaacttta aacatgagcc atttgatttt tttatcttgc
     4501 ctaagtattt aacatgatat tcttctattt catcttttaa tgccttttta attatgtatt
     4561 ctaatccaat tacattttca ttaagagcta ataaatttgc tctatcactt aaatcatact
     4621 tatcattctt tgagatgtta gaactgcaat ttgtgaataa ggtaataaga ataatataca
     4681 ttaatttact catggttcag tagtatagtt tgctgtggat tgtggcagtg ttaatcttgc
     4741 tttttcaatc aaaatccatt cagagctggg atgagaactc ctatctgttt cttgaaaaaa
     4801 tgttttttgt gttacctgaa attgcatttt atatataata attatactat tatgtttaat
     4861 ttaatatctt ataaaaaagt attatgccat tattgatatt aaaaactttg ccctctttgt
     4921 ttattggata aaaataatca cattcattag gaatagatga actgtaaatt aaattcggtt
     4981 ttgcaatgta tttgttgcca ttaaattcta atataatggt aggcttaata agattactaa
     5041 tttttttttg aatactgtca tttgatatcc aaacttcaat ttctttgtct tctactttac
     5101 gaacatctat tttttcatta gaaactatta aagtatctga aaaataaatt ttcatatatt
     5161 taattgattt ttgttcttgc ccataacatg aactcaataa gctacttagg ctgataatag
     5221 ccactttcgc cagtgttaat accttgaact gtaccatagt ctggaatttt taacattaaa
     5281 tctgatagtt tcattacttc gggttttgca tgccacccat tttttgaata aacatatatt
     5341 tctcctgcgt tatttttgcc ataatataca gcaccgtgct gtactcctga cgcatcagca
     5401 aatcttaaaa cagttttccc gaattgtgca tttgcttgtg ttgttggtgt ataatctgaa
     5461 gaaagcttcg catcaaaggt actacccaaa gggataccta ctgaattatc aattttattt
     5521 ccttggcttc cagcaatcgc tgatccccaa caattgtatt tatcttctgg agttcctcta
     5581 tcaataccag gaccacttcc agctggtgct ggtgctaatc tcatcataac atcagtacta
     5641 aattgacctg ttgaattttt tatagattct gtataattat ttttaagcat taattgatca
     5701 ccgactttca gattcaagat tcctttacta tttacagtat aaccttggct tttaagttgt
     5761 ttcaatgcct caccatattt tatcccttga aatttagcaa gagtttgagc attatctcct
     5821 ttttctgcct ttaaattacc ttttgaatct gcttcccact gaccatcagg gtctataaat
     5881 ctaatcggat tatcaaaaaa tgacctgtaa ggagaccaat ctggtgcttt tgatgctttg
     5941 gggtcaacac ttatccattt tgccaacaca tcttttggta tgtcatatat tgtaataata
     6001 gaatctgctc tatttaacac gaatagtttt ttattggcaa aatcaaataa tagcaattgc
     6061 cttgtgcttg aggtatctgt attggcaatt tcaagcttat cagacttatt tatcacatat
     6121 tcattcttgg gttcataccc aaattgcgca tatggattta gttgcgccac agtatttaag
     6181 ctgacacaac agacgaataa aaataataga tttttcattt tatatttctt tttagttttt
     6241 cctcttcaac agattttatc caagcctcat agtgctcggg gctttcatct ttataaccca
     6301 actcctcaaa tattttatcc tgttgtttaa gcttagcaat acagtctttt atgacttgcg
     6361 tgtttgggta tgcttgcttt ttttctttta gcataacctg ctgataatgg aatgcctttg
     6421 ctaattgaag atttatacaa ttcggaaaat aagccaaacc tgtttctatc atttttaact
     6481 gaaatgaatc acatgagaaa cttcgttgat aacaactttg caaatcaaaa agcataaaag
     6541 cgattaattc tttttctgtc gtggcattca tatagatacc gcttttgatt tgctcaatag
     6601 taatggcaga ttgctccatt atccattcgt ctctggaaaa actaccatta gtcatttcta
     6661 aatttaccca tttcccgttt tcatcttgat gcttaatata taaatgactt ggagcaaaag
     6721 caatataagc ctttgcccca atttcatcac aaagcacttt gtataacagc ggtaatgaat
     6781 ggcagttgcc tacatgtgta tttagtagtt ttgttacaaa ttgagaagtc caactcgaat
     6841 caccaaaaaa atcaacaaaa tcatatgaat aaggatgatt gttattgtaa gttattgaat
     6901 cagtcatata aacaaatgca gcccaatttc cggcagtttt atatccctct atatgtttgt
     6961 ctttaatcat ttgtttgagc gaaaatgcaa tttttttaat ttggttatca taattcatat
     7021 agttcatagt gccacccaca tacgcatttt cagtgataaa aattgctcgc ttcaaattca
     7081 atgttttctt accggacagc atatcacaaa tttcagtgta agcactctta tacaattcgg
     7141 tctttttagt agccgacata ctttgtgaaa aagaagacag tgatgctaca aatagcaaaa
     7201 agaacaaaaa cagatatttt aacttttgaa tcattactga attaaagata agtaattatt
     7261 ttcattaaaa aactactatt atggattttt tttactattt tttgatggca gagtgtcgtt
     7321 aaacccataa tttcatcggc tttttttatt acttttgcac ctctttaaaa gttaaatgta
     7381 tgtttgaaaa tctgcaggac aagctcgaag gtgcctttaa aaatctgaaa ggtgaaggca
     7441 aactcaccga actcaatatt gcttctacta tcaaagatat acgccgcgcg ttggtagatg
     7501 ccgacgtgaa ctataaaata gcgaaagaat ttaccgacaa aatcaaagac gaggcgatgg
     7561 ggaaaaatgt gctcacctcc gtttctccgg gacaattgat ggtaaaaatt gtaagtgatg
     7621 aattgacaac gctgatgggc ggaacagtta cagaaatcaa tgtgaaagcc aacccgacca
     7681 tcatcttgat ttcaggctta caaggctccg gtaaaacgac tttctcgggc aagctagcca
     7741 acttcgtaaa aaccaaattg aacaaaacgc ctttattagt tgcctgcgac gtgtatcgcc
     7801 ctgcagctat caatcagctc acggtgttgg gcgagcaaat tggtgtggag gtatattcgg
     7861 aaattgaaaa taaaaatcct gtacagattg cgcaaaatgc tattgcacat gccaagcaaa
     7921 aaggtcagaa tgtcattatc gtggatactg ccggccgctt ggcggtggat gaagaaatga
     7981 tgaacgaaat ttacaatgtc aagcaagcga tacaaccaca agaaaccttg tttgtagtgg
     8041 attccatgac aggacaagat gcagtgaata cggcaaaagc tttcaacgat aaaataaatt
     8101 tcgacggcgt tgtcttgaca aaattagacg gcgatacgcg cggtggcgcg gcgctttcta
     8161 taaaatatac ggtggacaaa ccgataaaat ttgtatcgcg cggcgaaaag ttagacacgc
     8221 tggatgtttt ttatcctgag cgtatggcgc agcgtatctt gggcatgggt gatattgttt
     8281 cgtttgttga aaaagcacag gagcaatttg acgagaaaga agcgcagcgt ttatccaaaa
     8341 aaattgccaa aaaccagttt gattttgatg atttcttgca gcaaatcaat caaatccgca
     8401 agatgggaaa tatcaaagat ttgttgggca tgataccggg tgtgggcaag gctataaaag
     8461 atattgacat cgatgataat gcatttaaaa aaatagaggc aatcattttt tccatgacac
     8521 ccgaagagcg caaaaatccg gatattttaa acggcaagcg cagggagcga ctggctaaag
     8581 gttcaggaaa ttccatacag gatgtcaata attttatcaa gcaattcgac cagatgaaat
     8641 ccatgatgaa gaaatttcaa ggcatgggca tgaagaagta atttgagttg tatttagttt
     8701 attagttaaa tagtgtaatg gttaattagt tcgtatttga caaatttgac aaacttaact
     8761 catcaaaaaa ttggtacatt gttatatcga taaattggca cattagtaca tcaacaaatc
     8821 actacattaa tacatcgata aattggtaca ttggtacatc aacaaatcgg tacatcaata
     8881 aattcgtata ttcgtttatg aaacaattcg gtattatcgg ttttccgctt tcgcattcat
     8941 tttcgcccgg ctattttgcc gcaaagtttg aatgcgaaga tattacggat gcaaactatg
     9001 ccgtttttcc gcttgaaaaa atagaatatt tcaatgattt atgtcttgag tttcccaact
     9061 taaaaggctt gaatgtaacg ataccttaca aagaaaaaat cattgcgtat ctggatgaac
     9121 tttcggagga agcccaaaaa attgaggctg tcaatactat tttgttttct gacggaaaaa
     9181 aaacaggcta taatacagac atttacggtt ttgaaaacag cctccaaccg ctgctgaaca
     9241 acacccataa acaagcactc attttaggca ccggaggtgc agcaaaagcc gtagagtacg
     9301 tgttgcaaaa attgggaatc gggtataggc atgtttctcg gcatgaaact gaaaatgcct
     9361 actgctacga agacctgttt gacaatgaca gtatattgca agcacatact ttaattatca
     9421 atacatcacc aattggcatg tacccgaata caaatgagtg tccgaatatt ccttatgaga
     9481 aattaaccca agcgcatttg ctgtatgatt tggtttataa cccagaaaat accttgttta
     9541 tgcagtgcgg agctgcacaa ggtgcaaccg taaaaaacgg cttggatatg ctgtatttgc
     9601 aggcagaaaa aagctgggaa atatggaata aaaagcctgt ttaatttagt cattcataga
     9661 tttgaaacat tggcaacgga taaaaaatgc aattcttact tcgttttgtc attgagagag
     9721 aaatctgttt attatactgt gatttaaaat ctgcatttat atgccacgta gtggcgatac
     9781 ctttattggt ggattagtta attggtttag tagtgacatt gtccgcataa atagtcttaa
     9841 ttttcttaat gccttaatgg taaataattc gttaaaaaac aagccaagta atggtggcac
     9901 atttcatttt ggcacaagaa ccaaacgcaa tctcctgcgc cttgttcttg agctattttt
     9961 tatttttcca tatttgaagg agttagtgac gttttaatga gaatatttga atcacaacca
    10021 aatgctattt tataatttag catatctcta ataaataaac tatcattctc ataaacataa
    10081 ccaactaatg aaggcattgc gttgttataa atatttgatg ccatcacaag ttcatctaat
    10141 ctgcttgtta aatattcatt tgagtgtcca ttataatagt taaatattat tgtatcaata
    10201 acattacagt aatatgtatt tatttcttta ttatcattat tgcatgacac gtttataaaa
    10261 ataaatacaa ttaagcatat atatttcata tattatttgt taaagtattt cttaagttgt
    10321 tttgctaatt ctaattgctt tctttcatat tctgcacata ctgaacaatc ttgatattta
    10381 gatggttcgg gtataattat ttttggtgta acatcaatag ttgtagttgt tttcaggaaa
    10441 ttattgtctt ttatgttaac tgttgttttc atattctttc cattattggt tgtatatgta
    10501 aattctgtag taccaaataa aattttatct tgtgcaatat ttttcacatt tgtataacca
    10561 tcaatcattc cttgtagtct taaagcgatg tcgtgagcag tttcataatt gctaattcct
    10621 tttccctgat gttgaaagcc ttcataaatc tcatgtgcta atatagctac tcctgatatc
    10681 ccgtcatctt ttacataatc agctacttcc atatcatata catcaaaaat tgtgcattta
    10741 tttcatttcc attattctta aagactgtag ttaatttacc acttttatct ttgtgtccac
    10801 taaaagtacc tccaaaagaa atattccttt ctgattcttt taacgcataa atattagttg
    10861 ttttagttga tgttattact tcatttaatg aattataaag tgctaattgc tgtttgttaa
    10921 gttttttgga atcgcccgat aaactagata ttccttgatc atttatatta aattttactc
    10981 ctgattttcc aaatttatta tttgcaagtg tggctgcctt gtctaattct ttcttatcac
    11041 caaagaattt taaaacatct ccattaatat ctgtaaaaat aatcggatta tctccaaaac
    11101 atgaataatc actttcctaa cttttaatta tcggatcaac attccatctc cttcccaacc
    11161 ttgcatcata tgcccaaaaa ggtgcggtat taacatttcc ggcttggttt atatcgtaat
    11221 cccgttcttg accgttaaat ccataactat acttaccgca tgatatagag cttgccatag
    11281 ccatgtttaa agaatttgtc tatgccggtt tcttttatct cggtgttggt gccgtctgta
    11341 tttttggtat atactctgta aaaataaaca ccatttgcca gcctgtcgcc atacatatcg
    11401 gtgccatccc atttaaaatc ggtaacattc gtgccgatat gcaagttgcc cagttcttct
    11461 ttgctaatct cacgcactac tttaccactg gcactgaata tctgtatcat cagtttatcc
    11521 ggtacttgct gtccggtaat ggtaaataca aactgtgtgg aagtactgaa aggattcggg
    11581 taattgagaa catgcgtaat tgttggtttg gagtccactt taaatttaat acggtattcg
    11641 tattttccgg catcattgcg actgcggtct ataccttgtg ctctcagctc ataggtgccg
    11701 tcttgaagcg ttggcttata gatgaggcgg gcttcattgt tacgactcaa attacttggg
    11761 tcggccggta taaatgtaat atcagattgt tgcaaatcga ctaaaactgg cgtggtagga
    11821 gcgttggggt aatagaggta taacaaaaat tcgttagtgt catttaaagc cagatatttg
    11881 ttttcgtctt tcagcctcaa taaaatttcc ggtgtggaag aaacatattc cccatcggtg
    11941 atatgtctgc cgtcgaaggt tacatccagt atcgggttga ttctgtcacg taatacatat
    12001 accgtaaatt cagcaaagtt attaaaatgg aattgctcag gctggtcagt atccgggttc
    12061 acttctacgg taatcgtgtt tagtcctgta aaattgaatc ctgcaaaatc atagttaaag
    12121 ttgatgattt gatattgctt gccgggcagt ggtgcgtaac gctgatccac tactataata
    12181 tttcctgatg catctctgat agtaaaacga acgagcaagc tgtccatatt tatcggggtt
    12241 acattttcta cggcaacagc taaagagagc gtttcaccgg acacgattgt atctcttgtt
    12301 tttgaaaaat ggatagtagg attgacaaca gcttccggaa ctttatcata agtaacacgc
    12361 caaaagtcca tttgaggtgc tgtgccgaat gaatcatctt gcgtattcca ggtaagctga
    12421 ataaaaggtg tttttttagc atcaatgctg ctgatatcta tgggaatacc ttttaataaa
    12481 ttatccatat aaatagtgct tgtacccgaa gtatcaaaac ctctgatgct tacattggaa
    12541 taatcgtaat atggattttc caaggcatgc caatccattt gaaaagtttt ccagttttta
    12601 gccggcccaa taatagtaga ttgcatagta cctttattcc atgtaccact gaatgtaaat
    12661 gaagtatcaa ttatggccgc agggttattt gcagtagggg ctctgcttat ctgtatttta
    12721 ttaaatcccg gatcacagtt atggataaag aacacgaatg gttcattttc tctctgattt
    12781 cgaatagtat ttacaccaat gcgttcaaat gccgtaaata aggtctgatc taaaggagaa
    12841 gtactatcgg catcccattg agagtagccg gcattaaaga agctataacc taatacataa
    12901 gcattacaag ggatagaatt tagaaaatca ataactcgtt gtctttc
//
LOCUS       DAOQII010000044        12910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2376_length_12910_cov_4.587242, whole genome shotgun sequence.
ACCESSION   DAOQII010000044 DAOQII010000000
VERSION     DAOQII010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_12910_cov_4.587242"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLF48_03480"
     CDS             complement(<1..816)
                     /locus_tag="PLF48_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNF48418.1"
                     /translation="MLDSVIPKIKDWPINLIAKKKTKILQETISDALLEFNKTYPTNK
                     QIREQLQKTLYLERIRMKTEPWEVDPEDEQEFWGKVKKQLIQSDPSQMEEAKSEEIHQ
                     EILTSIITRYANEIWGNFNPNLFWIVRQLLRRIFARLFNSHFGGFRGLFNPGDKLKDK
                     LIIAGPVDKLRNLVTKGTIILVPTHFSNLDSVIIGFGMDYIGLPAFQYGAGLNLFNSK
                     FFSFFMGNLGAYKLDRRKKNPIYLETLKSYSRANLVAGAHTIFFPGGTRSRAGN"
     gene            complement(917..1837)
                     /gene="xerD"
                     /locus_tag="PLF48_03485"
     CDS             complement(917..1837)
                     /gene="xerD"
                     /locus_tag="PLF48_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNF48419.1"
                     /translation="MIVQSTVYWKSYLDNFSAHLKVEKSLSPNSVDAYLHDVRLLTQY
                     LTVENKTVNPEDLHLQDFEHFLAYLHDLGLNDRSQARIISGLKAFYKYLVNDEFIKDD
                     PTVLLEGPKLSKKLPDTLSYEEIEKLLQALDLSKDENVRNRAMLETLYSSGLRISELI
                     NLRISHIYEDVGFIRVIGKGNKERLVPIGNDALKHINLYREHVRNKMSIHPKYSDILF
                     LNRRANPITRVMVFIVIKKLAEQIGLNKNISPHTFRHSFATHLIEGGADLRAVQQMLG
                     HASITTTEIYTHLDQQYLRESLTLHHPRFK"
     gene            complement(1951..2700)
                     /locus_tag="PLF48_03490"
     CDS             complement(1951..2700)
                     /locus_tag="PLF48_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48420.1"
                     /translation="MKSIPLYICLILIFITACKRHKDQIAEHPKNLALIIGDPVDYGM
                     DSTVMFPIGANYNPKIVKNEESEIGGGSFNAISFKKNTSTLYDKRASSEFINSNTSDF
                     DIRNILFYDKVSGNSFPITTDTLHILSFAIHSEFRKPLIFFRIVKADLNKDGIYDNRD
                     AVMLYTSGLNGRQFTKITPENEHFLDYFYYPETNKILIKTSIDIDKDNKFSEKDETNF
                     REMHLLNTQFGREIFTNSLKDSLKMQINALK"
     gene            complement(2809..3951)
                     /locus_tag="PLF48_03495"
     CDS             complement(2809..3951)
                     /locus_tag="PLF48_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF48421.1"
                     /translation="MKSIKSMLLLCVLAVALNGYAQKGKVNSAEISLSEGKVLDAKKD
                     IDAALTDAETQNSVKAWRVKGDVYKDIYETKLYYPQNPNCLFDAKDAYIKALGLETNP
                     KKQKDYSTPLSALEGYLFNEGLSRYKQNNNEDAFRHFSTAYSINELLLSKGLQKSIDT
                     SALFAAAVSGASAKKYTEVTPMLEKLVSMNYNEPSVYESLAQIYEETKNTEALAEVVK
                     KGLDKFPDNKNLKVLELNQALSSNDLSAQIQKFEDAVKKEPKNTSYMFNLAVIYDKAG
                     QVDKAKATYEQIIAADTKFGDAYYNLGIMTFNEGIELNKKMNALDDKEVDKYNALKKQ
                     RDEIFQKALPYLEKAYQIDTKNPDYKQSLRKVYAAMNMLDKAKALD"
     gene            complement(3974..6544)
                     /gene="gyrA"
                     /locus_tag="PLF48_03500"
     CDS             complement(3974..6544)
                     /gene="gyrA"
                     /locus_tag="PLF48_03500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNF48422.1"
                     /translation="MAEELEDEKGSRIIPINIEDEMKTAYIDYSMSVIVSRALPDVRD
                     GLKPVHRRVLYGMNELGLNAGKPYKKSARIVGEVLGKYHPHGDSSVYDTMVRMAQPWS
                     LRYPLVDGQGNFGSIDGDPPAAMRYTEVRMRRIAEEMMEDLDKETVDFQLNFDDSLEE
                     PTVMPTQIPQLLVNGASGIAVGMATNILPHNLTEVIDGIVAYIDNREITIDELMNFVK
                     APDFPTGGIIYGMEGVRDGFRTGRGKVRVRGRATIEESKEGRMQIVITEVPYQVQPKS
                     IITRVDELVSDKKLEGISEARDESDRDGLRVVFDLKRDANANVILNNLYQHTPLQTSF
                     GINNIALVNGRPQTLNLKDLIRHFVDFRLEVVTRRTQYELRKAEERLHILEGYLIALD
                     HLDEVIKLIRNSKNPEEAKDGLINNFGMSEIQAKAVLALTLQRLTGMEIEKIREEHRE
                     ISALISRLKEILANEFMRFDIIKSELAEIKRKYGDERRSEIVYYGDDINYEDMIAEEE
                     MVVTISHLGYVKRTSLDEYKRQNRGGKGSFGGKTRDEDFIEHIFVASTHNYLLLFTEQ
                     GKCFWLKVYEIPEGNKTSKGRPVQNIVNINLEDKIKAFINIKDLKDEEFLDSHFIVMA
                     TKKGTVKKTSVREYSRPRQNGVNAITVREGDELIDVALTNGQSEILLGSKFGYAVRFD
                     EEDARAIGRTASGVRGITLRAEDDEVIGMVCVDKTLETKPDIMVVSENGYGKRSALDD
                     YRITSRGGKGVISLNVTDKTGSMIAIDSVTDENDLMIINKSGITIRIGLDTLRVIGRN
                     TQGVRLINVNDEDAIAAVCKIEKEEESENEGEDENNLTNDEGKDQNENGDQPSEEIKD
                     "
     gene            complement(6813..8000)
                     /gene="trpB"
                     /locus_tag="PLF48_03505"
     CDS             complement(6813..8000)
                     /gene="trpB"
                     /locus_tag="PLF48_03505"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNF48423.1"
                     /translation="MASNYTVDQHGYFGKFGGAFIPEMLYPNIEELKENYLKIIESDD
                     FQIEFHQLLQDYVGRPTPLYFAKRLSEKYNTQIYLKREDLCHTGAHKINNTIGQILLA
                     QRLGKKRIIAETGAGQHGVATATVCALMNLPCVVYMGEVDIARQAPNVQRMKMLGAEV
                     RPACSGSKTLKDATNEALRDWIANPEDTHYIIGSVVGPHPFPDMVARFQSIISEEIKK
                     QLVEKTGRAYPDKVIACVGGGSNAIGAFYHFLDEDKVELYAVEAAGLGIDSGKSAATT
                     VLGKPGVLHSSKTLFMQTEDGQVIEPHSISAGLDYPGIGPVHAHLWDTKRVNFLHATD
                     MEALKAALELSRMEGILPALESAHALAALSKINHNPDNIVVINLSGRGDKDLNTYIHE
                     FKF"
     gene            complement(8124..8522)
                     /locus_tag="PLF48_03510"
     CDS             complement(8124..8522)
                     /locus_tag="PLF48_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HNF48424.1"
                     /translation="MNLSKKQHNHKHPQEQLSIFLSLACAIHCMLTPILVIALPVAGA
                     FLQKFHWIEYLIIASVILLGTSSMMHGFKYHHKNNIPVYLFFIGVMFLLIGSTIPVFL
                     PDKDLLHHVISMFGGIICSIAQFYNFKLSR"
     gene            8592..9008
                     /locus_tag="PLF48_03515"
     CDS             8592..9008
                     /locus_tag="PLF48_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNF48425.1"
                     /translation="MQKVEAILKEHDLRVTPTRVKILKTFMLQHNALSHADLETQFKS
                     EFDRVTIYRTLTSFLENGLLHRVPDDSGVAKYALCKHDGTAHNHDDDHVHFKCKKCEK
                     IECLHDLEIPRLHLPKKYIMEDANLLIEGICADCNP"
     gene            9011..9643
                     /locus_tag="PLF48_03520"
     CDS             9011..9643
                     /locus_tag="PLF48_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48426.1"
                     /translation="MKPRTADKIEHKEFEYLVERKYRNYKYVLYMFIAGLGLMYLTLT
                     LMYVLYRFEYHLPSVRLTAMFIFNNMVLLLSIVTLYLTQHFFSTSNYYQYKTCLLMVS
                     FLGLLFLAGQIIAVIKQLNLDVVNGYYTSYYLFLISIIHALQVFMSMLFWSIFSWKSW
                     KQLNHYAISLVYFTDPVMRLQLNLFSIFWYSSGLFWMIVYVFFLAYSVSI"
     gene            9746..11368
                     /locus_tag="PLF48_03525"
     CDS             9746..11368
                     /locus_tag="PLF48_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNF48427.1"
                     /translation="MRLSIVFYLVAICICNSFSQTAKYFPKPPPDTAQKKKIQIIQAD
                     SMVYTTQELGKFRKLLNHVILKHEDALMFCDSAIIDVDANYLTAYGKQVHINKSDTVQ
                     VWGDFLEYFGDQHLGRLTGSCSMRDNAMILTAPEINYNTQTDIGNYMSGGRIVNKNTT
                     ITSAIGYYYHTTGIAVFYGDVHLHDEDKNIYADSLRYDLNKEIAYFITKTKIIDKDSN
                     VIITDEGYYDTKKKKAFFTKSVELHKEDAYVKANTLDYDDEEKRSVAQGNVIFSDSSQ
                     NITLLSNYMYSEEKNSLLYAYKDPLLIKPSDNGKDTLYLSADTILTYKIAVQDTTSEL
                     DSIRILKSFYHTKIKQGEMSATCDSMYYDERDSIYKLYVKPVLWMDSTQISGDSIFLI
                     TKDKAISEVQVFNNAFIINLQDAAIFNQVKGKFITAFIENDKIKKVDVDGNAESIYFI
                     RDDNEAYIGANKSKSGFITMNFKDDKADRIKLTIAPEAVFTPMKKIQPESFRLEGFNW
                     QWNLAPKTIDDIIRDKSQYQKYLLNQQRMVTF"
     gene            11388..12746
                     /locus_tag="PLF48_03530"
     CDS             11388..12746
                     /locus_tag="PLF48_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF48428.1"
                     /translation="MKRRHFLKKTLTNLPVLIAAPTLIAASCNKEVLNPNDLTIVVVG
                     AGISGLAAAKKLKNKGFKVIVMEAQDKVGGRLRTNRTLGVAFDEGASWIHGIAGNPII
                     ALAQEAGMSSIFADDTSIKSYDMGGFTRSASTYTSAEDEFYTIIESMMSYGSTDKSFE
                     TVFNARYPDKINDRLWKFLLSAYMTFDTGDLDKLSSLLYFEGEEYTGGERLVSNGYET
                     IATYLANGLDIMLNQRVSSIDYSEKKIKVTHNALVTDADYVIVSVPLGVLKNNSISFT
                     PALPSAKQTAIQKLGMNCVNKFLLTWDSAFWDDVHYISYTPEIKDKFNYFVNVKKINS
                     SVNALMTFAYANYARQTETMTDAQVINEIMLHLKDMYGNSIPNPTHFLRTKWNSNINS
                     FGSYSYTAVGSEMRHFEDIAEEVNNKLFFCGEHTEVDYFSTVHGAYLSGIREADKIID
                     LL"
BASE COUNT         3943 a         2388 c         2297 g         4282 t
ORIGIN      
        1 attgccggct ctggagcgtg tgccacccgg aaaaaagatg gtgtgtgccc cggctaccaa
       61 attagcacga gaataagatt ttaatgtttc gagataaatg ggattttttt tgcgtcggtc
      121 taatttataa gctccgaggt tgcccatgaa aaaactgaaa aatttggagt taaataaatt
      181 taatcctgct ccatactgga aggcaggcaa accgatatag tccatcccaa atccgataat
      241 aaccgaatcg agattactaa aatgtgttgg tactagaata atagtaccct tagtaactaa
      301 atttctcaat ttatctaccg gcccggctat gataagtttg tctttcagtt tgtctcccgg
      361 attgaacaaa cctctgaaac cgccaaaatg cgaattgaat aaacgggcaa agatgcggcg
      421 taagagctgg cgaactatcc aaaataggtt ggggttgaaa ttgccccaaa tttcattggc
      481 ataacgagtg atgatacttg ttaaaatttc ctgatgtatt tcttctgatt ttgcttcctc
      541 catttgtgaa gggtcagact ggataagttg tttttttact ttgccccaaa attcttgttc
      601 atcttcagga tctacttccc aaggttccgt tttcatccgg atgcgctcca gataaagtgt
      661 tttctgcagt tgttctctga tttgtttgtt ggtaggatag gttttattaa attccaacaa
      721 ggcatcggaa atcgtctcct gaagaatttt ggttttcttt ttggctatta aattaattgg
      781 ccaatctttg attttaggaa ttaccgaatc gagcataaaa ataagtcggt gttaatttag
      841 cactttttct tcgatattgg caagtaaact ttaattcgtt gatgtaagaa aaatggatat
      901 ggcattgttt taaatcttac ttaaatctcg gatgatgcag tgtaagtgat tcccgtaaat
      961 attgttggtc gagatgcgta taaatttctg tagtagtgat ggatgcatgc cccagcattt
     1021 gctgtacggc tcgcagatct gctcctcctt ctatcaaatg ggtggcaaaa gaatgccgga
     1081 aggtatgcgg tgaaatattt ttgtttaatc ctatttgctc tgccagcttt tttatcacaa
     1141 taaataccat cacccgtgta ataggattcg cgcgcctgtt taggaagagt atatccgagt
     1201 atttaggatg gatgctcatt ttgtttctga catgctccct ataaagattg atatgtttga
     1261 gcgcatcgtt gccaatgggt actaatcgtt ctttatttcc ttttccgata acacgaataa
     1321 aacctacgtc ttcataaata tgtgagatgc gcaaattaat cagttcgctg atgcgaagtc
     1381 cactgctgta taaagtttcg agcatggcac ggttgcgtac attttcatct ttagagagat
     1441 cgagtgcctg caataatttt tctatttctt cataagaaag tgtatccggc agttttttgc
     1501 tgagtttggg tccttccaat aaaacagtag ggtcatcttt aataaattca tcattgacga
     1561 gatatttata aaatgctttt agtccggaaa tgatgcgtgc ctgactgcgg tcatttaagc
     1621 ccaaatcgtg aagatatgcc agaaaatgtt cgaaatcttg taagtgcaaa tcttccgggt
     1681 ttactgtttt attttccact gtgagatact gagtcaataa tctgacatca tgcaaatagg
     1741 catcaaccga gttggggctg agtgatttct ctactttcag atgagccgaa aaattatcga
     1801 ggtaggattt ccagtaaacg gtagattgta ctatcatgaa ttaaaagtaa caagtatctg
     1861 aactttccat ttaaaaaaga atacacaact tttaataact tgaaaccact gtggtatgat
     1921 tattttctga taagcaggat atatttaatg ttatttcaac gcatttattt gcattttcaa
     1981 cgaatctttt agtgaatttg taaatatttc tctgccaaat tgagtattta acagatgcat
     2041 ttccctgaaa ttggtttcat ccttttcaga aaacttattg tctttatcaa tatctattga
     2101 tgtttttatc aggattttat tcgtctccgg ataatagaaa taatccaaga aatgttcatt
     2161 ctcaggtgtg attttggtaa attgtcttcc atttaagccg gatgtgtaaa gcataactgc
     2221 atctcgatta tcataaatgc catctttatt caagtctgct tttacaattc taaagaagat
     2281 tagtggcttt cggaattcac tgtgaatggc aaaagataaa atatgcaaag tatcagttgt
     2341 aattggaaac gagttgcccg aaactttatc atagaaaagt atatttcgga tgtcaaaatc
     2401 tgatgtattt gaattgataa attctgaaga tgctcgtttg tcatatagcg tacttgtatt
     2461 ttttttaaat gagattgcat taaatgaacc tcctccaatt tctgactctt cattttttac
     2521 aattttaggg ttataatttg caccaatggg gaacatcact gtagaatcca tgccataatc
     2581 aacaggatcg ccaataatca aagctaagtt tttaggatgt tcagcaattt ggtctttgtg
     2641 tcttttgcat gccgtaataa atattaaaat caggcaaatg tataaaggaa tagatttcat
     2701 ctgtcaaaat tgtaaaaaac aaatctgact atgagttaaa atatattaat aaaaaatgcc
     2761 ggaactaagg gttccggcat tgaataatat tagcgtataa ttgatttatt aatctaatgc
     2821 ttttgcttta tccagcatat tcattgctgc atacactttt ctaagacttt gtttatagtc
     2881 tggatttttt gtatcaattt gatatgcttt ttctaagtaa ggtaaggctt tttggaaaat
     2941 ttcatcccgt tgttttttaa gggcattata tttatctact tctttatcat ccagagcatt
     3001 cattttttta ttaagctcta ttccttcgtt gaaagtcata atacctaaat tgtaataggc
     3061 gtcgccaaat ttagtatccg ctgcaataat ttgctcatag gtagctttgg ctttatccac
     3121 ttgtccggct ttatcataaa ttacagctaa attgaacatg taagaagtat ttttcggttc
     3181 tttttttacc gcatcttcaa atttttgaat ttgtgcactc aaatcattac tactcagtgc
     3241 ctgattcaat tcaagtactt tcaaattttt attatccgga aatttatcca atcctttttt
     3301 taccacttca gctaatgctt ctgtgttttt tgtttcttca tatatctgtg ctaaagactc
     3361 ataaactgat ggttcgttat agttcatgct taccagtttc tccagcattg gagttacttc
     3421 tgtatatttt tttgcagaag caccggaaac tgcagctgca aacaatgctg atgtatcaat
     3481 cgatttttgc aaacctttgc ttaataacaa ttcattaatg gaatatgccg ttgagaaatg
     3541 tcgaaaagca tcttcattgt tattttgctt atacctgctc agaccttcat taaataaata
     3601 tccttcaagt gcagaaagtg gagtggaata atctttttgt tttttgggat tcgtttctaa
     3661 acctaatgct ttgatatagg catcttttgc atcaaacaaa caatttggat tttgaggata
     3721 gtataatttt gtttcgtaaa tatctttata tacatctcct tttactcgcc atgcttttac
     3781 actgttttgc gtttcagcat cggtaagagc ggcatcaata tcctttttgg catctaatac
     3841 cttaccttcc gaaagtgaaa tttctgcgct gtttactttt cctttttgtg cgtagccgtt
     3901 tagtgcaaca gcgagtacac acagcagcaa catagattta attgatttca ttttttattg
     3961 tttatttaga gtactaatct tttatttctt ctgatggctg atcgccattt tcattttgat
     4021 ccttaccttc atcatttgtt aaattatttt cgtcttcacc ttcgttttca ctttcctcct
     4081 ctttttcaat tttacaaact gcggctattg catcttcgtc attaacattt atcaacctta
     4141 ctccttgcgt attacgtcct atcactctca aggtatctaa tccaattctg atagtaatac
     4201 ctgatttatt gataatcatt aaatcatttt catcggtaac cgaatcaatg gcaatcatgc
     4261 tacctgtttt atctgttacg tttaatgaaa taacgccttt tccacctctc gaagttatgc
     4321 gataatcatc taaagcggag cgttttccgt atccgttttc agacactacc ataatatccg
     4381 gtttggtttc caaggtctta tcaacacaaa ccatgccaat tacttcatca tcctcagctc
     4441 tcagggtgat tcctcttaca cccgaagcag tacgtcctat ggctcttgca tcttcttcat
     4501 caaaacgaac agcataacca aatttagaac ctaataatat ttcagactga ccattcgtca
     4561 aagcaacatc tatcaattca tctccttctc gcacagttat ggcatttact ccattttgtc
     4621 ttggacgaga gtattcgcga actgatgttt tctttaccgt tccttttttt gttgccataa
     4681 cgataaaatg agaatccagg aattcttcat ctttcaaatc tttgatattg ataaaggctt
     4741 tgattttatc ttcgagattg atattgacga tattttgtac tggacgacct ttggatgttt
     4801 tattgccttc aggaatttca tacactttca gccaaaaaca tttcccctgt tctgtaaata
     4861 aaagtaaata attgtgagtg gaagccacaa aaatatgttc gataaaatct tcatcacggg
     4921 ttttgcctcc gaaagagcct ttgcctcccc tgttttgtct tttgtattca tcaagtgaag
     4981 tgcgttttac ataacccaga tgtgaaatag ttactaccat ttcctcttct gcaatcatat
     5041 cttcataatt gatatcatcg ccatagtaca caatttcaga acgtcgttcg tcaccatatt
     5101 ttctttttat ttcagctaat tctgatttaa tgatatcaaa acgcatgaat tcgtttgcca
     5161 gaatttcttt taagcgagat attaaggcac tgatttcacg gtgttcttct ctgatttttt
     5221 caatttccat gcctgtcaag cgttgtaaag tcaatgccaa tactgcttta gcctggattt
     5281 cagacattcc gaaattattg attaaaccgt ctttagcttc ttctggattt ttagaattgc
     5341 gtatcagctt gataacttca tccagatgat ccaatgcgat taaataacct tcgaggatgt
     5401 gcaaacgctc ttctgctttg cgcaattcat attgagttct gcgtgttaca acttccaaac
     5461 gaaaatcgac aaaatgcctt attaaatctt tcagattcag cgtttgaggt cttccattta
     5521 caagtgcaat attattaatg ccaaaactgg tttgtaatgg agtatgctga tacagattat
     5581 tcaagataac attggcattg gcatctcttt tcaaatcaaa tacaacacgt aagccatcgc
     5641 gatcagattc atcacgtgct tctgaaatac cttctaactt tttatcagat accaattcat
     5701 ctactcttgt tattatagat ttaggttgca cctgataagg cacttccgta atcacaatct
     5761 gcattctgcc ctctttagat tcttcaatag tagctctgcc tcttactcta actttgcctc
     5821 ttccggttct aaagccgtct ctcacacctt ccataccata gatgattcca cctgttggga
     5881 aatctggagc ttttacaaaa ttcatcaact catcaatcgt aatttcacga ttgtcgatat
     5941 atgctacgat accatcaatg acttctgtaa gattatgcgg taatatattg gtggccatac
     6001 ctacagcaat accagatgca ccgtttacca gcaactgggg gatttgagta ggcattaccg
     6061 ttggctcttc cagtgagtca tcaaaattaa gctgaaagtc tactgtttct ttatctaggt
     6121 cttccatcat ttcttcagca atgcgccgca tacgaacttc cgtataacgc atagctgccg
     6181 gagggtcgcc atcaatagaa ccgaagttac cctgaccatc taccagcgga tatcttaagc
     6241 tccagggctg agccatacgc accatggtat cataaacgga agagtcgcca tgcgggtgat
     6301 acttacctaa tacctcaccg actatacggg ccgatttttt ataaggctta cctgcattca
     6361 aacccagctc attcatgcca tataatacac ggcggtgaac aggttttagt ccgtcacgta
     6421 catcaggaag agcacgcgat acaattaccg acatagaata atctatgtag gcggttttca
     6481 tttcatcttc aatgttgata ggaattatcc tcgagccttt ttcatcttct aattcttctg
     6541 ccataaattt ttagtatgtt taagtgcgaa gatacgaatt tagtgctgca ttttaatgaa
     6601 tcaaagtgcc taaatatcca catttttgta gttgataatc aattaattat gtcttattgc
     6661 gaccattgca taccgaagtc aaaaacaggg tgaaacattt agcactttat tgtcaaaaat
     6721 tctgttgaaa aatagcgtga aaaaatatag aaaaaatatt gaaaaaacaa acttgaattc
     6781 tctattaact acttcttata aacggaaaaa tatcagaatt taaattcatg gatataagtg
     6841 tttaaatctt tatctccacg accggataaa ttgataacga caatattatc aggattatga
     6901 tttattttac ttaaagcagc taaggcatgt gcactttcta atgccggtag tatgccctcc
     6961 attcttgaca attccagtgc tgctttgaga gcttccatat ctgtagcatg taaaaaattc
     7021 actcgctttg tatcccaaag atgagcatga acaggtccaa tgcccggata atccaaaccc
     7081 gctgaaattg aatgtggctc aataacctga ccatcttctg tttgcataaa tagcgtttta
     7141 ctactatgca gtactcccgg cttgcccaaa actgttgtag ccgcactttt acccgaatca
     7201 atgcctaagc ctgccgcctc aacagcatat aattcaactt tgtcttcatc aagaaaatga
     7261 taaaaagcac caatggcatt gctgccacct ccaacacagg caattacttt atctggatat
     7321 gcccgtcctg ttttttcgac taattgtttt ttaatttctt cagaaataat actttgaaaa
     7381 cgggctacca tgtctggaaa aggatgaggg cctacgacac tgccaataat ataatgggta
     7441 tcttccgggt tggctatcca gtcgcgaagg gcttcgttgg tagcatcttt caaagtttta
     7501 ctgccactgc aagccggacg aacttctgct cccagcattt tcatacgttg tacattagga
     7561 gcttgacggg caatatccac ttcacccata tatactacac aaggcaagtt catcagtgcg
     7621 catacagtag ccgtagccac cccatgttgt ccggcaccgg tttctgctat tatacgcttt
     7681 tttcccagcc gctgggccaa caaaatctga ccgatggtgt tatttatttt atgcgctcct
     7741 gtatggcata aatcttcacg cttcagataa atttgcgtgt tgtatttttc gcttaatcgt
     7801 ttagcaaaat agagtggagt aggtcgtcct acataatctt gaagcagttg atggaattca
     7861 atttgaaaat catcgctttc tataattttc aggtaatttt ctttcagctc ttcgatgttc
     7921 ggatagagca tttcagggat aaatgcacca ccaaatttcc cgaaatatcc atgttgatca
     7981 actgtataat tagatgccat tttttttctt acaaaataaa acaaatgcag tggatatgaa
     8041 gttttggtga tttatggatt tgttaaagaa ttgaataact atagtctgag tgaggcttag
     8101 cttttcgatt taaaaaattc tttctatctg ctgagtttaa aattatagaa ctgggctata
     8161 ctgcaaatga taccgccaaa catgctgata acgtgatgca ataaatcttt atcgggtaaa
     8221 aatacaggaa tggtactacc tatcaaaaga aacatgactc cgataaaaaa cagataaaca
     8281 ggaatgttgt ttttatggtg atatttaaat ccatgcatca ttgacgatgt acccaacaaa
     8341 ataacactgg caataatcag gtattctatc cagtgaaatt tctgcaaaaa agcacctgca
     8401 accggtaagg caataaccaa gataggcgta agcatacaat gaatagcaca tgccaaagac
     8461 aaaaaaatac ttaattgttc ttgaggatgt ttgtggttat gctgtttttt gcttaaattc
     8521 atcggtgaaa ttcagttgca aatatacaca ataatttgca acattattgc aatttatgtt
     8581 aagatactcc aatgcaaaaa gtagaagcaa tattaaaaga acacgacctg agagtaactc
     8641 ctactcgggt gaaaattttg aaaacgttta tgttacagca taatgcgctt tctcatgctg
     8701 atttagaaac acagtttaaa agtgaattcg accgtgtaac catatacaga actttaactt
     8761 catttttgga aaacggctta ttacaccgtg tgcctgatga cagtggtgtg gcaaaatatg
     8821 cactttgcaa acacgacgga acagcacaca accatgatga cgatcatgtt cattttaaat
     8881 gcaaaaaatg cgaaaaaata gaatgccttc atgatttaga aattcccaga ctgcatttgc
     8941 ctaaaaagta tatcatggaa gatgcaaatt tgcttataga aggtatttgt gcggattgta
     9001 atccataaag atgaaaccac gtactgctga taaaatagag cataaagagt ttgaatacct
     9061 ggtagaacgc aaataccgga actataagta tgtactatat atgtttatcg caggcttggg
     9121 attaatgtat ttaacactga cattaatgta tgtactatat cggtttgaat accatttacc
     9181 aagtgtccgg ttaacagcca tgtttatctt taacaatatg gttttacttc tgagtattgt
     9241 tacactttat ctcacgcagc attttttttc aaccagcaat tactatcaat acaagacttg
     9301 cttgttgatg gtgtcatttc ttggtttatt atttttggcc gggcaaatca tagcagttat
     9361 caagcagtta aacctggatg tagtcaatgg ttattatacc tcctactatt tgtttctgat
     9421 aagtattatt catgccctac aagtttttat gtctatgtta ttttggagta tttttagttg
     9481 gaaaagctgg aaacaattaa accattatgc gatatcctta gtttatttta cggatccggt
     9541 gatgcgcttg cagctcaatt tattttcgat attttggtat agttcaggtt tgttctggat
     9601 gattgtttat gtcttctttt tggcctattc tgttagcata taatattttt ttctgactca
     9661 aaagaatgaa tttaacaatt gaacttaggc aaaccaaagt tcaaatcttt tcttattttt
     9721 gagtactatt ctttgtaagt aatatatgcg gttatctatt gtgttttatc tggttgcaat
     9781 ttgcatttgt aatagctttt cccaaacggc aaaatatttc ccaaagccgc ctccagatac
     9841 tgctcagaaa aagaaaatac agattattca agctgactcg atggtatata ctactcagga
     9901 actcggtaaa tttcgtaaat tattaaacca cgttattctg aaacatgagg atgcattaat
     9961 gttttgcgat agtgcaatca tcgatgtgga tgccaactac ctcacagctt atggcaaaca
    10021 agtgcatatc aacaaaagcg ataccgttca ggtttggggt gattttttag aatactttgg
    10081 tgatcagcat ctgggaagat taaccggttc ttgttcaatg cgagacaatg cgatgatatt
    10141 gaccgctccc gaaatcaact ataacacaca aaccgatatt gggaattata tgagtggcgg
    10201 aagaattgta aataaaaaca caacgattac cagtgctata ggttactatt atcacactac
    10261 cggaatagct gtcttttatg gcgatgttca tttgcatgac gaggataaaa acatttatgc
    10321 cgactcattg agatacgatt taaataaaga gattgcctat tttattacta aaactaaaat
    10381 cattgacaag gattcgaatg ttatcattac cgatgaaggt tattacgata cgaaaaagaa
    10441 aaaagcattc tttaccaaaa gtgtggaatt acacaaagaa gacgcttatg tgaaagccaa
    10501 tactttggat tatgatgatg aagaaaaacg ctcagtagcg cagggcaatg taattttcag
    10561 cgattcttcg caaaatataa ccttgttgag caattacatg tattccgaag aaaaaaacag
    10621 tttgctgtat gcttacaaag atcctttgct tattaaacct tctgacaatg gaaaagatac
    10681 gctctatctg agcgcagaca caatattgac ctataaaata gcagtgcagg atacaacatc
    10741 tgaactagat tccatacgta ttttaaaatc attttatcat acaaaaataa agcagggtga
    10801 aatgagtgcc acatgtgatt cgatgtatta tgacgaacgt gattcgattt ataaactcta
    10861 tgtaaaacct gttttatgga tggactcgac acaaataagt ggagattcta tttttctgat
    10921 tacaaaagat aaggctatca gcgaggtaca agtgttcaat aatgccttta ttatcaattt
    10981 gcaagatgct gctattttta atcaagtaaa agggaaattt attaccgcat ttatagaaaa
    11041 tgataaaata aaaaaagtgg atgtagacgg aaatgctgaa agtatttatt ttattcgtga
    11101 tgataacgag gcctatatcg gtgctaacaa atcaaagtca ggatttatta cgatgaattt
    11161 caaagacgat aaagcagacc gtataaagtt gactattgct cccgaagctg tattcacacc
    11221 tatgaagaaa atacaacccg aatcatttcg actggaaggc tttaactggc aatggaatct
    11281 tgcgccaaaa actatagatg acataatacg ggataaaagt cagtatcaaa aatatttgct
    11341 gaaccaacaa cgtatggtaa cattttaaac tcattattaa attaaagatg aaaagacgcc
    11401 atttcttaaa aaaaacattg accaatttgc ctgtattaat cgcagcacct acattaatcg
    11461 cagcatcttg caataaagaa gtactcaacc cgaatgattt gaccatagta gttgtaggtg
    11521 caggcatatc gggacttgcc gcagccaaaa aacttaaaaa caaagggttt aaggttatcg
    11581 tcatggaagc acaagataag gtaggcggca gattgcgaac caatcgcact ttgggtgtgg
    11641 catttgatga aggtgccagt tggatacacg gcattgccgg aaatccaata attgcgcttg
    11701 cacaagaagc aggaatgtct agcatttttg cagatgatac gagtattaaa tcttatgaca
    11761 tggggggctt cacaagaagt gcaagtacgt atacttcagc agaagatgaa ttttacacga
    11821 ttattgaaag catgatgagt tacggcagta ccgacaagag ttttgaaacg gtctttaacg
    11881 cgcgttatcc agacaaaata aatgacaggc tttggaagtt cctgctttca gcctacatga
    11941 cattcgatac gggagattta gataaactat cctctctctt atattttgaa ggagaagaat
    12001 atacaggtgg cgaaagactt gtaagcaatg gatacgaaac aatcgccaca tatttggcaa
    12061 acgggttgga tatcatgctc aaccagcgcg tgagcagtat tgattattcc gagaagaaaa
    12121 taaaagtaac gcacaatgca cttgttacag atgccgatta tgtaattgtg agtgtaccgc
    12181 ttggtgtatt gaaaaacaac agcatctcat tcactcctgc attaccgtca gccaaacaga
    12241 ctgccatcca gaaattgggc atgaattgtg tcaataaatt tttactgact tgggatagtg
    12301 cgttttggga tgatgtacat tacatttcat atacgcctga aatcaaggat aagtttaatt
    12361 atttcgtgaa tgtcaaaaaa ataaattcgt cagtaaatgc gttgatgact ttcgcttacg
    12421 cgaattatgc acgccaaacg gaaacaatga ctgatgcaca ggttataaat gaaatcatgc
    12481 tgcatttgaa agatatgtat ggcaacagta tcccgaatcc tacgcatttt ttgaggacga
    12541 agtggaatag caatatcaat agtttcggat catattctta tactgcagta ggctcagaga
    12601 tgcgtcattt tgaagatatt gcagaagagg tgaataataa gttgtttttt tgcggtgagc
    12661 acaccgaagt agattatttt tccacagtgc atggcgccta tttaagtggc atccgagaag
    12721 cagataagat aatagattta ctttaattat ggatgataaa tcacaagcct tcaggcagcc
    12781 tttagtaacc gcaaccggaa ttattttagg ctttatctta aacttctcag ccacatttgt
    12841 gaagtctgaa agtattgtat ctgatttagt ttcctacctt ataggtgtat ttatgctgac
    12901 cggcataata
//
LOCUS       DAOQII010000045        12611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2457_length_12611_cov_5.318812, whole genome shotgun sequence.
ACCESSION   DAOQII010000045 DAOQII010000000
VERSION     DAOQII010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12611
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2457_length_12611_cov_5.318812"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..2975)
                     /gene="uvrA"
                     /locus_tag="PLF48_03535"
     CDS             complement(87..2975)
                     /gene="uvrA"
                     /locus_tag="PLF48_03535"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNF48429.1"
                     /translation="MQSLPPISQEEITHINIYGAKEHNLKNIEVHIPRNQFVVITGLS
                     GSGKSSLAFDTIYAEGQRRYMETFGTYARQFVGSMERPDVEKISGLTPVISIEQKTTN
                     RNPRSTVGTTTEIYDFLRLLFARAGTAYSYNTGKKMVRFSEEQIIDDILLKFLHKKIA
                     ILAPVVRGRKGHYRELFEDIRKQGYLRVRVDGVTQEIQAKMQLDRYKIHDVEVVIDRI
                     KVDTEETSGKKTESTLQRLRQSVNKALKEGKGLLFIEDLETGSLSTYSKTLMCLETGL
                     SYEEPSPNTFSFNSPYGACPECKGLGVIKTADLEKIIPDDKKTIREKGLAPLGEYRDT
                     YTYKMVEHILKEFKTNLDTPIYQLPENCLQTLLYGYDKSIKVEARDNKERKSTNTHWT
                     LSYEGIVHLINKSFLDNSSDALRHWAENYVSETDCTTCNGTRLKKESLWFKLGEKNIS
                     EISNYDLNELLEWVKILPQQLSKNEQTIAKDILKELEDRIGFLLDVGLNYLNLNRATG
                     TLSGGESQRIRLASQIGSQLMGITYILDEPSIGLHQRDNQRLISALRNLSDIGNTVIV
                     VEHDKDIMLASDYILDLGPRAGEHGGEIVAAGTPKDFLKQKTTTAKFLSHELNIEIPK
                     IRRKGNGHFLKLFGASGNNLKNVSIEIPLGTFTCISGVSGSGKSTLINETLYRILNQH
                     FYRSPFNPMPYKNVEGLEFLDKVIEIDQSPIGRTPRSNPATYINVFDKIRDLYAALPE
                     SKIRGYKTGRFSFNVIGGRCEECQGGGMKLIEMNFLPDVYVECPKCLGKRYNRETLEV
                     KYKGKSIYDVLEMPIEDAVHFFENIPSVYRKFQTLVDVGLGYVRVGQSATTLSGGEAQ
                     RVKLSEELSKRDTGKTIYILDEPTTGLHFEDIRVLLQVLQKLVAKGNTVVVIEHNLDV
                     IKVADYLIDLGPEGGKFGGEVVAVGTPEQVAKNKKSITGQYLKEELKL"
     gene            complement(3037..3597)
                     /locus_tag="PLF48_03540"
     CDS             complement(3037..3597)
                     /locus_tag="PLF48_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNF48430.1"
                     /translation="MKTYISILRGINVSGHKLIKMDELKKTAGEIGFEQISTYIQSGN
                     LVFKSKLNDTSRISKLLSEAIKQKFGFEVPVITMSKEELQQIIKLNPFVLQKELDQKY
                     FHLTILDSCVASIYADIFEKIDLKNDKAVGVDRVIYLYCPDGYSNTKLSNTYIENKLK
                     VKATTRNWKTINELNRIADAVSYLKT"
     gene            complement(3801..4301)
                     /locus_tag="PLF48_03545"
     CDS             complement(3801..4301)
                     /locus_tag="PLF48_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48431.1"
                     /translation="MKRITFLLALILWLGHAIAQPPPPPPGEFEEGKPGNEKIEAMKV
                     AFITKMLKLTPEEAQKFWPVFDQYEQEKRELRKSTLGSIKELKDDGDFTAAEAETAIS
                     QYVLFKTKELDLLKKYIALFRNILPAQKVAKLISAEDRFKKMLAKQAQQGGPGGMPPK
                     GKKPPK"
     gene            complement(4319..4963)
                     /locus_tag="PLF48_03550"
     CDS             complement(4319..4963)
                     /locus_tag="PLF48_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48432.1"
                     /translation="MKTNKEITEELKAISPRLLQIKHDEIPLIISENYFGQLQDEVLL
                     RISEENGKLAQLDKNQPLVPDAYFDTLGDKVMNRIATEKIEDPPLTVHKNQHDKKNKL
                     FYSFRKLSIAACVVALLGIGIYKVVQKPEKELICIDGIACLTQDEILQYMQVHSHEFE
                     LDDVQKAVSNTLKTDSVATGKKQLNEKEEKAIDEFIRQDTYTFDELESADTDIF"
     gene            complement(5008..5535)
                     /locus_tag="PLF48_03555"
     CDS             complement(5008..5535)
                     /locus_tag="PLF48_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF48433.1"
                     /translation="MTDDEILAQCRSEETKEKGFRNLMNAYQQRLYYHIRRFVNTHED
                     ADDVLQNVFIKIWKNIDAFRSESSLYTWVYRIASNESITYINKQKRTATYTLEHTAAI
                     YKTGSDNENPEQVQLRLQAAINTLPDKQKQVFIMRYYDEMPYEEMSKILETSVGALKA
                     SYHHAVKKIEEIITA"
     gene            complement(5669..6595)
                     /locus_tag="PLF48_03560"
     CDS             complement(5669..6595)
                     /locus_tag="PLF48_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNF48434.1"
                     /translation="MKNKLTYFLLGVSACLVFSLFVNAAGVGNKYFSFIKKGSGENLA
                     MPVQTTFAVKLPESVSFAGEAVPLDDPEVRERLDRELTVNAYWQSATIFLMKRANRFF
                     PTIEKILTEEGVPQDFKYLALAESGLDNVVSSAGAAGYWQFLKSSGIQYGLTINDEID
                     QRYDIEKSTRAACKYLKYAYGQTGSWTAAAGGYNMGISGISKQQALQGETAYYHLLLN
                     SETSRYVQRIVALKIIMENPKDYGFYLTPEDLYPPFKYTTQTVSSKTNWVEFAKSNNI
                     SYKLLRVYNPWIRDMNYTNKDKGSYEVKIPQL"
     gene            complement(6592..7104)
                     /locus_tag="PLF48_03565"
     CDS             complement(6592..7104)
                     /locus_tag="PLF48_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HNF48435.1"
                     /translation="MEIRNKVAESGIITIDLESFLPTDEIIELDIKAMLFQGLLLKEK
                     DFRETLKKTDWSVYANKTVALFCSTDAIIPQWAYMLLTNYLLPYTSFIFYGSKEKVEC
                     DLLLENINKTDATKYAGQKVVIKGCGTKQIPSGAYVAISKKLIPHVQSLMFGEPCSTV
                     PVYKQKKDTI"
     gene            complement(7180..9339)
                     /locus_tag="PLF48_03570"
     CDS             complement(7180..9339)
                     /locus_tag="PLF48_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNF48436.1"
                     /translation="MALIGKIRERSWILVGFIALALFIFIIEAAFNSGSRLFGNRKTD
                     YGEVNGKAIQGSDYESALTDYKESIKAINPNIQFTDQIESQLKEDVWTTMIAKQLLGK
                     SYNQLGLEPTPNELKELMFGDRPHPIAQQFIYQLARSNPDIVDQNTGKLNQGKIRELM
                     TNLDKIDEQNGTNLRTTIRSIENLIKVDAVKQKYATLVSQSFYVPTFMVNEVLSSAKS
                     RKASIVNVPYTTLKDDNYKVTDAEISAYIDKHKAKFQMDASCVMDVVKFDILPSAADS
                     ADAYKTISQRRTEFLAAAPKDSAFIARYSEQGANVDYYTEDEVRRSSRNADTLFNLPV
                     GTLTNIYTQGSYYMFTKIIDRRMAPDTVRAAHILLSLGKTDAENEAAYKKADSLIQQV
                     ASGKINFGQAAADNSLDEGSKKKGGDLGYFSRGQMVKPFNDKVFFEMKPGQIAKVESQ
                     FGLHIILLIDAKAPKVMTKFADFATEIAPSDATITDIESTAQTFYSKNRTAKDFDKNA
                     KTENLIKDVVVTKSTYEVPQLGSARKLVQWAFDQKKTDEVGFTDYADKYVVSKLTRII
                     PQGLAKPADVKDEVTTLVRNEKKGKDLVAKLNAASSGATDLSSIAAKVEGAVVNDTAL
                     LRFNAAFVPGIGQEPALVGTAFGVNIGQVSKAVAGNENAFIVKVNAEEPAPQQTEDIN
                     LYKQMFAQRFASRINFQSIMESIMKKKKVEDLRYKFF"
     gene            9709..11607
                     /locus_tag="PLF48_03575"
     CDS             9709..11607
                     /locus_tag="PLF48_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNF48437.1"
                     /translation="MSDIIKHECGIALIRLLKPLSYYKEKYGDIHYGLSKMYLLMQKQ
                     HNRGQDGCGLATIKLDTEPGLAYFDRERSVDKQAIQAIFDKILYPFSQLTTREKELVQ
                     NMDYVKKHYKFMGELLLGHLRYGTHGGNDMSNCHPRIRANNWKTRTLIVAGNFNMTNV
                     DELFDNLVNLGQFPREMSDTMTVLEKIGHFLDVENQRLFKRVKPQGLSNYEITPLIEQ
                     QLNVAEILRNSAEDFDGGYAMAGMIGHGDAFVLRDPNGIRPAFYYKDDEVVVVASERP
                     AIQTTFNIHRSKVQEIKPGHALIIKKDGSVGEVECVPQKERKACSFERIYFSRGSDFE
                     IYRERKTLGKLLVPKILDAVNYDLENTVFSYIPNTAEVAFLGMSEEIQKIHIEQKLED
                     YQNGIIKSFDELQAKLTIHPRIEKIAVKDMKLRTFITEDTSRNDLVHHVYDITYGLVK
                     NNTDTLVVLDDSIVRGTTLKESILTMLDRLMPKKVIIVSSAPQIRYPDCYGIDMSKMG
                     TFVAFQAAIALWKERDEYAVLEKLYKDCLEELEKPLSEMQNLVKQVYEPFSPEEISDK
                     IAEIVKPQHIFSNVQVIYQSIENLHSACPNNTGDWYFTGDYPTQGGVRVALKSFVNYM
                     EGKNVRAY"
     gene            complement(11620..12354)
                     /locus_tag="PLF48_03580"
     CDS             complement(11620..12354)
                     /locus_tag="PLF48_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF48438.1"
                     /translation="MKLILCVLVLNYTTAFAQTLEQKFTKANQYYKDKQYDFAESAYL
                     DILKNDADNVNANYNLGNVYFHKKEYVNAVYYYEKANKFKPDDKAILHNLQMTNNLLF
                     KKQEFSSGFFLTAYLHQFIQSYTSAQWAKIALFFLWMAVVGLIFYFVKQNLFARRVGI
                     LSALMACLFFLFTYIKYNSETKKDYGILMQTSVLNKTPVLNADTKDTAQAGTKLKLID
                     TDKDWQKVQLPNDKQGWIMKSAVKEI"
     gene            complement(12402..>12611)
                     /locus_tag="PLF48_03585"
     CDS             complement(12402..>12611)
                     /locus_tag="PLF48_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48439.1"
                     /translation="QSELSKENIAEKLRAKQVDEKKITALKNTLDTCEMALFSPVEQK
                     AQMKNTFEKARMLIIDFETTLNKKA"
BASE COUNT         3614 a         2504 c         2230 g         4263 t
ORIGIN      
        1 aacaaagata ccaaatagaa gttttatatg cgcaatatcc tcgtgttaca caaaaaaata
       61 tgccgacaaa cttttttcaa tggtattcac aattttagtt cttctttcag atattgtccg
      121 gttatgcttt ttttattttt ggctacttgc tcgggtgtgc ctacggctac tacctcaccg
      181 ccaaacttac ccccttccgg tcccaagtct atcagataat ctgccacttt tattacatct
      241 aaattgtgct caataaccac aacggtatta cctttggcaa ccaacttttg caacacctga
      301 agcaataccc ggatatcttc aaaatgcaag cccgtagtag gttcatccag aatataaatt
      361 gtttttccgg tatcgcgctt acttagctct tccgataatt ttacacgctg cgcttcgcct
      421 ccactcagcg tagtcgccga ttgcccgacg cgaacatagc ccaaccctac atctaccagt
      481 gtctggaatt tgcggtaaac agacgggata ttttcaaaaa aatgcaccgc atcttctatc
      541 ggcatttcca acacatcata tatacttttg cctttgtatt tcacttccag tgtttcgcgg
      601 ttgtatcgct tgcctaagca tttcggacat tctacataca catccggcaa aaagttcatt
      661 tctattagtt tcatgcctcc gccctgacat tcttcacaac gtccgccaat gacattaaat
      721 gaaaatcgtc ctgttttata tccccgtatt tttgattcgg gcagagccgc atataaatcc
      781 ctgattttat caaaaacatt gatatacgtt gccggattac tgcgaggcgt cctgccaata
      841 ggcgactgat ctatttctat taccttatcc aaaaactcca gaccttccac atttttgtat
      901 ggcataggat tgaatggcga gcgataaaaa tgttggttta aaatgcgata aagcgtttca
      961 ttaatcaagg tggacttgcc actgcctgaa accccggaaa tacaggtaaa tgtacccaag
     1021 ggaatttcga tgcttacatt ttttaaatta tttccagaag caccaaatag ttttaagaaa
     1081 tgaccgtttc cttttcttcg tattttcgga atctcaatat tcaattcatg cgataagaat
     1141 tttgctgtgg tcgttttttg tttcagaaaa tctttaggcg ttccggcagc tacaatttct
     1201 cctccatgct cacccgcccg tggacctaaa tccaaaatat aatcggatgc cagcataatg
     1261 tctttatcgt gctcaactac tatgacagtg ttgccgatat cagaaagatt gcgcagggca
     1321 gaaatcaacc gttgattatc gcgctgatgt aagccaatgc tgggttcatc taaaatataa
     1381 gtgatgccca tcagctgtga acctatctgc gaagctaatc gaatacgctg tgactcaccg
     1441 cctgagagcg ttccggtagc acggttcaga ttgagataat ttaaacccac atccaacaaa
     1501 aaaccaatgc ggtcttcgag ttctttcaaa atatctttgg caatagtctg ttcattttta
     1561 gaaagttgtt ggggcaatat tttcacccat tccaacaatt cattcaagtc gtaattggaa
     1621 atttcgctga tgtttttttc gcctaattta aaccataaag attctttttt taatcttgtt
     1681 ccattacaag tggtacaatc tgtttcgctc acataatttt ctgcccagtg acgcagtgca
     1741 tcgctggaat tatccagaaa agatttattg attaagtgaa caataccttc atagcttaac
     1801 gtccaatgtg tattggttga tttgcgttct ttattatcac gcgcttctac ttttatgctt
     1861 ttatcataac catataacag cgtttgcaaa caattttcag gaagctggta aatgggagta
     1921 tccagattcg tcttaaattc tttaaggata tgctccacca ttttatacgt gtacgtatcg
     1981 cggtattcgc caaggggggc taagcctttt tcacgtatcg tttttttgtc atcgggaata
     2041 attttctcta aatctgccgt ttttatcacg cccaaaccct tacattcagg acatgcacca
     2101 taaggtgaat tgaaagaaaa agtatttggc gagggctctt cataagacaa accggtttcc
     2161 agacacatta aggttttgga gtaagtggat aagctgcctg tttccagatc ttcgataaac
     2221 aataagcctt ttccttcttt cagcgcttta tttacagact gcctaaggcg ttgcaaagta
     2281 ctttcagttt tttttccgga tgtttcttct gtatccactt ttatgcggtc tattaccact
     2341 tccacatcgt gaattttgta gcgatccagt tgcattttag cttggatttc ctgtgttact
     2401 ccatccaccc gcacacgcag atacccttgc ttgcggatat cttcaaacag ctcgcggtaa
     2461 tgaccttttc tgccacgtac cacaggagct agaatagcaa tttttttatg tagaaatttc
     2521 aacagaatat catcaataat ttgctcttcg gagaaacgca ccattttttt tccggtattg
     2581 taagaatacg ccgttccggc acgagcaaat aacaggcgaa gaaaatcata tatttctgtg
     2641 gtagtgccta ccgtagagcg ggggtttctg ttggttgttt tttgttcgat agaaataacg
     2701 ggagttaagc cggatatttt ttctacatca gggcgctcca tactgcccac aaactgacgg
     2761 gcataagtgc cgaatgtttc catataacga cgttgtcctt cggcgtaaat ggtatcaaaa
     2821 gccagcgatg atttgccact tccgctaagt ccggtaatga ctacaaactg attgcgcggg
     2881 atgtgaactt ctatattttt gagattgtgc tctttggcac cgtaaatatt gatatgggta
     2941 atttcttctt gcgaaatagg cggtaaggat tgcatggaat aaaactaatg aaaaatgata
     3001 agatgcggaa atgatgttga cagagctaat ttggtgtcag gtcttcaggt atgacaccgc
     3061 atctgctata cgatttaatt catttatcgt tttccagttt cgggttgttg cctttacttt
     3121 tagtttgttt tcgatatacg tattactcag ctttgtatta ctgtaaccat ctgggcaata
     3181 gagataaatt accctgtcta cacctacagc tttatcgttt tttaaatcta ttttttcaaa
     3241 aatatccgca tatattgaag caacacagga gtctaatatg gtaaggtgaa aatatttttg
     3301 gtctaattct ttttgcagaa caaatggatt tagcttaata atttgttgca gttcttcttt
     3361 ggacatggtg attacaggta cttcaaaacc aaatttctgt ttgatagcct ccgagagtaa
     3421 tttgctgatt cttgatgtat cattcagctt gcttttaaaa accaaattcc cgctttgaat
     3481 ataggttgag atttgttcaa acccaatttc accggctgtc tttttgagtt catccatttt
     3541 tatcagctta tgtccgctga cattgatacc gcgaagtatg gagatatacg ttttcatgat
     3601 gtacatacaa gataagaaaa catgagataa aaatagagcg ggggcggcgc ttcgcaccgc
     3661 ccccgcataa aaaacaaaca ttttatttgc ttttcctctg ctatataaat aacgtcaagg
     3721 ttaacctttt agtatatttc tcaaaccaat cgaaccattc ttttttttgc agaattaagt
     3781 aagccagtaa aaggtgctgc ttatttcggt ggtttctttc ctttaggtgg cattcctccc
     3841 ggcccgccct gctgtgcctg tttcgcgagc atcttcttga agcggtcttc tgcactaatt
     3901 agttttgcaa ctttttgggc aggcaaaata tttctgaata aggctatata tttttttaat
     3961 aaatccaatt ctttagtttt aaataataca tattgactaa tagcagtttc tgcttcagct
     4021 gcggtgaaat caccatcgtc tttgagttcc tttatactcc ccaacgtgct tttgcgcaat
     4081 tctcgtttct cttgctcata ctgatcgaat actggccaga atttctgagc ctcctcggga
     4141 gtcagcttta gcattttggt tataaaagct actttcatcg cttctatttt ttcgttaccc
     4201 ggttttcctt cttcaaattc tccgggtggt ggaggtggtg gttgagctat ggcatgtccc
     4261 agccataata ttaatgccaa taaaaatgtg attcttttca tggttacaag tttaattttt
     4321 aaaaaatatc agtgtcagcg ctttctaatt catcaaatgt atacgtatcc tgtcgaataa
     4381 attcgtcgat tgctttttct tctttttcgt ttaattgttt ttttccggta gcaacactat
     4441 ccgttttaag tgtattactt actgcctttt gcacatcatc caactcaaat tcatggctgt
     4501 gcacttgcat atattgtaaa atttcatctt gagtaagaca ggctataccg tcaatacaaa
     4561 tcagctcttt ttccggcttt tgtactactt tataaatacc aattcccaat aatgcaacta
     4621 cacaagcagc tatactcagc ttacggaaac tatagaacag cttattcttt ttatcatgtt
     4681 gatttttatg aactgtaaga ggtgggtctt caattttttc cgttgctatt ctattcatta
     4741 ccttatctcc cagtgtatca aaataggcat caggaactaa tggctgattt ttatctaatt
     4801 gtgccaattt tccattttct tcgcttatgc gcaataaaac ttcatcttgc aattgaccga
     4861 aataattttc agaaataatc aaaggaattt catcatgttt tatctgtaac aagcgtggtg
     4921 aaatggcttt cagttcttct gttatttctt tatttgtttt catcttttac tctgtatgtt
     4981 tgacaatact attcagctaa agtttaatta tgcagtaatt atttcttcaa tttttttcac
     5041 ggcgtgatga taacttgctt taagtgcacc aacagaagtt tcgagtatct tgctcatttc
     5101 ttcgtaaggc atttcatcat aatatcgcat gataaaaact tgtttctgtt tgtcgggcaa
     5161 ggtattgatg gccgcttgta aacgcaactg aacttgttca ggattttcat tatcgctgcc
     5221 tgttttataa attgcagctg tatgttctaa tgtatatgta gcagttcgtt tttgtttatt
     5281 gatgtaggta atagattcat tgctggcaat gcgatacacc caggtataca atgagctttc
     5341 tgagcgaaag gcgtcaatat tcttccatat cttaataaat acattttgca aaacgtcgtc
     5401 agcgtcttcg tgggtattta caaagcggcg aatatgatag tacagacgtt gctgataagc
     5461 attcatcagg ttgcgaaagc ccttttcttt tgtttcttca gaacggcatt gcgccaaaat
     5521 ttcgtcgtct gtcatcacca tattttgctt agacgactca aaatacaaaa ggtttaatct
     5581 taagcatgtt ttttttgtac aatttatacc ctgcaaaaat caagatggct aatgccacaa
     5641 taatgataac caaggtataa taccaccttc aaagttgagg tattttcacc tcgtagctgc
     5701 ctttgtcttt attggtatag ttcatatcac gaatccatgg attatacacg cgcaacaatt
     5761 tgtaactgat attattactc tttgcaaatt ccacccaatt ggttttggaa gagaccgttt
     5821 gtgtagtgta tttaaatggc gggtataaat cttctggagt aagataaaag ccatagtctt
     5881 taggattttc cattataatt tttaaggcta cgatacgttg tacataacgg ctggtttctg
     5941 aattcaacag taagtggtaa tatgccgttt cgccttgcag agcctgctgt ttggaaatac
     6001 cggaaattcc catattatat cctcccgcag ctgctgtcca tgaccctgtt tgtccgtatg
     6061 catatttcaa atatttgcag gcagcacgtg tcgacttttc gatgtcatat cgttggtcaa
     6121 tctcatcatt tatcgtcaaa ccatattgta tgcctgagct tttcagaaat tgccagtacc
     6181 ctgcagctcc ggcagatgaa acgacattat ccaaaccact ttcggctaaa gctaaatatt
     6241 taaaatcctg cggaacacct tcttcagtca gaattttttc tatagtggga aaaaagcgat
     6301 tggcacgttt cattaaaaaa atagtagctg attgccagta ggcatttact gttaattctc
     6361 tgtccaagcg ttcgcgaact tcaggatcat caagcggtac agcttcaccg gcaaaactta
     6421 cactctccgg caatttaacg gcaaatgtag tttgcaccgg catcgctaaa ttctcgccgc
     6481 ttcctttctt aataaatgaa aaatatttat tgcctactcc ggcagcattg acaaacaagc
     6541 taaataccag acaagcactt acacccagca gaaaataagt caatttgttt ttcatattgt
     6601 atcttttttt tgtttataaa caggcacggt agaacacggt tcgccaaaca tcagtgattg
     6661 tacatgcggt atcagttttt tgctgatagc cacgtatgct ccacttggta tttgttttgt
     6721 cccacaacct ttaataacga ccttttgacc agcgtatttt gttgcatctg tcttattaat
     6781 attttctaac aacaaatcgc attccacttt ttctttagag ccgtaaaaaa tgaaagaagt
     6841 ataaggcaaa agatagtttg taagcaacat ataggcccat tgtggaataa tggcatctgt
     6901 ggagcaaaac aaggctacag tcttatttgc ataaaccgac caatctgttt tttttaatgt
     6961 ttcccgaaaa tctttttctt tcagcagcaa tccctgaaaa agcattgctt ttatatccag
     7021 ctctataatt tcgtctgttg gtaaaaaact ttccaaatca atcgtaatta taccgctttc
     7081 tgcaacttta tttctgattt ccatattttt atttcagtta aattataaaa aaagcggaat
     7141 taataaaatt ccgcttttat agaatttgtg cttatagtgt taaaagaatt tataacgtaa
     7201 atcttctact tttttctttt tcattataga ctccataatg ctttggaagt ttatacgact
     7261 ggcaaaacgc tgtgcaaaca tttgtttgta taagttaatg tcttctgttt gttgtggcgc
     7321 aggctcttcg gcatttactt ttacgataaa tgcattttca ttacccgcta ccgcttttga
     7381 aacctgcccg atattaacac cgaaagcagt tccaaccaaa gccggctctt ggcctattcc
     7441 cggtacaaat gctgcattaa aacgaagcag tgctgtgtca ttcactacgg ctccttctac
     7501 ttttgcagca atggaagata aatctgttgc acctgatgat gccgcattta attttgccac
     7561 caaatctttt ccttttttct cattacgcac caatgtggtt acctcatctt tcacgtccgc
     7621 aggttttgct aatccttgcg ggatgatacg tgtcaatttg gaaactacat atttatcagc
     7681 ataatctgtg aacccaactt catctgtttt cttttggtcg aaagcccatt gaaccagttt
     7741 tcgagccgag cctaattgcg gaacttcata agtacttttt gttactacaa cgtctttaat
     7801 caggttctct gtttttgcat ttttatcaaa atcttttgca gttctgtttt tgctgtagaa
     7861 agtttgcgcc gtagattcta tatcggtaat cgttgcatca cttggtgcta tttctgttgc
     7921 aaaatctgca aatttcgtca tcacttttgg tgcttttgca tcaattaaca atatgatatg
     7981 taaaccgaac tgagattcca cttttgcaat ctgacccggt ttcatttcaa agaaaacttt
     8041 atcattaaat ggtttcacca tttgtcctct ggagaaataa cctaaatctc cacctttctt
     8101 tttagagcct tcatctaatg aattgtcagc agctgcctga ccgaaattaa ttttaccgct
     8161 agctacctgc tggattaagc tatccgcttt tttatatgct gcttcatttt cggcatctgt
     8221 tttacctaat gacaataaaa tatgtgctgc tcttacggta tcaggagcca ttcttctgtc
     8281 tataattttg gtaaacatat aatagctgcc ttgtgtataa atattggtca aggtacctac
     8341 aggcagattg aaaagtgtgt cggcatttcg agatgaacga cgtacttcat cttctgtgta
     8401 atagtcaaca tttgctccct gttcagaata acgagcaata aatgcagaat ctttaggtgc
     8461 tgcagcaaga aattctgttc ttctttggga aatggtttta tatgcatcag ctgaatcggc
     8521 tgcagaaggt aaaatatcaa atttcaccac atccatcaca catgaagcat ccatttggaa
     8581 ttttgcttta tgtttatcga tatacgctga tatctcagca tctgtaactt tataattatc
     8641 atcttttaaa gtagtataag gtacattgac aatacttgct tttctggatt tagccgaact
     8701 taaaacttcg tttaccataa aagtgggtac ataaaatgat tgagatacca atgttgcata
     8761 tttttgtttt acagcatcta cttttatcag attttcgatg gagcgtatag ttgttcttaa
     8821 atttgtgccg ttctgttcat caattttatc aagattggtc attaattcac gaattttacc
     8881 ttgatttagt tttcctgtat tttggtctac aatatccgga ttagaacggg ctaactgata
     8941 aataaactgc tgtgcaattg gatgtggtct gtccccgaac atcaattctt taagttcatt
     9001 cggtgtaggc tctaaaccca gttgattata tgattttcct aacagttgtt tcgcaatcat
     9061 ggtagtccag acatcttctt tcagttgcga ctcaatctga tctgtaaact gaatattcgg
     9121 attaattgct ttgatagatt ctttataatc tgtaagtgca gattcataat cgctgccttg
     9181 tatggctttg ccatttactt caccgtaatc tgttttgcga ttgccaaaca aacggctgcc
     9241 actgttaaat gcagcttcaa taataaatat aaagagagcc aatgctataa atccgactaa
     9301 tatccacgaa cgctctctga tttttccaat taatgccatg tatctttaat ttaagaggtg
     9361 cgaagataca aacttgcctt atattttcaa aggagaaatg ctgattttat gctaatttag
     9421 aactctgtct ggcacgaaaa ttcagactta agttcctaag tattcgattc aatttccaaa
     9481 atactttaat taattcttat ctaattcagc tttcttaatc agttttgaga ctgatttagt
     9541 gtaaattttc ttttaaaaga gataaaagca cagttgttct tactcacaat aaattgataa
     9601 tcaatcagaa aatcagaatc tataatattt tgacattatt ttaaaatccg gcactctcat
     9661 tacgttttca aaagcgtatt tttgcatttt ctaccctgca cctgcagcat gagcgatatt
     9721 attaaacatg aatgtggcat tgctttaata cgattattaa agccactctc ctactacaaa
     9781 gaaaaatacg gtgatattca ctatggatta agcaagatgt acttgctgat gcagaaacag
     9841 cataaccggg gacaagatgg ttgcggactg gctaccataa aactggatac agagcccgga
     9901 ttggcctact ttgaccgtga acgcagcgta gataaacaag ctatacaggc catttttgat
     9961 aaaatacttt atcctttctc ccagctaacg acacgtgaaa aggagttggt gcaaaatatg
    10021 gattatgtaa agaaacatta caaattcatg ggcgaactgt tattgggaca ccttcgctat
    10081 ggaacgcacg gcgggaacga catgagcaat tgtcatccgc gtatcagagc aaataactgg
    10141 aaaaccagga cgcttattgt tgccggaaat ttcaatatga cgaatgttga cgagctgttt
    10201 gataacttgg tcaacttggg acagtttccg cgtgaaatgt cagatacgat gactgtactg
    10261 gaaaaaatag gtcattttct tgatgttgaa aaccagcgac tatttaaacg tgtgaaaccg
    10321 caagggctga gtaattatga aattactccc ttaatagaac aacaactgaa tgttgcggaa
    10381 atactgcgca attctgcgga agattttgac ggaggctatg ccatggccgg catgattgga
    10441 catggtgatg cgtttgtatt gcgtgaccca aatggtatca ggcctgcatt ttattacaaa
    10501 gatgatgaag tagtggtagt agcttctgaa cgccccgcca tccaaactac ttttaatatt
    10561 catcgcagca aagtacaaga aataaaaccc ggacacgcac tgattataaa aaaagacggg
    10621 agtgtaggtg aagtagagtg tgttcctcaa aaagaaagaa aagcctgttc ttttgaacgc
    10681 atttattttt ccagaggcag tgattttgaa atctacagag agcgtaaaac gcttgggaaa
    10741 ttattagtgc caaaaatctt agacgctgtc aattatgatt tagaaaatac cgtattttca
    10801 tacattccca atacggcaga agttgctttt ttaggcatgt ctgaggaaat acagaaaatc
    10861 catatcgaac aaaaactgga agattatcaa aatggaatta tcaaatcttt tgatgaatta
    10921 caggctaaac tgaccatcca cccgcgcatt gaaaaaatag cggtgaaaga catgaaactc
    10981 cgcaccttta tcacagaaga tacttcgcgc aatgacttgg tgcatcatgt ttatgacatc
    11041 acgtacggtt tggtaaaaaa caataccgat acgttggttg tactggatga ttctattgtg
    11101 cgggggacta ccctgaaaga aagtattttg accatgctcg acaggctgat gccgaaaaaa
    11161 gttattatcg tttcgtcagc accacaaatc cgttatccgg attgctatgg tatagacatg
    11221 agtaaaatgg gcacttttgt agcctttcag gctgctattg cactttggaa agaacgtgat
    11281 gaatatgcag tattggaaaa actatataaa gattgcctcg aagaactgga aaagccctta
    11341 agtgaaatgc agaatttggt caagcaagtg tatgaaccat tcagccctga agaaatatcg
    11401 gataaaattg cagaaattgt aaaacctcag catatctttt caaatgttca ggtcatttac
    11461 cagagtatag aaaatctgca tagtgcctgt ccgaataata ccggagactg gtattttacg
    11521 ggcgattatc ccacacaagg cggagtgcgg gtagcactca aatcatttgt caactatatg
    11581 gaaggcaaaa atgtgagagc ttattaactc tcgtttttat catatttctt ttacagccga
    11641 cttcattatc caaccctgtt tatcattggg cagttgtact ttttgccagt ctttatccgt
    11701 gtcaattaat tttaatttag ttccggcttg tgccgtatct ttagtatcag catttaatac
    11761 gggcgtttta ttcagtacgg atgtctgcat taaaatgcca taatcctttt ttgtttccga
    11821 attgtattta atgtaagtga agagaaaaaa taaacaggcc atgagtgcac ttaaaatacc
    11881 aactctacga gcaaataaat tttgtttaac aaagtaaaat attaaaccaa ccactgccat
    11941 ccataaaaaa aacaaggcta ttttagccca ttgtgcagat gtataacttt gtataaactg
    12001 atgcagatag gcagtcagaa aaaaaccgga actgaattct tgttttttaa acaagagatt
    12061 attcgtcatt tgcaaattat gtaaaatagc tttatcatcc ggcttaaatt tgtttgcttt
    12121 ttcataataa taaacagcat tcacatactc tttcttatga aaataaacat tgcctagatt
    12181 ataattggca ttcacattat ccgcatcgtt ttttaagata tccagatatg ccgactctgc
    12241 aaaatcatat tgtttatcct tgtaatactg atttgctttc gtgaattttt gttccaatgt
    12301 ctgtgcaaac gctgtggtat aattcaacac taatacgcac aagattaact tcataaatcc
    12361 ggaatttatg ccattcaaaa ccttatttat aaaaaactgc attatgcttt cttatttaat
    12421 gtggtttcaa aatcaataat cagcatacgt gctttttcaa aagtattttt catttgtgct
    12481 ttctgttcta caggtgaaaa caaagccatt tcgcaagtat ccaatgtgtt tttgagagct
    12541 gttatttttt tttcatctac ctgcttggcc ctcagtttct cagcaatatt ttctttagac
    12601 aattcgcttt g
//
LOCUS       DAOQII010000046        12605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2459_length_12605_cov_4.667570, whole genome shotgun sequence.
ACCESSION   DAOQII010000046 DAOQII010000000
VERSION     DAOQII010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2459_length_12605_cov_4.667570"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLF48_03590"
     CDS             <1..581
                     /locus_tag="PLF48_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48440.1"
                     /translation="LGTITSNNPLPVELSSFTGNCNNDNTVTLNWVTSSEINVKEFIV
                     QKSTDGFNFTQVAVLAATGNSTLPQSYTTLDADFNTEFAYYRLLIADLDGSTKLSTTI
                     LLNCSDNTSGVNIYAVEEQGIFVKTNMAVSSNSTVEVFDVAGKRIYNEQITLPQGYYK
                     HHLDMETIIADGVYIVRLNTGSKIYSQKVWVH"
     gene            complement(578..2275)
                     /locus_tag="PLF48_03595"
     CDS             complement(578..2275)
                     /locus_tag="PLF48_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNF48441.1"
                     /translation="MRNFLILLILLIFSVHVARGQNACARQKQCKIRVIGDSWAQFPY
                     VYHAYDTALAKYGFADYYAIGDATALIGMQAEVWWQFPLARFALEAALNTDAARPIEL
                     VMVSLGGNDAAFGVHATDSLSVLDDNLANSKLFMDSIFDFVHEKIPNAHIIWQGYDYP
                     NFVDALLNYPWNPYRDLWESRGEPTPFMVNRVVDYMMQHMDTTVQGYHRVGKNYIHYF
                     NCMGLMQWLYGQTTPLRVAPFGTYPPHSVPFPGGDWNYPTPYIAMGLNGLETYHLGPM
                     GYTELCNFYMRAYINAYFRRNKSASVYSMGTIYDGAVNTNGNISTGSVVVSNNGVSKN
                     KGIFSFNTEFIPDNYRVKKASLFIKNKSIQNRYTNYWNPFPDNFVLEIKKGAFGSSAV
                     EAGDFNEPSDYFDAACVVGCLQGNDYSLRFDLNDNALAFINKLGLTQFRLSINDTNVI
                     TFYNGDTTAFEGPYLDLYYDTTTVTSIKNNTLESIPFSIFPTVSRDYIKLALPTNLPD
                     EKMTISIITLDGKVAENLPLSAVKLNTIDISKLPAAEYLVQVKSSDKMYISRFIKPD"
     gene            2350..3258
                     /locus_tag="PLF48_03600"
     CDS             2350..3258
                     /locus_tag="PLF48_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48442.1"
                     /translation="MKKITSSAFFWIVLLSVIAKENVNTQLDLNEAVKSGKVKCTITG
                     NEKSTHYLQPVIVQLENTDKKSITISINNGDLFIPQDSVYQNIIVTQDALFVLKPNEK
                     KAMPVSGMCIEQFDKAGLGTTHYAYAANKNDSLKMLSAFLQKNKMQSSCAQYAMWCLA
                     NHTNINSVFSSDSTEENKLKSFLAQLTGRTFSVSDKNYKNYYFAPPKEKVSGKFNFNF
                     SNPADIQIALFNKNGILVRELYNEKNVQPGAHNFQYAFDASVYTEDIYYVKFIADNEV
                     FINREWDVKAIRDKFKQKIQQKLNGN"
     gene            3478..3885
                     /locus_tag="PLF48_03605"
     CDS             3478..3885
                     /locus_tag="PLF48_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNF48443.1"
                     /translation="MNYWLMKSEPDVFSYDDLVKNKKEKWDGVRNYQARNNLRAMQKG
                     DLALFYHSNIGKEVVGVMEIVKAAYPDPTTDDDRWVCVDVSPKKKLKNPVTLEKIKQT
                     ESLQEIPLIRHTRLSVMPVTKEEFDEIVRLGND"
     gene            complement(3897..5585)
                     /locus_tag="PLF48_03610"
     CDS             complement(3897..5585)
                     /locus_tag="PLF48_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48444.1"
                     /translation="MKKKDARVSKRLYMHFGQKHSLSFFNSGIFLFTLFYLLTLLLYF
                     ESRHSGFVTDYIGFEQNYDHCDYWHYYHCSGGKNFRYLQHAFSYPIYKYIGSDSLIWY
                     LLYCLAHALTALVGYKTLIMIFAFWNKALSKKASFFTALLFLVSPFHNEVLVWRVCIQ
                     YMTISICLFLSMRFFLLDIKKNNFKYPVASILLFIIGLLSLEQIIVMPVFIFLLSIFV
                     FIIERQKPVLTRMFSWYFLPQTGLIILYFLMSKIVYGQWVMHYGSDTFKGFNSISTVS
                     KIYNYFIKYFALVRFYPHEWKSFLFAYIECPLITLFLITCTGLGLFYLLKKFIRGNIY
                     SGTLLLMICFYIVSLIPVLQLYFSVLFYCENDRLGYLSSFFIFGIIVLILYKLPILFR
                     YFLLLILFTVNLFFAYKMSHLWKRSTEIYTSYLTAFNDYPYKQVFLLGTPDNYQGIPM
                     MRAYPSGLQEALQYRRKKPYTGKMYDVMQFNQVSKEDGMHVEQLNDSTLKVSFTHNGS
                     WFWYWGIGAKSYETPDVKVSVNQWNYIVTFKNFNRANSIILYPYQSKWMQVKWE"
     gene            complement(5610..6188)
                     /locus_tag="PLF48_03615"
     CDS             complement(5610..6188)
                     /locus_tag="PLF48_03615"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNF48445.1"
                     /translation="MHYLFLFLVCSLYFIFHSLLADLRVKSFFKKHFPFLFSYYRIIY
                     NLISILGLIILGYFTLQVTDYFILPTLFLKIIGIVFLILSLILFYIAFNAFDMWEFLG
                     ISSKHDTQYSNLIVGGIYQYVRHPLYTATIIICIALLCLHPTFGVLTFCLATFVYLET
                     GTRLEEKKLIAEFGNEYIEYQKRVKKYFPYLY"
     gene            6344..8470
                     /locus_tag="PLF48_03620"
     CDS             6344..8470
                     /locus_tag="PLF48_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNF48446.1"
                     /translation="MKKISLLVLISQILVNVFAGNPSKQIFKYPETPKINHTDTYFGT
                     EVSDPYQWLENDTSAATTAWVDAENKVTSDYLSKIPYRDKLKQRYKELYDYAKLDNPV
                     IAGKYILFNKKEGLQNQAITYIQNGINGTPEEFINPNTLDPKGLTTYEIIGISDDNKY
                     VAISVQKSGSDWQQIIIYELETKKQLADQLNWVKFSNAGWYKDGFFYSRYPKPAEGME
                     LSAKNEFHSVYYHKLGDTQDKDELVYKDPSEPLSYNSVATDEMGNYMFLYRSLGTYGN
                     EIYWKKTSEPGWDFKPLFTGNKNEYSIIDVVFDNFIVMTDEDAPNRQLISIDPLNPDK
                     KNWKKILPERKNAYLDGVYMTGKKMFSKYLENASSKLYVYDDNRKAKEIALPGAGEIE
                     VTSGRYTDTRIAYTYTSFNYPNSIFVYDIPTGYSKLFAKPALKFNPEDFVSKQVWYKS
                     KDGTKVSMFIVHKKGIVLNGKNPTLLYGYGGFSVNMSPTFSASRILLLENGGVFAMPN
                     LRGGAEYGEEWHKAGMLMNKQTVFNDFIAAAEYLIKTKYTSKQKLGISGGSNGGLLVG
                     ACMTQRPDLYKVAFPAVGVMDMLKFHKFTIGWGWVPEYGSSDQSAEMFNYLKGYSPYH
                     NIKPGVKYPATLITTADHDDRVVPAHSFKFAARLQEYGKNDVPLLIRIEKNAGHGKGK
                     PTSKIIDEQGDIWSFFFWNVGVKAIK"
     gene            8522..9409
                     /locus_tag="PLF48_03625"
     CDS             8522..9409
                     /locus_tag="PLF48_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48447.1"
                     /translation="MLPAKTEYIRIASNAFHADKIPVFLKPFWLDAVSKKWDVVIVKE
                     NDVLLAALPYCIKGNILTKRIYLPFLSFYQSVLFFQDVKQEEQQTILEQLVDQLPLTW
                     KQYFKFLPQYSNLDLSNQDYTKHGYSTYVIQDKFQLSEHHSRLYKKVIKNEYKLVETE
                     NTDQAYALLMSTFIRKNLKPYMSLHDFEKIQTVCKKFNAGKIVSCTDKQQNHLACIFY
                     AEDEQSAYYLMGGYDDQFKNSGAMVFLLHELISQAIKQKKQFNFCGSADANIARFFEG
                     FGAEKIELNIWTKGLMSRK"
     gene            9535..10494
                     /locus_tag="PLF48_03630"
     CDS             9535..10494
                     /locus_tag="PLF48_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF48448.1"
                     /translation="MWKKSSFLLTFFMSIFVCMQVHAQIFPNSTHYMINPQVKNPAFY
                     GHEDGITFGGNFRYQWAKLEGQPRTFNFFADANLPAAHGAVGLNVSADMLGAYTITTL
                     NAGYNFIQPIKNKIKICIGLNAGVVISQLDESKLTTPESTDDDLNSLKQKSLRPNLTV
                     GIALQHKFVDVGFAYSNLINFNDKFKGQSGELKTKYGNEFHAFVQGKLLFKDYIALRP
                     SISFYTDFKEFQTDLSILAGYTKYGLIGINVRGFNKKTFESISPVINITAVKNLNIVY
                     SYDLNLNKLAKVNKGTHEITLKYTLPNSKIYKQPKIINNPRFL"
     gene            10601..11908
                     /locus_tag="PLF48_03635"
     CDS             10601..11908
                     /locus_tag="PLF48_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF48449.1"
                     /translation="MNKFKFFLVTVVAITLYSCKGAKTGQDGQLVGIQERPKWDANLL
                     PYGMVYVPSGVFHAGPSDQDVNYAFNSKLKQMSIVGFYMDETEITNNEYRQFVNYVRD
                     SIAHQLLGGDHLTEGENGKQAINWEMPIDMNWETGGSATEEGSQLESMFYSEADQLGG
                     RKEMDPRKLIYEYTWFKWKEYAKEHPLLDKNAKARSEYMERKKIPIYPDELVWIRDFA
                     YSYNEPMTRAYFTHPAYDDYPVVGVTWDQCNAFCAWRTILWNNVRLSNGEARVDEFRL
                     PTEYEWEYAARGGRNISPYPWGGPYIRNTKGCLLANFKPGRGNYPEDGGFYTVNAKSY
                     WPNDYGLYNMAGNAAEWTSTAYEDDAVAFIHDKQPDFRYDAKEDEPNVMKRKVIRGGS
                     WKDIAYFLQCGTKSYEYQDSAKSYIGFRCLLPYMGRSMNDFGK"
     gene            11977..>12605
                     /gene="gldL"
                     /locus_tag="PLF48_03640"
     CDS             11977..>12605
                     /gene="gldL"
                     /locus_tag="PLF48_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNF48450.1"
                     /translation="MANFFKTKTGKNAKNMLFGIGAAVVIFGAWAKILHKPYANLMLT
                     IGLWTEAVIFLVSAFIPPEPDYYWEKIYPGLDVYDENFEKESDKHGHEVISAHLAGGG
                     ASLSGQMDKMLEAAKIDQNLVSRFGDNLKHFAENVGKMGNMTDATVASNEFANSAKAA
                     ASSLNGVKDSFTSAADGMSKLASASGDAAQYHSQVQSATKNLAALNAVY"
BASE COUNT         4424 a         2115 c         2284 g         3782 t
ORIGIN      
        1 cattaggtac gattacatcc aataatccat tacctgtaga gctatcttca ttcaccggaa
       61 attgcaataa cgataatacg gttacattaa attgggtaac ctcatctgaa atcaatgtaa
      121 aagagtttat tgtacaaaaa tctactgatg gatttaattt tactcaggtg gctgtattag
      181 ctgctaccgg taattcaact ttacctcaat catatactac acttgatgct gatttcaata
      241 cggagtttgc ttactatcga ctgcttatcg cagatttaga tggttcaact aaattatcta
      301 caaccatact gcttaattgt tcagacaata catccggtgt caatatttat gcagtagaag
      361 agcaaggtat ctttgtgaaa acaaatatgg cagtttcaag caattcgact gttgaagtgt
      421 ttgatgtagc ggggaaacgt atttacaatg aacaaatcac tttacctcaa ggatactaca
      481 aacatcattt agatatggaa accatcatag cagatggtgt ttatattgtt cgattgaata
      541 ccggcagtaa aatatattca caaaaagttt gggtacatta atccggtttt ataaatcggg
      601 aaatatacat tttatcactg ctttttacct gaaccaaata ttcggcagca ggtaatttgc
      661 taatgtctat tgtatttaat ttcacagcag acaaaggcag attttctgct actttaccat
      721 ctaaagtaat aattgaaatt gtcatctttt catcaggtaa atttgttggt aatgcaagtt
      781 ttatataatc gcggctgaca gtaggaaata tagaaaacgg gatgctttct aaggtattat
      841 ttttaatact ggttacagtc gtcgtatcat aatataaatc caaataagga ccttcaaaag
      901 cagtcgtgtc gccattataa aatgtaatca cattggtatc attaatactc aacctgaatt
      961 gggttaatcc taatttgttt ataaatgcca acgcattgtc attcaagtca aatcgaaggg
     1021 agtagtcatt gccttgcaaa caacctacta cacaagccgc atcaaaataa tccgaaggtt
     1081 cgttaaaatc cccggcttct acagctgatg agccaaacgc acctttctta atttccagta
     1141 caaaattatc cggaaacgga ttccagtaat tggtatatct gttttggatg gacttgtttt
     1201 ttatgaaaag actggctttt ttcactcgat aattatcggg gataaattca gtattaaatg
     1261 aaaaaatacc cttgtttttt gatactccat tattactgac aacgacactg ccagtactga
     1321 tattgccatt cgtatttact gctccgtcat aaatcgttcc catagaataa acactagctg
     1381 atttatttct cctgaaatag gcattgatat atgcccgcat gtaaaaattg cataattccg
     1441 tgtatcccat cggtcccaga tgataggttt ctaagccatt taatcccatg gcgatatatg
     1501 gtgtcgggta attccagtcg ccacccggga atgggacgct gtgtggagga taggtgccaa
     1561 atggtgccac tctcaggggc gttgtttgcc cataaagcca ttgcatcaag cccatacaat
     1621 tgaaatagtg gatataattt tttccgactc tgtgataacc ttgcaccgtc gtgtccatat
     1681 gttgcatcat gtaatcaact acccggttaa ccatgaatgg tgttggttct ccgcgacttt
     1741 cccatagatc tctgtatgga ttccaaggat aattcaataa agcatctaca aaattaggat
     1801 agtcgtatcc ttgccagata atatgcgcgt ttggaatttt ttcatgtaca aaatcaaaaa
     1861 tactatccat gaataatttt gaattggcaa gattatcatc caatacacta agagaatcag
     1921 tggcatgaac accaaatgcc gcatcatttc cgcctaaaga aaccatcaca agttctatcg
     1981 gtctggctgc atcagtattt aacgcggctt cgagagcgaa acgtgccaag ggaaattgcc
     2041 accatacttc tgcctgcatg ccaatgagtg cagtagcatc gccaattgca taatagtcag
     2101 caaatccata tttcgccaac gctgtatcat aagcatgata gacataagga aactgcgccc
     2161 agctgtcgcc aattactctt attttgcatt gcttttgtct tgcacaagca ttctgaccac
     2221 gagcaacatg tacagaaaaa atgagtagta taagcagtat aagaaaattt ctcatagtta
     2281 aagtttctta aatttgaaaa agtagtcaac taaatttaaa gatttacgtt tacaatcaaa
     2341 aacatagaca tgaaaaaaat tacatcatca gcatttttct ggatagtact tttgtctgta
     2401 attgcaaaag aaaatgtaaa tacacaactg gacttgaatg aagctgtaaa atcaggaaaa
     2461 gtaaaatgca ccattacagg taatgaaaaa agtactcact atttacaacc cgtcattgtt
     2521 cagcttgaaa atacagataa aaaaagtata acgattagta ttaacaatgg cgatttattt
     2581 attccacaag acagtgtcta tcaaaatata attgttacac aggatgctct ttttgtatta
     2641 aaacccaatg aaaaaaaagc aatgccggtt tcaggaatgt gtatagagca atttgataaa
     2701 gcaggcttag gcacaacaca ttatgcttat gcagctaata aaaatgattc attaaaaatg
     2761 ttgtctgcat tcttacaaaa aaataaaatg caatcatctt gtgcgcaata tgccatgtgg
     2821 tgtcttgcca atcatacgaa tatcaatagt gtattttcat cagacagtac agaagaaaat
     2881 aaacttaaat cctttttagc acaactaacc ggaagaacgt ttagtgtttc tgacaagaat
     2941 tataaaaatt attatttcgc accacctaaa gagaaagtaa gtggcaaatt taatttcaat
     3001 ttttccaatc cggcagatat tcaaattgct ttattcaata agaatggaat tttagtacgt
     3061 gaattgtata acgaaaaaaa tgttcagccc ggtgcgcata atttccaata tgcttttgat
     3121 gcatcagtat atactgaaga tatttattac gtcaaattta tagcagataa tgaagttttc
     3181 atcaacagag aatgggatgt gaaagcaata agagataaat tcaaacaaaa aattcagcaa
     3241 aaattaaatg gaaactgagg tggatattgg ttattggtga ttagttattg gtgattagtt
     3301 atttgttatt agtgttgagt tatttattgt ataataaatc tcactacttg ctacttaata
     3361 cttactactc actacttact actttattat atcttgactc ttgactctag ttttttgtat
     3421 ctcactactc actactcact acttaccact aaatgagtac ctttacaaga aaaataaatg
     3481 aattactggt tgatgaaatc tgagcctgat gttttttctt atgatgattt agtcaagaat
     3541 aaaaaagaaa aatgggatgg agtgcgcaat tatcaggcga gaaataattt gagagccatg
     3601 caaaaaggcg atttggcttt attctatcat tctaatatcg ggaaagaagt ggtcggagta
     3661 atggaaattg taaaagcagc atatcctgac cctacaactg atgacgaccg atgggtttgc
     3721 gtggatgttt ccccgaaaaa gaaactgaaa aatccggtta ccttagaaaa aataaaacaa
     3781 acagagagct tacaggaaat tccactcata cggcatacaa gactttctgt aatgccggtt
     3841 actaaagaag agtttgatga aatagtacga ttgggaaatg attagccaaa accttactat
     3901 tcccatttta cttgcatcca ttttgattga taaggatata aaataatgct gtttgctcga
     3961 ttgaagtttt taaatgtaac tatataattc cattgattta cgcttacttt cacatccggg
     4021 gtttcgtatg attttgcacc gatgccccaa taccaaaacc aactcccgtt atgagtaaaa
     4081 ctgactttta atgtactgtc attaagttgt tctacatgca tgccatcctc ttttgatact
     4141 tgattgaatt gcatgacatc gtacattttt cctgtataag gtttctttct tcgatactgt
     4201 aacgcctctt gcaagcctga aggataagcc cgcatcatgg gtatgccttg gtagttatcc
     4261 ggcgttccta aaagaaatac ttgtttgtaa ggatagtcat taaatgctgt caaataggat
     4321 gtgtaaatct cggtagagcg tttccataag tgcgacattt tataagcaaa aaaaagattc
     4381 acggtaaaca atatcagcag taaaaaataa cgaaaaagaa ttggaagctt atataaaatt
     4441 aaaacaatga taccaaatat aaaaaaagaa gagagataac ccagtctgtc attttcacaa
     4501 taaaataaaa cagaaaaata aagttgtaaa acgggtatga gagagacaat ataaaagcaa
     4561 atcatcaata acaaagtgcc ggaataaata ttgcctcgta taaatttttt taaaaggtaa
     4621 aacagaccca atcctgtaca tgtgatcaag aataatgtaa ttaaaggaca ttctatataa
     4681 gcaaaaagga atgatttcca ttcgtgagga tagaatcgta cgagtgcaaa atacttaata
     4741 aaatagttat aaattttaga aactgtagat atggaattaa atcctttgaa ggtatctgag
     4801 ccataatgca tgacccattg accatacacg attttactca ttagaaaata caaaatgatt
     4861 aagccggttt gaggtaagaa ataccaagaa aacatacgtg ttaaaaccgg tttctgtcgt
     4921 tcaatgatga atacaaaaat actcagcaag aaaataaaaa caggcatgac aataatctgc
     4981 tccaacgaaa gtaaaccgat aataaacaat agtattgacg caacaggata cttgaaatta
     5041 ttttttttaa tgtccagcag aaagaagcgc attgaaagaa ataagcaaat ggaaattgtc
     5101 atatactgaa tgcaaaccct ccataccaat acttcattgt gaaatggtga aactaaaaaa
     5161 agcaatgcag taaaaaatga cgctttctta ctcaatgctt tattccaaaa tgcaaatatc
     5221 attatcaaag ttttatagcc gactaaggca gtaagagcat gtgctaagca gtaaagtaaa
     5281 taccaaatga gagaatcaga gccgatgtat ttataaatgg gataagaaaa tgcgtgttgt
     5341 aaataacgaa aattttttcc gccactgcaa tgatagtaat gccagtaatc acaatggtca
     5401 taattttgtt caaagccgat ataatcagtt acaaagccgc tgtgtctgct ttcaaaatag
     5461 agcaataagg tgagcaaata aaatagtgta aacaaaaaaa tgccactatt gaaaaatgat
     5521 aaactgtgtt tctgtccaaa atgcatatac aaccttttac ttacccgagc atcctttttt
     5581 ttcaagattt ttttttgatg gtaaaataat taataaaggt aagggaaata tttcttcacc
     5641 cttttctgat attcgatata ctcattacca aattcagcaa ttagtttttt ttcttccagc
     5701 ctggtacctg tttctaaata aacaaatgta gccaggcaga aagtaagtac accaaacgtg
     5761 ggatgcaagc aaagcaaagc aatacaaata atgatagtgg cagtatacaa tggatgcctt
     5821 acatattgat agatgccacc aacgattagg tttgaatatt gtgtatcatg tttggaagat
     5881 atgcccaaaa attcccacat atcaaatgca ttgaaagcaa tataaaacag gatgagtgat
     5941 agaatcaaaa atacaattcc gataattttc agaaacagtg tgggtaaaat aaaatagtca
     6001 gttacttgta aggtaaaata gccaagtata attaaaccta aaatagaaat aaggttgtaa
     6061 ataattctgt aatagctaaa caagaatgga aagtgttttt taaaaaatga tttcactcgc
     6121 aaatctgcca ataagctgtg aaaaataaaa tatagcgagc ataccaaaaa taaaaaaaga
     6181 taatgcatct ttaaaaataa cacataaagt tgatgaatta gtcttgaaat acagaataat
     6241 cgaattaaat atacaaaaaa cgagatataa gcatcccaaa actaggcatt gcttcatttt
     6301 gcctgtccgt aaataaaact tatttttgtt tcaaataaac agcatgaaaa aaatcagcct
     6361 tcttgtttta attagtcaaa tattggtaaa tgtttttgca ggaaacccgt caaagcaaat
     6421 ttttaaatat cccgaaacgc caaaaataaa tcataccgat acctattttg gaacagaagt
     6481 aagcgatccc tatcaatggc tcgagaatga tacatctgca gcaactactg cctgggttga
     6541 tgcagagaat aaagttacat ccgattatct ttcaaaaatt ccatatcgtg ataaactgaa
     6601 acaacgctat aaagaattgt atgattatgc caaactggat aatccggtta ttgccggaaa
     6661 atatatcctt ttcaataaaa aagaaggact gcaaaaccaa gcaattacgt atatccaaaa
     6721 tgggataaac ggtacacccg aagaatttat caatccgaat accttagatc ctaaagggct
     6781 tactacctat gaaattatcg gtatttcaga cgataacaaa tatgtggcaa tatccgttca
     6841 gaaatccggc agcgactggc agcaaattat tatttatgaa ctcgaaacta aaaaacaatt
     6901 agcggatcaa ctcaattggg tgaaattttc aaatgcaggt tggtataaag acggtttctt
     6961 ctattcgcgt tatcccaaac cggcagaggg tatggaatta agtgctaaaa acgaatttca
     7021 ttccgtgtat tatcataaac tcggtgatac acaagataaa gatgaattgg tatataaaga
     7081 cccgagtgag ccactttcgt ataatagtgt tgccacagat gaaatgggaa attacatgtt
     7141 cttatatcgc tcattaggca cttacggaaa tgaaatttac tggaaaaaaa ctagtgagcc
     7201 gggttgggat tttaaacctt tatttaccgg gaacaaaaat gaatattcga taatagatgt
     7261 cgtgtttgat aattttattg tcatgacaga tgaagatgca cctaatcgtc aattaatttc
     7321 tatagatcca ttaaacccgg ataaaaagaa ctggaaaaaa atattaccag aaagaaaaaa
     7381 tgcctatctc gatggcgttt acatgacagg aaaaaaaatg ttctccaagt atctggagaa
     7441 tgcaagcagt aaattgtatg tgtatgatga caataggaaa gcaaaagaaa ttgcattgcc
     7501 cggtgctggc gaaatagaag ttacttccgg cagatatact gatacacgta ttgcttatac
     7561 gtatacttca tttaattatc caaattcaat ttttgtatat gatattccaa cgggatattc
     7621 caaattgttt gccaaaccgg cattaaaatt caatccagaa gattttgttt caaaacaagt
     7681 ttggtataaa agcaaagacg gcaccaaagt atctatgttt atcgtacata aaaaagggat
     7741 agttttaaac ggaaagaatc cgacactctt atatggctat ggcggattta gtgtcaatat
     7801 gtcgccgaca ttttctgcat cgcgtatttt gcttttggaa aacgggggag tatttgccat
     7861 gcccaacctg cgaggtggtg ccgagtatgg cgaagaatgg cataaagcag gcatgctgat
     7921 gaacaaacaa acagtattta atgattttat tgcagcagca gaatatctta ttaaaacgaa
     7981 atacacatca aaacaaaaac ttgggatatc cggcggaagc aacggtggtt tattagtagg
     8041 agcatgtatg acacaacgcc ccgatttgta taaagtggct tttccggctg taggtgtaat
     8101 ggatatgctt aagtttcata agtttactat cggttggggt tgggtacccg agtatggaag
     8161 ttccgatcaa agtgctgaaa tgttcaacta tttgaaaggg tattctccat accacaatat
     8221 caagccaggc gtaaaatacc ctgctacatt gattactact gcagaccacg acgatagggt
     8281 agtacctgcc catagtttta aatttgctgc ccgtttacag gaatacggca agaatgatgt
     8341 gcctttgtta attcgtatag aaaaaaatgc cggacacggt aaaggcaaac cgacctctaa
     8401 aattattgat gaacaaggcg atatctggag tttcttcttt tggaatgtag gcgtaaaagc
     8461 tattaaataa tcaattttta gttgtcttat tttcttatgc aaaaattaaa aaaattgctc
     8521 gatgcttcca gctaaaacgg agtatatccg aattgcaagt aatgcttttc atgcagataa
     8581 aattcccgtt tttttgaagc ctttttggct cgatgccgtt tcaaaaaaat gggatgttgt
     8641 aatcgtgaaa gaaaacgatg tattgcttgc cgcattaccc tattgtatta aaggcaatat
     8701 attgactaag cgaatttatt tgccatttct cagtttttat cagagcgttc ttttttttca
     8761 agatgttaaa caagaagaac aacaaactat tttagaacaa ttagttgatc agttgccact
     8821 cacctggaag caatatttta aatttttacc tcaatacagt aatctggatt tgtcaaatca
     8881 agactatacg aaacatggct attccactta tgtcattcag gataaatttc aactaagtga
     8941 acatcattca aggttgtaca aaaaagtaat taaaaacgag tacaagcttg tagagacaga
     9001 aaatactgat caggcgtatg cattgctgat gtcaactttt atcagaaaaa atctgaagcc
     9061 ttacatgagt ttgcatgatt ttgaaaaaat acaaactgtc tgtaaaaaat tcaatgcagg
     9121 aaaaatagtg agttgtacag acaagcagca aaatcattta gcctgtattt tctatgcaga
     9181 agatgaacaa tccgcctact atctgatggg cggttatgat gaccagttta agaattcggg
     9241 tgcaatggtg tttttattac atgagctgat tagtcaggct attaagcaaa aaaaacagtt
     9301 taatttctgt ggaagtgctg atgctaacat tgcccgcttt tttgaaggct ttggagctga
     9361 aaaaatcgaa ctaaacatct ggacaaaagg actgatgagc agaaaataag tgttgattaa
     9421 ggaggattgc tcaccgttca tcattcatcg ttcatagttc accgttcatt tttcgttgtc
     9481 aaatgtcctc tatccaaccc tacatttatt cattttcttt cgtatattta caccatgtgg
     9541 aagaaaagct catttttgct aactttcttt atgagcatat ttgtatgcat gcaagttcat
     9601 gcacaaattt tccctaattc cacacactac atgattaacc cgcaagttaa aaatccagca
     9661 ttttacggac atgaagacgg tattaccttt ggcggtaatt tcagatatca gtgggcaaag
     9721 ctggaaggac aacctcgtac gtttaacttt tttgcagatg ctaatcttcc ggcagctcac
     9781 ggagccgtag gtttaaatgt gagtgcagat atgttgggtg cctacacgat taccacactc
     9841 aatgccggat ataattttat tcagccgata aagaataaaa ttaagatttg cataggattg
     9901 aatgcaggag ttgttatttc acaattggac gaatcaaagc taacaacacc cgaaagtacg
     9961 gatgatgatt taaattcatt aaaacaaaag agcctgcgcc caaatttaac agtgggaatt
    10021 gctttgcaac acaaatttgt tgatgtcgga tttgcctatt ccaatctgat taattttaac
    10081 gacaaattca aaggacagtc cggtgaattg aaaaccaaat atgggaacga atttcatgcc
    10141 tttgtgcaag gtaaactttt attcaaggat tatattgcat tacgtccctc catcagcttc
    10201 tatactgatt ttaaagagtt tcagacggac ttaagcatat tggcagggta cacaaaatat
    10261 ggtcttattg gcatcaatgt aagaggattt aataaaaaaa catttgaatc aatttcacct
    10321 gtgataaata ttactgcggt taaaaacctg aatattgttt atagttacga cttaaacttg
    10381 aataagttag caaaagtcaa taaaggtacg cacgagatta ctttaaagta cacattacca
    10441 aacagtaaga tatataaaca acctaaaatc ataaataatc cacggttttt gtaaattttg
    10501 agtaaaaaat tacagttttt agcatcacaa gtgtacaact atacaataat ttgactattt
    10561 ttacatttta ttatagcttt aaataacgta aacaactgcg atgaataagt tcaaattctt
    10621 tctagttact gtagtagcta ttacgctcta cagttgtaaa ggtgctaaaa ccggacaaga
    10681 tggtcagtta gttggtattc aggaaagacc taaatgggat gccaatttat tgccatatgg
    10741 aatggtttat gtaccttcag gtgttttcca tgcaggtccg tccgatcaag atgtgaacta
    10801 tgcatttaac tccaaattaa aacaaatgtc tatagtaggt ttctacatgg acgaaacaga
    10861 aatcacgaac aacgagtatc gtcaatttgt gaattatgtg cgcgactcaa ttgcacatca
    10921 gcttttaggt ggagaccatt tgacagaagg cgagaatgga aaacaagcaa tcaattggga
    10981 aatgccaatt gacatgaatt gggaaacagg tggtagtgct acagaagaag gctcacagtt
    11041 agagtcaatg ttttattctg aagcagatca gttaggtggt cgcaaagaaa tggatcctcg
    11101 taaactaatt tacgaataca catggttcaa atggaaagaa tatgctaaag agcatccatt
    11161 attggacaaa aatgcaaaag ctcgctctga gtatatggaa agaaagaaaa ttcctattta
    11221 tcctgatgag ttagtttgga tcagagattt tgcatactct tacaatgaac caatgaccag
    11281 agcatatttt acccatccgg cttatgatga ctatcctgtt gtaggagtaa cttgggatca
    11341 atgtaatgca ttttgtgcat ggagaactat cttatggaat aatgtacgat tgtcaaacgg
    11401 tgaagctaga gtagacgaat tccgtttacc tacagaatat gagtgggaat atgctgctcg
    11461 cggaggaaga aacatctctc catacccttg gggtggtcca tatatcagaa atactaaagg
    11521 ttgtttattg gcaaacttta aaccaggcag aggcaactat cctgaagatg gaggttttta
    11581 taccgtaaat gcaaaatcat actggcctaa tgattatggc ttgtataata tggcaggaaa
    11641 tgcagcagag tggacttcta cagcttatga agatgatgcg gttgcattta tacatgataa
    11701 acaaccggat ttccgttatg atgctaaaga ggatgaacct aatgtaatga aaagaaaagt
    11761 aatccgcgga ggatcttgga aagatattgc atatttcttg caatgtggca ctaaatctta
    11821 tgaatatcag gattctgcta aatcttatat tggcttccgc tgcttattgc cttacatggg
    11881 tcgttctatg aatgatttcg gtaaataata aaattgattt ttttacgttt aaataatgag
    11941 tgtaaatttt aactaaccaa ataaaaacaa cttcttatgg caaatttctt taaaaccaaa
    12001 acaggtaaaa atgcaaaaaa catgctgttt ggtattggtg ctgcagtagt aattttcggc
    12061 gcttgggcaa aaattcttca caaaccttat gcaaacctta tgcttacaat aggtttatgg
    12121 actgaagcag ttattttctt ggtatcagca tttattccac cagagcctga ttattattgg
    12181 gaaaaaattt acccgggttt ggatgtatat gacgaaaatt ttgaaaaaga atcagacaaa
    12241 cacggacatg aagtaatttc tgcacattta gccggtggtg gcgcaagtct ttcagggcaa
    12301 atggataaaa tgctagaggc tgcaaaaatc gaccaaaatt tggttagccg tttcggtgat
    12361 aatttaaaac attttgctga aaatgtaggt aaaatgggaa atatgactga tgctacagtt
    12421 gcatctaatg aatttgcaaa ttcagcaaaa gctgcagctt cttcattaaa cggagtgaaa
    12481 gattcattta cttctgctgc tgacggaatg agtaaattag catctgcttc cggtgatgca
    12541 gctcaatatc attctcaagt gcaaagtgct actaaaaatt tagctgcttt gaatgctgta
    12601 tatga
//
LOCUS       DAOQII010000047        12549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2482_length_12549_cov_5.585961, whole genome shotgun sequence.
ACCESSION   DAOQII010000047 DAOQII010000000
VERSION     DAOQII010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2482_length_12549_cov_5.585961"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLF48_03645"
     CDS             complement(<1..670)
                     /locus_tag="PLF48_03645"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HNF48451.1"
                     /translation="MKKLTFISVLLMLLHVAIAQNIGINSTGAAPNSSAMLDVSSTQK
                     GFLLPRMTAVQRNSIASPANALIVYDTDSSALFMYNLALTKWERITPTYNFNNFIMGN
                     NAGDILVWNGANWIATPSCNIFTYLYKDNDGDGFGDACEPLLACGTTFPCYVSNKTDC
                     NDDNNSIFPGNTELCNNLDDNCNGSIDETFPLKGTPCTVGIGACARTGIWICNGAGTG
                     LVCSV"
     gene            complement(791..1585)
                     /locus_tag="PLF48_03650"
     CDS             complement(791..1585)
                     /locus_tag="PLF48_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48452.1"
                     /translation="MSDFLLRTRNCEIKYEHYFPNELVDEVQINTEDQQLISAWFISK
                     DTSKAVILAPGKGQNRLAMINKARYYLKRNYSVLMPDLRATGNSEGEYNSLGYYERFD
                     IEACYDFLRKKNFKIIGAQGHSLGAAAIVYTTLEKYNFDFLILESCYDNMQHAFENRV
                     HLFYMPAFLFKPVCWFGEAKIDIGLDKLNPQQLVKNIKTPVLFLYGDSEWQIPIEESE
                     ALYNACGSTNKMKHVFTHGAHEDFSIRYKKEYEQTLDSFLEKYAID"
     gene            1802..3157
                     /locus_tag="PLF48_03655"
     CDS             1802..3157
                     /locus_tag="PLF48_03655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF48453.1"
                     /translation="MKSAFIKEHRLLIIILCVALIIRLLLFIRFEPWNTDMLNRVILI
                     FDSLGYHQLALCIKNEFSFCGNAFRTPAYPFFVSLVYLIFGESPKYVLFLQIFINLGS
                     IMLMYRCGKLLFGMAAGNIAAFLFSIDLHHIVYIYYLLTETLYTFIFLSALYLFLLSI
                     KQNRLVQLCIAGILLGISTLIRPISQYYVLCVAVFTFIYFFMQNKKKAFSYTFILLAC
                     YYVTIAPWCLRNYKLYGAYKVSNIQGYNLLFWNASYYEAKRQHKTIEEINKSFETELQ
                     KRGWRYDSNPFLKEKLDGEMANEILSAHFPDYLKTHLTGTVKIHLSLGTQSLTDVLRL
                     PAKKFTEEEKYTNGVFVLIQKFFKQKTIYEILLGLYVALFLAVVYSFAAIGVWRMIKE
                     KQTLLMLFLLGSAGYFVLISGIISYARYRLPSMPFYILLASCGMVWCLQQIKEKKNKT
                     V"
     gene            complement(3163..4026)
                     /locus_tag="PLF48_03660"
     CDS             complement(3163..4026)
                     /locus_tag="PLF48_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNF48454.1"
                     /translation="MDTVKIIEFPRDAMQGIKEFIPTDQKIEYLNALLKVGFQTIDCG
                     SFVSDKAIPQMADTRIVLSQLKLDETDTLLSVIVGNNRGAEEAGDMDEITYVGYPFSI
                     SETFQTRNTNCTLEESLLRVDEIQNTCAIHGKTFVCYISMAFGNPYGDFYDPALVEHW
                     VDRLLELGITKFSISDTIGAATPELITSVFTTLKEDFPEIGFGAHFHTTPTTWEEKIQ
                     AAWDAGCNRFDGAIKGYGGCPMAKDELTGNMPTEKLLAFFDERNIKHGLDKEAFIEAM
                     SLANKIFGKYA"
     gene            4119..4670
                     /locus_tag="PLF48_03665"
     CDS             4119..4670
                     /locus_tag="PLF48_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF48455.1"
                     /translation="MEPNRLQEIYQKTILFAGEKHSMAGQYLTGTTISYVVHLSNVAM
                     EILIAHQHQPIPDINLAVQVALLHDVLEDTPTDMEELKSIFGDLVANAVLSLTKNKLL
                     PENEQMPDSLNRIKQQGSTVASVKLADRITNLQPPPAQWNINKISAYCDEAVLILTKL
                     VGANTYLENRLSYCIEQYARWIK"
     gene            complement(4690..4911)
                     /locus_tag="PLF48_03670"
     CDS             complement(4690..4911)
                     /locus_tag="PLF48_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNF48456.1"
                     /translation="MRIKGALDKYVKPAVEMDGGNISFVRFEDGLLVLQLQGSCSGCP
                     SSTITLKQGIENLMKRFIPEVREVVAENE"
     gene            complement(5005..5256)
                     /locus_tag="PLF48_03675"
                     /pseudo
     CDS             complement(5005..5256)
                     /locus_tag="PLF48_03675"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
     gene            complement(5292..6047)
                     /locus_tag="PLF48_03680"
     CDS             complement(5292..6047)
                     /locus_tag="PLF48_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF48457.1"
                     /translation="MKTILITGATSGFGEAAAHKFAANGWNLIITGRRKDRLAKTEEE
                     LKSKFGIAVLSLCFDIRNLKEVQNAIHSLPNDWKNIDLLLNNAGLAAGRGPIQDGLYE
                     DWEQMIDTNVKGLLYMIREVSPLMIKRQTGHIINIASLAGWEAYGGGNVYCGTKHAVR
                     AISRSARIDLLQHNIRVSVVSPGAADTEFSLVRFKGDKEKADAVYEGFKPLQAEDVAE
                     SIYFMATQPPHVNIEEIFILPTAQATATIVHKK"
     gene            6122..6448
                     /locus_tag="PLF48_03685"
     CDS             6122..6448
                     /locus_tag="PLF48_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNF48458.1"
                     /translation="MTLEEAQLSIDNWIKEYGVRYFNELTNMAILTEEVGEVARIMAR
                     KYGEQSFKESDMDKNLGDELADVLFVLICIANQTGVNLTEAFEKNLAKKTTRDADRHK
                     QNDKLR"
     gene            6531..8429
                     /locus_tag="PLF48_03690"
     CDS             6531..8429
                     /locus_tag="PLF48_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNF48459.1"
                     /translation="MKRFSLLILCFVFFLYLQAENPKIVSHKISDIILYPSGAQINRE
                     GNVQLQSGTNEIVFKNLSSSIDINSIQVIGNGNYTILSVKHKYNYLEEKNKADEIAEI
                     QSKMDSVQQVLAKENALLTAYIEQQNMLIKNQYIGGSNTGIKAEDLKEVADLHLAKMT
                     EVKLKQVEINAKIDKLQDLKNRLSLQQKEAKSKLTRATSEIWVSLSSATSQKTSLQLT
                     YFESQASWTLFYDLRVSDVNEPLHIDYKAKIKQNTTEDWKDVQLTLSTSEPKSTAQLP
                     YLGTWYLYFNNYLKDYRNLNDAYKNVDISTLPQYSQVSGKIIDNKTGEALIGVNVTVQ
                     GTTVGTVTDIDGFFSLQLPQGEKQVVISYVGYKTITVPVVSEFLNLGLEEDGAALDEV
                     VVSALSIKRKKKYAGSPGASNKIIYETDAVDMEEMLTPIDVNFVIKNPYTINGDNKEN
                     IVEIKSETVKPIYKYYAIPKLDKSVFLTANITNWENLNLLAGEANLYFQNNYVGRTLV
                     NPAITEDTLMLSLGRDKNIVINREKVKEFSKKQFLSGDKVINNSFSISIRNNKKQPID
                     LTIYDQIPVSTTKEIKVDLLEKQEADLDETTGKLTWKIFVEPTKEKKLQFGYAVKYPK
                     NKILYLKE"
     gene            complement(8674..10026)
                     /locus_tag="PLF48_03695"
     CDS             complement(8674..10026)
                     /locus_tag="PLF48_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNF48460.1"
                     /translation="MKIRVVKTASQANAVHVVQYQYGKRRVMQHFGSAHTETALNDLL
                     ILAEEWIKNYSHQLSIFADENPNQILHLNHSTFLGVKYRFFHQQIAMIQTAIGLDDLP
                     SVLNDLVAVRIFEPASKLRSLDLMEQYFGIRHSRKAFYKFAPKCIELKDLVEGKILSF
                     AKAHYAFSYDLLFYDVTTLYFETFEQDELRKNGFSKDNKSQQPQILVALMVSKEGFPV
                     TYEIFSGNTFEGKTIVPVINDFIKRNGVENFTVVADAAMISTANVNLLAQNKINYIVG
                     ARLGNVSAELLQDINKKLRREDGKNIRIKTESGDLICTYSAVRYRKDKYEMEKQIEKA
                     KQVVEKPSKSKKLKFTQTDGQQISLNEALIEKTTKLLGIKGYYTNLDEAIANNAIIIE
                     RYHELYRVEQAFRISKNDLQTRPIFHFKEEPIKLHLLICFMGISDSKTHRTENSNIHP
                     "
     gene            10601..11221
                     /locus_tag="PLF48_03700"
     CDS             10601..11221
                     /locus_tag="PLF48_03700"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HNF48461.1"
                     /translation="MAKVYFICIFLLSGFSDSCKSTSATLADNPKAEVKLSNKQLALI
                     NNLNRQAQITTSYNPAYVAIPFPNGDVDSTTGVCADVIVRALRAIDIDLQKEIHTDIS
                     KHFNSYPNIWHSTQPDKNIDHRRVPNIMCYLQLKGKAVVLSTVNEDYKAGDIVAWKLD
                     NGLYHIGMVSDKVSESNTPLIFHNIGSGTKEADVLFWWKIIGHYRI"
     gene            complement(11879..>12549)
                     /locus_tag="PLF48_03705"
     CDS             complement(11879..>12549)
                     /locus_tag="PLF48_03705"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNF48462.1"
                     /translation="EDLSDYPETDGVVINYKYFGTTGTSTSPYNLGRTATHEIGHWLG
                     LEHIWGDDADCSGTDNIDDTPNQQTETYGCPSGEKTDNCTTNSPGIMYQNYMDYTDDA
                     CMNMFTLQQASRMRGILQTIRSSIATSNKCSTTTAVLQSNSPDFSVYPNPVNRYVRFS
                     GLPNSNEKFELELFAIDGKLLYTTSINNTLDMIELPELENGNYLVKLSNSRSVYVAKI
                     LVRK"
BASE COUNT         3934 a         2289 c         2314 g         4012 t
ORIGIN      
        1 taacagagca aacaagccca gttcctgcgc cattacagat ccatataccg gttcgcgcac
       61 aagcacctat cccaactgta catggagtcc cttttaaggg aaatgtttca tcaatacttc
      121 cgttgcaatt atcatctaaa ttattgcaaa gctctgtatt ccccggaaat attgaattat
      181 tatcatcatt gcaatcagtt ttattagaaa cataacaagg aaaagtcgta ccacaagcca
      241 gtaagggttc gcatgcatca ccaaaaccat ctccatcatt atctttgtag agatacgtaa
      301 agatattgca cgatggcgtt gctatccagt tcgctccatt ccaaactaaa atatcgccgg
      361 cattattgcc cataataaag ttattgaaat tgtaggtcgg cgttatacgt tcccatttag
      421 ttaatgcaag gttatacata aacaaagcac tactatccgt atcataaacg attaacgcat
      481 ttgcaggtga tgcgattgaa ttgcgctgta ccgcagtcat tctgggtaaa agaaatcctt
      541 tctgtgtgct ggatacatct aacatggcac tgctattcgg tgcagcaccg gtggagttta
      601 tgcctatgtt ttgtgctata gcaacatgca atagcatcag caatactgaa ataaaggtca
      661 gttttttcat acttagtttt tgatgtataa atttatgaaa ataaattatt cctgcaaacc
      721 tttattaatc ataagcaata cacattaagc atattccata ttgaaaaaat catgtggtca
      781 tagtaattat ttaatcaata gcatattttt ccagaaagct gtctagtgtt tgttcatatt
      841 cctttttata cctaatgctg aaatcctcgt gagctccatg tgtaaaaacg tgtttcattt
      901 tattagttga accacatgca ttatataggg cttcgctctc ttctattggg atttgccatt
      961 ctgaatcgcc gtataaaaac aaaacaggcg ttttaatgtt ttttactaat tgctgtgggt
     1021 tcagcttatc caaaccgata tctattttgg cttcaccaaa ccaacaaaca ggcttaaata
     1081 aaaatgccgg catataaaaa aggtgtacgc gattttcgaa agcatgctgc atattatcat
     1141 agcaagattc cagtatcagg aaatcaaaat tgtatttttc cagtgtggta tatacaattg
     1201 cagcagctcc tagcgaatgg ccttgtgcac cgattatctt aaaattcttc tttctcagaa
     1261 aatcatagca agcttcaata tcaaaacgtt catagtagcc caatgaatta tactcacctt
     1321 cactatttcc tgtagctctg aggtcgggca ttaatacaga atagtttcgc tttagataat
     1381 agcgtgcttt attaatcatg gctagtctgt tttgtccttt gcccggtgct aaaataaccg
     1441 cttttgatgt atccttactt ataaaccatg ctgaaataag ctgttgatct tcggtgttga
     1501 tttgtacttc gtcaacaagt tcattaggaa aatagtgctc gtattttatt tcacaatttc
     1561 ttgtacgcaa taaaaaatct gacataagtt tacaagacaa caagtagagt aaaaataaaa
     1621 tgctgattag tttaatcttg tttctgtatt tatacatgct gcaaaatgac gaatcttatt
     1681 tgtaaagatt tgtcaaaata atttaagaat tcttatttac gttaaaaaaa taaaaatagt
     1741 gcatatttta atgcagtatg attctgcctc atcatgcact aaaaattttt atctttatag
     1801 cgtgaagtct gcttttatca aagaacatag gttgcttatc attatattgt gtgtagcctt
     1861 aataatccgg ctattgttat ttattcgttt tgagccatgg aatacagata tgctgaatcg
     1921 tgtcattctc atttttgatt ccttgggcta tcaccagctg gcattatgta taaaaaatga
     1981 attcagtttt tgtggcaatg ctttccgtac acctgcctat cctttcttcg tgagtttggt
     2041 ttatttaatt tttggcgagt cgccaaagta tgttcttttt cttcaaatat ttatcaattt
     2101 aggttcgata atgctgatgt atagatgtgg caaattatta tttgggatgg ctgccggaaa
     2161 tattgctgct tttttgtttt ctatcgattt gcatcatatc gtgtatatct actatctgct
     2221 tacagaaaca ctgtatacct ttatcttttt atctgcgctc tatctgtttt tactcagcat
     2281 aaaacaaaac cgactggtgc aactgtgcat agcgggtatc ttgttaggta tctccacact
     2341 gatacgaccc atctctcaat attatgtgct ttgtgtggct gtttttactt tcatttattt
     2401 ctttatgcaa aacaagaaaa aggcatttag ttatactttt attttgctcg catgttatta
     2461 tgttactatc gcaccttggt gtttgcgaaa ttataaactg tacggtgctt ataaagtatc
     2521 taatatacaa gggtataatt tattattctg gaatgccagt tattatgaag caaagcgtca
     2581 acataaaacc attgaagaaa tcaataaatc atttgaaact gaactgcaaa aaagaggatg
     2641 gaggtacgac agtaatccct ttttgaaaga aaaactggac ggcgaaatgg caaatgaaat
     2701 tttatctgct cattttccgg actacctcaa aacgcattta accggaacag taaaaattca
     2761 tttgagttta ggtacacaga gcctgactga tgtattacgg ctgcccgcta aaaaatttac
     2821 agaagaagaa aaatatacca atggtgtatt cgtactgata caaaagtttt tcaaacaaaa
     2881 aactatatat gagatactcc tgggattata tgttgctttg tttctggcag tagtctacag
     2941 ctttgccgct attggtgtat ggcgtatgat aaaagaaaaa caaaccttat tgatgttgtt
     3001 tttattgggc tcagccggat attttgtact gataagtggt attatttcgt acgcccgcta
     3061 ccgattgccg agtatgccat tttatatctt gctggcaagt tgcggtatgg tttggtgtct
     3121 gcaacaaatc aaagaaaaga agaataaaac ggtgtagtct tattaagcgt atttgccgaa
     3181 aattttattg gcaagactca tggcttcaat aaaagcttct ttgtctaatc cgtgtttaat
     3241 attgcgttcg tcaaaaaatg ccagtaattt ttcggtgggc atattgcccg taagctcgtc
     3301 cttagccata gggcatccgc cataaccttt tatagcaccg tcaaatctgt tgcagcctgc
     3361 atcccatgca gcttgtattt tttcttccca ggttgtaggt gtggtatgaa aatgcgcacc
     3421 aaatcctatt tccggaaaat cttcttttaa tgtggtgaat acacttgtaa ttaattccgg
     3481 tgttgctgcg cctattgtat ctgaaataga gaatttagta atgcctaatt ccagcaacct
     3541 gtccacccaa tgttctacca atgccggatc ataaaaatca ccgtatggat ttccaaacgc
     3601 catggagata tagcaaacaa atgttttgcc atgtatggca caggtatttt gaatttcatc
     3661 tacacgcaat aatgactcct ctagggtgca attggtattg cgcgtctgaa atgtttcgga
     3721 aattgaaaac ggatagccaa cgtaagtaat ttcatccata tcgccggctt cttccgcacc
     3781 gcggttattt cctacgataa cagacaataa agtatctgtt tcgtccagct ttagctggga
     3841 aagcacaata cgtgtatccg ccatttgcgg tatggcttta tctgatacaa agctgccaca
     3901 atctatcgtc tgaaagccca cttttaataa ggcattcagg tattcaatct tttgatctgt
     3961 aggtataaat tctttgatac cctgcatggc atcgcgcgga aattcaatga tttttactgt
     4021 atccatgttg caaaaataag gtattctgct taagataaga aaggatgact aacacgcacg
     4081 tcaattttgt aaaagataaa acgtattttt agaacaagat ggaaccgaac cgcttacagg
     4141 aaatttacca aaaaacgatt ttatttgccg gggaaaaaca ttcgatggct ggtcagtatt
     4201 taacaggcac aactatttct tatgttgtcc atttaagtaa tgtggctatg gaaatactaa
     4261 tagcacatca gcatcagcct ataccggata ttaatcttgc cgttcaggtg gcattgttgc
     4321 atgatgtgtt agaagacaca ccaaccgata tggaagaatt gaaatcaata ttcggtgatt
     4381 tagtggccaa tgccgtactt tctctgacaa agaataaact attacctgaa aacgaacaaa
     4441 tgccggattc gctaaacagg ataaagcaac aagggtctac ggtagccagt gtaaaattag
     4501 ctgacagaat taccaatctg caaccaccac ctgctcaatg gaatataaat aagattagtg
     4561 cttactgcga tgaagcagtg ctcattctca caaaattagt cggagcaaat acttatctag
     4621 agaacagact ttcatattgc atagaacaat atgcaagatg gataaaataa gataaataag
     4681 cggttttgat tattcattct ctgccactac ttcccgaact tcaggtataa agcgtttcat
     4741 caggttttcg atgccttgtt tcaaagtaat ggttgatgat ggacagccgc tgcaagaacc
     4801 ttgaagctgt aataccagta agccgtcttc aaaacgaaca aaggagatat tgccaccgtc
     4861 catttccaca gccggcttca cgtatttatc caatgctcct ttgatgcgga ttactacatc
     4921 ttcatcgtca tctgctacag catttgatgc cgtgcgttca ggctttatga attgctctgt
     4981 gaacaagggt ttcccggttg aaatatattg tttgataaaa tcctttaaag gtatcatcaa
     5041 ttcagcccac aatatttcat cttctttgtt gatagaaata aagttattac tgataaaaac
     5101 accctttact ccgttaaagg caaaaagttc gtgtgccagc ggagcttctt gtgtttcttc
     5161 agctgaaaaa aaatctacgc tgtcatttaa gaataggact ttattggtta caaatttcaa
     5221 ggtattcgga ttcggtgtgg cttccgaata tatcatgacg ggtatttgag ttgtttgttc
     5281 ttccataact attatttttt atgaacaatt gttgccgtgg cttgtgccgt cggcagtata
     5341 aaaatttctt ctatattaac atgaggtggc tgggtggcca taaaataaat gctctctgcc
     5401 acatcttcag cttgcaatgg cttgaaccct tcgtaaacag catccgcttt ttctttatca
     5461 cccttaaatc ttaccagact aaattcagta tcggccgccc ctggacttac aacagatact
     5521 cgtatattat gctgtaataa atcgatgcgg gcactgcgag aaattgcacg cactgcatgt
     5581 ttggtaccgc aatagacatt accaccgcca taagcttccc agcctgccag cgaggcgata
     5641 ttgatgatat gtcctgtttg acgttttatc ataagtggtg aaacctctcg aatcatatag
     5701 agcaaacctt tcacgttagt atctatcatt tgttcccaat cttcatataa tccatcctga
     5761 attggacctc tgccggcagc cagcccggca ttattgagca ataaatcaat atttttccag
     5821 tcattaggca aagaatgaat agcattctgt acttctttta gatttctgat atcaaaacat
     5881 aagctcagta ccgctatgcc aaatttggac tttagctctt cttctgtctt tgccagtctg
     5941 tctttccttc ttcccgtaat aatcagattc cagccgtttg cagcaaattt atgtgctgcc
     6001 gcttctccaa aacctgatgt cgctcccgtg attaatattg ttttcattat tattgctaaa
     6061 ccaagaaaaa ttcaggataa aaatagcgaa ataactttta tttttagaaa aagctgaaag
     6121 tatgacctta gaagaagcac aactatctat tgataattgg ataaaagaat atggtgttcg
     6181 ctatttcaac gagttgacca atatggcaat tttaacggaa gaagtaggtg aagtagccag
     6241 gataatggct agaaaatacg gagagcaatc tttcaaagaa tcggatatgg ataaaaactt
     6301 gggcgatgaa ctggcagatg tgctttttgt gctgatttgc attgcgaacc aaaccggagt
     6361 aaatctgaca gaagctttcg aaaagaattt agccaaaaaa actaccagag atgctgacag
     6421 gcataagcag aatgataaac tgagatgaga tgggttgctg aatttagtaa ttaatataaa
     6481 attcacaccc gatcagggat atttatataa attacactaa attctaaaat atgaaaagat
     6541 tcagtttact catactttgc tttgtttttt ttctctattt gcaggcagaa aatccaaaga
     6601 ttgtctcaca taaaatttca gatatcattt tgtatccttc cggtgcacaa atcaacagag
     6661 agggcaatgt tcaattacaa tccggcacta atgaaattgt gtttaaaaat ttatcgtcaa
     6721 gtatagatat aaacagcatt caagtgatag gcaatggcaa ttatactatt ttatccgtaa
     6781 agcataaata taactatctg gaagagaaaa ataaggcaga tgaaatcgct gaaatccagt
     6841 ctaagatgga ttcagttcag caagtattag ctaaggaaaa tgcacttctt actgcatata
     6901 tagaacaaca gaatatgtta ataaagaatc aatatattgg aggcagcaat accggcataa
     6961 aggcagagga tctaaaagaa gtcgctgatt tacatctggc taaaatgaca gaagtaaaac
     7021 taaaacaagt tgaaataaat gccaaaattg ataaattaca agatcttaaa aacaggttat
     7081 ctttacagca aaaagaagca aaaagcaaac tgaccagagc aaccagtgag atttgggtta
     7141 gcctttcttc agccacttca caaaagacca gccttcagtt aacgtatttt gagagtcagg
     7201 catcctggac actcttttat gatttaagag ttagtgatgt aaacgagcca ttacatattg
     7261 actataaagc taaaataaaa caaaacacaa cagaagattg gaaggatgta caacttactt
     7321 tgtcaacctc tgaaccaaaa tcaactgctc aattgcctta tctgggaact tggtatttat
     7381 actttaataa ctatctgaaa gactatagaa acttaaatga tgcgtacaaa aatgtggaca
     7441 tcagtacttt accacaatat agccaagtga gtggtaaaat aattgataat aagacaggag
     7501 aagcactaat tggtgtaaat gtaacggtac aaggcaccac ggtaggtact gtaacggata
     7561 tagatggctt ttttagttta caattacctc agggggaaaa acaggtagta attagttatg
     7621 tgggttacaa aacaattact gttcctgttg tctctgaatt tctaaatctc ggcttagaag
     7681 aagacggagc tgctttagat gaagtagttg taagtgcttt gagtattaaa cgcaaaaaga
     7741 aatatgcagg ttcacccgga gcatcaaata aaatcatcta tgaaacggat gccgttgata
     7801 tggaggaaat gctaactccg attgatgtca attttgttat taaaaaccca tacacgatta
     7861 atggtgacaa taaggagaat atcgttgaaa taaaatcaga aacagtaaaa ccgatttata
     7921 aatattacgc aattccaaaa ttagataaga gtgtatttct tactgcaaat ataactaatt
     7981 gggaaaatct gaatttgtta gccggagagg caaacttgta tttccaaaac aattatgtcg
     8041 gacgtactct agtcaaccct gccattactg aagatacact tatgctctca ttaggtcgcg
     8101 ataaaaatat tgtgattaac agagagaagg taaaagaatt cagtaagaaa caatttttat
     8161 caggggataa ggtcatcaat aattcgttct caatttctat tcgcaacaac aagaaacaac
     8221 ctatagattt gaccatctat gatcagattc ccgtttcaac tactaaagaa ataaaagtag
     8281 acttactgga aaagcaggaa gctgatttag atgagactac cggaaaactg acatggaaga
     8341 tctttgttga acctacaaaa gagaagaaac ttcaatttgg atatgctgtt aaatacccta
     8401 aaaacaagat actttatctt aaagaataat tcatttggcg gtttaaaacc catagaatta
     8461 tagccgttta atcttaaata cctgagttcg ggataagcgt ttcattaaaa aaatagttga
     8521 tttttcctga cttgtcccat ttagtgcggt gggtttaatt tttctattag gtgtttcatt
     8581 ttgggagttg gttgtgcctt tacggtaatt gttttgttgg ttatgaggtt tagtatttct
     8641 ccgtcaacta tttttttcga ttcgtctata aatctacgga tggatattgc tgttttcagt
     8701 tcgatgtgtt ttgctatcac taatgcccat aaagcaaatc agtaggtgta atttgatagg
     8761 ttcttcttta aaatggaaaa taggacgggt ctgtaagtcg tttttggata ttctgaaagc
     8821 ctgttctacc ctgtacagtt cgtggtagcg ttctatgata attgcattgt ttgcaatggc
     8881 ttcgtcaaga ttggtgtaat acccttttat gccgagtagc ttggtggtct tttcaatcaa
     8941 agcttcgttg agcgatattt gttgcccgtc agtttgggta aattttagtt ttttgctttt
     9001 tgagggtttt tccaccacct gcttcgcctt ttctatctgc ttttccattt cgtatttatc
     9061 tttacggtat cggacggctg aataagtgca aatcagatct ccgctttctg ttttgatgcg
     9121 gatgtttttg ccgtcttccc tcctcaattt tttattgatg tcttgcaaca attctgccga
     9181 cacattgccc aatcttgctc ccacaatgta gttaatcttg ttttgtgcca gcaggttcac
     9241 atttgctgtg ctaatcatgg cggcatctgc cactacagta aagttttcta caccatttct
     9301 tttaatgaaa tcattaatga cagggacaat cgtttttccc tcaaacgtgt taccggaaaa
     9361 gatttcataa gttaccggaa aaccttcttt gcttaccatc agtgctacca aaatttgggg
     9421 ttgctgggat ttgttgtctt tcgaaaaccc gtttttccgt agttcgtctt gctcaaatgt
     9481 ctcaaaataa agcgtggtta catcataaaa caataggtcg taactgaagg cgtaatgcgc
     9541 cttggcaaag gacaatattt taccttctac caagtctttt agttcaatac attttggagc
     9601 aaatttgtaa aacgctttac ggctatggcg gatgccgaaa tactgctcca tcagatccag
     9661 agaacgtagt ttggaggcgg gttcaaagat tctcaccgct accaaatcat tcaatactga
     9721 aggcaggtca tccaagccaa tggcggtttg tatcattgca atttgctgat gaaaaaagcg
     9781 gtatttgact cccaaaaagg tactgtggtt gaggtgtaaa atttggttgg ggttttcgtc
     9841 agcaaaaata gaaagttggt ggctgtaatt tttaatccat tcctcagcca atatcagcaa
     9901 atcatttagt gccgtttccg tatgggcaga gccaaagtgc tgcatcaccc ttcttttgcc
     9961 gtattgatac tgtacaacat gcacggcatt tgcctgtgag gcggtcttga ctaccctgat
    10021 tttcatatcc aaatatacct cttagtgcgg tatttttagt gctttaaaat accatacagt
    10081 tgattgtcaa tagattatga ccgcactaaa tttactgggg acaagtcagg acaagacact
    10141 ttatattaaa gaataattca tttggcggtt taaaacccat agaattatag ccgtttaaat
    10201 cttaaatacc tgagttcggg ataagcgttt cattaaaaaa atagttgatt ttttatatag
    10261 aaccacctcc ttttaattcc tcctccttgt taagttggag gaaaaaatga gaaatcaact
    10321 atttttttca ccttaatttt cttaacccga actcaggtta aatagttgtt tggtataaat
    10381 ttagcttaga ttgtctatca aaaacatagt atcgccaaag acgattccta tatttttcta
    10441 ttctcatttc atttttttat tgtgaggacg ttattttaaa aaaatctatg catctatgtg
    10501 gtaaatatta taaatcaagc tgcacaaggg ggcatagcat aatttacatt tattaaccaa
    10561 aagaatagac gattcatatc cgatttgcgt ttgttcttgc atggcaaaag tgtattttat
    10621 ttgtattttc ttgctttcag gattctcaga ctcctgcaaa tcaacatctg caactcttgc
    10681 cgataatcct aaagcagaag tcaagctcag caataaacaa cttgccctga taaacaacct
    10741 taacagacaa gcacaaatta ctacttcata caatccggct tatgtggcaa taccctttcc
    10801 aaacggagac gttgacagca caacaggcgt ttgtgctgat gtaatcgtac gtgcccttcg
    10861 ggctatagat attgatttgc aaaaagaaat acacacagac atcagtaaac attttaatag
    10921 ttacccaaat atttggcatt ctacccagcc cgacaaaaat attgaccatc gaagagtacc
    10981 caatattatg tgctacttgc aattgaaagg aaaggcggtt gtgctctcca ccgtaaatga
    11041 agattacaaa gccggagata ttgtagcatg gaagttagac aatggtctct atcatatcgg
    11101 catggtcagt gataaggtta gtgaatcaaa tactcctttg atttttcata atatcggtag
    11161 cggaacaaaa gaagctgatg tcttattctg gtggaaaata attggccatt acaggatata
    11221 agtcatacca gaaagtagat tatccttgtt cagtattaga agaaaataag tgaatagctc
    11281 ttgataaaag agcaacattt ttttgctaag atctgaaatt aacagcatct ggttttttta
    11341 ttatattatg ttcaaaataa ttcggtaaag attaataaaa ataacaaaat gaaacccgtt
    11401 atgtggttta aaacaaacta ctggaaaata aaataatgag agttgcattg gcagcgtgta
    11461 aagaagcgtg ggttggcaaa acgcgtagct aagcgtaggt atgcgcgtag aacgatcgtt
    11521 ttgccttgtc ttttttggtt actttttttg actaagaaaa aaagtgacag agtttaatat
    11581 taataatcaa tggaattaga aagatgaatt actaattcaa accatacaac aggttaaatg
    11641 aaatatatct aaccagaccg aaaaaagagt taggtttgtg aaagatacag ttcctattat
    11701 ttgagaatag aagtaaaatc aaaaatcagc agggctaaag ttgttgccaa tcggctgata
    11761 aaaattgcct tgtctttcac aaatgctttt tttagttgca tttttgtata agttcgtaat
    11821 atataatttc aatttaatta cattttttaa gtaaaatgca tgtcccaaac acaggatgtt
    11881 atttgcgcac caatattttg gccacataaa ccgaacggct attgctgagt tttactagat
    11941 agtttccatt ttcaagctca ggcaactcaa tcatgtctaa agtattattg atgcttgttg
    12001 tataaagcaa tttcccatca atggcaaata actcaagttc aaacttttca tttgaattcg
    12061 gcaatccgga aaatcggaca tagcggttca ccggattggg ataaacagaa aagtcaggcg
    12121 aatttgattg taaaactgcc gttgtagtag agcatttgtt ggaagttgcg atactggaac
    12181 gaatagtttg taagatgcct cgcatccggc ttgcctgctg taaggtaaac atattcatac
    12241 acgcatcatc agtatagtcc atataattct gatacataat gcccggcgaa tttgtcgtgc
    12301 aattatccgt tttttctcct gacggacagc cataagtttc tgtctgctga ttgggggtgt
    12361 catcaatatt gtccgttccc gaacagtctg catcatctcc ccagatatgt tccagcccca
    12421 gccagtgccc gatttcgtga gtggctgtcc tgcccaaatt atatggggaa gtggaagtgc
    12481 cggtagtgcc gaaatattta taattaatta cgactccgtc agtttcagga tagtcagaca
    12541 gatcttcag
//
LOCUS       DAOQII010000048        12107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2623_length_12107_cov_4.782028, whole genome shotgun sequence.
ACCESSION   DAOQII010000048 DAOQII010000000
VERSION     DAOQII010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_12107_cov_4.782028"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLF48_03710"
     CDS             complement(<1..776)
                     /locus_tag="PLF48_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48463.1"
                     /translation="MNTHIPTKEQLAGFEKELDALRAVTISQLGEYDEKHIKRIILVQ
                     RYAEISGRALLQFGLFPPFWLAGTALLSVSKILDNMEIGHNVMHGQYDWMNDPTIHSS
                     NFEWDTACAAKSWRRTHNFEHHTFTNIIGKDRDYGYGILRMDNTSPFQKDDYFNLLKF
                     ALLSAFFQYGVALHELESDRIKKGEIQLKDKRNFLKDLAKKGGRQIVKDYVFFPALGA
                     LTGSTLPVLAGNAVANLVRNLWASTVIFCGHFPEGSHTFD"
     gene            complement(797..1873)
                     /locus_tag="PLF48_03715"
     CDS             complement(797..1873)
                     /locus_tag="PLF48_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HNF48464.1"
                     /translation="MAAPIFNEIRTQLNNMLFNTKASYYFEPLVEKYFPSKSLVSTKA
                     TVVSVNKENENMLTIKLKPNRKWNGFIAGQYVETQLKINAVNYTRIFSISSSEDEYKS
                     SGTITLTIQKQDQGKVTPWLFENLEVGSIIKISDAKGEFILHDKNRPVLMIAGGSGIT
                     PFYAMLQKCANENTNAVLLYYAKDKQHIFRNKLDKLTLNKQITVHYMSSTTEGRLSKQ
                     QLEKYCPDYYFRQVFICGPNTMISDTTMLLKHNSVDADNIFSEYFKPVLYPVHFKFNK
                     NKSKIIYKNKEIYTAGSKTILEQLEAHGENPKHGCRMGLCKECQCTKNKGIVYNTLTQ
                     KFSEATPETIQICVSIPVGDVEII"
     gene            complement(1973..2572)
                     /locus_tag="PLF48_03720"
     CDS             complement(1973..2572)
                     /locus_tag="PLF48_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF48465.1"
                     /translation="MDNFANDQFRIYSDGIKKSFPDLSYAKIMELIPHFEFQQISKRS
                     EIIKTGQFYGKIVFVIDGLFRAYYKYNDIENTFWFREEFTVFASHRSILQNKPSTISY
                     QALEDSIIAVIDYSVLKELAKNDAEVGKSIMIVLESLILELIDRVEEFITLNPEQRYL
                     YFLEKHDKVINRVPQQQLASYIGITPESFSRLKSRMMSK"
     gene            complement(2728..3564)
                     /gene="lgt"
                     /locus_tag="PLF48_03725"
     CDS             complement(2728..3564)
                     /gene="lgt"
                     /locus_tag="PLF48_03725"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNF48466.1"
                     /translation="MINFLSFVIHWNANPVIIQITDSYGIRWYSLLYGLAFLLGYYIL
                     QWEFKKENKNPEGAEELLMYMFFAVLIGARLGHCFFYDWDYYKDHLLEILQVWKGGLA
                     SHGAAIAIPIALYLYKRRHPDQSYLWIIDRVSIPTALGASFVRYGNFFNHEIVGIPTN
                     NPNWGVIFMQCTEDNGIPTPRYPAQLFEAFLYDFIFIVLLIMYKRANYKPREGTLMSV
                     FLFIMFIGRFFIESVKYDRNPFHFGSFTFTHGQLLSIPFIIAGFFIFYLSRKQPKQDT
                     PA"
     gene            3723..4343
                     /locus_tag="PLF48_03730"
     CDS             3723..4343
                     /locus_tag="PLF48_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF48467.1"
                     /translation="MNIADADDLVYVQELCNLYEESAKKRGTGIAKRDPKYLELKILE
                     SKAIIAKTNKGELAGFCYIETWEGKNYVANSGLIVKENFRHHGLAKRIKKFVFEYTQK
                     KYPNAKIFGITTSLAVMKLNSDLGYRPVTFSELTQDDAFWNGCQSCINFDILSRTHRK
                     NCLCTGMLFDPKEDKKKVFAKAQKVERKIPAKLKTPKAQRIKAVKK"
     gene            4542..7007
                     /gene="thrA"
                     /locus_tag="PLF48_03735"
     CDS             4542..7007
                     /gene="thrA"
                     /locus_tag="PLF48_03735"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNF48468.1"
                     /translation="MKILKFGGTSVGSVQTIRMIIDIIRKEKENHEDLVLVCSAFSKV
                     TDQLINAGKLAEKKDNTTYLEIFESFKERHRLALEELVSNNEIKSVMLEEFFDSMENV
                     KDILKGIHLLGELSEKTLAQLVSYGERFSCFIVAGALRNAGLEGIYVNATKLVRTDSN
                     YLSAKVNFDKTNKNILDFFEAIHGTAVITGFIGANEHGTVTTLGRGGSDYTAAIFGAA
                     LKADVIEIWTDVDGVLTADPRRVDQAFTVPMLSYKEAMELSHFGAKVIYPPTIQPAYI
                     KNIPIIIKNTFNPEHPGTFISKESGKSNNAIKGISSISDIAVLRMEGTGMVGVVGIAS
                     RLFSALSRAQINIIFLTQGSSEHSICFGILPNEIKKAHKAVEEEFRYEIQSKHIAPLV
                     VEINNSIIAVIGENMSNVPGVSAKMFKALGNNGINVKAIAQGSSELNITAVIDKHNEA
                     KALNALHEIFFSTDKHSINVFLVGPTGLIGKTLLNQIRKQFDFLKNEKNILINVTGII
                     NSKKMLIDSNGIDLSDWENELNENGDESNLAHFTEKLIDLNFANSVLIDCSASKQVVE
                     YYEKLLKKSINIVTPNKIANTLSQEKYNRLRELAKRANVHFMYETNVGAGLPVIGVLA
                     SLMNSGDEILKIEAVLSGTLSFIFNTYKDNLKFSDVVLTAKEKGFTEPDPRDDLSGLD
                     VARKALILSRDSGLQMELDDIKVKNLVPENLRNVSVAEFMDRLPELNDEYEKLKKYAE
                     ANQAALRYMAVIENGKVEIALKEVDINHPFYNLSGSDNMIVFTTERYKNNPLVIKGPG
                     AGAEVTAAGVFAEVITIGNYLAN"
     gene            7273..8229
                     /locus_tag="PLF48_03740"
     CDS             7273..8229
                     /locus_tag="PLF48_03740"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNF48469.1"
                     /translation="MTAKSPKRIRVFAPATVANVACGFDVLGFAIDKPGDELIMEITP
                     EKGVQIVDIEGDDGVLPRDAKKNSATVAIQDYLDFIDADFGCKIWLKKMMPSGSGLGS
                     SAASAVAGVFAINMLCDEQLTKEEMLPFLLHAEKAACDAAIADNVAASLFGGFILVRS
                     YEPLDILQIPVPEDLWCAVINPHVIVLTKEAREILPKEIPLAHSLRQSANVGGLMVGL
                     LRGDYDLIGRSLIDYIAEPYRSKLIPGFYDMKNAAIEAGALGSSISGSGPSVFALCHG
                     KEAAQKVGDAMKNVMDKLNIGADVYVSKVNNHGPKVLPLSND"
     gene            8330..8905
                     /locus_tag="PLF48_03745"
     CDS             8330..8905
                     /locus_tag="PLF48_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNF48470.1"
                     /translation="MLGLKLATDPRWVNLAEKSIEEILTDHAYCEQKATSACISLIQL
                     FPDKTYMVDKLVPVVQEEWMHFKLVLDELKKRNLKLGQQRKDEYVNALLKFITNSREK
                     ILLDRLLMSGLIEARSCERFRLLSLEISDDSLKQFYHNLMRAEALHYRMFLDIAEQYY
                     PKDKVRKRWEEWLSFETKVLKAIELRGDRMH"
     gene            complement(9057..9773)
                     /gene="aqpZ"
                     /locus_tag="PLF48_03750"
     CDS             complement(9057..9773)
                     /gene="aqpZ"
                     /locus_tag="PLF48_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNF48471.1"
                     /translation="MEIKSYSKYLAEMLGTMVLVLMGCGSAVIAGADGTTGVGLLGIA
                     FAFGLSVVAMAYAIGHVSGCHINPAISISMLVAGKLSVTDTIGYVISQCIGAILGTGI
                     LYLIVSNAIGGYEMKEWALGANGWGEGYLGQYNSTAAFIAELVFTFIFLLTIFGATST
                     KNINGGFAGVAIGLCLVLIHIVGIKVTGVSVNPARSLGPALFAGGNALSQVWLFWVAP
                     ILGGILAALVHKSVLERNYN"
     gene            complement(9955..10890)
                     /gene="mdh"
                     /locus_tag="PLF48_03755"
     CDS             complement(9955..10890)
                     /gene="mdh"
                     /locus_tag="PLF48_03755"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNF48472.1"
                     /translation="MKVTVVGAGAVGATVADNIARKELCSELVLLDIKEGLAEGKAMD
                     MMQTSAILGFDTKVVGSTNDYSKTANSDVVVITSGIPRKPGMTREELIGTNAGIVKGV
                     CENILKYSPNTIFVIISNPMDTMTYLALKATGLPKNRVIGMGGALDSSRFKYYLSQAL
                     NCSLNDLNAVVIGGHGDTTMIPLTRLANLNSVPVTQLLDKETLDKVAADTMVGGATLT
                     KLLGTSAWYAPGAAGMSVVEAILRDEKRVITSCVYLDGEYGQKDICLGVPVKIGKNGW
                     EEIIDYKLTEEEQAAFNKSADAVRAMNDVLSTLSL"
     gene            11041..>12107
                     /gene="hemN"
                     /locus_tag="PLF48_03760"
     CDS             11041..>12107
                     /gene="hemN"
                     /locus_tag="PLF48_03760"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNF48473.1"
                     /translation="MTDFSIIQKYNVPIPRYTSYPTVPYWQDNKSSNEEWKACMLKAI
                     NNGRTKNGISIYIHLPFCEQLCTYCGCNKRITKNHSVEGVYLEAVLKEWEMYKAVLGT
                     DFTIQEIHLGGGTPTFFSPENLKLLIQNITKDIRIAPDHEFSFEGHPNNTTYAHLKTL
                     FDVGFNRVSFGVQDLNEKVQVAINRVQPFEKTKQVTDWAREIGYTSVNFDLIYGLPFQ
                     TLDSIKDTISRVIGLKPDRLAFYSYAHVPWTKKSQRAYDENDLPKGKDKFALYTAGRE
                     LFLAAGYEDVGMDHFALKDDGMLQCKREGKLHRNFMGYTTNNTEILIGLGVSAISDVY
                     YGYRQNYKTVEEYYEAVNKEE"
BASE COUNT         3938 a         2142 c         2288 g         3739 t
ORIGIN      
        1 tcatcaaagg tatgcgagcc ctcgggaaaa tgaccacaaa aaataaccgt ggaagcccac
       61 aagtttctta ctaaattggc cacggcattc ccggctaaaa cgggaagagt tgacccggtt
      121 aaggctccga gtgccgggaa gaaaacataa tctttaacta tttgccgacc accttttttt
      181 gccaaatctt taagaaaatt tcgtttgtct tttagttgaa tttcgccttt tttaattctg
      241 tcagattcca gctcgtgtaa ggctacgcca tactgaaaaa aggcagatag caaggcaaat
      301 ttcagtaaat tgaaatagtc atctttttga aatggagacg tattatccat acgcaaaatg
      361 ccatatccgt aatcacggtc tttgcctata atattcgtga atgtatggtg ttcaaaatta
      421 tgtgttctgc gccatgattt ggcagcacat gccgtatccc attcaaagtt ggaggagtgg
      481 atagtcgggt cattcatcca gtcgtattgt ccatgcatga cgttatgccc aatttccata
      541 ttgtctaata tttttgatac agacaataaa gcagttccgg ctaaccaaaa tggtggaaaa
      601 agtccgaact gcaataaggc tctgccgctt atttcagcat agcgttgcac taaaataatt
      661 cgcttgatat gtttttcatc atactcgccc aattgtgaaa ttgtaacagc tctaagtgca
      721 tccagctctt tctcaaatcc ggctaattgt tctttagttg gtatatgcgt attcatataa
      781 tatattttat ttttgattag attatttcta catcgcctac aggtatggaa acacaaatct
      841 gtatggtttc gggtgtggct tctgaaaatt tttgggttaa tgtattgtag actatgcctt
      901 tgttttttgt acactggcat tccttgcata agcccattct gcatccgtgt ttcggatttt
      961 caccatgtgc ttccagttgc tccagtatgg ttttacttcc ggctgtatat atttctttgt
     1021 ttttatatat tattttggat ttatttttat tgaacttaaa atgaacagga tacaaaacag
     1081 gcttgaaata ttctgaaaag atgttgtctg catctacgct gttatgcttt aatagcattg
     1141 ttgtgtcact tatcatcgta tttggaccac aaataaaaac ttgtctgaag taataatcag
     1201 gacaatattt ttctaactgc tgtttggaaa gtctgccttc agtagttgat gacatataat
     1261 ggacggtgat ttgtttgtta agcgtcaatt tatccaactt atttctgaaa atatgctgtt
     1321 tgtcttttgc ataataaagc aacactgcat tcgtgttttc atttgcacat ttttgcagca
     1381 tagcataaaa tggagtaata ccacttcctc cggcaatcat caaaacgggt ctgtttttat
     1441 cgtgtaaaat aaattcgcct ttggcatcac ttatttttat tatactgcca acttccagat
     1501 tttcaaacag ccagggtgtt accttgcctt gatcctgttt ttgtatggta agtgttatcg
     1561 ttccggaaga tttatattca tcttccgatg aagaaatgct gaatatacgg gtataattta
     1621 cggcattgat ttttagctgt gtttcaacat actgtccggc tataaagcca ttccattttc
     1681 tgtttggttt caatttgatg gtaagcatat tttcgttttc cttatttaca gaaacaactg
     1741 ttgctttagt tgaaacaagt gatttggatg gaaaatattt ttcgaccaaa ggctcaaaat
     1801 aatacgatgc tttagtatta aacagcatgt tgtttaactg tgtgcgaatt tcattgaaaa
     1861 tgggagctgc catgttttac tttttattgt aaagtaaaat taatgccaat cagaaaccga
     1921 tagattaact ttgggtaagg aagtctgttt aacattttta taaaacagca ggctattttg
     1981 acatcattct gctttttaat cgactgaaag attcaggtgt aataccgata taagatgcca
     2041 gttgttgctg tggcacacgg ttgattactt tgtcatgttt ttctaaaaaa tataaatatc
     2101 gctgctcagg attgagggtg ataaattctt ctacccgatc aatcaattcg agtattaaac
     2161 tttccaaaac aatcatgatg cttttaccca cttctgcatc attttttgcc aattctttta
     2221 atacagaata atcaatcact gctatgatag aatcttctaa agcttgatag gatatggtag
     2281 atggcttatt ttgtaagata cttctgtgag aggcaaaaac agtaaactct tctctgaacc
     2341 agaaggtgtt ttctatatca ttgtacttat aataagcacg gaagagaccg tcgataacaa
     2401 acacaatttt accataaaat tgcccggttt taataatctc tgatcttttg gagatttgct
     2461 gaaattcaaa atgaggaatc aattccatga ttttagcata acttaaatcc gggaagcttt
     2521 tttttattcc atcgctgtaa attctaaatt ggtcgttggc aaaattatcc atatcgcaat
     2581 ttatgaagtt ctaatataca aaattacttg taaatgcata atcaagctta attgataaat
     2641 atgacatcaa gtcaatgcaa atgacatcac acatttacta agcctgatta tagcttgata
     2701 atctgctcaa caaataatgg caattactta tgccggtgta tcctgtttag gttgttttct
     2761 tgaaaggtaa aagatgaaaa atcctgcaat gataaatgga atactcagta attgaccatg
     2821 cgtaaatgtg aagctgccga aatgaaacgg attgcgatcg tattttacac tttctataaa
     2881 gaatcttcca ataaacatga tgaacagaaa aacagacatt aatgtacctt cacgaggttt
     2941 atagtttgct cttttataca taatcaacaa cacaatgaag ataaaatcat aaaggaaagc
     3001 ctcaaataat tgtgcaggat agcgaggtgt aggaatgccg ttgtcttccg tgcattgcat
     3061 gaaaataacg ccccagttgg gattattcgt gggtatcccc acaatttcgt gattgaagaa
     3121 gttgccatat ctgacaaaag atgcaccaag tgctgttgga atagataccc tgtcgataat
     3181 ccaaagatac gattgatccg gatgtctgcg tttatataga tataaggcaa tcggaatagc
     3241 tatggcagca ccgtggctgg ctaatccgcc tttccacacc tgaagaattt caagcagatg
     3301 gtctttgtaa taatcccaat cgtaaaaaaa acaatgtcct aagcgtgcac caatcaatac
     3361 cgcaaaaaac atgtacatca gcaattcctc agctccttca ggatttttat tttctttttt
     3421 aaattcccat tgcaatatat aatagcctaa taaaaatgcc aatccatata ataaactata
     3481 ccaacgaatg ccataagaat ctgtaatttg tattattaca gggttggcat tccaatgtat
     3541 aacaaacgaa agaaaattaa tcatactcta ttttgaattg caaaaataag gattttaata
     3601 gtttaacttg ttaaaatgtc taaatgatgc tactatttta taacattcat tttacaagct
     3661 tccgattata tttgtacata tcagaatgat tgaccttgaa aaatcaacca atttcatttc
     3721 atgtgaacat tgccgatgca gacgatttgg tctatgtgca agagttatgt aatctatatg
     3781 aagaatctgc aaaaaaacgc ggaacaggga tagccaaacg cgatccgaaa taccttgaat
     3841 taaaaattct ggaatcaaaa gcaattattg ctaagacaaa caaaggagag cttgccggat
     3901 tttgttatat cgaaacatgg gaaggcaaaa actacgtggc taattcaggc ttgattgtta
     3961 aagaaaattt cagacaccac ggacttgcca aacgtatcaa gaaatttgtt tttgaataca
     4021 cgcaaaaaaa atatccgaat gcaaagatat ttggtatcac aaccagtctg gctgtaatga
     4081 agctcaattc tgatttaggc tatcgtccgg ttactttttc cgagctaaca caggatgatg
     4141 ctttttggaa tggttgtcaa agctgcataa attttgatat tttaagtcgt acacatcgaa
     4201 aaaattgttt gtgtaccggt atgctttttg acccgaaaga agataaaaag aaagtgtttg
     4261 ccaaggcgca gaaagtagaa agaaaaattc cggcaaaact caaaacaccc aaagcgcaac
     4321 gcattaaagc cgtaaaaaaa tgagctatct tcaattaact gattagataa tgccatgatt
     4381 taattaccag ccacgtagtg gcaacacctt atccgtattt aataaattta atttatcctt
     4441 aatgcctgtt caacttaatc cgagagcaaa tctataagcc tttacgtatt tttgtttaaa
     4501 gctgaatatc cgttcagctt attatttaat atattctatt tatgaagatt ttaaaatttg
     4561 gtggcacatc cgtaggctca gttcaaacta ttcgtatgat tattgacatc atccggaaag
     4621 aaaaagaaaa ccatgaggat ctggttttgg tttgttctgc atttagtaaa gtaaccgacc
     4681 agctgattaa tgcgggaaaa ttagccgaga aaaaagacaa cactacctat ctcgaaatat
     4741 ttgaaagttt taaagaaaga catcgccttg cgcttgaaga actagtgagt aataatgaga
     4801 taaaatcggt gatgctggaa gaatttttcg acagtatgga aaatgtaaaa gacattctaa
     4861 aaggcataca ccttttagga gaactatcag aaaaaacatt agcccagttg gtttcttacg
     4921 gcgaacgttt ttcttgcttt atcgttgccg gagcattgcg taatgcagga ttggaaggaa
     4981 tttatgtaaa tgccaccaag ctggtacgca ccgattcaaa ctatctttct gcaaaagtaa
     5041 attttgacaa aaccaataaa aatatactcg acttttttga agcaatacat ggcactgcag
     5101 ttataaccgg atttataggt gctaatgaac atggtacggt tactacgctt ggcagaggag
     5161 gttcagatta taccgctgct atctttggag cggcactaaa ggcagatgtt attgaaatct
     5221 ggacagatgt agatggtgta cttacagcgg atcctcgtcg ggtagatcag gcatttactg
     5281 tgcccatgtt atcctataag gaagctatgg agttatcgca ctttggtgct aaagtaattt
     5341 atccgccaac catacaacct gcttatatca agaatattcc gattattata aaaaacacgt
     5401 ttaacccaga acatcccggc acgtttattt caaaagaatc cggtaaatca aataacgcca
     5461 tcaaaggcat ttcttctatt tctgatattg cagtcttgcg catggagggt accggtatgg
     5521 tcggtgtggt aggtattgca tcgcgcttgt tcagtgcatt gtcaagagca caaatcaata
     5581 tcatttttct tacacaaggc tcgtcagaac attctatttg tttcggtatt ttgcccaacg
     5641 aaatcaagaa agcacataaa gcggttgaag aagaattccg ttatgaaata caaagcaaac
     5701 atatagctcc actggttgtc gaaatcaaca attctattat agccgtaatc ggcgaaaata
     5761 tgtcgaacgt gccgggtgtt tctgctaaga tgtttaaagc attaggaaat aacggtatta
     5821 atgtaaaagc catagcacaa ggttcttctg agctaaatat tacagcagtc attgataaac
     5881 acaatgaagc aaaagctttg aatgcattgc acgaaatctt tttctcaacc gacaaacatt
     5941 ccatcaacgt atttttggta ggaccaacag gcttaatcgg caaaacgctg ctaaaccaaa
     6001 tcagaaaaca atttgatttt ctaaaaaatg aaaaaaatat tctcatcaat gttaccggaa
     6061 ttatcaattc taaaaaaatg ttgattgata gtaatggtat agatttgtct gattgggaaa
     6121 atgaattaaa tgaaaatgga gatgaatcaa atttggcgca ttttactgaa aaattaatcg
     6181 atctcaattt tgccaattcc gtattgattg attgctcagc aagtaagcaa gttgtggagt
     6241 attatgaaaa attgttgaag aaaagtataa atatcgttac accaaataaa atagccaata
     6301 ccttaagtca ggaaaaatat aaccgactcc gtgagctggc taaacgagcc aacgtacatt
     6361 ttatgtatga aaccaatgta ggagcggggc ttcctgttat tggggtgttg gcatcattga
     6421 tgaattcggg tgatgaaatt ttaaaaatag aagcagtgct ttcgggtact ttgtctttta
     6481 tttttaatac ctacaaagac aatttaaaat tcagtgatgt ggtacttact gcaaaagaaa
     6541 aaggctttac tgagcccgac cctcgcgatg atttatccgg tttggatgtt gcccggaaag
     6601 cacttatttt atcaagagac agtggcttgc aaatggaact ggatgatatt aaagtgaaaa
     6661 atttagtacc tgaaaatttg cgcaacgtaa gtgtggctga attcatggat cgcctacctg
     6721 aattgaatga cgaatatgaa aagttgaaaa aatatgcaga agccaatcaa gctgcattgc
     6781 gctacatggc agtgattgaa aatggtaaag tagaaattgc attgaaagaa gtagatatca
     6841 accatccgtt ttataatctg agtggtagcg ataacatgat tgtatttact accgagcgat
     6901 ataaaaacaa tcctttggtt attaaagggc ctggtgccgg tgctgaagta acagctgccg
     6961 gtgtctttgc agaagtgatt accataggaa actacctggc taattagcgt tgaatgtaga
     7021 attttaaatt tttaattatt ataatattat ctaaaacaag ttgaatatag tattatttag
     7081 tgtcatgtaa ttaagcttgt catgccgaaa cgcataggaa agcatgggca tcagcgaagg
     7141 atgattattt ttgcatgatt ttttgtttct tttagctgga aaaaaagaaa tgcaaataaa
     7201 attatatttt tgtaacagtt agcttagtgc agaagcgtat ttatctcaaa taatttaatc
     7261 atattgaata taatgacagc taaatctccc aaacgaattc gtgtatttgc ccccgccaca
     7321 gtagccaatg ttgcttgcgg ttttgatgta ttgggctttg ccatagacaa acccggcgat
     7381 gaattgatta tggaaattac tccggaaaag ggagtgcaaa tagtggacat agaaggcgat
     7441 gatggcgtat tgccgcgtga tgcaaaaaag aattcagcaa cagtggctat tcaggattat
     7501 ctggatttta tagatgccga cttcggttgc aaaatctggc tgaaaaaaat gatgccaagt
     7561 ggaagcggtt taggaagtag tgccgccagt gctgtagccg gagtttttgc cattaatatg
     7621 ttgtgtgatg agcagctgac taaggaggaa atgttgccct tcttactcca tgcagaaaaa
     7681 gcagcgtgtg atgcagccat tgcagataat gtagcagcca gtttgttcgg tggttttatt
     7741 ttggttcgca gttatgaacc tttggatatt ttgcaaatac ccgttcccga agatttatgg
     7801 tgcgcagtca ttaatccgca tgtaattgtt ttgacaaaag aagcccggga aattttgcct
     7861 aaagaaattc ctttagcgca ttctttacga caaagtgcga atgttggagg tttgatggtt
     7921 ggtttactac ggggagatta tgatttaatc ggacgaagtt tgattgatta tatcgcagag
     7981 ccatatcgca gcaagctcat tcccggtttt tatgatatga aaaatgctgc cattgaagcc
     8041 ggggcattag gttccagtat cagtgggagt ggacctagtg tatttgcatt atgtcatggc
     8101 aaagaagcag cacaaaaagt gggtgatgcc atgaaaaatg taatggataa actcaatatt
     8161 ggagcagatg tttatgtgag caaagtgaat aaccatggac caaaagtatt accactgagt
     8221 aatgattaat gcttaatgac gaatgatgaa ttttaaacta tgaattatga ccaattaccc
     8281 atggctaatc actaatgacc aatcactaat aaccaatcac taaccatcaa tgcttggact
     8341 aaaattagcg actgaccctc gatgggtgaa tttggcagag aaatccatcg aagaaatcct
     8401 gacagatcat gcttattgtg agcaaaaagc cacctcggct tgtatttcgt taattcagct
     8461 ttttccagat aaaacgtata tggtagataa gctagtgcct gtagtgcagg aagagtggat
     8521 gcattttaag ttggtattag atgaattgaa aaaacgcaat ttaaaactag gacaacaacg
     8581 caaagatgaa tatgtaaatg cattgttaaa attcatcacc aattcaagag aaaaaatatt
     8641 gctggacaga ttactgatga gtggcttgat agaagccagg agctgcgagc gctttcgcct
     8701 gttatcctta gagatttcgg atgatagttt aaagcagttt tatcataatt taatgcgtgc
     8761 cgaagcatta cattaccgca tgtttttgga catcgcagaa caatattacc caaaagataa
     8821 agtaaggaaa cgctgggaag aatggctcag ctttgaaacc aaagtgctga aagcaattga
     8881 actccgtggc gaccggatgc attaactaag gtatattact gtctgtttaa tataatcaag
     8941 aataacctga gttcagataa gcaattcaca gcctttcaat ttctattaaa tgtcgtaaaa
     9001 aatgacactc attaatcgaa acagctaccc aactttcagg taattaactt ttttgcttag
     9061 ttataattac gctccagcac tgacttatgt accaatgctg ccaaaattcc gcctagtatt
     9121 ggtgccaccc aaaataacca aacttgagat agtgcattgc cacctgcaaa taatgccggt
     9181 cctaagctac gtgcaggatt tacagaaact ccggttactt ttatgcctac aatatgaatt
     9241 aatactaaac ataagccaat ggctactccg gcaaaacctc cgtttatatt tttagtactt
     9301 gtagctccaa agatggtaag taggaaaatg aatgtaaata ctaattcagc aataaatgct
     9361 gcagtgctgt tatattggcc taaataacct tcaccccatc catttgcccc taaagcccat
     9421 tctttcattt catatcctcc tattgcgttg ctgacaataa gataaaggat gcccgtacca
     9481 agtattgcac caatacattg agaaatgaca taacctatag tgtcagtaac acttaatttt
     9541 ccggcaacca gcatagaaat agaaattgca ggattaatat gacatcctga aacatgacca
     9601 atggcgtatg ccatagccac cactgataaa ccaaaagcaa atgctatacc taataaaccg
     9661 acaccggttg tgccgtctgc tccagcaata actgcacttc cacagcccat aagaaccaat
     9721 accatggtgc caagcatttc ggcgaggtat ttagaatacg atttgatttc catatgctat
     9781 atttttacct aaatgtatta atattaattt aactttgcaa aataaaaaaa gacagaatcc
     9841 tgagattctg cctgataatc aaatatttaa atttttattg aactgtatta ccttgatggc
     9901 taatggatta ttttcacttt ttaatacaag caaaatttat ccacatatac caatttacag
     9961 cgataatgtg cttaaaacat cattcatggc acgaacagca tcggcgcttt tgttaaatgc
    10021 agcttgttct tcttcagtca atttgtaatc aataatttct tcccagccgt ttttaccgat
    10081 ttttaccgga acgcctaaac agatatcttt ttgaccatat tcaccatcta aataaacaca
    10141 tgatgtaatc acacgttttt cgtctcttaa aatagcctca acaacactca tgccggctgc
    10201 ccctggagca taccaagcag aagtacctaa taatttagtc aaagtagcac cgcctaccat
    10261 agtatcagca gccactttat ccaaagtttc tttatctaat aattgtgtta ccggaacgct
    10321 gtttaaattg gctaaacgtg tcaacggaat cattgtcgta tcaccatgtc ctcctataac
    10381 aactgcattt aaatcattta acgaacagtt tagtgcttgt gataaatagt atttaaaacg
    10441 agagctgtct aatgcacctc ccatacctat tactctgttt tttggtaagc cggtagcttt
    10501 cagtgccaga taggtcatgg tgtccatagg attggaaata atcacgaaaa tagtattagg
    10561 agaatatttc aagatgtttt cacaaactcc ttttacaatt ccggcattag tgccaattaa
    10621 ttcctctctt gtcattcctg gtttacgggg aatgcctgat gtaataacca ctacatcgct
    10681 gtttgctgtt ttggaataat cattggtaga gcctactact ttggtgtcaa aacccaaaat
    10741 ggcagaagtc tgcatcatgt ccatggcttt tccttccgct aagccttctt taatatcgag
    10801 taatactaac tcactgcaga gttctttgcg agcaatattg tcggcaacgg ttgcaccaac
    10861 tgcacctgcg cctactacgg taactttcat atcagataaa atttagtgtt aaaaaatcgg
    10921 cgcaatttaa gaaaagtgta cggcaaaaaa tcgaaaaaca taagatgttt atgttatttt
    10981 tcagcttaaa aattaaagag ataaaagtat taaaccacat gattgtttac ctttgctgtt
    11041 atgacagact ttagtatcat ccagaaatat aatgtgccga tccctcgata cacaagctat
    11101 cctactgttc catactggca ggacaacaag tcatcaaatg aagaatggaa agcctgtatg
    11161 ctcaaagcga taaataatgg cagaactaaa aacggtatca gtatctatat tcatcttccg
    11221 ttttgtgaac aactttgtac ctattgtggt tgtaataaac gaatcactaa aaatcatagt
    11281 gtagagggag tttatcttga ggcagtattg aaagaatggg aaatgtataa agctgtttta
    11341 ggtactgact ttactataca agaaatccat ttaggcgggg gcactcctac atttttcagc
    11401 ccggagaatt taaaattgct gatacaaaat attacaaaag atatccgaat tgctccagat
    11461 catgaattca gttttgaagg acatccgaat aatacgactt atgcgcactt gaaaacttta
    11521 tttgatgtag gttttaacag agttagtttt ggtgtgcagg atttaaacga aaaagtacag
    11581 gtagccatta atcgggtaca accctttgaa aagacgaaac aagtaacaga ttgggcaaga
    11641 gaaatcggat atacgtctgt taattttgat ttgatttatg gcttgccatt tcagacctta
    11701 gacagtatta aggacaccat ttcaagagtg attggtctaa agcctgaccg attagcattt
    11761 tacagttatg cgcatgtgcc ttggacaaaa aaatcacaac gtgcttatga tgaaaatgac
    11821 ttgccaaagg gaaaagataa atttgcactt tatacagcag gcagagagtt gtttttagct
    11881 gcaggatatg aagatgtagg catggatcat tttgcattaa aagatgacgg aatgttacaa
    11941 tgcaaacggg agggtaaact gcatcgcaat tttatgggtt acacgaccaa caatacggag
    12001 atattgattg gtttgggtgt atctgccatc agtgatgttt attatggtta tcgtcagaat
    12061 tataaaactg ttgaagaata ttatgaggcg gtaaacaaag aggagtt
//
LOCUS       DAOQII010000049        11994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2660_length_11994_cov_5.400787, whole genome shotgun sequence.
ACCESSION   DAOQII010000049 DAOQII010000000
VERSION     DAOQII010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_11994_cov_5.400787"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLF48_03765"
     CDS             <1..627
                     /locus_tag="PLF48_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48474.1"
                     /translation="DNIVVEEKGIYSIEKFLVARRLMYWQVYLHKTSLVADLMLKNAF
                     LRAKELFQDGHKISNMPSHLFALLEENYKDENELLSLFTQVDDADAICSLKSFMLQDD
                     EILKRLSSGLINRDLFKIEFLPENIADEKYNQISPQFNEDERSYFLLNGEVMNNAYNP
                     KKENIFILFKDGSIRDIAEIAEQMNIRSLSNEVVKYYLAYPENNLVLN"
     gene            640..1107
                     /locus_tag="PLF48_03770"
     CDS             640..1107
                     /locus_tag="PLF48_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF48475.1"
                     /translation="MLQPNVQALLAIYKTEIQNLIQLIEPLSADELSKIRDNETLDED
                     CKSIKTIVEHLIFSGYCYTNYYQVNVGDVLPRPEKQNYGDSSANLANELEVMYQHCEA
                     FFIEHPDISITELDNANKIKTRWGQVYDIDQLFEHSIVHILRHHRQISNFLKQ"
     gene            1182..2165
                     /gene="lpxD"
                     /locus_tag="PLF48_03775"
     CDS             1182..2165
                     /gene="lpxD"
                     /locus_tag="PLF48_03775"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNF48476.1"
                     /translation="MFTLSEIATILHGSVVGDEQHFIKRPAKIEDAQADEITFLANEK
                     YIPYLKTTQAGCVVISTNLYDSPYIFEGWKSDKNFILVEDAYVAFTQLLHLAEQNTKH
                     EVHSANYKIGKDTVIYEHVFIGNNVQIGDNCIIYPNVSIYKDCVIGNNCIIHSGAVIG
                     SDGFGFVQQQNGEYHKIPQLGNVIIEDNCEIGANTCIDRATMGSTIIRRGVKLDNLIQ
                     VAHNVEIGEHTVIASQTGISGSTKIGHHNRIGGQVGFVGHISIAPYTQINAKSAVAGN
                     ITKENQALSGIPVMDIKLHFKAIAAYKQLPELLQRVRNLEAEISEIKKKDS"
     gene            complement(2257..3921)
                     /locus_tag="PLF48_03780"
     CDS             complement(2257..3921)
                     /locus_tag="PLF48_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF48477.1"
                     /translation="MKYVIIGGVAGGASAAARLRRLDESAEIILFEKGAYISYANCGL
                     PYYIGDVIKERNKLFVQTAASFKQRFNIDTRVLSEVIAIHKEEKKIEIKNLTSGEIYT
                     ESYDKLVLSPGADPVRPPLPGIQEEGIFTLRNVSDTDKIKAYVQQFPKGKAVVIGAGF
                     IGLEMAENLHHLGMQVSIIEMINQVMAPADFTMASIVQQHIRSKGVNLRLNTAVAGFE
                     RRDGKLNVLLKDGNSIATDVVILSIGVRQDTKLAAEARLQIGQARGIVVNEYLQTSDD
                     DIYAVGDAIEFKHPISGNMLTTYLAGPANKQGRICADNIVMGNVEKYKGSIGTAIVKV
                     FDLTVAVSGMAAKHLKAANIDHIVSTTHSGSHAGYYPDAEQMSIQLAFSPGEGKVLGA
                     QIVGKTGVDKRIDILSSVIQRGSDIHELTEFEHAYAPPYSSAKDPVNMAGFVAENILD
                     GKHEIIYWDKIQDLSENDLLIDVRSAEEYKRGSIPNAINISVDDLRTKLNELPKSKNI
                     YVFCQIGLRGYLAQRILLQHGFAHVYNLSGGYKLWENAYKETMLVG"
     gene            complement(3918..4268)
                     /locus_tag="PLF48_03785"
     CDS             complement(3918..4268)
                     /locus_tag="PLF48_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF48478.1"
                     /translation="MRKKTTDCPFTFTLSILEGKWKLPIINILLQNGTMRFKELERGI
                     DGITARMLTKELKELEEKKIVKRKAYATVPPTVEYTLTECGMTLKQIIQHMHKWGEVY
                     NKMNSKQKNQIKKV"
     gene            4413..5291
                     /locus_tag="PLF48_03790"
     CDS             4413..5291
                     /locus_tag="PLF48_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNF48479.1"
                     /translation="MENKMRTIEKILTPPAPHMVGNGFRVHNFFPHGDITQKRISPFL
                     MLDYNSKVEFPPSEIPRGVGVHPHKGFETVTIAYQGRVEHHDSGGNSGVITERGVQWM
                     TAGSGVLHKEYHEKEFSKKGGLFQMVQLWVNLPKISKLTPPKYQDIPEEKMSKVQINA
                     HSHADVIAGELNGVKGSATTFSPVNLFNLYLSKDTVYRFAIPKEHNTALLVVEGSINM
                     QDAENIPTDNFILMRNDGTEIELKATEDAIVLVMSGEPINEPIASYGPFVMNTTAELY
                     EAIDEMNAGKFGTLED"
     gene            complement(5339..6982)
                     /locus_tag="PLF48_03795"
     CDS             complement(5339..6982)
                     /locus_tag="PLF48_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNF48480.1"
                     /translation="MYKHFIFILIIFIFNILGNAKNPSNTQRPKLVVGIVVDQMRWDY
                     LYRYKDRYTKDGFLRLINSGFNCEQTFIPYTPTVTAAGHASIYTGSVPAINGITGNNF
                     YEYNTKQSLYCTQDDSVLTVGANNNNGKMSPKNMLTTTIGDELQLAQNFKGKVIGISI
                     KDRGAILPAGHSATAAYWYDNKTGNFISSTYYMQALPDWVNAFNSLKKADDYYNKGWT
                     TLFPANTYTNSDTDMNSYESAPFGSEQVGLPYSFTSILEKKNYGALRVTPYGNSITFD
                     FARETIIKEKLGKSNITDMLAVSFSSTDYIGHTFGPNSVEMEDTYLRFDKELSDFISF
                     LDQQIGKNNYTLFLTADHGAAHIPAYLKTKKIPAGGLALSKVEKQMNEHLRTKFAVDS
                     LVLAIENYQVFFDKEKLKQHAITLNDVMQDAVDFLIQYDEIDRVLDYTEIAEETLPDV
                     MKSMFTNGYYPNRCGQLLLVLKPSFIDDEYGSKGTTHGLWNPYDAHIPLLFYGKNIQK
                     GKSTEKVFMTDIAPTICTLLHIQMPNGCIGNVIEGVEKK"
     gene            complement(7156..7842)
                     /locus_tag="PLF48_03800"
     CDS             complement(7156..7842)
                     /locus_tag="PLF48_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48481.1"
                     /translation="MNYQVIIDREKLKHFIEWLPDLERGEAYYCSLLARSKYCKDNAH
                     ISSDKQQLKRFTSTKEFLFEKIKQLECELGCYYQKHFPLPQEALAIYINPNPRSYEKA
                     AKEGLKKLADLVTKPYSDYNPHQEIMSEIQKAYSRKVFYDIDFDGVELEMVRTKLNGI
                     INADCLHFLQTRGGFHLLIELAKIDEQYQKTWHQSIAKMEGVDVKGDNLIPIAGCYQG
                     GFVPSFYETK"
     gene            complement(7920..9290)
                     /locus_tag="PLF48_03805"
     CDS             complement(7920..9290)
                     /locus_tag="PLF48_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48482.1"
                     /translation="MKPLLIVINILLVLHTRLLNAQDTVQTIVLNNTLSDDNHIVVHK
                     NRTRSFMNGNKHYPKLDTVRNKYIRWIDKHNREFPFAIPLLAYNKYDGMQIGAGFVNL
                     KQPVKHIDFTASLFYGIDSKKVSGTADINYYFRPKKGVVTEIRPGVNFKSFSYENFRK
                     PLKYYAISPEINLTFKYRSEKYENLEYQLLLKNYVILNKALNYYMDGSTLKYKDTLTH
                     FYANSLTFQFKRTDENFPFECSLNLEQTSDFVKTSFTANAFIRYQLKKMNTGVHIRFF
                     AGGFLWRSNDFKFRLYPEVGFALSGKTGKDDYLYEDFYIGRNEQEKFASRQISESDGY
                     FKVMTPLNAIPLGETVNGMFAVNIKLDFPIQYVPLKLFFDMGYSVDRKLNPDNFLPVK
                     GFQYDAGFMFSFLDEAFEVYFPVLMSKDFKTYYKSNAPKFKQRISFNLDINKLQLHKK
                     IRDLRL"
     gene            9344..10756
                     /locus_tag="PLF48_03810"
     CDS             9344..10756
                     /locus_tag="PLF48_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF48483.1"
                     /translation="MSDFEFENEDFFNIDELVQQYEDAQRKNKSRFFDQEDFESIIEY
                     YQFSGRYDEAMQVSDESLKQHASSAILWIKRAQLYFELKNIEMALIALDTAGNYDPNE
                     IEIDLLKAEIYTFQSKYQEAVDILESLLDKAERDEKIDVYLQLCDVYEDWEKYPQVYD
                     YLLLCLALDDQNSEALNRLNYVVEITNQFEESIHLHQKIIDQHPYNYLAWYNLACAYR
                     GTHQIEKAIEAYEYVIAINDKVDYAYTEIAEIYYNTKEYKKALDILKDLCELMEPDDE
                     VFFMQGKCFEAMGDMKMARYNFRKCVHENPANSQAYFRIGETYKQEGQWQQAYKAFQK
                     AADLEKEQYEFCLAMAEAAMEINEIEVAVEACETAIDIFINRHEAYFILASIMALNDD
                     TETALEIIDKGIGISKSKLELRYAQAAIYVMENKLKHAEMLLTELLTEDEFEVYNCMF
                     DFCPALEENVWLNNLIERFY"
     gene            complement(10999..11169)
                     /locus_tag="PLF48_03815"
     CDS             complement(10999..11169)
                     /locus_tag="PLF48_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48484.1"
                     /translation="MGFIKEPEGVDFVIKSRTLTKAEKVALSKFIAESKAKDKKKTAT
                     KKAVSKTNKAVV"
     gene            complement(11205..11702)
                     /locus_tag="PLF48_03820"
     CDS             complement(11205..11702)
                     /locus_tag="PLF48_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48485.1"
                     /translation="MAKSKKYHLDFEIDKLTNSIENTFTGDSFQTEIIRLSRSDLKLI
                     TKEKGWLFDWKYEHHQNDRDVYKLTIQNNPTIIQGLISFTVNSDHVYMHLLESAPFNL
                     GKNKIYNGVPGNLVAYACKVSFQHGNDGFVSFTSKTQLIHHYETTLGAYHFGGQLMVI
                     EKKAA"
     gene            complement(11738..>11994)
                     /locus_tag="PLF48_03825"
     CDS             complement(11738..>11994)
                     /locus_tag="PLF48_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNF48486.1"
                     /translation="RLGFSDEEAQKQFGFLMNAFEYGAPPHGGLAFGFDRLCAVMNGV
                     DSIRDYIAFPKNNSGRDMMLDSPSTIDEKQLKELCIKTTV"
BASE COUNT         3842 a         2138 c         2124 g         3890 t
ORIGIN      
        1 gataatatcg tggtggaaga aaaagggatt tattctatcg aaaagttctt agtggcaaga
       61 cgactgatgt actggcaggt atatttacat aaaacgagtt tggtggctga tttgatgttg
      121 aaaaatgcgt ttttgagagc gaaagagtta tttcaagatg ggcataaaat atcaaatatg
      181 ccatcacatt tatttgcatt gcttgaagaa aattacaaag acgaaaacga actcttatcc
      241 ttgtttaccc aagtggatga tgccgatgct atatgcagtt taaaaagttt tatgttgcaa
      301 gatgatgaaa ttctaaagcg cttatcctcc ggactaatca accgggattt atttaaaatt
      361 gaatttctgc ctgaaaatat agccgatgaa aaatacaatc agataagtcc tcaatttaat
      421 gaagatgagc gcagctattt tttactcaat ggcgaagtaa tgaataacgc ttataatcct
      481 aaaaaggaaa atatttttat tctgtttaaa gatggcagta ttcgggacat tgcagaaatt
      541 gcagaacaga tgaatattcg ttccttgtcg aacgaagtag taaaatatta tttggcctat
      601 ccggaaaata atttagtttt gaattgaaaa ataaatgcaa tgcttcaacc taatgtacaa
      661 gcacttttag cgatttataa aactgaaatt caaaatctga ttcaattaat agaaccgctt
      721 tctgcagatg aattaagtaa aataagagac aatgaaacac tggatgaaga ttgtaaatcg
      781 ataaaaacga tagtagagca cttgatattt tccggttatt gttatacaaa ctattatcag
      841 gttaatgtag gtgatgtatt gccacgtccg gaaaaacaaa attatggaga ttcatccgct
      901 aatttggcaa atgagctgga agtgatgtat caacattgtg aggctttctt tattgagcat
      961 ccggatatat ccattacaga acttgataat gcaaataaaa taaaaacacg ttggggacaa
     1021 gtatatgata tcgaccaact ttttgaacac agcatagttc atattctcag acaccacaga
     1081 caaatttcaa attttttgaa gcaataaata atcgtggttt taaaaagtga ttgattgtta
     1141 cactaaagaa ttttccgctg tatattcttt atctttagtc catgtttact ctttcagaaa
     1201 tagctactat actccatgga agtgtagtcg gcgacgaaca acattttata aaacgtccgg
     1261 caaaaataga agatgcacaa gcggatgaaa ttactttttt agccaatgaa aaatatatac
     1321 cgtatttaaa aactactcaa gcaggctgtg tcgtcatttc aacaaatctg tatgacagtc
     1381 cctatatttt tgaaggctgg aaatcggata aaaatttcat tttggtggaa gatgcttatg
     1441 tggcctttac acaactattg catttagccg aacaaaatac caaacacgaa gtgcacagtg
     1501 ccaattataa aattggaaaa gacacagtca tttacgagca tgtgtttatt ggcaataatg
     1561 tgcagatagg cgataattgt atcatctatc cgaatgtgag tatctataaa gattgtgtaa
     1621 tcggcaataa ctgtattatc cattcgggtg cagtaatcgg cagtgatggc tttggatttg
     1681 tacagcaaca aaatggggaa taccataaga taccacaact tggtaatgta attattgaag
     1741 acaactgtga aattggtgcc aacacctgta tagaccgcgc cacgatggga tctacaatta
     1801 tccggcgtgg agtgaagctc gataatttaa tccaggtggc acataatgta gagattggag
     1861 aacatactgt catagcatcg caaaccggca tatccggttc tacaaaaatt gggcatcata
     1921 atcgcattgg tgggcaagtg ggttttgttg gtcatatcag tatagctcct tatacgcaga
     1981 ttaatgccaa gagtgccgtg gcaggaaata ttaccaaaga aaaccaggca ttatccggta
     2041 tcccggtcat ggatatcaaa ctgcatttca aagctattgc tgcatacaaa caacttccgg
     2101 aactcttgca acgagtaaga aatttagagg cagaaatttc agaaatcaaa aaaaaggaca
     2161 gctaatcagc atgaataata ctaattagct gtaataactt caagtatttt tttagtctaa
     2221 tgatttctct ctatatgtaa acaagcataa cgggttctac cccaccagca tcgtctcttt
     2281 ataagcattt tcccataatt tatatccacc tgaaagatta taaacatgtg caaatccatg
     2341 ttgtaataaa atgcgttgag ctaaataacc gcgcaagcct atctggcaaa acacataaat
     2401 atttttgctt ttaggcagct catttaattt cgttcttaag tcatctactg aaatatttat
     2461 cgcgtttggt attgagcctc gcttatattc ttctgcagat cgcacatcta tcaacaaatc
     2521 attttcactc agatcttgta ttttatccca ataaataatt tcgtgttttc cgtccaaaat
     2581 attttcagct acaaatcccg ccatattgac cgggtctttt gccgatgaat aaggtggggc
     2641 ataagcatgt tcaaattcag ttagttcatg tatgtcactt cctctttgaa taactgacga
     2701 taaaatatct atacgcttat ctacaccggt tttacctact atctgcgcac ctaatacttt
     2761 cccttctccg ggagaaaagg caagctgaat gctcatctgt tcagcatccg gataatagcc
     2821 cgcatgagag ccactgtgtg tagtagatac aatatgatct atattggcag cttttaaatg
     2881 cttggcagcc attcctgaaa ctgcaactgt caagtcaaat acttttacga ttgctgttcc
     2941 gattgagcct ttatattttt ctacatttcc cattacgata ttatctgcac aaattctgcc
     3001 ctgtttgttg gcaggccccg ccaaatatgt ggtcagcata ttaccgctta taggatgttt
     3061 gaactcaatg gcatcgccta ctgcataaat atcatcatca gatgtttgca gatattcatt
     3121 gacaacaatt cctcttgcct gtccaatttg caatctggct tctgcagcaa gtttagtatc
     3181 ctggcgaacg ccaattgaca gtataactac atcggttgca atggaattgc catcctttaa
     3241 caagacattt aattttccat ccctgcgctc aaatcctgca actgcagtat tcaaacgcaa
     3301 atttactccc ttggaacgaa tatgctgctg tactatacta gccatcgtaa aatctgcagg
     3361 agccatcact tgattaatca tttctataat gctgacttgc atacctaaat gatgcagatt
     3421 ctcagccatt tccaaaccga tgaatccggc accgattacg actgccttgc ctttaggaaa
     3481 ttgctgtaca tacgctttta ttttatccgt atcacttaca ttacgcaagg tgaaaattcc
     3541 ttcttcctgt atgcccggta agggcggacg aacggggtcg gctcccggcg ataatactaa
     3601 cttatcataa ctttcagtat agatttcacc agatgttaga tttttgattt caatcttctt
     3661 ttcctcttta tgtatggcta ttacttcaga aagtacacgt gtgtcaatat taaaacgctg
     3721 cttgaaggat gcagcagttt gaacaaataa tttgtttcgt tctttaatta catcgccaat
     3781 gtaataaggt aagccgcaat tggcataaga gatgtaagcg cctttctcaa aaagaatgat
     3841 ttccgctgat tcatctaaac gtctaaggcg cgcggcagca ctcgcgccac ctgctacacc
     3901 gccaataatt acatacttca tactttcttt atttgatttt tttgttttga attcatttta
     3961 ttataaactt ctccccattt gtgcatgtgt tgtatgattt gttttaaggt cataccgcat
     4021 tctgttagcg tgtactctac agttggagga acggttgcat aagctttgcg ctttacaatc
     4081 tttttttctt ctagttcttt caattctttg gtgagcatac gtgcagtaat tccatcaata
     4141 cctcgctcca gttctttaaa acgcatggtg ccattttgca gcagtatatt gataatgggc
     4201 agtttccatt ttccctctaa tatggatagt gtgaaggtaa atgggcagtc agttgttttt
     4261 tttctcattt ttaacaattt ttaaatatca tttagtaact tgattatcag cattacttac
     4321 accgagtata ttgatataca ttcagtatac tacttgtaaa agtatagtaa ctatactaaa
     4381 tttgtcacaa atataaacat aaaggaataa tcatggaaaa caaaatgaga acaattgaga
     4441 aaattttaac accaccagct ccccacatgg ttgggaatgg tttccgcgta cataattttt
     4501 tcccgcacgg agacatcact caaaagagaa taagcccatt tttaatgctg gattataatt
     4561 caaaagtaga gtttccacca agtgaaattc ctcgcggagt aggcgtgcat ccgcataaag
     4621 gatttgaaac cgtaacgatt gcttatcaag gccgggtaga acatcatgac agcggtggca
     4681 acagcggtgt aataacagaa agaggtgtgc aatggatgac ggcaggaagt ggtgtattgc
     4741 acaaagaata tcacgaaaaa gaattttcta aaaagggagg tctttttcaa atggtgcaat
     4801 tatgggtgaa tttaccaaaa ataagtaaac tgactccgcc taaatatcag gatattccgg
     4861 aagaaaaaat gagtaaggtg caaataaatg cacatagtca cgcagatgta attgcaggag
     4921 aactgaatgg agtaaaaggc tctgccacta ccttttctcc cgtgaacttg tttaacctct
     4981 acttgtcaaa agataccgtg tatcgctttg ccatacctaa agaacataat actgctttat
     5041 tagttgtaga agggagtatc aatatgcaag atgcagaaaa tataccaact gataatttta
     5101 ttctgatgcg gaatgacgga acagaaattg agctaaaagc aacggaagat gctatcgttt
     5161 tggtaatgag cggtgagcca attaatgagc ccatcgcatc atacggacca tttgttatga
     5221 ataccacagc cgaactttat gaggctattg atgaaatgaa tgccggaaaa tttggcacac
     5281 tggaggatta atgtctaaaa aaatatatta taaaattaaa atagtcagaa atacaaaatt
     5341 attttttttc tacgccttcg atcacatttc caatacagcc attgggcatt tgtatgtgca
     5401 acagggtgca aatggtaggc gcgatatccg tcataaatac tttttcggtt gattttcctt
     5461 tttgtatatt tttgccgtaa aacaataatg gaatatgtgc atcatacgga ttccataaac
     5521 catgtgttgt gcctttgctg ccatactcat catcaataaa tgaaggcttt aataccaata
     5581 ataattgacc gcaacgattt gggtaatatc cgtttgtaaa cattgatttc ataacatctg
     5641 gcaatgtttc ttctgcaatt tctgtgtagt ctaatacccg atcaatctca tcatattgta
     5701 ttaaaaaatc tacagcatcc tgcattacat catttaaagt tatcgcatgt tgcttcaatt
     5761 tctctttatc aaaaaatacc tgataatttt caattgccaa tactaaggag tctacagcaa
     5821 atttagttct cagatgttca ttcatttgct tctcaacttt tgataaagct aagccgcctg
     5881 cgggtatttt tttcgttttc agataagcag gtatatgtgc tgcgccatgg tcagctgtta
     5941 aaaatagcgt gtaattattc ttaccgattt gctggtctaa aaatgaaata aaatcgctca
     6001 attctttgtc gaaacgcaaa taggtgtctt ccatttcaac agaattaggt ccaaatgtat
     6061 gaccgatata atctgtggaa gaaaatgaaa cagccagcat atcggtaata ttgcttttgc
     6121 caagtttttc cttaattatg gtctctctgg caaaatcaaa tgtgatggaa ttgccataag
     6181 gcgtaacacg cagtgcacca taatttttct tttcaagaat tgaagtgaat gagtaaggca
     6241 agcctacttg ttcactcccg aatggggcac tttcatagct attcatatca gtatcactat
     6301 tcgtgtatgt attagcagga aataaagtag tccacccctt attgtaataa tcgtcagctt
     6361 tcttcagtga gttgaatgca tttacccaat ccggtaatgc ctgcatatag taagtagatg
     6421 aaataaaatt acctgttttg ttatcatacc aataggcggc tgttgcacta tgccctgccg
     6481 gcaaaattgc tcctctgtct tttatagaaa taccgattac tttgcccttg aaattttgtg
     6541 ccagctgcag ttcatcaccg atagtggtag taagcatatt tttcgggctc atcttgccat
     6601 tattattatt tgcacccacc gtcagtacgc tgtcgtcttg tgtacaataa agcgactgct
     6661 ttgtattata ttcataaaaa ttatttccgg taataccgtt gatggctggt acagaaccgg
     6721 tataaataga tgcatgtccg gcagcagtta cggtgggtgt gtagggtata aaagtttgtt
     6781 cgcagttgaa gcctgaattt atcaacctga ggaaaccatc tttagtatag cggtctttat
     6841 aacggtacaa ataatcccaa cgcatctggt caactacgat gcctacaacc agtttgggtc
     6901 gttgtgtgtt tgacggattt ttagcatttc ctaaaatgtt aaaaatgaaa ataattaaaa
     6961 taaatataaa atgcttgtac atatttgtta atcaaaaagt gaaaaatgat cttcgtgtaa
     7021 gatataaaat caggaagtta agttatgcaa gaagaggcaa attagaaaat attcttaaat
     7081 aaaaatggta gcatccaatc atcttgaatg aaaacaagtt aaatattatt tatattctat
     7141 tatatagtat tgattttatt ttgtttcata aaatgaaggc acaaaaccgc cttgataaca
     7201 accggctatc ggtatcaaat tgtcaccttt tacatcaaca ccttccatct ttgcaattga
     7261 ctgatgccag gttttctggt attgttcgtc aatttttgcc agctctataa gtaaatgaaa
     7321 tccgccacga gtttgtagaa aatgcaaaca atctgcattg atgatgccat taagttttgt
     7381 acgaaccatt tctaattcta cgccatcaaa gtcgatgtcg taaaatactt tcctactgta
     7441 agctttttgt atttcagaca ttatttcctg gtgcggatta taatctgaat atggtttggt
     7501 tactaaatct gctaattttt taagtccttc tttagctgct ttttcgtagc ttctggggtt
     7561 gggattgata tagattgcaa gcgcctcttg cggcaagggg aaatgttttt gataataaca
     7621 acctaattca cattctaatt gctttatttt ttcaaataaa aattctttcg ttgaagtaaa
     7681 gcgtttcagc tgttgtttat cagaacttat atgtgcattg tctttgcaat attttgagcg
     7741 agccaataaa ctacaataat atgcctctcc tctttccagg tcaggaagcc attctataaa
     7801 atgcttaagc ttttccctgt cgataattac ttgataattc attctggtat aaatataatc
     7861 tttttgaaaa aaatagaagc ttaagtgaac gaacatctct catttcttac atcttgattc
     7921 tacaagcgca aatctcttat ctttttatgc agttgtaact tgtttatatc cagattaaat
     7981 gaaatacgct gtttgaattt cggtgcattg gatttgtagt atgttttaaa atctttactc
     8041 atcaatacag gaaaatatac ttcaaaggct tcatctaaga aactgaacat aaatcctgca
     8101 tcatattgaa aaccttttac aggcagaaag ttatccggat ttaatttgcg gtcaacagaa
     8161 taacccatat caaaaaataa tttcaacggt acatattgta tcgggaaatc caatttgata
     8221 ttcaccgcaa acatgccatt gactgtttct ccaagcggaa tggcatttaa tggagtcatc
     8281 actttaaagt atccgtcact ctcagaaatc tgccgtgatg caaacttttc ctgctcattt
     8341 cttccaatat aaaaatcttc atataagtaa tcatcctttc ctgtttttcc gcttaaggca
     8401 aatccgactt caggataaag tctaaatttg aaatcattgc ttctccataa aaatcctccg
     8461 gcaaaaaacc ggatatgcac gccggtattc atcttcttta actgataacg aataaaagca
     8521 tttgcggtga aacttgtttt tacaaaatct gaagtttgct ccagattcaa gctgcattca
     8581 aaaggaaaat tttcatcagt tcttttaaat tgaaatgtca gactattggc ataaaaatgg
     8641 gtaagtgtat ccttatattt cagcgttgaa ccatccatgt aataattcaa tgctttattt
     8701 aaaatgacat agtttttcaa aagcaactgg tattctaagt tttcatattt ttcacttctg
     8761 tatttgaagg ttaaattgat ttcgggagaa attgcataat atttcaatgg cttcctgaaa
     8821 ttctcatagc taaaagattt aaaattaacg ccgggtctta tttcagtaac aactcctttt
     8881 ttcggacgga aataataatt tatatcagct gttccactca ctttttttga atcaatacca
     8941 taaaaaagag atgccgtaaa atcaatatgt tttaccggtt gttttaggtt gacaaatccg
     9001 gctccaattt gcatcccgtc gtatttatta tatgcaagga gcggtattgc aaatggaaat
     9061 tcacggttat gtttgtctat ccagcgaata tacttattgc gcaccgtatc taatttcgga
     9121 tagtgcttgt ttccattcat aaaagaacga gtcctgtttt tatgaacaac gatatgatta
     9181 tcatcactta atgtattatt gagtactata gtttgaacag tatcttgcgc gttgagcaaa
     9241 cgagtatgca atacaagtaa aatgttaata actattaaca gtggtttcat gagaatagat
     9301 tattttaaat ctattacaaa ataagcatta actttgaaat agtatgagcg attttgaatt
     9361 tgaaaatgaa gatttcttca atattgatga attagtgcaa caatatgaag atgcgcaacg
     9421 aaaaaacaaa agccgatttt tcgatcagga agattttgaa tctattatag aatattatca
     9481 gttttcgggc agatatgatg aagccatgca ggtatcggac gaaagtttaa aacaacatgc
     9541 gtcttctgct attctctgga taaaaagagc gcagctttat ttcgaattaa aaaatatcga
     9601 gatggcactt atagcactgg atactgcagg aaattatgat ccaaatgaga ttgaaattga
     9661 tttgctgaaa gctgaaatat atacattcca gtcaaaatac caggaggcgg ttgatattct
     9721 ggaaagtttg cttgataaag cagagcgcga tgaaaaaatt gatgtctatc tgcagctctg
     9781 cgatgtttac gaagactggg aaaaatatcc tcaagtatat gattatttgt tactttgctt
     9841 agcacttgat gatcaaaatt cggaagcttt aaaccgcttg aattacgtag tagaaattac
     9901 caaccaattt gaagaaagta ttcatcttca tcaaaaaatt atagaccagc atccctataa
     9961 ttatctggcc tggtataatc tggcttgtgc atacagaggc acacatcaga ttgaaaaagc
    10021 aatagaggct tatgaatatg tgatcgccat taatgacaaa gtagattatg cttatacaga
    10081 aattgcagag atttactata acacaaaaga gtataaaaaa gcactcgaca ttctaaaaga
    10141 tttgtgtgaa ctgatggaac cggatgatga agtttttttt atgcagggaa agtgttttga
    10201 ggctatgggg gatatgaaaa tggcgcgtta caatttcagg aaatgcgtgc acgaaaatcc
    10261 ggcaaactcg caagcttact tccgcatcgg cgaaacctat aagcaagagg gacaatggca
    10321 acaagcttat aaagcctttc agaaggctgc cgatttggaa aaagaacaat atgagttttg
    10381 tctggcgatg gctgaagctg ctatggaaat caatgaaata gaagttgccg tagaagcttg
    10441 cgaaacggcc atcgacatat ttattaaccg ccacgaagcc tattttattt tggcgagtat
    10501 catggcactc aatgatgaca cggaaacagc actcgaaatt attgacaagg gcattggaat
    10561 cagcaaatca aaattagagc tgaggtatgc gcaagcggca atttatgtaa tggaaaacaa
    10621 gctgaaacat gcagaaatgc ttttaacaga attactgaca gaagatgagt ttgaggttta
    10681 caattgtatg tttgattttt gtcctgcact ggaagaaaac gtgtggctga ataatttgat
    10741 cgagcggttt tattaactga tttacgttta gtttcatatc agtgtcttaa atagtctatt
    10801 gctttttgat taggtttata atttcaacca tcggcaacag tgtttcttaa tggtattgtc
    10861 aacttatgga tttcaaaatc tgaaagtagt attattgaca ctaatgcttt ttcccagatt
    10921 aagcaaataa ataaaactta cacagtcgtc ttgatacaca actctttcag ttgtttttca
    10981 tctatggtgc ttggtaaact aaacaacagc tttattggtt tttgaaacag ctttttttgt
    11041 agctgtcttt tttttgtctt ttgctttgct ttcagcaatg aatttactta aagctacttt
    11101 ttctgctttt gttaaggtgc gactttttat tacaaaatct acgccttctg gttctttaat
    11161 gaatcccatt ttatttaatt tttaaaatat ttatcaacta aaatttatgc tgctttcttc
    11221 tctattacca ttaattgacc accaaaatga tacgcaccta gtgttgtttc ataatgatgt
    11281 attaattgag tttttgaagt aaaagaaaca aaaccatcat tgccatgctg aaaagaaact
    11341 ttgcaagcat aagctactaa attacctggt actccattat aaattttatt ttttcctaaa
    11401 ttaaaaggag cactttctaa caaatgcata taaacatggt cgctatttac agtaaaactt
    11461 attaatcctt gaataattgt cggattattt tgaatggtta atttataaac atctctgtca
    11521 ttttgatggt gttcatattt ccaatcaaat aaccatcctt tttctttggt aattaatttt
    11581 aaatcagatc ttgacaagcg aattatttct gtctgaaagc tatcgcctgt aaaggtgttt
    11641 tcaatagaat ttgtcaactt gtctatttca aaatctaaat gatatttttt gcttttagcc
    11701 acatataaat ttacggaaaa aatatcaaaa agacagctta caccgtcgtc ttgatacaca
    11761 gctctttcaa ctgcttttca tcaatagtac taggcgagtc gagcatcatg tcgcggccgc
    11821 tgttgttttt aggaaaggca atgtaatctc taattgagtc aacaccattc ataacagcac
    11881 acaatctatc gaaaccaaat gctaagccac catgcggagg tgcaccgtat tcaaacgcat
    11941 tcatcaaaaa tccgaattgc ttttgcgctt cttcatcaga aaatcctaaa cgct
//
LOCUS       DAOQII010000050        11963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2672_length_11963_cov_5.082214, whole genome shotgun sequence.
ACCESSION   DAOQII010000050 DAOQII010000000
VERSION     DAOQII010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_11963_cov_5.082214"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PLF48_03830"
     CDS             complement(<1..1203)
                     /locus_tag="PLF48_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF48487.1"
                     /translation="MRILLPFLFLISFFSTFGNTRYYIQFKDKNSNADLKKEFSAKAL
                     EKKARLNIAFDKRDIPVNEQYLLALRTDGIVVESVSKWLNTAIIVVPDGKDLLKYKFI
                     TSIYAIRDVKTKIHKKGISSLEECLEEAVYDDSVYINSYPQFHIMDGEYLHSLGFNGE
                     NMTIAVCDAGFQNANTMGAFAQLYSQGRIKGTYDYVHNDSSVADPSDHNHGTMCLSTI
                     AGWIADKYKGGAINADFYLFQTENASTERIIEEYNLARALERCDSLGVDVVSISLGYT
                     TFDVSSEDHDSLMMMQNSTPAAKAVNMAVSKGMVVCVAAGNEGNGSWHYISTPSDADS
                     AIAVAAVDMGGNPASFSGWGLAQDSRVKPNVACVGAGTAVISPSNTVVYGSGTSFACP
                     SMAGFVTCL"
     gene            complement(1217..1999)
                     /locus_tag="PLF48_03835"
     CDS             complement(1217..1999)
                     /locus_tag="PLF48_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48488.1"
                     /translation="MKKSLILLFGLVLALVSCKNETENYNASVDDNYFPDDSGAWVEY
                     RVDSVVYNDFYYAFPPSSPQPQYVHTSTHYVKQVVGEKFTDNMGRQATKIYRYTKDSL
                     SQPYDDVPRVWYFVKTKTNVEVVEENLRYIKLVFPANVDLSWKGNKYVTDTFPFVPLK
                     GFFQINYNGWDYAVSEKDKIYNNGFHTFDSVLTVVQYDDSTNVYKVSSVEKYARNTGM
                     VYREFWRLDAQQDTDDNTPLIPFMQSKLNGFIVKQYAINYGH"
     gene            2107..2592
                     /locus_tag="PLF48_03840"
     CDS             2107..2592
                     /locus_tag="PLF48_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48489.1"
                     /translation="MNNTVGAYFKQLTILFLAICTGCCFFMGMFYFLVYQSAKSTPNT
                     GNAIFEIAGLVIGFGLAIAARFLFFNTTKKALSLSSLKEKLSYFRTAVLLQLAMLEGG
                     VLLNTIFYYLNKNSFNLAVAGGLLFLMIMRRPTRPIAAMVLFNPQENNRVVYDDNQLI
                     D"
     gene            complement(2582..3487)
                     /locus_tag="PLF48_03845"
     CDS             complement(2582..3487)
                     /locus_tag="PLF48_03845"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNF48490.1"
                     /translation="MNFFKSKALKQIESLHPVKDAWKIVQLTLFYEFPWDYNRALELA
                     LYKTFAVPSISKILMQSGQFTNFAQKRYDDTDLLLSEIIENGMSPKGLEAIAHMNWIH
                     SNYKISNEDYLYVLSTFIFDSARWINTYGYRQLTKNEEIAGFYVWLEIGEQMQIKHIP
                     PSIQAFKKYHDEYECDHFLFDEKNKKLAITTENLMLSWYLPKSMYDFFRPVLHAVMEP
                     HLLKSFDFKEPHHIMQYAITQVLKTRSSIASLFPRNEPLLRTKEPHRYYKNGYQLYDI
                     GPDKLKKTCPYHTVKEQVSKLAVNL"
     gene            complement(3539..4168)
                     /locus_tag="PLF48_03850"
     CDS             complement(3539..4168)
                     /locus_tag="PLF48_03850"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF48491.1"
                     /translation="MLKYIFPIGTLILMWLIYLTQLISGVDFGFLGIHPRDMQALLGI
                     LTAPLVHGNWEHLLSNTLPLLILSMILFLSYQRIALFVWCGIYFFTGLLVWLFARDSF
                     HIGASGIIYGLASFLFFNGFFRMDIKAIAIASGVAIFYGGMVWGVLPIYPGVSWESHL
                     FGGITGAVMAYIFRNYNKDTTLQQMEDEHQAYIDQRKTFDSFLDDLNKR"
     gene            4245..4832
                     /locus_tag="PLF48_03855"
     CDS             4245..4832
                     /locus_tag="PLF48_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48492.1"
                     /translation="MQKTTFLFPETMYHIFNRGTNKQSIFLDENDYAKFFFLWEKYAE
                     PIMDIYAYCLLPNHFHFLVKIKSEKELRRLKEFKDIDNERITYRVSNTISNLFNAYSR
                     YFNLKYDRSGKVFTERFKRKPIVTDAYFSKLIEYIHKNPEKHELVEDFRDYAYSSYWL
                     HLIDQPTRLKVNEVLNWFGGRDEYVNFHNNTYEVV"
     gene            4896..5999
                     /locus_tag="PLF48_03860"
     CDS             4896..5999
                     /locus_tag="PLF48_03860"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNF48493.1"
                     /translation="MLIGIPKETKFKENRVAITPLIAKDLLAKGFQVSVETEAGLNSF
                     FSDEDYKNFGATIVPDKKDIYANADIVLKVNAPLPDEIALMKKGAVLISFMWAKTNTE
                     MVDACAAAGISAFSMDAVPRISRAQKMDALSSQANLAGYKAVLLCANEIGKILPMMTT
                     AAGTIKPSKVVIFGAGVAGLQAIATAKRLGAIVEVSDIRPETKEQVESLGGRFIEVKG
                     DDSIKMEGGYVKGVSEEFLKKQQELVAKHVADADIVITTALIPGKKAPVLVTEDMIKS
                     MKFGSVILDMAVEQGGNVVGSKLNETIQLDGVTIIGESNIPSLLPMNASDLYAKNIQT
                     LLYHLADKDGFKWEMEEEITKGSLIVYNGNKLI"
     gene            6049..6351
                     /locus_tag="PLF48_03865"
     CDS             6049..6351
                     /locus_tag="PLF48_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNF48494.1"
                     /translation="MEFLDFFLANLDMVYFIILAIFVGVEVIGGVPTVLHTPLMSGAN
                     AIHGVVIVGAIIVMLDMDPNNVFGLALGFLAVLLGTLNVVGGFVVTDRMLEMFKKK"
     gene            6436..7830
                     /locus_tag="PLF48_03870"
     CDS             6436..7830
                     /locus_tag="PLF48_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNF48495.1"
                     /translation="MKGILEIAYLIASVTFILGLKMMGNAKTARRGNLIGAFGMTVAI
                     LATIFLYRTDSTTTNTIKFVLIFGAIAIGTAIGWLTAKRVEMTKMPELVSMFNGMGGA
                     CAALIGLSEYQHNVNHTGKLAFIIAGLVIGTISFSGSIIAYLKLAGKMNKPIRFPQYN
                     ILNNLVLLGVLVFGVFITLDGAALPAWIVWVLFAVAAVYGILFTIPIGGADMPVVISL
                     LNSFTGLAAAFGGFLYDNKPMLTGGILVGSAGTLLTLAMCKAMNRPLSNVIFGAFGGG
                     NSAAAGGTDAQGSIKDMSVSDLAVLLNYSSKVVIVPGYGLAVAQAQHVIHELEKLLEE
                     RGVEVKYAIHPVAGRMPGHMNVLLAESNVEYDKLVEMEDINPELDKTDVVLVVGANDV
                     VNPAAKTDPSSPIYGMPILEVDKAKNIIVNKRSMNAGYAGIDNLLFYNPKTSMFFGDA
                     KKALTELVAEIKQL"
     gene            8205..8636
                     /locus_tag="PLF48_03875"
     CDS             8205..8636
                     /locus_tag="PLF48_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48496.1"
                     /translation="MYYVAKRQITIFGKEEFAWYYPKDPDAHWWFGWTPEGYEDPKEA
                     FKYLSKPLGQFADVLEKSFLVTFGTLSGYEAGVLATSIWGASYEFIKQYGLQGGDMKE
                     IDWANVAIEGAPLPKGLGKIGGEIVRDLFEAGVDYKNEKKL"
     gene            8890..9249
                     /locus_tag="PLF48_03880"
     CDS             8890..9249
                     /locus_tag="PLF48_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48497.1"
                     /translation="MSKQNWINTLLFILIGFIFLYFLGVVKRNDINKNAAYSCGKIYD
                     YYEDTKGHSNILFKYYVEKKEYTYMYVGGNFKDCEKTGWCIGKCFVVQYSSKNPENST
                     MHFDKPCNCDSLNVDIP"
     gene            9340..9552
                     /locus_tag="PLF48_03885"
     CDS             9340..9552
                     /locus_tag="PLF48_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48498.1"
                     /translation="MDYKNGNFYIAGTEEKTITEASIEFFSSAISSLVIGKYEKQLKS
                     AFKDTDPKYTKEIIEQFVKFGNEYIK"
     gene            9638..9997
                     /locus_tag="PLF48_03890"
     CDS             9638..9997
                     /locus_tag="PLF48_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48499.1"
                     /translation="MNKNDWIAIVASFIFLSIFIIFYKNRKDKDIKENHSISCGTISN
                     YYFTTKGITTINYFYFVNGKQVTGGAGNAYFKDCEETGWCIGKCFVVEYSSKNPENSR
                     MNFDKPCNCDSLNVDIP"
     gene            10088..10300
                     /locus_tag="PLF48_03895"
     CDS             10088..10300
                     /locus_tag="PLF48_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48500.1"
                     /translation="MDYKNGNFYIAGTEEKTITEASIEFFSSAISSLVIGKYEKQLKS
                     AFKDTDPKYTKEIIEQFVKFGNEYIK"
     gene            10386..10745
                     /locus_tag="PLF48_03900"
     CDS             10386..10745
                     /locus_tag="PLF48_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48501.1"
                     /translation="MNKNDWIAIVASFVFLSIFIIFYKNKKDKDIIENYSITCGTISD
                     YFYTTKGITKIEYFYYIKGKKVKHIAGNAYFKDCEKTGWCIGKCYIVEYSSKNPENSR
                     MNFDKPCNCDSLNVDIH"
     gene            11146..11574
                     /locus_tag="PLF48_03905"
     CDS             11146..11574
                     /locus_tag="PLF48_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF48502.1"
                     /translation="MNKVSSILSRKKVAFIGLQESATVLDALKLMLEKNIGSVIVMSG
                     DTYLGIVTERDYSRKVVLAGKHSDQTTVAEIMSEFPKISPDDTIERCMELMSDKNIRY
                     LPVFENEKLVGLISVSDVVAETIRQQKTTISHLEDYIKGS"
     gene            complement(11586..>11963)
                     /gene="msrB"
                     /locus_tag="PLF48_03910"
     CDS             complement(11586..>11963)
                     /gene="msrB"
                     /locus_tag="PLF48_03910"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNF48503.1"
                     /translation="KTDEQWKQVLSTEEYRVLREAGTEYPYTGKYTDFDEQGNYYCKA
                     CGNLLFSSDTKYHSGCGWPAFYDIATGDAVITRQDTSHGMNRIEVLCKKCHSHLGHVF
                     PDGPLDKTGLRYCINSVSIDFKK"
BASE COUNT         3962 a         2047 c         2280 g         3674 t
ORIGIN      
        1 caaacaggtt acaaaacctg ccatagacgg acatgcaaat gatgtaccac ttccatatac
       61 cactgtattt gacggactga ttactgcagt gccggcaccc acacaagcca cattaggttt
      121 tactcttgaa tcttgtgcca agccccatcc gctgaaagat gcaggattgc cacccatatc
      181 tacagcagct actgcaatag cactatccgc atcggaaggc gtggaaatat aatgccagct
      241 gccattgcct tcattgccgg cggctacaca aactaccata cctttggaaa cagccatatt
      301 aacggcttta gcagcaggag ttgaattttg catcatcatt aaactgtcat gatcttctga
      361 agaaacatca aacgtagtat atcctaagga aatagaaaca acatctacgc ccaatgagtc
      421 gcatctttcc agtgctcttg ccaaattata ttcttctatg atgcgctcgg ttgaagcatt
      481 ttccgtttga aataaataga agtctgcatt aatcgcacca cctttatact tatcagctat
      541 ccagccggct atggtagata aacacattgt accgtggtta tgatcagatg gatcagccac
      601 ggatgaatca ttatgtacat aatcgtaagt acctttaatt ctaccctggc tgtacaattg
      661 agcaaatgca cccatagtat ttgcattctg aaatccggca tcacaaacag ctatggtcat
      721 gttttcgcca ttaaaaccca aactatgcaa atattcgcca tccataatgt gaaactgcgg
      781 gtaagagttg atataaacac tgtcgtcata aaccgcttct tccagacact cttctaaact
      841 gctgattcct tttttatgta ttttggtttt tacatcccga atggcatata tcgaggttat
      901 gaatttatac ttcaataaat ctttaccatc cggcacaaca atgatagcag tgttcaacca
      961 tttggaaact gactctacga caataccatc tgttctcagt gctagtaagt actgttcatt
     1021 tacaggaata tcgcgtttat caaatgcaat atttaatcgt gctttttttt caagtgcttt
     1081 tgcagaaaat tcttttttca aatccgcatt ggaattttta tccttaaact gtatgtaata
     1141 tctggtattg ccgaatgtgg aaaaaaacga aatcagaaat aaaaaaggta aaagaattct
     1201 catgagatgg tattgcttaa tgtccgtaat ttattgcata ttgctttacg ataaatccat
     1261 ttaatttact ttgcataaag gggattaaag gtgtattgtc gtctgtatct tgttgtgcat
     1321 ctaacctcca aaattctcta taaaccatgc ctgtgtttct tgcatatttc tcaacagagc
     1381 ttactttata tacattagta gaatcgtcat attgcacaac tgtcaatacg gaatcgaaag
     1441 tatgaaaacc attattgtat atcttatctt tctctgaaac tgcataatcc cagccattgt
     1501 aattgatttg aaaaaagcct tttaaaggaa caaatggaaa agtgtcggtt acatacttat
     1561 tgcctttcca gcttaaatct acatttgccg gaaatacgag ttttatataa cgcaggtttt
     1621 cttcaacaac ttccacattt gtttttgttt tcacaaaata ccatacacgc ggtacatcat
     1681 catagggctg tgacaaactg tctttggtat aacgatatat tttagtggct tgtctgccca
     1741 tattatccgt aaacttttca cccactactt gctttacata atgtgtcgat gtatgtacat
     1801 attgtggttg cggagaagaa ggaggaaagg cataatagaa atcgttatag acaacagaat
     1861 caacccgata ctctacccat gcaccggaat catccggaaa ataattatca tcaaccgaag
     1921 cgttataatt ttcagtttca tttttgcagg aaaccaaagc aagtacaagt ccgaaaagca
     1981 aaatcagcga tttcttcata ctataaaaat atactataaa gttggaaaat taagtcattt
     2041 agttgttttt acataaaaaa attgcgcact ttttgataat acgttatttt taatcaaaaa
     2101 tttgacatga acaatactgt tggtgcatat ttcaaacaac tgactatcct gtttttagct
     2161 atttgtacag gttgttgttt ttttatggga atgttttatt ttctagttta ccaatctgct
     2221 aaaagtacac caaacaccgg aaatgcaatt tttgaaattg ccggtttggt gattggattt
     2281 ggcttggcta tagctgcccg ttttttattt tttaatacta caaaaaaagc attgtcatta
     2341 tcttccctta aggaaaaatt gagttatttc cgcactgctg ttttgttgca attagccatg
     2401 cttgaaggcg gcgtgttatt gaataccatt ttttattatc taaataaaaa ctcttttaat
     2461 ttagctgttg ccggaggttt actttttctg atgataatgc gtcgacctac tcgtcctatt
     2521 gcagcaatgg tattatttaa tccccaggaa aacaatcggg tggtgtatga tgacaatcag
     2581 cttatagatt aactgccaat ttacttactt gttctttcac ggtatgatac ggacacgttt
     2641 ttttcaattt atccggaccg atatcataca actgataacc atttttataa taacgatgtg
     2701 gctctttagt acgcaacaat ggctcatttc tcggaaatag tgatgcgata ctgcttcttg
     2761 tttttaatac ctgtgttatg gcatattgca ttatatgatg tggctccttg aaatcaaaag
     2821 attttagcag atgaggttcc atcacggcat gtaataccgg tctgaaaaag tcatacatag
     2881 atttgggtaa ataccagctt aacatcaggt tttcggtagt gatggctaat tttttatttt
     2941 tttcgtcaaa caaaaaatgg tcgcattcat actcgtcatg atatttttta aaagcttgga
     3001 tgctaggcgg aatatgctta atctgcattt gttctcctat ttccagccat acgtagaagc
     3061 cggcaatttc ttcatttttt gtaagttgac gatatccgta agtatttatc caacgtgccg
     3121 aatcaaaaat aaacgtactc aatacatata aataatcttc gtttgaaatt ttgtaattgg
     3181 aatgaatcca gttcatgtgt gcaatggctt ccagaccctt tggcgacatg ccgttttcaa
     3241 taatttcact caacaataaa tcggtgtcat cgtatcgttt ttgtgcaaag ttggtaaact
     3301 gcccggactg catcagaatt ttagaaatag aaggaacggc aaatgtttta tacaaagcca
     3361 attctagagc acggttataa tcccagggga attcataaaa caaagtcagt tgcacaattt
     3421 tccatgcatc ttttaccgga tgcaaagatt cgatttgttt cagtgcttta gacttgaaga
     3481 aattcattat ctccaagtta atacagaaaa cgaatactta caagcgaata acgattactt
     3541 atcttttgtt taagtcatct aaaaaggaat caaatgtttt tcgttggtct atataagcct
     3601 gatgctcatc ttccatttgt tgtagcgttg tatccttatt gtaatttctg aaaatgtaag
     3661 ccataacagc tccggtaatt cctccaaata aatgcgactc ccacgataca cccggataaa
     3721 tgggcaatac cccccacacc attcctccgt aaaagatggc cacaccactg gctatggcta
     3781 tggcttttat atccattctg aaaaatccgt tgaaaaacaa aaacgaagcc aatccatata
     3841 taataccact ggcaccgata tgaaatgaat ctctagcgaa gagccaaacc agtaatccgg
     3901 taaaaaaata gataccgcac cagacaaaga gagctattcg ctgataagaa agaaacaaaa
     3961 tcatacttag aatcaacaag ggcaacgtat tggaaagcag atgttcccag ttgccatgta
     4021 ccaaaggggc tgtcagaatt cccaataaag cttgcatatc tctcgggtgt atgcccaaga
     4081 acccgaaatc aactccggag attagctgag tcaggtaaat cagccacatt agtatgagcg
     4141 tgccgatagg aaatatatac ttcaacataa tttatgacct cgtaggttaa atttatggaa
     4201 tttcgttcat tttcgactct aaatataaac aaacctcgta ggtcatgcaa aaaacaacat
     4261 ttttatttcc ggaaacgatg taccatattt tcaacagggg tactaacaaa cagtctatat
     4321 ttttagacga aaacgattat gcaaaattct tttttctatg ggaaaaatat gccgaaccga
     4381 taatggatat ttatgcttat tgtctgcttc ccaatcattt tcatttttta gttaaaatca
     4441 aaagcgaaaa agaattgagg cgattgaaag aatttaaaga tattgataat gaaagaatta
     4501 catatcgtgt atcaaatacc atttcaaact tatttaatgc atattctcgg tatttcaatt
     4561 taaaatatga tcggtcaggc aaggtattta cagagcggtt taaacggaaa cctatcgtta
     4621 cagatgccta cttttcgaag ctgattgagt atattcataa aaatccggag aaacacgaat
     4681 tggtagaaga tttcagagat tatgcatata gttcatattg gttacatctt attgatcaac
     4741 ctacgaggtt gaaagtaaat gaagtattga actggtttgg tggcagagac gaatacgtca
     4801 attttcataa taacacctac gaggtggtat aatttcacgc atttaatttt aatttatagc
     4861 agaacatccg tatttttgta aaaattcgat ttagtatgct gatcggtatt cccaaagaaa
     4921 ccaagtttaa agaaaacaga gttgctataa ccccactaat tgccaaagat ttactagcca
     4981 aaggattcca ggtttctgtt gaaacggaag ccggcttaaa ctcctttttt agtgatgaag
     5041 actataaaaa ttttggtgca acaatagttc ctgataaaaa agatatttat gcaaatgcag
     5101 atattgtatt gaaagtaaat gctcctcttc ccgatgaaat tgctttgatg aaaaaaggag
     5161 ccgttttgat ttcatttatg tgggcaaaaa ccaatactga aatggtagat gcctgcgctg
     5221 cagccggaat atctgccttc tctatggatg ctgttccccg tatttcgaga gcgcaaaaaa
     5281 tggatgcctt atcttcacaa gccaatcttg caggatataa agcggtactc ttgtgtgcta
     5341 atgaaatagg taaaatattg cccatgatga ccaccgctgc cggcaccatt aaaccttcta
     5401 aagtggttat tttcggagca ggtgttgccg gattgcaagc catagctaca gcaaaacgcc
     5461 tgggggctat cgtagaagta tcggatatac gcccggaaac gaaagaacaa gtagaatctt
     5521 tgggtggacg atttatcgaa gttaaaggtg atgattccat caaaatggaa ggcggttatg
     5581 taaaaggtgt atctgaagaa tttttgaaaa aacaacaaga attggttgcc aagcatgtgg
     5641 ccgatgcaga tatcgtgatc acgacagcat tgattccggg taaaaaagca ccggttttag
     5701 ttactgaaga catgattaaa tcgatgaaat tcggttctgt aatcttagat atggccgttg
     5761 aacaaggcgg taatgtggta ggcagcaaac tgaatgaaac gatacagctg gatggtgtta
     5821 ccatcatcgg tgaaagtaat atcccttctt tattgccgat gaatgccagt gatttatatg
     5881 ccaaaaacat ccagacttta ttgtatcact tagccgacaa agacggcttt aaatgggaaa
     5941 tggaagaaga gataacgaaa ggaagcttga ttgtttataa tgggaataaa ttgatttgaa
     6001 aatactattt cctaattact tattactaat aacttataac atatactcat ggaattctta
     6061 gacttttttt tagcaaactt ggatatggta tatttcatca ttctggcaat ttttgtcgga
     6121 gttgaagtaa ttggtggtgt gccaactgta ttacacactc ccctcatgtc gggtgcgaat
     6181 gccatacacg gcgtggtcat cgttggtgct atcattgtta tgttagatat ggacccgaat
     6241 aacgtttttg gtcttgctct gggattttta gcggtattgc tcggcacgct taatgtcgta
     6301 ggcgggttcg tagttaccga tcgcatgttg gaaatgttta agaaaaaata aataaaataa
     6361 ttaatcaatg taacaatata acaatgattt tttaattgat acattgttac attttcaaat
     6421 tggtaaatta acaaaatgaa aggaatattg gaaatagcat atttaattgc ctctgtaaca
     6481 tttattttag gtcttaaaat gatgggtaat gccaaaactg cccgtcgagg aaatttaatt
     6541 ggtgcatttg gcatgaccgt agccatattg gccactatat ttttataccg cacagattca
     6601 acgacgacaa acacaataaa attcgtttta atttttggag ccatagccat cggtactgcc
     6661 ataggctggc tgacggctaa gcgtgtagaa atgaccaaaa tgcccgaatt ggtgtctatg
     6721 tttaacggga tgggtggggc ttgcgccgct ttgataggat taagtgaata tcagcacaat
     6781 gtcaatcata cgggaaaact agctttcatc attgccggtt tagtcattgg aacaatctca
     6841 ttttcgggtt ctattatcgc ctatttgaaa ttagccggaa aaatgaataa accgatacgt
     6901 tttcctcaat acaatatctt gaataatctg gtgcttttag gagtacttgt gtttggtgtt
     6961 tttattaccc ttgatggtgc ggctttacct gcttggattg tttgggtatt atttgctgta
     7021 gctgctgttt acggcatctt atttactatt cctattggcg gagcagatat gccggttgtt
     7081 atttctttgc tgaactcttt cacaggtttg gcggcagcct ttggcggatt tttatatgac
     7141 aacaagccca tgcttaccgg cggtatatta gtaggcagtg caggtacatt actgacctta
     7201 gccatgtgca aggctatgaa ccgacctttg agcaatgtga ttttcggtgc atttggcggt
     7261 ggcaatagtg cagcggcagg aggtacagat gcacaaggta gtattaaaga tatgtcagtg
     7321 tctgatttag ccgtattgct caactattca tcaaaagtgg ttattgtacc cggatatgga
     7381 ttggcggttg cgcaggcaca acatgttatt catgaactgg aaaaattatt ggaagagcgt
     7441 ggtgtggaag taaaatatgc aattcatcca gtagccggac gtatgcctgg gcacatgaat
     7501 gtactcttgg ccgaatcaaa tgtagaatat gacaagttgg tggagatgga agatatcaat
     7561 cctgagttgg ataaaacaga tgtagtgtta gttgtaggag cgaatgacgt agtgaatcct
     7621 gcggcaaaaa ccgacccgag ttctccgata tatggcatgc caatcttaga agtagataaa
     7681 gccaaaaata ttatcgtgaa taagcgcagt atgaatgccg gatatgcggg aatagacaac
     7741 ttgctgttct acaacccaaa aacatccatg ttctttggcg atgccaaaaa agccctgaca
     7801 gagttagtag cagaaatcaa acaattatag tatattagct atacccaatc gttctttgaa
     7861 aacaaaaggc ataccgctat ggatttaacg gtaaggagaa cgataatgaa gtttctggtc
     7921 aaggaaatca atatgactac ggctttagaa tttataatcc aagattaggt aagtttttaa
     7981 gtgttgaccc tttacaaagg aaatttccat tttattcagt atatcacttt gcaggaaata
     8041 gccccatagc caatatagac cttgacggat tagaaccaca attagccata tacaaatgga
     8101 attcttctcc gggttttaat aatcacggat tatattggaa agatggtaaa tgcgcccact
     8161 gtgatgaatg ggtcttaaat acagtagtag atgctgatgg tacgttgtat tatgttgcaa
     8221 aaagacaaat tactattttt gggaaagaag aatttgcatg gtattatcct aaagatcctg
     8281 atgcacattg gtggtttggc tggacaccgg aaggatatga agaccctaag gaagctttca
     8341 aatatttatc aaaaccatta ggacagtttg cggatgtatt agaaaaatca tttcttgtta
     8401 cttttggtac attatcaggt tatgaagctg gagttttagc cacgagtatt tggggagcaa
     8461 gctatgagtt cataaaacaa tatggattac agggtggtga tatgaaggaa atagattggg
     8521 caaatgttgc aattgaaggt gcgccattgc ctaaaggttt agggaaaatt ggaggtgaga
     8581 ttgtacgtga tttattcgaa gctggtgttg attataaaaa tgaaaaaaaa ttataaatgt
     8641 attagatggt agtaaaaatg gaacagaagc tacaattgaa gcagtttcaa gtgcattatc
     8701 ttcaatagtt attggaaaat acgaagaaca attaaaagcc gcatttagag ataccgatcc
     8761 agcatatatg aaacagattg ttgagcaatt tgttaaattt ggaaatgaat atatgaaaga
     8821 gattttgagt aatggaatgg ttgataaata taaaaattct aaagaagaaa agaaagcaga
     8881 atagatatta tgagtaagca gaattggatt aatacattat tattcatatt aataggattt
     8941 atttttcttt attttttagg tgtagttaaa agaaacgata taaataaaaa tgcagcatat
     9001 tcatgcggaa aaatatatga ttactatgag gatacgaaag gtcatagcaa tatattattt
     9061 aaatattatg tagaaaagaa ggaatatacg tatatgtatg taggtggtaa ttttaaggat
     9121 tgcgaaaaaa ctggatggtg tattggaaag tgttttgttg ttcaatattc ttcaaagaat
     9181 ccagaaaaca gtacaatgca ttttgacaaa ccatgtaatt gcgatagctt aaatgtagat
     9241 attccctagc aacctctatt ctagcaacca gcaacctcta cgaactttat acaataacgt
     9301 gttcggatat ccaaataaag agccctattt gaggcaggag tggattataa aaatggaaat
     9361 ttttatattg caggtactga ggaaaagact ataacagagg caagcattga gttcttttca
     9421 agtgctatat cttcattagt tattggaaaa tacgaaaaac aattaaaatc tgcatttaag
     9481 gatactgatc ccaaatatac aaaggagatt attgaacaat ttgttaaatt tggaaatgaa
     9541 tatattaaat aaattctaag taatagattg gtagagaaat ataaagcata caaagaaaaa
     9601 aaggaagctg aacaaaaaaa taagcaggaa tagaattatg aataagaatg attggatagc
     9661 aatagttgcg tcatttatat ttttatctat ttttataata ttctacaaaa atagaaaaga
     9721 taaagatata aaagaaaacc actcaatttc ttgtggcacc ataagtaatt attattttac
     9781 aactaaaggt ataacaacga taaactattt ttattttgta aacggtaaac aagtaacagg
     9841 tggagccgga aatgcatatt ttaaagattg tgaagagaca ggatggtgca ttgggaaatg
     9901 ctttgtagta gaatattctt ccaaaaaccc ggaaaacagt agaatgaatt ttgacaaacc
     9961 atgtaattgc gatagcttaa atgtagatat tccctagcaa cctctattct agcaaccagc
    10021 aacctctacg aactttatac aataacgtgt tcggatatcc aaataaagag ccctatttga
    10081 ggcaggagtg gattataaaa atggaaattt ttatattgca ggtactgagg aaaagactat
    10141 aacagaggca agcattgagt tcttttcaag tgctatatct tcattagtta ttggaaaata
    10201 cgaaaaacaa ttaaaatctg catttaagga tactgatccc aaatatacaa aggagattat
    10261 tgaacaattt gttaaatttg gaaatgaata tattaaataa attctaagta atagattggt
    10321 agagaaatat aaagcataca aagaaaaaaa ggaagctgaa caaaaaaata agcaggaata
    10381 gaattatgaa taagaatgat tggatagcaa tagttgcgtc atttgtattt ttatcaattt
    10441 ttataatatt ttataaaaac aaaaaagata aagatataat agagaactac tcgattactt
    10501 gtggtactat aagtgattat ttttatacaa ctaaaggaat tactaaaata gaatattttt
    10561 attatattaa aggtaaaaag gtgaaacata tagctggaaa tgcatatttt aaagattgtg
    10621 aaaagacagg atggtgtatt ggaaagtgct atatagtaga gtactcttcc aaaaatccgg
    10681 aaaacagcag aatgaatttt gacaaaccat gtaattgcga tagcttaaat gtagatattc
    10741 actagcaacc tctattctag caaccagcaa cctccgagaa caataataaa taaagtgttc
    10801 gcctgactgc ttgccgaatg gcaggaaagt ccaacagttg aactatgcgg tacctgcccg
    10861 cctttcatgg cggataaacc cgcttcgcag caggtctgtc tgccgtgcaa aaggcaggcg
    10921 ggtatgttat agagaaaaat accgaagtac cccaagtata agagaagaaa aaaaacacgc
    10981 taaacgggcg tgtttttttg ttctaccatt tgcttcattt tcaccaacct acgaggtgtt
    11041 aaagccaact atccaccctt aaacccttat tttattgcaa tttaataccc cacctacgag
    11101 gtcatttcag gataatgttg tatattccat aaaaattaac caactatgaa caaggtatcc
    11161 tctattttat cccgtaaaaa agtggctttc atcggtctgc aagaaagtgc aacggtactt
    11221 gatgccctaa aactgatgct ggaaaaaaac ataggctctg ttatcgttat gagtggcgat
    11281 acttatctag gcattgtaac cgagagagat tactccagaa aagtcgtatt ggctggcaag
    11341 cattccgatc aaactactgt tgcagaaatc atgtccgagt ttccaaaaat atcccctgat
    11401 gataccattg aacgttgtat ggaactaatg agcgataaaa atattcgcta tttgcctgtt
    11461 tttgaaaatg aaaaattagt cgggttgatt tctgtcagcg atgtagttgc ggaaaccata
    11521 cgacaacaaa aaacgactat cagccacttg gaagattaca tcaaaggaag ctgataattc
    11581 gcttgtcatt ttttaaagtc aatactcacc gagttaatac agtatctcaa tccggtttta
    11641 tccagagggc catcagggaa tacatgcccc aaatgagaat ggcatttttt gcatagcact
    11701 tcaattcggt tcatcccatg tgaagtatct tgtctcgtga tgacagcatc acctgtggca
    11761 atatcataaa aagcaggcca gccgcaaccg ctgtggtatt tggtatccga actaaacaac
    11821 aagttgccgc aagctttaca ataataattt ccctgctcat caaaatcggt atattttccc
    11881 gtataggggt actccgttcc agcttcccgc agcactctgt attcctcagt ggataaaact
    11941 tgtttccact gctcatctgt ctt
//
LOCUS       DAOQII010000051        11901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2688_length_11901_cov_6.013085, whole genome shotgun sequence.
ACCESSION   DAOQII010000051 DAOQII010000000
VERSION     DAOQII010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2688_length_11901_cov_6.013085"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1363)
                     /gene="nhaD"
                     /locus_tag="PLF48_03915"
     CDS             complement(86..1363)
                     /gene="nhaD"
                     /locus_tag="PLF48_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNF48504.1"
                     /translation="MTIEAILLLIFCLGYFAIIFEHKLGFDKAGAALITGVVLWTVWI
                     LASGVDDGSHRFMHEFLPEISSILLFLLGAMTIVEIIDQHDGFELIVSRITTTDKRRL
                     LWIIAWITFFLSAILDNLTTSIVMVSLLRKKIANQEDRLFFAGIVVIAANSGGAWSPM
                     GDITTTMLWIKGQITAMSIIKEIFFASAVSLLVPLLVISFIMKGEVETPKSENKETDQ
                     PIWLRWVVFLLGLGTLISVPIFKTVTHLPPFMGMLLGLGIMWVITDLLHKEKNPEDKF
                     GYSVYNAMSKIDTPSILFFFGILTAVSALDVSGILKSLALHLDNSIGNHNIIIYTIGL
                     LSAVIDNVPLVSATQSMYTFPTDHHLWHFIAYCAGTGGSILIIGSAAGVAVMGIEKIN
                     FMWYAKRIGWLAFLGYTAGAIVSVLETHYLGNI"
     gene            complement(1415..2185)
                     /locus_tag="PLF48_03920"
     CDS             complement(1415..2185)
                     /locus_tag="PLF48_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNF48505.1"
                     /translation="MVRKSIFLMWAVLFFPLNYTKSFGDENHLVIKQEQNPAVVVAET
                     EPNTTVQDYLDCFAVVSDEAMKTALSGYAYLDEKGQIQKKDIITIVDFSKPSTTERLF
                     VINLRTKKIIAKSLCAHGRNSGEIYADKFSNWAESYQSSLGFYITGETYGGKNGFSLK
                     LDGMEPGINSNARERGVVIHGADYVSYDFINANGKLGRSQGCPALPLEKNEKIISLIK
                     GGSCLFIYHPSKNYKKSSTILKHYNEDCLADVVSGFQQ"
     gene            2458..3324
                     /locus_tag="PLF48_03925"
     CDS             2458..3324
                     /locus_tag="PLF48_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF48506.1"
                     /translation="MNTKQTRTMDEIVFENRNKAYGAYVLRREVSKNEKIGFLVTILL
                     FISALYMGKLAQGLFSNGFAMVHNEGKTVTSQLADVHTEEYVRPKPEIKSEPPKELPN
                     TSPEQNVIPVTQPQQNTQDFREMNAVEDNKVNTDNLAKQIDLVDKTVGTVTTPKDPDY
                     VAVVSTPAVVTSTPEPVKVVAERPRPVAEVMPAFPGGQAALLAYLKKEMSYYSFSDNE
                     QDGKIFIRFYIDLDGSIKEATVVKDDVGGYFGKRALSVINKMPRWSPGKQGNQAVRVF
                     YTIPITYKQLGG"
     gene            3405..4244
                     /locus_tag="PLF48_03930"
     CDS             3405..4244
                     /locus_tag="PLF48_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF48507.1"
                     /translation="MSKTILITGATAGIGKETAKQLAAQGHLIYITGRDKSKCEETLQ
                     VLKQINPTAAGGYFLSDFADLSSVRKMANEINNTLPQLDVLINNAGGVFAKRELTSEG
                     YEKTLCVNHLSPFLLTDLLLTLLQKSKDGRIVTVASDSHYRGDLDFNDLHFEKNYFIL
                     KAYERSKLCNVLFSNHLAKQLEGTNVSSNSLHPGHVVSDIGAKTSNPFFKWVWKTVNN
                     LTGIPTAEGAKTSVYLASADEVASDNGYYFDKCKPKTPSKLAQEEQLAEQLWDISKKM
                     VGI"
     gene            4313..4999
                     /locus_tag="PLF48_03935"
     CDS             4313..4999
                     /locus_tag="PLF48_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF48508.1"
                     /translation="MSLQQNIKIAVDAIVFGYTQHQLHVLLVKQKYGHLKNNWVIAGG
                     FVKDDETLTQAVKRELKEEAGITVDYMEQLYTFGDDIHRDPRFRVITVAYFALVNPTK
                     LKLVADTDAEEARWFPMNEVPKLGFDHNDILIVALKRLKDKLTYQPIGFDLLPKEFPF
                     SDLENLYCSILETEIDRRNFRKKVLSFGLVEETEKYANKKEGRPAKLYRFNRQKYQQF
                     VKEGYHFEIK"
     gene            5002..5625
                     /locus_tag="PLF48_03940"
     CDS             5002..5625
                     /locus_tag="PLF48_03940"
                     /inference="COORDINATES: protein motif:HMM:NF039424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48509.1"
                     /translation="MQTQNAFYDTIVHLRLEEEAVVFTDKISISTLEERLLTSFLQQE
                     YEKEKTNYPFEAPEFQPETALWAAKMVYFAIHLHFFRQQEVAVVKNIFIPNTKEPNAA
                     QMLSADIVLRFLLPLYSDIKQVDFQDVILQGIEDCLKAYHYSAIGSDLEIQESTLDVL
                     FQQPCFRQLYLDRVTQYKSISLAKLEKIDEGLKENFGNYSSGFWQRT"
     gene            5677..6807
                     /locus_tag="PLF48_03945"
     CDS             5677..6807
                     /locus_tag="PLF48_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF48510.1"
                     /translation="MELTDKLNAVLKEIKETFVSKDEIIDLLGISLLARENAFLFGPP
                     GTAKSAIIRNLSQHIEQGKNFEYLLTRFTEPNEIFGPFDVRKLKEGELLTNTEGMLPE
                     ASMVFLDELFNANSAILNSLLMALNERIFRRGKETRKLPALMFVGASNLLPEDEALNA
                     LLDRFLIRVRCDNVEPDKLQEVLLAGWKLEQNTTQQFTTITADEIRILQQACKQINLQ
                     PIRSAYIDLIHNLRNTGIKVSDRRAVKLQSLIAASALICKRKEAVLSDLWVLKYIWDT
                     EEQVEILEGMVNAIIEKDKSEKTHPQAFYNKSPNADEVMKEVLFLQNKWKEEGLSLEE
                     KNVVKDKLRYVQSRCDWIHDREQKEYIQKEIDKLWKQILEDV"
     gene            6780..7523
                     /locus_tag="PLF48_03950"
     CDS             6780..7523
                     /locus_tag="PLF48_03950"
                     /inference="COORDINATES: protein motif:HMM:NF039751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48511.1"
                     /translation="METNTGRRIVQYIIEIPATRKDDLGSIRHWDNIKMASDADSIWI
                     KDITPRQIDTPEIRKLPQKNIYTIEQQLLFKLNGLVPTKKLPHGLLWTPIERALPIKL
                     PDHTNNYYQPEKRKIKLKTVDKEHEPDALLIEMNELERYIKTAPAIRLNKIKWIIVEG
                     KALLIGKPLLPINGISFWFKSPFFLPCGMDFEYSILQSYFMQLLDPLNENFVIWLSNQ
                     SIIRFSKYAVKPLNIGSFRLTQAQNERIK"
     gene            7520..10051
                     /locus_tag="PLF48_03955"
     CDS             7520..10051
                     /locus_tag="PLF48_03955"
                     /inference="COORDINATES: protein motif:HMM:NF039748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HNF48512.1"
                     /translation="MTELHKYFEHSTEYIWTWEVDEISPQQNVFILAVPHSFTIIYLN
                     ELVDILEFLSPQGVPILGALLLSVLATGNNAADALSVVNLRIQKLLALYPVDTHIRND
                     VKDAFELLHKIKNLPPEYKTNDKRRLVFQVLFRDCHNKLSATRARSLIKEINRNEYRI
                     DEYAPPFEFDMAILKRDFQTLSIQNKKFGNTQNIMQAIAKMLDEEMLAEYWDEEMIDE
                     EEIQPKNFIDQLSMHPETFRIGVLIKRIWGGLRIPLHQQIPSGQPLGGISDMTNKGDF
                     DRLLLSEFANDDDVFLSRIANQEALFIQREVPPQQDKITRYLLIDITLKNWGTPKIIA
                     YALSLAIAKHPKNEITCKYFVLGNKHKEIYLEDLDSVIFSFKQLSSKLDLTDGLQSFF
                     EEEQIDDHAEIFILSSPNTIFSAANQQALQTYRAYFHYLITADANGVINLYKYGSRNK
                     RHLQTIQLPLEELWKNAPVKISRKKRTIEDIAHLQMPLLLPLPKDNTNLFLSYGNFYV
                     YRNGILYQFAENTPQKGFHKLLEGPDFSFGEFTILKTNQDKQLVYLDFEKETITYLSF
                     TTKLSKRYKLEGLSVSRKKVSLLVIDNTPFITDYYWVWKIVNGGNALKVEKPGNHLKM
                     FDEHFNKLQAFKKDFSRYRKYYSVVTRLDKLSTDYDDLFINELTLSSEKFYKMGDYEN
                     EEIGTKHVNLILKRIDAKMLGVIKLIKEEFQLSLSDAKNLAENTPCVLIENVSESKAL
                     ALKIKLEETGAVCYTETTQFVTKDGSTINLKDGVLVFTSSNKSIPQFFIPFILNQKLA
                     FATKYEFAGNSYFLPSDAKLTQIEAYDFFQKYVVKFIEEIFSHAS"
     gene            10041..>11901
                     /locus_tag="PLF48_03960"
     CDS             10041..>11901
                     /locus_tag="PLF48_03960"
                     /inference="COORDINATES: protein motif:HMM:NF039752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48513.1"
                     /translation="MHLRLIRSPKNKYPLKGILIKGNSPAEWLCEMEAMHIKPEYCDA
                     YALPNKDNRGLFACLLVFNKEMTIADIRKNARVQSIADKIFISEHTDIYPELTTYDIQ
                     KEFAVFPYFFHPETGLTELNETINWLYCLSAPTLHDAEILIPEDSHPVPHKITAYKAV
                     INPESWMSAITDAQTEEEWYKKLPFNMKKLLEGNQKEMERYMAYLSKNPEMALKHAIP
                     LDIMGTSRGKQMAKFIFKDDNWFNRLLDKLFTVKSSGKATKTANNRNTKEPGNYKWMP
                     LFIILLVVVAFAIAMMRSCNVSPAALNPPVVITEVAILKYALMLTGMFALVILFVYVF
                     SAIKERSTIPSGNSPKTGTATIPNIINWDQLDLKDKKEKTSFSFAPELSLLSRIILIF
                     VLCVSLSYLFLPLSVYAGWTNWFTLFAMGTMLYCTYKLLKTNETLLSSDSKKEETDEH
                     KAAVYNLLIYFLKAAVILSYLFTKGFFPFLGSLIGGIVLLLATTFIIIFLFNKKTDAD
                     GAGSNGGVVTLRNDIFDKITQQYEKLAQNYIDKKDFSRAAYIYLKLLKSPQRAADTLY
                     DGKQYLNAAHVYQHICKNKRRAAESFEAAKVYTAAIEIYQELNEPEKIGDLYML"
BASE COUNT         4078 a         2148 c         2256 g         3419 t
ORIGIN      
        1 tgtacaatat cttttctcgg acgcaaccct ttgaaaggac ggatagtaat catgaatgaa
       61 gtgttatatg tttttatatt ataatttata tatttcctag gtaatgcgtt tccagcacgc
      121 tgacgatagc tcctgccgtg tagcccaaaa aagccagcca gcctatccgt ttggcatacc
      181 acataaagtt tattttttcg atgcccatca ctgctactcc ggccgccgaa cctatgatta
      241 atatactgcc tcctgttccg gcacaatagg ctataaaatg ccaaaggtgg tgatcggttg
      301 ggaaggtata catactttgg gtggcagata ctaacggcac attatctatt acagctgata
      361 ataagccaat agtataaata ataatattat ggtttccaat gctattatcc aggtgaagag
      421 caagagattt taatatacct gaaacatcca gggcacttac agccgtgagt atgccgaaaa
      481 aaaacaagat actcggcgtg tctatttttg acatggcatt ataaacggaa taaccaaatt
      541 tgtcttccgg attcttttct ttgtgcagca aatcggtaat cacccacatg atgcccaaac
      601 ccaagagcat gcccatgaaa ggtggtaaat gtgtaacggt tttaaatatt ggtacagaaa
      661 ttagcgtgcc taaacccaat aaaaatacaa cccaacgcaa ccaaataggt tgatcggttt
      721 ctttattttc agatttagga gtttccactt cgcctttcat gataaaacta ataaccaaca
      781 agggcacgag taaactaaca gcactggcaa aaaagatttc tttaatgata gacatcgctg
      841 taatttgtcc tttaatccag agcatcgttg tcgtgatgtc gcccatggga ctccaagcgc
      901 cacccgagtt ggcggcaatt acaacaattc ccgcaaaaaa caggcgatct tcttgattgg
      961 ctattttttt gcgaagcaaa gagaccatca cgatggatgt ggtcaggttg tctaaaatgg
     1021 cagaaagaaa aaaggtaatc caggcaatta tccagagcaa ccgccttttg tctgtggtag
     1081 taattctcga aacaataagt tcaaaaccgt catgctggtc tataatttct acaatagtca
     1141 tcgctcccag cagaaaaagc aggatactgg agatttcggg taaaaactcg tgcataaatc
     1201 ggtggctacc gtcatccaca cccgaagcca atatccacac ggtccataat accacgccgg
     1261 ttatgagtgc agcccctgct ttatcaaacc cgagcttatg ttcaaatatg atagcgaagt
     1321 acccaagaca aaaaatcagt agcaatattg cttctatagt catgggttaa atatgcaaaa
     1381 aaaaagtcga ttttgcaagt aaagcaggcg tgttttattg ttgaaagcct gaaactacat
     1441 ctgccaaaca atcttcatta tagtgtttga gtatggtaga tgattttttg tagttcttag
     1501 aaggatgata aataaacaga caagaaccgc cttttatcag cgagataatt ttttcatttt
     1561 tttccaatgg caaagccgga cagccttggc ttctacccaa ttttccgttg gcgtttatga
     1621 aatcgtaact aacgtagtct gcaccgtgaa ttacgactcc tctttcgcgt gcatttgagt
     1681 tgatgccggg ctccatgccg tctagtttca gcgagaaacc attttttccg ccatacgttt
     1741 cacctgtaat ataaaacccc aagctgcttt gatatgattc agcccaattg gagaatttat
     1801 cggcataaat ttctccgcta tttcttccat gagcacacaa actcttcgct attatttttt
     1861 tagttcttag gttgattacg aataaacgct cagtggtgga gggcttgctg aaatctacga
     1921 tggttataat gtcctttttt tgtatttgcc ccttttcatc caaataagcg taaccactca
     1981 atgctgtttt catagcttca tcagaaacaa cagcaaaaca atctaaatag tcttgcaccg
     2041 tagtatttgg ctcggtttca gctactacaa ctgcagggtt ttgctcttgc ttaataacca
     2101 aatgattttc gtcgccgaat gattttgtat aattaagtgg gaaaaataat accgcccaca
     2161 tcaagaagat ggatttgcga accattaaat aaaatttttg ttgtttacgt gatgtaaaaa
     2221 cgactgatag gcgtgtttta gtattccaaa aataaccaat acaaggtttt tatcctcatt
     2281 ttcagtgagc ttaattctta aaaacctgtt aaacttcagc tttttaggaa aaatttaaat
     2341 tttcaaagta aaaacagatt gataataatc aataattgta acaaaattgc aaaatatttt
     2401 atcaaatgca ataaagttgc aaatattgcg ttatctttat taactaaaaa catgagtatg
     2461 aatacgaaac aaacaagaac gatggacgaa atagttttcg aaaatcgaaa caaggcttat
     2521 ggtgcttatg tgctgcgccg tgaagtaagt aaaaatgaaa aaattggatt tttggttaca
     2581 attctgttat tcatttcagc actttatatg ggtaagcttg cacaaggatt atttagcaat
     2641 ggctttgcca tggtgcacaa tgaaggtaaa acggtaactt ctcaacttgc tgatgttcat
     2701 accgaggaat atgtacgccc taaaccggaa ataaaatcag aaccaccaaa agaattaccc
     2761 aatacctcac ccgaacaaaa tgtaatccct gtaacccaac cgcagcaaaa tacacaggat
     2821 tttagagaaa tgaatgcagt agaagataac aaagtaaata cagataattt agccaaacaa
     2881 attgacctgg ttgataaaac agttggaacg gtcaccacac caaaagatcc ggattatgtg
     2941 gcagtggttt caacacctgc cgtagtaacc agcactccgg aacctgtaaa agttgtagct
     3001 gaaagaccca gaccagtggc ggaggttatg cccgcatttc ccggcggaca agccgcattg
     3061 ctggcttact tgaaaaagga aatgtcttac tattcatttt ctgataatga acaagacggc
     3121 aaaatcttta ttcgctttta tattgatttg gacggaagta taaaagaagc tactgtggtt
     3181 aaagatgatg tgggtgggta tttcggtaaa cgagctctta gtgtaattaa taaaatgccg
     3241 agatggtcgc ccggaaaaca aggcaaccaa gccgtgagag tattttatac tattcctatc
     3301 acttataaac aattaggcgg ttaatttggg tggttatgag tagctaattt ttttaatctt
     3361 gtttagcaaa ctttttgcat atttattctt cctaaaacag aagaatgagc aaaaccattt
     3421 tgataaccgg agcaacggca ggaattggca aagaaacagc aaaacaattg gctgcacaag
     3481 ggcatttgat ttacattacc ggacgagata aaagcaaatg cgaagaaacc ctgcaagtgc
     3541 ttaaacaaat taatccaaca gctgcgggag gctatttttt atctgatttt gctgatttga
     3601 gcagtgttcg aaaaatggca aatgaaatta ataatacact cccgcaatta gatgtgttaa
     3661 tcaataatgc aggtggtgtt tttgccaagc gcgaactaac atccgaaggc tatgaaaaaa
     3721 cattatgtgt caatcatttg tctccatttt tattgaccga tttattattg accttgttac
     3781 aaaaaagtaa agacggaaga attgtaactg tagcttcaga ttcccattat cgcggtgacc
     3841 tcgatttcaa tgatttgcat tttgaaaaaa actattttat tctgaaagcc tatgagcgca
     3901 gtaaattgtg caatgtatta ttttctaatc atttggctaa acaattagaa ggaacgaatg
     3961 ttagctcaaa cagtttacat ccagggcatg tggtttctga tattggtgca aaaacatcta
     4021 accctttttt caaatgggta tggaaaacgg ttaacaacct gacaggaata ccaacagcag
     4081 aaggagctaa aaccagtgtt tatttagcta gcgctgatga agtggcttcg gataacggtt
     4141 attattttga taaatgcaaa cccaaaacac catctaaatt ggcacaagag gaacaacttg
     4201 cagaacaact ttgggacata agcaaaaaaa tggttgggat ttgagttgta ataaacaata
     4261 aaacttcttt gcaaaaagat attgtgtata tttgtgtaaa aattacgcat ttatgtcgct
     4321 tcaacaaaat atcaaaattg ccgtagatgc cattgtcttt ggttatacgc aacatcagct
     4381 tcatgtgctt ttggtaaaac aaaaatacgg acatctgaaa aacaactggg tgatagccgg
     4441 aggttttgtt aaagatgatg aaacgctgac acaagccgtg aagcgtgaac taaaagaaga
     4501 agccggaatt actgttgatt acatggaaca actctacacc tttggagacg acattcacag
     4561 agatccgcga tttagagtaa tcaccgttgc ttattttgcc ttagtgaatc cgactaaact
     4621 taagctcgtt gccgacacag atgccgagga agcccgttgg tttccaatga atgaagtacc
     4681 aaaactaggt tttgatcata atgatatttt aatagttgcc ttaaaaaggc taaaagataa
     4741 attgacttat cagcccattg gttttgattt gttgccaaaa gaatttcctt tttcagattt
     4801 ggaaaacctg tattgttcaa ttttggaaac agaaattgac agacgtaatt ttagaaaaaa
     4861 agtgctgagt tttggactcg tggaggaaac tgaaaaatat gcaaataaaa aggaaggcag
     4921 accggcaaaa ttatatcggt ttaaccggca aaaatatcaa cagtttgtaa aagaaggcta
     4981 tcattttgaa ataaaataaa gatgcagaca caaaatgctt tttacgatac aatagttcat
     5041 ttgcgtttag aagaagaggc ggttgtattt acagacaaga ttagtataag tactctggaa
     5101 gaacgcctgc tgacctcatt tttgcaacaa gaatatgaaa aggaaaaaac aaactacccg
     5161 tttgaggctc ctgaatttca acccgaaacg gcactctggg cggcaaagat ggtatatttc
     5221 gccatacacc ttcatttctt ccggcaacaa gaagtcgcag ttgtaaaaaa catttttata
     5281 cccaatacca aagaaccaaa tgcagcacaa atgctgtcag ctgatattgt attgcgcttt
     5341 ttgttaccgt tatattcaga tattaaacaa gttgattttc aggatgtgat tttgcagggc
     5401 atagaggatt gtttaaaagc ctatcattac tcagcaatag gatcagattt agaaattcag
     5461 gagtcaactc ttgatgtatt gtttcaacaa ccttgcttca gacaacttta tctggacaga
     5521 gtaacacaat ataaatcaat cagccttgca aagctggaaa aaatagacga agggcttaaa
     5581 gaaaatttcg gcaattacag ttccggattt tggcagagaa cataatgcgg atttttcata
     5641 tattgccgta caaatacaaa aattgaaaat tatattatgg agttgacaga taaactaaat
     5701 gcagttctaa aagagataaa agaaactttt gtcagtaaag atgaaattat agacttatta
     5761 ggtattagcc tcttggcaag agagaatgct tttttatttg gccctccggg aacagcaaaa
     5821 agtgccatta tccgcaattt atctcagcat atagagcaag gaaaaaattt tgaatacctc
     5881 ctcacccgct ttacagaacc taatgaaata ttcggaccgt ttgatgtacg caaacttaaa
     5941 gaaggcgaat tactgactaa tacagaaggt atgttgcccg aagcgtctat ggtatttctg
     6001 gatgaattgt ttaatgccaa ttctgccata ctcaacagtt tgcttatggc actgaatgaa
     6061 cgcattttca gaagaggaaa ggaaacacgc aagctgccgg cacttatgtt tgtcggtgcc
     6121 agtaacttat tgcctgaaga cgaagccctg aatgcactac tcgatcgttt tctgatcagg
     6181 gtgcgatgtg ataatgtaga accggataaa ttgcaggaag tgttattagc aggttggaaa
     6241 ctggaacaaa ataccaccca acagtttaca acgattacag cagacgaaat cagaatactt
     6301 caacaggctt gtaagcaaat taatttgcag ccaataagat cagcatacat tgacctgatt
     6361 cataatttaa gaaataccgg cattaaagtg tcagacagga gagccgtaaa actgcaaagt
     6421 ttaatagcag caagtgcgct gatttgcaag cgcaaagaag ctgtcttatc tgatttgtgg
     6481 gtgttgaaat acatttggga tacagaagaa caagtagaaa tactggaagg gatggtcaat
     6541 gctatcattg aaaaagacaa gagcgaaaaa acccatccac aggcttttta caataaatcg
     6601 ccaaatgccg atgaagtaat gaaagaagta ctttttcttc aaaataaatg gaaagaagaa
     6661 ggactgagtc tggaagaaaa gaatgttgta aaagacaaac ttcgttatgt gcaatctcgc
     6721 tgtgactgga ttcatgacag agagcaaaaa gaatatatac aaaaggaaat cgataagcta
     6781 tggaaacaaa tactggaaga cgtatagttc agtatattat cgaaataccg gctacacgta
     6841 aagatgattt aggcagcatc cggcactggg ataatattaa gatggcatca gatgctgaca
     6901 gtatttggat aaaagatatt acaccccggc aaatagacac accggaaatc agaaaacttc
     6961 cgcaaaaaaa tatttacact attgaacaac agttgctatt taaactgaac ggattagtac
     7021 ccacaaaaaa gctgccacat ggtttattgt ggacacccat cgaaagggct ttaccaataa
     7081 aattgccaga ccatacaaat aattattacc aaccggaaaa acgtaagatc aaattgaaaa
     7141 cagtggataa ggaacacgaa cctgatgccc tactaattga aatgaatgaa ctggaacggt
     7201 acataaaaac cgcccctgct ataagattaa acaagataaa atggataata gtagaaggca
     7261 aagcattgct tatcggcaag ccgcttttac ccattaacgg tatttcattt tggtttaaaa
     7321 gcccattttt tttaccctgc gggatggact ttgaatacag cattttacaa tcatatttta
     7381 tgcaactgct cgatccactg aatgagaatt tcgtaatttg gttaagcaat cagtctatca
     7441 tccgcttctc taaatatgcg gtaaagccac taaatattgg atctttcaga ctaacacaag
     7501 cccaaaatga aaggataaaa tgacagagct gcataaatac tttgagcata gtaccgaata
     7561 tatctggaca tgggaggtgg atgaaatcag ccctcagcaa aatgtattta tcttggctgt
     7621 tccccatagt ttcacaatta tttatctcaa cgagcttgta gatattttag aatttttatc
     7681 gccacaaggt gttcccattt tgggggcttt gttattaagt gttttggcaa ccggaaataa
     7741 cgcagccgat gccctcagtg tagtcaactt acgtattcaa aagctacttg ctctctatcc
     7801 ggttgataca catatccgaa atgatgtgaa agacgcattt gaattattgc ataaaataaa
     7861 gaacctgcct ccggagtata aaacaaatga caaacgcaga ttggtgtttc aggttttgtt
     7921 cagagattgt cataataagc tatcagctac cagagcaaga agtctgatca aagaaatcaa
     7981 ccgaaatgaa tatagaatag atgaatacgc accaccattt gaattcgata tggccatctt
     8041 gaaacgcgat ttccaaacgc tttcaattca aaataaaaaa tttggcaaca cgcaaaatat
     8101 tatgcaggct attgctaaaa tgctggatga agagatgctt gcagaatact gggatgaaga
     8161 aatgatagac gaagaagaaa ttcaacccaa aaattttatt gaccagctaa gtatgcaccc
     8221 cgaaacattc agaattggtg ttttgataaa aagaatctgg ggaggattgc gcattccgct
     8281 tcatcaacaa ataccaagcg ggcagccttt gggcggaatt tctgacatga ccaataaagg
     8341 agattttgat cggctcttat tgtctgaatt tgcaaatgat gatgatgttt ttttatcgcg
     8401 tattgcaaat caggaagcac tttttataca gcgggaagta ccgcctcagc aagataaaat
     8461 tacccgttat ttactgatag atattacgct aaagaactgg ggtacaccaa aaattattgc
     8521 ttatgcttta agtcttgcta ttgccaaaca tccaaaaaac gaaattactt gtaagtattt
     8581 tgttttagga aacaagcata aagaaattta tttggaagac ttagacagtg tgatttttag
     8641 ttttaaacag ctttcatcaa aactggattt gacggatgga cttcaatcct tttttgaaga
     8701 agaacaaatt gatgatcatg ctgaaatttt tatcttatct tctccaaaca ccattttctc
     8761 tgcagctaat caacaagccc tccaaacata cagagcgtat tttcactatc taataacggc
     8821 agatgcaaat ggcgttatta atttgtacaa atacggcagt cgcaataaac gacatttaca
     8881 aaccatacaa cttcccttag aagaattgtg gaagaatgct cctgttaaga tttcaagaaa
     8941 gaaaagaact attgaagata tagctcactt gcaaatgcca ctattgttgc cattgccaaa
     9001 agacaatacc aacctgtttt taagctatgg caatttttat gtttatagaa acggcattct
     9061 ctatcagttt gctgaaaata cacctcaaaa aggatttcat aaattgttgg aaggaccgga
     9121 tttttctttt ggagaattca caatattgaa aacgaatcag gataaacaat tggtttattt
     9181 ggactttgaa aaagagacga taacctacct ttcatttacc acaaaattga gcaagaggta
     9241 taaattggaa ggactatctg tatcaagaaa aaaagtttct ctgcttgtaa ttgacaatac
     9301 acccttcatt acagattatt attgggtatg gaaaatagta aatggtggaa atgcgctaaa
     9361 agtggaaaag cccggaaacc atttgaaaat gttcgatgag catttcaata aattgcaggc
     9421 atttaaaaaa gactttagtc gctatcggaa atattattct gttgtaacgc gtttggataa
     9481 actttctaca gattatgatg atttatttat aaacgaactt acgctaagca gtgagaagtt
     9541 ttacaaaatg ggagattacg aaaacgaaga aatcggcaca aaacatgtaa atctcatttt
     9601 aaaaaggata gatgcaaaaa tgcttggagt aatcaaatta ataaaagaag aatttcaact
     9661 tagcctgtct gatgccaaaa atttagcaga aaacacacct tgtgtgttaa tagaaaatgt
     9721 tagtgaatca aaagcattag cattaaaaat taaactggaa gaaacaggtg ctgtctgtta
     9781 tacggaaact acacaatttg ttacaaaaga tggcagtacc atcaacttaa aagatggtgt
     9841 attggtcttt accagcagca ataaatcaat tcctcaattt tttattccat ttatcctcaa
     9901 ccagaaactg gcatttgcta ccaaatatga gtttgcgggt aattcttatt ttttaccctc
     9961 tgatgccaag cttacacaaa ttgaagcgta tgattttttt caaaaatatg tagttaaatt
    10021 tatagaagag attttcagtc atgcatctta gactcattcg ctcacccaaa aacaaatatc
    10081 cgctcaaggg tattttgatt aaaggcaact cgcctgcaga atggctgtgc gaaatggaag
    10141 caatgcatat taaaccggaa tattgcgatg cctacgcatt gccaaataaa gacaaccggg
    10201 gtttatttgc atgcttactt gtgtttaata aagaaatgac aattgcagat attcggaaaa
    10261 atgccagagt acaatccatt gctgataaaa ttttcatttc tgagcatacc gatatttatc
    10321 ctgaactgac tacatacgat atacaaaagg agtttgccgt atttccttat ttctttcatc
    10381 ccgaaaccgg attaacagaa ctgaatgaaa caataaattg gctttattgt ttatctgctc
    10441 caacacttca tgatgcggag attttaattc cggaagacag ccatccagtt ccgcataaaa
    10501 ttacggcata taaagcggtg attaatccag agtcatggat gtcagcaatt actgatgcgc
    10561 aaactgagga agagtggtat aagaagcttc cgtttaatat gaaaaaattg ctggaaggca
    10621 atcaaaaaga gatggaaaga tatatggctt atctgagtaa aaacccggaa atggcactta
    10681 aacatgcgat accgcttgat attatgggca cctccagggg aaaacaaatg gctaagttta
    10741 tctttaaaga cgataattgg ttcaacagat tattggataa actgtttact gtaaaatcat
    10801 ctggcaaagc tactaagaca gcaaacaacc ggaacacaaa agaaccggga aattataaat
    10861 ggatgccatt gtttattata cttttggttg tcgtagcttt tgccattgct atgatgcgtt
    10921 cttgcaatgt gtcacctgca gccttaaatc ctccggttgt aataactgaa gttgctatac
    10981 ttaaatatgc attgatgctc accgggatgt ttgcattggt cattttgttt gtgtatgttt
    11041 tttcagctat taaagaaagg tcaacaatac caagcggcaa ttcacctaaa acgggaactg
    11101 caactattcc aaatatcatc aactgggatc aactcgattt aaaagacaaa aaagaaaaaa
    11161 caagtttctc ttttgcacca gagctatcct tgctgtcccg aattatcctc atcttcgtac
    11221 tctgtgtatc cttatcctat ttatttctgc cactttccgt atatgcaggc tggacaaatt
    11281 ggttcaccct gtttgcaatg ggcacgatgt tgtattgtac ttacaaatta ctcaaaacaa
    11341 atgaaacctt gctttcttct gacagtaaaa aagaagagac tgatgaacat aaggctgcgg
    11401 tatataatct attaatttac tttttgaaag cagcagttat acttagctat ttgtttacta
    11461 aaggattttt tccattcttg ggctcactta tcggtggtat agtcttattg ctggctacta
    11521 ctttcataat tattttcttg ttcaataaaa agacggatgc ggatggtgca ggaagcaatg
    11581 gcggtgtagt aaccttgcgc aatgatattt ttgataaaat aacgcaacaa tatgaaaagc
    11641 tggcacaaaa ctatattgac aaaaaagact tttcaagagc agcctatatt tacttaaaac
    11701 tattaaaaag tcctcagcgg gctgcagata ctttatatga tggaaaacag tacctcaacg
    11761 cagcacatgt atatcagcat atttgtaaaa acaaacgaag agcagccgaa tcctttgaag
    11821 ccgctaaagt atatacagcg gcaattgaaa tttatcagga attgaatgaa cccgaaaaaa
    11881 tcggcgattt gtacatgctc a
//
LOCUS       DAOQII010000052        11791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2730_length_11791_cov_5.563906, whole genome shotgun sequence.
ACCESSION   DAOQII010000052 DAOQII010000000
VERSION     DAOQII010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_11791_cov_5.563906"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLF48_03965"
     CDS             complement(<1..476)
                     /locus_tag="PLF48_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48514.1"
                     /translation="MRNSGRHIIVTGCIVVCMALSFFSVTAQDKKTQLEAKRKKLEEE
                     IKFTQKLLQKTKANKDATMADLNALNEQIKLRQTLIEEVGVEINAYSAKINENVEHLK
                     TEEQQLTQLKSEYADAIVKTYKYQRFADKLMFVLNAESFAQAWRRINYLRKYAEYR"
     gene            complement(466..1329)
                     /locus_tag="PLF48_03970"
     CDS             complement(466..1329)
                     /locus_tag="PLF48_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNF48515.1"
                     /translation="MRKDVFLFRAGFDRHLLSIGLIFLVLFTVFLSGCKHRKEIAKTP
                     QKQDTLATVKPPMQPTPAKNIDPNVAKTLQLLQKNQLVFKELSARLKTKVKSPDLNQS
                     FTTNIRWKKGEKIWLSMSIIGIEGVRVLITKDSIKVMDRLNDRYILKPINYIKQKALI
                     DLSFTDIENLLLGQLLFTDTANTKFADNPANITLTSNSTRFLNTIIFNKPSQFLSSMF
                     VTDKINNQTLTSSYTNYQTQLNKQFSMERNLVVKSKGETFEMQATFQAIKAEQGLDYP
                     FPIKSGYKIEK"
     gene            complement(1329..3056)
                     /locus_tag="PLF48_03975"
     CDS             complement(1329..3056)
                     /locus_tag="PLF48_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF48516.1"
                     /translation="MLLSWQQPAFAKKKKKDKSTSEAGKKNDYALQSLFIDGTKAYLL
                     GNNEEAVAKFNEVLKKDKNNAAAYYQLSRIAFENGNAAKALSFINSAINIDPKNEYYF
                     MYQAEEKASLGDYEGAAKAYETLTKLYPRDYDNYFSLGYMLTKANKYREAIDAYNLLE
                     QKTGVSEELTFQKQPLFIKLGKIDEGAKDIEKLVKAYPDEPRYIGLIGELYEANKQYD
                     KAIEAYNRVFAVEPDNAEAMLALSEVYRKKGDEAKRLEYINKVFGNKNIDIDTKIYSF
                     IPFVERLQKDSSTESRDAVMKMADMIVAAHPDNIKAVTVRADALLNSNKKSEALAEYL
                     KAISMDEAPGTVWVQIYLLDAEMEQYDSLIAHTKLGISKNPKDVLGYFYNALGYQNKK
                     EYSPAFNTLQKGFDVMKSNKPDAFTYTPQLKLQMLISLGDVAFELKKYETSDSAYDAA
                     LEIDPNNATVLNNYAYYLSERNIKLEKAERMSKKSNLLVDNNSAFIDTYAWIMYKMKN
                     YKEALEWMEQAMQIPDSKERAELLSHYGDILYKVGRVDDAMQQWQKAIDKGGDKILLQ
                     DKIKNKKAD"
     gene            complement(3276..6644)
                     /locus_tag="PLF48_03980"
     CDS             complement(3276..6644)
                     /locus_tag="PLF48_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNF48517.1"
                     /translation="MKTVINQLLATDAFLSTESKDAKKLYDRSNIIDKWQFVGEFDNI
                     SGSGFEKDYGPLSNPSANAQFDNKIGAKVKWFTLPQGRIDKWMDLEYSFVIGNSIMYA
                     QTFVKSETEQDVVFRIGVSGSMKVWLNDALVGTENEERNTDLDVYNYKTHLTKGYNRI
                     LIQIGESEADNANFLLRITDDNGNAIQGLTISAEPQPYTKLKTVKSEQIPLFAETFFE
                     QKIQTNPKDIINYYLLAYTYLRNDKVYEARKILYKARELAPNSGLLSRELIEAYQREK
                     NQTDQSKEVENLKKIDPDNYLSIQMLISEAEDKENYDEMEKLVDKLERLYGIDERTMT
                     YRLSLASDRKKNEDIVKITEEGYKKYPDVYLFVKMKAVIEEEVNKNMRGAISVYEKYL
                     KNNYSEKARAALSDLYFKNGNPDKGVKMYEQMIDEKPYAVGYLADLANYYTQTQTYGK
                     AIDYANQCLEYAPYIGKFWSILGKVYEAKNNKNDAIENLKKANYYNPRGYENKKLLRK
                     LSGKEDIYDYFTHPDMDLIAGDKITVNVTQDDNAVILHDETQKIIYEEGGSEEKQILI
                     TKVLNKSGVDDWKEYQIGYNPYSQRIIIEESKIIKPNGNKIEAERDESYLVFTGLEVG
                     DIIVIIYRVENYASGKLANDFWEEYNFNGFYPRQHVKISYIIPNSKKFEHKEINMDLT
                     PQTKTIEGNSTMYTWELKDQPSLKPESYMPPLDDVGKILHISSLPDWNYIANAYADMS
                     TTKAKANYEVKELIATLFKDKTNLTELQKAKIIYEWILDNIHYSHVSFRQSGWVPQKA
                     STTINTKLGDCKDLATLFVSMAKEVGLKANLILINTHDNGERGLQLPSMDFNHCIAKV
                     NADGKDYYLELTYPQLSFSAQTSSIIGCQSLNIPNTATTSTNSIQNLLHPQSVPNKII
                     RESELTTDNNDIIIKRKNIHYGAYAASRREDFGEKSKEEREKDLQQSISKAFTKPVKV
                     TNAEFSDLKALTDSTVGTYYYTVKGGVTDFGGMKLFEIPWTDAESSPDFVSTDKRDYD
                     IYFWRYDYADAMTETMSISIPKGKTLVEVPKSVHFKYKSTTYDITYKVEGDKLIAERK
                     VINGEKIIPASEYDAFKEFYNKIIQEDHKQIGFK"
     gene            complement(6641..7027)
                     /locus_tag="PLF48_03985"
     CDS             complement(6641..7027)
                     /locus_tag="PLF48_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48518.1"
                     /translation="MKKLLFILCWNMFMFTTHQAFASNLDDAWIAFNNNDRAKSKTLF
                     TQSLSNPKEKAEAYLGLSFIAFKEGKSKDAFQNFKSFYGVSEDPFPYLYALGYSGILD
                     EMQGKMSDEARDLAKKMYKIQEQTVQ"
     gene            complement(7087..7521)
                     /gene="dut"
                     /locus_tag="PLF48_03990"
     CDS             complement(7087..7521)
                     /gene="dut"
                     /locus_tag="PLF48_03990"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNF48519.1"
                     /translation="MKIKIVNNSENPLPTYETSGSVGLDLRAHLQEPVMLKPLQRALI
                     PTGLHIELPDGYEAQVRPRSGLALKKGLTVLNSPGTIDSDYRGEIKIIMINLSNETTI
                     INTGERIAQMIIAKVEKAQLKEVDELNETERGEGGFGHTGIK"
     gene            complement(7554..9008)
                     /locus_tag="PLF48_03995"
     CDS             complement(7554..9008)
                     /locus_tag="PLF48_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF48520.1"
                     /translation="MSILRRLASDTAAYGISSILARLINFLFGFLIIKYITQAEFGTY
                     TNFYAYAGFILVVLTHGMETAYFRFVNKSESKEDAFTTSFFSVLLAVLFFVLITYVFQ
                     QSIATYIKEPALYVKLFVWMMVFDALSAIPFASLRKQGRPLLFAGLKILNILFFIFFN
                     VLFFILFPKFNFSFQTTKVSYIFIANILASVLTFLLLLKQFNQLHFRLDKALYKTMLR
                     YALPIMLVGFAGMINEVLDRIIMTRLLPFDDLTNKEQLGIYGFNYKFAMMVTMFLQAY
                     RYAAEPLFFKNADKADNKQMLANTMKYYVICVCVLFLSITLFMPLIQYGFIRYSPKST
                     AYFSGAGIVPVLLIANICLGMYFNISTWYKIKDKTYVGAIIATIGALITIVMNVVLVP
                     RYGYWGGAWATLGCYFSMVIIGFITEQKYYTIPYNTARILFYLFFSIATYLLFDFVLK
                     NSLPTLLLKTITAFILLCMYMAIVYFIEKKKIAV"
     gene            complement(9086..10510)
                     /gene="asnS"
                     /locus_tag="PLF48_04000"
     CDS             complement(9086..10510)
                     /gene="asnS"
                     /locus_tag="PLF48_04000"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNF48521.1"
                     /translation="MSKLYISDILTNVAIGTEITVRGWVRTKRSSKNVTFLNINDGTT
                     IHNIQAVVEEGVVSEEVLARINTGACIAASGTIIESIGNQKVELKVKTIEIYGEADPD
                     KFPIQPKRHSFEFLREKAHLRMRTATFSAIFRIRHAVAFAIHNFFNTRGFYYLHTPII
                     TGSDAEGAGEMFRVTTLELENLPKQENGHVDFSQDFFGKETNLTVSGQLEGELGAMAL
                     SKIYTFGPTFRAENSNTARHLAEFWMIEPEVAFNDLEDNMNLAEDLLQYVIQYVLANC
                     ADDIEFLDKRFTEEEKQKPQNERSEMGLLEKLKFVLGNKFERITYTEAIEILKNAKKK
                     FQFPVDWGTDLQSEHERYLVEKHFKKPVILIDYPEKIKAFYMKLNEDGKTVRAMDVLF
                     PGIGEIVGGSQREDDYEKLKNRALQFGITEESIWWYLETRQFGTCPHAGFGLGFERLI
                     LFLTGMSNIRDVIPFPRTPKNAEF"
     gene            10751..11215
                     /gene="lysM"
                     /locus_tag="PLF48_04005"
     CDS             10751..11215
                     /gene="lysM"
                     /locus_tag="PLF48_04005"
                     /inference="COORDINATES: protein motif:HMM:NF008399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNF48522.1"
                     /translation="MGLFSFIKGVGEKIFGHSETPEEKKQKVVDHLNKFGILPATVTV
                     DVQDKKVIFGGTVDSLDARKRIIATAGNIDGIEEVEDNIVVVIKEQVVPVQKAKEQEF
                     YVVQAGDNLSKIAKHVYGDANKYPLIFEANKPMLSHPDKIYPGQTLVIPEEA"
     gene            complement(11240..>11791)
                     /locus_tag="PLF48_04010"
     CDS             complement(11240..>11791)
                     /locus_tag="PLF48_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNF48523.1"
                     /translation="YERIPFLFSLHYKTDASTTIAHQEYISPVGDDDRVNFIEAFLQA
                     TSKPGKILVFNTLMEKGMLNKLATDFPQYADTLRERLDRIIDLEIPFKELYYYHPKQQ
                     GSFSLKAIGNALLGEDEFYQSAIKDGETAMAVYNELFYKEDATYIKKQLDALCYYCRK
                     DTYVLYRIFEALKKTGILTDSDL"
BASE COUNT         3419 a         2268 c         2073 g         4031 t
ORIGIN      
        1 ttgcggtact ccgcatattt gcgaagataa ttaatgcggc gccatgcctg ggcaaaactc
       61 tcggcattga gtacaaacat cagtttatct gcaaagcgct gatatttata agtcttcaca
      121 atagcatcag catattcgct ttttaattga gttaactgtt gttcttccgt tttcaggtgt
      181 tcaacatttt cattgatttt agccgaataa gcattgattt caacacccac ttcttctatc
      241 agcgtttgtc ttaattttat ttgttcattc aatgcattca aatcagccat cgtggcatct
      301 ttgttggctt tggttttttg caataatttt tgagtaaatt ttatttcctc ttccagtttc
      361 tttcgctttg cttctaattg tgttttttta tcttgtgccg taacggaaaa aaatgaaagt
      421 gccatgcata caacgatgca tccggttaca ataatatgtc ttccactatt tctcaatttt
      481 atagcctgat tttatgggaa atggataatc caatccttgt tctgctttta tcgcctgaaa
      541 agttgcctgc atctcgaatg tttctccttt gctttttaca accagattcc gctccataga
      601 aaattgcttg ttcagttgtg tttggtaatt ggtataagat gaagtcagtg tctgattgtt
      661 aatcttatcc gttacaaaca tggaagaaag aaattgcgat ggtttattaa aaataatagt
      721 attcagaaag cgggtactgt tagatgtcaa ggtgatattc gcagggttat ctgcaaattt
      781 ggtattcgca gtatccgtga acagaagctg ccccagcaat aaattttcga tatcggtgaa
      841 tgacaagtca atcaaagcct tttgtttgat ataattgatg ggtttcagaa tatagcggtc
      901 gttaagtctg tccattactt tgatactgtc tttagtgatg agtacacgta ctccttcgat
      961 gccaataata gacatagata gccatatctt ttcgcctttt ttccaacgaa tgttagtagt
     1021 gaaagattgg ttgaggtcgg gtgatttgac tttggtcttt aatctggcag ataattcttt
     1081 gaaaaccagt tggttttttt gtaatagttg cagagtttta gccacattag ggtcaatatt
     1141 tttggcagga gtaggctgca tcggtggttt cactgtggct agcgtatctt gtttttgtgg
     1201 tgttttggct atctccttgc ggtgtttgca accagaaaga aaaacagtaa acagtaccaa
     1261 aaaaatcaat ccgatagaaa gcaggtgtct atcaaaaccg gctcgaaaca aaaaaacatc
     1321 ctttctcatc agtctgcttt tttatttttg attttatctt gcaacagtat tttatctcca
     1381 cccttatcaa tagctttttg ccattgctgc attgcgtcat ccactctgcc tactttgtat
     1441 aagatatcgc catagtgcga cagcagttct gcacgctctt ttgaatccgg aatttgcatg
     1501 gcttgctcca tccactctaa agcttctttg tagttcttca ttttgtacat aatccaggca
     1561 tacgtatcaa taaatgctga attattatca acgagtaaat ttgatttctt actcatgcgt
     1621 tccgcttttt ccagttttat atttcgttcg gaaagatagt aggcatagtt gttcagtaca
     1681 gtagcattat tcgggtcaat ttctaatgca gcgtcataag cactgtcaga cgtttcatat
     1741 tttttcagtt caaatgccac atcacccaac gaaatcaaca tttgtaattt gagctgaggt
     1801 gtatatgtaa atgcatccgg cttattgctt ttcattacat caaatccttt ttgtaaggta
     1861 ttaaaagccg gactgtattc ttttttattt tgataaccca aggcattata gaaataccct
     1921 aacacatctt tcggattttt gctgataccc agtttagtat gagcaattaa actgtcatat
     1981 tgttccatct ctgcatccag cagataaatt tgcacccaca ccgtaccggg tgcttcatcc
     2041 atgcttattg ctttcagata ttcagccagc gcttcacttt tcttgttact gttaagtaat
     2101 gcatctgccc gtaccgttac agcttttata ttatccggat gagcagccac tatcatatct
     2161 gccattttca ttaccgcatc tcttgattct gttgatgaat ctttttgtaa tcgttctaca
     2221 aacggaatga atgaataaat cttagtatca atatcaatat ttttattgcc aaatactttg
     2281 ttgatatact ccaagcgttt ggcttcatcg cctttcttgc gatatacttc cgacagtgcc
     2341 agcatggctt ctgcattatc gggctctacc gcaaatactc ggttgtaggc ttctatggct
     2401 ttgtcgtatt gtttattagc ttcatacaac tctcctatca agccgatata acggggttca
     2461 tcgggatatg ctttaactaa tttctcaata tctttagctc cttcatctat cttacccaat
     2521 ttaataaata aaggttgctt ctgaaatgtc agttcctcgc tcactcctgt tttttgttcc
     2581 agtaaattat aagcatctat ggcttctctg tatttattgg ctttggtgag catataaccc
     2641 agactgaagt agttgtcgta atcgcgtggg tagagtttcg taagtgtttc ataagctttg
     2701 gctgcgcctt cgtaatctcc taatgaggct ttttcttccg cctgatacat aaagtaatat
     2761 tcattcttag gatcaatatt tattgcgcta ttgataaagc tgagtgcctt ggcagcatta
     2821 ccgttttcaa aggctatacg tgataactga taatatgctg ctgcattatt tttatctttt
     2881 ttcagtactt cattaaattt agcgacagcc tcttcattgt ttcctaataa ataggcttta
     2941 gttccatcta tgaacagtga ttgaagggca tagtcatttt tttttccggc ttcagaagtc
     3001 gatttatctt ttttcttttt tttggcaaat gcaggttgtt gccaactgag aagcatgcac
     3061 aataaaatga ataaagagag ttgtttacca aataaaagat gttgtctcat atcgtgtgtt
     3121 aaaatagcaa ttaatttaag aatctattgc gaaaaacgta ctaaaggctt gaaaggttca
     3181 attacaagtc gcattcttaa cattatttag caggcttcag acattaaaaa aagtgcatac
     3241 ccggaaagta tgcactctgc cattagtgat gaaatttatt taaaccctat ttgtttatga
     3301 tcttcctgga taattttatt gtaaaattct ttaaatgcat catattctga tgcaggaatt
     3361 attttctcac cattaatcac ctttcgttct gctatcagtt tatcgccttc cactttatag
     3421 gtgatatcat atgtggtaga tttgtattta aaatggacac ttttgggtac ttcaaccagt
     3481 gtttttcctt taggtatgct aatagacatg gtctctgtca tcgcatctgc ataatcatat
     3541 ctccagaaat aaatatcata atcgcgctta tccgtggata cgaaatctgg agaggattct
     3601 gcatctgtcc agggaatttc aaataatttc attccgccga aatctgttac gccacctttt
     3661 accgtgtagt aataagttcc aacggtactg tctgttaatg ctttcaaatc actaaactcg
     3721 gcattagtaa ctttcacggg tttagtgaat gctttgctga tggattgctg cagatctttt
     3781 tctcgttcct ctttactttt ctctccaaaa tcttcacgtc tgctggcagc atatgcaccg
     3841 taatgtatat tttttcgttt gatgatgatg tcgttattat ctgttgtaag ctcagactcc
     3901 ctgataattt tgtttggtac gctttgcgga tgcaagaggt tctgaatcga atttgttgaa
     3961 gttgtagcag tattgggtat attcaaagat tgacaaccaa tgatgctgga tgtttgtgca
     4021 ctaaacgaaa gctgcgggta agttaattcc aaataataat ctttaccatc agcatttact
     4081 tttgcaatac aatggttaaa atccatggaa ggaagttgca accctctttc gccgttatca
     4141 tgtgtattaa tgagaattaa atttgctttt aagccgactt ctttagccat agaaacaaac
     4201 aatgtagcca aatccttaca gtcgcctaat ttggtgttta tggttgtaga cgctttttgt
     4261 ggcacccatc cactctgacg gaaagaaaca tggctatagt ggatattatc taatatccat
     4321 tcataaataa tttttgcttt ttgcaattca gtaaggttgg ttttatcctt aaacaaagta
     4381 gcaatcagtt cctttacttc ataattagct tttgctttgg tagtcgacat atctgcatag
     4441 gcattggcga tataattcca gtcgggaaga gaagaaatgt gtaatatctt cccaacatca
     4501 tctaaaggag gcatgtaaga ttcaggtttc agcgatggct ggtctttgag ttcccaggta
     4561 tacatggttg aattaccttc tatcgtttta gtttgaggag taagatccat gtttatctcc
     4621 ttgtgttcaa atttcttact gttcggaatg atataagaaa tctttacatg ctgacgagga
     4681 taaaatccgt tgaagttata ttcttcccaa aaatcattag caagtttgcc gctggcataa
     4741 ttttcaacgc ggtaaataat gacaataata tcacccactt ccaagcctgt aaatacaaga
     4801 taggattcat ccctttctgc ttctatcttg tttccgttcg gttttattat cttagactct
     4861 tcaattataa tacgttgtga gtatggatta tatcctattt gatattcttt ccagtcatcc
     4921 acacctgatt tatttaatac tttggtgatg aggatttgtt tttcttcgga tccgccttct
     4981 tcataaatta ttttctgtgt ttcatcgtgt aaaatgacgg cattatcatc ttgcgtaaca
     5041 tttacggtta ttttatctcc ggctattaaa tccatatccg ggtgagtaaa ataatcatag
     5101 atatcctctt tgccactcaa tttgcgcaat agtttcttat tctcatagcc acgtggattg
     5161 tagtagtttg ctttttttag attttcaatt gcatcatttt tgttgttttt agcctcgtat
     5221 actttcccaa gaattgacca gaattttccg atatatgggg catattccaa gcactgattg
     5281 gcatagtcta ttgctttgcc atacgtctga gtttgtgtat aataatttgc caaatcagct
     5341 aaatatccta cggcgtaagg cttttcatct atcatttgtt catacatttt tacgccttta
     5401 tccgggttgc cgtttttaaa ataaaggtct gacagggctg cacgggcttt ttctgaataa
     5461 ttgtttttga gatatttttc atacacacta atggcaccac gcatgttttt atttacctct
     5521 tcttctatga cggctttcat ttttacaaac aagtacacat caggatattt cttatatcct
     5581 tcttctgtaa tttttacaat atcttcattc ttctttctgt cagatgccaa tgataatcgg
     5641 taggtcattg tacgttcatc aatgccatat agtcgttcca acttatctac aagcttttcc
     5701 atttcatcat aattttcctt atcctcagct tcagaaataa gcatttgaat ggataaataa
     5761 ttatccggat cgattttttt caggttttca acttctttag attgatccgt ttgatttttt
     5821 tcgcgttgat atgcttcaat caattctcgg ctaagcaagc cactgttagg tgccagctct
     5881 cgtgctttat acaaaatttt acgagcttca tacaccttgt catttctcaa atatgtatat
     5941 gccaacaaat agtaattgat gatatctttt gggttggtct gaattttttg ttcaaagaat
     6001 gtttcggcaa atagtggtat ctgttctgat ttaaccgttt ttaatttagt gtaaggttgt
     6061 ggctctgcac ttatggttaa accttgaatg gcattaccat tatcgtccgt aattcgcaaa
     6121 agaaaatttg cgttatcggc ttcgctttca ccaatttgaa ttaaaatacg attatagcct
     6181 ttggtgagat gcgttttata attatacaca tccaaatcgg tattgcgttc ttcattttca
     6241 gtacccacta aggcatcatt aagccaaact ttcattgaac ctgaaacccc tatgcgaaat
     6301 acaacatctt gttcggtttc actttttaca aaagtctgtg catacataat ggagtttcca
     6361 ataacaaaag aatattcaag atccatccat ttgtcaatac gaccttgtgg gagggtaaac
     6421 cattttactt ttgctcctat tttattatca aactgtgcat tggctgatgg gttggaaagc
     6481 ggaccataat ctttctcgaa acctgaaccg gatatattgt caaattcgcc tacgaactgc
     6541 cacttgtcaa ttatattaga ccgatcatat aattttttag catccttact ctcggtagat
     6601 aaaaatgcat ctgttgccag caattgattt attactgttt tcattgtacc gtttgctctt
     6661 ggatcttata catcttttta gctaaatctc ttgcctcatc tgacatttta ccttgcattt
     6721 catccagtat gccggagtag cccaatgcat acaaataagg aaagggatct tctgaaacac
     6781 cataaaatga tttgaagttt tgaaaagcat ctttactttt tccttcttta aatgcaataa
     6841 aggacaaacc taaatatgcc tccgccttct ctttgggatt agaaagtgat tgtgtaaaaa
     6901 gggttttgct ttttgcccgg tcgttgttat tgaatgcaat ccatgcatcg tccagatttg
     6961 atgcaaaggc ttggtgagtt gtaaacataa acatgttcca acaaaggata aaaagtaatt
     7021 ttttcatttt aaaagtgaat atttaatgaa aggtaattac tttccgataa ataaaaaaat
     7081 atttttttat ttaattcccg tatgcccgaa accgccttcc cctcgttcgg tttcattgag
     7141 ttcatccact tctttgagtt gggctttttc cacttttgct ataatcattt gagcaatgcg
     7201 ttctccggta ttaatgatcg tggtttcatt agagagattg atcataatga tttttatttc
     7261 tccccggtaa tctgaatcaa tagtgcctgg ggagtttagc acggtaagtc ctttcttaag
     7321 tgccaaacca ctacgcggac gcacctgggc ttcatacccg tctggcagct cgatatgtaa
     7381 gccggtaggt attaaagcac gttgcagtgg tttcaacatc acgggttctt gtaaatgcgc
     7441 tcttaaatct aaacctacag agccactggt ttcatatgtt ggtaaggggt tttccgaatt
     7501 attgacaatc ttgattttca tactccaaat ataacacttt ggaatgtact ttatcaaaca
     7561 gcaatttttt tcttctcaat aaaatagacg attgccatgt acatgcaaag taagataaat
     7621 gcagtgatgg tttttaataa caaagtaggc aaactgtttt tcaacacaaa atcaaaaagc
     7681 agataggtgg caatggagaa gaaaagataa aacagtatgc gtgctgtatt ataagggatc
     7741 gtgtaatatt tctgttccgt tataaacccg attatcacca tactgaaata acagcctaag
     7801 gttgcccacg cacctcccca atagccatat ctaggcacta aaaccacatt catcactatc
     7861 gttattaacg caccaatggt agcaataatt gcgcccacat atgttttgtc ttttatcttg
     7921 taccacgtgg agatattaaa atacataccc agacaaatat tggcaatgag caatacaggc
     7981 actattcctg cacctgaaaa gtaggctgtt gattttgggg aatagcggat aaacccatat
     8041 tgtatcaatg gcataaataa agtaatggat aaaaagagga cgcacacaca aatgacataa
     8101 tatttcatgg tattggccag catttgctta ttgtctgctt tgtctgcatt tttaaaaaac
     8161 aagggctctg cggcatagcg ataggcttgt aaaaacatgg taaccatcat ggcaaactta
     8221 taattgaagc cataaatacc caactgttct ttattagtca aatcatcaaa aggcaataaa
     8281 cgtgtcatga tgatacggtc taaaacctca ttgatcatcc cggcaaaacc taccagcata
     8341 atgggcaatg cgtatctgag cattgttttg tataatgcct tatccaatcg gaaatgtagc
     8401 tgattgaatt gtttgagcaa cagtaaaaac gtaagcacac tggctaaaat attagcaata
     8461 aaaatgtaag atacttttgt ggtctgaaaa gagaaattga atttcggaaa caagataaaa
     8521 aacaacacgt tgaagaaaat aaagaacaaa atattcagga ttttcagacc ggcaaataaa
     8581 agcggacgac cttgttttcg cagtgacgca aatggaatag ccgacaaagc atcaaacacc
     8641 atcatccata caaaaagctt tacatagagt gccggttctt ttatataagt tgctatagat
     8701 tgctgaaaaa cataagtaat taagacaaaa aacaagaccg ccagaagcac ggaaaagaag
     8761 gaagtggtga atgcatcttc tttcgattcg gatttattga caaaccggaa ataagccgtc
     8821 tccatgccat gggtaagcac cactaaaata aatccggcat aagcataaaa attggtgtag
     8881 gtaccaaact ccgcttgtgt gatatatttt attatcagaa atccgaacag gaaattaatc
     8941 agccgcgcta agatgctgct gatgccataa gctgctgtat cggatgctaa tctgcgaagt
     9001 atgctcaaac aaaaaaattt gtgctaaaaa tacggaaaat gaaatttgct aagcgcagaa
     9061 attataaccc gcatctcatg tgtgcttaaa actcagcatt cttaggtgtt ctcgggaatg
     9121 gaattacatc acggatatta ctcatgccgg ttaaaaacaa gattaaccgc tcgaagccca
     9181 aaccaaatcc tgcatgcgga caagtaccaa attgccttgt ttccagatac caccagatgc
     9241 tttcttccgt tataccaaac tgcagtgcgc ggtttttcag tttttcataa tcatcctcac
     9301 gctgagaacc ccctacaatt tcgccaatac ccgggaacaa cacgtccatg gcacgcaccg
     9361 ttttgccatc ttcattgagc ttcatataaa aagcttttat tttttccggg taatcaataa
     9421 gaattaccgg ttttttaaaa tgcttttcta ctaagtagcg ttcgtgttca ctttgtaaat
     9481 ccgtacccca gtcaaccgga aactgaaatt tctttttcgc attttttaaa atttcaatgg
     9541 cttcggtgta agtgattcgc tcaaacttgt tacccaatac aaattttaat ttttccaata
     9601 atcccatctc gctgcgctca ttctgtggct tttgtttttc ttcttcggta aatcgtttat
     9661 ctaaaaactc aatatcatct gcacaattag ctaacacgta ttgaatgaca tattgcagta
     9721 agtcttcagc caaattcata ttatcttcca aatcattgaa agccacttca ggctctatca
     9781 tccagaattc tgcaagatgg cgtgcagtat tggagttttc tgcccgaaaa gtaggtccga
     9841 aagtataaat tttagaaagt gccatggctc ccagttcgcc ttccaactga ccgcttaccg
     9901 tcaggttggt ttctttgcca aagaaatcct ggctaaaatc cacgtgtcca ttttcttgtt
     9961 taggcaggtt ttccaattcg agtgttgtta cccgaaacat ttcaccggct ccttctgcat
    10021 ccgaacctgt aataatgggc gtatgcaaat aataaaaccc acgtgtatta aagaaattat
    10081 gtatcgcaaa agcaacggca tgacgaatgc ggaagatggc actaaaagtt gcagtgcgca
    10141 tacgcaagtg tgctttttcg cgcaaaaatt caaaagaatg tcttttaggc tggatgggaa
    10201 atttatccgg atcagcttct ccataaattt caatagtttt tacctttagt tccacttttt
    10261 ggttgcctat cgactctata attgttccgg atgctgcaat acacgcaccg gtgtttatac
    10321 gtgccaacac ctcttcactc acaactcctt cttccaccac agcctgaata ttatgaatgg
    10381 tagtgccatc gttaatattg agaaaggtga catttttgct gctgcgtttg gtacgcaccc
    10441 agcctctcac tgtaatttcg gtaccaatgg cgacatttgt caaaatatca gaaatataca
    10501 acttgctcat tttaaaaaaa ttgagtggca aaaatacgta ttttaatgtt tttgaaagtt
    10561 taagaaatat gcctgtttat gaaaagatta atcttattaa gctttatata gtttatgatt
    10621 attaagtata tttacaatac acaatcattc aatattgttg atataaaaat tatattttgg
    10681 aactttgctg gcataaattt tgttattttt gatgtaaatt tttattcatt aattaaacaa
    10741 actaaaaaaa atgggcttat tttcatttat taaaggtgta ggcgagaaaa tcttcggaca
    10801 cagtgaaact cctgaagaga aaaaacaaaa ggtggttgac cacttaaaca aattcggtat
    10861 tttacctgca acggtaactg tagatgtaca agacaagaaa gttatctttg gaggtacagt
    10921 agacagctta gatgcccgca aacgcattat tgctacggca ggaaacatag acgggataga
    10981 agaagtagaa gacaatattg ttgttgttat caaagagcaa gttgttcctg ttcaaaaagc
    11041 gaaagagcag gagttttatg tagtgcaagc cggagacaat ttgtcaaaaa ttgcaaaaca
    11101 tgtgtacggt gatgccaata aatatccgtt gatatttgaa gccaacaagc ctatgctttc
    11161 ccaccctgac aaaatttatc ctggacaaac gctggttatt ccagaagaag cttaatcagt
    11221 acagtataat tcaatgttat taaaggtcgg aatccgtaag gattccggtt ttttttagtg
    11281 cctcaaaaat gcggtataac acataggtgt ctttgcggca ataataacaa agagcatcaa
    11341 gttgcttttt tatataggtt gcgtcttcct tataaaaaag ttcattatac acagccatag
    11401 ccgtttcgcc gtctttgata gccgattggt aaaactcatc ttcacccagt aaggcatttc
    11461 ctatggcttt taatgaaaaa cttccttgct gtttcgggtg ataataatac aattctttaa
    11521 acgggatttc caaatctata atcctgtcta aacgctctct caatgtatct gcatactgcg
    11581 gaaaatcagt agcgagttta ttcagcatgc ctttttccat cagcgtatta aacactaaaa
    11641 ttttacctgg cttacttgtt gcctgaagaa atgcttcaat aaaatttacc cggtcatcat
    11701 cccctaccgg agaaatatat tcctgatgtg ctatcgtggt tgatgcatcc gtcttataat
    11761 gcaatgaaaa taaaaacgga atacgctcat a
//
LOCUS       DAOQII010000053        11786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2733_length_11786_cov_6.559458, whole genome shotgun sequence.
ACCESSION   DAOQII010000053 DAOQII010000000
VERSION     DAOQII010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_11786_cov_6.559458"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLF48_04015"
     CDS             complement(<1..326)
                     /locus_tag="PLF48_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48524.1"
                     /translation="MIKSVLSILLICLFLNAFSDVTSYHIGNQNEFIAVRNELYKNEN
                     YPDYIKAYPEIVHRLSSQKNTFELSFHFALLTESLFQLRDIATMQQLVTVVLPFTKKY
                     NANAYHS"
     gene            391..1452
                     /gene="leuB"
                     /locus_tag="PLF48_04020"
     CDS             391..1452
                     /gene="leuB"
                     /locus_tag="PLF48_04020"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNF48525.1"
                     /translation="MKKKIAVLPGDGIGPEIIAEAVKVLKAVEKKYNHEFDYHYGLIG
                     AHAIDETGNPYPEATHELCMQSDAILFGAIGDPKYDNDPTAKIRPEQGLLKMRSALGL
                     YANIRPVKSYDALLDASPLKNDRIKDVDFVVVRELISGIYFGKPRGRSEDQNIAYDTC
                     VYTRAEIVRITKTAFDFARNRKKKVTLLDKANVLATSRLWREVVTEYAKTQPDIILEC
                     NYIDSAAMEVITKPSKFDVILTENLFGDIISDEASVIAGSLGILPSASMGEKTALYEP
                     IHGSYPQAKGKNIANPMATVLSAAMLLEYSFGMHEESRAIINAVDRAMNENIVTEDIN
                     KGKYTTSEVGDKIAEFVMG"
     gene            complement(1473..3014)
                     /locus_tag="PLF48_04025"
     CDS             complement(1473..3014)
                     /locus_tag="PLF48_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNF48526.1"
                     /translation="MAKTINKGKKQKDWFRLKRYPHVGLQLEPKDRAWIEPYLKDKDA
                     ISKHAFYPFIHRQLKVRKFRKEICHDGTRSKLRKPSTKEREIYFSNHLDSNVFSYYSE
                     ILSTAYESEIRDLGISSSITAYRKIKLCPEKEKSRNKCNVDFAHDIFRFIKTNKEKDL
                     VAITFDIKSFFDNLDHKLLKKYWKRIIKSGADLPEDHYNVFSNITKFSYIEEEDLFEN
                     FKNQIIVERKPKSFKEIKIKKKNYLRNKRAVAYCSKDNIEEIRNLGLIKTNKRIKGNK
                     DYLLRVKGIPQGSPISATLANVYMLDFDKEAHDMLKNIGGVYQRYSDDMVAICPIEFE
                     QEIIDHFLTSIINYKLEIQKSKTKVYNFKYDKITERHYCFEKNLNTNRLQDNTLFEYL
                     GFQFDGYHTLIKNSSISNYYRKMKRTFARSRFYTFHNRTATKGQVFKTRLYKKFTFVG
                     AERRRIYQRHPSKSDLFILSNKYDWGNFITYAKLAEKTIIDNKIGGQLKRHWRKFHEL
                     MKSIN"
     gene            complement(3037..3330)
                     /locus_tag="PLF48_04030"
     CDS             complement(3037..3330)
                     /locus_tag="PLF48_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48527.1"
                     /translation="MRKVSGLLNSTFYHPQLSKTYVAYGAGIPIMQGQSQESSSMLRI
                     ICTVLIDFIVFIIMDKVTNRILPEREIGATFNRVLCNACTIPLMKIDVKACPV"
     gene            3504..4628
                     /locus_tag="PLF48_04035"
     CDS             3504..4628
                     /locus_tag="PLF48_04035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF48528.1"
                     /translation="MKIALLGPAYPYRGGIANFSERLAHEFMQQGDEVHLYTFKMMYP
                     NFLFPGKTQYAETPIPFPLHVSRRINAINPLNWWRVAKTIRKENYDILLTAYSIPFLG
                     PSLGTISRKAKAENTKTISIIHNLKPHEKRAGDDALSAYFLNSFDAHLALSKKVEEDI
                     LERDKKHPVLFYPHPLYDTFGKLLDKKEAMHLLNLSTEYKYILFFGLIRDYKGLDILL
                     EAMGDQRLKDKKIKLIVAGEFYGGEEKYRQIIQDKKIEDRVLLYNHFIPDNEVNRYFS
                     AADLVVQPYKHATQSGITQIAYHFSKPMIVTNVGGLPETVPHNKVGLVCEPNAESLAN
                     AILTFYTENKEKTFIANMDAEKSKYSWKGLVEKIKRVKNI"
     gene            4717..5739
                     /locus_tag="PLF48_04040"
     CDS             4717..5739
                     /locus_tag="PLF48_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNF48529.1"
                     /translation="MIIRSKAPLRLGLAGGGTDVSPYSDIFGGAILNATISLYAYATI
                     KPTSDGKIRIHAIDRNEKIELLSTTELPINGQLDLIKGVYNRIVKDYAHQPLSFDIST
                     YVDAPPGSGLGSSSTLVVTIVGAFVEWLKLPLGEYDIAHLAYSIEREDLNMQGGKQDQ
                     YAATFGGVNYMEFYAGDKVIVNPLRIKSKFLHELEHNLVLFYTQTSRLSSDIIKEQQE
                     NVHAKNENAIDAMHHLKQQSIQMKEAILKGEMDNIGKILHDGWLHKKEMAKGISNAEI
                     DAIYSAALSAGATGGKISGAGGGGFMIFYAPYIHKYKLTDMLNTLGGKVMAYNLVTEG
                     LTTWTV"
     gene            complement(5742..6047)
                     /locus_tag="PLF48_04045"
     CDS             complement(5742..6047)
                     /locus_tag="PLF48_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48530.1"
                     /translation="MKKTSLFLLTILLAFACSKKNTPTKTEVKENIPLESAPSETYLA
                     GKTTYATKCGKCHELFSPQKGNIDFWNIWVNKMAPKAKLTETEKNNVLAYLATDCLK"
     gene            complement(6054..7160)
                     /locus_tag="PLF48_04050"
     CDS             complement(6054..7160)
                     /locus_tag="PLF48_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF48531.1"
                     /translation="MHFSRFVRMLSALVLLNACTPTEVVQPFYSSEQDFINDTTTLTR
                     LELVGKKIFFDKNLSNPVGISCASCHAPLTGFSDPEHSAFSKGVLGLLGNRNSPSISY
                     LAFSPQQMYFDNIDSTWVGGFFHDGRASSLEKQLLEPVFSHIEMNMNNIAAVIDRINR
                     SAYRADFVSIFGEAVFSDSLQFIQSFQKAVVAFEKSNQVSPFTSKFDYYLKGKVQLTR
                     QEMRGLILFNDPEKGNCSACHPSSADEISGKVLFTDFTYDNIGLPPNPNQSLIPDFGL
                     GITKNSSRENGKFKVPTLRNIEKTAPYFHNGVIQTLEDAIRFYSERDIVGKFNSPEVV
                     EDINKDELGNLKLTTEEINDIKAFLLTLTDGYQP"
     gene            complement(7150..7965)
                     /locus_tag="PLF48_04055"
     CDS             complement(7150..7965)
                     /locus_tag="PLF48_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48532.1"
                     /translation="MKLKFTFLSLFFLSIASYANDRFFTYIYQSNVLEKKQKEIEVWN
                     TFRFKKETFFRAFEPRIEFEIGIGKNTQTAFYLNIGHSATAIPNGSEPELEKETEFSF
                     SNEWKHQFSNPVAQKLGSAIYGELTLGLNEIELEGKLILDKQVKKWLFVHNTVFEYEI
                     EKEPAYANGNLKMKRNHEFKLDFNFGVCYFLKKGFTVGLEAVQKNVFTPTEKLEHSAM
                     YFGPNASWRMDNFWITATILPQIAGFKGNAYDRKLNLTEFERLQTRLIFSYAF"
     gene            complement(8073..8957)
                     /gene="ygiD"
                     /locus_tag="PLF48_04060"
     CDS             complement(8073..8957)
                     /gene="ygiD"
                     /locus_tag="PLF48_04060"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNF48533.1"
                     /translation="MMRKTFLQLLSAFSLINSTMKAKELVGFATQLNNSEKMPLLFLG
                     HGSPMNAITVNEFTLGFEQIATTFEKPQAVLCISAHWETKGTAVTAMEFPPTIHDFGG
                     FPEELYQVQYPAHGQPALANTIQQHVSRTKIGLDEKWGLDHGAWSVLKHLYPKADVPV
                     LQLSLDYIQSPQYHYDLAKQLAFLRNKGVLILGSGNIVHNLGRIAWNKMEEDNYAYDW
                     AREAQTDVNRFISAKNHQPLIHYHTLGQAFNLAIPTPEHYLPLLYILGLQETNETAYI
                     FNDKAVAGSLTMTSVKIG"
     gene            complement(9080..9481)
                     /locus_tag="PLF48_04065"
     CDS             complement(9080..9481)
                     /locus_tag="PLF48_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNF48534.1"
                     /translation="MKKNYDIGLLILRVSLGALMLFHGAGKLQSGVSMIGDMLTDKGL
                     PAFIAYGVIIGEVIAPVLMIIGFRTRIAAFVFAFNMVVAVAMAHSGDVFKLTEHGSWA
                     LELQGLYFFGGLALFFTGGGKFGVSSKNNWD"
     gene            10066..10932
                     /locus_tag="PLF48_04070"
     CDS             10066..10932
                     /locus_tag="PLF48_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48535.1"
                     /translation="MAITNINGVPYLEDYDPDKPLGKQLYEWGERFGTSTGNNTQTSG
                     TTRNFFAQSPNRNMHLSVDEIVTAGLYRKTYDQDNTRVYMVNSKLRFNNERAFTLPEV
                     LIKAKALEDRFIKEFDGFELILFANSDGAVILQHFEYATPKPNQLILFLEINDKTNYF
                     GSVMRVKLGERIITSKLPFTQIYEFSEAHQVKISKDEIIDLINDGNLKNKFRYILRVL
                     NKISNPVSYISGIAFSKMGDVFKNTIPDFLEELKIAENRWNPDAQGLLSFIWKQRIRA
                     YGTTKYKGKSKT"
     gene            10847..>11786
                     /locus_tag="PLF48_04075"
     CDS             10847..>11786
                     /locus_tag="PLF48_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48536.1"
                     /translation="MPRGYYPLFGSRGLELMVQQSIRAKVKPDNHFKLVQKTVDDMFD
                     KIESRIDAFYKFITQYFPNSLNRKFHTILKTLNTKLKDARKVFNDSDWLSKLLELGTQ
                     MFNAYLCGIINGFIDAINGVFQLIGLVCYAIAALNKGASDILFAASMLVEVFENIFEA
                     IIEFDLIGFLWKCVKLPFKFVVGIYKFLKQITETDLKKLLKKLDTSLVKIAYVCGNIV
                     GFIIQLLLEALCTGGVAAVEDLIQTLFKFLRNPAESIANGIKATINISKEFFYNLIYK
                     LSKFFESLKSKAAYKAIDEWVEQLLAAIKKELGISDE"
BASE COUNT         3831 a         2107 c         2182 g         3666 t
ORIGIN      
        1 gaatgatatg catttgcatt gtatttttta gtgaaaggca agacaactgt taccagttgc
       61 tgcatggtgg caatatcacg caactggaac aaactttctg tcagcaaagc aaaatgaaaa
      121 gaaagttcaa aggtgttttt ttgcgaagaa agccgatgta caatttccgg ataagcctta
      181 atataatccg gatagttttc atttttgtac aactcatttc taactgcaat aaattcattt
      241 tgatttccaa tatgataaga agtaacgtcc gaaaacgcat tcagaaaaag acaaataagc
      301 aatatactta aaacagactt tatcactcat tataaattta ataatactcg aaagataatg
      361 ccctattttt tatctgctta tttttataaa atgaagaaaa aaatagcagt tttacccggc
      421 gatggtattg gtccggaaat tattgcggaa gcagtaaagg ttttgaaagc ggttgaaaaa
      481 aaatacaacc acgagtttga ctaccattac ggattaatag gtgcacatgc tattgatgaa
      541 accggaaatc cttatccgga agccacgcat gagttgtgta tgcaaagcga tgccatttta
      601 tttggggcta tcggcgaccc gaaatacgac aacgacccga cggcaaaaat tcgtccggag
      661 caaggcttat tgaaaatgcg aagtgccttg ggcttatatg ccaatattcg tccggtgaaa
      721 agctatgacg cacttttgga tgcatctccg ctaaaaaacg accgcataaa agacgtggac
      781 tttgttgtag tgcgcgagtt gatatcgggc atttatttcg gaaaaccacg tggcaggagt
      841 gaagaccaaa atatagcgta tgatacttgt gtttacacgc gcgctgaaat agtacgcata
      901 acgaaaacag ccttcgattt tgcccgcaac cgcaaaaaga aagtaacact tttggataaa
      961 gccaatgtac tggcaacatc aagattgtgg cgcgaggtgg taacggaata tgccaaaaca
     1021 caaccagaca ttattttaga gtgtaattat atagatagtg cggccatgga ggtgattact
     1081 aagccctcta agtttgatgt aattctgact gaaaatttat ttggcgatat tatcagcgat
     1141 gaagcctctg tgattgctgg cagtttgggc atattgccat cggcatcaat gggtgaaaaa
     1201 acagccctgt acgaaccgat acacggatcc tatccgcagg caaaaggaaa aaacattgcc
     1261 aacccgatgg ctactgtttt atcggcagcg atgttactgg agtatagttt tggcatgcac
     1321 gaagaaagca gggcaattat aaatgccgtt gaccgtgcga tgaatgaaaa tatcgtaacc
     1381 gaagacatta acaaggggaa atacactacc agcgaagtag gcgataaaat agcggagttt
     1441 gtaatggggt gaaaaaggtt aaaaacacaa ttttaattaa tacttttcat aagttcatga
     1501 aatttgcgcc aatgtctttt tagttgtcca ccaattttgt tgtcaataat agttttctca
     1561 gcgagtttag cataggtaat aaaattaccc caatcatatt tgttagataa aatgaataag
     1621 tctgatttgc ttgggtgtct ttgataaatt cttctccttt cagctcctac aaatgtgaac
     1681 ttcttgtaaa gtcttgtctt gaaaacttga cctttcgttg ctgttctgtt gtgaaatgta
     1741 taaaaccgac ttcttgcgaa agtccgtttc attttgcggt aatagtttga gatactagaa
     1801 ttttttatga gcgtatggta tccgtcaaat tggaaaccta aatattcaaa aagggtgttg
     1861 tcttgaagcc tgttcgtgtt taagtttttt tcaaaacaat aatgtctttc tgttatcttg
     1921 tcatatttga agttgtacac ttttgttttg cttttttgta tttcaagctt atagtttatg
     1981 atagaagtta aaaagtggtc tataatttct tgttcaaatt ctatcgggca aatggcaacc
     2041 atgtcgtctg aatatctttg ataaacccct ccgatatttt taagcatatc gtgtgcttct
     2101 ttgtcaaagt caagcatata gacatttgct aatgtggcac ttatcggaga accttgtgga
     2161 attcctttta cccttaataa atagtcctta ttacccttta tacgcttatt tgttttgatt
     2221 agacctaagt ttcttatttc ttctatattg tctttcgagc aataagctac agctcttttg
     2281 tttcgcaaat agtttttctt ctttattttt atttccttaa acgatttcgg cttccgttct
     2341 acaattattt gatttttgaa attttcaaaa aggtcttctt cttcaatata cgaaaactta
     2401 gtaatgttac taaaaacgtt gtaatggtct tctggtaagt ccgcacctga ttttataatt
     2461 cttttccaat attttttcag taacttatgg tcaaggttgt cgaaaaaact ttttatatcg
     2521 aatgtaattg caactaggtc tttctctttg ttggttttta taaaccgaaa aatatcgtgt
     2581 gcaaaatcaa cattgcattt gttcctgctc ttttcttttt ctgggcatag ttttatttta
     2641 cgataagctg taatactact acttataccc aaatccctta tttcactttc gtaggcagtg
     2701 gatagtattt ctgaataata gctaaacaca tttgagtcta ggtgatttga gaaatatatt
     2761 tcacgttctt ttgttgaagg ttttctcagt tttgaacgtg ttccgtcatg gcatatctct
     2821 ttgcgaaatt tacgtacttt caactgtcta tgaataaatg gataaaatgc gtgttttgaa
     2881 attgcatctt tatctttcaa gtaaggctct atccacgctc tgtcctttgg ctctaattgt
     2941 aatccaacat ggggatagcg ttttagtcta aaccaatctt tttgtttttt gcctttgttg
     3001 atagtttttg ccataagaga aatgaggggt aagtggttag acggggcaag cctttacatc
     3061 aattttcatt aacggaatag tacatgcatt acacaacact ctattgaatg tcgccccgat
     3121 ttccctttcg ggaagaattc tattcgtaac tttgtccata ataataaata ctatgaagtc
     3181 aataagaaca gtacagataa ttctcaacat gcttgaactt tcttgcgatt gtccttgcat
     3241 aattggaatt cctgctccgt aagcaacgta tgtcttgctt agctgtggat gataaaacgt
     3301 actattcagc aaaccactta cctttctcat tttatatttt taaataatat attttagttt
     3361 taacagacat cttttttacc tctccatcaa atatacgcaa ctttttagtt cgtctataca
     3421 atttgtctat atctttttta tgtttatatc atactaattt acagagtaga aaatgaaaat
     3481 agtaaaatcc gtatttttac cccatgaaaa tcgccctttt aggccctgct tacccgtatc
     3541 gcgggggtat tgcaaatttc agcgaaaggc tcgcgcatga gttcatgcag caaggcgatg
     3601 aggtgcattt gtacaccttc aaaatgatgt accccaattt tttatttccc gggaaaacac
     3661 aatatgcaga aacaccgatt ccttttccgc ttcatgtaag ccgaaggata aatgctatta
     3721 atccactgaa ttggtggcga gtggcaaaaa cgatacgtaa agaaaattac gatatacttt
     3781 taacggctta ttctattccg tttttagggc cgagcttggg tacaatttcc agaaaagcaa
     3841 aggcagaaaa cacgaaaacc atatccatta tccacaattt aaaaccccac gaaaaacgag
     3901 ccggagatga tgcgttgtcc gcttattttt taaacagttt tgatgcccat ttggcattat
     3961 ccaaaaaagt agaagaagat attttagaaa gagataaaaa acatcccgtt ttgttttatc
     4021 cgcatccact ctatgataca tttggaaaat tactggataa aaaagaagca atgcacttgc
     4081 taaatttatc aacagaatat aaatatattt tattctttgg attaattcgc gattacaagg
     4141 gattggatat tttattggaa gccatgggcg atcagaggct aaaagataaa aaaataaaac
     4201 ttatagtagc gggcgaattt tacggcggag aagagaaata ccggcaaatc atacaggata
     4261 aaaaaataga agaccgtgtt ttgttgtata accattttat tcccgataat gaagtcaacc
     4321 gctatttttc ggcagcagat ttggtggttc agccgtataa acacgccaca caaagcggca
     4381 ttacacaaat tgcctatcat ttcagcaaac cgatgatagt aaccaatgtg ggaggactgc
     4441 ccgaaacggt gccacacaat aaagtcggtt tggtttgtga gcccaatgca gaaagcctgg
     4501 cgaatgccat tttaacattt tacacagaaa ataaagaaaa gacttttatc gccaatatgg
     4561 atgccgaaaa atctaaatac tcatggaaag gcttagtgga gaagataaaa agagtgaaaa
     4621 acatctaaaa taggagtaaa gtaaaacaaa aatactctta ttttactttc ggggcactaa
     4681 agtaaaataa caaacaagta tcttatcttt atacttatga ttatccgctc taaagcgccg
     4741 ttgcggctcg gcttagccgg aggcggcacc gatgtttcgc cttattccga tatttttggc
     4801 ggtgccatac tgaatgctac catttcgctc tatgcctatg ctaccataaa acctacaagc
     4861 gacggaaaaa tccgtattca tgctattgac agaaatgaaa aaatagaact gctaagcaca
     4921 acagaacttc ccataaacgg acagctcgat ttaataaaag gcgtgtataa ccgcattgta
     4981 aaagactatg cccaccagcc tttatcgttt gatatttcta cttatgtaga tgcacctccg
     5041 ggctccggat tgggttcttc atcaacctta gtggttacta tagtaggtgc atttgtagag
     5101 tggctcaagt tgcctttggg cgaatacgac atcgctcatt tagcctattc catagagcgc
     5161 gaagacctga atatgcaagg cggcaaacaa gaccaatacg ccgctacttt tggtggtgtc
     5221 aattatatgg aattttatgc cggagataaa gtcattgtta atccactgcg cataaaatca
     5281 aaatttttgc acgagctcga acataatttg gttctgtttt acacacagac ctcccggctt
     5341 tcatccgaca ttatcaaaga gcagcaagaa aatgtacatg ccaaaaatga aaacgccatt
     5401 gatgccatgc atcacctcaa gcaacaatct atacaaatga aagaagccat attaaaaggt
     5461 gagatggata atatcggaaa aattttgcac gatggctggc tgcacaaaaa agaaatggct
     5521 aaaggaattt ccaatgcaga aatagatgcg atttactcag cggcactttc ggcaggcgca
     5581 acgggcggaa aaatatcggg tgccggcggc ggtgggttca tgatttttta tgcaccttat
     5641 attcacaaat acaaattgac agacatgctg aatacgctcg gcggaaaagt aatggcttac
     5701 aacttggtta cggaaggctt aaccacctgg acggtttgat gctatttcaa acagtcggta
     5761 gctaaataag ccaatacgtt atttttttcc gtttcggtaa gtttggcttt tggtgccatt
     5821 ttgttcaccc agatattcca aaaatcaata ttgccttttt gtggcgaaaa taactcgtgg
     5881 catttcccgc attttgtggc gtaagttgtt tttccggcaa gataggtttc gcttggtgcc
     5941 gactctaaag gtatgttttc tttaacttcc gttttggtgg gcgtgttttt tttagaacat
     6001 gcaaaagcca gtaagatggt caacaagaag agtgaagttt ttttcataat agattaaggt
     6061 tgataaccgt cagtcaaagt taataaaaat gcttttatat cattgatttc ttccgtagta
     6121 agttttaaat tacccaattc gtctttgttt atatcttcaa caacctcggg agaattaaat
     6181 ttaccgacaa tatcgcgttc actgtaaaaa cgaatggcat cttccagtgt ctgtataaca
     6241 ccgttgtgaa aataaggtgc tgttttttca atgttgcgca aagtaggcac tttgaatttc
     6301 ccattttccc tacttgaatt tttagtgatt cccaacccaa aatcaggaat aagagattga
     6361 tttggattgg gaggcaaccc gatattatca taggtaaaat ctgtaaacaa tacttttccg
     6421 cttatttcat cagcagatga cggatgacat gcactgcagt ttcctttttc aggatcattg
     6481 aataaaatta aaccccgcat ttcttgtcgg gtaagttgca cttttccttt cagataatag
     6541 tcaaacttgc tggtaaatgg acttacttga ttggattttt caaatgcaac aacagccttt
     6601 tgaaacgatt gaataaattg taatgagtct gaaaatacag cttctccaaa tatggaaaca
     6661 aaatctgcac gatacgctga tctattaatc cggtcgatta ccgcagcaat attgttcata
     6721 ttcatttcga tatgactgaa aacgggttct aacaactgct tttcaagaga cgaagccctg
     6781 ccatcatgaa aaaaaccacc aacccaagta ctgtctatat tatcaaaata catttgttgt
     6841 ggagaaaaag ccaaataact gatggagggt gaatttctgt tgcccaacaa acccaaaaca
     6901 cctttggaaa atgctgaatg ttcaggatct gaaaatcctg tcaaaggcgc atgacaactg
     6961 gcacatgaaa taccaaccgg attggataag tttttatcaa aaaatatttt cttacctact
     7021 aattctaacc tggtaagtgt ggtcgtgtca ttgataaaat cttgctcgct actgtaaaat
     7081 ggttgaacaa cttcagttgg cgtacatgca tttaatagca caagggcact cagcatacgc
     7141 acaaatcgac taaaatgcat acgaaaagat taagcgggtt tgcaatcgtt caaattcagt
     7201 cagattgagt tttcgatcat aagcattgcc tttaaaccct gctatttgag gtaatatggt
     7261 agccgttatc caaaaattgt ccatacgcca ggatgcattg ggaccaaaat acattgccga
     7321 atgttctaat ttttcagttg gcgtaaatac gtttttctga acagcttcca aaccaactgt
     7381 aaatcctttt ttcaaaaaat aacaaacacc aaaattaaag tcgagtttaa attcatgatt
     7441 tcgtttcatt ttcaggtttc cgtttgcata tgcaggttct ttttcaattt catattcaaa
     7501 aacggtattg tgtacaaaca gccatttttt tacttgtttg tctaatatga gcttaccctc
     7561 aagttcaatt tcattcagac ccagcgttaa ttcaccatat attgcagagc ctaacttttg
     7621 ggcaaccgga ttactgaact ggtgtttcca ttcattagaa aagctaaatt ctgtttcttt
     7681 ttccagctcc ggttcagagc cattgggaat ggcagtagcg ctatgcccga tattcaggta
     7741 aaatgcggtt tgcgtgtttt tgcctatacc tatttcaaat tctattcggg gttcaaaggc
     7801 cctgaagaag gtttcttttt taaaacggaa cgtattccag acttcaattt ctttttgttt
     7861 cttttccagc acattgcttt gatagatata ggtaaagaac ctgtcattag cataagaagc
     7921 tatactaagg aagaataaag aaaggaaggt aaacttaagc ttcattttgt ttttatttag
     7981 accaattata aatagatgcg caaaaataaa aacttcttac tttgtaaagg ctataaataa
     8041 taaggaaaat gctacctaag tttaggtaaa aatcagccga ttttcacgga agtcatggtt
     8101 aatgaacccg caactgcttt gtcattaaag atgtaagctg tttcattagt ttcctgcaat
     8161 cccaaaatat ataataaagg taggtaatgc tccggagtgg gtatggccaa gttaaaggcc
     8221 tgtccaagag tatgataatg aattaaaggc tgatggtttt tagctgaaat aaaacggttt
     8281 acatcagttt gcgcttctct tgcccaatca taagcataat tgtcctcttc cattttattc
     8341 caggcaatcc gtcctaaatt gtgaacgata ttaccacttc caagaataag cacccctttg
     8401 ttgcgcaaaa atgccaattg ttttgctaag tcgtaatgat attgaggtga ttggatataa
     8461 tccaaactaa gttgcaaaac cggaacatca gcttttgggt ataggtgttt cagcaccgac
     8521 cacgcaccat gatctaatcc ccatttttca tccaagccaa tcttagtacg ggaaacgtgc
     8581 tgctgaatag tatttgctaa agcaggttga ccatgtgcag gatactgcac ttgataaagt
     8641 tcttcgggaa agcctccaaa atcatgaata gtaggcggaa actccatagc ggttacggca
     8701 gtacctttgg tttcccaatg tgcagaaata caaagtacgg cttgaggttt ctcaaaagta
     8761 gttgcaattt gttcaaagcc caacgtaaac tcattaacag taatggcatt catggggctg
     8821 ccgtgtccaa ggaagagcaa aggcattttt tctgaattat tcaattgcgt tgcaaatccg
     8881 actaattctt tggctttcat agtagagttg ataagtgaaa aggctgataa taattgcaga
     8941 aaagtcttcc gcatcataaa ttaattctaa ttgtattttc ttttatatta taataaaaaa
     9001 taaatttatg ccgatgttgc ttgaaaatag taaaatcgaa agtagtgaag cttggactat
     9061 attgaaaaca catatggtat taatcccaat tgtttttgga agaaacgcca aactttccgc
     9121 ctcctgtaaa gaataaggct aagccaccaa agaaatacaa tccttgtagt tctaaagccc
     9181 agctgccgtg ttcagtaagt ttgaatacat ctccggaatg tgccatcgcc acagcaacta
     9241 ccatattaaa tgcgaaaaca aaggcagcta tacgggtacg aaagccaata atcatcaaga
     9301 caggagcaat gacctcacca ataataaccc cataagcaat aaaagcagga agtcctttat
     9361 ctgtaagcat atcaccaatc atagatactc cactttgtag ttttccggca ccgtgaaaca
     9421 acattaatgc acccaaacta acacgcagta tcaataaacc aatgtcataa ttttttttca
     9481 taaacgaaga ttatttgcaa ttaaagatgc gaaaaattta gcatatacgg ttaataaaaa
     9541 ctttatgcat actcaaagcc agctacatta taaaaactgg ggatgacaat atttacctcc
     9601 atgtcttctt cgttaaaaaa tggagatggt gcttatgggc ctagttctat aaaataaaaa
     9661 gatctttttc atctgtaaac cacctaatcg aaacgtatgt ttatatctac tataagattt
     9721 tacatctgtc agaaatgact aaaaaacacg agaataattt tgtgtttttt caaaaaaaat
     9781 tatcattcat tatcaacgca ttatgccata atacaggcgt tctggcacaa atagttaatt
     9841 tgtttaatgt gttgtaagtt actgataatc aggaagaatt tttttttatt ttttttgaga
     9901 cccagccata ggttggctta gtctcgttat aattttgtcg tatcaaatct aacaacatga
     9961 aaagaataaa tcaaatacgg ctcaagaata aggatgatga catttccctg tttacaagta
    10021 cgaacattag ataacaaatt ttttaaacaa ataaactata ataaaatggc aatcacaaac
    10081 ataaacggcg tgccctatct ggaagattat gatccggata agccacttgg gaaacaatta
    10141 tatgagtggg gagaacgctt tggcacatca accggaaata acacgcaaac aagcggaact
    10201 acaagaaact tttttgccca atcaccgaat agaaatatgc atcttagtgt agatgaaatt
    10261 gtcacggcag gcttatacag gaaaacatat gaccaggaca atacacgtgt atatatggtg
    10321 aactctaaat tgagatttaa taatgaacga gcctttacat tacctgaagt attaattaaa
    10381 gcaaaagctt tggaagatag gtttataaaa gaatttgacg gatttgaatt aatcttattt
    10441 gctaattctg acggagcggt aatattacaa cattttgagt atgcaacacc gaaacccaac
    10501 cagcttatcc tgtttttgga aataaatgat aagacaaatt atttcggttc tgtgatgagg
    10561 gtaaagcttg gagaaagaat aattacatca aaactaccct ttacacaaat ttatgaattt
    10621 tcagaagcac accaagtgaa aatatcaaaa gatgagataa ttgatctgat taatgatggc
    10681 aacctgaaga ataagtttcg gtatatattg agggtgctta acaaaatctc aaatccggta
    10741 agttatatca gtggaatagc attcagcaaa atgggagatg tatttaaaaa taccattcct
    10801 gattttttag aagaattgaa aatagctgaa aatcgttgga atcctgatgc ccaggggtta
    10861 ttatccttta tttggaagca gaggattaga gcttatggta caacaaagta taagggcaaa
    10921 agtaaaacct gacaaccact ttaagcttgt acaaaaaacc gtagatgata tgtttgataa
    10981 aatagaaagc agaatagatg cattttataa gtttatcacg caatattttc caaattcgtt
    11041 aaacagaaaa tttcatacaa tattaaaaac attaaatacc aagctaaaag atgcgcgcaa
    11101 agtatttaac gattcagatt ggctttccaa actattagag ctcggcaccc aaatgttcaa
    11161 tgcctatctg tgtggcatta taaacggttt tattgatgcc attaatgggg ttttccaact
    11221 tataggatta gtatgttatg ctatagcagc actcaataaa ggcgcatccg atatattgtt
    11281 tgctgcgtcg atgttggtag aagtatttga aaatattttt gaggccatca ttgaatttga
    11341 tttaataggt tttttgtgga agtgcgtaaa actgcctttt aaatttgtgg taggtatata
    11401 taaattttta aaacaaatta ccgaaaccga tttaaaaaag ttactaaaga agttagatac
    11461 ctccttagtt aaaattgctt atgtttgtgg taatatagta ggatttatta tacaattgct
    11521 gttagaggca ttgtgtacgg gcggagtggc ggcagtagaa gacttgatac agacactgtt
    11581 caaatttttg cgtaaccctg cagaaagcat agccaacggc ataaaagcta cgataaatat
    11641 aagcaaagag ttcttttaca atttaattta taaactcagc aagttctttg agtccttaaa
    11701 aagcaaagcc gcctataaag ccatagacga atgggtagag caactacttg cagctattaa
    11761 aaaagagttg ggtattagtg atgagg
//
LOCUS       DAOQII010000054        11538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2819_length_11538_cov_4.880606, whole genome shotgun sequence.
ACCESSION   DAOQII010000054 DAOQII010000000
VERSION     DAOQII010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11538
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2819_length_11538_cov_4.880606"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLF48_04080"
     CDS             complement(<1..362)
                     /locus_tag="PLF48_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF48537.1"
                     /translation="MNIKRRRAFHDEDAMASMNDIMFFLMLFFLIISTMANTNVIKLM
                     LPKANSTEKLAKQPLTLSVTDGKEYYINTTKIPLEGLENALQAALENNPDPTVVLRVS
                     QNLTVQDLVDVMAIGAKL"
     gene            complement(675..1382)
                     /locus_tag="PLF48_04085"
     CDS             complement(675..1382)
                     /locus_tag="PLF48_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNF48538.1"
                     /translation="MGIFLQAATTLIDTTGQAAAAAAAAAPKEELSVLTLLFKGGVMM
                     IPLLVCLALAVFFIIERLLFISKRTNINEGLVRSVLDKLYEGNLQGADALCTADKSAL
                     GNVLQAGIGQLGKPIDHIEKALDIQANIELNDMEKRTSYISLISGIAPRLGFIGTIIG
                     VIVIFYNIASSGGNISIDTIASGLYQKMISSGMGLIVGVIAFMGYHFILTRIDSYSMK
                     LQRQIFEFVKGIQKPVK"
     gene            1545..2786
                     /locus_tag="PLF48_04090"
     CDS             1545..2786
                     /locus_tag="PLF48_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF48539.1"
                     /translation="MPEFNLNKSIPGAAYAFSFVHITDVHVGEDYNQNGNDYGGNGFD
                     NDSVPYGDNSRPVRALKNAVTWINENAAAKNIKFVIVSGDLTGSAEKSEMIHCRDILN
                     KLQIPYVPIIGNHDIWPYVRYNYEARYAYGDSVWNEVFDSVFTANKFFFENWDDGTRL
                     QRTYDSEDSLGHYFQNFSFEYDDFVFYGLDFNPRYHVHKAEPGIGPEAQLMDWNGGTF
                     RWLKQQLASNTHLGNENTILIAHHPITDNLIMIASGFVFERDEYDKMVSMLAPYKNNL
                     ALWLSGHIHFDTDYYLSNILRVRTMAANKDFAQSRFEVVNVYQTQQVTGLKENKNPEL
                     SCYPNPNNGIFNLELNNIDKDATLIITDLSGKIVLTENILQHQDQTKYHFDFSTLPKG
                     IYLLNITNKNASLETIKFVIQ"
     gene            2789..4003
                     /locus_tag="PLF48_04095"
     CDS             2789..4003
                     /locus_tag="PLF48_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNF48540.1"
                     /translation="MFSFRKTVVCYSTLLLCNFISCNSNHSNQSRTGNEQSLISGTLT
                     RDSILGVKYLNKKYLDAIEKINVNPQKSEDTKGMLHIKGGTFRMGGDRPAGFENMPST
                     ALAQADEFPKHEVRVSDFLMDEHEVTVGEFLEFVKATGYKTVAEQDIDWEELKKQVPA
                     GTPKPKDSLLKAGALVFHLVRRNENPDNLDDWWTYTPGANWKNPDGKQDKLKEILRYP
                     VTQVSWYDAMAYAKWVGKRLPTEAEFEYALRSGKQNNMYPWGNEKLNDKKYYGNFLQG
                     DFPYTNTVKDGYALTAPVKSFPPNAYGLYDIVGNVWEWTNDWYGADYYKVLADRGEPV
                     QNPFGPEKTYEVNDPYAVSKVIRGGSFLCSDGWCSGYRNSRRMRLSPDSGMQHVGFRC
                     VRDIDLSSGYKK"
     gene            complement(4158..5078)
                     /locus_tag="PLF48_04100"
     CDS             complement(4158..5078)
                     /locus_tag="PLF48_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48541.1"
                     /translation="MLLSVSLGLIFIVSGATKLMSMELFEYQFVEIGVAGWKTAPYFA
                     RFLIGLEFFIGILLVLNIAVKKFSAPLAIGILLFFSVYLIYFLIVYGNKGDCGCFGQA
                     LKMSPLAGLIKNIALLIVAFILLWQYDTTFWKKKITRILTPILAIISMCCAFFIYPIN
                     VFAHYQFDNNKTDYKVALDLMYDSTQTYKPTQELRNGKHIVAFFSLTCPHCKMAAKKL
                     KIIHHRHPELPLYIALNGEPKDTAVFFNETEVQQIPHNLFLGPQSWLMVAGIQLPVIM
                     YLENGIVKKKVNGINLDEDDALNWSQQTIR"
     gene            5240..5707
                     /locus_tag="PLF48_04105"
     CDS             5240..5707
                     /locus_tag="PLF48_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48542.1"
                     /translation="MEIAQFSNLSDEEKELMMNTPALVTVLLAGVDGEIDMTEEEQAN
                     RLVHIRATTGDPILFDFFKLLESEFNTQLSVMNGKYMNFEKEVRVQEITEELSKLNDI
                     LPKLDPLFARAFLKSMRSFAGSVAESTGGILGFLSVSFDEKQLIGLEMITYEP"
     gene            5707..6207
                     /locus_tag="PLF48_04110"
     CDS             5707..6207
                     /locus_tag="PLF48_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNF48543.1"
                     /translation="MDTPTSIQKKTIPPQLQKLAKWVKLMDSQWRIPGTNITFGVDPV
                     LDLIPGLGTIVDYGISAYLMFALWKNGSSSKLLLKMFTNITIDGIVGAIPVLGNIFDV
                     FYKANRKNLVLAVEHYEEGKHSGSALPYLIPVLIVLALVFIGLAFCSYFALKFIFGFL
                     SSMNLS"
     gene            complement(6222..7430)
                     /locus_tag="PLF48_04115"
     CDS             complement(6222..7430)
                     /locus_tag="PLF48_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48544.1"
                     /translation="MSNWTKTTTASPNALETPGTWNSANNQTWILSLRQPFNAVMDDQ
                     SYTCDLRAFESRYYLDGNDSIIYWIKNNGATDHGPYTAQTIIHNTATTGKGFQNSNLT
                     GTPLFLQKTFAGTPVTYTPANYKVKFFFSTQELQDYCDYINPILNALPGYYTGHNYTP
                     STVKPHLKIYRTSLTDRAWTVTSDAQVQIVSPVLGTYGAYTTFEYDGFTGFSGYALGD
                     VVTPIIGLPVELKQFEATCSDDNRVISWTTASERNTRNFEIQYSYNGSNFYQLAVVQA
                     IGNSVTEQHYSYTDNSASDKVYYRLKVIDNDNSFSYSQVVVADCADTYTEPQLYYDAL
                     HANYIIQSSGKAIVTSYTLYDIAGKIVRHTTIDHTFTTPVDMENLPAAVYILKMNTEN
                     NAYYKKVVKH"
     gene            complement(7614..9923)
                     /locus_tag="PLF48_04120"
     CDS             complement(7614..9923)
                     /locus_tag="PLF48_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF48545.1"
                     /translation="MKKIYTLITFLFIVIIQCNYAATLTEDFEAVAFTKNAYDVAPAG
                     NGTEDNVTFASGSWRIFDALRGNTAGSDRFNGTQSVRARSNTSIVSMNFDKSGGAGIV
                     SVYAAKYGGDANSTFQFELSTDGGTTWPNVSPTFSLTSTTLTQFTWNANISGNVRIRI
                     VKLTTTSTSNRCNYDDFSITDYTGSVPEINLQQPAGSDSACGMTYNFGTQTIGTNTDV
                     TVRIQNTGTFQLTISGTPITGTNASEFSVFTAPTSPVAASSYTDMTIRFSPGTPGIRT
                     AVLTINSDDADESACIINLTGNANYAPCTELIISEYGEPVVGSGKYIELYNGTSGAIN
                     LANYALWRVSNGAVLWSGATVMSPALSGTLAAGSTYVIANNATDVPGANLYNATFCNW
                     NGDDAIGLAKNISGTWYLIDAVGTDMTGYAGTGGWSVAGISGATTDHTLVRKTSVTAP
                     NADWNTSAGSTATNSEWFVRPYQLSNVGCNVNSCSVSSTVGFVTTSTAVTEANTTVTV
                     NATMNSAPTSTVNVKITDALLGTATSGTDYTAFSPVTLVFTPAETYPNTKTFTVSILD
                     DAFSETNELITLDLDAQCGALVSNSRHTITIIDNEIPEGVVINEISQGSANKEYVELV
                     ITGTPGTTMDLRGWIIDDNSGIFSGGYGTQLGIAPGHIKFSDICTWEKVPVGSIILLY
                     NASDKNVSITIPDDPTDGNLDYTYVVPIYASATTCAAMATSNLYFSSDCDKPNNVSYD
                     QYTPPVYTNVDWNAMQLRNGGDAVQVRSS"
     gene            complement(10215..10892)
                     /locus_tag="PLF48_04125"
     CDS             complement(10215..10892)
                     /locus_tag="PLF48_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48546.1"
                     /translation="MKKVIIPIAAMLFFIACKPKNESTENASTPDAVVTANEAKADPN
                     VTDWMSWENGIDIAGVTDPNLKMPNVIFHVAHMVSTPLGNAPSGMILYQPDSTKPPMV
                     MGFISTDSKVGGYFGPKIFAGTPFEKAPVLPATFDIKTTANGATAKVVTGGHTFEAEV
                     TDLGAPYLINRAPAAMSPFYQQGVERKIGKATLKVDGKDVAITIPPVGISGGPGAVFS
                     PNGVYAR"
     gene            11047..>11538
                     /locus_tag="PLF48_04130"
     CDS             11047..>11538
                     /locus_tag="PLF48_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48547.1"
                     /translation="MNKFFKLTYILLFLYTFNTLKSANELYINGPGSTTSTATLYDST
                     CLIFVNGEIKNNDGLFINRGGTIELTGNWTNTVSSNKYQSTGTEKFYGTSEQVISGTL
                     NGTTYTTNAYDNQFCHLKVYRKSTITTTPAKYISLGANVNVAGSISFETSTPNTTGQE
                     QVQM"
BASE COUNT         3578 a         2118 c         2230 g         3612 t
ORIGIN      
        1 ttcagtttgg ctccaattgc cattacatct accaaatctt gtacagtcag attttgagaa
       61 acgcggagta ccaccgtagg atcaggattg ttttccaagg cagcttgcaa cgcattttcc
      121 aaaccttcaa gcggaatttt tgtagtgtta atataatatt ctttaccgtc tgtaacagat
      181 aaagtaagcg gttgtttcgc taatttctca gtactgttgg cttttggcag catcagtttg
      241 atgacatttg tattagccat cgtagagata atgaggaaaa acaacatcag gaagaacatg
      301 atgtcattca tggacgccat cgcgtcttca tcatgaaaag cccttcttcg ttttatattc
      361 atcttagatt agttgtttgt tattagttat tggtcattgg taattagtga ttagagattg
      421 gttattggtg attagagatt ggtgattggt tattggtcat tggtgattag tgattggtta
      481 ttggtgatta gtgattagtt attggttatt ggttattggt gattagtcta tttcttgatt
      541 cttgactctt gattcttgac tcttgattct tgactcttga ttcttgattc ttgactcttg
      601 attctcatat ctcatatctc atatctcact acttactact tactacttac tacttactac
      661 ttactactcc ctactcactt caccggcttc tgtatgcctt ttacaaattc aaaaatctga
      721 cgttgcaatt tcatcgaata gctgtctatg cgggttaata taaagtgata acccataaat
      781 gcaattactc ctacaattaa gcccatcccg gatgaaatca ttttttggta taaaccactt
      841 gcaatcgtat caattgaaat attaccaccc gaagatgcga tattataaaa gataacaatt
      901 actccgatta tcgtaccgat aaatcccaaa cgtggcgcaa tacccgatat caaactgata
      961 taagaagttc gtttttccat atcattcaat tctatgtttg cctgtatatc aagcgctttt
     1021 tcgatatggt cgatgggttt tcctaattgt ccgatgccgg cttgcagtac attacccagc
     1081 gcggatttgt ctgcagtaca cagagcatcc gcaccttgca gattgccttc atacaattta
     1141 tctaatacac ttctaaccag gccttcgttg atatttgttc gcttgctgat aaataatagt
     1201 cgctcaatga taaaaaatac ggctaaagcc aggcacacta atagtggtat catcattaca
     1261 ccacctttga aaagtagtgt aagtacagat agttcttcct tcggagcggc agccgcagcc
     1321 gcagcggcag cttgacctgt tgtgtcaatt aaagtagtgg ctgcttgcaa aaatattccc
     1381 atgcgctgat attaatatgg acaaaattaa gattttactc taaacgaaca agaatttgtt
     1441 ttattgtttt ctgtaaattc gctattttta gtttaatgaa atttaagatt cttatttttt
     1501 acttcctctt tcttactttc tctggttttg ctcaatccat ttccatgcca gaatttaatt
     1561 tgaataaatc tattccaggc gcagcatatg ccttttcatt tgttcatatt actgatgtcc
     1621 atgtgggaga ggattataac caaaatggaa atgattatgg aggcaatggc tttgataatg
     1681 acagtgtgcc ttacggggat aatagccggc ccgtcagagc actgaaaaat gcagtaacct
     1741 ggataaatga aaatgcagct gcaaaaaata taaaatttgt catcgtttcc ggcgatttga
     1801 ccggtagtgc agagaaatca gaaatgatac attgccgcga tattttaaat aaacttcaaa
     1861 ttccttatgt acccattatt ggcaatcatg atatatggcc ttatgttcgc tataattacg
     1921 aagcccgtta tgcttatggc gattcggtat ggaatgaagt ttttgacagc gtatttacgg
     1981 caaataagtt tttttttgaa aactgggatg atggtaccag gctacagcgt acgtacgact
     2041 cggaagattc tttaggacat tattttcaga atttttcatt tgaatacgat gattttgtat
     2101 tttatggttt agattttaac ccgagatatc acgtacataa agcagaaccg ggcattggtc
     2161 cggaagctca acttatggat tggaatggtg gcacattcag gtggctgaaa caacagctgg
     2221 caagcaatac tcacttggga aacgagaata ccatactgat agcacaccac cccattacag
     2281 acaatctgat tatgattgcc tccggatttg ttttcgagcg agatgaatac gataaaatgg
     2341 tgagcatgct cgcaccctat aaaaataatc tcgcactctg gctttccggg catattcatt
     2401 ttgatacgga ttattacctg agtaatattt tgagagtacg caccatggct gcaaataaag
     2461 attttgcaca aagtcgtttt gaagttgtga atgtatatca aacgcaacaa gtaaccggac
     2521 taaaagaaaa taaaaatccc gaattatcgt gttatccaaa cccgaataat ggtattttta
     2581 atcttgaatt aaataatatc gataaagatg caacattaat cattacggat ttatctggta
     2641 aaatagtttt gactgaaaat atactacaac accaagacca aactaaatac catttcgatt
     2701 ttagtacact ccccaaagga atatatttat taaatattac aaataagaat gcttctctcg
     2761 aaactataaa atttgtaata caataacgat gttttctttt agaaaaacag ttgtttgtta
     2821 tagcacttta ttgctctgta attttataag ttgtaatagt aaccactcca accaaagcag
     2881 aacaggaaat gaacaaagtc ttatatcggg tactttgaca cgggattcaa tacttggagt
     2941 aaaatattta aataaaaaat atcttgatgc tatagaaaaa ataaatgtaa atccccaaaa
     3001 atcagaagat acaaagggaa tgctgcatat taaaggaggc acgtttagaa tgggtggaga
     3061 cagaccagcc ggatttgaaa acatgccctc aactgctctg gctcaagcgg atgaatttcc
     3121 caaacatgag gttagggttt cagatttcct gatggatgaa catgaagtta ccgttggcga
     3181 atttcttgaa tttgtaaaag caacagggta taagactgta gctgaacagg atattgactg
     3241 ggaagaatta aaaaaacaag ttccagccgg gacacctaaa cctaaggata gcctacttaa
     3301 agccggagca ttggtctttc acttggtgag aagaaatgaa aatcctgata atttagatga
     3361 ctggtggaca tatacacccg gtgcaaactg gaaaaatccc gatggaaaac aagataaact
     3421 aaaagaaata ttacggtatc ctgttacaca agtatcatgg tatgatgcta tggcatatgc
     3481 taaatgggtt ggtaagcgat tgcccacaga agccgaattt gaatatgcac ttcgcagtgg
     3541 caaacaaaat aatatgtacc cttggggaaa cgaaaaactg aatgataaaa aatattacgg
     3601 caactttctg caaggtgatt tcccgtatac aaatacggtt aaagatgggt atgcactaac
     3661 agcacctgtc aaatcatttc cgcccaatgc ttatggcttg tacgacatag ttggcaatgt
     3721 ttgggagtgg acgaatgatt ggtatggagc agattattat aaggtacttg ctgatagggg
     3781 agagcctgtt caaaatccgt ttggacctga aaaaacatat gaggttaatg acccttatgc
     3841 cgtaagtaaa gtcatacgcg gaggttcttt tttgtgcagt gatggctggt gttcgggtta
     3901 tagaaattcc aggaggatgc gtctttcgcc tgacagtggc atgcagcacg tgggttttcg
     3961 atgcgttaga gatattgacc tgagttcggg ttataaaaaa taaggggata aaatagttga
     4021 tttctcattt ttttctccac tttaacaagg tggaggaatt aaaaggaggt ggttctatat
     4081 aaaaaatcaa ctatttttta attgaaacgc ttatctcgaa ctcaggttat taaatcgaac
     4141 taaagaaaga cagcacgtta tcttattgtc tgttgagacc agtttagagc atcatcttca
     4201 tccagattaa tgccgttcac tttctttttc acgatgccat tttcaagata cataatcacc
     4261 ggcagttgta tacctgctac cattaaccac gattgtggac ctaaaaataa attgtgcgga
     4321 atttgctgga cttcggtttc attaaaaaat acggcagtgt ctttcggttc tccattaagg
     4381 gcaatatata aaggaagctc tggatgccta tgatgtataa tcttcagctt ttttgctgcc
     4441 attttacaat gcggacaagt taaactaaaa aatgctacta tatgctttcc atttctcaat
     4501 tcttgtgtgg gtttgtatgt ttgtgtagaa tcatacatta aatctaaggc aactttataa
     4561 tctgttttat tgttgtcaaa ctggtaatgg gcaaatacat taatgggata aataaagaaa
     4621 gcacaacaca tggaaataat tgctaaaatc ggcgttaata ttcgggttat cttcttcttc
     4681 caaaaagtgg tatcatattg ccaaagtaaa ataaaggcta cgatgagtaa ggcaatattt
     4741 ttgattaaac cagctaaagg cgacattttt aatgcttgtc caaagcaacc acaatcaccc
     4801 ttattgccat aaacaataag gaaataaatc agataaacgg agaagaaaag caggatacca
     4861 atagctaacg gcgcactgaa tttttttaca gcgatattca gcactaataa aattccgata
     4921 aaaaattcta atccaatcag gaagcgggca aaatagggcg ccgtcttcca accggcaaca
     4981 ccgatttcta caaattgata ttcaaataac tccatactca ttaatttcgt agctcccgac
     5041 acaataaaaa ttaagccaag tgatacactt aacagcataa gtagtagtgt tttgatattt
     5101 aatgccattg tcatgccaaa ggtaaataaa aatcaaaagt atctttaatt tgttcaagga
     5161 ttaatttttt ttgcattgtt cacataaaaa ttacatgcat tttagtgtga ttctcttaat
     5221 tttgcaaaaa aaagagtata tggaaatcgc acaattcagc aatctaagtg atgaagaaaa
     5281 agagttgatg atgaacacac cggcattagt aactgtgctg cttgcaggtg ttgatggaga
     5341 aattgatatg acagaagaag agcaagctaa ccgtttggtt catattcgtg caacgaccgg
     5401 agaccccata ttgtttgatt ttttcaaact gctcgaaagt gaattcaaca cacaacttag
     5461 tgtaatgaat ggtaaatata tgaacttcga aaaagaagta agagtgcaag aaatcacaga
     5521 agaattaagt aagttgaatg atatattgcc taaacttgat ccgctgtttg cccgggcatt
     5581 tttaaaatct atgcgctcat ttgccggtag cgttgcagaa tctacgggcg gaatattagg
     5641 ttttctttcc gtatctttcg atgagaaaca actcattgga ttggaaatga taacttacga
     5701 gccataatgg atacaccaac aagcattcaa aaaaagacca tcccgccaca gttgcaaaaa
     5761 ttggcaaaat gggtaaaact catggacagc cagtggcgca tcccgggtac taatatcact
     5821 tttggtgttg atcctgtttt agatttgata cccggtttgg gtactatagt agattatggt
     5881 atttcagcct atctcatgtt tgccttgtgg aaaaacggta gcagcagtaa acttttacta
     5941 aagatgttta ccaatattac tatcgatggt atagttgggg ctattcctgt tttgggaaat
     6001 attttcgatg tattttataa agccaaccgc aaaaatttag tattagctgt tgaacattac
     6061 gaagaaggca aacatagcgg aagtgcgctg ccatatctaa ttccggtttt aatcgtattg
     6121 gctttggtat tcatcggttt ggcattctgc tcctattttg ccctgaaatt tatttttggc
     6181 tttctcagca gtatgaactt aagctgagta ttaaaaacga attaatgctt aactactttt
     6241 ttgtaataag cattattttc agtattcatt ttcagaatat atacggctgc aggcagattt
     6301 tccatatcta caggtgttgt aaatgtatgg tcgatagtcg tatgacgaac aattttcccg
     6361 gcaatatcat acaaggtata agatgttacg atagcttttc ctgaagattg aataatataa
     6421 ttagcatgaa gtgcatcgta ataaagctga ggttctgtat aggtgtcggc acaatctgct
     6481 acaacaactt gtgaatagct aaatgaatta tcattgtcaa ttacttttaa acggtaataa
     6541 actttgtcag aagccgaatt atcggtgtat gaatagtgtt gttctgtaac agaatttcca
     6601 attgcctgta ctacggcaag ttgataaaaa ttacttccat tataggagta ttgtatttca
     6661 aagtttcgcg tgttgcgttc gctggccgta gtccacgata taactctatt atcatcggaa
     6721 caggtagctt caaattgttt taattcaacc gggagaccaa taatcggggt aacaacatcg
     6781 cctaaggcat aacctgaaaa acccgtaaat ccatcatatt caaaagtagt ataagctcca
     6841 tacgtgccaa gtacaggtga aacaatttgc acctgtgcgt ctgaggttac ggtccatgct
     6901 ctatcagtta gtgaggtgcg gtagattttc agatgcggtt tcaccgttga aggcgtataa
     6961 ttatgcccgg tatagtagcc gggtagggca ttcagtatcg gattgatata atcacaataa
     7021 tcctgtaatt cttgagtaga gaaaaagaat tttactttat agtttgccgg cgtataagta
     7081 acaggtgtgc ctgcaaacgt tttctgcaaa aataagggtg taccggtcaa gtttgaattt
     7141 tgaaaacctt tccctgttgt agccgtattg tgtataatag tttgagccgt gtatggacca
     7201 tgatcggttg ctccgttatt ttttatccag taaatgatgc tgtcattgcc atctaaataa
     7261 tagcgactct caaatgctcg taaatcacaa gtgtaagatt ggtcatccat aacggcatta
     7321 aatggctgtc tgagtgataa tatccaggtt tggttattag cagaattcca cgttcccgga
     7381 gtttctaatg catttggaga agccgttgta gtttttgtcc agttactcaa ttttctaaaa
     7441 tcattatcaa tagcattaag gttggcataa gtcgtatttg atgtgccaga aaaatataaa
     7501 gcatctgtac cataaaccga ataatcaggg tgatttgttt gatttaatgc acatgtgccg
     7561 caatccgaac cacttccctt gctaccataa gagatgccct ggaagaaacc accctatgaa
     7621 ctgcgtacct gcacggcgtc accaccgttt ctgagctgca tcgcattcca gtccacgtta
     7681 gtataaactg gaggagtata ctgatcataa gaaacgttat taggtttgtc gcaatctgat
     7741 gaaaaatata gatttgacgt tgccattgcc gcacaagtag tagcagaagc ataaatcggt
     7801 acaacatacg tgtaatccaa attgccgtct gttggatcat ccggaatcgt tatgctaaca
     7861 tttttatcac ttgcattata caataaaatg atggagccaa ccggaacttt ttcccatgtg
     7921 caaatatctg agaactttat atgaccgggc gcaataccaa gttgcgttcc atagcctccg
     7981 ctaaaaatcc cggagttgtc gtctataatc catccgcgca agtccatagt tgtgccgggt
     8041 gttcccgtta ttactaattc tacatattct ttattagcac tgccctggct gatttcattg
     8101 atgaccacac cttccggaat ttcgttgtct ataatagtaa tcgtgtgtcg actgttgctg
     8161 actaatgcac cacattgtgc atctaaatcc aaggtaatta attcgttcgt ttctgagaaa
     8221 gcatcatcta gtatagatac cgtgaatgtt ttggtattcg ggtatgtttc tgccggggta
     8281 aacactaagg ttacaggaga aaatgcggta taatctgttc ccgaagtagc agtacccaac
     8341 aatgcatctg taattttcac gttaactgtt gaagttgggg cactattcat tgttgcgttg
     8401 acagtaactg ttgtattcgc ttctgtaacc gcggtacttg tcgttacaaa tcctacagta
     8461 ctggatacag aacaagaatt tacattacac ccaacattac tcagttggta agggcgtaca
     8521 aaccactctg aatttgttgc agtacttccg gctgaagtat tccaatcagc attgggagca
     8581 gttactgaag ttttacgaac taacgtatgg tctgttgtag caccactaat tccggcaaca
     8641 ctccatcctc cggttccggc atatccggtc atatctgtac ctacggcatc tatcagatac
     8701 caagtgccgg aaatattttt tgccaaacca attgcatcat caccgttcca gttacaaaaa
     8761 gtggcattgt aaagatttgc acccggaaca tccgtagcat tattggctat tacataagta
     8821 cttcccgcag ctaaagttcc gcttaatgca ggcgacatca cagtagcacc tgaccataac
     8881 accgcaccat tactcactct ccatagagca taattggcta aattaattgc tccggaagtg
     8941 ccattataca gttctatata ctttccgctc ccgacaacgg gttcgccata ttcagatata
     9001 ataagttcag tacacggtgc ataattcgca ttgccggtta agtttataat gcatgcactt
     9061 tcatcagcat catcagaatt aatggttaat actgccgttc ttatgccggg cgtacccggt
     9121 gaaaatcgga tggtcatatc ggtatagctg cttgcagcta caggagaagt aggggcggta
     9181 aaaacagaga actcagaggc attagtgcct gtaatgggag ttcctgaaat agttaattga
     9241 aatgttccgg tattttggat tctaacagta acatccgtat tcgtgccgat agtttgtgta
     9301 ccaaaattat aagtcatgcc acatgcggag tcacttccgg caggttgttg caggtttatc
     9361 tcaggtacag agcccgtata atctgtaatg gagaaatcat catagttgca tcgattagaa
     9421 gttgatgtag tagttaactt aacaatccgt atgcgtacat ttcctgaaat attggcattc
     9481 catgtaaatt gagtgagtgt cgtagatgtt aaagaaaatg ttggtgatac atttggccaa
     9541 gtagttccac catctgtaga aagttcaaat tgaaaagttg aatttgcatc accaccatat
     9601 ttagcagcat atacacttac aatacctgca ccgccggatt tgtcaaaatt catggaaaca
     9661 atagacgtgt tacttctcgc acgtacagat tgcgttccgt taaatctgtc tgacccagca
     9721 gtattaccac gtaaagcatc aaaaatgcgc catgaacctg atgcaaaagt tacattatct
     9781 tctgtcccat tacccgctgg agcaacatca taagcatttt ttgtaaatgc aactgcttcg
     9841 aaatcctcgg taagtgttgc tgcataatta cactgtatta tgacaatgaa taagaatgta
     9901 ataagcgtgt agattttttt cataagcaaa taatttacaa atttagctca atgattttca
     9961 acgcaaatca attttagatt ataaatacgt gttttgaatg cgtatttaaa agtaaaacta
    10021 attctccata aaaatgttcg agatttgtta aatactcagg taactgtgtg cataagcgat
    10081 attatttgtg tataatcaaa tttaagccta ttctgtatga tgaattttgt gcatatgttt
    10141 aggtgttgtt cttaattgtt aagaaaaaag gcggctaaaa aaatgccgcc tgaaaaattt
    10201 tattttatta taaattatct ggcatagact ccattaggag aaaatacagc accgggtccg
    10261 cctgaaattc caacaggagg tatcgtaatt gctacatctt ttccatccac cttcaaggta
    10321 gcttttccga ttttacgttc tacgccttgt tggtaaaatg gcgacattgc tgccggtgca
    10381 cgatttatta aataaggagc acctaaatct gtaacttctg cttcaaaagt atgacctccg
    10441 gtaaccactt tagcagtagc gccattagca gtagttttaa tatcgaaagt agccggtaat
    10501 acaggtgcct tttcaaatgg cgtacctgca aatatttttg gtccaaagta acctcccact
    10561 ttactgtcag ttgagataaa gcccataacc attggtggtt ttgtactgtc gggttgatat
    10621 aaaatcatcc cactaggagc attgcctaaa ggcgtgctca ccatatgagc cacatgaaaa
    10681 atgacattgg gcattttcag attagggtcg gttactccgg caatatcaat gccgttttcc
    10741 caactcatcc agtctgtaac atttggatca gctttggctt cattagcggt aacaacagca
    10801 tctggtgttg atgcattctc tgtcgattca tttttgggtt tgcaggcaat gaaaaacaac
    10861 attgcggcaa ttgggataat tacttttttc attttgtgtt gattttgtta ttgcaaagta
    10921 tagaaaaaaa tgacgatata catacccaat caagggtatt ctgcttgatt ttcagatttt
    10981 tgttatgcaa tatgttaaaa ttctgcgagt gttgaaattc tttattaatt ttgcaaaaaa
    11041 tattgaatga ataaattttt caagctaacc tacatacttc tttttcttta tacttttaat
    11101 acacttaaaa gtgctaatga actttacatt aacggtcccg gaagtaccac ttctacagcc
    11161 acactttacg acagtacctg tctgattttt gtaaacggtg aaataaagaa taatgacgga
    11221 ttatttatta atcgtggtgg tacgattgaa ttgacaggta attggacaaa tacggtaagt
    11281 agtaataaat atcaaagtac tggtactgaa aaattttatg gcacctcaga acaagtcatt
    11341 agtggaactt taaacggtac aacgtacact actaatgctt atgataatca gttttgtcat
    11401 cttaaagttt acagaaagtc taccataacc acaactcctg caaaatatat ttctttggga
    11461 gctaatgtta acgtagccgg aagtattagt tttgagacat caacaccaaa tacaactggg
    11521 caggaacagg tacaaatg
//
LOCUS       DAOQII010000055        11431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2862_length_11431_cov_5.422732, whole genome shotgun sequence.
ACCESSION   DAOQII010000055 DAOQII010000000
VERSION     DAOQII010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11431
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_11431_cov_5.422732"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLF48_04135"
     CDS             complement(<1..558)
                     /locus_tag="PLF48_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNF48548.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDHFLAYDPQSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQEITREVIRKIGYTKSEYMFEANSCGIFSAIHEQSADI
                     NRGVDRKTGKSFEAHANAQGAGDQGMMFGYATKETDNYMPLALDLAHKILQELSKTRR
                     AGKELKYLRPDAKSQVTIEYDDNNQP"
     gene            766..2778
                     /locus_tag="PLF48_04140"
     CDS             766..2778
                     /locus_tag="PLF48_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNF48549.1"
                     /translation="MNNNLLNPVKKLLVANRGEIAIRIFKTCKKMGIATVAVFSDADE
                     NSLFVEYADEAVRIGGKQPNESYLIADKIIEAAKKTGANAIHPGYGFLSEKATFAQQV
                     IDEGLIWVGPHPKAIEIMGSKIGAKKIMQENGVPTIPGYQGDDQSEATIKVKAIEIGF
                     PVLLKASAGGGGKGMRIVREEKELDKAIAEAKSEALNGFGDDTLLIEKYFDTSRHVEF
                     QIFGDKHGNALHLFERECSIQRRYQKVVEESPSPFISEVTRQKMGEAAVLACKAINYD
                     NAGTVEFIVAPDQSFYFLEVNTRLQVEHPVTEEVVGLDLVRLQIEVAQGNPLPYSQDE
                     IKQSGHAIEVRLYAEDPGNHFLPVTGKIIDWIPDKTKGIRYDTGITIGSEISTFYDPM
                     IAKIIAKGNNRNEAITLLSTALKKTVCSGFTTNKNFLIAVLENEDFRNGNFDTHFLDR
                     QFNYAGEKYSQETLDILTCALVHYRFLQRQEERKLANGVPAGWRNNFYQAQMEKYSYQ
                     GFEFPIAYTNHNSELHISFADKSFVSKVSPQSTVDGRRSTNSLTLEINGVRRNFHISQ
                     NGHQFFVQSPALGQIVLHSVERFPLPQTETVKGGYIAPMPGEVTSVLVLAGDKVKAGD
                     ALLKMISMKMENTIEAHTDGEVEEVYVGNKQFVEAGTLLLKMKEGN"
     gene            3047..3799
                     /locus_tag="PLF48_04145"
     CDS             3047..3799
                     /locus_tag="PLF48_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNF48550.1"
                     /translation="MPEQKNKISLNDKIINLASNNIVLIIILYTSIHIASCGYIGLLK
                     PDKSVYPLTGIDISHHQGIIEWSKAKNEISFVFVKATEGSDFADANFKDNIDSIVKYN
                     IPYSAYHFFTFCSSGKNQAINFINHFKIDSNSLPPVIDLEFLGKCNTRNKDSFNVKNE
                     IKDFLEAVQLHYKKIPIIYTTYEFYNRYLLNDFSTYHFWIRDLFKEPDLKDGKEFVFW
                     QYSNSGNINGIKGNVDMNVFKGNIDKLISLSN"
     gene            3829..4653
                     /locus_tag="PLF48_04150"
     CDS             3829..4653
                     /locus_tag="PLF48_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF48551.1"
                     /translation="MLYTQEQTLNFSEQHFAYLIVEEKENVLTITLNRPEKRNAFHIP
                     LANELAYALAYAHYNNQIWCVVLKANGSVFCAGADLKAFAGDDTTEKPSTIPMPKEMI
                     KLGEEFNGLHKPCIAQVHADVYAGGFLMIGGCTHVIAVESAKFGLPEVKRGIFPFQVM
                     ATLEPLMSARQLLDLCIRAKTLSAQEAKEYGLVTEIVTPENLENSVQQLIEEIKEQSP
                     TAIRLGLKAYHEMKSISADEKHKYLHAMLMQCLQSKDAAEGLAAFKEKRKPEWRGN"
     gene            4737..6128
                     /locus_tag="PLF48_04155"
     CDS             4737..6128
                     /locus_tag="PLF48_04155"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNF48552.1"
                     /translation="MKQTKIAGAQGFYGDSPLGAIQIAMQGEADYLMHDALAELTLSI
                     LQKDKLKDPSMGYARDIEVHARTLYPIAFSKGIKIVTNSGGLNPESAAEKVKAILEKQ
                     GIKGIKIATISGDDVLSRLPEFKEQQVELNNLDDNTSYYDSKYMPTHANVYIGAQKVK
                     EALDNGANLILAGRVADPCLALGILAHEFNWKIDDASTQEDLDRMAFAITIGHILECG
                     GQASGGNAYSEWPMHYSVSDLGYPIAQVNEDCTAILTKAKNTGGKVSRNTIREQLVYE
                     IHDPTTYITPDVVVDLSNIKLEDVGENEVSISGIKGKPRPEKLKLCIGQMEGYVTEQF
                     FFFSYPFAYDKLQMFIKAVKETWAKLPVQILESRFNIIGVNGLHEDAVEIPDADELNQ
                     RSELGLRIAIKHKDDRTGKTAIAGITCLGLNGPPGVISVPGWGNMNRAMLSLFPCLIP
                     RELIEPKVDYVIL"
     gene            6153..6506
                     /locus_tag="PLF48_04160"
     CDS             6153..6506
                     /locus_tag="PLF48_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF48553.1"
                     /translation="MGEKENIILKLTFEFSISIIEFTENLETNRRFVIANQLLKSGTS
                     IGANVKEAQNAESKADFIHKIKIAMKEVEETEYWLELCHYSKNYPNTTDLQTKLKSIK
                     LILAKIITTSKNKKE"
     gene            6544..6858
                     /locus_tag="PLF48_04165"
     CDS             6544..6858
                     /locus_tag="PLF48_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48554.1"
                     /translation="MTVKLINIASARSGDKNNVCNIGVMAKSKEDYATLKKYLTPEMV
                     KAHFKGLIKGKVERFEWDALESLNFVCHEALDGGASRSLRMDTLGKNFSSHLLRLELE
                     IS"
     gene            6900..7277
                     /locus_tag="PLF48_04170"
                     /pseudo
     CDS             6900..7277
                     /locus_tag="PLF48_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            7506..8009
                     /locus_tag="PLF48_04175"
     CDS             7506..8009
                     /locus_tag="PLF48_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNF48555.1"
                     /translation="MILAIDVHYKEPYAKAVGVLFNWQDEHPQTVITAIINEVEEYQS
                     GQFFKRELPCILELIKQVDITSIEAIIIDGHVYVDNQKKYGLGAHLWSVLNGVVPIIG
                     VAKKSFIRTNEVCHPVCRGESKQALYVSVIGDIDMNNAVQHIRLMKGEFRIPKMLKYL
                     DQLTKEV"
     gene            8151..9242
                     /gene="aroC"
                     /locus_tag="PLF48_04180"
                     /pseudo
     CDS             8151..9242
                     /gene="aroC"
                     /locus_tag="PLF48_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
     gene            9248..10162
                     /gene="prs"
                     /locus_tag="PLF48_04185"
     CDS             9248..10162
                     /gene="prs"
                     /locus_tag="PLF48_04185"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNF48556.1"
                     /translation="MKKILFSVQSYHYMKSNMLKMYGHDLEDGLIEIKSFPDGEQYHR
                     LLSNVEGKHCIIIGGTIDDLSTLELFDIAIACEQYGAEKLSLVIPFFGYSTMERAVKP
                     GEIVKAKSRALLFSSIPDTSEQNEILLFDLHTEGLPYYFESNCRAKHIYCKEIVKEIA
                     LEIGGSNFVMASTDAGRAKWVESLANDIGVGAAFVFKKRISGDETQLTGISAEVQDKT
                     VIIYDDMIRTGGSLLKAAEAYKKSGASKIYVITTHGLFINDALNKLKQSGLISRVYAT
                     NTHPNAVAIKDEFLEVRNIEKLIFGNLK"
     gene            complement(10283..10885)
                     /locus_tag="PLF48_04190"
     CDS             complement(10283..10885)
                     /locus_tag="PLF48_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNF48557.1"
                     /translation="MKKIELEVLSITQGFTQHHSYAVVLNEVKGKRRLPIVIGAYEAQ
                     AIAVIIEQIPPVRPLTHDLFKNVLDSFSIFMSEVIITNLMEGVFYSKLVCIFNGEEHE
                     IDSRTSDAIALALRFGAPIYIYEDILDQSGILLVEEETKEDIFEDDVEVALSDIKSQK
                     EKTDYNIYTNKELEKLMNDALHDEDYEKAAKIRDELNKRK"
BASE COUNT         3889 a         1986 c         2268 g         3288 t
ORIGIN      
        1 aggttggttg ttatcatcat actcgatagt tacctgagat ttagcatccg gacgcaaata
       61 ctttaactcc tttcctgctc tgcgtgtttt ggataattcc tgtaaaattt tgtgtgccaa
      121 gtccaaagcc aatggcatgt aattgtctgt ttctttagta gcataaccaa acatcatacc
      181 ttggtcgcca gctccctgtg cattagcatg tgcttcaaac gattttcctg tttttctgtc
      241 caccccgcgg ttaatgtcag cactttgctc gtgtatcgct gaaaagatac cgcatgagtt
      301 cgcttcaaac atgtattccg acttggtata accgattttt cggatgacct cacgagtgat
      361 ttcctgtaca tctaaatagg ctttagattt tacttcacca gctaatacaa cttgtcctgt
      421 tgtaactaat gtttcgcatg ctactttaga ttgcggatca taagccagaa aatggtcaat
      481 caacgcatct gaaatttggt cggctacttt atccggatgt ccttccgata ccgattctga
      541 tgtgaataaa taaggcatat taaatttact ttttaatttt ttagaaaggc gaaaatacga
      601 attaataatt ttatgtcaaa atagtgcgta ttatcaataa gttgctccat tttttcttaa
      661 ttatcggctc tttcttattt tatatgacaa aagaaatgca taatttacga ttctcataaa
      721 cttgctatac tatttactat aattattaaa gaacagacat atacgatgaa taacaaccta
      781 ctgaacccag taaaaaaact ccttgttgcc aaccgcggtg aaatagccat ccgcatcttt
      841 aaaacctgca aaaaaatggg cattgccacc gtagccgttt tttcggatgc cgatgaaaac
      901 agcttatttg ttgaatatgc cgatgaggca gtgcgcattg gcggcaaaca accgaatgaa
      961 tcctatctga tagcagataa aataattgaa gcagctaaaa aaacaggtgc gaatgccatt
     1021 catcccggct atggcttttt gagtgaaaaa gcaacctttg cacagcaagt tattgatgag
     1081 ggattaattt gggtggggcc acacccgaaa gcgattgaaa ttatgggttc taaaatcgga
     1141 gcaaaaaaaa taatgcagga aaacggcgtt cccactattc ccggctatca gggtgacgac
     1201 caaagcgagg ctaccataaa agtaaaagcc atagagattg gatttccggt acttctgaaa
     1261 gccagtgcag gcggaggtgg taaaggtatg cgtattgttc gagaagagaa agaactggat
     1321 aaagccatag ccgaagctaa atcagaagca ttgaatggtt ttggagacga cacattgctg
     1381 atagaaaaat attttgatac ctcacgtcac gtagaatttc aaatctttgg ggacaaacat
     1441 ggaaatgcct tgcatttgtt tgagcgcgaa tgtagtattc aacgccgcta tcagaaagta
     1501 gtagaagaaa gtccttcccc atttatatct gaagtaacac gacaaaaaat gggcgaggcg
     1561 gccgtgctgg catgtaaagc tatcaattat gacaacgcgg gaacggtaga gtttattgta
     1621 gcacccgacc agagttttta ttttctggaa gtcaatacgc gcctgcaagt ggagcatcct
     1681 gtaacagaag aagtagtagg tttggattta gttcgcttgc aaattgaagt ggcacaaggc
     1741 aatccgcttc cttactcgca agatgaaata aaacagagcg gacatgctat tgaagtgcgc
     1801 ctttatgctg aagatccggg caatcatttt ttgccggtca ccggaaaaat tatagattgg
     1861 attccagata aaaccaaagg tatccgctat gatacgggca tcacaatcgg ctcagaaatt
     1921 tctacttttt acgacccgat gattgcgaaa ataattgcca aaggaaacaa cagaaatgag
     1981 gcgattacct tgttaagtac ggcattgaaa aagacagttt gttccggttt tactacaaat
     2041 aaaaacttcc tgattgctgt tttggaaaat gaagatttca gaaacgggaa ttttgatacg
     2101 cactttttag atagacaatt taattatgcg ggagaaaaat acagtcagga aacactcgat
     2161 attttgacat gtgctttagt gcattatcga tttttgcaaa gacaagagga acgcaagctg
     2221 gcgaatggag tgccggcagg ctggcgcaat aatttttatc aggcacaaat ggaaaaatat
     2281 tcctatcagg gttttgaatt tcctatagca tatacgaacc acaatagtga gttgcatatt
     2341 tcgtttgcag ataaaagctt tgttagcaag gttagtccac agtcgacggt cgacggtcga
     2401 cggtcgacga attcgcttac attagagata aatggggttc gccgcaactt ccatatttct
     2461 caaaacgggc atcagttttt tgttcaatct cctgcattgg ggcaaatcgt attgcatagt
     2521 gtggagcgtt ttccattgcc acagacagaa acggtaaaag gtggttacat tgctccgatg
     2581 ccgggtgaag ttacatcagt attagttttg gctggagata aagtaaaagc cggagatgcg
     2641 ctgctgaaga tgatttcgat gaagatggaa aatacgatag aagcacacac cgacggggaa
     2701 gtagaagaag tgtatgttgg caataaacag tttgtagaag cagggacttt gttgcttaaa
     2761 atgaaagagg ggaattagat aatttggaaa tgtgatgatg taaaaatgtg ttgatttacc
     2821 aatataccaa tggagttgat ctggtaattt taaatcgaga cgatgagaat gcttggttat
     2881 aaactgatgg ataaatgaat ctaatttcta ttttatataa atttaaataa tgaaacagac
     2941 acaaccagtc ggcattatta tcgttcagaa aagaatgaat tttccagaca gtgtctggaa
     3001 aattcatgtt ataaaataat aaatttaata gtatagcaat tcaataatgc cagagcaaaa
     3061 gaataagata tcattaaacg acaaaataat aaatcttgct tccaacaata tagttctaat
     3121 aattatatta tatacatcta ttcatatagc atcttgtggc tatattggat tattaaaacc
     3181 agataaatcc gtctatccat taaccggaat tgacatatca catcatcaag gaattattga
     3241 atggagtaaa gcaaaaaatg aaattagttt tgtatttgta aaagcaacag aaggttcaga
     3301 ttttgcagat gcaaatttca aggataatat tgattctatc gtgaaataca atattccata
     3361 ttctgcctat cattttttta cattttgcag ttctggtaaa aatcaagcta tcaattttat
     3421 aaatcatttt aaaattgata gtaattcatt acctcctgtt atagatttag aatttttagg
     3481 aaaatgtaat acaagaaaca aggatagttt taatgtcaaa aatgaaatta aagatttttt
     3541 agaagctgtt caattgcatt ataagaaaat acccattatt tatacgacat acgaattcta
     3601 taataggtat cttttaaacg atttcagtac ctatcacttt tggattcgag atttatttaa
     3661 agaacccgat ttaaaagacg gtaaagaatt tgtattttgg caatattcca attcagggaa
     3721 cataaatggc attaaaggta atgttgacat gaatgtattt aaaggaaata tagacaaact
     3781 catatcatta tcaaattaac acatttttca aattttcaaa ttacaactat gctatacaca
     3841 caagaacaaa ccttaaattt ttcagagcag cattttgctt atcttatcgt cgaagaaaaa
     3901 gaaaatgtat taacgattac gcttaatcgc cccgagaaac gtaatgcctt ccacattcct
     3961 ttagctaatg aactggctta tgcattggcg tatgcacact acaacaatca aatctggtgc
     4021 gtggtgctca aagccaatgg gtcggttttt tgcgccggtg cagatttaaa agcatttgcc
     4081 ggcgatgata ctacagaaaa gccgtccact attccaatgc caaaggaaat gataaaactc
     4141 ggtgaagaat tcaacggctt gcacaaacct tgtattgcac aagtacatgc agatgtatat
     4201 gccggtggat ttttgatgat aggcggttgc acgcatgtta ttgccgtaga atctgctaaa
     4261 ttcggtttgc cggaagtaaa acgcggtatt tttccatttc aggtaatggc aacattagaa
     4321 cccttaatgt cagcccggca attattggat ttatgcatac gggctaaaac attgagtgcc
     4381 caggaagcta aagaatatgg cttggttacg gaaatcgtta cacccgagaa tttagaaaac
     4441 agcgtgcagc aattaataga agaaatcaaa gaacaatcgc cgactgccat tcgcctgggc
     4501 ttaaaagcct accacgaaat gaaatccatc tcagcagatg aaaaacacaa atacttacat
     4561 gccatgctga tgcaatgcct acaaagcaaa gatgctgccg aaggcttagc cgcattcaaa
     4621 gaaaaaagaa aaccggagtg gaggggtaat taaataatgt aacaatatac caatgtatca
     4681 attgagaaat aaaatattgc tacatcggta catcaatcaa ttggtaaatt tttattatga
     4741 aacaaacaaa aatagcaggc gcacagggtt tttatggcga ttcgccgctt ggtgccatcc
     4801 agattgccat gcagggcgaa gccgattatc tgatgcatga tgccttggca gaactcactt
     4861 tatctatctt acaaaaagat aaactcaaag atccgagcat gggttatgcc cgagatattg
     4921 aagtacatgc ccgtactctg tacccgattg ccttttcaaa aggaataaaa attgtaacca
     4981 acagtggtgg attaaatccg gaaagtgccg ccgaaaaagt aaaagccatc ttagaaaaac
     5041 aaggtataaa aggcatcaaa atcgccacta tttcgggtga tgatgtattg tctcgcttac
     5101 ctgaatttaa ggagcagcag gtagaattga ataatttgga cgacaatact tcatactacg
     5161 attctaaata tatgcctacg catgccaatg tctatatcgg tgcgcagaaa gtaaaagaag
     5221 cattagacaa tggtgccaat ttgatattag caggtcgtgt tgccgaccca tgtttagctt
     5281 taggaatttt agcgcatgag ttcaattgga aaattgatga tgcatccact caggaagatt
     5341 tagacagaat ggcatttgct attactatcg ggcatattct ggaatgcggc ggacaagcaa
     5401 gcggtgggaa tgcctactcc gaatggccta tgcattacag tgtttccgac ttaggttatc
     5461 ccatcgctca ggtaaacgaa gactgtactg ccattttaac caaagcaaaa aacacaggcg
     5521 gaaaagtttc cagaaatacc atcagagaac aactggttta tgagatccat gaccccacca
     5581 cgtatatcac acccgatgta gtagtcgatt tatccaacat aaaattagaa gatgttggtg
     5641 aaaatgaagt cagtatttcg ggtataaaag gcaaacctcg tcccgaaaaa ttaaaactat
     5701 gtatcggtca aatggaagga tatgtcaccg agcaattttt ctttttctct tatccttttg
     5761 cttatgataa attacaaatg ttcatcaaag ctgtaaaaga aacttgggca aaattgccgg
     5821 ttcagatttt ggaatccaga tttaatatca ttggcgtaaa cggtctgcat gaagatgccg
     5881 tcgaaattcc ggatgctgat gaattaaatc agcgaagtga attaggattg cggattgcga
     5941 taaaacacaa agacgacaga accggaaaaa cggccatagc aggcatcact tgcttaggat
     6001 taaacggacc tcccggcgta atctctgtac ccggctgggg caatatgaac cgcgcgatgc
     6061 tgagtttatt tccatgtctg atacccagag agctcataga gcccaaggtg gattatgtaa
     6121 ttttataaat gtactgatgt agcaatttac caatgggaga aaaagagaat ataatcttaa
     6181 aactaacttt tgaattttca atttcaatca ttgaattcac agaaaatttg gaaacgaaca
     6241 gaagatttgt aattgcaaat cagctgttaa agtcaggaac gtcaattgga gctaatgtaa
     6301 aagaagctca aaacgcagaa agcaaagcag actttattca taaaataaaa atagcaatga
     6361 aagaagttga agaaaccgaa tattggcttg agttatgcca ttattctaag aattatccaa
     6421 acacaacgga cttgcaaact aaattaaaat caataaaatt aattttagca aaaatcatta
     6481 ctacatcaaa aaataaaaaa gaatagtaat tgatacattt ccacatcttt aaattgatac
     6541 attatgacag taaaactaat aaacatcgca tcagcgcgta gtggcgacaa aaacaatgta
     6601 tgcaacattg gtgtaatggc aaaatctaaa gaagattatg caacactgaa aaaatactta
     6661 actcctgaaa tggtgaaagc gcacttcaaa ggattaataa aaggcaaggt agaacgcttt
     6721 gaatgggatg ccttggaatc gctcaacttt gtttgccacg aagctctgga tggtggtgca
     6781 tctagaagtt taagaatgga tacccttggc aaaaacttta gctctcattt attgagattg
     6841 gaattggaaa ttagttaacc gcaaaggttt caaagaattt cgcaaagaac acaaaggata
     6901 tgaaagaaga tgaattatca catattatta taggtttaag tattgatgtt cataatgcat
     6961 taggtcccgg attattagaa agtgcctatc aagaatgtct ttattataaa ttaaaaaaag
     7021 aaggtttatt tgttgagaaa gaaaagccaa tgccaaattc ttgagaagaa acaaaattag
     7081 attgtagata cagaattgac ttattagtag aaaataaagt tgtaattgaa attaaaagtg
     7141 ttgacacctt aaatgatatt catttagcac aaacattgac ctatttaaaa ttaggaaact
     7201 acaaacttgg attgctaata aactttaacg tagtcttatt aaaacaagga attaaaaggg
     7261 tagtaaacgg attatagata ttggtaagct ttgcgaaaaa acttagtgcc ctttgcggtt
     7321 aaaaaaaaat gaatatttta gaaaatatta ttttatataa tgacataaag tattccaaaa
     7381 aattctgtca accttgtctt tatttttatt acacaagaaa aaatttaagg ctggtgtttt
     7441 agaaacggaa tggagatgca agagctacaa cataagagaa ataagataaa agcagaagaa
     7501 taactatgat attagccatt gatgtacact acaaagaacc ctatgcaaaa gctgtagggg
     7561 tattattcaa ttggcaagat gagcatcctc aaacagtaat tactgctatc atcaatgaag
     7621 tagaagaata ccagtcagga caatttttta aacgcgaatt gccttgtata ctggaactaa
     7681 tcaaacaagt tgatatcact tcaatagaag caattataat agatggtcat gtgtatgttg
     7741 acaaccaaaa gaaatatggc ttaggtgcgc acttgtggag tgtactaaat ggagttgtgc
     7801 cgattattgg agtagcaaag aaatcgttta tccgtacaaa tgaagtctgt catcctgttt
     7861 gtcgtggtga aagtaaacag gctttgtatg tatcggtaat tggtgacatt gatatgaata
     7921 atgcagtcca acatatccga ttgatgaaag gagaattcag aataccgaaa atgctcaaat
     7981 atctggatca gcttacgaaa gaagtttgat gagaaaactt atacccagta ttttgctccg
     8041 tttacgaagt aaaaagacta aacattccaa taaaacaact aacgtatttt ttcatttttc
     8101 gcccatttcc cgaatcttcg atttcatgat ttaatcgtat ttttgcaggt atggcaggta
     8161 atgttttcgg cgagttgttt cgcattgcta cctttggcga aagccatggg gcagctatcg
     8221 gtgtagttat tgacggctgt cctgccggac taactattga tactgcattc atccagtcag
     8281 agttggacaa gcgcaaaccc ggtcagtctg ccattgttac ccaacgcaaa gaagcagata
     8341 cgtttgagat tttatccggt atttttgaag gcaaaacaga aggcacaccc attgctctct
     8401 taatccgaaa tgaagatcaa aaatcgaaag actatgagca tatcaaagac agttttcgcc
     8461 cttcacatgc cgacttcact tatcatgcaa aatatggctt tcgagattat cgcggtggcg
     8521 gaagaagttc tgcacgcgaa actgcagccc gcgtggctgc tggtgctatt gccaaacaat
     8581 tattggcaca attcggtatc gaagtttttg cttatgtatc acgagtgggt gcgattcaac
     8641 tcaacaaaaa ttacacgcaa ctcgatttac acgctataga aaataatata gtacgatgcc
     8701 ctgatgcaga tacagcagaa caaatgattg cacgcattca agaggtgcgc aaggcaggcg
     8761 atactattgg cggcatcgtt tcatttttgt gaaaaaatgt acccgccgga ttgggtgagc
     8821 ccgttttcga caggctacac gccgatttag gcaaggccat gctgagtatt aatgcctgca
     8881 aaggatttga gtatggaagt ggctttgcaa gtttacattt attgggcagc gagcacaatg
     8941 atttgtttta taatgacaat ggtgtaatta aaacaaagac aaattacagc ggaggaatac
     9001 aaggcggcat cagcaatggc atggatatct acttcaatgc agcttttaaa ccggttgcta
     9061 ccatcatgca ggcgcaggaa agtgtggatc aggatggcaa tactgtagaa gttacaggca
     9121 gaggcagaca tgatccttgt gtattgccaa gagccgtccc tattgtttct gctatggccg
     9181 cattagtcat tgcagatcat ctgctgcgca gcaaagtcaa tcgtattgac caattaatct
     9241 gaaacctatg aagaaaatat tgttttccgt tcagtcttat cattatatga aaagcaatat
     9301 gcttaaaatg tacgggcatg atttggaaga cggattaata gagataaaat catttcccga
     9361 tggtgaacaa taccatcgtc ttttatctaa tgtcgaaggc aaacattgta ttatcatcgg
     9421 aggcacaata gacgatttat ctaccttaga attgtttgat attgccattg cctgcgaaca
     9481 atacggagcg gaaaaattga gtttagtcat tccctttttc ggatatagta caatggagcg
     9541 tgctgtgaag cccggcgaaa ttgtaaaagc caaatccaga gccttattgt tttcctctat
     9601 tcctgatact tctgaacaaa atgagatttt gttattcgat ttgcataccg aaggattgcc
     9661 gtattacttt gaaagcaatt gcagagccaa acatatttac tgcaaagaaa tcgtgaaaga
     9721 aattgccttg gaaattggag gcagtaattt cgtgatggca tctaccgatg caggacgagc
     9781 aaaatgggtg gagtctttgg cgaatgatat aggagtggga gctgcctttg tttttaagaa
     9841 aagaatttcg ggtgatgaaa cacaactcac cggcatctct gcagaagtgc aagataaaac
     9901 agttattatt tatgatgata tgatacgcac tggaggcagt ttgctgaaag cagctgaagc
     9961 ctataagaag tcgggggcat caaaaatata tgttattacc acacatggtt tgtttataaa
    10021 tgatgcacta aataaactta aacaatcggg gttaatttcg agagtttatg ccactaacac
    10081 tcaccctaat gcagtagcca taaaagatga gtttttagaa gtcagaaata tcgaaaaatt
    10141 gatatttggt aatttaaagt aaagtaatta catttttgac caaaatcaaa gattataagg
    10201 gtgaaacaga actaactttg tattaagaaa agtttagata cataaaaccc taaaaaaata
    10261 gtcgcttctt atagcgacta ttttattttc ttttattcag ttcatccctg attttagcgg
    10321 ccttttcata atcttcatca tgcagggcat cgttcatcag tttttccaat tctttgttgg
    10381 tatagatatt ataatcagtt ttttcttttt gtgattttat atcgcttaaa gcaacttcta
    10441 cgtcatcttc aaaaatatct tcttttgttt cttcttccac caataaaatt cccgactgat
    10501 ccaaaatgtc ttcatagata tatattggtg cgccaaaccg caaagccagt gcgatggcat
    10561 ccgaagtacg agaatcaatc tcatgttctt cgccattgaa aatacaaact aatttagaat
    10621 aaaaaacacc ttccatcagg ttggtgatga tgacttcact catgaaaata gaaaatgagt
    10681 cgagaacatt tttgaataaa tcatgcgtta gcgggcgcac tggtggaatt tgttcaatta
    10741 ttacagctat agcttgcgcc tcataagcac caatgacaat aggcagtctg cgtttaccct
    10801 ttacttcatt cagaacaacg gcatacgagt gatgctgggt aaatccctgc gtgatactca
    10861 atacctccag ctctattttt ttcatcacac aaatatacaa agctgtatta aaaataatac
    10921 tttattttag gcttaatcac tcaatatcgt taagttaagc caaaagactc aagaagttag
    10981 aaatagttaa cctcccccta ccccctccta aaaataggag ggggaattaa agggagtggt
    11041 tttttatgtc tgaaaaatgg taatgttaaa tataatatat gtttttagat ttaaatttta
    11101 gaaatagttg acctccccca cccccctcct aaaaatagga gggggaatta aagggggtgg
    11161 tttctgataa aaaatcaaat tcaatggtaa ataaggattg caagaataat gtggttaatt
    11221 ttatctaaga agctcaagaa gtagaaatag tttacctccc ccacccccct cctaaaaaaa
    11281 aggaggggga attaaaggtt ggcatatctt aaaagtattt aaactaaata taattttatt
    11341 ttgccttaag tgtacaagaa agcccgcaga agagttaggt ttgtgaaagg cacagcaata
    11401 gaagtaaaat caaaaatcag ctaagccata g
//
LOCUS       DAOQII010000056        11360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2893_length_11360_cov_4.571517, whole genome shotgun sequence.
ACCESSION   DAOQII010000056 DAOQII010000000
VERSION     DAOQII010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2893_length_11360_cov_4.571517"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLF48_04195"
     CDS             complement(<1..718)
                     /locus_tag="PLF48_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48558.1"
                     /translation="MKKTYLVLICMLLSYLGFAQTIPFADSKIATKGFSIEKSSPELD
                     INNSFIYGSNFLSLKKGDAVYVTCKENEGENIEFYLREKGSKNLLAQVSESANAWFYY
                     KADKDVTLEVFGIIGKNKYLSNPKQCTIKYLQGNASDYYYSDKDNIPQKILALAYGKP
                     LAFSNICYHRNTRINKRDTTFYNIFKQGDNMRVFGNTIEYSIENGVSKSKAAALIASW
                     KGYIDKAFQNVTLEDKQVYNE"
     gene            859..1383
                     /locus_tag="PLF48_04200"
     CDS             859..1383
                     /locus_tag="PLF48_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48559.1"
                     /translation="MNKLIIVLSVVCFIFTTSCKKDNNANNFDSNLTNGRCRVFCTIN
                     GAVTMDYASQDVNSFSAYNAVFMALAPDFRNTSSGERAIMEILIPISTTPGSYNLTDA
                     GAPNISFTFTKDNMYSGTGNSWKAIVGSNMTFVLTKSSYTELEGTFSGTAVSDADGST
                     VTITNGKFKTVFTH"
     gene            complement(1416..2309)
                     /locus_tag="PLF48_04205"
     CDS             complement(1416..2309)
                     /locus_tag="PLF48_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48560.1"
                     /translation="MLKRFSFFLISVLLISSCAMNKLFYQPFKIPAKTTHVVINKGKP
                     DSVQVRFIGNVHQPVFLKNSTDMLQFPYTIESILFKSTDGNMLNGWMLKPRDGNVQGT
                     ILHLHGNGGMLLSQYEAIAPLTQYGYQIFMFDYSGYGFSSGKPTRANVLADAQAALTY
                     LLSRKDVQANEILLYGQSLGGHLAAVLAEQQQESIQALVVEGAFSSHKDVAQHFAGKF
                     GRAFVKDGYSAKVALKTYSKPLLVIHSINDKTIPYAMGKTLFQVANEPKYFYTVEGDH
                     IHAVIDHADSIAYKIKFMTDK"
     gene            2497..2973
                     /locus_tag="PLF48_04210"
     CDS             2497..2973
                     /locus_tag="PLF48_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HNF48561.1"
                     /translation="MKNLCIILSVFATFSLLSCKKDSSSSLAAGKSRISLDASGATSG
                     SFASDDRVSTVAKSGSIINISGASLSADMVMMLLPATITTGTYDLSTLSSSAAGPTFS
                     YSKGANGWAAVPGDHFTIVVTKATSTEIEGTFSGTAHNDDAGTDVTISNGKFAAKY"
     gene            complement(3041..4168)
                     /locus_tag="PLF48_04215"
     CDS             complement(3041..4168)
                     /locus_tag="PLF48_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNF48562.1"
                     /translation="MRVYLDNAATTPLSKEVLDAMMPYFTEHFGNPSTTYSYGRDTRA
                     AIETARKTIASLLNAQPGEITFTSGGTESANMAIKGAVDYLGVERIITSPIEHHCVEY
                     AVDYCRDYKKTEKVLIQTDNAGNIDLAHLENLLKSSSKKTLVALMHANNEIGTMLNLK
                     QVAEICKANQALFFSDTVQSFAHHIIDTKEIPVDFVTGSAHKFHGPKGSGFLYMRKQN
                     KVQALIHGGGQERGFRAGTENVAGIVGLAEAAKMAYNTLEEDKKHILSLKYYFKQQLL
                     DIFEDIDFNGNTDERSLFTVLNVSFPPHENAPLLLFHLDLAGICCSGGSACNSGAASG
                     SHVIQALNKNPDRVSIRFSFSKHNTKEELDYVLEKLKEIFK"
     gene            complement(4392..4601)
                     /locus_tag="PLF48_04220"
     CDS             complement(4392..4601)
                     /locus_tag="PLF48_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48563.1"
                     /translation="MLKEYTIEIKSVKAPPISLAMCDSINCPICDIDLSVENKIDLST
                     FEVNEDTIIYCEGCDLNLRFKIAKI"
     gene            complement(4595..5365)
                     /locus_tag="PLF48_04225"
     CDS             complement(4595..5365)
                     /locus_tag="PLF48_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HNF48564.1"
                     /translation="MNNKGNKHKNYEILNLLGYGLAKFDNEFIKQFGFKTKHSFFEYF
                     VKIGIVETGSVVKNRMDLFDPFFENSRKGWWQKGDAYIHRKLLIDSLFGNESAVEYSN
                     IVKLFLKDNFKIKDIFVEVKPIVKSRFKKLQETGLEAELYFINNFNSIDIFKNGIIED
                     ARLYGDGYDFQISVNNNSFLAEVKGIRAKKGRFRLTENEYLKAKEYKHDFIVTLVLNL
                     DDLPKFLTIENPLKNLKFEERLIKSKEVREYHLINDLC"
     gene            complement(5601..6752)
                     /locus_tag="PLF48_04230"
     CDS             complement(5601..6752)
                     /locus_tag="PLF48_04230"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF48565.1"
                     /translation="MNFIHPDIFYTRLKTHHKLLYLHLVKHFSIRNFLIGIAYFIVIF
                     IVSSFNAICRLLDELLFPNYHQVEIHQPVFIISNPRSGTTFLHRLMCLDDKKFVYNLL
                     YHTTFHSIIFYRAVQIISGIDKKIGGPLHWLLDKINDTMFKGWDEIHKTGFNKSEEDE
                     GWHFISGISPSIGLITPYLKSFNELYIPDNLSEKEKNNIKKYYKASIQRWMYALGSDK
                     IFLSKTVMSSGRLEMLHDIFPDVKIIFLIRHPYQAIPSFTSMFAEPWSTLYPSIPSNS
                     EAYREWGEVGIEYYKYFNIEKSKFKQENFITIPYTELVADPMATVLKIYNQLQLEITP
                     EFTARLSEETHKQRKYTSKHSYSLEEYGFEKEYIYQQLHFIFEEFGFEK"
     gene            complement(6822..7196)
                     /locus_tag="PLF48_04235"
     CDS             complement(6822..7196)
                     /locus_tag="PLF48_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48566.1"
                     /translation="MKTLLVTLLFVSSNIMHGAEVYLCKTVKKGIPVEAQTTWDAKKD
                     NICIVFNHDKPLQNNAVYKLSIARRVEKYYFWRFSNKDIFVKKGQSQIVTHSVIQEPG
                     DYIISIIDNNGYILAEQSCTIK"
     gene            7481..8053
                     /locus_tag="PLF48_04240"
     CDS             7481..8053
                     /locus_tag="PLF48_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF48567.1"
                     /translation="MYLFFDTETTGLPKDWKAPLSKLDNWPRMVQLAYMLYDKEGNKL
                     QEADLIIKPEGYTIPKEAADVHGITTDIANQKGIPLELALASFYEAAQTAEVLVAHNM
                     AFDEMIVGAELLRKNKHNIIPIKKRICTMQSTVNFCKIPSKYGFKWPKLTELHYKLFD
                     TDFVDAHNAYVDISITAKCFWKMKAMGIID"
     gene            8091..8897
                     /locus_tag="PLF48_04245"
     CDS             8091..8897
                     /locus_tag="PLF48_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNF48568.1"
                     /translation="MIHQIIKDKSTKLFIFLGGFFIANAFIAEVIGVKIFSLEKTLGI
                     SPLQIPIFGNDFSFNLTAGVLLWPVVFIMTDIINEYYGERGVRFLSYLTAGLLAYAFF
                     MYYLAIQLVPADWWPASKADVGVNNMQSAYAQIFGQGLAIILGSLIAFLIGQVVDVVI
                     FHKIKKWSGEKYIWLRSTGSTVVSQFIDSFVVLFIAFYLYPKYISESNGKAWPFYVVM
                     SVCVGNYIYKFFMAVLLTPVIYWVHNGIDKYLGKNLATEMKQAAMADEIQ"
     gene            complement(9048..10766)
                     /locus_tag="PLF48_04250"
     CDS             complement(9048..10766)
                     /locus_tag="PLF48_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNF48569.1"
                     /translation="MNKTYNINLAGQIFAINDDAYESLSAYLQSLRQFYKNEIDRDEI
                     IKDIESRFAELFLAKGKGYIVTKKDTDEVIGMMGRPQEFDTSYTEDDIEIEPNKTPYQ
                     NAGYETKKRVYRDPDDKIVAGVCSGLAKYFGIDDPIWFRIGFVLITLFTGLGFLVYPL
                     LWLIIPEATTSSQKLTMRGENVNLSSIEKTVREEPVQRQGAGNQILEFIGGFIKLFFK
                     SLLYLGVFVLGIVAVAIIISLLATFFGLGIALVAGNPLLTKYFFDNNSVSWLIGIGGL
                     LATVIPIILFVLAIVHIFSKTNKPLKKNILFSLIGLFLFGILLLNIVYSKGKKLIAEH
                     QKITQSVPLYEASKIDTLDLSMAFTEKKDNIDGVQINSFQDFMNFIARKDEINIPVDI
                     KILPTNSDSFIVVREFSANGKDEKTALENATDITHNIIQKGNQLIIDPYIGIDIPKSK
                     FRNQKLIVKIYVPEGKKIKWNERVEDFMDEDKLDINWSNAGNHTNEISNLKIQLNTLK
                     DSTNINIHIDSNNPDLQEAIQEVQDQLNDKQDELNDKLDDLIDAKISGEHYLFKMHNG
                     ELFPLD"
     gene            complement(10786..11160)
                     /locus_tag="PLF48_04255"
     CDS             complement(10786..11160)
                     /locus_tag="PLF48_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNF48570.1"
                     /translation="MTTTSSSTFNTENTKVQMRKGILEFCILGIISRGEVYASDILDS
                     LKAANILVVEGTVYPLLTRLKNAGLLTYQWKESTSGPPRKYFTLTGTGYRAYDELKET
                     WNELSDAVVKTTDIKSNQNPTI"
BASE COUNT         3410 a         1989 c         2012 g         3949 t
ORIGIN      
        1 gttcgttgta aacttgtttg tcttccaatg ttacattttg aaaagccttg tcaatataac
       61 ctttccacga agcaataagt gctgctgctt ttgatttgga tactccattc tcaattgaat
      121 attcaatcgt gtttccaaat acacgcatat tatcaccttg cttgaaaata ttgtaaaatg
      181 tcgtatcgcg tttgtttatt cttgtatttc tatgataaca gatattggaa aaggctaaag
      241 gtttgccata agccagagcc agtatttttt gcgggatatt atctttatca gaataatagt
      301 aatcggaagc gtttccttgc agatatttta tggtgcattg ttttggattg gacaagtatt
      361 tgtttttgcc aataatgcca aatacttcca acgtaacatc tttgtctgcc ttgtaataaa
      421 accaggcatt ggcactctca ctcacttgag caagcagatt tttacttcct ttttctctta
      481 gatagaactc tatattttca ccttcatttt ctttgcaagt aacatacacg gcatcgcctt
      541 tttttaacga taaaaagtta gaaccgtaaa taaagctgtt attgatgtcc aattccgggc
      601 tggatttttc aatgctaaat cctttagtag ctatttttga atcggcaaag ggtatcgttt
      661 gtgcaaaacc caaataggaa agcaacatgc agattagcac taagtacgtt tttttcatca
      721 gataatcatt tttttcaatt taaagataat tgaaagtcaa acaacattta taccaatttt
      781 agggtattaa taaaaaatca gtactgctac tgctattcgt aattattact ttttgtattt
      841 ttacttaaaa taattcttat gaataaacta attattgtat tatctgtagt ttgctttatt
      901 tttacaactt cctgtaaaaa agataacaat gcaaataatt tcgatagtaa tttaacgaat
      961 ggcagatgca gggtgttttg tactattaat ggagctgtta caatggatta tgcttcacag
     1021 gatgtgaata gctttagcgc atataatgct gtatttatgg ctttagcacc tgattttaga
     1081 aatacctcta gtggtgaaag agcaattatg gaaattttaa ttccaatttc aactacacca
     1141 ggtagttata atttaactga tgcaggtgca ccaaatatta gttttacatt tactaaagat
     1201 aatatgtact caggcactgg taattcatgg aaagctatag ttggcagcaa tatgactttt
     1261 gtgcttacaa aatcaagtta taccgaatta gaaggtacat ttagcggcac tgccgtgagc
     1321 gatgccgacg gttcaactgt tacaataacc aacgggaaat ttaaaacagt ttttacgcat
     1381 taaaagaact ataaaattga tattgttttt tgctattact tatctgtcat aaatttaatt
     1441 ttataagcaa tgctatctgc atggtcgatt acagcatgta tatggtcgcc ctctactgtg
     1501 taaaaatatt tgggttcatt ggctacttga aataaggttt tacccatggc atacggtatt
     1561 gttttatcgt tgatactatg aattactaat agcggtttag aataagtttt taaggccacc
     1621 tttgcgctat atccatcttt tacaaaggca cgcccaaatt ttccggcaaa atgctgagca
     1681 acatctttat gtgatgaaaa tgctccttca accactaatg cctgaattga ttcttgttgc
     1741 tgctcagcca aaactgctgc caaatgccca cctaaggatt gaccataaag caagatttca
     1801 ttcgcttgta catctttgcg gcttaataag tatgttaatg cagcttgtgc atcagcgagt
     1861 acatttgctc ttgtcggttt tccggatgaa aatccatatc cactgtaatc aaacatgaaa
     1921 atctgatagc catattgtgt taacggggct atagcttcgt attgacttaa taacatgcca
     1981 ccatttccat gtaagtgtaa aatggtgcct tgtacgttgc catcacgggg tttcagcatc
     2041 cagccattca gcatgtttcc atctgtactt ttaaataaaa tactctcgat agtataggga
     2101 aattgaagca tgtcagtgct gtttttcaaa aatacaggct gatgcacatt cccgataaaa
     2161 cgtacttgta ccgaatctgg ttttccttta ttaattacaa catgtgttgt cttggcaggt
     2221 attttaaaag gttgataaaa cagcttgttc atggcacaag agctgattaa taaaacagaa
     2281 atcaaaaaaa atgagaatcg tttaagcata gttttctaat gaagtttaag ctgaaattgc
     2341 aaatatacca cccttatttg cttattgtaa attatctttt taaggcttta tttttaaata
     2401 ccgcgaaata cgtatataaa atccgtattg gtacggatat ttaacagtag tgttcttata
     2461 tacatttgca gtattcttaa tcaaaaaaat aatatcatga aaaatctttg tatcatttta
     2521 tctgtctttg caaccttttc tttattgagt tgtaaaaaag acagcagcag cagtttggca
     2581 gccggtaaaa gcagaatttc tttagatgcc agtggtgcaa catcaggcag ttttgcctca
     2641 gacgacaggg tgagcacggt tgcaaaaagc ggctcaatta tcaacatcag tggtgcatca
     2701 ctaagtgcag atatggtgat gatgttgctt cctgctacca ttaccaccgg aacgtatgat
     2761 ttgagtacac tctcttcctc agccgcagga ccaacattta gttatagcaa aggagcgaat
     2821 ggctgggcag ctgttcccgg agatcatttt accattgtgg ttactaaagc cacctcaact
     2881 gagattgaag gtacttttag cggtacggct cataatgatg atgcaggaac tgatgttacc
     2941 atcagcaacg ggaaatttgc tgcaaaatac taatcaaaaa ccagaacaat tataagtaga
     3001 gcatccggaa acgggtgctt tttgttttat ttaaaagtta ctatttgaaa atctctttca
     3061 gcttttcgag tacataatcc aattcttctt tcgtattgtg ttttgaaaat gaaaaacgaa
     3121 ttgaaactct gtcgggattt ttattcagtg cctgtatgac atgtgaaccc gatgctgctc
     3181 cgctgttaca tgcactgcca ccgctgcaac atatccctgc aaggtctaga tggaaaagca
     3241 ggagaggagc gttttcgtgt ggaggaaagg aaacattcag cacggtaaat aaacttcttt
     3301 cgtccgtatt gccattgaaa tctatatctt caaatatatc taacagttgt tgtttgaaat
     3361 aatacttcaa gcttaaaata tgcttcttat cttcttccag cgtattataa gccatttttg
     3421 ccgcttccgc caagcccacg atgccggcca cattttcagt gcctgctctg aaacctcttt
     3481 cttgtccacc gccatgtatc agtgcctgca ctttgttttg tttgcgcata tacaaaaatc
     3541 cgcttccttt aggcccgtga aatttatgtg cagatcccgt tacaaaatca accggaattt
     3601 ctttggtatc gatgatatga tgtgcaaatg attgtaccgt atcggagaaa aacaaagctt
     3661 gattggcttt gcaaatttcg gcaacctgct tgagattaag cattgtcccg atttcattat
     3721 tggcatgcat cagcgccact aaggttttct tgctggaaga ctttagcaaa ttttccaaat
     3781 gcgctaaatc gatattgcca gcattatctg tttgtatcag tactttttcc gtttttttat
     3841 aatcgcggca atagtcaacg gcgtattcca cacaatgatg ctcgatagga gaggtgataa
     3901 tgcgttctac ccccaggtaa tcaacagcac ctttaatagc catgtttgcc gactccgtgc
     3961 cgcccgaagt gaaggtgatt tcgccgggtt gtgcattcag gagcgaagca atcgttttgc
     4021 gggctgtttc tatcgccgct ctagtatctc tgccgtacga gtaagtagta gaaggattgc
     4081 caaaatgttc tgtaaaataa ggcatcatcg catccagtac ttctttactc agcggagtgg
     4141 tggcagcatt atctaaataa acacgcatat tcggagttga gtacaaattt aacgaaactt
     4201 cccgaaaaga aaagagcgca acacgatagt tcaattgttt gagtgagcgg tgctatgatg
     4261 gttagaggta ttattcatca tcttgtggta taggttcgtt tttcataaat gcttcaaatg
     4321 agccttccac gcatttaatc aaacttttct tcaacttctt ttgattgata agagtattat
     4381 ggatgctttc attagatttt tgcaatttta aatctaaggt ttaaatcaca tccttcacag
     4441 taaatgatag tatcttcgtt tacctcaaat gtacttaagt caattttatt ttcaactgaa
     4501 agatcaatat cacaaattgg acaatttatg gaatcacaca ttgcaagact tattggcgga
     4561 gccttcactg attttatttc tattgtatat tcttttaaca taaatcattt attaaatgat
     4621 actctctcac ttctttggat ttgattaatc tttcctcaaa cttcaaattt tttaatggat
     4681 tttcaatagt aagaaatttt ggtaagtcat ccaaattcaa aaccaatgta acaataaaat
     4741 catgcttata ttctttagct ttcaagtatt cattttctgt taatctaaat cttccttttt
     4801 tagctcgaat accttttact tcagctaaaa aagaattgtt atttacacta atttgaaaat
     4861 cgtaaccatc gccatataat cgagcatctt ctataattcc atttttgaaa atgtctatag
     4921 aattgaagtt gtttataaaa tacaattctg cttctaatcc tgtttcttga agttttttaa
     4981 atctcgattt tacaataggt tttacttcaa caaaaatgtc tttaatttta aaattatctt
     5041 tcaaaaataa tttcacaata ttagaatatt caactgcact ctcatttcca aataagctgt
     5101 caatcaataa ttttctatga atatatgcat cgcctttttg ccaccagcct tttctgctgt
     5161 tttcaaaaaa cgggtcaaac aaatccattc tgttttttac aacacttcct gtttcaacta
     5221 ttcctatttt tacaaaatat tcaaaaaatg aatgtttggt tttaaaacca aattgtttga
     5281 taaattcatt gtcaaattta gctaatccat atcctaataa attcaggatt tcataatttt
     5341 tgtgcttatt tcctttgtta ttcatataat taatcgataa acctatagct ttgaaaagca
     5401 aaacactcca ttgacattat tttagccaca atttacaaaa taatttgact ttaatgtttt
     5461 tcctcaattt gattatttac agattggtca atggaaatca gtgttcttat tgattttaaa
     5521 caaaccaaat tcaccttatt tatttacatg aacttaggta tgggttcatt tttgataaaa
     5581 aacagcaaga taaccagttt ctatttctca aacccaaatt cttcaaaaat aaagtgcaat
     5641 tgttgataga tgtattcttt ctcgaagcca tattcttcta aggaatagct atgtttgctg
     5701 gtatattttc gttgcttgtg tgtttcctct gacaagcgtg ccgtgaactc cggtgtaatt
     5761 tcgagttgga gctgattgta aatttttaaa acagttgcca tggggtcggc taccagttct
     5821 gtatagggta tggttatgaa gttttcttgt ttgaatttgc tcttttctat attgaaatat
     5881 ttatagtatt caataccaac ttcaccccac tccctgtaag cttctgaatt tgaaggaata
     5941 gatggataca gcgtgctcca cggctctgca aacatggatg tgaacgatgg tatggcttga
     6001 tatggatgtc gaataaggaa aataattttt acatcgggaa atatgtcatg caacatctcc
     6061 agcctaccgg aactcatcac ggttttactc aaaaatatct tatcagagcc cagtgcatac
     6121 atccagcgct gtatagaagc cttgtagtat ttttttatgt tgtttttctc tttttcagat
     6181 aaattgtcag ggatatacaa ttcgttgaaa gatttgagat aaggcgtaat taatcctata
     6241 ctggggctga tacctgaaat aaagtgccag ccttcatctt cttccgattt attgaacccg
     6301 gttttatgga tttcatccca acctttaaac atcgtgtcat taattttatc caaaagccag
     6361 tgtaagggtc caccaatttt tttatcgata cctgaaatga tttgtactgc cctgtaaaaa
     6421 ataatggaat gaaacgtggt atgatagagc aagttgtaaa caaacttttt gtcatcaagg
     6481 cacatgagcc gatgtaaaaa tgtagtaccg cttcgcggat tgcttattat gaaaacaggt
     6541 tgatgtattt cgacctggtg ataattggga aataataatt cgtccagcaa cctacatatt
     6601 gcattgaaag aggaaactat aaaaatgaca ataaaatagg ctattccgat taaaaaattg
     6661 cgtattgaaa aatgtttgac caggtgcagg tagagtaact tatgatgtgt cttaagccgg
     6721 gtgtaaaata tatctggatg aataaaattc atattgcact ttgactatgc aaaataaacg
     6781 aaatcagctg agataatggt ttattctaaa tgaagttaga attattttat tgtgcagctt
     6841 tgctcagcca gaatatagcc attgttgtca ataatggaaa tgatataatc accgggttct
     6901 tgtattacag agtgcgtaac tatttgagat tgtccttttt ttacaaaaat atccttgttg
     6961 gaaaatcgcc agaaatagta tttttctacg cgtcttgcta tgctaagttt ataaactgca
     7021 ttattttgta aaggtttatc gtgattgaat acaatacaaa tattatcttt cttggcatcc
     7081 caagtagttt gagcttcaac aggaataccc tttttaacgg ttttgcataa gtagacttct
     7141 gctccgtgca tgatgtttga tgatacaaac aagagagtaa caagtaaggt tttcatgttt
     7201 cttttgttca aaattgtaaa aaagttcata tcgtttagcg ttagtatgca atctgaatca
     7261 aataccgtgc aaatttcttc aaaatacaag taataaattc aagcgattat tcacgcatgt
     7321 gaatttagtt tatcttcaat gttttacgca tgaaatttgt acttaaacta agaaaattgt
     7381 ggataatttt tgagttttat tgatttagaa tagatttttt aggaacttgc gacacaatcg
     7441 ctgaaatgac ttttgcggtt tgaatttttg aaaagaaaac atgtacttat tttttgatac
     7501 agaaacaacc ggactaccca aagactggaa agctccgtta agcaaactcg acaattggcc
     7561 acgaatggtg caactcgcct atatgcttta tgacaaagag ggaaataaac tgcaagaagc
     7621 agatttaatc ataaaaccag aaggatacac aattcccaaa gaggctgcgg atgtgcatgg
     7681 tatcactacc gatatagcta atcaaaaagg tattccactc gaactggctt tagcttcatt
     7741 ttatgaagcc gctcaaacag cagaagtgct ggtggcacac aatatggctt ttgacgaaat
     7801 gatagtagga gcggagttgt tgcgcaaaaa taaacacaat attattccga taaaaaaacg
     7861 aatctgtacg atgcaaagta ccgtgaattt ttgtaaaata ccgagtaagt acgggtttaa
     7921 atggcctaaa cttacagaat tgcattataa attgtttgac actgattttg tagacgcaca
     7981 taatgcctat gttgatattt ccataacggc taaatgtttt tggaagatga aagccatggg
     8041 aattatcgac tagatttata ccctacaatt aaacagaata tactggcctt atgatacatc
     8101 aaataataaa agataagtct actaagttgt ttattttttt aggcggcttt tttattgcta
     8161 atgcgtttat tgccgaagtg ataggcgtga aaatattttc attggaaaaa acattgggaa
     8221 ttagcccttt gcaaatcccg attttcggaa atgatttttc atttaatctt acagccgggg
     8281 ttttactttg gccggttgtt tttatcatga cggatataat caacgaatac tacggcgaac
     8341 gcggtgttcg tttcttatcc tatctcacag ccgggttgct cgcgtatgct ttcttcatgt
     8401 attatctcgc cattcaatta gtaccggcag actggtggcc tgcatctaaa gctgatgttg
     8461 gtgtaaataa tatgcaatcc gcctatgcac agattttcgg tcaaggattg gctattattt
     8521 tgggctcgct cattgctttt ttaatagggc aggttgtcga tgttgtcatt tttcataaaa
     8581 taaaaaaatg gagtggcgag aaatatatct ggctgagaag tacaggttct acggtagttt
     8641 ctcagtttat tgacagtttt gtcgtgttgt ttatcgcatt ttatttatat cccaaataca
     8701 tcagtgagtc caatggaaaa gcatggccat tttatgtagt gatgtcggta tgtgtaggca
     8761 attatattta taaatttttc atggctgtat tactaactcc tgttatctat tgggtacata
     8821 atggcattga caaatatttg ggtaagaact tagccacaga aatgaaacaa gctgccatgg
     8881 cagatgaaat acagtaatta ggtgcttgtc tcgtcctgaa aaccaccccc tttaattccc
     8941 cctccttttt caaggagggg ggggggaggt agaatcctga ttgcatcttg aaatgaaaac
     9001 gcctgccgga taaatccgtc aggcgtctcc taaaaataaa caccagtcta gtctaacgga
     9061 aataactctc cgttatgcat tttaaacaga tagtgctcac cactgatttt agcatctatc
     9121 aagtcgtcca gtttatcatt aagttcatct tgtttatcat taagctgatc ttgtacttct
     9181 tgtatggctt cctgcaaatc cggattattg gagtcgatat gaatatttat attggtagag
     9241 tctttcaggg tattcagctg gatttttaaa ttgcttattt cattagtatg atttcctgca
     9301 ttactccagt tgatatccaa tttatcttca tccataaaat cttcaacacg ctcgttccat
     9361 tttatttttt tgccttcagg tacataaatt tttacgatca gtttttgatt tctgaatttt
     9421 gattttggaa tatcgatacc gatgtaagga tcaataatta attgattacc tttttgtata
     9481 atattgtgcg tgatgtcagt agcattttcc aaagctgttt tttcatcctt accatttgct
     9541 gaaaattcac gtaccacgat aaatgaatct gaatttgtgg gtaatatttt tatgtcaaca
     9601 ggtatgttta tttcatcttt gcgagcgata aagttcatga aatcttgaaa actgtttatt
     9661 tgtacgccgt caatattgtc tttcttttct gtgaacgcca tgcttaaatc caaagtatca
     9721 atttttgaag cttcgtaaag aggcacactt tgcgttattt tttggtgttc agctatcagc
     9781 tttttacctt tagaatacac gatattcaac aataatatac caaataaaaa taatccgatt
     9841 aaagagaata aaatgttttt tttcagtggt ttgtttgttt tactgaatat atgaacgata
     9901 gccaatacga ataaaattat tggaatgaca gtagctaata aacctccgat gccaatcagc
     9961 cagcttactg aattattatc aaaaaaatat tttgtaagta acggattgcc ggcaactaag
    10021 gcaattccaa gcccaaaaaa tgttgccaac aaactgatta tgattgccac tgctactatg
    10081 cccagcacga acacgccaag gtataataat gatttgaaaa acaatttaat aaagccacca
    10141 ataaattcta agatttggtt ccctgctcct tgtctttgca caggttcttc tcttactgtc
    10201 ttttcaatag aagacagatt tacattttca ccgcgcatag tcagtttttg tgagctggta
    10261 gtagcttcag gtatgattaa ccacaagagt ggatatacca gaaaccctaa tccagtaaat
    10321 aaggtgatta atacaaagcc aattcggaac caaataggat catcaatacc aaaatatttg
    10381 gctaaacccg aacaaacacc ggctactatt ttatcatccg gatcgcggta tacacgtttc
    10441 tttgtttcat agcctgcatt ttgatagggt gttttattcg gttcaatttc aatatcatcc
    10501 tcagtatatg aagtgtcaaa ctcttgtggt cttcccatca tgcctatcac ttcgtctgta
    10561 tctttcttag ttacgatata tccttttcct tttgctaaga ataattcggc aaaacgggat
    10621 tctatgtctt ttatgatttc gtcacggtct atttcatttt tataaaattg tcgcaatgat
    10681 tgtaagtagg ctgaaagaga ttcgtaggca tcatcattga tggcgaatat ttgccctgcc
    10741 agattaatat tatatgtctt gttcattttg gtgtattttt tatggttaaa tagttggatt
    10801 ttgattagat tttatatcag ttgttttgac tacggcatca gagagttcat tccatgtttc
    10861 tttgagttca tcataagccc tgtagccggt acctgtaagg gtgaagtact ttcgaggagg
    10921 tcccgaggtg gattctttcc attggtaggt gagcaatcct gcatttttca atcttgtaag
    10981 cagagggtaa acagtacctt ccactaccaa aatattggca gctttcagcg agtcgagtat
    11041 gtctgatgca taaacttcgc cccgcgagat aataccgaga atgcaaaatt caagtatgcc
    11101 tttgcgcatc tgaactttgg tgttctccgt attaaaagtt gatgatgatg ttgttgtcat
    11161 aatttattgt tgattgtgat tgtgattggt cattgttgat tggtcattgg tgattagtta
    11221 ttggttattg gtttttagtt gtttgtgatt ggtgaatagt aattggttgt tatattatct
    11281 tgattcttga ttcttgattc ttgactcttg attcttgact cttgattctt gactcttgat
    11341 tcttgactct tgactcttga
//
LOCUS       DAOQII010000057        11290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2912_length_11290_cov_5.397241, whole genome shotgun sequence.
ACCESSION   DAOQII010000057 DAOQII010000000
VERSION     DAOQII010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11290
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_11290_cov_5.397241"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLF48_04260"
     CDS             complement(<1..1254)
                     /locus_tag="PLF48_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNF48571.1"
                     /translation="MKKVFLSILLLGLIVNGIAQTPLFPIPGAQVIPGEKINGTLDDI
                     SDFATRTQIPVPMPDGTKLMTDIFLPILQDSFTYVDTFDITILPAPFPPLRIPINAVL
                     LHKGFQYLIYDSINGAPNPNPYQLPLIMERTPYNKRGSVECSAMALLGYAGANQDMRG
                     RYSSQGAYLPLYSDSWKKWPYHNYTHVLDITNPSDPRNGNNHEDGYNTVEFIKNNLVR
                     AFDFNHDGIADDTALVYNGSIGTFGASALGYNQLQAAAAHKIDPTQPGLKALFPIVGP
                     LEFYKSTGFHNGCLRDMLVTGWLRGQIVDTRDDLMAFDTGLNDDIHSSKDYNTVDKFE
                     AANKAIDHFVTVRYENYPCGYYPNSISRSDMDATHAPVNAAGESEKNGMYSRYGNIEV
                     PVFHVSGWWDIFVDGSIETHDLIRRN"
     gene            complement(1490..2155)
                     /gene="msrA"
                     /locus_tag="PLF48_04265"
     CDS             complement(1490..2155)
                     /gene="msrA"
                     /locus_tag="PLF48_04265"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNF48572.1"
                     /translation="MISFIRHITLLIVLFAMPACKTANGNKSTLQNTETKTKKMDTTN
                     STSLATFGGGCFWCMEAVFQQMSGVEKIESGYSGGNTKNPTYKEVCTGETGHAEVIQI
                     TFNSSLVSFAELLKVFFTMHDPTTLNRQGNDVGTQYRSVIFYHNAEQQHEAEAVIQEI
                     NKQHIYPNPVVTQVVPFSVFYKAEDYHQNYYNQNKEQSYCRFVIQPKVEKFEKIFNDR
                     MKK"
     gene            complement(2317..2790)
                     /locus_tag="PLF48_04270"
     CDS             complement(2317..2790)
                     /locus_tag="PLF48_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48573.1"
                     /translation="MDENVDAKCTKIEIILYQNNRTVISYNADMSLALNADGVTLAER
                     ILTQIFACKNEKKHLEVGQEFCKVGIATLNFVSENFTLRTANLEQEAYFHFNKGFLKE
                     KVITNSKRNKPFTIIDFTPDKEIFGELNIQKEELENKIKRLKKDFINLSISTKYL"
     gene            complement(2933..5587)
                     /locus_tag="PLF48_04275"
     CDS             complement(2933..5587)
                     /locus_tag="PLF48_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF48574.1"
                     /translation="MFWDNNKKQENKEMTKYKFKELLIYSSDEWMARSAKKYRTVFDK
                     AETTYIRCELAFYNKLFDEEDWQCKVTLKAFDITGGKREQLCSLDKEVPVLKDENIVQ
                     VRDGWGNKELGVYWMKGDYVWEAYLDDVLIGSKNFFVNNVGLVTPHHNPYFVVDHISI
                     YNADADGWKLEPNQRVYYKEISKNDTKYLWAEVKIKNTTNLDWNYELFLNYYDDAGQH
                     KAIIPRVGKIEAGKLDWSYTFDIGWGTDDGGSWKDDKYSIELVFMDCLVAATTFNCGD
                     AFVEGQLELIDPATVSTLIVDANTEDKEDERTLEDLLAELDKLVGLQNVKQHIHDQIT
                     LINFNKLRKEQGLEDENKFSLHSVAIGNPGTGKTTVMRLLGKIYHKLGMLSRGHVHEV
                     DRVDLVGEYIGQTAPKVQKCIDEARGGILFIDEAYALSRAGEDSKDYGKEVIEILIKE
                     MSDGPGDIAIFVAGYPKEMRTFLESNPGLKSRFSNYFTFEDYLPDELQEIAYIIAERN
                     KLTIAEEAKLTLKDGLTEAFRSRDKSFGNARYVEGAINSAKKNMALRLMQLPDQKSLD
                     KETISTITQTDIAKVFQENKRKQLKLEVDKEDLFLALDELNTLVGLQNVKQEIDELIR
                     LIKYYNDIGKDVLNKFSLHSVFLGNPGTGKTTVARIFGKIFKALGILERGHLVEVDKE
                     ALVAGYVGQTAIKTNAKIEEAIGGVLFIDEAYSLTNSGNNGFGNEAVETLLKRMEDLR
                     GQFIVIAAGYTDNMNDFLQANPGLNSRFDKKYVFEDYNPEEMMQIADTFLKPEDLVLN
                     AEANEHLLHYFTALYEHRDKFFGNGRTVRQTILEAIKNQHLRMSNIPAAQRNMQDLQT
                     ITIDDVNEFKYNDDLRINKPIGFRLNQS"
     gene            5666..6076
                     /locus_tag="PLF48_04280"
     CDS             5666..6076
                     /locus_tag="PLF48_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF48575.1"
                     /translation="MEIEINRVNSKVHLAATNEQGIVVQMDGSPDIGGENLGARPMQL
                     ILMGLGGCTSMDVLSMLEKMREQVKAYKVIVRAERATEHPMVFTKIHVHYIFEGNLKK
                     ENVERAINLSMEKYCSVTHMLNKTATITHDYEIV"
     gene            6503..7060
                     /locus_tag="PLF48_04285"
     CDS             6503..7060
                     /locus_tag="PLF48_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF48576.1"
                     /translation="MTAPKKDILEVQNAIKVYDQLHDFNPFHLKDTEKAHSILMNGLV
                     ENAGKLRTTNVGIVKGSKVEHIAANGAMVRGLMKDLFEYLKKDKDLLLIKSCVFHYEF
                     EFIHPFIDGNGRIGRLWQTLLLMQQYPVFEFLPIEHLIKKRQKEYYHALSLSDKKGNS
                     TPFIAFMLDVISDVLDELLQSQNKT"
     gene            complement(7397..8353)
                     /locus_tag="PLF48_04290"
     CDS             complement(7397..8353)
                     /locus_tag="PLF48_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNF48577.1"
                     /translation="MKHKITNCLYCYQQLNETEQDFHSSCAKKMFGTKQAPIIDFNLK
                     QLEELAKQIVIKSVAVTGVQPKLSLELEKHKNELSRLTIVGLHGNFILKPPATEYKEL
                     PQNEDVTMHLANLVKIKTAQHSLIRLHSGELAYITKRFDRNKKDKIAVEDFCQLTENL
                     TEHKYRSSIEKVAKSTQKFTTNKGLETLRLFELVLFCYLTGNADMHLKNFALLENALG
                     EFELSPAYDLLSTVLVITDDKEETALTINGKKSRLKKKDFDALAISMNINTKVLESIY
                     TKFEKVLPNWIDFIKQSFLSKAMQKSYIELIQKKHYNLFQIS"
     gene            complement(8343..8669)
                     /locus_tag="PLF48_04295"
     CDS             complement(8343..8669)
                     /locus_tag="PLF48_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNF48578.1"
                     /translation="MRKGNVYYKNNLAGIIAENEDGYTFQYNEQYLNSTSAKSISLTL
                     PIQTQPYQSKILFPFFDGLIPEGWLLNIAVANWKLNARDRFGLLLTLCNDCIGCVSIK
                     ALENEA"
     gene            complement(8666..8872)
                     /locus_tag="PLF48_04300"
     CDS             complement(8666..8872)
                     /locus_tag="PLF48_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF48579.1"
                     /translation="MSTQLAEFVKEKRKQLKLTQPELAEKAGVGLRFLRELENGKESV
                     RLDKVNQVLALFGAKVGVVTNDEL"
     gene            complement(9104..10147)
                     /locus_tag="PLF48_04305"
     CDS             complement(9104..10147)
                     /locus_tag="PLF48_04305"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNF48580.1"
                     /translation="MKIRFVEADIKTICLHLVGNKLADENCVFSKKEIVIDSELNTLL
                     SSYFLSSFKSEEYYKFYHETNLSLNEVYTYVTDAFNEPNSIQKQSVNLAKHLYNQSTH
                     PNIKVGEFYVVHFQNVTLESKSVEAIGIFKSENKETFLEVEQITNSFEIENRKGININ
                     KLDKGCLIFNTNSEEGYIVSIVDNTNKGSEAQYWKDHFLGVQVLNNEFQQTSQFLGIT
                     KQFVTKQFTEDFEVSKTNQIELLNRSVDYFKKHDNFDRKEFEEEVLGDTNVIKSFRKF
                     DNEYRTENEIEIEDNFEISNQAVKKQARVFKNVLKLDKNFHIYIHGNSDLIEHGIDEN
                     GRKFYKIYYKEES"
     gene            complement(10144..>11290)
                     /locus_tag="PLF48_04310"
     CDS             complement(10144..>11290)
                     /locus_tag="PLF48_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48581.1"
                     /translation="QAFFRMNTEHKDIQKILPYGEQLRGFANQKYITEPELCNILKER
                     GIFTLSKDRDYTVPLLQTLMLSPKEFDKIREAFSTREDNPKKSSSDIIWAKDTKILIP
                     EILNVDVTDFIIKHLPTCTLEQAIRFSTVENNPNHLKTDFTINRNDINKVWYEQSNKF
                     DGTIEFINDINGKGRVVISHTAPETKELAEHIIKKQIAEYKKRGVIAPKEQLRKIFFR
                     DFTNEERFTFFFRLTNHLESEYFKHEDIKDIYIKPENTELPEEIKWMEELQKILLSGK
                     SLDKKVFIKDKKFHKHLLLWSIDASFEYSYKGEKGKFTANFGFPDFVTKNGSAEFELL
                     ISTITPLKTIDVRTKKILKFKLLSEMDKQKSIVYNNFLEYKNKVSLE"
BASE COUNT         3428 a         2026 c         1841 g         3995 t
ORIGIN      
        1 attcctgcgg attaaatcat gggtttctat tgagccatct acaaatatat cccaccagcc
       61 cgacacatga aatacaggca cttcaatatt tccgtagcgg gagtacatcc cgtttttttc
      121 actttcgcct gctgcattta ccggggcatg tgtggcatcc atgtcgctac ggctgataga
      181 attggggtag taaccacacg gataattttc gtagcgaacg gttacaaagt ggtcaatggc
      241 tttattggcc gcttcaaatt tatcaacggt gttatagtct tttgaagagt gaatatcatc
      301 attaagtccg gtatcaaatg ccattaaatc atctcttgtg tctacaattt gacctctcaa
      361 ccatccggtt accaacatat ctcgcaagca tccattatgg aagcctgtac ttttataaaa
      421 ctctaaaggc cccacgatag ggaacaatgc ttttagtccc ggttgtgtcg ggtcgatttt
      481 gtgtgcagcc gctgcctgca actgattgta gcccagtgcg gatgcgccaa atgtaccgat
      541 agagccatta tacactaaag cggtatcatc tgcaattcca tcgtggttga aatcgaaagc
      601 acgcaccaga ttgtttttga taaattctac cgtattataa ccatcttcat gattattgcc
      661 gttgcgagga tcactcgggt tggtaatatc caatacatgt gtataattat ggtaaggcca
      721 ttttttccag ctatcagaat acaatggtaa ataagcacct tgtgaagaat atcgtccgcg
      781 catatcctga ttggctcctg cataacctaa caaagccata gcagaacatt ctacactgcc
      841 tcttttgttg tagggcgttc tttccataat caggggcaac tgatacggat ttggatttgg
      901 agctccgttg atactgtcat agattaaata ttgaaatcct ttatgcaaaa gaaccgcatt
      961 tatgggaatg cgcaatggcg gaaaaggagc aggcagtatg gtgatgtcaa aagtatcgac
     1021 ataggtaaaa ctgtcttgca aaataggcag gaaaatgtct gtcatcaatt tggtgccatc
     1081 gggcatgggc accggaattt gcgtgcgtgt ggcaaaatct gaaatatcat cgagcgttcc
     1141 atttatcttt tctccgggaa ttacttgtgc gccgggaatg ggaaataaag gggtttgtgc
     1201 aatgccatta acaattaaac ccaaaagtaa aattgacaga aatactttct tcatagaact
     1261 tgttttctct aaaataagaa aaattaataa agtatgaaat gattttgcaa aaaaaatgaa
     1321 atgctgtttc tatgtcgtta agttaagtgt gatttaatgt tgaacatccc aaaatagttg
     1381 atttagtttt ttagaaccat ctccttttat tcctcctcca tgatgaaggt ggaggaacta
     1441 agattaaatc agctattttt agagttgacc aacttaacga tattgaatgc tattttttca
     1501 ttctgtcgtt aaatattttt tcaaatttct cgactttggg ctgaattaca aaacgacagt
     1561 aggattgctc tttattttgg ttgtaataat tttgatgata atcttctgct ttataaaata
     1621 cagaaaatgg cactacttgt gtcaccacag gatttggata aatatgctgc ttattaattt
     1681 cttgaatgac agcttccgct tcgtgctgtt gctccgcgtt gtgataaaat atgactgagc
     1741 gatattgcgt accaacatca ttaccttgtc tgtttaatgt agttgggtcg tgcatagtaa
     1801 aaaagacttt cagtaactct gcaaaagaaa ccaagctgga gttgaaggtt atttgaatta
     1861 cttctgcgtg tccggtttcg cccgtgcaga cttctttata ggtcggattt tttgtgttgc
     1921 ctccgctata acccgattct atcttttcaa cgccactcat ttgttgaaac acggcttcca
     1981 tgcaccaaaa acaaccaccg ccaaacgtgg ccaaagatgt cgagtttgta gtatccattt
     2041 tttttgtttt agtttccgtg ttttgcaggg tagatttatt tccgtttgcc gttttgcatg
     2101 caggcattgc aaacaaaact atcaggagtg taatgtgtct gataaaagaa atcatctctt
     2161 caaatgtaac aagaaatgtg cagagctgga attggatgca attttaaatt aaataaaacg
     2221 atttaatttt cgttaaatta aacccattga atgcggtata aaaatgaaaa caactattaa
     2281 tcctgatgtt ttatttgatt cgtaataaaa ttattcttaa agatactttg tacttattga
     2341 caaatttata aaatcctttt tcagcctttt tatcttgttt tccaattcct ctttttgaat
     2401 gttcaactca ccaaaaattt ctttatcggg ggtaaaatca attattgtaa aaggcttatt
     2461 tcttttacta tttgtaatta ctttttcttt taaaaatcct ttattaaaat ggaaatacgc
     2521 ttcttgctct aaattagctg tacgtaatgt aaaattttcg gaaacaaaat ttagcgtagc
     2581 aatgccaact ttacaaaact cctgtccgac ttccaaatgt ttcttctcat tcttacaagc
     2641 aaatatttgt gtaagtattc gttctgccaa ggttactcca tcagcattaa gtgccaagct
     2701 catatctgca ttgtacgaaa ttactgttct gttattttga taaagaatga tttcaatttt
     2761 tgtacatttt gcatccacat tttcatccaa ggcatgacat agtgcttcta caggaagaga
     2821 gcttaatgaa ttggaattaa aacaatcctc aaaatacaat ttaggccgga agcgaacagc
     2881 ctctgcagca ttaagagttg aaatggaatc tgcaggtatc atattcttct ttctagcttt
     2941 gatttaatcg aaaaccaatc ggtttgttta ttcttaaatc gtcattatac ttaaattcat
     3001 ttacatcatc aatagttatt gtctgtaaat cttgcatatt gcgctgtgct gcaggtatgt
     3061 ttgacatccg caagtgttga ttttttatcg cttccagtat ggtctgcctt acggttctgc
     3121 cattgccaaa aaatttgtcg cggtgttcgt agagtgctgt aaagtaatgt aagagatgtt
     3181 catttgcctc tgcattcaat accaaatctt caggtttcaa aaatgtatcc gctatttgca
     3241 tcatctcttc cggattataa tcttcgaaga catatttttt gtcaaatctg gaatttaatc
     3301 caggattggc ttgcaagaaa tcattcatat tgtctgtata tcctgctgct attacgataa
     3361 actgtccgcg caaatcttcc attcttttta gcaaagtttc cacggcttca ttgccaaaac
     3421 cattattacc ggagttagta agtgaatacg cttcatcgat aaacagcacc cctccgatag
     3481 cttcttcgat ttttgcattc gtttttattg cagtctgtcc cacatagccc gccaccaagg
     3541 cttctttatc tacctctacc agatgtccgc gttctaagat gccgagtgcc ttaaatattt
     3601 ttccgaatat acgggcaact gttgttttgc ccgtaccggg attgcccaaa aatacactgt
     3661 gcaaagaaaa tttatttaat acatctttgc cgatgtcgtt ataatatttt atcagcctta
     3721 tcagttcgtc aatttcttgt tttacatttt gcaaacctac cagtgtgttg agttcatcca
     3781 gagccagaaa taaatcttct ttgtctacct ccagcttcag ttgtttccgt ttattttcct
     3841 gaaatacttt ggcaatatct gtttgcgtaa tggtcgaaat cgtttctttg tctaaggatt
     3901 tctgatcggg caattgcata agccgcaagg ccatattttt tttagcactg ttaattgcgc
     3961 cttctacata acgagcatta ccaaaacttt tatcccggct gcggaatgcc tctgtaagtc
     4021 catctttcaa agtcagtttg gcttcttccg caatggtgag tttatttctt tcagcaatga
     4081 tataagcaat ttcctgaagc tcatcaggta agtaatcttc aaaagtaaaa taattgctga
     4141 agcgggattt taagcccgga ttcgactcta aaaaagtgcg catttctttc ggatatccag
     4201 ctacaaaaat ggcaatatca cccggtccgt cgctcatttc cttaatcaga atttctatca
     4261 cttctttgcc ataatcctta ctgtcttcgc cagccctcga caaggcatac gcttcatcaa
     4321 taaacaaaat accgcctcga gcttcatcaa tgcatttttg tactttaggt gctgtttgcc
     4381 ctatgtattc gcctaccaga tcaacacggt ctacttcgtg tacatgcccc cgggaaagca
     4441 ttcccaactt atgatatatc ttgcctaaaa ggcgcataac cgtagttttg cccgtgcccg
     4501 gattgccaat agcgactgaa tgtagggaaa atttattttc atcttccagt ccttgttctt
     4561 ttctaagttt attaaaatta atcagcgtaa tctgatcgtg aatatgttgt tttacatttt
     4621 gaagacccac aagtttatcc agttcagcaa gcaaatcttc cagtgtacgt tcatcttcct
     4681 tatcctctgt atttgcatca acaataagtg tagagacagt tgcaggatct attaattcga
     4741 gttgtccttc cacaaatgca tcaccacagt taaaggtagt tgcggctacc agacaatcca
     4801 taaaaacaag ctcgatgctg tatttgtcat ctttccatga gcctccatca tcagtacccc
     4861 aacctatgtc aaatgtgtaa gaccagtcga gtttgcctgc ttcaatcttg ccgacacgcg
     4921 gaatgattgc tttatgctgt ccggcatcat cataataatt taagaaaagc tcataattcc
     4981 agtcgagatt agtggtgttc ttaattttca cttccgccca caaatatttg gtgtcattct
     5041 tcgatatttc tttgtagtac accctttgat tgggctccag tttccagccg tctgcatcgg
     5101 cattatatat tgaaatatgg tcaaccacaa agtagggatt atgatgaggg gtaaccaagc
     5161 ccacattgtt tacaaaaaag ttctttgacc ctatcaacac atcatctaaa taggcttccc
     5221 atacataatc acctttcatc cagtatacac ccaactcttt attgccccag ccgtcacgta
     5281 cttgtacgat attttcgtct tttaaaaccg gcacttcttt atccagactg cacaattgtt
     5341 cgcgcttacc tccggtaatg tcgaatgctt ttaatgtcac tttacactgc caatcttctt
     5401 cgtcaaacaa tttattgtag aatgccagtt cacaacggat ataggttgtt tccgccttat
     5461 caaaaacggt tctgtatttt tttgccgaac gcgccatcca ttcatctgat gaataaatca
     5521 gtaactcttt aaatttgtac tttgtcattt ctttgttttc ctgtttttta ttgttatccc
     5581 aaaacatcct ttaaacttta aagtaaatat acctaaagca agattaatgt atgcgggaaa
     5641 gttaactatt ttctatattt agcacatgga aattgaaata aatcgggtaa acagcaaagt
     5701 tcatttagcg gcaacaaacg aacaaggcat tgtagtacaa atggatggtt caccggatat
     5761 aggcggtgaa aatctggggg caagacctat gcagttaata ttgatgggtt tgggtggttg
     5821 cacgagtatg gatgttctgt ctatgctgga gaaaatgcgt gaacaggtaa aggcttacaa
     5881 ggtaattgtt cgagcagagc gggcaactga acacccgatg gtatttacca aaatccatgt
     5941 acattatatt tttgaaggca atttgaaaaa agaaaatgta gagcgagcca tcaatttatc
     6001 tatggaaaaa tactgcagtg ttacgcatat gctgaataaa accgcaacta taactcatga
     6061 ttatgaaatt gtgtgagatg atacggcatg attcgaaatg agaagattat tctcaggtag
     6121 aaaataaaaa caagacggtt aaatcataat atgtaaacta tcaagtgaaa taaaatgcaa
     6181 aagcttagat ttctatagga agaaaaggca attttcaact tattattgag ttgcctgtct
     6241 agcgttgaat ttgtaatttt gatactatca aatgatacta tcataaatga aatcacatta
     6301 tacgattacc gatactattt tacatttaac acttgaacat atgtatttca gaaaagacag
     6361 gggctattaa tgctatgcat ttatataaac ctgcaaccga actcagaaat aagaatagaa
     6421 ttaagacgat tcaatcttct ttataaattg agggtaacac gcttacagaa gaacaaatca
     6481 ccgctttgtt agaaacaaaa gagtgacagc acctaaaaaa gatattttgg aagtgcagaa
     6541 cgctattaaa gtatatgatc agcttcatga ctttaatcct tttcatttaa aagatacaga
     6601 gaaagcacac agcatcttaa tgaatggctt agttgaaaat gcgggtaaat taagaacaac
     6661 aaatgtaggt attgtaaaag gctcaaaagt ggaacatatt gcagcgaatg gagctatggt
     6721 aagaggctta atgaaagatt tgtttgagta cctgaaaaaa gataaagact tgctccttat
     6781 aaaaagttgt gtgtttcatt atgagtttga atttatacat ccttttattg atggaaatgg
     6841 cagaataggt aggctctggc agacattact cttaatgcaa caatatcctg tatttgagtt
     6901 cctgccaata gagcatttaa taaaaaagag acagaaagaa tattatcatg cgctttctct
     6961 ttctgataaa aaaggtaatt caactccgtt catagcgttt atgttagacg ttatttcaga
     7021 cgtcttagac gaactcttac aatctcaaaa caaaacataa ctgcagctga tagagatgct
     7081 ttatttaaag agaaaacagg ctcaaaaatt tttagcagaa aagattatct caatgctttt
     7141 aaaaatattt ctgcccctac tgccagtcgt gatttggctt gggcagttga gcatggttta
     7201 ttggaaaaag agggtgataa gaggatggcg gtgtatgggt ttaaatagcc atttattttc
     7261 cgatacagaa agtaacctgc tcaatgtttt caatattttc agcatattgg gggacaaata
     7321 tcggacacaa tgtgtgaaaa catacacttt taaaggcagg aaaaggcaaa ttcaattacg
     7381 aattaaaaat tatcaattac gaaatttgaa acagattgta gtgttttttt tgtattaatt
     7441 caatataact tttttgcatt gcttttgata aaaaactttg cttgataaag tcaatccagt
     7501 tgggtaatac tttttcaaac tttgtatata tactttctaa aacctttgtg tttatattca
     7561 tgcttattgc taaagcatca aagtcctttt tctttaatct gcttttcttt ccattaatgg
     7621 ttagcgcggt ttcttctttg tcgtctgtaa ttactagtac tgtacttaat aaatcataag
     7681 caggagataa ttcaaattcg cctaaagcat tttctaataa tgcaaagttt tttaagtgca
     7741 tatctgcatt gccagttaag taacaaaaca ataccaactc aaatagccgc aatgtttcta
     7801 aacctttatt tgttgtgaat ttctgtgttg attttgcaac tttttctatg gaacttctgt
     7861 atttatgctc ggttaaattt tcagttaatt gacaaaaatc ttctactgca attttatctt
     7921 ttttgtttct atcaaagcgt ttagtaatat atgctagttc gcctgaatgc aaacgaatta
     7981 aagaatgctg tgcagttttt atttttacta aattggcaag gtgcatggtt acatcttcat
     8041 tttgtggcaa ttctttgtat tctgtcgctg gtggctttaa aataaaattg ccgtgtaacc
     8101 ctacaatcgt taatctcgat aattcgtttt tgtgtttttc taattctaat gataatttag
     8161 gttgaacacc agtaacagca acacttttaa taactatttg tttggctaat tcttctaatt
     8221 gttttaaatt aaaatcaatg attggtgctt gttttgtacc aaacattttt ttagcacaag
     8281 acgaatgaaa gtcttgttca gtttcattta attgttgata gcaataaagg cagtttgtta
     8341 ttttatgctt cattttctaa tgcttttatt gatacacaac cgatacaatc attacacaag
     8401 gttagcaata aaccaaatct atctcttgca ttgagtttcc agttagcaac tgcaatattc
     8461 aataaccatc cttcaggaat taaaccatca aaaaaaggaa ataaaatttt gctttgataa
     8521 ggttgtgttt gaatgggcaa cgttaggcta atagattttg cagaagtgga atttaaatat
     8581 tgctcattgt attgaaatgt atagccatct tcattttcag ctataattcc tgctaaatta
     8641 tttttgtaat aaacgtttcc ttttctcata attcatcatt tgttactaca ccaacttttg
     8701 caccaaataa ggctaaaact tgatttactt tatctaatct tacagattct tttccattct
     8761 ccaattcacg caaaaaacgc aaacctacac cagctttttc agctaattca ggctgtgtaa
     8821 gttttaactg cttacgcttt tctttcacaa attcggctaa ttgagtactc atttttatac
     8881 ctttaagggt acaaatttaa taagattaat tgaaattata cctttaaggg tataaatatt
     8941 gtgaatgata tttgaattat acccgaaggg gattgttttc cgatacaaaa agcatagtat
     9001 ttagtctaag tttgtgtata tcagttgatt atattgttgt gtgttatgaa ataatgtaaa
     9061 aatgcaataa atattgagaa agcaatgggg tattaaagga aaatcagctt tcctctttat
     9121 aatatatttt ataaaatttt cttccattct catctattcc atgttctatc aaatcactat
     9181 ttccgtgtat atagatatgg aaattcttat ccaatttcaa tacattctta aacactcgtg
     9241 cttgtttttt tacggcttga tttgaaattt caaaattatc ctcaatttca atttcatttt
     9301 cagttctata ctcgttatca aattttcgga aagacttaat tacattggta tctccaagta
     9361 cttcttcttc aaattctttt ctatcaaaat tatcgtgttt tttaaagtaa tctacagaac
     9421 gatttaaaag ttcaatttgg ttagttttag acacttcaaa atcttcggta aactgtttgg
     9481 ttacaaactg ctttgtaata cctaaaaatt gactggtttg ctgaaattca ttgtttaata
     9541 cctgtacacc taaaaaatgg tctttccagt attgtgcttc actcccttta ttggtattat
     9601 ctactattga aacaatgtaa ccctcttcac tattggtatt aaaaattaaa catcctttgt
     9661 ctaatttatt gatgtttatt ccttttcggt tctcaatttc aaagctattc gtaatttgct
     9721 ctacttctag aaaagtctct ttattttcag acttaaatat tccaatagcc tctactgatt
     9781 tgctttctaa agtaacattt tgaaagtgaa caacataaaa ttctccaact tttatattag
     9841 ggtgtgtact ttgattataa agatgtttag caaggtttac agattgtttt tgtatgctat
     9901 taggttcatt aaaagcatct gtaacataag tatatacttc gtttaatgac aagttagttt
     9961 cgtggtaaaa tttataatat tcctccgatt taaaagaaga taaaaaatag cttgaaagca
    10021 aagtatttaa ctcactatca attactattt ccttttttga gaatacacaa ttttcatctg
    10081 caagtttatt tccaactaaa tgcagacaaa tggttttaat atcagcttct acaaacctta
    10141 ttttcattcc aaacttactt tatttttata ctccaaaaaa ttattataca caatagattt
    10201 ctgtttatcc atctctgaca ataatttaaa ctttaaaatc ttttttgttc ttacatcaat
    10261 agtttttaat ggagttatag tagaaataag taattcaaac tccgcacttc catttttagt
    10321 tacaaaatcg gggaaaccaa aatttgcagt aaatttccct ttttctcctt tataactata
    10381 ctcaaatgag gcatcaatac tccacaatag taaatgttta tgaaattttt tgtccttaat
    10441 aaaaactttc ttgtctaaag atttacctga taaaagtatt ttttggagtt cttccatcca
    10501 ttttatttct tctggtagtt ctgtgttttc aggtttaata tatatatctt taatatcttc
    10561 gtgtttaaag tattcacttt ctaaatggtt tgttaatcta aagaaaaatg taaacctttc
    10621 ttcgttggta aagtctctaa aaaatatttt ccttaactgc tcttttgggg caattactcc
    10681 tcgtttttta tactctgcaa tttgtttttt aataatatgc tctgcaagtt ctttggtttc
    10741 aggtgcagta tgagaaatta caaccctgcc ttttccgtta atatcattta taaattcaat
    10801 agttccgtca aatttgttgc tttgctcata ccaaacttta tttatatcat ttctatttat
    10861 tgtaaaatcc gtttttaaat gatttggatt attttcaact gtagagaacc gaatggcttg
    10921 ttctaatgtg caagttggca agtgctttat aataaaatca gttacatcaa cattcaatat
    10981 ttcaggtatc aaaatttttg tgtcttttgc ccaaataata tcgcttgaac tctttttggg
    11041 gttatcttct ctcgtagaaa atgcttctct tattttgtca aattctttag gagaaagcat
    11101 taaggtttgt aataaaggaa ctgtataatc tctatcttta cttaaagtaa aaatgcctct
    11161 ctcttttaaa atattacaaa gttcaggttc ggtaatgtat ttttgattag caaagcctct
    11221 taattgctct ccataaggca atattttttg tatgtcttta tgttctgtat tcattctaaa
    11281 aaacgcttga
//
LOCUS       DAOQII010000058        11136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_2984_length_11136_cov_5.098006, whole genome shotgun sequence.
ACCESSION   DAOQII010000058 DAOQII010000000
VERSION     DAOQII010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2984_length_11136_cov_5.098006"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLF48_04315"
     CDS             complement(<1..1320)
                     /locus_tag="PLF48_04315"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNF48582.1"
                     /translation="METDCNTLKLSGLEPLIVTKSSNFINIGERTNVTGSKVFLKMIK
                     EERFEDALAVAMDQVRGGAQVLDINMDEGMIDGVKSMVNFLNLIASEPEIARIPIMLD
                     SSKWEIIEAGLKCVQGKSIVNSISLKGGEEEFVRQAKLIKKYGAATVVMAFDENGQAD
                     SYQRRIDICKRSYDILVNIVKFNSNDIIFDPNIFPVATGMDEHKNNALDFFNATRWIR
                     ENLPGSHVSGGVSNVSFSFRGNNIVREAMHSAFLYHGIKHGMDMGIVNPTMLEVYDDI
                     DKELLERVEDVLLNRRDDATERLLEFADTVKGTAKKKEEDLSWRNQPVNERLKHALVK
                     GLIEYIDEDTEECRQQYDLPLHVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKK
                     AVAYLQPYILASQSGEAKKLGKILLATVKGDVHDIGKNIVGVVLACNN"
     gene            complement(1358..1720)
                     /locus_tag="PLF48_04320"
     CDS             complement(1358..1720)
                     /locus_tag="PLF48_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF48583.1"
                     /translation="MAFKFEELKIWQKALEISSKVNELSKKFPKEEIYVLTSQIKRAA
                     DSISLNIAEGSTGQSDAEFNRFLGIAMRSAIEVIACLYIARKREMINDNQFNELYNEI
                     ESLIVSIQSLRKILKIKK"
     gene            complement(1733..2743)
                     /locus_tag="PLF48_04325"
     CDS             complement(1733..2743)
                     /locus_tag="PLF48_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNF48584.1"
                     /translation="MTNDLKQIAQQKILILDGAMGTMIQRYKLDEADYRGDRWKNHEH
                     PLKGNNDLLSITRPEIIKEIHRQYFQAGADIVETNTFSGTTIAQADYHLEDAVYDINF
                     QSAKIAKEVAQEFTDFPRFVAGAMGPTNRTASISPDVNNPGFRAITFDELVKAYYQQA
                     KALIDGGVDLLLIETIFDTLNAKAALFAVQSLFEDIGKEYPVSISGTITDASGRTLSG
                     QTTEAFLISMSHVNLFSIGLNCALGAKDLRPYLQVLAKEAPFLISAYPNAGLPNAFGG
                     YDETPEQMAEAVREYLDLGLVNILGGCCGTTPAHIKCFAEVSKNYKPRKLQTVFSPQT
                     TV"
     regulatory      complement(2926..3096)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3214..3807)
                     /locus_tag="PLF48_04330"
     CDS             complement(3214..3807)
                     /locus_tag="PLF48_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HNF48585.1"
                     /translation="MKKLTFLLLICIALLNGCKKETGPTTKTENPLNASLAGGQIDSI
                     RTYNVGAFEFGNKLYFSKNGSITKLGCKLPYKGNIRVSFWDYSTKDLIAAATITITDS
                     TKFNYVNISPIAVTANTRYVLSLNNTSGGLARSYHAGYRKSGATLVANIYPFTVGSVT
                     YEAPYYQGSSTSTFPINEDPTKNYIGGFSDLQFEYTE"
     gene            complement(4259..4846)
                     /locus_tag="PLF48_04335"
     CDS             complement(4259..4846)
                     /locus_tag="PLF48_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HNF48586.1"
                     /translation="MNKNMNFISIFFILFLVMTTQLSCKKDKSVTPTQTEVKPIQNTE
                     LRTTYYNRTLNNGYTTSYEHGITFSSNAKGKITKVGGKVPKAGNYRITVWNDSSKAVL
                     ATTYADLDSLSYKYVSITPISVPANKRFVVSINVLKDAFEIRDNSVYDFMPFYADGIT
                     IHYYSDAKTASQTFPDRFTYTYKIAGLVDFVFVAD"
     gene            4992..5948
                     /gene="metF"
                     /locus_tag="PLF48_04340"
     CDS             4992..5948
                     /gene="metF"
                     /locus_tag="PLF48_04340"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNF48587.1"
                     /translation="MKVTDILAQKKNESLVSFEILPPLKGKSIASITKILDPLMEYKP
                     PFIDVTYHREEFEYKQNPEGYFEKIAVRKRPGTVGICAAIKYRYNVEAVPHFLCGGFS
                     KEETENALIDLGFLGIHNILALRGDARKLEKKFVPEPDGHAFALDLVEQINKMNNGVY
                     LDADLINPVKTDFCIGVAGYPEKHFEAANYEQDLHYLKQKIDAGGDYIVTQMFFDNAV
                     YFKFVEDCRKMGINVPIIPGIKPITKMGQLNSIPSAFFINLPEALVKEVRKCKEDSEV
                     KEVGTEWCIAQSKELVKFGVPCLHYYTMGDVKTISTICKEVV"
     gene            complement(6028..6798)
                     /locus_tag="PLF48_04345"
     CDS             complement(6028..6798)
                     /locus_tag="PLF48_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNF48588.1"
                     /translation="MARKKELTTVADKLEHLEELQTMHSKIDAITVLKGELPIEVADL
                     EDEIVGMEKRIAKIEGEINDAKEEIEHRKTLMKEAQTLITKYDKQLHNVKNNREFDAL
                     NKEIELQKLEVQLSEKKIKEANFTIENFQTQLEEVTKRIKSKKKDLEAKKKELDNIIA
                     ETDKEEKELGEKAAEIASEVDERLLTAYNRIRKSYKNGLAVVKITRNACGGCFGSIPP
                     QMQAEIRTKKKIMTCEHCGRIFAGVDDSEITLSDEEVA"
     gene            complement(6801..7895)
                     /locus_tag="PLF48_04350"
     CDS             complement(6801..7895)
                     /locus_tag="PLF48_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNF48589.1"
                     /translation="MKIAEIIQTIEEFAPLSYQESYDNAGLIIGDRNAVCTGVLICLD
                     TIEAVIDEAICKKINLVVAHHPIVFSGLKKITGKNYIERVVLKAIKNDIAIYACHTNL
                     DNVILGVNHQIANQLGLVNRKILEPQKKLLKKLYTYVPFNKTDEVLEALFRAEAGNIG
                     NYNECSFQVSGSGSFKGNEKAMPFVGEKGKRHIESENKIEVIFPGYKENNILRALKEA
                     HPYEEVAYEIITLDNTFQEMGAGMIAELASPMEEIAFLSFLKDKMQTACIRHTALLNK
                     PVKRVAFCGGAGSFLLNQAIAQKADVFISGDFKYHQFFDADNQILICDIGHFESEQFT
                     SQLFNKILSEKFPTFAVLISEINTNPVNYF"
     gene            7909..8994
                     /gene="lpxK"
                     /locus_tag="PLF48_04355"
     CDS             7909..8994
                     /gene="lpxK"
                     /locus_tag="PLF48_04355"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNF48590.1"
                     /translation="MLNFISKILLFPFALLYGSIIWLRNLLYKRGFLGSTDFEIPVIS
                     VGNLAVGGTGKTPFVEMLIELLYPYYPAGVLSRGYNRKTSGYLNVQTNNTVLDVGDEP
                     LQIKLKYPFVPVSVGEQRVFAIPQMMHHFPFLKLLILDDAFQHQSVRPDINILLTDYH
                     KPFWNDQILPMGTLREFTSGKERANIIVVTKCPPDLGLQEQIDLRVKIQPSANQKVFF
                     TSIQYFIPYHLFRPDEKLNYNNLSSVILLTGIADANPLKGEISKHVQDIIHFDYADHH
                     QFSVEELEAIKKHYPDNKIWLTTEKDAVRLLLHKDWFLQNDIDLFCMHIKTRFIGENK
                     DEFKFLLLDFLSFFYKEEIQNVPEQMD"
     gene            9151..10059
                     /locus_tag="PLF48_04360"
     CDS             9151..10059
                     /locus_tag="PLF48_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF48591.1"
                     /translation="MEILLATYNGEQFLRLQLDSIFAQSYKTFRLIIRDDGSTDATLA
                     IIKEYQNLYPDKIYLFRDESKQVGAIQNFSLLLQNSEADYIFLCDQDDIWHENKMELS
                     LQKIKSMEAEHPQQPILVHHDMEVIDENNEQLHPSKWTLENNNPKHNKLQYLLGSNHV
                     QGCTVLINKALRQQLLPIPKEAVMHDHWLALLAVSIGKIGYLPEALGKYRIHAHNVTG
                     QTRNGKKRSFTSNKNINKLYSSLFLQASAFLQQYRQLLSDSATNLIQNFLNLQTAGFL
                     KRKYLIIANSLYRPTWPKTLFFILRF"
     gene            10107..11000
                     /locus_tag="PLF48_04365"
     CDS             10107..11000
                     /locus_tag="PLF48_04365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF48592.1"
                     /translation="MLTYNSAKHLPVFFQCMAKQTYKQYTLFILDNHSEDNSVAITEQ
                     QELPIVLIKNETNVGFAKGNNLAIAMALDADYEFILLLNNDIECDEFLFENMVQKLTA
                     SEEHQLLSPKILFYPQTEYIWYAGCLFDKYKGNNTIIRGLYEHDNGQYDTPETMDFAP
                     ACCLLFHRKVLETVGLFDEKYFAYYEDADLLFRVKTQSKYKLLYSPDIKMYHKVGGSF
                     NARQDLKNVNQFTFGNQYIHLTTRNFVYYLLKQKLFWSYCYLLVFFFRMQARFFFSGK
                     YKVNLKTYRLLLRSYFEGFVL"
BASE COUNT         3386 a         2063 c         1965 g         3722 t
ORIGIN      
        1 attattacaa gccagtacaa cgcctacaat atttttacca atatcgtgga catcgccctt
       61 tacagtggct aataatattt tacctaattt ttttgcttcc ccgctttgcg aagccaaaat
      121 atatggctgt aaataggcta cggctttttt cattaccctt gccgatttta ccacttgcgg
      181 taaaaacatt tttccactgc caaacaaatc accaactaca ttcatgccgt ccattagtgg
      241 accttcaata acatgcaaag gcaaatcgta ttgttgccgg cattcttccg tgtcttcatc
      301 aatatattca atcaagcctt ttactaatgc gtgttttagt cgttcattta cgggctgatt
      361 tctccaagat aaatcttctt cttttttctt ggctgtacct tttacggtat cggcaaattc
      421 taataatctc tctgtagcgt catctcttct gttgagcaaa acatcttcca ctcgctccaa
      481 taattctttg tcgatatcat catatacttc caacatcgta gggttcacga tgcccatatc
      541 cataccgtgt ttaatgccgt ggtacaaaaa tgccgagtgc atggcttcac gcacgatatt
      601 atttcctctg aatgaaaatg aaacattgga cactcctcct gaaacatgcg acccggggag
      661 attttctcgt atccacctgg tggcgttaaa gaaatccagc gcattgtttt tatgttcgtc
      721 catgcccgtt gctaccggaa aaatattggg atcgaagatg atatcgttcg agttgaattt
      781 tactatgttg actaaaatat cataactgcg cttgcagata tcaattctgc gctgatagga
      841 gtctgcctgt ccgttctcat caaatgccat gaccacagtg gccgctccat atttttttat
      901 caatttggcc tgacgaacaa attcttcttc accgcctttt aatgaaatgg aattgacgat
      961 agattttcct tgtacgcatt ttaatccggc ttcaataatt tcccatttgg aagaatcgag
     1021 cattatgggt atgcgggcaa tttctggttc agatgcaata aggtttaaga aattgaccat
     1081 tgattttacg ccgtcaatca tgccttcatc catgttgata tcgagtactt gcgcaccgcc
     1141 acgcacctga tccattgcca ctgctaaagc atcttcaaag cgttcttctt taatcatttt
     1201 tagaaagacc tttgagcctg ttacattcgt tcgctcgccg atatttataa aattagatga
     1261 tttcgttaca ataagaggct caagcccgct tagttttaga gtattacagt ctgtttccat
     1321 attttaatgc attcagtcca cagacgactg tcgactgtta ttttttaatt tttaaaattt
     1381 ttcttaatga ttgaattgaa acaattaatg attctatttc attataaagt tcgttaaatt
     1441 gattatcgtt tatcatttct ctttttcttg caatatataa acaagctata acttcaattg
     1501 cagatctcat tgcgattcct aaaaacctat taaattctgc atcactttga cccgtagaac
     1561 cctcagcaat atttaatgaa attgaatcag cagctctctt aatttgagaa gttaatacgt
     1621 atatttcctc ttttggaaat tttttggaaa gttcatttac cttagaagaa atttctaatg
     1681 ccttttgcca aatctttaat tcttcaaatt taaatgccat aatatataac aattatactg
     1741 tggtctgtgg actaaaaact gtttgcaatt ttcttggttt ataatttttt gatacttcgg
     1801 caaagcattt gatgtgggca ggtgtggtgc cacagcaacc gcctaatata ttgacaaggc
     1861 ccaggtctaa atattcgcgt acagcctctg ccatttgttc gggagtttca tcataaccgc
     1921 caaaagcatt gggtaagcca gcattgggat aggcactaat caaaaatgga gcttcttttg
     1981 ccagcacttg caaatatggt cgcaggtctt tagccccaag tgcacagttt aatccaatcg
     2041 aaaacaaatt aacatgcgac atcgaaatta aaaacgcttc tgtggtttgt ccgcttaatg
     2101 ttctgccgct agcatcggta atcgttcctg aaatagaaac aggatattct tttccgatat
     2161 cttcaaaaag cgattgcact gcaaatagag cagctttagc attgagtgta tcaaagatgg
     2221 tttctataag caataaatcg acgccaccgt ctattaatgc tttggcttgt tggtaataag
     2281 ctttaacaag ttcatcgaac gtaatggctc tgaaaccggg attattgaca tcgggtgaaa
     2341 tagaagcagt acgatttgtt ggacccatag cacctgctac aaatctcgga aaatcggtaa
     2401 attcctgtgc tacttctttg gcaatttttg ccgattgaaa attgatatcg taaacggcat
     2461 cctccagatg ataatcggct tgcgcaatgg tagtaccact aaacgtattg gtttctacaa
     2521 tatcagcacc tgcctgaaaa tactgacgat gaatttcttt aataatttca ggacgagtga
     2581 tagacaataa atcattattg cctttaagcg gatgctcatg gtttttccat ctgtcgccac
     2641 gatagtcggc ttcatcgagt ttgtaacgct gtatcatggt tcccatggct ccatcgagga
     2701 tgagaatttt ttgttgtgct atttgtttta agtcattagt cattagttat tagtttattt
     2761 gctttctgca atcggaccac tgaccacaga atgtgaagat atttttttat acaactacta
     2821 aaacaaaaag aatgcaggaa tatgaaagaa tataaatctg tagtctgtta gccatgagca
     2881 gttgactaat tacgaaaatg aagaaaaaag atggatgaag acatttactt attttccact
     2941 tgttagtgta ggatttggca ccatttgttg agatgagaag aatcaagagt caagaatcaa
     3001 gacaaatgtc tataaatctt ggttcttggt tctaataatc ctacacggtt gccaagacat
     3061 catcgggcct attccctccg tctttcgtaa taagtttaat aaataaagaa caatatgcaa
     3121 atgtacacaa aagaaataag aatgcaaatt gccatagggc aataaaaaaa gcccaaagga
     3181 ttttttgggc tttttacgag aatatatata atattattct gtatattcaa actgcaaatc
     3241 agaaaatcct ccaatataat ttttagtggg atcttcattg attggaaagg tagatgtgga
     3301 agatccctga taataaggag cttcatatgt gacgctgcca acagtaaatg gataaatatt
     3361 tgcaacaagg gttgcaccag attttctata acccgcatga tagcttcttg ccagcccgcc
     3421 agaggtgtta tttaatgata atacatatct ggtattagcg gttactgcaa ttggtgatat
     3481 gttaacataa ttgaatttag tagagtctgt aatagttatt gtagcagcag caattaaatc
     3541 ttttgtggaa taatcccaaa aagacactcg aatattgcct ttatatggta atttacatcc
     3601 cagcttggtt attgagccat tctttgaaaa atacaattta ttaccaaatt caaacgcacc
     3661 gacgttatat gttcttatgc tgtctatttg cccacctgca agagaagcgt ttaggggatt
     3721 ttctgttttt gtagttgggc ctgtttcttt tttgcatcca tttaaaaggg caatgcaaat
     3781 aagcaagaga aatgttagtt ttttcatatt ttttaatttt aatgtaaata tagtttaata
     3841 attttaattc agaaacaaat aatgagtatt cgggcggttt tattgagtat tcgtttgttg
     3901 aatgttttaa cttttaggta gcacgttaac agcccgacag aaatgccggg ctgttaagtt
     3961 agtatcttaa ctgttactag aatattaagg taaaaacaca ggcaccagtg tatttctatc
     4021 agttgtagtg ccatctccta attgtccaaa attatttaag ccgcaagacc aaagagtgtt
     4081 gttggtttta agcactaagc tgtgaattac acctgcactt atttttattt gcggttcttc
     4141 agtggtggtt ttatctttct tacaagagat ggctgttatt cgattaacaa gaataagact
     4201 gtaaatatga ttagactttt gattgtcttt tattttttaa ataagaaata tattgatttt
     4261 aatccgccac aaaaacaaaa tctactaaac cagctatttt gtaggtgtat gtaaatctgt
     4321 ctggaaatgt ctgcgatgct gttttcgcat cgctgtaata atgaatagta ataccatcgg
     4381 catagaatgg catgaaatca tacacgctat tatctctgat ctcaaaagca tcttttaaaa
     4441 catttatgct cactacaaat cttttattcg caggcactga aataggtgta atgcttacat
     4501 atttataact taaagaatct aaatcagcat aagtggtagc taaaacggct tttgaagaat
     4561 cattccatac ggtaattctg taatttcccg ctttcggaac cttgccacct actttagtta
     4621 ttttgccttt tgcattggat gaaaaggtaa ttccgtgttc ataagatgta gtgtagccat
     4681 tgtttaaagt ccggttataa tacgtcgttc tcaattcagt attttgtatc ggttttactt
     4741 ctgtttgagt tggagtcact gatttatctt tcttgcagga aagttgggtg gtcatcacca
     4801 aaaacaggat aaaaaagatg ctgataaaat tcatattctt gttcatgtgt taaaatttat
     4861 cttaaagata ggcagcttat ttcaaaattg agagcatatt gagtattcgg caaaattcag
     4921 tgagaattcg ttataaaagc ccatttttta ttcccttttc tatttattaa cttcgcagac
     4981 tattttacaa tatgaaagta acggacattt tagcacagaa gaaaaacgaa tcgctcgtat
     5041 cttttgagat attacctcca ttaaaaggaa aaagcatagc atccatcacc aaaatattag
     5101 acccattgat ggaatataaa cctccgttta tagatgtaac ttatcaccgt gaggaatttg
     5161 aatacaagca aaatccggaa ggatattttg aaaaaatagc cgtgcgtaaa cgaccgggta
     5221 cggtgggtat ttgcgcagcc ataaaatacc gctataatgt ggaggctgtt ccacattttt
     5281 tatgcggtgg ttttagcaaa gaagaaactg aaaatgcgct tatagattta ggatttttag
     5341 gcatacacaa tattctggct ctacgcggtg atgccagaaa gctggagaaa aaatttgtac
     5401 ccgaaccgga tggtcatgct tttgcactcg acttggtaga gcaaataaac aagatgaata
     5461 atggcgttta tctggatgct gatttaatca atccggtgaa aaccgacttt tgcattggtg
     5521 ttgccggcta tcccgaaaaa cattttgaag ccgccaacta cgaacaggat cttcattatc
     5581 taaaacaaaa aattgatgcc ggcggtgatt atattgttac acagatgttt tttgacaatg
     5641 ccgtttattt caaatttgta gaagactgcc gaaaaatggg cataaatgtt ccaattattc
     5701 cgggcattaa gcctataacc aagatgggac aactgaacag cattccgtct gcctttttta
     5761 tcaacctgcc agaagcttta gtgaaagaag tacgcaagtg caaagaggac agcgaagtaa
     5821 aagaagtggg taccgaatgg tgtatagcgc aaagcaaaga gctggtaaaa tttggtgttc
     5881 cttgtttaca ttattatact atgggcgatg taaaaaccat ctcaacgatt tgtaaagaag
     5941 tggtgtaata atttagtact tacactaatt tttgcataaa aaaaccgcca agtatatggc
     6001 ggttttttta tttctaaatg gcaattatta tgctacttct tcgtctgaaa gtgtgatttc
     6061 tgaatcatcc acaccggcaa agattctgcc acagtgttcg catgtcatta tttttttctt
     6121 agtacgtatt tctgcttgca tttgcggagg tattgaacca aaacatccgc cacaggcatt
     6181 acgggtaatt tttacaaccg ccagtccgtt tttatatgat tttctgatac ggttatacgc
     6241 cgtcagtaat ctttcatcaa cttcagaagc aatttcagcc gccttttctc ccaactcttt
     6301 ttcttcttta tccgtttctg caataatgtt atccagttct tttttcttcg cttccaaatc
     6361 tttctttttg cttttgatgc gcttggtaac ttcttcgagc tgtgtctgga agttttctat
     6421 cgtgaagtta gcttctttta ttttcttttc gctcaactgt acttctaact tttgtaattc
     6481 aatttcttta ttcaacgcat caaactcacg gttgtttttc acgttgtgca attgcttgtc
     6541 gtattttgta atgagcgttt gcgcttcttt catcagtgtt ttgcgatgtt caatttcctc
     6601 ttttgcatca tttatctcac cctcaatttt agcaattcgc ttttccatgc ccacgatttc
     6661 atcttccaaa tcggcaactt ctattggtaa ttcgcctttc aataccgtaa tggcatcaat
     6721 tttggagtgc atggtttgca gttcttctaa atgttccagt ttgtcggcaa cagtggtgag
     6781 ttctttttta cgagccatat ttaaaaatag tttaccggat ttgtgttaat ttctgaaatt
     6841 aggacggcaa aggtaggaaa tttttccgat aatattttat taaatagttg tgaagtaaac
     6901 tgctcacttt caaaatgtcc aatatcacat ataagtatct gattatcagc gtcaaaaaat
     6961 tgatggtact taaaatcacc cgaaataaac acgtcggcct tttgtgcaat agcctgattg
     7021 agtaagaaac tacccgctcc tccacaaaat gccacccttt tcacgggctt attcagcaat
     7081 gccgtatgcc gaatacaagc cgtttgcatt ttgtctttca aaaaggaaag gaaggcaatt
     7141 tcttccatcg gagatgctaa ttctgcaatc atacccgcac ccatttcctg aaaggtatta
     7201 tccagggtaa taatttcata agctacttct tcgtagggat gtgcctcttt taaagcccga
     7261 agtatgttgt tttctttata tcctggaaaa attacttcta tcttgttttc agattcaata
     7321 tgtctttttc ctttttcgcc aacaaatggc attgcttttt catttccctt aaaacttcca
     7381 gaaccactta cctgaaaact gcactcgttg tagttaccaa tattacccgc ttctgctcta
     7441 aataatgcct caagtacttc atcggtctta ttgaagggta catacgtata taatttcttc
     7501 agcagctttt tttgcggttc taaaattttc ctgtttacaa gcccaagctg atttgcaatt
     7561 tgatgattga ctcccaatat tacattatcc aaattagtat gacaggcgta aatggcaata
     7621 tcgtttttga tggctttaag cacaacacgt tctatatagt ttttaccagt tattttcttt
     7681 aatccagaaa aaacaatggg atgatgtgct accactaagt ttattttctt gcagattgcc
     7741 tcatcaatta cagcctcaat agtatccaga caaataagta cgccagtaca tactgcattt
     7801 ctgtcgccaa tgattaatcc ggcattatca tagctttcct gataggataa tggtgcaaat
     7861 tcctctatgg tttgaattat ttctgctatt ttcatggtgt tttttatcat gttaaatttc
     7921 atctcaaaaa tactattatt tcctttcgct ttgttatacg gcagcattat ctggctgaga
     7981 aacctattgt ataagcgtgg tttcttgggt tctacagatt ttgaaattcc tgttatcagt
     8041 gtgggcaatc tggctgtggg tggtaccgga aagacacctt ttgtagaaat gctgatagag
     8101 ctgctttatc cctattatcc tgccggcgtt ttgagcagag gatacaatcg caaaacttcg
     8161 ggctatttga atgtgcaaac gaacaataca gtgcttgatg ttggcgatga gcccttgcaa
     8221 ataaaactta aatatccctt tgtgcccgtc agtgtaggcg aacagcgtgt atttgccatc
     8281 ccgcaaatga tgcatcattt tcctttttta aagttgctga ttttagacga tgcctttcaa
     8341 catcaaagtg taagaccgga tataaatatt ttattgactg attatcacaa accattctgg
     8401 aatgaccaaa ttttgcccat gggcacttta cgtgagttta cttcaggaaa agaaagagca
     8461 aatattattg ttgtaaccaa atgtccgcct gatttaggcc tgcaagaaca aattgatttg
     8521 cgggtgaaaa tacagccttc ggctaatcag aaagtatttt ttaccagtat acagtatttc
     8581 attccttatc acttgtttcg cccggatgaa aaattgaatt acaacaattt gtcttcagtc
     8641 attttgctga cgggtatcgc tgatgccaac ccattaaaag gggaaataag caagcatgta
     8701 caggatatta tacattttga ttatgccgat catcatcagt tctctgtcga agaattggaa
     8761 gctattaaaa agcattatcc ggataataaa atttggctca ctacagagaa agatgctgtt
     8821 cgacttttgt tgcacaagga ctggtttttg caaaatgata ttgatctctt ttgtatgcac
     8881 atcaaaacca gattcattgg cgaaaacaaa gacgaattca aatttttatt gctcgatttt
     8941 ctttctttct tttataaaga ggagatacaa aatgtgcccg aacaaatgga ttaatttaat
     9001 ttcagataaa ctacacagat aaagaattat tatataatga ctgaatttag taaatttgga
     9061 actgcttaaa ttttagttca tttattttag aatatcagaa aatgggttta tccattaaaa
     9121 aacagaaata cagctgcaca aatggaaaca gtggaaatac ttttagcaac ttataacggc
     9181 gaacagtttt tgcgactgca gctggatagt atttttgcac agtcttacaa aactttcagg
     9241 ttaattatac gcgatgatgg ctcaactgat gctacactgg caattatcaa ggaataccaa
     9301 aacctttatc cggataaaat ttacttattt cgtgatgaaa gcaagcaagt tggagcaata
     9361 caaaatttct ctcttttgtt gcaaaacagt gaagctgact atattttttt atgcgatcaa
     9421 gatgatatct ggcacgaaaa taagatggaa ttatcacttc aaaaaataaa atcaatggaa
     9481 gccgaacatc cgcaacagcc cattttagtt caccacgaca tggaagttat tgatgaaaat
     9541 aatgaacagc tacatccttc caaatggacc ttggaaaata ataatccaaa acataataaa
     9601 ttacaatatt tactgggaag caaccatgta caaggctgta cagtactcat caataaagca
     9661 ctacggcaac agcttttacc cattccgaaa gaggctgtca tgcatgatca ttggttggct
     9721 ttactggcag ttagcatcgg aaaaataggg tatttgccag aagcgctcgg caaatacaga
     9781 attcatgcac ataacgtaac cggacaaacc agaaacggaa aaaaaagaag ttttacaagc
     9841 aataaaaata ttaataaatt gtattcatct ttgtttttac aagcaagtgc atttttacag
     9901 caataccgac aattattaag tgattcagct actaatctta ttcagaactt tttaaatctg
     9961 cagactgcag gatttctaaa acgcaagtac ttgattattg ccaattccct ttatcgccct
    10021 acttggccta aaaccctctt tttcatattg cgattttgat ttttcgtacc tttatcccca
    10081 gcatgcagcc caaattagcc gtcatcatgt taacatacaa cagcgcaaaa catttgcctg
    10141 ttttttttca atgcatggct aagcaaacct acaaacaata tactttgttt atcctagaca
    10201 atcattcaga ggataattcc gtagcaatta ccgaacaaca agaattgccc attgtgctga
    10261 taaaaaatga aactaatgtc ggatttgcaa aaggaaacaa tctcgcaata gcaatggcgc
    10321 tcgatgctga ttatgaattt atcttactgc taaataatga catcgaatgt gatgagtttc
    10381 tttttgaaaa catggtgcaa aaattaaccg cctctgaaga acatcagttg ctgtctccca
    10441 aaatattatt ttaccctcaa acggaatata tctggtatgc aggatgcctt tttgataaat
    10501 acaaaggcaa taatactatt atcagaggtc tgtatgaaca tgacaatggg caatatgaca
    10561 cacccgaaac gatggatttt gctcctgcat gctgcttatt gtttcatcgg aaagtactgg
    10621 aaacagtcgg tctgtttgat gaaaaatatt ttgcgtatta cgaagacgcc gatttacttt
    10681 ttcgggttaa aacacagagc aagtataaat tgttatatag tcctgatata aagatgtatc
    10741 ataaagtagg cggttcgttt aatgccaggc aggatttaaa aaatgtaaat caattcactt
    10801 ttggcaatca atacattcat cttacaacaa gaaatttcgt ctattattta ctgaaacaaa
    10861 aattgttttg gtcttattgt tacttattgg tcttcttttt ccgcatgcaa gctcgttttt
    10921 tctttagtgg caagtataag gtcaatttga agacgtatcg gttactttta agatcctatt
    10981 ttgaggggtt tgttttataa aagtaattca taagtaagaa tatacttagt aacctgagtt
    11041 cgggataaga aaattaaggt gaaaaaaata gttgatttct tattttttcc tccaacttaa
    11101 caaggaggag gaattaaaag gaggtggttc tatata
//
LOCUS       DAOQII010000059        10978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3056_length_10978_cov_5.158931, whole genome shotgun sequence.
ACCESSION   DAOQII010000059 DAOQII010000000
VERSION     DAOQII010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_10978_cov_5.158931"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="asnB"
                     /locus_tag="PLF48_04370"
     CDS             complement(<1..1354)
                     /gene="asnB"
                     /locus_tag="PLF48_04370"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNF48593.1"
                     /translation="MCGIAGAYSFLQKPIAEKWLHDAAVSMNKRGPDNQNFVVIQKAA
                     LVHARLSIIDTSVVANQPIYDSSSRYAIIFNGEIFNFLSLRKELENSGVVFKSHSDTE
                     VLLYAYIKWGKDALHKLNGFFAFAIYDKQEDHLFIACDRYGIKPLYFYKNDDFLFFGS
                     ELKAILSFPVTKNIDWSAVSLYFQMNYIPGKASVFKNTYRLESGHFIEIKNNTTQIGK
                     WYSIPYHALEAEAQPLSYEAQQKKLVELMDDAVRLRLIADVPLGAFLSGGVDSSVVCA
                     MAARHTPHLNTFSIGFEGNQFFDETPYADLVAKHLKTNHTVFKLKNDDLLQSLNELLD
                     YTDEPFADSSALLVNILSKHTRKHVTVALSGDAGDELFAGYNKHYGEWQIRNGGWKAQ
                     AIHLLKPLWEKMPKSRSGFLSNRIRQLERFANGMDLSPAERWWQWCTFFGLDNVNKLL
                     N"
     gene            complement(1466..2515)
                     /locus_tag="PLF48_04375"
     CDS             complement(1466..2515)
                     /locus_tag="PLF48_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48594.1"
                     /translation="MKTSYKTPILFGLAHGINDFIAGYLLSAHTASGASIEKNTFVFL
                     MYGIIAFGGQLFTGLVVDKTRQLKKATLLSVLLMICAIGVFNSNLTIAILISGLASAL
                     IHVCGGAACFFDDNESNSMVGIFTSPGVIGLIVGGILGIHPSAYFYALIPLLTGIFYL
                     VSTLNIGYKNEIKQQRFSISFETHDYVMLLLLLAISLRSLMWNMMQLLTHNETEKLIG
                     IAIAAFMGKLFGGSLCDRVSWKKFVYITTFLSAIFLTVGKTYQPLFYIGIACLQSGIP
                     ITLVLMQNLLRTMPATASGLALGVALVLSGLPMYVNQFRLLQDKTSIFILVLLLFVLG
                     NWLAIRKYANGVKET"
     gene            complement(2512..2715)
                     /locus_tag="PLF48_04380"
     CDS             complement(2512..2715)
                     /locus_tag="PLF48_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48595.1"
                     /translation="MLTWPILNLLYFNTNINLLILESGVTLVEAIIIYLFLNQKPAKA
                     LLISFVQNAVSTAIGVYLNGIKL"
     gene            complement(2813..3391)
                     /locus_tag="PLF48_04385"
     CDS             complement(2813..3391)
                     /locus_tag="PLF48_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48596.1"
                     /translation="MKKRFLLLLLLGFFGIAKSNMPPPMYEITTALKFTHINQYPDVD
                     FYILFNDNKKNKLAENKTYILNANKTTRTSRQQFSCRVFFQNKSNKAIADTIMLYGER
                     SYGYNIDYYMQKTIQLSIENNKIGYDEIEVKKVNNTASKTKKQKSNTFMSFPTANGNG
                     GNTLLIVLSFLSMLVFTLLFLFRQKRLTISNV"
     gene            complement(3388..3528)
                     /locus_tag="PLF48_04390"
     CDS             complement(3388..3528)
                     /locus_tag="PLF48_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48597.1"
                     /translation="MNNSILLDAGGPVLVAGFVAFVMIIAALVFFSVLLLYKYLKNRS
                     KK"
     assembly_gap    3742..3841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4041..>5021)
                     /gene="gpmI"
                     /locus_tag="PLF48_04395"
     CDS             complement(4041..>5021)
                     /gene="gpmI"
                     /locus_tag="PLF48_04395"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNF48598.1"
                     /translation="CGRYYAMDRDKRWERVEKAYDLYVNGKGSAFDTAEAGIKSSYEN
                     GITDEFVLPFFIQDENKQPLARIQEDDVVICFNFRTDRCREITTVLTQTDMPEYNMHT
                     LPLYYVTMTVYDEKFKDVKTVFGKENVTDSLGEILSKNNRTQLRIAETEKYPHVTFFF
                     SGGREDVFEGEERLMIPSPKVATYDLQPEMSAYEVRDAVIKYIDENAPDFICLNFANS
                     DMVGHTGVFAAAIKAVETVDACVQDVVTKALEKDYAVLLTADHGNSDYMVNYDGSPNT
                     AHTKNVVPLFLIDNHLHPVLQPGKLSDIAPTVLKIMDIPIPKAMNGTVLF"
     assembly_gap    5022..5121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5207..5599
                     /locus_tag="PLF48_04400"
     CDS             5207..5599
                     /locus_tag="PLF48_04400"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNF48599.1"
                     /translation="MRWYKYLSVLTLSLLFSLSVVAQDVFTVFPAALKSGNITAISKN
                     FDKRIDITIDDNSDNYSKDQAEVILKDFLSKFSTREFTVIHKGASPNGAQYVVGNLKT
                     NKGSYRTYIYVKNTDGVSYIQEIRFEKE"
     gene            5650..6501
                     /gene="nadC"
                     /locus_tag="PLF48_04405"
     CDS             5650..6501
                     /gene="nadC"
                     /locus_tag="PLF48_04405"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNF48600.1"
                     /translation="MIDQQELEQFIRLALYEDVRGGDYSSLAILDKKTQGKARLLVKD
                     EGVLCGMPAARKIFEMVDADLRVEEYIAEGNNIHYGDVAFHVYGSAISILTAERLVLN
                     CMQRMSGIATKTQEYVKKIAGTSAKVIDTRKTTPGIRFLEKYAVTVGGGANHRMGLYD
                     MIMLKDNHIDFCGGITKAVHKVRNYLQENNLSLRIEVETRNLDDVKEVLACGGIDRIM
                     LDNFTPEKVQEAVGLINNRYETEASGGITLETIRSYAETGVNFISVGALTHSVRSLDL
                     SLKAEIG"
     gene            complement(6710..10936)
                     /locus_tag="PLF48_04410"
     CDS             complement(6710..10936)
                     /locus_tag="PLF48_04410"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF48601.1"
                     /translation="MLFDANAQCSGVSILPVKASYCAGEPITVSVISPTLGITYRIVI
                     NGDVYPDTVAVVGLPGAATTRDYYIDVQFINPLGQWSSCQPIPPLRPKVTIAPSPDPT
                     LIDISPNKSGEFKQCSSVSPYQFVVQNGSSTASAYTQTYLIDWGDGTNTGVMNVFSSS
                     LTHNYPAPGYYDVKFTVTSAQLSPPCNTVTQTYKVYFGVLPNIGGIGRLDPTIKCVPA
                     IEQFLIDTTLTNRNSPGTEYSVKVDGVAVANFSQPIPRSFNYTFTKTSCGKSCGAGLE
                     NFEVIVTAKNLCGVSQSTVCVGENEKPKPQLQVRDTVCLNTPTNHTNATVQKELQGTG
                     KCNPLPTTWHISPHTWTLNSGDTLSSSTIVVSYNQTGTYTVNMTVESVCGDSSISKQV
                     VVVEKVKALAKFVSPPCIPASGFVDVPISNFSTRPPSARGYTWTITPNTGVSFVSGND
                     HSDSVTVRFTISGSYNVHLAVNGACNNAVWDSTLVIKGKPVIDTSRIPEACFFPATLN
                     PNNYFTFNNGGDPNATFAWTFTGGNPASASTANPGTINYASSGTFPILLHITNICGDS
                     SLTNSFTVNNYPKPFAGNDTGICIGTPSFQLHGTPAGGIWSGGHIDSTGVFDPAGLGR
                     GNYPVIYTTNQTSSCPTRDTLLVKISKLDSISLAPQTVCKSTRAVILAGSATYPNGNW
                     SGPGVVNSTTGLFDPVGLIPGFHDIYYTYQDSSGACTKTVIKRVNVLDSVWIGTPPVL
                     CVNQSFDFGTISGNIQTATWKFGDGTPNAIIPNPSHIYASAGNFTVTLYAETQDHCKD
                     TILIPVKVIKNPPLSFLTSNYDSCTGNAVTFSFPPTHDTATHYAWSFGVSSLEENLPN
                     THIINFPPPRSGDSTYIITLRADYYCGPVYFSDTIKVKSKPKADFQVQPIGCSPFKPT
                     VLNQSYGSPTSYIWDFGNGITSTLQNPVVPTYINNTPRDTTYTIKLKVSNTCGTDSTS
                     ETFVLKGNNVNADFFTNITQGCRPLNVNFYSISTPGVQLIWDFGDGQQGFAEELIHTY
                     DTSGVFRVKLLAVGSCGRDSAFTNINVFPKPKANFTVKNPCVGKTTQFINASTNGNSF
                     IWDFGDGSAHSSLQNPTHVYTVVGPYVVKLIVSNANGCVDSIETTIGVTTQPTAGFAV
                     EDLDLCQNEPVILRNTAQNGNNFIWYFGNGETSTDVVPNYTYPNPGTYTITQVAINGA
                     CRDSLVRPAAIQIHPTPLAEFIAELTANKFNDPVVFNNQSSNANSYIWLFGDGDSSVV
                     ADPTHLYTGDGPYRVTLYAVSSFGCKDTVSHDIGVDSSGVMYIPNVFAPEIGSGEGAV
                     FLPKTKNLVEYRLQIFSTYGQLLWESTALKEYGMPAEGWDGRFKGTLMPQDVYVWKIF
                     GIFENGKVWDGMVDPKTGKKSVMGAVLLLR"
BASE COUNT         3391 a         2082 c         2066 g         3239 t
ORIGIN      
        1 cgttcaatag tttatttaca ttgtccaaac cgaaaaacgt gcaccattgc caccaacgct
       61 ctgccggcga taaatccatg ccgtttgcaa atcgttccag ttgacgaatc ctgttggata
      121 agaaaccgct cctggatttg ggcatctttt cccacaatgg ttttaataga tggattgcct
      181 gcgccttcca accgccgttt cttatttgcc attcgccata gtgtttgtta tatcctgcaa
      241 aaagctcatc tccggcatcg ccactaagtg caacggttac atgcttgcgt gtgtgttttg
      301 atagaatatt taccaacaaa gccgaagaat cggcaaaggg ttcatctgta taatcaagca
      361 attcattcag actttgcaat aaatcgtcat tctttaattt aaaaaccgta tgattggttt
      421 ttagatgttt ggccactaaa tcggcataag gcgtttcatc aaaaaactga ttaccttcaa
      481 atccaatgga aaaagtattg agatgcggag tatgccgtgc tgccatggca caaaccactg
      541 atgagtcaac acctccgcta agaaaagcac ctaagggcac atctgcaatt aagcggaggc
      601 gaacggcatc atccatcaac tccactaatt ttttttgctg tgcctcataa ctgagtggct
      661 gtgcctctgc ttccagtgca tgatacggta tggagtacca tttgcctatt tgagtagtat
      721 tgttttttat ttcaataaaa tgaccggatt ccaagcgata ggtattttta aaaacagatg
      781 cttttcccgg aatataattc atctggaaat aaagacttac agcactccag tcaatatttt
      841 ttgtaacagg aaatgacaag attgctttta attccgaacc aaaaaaaaga aaatcatcat
      901 tcttgtaaaa gtagagtggt ttaatgccgt atctgtcgca agcaataaat aaatggtctt
      961 cttgtttgtc gtaaatggca aatgcaaaaa aaccattcag tttgtgcaag gcatctttcc
     1021 cccattttat ataagcatat aataaaactt cggtatcaga atgcgatttg aaaacaacgc
     1081 cagaattttc aagctctttg cgcaacgata aaaaattaaa aatttcgccg ttgaagataa
     1141 tggcatatct gctgctgctg tcgtatatgg gctgattagc cacaactgat gtatctataa
     1201 tgctcaggcg ggcatgtacc aatgccgctt tttgtatgac tacaaaattt tgattatcgg
     1261 gtccgcgttt attcatagaa acagctgcat catgcagcca tttttcggct atgggttttt
     1321 gaagaaatga atacgcacct gcaattccgc acatagccac aaattccgca aaaaaaatca
     1381 aaaatcaaaa taccgagaat tacgaaacaa aaatcaaatt gataatttga atacataata
     1441 tggatggcta tagcttatta acaaactaag tttcttttac cccgtttgcg tactttctaa
     1501 ttgctaacca gttgcctaat acaaataaca gcaatactaa aataaaaatg cttgttttgt
     1561 cctgtaatag ccggaattga ttcacataca tcggcaagcc tgacaatact aaagcgacgc
     1621 caagagctaa gccggaggct gttgccggca tcgtgcgcag aagattttgc atcaacacta
     1681 aagtaatggg tataccgctt tgcaaacagg caatgccgat ataaaatagg ggttgataag
     1741 tcttaccaac agtaagaaat atcgcagaaa gaaatgtggt gatatataca aatttcttcc
     1801 agcttactct atcgcaaaga gagcccccga acagcttgcc catgaaggct gctattgcta
     1861 tgcctattaa tttttctgtt tcattatgcg ttagcaattg catcatattc cacattaaag
     1921 aacgcaagct tattgccagc aatagcaaga gcatgacgta atcatgggtt tcaaaggaga
     1981 tagaaaaacg ctgttgttta atctcatttt tatagccgat attcagcgtt gaaaccaaat
     2041 aaaagatacc ggttagaagt ggaatcaatg cataaaaata tgcgctggga tgaatgccga
     2101 gaatgcctcc aacaattaaa ccaatcactc cgggcgatgt aaaaataccg accattgaat
     2161 tgctttcgtt gtcgtcaaag aaacaggctg ctcctccgca aacatgtata agcgcagaag
     2221 ctaaaccgga aatcaggatg gcaattgtca gattagaatt aaatacacct attgcgcata
     2281 tcattaacaa tacagacaat aaagtagctt ttttcagttg acgagtttta tcaaccacta
     2341 ggcctgtaaa taattgcccg ccaaatgcga ttataccgta catcaagaaa acaaaggtat
     2401 tcttttctat gcttgccccc gatgcagtat gagccgataa taaatagccg gcaataaaat
     2461 catttatgcc atgtgcaagt ccgaatagta tgggtgtttt gtatgaagtt ttcatagttt
     2521 tatcccattc aaataaaccc caattgctgt agataccgca ttttgtacaa atgaaatcaa
     2581 caatgcttta gcaggctttt gatttaaaaa cagataaatg ataatagctt caaccaaagt
     2641 taccccactt tctaaaatta gcagattaat attcgtgttg aaatagagca agtttaggat
     2701 tggccacgtc agcatgttgg caaaaaacaa aattaaaatg gtacgggtta tttgctttct
     2761 aacaaaaata aagtaaatcg gaatctcgat gagcagagta taaaagaagt aattaaacat
     2821 tcgatatcgt tagccgtttt tgtctgaata aaaacaatag agtaaacact agcatgctga
     2881 gaaatgacaa tacaatcagt aacgtatttc ctccattgcc gttagcagtt ggaaaagaca
     2941 taaacgtgtt tgatttttgt ttttttgttt ttgatgctgt gttgttaacc ttttttactt
     3001 caatttcgtc atagcctatc ttattatttt ctatgctgag ttgtatggtc ttctgcatat
     3061 aataatctat gttgtaacca taagatcttt cgccatacag cattatggta tcagcaatgg
     3121 ctttattact tttattctga aagaagacac gacaggaaaa ttgctggcga gaagtacgag
     3181 tagttttgtt ggcatttaga atgtaagttt tattttctgc aagtttgttt tttttgttgt
     3241 cattaaacag aatataaaaa tcaacatcgg gatactgatt aatatgggta aatttcaagg
     3301 ctgtagtaat ctcatacatg ggtggaggca tattgctctt tgcaatgcca aagaaaccca
     3361 acagcaatag caacaagaaa cgctttttca ttttttactt ctgtttttta aatacttata
     3421 caatagcaga acggaaaaga agaccaatgc agctattatc atcacgaaag caacaaagcc
     3481 agctactaat accggtcccc cggcatctaa gagaatcgag ttattcatgg ttttctgatt
     3541 tatctgattt agtaccaaat cctgccaact tccaaaatac aaataccaat agtcctactc
     3601 ccagtagcga cattagcatt aatacattaa gcatgctcat gattcctgat tttttttacg
     3661 tttataaaat tgatagataa aaaaagtaat agcaacagaa cttaatataa ccagggtaga
     3721 ggtaatattt cgccatttcc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 ncttgcataa aacaaataat ccggattttc tgttatcccc ttcacccgaa tgtcaaaaga
     3901 tggtcgtgct ttatttccgg gcatatcagc atacaaatgc acgataagca ataagcatat
     3961 tgctgtgtat atggttttca taaattaaaa ataagcaatt aatgtttgat ttgccaaaaa
     4021 attatcacaa gtaattggta ttagaataat accgttccgt tcattgcctt aggaatagga
     4081 atatccataa tcttcaagac ggtaggcgca atatcagaca gtttgcctgg ctgcagcacc
     4141 ggatgcagat gattatcaat taaaaacaag ggtacaacat tttttgtgtg tgctgtattt
     4201 ggtgagccat catagttcac catataatcc gagtttccgt ggtcggctgt gagcaaaaca
     4261 gcataatctt tttccaatgc ttttgttact acatcctgta cacatgcatc caccgtttca
     4321 accgctttta tagcagcagc aaacacaccg gtatgcccaa ccatatcact gttggcaaaa
     4381 ttcaagcaaa taaaatctgg cgcattttca tcgatatatt ttattacggc atcccgcact
     4441 tcataagcac tcatctccgg ttgcaagtca taggttgcta ctttcggcga aggaatcatt
     4501 agtcgctctt ctccttcaaa cacatcctct ctgcctccac tgaaaaagaa ggttacatgc
     4561 ggatattttt ctgtttcggc aatgcgcaat tgcgtgcgat tatttttaga taaaatctca
     4621 cccaaagaat ccgttacatt ctcttttcca aatactgtct ttacatcttt gaatttttca
     4681 tcataaaccg tcattgttac ataatacaaa gggagtgtgt gcatattata ttcaggcata
     4741 tcggtttggg tgagtactgt tgtaatctca cggcaacggt cagtacggaa gttaaaacaa
     4801 atcactacat catcttcttg tatgcgtgca agaggttgtt tgttttcatc ctgaataaag
     4861 aaaggcaaga caaattcatc tgttataccg ttctcataag aagattttat tccggcttct
     4921 gccgtgtcaa atgcactacc cttaccgttt acgtataagt cataagcttt ctctacccgc
     4981 tcccagcgtt tgtctctgtc catggcataa tatcttccgc annnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nacttgttaa tttttatgta aaaaatatag catttttgct
     5161 tactttggca caataaatgc aataaccaat acaaaagtac aattttatgc gctggtataa
     5221 atatttatct gtattaacat taagtctgct cttcagcctg tctgtcgtgg cacaagatgt
     5281 ttttacggta tttcctgcag cactaaaaag cggaaatatt acagctattt ctaaaaattt
     5341 tgacaaacgc attgacatta cgattgatga taattctgac aactacagca aagatcaggc
     5401 agaagtaatc ctcaaagatt ttctaagcaa gttctccact cgcgagttta cagttatcca
     5461 taaaggtgca tctcccaacg gggcacaata tgtggtaggc aatctcaaga cgaataaagg
     5521 atcttatcgc acgtatattt atgtaaaaaa tacggatggc gtgagctata tccaagaaat
     5581 tcgctttgag aaggagtaaa attcccattt ggcatatttc ttattgtgta tttcatttac
     5641 ctttgatgta tgatagatca acaggaattg gaacagttta tccgtttggc attatacgag
     5701 gatgtgcgtg gcggagatta ttcgtcatta gccattttag ataaaaagac acagggcaaa
     5761 gctcgattac tcgtaaaaga tgaaggagta ttgtgtggaa tgcctgctgc acgcaaaata
     5821 tttgagatgg tggatgcaga tttgcgagtt gaagaatata ttgcagaagg caataatatc
     5881 cactatggtg atgtagcatt tcatgtttat ggcagtgcta tttccattct cacggcagaa
     5941 cgattagtat taaactgtat gcagcgtatg agcggaatag caacaaaaac tcaggagtat
     6001 gtcaaaaaaa ttgcaggaac atcagcaaaa gtaattgata cccgcaaaac cacacccggc
     6061 ataaggtttt tagagaaata cgcggtaact gttggaggag gagccaacca ccggatgggt
     6121 ttatatgata tgattatgct caaggataat catatcgact tttgtggagg cattaccaaa
     6181 gctgtacata aggttagaaa ttatttacaa gaaaacaatt taagtcttcg tatcgaggtt
     6241 gaaacccgga acctggatga tgtaaaagaa gtattagctt gtggtggaat tgacaggatt
     6301 atgctggata attttacgcc tgaaaaagtt caagaggctg ttgggctgat taataatcgc
     6361 tatgaaacag aagccagtgg cggtataacg ctggaaacta ttcgcagtta tgcagaaacc
     6421 ggagttaatt ttatttctgt aggcgcatta acacactctg tgcgcagttt agatttgagc
     6481 ctgaaagcag aaataggcta agttaataac ctgagttctg gttaagaaaa ttcaggtaaa
     6541 aaaaatagtt gatttctcat tttttcctcc aactttacaa ggaggaggaa ttaaaaggag
     6601 gtggttctat ataagaaatc aactattttt taattgaaat acttatcccg aactcaggtt
     6661 aataatagct gaattagtgg agtctcggca caattcaaat tcgtagatct tatctcagta
     6721 agagtacggc tcccatgacc gattttttgc cggttttcgg gtcaaccata ccatcccaca
     6781 ctttgccatt ttcaaagatg ccgaaaatct tccagacata cacatcttgc ggcatcagcg
     6841 tacctttaaa tctgccgtcc cagccctcgg caggcatacc gtattctttc aatgcggttg
     6901 attcccaaag taattgacca tacgtagaaa agatttgcaa gcgatactct accaaatttt
     6961 tggtttttgg taaaaatact gccccctcac ctgaaccgat ttcgggagca aaaacattcg
     7021 ggatatacat caccccacta ctgtcaaccc caatatcatg cgaaacagtg tctttgcaac
     7081 caaatgaact gacggcatat aaagttacac gatatggacc atctccggta taaagatgcg
     7141 tcgggtcagc tacgacagac gagtctccat caccaaataa ccagatatag ctattggcat
     7201 ttgatgactg attattaaag acaaccggat cattaaattt attagcagtc agttcagcaa
     7261 taaattcagc aagcggtgta ggatgtatct gaatggcagc agggcgaacc aaactatcac
     7321 gacaagcacc atttattgcc acttgtgtta tagtatacgt tccggggttt ggataagtat
     7381 aattcggcac tacatcagtt gaagtttcac cattgccaaa ataccagata aaattattgc
     7441 cattttgagc agtatttctc aaaattacag gttcgttttg gcataaatcc aaatcttcta
     7501 cagcaaaacc ggcagtaggt tgggtagtaa caccgattgt agtctcaatt gaatcaacac
     7561 aaccatttgc atttgataca atcagtttta caacatatgg gcctactaca gtataaacat
     7621 gtgtaggatt ttgtaagctg gaatgagctg aaccgtcacc aaaatcccag ataaaactat
     7681 tgccgttggt agaggcattg ataaattgag tggtcttgcc tacacatgga ttctttacag
     7741 taaaattggc cttaggtttt ggaaacacat tgatattcgt aaaagcagaa tctcttccgc
     7801 atgagcctac tgcaagtaat tttactctga atactcctga tgtatcatat gtatgaatta
     7861 attcttcggc aaagccttgt tgtccgtcgc caaaatccca tattagctgt acacccggcg
     7921 tagatatact gtaaaaattc acattcaatg gtcggcaacc ttgtgtgata ttcgtaaaga
     7981 aatctgcatt tacgttatta ccctttaata caaatgtttc tgaagtgctg tcagtgccgc
     8041 aagtattcga tacttttaat ttgatagtgt aggtcgtatc tcgtggggta ttgttgatat
     8101 aggtaggcac taccggattt tgtaaagtag aagttattcc gtttccgaaa tcccagatat
     8161 aagaggttgg agagccgtac gactggttga gaacagtagg tttaaaagga gagcatccga
     8221 tgggctgaac ctgaaaatca gcctttggtt ttgattttac ttttatcgta tctgaaaaat
     8281 aaacgggacc acaataataa tcagcccgaa gtgtaatgat ataagtagaa tcgcctgaac
     8341 gaggcggagg aaaattaata atatgcgtat tcggtaaatt ttcttctaaa gatgaaacac
     8401 caaaactcca agcgtaatgg gttgccgtat cgtgtgttgg aggaaaactg aaggtaactg
     8461 catttccggt acaagaatca taatttgatg tcaggaaaga cagtggcgga ttttttatga
     8521 ctttaacagg aatcagtatc gtgtctttac agtgatcttg cgtttctgcg tataaagtaa
     8581 ccgtaaaatt gccggcactt gcataaatgt gagatgggtt gggaatgatg gcatttggcg
     8641 taccgtctcc gaatttccag gttgcggttt gaatatttcc gcttatcgta ccaaaatcaa
     8701 acgattgatt tacgcataac acaggtggtg tacctatcca gacactatcc agtacattta
     8761 ctcttttaat gacagttttt gtacatgccc ctgatgaatc ctgataagta taatagatgt
     8821 catgaaatcc gggtattaat ccaacaggat caaataaacc tgtagttgaa ttaacaacac
     8881 ccggaccact ccagtttcca ttcgggtatg ttgcactacc ggctaaaatt acagcacgtg
     8941 tagatttaca aactgtttga ggagccaaag aaatgctgtc taattttgaa atttttacca
     9001 ataatgtatc tcgtgtcgga caacttgatg tttgattggt tgtataaata acaggataat
     9061 ttccccttcc caatcctgcc ggatcaaata cccctgtgga gtctatatgt ccaccgctcc
     9121 aaataccacc tgcaggagtt ccatgcaatt ggaaagatgg ggtaccaata caaataccgg
     9181 tatcatttcc ggcaaaaggt ttgggataat tattcacggt aaatgaattt gtcaatgaag
     9241 aatcaccgca aatatttgta atatgaagta gaatcggaaa tgttccggat gatgcataat
     9301 ttatggtgcc tggatttgct gtggaggcag atgcaggatt tccccccgta aaagtccagg
     9361 caaaggtagc attggggtct cctccattat taaaggtaaa gtaattattg gggttaagcg
     9421 tggccggaaa aaaacatgcc tccggaatac gggaagtatc aattacgggt tttcctttta
     9481 taaccaatgt gctatcccaa acagcattgt tgcacgcacc atttactgct aaatgtacat
     9541 tataactacc tgatatagtg aaccttaccg ttacactatc tgaatgatca ttgcccgata
     9601 caaacgatac tcctgtattg ggtgtaattg tccaggtata accgcgtgcg gatggcggac
     9661 gggtagaaaa gttggatata ggaacatcta caaatccact tgcaggaata caaggcggcg
     9721 aaacaaactt ggctaaggct tttacttttt ctactactac aacttgtttg gatattgagc
     9781 tatctccaca aactgattct acagtcatgt ttactgtata agtacctgtt tgattatacg
     9841 acactactat ggtagaactg ctcaatgtat ccccactgtt taatgtccat gtatggggtg
     9901 aaatatgcca tgtggttggc aatggattac atttacctgt tccttgtagt tctttttgga
     9961 cagttgcatt tgtatgatta gtaggagtat taagacaaac cgtatctctt acttgcaatt
    10021 gtggtttagg tttttcattt tcccctacac ataccgtaga ttgtgaaaca ccacaaagat
    10081 tctttgctgt tacaattact tcaaagtttt ctaatccggc tccgcaactc ttgccacatg
    10141 aagtttttgt aaaagtataa ttaaacgatc tcggaatagg ctgagaaaaa ttagccactg
    10201 caacaccgtc aactttcacg gagtactctg tacccggaga gttccggttt gtcaatgttg
    10261 tatctatcaa aaactgttct atagcaggta cgcattttat tgtggggtct agcctgccaa
    10321 taccaccaat attaggcaat accccaaaat atactttata agtttgtgtt actgtattac
    10381 aagggggact taattgagcc gaggttactg taaattttac atcataatat cccggagccg
    10441 gataattatg tgttaaagaa gaagaaaaca cattcattac tcctgtattt gtaccgtcac
    10501 cccagtctat tagataagtc tgcgtataag ctgatgcagt actggaacca ttttgcacaa
    10561 caaactgata tggcgacact gaagaacact gtttaaactc accagattta tttgggctaa
    10621 tatctatgag tgtcggatct ggagaaggtg caattgtaac tttgggtcgc aaaggtggaa
    10681 tcggctgaca tgaactccat tgtccaagcg gattaataaa ttgaacatca atataataat
    10741 ctctggtcgt agctgcacct ggcaacccga ctactgctac cgtatctgga taaacatctc
    10801 catttattac tatcctgtaa gttatcccta gtgtaggact aataacactt acagtaattg
    10861 gttctccggc acaatatgat gctttaaccg gtaatatgga tacgccgctg cattgtgcat
    10921 tagcatcaaa aagcacaaat acagtaagta ttaggagcag taatatcttg taaagatg
//
LOCUS       DAOQII010000060        10824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3126_length_10824_cov_4.738973, whole genome shotgun sequence.
ACCESSION   DAOQII010000060 DAOQII010000000
VERSION     DAOQII010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3126_length_10824_cov_4.738973"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..692)
                     /locus_tag="PLF48_04415"
     CDS             complement(24..692)
                     /locus_tag="PLF48_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48602.1"
                     /translation="MKQNLTIENVIIEAKAFCIAQSKTPNNELYGVTDGKAVGTHIEH
                     KFQQYLAEKYELKIGSSAKGIDLPSVDIDIKVTSIKQPQSSCPFKDAKQKIFGLGYNL
                     LVFVYDKIDNPKTKTANLDFVSCSFISKERTADYTTTYRLREMVKDNANIEDIVAYLN
                     DKNIPADEIMLQQLAEQILKTPPEQGYLTISNALQWRLQYQRIVTMAGNINGINKIID
                     KMKH"
     gene            complement(984..2453)
                     /locus_tag="PLF48_04420"
     CDS             complement(984..2453)
                     /locus_tag="PLF48_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNF48603.1"
                     /translation="MAKLKLQGKDLRKINYPEDRTIAIVIDVMHKHFKHYTLECALIL
                     LKEILDNPFEFSENEPLSKIAQALIDYNMKCLIKGETDKMTIATELLQDRLEQPARHF
                     AFRSDTLDYASFGMNHIEEGALKQMETAMKLPVTAAGALMPDAHQGYGLPIGGVLATE
                     NAVIPYGVGVDIGCRMSLSVFAVSEKMLKSKQAYFEKILKENTLFGAGKEFVKSADHA
                     ILDRVEFNEIPMLKSLQLKAAKQLGSSGSGNHFVEFGIVEITDAANNMHLPIGKYLGL
                     LSHSGSRGLGATVANHYTKLAMERCKLPQESRHLAWLSLDTQEGLEYWQAMNLSGDYA
                     SACHHTIHYKIAKELNENAISVIENHHNFAWKEVHNGKQVIVHRKGATPAGKNVMGII
                     PGSMTAPGYIVSGKGNEASLHSASHGAGRKMSRSKAQSSISMSEMKKLLSENGVTLIG
                     GGLDEAPQAYKDIQKVMQAQTDLVQVEGLFYPKIVRMDG"
     gene            complement(2569..2641)
                     /locus_tag="PLF48_04425"
     tRNA            complement(2569..2641)
                     /locus_tag="PLF48_04425"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2605..2607),aa:Phe,seq:gaa)
     gene            complement(3394..3467)
                     /locus_tag="PLF48_04430"
     tRNA            complement(3394..3467)
                     /locus_tag="PLF48_04430"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3431..3433),aa:Lys,seq:ttt)
     gene            complement(3815..3889)
                     /locus_tag="PLF48_04435"
     tRNA            complement(3815..3889)
                     /locus_tag="PLF48_04435"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3853..3855),aa:Asp,seq:gtc)
     gene            complement(4534..6060)
                     /locus_tag="PLF48_04440"
     CDS             complement(4534..6060)
                     /locus_tag="PLF48_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HNF48604.1"
                     /translation="MKFNIKSVFTTKVKNYEKADAYTMSPELELYAAVVTASLSDTFY
                     EKEQERMERIRALIAKVDTLFVAKLAAYTRQKMNLRSASLVLAVELAKRANGNSIVKN
                     TVNQVVQRADEITDLLAYYELANKRTGTKRLNKLSKQIQKGLAMSFNKFDEYQFAKYN
                     RDAAIKLRDALFLVHPKAKDEAQQILFDKIAGNSLTVPYTWETNLSVLGQTKFETEQA
                     KKAAFGNKWEEMIASGKIGYMAMLRNLRNILEAEVSYNSIEKICAYLSNAENVAKSKQ
                     LPFRFLSAYRELKAIPSGCTSNVMRAIEDAVKASAVNIKGYDADQRIVLACDVSSSMQ
                     KPVSARSKIMLYDIGLLLAMLLQSKSKNVITGMFGDRWKIINMPTGNILANVDEFYRR
                     EGEVGYSTNGYLVIEDLIAKNKIVDKVMMFTDCQLWDSRNSGSSMENSWNRYKRVAPN
                     AKLYLFDLAGYGNTPLNLVRHDIFLIAGWSDKIFDVLAAIENGENALSEIKKMSIGVV
                     "
     gene            complement(6184..7266)
                     /locus_tag="PLF48_04445"
     CDS             complement(6184..7266)
                     /locus_tag="PLF48_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF48605.1"
                     /translation="MEFVQNKYAIDVNRIFNGGDYLDAKALFWALNGVIPTMHSIYPI
                     DVDKALKFFEANYKDEIDTLLKSSNLNDNNNMKGFELDEVNIVLKDKSVLKITYNYCD
                     ILTRGDKQEWIESLSSKLKKFRRREKKKEFELNLISVNGNNFSLKSIDVKKTRLNLDL
                     YYEDEFLETDKIIRERLNRNKDKGIVLLHGLPGTGKTTYLRYLIGKVKKRVLFVPPSV
                     AGNITNPEFMNLLLEYPNSILIIEDAENIIMDRQITGSSAVSNLLNISDGLLGDCLNT
                     QIICTFNMPISKIDTALTRKGRLIAKYHFGKLSVAKAQQLSDKMKLGVFVTEPMSLAE
                     LFNTHEKDSEDTSFRVLGFKRVQVLN"
     gene            7812..8348
                     /locus_tag="PLF48_04450"
     CDS             7812..8348
                     /locus_tag="PLF48_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48606.1"
                     /translation="MESVNNNYSDLSELEQKIQKSLERVRDEYTQLDVNLKNQKWITN
                     KIKEYLGELGSSFGYEISSSFHNQEWLYDVVWYKNNENNILEKTILVVESELSNRSIN
                     SLKYDFEKLLLSNSDLRVMICFGQGNFNFPQNVNGIIESFNNYVTTYQNLNSGDRILI
                     LVWDDYMTGEVYPHLIIK"
     gene            complement(8371..9042)
                     /locus_tag="PLF48_04455"
     CDS             complement(8371..9042)
                     /locus_tag="PLF48_04455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNF48607.1"
                     /translation="MIYKFITIEGNIGAGKTTLASLLAEEYHGQLILEAFEDNPYLPK
                     FYEDNAKYALQLEMSFLIERYQQLTRIFSEPNIFSNFTVTDYMLKKCLLFAKVNLTKQ
                     EYKLYKHFFDQIYKKLPKPEIIFYLHAETDQLVRNIRKRGRDYEQNISRVYLNRLERM
                     YFEYFKQNPDFKYVIIDVNGIDWVSNIFAYRQLLKLFDQEYKTGMNFVKLEQENLLFE
                     EQAIS"
     gene            complement(9039..9527)
                     /gene="folK"
                     /locus_tag="PLF48_04460"
     CDS             complement(9039..9527)
                     /gene="folK"
                     /locus_tag="PLF48_04460"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNF48608.1"
                     /translation="MNTVYLLTGSNLGHRYKNLLLALYFIKEEVGTITKMSAIYESPS
                     WGVLHKTKYLNQAICVQTGLSPYQLLHKLQKIEKYLGRKNKKQNAPRTLDIDVLFYND
                     LIFKTSDLEIPHPRLHLRRFTLLPLSEIAKSYTHPESGKSIEELLNSCKDDAKVSIFK
                     PE"
     gene            9601..>10824
                     /locus_tag="PLF48_04465"
     CDS             9601..>10824
                     /locus_tag="PLF48_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNF48609.1"
                     /translation="MRNFIKMFFACLLAIGFSFLFFFIIFTMLASLGSQSSKKSGAKA
                     NSVLNLTLKGEITDRTYDNPFRNLSPMSALTGAISSEPTIGLYDLKNVIKYAKTDDHI
                     KGIYLNIDEAEGGPANMFDLADALEDFKKSGKFIYAYSKESPELNVMLNNIADKYFVN
                     PVGGIEFDGFGTEMEYYKNLMDKLGVEYKVFYVGEYKSATESFRRTDMSPEDKIQRTE
                     LLKDIYTEYMTRLAKYTGSTFDQLKSIQDNLSIIKPEDIVANKLADGLKYEDEVKNEI
                     KTKLGYKKDEDLNLISYKDYEENNKDKINKSGENKIALVFAEGTIDDSKEDEGTIGGE
                     AYVKMLQKIEQDKDIKALVLRVSSPGGSAFASEQILHQIQNIKKRIPVVVSMGNYAAS
                     GGYYISAYADTIVAEP"
BASE COUNT         3319 a         1953 c         1880 g         3672 t
ORIGIN      
        1 aatagttgct tcaaatatct tcatcaatgt ttcattttat caattatttt attaatacca
       61 tttatattcc cagccattgt tacaattctt tgatattgta atcgccattg taaagcatta
      121 gaaattgtca aataaccttg ttcaggtggt gttttcagga tttgttcagc taactgttgt
      181 agcataattt catcggcagg aatatttttg tcattcaaat atgcaacgat gtcttcaata
      241 ttcgcattgt ccttaaccat ttcacgaagt ctgtaagtgg ttgtatagtc tgcagttctt
      301 tcttttgaaa taaatgaaca actaacaaaa tcaagatttg ctgtttttgt ttttggattg
      361 tcgattttgt cgtaaacgaa tacgagtaaa ttatagccaa gtccaaatat tttttgtttt
      421 gcgtctttaa aagggcaaga ggattgaggt tgtttgattg aagtaacttt tatatcaata
      481 tcaacagaag gtaaatcaat gccttttgcc gaagaaccta tttttagttc atatttttct
      541 gcaagatatt gctgaaattt atgttcaata tgagttccaa cggctttgcc atcagtaact
      601 ccataaagtt cattatttgg tgtctttgat tgagcaatac aaaatgcctt tgcttcaatt
      661 atgacatttt ctattgtcaa attttgtttc atagtgcaaa tttataaaat ttggatttgt
      721 tagttgttaa caatcatttt tttgttctac agtcaaaatt attatgctta ctagtagcaa
      781 tatgtataac aaattaatta aatatactaa tatactacaa tacaacgttt gctacaaagt
      841 tcattctcaa tagaaaaaaa ataccattaa aaaaatgttg ctgggggaat ttgcttcgca
      901 aattccccac attccatttt ataaggataa ttttcaccta caacaatcac aaactcttta
      961 actcctaaat tattacacgc actctaccca tccattctca cgatttttgg ataaaacaat
     1021 ccttccactt gcactaagtc ggtttgcgcc tgcattactt tttgtatgtc tttataggct
     1081 tgcggtgcct catctaatcc tcctccaatt aaagtaacgc cgttttcact caatagtttt
     1141 ttcatctccg acatggaaat tgaagactgc gctttagaac gcgacatttt tcgtcctgca
     1201 ccatgtgaag ccgaatgcaa agatgcctca ttgccttttc cgcttacaat atatcccggt
     1261 gcggtcatag aacccggaat tattcccatt acattttttc cggcaggtgt ggctcctttt
     1321 cggtgtacaa tcacctgctt accgttatgc acttctttcc atgcaaaatt gtgatgattt
     1381 tcaatcacgc taattgcatt ttcattcagt tcttttgcaa ttttatagtg tatggtatga
     1441 tggcaggcag aggcataatc tccggataaa ttcattgctt gccagtactc caatccctct
     1501 tgcgtatcca gcgacaacca tgccaaatga cgagactctt gaggcagttt gcatctttcc
     1561 atcgccagtt tggtgtagtg gttagccacc gtagcaccca gaccacgcga accggaatgc
     1621 gacaacaaac ccagatattt accaatgggt aaatgcatgt tatttgctgc gtcggtaatt
     1681 tctacaatac caaactccac aaaatgatta cccgaacctg aagaaccaag ttgtttagct
     1741 gccttgagtt gtaaagattt cagcatcgga atctcgttga attcaacacg atctaaaata
     1801 gcatgatccg ccgattttac aaactccttc cctgccccga acaaggtatt ttctttcagt
     1861 attttttcaa aataagcttg tttggatttc agcatttttt cgctgacagc aaaaacagaa
     1921 aggctcatgc ggcaaccgat atccacgccc acaccataag gaatgacggc attttctgta
     1981 gctaaaacgc cgccaatagg caggccatag ccctggtgcg catcgggcat aagtgcacct
     2041 gctgctgtaa cgggcagttt catggctgtt tccatttgtt ttaatgcgcc ttcttcaata
     2101 tggttcatcc caaaggatgc ataatccaat gtatcgcttc ggaatgcaaa atgacgggca
     2161 ggctgttcta acctgtcctg aagcaattcg gttgctatcg tcattttatc cgtttcgcct
     2221 tttatcaggc atttcatgtt gtagtctatc agtgcttgtg caattttaga cagtggttca
     2281 ttctccgaaa attcaaacgg gttatccagg atttccttca gcaatatcag tgcgcattct
     2341 aatgtataat gtttaaaatg tttatgcatt acatcaatga caatcgctat agtgcggtct
     2401 tccggatagt tgatttttct aaggtctttg ccttgtaatt ttaattttgc cattttttta
     2461 ttattttttt aagtcaggaa tcaaatgtca agattcaaga atcaagagcc aagagtcaag
     2521 actcgatatt atgaatttat tagacaatat cgacagaaaa aatgaaagtg gtataagttg
     2581 gaatcgaacc aactcctttg gtttttcaaa ccaacgcttc taccaagtta gcttttacac
     2641 cgggaataat ttgataatgt ggaaatgtga aaatgtgaga atgaaataat ttggtaattt
     2701 gatgatttga gtgttctgat ttcatgcatt tactgaatta attaattcgg gttactggtg
     2761 aagaaagtgt ttatattcca agtggaatat cgatacaaat ttttatagaa ttttcgaagt
     2821 aactttcttc tacgacaccg ataaaattag ataatatggt aattcggaaa tgtgatgatg
     2881 tgaaaatgtg aaaatgaaaa aatctcatta tactctaatg caaattacat aaataaaata
     2941 tagcaataag tgagaagtgc tgatttttca ttttgagcga agtatcactt ctcaacggca
     3001 tatattttta aagtggcaat gggtaactgt gttggtaaat cctttttgag tgactgtcaa
     3061 acgatgaaac acaattatac ggcacacatt cttttagttt aagagtttaa gagttaaaca
     3121 atttaatagt ttgggagttt gggagtttaa gagttaaaca gtttaagagt ttgggatttt
     3181 aatagtttta ttgctgattg gatgcaagcg atttgtaatt ctcttttgaa aaaatcgacg
     3241 agtgataatg cagcatgcgt attctttttg ttgttctacg ttgaactatg tcccggggga
     3301 cagcaggact cgaacctgca actcacaagc cattacgaag taacgcatac ttacgacatc
     3361 gtcttatttt ttctttacaa ccaaataatt ttgtactccc ggaaggattc gaacctacga
     3421 ctcccggttt aaaagaccgg tactctgtcc aactgagtta cgagagtata ttgggtgatt
     3481 gttaattggt cattggtcaa tggtgattgg tcattagtaa atgtagatgc caataaatgc
     3541 attatacaat ttaattaaat aaatactggt aaaaagcaat tagtgtttta aaaactgagt
     3601 tatcccttta cgggagttgg atttgaacca acgacctttc atgattagta aaatactcta
     3661 ccgaagtaac acttatttac gacacagcat ttaatttaaa aatgagatga tgtgataatt
     3721 agctaatttg aaaaactgaa atgagttaat tagctaatca gctaattcat aaattcatct
     3781 agtaatttga caattagata aattgatact tttgcggtct atcacggact cgaaccgaga
     3841 agtcttctca tagacagtga gatatgttaa ccaattacac taatagacct gctttgataa
     3901 tttgaaaatt agaaaatgag taaattagct aattcaataa tacatccaat aatttgataa
     3961 ttaaaaagca agtaaccaac taattctgta atagcgttct aaccattgaa ctatatccca
     4021 tcagtgtttt ctatagttgg gatagcagga ctcgaacctg caacctctcg agtagtgatc
     4081 gaagtaacag aattatacga cattgttttt tattaattta ctcccttgta ctgtttgatt
     4141 tataattttt accacataga ggcatagatt tctttaggat taacatctct tgtaatacaa
     4201 aaattgaggt aagaaaagaa aaatatagga atcgccaacg gcgatactct gttattgctt
     4261 gactgtctaa tcagattttc gccaaacagc aaatttatga ttaaaaatgc tatatttcta
     4321 tgtggtttaa actgcacaac gagttaattt actaattcgt taattagcta attagacaat
     4381 taccttttat atcttaaata ttcatatcaa acatttacca tttagcatta aacactaaac
     4441 attactataa gcattttttc tgccgatatg tcaaagaact tttttccgga tgaaaaggat
     4501 tcgggcggta ttacccgaat cctgcatcgc aaactaaact acaccgatgc tcatcttttt
     4561 aatttcactt aatgcatttt caccgttttc gattgctgct aatacatcaa atattttatc
     4621 gctccatcct gcaatcagaa atatgtcatg acgaacaaga tttagtggag tgttaccgta
     4681 tcctgccaaa tcgaacaaat acaatttcgc attcggtgcg actcttttgt atcggttcca
     4741 ggagttctcc atgctacttc cggaatttct gctatcccac agttggcagt cggtaaacat
     4801 catcacttta tctactattt tgttttttgc aatcaaatct tcaatcacca agtaaccgtt
     4861 ggtagagtaa ccgacttcac cttcgcgtcg atagaattca tctacgtttg ccaaaatatt
     4921 tccggttggc atattgatga ttttccatct gtcaccaaac atacccgtga tcacattttt
     4981 gcttttagac tgtaacaaca tcgccaacaa caaaccgata tcatacaaca ttattttgct
     5041 tcgtgccgat accggttttt gcatcgaact ggaaacgtcg caagctaata cgatacgttg
     5101 gtcggcgtca tagcctttga tgttcacggc actcgctttt acagcatctt caattgcacg
     5161 catcacgttt gacgtacatc ctgacggtat agccttcaac tcgcggtatg ccgacaagaa
     5221 acggaaaggc aactgtttgg atttggctac attttcggca ttactcaaat aagcgcaaat
     5281 tttttcgatg ctgttgtacg atacttctgc ttctaatata ttgcgcaagt tgcgcaacat
     5341 cgccatgtaa ccaatttttc cgctggctat catttcttcc catttgttgc cgaatgccgc
     5401 ttttttagct tgttccgttt caaattttgt ttgacctaat acactcaagt tggtttccca
     5461 agtataagga acagtcaaac tgttgcccgc aattttatca aacaagattt gctgcgcttc
     5521 atctttagct tttgggtgta ccaggaacaa agcatcccgc aatttgatgg ctgcatcacg
     5581 gttgtatttc gcgaactgat actcgtcaaa tttattgaac gacattgcca aacctttttg
     5641 aatttgtttg ctcaacttat tcaagcgttt tgttcctgta cgtttgttgg caagctcata
     5701 gtaagccagc aagtcggtaa tttcatcggc acgttgtacc acctggttca ctgtattttt
     5761 cacgatagaa tttccattcg cgcgttttgc cagttctact gccaacacca gtgacgccga
     5821 acgcaagttc attttttgac gggtgtaagc cgcgagctta gccacgaaca gggtgtccac
     5881 ttttgctatc aatgcccgga ttcgttccat acgttcctgt tctttctcgt agaacgtatc
     5941 gctcaatgag gcagtaacaa cggcggcgta caattccaat tcaggcgaca tcgtgtatgc
     6001 atccgctttt tcgtagtttt tcactttagt agtgaaaaca gattttatat tgaatttcat
     6061 cttacttaat ttttttgtta aaccatattt ttttcatcat ttgcttttcg gagttgcata
     6121 gatttattgg gtagccacga ttttttatta caactaccga aaagcatttt atttattttg
     6181 ttgttaattc agcacttgca cacgcttgaa acctaaaact ctgaaagaag tatcttccga
     6241 atctttttcg tgtgtgttaa acaactctgc cagcgacatc ggctctgtta caaatacacc
     6301 gagtttcatt ttatctgaca attgctgtgc tttagctaca ctcagtttcc cgaaatgata
     6361 cttggcaatc agccttcctt ttcttgttaa tgcagtgtct atttttgaaa tcggcatatt
     6421 gaacgtacag atgatttgcg tattcaaaca gtctcccaat aagccatcgg agatattcag
     6481 caaattagaa acggcagagc ttcctgtaat ttgacgatcc atgatgatat tttctgcatc
     6541 ttcgataatc aaaatactgt tgggatattc gagcagcaaa ttcatgaact ccgggttggt
     6601 aatattgccg gccacactgg gcggaacaaa tagcactctt ttcttcacct tgcctatcag
     6661 gtagcgcagg taggttgttt tgcctgtacc cggcaaaccg tgcaacaata cgataccctt
     6721 atccttattt ctgttcagtc gttctcttat tatcttatca gtttctaaaa attcatcttc
     6781 atagtacaag tccaggttta atcttgtttt ttttacatca atacttttca gactaaaatt
     6841 gttgccgttt actgaaatta aattcagttc aaattctttt ttcttttctc tgcgtctgaa
     6901 ttttttaagt tttgatgata aactttcgat ccattcttgt ttgtctcctc ttgtcagaat
     6961 atcacaataa ttgtatgtta tttttagaac cgatttatct ttaagtacga tatttacttc
     7021 atccagttca aatcctttca tattattatt gtcattcaaa ttagaactct ttaaaagtgt
     7081 atcaatttca tccttataat tcgcttcgaa aaacttcaat gccttatcaa cgtcaatagg
     7141 atagatggag tgcatcgtgg gtattactcc atttaatgcc cagaatagcg ctttagcatc
     7201 caaataatca ccaccgttaa agattcggtt gacatctatt gcatatttat tttgtacaaa
     7261 ttccatcttg tatatttttt acttttttga caatactttc acctgacgac ttttgtcgtt
     7321 aatttttaaa ttattccgga ttgataaccg gatttttttg tttaagtcgg gaagttttta
     7381 aaataaaaaa gcccgacttt ttgttggtcg agcttcatat aatgcgagat aaatctttag
     7441 cgtttataaa aagttcgaca cgtcagcgta tcagggtatg ctatcccttt cgccggttta
     7501 aaatgattaa aaatacttct acgcatgtct ttaaaatttt acggtgcaaa tataaactgc
     7561 attttttatg tgtgcaagtt tttttttgaa aaaagttgtc aaaacaacta atttggaatg
     7621 aatataaata aagctaactg atggaatggg gattggggat aggtgattgg tgattggtga
     7681 ttggttattg gttattagtt attagagaat agttattggc atatttgctt ttgttgattg
     7741 cacctacaaa ttatttttga gaaataaatt gtatatttat cgaatattat taatcattca
     7801 aatacaacat tatggaatct gtgaataaca attattctga tttgtcagaa ttagaacaaa
     7861 aaatccaaaa atcattagag agagttcgcg atgaatacac acagttggat gtaaatttaa
     7921 aaaatcaaaa atggataact aacaaaatca aagaatactt aggtgagtta ggttcttctt
     7981 ttggatatga gatttcttcc tcatttcata atcaagaatg gctatatgat gttgtatggt
     8041 ataaaaacaa tgagaacaat atattagaaa aaacaatttt agtggttgaa agtgaactta
     8101 gcaacagaag tataaatagt ttaaaatatg attttgaaaa gttactttta tctaattcgg
     8161 atttgagagt gatgatttgt tttggacaag gaaactttaa ttttcctcag aatgtaaatg
     8221 ggataatcga atcgtttaat aattatgtta caacttatca gaatttaaat tctggtgata
     8281 gaatattgat actagtatgg gatgattata tgaccggaga agtataccca catttaatta
     8341 taaagtaata tacaatatat aatttattat tcaactaatt gcctgctctt caaacagcag
     8401 attctcctgt tctaatttta cgaagttcat gccggttttg tattcctgat caaacagctt
     8461 caacaattgt ctgtacgcaa aaatattact cacccagtcg atgccattta catcaattat
     8521 aacatatttg aaatccggat tttgtttgaa gtattcaaaa tacatgcgct ccagcctgtt
     8581 gagataaaca cgcgaaatat tttgctcgta atctctgccg cgtttgcgaa tgttgcgtac
     8641 cagctggtcg gtttcggcat gtaaataaaa aataatttcg ggcttgggta gttttttata
     8701 aatctgatcg aaaaaatgtt tatataattt atattcttgt tttgtcaaat tgactttggc
     8761 aaacaacaag cattttttga gcatgtaatc cgttaccgtg aaattggaaa aaatattcgg
     8821 ttcgctaaaa atgcgcgtca gctgctgata gcgctctatc aaaaatgaca tctccagttg
     8881 caaggcatat ttggcattat cttcataaaa cttgggtaaa tatgggttgt cttcaaacgc
     8941 ctccaaaatc aattgtccgt gatattcttc tgcaagcaaa gaagctaaag tcgttttgcc
     9001 tgcaccgata ttgccttcta tggtaatgaa tttataaatc attcgggctt aaaaatactg
     9061 acttttgcat catctttaca actattcaag agttcttcta tcgacttacc tgattcggga
     9121 tgtgtataac ttttagcaat ttctgaaagc ggtaacaatg taaaccttcg caaatgcagg
     9181 cgaggatgcg gtatctccaa gtcgctggtt ttgaaaatca aatcattata aaataaaaca
     9241 tcaatatcca gcgtgcgggg tgcgttttgt tttttattct ttcttcccag gtatttttca
     9301 attttttgta gtttgtgcaa aagctgatat ggacttagcc cggtttgaac acagatggcc
     9361 tggtttaagt acttggtttt atgaagcaca ccccaagacg gactttcata tatggcagac
     9421 atcttcgtaa ttgttccaac ttcttctttg ataaaatata aagccaacaa taaattttta
     9481 taccgatgtc ctaaatttga gcccgtgagc agataaactg tattcaagtg taatattttt
     9541 ccaattataa caactatttt tgaactttgt ttcaattaaa tctataaata aaaatcactc
     9601 atgcggaatt tcataaaaat gttctttgcc tgtttattgg caatcgggtt ttctttctta
     9661 ttctttttta tcattttcac catgttggca agccttggtt ctcaaagttc aaaaaaatca
     9721 ggagctaaag ctaatagtgt tttaaatcta acgttaaaag gagaaattac agatagaaca
     9781 tacgacaacc cgtttcgcaa cctttctccc atgagcgcac taaccggtgc tatatcttct
     9841 gagcccacta ttggcttgta tgatttaaag aatgtcatca aatacgcgaa gactgatgac
     9901 catatcaaag gtatttattt gaatattgat gaagcggaag gcggcccagc caacatgttt
     9961 gatttggcag acgcattgga agacttcaag aaatctggga aatttattta tgcttattca
    10021 aaagaatccc cggagcttaa tgtcatgctt aataatattg ctgataaata cttcgtgaat
    10081 ccggttggag gaatagagtt tgacggcttc ggaacagaaa tggaatatta caaaaacctg
    10141 atggataagt taggtgtgga atataaagtg ttttacgtgg gcgagtacaa aagtgcaaca
    10201 gaaagtttca gacgcacaga catgagtccg gaagataaaa tacagcgtac tgaattactt
    10261 aaagatatat acacggaata catgacaaga ttggctaaat atacaggctc tacttttgac
    10321 caacttaaat caatacaaga taatttatcc atcatcaaac ctgaagatat tgtagctaac
    10381 aaactggctg acggtttaaa atacgaagat gaagtaaaaa atgaaatcaa aactaagctg
    10441 ggttataaaa aagacgaaga tctgaatttg ataagttata aagattacga agaaaacaat
    10501 aaagataaaa taaacaaatc aggcgaaaat aaaattgctt tagtatttgc tgaaggaacg
    10561 attgatgaca gtaaggaaga cgaaggcacg ataggtggcg aagcatatgt aaaaatgctg
    10621 caaaaaatag aacaggataa agatatcaaa gcattagtat tgcgtgtaag ttcaccgggt
    10681 ggcagtgcgt ttgcatcaga gcaaatttta catcaaattc aaaatataaa aaaacgcatt
    10741 ccggttgtag tttccatggg caattatgca gcttcgggcg gttattatat ttctgcttat
    10801 gcggatacga ttgtagcaga accc
//
LOCUS       DAOQII010000061        10815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3135_length_10815_cov_4.867472, whole genome shotgun sequence.
ACCESSION   DAOQII010000061 DAOQII010000000
VERSION     DAOQII010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3135_length_10815_cov_4.867472"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PLF48_04470"
     CDS             complement(<1..179)
                     /locus_tag="PLF48_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNF48610.1"
                     /translation="MTKRKKIALVGNPNSGKSSLFNQLTGLNQKTGNFPGVTVERKNG
                     ISKYDEQTEWEIIDLP"
     gene            complement(179..427)
                     /locus_tag="PLF48_04475"
     CDS             complement(179..427)
                     /locus_tag="PLF48_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNF48611.1"
                     /translation="MNNLPAITDLFSGETAIVSEYANCFIACKLTAMGLLPGTEITLV
                     RKSLFGTTLYLKTESHALCLRKEEAQDILITRLSRETA"
     gene            complement(611..2749)
                     /locus_tag="PLF48_04480"
     CDS             complement(611..2749)
                     /locus_tag="PLF48_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNF48612.1"
                     /translation="MAKYDSNHVKNVALLGHAGCGKTTLAESMLFEAGVINRMGTIAG
                     KNTVSDYHELETERESSVFASLLHTPWKDYKINIIDTPGYDDFAGEVISALRVADTGV
                     IVINAANGVEVGTDIIWEYTEKFKTPTLFVFNQLDKDEADFDKTLQEAKSHFGSNVVA
                     VQYPVQTGANFNSIIDVLNMVMYVYPPEGGKPEKQDIPANEKERASKLHQELVEAIAA
                     NDEGLMEKYFEQGELSEDEMKNGLHTSMIKHDIFPVFCCAAAKNMGSGRIMSYIDNVC
                     PSANEMPPQKTKAGTELKCDPNGPACIFIFKTIIEPHIGELSLFKVFSGTIRSGIELV
                     NENTGVTEKINQLLLLEGHNRIPVDELTAGDIGATIKLRSTHVNNTLHAKGANLELAP
                     IVFPSTNVTVAIAAVNKNDEEKLALALHTLKEEDATVHVEVSPELKQTLLHCMGELHL
                     KVIEWKINHIHKLDVVFDKPKIPYRETIQASAEANFRHKKQSGGAGQFGEVHLRVEPW
                     YEGMPAPLHLNLRHEEVINLEWGGKLVFCNCIVGGAIDARFLPSILKGVMEKMQNGPL
                     TGNYVRDVRVSVYDGKMHAVDSNDISFKIAGSQAFRQAFFEAQPQILEPIQKVEVLCP
                     DEMVGAVIGDLQSRVGIVEGMEGEGHFQKITAKVPLSHLYQYSSTLRGLTQGRARFTM
                     QFSDYQPVSYDVQKKLIEAHSHHSDFENED"
     gene            complement(2804..3160)
                     /locus_tag="PLF48_04485"
     CDS             complement(2804..3160)
                     /locus_tag="PLF48_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48613.1"
                     /translation="MTTEAEHKALLDDCKSWYETLAAYREKINVMKNELYFFAPGKTD
                     AEVKVGIEHFHNQFHIQLINVHDLKHEIRYHLHQAEAHPDLSYQVPHEDLKEKVQFMT
                     GYLDKLSDEFHAFIKQ"
     gene            3311..4051
                     /locus_tag="PLF48_04490"
     CDS             3311..4051
                     /locus_tag="PLF48_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF48614.1"
                     /translation="MVILKFFYSIWIVVSFLLLTLPMLIGYLFVKLLPYKSQIKGVYA
                     VNRSFLFIWSVFSGLRYRINGLENIDKNQTYVVVVNHVNAADLIAVAYGQRVPAKPLV
                     KKELTLIPGLGQLFSLACLPLDRSSKQARYESKQKMLADLKQGISVLIFPEGTRNRSN
                     DPLLPFHDGAFEIAIDADVPVLPVVLTNIRKINRVDTLLVQPGILELTHLPPIKASNS
                     GLDVVSLKKVVFDNMKNYIESQDDFFNK"
     gene            complement(4041..5069)
                     /locus_tag="PLF48_04495"
     CDS             complement(4041..5069)
                     /locus_tag="PLF48_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF48615.1"
                     /translation="MKPLINFLILLLCSVFAFSQDLHYSQFYSIPSNANPAFTGFFNN
                     DYFVAGIYKTQWNTVSKPYQTVGATMELSLLKNKFPESYLGTGVEVVHDWAGSTIYTT
                     DIFNFNLSYLHVLDKESRNVLGFGFQNGLVYRKFDLSKATFENQFNGYNAFDPTKDPL
                     ESNLKTKQIDYNLSLGTLYSFSPNANYNFYLGFSAFNVTQPNISFFNGSEEKLERRYS
                     IQTGAKIKLKNTWSLLPSAMFQLQGKNLEALFGSFVRYGYVENRKEKFGLSVGAWYRF
                     KDALIPAIKAEYKNIALTMNFDINVSKLTPASRFFGSGEISVSYSGLLFKEHVKPKKP
                     IYCPAFIY"
     gene            complement(5275..9897)
                     /locus_tag="PLF48_04500"
     CDS             complement(5275..9897)
                     /locus_tag="PLF48_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF48616.1"
                     /translation="MFFILPLQADIGIGSRCITILVKRLLIIGSIFLSLPVMAQLNAD
                     FTLSKDKGCSPLLVNFTNTSSGSPDSCFWSLGINGNTSDNCNPSAIYNIPGVYQVTLT
                     VFKGNLRSSKTKTVTVFHDPVAQFNVSPATGCIPFSVQFQNMSTSGDAPITSVLWDMG
                     DGRTNSSQNPNYTYTAGGSLTVSLIITDANGCKNTITKPNIIKANIPPVVDFTIATNE
                     SCRLPFTTNFTSVVTPNTNITYAWNFGNGLTSSTANPTATYVGGGNFPVSLTVTDENS
                     CHTTKTISDAVRVNPFKINVDLQATICENTTMHPTVNTTFQNVHYFWNFGNGTNSEEQ
                     SPEIIYHTPGNYTLTCLAVTDDGCRDSVRLPVHVVENAEADFTSSLTFSCSPFTLSLV
                     NQSTNAVNYQWSIWGPGGFFTSSTVANPSIFLPSIGLYTIELRAYSANGCISYIKKED
                     FAGVRPNTLSPHADKKYGCKPLTVEFSANPNVSASSYLWDFGDGTTGTGERVQHTYIQ
                     EGDYLAKVTAVYPPPCVTVTVPVDSIHVGFRTPFSGDFDLTSVCVHKETVTYQAHGGT
                     PNTIFTWMFGDGEGQGAMATHVYQDPSDPDKYTVYLVAQNGGCRDTIPIKQIFVAYPK
                     ADFTINTVCGSNTVTLNNTSRGFTTAIWSFGDGTTLTTNDPSVTHTYASNQQVLVKLK
                     VYNATTGCWDSIGRLIDFVSDINLNYTSENTSGCKPLIAHFLAPYDTMIQTYTWDFGN
                     GIRGYGRNIYGIFPNEGTYYVTLEMKLKNGCTVRSTRIDTVQVYDVHPNFTMQHSGNC
                     LPANYTFKDSSIANSSGIKSIKWLIDSTINLSGNQVNYTFTTSGIHTVKIIVENNNGC
                     VRSLTKTITIDAPYVDFSADKDFVCAGTTIHFTNLSSGKIQSYLWDFGDGTTSSDASP
                     DHHFSQERNYTIILTVTDSSGCTVSISKNQYVHIKNLHVDFTASPRFKSCPDLISNFQ
                     VLNNTPTTYQSISWDFGNGNSSNNLNTTPQAVYTRADSFDIRLIVTDVNNCTDTVFKK
                     DYIIVSGPSGEFTFTPDSGCLPLNVGFNASFKNTTTTIWDFGNGDTRTDNTASNRLNY
                     NYTREGDYTPAVVLKDNFGCTVNVVSTHKVRVGRLSAQFNLNKNSLCLGSDQVVLSDS
                     VYTSQNTAITQKYWTVNDGVTIDTGVGNTYLPTSNRPLEFKFKVANTFGCEQSITKNL
                     QVYEYPITVATEDKIICKGESVPINIQTNATHTSWTPATGINNPNATLVVASPIVNTE
                     YIIKTYQNALCPIYDTLSILVKTAINAQAFPDTVICYGDSVQLHGIADNTSLYQTKVF
                     WTPASTLTSQTDFNPIAFPKHATTYLATFTNGTCAAKQIPVFVDVKPLPTVTAGEEQI
                     IIKGMSIDLTSVSSNATSYSWFPDYNIACTNCQNTQAKPEVDTTYFVEVKNQYGCKAL
                     ANQKVKVIEDCAGNMVFLPNTFTPNGDGQNDLLHVMGPGISSVKVFRIFNRWGELVFE
                     TRDMNEGWDGTYKGEKLNPAVFVYYLEVECIDFRTTMKKGDITLLR"
     gene            9961..10674
                     /locus_tag="PLF48_04505"
     CDS             9961..10674
                     /locus_tag="PLF48_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNF48617.1"
                     /translation="MKKNIGIGNLSVVPMRNEASDRAEMVNQLLYGETFDIIERKEKW
                     IKIQLHHDNYMGWIDNKQYALKTEFEAHDCIVSNLFQQNHIKKQTYWLPMGALVEKKY
                     LQKCTYNLSVLNAAKQFLESPYLWGGRTFMGIDCSGLTQVVYRINGILLNRDAHEQAK
                     QGEAVAFDEARNEDLAFFSNDEKKIKHVGIVFKEDKQLKIIHASGKVRIDTLDEKGIY
                     NATTQQYTHQFSHLKRFTI"
     gene            10671..>10815
                     /locus_tag="PLF48_04510"
     CDS             10671..>10815
                     /locus_tag="PLF48_04510"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNF48618.1"
                     /translation="MKNIAVLTGAGISAESGIKTFRDSDGLWENYRVEEVASFDAWQK
                     NKEL"
BASE COUNT         3334 a         1959 c         2030 g         3492 t
ORIGIN      
        1 ggtaaatcta taatttccca ttctgtttgt tcatcatatt tagagatacc atttttacgc
       61 tctaccgtaa ctccgggaaa atttcctgtt ttttggttta atccggtaag ttgattaaat
      121 aaagacgatt ttccggaatt gggattgccg actaatgcta ttttttttcg ctttgtcatt
      181 atgcggtttc cctgcttaaa cgggtgatta atatatcttg agcttcttct tttcgcaggc
      241 aaagcgcatg gctttctgtt ttcagataca gtgtagttcc aaataaggat ttccttacta
      301 aggttatctc agttccgggc aacaagccca tagccgtcag tttgcatgca ataaaacaat
      361 tcgcatattc actgacaatt gcagtctctc cggaaaataa atcagttata gcaggtaaat
      421 tgttcaagat tagaattatt ctaaataaca aaagtacgtt taattttgct taattaaagt
      481 acatcaccaa aattacctaa catttggtaa atgtagttta gttggtacaa aatagagttt
      541 attcataaaa aaaacctctg ccattatttc ggacagaggt ttttaaatac tgattatgat
      601 ttaatttgtc ttaatcttca ttttcaaaat cagaatgatg cgagtgggcc tctatcagct
      661 ttttctgtac atcataagat acaggctgat agtcagaaaa ctgcatggtg aaacgtgcac
      721 gtccttgtgt taatcctcgt aaggtagaag aatactgata taaatgagaa agcggtactt
      781 tggctgttat tttctggaaa tgaccttctc cttccatccc ttctacaatt cctactcttg
      841 attgtaaatc gcctataacg gcacctacca tttcatcagg gcaaagtact tctacttttt
      901 gtataggttc taaaatctga ggttgtgcct caaaaaatgc ctgacgaaat gcctgtgaac
      961 ctgcaatttt aaatgaaata tcattactgt caacagcatg cattttaccg tcataaacag
     1021 aaacacgtac atcgcgaacg tagtttccgg taagcggacc gttttgcatt ttttccatca
     1081 ctccttttag aatggatggc agaaaacgtg catcaatagc accaccaact atacaattac
     1141 aaaataccag tttgccgccc cattccaaat ttattacttc ttcatgtcga agatttaaat
     1201 gaagtggtgc cggcatgcct tcataccaag gctcaacacg taagtgcact tcgccaaact
     1261 gacctgcacc gccactttgt tttttatgtc tgaaatttgc ttctgctgaa gcttgtatgg
     1321 tttcgcgata aggaattttt ggtttatcaa aaacaacatc cagtttatgg atatgattga
     1381 ttttccattc tatcactttc aagtgcaatt cgcccataca gtgtaacaag gtctgtttta
     1441 attcaggcga aacttccacg tgtactgtag catcttcctc tttaagtgta tgcagcgcaa
     1501 gtgcaagttt ttcttcatcg tttttattaa ctgcggcaat ggctactgtt acatttgtac
     1561 ttgggaatac aatgggagcc agttctaaat ttgcgccttt tgcatgcaag gtattattta
     1621 catgggtaga gcgaagctta atagtagctc caatatcacc tgcagtcaat tcatctacag
     1681 gaatgcggtt atgtccctca agcaagagga gttgatttat cttttctgta acaccagtgt
     1741 tctcattgac taattcaatg cccgaacgga ttgtgccact gaatacttta aataagctta
     1801 attctccgat atgcggttct ataatggttt taaaaatgaa aatacatgcc gggccattcg
     1861 ggtcgcattt taattcagtg ccggcttttg ttttttgcgg aggcatttcg tttgctgatg
     1921 gacaaacatt gtcaatataa ctcatgatac gaccactgcc catatttttt gcggcagcac
     1981 aacagaatac agggaaaata tcgtgtttta tcatggaagt atgcaaaccg tttttcatct
     2041 catcttcgct cagttcacct tgttcgaagt acttttccat cagtccttcg tcattggcag
     2101 caatcgcttc taccagctcc tggtgcaatt ttgatgcgcg ttccttttca tttgccggaa
     2161 tgtcttgttt ttcaggtttt cctccttccg gcggataaac gtacatcacc atattcaaca
     2221 catcaatgat gctgttgaaa tttgcacctg tttgtacggg atactgaacg gcaacgacat
     2281 tactgccaaa atgagatttg gcttcctgca aggttttatc aaaatcagct tcatctttat
     2341 caagctggtt gaaaacaaat agagtaggag ttttaaattt ttcggtatac tcccaaataa
     2401 tatctgttcc tacttctact ccgttagcgg cattgataac aattacgccg gtatcggcta
     2461 cacgcaatgc actaatcact tctcctgcga aatcatcata tcccggcgta tcaatgatat
     2521 taattttata atctttccaa ggggtgtgca gcaaagatgc aaaaacagaa ctttctcgtt
     2581 cagtttccag ttcatgatag tcagatacgg tattttttcc ggctatcgtt cccatccggt
     2641 tgattactcc ggcttcaaaa agcatacttt cagccaaagt ggttttgcca caccctgcat
     2701 gccctaatag tgccacgttt tttacatggt tagaatcata ttttgccata atggtgtctt
     2761 tagaatttaa aaatgaaaaa agtcggcata cgccgactaa attttattgt ttgataaatg
     2821 catgaaattc atcacttaat ttatccaagt atccggtcat gaactgtact ttttctttca
     2881 agtcttcatg aggtacctga tagcttaaat ctggatgagc ttcagcctga tgcaaatgat
     2941 aacggatttc gtgttttaaa tcgtgaacat taatcagctg gatatgaaat tgattgtgga
     3001 aatgttcgat acccactttt acttcagcgt cagttttacc gggagcaaaa aaatacaact
     3061 catttttcat tacatttatt ttttcgcggt aggctgctaa agtttcatac cagcttttgc
     3121 aatcatcgag gagtgctttg tgttctgctt ctgttgtcat gttgattgaa tttattatag
     3181 attagtaaaa ttgaatgttt taattaagct gttaaatttc cgattaattt ttaaaatttg
     3241 aaatgataaa aatcatactt gatgtgcatc taaaaatagt attttttatt tttattgtgc
     3301 aatacacatt atggtcattt tgaaattctt ctacagcatc tggatagtgg tcagtttttt
     3361 actgcttacc ttacccatgc ttatcggcta tttgtttgta aaactgctac cttacaaaag
     3421 tcagataaaa ggcgtctatg ccgtcaatcg ttcttttctg tttatatggt ctgttttttc
     3481 tggtttgagg tacaggataa atgggcttga aaatatagat aaaaaccaga cttatgtagt
     3541 ggtcgtcaat catgtaaatg ctgctgattt gatagctgta gcttatggac agcgtgttcc
     3601 ggcaaaacct ttggtgaaaa aagagcttac attaattcca ggacttggtc aattgtttag
     3661 tttggcatgt ctgcccttag accgaagcag caaacaagct cgctatgaaa gcaaacaaaa
     3721 aatgctggct gatttaaaac aaggtatttc tgttttgatt tttcctgagg gtacgcgcaa
     3781 cagaagtaat gatccgttac tcccatttca cgatggggca ttcgaaattg ctattgatgc
     3841 tgatgtgccg gttttgcctg tagtgctaac taatatcaga aaaataaacc gggttgacac
     3901 gcttctcgtg caacccggaa ttttggaact tacccacctt ccccctataa aagcaagtaa
     3961 ttccggtttg gatgtagtta gtctgaaaaa agtagttttt gataatatga agaattatat
     4021 tgaatcacag gatgactttt ttaataaata aatgccggac aataaattgg ttttttaggc
     4081 tttacatgtt ccttaaataa taatccggaa taggaaactg aaatttcacc gctgccgaaa
     4141 aatctgctcg caggcgtcag cttggatacg ttaatatcaa aattcatggt aagtgcaata
     4201 tttttgtact ccgcttttat agccgggatc aaagcatctt taaaacgata ccaagcacca
     4261 acactcaatc cgaatttttc ttttctgttt tcgacatagc catatcgtac aaaacttcca
     4321 aataaagctt ccagattttt tccttgtagc tgaaacatag ctgatggcaa taatgaccaa
     4381 gtgtttttta atttaatttt tgcaccagtc tggatactgt atcttctttc caacttttct
     4441 tcactgccat taaaaaatga aatattgggt tgtgttacat taaatgcaga aaaccctaaa
     4501 taaaaattat aattggcatt tggcgaaaat gaatatagcg tgcctaaact gagattataa
     4561 tctatttgtt tggtttttaa gtttgattct aaaggatctt tagtcggatc gaaagcattg
     4621 tagccgttaa actgattttc aaatgtagct ttagacaagt caaattttcg gtaaaccaat
     4681 ccattctgaa atccaaaacc caacacattt cggctttctt tatccagcac atgcaagtaa
     4741 gacagattga aattgaaaat atcggtggta taaatagtag aaccggccca gtcatgaacg
     4801 acctcgacgc cggttcctaa ataagattcc gggaatttgt tttttaacaa cgacagttcc
     4861 atcgtagcac ctacagtctg gtatggtttt gaaactgtat tccattgggt tttataaatt
     4921 ccggcaacaa aataatcatt gttgaaaaat ccggtaaatg caggattcgc attggatggt
     4981 atggaataaa attgcgaata atgcaaatct tgcgaaaatg caaaaacact gcataacagt
     5041 aaaattaaaa aattgattag cggtttcatg tttcttttat ttagggggtt tatatttgta
     5101 gtaagtaata agtattaagt aggcagtttt tttataatcc aaaatttata atttaaaatt
     5161 caacattaag cactcaatat tcaacattag aaagtaattc gtaattagta gcgttttttt
     5221 tgtaattcaa aatttataat ttataattta aaattaatca ttcatcattc aacattaacg
     5281 aagcagagtt atatctcctt ttttcatagt ggtgcggaag tctatacatt ctacttctaa
     5341 ataataaaca aacacggcag gatttagttt ttcaccttta taagtgccat cccagccttc
     5401 gttcatatct ctggtttcaa atacgagttc tccccagcga ttaaaaatcc ggaagacttt
     5461 tactgatgaa ataccgggcc ccataacatg cagcaaatca ttttgaccat caccatttgg
     5521 cgtaaatgtg tttggcagaa agaccatatt gcctgcacag tcttcaatca cttttacttt
     5581 ttgattggct agcgctttac agccgtattg gttttttact tcaacaaaat aagtagtatc
     5641 cacctccggt tttgcctgtg tattttgaca gtttgtgcaa gcaatattat aatcaggaaa
     5701 ccaagagtaa ctagtggcat tactcgaaac cgaagtaagg tcaatactca ttcctttgat
     5761 gatgatttgt tcctcacctg ctgtcacggt tggcaaaggt ttgacatcta caaatacagg
     5821 gatttgtttg gcagcacaag tgccattggt gaatgtagcc agatatgttg tggcatgttt
     5881 tggaaatgca ataggattaa aatcagtttg agaagttaaa gtgctagcag gtgtccaaaa
     5941 cacttttgtc tgatataatg aagtattatc agcgatgcca tgcagttgaa cagagtcgcc
     6001 ataacaaata acagtatcag gaaacgcttg ggcattgatt gctgttttta ctagaataga
     6061 taatgtatca taaattggac ataaggcatt ttgataagtt tttatgatat attctgtatt
     6121 gacaatgggt gatgccacta caagtgtagc atttggatta ttgatgcctg ttgctggtgt
     6181 ccatgatgta tgagttgcat ttgtctgaat atttatcggc acactttcac ctttacaaat
     6241 aattttatct tcagtagcta ccgttatagg atattcatat acctgcaagt ttttcgtaat
     6301 ggactgctca caaccaaaag tattggccac tttaaatttg aactccagag ggcggttgct
     6361 ggtaggcaga taagtatttc caactcctgt atctatcgta acgccatcat ttactgtcca
     6421 gtacttttga gtaattgctg tattttgaga tgtataaaca gaatcggaaa gcaccacttg
     6481 atctgaaccc aggcaaagtg aatttttgtt taaattaaat tgagctgaaa gacgacccac
     6541 acgaacttta tgtgtcgaaa ctacatttac ggtacaacca aaattatctt ttaatacaac
     6601 tgccggtgta taatcacctt cacgagtata gttgtaattt aatctgtttg atgccgtatt
     6661 gtctgttcgc gtatcaccat tgccaaagtc ccaaattgta gtagtggtat ttttgaatga
     6721 tgcattaaat cctacattta acggaagaca accggaatcc ggggtgaaag taaattcacc
     6781 gctagggccc gaaacaataa tataatcttt tttgaaaaca gtatctgtac aattgtttac
     6841 atcggtaacg ataagccgaa tatcaaaaga atctgcacgg gtataaactg cttgtggtgt
     6901 tgtatttaaa ttattactgg aattgccatt tccaaaatcc catgaaatcg attgataagt
     6961 agtaggggtg ttgtttaata cctgaaaatt ggaaatcaaa tcaggacaac ttttaaatct
     7021 cggcgaagcc gtaaagtcaa catgcaaatt tttaatatgc acgtattgat ttttactgat
     7081 agaaacagtg cagccactgg aatcggtaac ggtgagtata attgtgtaat ttctttcttg
     7141 tgagaaatga tgatcgggag atgcatcaga ggatgttgtt ccatcaccaa aatcccataa
     7201 ataggattgt atttttccgg atgaaagatt tgtaaaatgg atagtagtgc ccgcacaaac
     7261 aaaatcttta tcagctgaaa aatcaacata cggtgcatct attgtgattg tttttgttaa
     7321 actgcgtaca cagccgttgt tattttctac aataattttt actgtatgta ttccggatgt
     7381 cgtgaatgta taattgacct gattgccact caaatttatg gtactgtcaa tcagccattt
     7441 tatacttttt attccagatg aatttgctat acttgaatct ttaaaagtat aattagccgg
     7501 caagcaattg ccggagtgct gcatggtaaa attaggatgc acatcataaa cctgaacggt
     7561 atcaattcgt gtagaacgca cggtacaccc attttttaat ttcatctcta aggttacata
     7621 ataagtgcct tcattaggga aaataccata aatatttcgc ccgtaaccac gtatgccatt
     7681 tccgaaatcc caggtatatg tttgaatcat ggtgtcatat ggtgctaaaa aatgtgcaat
     7741 caaaggtttg catcctgatg tgttttctga agtataattt agattgatgt cggaaacaaa
     7801 atcaattaat ctgcctattg aatcccagca accggttgta gcattataaa ctttaagctt
     7861 aaccaatact tgttggttgc ttgcataagt atgtgttact gacggatcat tggtagttaa
     7921 tgtcgtgcca tcaccaaaac tccagattgc cgtggtaaat ccccgggaag tattattaag
     7981 cgttaccgta ttactgccac ataccgtatt gattgtaaaa tcagcttttg gataagcaac
     8041 aaatatttgc ttgattggaa tagtatctct gcaaccacca ttttgtgcta ctaaatagac
     8101 cgtgtattta tccggatcgg atgggtcttg atatacatgt gtagccattg ctccttgtcc
     8161 ttcaccatca ccaaacatcc aggtaaatat ggtattaggt gtaccaccgt gtgcttgata
     8221 agtaacggtt tctttgtgta cacaaacact tgtcaaatca aaatcacctg aaaatggtgt
     8281 cctgaaacca acatgaatag aatctacagg tacggtaaca gttacacaag gaggagggta
     8341 aacagcagta acttttgcta aataatcacc ttcttgtatg taagtatgtt gtactcgctc
     8401 acctgttccc gtagtgccat caccgaaatc ccataaataa gaactagctg aaacattagg
     8461 atttgctgaa aattccactg tcaagggttt gcatccatat tttttatcag catgcggaga
     8521 gagtgtattt gggcgaacac cggcaaaatc ttcttttttt atataactta tacatccgtt
     8581 tgccgaataa gcacgcagct caatcgtata taatccaata cttggtaaaa atattgacgg
     8641 attggcaacc gttgaagagg taaaaaatcc accggggccc caaatactcc attgatagtt
     8701 gacagcatta gtggactggt tgactaaact tagtgtaaaa ggtgaacagg aaaaagttag
     8761 agatgaagta aagtcagctt ccgcattttc gacaacatgt acgggtaatc gaacggaatc
     8821 gcgacaaccg tcatcagtta ctgccagaca agttaaagta tagttacccg gtgtatgata
     8881 tattatttcc ggtgactgct cttctgagtt tgttccattt ccaaaattcc aaaaataatg
     8941 tacattctgg aaggtggtgt taactgtagg atgcattgtg gtattttcgc atattgtcgc
     9001 ttgcaaatca acattgattt tgaaaggatt tacccttaca gcatctgata tcgtttttgt
     9061 agtatggcaa gaattttcgt ccgtaactgt aagcgatacc ggaaaatttc caccacccac
     9121 ataagttgct gttggatttg ctgtggatga agtgagtcca ttgccaaaat tccaggcata
     9181 ggtaatattt gtattgggag taactacgct tgtgaaatta gtagtaaagg gcaaacggca
     9241 agattcgtta gttgcaatgg taaaatcaac taccggaggg atattagcct taataatatt
     9301 cggttttgta attgtgtttt tacagccatt tgcatctgta attatcaatg aaacggttaa
     9361 actgcctccg gcggtataag tataatttgg attttgtgac gaatttgttc tgccgtcacc
     9421 catatcccat aaaacggaag taatgggtgc atctccggat gttgacatgt tttgaaactg
     9481 cacggaaaaa gggatgcaac ccgtggcagg gctaacatta aattgtgcaa cggggtcatg
     9541 aaatactgtt actgttttag ttttggatga acgcaaattt cccttgaata cggtgagtgt
     9601 aacctgataa acacctggga tattatatat ggcacttgga ttgcagttgt cagaagtgtt
     9661 gccattgata cctaaactcc aaaaacaact gtcaggactt ccggacgaag tattcgtaaa
     9721 gtttaccaat aatggcgaac aacccttatc tttgctcagg gtaaagtcag cgtttagttg
     9781 tgccataaca ggtaacgata aaaatatcga acctattatc aataaacgct taactaatat
     9841 tgtaatacat cttgagccaa taccaatatc ggcttgcagg ggtaatatga aaaacatgat
     9901 acatgggttt ttatgttaaa acaatattaa acctaatttt tggaaatgtc aatagaaagt
     9961 atgaaaaaaa atatcggaat cggaaattta agcgtagtac ctatgcgaaa tgaagcgtca
    10021 gacagggccg aaatggtcaa tcaactgctt tatggcgaaa catttgatat catagaacgc
    10081 aaagagaaat ggattaaaat ccaattgcac catgataatt acatgggttg gattgataat
    10141 aaacaatacg cattaaaaac agaatttgaa gctcacgatt gtatcgtaag caatttgttt
    10201 caacaaaatc acattaaaaa acaaacttac tggctaccta tgggagcgct tgttgaaaaa
    10261 aaatatttgc aaaaatgcac ttataattta tcggtattaa atgcggcaaa acaatttttg
    10321 gaatctcctt acttgtgggg cggaagaaca tttatgggaa tagattgttc cggattgaca
    10381 caagttgtct atcgcataaa tggaatcttg ttaaacagag atgcacacga acaagccaaa
    10441 caaggcgaag cagtagcatt tgatgaagcc agaaatgaag accttgcctt ttttagtaat
    10501 gatgaaaaaa agataaagca tgttggtata gtttttaaag aagataaaca actcaaaata
    10561 attcatgcca gcggaaaagt acgtattgat acgctggatg aaaaaggtat ttataatgcg
    10621 actactcagc aatacaccca tcagttttct cacttaaaga gatttacaat atgaaaaata
    10681 ttgcagtatt gacaggagcc ggaataagtg cggaaagtgg cattaaaact tttcgagata
    10741 gtgatggttt atgggaaaac tatagagtag aagaagtggc ctcttttgat gcatggcaaa
    10801 aaaataaaga actgg
//
LOCUS       DAOQII010000062        10722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3175_length_10722_cov_4.451580, whole genome shotgun sequence.
ACCESSION   DAOQII010000062 DAOQII010000000
VERSION     DAOQII010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10722
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_10722_cov_4.451580"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2016)
                     /locus_tag="PLF48_04515"
     CDS             complement(<1..2016)
                     /locus_tag="PLF48_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNF48619.1"
                     /translation="MKYLLSLLFLVLFFIWNTDAQTYYKHYDVEVVKNSSLLPYPWMG
                     GLNNAQFANADVNHDGKKDIVVYDNYNRQFFVFLLKTVGSTAYTFAPQYAAQFPDISN
                     WMILKDYNCDAIPDLFTYNGLSNTKVFKGYYDQDTLKFKLQQNGFFYNNGITNVYAVD
                     VSRPIIEDINKDGDLDFISFETFGTYLYYYENQRVEKSLPCDTFTFNLVDHCWGNVRD
                     TFSATYSIRDTCMGKFRLGSPDQILHTGSFMESADLDNNGKVDLLIGNIGIQNITALY
                     NMGSFSYASILKQDARYPSYNTSFSATSMATPMKLDADGDGRNDLIVSTFDVGSNNID
                     NIYWYKNLSADSFNLSLQTKNFLLTDIIDNGENSAPCIFDYNGDGLKDILIGNGGYKD
                     NIHPIKYTLELYKNIGSDTLPKFELVNADYLDVSTFGIRDLIPNAGDIDNDGDTDIIA
                     GFNTGNILWWENTAGNGNIPVFVFRGLLKDNAATDILVEQQAAPFIADVNGDGNNDLL
                     IGDKNGRISYYSGSGNNSINLALVTSNFGKIRTNSLYNNFGYAQPTVADINHDGLQDL
                     VIGSNSIGINWYNNYSSHQPNDSLIPTGMLLGNKIFARSTTAIADLDSDNKLEILVGN
                     YNGGLWYYSEKIPIVTAVNSNPTQTLPFNIFPNPAGNFITLEFDKSLL"
     gene            complement(2080..2742)
                     /locus_tag="PLF48_04520"
     CDS             complement(2080..2742)
                     /locus_tag="PLF48_04520"
                     /inference="COORDINATES: protein motif:HMM:NF007458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HNF48620.1"
                     /translation="MKYNSVIVIVVCLLLFENCKHHTSNAINSGKMPLVELNSKSDIF
                     QEGDILFHTSTSSQSRAIQSATHSVYSHCGLLFHRNANDKNWYVLEAVQPVKWTPLDK
                     WIARGKAKHAVIKRAAITINQQDAIKNEAMKFLGKNYDYTFEWSDNKIYCSELVWKAY
                     FNATGIKLGSLQPLSSLDLKNKQVSKKLKERYGNKIPYTEKVISPNAIFTSDKLTTVY
                     SQ"
     gene            complement(2886..3860)
                     /locus_tag="PLF48_04525"
     CDS             complement(2886..3860)
                     /locus_tag="PLF48_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNF48621.1"
                     /translation="MSVLFFIETANGKVKKNSFEAASYAAQVASKLGTDAVGIALGTA
                     NDVELANLGIYGANKVLHASNSAFDNFDSGVYAKAIADAADKAGADVVVMSMSLTGKA
                     VAPRVAVRLDAGIVSGVVSLPDMTNGFVVKRGAFSGKAFAFVNINTPKKVVSVTPNAF
                     GAVKGEGTATVEAVDLTPAASRITVKEISRQTGDIAPLPEAELVVSAGRGMKGPENWG
                     IIEDLAKVLGAATACSRPVADVHWRPHYEHVGQTGVAIRPNLYIAIGISGAIQHLAGV
                     NQSKCIVVINKDPEAPFFKAADYGIVGDAFEVVPKLTEAIKAFKASQH"
     gene            complement(3862..4605)
                     /locus_tag="PLF48_04530"
     CDS             complement(3862..4605)
                     /locus_tag="PLF48_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNF48622.1"
                     /translation="MKFLVCISLVPDTTTKISFVDNDTKFNNDKVQWILNPYDEWYAL
                     VRALELKEANGGTVTVVNVGPAENDAVIRKALAIGADDAIRIDAAGELDAFAIASDIA
                     DVAKSGAYDMILLGKETISNNGSNLGGMIAELMDLPFISYASKLEMSGNTATIEREIE
                     GGKEILSVDVPFVLSAAKGMAEARIPNMKGIMAAKSKPLQVVTPKGVASLTTTVKYEL
                     PPAKAACKMISPDHVDELVQLLHNEAKVI"
     gene            4658..4981
                     /locus_tag="PLF48_04535"
     CDS             4658..4981
                     /locus_tag="PLF48_04535"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF48623.1"
                     /translation="MHSIRLEKLLALYEENPTDSFINFALAKEYELNNNLNKAIQLLE
                     KLIITNPNYLGTYLHLAKLYEKNGEIKKALNIYEKGLIVAKHAKDLHSLSELKNAKMN
                     LELEE"
     gene            5029..5475
                     /locus_tag="PLF48_04540"
     CDS             5029..5475
                     /locus_tag="PLF48_04540"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNF48624.1"
                     /translation="MSDLQKTIQMVENSIRKLGIDPSTCKGEKEGQYSLNRGSAAVWI
                     DVWHIEKEGRAYFQVMSPIMDVVNVSNKPALYEELLQINDQLFNVAFTLYNNWVWLKT
                     LREVDSLDENEIYAQITRVGNYADRYDDELKQKYNAFQPPRTGEQA"
     gene            5531..6022
                     /locus_tag="PLF48_04545"
     CDS             5531..6022
                     /locus_tag="PLF48_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48625.1"
                     /translation="MNKKKLKIDFKIWLISSMYFIISVITSFSQTITITMKSFEYHYE
                     YINLNIVKTNHIKTATAYVTVFDFKDTLQFEKRFIFDTTGCCYQIVYTSLNKNKTNDY
                     VTYFINNFDILSQFSKKASKNTEIKFSFSKTPIEKEITLLKKYKIKEFVVENSSLYAE
                     YEQ"
     gene            6085..6432
                     /locus_tag="PLF48_04550"
     CDS             6085..6432
                     /locus_tag="PLF48_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48626.1"
                     /translation="MAKKDILDIFYEYNYTNHTFNSYCNYKDKKLLMSKGELSNEMHL
                     INETFYWLPYNEISYADLVNSTPKAVINYSVDQMGLRQMIKVYDSDFQTEKNKANNTL
                     PLTSIVKVSYEKF"
     gene            complement(6464..7660)
                     /gene="mnmA"
                     /locus_tag="PLF48_04555"
     CDS             complement(6464..7660)
                     /gene="mnmA"
                     /locus_tag="PLF48_04555"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNF48627.1"
                     /translation="MKKQRVIVGLSGGVDSSVAAYLLLQQGYEVIGLFMKNWHDGSVT
                     IQGECPWVDDSNDALLVAQKLGIPFQTLDLSEEYKKRIVDYMFAEYEAGRTPNPDVLC
                     NREIKFDIFLKHALTLGADFVATGHYCRKAITKDAEQQACFHLLAGKDGNKDQSYFLC
                     QLTQAQLSKALFPIGELTKLEVRAIAKVQSLITAEKKDSQGLCFIGKVKLPEFLQQQL
                     QPKQGDVILIPDKEINTIYSPIDNCTPEQLETYTRGNHFSATDGKKIGNHNGAHYYTI
                     GQRKGIGIGGFELPLFVVQTDTKNNLLFLGEGEHHYALNRKGLFIENQDIHWIRPDLT
                     LENGQQASYTARIRYRQALEKCCLYKTEEGMYIIFENLQKGIAPGQFCAWYEGEELVG
                     SGVIAR"
     gene            7909..10122
                     /locus_tag="PLF48_04560"
     CDS             7909..10122
                     /locus_tag="PLF48_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF48628.1"
                     /translation="MRILFTIALFLSISVHFIAIAQTKEIKDTIIVNKLNEVVISATK
                     LEESKHKIIQQISIIKSNNIEKVNAQTTASLIENQGHAFVQRSQAGGGSIILRGFEAS
                     RVVMMIDGVRMNNLIYRAGHLQNIITIDNSILDRVEILNGSSSTMYGSDALGGVISMY
                     TRKPLLQSSDKILIKGNTFFRYGLANNELTGHAEINIGLKKFASLTAVTYSKFGDLRM
                     GGAQNPFYKKFGERTFYADRIDGNDTLITNNNPLIQKKSGYSQYDILQKFLIKQNDFL
                     QHQLNIQFSNSTNISRYDRLTDPKGNGLNSAEWYYGPQTRILTAYNIEYNHPNAFFNS
                     IHANISYQYVVESRHNRSFGKNNLSNRIENVHLVGYSLDIVKQIKTNKIIFGVDGQYS
                     MVISKANKVDITTGEQSPLDTRYPNGKDNMTTFAVYATHIGKYGKYFTINDGIRIGVS
                     SLLAHFNDTTFFKFPFQSVQQNNFTASGNLGFAYAPTNKWKIALLFNTGFRVPNIDDL
                     SKVFESAPGKIIVPNNKIKPEKTISGELNISKQFGNSVKWENVFWATYFIDAIQTGKF
                     SYNGLDSIDYADTKSAVYANQNTGKAYLLGFSSSISADFSQYFSASFSINYTRGRILS
                     ETRQVPLDHIAPLFGRIGVNYHAKKVFVELYSLFNGWKKLKEYSDSGEDNLQYATKDG
                     MPAWFTLNLKAAYQPHRNITLMAGFENLLDTQYRTFASGINSAGRNIYLTLKVNW"
     gene            10197..>10722
                     /locus_tag="PLF48_04565"
     CDS             10197..>10722
                     /locus_tag="PLF48_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48629.1"
                     /translation="MYNKNNKQQVEFWNMMQIRVQHLIKYCFYYAVIAIFFVSAKTDA
                     QTLPADKIDSTEFLKGKDYDVYGNSKRFIENIGQYGDVYDKYPEMGNILYAYEGFNVP
                     ILFTPKGLIFLHRKIKKPALSEQEREEREKKKKKKEEEIEELKVVDRSISMEWINANP
                     NVQIAVSDKTEEYHT"
BASE COUNT         3578 a         1898 c         1790 g         3456 t
ORIGIN      
        1 taatagggat ttatcaaact ccagtgtaat aaaatttcct gcaggattgg gaaaaatatt
       61 gaatggcaat gtctgagttg gatttgagtt aacagcagtt actatcggga ttttttcaga
      121 ataataccat aagcctccat tgtaattacc aactaatatt tctaatttgt tatcactatc
      181 taaatcagca atagcagtag tactgcgagc aaaaatttta tttcccagca acatacccgt
      241 tggtattaaa gaatcgttcg gctgatgtga actgtaattg ttataccagt ttattccgat
      301 actattactt cctataacta aatcctgcaa accgtcgtgg ttgatatctg caacagtagg
      361 ttgtgcatat ccaaaattat tgtaaaggct atttgttctt atctttccaa aattagatgt
      421 aactaaagca agatttattg agttgtttcc gctaccgcta taataagata tacgtccgtt
      481 tttgtctcct atgagtaaat cattattgcc atctccgttt acatcagcaa taaaaggtgc
      541 agcttgttgc tctactaaga tatccgttgc agcattgtct ttcagcaatc cgcgaaaaac
      601 aaaaacaggt atatttccat tgccggccgt attttcccac cataagatat ttccagtatt
      661 aaatcctgca attatatccg tatcgccatc attatcgata tcacccgcat tcggtatcaa
      721 atctctaata ccaaacgtag atacatccag ataatcggcg tttaccaatt caaattttgg
      781 cagtgtatca gagccaatgt ttttataaag ttctaacgtg tattttatgg gatgaatatt
      841 gtctttgtag ccgccattgc caattaatat gtcttttagt ccatcaccgt tatagtcgaa
      901 aatacaaggt gctgaatttt caccattatc aatgatatca gttagtaaga agtttttggt
      961 ttgcaaggaa agattaaaag aatctgcaga cagattttta taccagtaaa tattatcgat
     1021 attattggaa ccaacatcaa atgtagaaac aatgagatca tttcttccgt ctccgtctgc
     1081 atctagtttc attggtgttg ccatagatgt tgcactgaat gaagtattgt acgaaggata
     1141 tctggcatct tgcttcaaaa tgcttgcata agaaaatgag cccatgttat acaaagcagt
     1201 aatattttgt atgccaatat tcccaattag taaatcaact tttccgttat tatctaaatc
     1261 ggcagactcc ataaacgagc cggtatgcaa aatctgatcg gggctgccta agcggaattt
     1321 gcccatgcaa gtatcgcgga tggaataggt ggcagaaaag gtatcacgta catttcccca
     1381 gcaatgatct accagattaa acgtaaatgt atcacatgga agactttttt ctacccgctg
     1441 attttcatag taataaagat aggtaccaaa tgtttcaaag ctgatgaaat ccaaatctcc
     1501 gtctttattt atatcctcta taattggtct gctgacatca accgcataaa catttgtaat
     1561 gccattgtta taaaaaaaac cattttgttg caatttgaat ttcaaagtat cttgatcgta
     1621 atagccttta aatactttag tattagaaag tccgttatag gtaaacaaat ccggtatggc
     1681 atcacagtta tagtctttca aaatcatcca gttgctgata tccggaaatt gtgctgcata
     1741 ctgaggtgca aacgtataag ctgtagagcc aacagttttt aaaagaaaaa caaaaaactg
     1801 acggttgtaa ttatcataaa caacgatatc ttttttgccg tcatggttga catccgcatt
     1861 agcgaattgt gcattattca atccacccat ccagggataa ggcaataagc tactgttttt
     1921 cactacttct acatcataat gcttgtaata agtttgcgca tccgtattcc agataaaaaa
     1981 cagcactaaa aacagcagac taagtaggta tttcattatg actaagatac agaaatttta
     2041 aatgggattg tttgattaaa agtaattaga gttgatattt tattgcgaat aaacggttgt
     2101 tagcttatcg gatgtaaata ttgcattagg cgaaattact ttttctgtgt aggggatttt
     2161 attgccatag cgttctttta gttttttgct tacttgttta ttttttaaat ccaatgaaga
     2221 caatggctgc aagctgccca attttattcc tgttgcattg aaataggctt tccataccaa
     2281 ttcagaacaa tatattttgt tatccgacca ttcaaatgta taatcataat tttttcctaa
     2341 gaatttcata gcttcatttt ttattgcatc ttgttgattt attgtaatag cagcacgctt
     2401 aattactgca tgttttgctt ttccccgggc tatccattta tccaaaggtg tccattttac
     2461 aggttgtact gcttcgagta cataccagtt tttatcattc gcatttctat gaaaaagcag
     2521 accacaatga ctataaactg aatgtgtggc tgactggata gccctgcttt gtgatgatgt
     2581 tgaagtatga aaaaggatat ccccttcttg aaaaatatct gatttactgt ttagctcaac
     2641 caaaggcatt ttacccgagt taattgcatt acttgtatgg tgtttacaat tttcaaacaa
     2701 cagcaggcaa acaactatga ctatgaccga attatatttc actacagatg taaacgaatt
     2761 tttgtacgga aaagtataaa aaaagtagcc gccgcccttc gggcggcggc aacaaatctg
     2821 attttattat ttactttgaa atatcatcag tcttttttca actcatgact tatgctttca
     2881 aattcttaat gctgactggc tttgaaagct ttaatagctt ctgttagttt aggtacaact
     2941 tcaaatgcat cgccaactat accatagtct gctgctttga aaaatggtgc ttccggatct
     3001 ttattgatta ccacgataca ttttgattgg tttactcctg ctaaatgttg tattgcaccc
     3061 gaaataccga ttgctatata caagtttgga cgtatagcta caccggtttg tcctacgtgt
     3121 tcataatgcg gacgccagtg aacatcagct acgggacgtg aacaagctgt agcagcaccc
     3181 aatactttag ccaaatcttc tatgataccc cagttttcag gtcctttcat acctcgacca
     3241 gcagaaacta ctaattcagc ttcaggtaaa ggagcaatat ctcctgtttg tcgactgatt
     3301 tctttaaccg ttattctgga tgcagccggt gtaaggtcaa cagcttcaac tgtagctgtc
     3361 ccctctcctt ttacagcacc aaatgcattt ggagtaacag atactacttt cttaggtgtg
     3421 ttaatattta caaaagcaaa tgctttaccc gaaaatgctc ctcttttaac aacaaatccg
     3481 ttagtcatat ccggtaagct tacgacgccg gaaacaatgc cggcgtctaa acgcacggct
     3541 actcttgggg caactgcttt tcctgttaaa ctcatagaca ttacgacaac atctgcgcca
     3601 gctttatctg cggcatcggc tatggctttg gcatatactc cggagtcaaa attatcaaaa
     3661 gcactatttg aggcatgcaa tactttatta gcaccgtaga tgcccaaatt tgcaagttcg
     3721 acatcattgg cagttcctaa tgcaattcct actgcatcag tgcctaattt tgaggcaact
     3781 tgtgcagcat acgaagcagc ttcaaagctg ttctttttta ctttgccatt ggctgtttct
     3841 ataaaaaata aaactgacat attatattac tttagcttcg ttatgtaata attgtaccaa
     3901 ctcgtccacg tgatcgggag atatcatttt gcaggctgct tttgcaggag gcaattcata
     3961 ttttaccgtt gtggttaatg acgcaactcc tttaggagtt actacttgta aaggtttaga
     4021 tttagccgcc ataattcctt tcatattagg aatacgggct tcagccattc ctttagctgc
     4081 tgacaataca aaaggaacat caacagacaa aatttctttt ccgccttcaa tttcacgctc
     4141 tatagtggct gtattgccag acatttctaa ctttgatgca taagaaataa aaggcaagtc
     4201 cattaattca gcaatcatac cgcccaaatt agaaccgtta ttactgatgg tttcttttcc
     4261 caataatatc atatcgtaag caccggattt ggcaacatct gcaatatctg atgcaatggc
     4321 aaaagcatcc agttcgccgg ctgcatcaat gcgtatggca tcatcagcac caatagccag
     4381 ggctttacgt attactgcat cgttttctgc cggtccaaca tttactacgg ttaccgtacc
     4441 accgttagct tctttgagtt ccagtgcacg caccaatgcg taccattcgt cataagggtt
     4501 cagaatccac tgtactttat cgttgttgaa cttcgtgtcg ttgtccacaa acgaaatctt
     4561 agtagtggtg tccggaacca aacttataca tactaaaaat ttcatacctt taaatttatt
     4621 ttgatactaa tttagctcat tttcataaat tcaatgcatg cattcgatta ggttagaaaa
     4681 gttattggct ctatatgaag aaaatccaac agatagtttc ataaactttg cactggctaa
     4741 agaatatgaa ctcaacaata acttgaacaa agccatacaa ctacttgaaa aactaatcat
     4801 tacaaaccca aattatttag ggacttattt acatcttgct aaattatatg agaaaaacgg
     4861 tgagattaaa aaagcattaa atatttatga aaagggctta attgttgcta aacatgcaaa
     4921 agatctgcat agcctgagtg agttgaaaaa tgcaaaaatg aatttggaat tggaagaatg
     4981 attttatttt ttgctgtaat tttaataact ttctaaaaat attatactat gtcagactta
     5041 caaaaaacca ttcagatggt agaaaattcc atccgcaaat taggaataga cccaagtacc
     5101 tgcaaaggtg aaaaagaagg acaatattca ttaaaccggg gttcggcagc cgtatggatt
     5161 gacgtgtggc acatagaaaa agaaggtcgt gcttattttc aggtgatgag tccgataatg
     5221 gatgttgtca atgtgagcaa caaacctgca ttgtatgagg aactgctcca aattaatgat
     5281 caactgttca atgttgcctt tacgctgtat aataattggg tgtggttgaa aacgctgcgc
     5341 gaagtggata gccttgatga aaatgaaatt tatgctcaga ttacaagagt aggaaattac
     5401 gctgaccgct acgatgatga actcaagcaa aagtacaatg cattccaacc accccgaacc
     5461 ggagagcagg cgtagaaaaa atcaacgcat ttataatgtt ttataaattc agaatacata
     5521 tttttgtggt atgaataaaa agaagctgaa aatagatttc aaaatttggc taatatctag
     5581 catgtatttc attatatcag taataacttc ttttagtcaa actataacta ttacaatgaa
     5641 aagttttgaa tatcattatg aatatattaa cttaaacata gtcaaaacaa atcatattaa
     5701 aactgctaca gcttatgtga cggtttttga ttttaaagat actttacaat ttgaaaaaag
     5761 attcattttt gataccacag gatgctgcta tcaaatagta tatacttcat taaataaaaa
     5821 taaaacaaat gattatgtta catactttat aaacaatttc gatattttaa gtcaattttc
     5881 aaaaaaagcc agtaagaata cggaaataaa attttcattt agtaaaacac ctatagaaaa
     5941 agaaattaca ttactaaaaa aatataaaat taaagaattc gtagtagaaa attcaagtct
     6001 ttatgcagaa tatgaacagt aggaaagaaa cctactatta aagagtataa aatctatgat
     6061 tctttaaata acattaaaga atttatggca aaaaaagaca tacttgatat attttatgaa
     6121 tataactaca ccaatcatac atttaattca tactgcaatt acaaagacaa aaaattactt
     6181 atgagtaaag gtgaattaag taatgaaatg catttgatta atgagacctt ttattggcta
     6241 ccatataatg aaatatctta tgcagatttg gtgaattcta ctccaaaagc agtaataaat
     6301 tactcagttg accaaatggg gttaagacaa atgataaaag tttatgacag tgatttccaa
     6361 acagaaaaaa ataaggccaa taatactctg ccattaacta gtattgtaaa agtgagttat
     6421 gaaaaatttt aaataattca aacagaaatt agtacatcaa atgttatctc gcaatcaccc
     6481 cgctgcctac taattcttcg ccttcgtacc aggcacaaaa ctgaccgggt gctatgcctt
     6541 tttgcaggtt ttcaaaaatg atatacatac cttcttcggt cttgtataaa cagcattttt
     6601 ccaatgcttg tcggtagcgt attctggctg tatagcttgc ttgttgccca ttttccaaag
     6661 tcaaatcagg ccgtatccaa tgaatgtctt ggttttcaat aaacaaacct tttctgttga
     6721 gggcgtaatg gtgttcacct tcacccaaaa aaagcaaatt gtttttggta tcggtttgta
     6781 ccacgaaaag cggcaactca aatccgccta ttcctatccc ttttctttgt cctatggtat
     6841 aataatgtgc gccgttatgg ttgccaattt ttttaccgtc tgttgctgaa aaatgattgc
     6901 ctctcgtata agtttctaat tgctccggtg tgcaattgtc aattggtgag taaattgtat
     6961 ttatttcctt atcagggatt aaaatgacat ccccttgttt aggttgcaat tgttgttgca
     7021 aaaattcggg taattttact ttgccaataa aacacaatcc ctgagaatct tttttctcag
     7081 ccgttatcag gctttgcact ttcgcgatag ctcttacttc gagcttagta agttctccta
     7141 ttggaaataa tgccttactc aattgtgctt gggttaattg gcataaaaaa taactctggt
     7201 ctttgttccc gtcttttccg gctaataaat gaaagcatgc ttgttgttcg gcatcctttg
     7261 taattgcttt tctacaataa tgcccggttg ccacaaaatc ggcacctaat gtaagcgcat
     7321 gtttcaaaaa aatatcaaat tttatttcgc ggttgcacaa tacatccggg ttaggcgttc
     7381 gtcctgcttc gtattcggca aacatataat caacaatacg ctttttatat tcttcgctca
     7441 agtccaaggt ttgaaaggga atacccaatt tttgcgccac taataaagca tcgttactgt
     7501 cgtccaccca aggacattca ccctgaatgg taacgctgcc atcgtgccag tttttcatga
     7561 acaaacctat tacttcatag ccttgttgca acaataaata ggctgccaca ctggagtcta
     7621 ctccacccga taaacctact atgacacgtt gttttttcat gaaatgagaa tgctgtatta
     7681 ctaagtcaat taattaagcg ttgcaaattt acacatttgt gaggaattgt atttcgttcc
     7741 aaattttcag taaatattaa atgcttcata atttataata agactgtcac tttttatttg
     7801 agaaaaatgt gttatcatat gtatgctatc ttcttaaaac attcagaata agctattttt
     7861 gtcataagta tttttttcac tatataaatt caacattcaa aatattttat gagaattcta
     7921 tttacaattg ctttgtttct ttccatttca gttcatttta tcgcaattgc acaaactaaa
     7981 gaaattaaag atacaatcat cgtaaacaag ctgaatgaag tagtaatatc tgccactaaa
     8041 ttagaggaat ctaaacataa aattatacag caaatcagta tcataaaatc aaacaatatt
     8101 gaaaaggtta acgcacaaac tacagcatcc ttaatagaaa atcaaggcca tgcttttgta
     8161 caaagaagcc aggcaggagg tggcagtata atattaagag gatttgaagc cagcagagtg
     8221 gttatgatga ttgacggtgt tcggatgaat aatttaattt atcgtgccgg acatttgcaa
     8281 aatattatca caattgacaa ttcaatttta gatagagttg aaattctgaa cggttcgtct
     8341 tctacaatgt atggtagcga tgctttaggt ggtgtgatta gtatgtacac cagaaaacct
     8401 ttactacaat catctgataa aattttaata aaaggaaata catttttcag atatggcctg
     8461 gcaaacaatg aattaaccgg gcacgcagaa atcaatattg gtcttaaaaa atttgcttca
     8521 ttaactgcag ttacttatag taaatttggt gatttgagaa tgggtggcgc acagaatcca
     8581 ttttataaaa aatttggcga acgcactttt tatgctgaca gaattgacgg aaatgacact
     8641 ttgattacga ataataatcc tttgattcag aagaaaagtg gctattcgca atatgatatc
     8701 cttcaaaaat ttttgataaa acaaaatgat tttttacagc atcaactcaa tattcaattt
     8761 tctaacagca caaatatttc tcgatatgac agattaaccg atccgaaagg aaacgggtta
     8821 aattctgctg aatggtatta tggtccacaa acacgaatac taactgctta taacatagaa
     8881 tataatcatc caaatgcatt ttttaattcc attcatgcaa atattagcta tcaatatgta
     8941 gtagaaagta ggcacaatag aagttttggc aaaaacaatc tttctaacag aatcgaaaac
     9001 gttcatctag taggctattc tttagatatt gtaaaacaaa taaaaactaa taaaataatt
     9061 tttggtgttg acggacaata cagcatggtg atttcaaaag ctaataaagt tgatattaca
     9121 acaggcgaac aaagtccatt agatactaga tatccaaatg gaaaagacaa tatgactaca
     9181 tttgctgtat atgcaacaca tatcggaaaa tatggaaaat attttactat taatgatgga
     9241 atcagaatag gtgtttcatc attgctggca catttcaatg atactacttt ctttaaattt
     9301 ccattccaat ctgtacaaca aaataatttt acggctagtg gaaatttggg cttcgcctat
     9361 gcacccacaa ataagtggaa aattgcatta ttatttaaca caggttttag agtacctaac
     9421 attgatgact tgtctaaagt attcgaatct gcacccggaa aaattatagt tccaaataat
     9481 aaaatcaaac cggaaaaaac aattagcggt gaactaaata tttctaaaca atttggaaat
     9541 agcgttaaat gggaaaatgt tttttgggca acttatttta tagatgccat tcaaactggg
     9601 aaattcagtt ataatggatt agattctatt gattatgctg atactaaaag tgccgtgtat
     9661 gctaaccaaa atacaggtaa agcctattta ttgggattta gttcttccat ctcagcagat
     9721 tttagtcaat attttagcgc ttcattttca attaattata ctcgtggtcg aatattatca
     9781 gagactcgtc aggtgccctt agatcacatc gctcctttat ttggtagaat tggcgtcaat
     9841 tatcatgcta agaaagtatt tgttgaactc tattctttat ttaatggctg gaagaaactg
     9901 aaagaatata gtgatagtgg cgaagataat ttacaatatg ctacaaaaga cggcatgcct
     9961 gcgtggttta ctttaaacct caaagcagct tatcaaccac atagaaatat tacgctaatg
    10021 gctggctttg aaaatttatt agatacacaa taccgaacat ttgcttcagg cattaactcc
    10081 gctggaagga acatatatct gacactgaaa gttaactggt aattcacata gattaaaatc
    10141 atgatataag tcaataggtt cttgtataaa tagtagtaat tttatttttc tatcagatgt
    10201 ataataagaa taacaaacaa caagttgaat tttggaatat gatgcaaatt cgagtccaac
    10261 atttaataaa atattgcttt tattatgcag ttattgctat tttttttgtt tctgcaaaaa
    10321 cagatgcaca aacattgcca gctgacaaga tcgattcaac ggaattttta aaaggaaaag
    10381 attacgatgt ctatggaaat tccaaacgat ttatcgaaaa cattggacag tatggtgatg
    10441 tgtatgacaa atatcctgaa atgggcaaca tcttatatgc ttacgaagga tttaatgtac
    10501 caatattatt tacaccaaaa gggctcatat ttctgcatcg caaaataaaa aaacctgctc
    10561 tgtcagaaca ggaaagagaa gaacgggaaa agaaaaaaaa gaaaaaagaa gaagaaattg
    10621 aagaattaaa agttgtcgac cgttccatat ccatggaatg gataaatgca aatcctaatg
    10681 ttcagattgc tgtatcagat aaaactgaag aatatcatac tt
//
LOCUS       DAOQII010000063        10688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3198_length_10688_cov_6.419261, whole genome shotgun sequence.
ACCESSION   DAOQII010000063 DAOQII010000000
VERSION     DAOQII010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3198_length_10688_cov_6.419261"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLF48_04570"
     CDS             <1..473
                     /locus_tag="PLF48_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNF48630.1"
                     /translation="LNAPEAEIQLSGDEYIVGRQLKPEARKDIIGFFAENQLVPTAMI
                     DISDGLSSEILHICKQSNVGCQINEAAVPIAEETYNKALEFNIDPINCALSGGEDYEL
                     LFTIKPDDKAKIEQNENIHIIGEMLDANLGVKLYSKGGNYYDIISLGWDGLKQE"
     gene            complement(742..1092)
                     /locus_tag="PLF48_04575"
     CDS             complement(742..1092)
                     /locus_tag="PLF48_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48631.1"
                     /translation="MKKRINRILENTLICVLIILVVIKLINDEITKKQISKKSGITVC
                     TIVNYYEIYGSEYAEYQYRVGLNTYVKKSGVDPYSGKFDRNNLKSNIGILRYVKYSIK
                     DPERSILLYDSIPQ"
     gene            complement(1571..1870)
                     /locus_tag="PLF48_04580"
     CDS             complement(1571..1870)
                     /locus_tag="PLF48_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48632.1"
                     /translation="MKDEIIKMIVETEGKHPNDPTITTKLNSITEILSKSLDETISCL
                     NELDENSISWISTRFENLSYKFQSKEFIDCIKNLVVKFPNISYLKDDVQDAIDAM"
     gene            2287..2577
                     /locus_tag="PLF48_04585"
     CDS             2287..2577
                     /locus_tag="PLF48_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48633.1"
                     /translation="MAFLVNHVAVFLYSSPFVLIQKWSKKSRTIKGIFACACGFSSKK
                     SACENYLVTLVSGCRFIGKFAKQPIYLRCVYTIVLSRNDFLSPHAVYCSAKL"
     gene            complement(3174..3566)
                     /locus_tag="PLF48_04590"
     CDS             complement(3174..3566)
                     /locus_tag="PLF48_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48634.1"
                     /translation="MFFFVSIFSSNYIIAQTRSDTIYLGCVNISDSLVKYIDVGLIDQ
                     GNILFCIDLINQLDSIEIYSITGSSGGMYAVDDYKKKLLNKESSSVLFYFQTKTHNGP
                     FFKPLTVLYFDKNKNKKIYPIALKGNIK"
     gene            complement(3589..5508)
                     /locus_tag="PLF48_04595"
     CDS             complement(3589..5508)
                     /locus_tag="PLF48_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48635.1"
                     /translation="MQTNNNNEVNIKIINTQSPSFIKANINKLIYMLYGINPIGNKYF
                     QISYNDVGGFNCIKTDRTNANFSGNVIKGKEDATKKVTEFLKQKNELMTRNFKNENIT
                     FFPISYMKPVATIPNYVDGKNGSILQSWSVIYKITIPAYTNKDNQQEKVDILNADIAI
                     TMSTNGQIVRVNYSLLPLQDIKSSTLNKVVAEEDTIPQIIYLLNKDIQNITPFYLSVT
                     ETAYIPATNDSIVPKQTISQPDIPFINPMEYDENRVVMWLRTSNTSKVLLAKDATDEE
                     KKKNAANTLPIGAKVIRLQPKSETSETTPCLYFWNDKYNVTDIPERNLSNKNSWLSDK
                     SGDVLRNDISHTLARVQSKVQDLKNQKENPNKDFTNLYDFVDRYKNIQLTKDQILDAQ
                     ILFNNRTKINTQDKPETVKRLTDLIKKVYNPTINKNSKTFFFEETLIVPLSEEWFLND
                     SVLTERYLQTWKKDGKLYNISGNRDDSSGAYAYLSEELTAFRNEVPKLKEIVRNSKSS
                     STPEAIWLESISDSYLSKIKRIIYIHQGTWYINQTQRYAEDVGLEASFAIFRTPALLT
                     VLLHEFSQGDNTFYRDVTGDTHVPRLNTELSHKKIIASGLTEFSQDDINYIYQVNGVI
                     SIISIAILLNIINGK"
     gene            complement(5576..8944)
                     /locus_tag="PLF48_04600"
     CDS             complement(5576..8944)
                     /locus_tag="PLF48_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48636.1"
                     /translation="MTTLEKKTTNELFRFVNTKQIGLDTIEEIDSHYIQQQYQSLSAT
                     QLSDIESAVALFYTTYLSLIGANSTVTGSTTDAVKNAADAYTLLFTDEQSFTSQFPHL
                     LKIVRWVDEHKETMTKQEFIYMVEAELGTSISTFVLGSTGAPASFMGGNAILPDVSNN
                     TYLTYWFRLWDNLIIHTCQLKNPSCIHLSSQAIVLLALLKRIASGDASLNEHKDFLDA
                     FTATPLLPLNINQLFMAYHQSNGGGNQTANTFANKTANSFTNSTANKIANAKSQYGNL
                     LTVLNEVKALQQKKRLELNSKRLEAALNSTTNTNNLTQPSENIPVVDDGSGSGTGTTG
                     GTRPPIIIEGGTEDPVVLLNTYTLTEADMASLSAETIAIIQQYAHGVTNITFQELLDI
                     INGLITGQIKTIAEGSTKVGTIRIGNVIIRSDEYCTDMAEENIPVCTILEKKDFKSEG
                     ALANYASIGDLIVTKQQLIKYDLGEVAHVENIMQGEERSRSFITTDRLEELTLTETET
                     ENETVSDTQTTERFSVEKESTSVINDQKNINFGVNISADFGPVQASTSFGYSSSHSSN
                     QSNHDATSFSKDVTTRALKRVKEKVRTLRSRLQIHEVVDKTKHKYVNIPGEDHINGVY
                     RWLDKYYLNKVLNYGKRLMLEFTVPEPASFYIFNKMLKAKGSNDYKEMPTDPNDLKED
                     IKNVDGDLITTLKLNSFKDISKENYEYFAAYYNLTDVEAYPAEDKTIGVAYDVKITGN
                     ADGALKDDSMVVPEGYKAKSFKVAYELADDNNNQSGIRIQIGSNIIRNVSHKTLNKST
                     DFNIPLANLNIPSSNNEYTLNNTIRGNDKIPVSIKAWCSNGARTVDAIVNVQVKCELD
                     ESGIDKWRLKLYNAIMEKYQQDMRDYEEWLRAQEIYDLPDMGNNPDINRNIEQTELKR
                     RCLEMFSGQRFESFDATVNGIPNVSSYPEFDFMEAIKEGSMARFFEQAFDWKNMTYEF
                     YPYYYARKKMWLTLNNIQEKNDPVFESFLKSGYARVVVPVQPGFELFLALYAGWSWLN
                     KGESIPLPLALLFYSGKGMPNVGIDHPLFVSVMNELSQIQTLNEDEPIEVGHYIQKVP
                     TNLVYLAKQHLVAGESTTADLPDHSSDPDLIDYL"
     gene            complement(9249..9698)
                     /locus_tag="PLF48_04605"
     CDS             complement(9249..9698)
                     /locus_tag="PLF48_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNF48637.1"
                     /translation="MINLEEIKNSEGLVLTAYKRKGDVWTIGYGSTYYKNNTRVKQGD
                     KITAAQAEELLHYKIEKEYLPQLKQQIKAVLNDNQLSALLSLIYNIGVGNLRRTKLLA
                     LINDDPDDEGIRLIWENTHVRKNTQFEKGLRARRKRETALYYKPVDM"
     gene            complement(10086..10268)
                     /locus_tag="PLF48_04610"
     CDS             complement(10086..10268)
                     /locus_tag="PLF48_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48638.1"
                     /translation="MKNMNRLTPEEITNLIQEGLNLLIKGFENIGFRRKTNERLQLLE
                     DQVALLSKKLLASEES"
     gene            complement(10306..>10688)
                     /locus_tag="PLF48_04615"
     CDS             complement(10306..>10688)
                     /locus_tag="PLF48_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48639.1"
                     /translation="MKNQKAYSVLISLIPAIQAILDTFNAAKVDGAMRTYIGASLMLL
                     LIILQGIQIYLNPNIKDRALWVSIVALCGYIAGGVLDNLNLIHLTDEVFSVVRLTFSL
                     LVVIANAIVREYNTMPITTKESDK"
BASE COUNT         3015 a         1897 c         1798 g         3978 t
ORIGIN      
        1 ttttaaatgc acccgaagcg gagatacaac tgagtggcga tgaatatatt gtaggcagac
       61 agctcaagcc ggaggcgcgt aaggatatta tcgggttttt tgcagaaaat cagcttgtgc
      121 cgactgcaat gatagatata tctgacggat tgagttcaga aattctccac atctgcaagc
      181 aaagcaatgt gggttgtcaa atcaatgagg ctgcagtgcc cattgccgaa gaaacgtata
      241 acaaggctct agagtttaat atagacccga taaactgtgc tttaagcggg ggagaagatt
      301 atgagttgtt atttactatt aagccggatg ataaagcaaa aattgaacaa aatgaaaaca
      361 tccatattat cggtgaaatg ctggatgcta atttgggagt aaaactctac tcaaaagggg
      421 gcaattacta tgatattatt tccttaggct gggatggctt gaagcaggag taatttatac
      481 ccggaaatgg tgttataatc tgaatttatt gcttcgtgaa cggaacacca aaagcgaact
      541 tagagggctg ggaaaacaac tttttctgat gggtattctt ataactcttt agggttcgta
      601 gtttgttggt tacaaaccaa caatgacaag ggggcattac ttcattatct aaaaaactgt
      661 cataatcaaa ctgagcgaat ttgagacaga caggcgaata cttccatatt acttaaagtt
      721 ctatatggtt gctgtagagg gttattgagg aatcgagtca tataataata tcgaccgctc
      781 tggatctttt attgaatatt ttacatagcg taatatacca atatttgatt ttagattatt
      841 tctatcaaat ttaccagaat aagggtcaac tcctgatttt ttcacatatg tatttaaccc
      901 aactctatat tgatattctg catattcaga accataaatt tcataataat tcacaatagt
      961 acatactgta attcctgact tcttagatat ctgcttttta gtaatctcat catttattaa
     1021 ttttattact actaggataa taagtacgca tattaatgta ttttccaata ttctatttat
     1081 tcgttttttc attacttttt atttttatta tatcctagct catactgttc gcccacgtta
     1141 ttgtttagca tagcagaagt caaagcgtgg tatgcgtcac caacaaacca ttatccgttt
     1201 ctcattcatc atcatgcgat aaaaaatccc agtcatttat atttaattga taaaattttg
     1261 cactgctgta tttgtattcc tctataaaaa tagttgattt tttttatcaa aaaactcctc
     1321 cttgtaattc ctcctcctaa tgtaggtgga ggagaaaagg agaaatcaac tatttttttt
     1381 gcctttcttt atagcaagta actgttataa aatccagatg atattctttt attcgttctg
     1441 ttatcactta gctaaaatat aagatttttg gcacgatggc ttagtgaaat aaaagttgtt
     1501 ggtgacgctt gctgcgctta ttgcatggct aagctaaaca ccaatcaacg gcgaatgtag
     1561 tgagctacaa ctacattgca tcaattgcat cttgtacgtc gtctttcaaa taactaatgt
     1621 ttggaaattt cacaactaaa ttcttaatac agtcaataaa ttctttactt tgaaatttat
     1681 atgataaatt ttcgaatctc gtagaaatcc aacttattga attttcgtca agctcattta
     1741 aacaactaat cgtttcatct aatgattttg ataaaatctc tgtaattgaa ttcaatttcg
     1801 ttgttatagt tggatcgttt ggatgtttgc cttctgtttc tactatcatt tttattatct
     1861 catctttcat aatattatta ttttatatga ttaattcaaa aatttgtact ttttggcatt
     1921 ggttgaatag cattatctgg aaaaatagtt gcaggtttac catcctagct cgctcctctc
     1981 gcctttggtg gtgtttagca tcgctaagct ttaagcgttg gatgcgttac caacaaacca
     2041 ttatccgttt ctcattcatc ataatgtgat aaaaaatccc agtcatttat attcaattca
     2101 taatttttgc actactgtat ttgtattctt atttaaaaat agttgatttc gtttttttta
     2161 gaaccaccac cttttatttc tcctacttga tgaaagtgga ggaagtaagg ttaaatcaac
     2221 tattttacag ttgactaacc ttaacggctt tgtgtataaa tatacaattt ttatcttgtt
     2281 tttgggttgg cttttttggt taatcatgtt gccgtgtttt tgtattcttc cccttttgtc
     2341 ttgatacaaa agtggagcaa aaaatcaaga acaataaaag gcatttttgc ctgtgcttgt
     2401 ggcttcagct ctaaaaaatc tgcttgtgaa aactatcttg tcactttggt tagtggttgt
     2461 cgttttattg gcaaatttgc aaaacaacct atttatttac gttgtgttta cacgatagtt
     2521 ttaagtcgca acgatttttt atcgcctcat gctgtttatt gttcggctaa actttaacgg
     2581 gttgcctttt ttagtaatta tactttttct gtatttatac tggtttaaga attcagttta
     2641 atgtaaattg tttgttgatg ttgctgtgca gcgagccact gggttggcag gtgttatcac
     2701 ctgccaacca cgaaccgtat tgaaaacact tcccaatcgg ttggtattct caccaactga
     2761 aacaaacaaa aattttaatt tttcaaagtt attcatttta ttgtaactta gatttatata
     2821 ctgagaaaac taaagttgaa gtatagttta accattataa aacactacac aggaatatta
     2881 ggttaaacta atacttatgg agggagagaa aataagataa tgtgttgtat tttacccctc
     2941 gctctgtttt gaaaaaatgt tattttatgc cccccataaa ttgcatttaa catttgaact
     3001 tgggttcttc acattaattg gggatgtatt ttgcaaatca gactcgccta tgcatatatt
     3061 atatgaataa taatttatta tgctatcgtt tgcttatgca aacgatagca taatatctat
     3121 aaaaattaaa ataacgctaa tttttttcat ctgtattgct taaacaagca cgtttatttt
     3181 atattaccct ttaatgcaat tggatatatt tttttattct tatttttatc gaaatacagt
     3241 acagttaatg gcttaaaaaa aggaccatta tgcgtttttg tttgaaaata aaacagtaca
     3301 gacgaagatt ctttgtttaa taacttcttt ttataatcat caacggcata catcccgccc
     3361 gaagaaccag taatactata tatttcaata gaatctagtt gattgattaa atcaatacaa
     3421 aataatatat tcccttgatc tattaatccc acatcgatat attttaccaa actatcagaa
     3481 atattgacac atcctaaata tattgtatcg gatctcgttt gggctataat gtaattactg
     3541 ctaaaaatac ttacaaaaaa aaacataaaa aaaaattttt tattcatatt atttaccatt
     3601 tattatgttt aataaaattg cgatactaat tattgaaatt acaccattta cttgatatat
     3661 ataattaata tcatcttggc tgaattcagt taatccgctt gctattattt ttttatgcga
     3721 caactctgta tttaacctag gcacatgtgt atcacctgtt acatctctat aaaaagtatt
     3781 atccccttga ctaaattcat gtaataatac tgttaataag gctggtgttc tgaatattgc
     3841 aaatgaagct tctaaaccaa cgtcttccgc ataacgttga gtttgattaa tataccaagt
     3901 accttgatgt atatatataa ttctttttat tttacttaaa taactatcgc taatactttc
     3961 taaccaaatt gcctccggcg tggaagaaga tttagaatta cgtacaattt ctttgagttt
     4021 gggaacttca tttctaaatg ctgttaattc ttcactgagg tatgcgtatg caccagaact
     4081 atcatcccta tttccagata tattatataa cttcccatcc tttttccagg tttgcaaata
     4141 tctttcagtt aatacagaat catttaaaaa ccattcttca ctcaatggca caattaaggt
     4201 ctcttcaaaa aaaaatgttt tactattttt atttattgta ggattatata ctttttttat
     4261 tagatcagtc aatcttttta ctgtttcagg tttatcttgt gtatttattt tggtgcggtt
     4321 gttaaacagt atctgcgcat caaggatttg gtctttagta agctgaatgt ttttatacct
     4381 atccacaaaa tcatataaat ttgtaaaatc tttattgggg ttttcttttt gatttttcaa
     4441 atcttggact ttggattgta cccttgccaa tgtatgagaa atatcatttc ttaatacatc
     4501 acctgattta tctgaaagcc aactgttttt attactcaaa tttcgttctg gaatatctgt
     4561 tacattatac ttgtcattcc aaaagtataa acaaggtgtt gtttcagaag tttcagattt
     4621 tggttgtaag cgtattactt tggctccaat tggcaatgta tttgcagcat tttttttctt
     4681 ctcttcatca gtagcatcct tggctaatag cacttttgag gtattgctag ttcttaacca
     4741 catcacaact ctattttcat cgtattccat tggattaata aaaggaatat caggttgaga
     4801 tattgtttgt ttgggtacga tagaatcatt agtcgctggt atatatgccg tttctgtaac
     4861 agacaaatag aatggtgtaa tgttttgtat atctttattt aataggtaaa ttatctgagg
     4921 tatagtatct tcttctgcaa ctactttatt taatgtagaa ctttttatat cttgtaatgg
     4981 aagtaagctg taatttactc taacaatttg cccattggta ctcatggtaa ttgcaatatc
     5041 tgcatttaag atatccactt tttcttgttg attatcttta tttgtataag ccggtattgt
     5101 tattttgtaa ataacagacc aagattgtaa aatacttcca tttttaccat ctacataatt
     5161 tggtattgtt gccactggtt tcatgtatga tattgggaag aaagtaatat tctcattttt
     5221 aaaatttctt gtcattaatt cattcttttg ttttaaaaat tctgttacct ttttagttgc
     5281 atcttccttg ccttttatta cattgcctga aaaattggca tttgttctgt cagttttgat
     5341 acaattaaac ccaccaacat cgttataaga tatttgaaaa tatttattcc caataggatt
     5401 tattccgtac aacatgtaaa ttaatttatt aatatttgct tttataaaac taggtgattg
     5461 agtgtttata atctttatat ttacttcatt gttattattt gtttgcattt tatttcattt
     5521 ataaagttaa aaaaatagtt agcgcgcttg cacgcgctaa cgtgttaaag tgttgttata
     5581 aatagtcgat aagatcggga tctgatgaat ggtccggtaa atcggctgtt gtagattcac
     5641 ctgctaccaa atgttgctta gctaaataca ccaaattggt cggcactttt tgtatgtagt
     5701 gccctacttc tatcggctca tcctcattga gggtttgtat ctgtgacaat tcgttcatca
     5761 ccgatacaaa caatggatga tcgataccca catttggcat tcccttgccg gagtagaata
     5821 gaagggctaa cggcaatggt atggattcgc ctttgttgag ccaagaccag cctgcataca
     5881 aggctaaaaa caactcaaag cccggctgta ccggtactac tacacgtgca tagcctgatt
     5941 ttaagaaact ctcgaatacc ggatcgtttt tctcctgaat attgttgagt gtcagccaca
     6001 tttttttgcg ggcatagtaa tacgggtaaa actcatacgt catgtttttc caatcgaatg
     6061 cctgctcaaa gaatcgagcc atggagcctt cttttatcgc ttccataaaa tcaaattcgg
     6121 gataagaaga aacatttgga atgccgttta ctgttgcatc aaagctctcg aaacgctgac
     6181 cgctgaacat ttccagacaa cgacgtttca gttcggtttg ttctatgtta cggttgatat
     6241 ctggattatt gcccatatcc ggcaaatcat atatttcctg tgcacgcagc cattcttcat
     6301 aatcgcgcat gtcttgttgg tatttttcca taatggcatt gtataatttc aatctccatt
     6361 tatcaatacc cgactcatct aattcacatt ttacttgcac atttacaata gcatctaccg
     6421 ttctcgcccc gttagaacac catgctttaa tggacaccgg aattttatcg ttaccacgaa
     6481 tagtattgtt taatgtatac tcattattag aagatggaat atttagattt gctaacggaa
     6541 tgttaaaatc tgtagattta ttaagagttt tatgacttac atttcgaata atgttggagc
     6601 caatttgtat cctgattcca ctttggttgt tattgtcatc tgcaagttca taagctacct
     6661 taaaagattt agctttatac ccttccggta ctaccatact atcatctttt aaagcaccat
     6721 cagcattgcc tgtaattttc acatcataag ccacaccgat agtcttgtct tctgcagggt
     6781 aagcctctac atcggtcagg ttgtaataag ccgcgaagta ttcataattc tctttactga
     6841 tatctttgaa cgaattgagt ttaagtgttg tgattagatc tccatcaaca ttcttaatat
     6901 cttctttcaa atcattcgga tctgttggca tctctttata atcattagag cctttggctt
     6961 tgagcatttt attgaaaata taaaaactgg cgggttcggg tacggtaaat tccaacatta
     7021 ggcgtttgcc ataattcaac actttattca ggtaatattt atccaaccaa cgatacacgc
     7081 cattgatatg gtcttcaccc gggatattga catacttatg ttttgtttta tccaccactt
     7141 cgtgtatttg caatctggag cgcaaggtgc gtactttttc ttttactcgt tttaatgctc
     7201 ttgtggttac atctttagaa aaactagtgg catcatgatt ggattgattg gagctgtgtg
     7261 atgagctgta accaaaactt gtgctagctt gcaccggacc aaagtcggca cttatgttga
     7321 ccccaaagtt gatatttttt tggtcattga taacacttgt tgattctttc tctacactaa
     7381 agcgttcggt tgtttgtgta tcactcacgg tttcattttc cgtttccgtt tccgttagtg
     7441 tcagctcttc cagcctgtcg gttgtaataa aacttctgct tctttcttcg ccctgcataa
     7501 tattttctac atgtgctacc tctcctaagt catattttat caactgctgt ttggtaacaa
     7561 tcaggtctcc gatactggca tagtttgcta atgcaccttc gctcttgaaa tctttttttt
     7621 ctaaaatagt acataccgga atattttctt ctgccatatc cgtgcaatat tcatcacttc
     7681 taataatgac attcccgata cgtatcgtcc ctacttttgt agagccttcc gcgatagttt
     7741 ttatttgccc tgttatcaat ccatttatga tatctaagag ttcttgaaag gtgatattcg
     7801 ttactccgtg tgcatactgt tgtatgatgg caatcgtttc agcactcagc gatgccatat
     7861 ctgcttctgt tagagtatag gtatttaata acacaaccgg atcctctgta cctccttcaa
     7921 tgatgatagg cggtcttgta cctccggtag ttcctgttcc actgccacta ccgtcatcaa
     7981 caacaggtat attttccgaa ggttgtgtaa gattgtttgt gtttgtagta gaattaagtg
     8041 cagcctccaa tctcttgctg tttagttcca atctcttttt ttgttgtaaa gcttttactt
     8101 cgtttaatac tgtcagcaag tttccatatt gtgatttggc atttgctatt ttatttgctg
     8161 tagaatttgt aaaagaattt gctgtcttat ttgcaaaagt atttgcagtt tgatttccac
     8221 caccattcga ttggtggtat gccataaaca actgattaat atttaacggc aaaagcggag
     8281 tagccgtaaa tgcatctaag aaatccttat gttcatttag tgaagcatcg ccggaagcaa
     8341 ttctttttaa tagtgccaac aatacaattg cctgactgga taagtggata caagatggat
     8401 tcttcaattg gcaggtatgt atgattaagt tatcccacaa tctgaaccaa taagtcagat
     8461 aggtattatt gcttacatcg ggtaaaatgg catttccacc catgaatgag gcaggtgctc
     8521 cggtacttcc caaaacaaaa gttgatatgg aagtgcctaa ttctgcttct accatgtaga
     8581 taaactcttg tttggtcatt gtttctttat gctcgtccac ccatcttact atttttaata
     8641 agtgaggaaa ttgcgaagtg aacgattgtt catccgtaaa taagagtgta taagcatcag
     8701 cggcgttttt tactgcatcg gtagtgctgc cagtaacagt actgtttgca cctatcagcg
     8761 acaaataggt cgtgtagaac aatgcaacgg ctgactcaat atcagacagt tgagtggcac
     8821 ttaggctctg atactgttgc tggatataat gggaatctat ttcctcaata gtatccaatc
     8881 caatttgttt agtgttgaca aaacgaaaga gttcgttcgt tgtctttttt tctaatgttg
     8941 tcattttaaa taatttaatt tgttaaaaaa ttgtttgttt attttttgtg tacgtttgtt
     9001 gagtgtactt ctcactgtta atttgaaaat tggttaattt gaaaatttga aaatgaacgt
     9061 attcgttatt ttcatttttt gcagttttct ctttttatgt tttcatgtgc cccccgtttt
     9121 tttgtggtta tacttcttta aagtttattt tccctctgtt tgtgttacta ttagacataa
     9181 taattcacct tgaatatttg tacttgttaa tatttgctgt ttggttaatt aatattgttt
     9241 tagaaacgct acatgtctac cggtttatag tataatgcgg tctctcgctt tcgtctggct
     9301 cttaagcctt tttcaaactg cgtgtttttg cgcacatgtg tgttttccca aatcaaacga
     9361 atgccctcat cgtccgggtc atcattaatt aaagccaata gcttcgtgcg ccttaaattg
     9421 ccgacgccta tattgtaaat caggcttaac aatgccgata gctggttgtc attcagcacg
     9481 gcttttattt gttgctttag ctggggcaga tattcttttt ctatcttata gtgcaacaac
     9541 tcttcagcct gtgctgccgt aattttatct ccctgtttta cacgggtgtt gtttttgtaa
     9601 tacgtagaac catacccgat agtccacaca tcgcctttgc gcttataggc tgtcaatacc
     9661 aagccttcgc tgttttttat ttcttctaag tttatcatgt gttgtctttt tattggttat
     9721 tcatttttcg tgtacaaatt tgcccggggc gcctgcctgt tggcagacaa gaacatctaa
     9781 gccggccgca gtaaagtctt tatctgtcat atcttgcgtt aagcacgttg ctgttttact
     9841 ttcgcttggg catgagttat ccaccccgag ctttgtactc aatctttttt gctacacctg
     9901 tagccgtttt tttaattcat atacctgagt tcgggttaag aagattatga tggaaaaaat
     9961 agttgatttc tcatattttc ctccaactta acaaggagga ggaattaata ggaggaggtt
    10021 ctatatcaaa aatcaactat ttttaaagtg aaacatatat cccgaacatt ggttaatttg
    10081 cttctttagc tttcttctga ggctaacagt ttcttgctca gcaaagccac ctggtcttcg
    10141 agcagttgca ggcgttcgtt ggtttttctt ctgaagccaa tattttcaaa tcctttgatg
    10201 agcaggttca gtccttcctg tatcaggttg gtgatttctt ccggtgttaa acgattcatg
    10261 tttttcatat ctgtttattt taaaaggtga atagttgatt tatttttatt tgtctgattc
    10321 tttagtagtt atcggcatcg tattgtactc ccgcacaatg gcatttgcaa tcacaactag
    10381 gagtgagaat gttaaacgca ctacgctaaa aacttcgtcg gttaaatgta tgaggttgag
    10441 gttgtccaac actcctcctg ctatataacc acataaggct acgatgctta cccacagcgc
    10501 acggtctttt atgtttgggt tgagatagat ttgtatgcct tgcaaaatga tgagcagcag
    10561 catgagtgat gcaccgatgt acgtacgcat agcgccatct accttagcgg cattaaaggt
    10621 atccaatatg gcctggatgg cggggattaa actaatcaga acggaatatg ctttttgatt
    10681 tttcatga
//
LOCUS       DAOQII010000064        10433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3310_length_10433_cov_5.634419, whole genome shotgun sequence.
ACCESSION   DAOQII010000064 DAOQII010000000
VERSION     DAOQII010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_10433_cov_5.634419"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLF48_04620"
     CDS             complement(<1..317)
                     /locus_tag="PLF48_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48640.1"
                     /translation="MFFRNFYILLISVFLLFACDRKTQKLLKGNDMEAKLQIADNYYN
                     KKKYEKAIPIYEELLTVYKGQKNVEDIYFKYANAHFLNKSYELASFYFRSFYNTYPFS
                     EKA"
     gene            complement(496..1185)
                     /locus_tag="PLF48_04625"
     CDS             complement(496..1185)
                     /locus_tag="PLF48_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48641.1"
                     /translation="MKTRLVLTTIAVIALLQACAPINQPAPRNLPVSGNYDVNILDFG
                     DSINYQAFGKTKLIPIEGDTLCFDLVKGNEYINFMIWVKSASNIQYEYLNYDSLQIDS
                     TKFTLLKKDEHILHLSNARWKPFLYAPGMTIFDMRHDSALIGYDTFSIIGDASIASPS
                     LLFGLSNYQNMEGFLVFRRKYNDTYLYFWLRIKAEPNTTFQLGSLQYNGGYSINVLDG
                     KYQQDSIITGQ"
     gene            complement(1339..2229)
                     /locus_tag="PLF48_04630"
     CDS             complement(1339..2229)
                     /locus_tag="PLF48_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNF48642.1"
                     /translation="MGQEHHHHHAHSAIQKLNSHSLTTAFYVGIVLNSLFVIVELIYG
                     ISQHSLSLISDAGHNLFDVLCLVLALISVWLCKVKTSEQYAFGYKKVSIIVAFFNSAV
                     LLISVFVIFYEAILRLKNPAPLQGKTIAIVALAGILVNGVSALFFLSHKQEDVNVKSA
                     YWHLLADMLLSLAVCIGGVVIYYTGYTKIDALLSMLISVIIAWSSWKLLKESFELLIG
                     KTPASVNKSEISKLILDTRGVKTLKLLRIWAVSTNEFAAHINVELEEGFSTASQQEIK
                     VQICQSLKAKQITYLFWEIC"
     gene            complement(2230..3015)
                     /locus_tag="PLF48_04635"
     CDS             complement(2230..3015)
                     /locus_tag="PLF48_04635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNF48643.1"
                     /translation="MQSSGKLRLLIMLAFIGFSVISYFFKSQVNPVTGEKQRVDLTPQ
                     EEVAMGLQSAPEMAAQFGGLYPDENVQLKVQAIGQKLVNGTEAKNSPYEFHFHVLADP
                     ETINAFALPGGQIFITMGLLKRLQNEDQLAGVLGHEMGHVINRHSSEQMAKQGLMNGI
                     VQGVAMGSGDMNTAQIASYVSNVIGLKYGRDDELESDKFGIKYMYQTGYQPEQMLGVM
                     EILKQASGGGNQDDFMSTHPSPENREIKIKEEIAQLKSKSLTQ"
     gene            complement(3022..3438)
                     /locus_tag="PLF48_04640"
     CDS             complement(3022..3438)
                     /locus_tag="PLF48_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNF48644.1"
                     /translation="MQLNPEFTPLIRLVVATVIGVMIGLERQKNGKIAGIRTFGTLAL
                     GTAAICIFALKMQGMANGMEWVIIASIIIAIGFISARMFIIDSGNGVQDFSNITAIWC
                     TAAISILISFGFYTLGGITAFLLLLIFLLKDVYEKK"
     gene            complement(3523..4353)
                     /locus_tag="PLF48_04645"
     CDS             complement(3523..4353)
                     /locus_tag="PLF48_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HNF48645.1"
                     /translation="MIKTRNLHIYLFSLLFTLVLGLVFFYLNHALDEAIWSGMQISKS
                     ALTAEYCELNRTDHLFHQPVNTYSNLFYFFLGSIVVGLSIKSTSPSNRNSLLQFPALM
                     AFCGLSLIYLCFGSSFFHASMTWSGQRVDMNATYAVCLSCLAISLYYYKIQAFSTGLK
                     TSYIIFLFLLVLFFVYLHLLISSLVLLPTLIILILFFTVINYNRNKSALNIQYAFLSL
                     FFVVGAFILRTLDVMKIVCNPTSVFQGHALWHIFTGLSAFFLFWFYYSEKISVQDKTQ
                     "
     gene            4511..5026
                     /locus_tag="PLF48_04650"
     CDS             4511..5026
                     /locus_tag="PLF48_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNF48646.1"
                     /translation="MKTILITIISLFSIKSCFNSNSFKLIVAMEDASDLKAGNKVKCK
                     GVEVGEVNNIQIVGNKVYAEIGLNNDFLPVKGTTAQVNFENLLGGKSLVLYPSASEEL
                     LASGDTINATKNESMGIIEDIMKKNIPIDSIMGNFNLDSIGSELLKDTASINKMKDAA
                     EKIFKLKDLIK"
     gene            complement(5044..5634)
                     /locus_tag="PLF48_04655"
     CDS             complement(5044..5634)
                     /locus_tag="PLF48_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48647.1"
                     /translation="MQNPEVDTVNTVVHEKDQKGNNNLIYVGYAPSFHLGNGAKNVFH
                     GIELNYERKIIQRLSISFTQGFYSTSKKDYLWYENLKLFSVKRTDYYFSTYLTMNAIL
                     YYNKIYMLKLGLGPSLYYHDVIHLTSNDPSVDFSKNYFDKGVLGGLHINLQNDFYIKK
                     SLYLGFKFQTHFIFPKKNSGDKIIIMRPGLNLGYSF"
     gene            5960..7927
                     /gene="gyrB"
                     /locus_tag="PLF48_04660"
     CDS             5960..7927
                     /gene="gyrB"
                     /locus_tag="PLF48_04660"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNF48648.1"
                     /translation="MSEEIIEKQQKAQHYGADNITVLEGLEAVRKRPAMYIGDINVRG
                     LHHLVYEVVDNSIDEALAGYCKNIFVNIHEDNSISVEDDGRGIPVDMHPKEGRSALEV
                     VLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSSHLTATVNRDGKVYQQEFEIGAPL
                     YSVKEVGTTDKRGTIIHFKPDTTIFTVSEYNYDTLAARMRELAFLNRGIHITITDMRN
                     KDENGTPFAEHFYSEGGLREFVSYLDKNRQALIPEVIYMEGTRENVVVECAFQYNDSF
                     IENIHSYVNNINTIEGGTHVVGFRRALTRVFKNWGTKNDLFKNLKVEISGEDFREGIT
                     CVVSVKVPEPLFEGQTKTKLGNSEVDGIVAGIVGEMLEYYLEEHPKEAKTIINKVVLA
                     ATARHAARKARELVQRKSGLGGSGLPGKLSDCSDKDARACEIFLVEGDSAGGTAKQGR
                     NRAFQAILPLRGKILNVEKAMEHKIYENEEIKNMFQALGVSKGVEGDEKALNLTKLRY
                     HKIIIMCDADVDGSHITTLILTFFFRHMKELVEQGHIYIASPPLYLVKKGNKQKYAWN
                     EESRKAAMKEIGGEGKEDSVHVQRYKGLGEMNAEQLWETTMNPETRTLKQVTIESAAD
                     ADYIFSMLMGDEVPPRREFIETHAKYARIDV"
     gene            8241..9374
                     /locus_tag="PLF48_04665"
     CDS             8241..9374
                     /locus_tag="PLF48_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF48649.1"
                     /translation="MKYILYLLFICLLFTSCSCNKNDNNTSQPNYFYYSSEAEFIANN
                     SGLSDTAILGKKIFFDKNLSEPVGISCATCHSPMNGFSDSDHEQFSSGVNGHKGMRNA
                     NAISYMVFAPNRSPQLVRGITEMTGGFFWDGKAEFLNQQALFPMINPDEMNNPNFASI
                     ANKISHSSYYNDMVSVFGADNLVDSQTIVFAAITALQSFEKSFQLNPFTSKYDFYLRG
                     KTSLTAQELRGLNIFNDTSKAKCSLCHRSEPTVYASNHNILFTDYSYENLGLPKAPTQ
                     LGLPTDSGLGKFEWDDPLEVGRFKTPTLRNIALTAPYMHNGIFTTLEEVLDFYNTRDI
                     NSAFVPEYSVTMNHEDLGNLHLTDQEKQDIIAFLKTLSDGFKP"
     gene            complement(9377..10309)
                     /locus_tag="PLF48_04670"
     CDS             complement(9377..10309)
                     /locus_tag="PLF48_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNF48650.1"
                     /translation="MSLLVVGTVAFDAIETPFGKTDKITGGSGNYICQSAGHFTDNMQ
                     LVSVIGDDFDQSELQYLQSIGTDLEGVQVKKGEKSFFWSGKYHMDLNQRDTLVTDLNV
                     LADFDPILPEKYKTAEFLLVGNIDPTLQAKVISQMHERPKLIALDTMNFWMQIALDAL
                     HNVLKMVDLLIVNEEEARQLSNEYSLVKAANKIRAMGPKYLIIKKGEHGALLFHGNRV
                     FFAPALPLEEVFDPTGAGDTFAGGFMGYLSKTEDVSFENMKRAVIYGSAMASFCVEKF
                     GPTRLKEITRGDIEERVNVFVELVDFDIILNEEA"
BASE COUNT         3313 a         1943 c         2002 g         3175 t
ORIGIN      
        1 tctgctttct cgctgaaggg ataagtgtta taaaaactgc ggaagtaaaa agaggccaac
       61 tcgtagcttt tgttgagaaa atgcgcattg gcatatttga aataaatatc ctccacattt
      121 ttctgtcctt tatacacggt taacagctcc tcgtaaatgg gaattgcttt ttcgtatttc
      181 ttcttattgt agtagttatc cgcaatttgc agtttggctt ccatgtcatt gcccttaagc
      241 aatttctgcg ttttacggtc gcaggcaaac agcaagaata cagagatgag aagaatataa
      301 aaattcctga aaaacataag cctgcaaaaa taggaaataa atgtggaaaa ataagggttt
      361 tgtgttggaa aaggcttaaa aatgctgatt tttaggctca tacgtgttaa ataaatttaa
      421 acacaccccg aaagtgaaaa ataaagaaag aggccggata ttgttcagcc tctttttggg
      481 gatagattta aagacttatt gtccggtaat gatgctgtcc tgctgatatt tgccgtctaa
      541 aacattgata gaatacccac cgttatattg caaactccca agctgaaatg ttgtatttgg
      601 ttctgctttt atccgtaacc aaaaatatag ataagtgtcg ttatattttc ttctaaatac
      661 aagaaatcct tccatgtttt ggtaattaga caatccaaat aataggctag gcgaagcaat
      721 ggatgcatcg cctataatcg aaaaagtatc atacccaatc agtgcagaat cgtgtctcat
      781 gtcgaagata gtcatgcccg gggcatacaa aaatggtttc catcttgcat ttgacaggtg
      841 tagtatatgc tcatcttttt tcaataaggt aaatttcgta gaatctatct gcaaggagtc
      901 ataatttaag tattcatatt ggatattact tgctgatttc acccaaatca taaaattgat
      961 atactcattt cccttcacca agtcgaaaca taatgtgtct ccttctatgg gtattaattt
     1021 tgttttgccg aatgcctgat aattaattga atcaccgaaa tccaatatgt ttacatcata
     1081 atttccactt acaggtagat ttcgaggtgc aggttggtta atgggtgcgc aggcttgtaa
     1141 caatgcaata acagcaatag ttgtaagtac gagtcttgtt ttcattagtg tctcatttaa
     1201 ttacttcaaa aataaataat attttgataa tatttccaga taaataatcc ttattgttga
     1261 aagaatatta tgaaaatatt ttacaaagac atgaaataca atacgttgtg atttatactg
     1321 tatggaagat tgctctattc aacagatttc ccaaaaaaga taggtgattt gtttagcttt
     1381 cagcgactgg cagatttgaa cttttatttc ttgttgagat gctgtagaaa atccttcttc
     1441 tagttctaca ttaatatgtg cggcaaattc attagtactt acggcccata ttcttaatag
     1501 tttcagtgtt tttacgcctc ttgtatcgag aatcagtttg cttatttcgc ttttatttac
     1561 agatgcaggt gtttttccaa tgagcaattc aaagctttct ttaagcagtt tccagctgct
     1621 ccatgcaatg attaccgaaa tcaacatact gagtaatgca tcaatcttgg tatatccggt
     1681 atagtaaata accacacctc caatacatac tgccaaagat agcagcatat ctgccagcaa
     1741 gtgccagtaa gcagatttca cgtttacatc ttcttgttta tgtgataaaa agaatagggc
     1801 ggaaactcca ttaacaagta tgccggctaa ggctactatg gcaatcgttt tgccttgcaa
     1861 aggtgcagga ttttttaagc gcaaaatggc ttcataaaag atgacaaaaa cactaatcaa
     1921 taataccgca gaattaaaaa atgccacgat tatgcttact tttttatagc cgaatgcgta
     1981 ttgttctgat gttttcactt tgcacagcca aacggagata agagccaaaa ccaaacataa
     2041 tacatcaaaa agattatgac ctgcatcgga tattaaagac aaggaatgtt gtgaaatacc
     2101 ataaatcagc tctacaatta caaataatga attgagcaca atgcccacat aaaaggctgt
     2161 ggttaacgaa tggctgttca gtttttgtat cgcagaatgt gcgtggtgat ggtggtgttc
     2221 ttgccccatt tattgcgtta agcttttcga ttttaactgg gcaatttctt cctttatttt
     2281 tatttctctg ttttccgggc tggggtgagt gctcataaaa tcatcctgat ttcctccacc
     2341 agaggcttgt tttaaaattt ccatcacacc aagcatttgt tccggttgat atccggtttg
     2401 atacatgtat ttgatgccaa acttatcgct ctccaactca tcatctcttc catattttag
     2461 tccaattaca ttacttacat aagaggctat ttgtgccgta ttcatatcgc cgctgcccat
     2521 ggctacacct tgtacaatgc cattcatcag tccttgtttt gccatctgct cagaagagtg
     2581 tctgttgatg acatgtccca tttcatggcc taatactccc gctagttggt cttcattttg
     2641 caggcgtttt agcaagccca tggtgataaa tatttgtccg cccggcaaag caaatgcatt
     2701 aattgtttcg gggtcagcca acacgtgaaa atgaaattca taaggtgaat tttttgcttc
     2761 ggtgccattc accagttttt gcccgatggc ttgcacttta agctgtacat tctcatccgg
     2821 gtacagacca ccaaattgtg cggccatctc gggtgctgac tgcaagccca tggctacttc
     2881 ttcctgcggt gttaaatcca ccctttgctt ttcgccggtt accggattga cttgagattt
     2941 gaaaaaatat gaaattacgg aaaacccgat aaaggctaac ataattaata aacgaagttt
     3001 gccactgctt tgcataatag tttatttttt ttcgtaaacg tcttttaata aaaatataag
     3061 taataacaag aatgccgtaa tgccacccaa tgtataaaag ccgaaagaaa taagtatgga
     3121 aattgctgct gtacaccaaa tggcggtgat attggaaaaa tcttgcacgc cgtttccgct
     3181 gtcaataata aacatgcgag ccgaaataaa gccgatggca ataataatag atgcgataat
     3241 tacccattcc ataccatttg ccatcccctg catttttaat gcaaaaatac agatagccgc
     3301 agtgccgaga gccagtgtgc caaaggtgcg gataccggct attttgccgt ttttctgacg
     3361 ttccaaacca atcattacgc caattacagt ggctacgact aatctaatca atggagtaaa
     3421 ttccgggttt aactgcatat tttaattatt taataaataa aggtagtcat tttttatgaa
     3481 atgaagtgaa cttgggtttc ggctcttgat gcatcaatct tactactgag ttttatcctg
     3541 cacacttatt ttttccgaat agtaaaacca aaataaaaag aaagcactca gtcctgtaaa
     3601 tatatgccaa agtgcatgtc cttgaaacac agaagttggg ttgcatacaa ttttcattac
     3661 atccagcgtg cgcagaataa atgcaccaac aacaaaaaac agacttaaaa atgcatactg
     3721 aatatttaat gccgacttgt ttctattgta atttattacc gtaaaaaaca aaatcaaaat
     3781 aattaaagtt ggcaacaaaa ccaggcttga aatcagcaaa tgaagataca caaagaaaag
     3841 aacgagcaaa aaaaggaaga tgatatagct tgtttttaac cctgtactga atgcttgtat
     3901 tttgtaatag taaagactaa ttgccaaaca cgataaacaa acagcataag ttgcattcat
     3961 atccacacgt tgtcctgacc aggtcatgga ggcatgaaaa aacgaactgc caaaacacaa
     4021 ataaatcaaa cttaatccgc aaaaagccat caaagcagga aattgcagta aactgttcct
     4081 gttgcttgga cttgtgcttt ttatactcag accaactaca atactgccca ggaaaaaata
     4141 aaataaatta gagtaggtat ttaccggctg atgaaataag tggtcggttc tgtttagctc
     4201 acaatattct gcggtaagcg cagacttgct gatttgcata cccgaccaga ttgcttcatc
     4261 aagcgcatgg tttaaataaa agaaaaccaa acccaagaca agggtaaaca ataatgagaa
     4321 taagtatatg tgtaagtttc gtgtcttaat catcttataa aaatgctgta cctgttttaa
     4381 ttctatttgg aaagtcctct tgtaaaaata agaagtttga cgaactatct ggagaagtct
     4441 ggacagtcat tcatgattat ttttgttagg agatgagtag gaactttatt atcttgtcct
     4501 gaatatgatt atgaaaacga ttctgattac aattatttct cttttttcca taaaatcttg
     4561 cttcaacagc aattctttta agctcattgt tgccatggaa gatgcatctg atttaaaagc
     4621 cggcaacaag gtaaagtgca aaggcgtaga agtgggagaa gtaaacaata ttcaaattgt
     4681 gggcaataaa gtctatgcag aaatcggcct aaataatgac tttcttccgg tgaaaggtac
     4741 aaccgcacag gtaaattttg agaacctgct gggaggaaaa agtctggtgt tatatccttc
     4801 ggcatctgaa gaactgcttg cctccggcga tacaataaat gccacaaaaa atgaaagtat
     4861 gggtattatt gaggatatta tgaaaaaaaa tattcccata gattctataa tgggaaattt
     4921 taatctcgac tctatcggca gtgaactatt aaaagacacg gccagtatca ataaaatgaa
     4981 agatgccgct gaaaagatat ttaagctgaa ggacttgata aaataaatag gctggtggtt
     5041 tagttaaaaa gaatagccca aattcaaacc agggcgcata ataattattt tatctcctga
     5101 gtttttcttt ggaaaaataa aatgagtttg aaacttaaaa cccaaataaa gacttttctt
     5161 gatgtaaaaa tcattttgca ggttaatatg taatcctcct aaaacacctt tatcaaaata
     5221 atttttacta aagtccacag atggatcgtt acttgttaga tgtataacat catgataata
     5281 caacgatgga ccaagcccaa gttttaacat ataaattttg ttataataca aaatagcgtt
     5341 catggttagg taagtactaa aataataatc cgtacgtttt acggaaaaca atttcaaatt
     5401 ttcataccag agataatctt tttttgaagt agaataaaaa ccttgagtaa atgaaatgct
     5461 caggcgttga attatctttc gttcatagtt taattctatg ccgtgaaaca cattttttgc
     5521 accatttcct aaatgaaatg aaggtgcata tccaacatat attaaattgt tattcccctt
     5581 ttggtctttt tcatgaacta cagtatttac cgtatccact tccggatttt gcaagatatc
     5641 ctgtttccct attgccatca acctgaatga aaaaatgaag agtaagacga agatgttttt
     5701 cataagcttt ttagagtata tagttgaaat atggaataaa tatatcaaaa aaaaccaaca
     5761 aaataaaatt atatctgaca tgcctttgtt ttaatcctta ttaacgcttt tttgtggata
     5821 atctgccggc attttatccc attttaaggc tattatttcg tatcttcgtt caccttttta
     5881 aactcgggta gttataccgt tatctggcaa tttatttatc ctgccaataa cttatcgcta
     5941 ctcactatac actacaaaaa tgagcgaaga aataatagaa aaacaacaaa aggcacagca
     6001 ctatggtgcc gacaatatta cggtactgga agggctggaa gccgtgcgca aacgccctgc
     6061 gatgtatatc ggcgatatta acgtgcgagg cttgcaccac ttggtgtacg aagtagtaga
     6121 taactcgata gatgaggcac ttgcaggcta ttgtaaaaat atttttgtaa atatacacga
     6181 ggataactct atttctgtag aagacgacgg caggggtatt ccggtggata tgcatcccaa
     6241 agagggcaga agtgccttag aggtggtact tacggtattg catgcaggcg gtaagttcga
     6301 taaagactcg tataaagtat ccggtggttt gcacggcgtg ggggtttcgt gcgtaaatgc
     6361 gctttcatcg cacctgacgg caaccgtaaa ccgcgatggc aaggtttatc agcaggagtt
     6421 tgaaattggt gctcccttat attccgtaaa agaggtaggc actaccgata agcgaggcac
     6481 cattatccat ttcaaaccgg atacgacgat ttttacagtt tctgaataca actatgacac
     6541 gttggcggca cgtatgcgtg agctggcatt cctcaacaga ggcatccata tcaccattac
     6601 cgatatgcgc aataaagatg aaaacggaac accttttgcc gagcattttt attccgaagg
     6661 cgggcttaga gagtttgttt cttatttgga taaaaatcgc caggcactga ttccggaagt
     6721 aatttacatg gaaggaacca gagaaaacgt tgtggtggag tgtgccttcc agtacaatga
     6781 ttcgtttatc gaaaacatcc actcttacgt caacaatatc aataccattg aaggcggtac
     6841 gcatgttgta ggctttagaa gagccttaac gcgtgtattc aaaaactggg gcactaaaaa
     6901 cgatttattt aaaaatctga aagtagaaat atccggcgag gattttcgtg aggggattac
     6961 ctgcgtggtg tctgtaaaag ttccggaacc tttatttgaa ggacaaacca agaccaaatt
     7021 gggcaactcg gaagtggacg gcattgtagc gggcatcgta ggcgagatgc tggagtatta
     7081 cttagaagaa catccgaagg aagcaaaaac cattatcaat aaagtggtgt tggcggctac
     7141 agcccgacat gcagcccgca aggctcgtga actggtacag cgcaaaagtg gcttaggcgg
     7201 ctcgggtttg ccgggcaagc tttcggattg ctcggataaa gatgctcggg cttgcgaaat
     7261 ttttctggta gagggcgact cggcgggtgg aacggctaaa caaggtcgaa accgtgcttt
     7321 tcaggcgata ttgcctttgc gcggtaaaat tttgaacgtg gagaaagcga tggagcataa
     7381 gatttacgaa aacgaggaaa tcaaaaatat gtttcaggcg ctgggtgtga gcaaaggtgt
     7441 ggaaggcgat gaaaaagccc tgaacttaac caaattgcgt tatcacaaaa tcatcattat
     7501 gtgtgatgcc gatgtggacg gcagccatat cactacgcta atcttaacct ttttcttccg
     7561 ccacatgaaa gagctggtgg agcaaggaca tatttatatt gcttcgccgc ctttatattt
     7621 ggtaaaaaaa ggcaataaac aaaaatacgc atggaatgaa gagtcgcgca aggcagccat
     7681 gaaagaaata ggcggtgagg gcaaagaaga cagtgtgcac gtgcagcgct ataaaggttt
     7741 gggggagatg aatgccgaac aattatggga aaccaccatg aatccggaaa cgcgtacatt
     7801 gaaacaagta accatagaaa gtgcggcaga tgccgattat attttcagta tgctgatggg
     7861 cgacgaagtg cctccgcgcc gagagtttat agaaacccac gcaaagtatg cgaggataga
     7921 tgtgtagcct ttattaataa aaagctttta aattcaagaa gaaacacaca aattctttct
     7981 tggtataatt gtcaaatgta agattacttc ttgcatctta taattttata tgtataacta
     8041 tttacgttga tttggaatca caataaatgt attctttata accataagct tccaagctaa
     8101 tatccaaagc tatttcagtt tgttttacaa aatttatgtc cataattccc aattatgact
     8161 atctgccaaa tatttactaa atagtgctga ttattttgtt aattaaaagg agattgcata
     8221 aagaatgatt aattttaaac atgaaataca ttctttactt gttgttcatt tgtctcttat
     8281 tcacttcgtg ttcctgcaat aaaaacgaca ataacacctc tcagcctaat tatttttatt
     8341 actcgtcaga agctgaattt attgccaata attccggact ttcagacact gctattttag
     8401 gcaagaaaat atttttcgat aaaaacctat ctgagccagt gggcatcagt tgtgccactt
     8461 gccactctcc aatgaatggt ttttctgatt ctgaccatga acaattttct tccggggtga
     8521 atggtcataa aggaatgcgc aatgccaatg ctatcagtta tatggtattt gctcccaatc
     8581 gttctcctca gcttgtgagg ggaattacag aaatgaccgg cggttttttc tgggatggta
     8641 aagctgagtt tttaaatcag caagcgttgt ttcctatgat taatcctgac gagatgaaca
     8701 atccgaattt tgcttctatt gctaataaaa taagccattc atcgtattat aacgatatgg
     8761 tttccgtatt tggtgcagat aatctcgttg acagccaaac tattgttttt gcagctatta
     8821 cagcgttaca aagttttgaa aaaagttttc agctgaatcc ctttacaagc aaatacgatt
     8881 tttatctaag aggaaaaaca agcttaacgg cacaggagct tagggggtta aatatattta
     8941 atgacacatc aaaagcaaag tgcagtttat gccacagaag tgaacctact gtttatgcct
     9001 ccaatcacaa tattttattt actgattaca gttatgaaaa tttaggctta ccaaaagccc
     9061 ctacgcaact gggcttgcca acagatagcg gtttgggaaa atttgaatgg gacgacccgt
     9121 tggaggttgg acggtttaag acacctacat tgcgcaatat tgctttgact gcaccttata
     9181 tgcacaacgg catttttacg acattagagg aagttctgga tttttacaat acccgtgata
     9241 taaattccgc ttttgtgccg gaatattctg ttaccatgaa tcatgaagat ttgggaaatc
     9301 tgcatcttac agatcaggaa aagcaagata ttattgcctt cctgaaaaca ttaagtgatg
     9361 gctttaagcc gtaatcttat gcttcttcgt tcaggataat atcgaagtca accaactcta
     9421 caaacacatt tacacgctct tcaatatctc cgcgggttat ttcttttaaa cgggtaggtc
     9481 cgaatttttc tacacaaaag cttgccattg ctgaaccgta aataacggca cgtttcatgt
     9541 tttcaaatga aacatcttcc gttttagaca gataacccat aaaccctccg gcaaatgtat
     9601 cacctgctcc tgttgggtca aaaacttctt ccaatggcag agccggagcg aagaaaactc
     9661 tgttaccatg aaacaataat gcaccgtgtt cacctttttt aataatcagg tatttaggac
     9721 ccattgcacg gattttgttc gccgctttta ccaaggaata ttcattagat aattgccggg
     9781 cttcttcttc attgactatc agcaagtcca ccatttttag tacattatgc aaagcatcta
     9841 gtgctatttg catccagaag ttcatggtat ccaatgcaat caacttaggt cgttcatgca
     9901 tttggctaat aactttagct tgtaaggtag ggtcgatgtt gcctaccaac aagaattctg
     9961 ctgttttata tttttccggt aaaatgggat cgaagtcagc caaaacattt aagtcggtta
    10021 caagcgtgtc gcgctggttt aagtccatgt gatatttacc actccagaaa aaagattttt
    10081 cacctttttt tacttgcaca ccctcaaggt cagttccaat agattgtaaa tattgaagtt
    10141 ctgattggtc gaaatcatca cctatcacgc tcactaactg catgttatcc gtaaaatgcc
    10201 cggctgactg acaaatataa tttccggaac cgcctgtgat tttatctgtt tttccgaaag
    10261 gtgtttcaat ggcatcaaat gcaactgtgc cgacaactaa aagactcatg taaaaaaatt
    10321 tgtacaaata ttgcatattt attttgaata gcctattttt gcagtccaaa aaacagaatc
    10381 ttccttagct cagttggtta gagcatctga ctgttaatca gagggtcgct ggt
//
LOCUS       DAOQII010000065        10358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3354_length_10358_cov_4.699214, whole genome shotgun sequence.
ACCESSION   DAOQII010000065 DAOQII010000000
VERSION     DAOQII010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_10358_cov_4.699214"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLF48_04675"
     CDS             complement(<1..432)
                     /locus_tag="PLF48_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48651.1"
                     /translation="MNLEIPKKFNGIAQVAVLAAKNIFRPVSRKYDMAEHAYPVELDL
                     LAAGLNGLNDGSGGAVGGASGGKNDTDAASTVRNGANMANCINIQAMCYGDVGLLLSI
                     PKQGLGNAAISAVANAEQRARFAEVWAAMAITEPNCGSDSSA"
     gene            complement(442..1770)
                     /locus_tag="PLF48_04680"
     CDS             complement(442..1770)
                     /locus_tag="PLF48_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF48652.1"
                     /translation="MGKEKKRSFWQNLPLDEYFTTYNKLLDSAIGKRLNLRKFSENFI
                     YQGTKNGMRASAAIGKIFEPSDKSASRFTTKSTSDYFDLNYTEEQLLVQESVKEFALK
                     LRVAAQEQDENKHLTDDIKQAYQELQLHYLQIPESLGGFGNEKSTITQMFILENLAYG
                     DLGQAYALYASNSMLNALVQWGNEAQQAALIPELLNEVPKYAGIAINEAELLFSPFEL
                     KTSANEDGDVYVLNGKKSLIPFAEKCDYFLVAANTNDGDQQLFLVDAALQGVSIKKKE
                     AMGLKTAEFGDLHLSNVRIDKSAKLGGEDGINYPEFIQYAKLGWCALAVGCCQAVLDY
                     VINYSNERFAFGEPISNRQSVAFMIADMKIELDSMRILTQRAAAQAEHGQDFSKATYL
                     AHVFCGDKAMQIGTNGVQLLGGHGYIKDFPVERWYRDLRAIAILFNNMHL"
     gene            1975..2589
                     /locus_tag="PLF48_04685"
     CDS             1975..2589
                     /locus_tag="PLF48_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48653.1"
                     /translation="MLKYIFIALLKNYTDNESLINKLWDEIEQHYTDKKRHYHTLEHL
                     NKLFDHLSEVKNEIQHWHILLFSLFYHDIIYNTLKSDNEEKSAALAVKRLTQLGIADS
                     EIRLCSHQILATKSHQLSDNPDTNYFTDADLSILGQNWETYSNYMQQVRKEYAIYPDF
                     IYKKGRKKVVHHFLAMENIYKTTYFQVKLEQQANQNLQKELSIL"
     gene            complement(2605..3036)
                     /locus_tag="PLF48_04690"
     CDS             complement(2605..3036)
                     /locus_tag="PLF48_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF48654.1"
                     /translation="MERIKINLPEHFIYREFFSIENADINEAEHMGNERILIFANGAR
                     QRFFKFIKFPELDLENNCGSIIANHSIVYKAEGFLGDKIECEVGFTNCTNYSFDLVLH
                     FIKNGFTTMAIVRTGIVYFDYKKKEVIEMPEIIKIFYPENA"
     gene            complement(3044..4051)
                     /gene="tsaD"
                     /locus_tag="PLF48_04695"
     CDS             complement(3044..4051)
                     /gene="tsaD"
                     /locus_tag="PLF48_04695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNF48655.1"
                     /translation="MPEIVLLAIESSCDDTSAAVCVDGKIISNLTANQKIHEQYGGVV
                     PELASREHLKNIVPVIQQALQTANVYLQDLSAIAYTQGPGLLGSLLVGGTFAKSMAQC
                     LQIPIIAIHHMHAHILAHFIDEPKPKFPFLCLTVSGGHTQLVRVNSYNDMQILGQTLD
                     DAVGEAFDKTAKLLHFPYPGGPLIDKYARLGNPDAFVFNEPQIEGLNFSFSGLKTNIL
                     YFLQKQEKQNPDFISDNLNDICASVQKSIVTILLKKLKRAVNETGIKEVAIAGGVSAN
                     SGLRNALQQMGEKQKWNVYIPAFQYCTDNAGMIAIAAYYKYIDKQFSTLDAPIQARLV
                     F"
     gene            4094..8926
                     /locus_tag="PLF48_04700"
     CDS             4094..8926
                     /locus_tag="PLF48_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48656.1"
                     /translation="MGKFFKYVFRTLLILCAILLISFITLYYLIQQPKYQTLAVRKVS
                     EILSKQLGSEVKVDSVNLKFISTIQIHKVYLSEKNKPNDTILFINRLDASLMLGKTLM
                     EQISSLQSAKYYVDDVNIDGLKFHGYRGLNDSIYNFQFLLDNFKSKNNKPTPKKAKKP
                     IELRLNELTLTNGNLVLDDIYKDQRFDIRYTKVDINVRQLDINHLKIDAKKVVLEDPY
                     FCMTDYNNIPSKPKIKKPTLGFKIQELGNKLNINVDKLTLVNGQHRLDFQHKDQKAGN
                     FLISQMNIQNIVLNMDNYVWDSTGMTGKLTNLSANADNQIKLKNLSGLIHLDNGGIYL
                     DNADLALNNSILKGNLSLQFLTGHWNSLSNFQDEVMLKADLKETKTNGHDVALFAPKV
                     QKYIPPNTRLEGLIRGRLSNLKVENLKLTAADNTVIDISGNIKGLPKIQQTLFDLNIH
                     QLKTNASELKQILTFVKLPKQLDNAGNISFKGSYFGFINDFVAKGNLTTTNLGNLTTD
                     LRMRFPKGKSPEYSGNVTANGLNLAELTGNKKLLGTVDLDIAANGKGFNAKELSTDLK
                     GTIRNFYLNGFVFDKILVDGFLDKKKFKGKAFFDDKCFLVDFNGMVDFNNELPKFDFT
                     TSIKNADLNKLHLTKDTLLVSLDGEVHGSGKSLDNLIAAGNFSNMMIQNQKDILVLSD
                     VAMNVKNSPTFRDYNIISDQFNAHVNGNFNPLNIVPSMKVFLSQYSKLIKPTAKDLKL
                     AKPQQLNADIKLKSDFGIFKVFVPKVKYLSEVEIKSNINTTDKKLTLTANLDSAVYDK
                     LVMNKIILDAETRENNLVADIVLDKLQSGKTIVNDIGVNLTSSAENLLTNISISNDTA
                     KNSVRLTSSLDFRGDSVIAKILDSKLKLNNKIWTVAPNNQLTIVDSIFLTQNIILLQD
                     DQKISIINGRNSLEDAKINIENLNLTDIVQLVDTSHILQNGFLSGNVSLKNILTKPLV
                     NADLNINELQVAGYKVKIVGLSGIYGRDGKSIVEVNGTLDDKDYQLAFDGTYDMQIKG
                     KEKFDVTADIEKLNISFLETILKKELLVPRAFVKGQVNVSGNLKNPILNGTAEIIDTA
                     ELKFRMLGTTFKLYKEKIKLTREGFDFGEMTVYDNFGNTALLSGKLTHTGFKNFGVEN
                     VVFSAPIQYNFMNTTYEDNQDFYGKVFAKGDVFMNGSFNDLSIKIPDLTTLKNSEFNL
                     PVSDKAADKGYAWIKFIDPKDTSKRVAYKTKLSGINIDLNVNATKDAVVNLILDPSSN
                     DKITGRGYGNINVVMDKAGNFNIYNTYYLTEGKYDFTFQGILNKTFVIKDGSKIIFNG
                     KPDDAELDIKALYNVKSASVRNLFDTITQDERNIRTKSFPIDLNLNITGSLKKTKVSF
                     DISATPGTASANSDLLGRKLAEISNNENDVNNNAATLLLFNSFFPTGTSSDQRFSGAS
                     SSVTDLLLGQISNLISQGLGKLIKGASLDLVLNELETQERNFGFTYKQELIGGRLILT
                     IGGNVNFGNSSTINATSLSQQNSNNAIAGDFMIEYLVTTDGRIRLKAYGKASNYDIFG
                     QDRFRTGGAISFQKDFDDFRDLFKIQNKRNKPIPVLDSLPPAPPPADTGKLKETANIK
                     "
     gene            8993..10300
                     /gene="thrC"
                     /locus_tag="PLF48_04705"
     CDS             8993..10300
                     /gene="thrC"
                     /locus_tag="PLF48_04705"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNF48657.1"
                     /translation="MKLYSTNKISPDVDLKTAVLQGLPPDNGLYMPYEIPILPEGFWK
                     NLSSYSFSEMAFHIAKNLFGNDISDKDLNQIIHQAFTFEAPLIEVGDSYILELFHGPT
                     LAFKDFGARFMGQLSGYFLRGNDKQVDILVATSGDTGSAVAHGFYKVPGVHITILYPK
                     GKVSEIQEKQFTTLGENITAIEIDGTFDDCQALVKQAFLDKELNEKLTLSSANSINIA
                     RLIPQSFYYIDAYRQLDDKSLPLYFSVPSGNFGNLTAGLFAKKMGLPVTKFIASNNAN
                     AVFTDYVHSGNFKPKPSVHTLSNAMDVGNPSNFARMMDIYQQRLDDLKNDITAYSFND
                     EQTQKCITDYALNYDYILCPHTAVGVLGMNQFIDDEDLNPLYINAITLSTAHPIKFGE
                     IVEPLIQDKIDIPERLKEIIKGEKVSLSQKATFEEFKSYLLNR"
BASE COUNT         3562 a         1915 c         1745 g         3136 t
ORIGIN      
        1 agctgaagaa tctgagccac agttaggttc cgtaatagcc attgccgccc acacctctgc
       61 aaatcgtgca cgttgctcgg cattggcaac tgcgctgatg gctgcattgc ccagtccttg
      121 tttgggaatg gataacaaaa gtcctacatc accatagcac atggcttgta tattgatgca
      181 atttgccatg ttagcaccat tgcgtacagt gctggctgca tccgtatcat ttttaccgcc
      241 tgatgcgcct ccaacagccc caccggagcc atcatttagc ccgtttaatc cggcagctaa
      301 taaatccagt tcaacggggt atgcatgttc tgccatatcg tattttcggg atacagggcg
      361 aaatatattt ttggctgcca atactgcaac ttgagcaatg ccattaaatt tttttggaat
      421 ttctaaattc atacttctgt tttataaatg catattatta aatagaatag cgatggctcg
      481 taagtcgcga taccagcgtt ctacaggaaa atctttaatg taaccatgac cacctaataa
      541 ctgaacacca tttgtgccta tttgcatagc tttatcacca caaaaaacat gagctaaata
      601 agttgccttt gaaaaatctt gcccgtgttc agcttgtgct gcagcccgtt gcgttaaaat
      661 acgcatactg tcaagctcaa ttttcatatc agcaatcata aaagctacag attgcctgtt
      721 gctgatgggt tctccaaatg caaaacgctc attgctgtaa ttaattacat aatccaacac
      781 tgcctgacaa caacccacgg ctaaagcaca ccaacctaat tttgcatatt gtataaattc
      841 cggatagttg atgccatcct ctccacccaa ttttgcactt ttatctattc ttacattcga
      901 caagtgtaag tcgccaaatt cagcagtctt taaacccata gcttccttct tttttattga
      961 aacaccttgt aaggcggcat ctaccaaaaa gagttgttga tcgccatcat ttgtatttgc
     1021 tgccactaaa aaataatcgc atttttctgc aaacggaatt agtgattttt ttccattcaa
     1081 cacataaaca tctccatcct cattggcact ggtttttaat tcaaatggag aaaaaagaag
     1141 ttcagcttca ttaatagcaa tacctgcata tttcggaact tcatttagca gttcaggtat
     1201 taatgccgct tgctgagctt catttcccca ctgtaccaag gcatttagca tactattgga
     1261 agcatacaag gcataagcct gacctaaatc gccataagcc aaattttcga gtatgaacat
     1321 ttgggtaatc gtggatttct cattgccgaa acctcctaaa gattcaggta tttgtaaata
     1381 gtgcaactgc aattcctgat aagcctgttt gatatcgtct gtcaaatgct tattctcatc
     1441 ttgctcttgt gctgcaacac gcaatttaag ggcaaattct tttactgatt cctgaacaag
     1501 caattgctcc tcggtatagt ttaaatcaaa atagtcggaa gtggatttag tagtaaaacg
     1561 agaggcggac ttatcggaag gttcaaaaat tttgccaata gcagcagatg ctctcatccc
     1621 attttttgta ccctgataaa tgaaattttc tgagaacttg cgaagattaa gcctttttcc
     1681 aatagcagag tcaagcaact tattatatgt ggtgaaatat tcatccaaag gtagattttg
     1741 ccaaaaactg cgtttctttt ccttgcccat gtagtatata aatttttata aaagtagaat
     1801 ttgtttaata gagcagtatt atcttttgtt aagattgaga taaataattt aaaagtgtaa
     1861 aacagtattc ataaaaagtc aattcgatac agagagaaag ttataaaaaa aatctacttt
     1921 tgacatacta tataaaaaaa taaaacaatc tataaaacta aagaaataaa ccaaatgctt
     1981 aaatatattt tcatagcatt attgaaaaac tatacagaca atgaaagtct gatcaataaa
     2041 ttatgggatg aaatagagca acattatact gataagaaaa gacactatca tactttagaa
     2101 catttaaata agctctttga tcatttaagt gaagtaaaaa atgaaattca gcactggcat
     2161 atactcttgt tttcattatt ttatcacgac attatttata acacattaaa gtctgacaac
     2221 gaagaaaaga gtgctgcact tgcagtaaag agactaactc aacttggtat agctgattcg
     2281 gaaatccgtt tatgcagtca tcagatttta gcaacaaaat cacaccaatt atcagataac
     2341 cctgacacca attattttac ggatgccgat ctctccattt taggtcaaaa ttgggaaacg
     2401 tattcaaatt acatgcaaca agtaagaaaa gaatatgcca tttatcctga ttttatttat
     2461 aaaaaaggac gcaaaaaagt tgtccatcat tttcttgcga tggaaaatat ttataaaaca
     2521 acatattttc aagtaaaact tgagcaacaa gcaaatcaaa atttacaaaa agaattaagc
     2581 attttgtaaa ctgtcaggct tccatcaagc attttcagga taaaatattt taattatttc
     2641 gggcatttct atcacttctt tctttttata atcaaaataa actatacccg tacgcacaat
     2701 tgccatcgta gtaaaaccgt tttttataaa atgtaaaacc aaatcaaaac tgtaattcgt
     2761 gcaattagta aagccgactt cacattcaat tttatctcct aaaaatcctt ctgctttata
     2821 cacaatagaa tgattggcaa tgatacttcc gcaattattt tccaagtcca attcaggaaa
     2881 tttgataaac ttaaaaaatc gttgtcttgc tccattggca aatattaata tacgctcgtt
     2941 tcccatatgt tcggcttcat tgatatctgc gttttctata gagaaaaact ctctgtagat
     3001 aaaatgttcg ggtaaattaa tttttattct ttccatgata attttaaaat actaaacgtg
     3061 cctgaatcgg tgcatccagt gtcgaaaatt gtttgtctat atatttgtaa taggcagcaa
     3121 tggcaatcat tccggcatta tccgtacaat attgaaacgc cggaatatag acattccact
     3181 tctgtttttc tcccatttgc tgcaatgcat ttcgcaaacc cgaattagca cttacacctc
     3241 ctgcaatagc tacttctttg ataccggttt catttactgc acgcttcagt tttttgagta
     3301 aaatagtgac gatacttttt tgtaccgaag cacaaatatc attcaaatta tcactgataa
     3361 aatctggatt ttgtttttct tgcttttgta aaaaatacaa aatatttgtt ttcaaaccag
     3421 aaaacgaaaa attcaagcct tcaatctgag gttcattaaa aacgaaagca tccggatttc
     3481 ccaaccgggc atatttatca attaatggtc caccgggata ggggaaatga agcaatttcg
     3541 cagttttgtc aaatgcttct ccaactgcat catccagtgt ctgacccaaa atctgcatat
     3601 cgttataact atttaccctt accagttgcg tatgacctcc acttacggta aggcataaaa
     3661 atggaaattt gggtttgggc tcatcaataa aatgagctaa aatatgtgca tgcatgtggt
     3721 gaatagcaat aatcggaatt tgcagacatt gtgccattga cttggcaaaa gtacccccta
     3781 ccagtagcga gcccaaaaga ccgggtcctt gcgtatacgc aattgccgat aaatcttgca
     3841 aatacacatt agccgtttgt aaagcctgct ggataaccgg tacaatattt tttaagtgct
     3901 cacggctggc caactccggc acaactcctc catactgttc atgtattttc tggttggcag
     3961 taagattaga aattatttta ccatccacac ataccgctgc tgatgtatca tcacatgaag
     4021 attctatggc taacaagacg atttcgggca tacattttgc ttaaaaaatg gtagttttgc
     4081 aagtttagat atagtgggca aattttttaa atatgtattc cgtacgttat tgattttatg
     4141 tgcaattctg ctcatatcat ttatcacgct ctattatctt atacaacaac ccaaatacca
     4201 aacacttgca gttcgcaaag taagtgaaat tttatcaaaa caattaggca gcgaggtaaa
     4261 agttgactcc gttaatctaa aattcatctc aaccatccag attcacaaag tttatttgtc
     4321 tgaaaaaaac aaacccaacg acactatttt atttatcaat cgcttggatg ccagcctgat
     4381 gctgggcaaa acactgatgg agcaaattag tagcttgcaa tctgcaaaat actatgtcga
     4441 tgatgtaaat atagacggcc tcaaatttca tggttaccga ggattaaacg acagtatcta
     4501 taattttcag tttttactgg acaattttaa atcaaaaaac aataaaccaa cacctaaaaa
     4561 agcaaagaaa cctattgaat taaggttaaa tgaactcacg ctgacaaatg gaaatttggt
     4621 attggatgac atttacaaag accaacgttt tgatattcgt tatactaaag tagacataaa
     4681 tgtacgtcaa ctggatatca atcacttgaa aattgatgct aaaaaggttg tcttggaaga
     4741 tccttatttt tgtatgacgg attataacaa cattccttct aagcccaaaa taaaaaaacc
     4801 tactttaggt tttaaaatac aagaattggg taataagctg aatattaatg tcgacaaact
     4861 gacacttgta aatgggcagc atcgcctcga ctttcagcat aaagatcaaa aagcgggtaa
     4921 tttcctcatt tcacaaatga atatccaaaa tattgtcctt aatatggaca actatgtttg
     4981 ggattcgaca ggcatgaccg gaaaactgac taaccttagt gccaatgccg ataatcaaat
     5041 caaattgaaa aacttatccg ggcttattca tctggataat ggcggcatct atctcgataa
     5101 tgccgattta gccttgaata attccatatt aaagggcaac ctatctttac aatttttaac
     5161 cggacactgg aactcattaa gcaatttcca ggacgaagtc atgctgaaag cagatttgaa
     5221 agaaacaaaa accaatggcc atgacgtagc actttttgca ccaaaagtac aaaaatacat
     5281 tccacccaat acccgcttag agggacttat aaggggtcgt ttatctaatt tgaaagttga
     5341 aaatctaaag ctcacggctg cagataatac ggtgattgat attagcggaa atatcaaagg
     5401 cttgcccaaa attcaacaaa cactgtttga tttaaatatc caccaactta aaacaaacgc
     5461 ttcagaacta aaacagatat tgacatttgt gaaattgcct aaacaactcg acaatgccgg
     5521 caatattagt ttcaagggct cgtattttgg attcatcaat gattttgttg caaaaggaaa
     5581 tctcacaaca accaatttgg gtaatctcac gacagatttg cgtatgcgtt ttcctaaagg
     5641 caaatctccg gaatattccg gcaatgtgac tgctaatggt ttgaatttag ctgaattgac
     5701 cggaaataaa aaactcttag gcactgtaga tttagatatt gctgccaatg gaaaaggatt
     5761 taacgccaag gaactgagta ctgatttaaa aggaaccatc cgcaattttt atctcaatgg
     5821 ttttgttttt gataaaatac tggtagatgg atttttagac aagaaaaaat ttaaaggaaa
     5881 ggcatttttc gacgataagt gttttctggt ggatttcaat ggcatggtgg atttcaataa
     5941 tgagttgcca aaatttgatt ttactaccag cataaaaaat gcagacctta acaagctaca
     6001 tcttaccaaa gatacactct tggtatcctt ggatggcgaa gtacacggaa gcggcaaaag
     6061 cttagacaac ctgatagccg caggtaactt cagcaatatg atgatacaaa atcagaaaga
     6121 tattctcgta ctttctgatg tggctatgaa tgtaaaaaac agccctactt tcagagatta
     6181 taatattata tccgatcagt ttaatgcaca cgtaaacggc aactttaatc ctttgaatat
     6241 tgtaccgtca atgaaagtat ttttaagtca gtacagtaaa ctaattaagc caacagcaaa
     6301 agatttaaaa ttagcgaaac cacaacaatt gaatgctgat attaagctta aatctgattt
     6361 tggtattttc aaagtattcg tacccaaagt aaaatatctg agtgaagtag aaataaaaag
     6421 caacatcaat acaacggata agaaactaac cttaacagcc aatctcgatt cagcagttta
     6481 tgataaactg gtgatgaaca aaataatact ggatgcagaa acaagagaaa ataatttagt
     6541 tgctgatatt gtgctggata aattacagag cggtaaaaca atagtgaacg atataggggt
     6601 caatcttact tcttcagcag aaaacttatt aaccaatatc agtatttcaa atgatactgc
     6661 taaaaactca gttcgtttga cttcttcttt agatttcaga ggtgattctg tcattgcaaa
     6721 aatcttagat tcaaaattaa aattaaacaa caaaatctgg acggttgctc ctaacaatca
     6781 gctcaccatc gtagattcta tttttctcac acaaaatatc atactcttac aagatgatca
     6841 aaaaatcagt attataaatg gtcgcaactc tcttgaagat gctaaaataa acatcgaaaa
     6901 cctcaacctc acagacatcg tgcaacttgt agatacctct catattttac aaaacggatt
     6961 tttatcgggc aatgtcagtc taaaaaacat attgacaaaa ccactcgtaa atgctgattt
     7021 aaatatcaat gaattgcaag tagctggata taaagtaaaa atagtcggac tcagcggcat
     7081 ttatggccgt gatggtaaga gtatagtgga agtcaacgga acactggatg acaaagatta
     7141 tcagcttgcc tttgacggta cctatgatat gcaaatcaaa ggcaaagaaa aatttgatgt
     7201 tacagctgac atcgaaaagt taaacattag ctttttagaa accattctga aaaaagaatt
     7261 attagtacct cgtgcttttg taaaaggaca agtaaacgta tcagggaatt taaaaaatcc
     7321 tatactgaat ggtactgctg aaatcataga taccgcagaa ctcaaattca gaatgcttgg
     7381 tactacattt aaactctata aagaaaaaat aaaactgacc agagaaggat ttgattttgg
     7441 tgaaatgaca gtttatgaca attttggcaa tactgcttta ttaagtggca aactgactca
     7501 taccggattt aaaaattttg gggtagaaaa tgtagtattc agtgccccta tacagtacaa
     7561 ttttatgaat acaacctatg aagacaatca ggatttctat ggtaaagttt ttgcaaaagg
     7621 agatgttttc atgaatggga gttttaatga tttaagtata aaaattccag atttaactac
     7681 tttaaaaaat tcagaattca acctgccggt ttccgataaa gccgcagaca aagggtatgc
     7741 ctggataaaa ttcatcgacc cgaaagacac atctaagagg gtagcctata aaaccaagct
     7801 tagcggtatc aatatcgatt tgaatgtgaa tgcgacaaaa gatgccgtgg ttaatctcat
     7861 acttgaccct tcatcaaatg ataaaattac cggccgcggc tatggcaata ttaatgtcgt
     7921 gatggataaa gcaggtaatt ttaatattta caatacttat tatttgacag aaggcaagta
     7981 cgactttact tttcagggaa ttttaaataa aacattcgtt ataaaagacg gcagtaaaat
     8041 tattttcaat ggaaagcctg atgatgcgga gttagacata aaagcactgt ataatgtaaa
     8101 aagtgcttca gtccgcaact tgtttgatac aattacacaa gacgaacgca atatcagaac
     8161 gaaatccttt cctattgatt tgaacctgaa tattacagga tcactaaaga aaacaaaagt
     8221 aagtttcgat atctcggcaa caccgggtac agcatcagcc aattctgatt tattgggacg
     8281 aaaactggcg gaaatatcca ataatgaaaa tgatgtgaat aataatgcgg ctaccttgtt
     8341 gctgttcaat tccttcttcc ctaccggcac ctcatcagac caaaggtttt cgggtgcttc
     8401 tagtagtgta accgatttat tactcgggca aatttccaat ttgatttcgc aaggattagg
     8461 taagctgata aaaggtgcat ctcttgattt agttttgaac gaactcgaaa cgcaagaaag
     8521 aaatttcgga tttacttaca aacaagagct aataggtggc agattaattt taaccatcgg
     8581 cggtaatgtt aactttggca atagttctac cattaatgca accagtttat ctcaacaaaa
     8641 ttctaataat gcaattgccg gcgattttat gattgagtat ttagttacca cagatggcag
     8701 aatcaggcta aaagcatacg gcaaagcatc gaattacgat atatttggtc aagaccggtt
     8761 cagaacaggc ggtgccattt ccttccagaa agattttgat gactttaggg atttatttaa
     8821 aattcaaaat aaacgaaata aaccgatacc tgtgttagac tctctaccac ctgcaccacc
     8881 accggctgat acgggcaaac taaaagaaac agcgaatata aaataattcg ttaaaactca
     8941 tttttttgct atttaaagat aaattcagat tttcgtcttt caactcgaat aaatgaaact
     9001 atattccact aacaaaatat ctcctgatgt agatttgaaa acagctgttt tgcaaggact
     9061 tccacctgac aatggcttgt atatgcctta cgaaattccg attttgcctg aaggattttg
     9121 gaaaaatctc agcagctatt ccttttctga aatggctttc catattgcta agaatttgtt
     9181 tggtaatgat atctcagaca aagatttaaa tcaaatcata catcaggcat ttacatttga
     9241 agccccgcta attgaagtag gcgacagtta tattcttgaa ttatttcatg gccctacgct
     9301 tgcattcaaa gattttggtg ctcgtttcat gggacaacta tccggatatt ttttgagagg
     9361 caatgacaaa caagtggata ttttggtggc cacctccgga gataccggct cagcagtagc
     9421 acatggattt tataaagttc cgggcgttca tattaccatt ttatatccta aaggcaaagt
     9481 gagtgaaatt caggaaaaac aatttactac gcttggcgaa aatattactg caatagaaat
     9541 tgatggcact tttgacgatt gtcaggcatt agtaaaacaa gcatttctcg ataaggaatt
     9601 aaatgaaaaa cttacgttgt cgtctgctaa ttctataaat attgcccggt taataccaca
     9661 atcattttat tatatagacg cttacagaca actggatgac aaaagtttgc ccttatattt
     9721 ttcagtaccc agtggcaatt ttggcaactt gactgctggt ttatttgcaa agaaaatggg
     9781 attgccggtt acgaaattta tagcttcaaa caatgccaat gctgtattta ctgactatgt
     9841 acattccgga aattttaaac caaagccttc tgtacatacc ctttcaaatg caatggatgt
     9901 tggcaatcca agcaactttg cacgcatgat ggatatttat caacaaaggt tggatgattt
     9961 aaaaaacgat attacagctt atagcttcaa tgatgaacaa actcaaaaat gcatcaccga
    10021 ttatgcattg aattatgatt atatcctatg cccacataca gctgtgggtg tattgggtat
    10081 gaatcaattt attgatgatg aagatctcaa tccgctttat atcaacgcaa ttacactatc
    10141 cacagcccat ccgattaaat tcggcgaaat cgttgaacca ttaattcaag ataaaattga
    10201 tattccggaa cgattaaaag aaattataaa aggagaaaaa gtttcacttt cacaaaaagc
    10261 cacttttgaa gaattcaaat cttacttact caatcgttaa tactaacctg agttcgggtt
    10321 aagaaaatta aggtgaaaaa aatagttgat ttctcatt
//
LOCUS       DAOQII010000066        10268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3396_length_10268_cov_4.413003, whole genome shotgun sequence.
ACCESSION   DAOQII010000066 DAOQII010000000
VERSION     DAOQII010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3396_length_10268_cov_4.413003"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..1551
                     /locus_tag="PLF48_04710"
     CDS             295..1551
                     /locus_tag="PLF48_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48658.1"
                     /translation="MLRYFILLFIVILNNCKSVLAQCGARYDSVLFPVTKYADIQYGA
                     NYDNKNQLTHLLLDVYTPDPNADTERKRPLIIFTHGGSFVGGDRQDQHIDSTAHYFAS
                     LGYVTANIEYRVEQTVFISPFVNFADPNNFYKAILRGAHDVKAAIRFFKKDVIQQANT
                     YGIDTSKIFLYGSSAGAIISLHAVFLDDTLEMNNTYKTNIAALGGLEGNSGNSGYSSK
                     GVKAIVGCSGAVGSTGWLTNNTDIAYLGFHNSIDLTVPYDIGCFITVACFIGQFYGSK
                     QIYQKAHSLGMNCKLVTVNGYGHPVDEYDKTASYLMIRNETRKFLKNMVCPTVSGIMQ
                     REIVSLKIFPNPSIGIFYLDLPAELRNKSVNISIYNALGQQVWYEQNNTNAVLELHTT
                     LPKGNYFIRIDKGTNDKIYTATVVID"
     gene            complement(1552..1806)
                     /locus_tag="PLF48_04715"
     CDS             complement(1552..1806)
                     /locus_tag="PLF48_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNF48659.1"
                     /translation="MGILSWIVVGLLAGILGKVLNPGNDNEGWIMTIIIGIAGAFIGG
                     FISKLIGFGDVNGINIKSILIAGVGAFILLFAKKMLSARK"
     gene            complement(1909..3033)
                     /locus_tag="PLF48_04720"
     CDS             complement(1909..3033)
                     /locus_tag="PLF48_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNF48660.1"
                     /translation="MEEQQSKTKKIIMPIIGVLLLVAAIIFAVKKISYAMHNEDTDNS
                     QLETNIVPMAARISGYVDAIFVVENQRVHKGDTLVKLDDRDLKIKLKQAEINLKNAQA
                     NVHVVKSNVQTADASAGVSATSITTALANIESAKANIESAKVRAWNANENFKRYQQLF
                     NETSATQAQYDQALTEKQSAEKQIQIAEKQLGIAQAQLKVAQQSASAAKTQASGVGTQ
                     IEVANVNIEQRQADVDFAKLQLSYAYITAPSDGYISKKGIQVGQLVNPGQNLMSVVDD
                     SQLWVTANFKETQIEDMKIGQTVEIDVDAYPNHVFTGKIESIQAATGSKFSLLPADNA
                     TGNFVKVVQRIPVRIAINDDKGDNFFLRAGMNVTVKVKVK"
     gene            complement(3074..3715)
                     /locus_tag="PLF48_04725"
     CDS             complement(3074..3715)
                     /locus_tag="PLF48_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF48661.1"
                     /translation="MKFKVLDDISFNVYKGEFLTIIGKSGCGKSTLLYILSTMDTEFD
                     GNLIIDGQDISRWKQDKLAKIRNEKIGFVFQFHYLLGEFSCLRNVMIPALRLGKFSEE
                     EIEANAYQKLELLGLKEQALKPANKLSGGQQQRVAIARALINEPLIIMGDEPTGNLDS
                     QNTDNVFSIFRELTQEFGQTIIAVTHDTDFAKSSDRTIEMQDGKIINQDYKFD"
     gene            complement(3889..5076)
                     /locus_tag="PLF48_04730"
     CDS             complement(3889..5076)
                     /locus_tag="PLF48_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF48662.1"
                     /translation="MSNKKMTLVAAWGVMLGIAVFIFMNTLMAGFDKQSAESFFKSIP
                     HIRIYKDDVISKPLSNNTNKTPVIINPKIVPANNTIDNPKAVIDLLNHQPEVSFVTPQ
                     INAGIFFNNAKTQISGKAIGIYPDEANNMFNMQSFIVEGSIDNLKSNINGMLLGSGVA
                     EKMNVSVGDNISVTSSAGITKVCKIVGIFKTNNSIVDKTTSYINLAFAQQLLKKNSTY
                     VTDINVNLTDYKTAKVYAKNLEQLTGYKSEDWETANETYMAATRMRAIIIRFVSISIL
                     LVAGFGIYNILNMTIMQKINDIAILKAMGFSGRDVIRIFVGQAMIIGLLGILIGCVAA
                     TIMISQMQKVYVGGDIGYFPIYFNLQKYFLGIAFGFLVTFFAGFIPARKAANIDPVAI
                     FRK"
     gene            complement(5126..6271)
                     /locus_tag="PLF48_04735"
     CDS             complement(5126..6271)
                     /locus_tag="PLF48_04735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF48663.1"
                     /translation="MKSLEKKIIRIKQGQNKIMAILLFYIFILNACNADKSKEVKPIR
                     KDIDEIVFASGTLESDNKYNLMAQMDGTLTQFSIKEGNLVSSGQIVAYIDNKQNNINT
                     QTAQEQLDIAKYNLSNNAPLLQQIQANIDAANDKLAQDELQLKRYENLYNENSVTKAE
                     LEKYQLAVKTSKAGLSALKQQYKSVQQQSKQQYIQQSNAVKVSQTIQAYNQVKIVAPG
                     KVYKKTKQVGDYVRRGDIIAVIANEGLLYAKLNVDENSIDKLRVGQQVTIQLNTQKDK
                     KYQAAISEIMPQFDEQSQSFIVKVQFADKPDFTINGTQLEANVLIGEKKNALLIPRNY
                     MTFGNKVQVKGNDKLVTITPGIVSTEYVEVLSGISENDILVPVKPQR"
     gene            complement(6278..7591)
                     /locus_tag="PLF48_04740"
     CDS             complement(6278..7591)
                     /locus_tag="PLF48_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF48664.1"
                     /translation="MKKHAISLFFLFVTVLAFGQETKTISLQEAFQLAAQNSKQLKLD
                     SLKKESLSIKKQQTQNAMFPVLGLTSAYTRLSNNIEPFSISVPGLGSMALNPQILNQF
                     VNRFSVQQPIFQGLRNWNNLKAIKLQNTAADFDILKDNQDLKWNIVQTYYNLYKLQQT
                     SNLLDSTIAQTQVRINDLIKFKKEGLILNNDVMRAELQKTNLLVSLADVESNIETLNY
                     NLNILLGLGGNTKLIVSDIPGMATYIAISGDYNQAFAGRPELNALDYKNKAAAYQVKA
                     AKSAYMPTINFVGNGYYNNPNQRVFPQENKFKATWDIGVSVSWNIMQAYTAKAVVNES
                     KNQKLQLENAIAQAKEGIQMEVNANLKAYQVTQMKIDLAQKAIEQAKENKRILNNRFN
                     AQLALLNDVLEADILLLQAQTTLLNAQADQAIAYYKLQKSLGEIK"
     gene            complement(7609..8226)
                     /locus_tag="PLF48_04745"
     CDS             complement(7609..8226)
                     /locus_tag="PLF48_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNF48665.1"
                     /translation="MSIKTTDKRDEILDAAEILFAEHGFEAVSVREISKAADINIAMI
                     SYYFGSKEKLYEEVVNRKLISLNQLQMHLNKLPTYHQQLITCLDILLDKLIERRNFQN
                     IIFREITVNQRTKMSDKIASALHQNFQFIADIIQKGIKNNEFKKVDVNLTVMSVFGIL
                     RMYVTSGNMACRIMNLHQSEDIFSTTNKKRLKTHLHEMLTNHLIN"
     gene            complement(8336..9100)
                     /gene="mtgA"
                     /locus_tag="PLF48_04750"
     CDS             complement(8336..9100)
                     /gene="mtgA"
                     /locus_tag="PLF48_04750"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNF48666.1"
                     /translation="MKAKNDFGTLGEIKMKKSLNFIWKWLKRLTIAFFILSIVSVILF
                     RFIPVPCTPLMVIRAVEQIAHGEMPKIDKDWRRLSKISADMPLAAMAGEDQKFQNHFG
                     FDVDAMKKAMKRNERLKAKGKPIKGGSTISQQCAKNVFLIPVRSYIRKAFEAYFTVLI
                     EVFWSKDRIMEVYLNVIEMGDGIYGIQAAAKNYYKKDAKDLTAAECAQIAAVLPNPRK
                     WNAGKPNAYVLKRKNWILRQMRNLPQDEEDPEEAEK"
     gene            9141..10256
                     /locus_tag="PLF48_04755"
     CDS             9141..10256
                     /locus_tag="PLF48_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNF48667.1"
                     /translation="MQDLPYPNSWNILFGNLLQLRDERIDFLMKNRNELGDIYQIKVP
                     TRKVMVLSDPDWVKYVLVDNNKNYIKSFAYDSIKMFLGNGLLTSEGEFWKRQRRLAQP
                     AFHKEKLALMFKNMLEQTRQVVDEMKPYAASGQLFNLNKVLYKLTLNVVNNTLFYNEV
                     DNTTDRIYHLVSKASEIISQRIDSLVQLPEWIPTQRNLRQKQIIAEMDALFYDVIERR
                     RKTKVDFEDLLSMLMDAVDKETGETMSNKQLRDEILTIFVAGHETTQIALAWTFYLLG
                     QNPEKKAILQREIDEQLKGELPTPENIKGLHYLKKVIDESMRCFPPAWIMGRRNLATD
                     HINGYEIEPGTNIIMPIYVIHHREDIWEKPFFDTDPF"
BASE COUNT         2966 a         1861 c         1829 g         3612 t
ORIGIN      
        1 gctatacttt ttgttccaca ggttgcaaaa aaaatgaatt taattctctt gtagtgaact
       61 cctcaatagc tttgaaataa ttataaactt gttttgctta agcgttaaac tgcgcttttg
      121 tgtaaaatct tattgccgtg gatataatgg cgtttaaaat cttgttcatc gagaattgtt
      181 ttaataatct taccctaata tattatacgc ttttttttgc attaaaagat actcttattt
      241 cctaaagtta tcgacattga atttactttt ctgatttatt tgtaacttca tcttatgcta
      301 agatatttta ttttgctttt tatcgtaatc ttgaataatt gcaaatctgt tcttgctcaa
      361 tgtggtgccc gatatgattc ggttttgttt cctgttacaa aatatgcgga tatacaatac
      421 ggtgctaatt acgacaataa aaaccagttg actcatcttt tgctggacgt gtacactccg
      481 gatccgaatg ctgatacgga aagaaaacga cctttgatta tctttacgca tggcggctcc
      541 tttgttggcg gtgacagaca agatcagcat atagactcaa cggctcatta ttttgcttct
      601 ttgggttatg taacggcaaa tatcgaatac cgtgtagagc aaacagtttt tatttctcct
      661 tttgtcaact ttgccgaccc aaacaatttt tataaggcta ttttgcgagg cgcacatgat
      721 gtgaaagcgg caattcgttt ttttaaaaag gacgttatac aacaagccaa tacttatggt
      781 atagacacct ctaagatttt cttatacggt tcttcggcag gtgcaattat ttctttgcat
      841 gccgttttct tagatgatac tttagaaatg aataacacgt ataaaacaaa tattgctgct
      901 ttaggtggct tagaaggaaa tagcggcaat tccgggtatt cttcaaaggg ggtaaaggcg
      961 atagtgggtt gcagcggggc tgtgggcagt acaggctggc tgacgaacaa tacagatatt
     1021 gcctatcttg gattccacaa cagtattgat ttgactgtac cttatgatat aggctgtttt
     1081 atcacggttg cctgttttat tggacagttt tacggaagta aacaaattta tcaaaaggca
     1141 cacagcttgg gtatgaattg caagctggtg accgtaaacg gctatgggca tccggtagat
     1201 gaatatgata aaacggcaag ctacctgatg attagaaatg aaaccaggaa atttctcaag
     1261 aatatggttt gtccaacagt gagtggtatc atgcaaagag aaatagtctc tctcaaaata
     1321 tttcccaatc cgagtattgg aattttttat ttggatttgc ctgcagagtt gcgaaataaa
     1381 agtgtaaata tctccattta taatgcactt gggcaacaag tctggtatga acaaaacaat
     1441 actaatgccg ttttggaatt gcatacaaca ctgcctaaag gcaattattt tatcaggata
     1501 gataagggaa ctaacgataa aatctacact gcaactgtcg tcatagattg attattttct
     1561 ggcactcaac attttttttg caaacagcaa aataaatgct ccaactccgg caattaagat
     1621 acttttaata ttaatgccgt ttacatcgcc aaaaccaatc agtttggaga taaagccacc
     1681 gataaatgcg cctgcaatac caataatgat ggtcataatc catccctcat tgtcatttcc
     1741 ggggtttagc actttgccca aaattccggc taacaatcct acaacaatcc aagataaaat
     1801 tcccataagt ttattttttt agcaaattaa tacaaatctg ctaaattgtc aatcaggtgg
     1861 ttctcgggtg tcacgattat cgaggcacat gtagttgctt atgtgcaatt atttcacttt
     1921 cactttaact gttacattca tcccggcacg taaaaagaag ttatcacctt tatcatcgtt
     1981 gatggctatg cgaacaggta tgcgttgtac tactttcaca aagtttccgg tagcattatc
     2041 agcaggcaat aatgaaaatt ttgagccggt agcagcttgt atggattcta tttttcctgt
     2101 gaaaacatga ttcggatatg catcaacatc aatttcaacg gtctgcccga ttttcatatc
     2161 ttcaatttga gtttccttaa aattagcggt tacccaaagc tggctatcgt ctacaacact
     2221 catcaggttt tgtccgggat taaccagttg tcccacttgt attccttttt tagaaatata
     2281 tccatcactc ggagcagtga tataggcgta tgacaattgc aattttgcaa aatctacatc
     2341 ggcctgacgt tgctcaatat tcacattggc cacttcaatt tgtgttccca ctccggatgc
     2401 ttgtgtttta gccgctgatg ccgactgttg cgctactttc aattgtgctt gcgcaattcc
     2461 caattgtttt tctgctattt ggatttgttt ttctgcactt tgtttttctg ttaaagcctg
     2521 gtcgtattgc gcctgagttg cagaggtctc attgaaaagt tgctgatatc gtttaaagtt
     2581 ttcattagca ttccaagctc gcactttagc cgattcgata tttgctttag ccgattcgat
     2641 attggctaat gccgttgtaa tggatgtggc tgatactcct gctgaagcat cggcggtttg
     2701 cacattgctt tttacgacat gtacattggc ttgtgcattt tttaagttga tttctgcttg
     2761 tttgagcttg atttttaaat ctcggtcatc taattttacc aaggtatctc ctttgtgcac
     2821 acgttgattt tcaacaacaa aaatagcatc aacatatccg gagatacggg ctgccatagg
     2881 cactatattc gtttccagtt gtgagttgtc ggtgtcctca ttatgcatgg cataactgat
     2941 ttttttaaca gcaaaaatta ttgcagccac taataacaat acaccaatga ttggcatgat
     3001 gatttttttt gttttagatt gttgttcttc catgttgaaa tttataatgc ttatttaaaa
     3061 atcttttttt cagttaatca aatttataat cctgattaat gattttgccg tcttgcattt
     3121 ctattgttcg gtcagatgat tttgcaaaat ccgtatcgtg cgtaacagca attatagttt
     3181 gaccaaattc ttgagtcaat tccctgaaaa tggaaaagac attgtcggta ttctgactgt
     3241 caaggtttcc tgtaggttca tcacccataa ttattaacgg ctcattgatt aatgcccggg
     3301 caatagccac gcgctgttgc tgtccgcccg aaagcttgtt ggctggtttt aatgcttgtt
     3361 ctttcaatcc caataattca agtttctgat aagcatttgc ttcaatttct tcttccgaaa
     3421 acttacctaa ccgcaaagca ggaatcatga catttcgaag acaagagaac tcacctaaaa
     3481 gatagtggaa ctgaaagaca aaaccgattt tttcattcct gatttttgcc agtttatctt
     3541 gtttccatct cgatatatct tgaccgtcta taatcaaatt gccatcaaat tcggtatcca
     3601 tcgtagataa aatatatagt aatgtagatt ttccacatcc ggattttcct atgatggtaa
     3661 gaaattcgcc cttataaaca ttaaacgaaa tatcatccaa taccttgaac ttcaccggtt
     3721 catagaagta tttgcttata gcatgagttt gtaatataat ggacttgttt ggcattagaa
     3781 tttggtcatt ggttattagt cattggttat tagtcattgg ttattggtta ttagttattg
     3841 gttattagtt atttataatt tataattcaa cattcaacat tcaatatgtc attttctaaa
     3901 gatagctaca gggtctatat tggctgcttt gcgggcgggt ataaatccgg caaaaaatgt
     3961 aaccaaaaac ccaaaagcaa ttcccaaaaa atatttttgc aaattgaaat aaatagggaa
     4021 gtagccgata tctccaccta catatacttt ttgcatttgt gatatcatga ttgttgcggc
     4081 tacgcagcct attaaaattc caagtaaacc tataatcata gcctgcccta caaatatacg
     4141 tatcacgtca cgtccactga atcccattgc tttcaggata gcaatatcat ttatcttttg
     4201 catgatagtc atgttgagaa tattgtaaat gccaaatccg gcaaccagca agatagaaat
     4261 tgatacaaaa cgaatgataa ttgccctcat acgtgtggct gccatgtaag tttcatttgc
     4321 agtttcccag tcttctgatt tatatccggt gagttgttcc agattttttg catatacttt
     4381 tgcggtttta tagtctgtga ggttgacatt gatatctgtt acataagttg aattcttctt
     4441 taggagttgc tgtgcgaacg ctaagttgat ataagacgta gttttatcca ctattgaatt
     4501 attcgtttta aagattccga caattttgca aactttggta atgccagccg atgaagtaac
     4561 tgatatatta tcgcccacac ttacattcat tttttctgct actccactgc ccagcaacat
     4621 gccattgata ttgcttttta aattatcgat actgccttct acaataaaag attgcatgtt
     4681 gaacatgtta tttgcttcat ccggataaat gcctatggct tttccgctga tttgtgtttt
     4741 ggcattattg aaaaatatgc cagcattaat ttgtggagta acaaaggata cttccggctg
     4801 atgattcaat aaatcgatga ctgcttttgg gttgtctatc gtattattag cgggtacaat
     4861 ctttggatta ataatgaccg gagttttatt ggtattgtta cttagcggtt ttgaaatgac
     4921 atcatcttta taaatccgga tatgtggaat tgatttaaaa aaagactcgg ctgattgttt
     4981 gtcaaaaccc gccattaggg tattcataaa aataaataca gctataccca gcataacacc
     5041 ccacgccgca acaagtgtca tttttttgtt gctcataatg tacgtgtacg atatttctcc
     5101 gttaacagga tgcttgtaag tcattttaac gttgtggttt taccggtact aaaatatcat
     5161 tttcgctgat gccactgagt acttccacat attcagtaga aactatgcca ggtgtaatgg
     5221 tcacgagttt gtcattgcct tttacttgca ctttattgcc aaatgtcatg taatttctgg
     5281 gaattaaaag cgcattcttt ttttcaccta tcaatacatt ggcttccagt tgtgtaccgt
     5341 ttatggtgaa atccggcttg tctgcaaatt gcacttttac aataaatgac tgggattgtt
     5401 catcaaattg cggcataatc tcacttattg ccgcctgata ctttttatct ttttgcgtat
     5461 tgagctgaat ggttacttgt tgtcctactc gcagtttatc tatagagttt tcatctacat
     5521 tgagcttggc atacagcaga ccttcgttgg caattacagc aataatatca ccgcgtctta
     5581 cataatcacc tacttgtttg gtttttttat acaccttgcc tggtgctaca attttcacct
     5641 gattataggc ttgaatggtc tgagaaactt tcacggcatt agactgttga atgtattgtt
     5701 gcttgctttg ttgttgtact gatttatatt gttgttttaa tgcactcaat ccggctttag
     5761 atgtttttac ggcaagctga tatttttcca attcagcttt ggttacactg ttttcgttat
     5821 ataaattttc atagcgtttc agttgcaatt catcttgagc cagtttatcg tttgcggcat
     5881 ctatattggc ttgtatttgt tggagcaaag gtgcgttatt acttaaatta tattttgcta
     5941 tgtctaactg ctcttgtgca gtttgcgtat tgatgttgtt ttgtttgttg tcaatataag
     6001 caacaatttg accggatgat actaagtttc cttccttgat gctgaattga gttaaagtcc
     6061 cgtccatttg tgccatcaga ttatatttat tatcagactc taaagtaccg gaagcaaata
     6121 cgatttcgtc aatatctttt cttattggtt ttacttcttt acttttatca gcattgcatg
     6181 catttaagat gaaaatataa aaaagcagaa ttgccattat cttattttgc ccttgcttta
     6241 tgcgaattat ttttttttcc aaacttttca tggcatttta ttttatttca cctaaacttt
     6301 tttgtaattt ataataggca attgcctggt cagcctgagc atttaataag gttgtttgcg
     6361 cctgcaataa aagaatgtca gcttccaaca catcatttaa caaagccaac tgggcattga
     6421 aacgattatt taggatgcgt ttattttcct tggcttgttc aatagctttt tgcgctaaat
     6481 ctattttcat ttgtgtcacc tgataagctt tcaaattggc atttacttcc atttgtatgc
     6541 cttctttagc ttgtgcaatg gcattttcca attgtagctt ctgattcttg ctttcattta
     6601 caacagcttt tgccgtatag gcctgcatga tattccagct tacactaaca ccaatatccc
     6661 aggttgcctt aaatttgttt tcttgtggaa atacacgttg attagggtta ttgtaataac
     6721 cgtttccaac aaaatttata gtgggcatgt atgcactttt agctgctttt acctgatatg
     6781 cagccgcttt gtttttataa tctaatgcat ttaattccgg gcgacctgca aatgcctgat
     6841 tgtaatcgcc ggaaattgct atataagtgg ccattcccgg gatatcgcta actattaatt
     6901 tcgtgttgcc acccagacct aacaagatat ttaaattgta gttcagtgtt tcaatattgc
     6961 tttccacatc agcaagacta actaacagat tcgtcttttg taattctgca cgcatcacat
     7021 cattatttaa aatcaatcct tcttttttaa attttatcaa atcgtttata cgcacctgtg
     7081 tttgtgcaat ggtagaatcc aagagatttg atgtttgctg cagcttatat aaattgtaat
     7141 aggtttgtac aatattccat tttaagtctt gattgtcttt caggatatca aaatctgctg
     7201 cagtgttctg taatttaata gcctttaaat tattccagtt cctcaatccc tgaaaaatgg
     7261 gttgctgaac agaaaagcgg ttgacaaatt gatttaatat ctgaggattt aaagccatac
     7321 tgcccagacc gggaacactg atgctaaagg gttcaatatt attactcaat cgagtgtaag
     7381 cagaagttaa acccaatacc ggaaacatag cattttgtgt ctgttgcttt ttaatagata
     7441 gcgattcttt tttcagactg tcaagtttaa gttgtttgct gttttgtgct gctaactgaa
     7501 aagcttcctg taagctgatt gtttttgtct cttgtccgaa tgcaagtaca gttacaaaca
     7561 ggaagaataa actaatggcg tgttttttca ttatgatgga atcaattttt aattaatcaa
     7621 atggttggtg agcatttcgt gaagatgtgt ttttaaacgt tttttgttag tagtactgaa
     7681 tatatcttca gattgatgga gattcatgat tctgcaagcc atattaccgg atgtaacata
     7741 catacgtaaa attccaaaaa cagacattac tgtaagattt acatctactt ttttaaattc
     7801 attattcttt attcctttct gtattatatc tgctataaat tgaaagttct gatgtaaagc
     7861 gcttgcaatt ttatcagaca ttttagttcg ttggttgact gtaatctccc ggaaaatgat
     7921 attctgaaag tttcttcttt ctataagttt atcgagcaaa atatctaaac aagtaattaa
     7981 ttgctgatga taagtcggca atttgttcag gtgcatttgc aactgattta aggaaatcaa
     8041 tttacgattt acaacttctt catacaattt ctctttagac ccaaaataat atgaaatcat
     8101 agcaatatta atatccgcag ctttactgat ttcacggaca ctaacggctt caaatccatg
     8161 ttctgcaaat aagatttccg cagcatctaa tatttcatcc cgcttatctg ttgtctttat
     8221 ggacatagtt aaacaattgt ttaatgccac aaatttaatt aaacgtttgt ttaattgtta
     8281 aattaaattt tcgttaaagc actttttgtt caataaaaat aggagatact aaaatttatt
     8341 tttctgcttc ctctgggtct tcttcatctt gtggcagatt tcgcatttgg cggagtatcc
     8401 agtttttgcg ttttagaaca tatgcattcg gtttccctgc attccatttt cgtggattag
     8461 gcaacacggc tgcaatctgt gcacattcgg cagcagttaa atctttagca tcctttttat
     8521 aatagttttt agcagcagcc tgaatgccat aaatgccgtc acccatttca atcacattca
     8581 gatacacttc cataatgcgg tctttactcc aaaaaacttc tatcaaaact gtaaaatagg
     8641 cttcaaatgc tttgcggata taacttctca ccggaattaa aaaaacattt tttgcacatt
     8701 gctggctgat agtacttcca ccttttatgg gtttaccctt agcctttagc ctttcatttc
     8761 tcttcatagc ttttttcatg gcatccacat caaaaccaaa atgattttga aatttctggt
     8821 cttctccagc cattgctgca agtggcatat ctgctgaaat tttactgagc cgcctccagt
     8881 ctttatcaat tttaggcatt tcgccatgtg caatttgttc aacagctctg ataaccataa
     8941 gcggtgtaca gggcaccgga ataaaacgaa ataaaatgac agaaactatg ctaaggataa
     9001 aaaaggcaat cgtgagccgt tttaaccact tccaaataaa gtttagtgat tttttcatct
     9061 taatttcgcc taaagttccg aaatcatttt tagctttcaa aatcaaatta agtagtatga
     9121 aaaatgttgg tcaataaaca atgcaagatt tgccttatcc caactcttgg aatatcttat
     9181 ttggaaatct attgcaatta cgggatgaaa gaatagattt tttgatgaaa aatcgtaacg
     9241 aactgggtga tatctatcaa atcaaagtac ccacaagaaa agtgatggta ttgtctgatc
     9301 cggattgggt gaagtacgta ctggttgata ataataaaaa ttacattaaa agtttcgctt
     9361 atgacagtat caaaatgttt ttgggaaatg ggctactgac aagtgaagga gaattttgga
     9421 aaagacaacg ccgattggct cagccggctt ttcataaaga aaaactggca ttgatgttta
     9481 aaaatatgtt ggagcaaact cgccaggttg tagatgaaat gaaaccctac gcagcaagcg
     9541 ggcaattgtt taatttgaat aaagtactct ataaacttac tcttaatgtg gtaaataata
     9601 ctttgtttta taatgaagtg gacaatacca ccgaccggat ctatcattta gtatctaaag
     9661 ccagtgaaat tatttcacaa cgcatagata gcttagtgca gttgccggag tggataccta
     9721 cgcagcgcaa tttacgtcaa aagcagatta ttgcagaaat ggatgcgcta ttttatgatg
     9781 tgattgaaag gcggcgtaaa acaaaagtcg attttgaaga tttattgtca atgctgatgg
     9841 atgctgtaga taaagaaaca ggggaaacga tgagcaacaa acagctacgg gatgagattc
     9901 ttactatttt tgtagccgga catgaaacaa cacaaattgc attagcctgg acattttatc
     9961 tcttaggtca aaatcctgaa aaaaaagcca tcttacaacg agaaatagat gagcaattga
    10021 aaggtgaatt gccaactccg gaaaatatta aggggctgca ttatttaaaa aaggtcattg
    10081 atgaatctat gcgatgtttt cctccggctt ggattatggg cagaagaaat ttagcaactg
    10141 accacattaa cggatatgaa attgaaccgg gtacaaatat tatcatgccc atttatgtca
    10201 ttcatcatcg cgaggatatt tgggaaaagc cattttttga tactgacccc ttttaatttg
    10261 gctgtcga
//
LOCUS       DAOQII010000067        10163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3451_length_10163_cov_4.449545, whole genome shotgun sequence.
ACCESSION   DAOQII010000067 DAOQII010000000
VERSION     DAOQII010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3451_length_10163_cov_4.449545"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..2624
                     /locus_tag="PLF48_04760"
     CDS             177..2624
                     /locus_tag="PLF48_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48668.1"
                     /translation="MLKKLSLLCLFFLVVIATKAQISITSLPGSYSQDFNSLSDTGIA
                     NAFNLTGWYASVPTYRANYGMMNNGALYSFGDTMPATHTDRALGTVGSSSVKPAYGVR
                     FNNNSSLSMTGMTVAYTAEQWRLGQKPAKRLDSTSFYYNIGVDSLSASGWIKVPDLGI
                     FTIDTSATAAGPLDGNLALNRKAISFTISGFVVSPGSNIWIKWVENDVSGSDDGLAVD
                     DLSVTITGSAVAACTEPTASVTNVVFSGTGLTTASGSFTGTSPASDGYLVVVDSVSTP
                     PAITDATTYTVGQTLGSGKVVSNGTSTTFNATGLVANTIYHAYVFPYNSANCSGGPNY
                     RTTSAGTDTAKTFVDACPEPTAKPTNLTFTTVNNNTISGQFTAASPAPTGGYVVVIST
                     SSNVAYPLDSTAYNVGDSIKYTSFKSKVVYVGTGTTFSATGLVPGTRYYFAVVPFNTC
                     TYGPNYLRTSPLRADTTTTGTAPLQDCVQPTGISNTSIIKVDSTTNSITIKWTNSVNA
                     DSVMVAAAPTTTIGFVTIHDSAFYAVGSTLPSNGTTPPIVYYRGTDSTLTLTGLLTNT
                     VYKILIVPFNNRNCTNGPNYAGLASTLIRTAAGTDCSQPSGVSTTSIVKLDSTTTTIS
                     IKWTNPANADSVMVAAAPTTTIGFVTIHDSAFYPVTSTLPSNGTTPPVVYYRGTDSTI
                     TLTGLAPNTVYKILVVSFNNRSCTNGPNYAGVANTTVKTGIATAVRVNNTAQFSMYPN
                     PVAANGNLTVKLEAGISGKNIVFEVTDLMGRKVSSTSVDHAATQLTIPVGDLSKGIYI
                     LSLISNNEISSATFRVE"
     gene            2697..3833
                     /locus_tag="PLF48_04765"
     CDS             2697..3833
                     /locus_tag="PLF48_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNF48669.1"
                     /translation="MRKIYTLISLHFLIASAIAGPGTYYNSLDTTKSCANFKTQLYNL
                     IKSNTTVIPYAQVDNYFNSTDYKPSETGTGNAIIDKYSSENPFGTDYCNFQYPADFCL
                     GRSSSTECFCYDKEHVFPKSWFGGTDMYPMYSDLMFIWPSDNYVNFRKGNLPLGYVNS
                     PFYTSHNGTKIGTSKTASNYNYSNTNVFEPIDSFKGDFARAYLYVITRYEDSIVNWTN
                     RSIAGNMLDGNKYPGFKSWLLQLCVKWHKLDPPSTQERSRNDAVQYLQGNRNPYIDHP
                     EWAEKVFGPNGLSASCVPTALLQKQNLSVEIFPNPVKNILHIRTENNDTYQLTLTDIL
                     GRTVFNQTALNTTFEINTHDLPKGLYLLQLNSEKATTTKQIIVE"
     gene            complement(3820..4872)
                     /locus_tag="PLF48_04770"
     CDS             complement(3820..4872)
                     /locus_tag="PLF48_04770"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HNF48670.1"
                     /translation="MKIKMLFCAGIFLMINSVFGQKTTYNIIVGGFYNLENFYDTINQ
                     PGVDDEDFTPNGSYHYTAEIFKDKVNHLAEVVSLIGTDQSPDGLAFMGCAEIENKSVL
                     ETLVNHPKLKTRNYQIVHYNGPDLRGVDCGFIYNPKYFKVLESQSITVDLKQVVNDWR
                     PTRDILYVKGILGNTDTVHIFVNHWPSRRGSTEATAPLREFAAAIDKRLSDSIMAINP
                     DAKIIVMGDLNDNPDDPSMTKVLACSSKREKTKDKGFYNPWVDYTKKGLGTLAFNDNW
                     GLFDQEVFSKGWLDNKQSGYFLHKAYIFKRDFMVQKTGKYKDYPKRTWDFNIYNAGYS
                     DHFPTYVVFLKAVTPQ"
     gene            5005..7584
                     /locus_tag="PLF48_04775"
     CDS             5005..7584
                     /locus_tag="PLF48_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF48671.1"
                     /translation="MNKNIYLSALLSTCFLLGFSKQEDDSLKVQAGVPDTSRTYLEVP
                     TVTLEDMETSDESGYSQGVSPVLNAGRDPFIQAASFSWSISRFKIRGYDADYFETYVN
                     GIPTEYIDNGFSAYNLWSGLNDVTRNRENSFGMRASTFAFGTIGGLYAIDMRASKQRK
                     QTSITISASNRTYDIRGGATWGSGITKKGWSFSASVFGRWAKQGYVKGTYMQSLSYFA
                     SIEKFFKHNNSLALTAFGVPTKQGKAANVVQEAYDLAGTNYYNPNWGLQNGKVRNARV
                     EYRHQPVIILTHEWKHDYGNLLTSIGYSFGERALSGIDKYKANDPRPDYYKYLPSYQD
                     DSLQEALVAELWQNSEYTRQVKWDDIYFANKNGESVTITNIDGDAAKSVTGKRAHYIL
                     GEDVQKYQRLNFNTVYNHTFKKLADLTAGASYQFQKVINFKRVKDLLGGDFYMDVDEY
                     IESDSVSNPSAAYSDLNNPNHAVYKGDKYDYNYSSNIHVASVWAQPTFKFKHIDFFVG
                     GKLEYTKQWREGYFKNGLNPTNSEGVSQKFSYIDYGVKAGLTVKINGKNYIYANGMYT
                     TRAPYWDNVFISPRTTNSTYNPGSEKIGSAELGYILHSNKLKLRATLFYTEFLNGSNT
                     IVYFNDDIYTMINYTISGINKRHYGGELALEAPLYKGLSLTFATSMGQYKYTSRQKGI
                     MTADDNPSLRTEEVIYSKNFNVANTPQMAYNLGLFYRSKKFWYIGAGVNFFDWIYTDF
                     APTHRTDNATDAVPYHSDLWYSIINQERTSPKGQWTLDMSGGYSWRLKSTFKNMKGKY
                     ANKYYLVINAGLSNITNNKKFIVSGREQMRFDYAEKDPMKFAPKYSYAYGINFYANLT
                     FRF"
     gene            7619..9082
                     /locus_tag="PLF48_04780"
     CDS             7619..9082
                     /locus_tag="PLF48_04780"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNF48672.1"
                     /translation="MNTTMIKRFLIAFVAITAITLSSCKKEKYDEPSGNTPDPNLTTI
                     TIDSLRKYFTSGLPITITNDVTISGIVTADDKDGNFYKQIVIDDGTAGIPVLIERSYL
                     YTDFPQGRKIYVKCKGLVLGAYHNYLQLGGFVDNSTGQPAVGNIPSDLADKVIVKGPT
                     GNAVTPIVVTYSQLNNSYQARLIKIQNTEFEEAAVGLPYADIINQQSISRNLNVCDTL
                     PGTDPKIEVRTSNYALFANELVPAGHGDFIGVYSVYNTSKQLTIRNLSDFDFSGGTRC
                     NPITVYSPADISTLRNIYTGSNVAVGSDLLITGVVISSIADSNNLAQNLTIQDNTGGI
                     VVRMASSSPNFNKGDQVQVKIGNADILQRFSNGSLQIGTIQNAKITKIASNVNVTPQT
                     VTIATLISNFNDYESELIKVTGVTISGGSGLYSGTLTFTDATGSIASFVRTATMVPSA
                     EFVNQSYLTSTVNIVGYANLNGSTKQLILRNASDVTP"
     gene            complement(9604..>10163)
                     /locus_tag="PLF48_04785"
     CDS             complement(9604..>10163)
                     /locus_tag="PLF48_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNF48673.1"
                     /translation="ANAIATTNTAAAAAMLMWVFVDALRGYKVSSIGACIGAVVGLVA
                     ITPACGYVNVGESIAVGALSALVSNFAVHYKNKSTLDDTLDVFPCHGVGGIMGMFFTG
                     VFAADYGTGLKTADWGLMYGVYHTFTVHIIALIGVAAFAFTGSYILYFITDKIQPMRV
                     SAEEEELGLDITQHGESYQTLSIDL"
BASE COUNT         3362 a         1942 c         1845 g         3014 t
ORIGIN      
        1 aaaggaggtg gttctatata aaaaatcaac tattttttaa ttgaaacgct tatccagaac
       61 tcaggttata aacaaacaac acaagatatt cacataaaat actaatatta aagccatttt
      121 taacaaatgg ttttaacaaa atttaataat tttgcatttc gaataaaatt catcttatgc
      181 taaaaaaatt aagcctttta tgtctgttct ttttagttgt catagcaaca aaagcacaga
      241 tttcaattac ttctttaccc ggaagttatt ctcaagattt caatagccta tccgatacgg
      301 gtattgcaaa tgcatttaac ctaacaggtt ggtatgcatc tgttcctact taccgagcaa
      361 actatggcat gatgaataat ggtgccttat atagttttgg cgatactatg cctgcaactc
      421 atacagacag agctcttggt actgtgggtt caagttctgt aaagcctgct tatggggtac
      481 gttttaacaa caactcttcc ttgtctatga ccggaatgac tgttgcttat actgcagaac
      541 aatggagatt agggcaaaaa cctgctaagc gtttagactc tacttcattt tattacaata
      601 tcggggtaga ttcattaagt gcaagcggtt ggattaaagt gccggatttg ggtatattta
      661 caattgatac ttcggctaca gcagcaggac ctttagatgg taatttagct ttaaacagaa
      721 aagcaatttc tttcactatt tcaggttttg tagtttctcc gggttccaac atttggatta
      781 aatgggtaga aaatgacgta tctggtagtg atgatggatt agcagtggat gatttaagtg
      841 ttactattac cggaagtgct gttgcagcat gtacagaacc tactgcttct gttaccaatg
      901 tcgttttttc aggaacaggt ttaactaccg caagcggttc atttaccgga accagcccgg
      961 cttcagatgg ttacttagta gtagtggata gtgtatctac acctcctgca attacagatg
     1021 caaccacgta tactgtagga caaactttag gaagtggcaa agttgtaagc aacgggacaa
     1081 gcactacatt taatgccacc gggttagttg caaacacgat ttatcatgca tatgtttttc
     1141 cttataatag tgctaactgt tcaggaggtc cgaactacag aactacatct gcaggtacag
     1201 atacggcaaa aacatttgta gatgcatgtc cggagccaac agcaaaacca actaacttga
     1261 catttaccac agtaaataat aatacgattt ccggacaatt cactgcagct tcacctgctc
     1321 caacaggagg ctatgtagtg gttatcagta cgagctcaaa tgtggcttat cctttagaca
     1381 gtactgctta caatgttggc gattccataa aatacaccag ctttaaatct aaagtagtat
     1441 atgtaggaac aggaactact ttttcagcaa ccggattagt gccgggaaca cgttattatt
     1501 ttgcagttgt tccgtttaac acttgtactt atggtcctaa ttatttgaga acttctccat
     1561 tacgagctga taccacaact accggtactg ctcctttgca agattgtgtt caaccaacag
     1621 gtatttcgaa tacatctatt ataaaagtag actctacgac caactccatc actataaaat
     1681 ggacaaattc tgtgaatgca gacagtgtca tggttgcagc agctcctact actactattg
     1741 gatttgtaac catacatgac agtgcttttt atgctgtggg ttctaccttg ccttcaaacg
     1801 gcaccacacc tccgattgtt tactacagag gtactgattc tactttgaca ttaaccggtt
     1861 tactgacgaa tacggtatat aaaattctga tagttccatt taacaacaga aactgtacta
     1921 atggaccaaa ttatgcaggc ctggcatcta cgcttatcag aacagctgcc ggaaccgatt
     1981 gttctcaacc aagtggtgtt tctacaacat ctattgtaaa actggattct actacaacta
     2041 cgattagcat taaatggaca aatcctgcaa atgcagacag tgtgatggtg gcagccgccc
     2101 caacaactac aattggcttt gttacgattc atgacagtgc attttatccg gttacttcaa
     2161 ccttaccttc aaatggaaca actccaccag tagtttatta tagaggtaca gattcaacaa
     2221 ttaccttaac aggacttgct ccaaataccg tgtacaaaat tcttgttgtc agctttaaca
     2281 acagatcttg tacaaatgga ccaaattatg ccggagtagc taatactact gttaaaaccg
     2341 gcattgctac cgcagtgaga gtaaataata cggctcagtt tagcatgtat ccaaatccgg
     2401 tagctgctaa tggcaatctt acggttaagt tggaagcagg gatttccggt aaaaatattg
     2461 tatttgaagt tactgatttg atgggcagaa aagtaagcag cacatctgtt gatcacgcag
     2521 caacacaatt aacaattccg gttggagact tatccaaagg catttacata ttaagtctaa
     2581 tcagcaataa tgagatttct tctgctactt tcagagtaga ataattgaca ttaaacaaca
     2641 taaaaaagcc cgacaaaaaa agtcgggctt ttttattttt actaccttta catcatatga
     2701 gaaaaatata cacacttatt agcctacatt ttttaatagc atctgctata gccggcccgg
     2761 gtacttatta taacagtttg gatacaacaa aatcatgtgc taattttaaa acacagctat
     2821 ataatttaat taaatccaac actacggtta tcccatatgc gcaagtagat aattatttta
     2881 attctacgga ttataaaccc tctgaaacag gaaccggaaa tgcgattatt gacaaataca
     2941 gttctgaaaa tccatttgga acagattatt gcaattttca atatccagca gatttttgtt
     3001 tgggacgatc ttcttctacg gagtgctttt gctatgacaa agaacacgta tttccaaaat
     3061 cgtggtttgg aggaactgat atgtacccga tgtattcaga cttgatgttt atatggcctt
     3121 ctgataatta tgtaaatttc agaaaaggca acctgccatt gggatatgta aattcaccgt
     3181 tttatacttc tcataacggc accaaaatcg gtacctctaa aacagcatcc aattataact
     3241 acagcaatac aaatgtattt gaacctatag attcttttaa aggtgatttt gccagagcat
     3301 atctgtatgt cattacacgt tatgaagatt ctattgtaaa ctggacaaac cgcagtattg
     3361 ccggaaatat gctggatggc aataaatatc caggattcaa atcctggctg ctgcaacttt
     3421 gcgtaaaatg gcataaactc gatccgccaa gtacccaaga gcgtagcagg aatgatgctg
     3481 ttcaatactt gcaaggcaac agaaatccat atatcgacca tcctgaatgg gcggaaaaag
     3541 tgttcggtcc aaacggactg tcagcgtctt gcgtgccaac tgcactttta caaaaacaaa
     3601 atttaagtgt agaaatattc ccgaatccgg taaaaaatat tttacatatt cgaaccgaaa
     3661 ataatgatac ctatcagcta acgcttaccg atattttggg tagaactgta tttaatcaaa
     3721 cagcattaaa tacaacattt gaaatcaata cccatgattt gccaaaaggg ttatacttac
     3781 ttcagctaaa ttctgaaaaa gcaaccacaa ccaaacaaat tattgtggag taactgcctt
     3841 cagaaaaaca acataggtag gaaagtgatc gctatagcca gcgttataaa tattgaaatc
     3901 ccaagtacgt ttaggataat ctttatattt cccggttttt tgtaccataa aatcacgctt
     3961 gaaaatgtag gctttgtgta aaaagtaacc gctttgcttg ttgtccagcc atcctttaga
     4021 aaatacttct tggtcaaaca atccccagtt atcgttaaat gccaatgtac ccaagccttt
     4081 tttggtataa tctacccatg gattataaaa tcctttgtct ttcgttttct ctcttttgct
     4141 tgaacaagcc aatacttttg tcatactcgg atcatcagga ttatcattta aatcgcccat
     4201 cacaatgatt tttgcgtcag gattgatagc cataatagaa tcagaaagtc gtttatcaat
     4261 tgcggcggca aattcgcgca aaggagcagt tgcctccgta ctgccacgtc tggatggcca
     4321 gtggtttaca aaaatatgca ccgtatctgt attgcctaat attcctttta catacaaaat
     4381 atctcgagta ggacgccaat cattcactac ttgctttaaa tctacagtta tagactggct
     4441 ttccagcact ttaaagtatt tcggattata aataaacccg caatctactc ccctcaaatc
     4501 cggtccgttg taatgtacaa tctgatagtt tctggttttt aacttaggat gattgaccag
     4561 tgtttccaaa accgacttat tctcaatctc agcacaaccc ataaatgcca agccatccgg
     4621 cgactggtct gtaccgatta aagacacaac ttcagctaga tgatttactt tatctttaaa
     4681 gatttcggcc gtataatgat aactgccatt gggcgtaaaa tcttcatcat ccacaccggg
     4741 ttgattaatt gtatcataga aattctccag attatagaac cctccgacga taatattata
     4801 ggtagttttt tgtccaaaaa cggaatttat catcagaaaa atccccgcac agaaaagcat
     4861 tttaattttc ataatcaaat gtacattttt gttgattaat tcatccctat ttgtcgtgaa
     4921 tttttagtga aaaagaactc gcatatctat ttttatcttt acttttacat agcaacaatc
     4981 aaaatcaata ccaacttttt ttttatgaat aaaaacatct atttgtctgc cttactgagc
     5041 acatgctttc tgttgggttt ttcaaaacaa gaggatgatt cactaaaagt gcaggcagga
     5101 gtaccagata cctcacgcac ctatcttgaa gttcctactg taaccttgga agacatggaa
     5161 acttctgatg aaagcggtta cagtcaaggc gtttctccag tactgaatgc gggtcgggat
     5221 ccttttattc aggctgccag tttttcctgg agtatttctc gttttaaaat cagaggatat
     5281 gatgccgatt attttgaaac atatgtaaac ggcataccta ctgaatatat tgataatgga
     5341 ttctctgcat ataatctgtg gtcggggttg aacgatgtta ctcgcaaccg cgaaaactca
     5401 tttggtatgc gggcatccac atttgcattc ggtaccattg gtggcttata tgcaattgat
     5461 atgcgtgcat ccaaacaacg caaacaaacc agtattacga ttagtgcttc aaacagaaca
     5521 tatgatatcc gtggcggtgc tacatggggt tcgggcatta ctaaaaaagg ctggagtttt
     5581 tctgcatctg tttttggaag atgggcaaaa caaggctatg taaaaggaac ttacatgcaa
     5641 agtttatctt atttcgcatc tatagaaaag ttttttaaac acaataattc attagcctta
     5701 actgcctttg gcgtgcccac aaaacaaggc aaagcggcaa atgtagttca ggaagcctat
     5761 gatttagccg gaacaaatta ttacaaccct aactggggtt tgcaaaatgg caaagtacgc
     5821 aatgcaagag tagaatatcg ccatcagcct gttataattt taacgcacga gtggaaacac
     5881 gattatggta atttgttaac tagtatcggc tattcatttg gcgaacgagc cttatccgga
     5941 attgacaaat acaaagctaa tgaccctaga ccggattact acaaatattt gcccagctat
     6001 caggatgatt cactacagga agccttggtt gcagagctat ggcaaaacag tgaatataca
     6061 agacaagtaa aatgggatga tatctatttt gccaataaaa atggagaaag tgttacgata
     6121 acgaatattg acggcgatgc cgctaaatct gtaaccggca aaagagcgca ttatatttta
     6181 ggggaagatg ttcaaaaata ccaacgtctt aatttcaata ctgtttataa tcacaccttt
     6241 aaaaaactag ctgatttaac ggcaggtgca tcttatcaat ttcagaaagt gattaatttc
     6301 aaacgagtga aagacctgct gggaggagat ttttatatgg atgtggatga atatattgag
     6361 tcggattcag tatccaaccc aagtgctgct tattccgatt taaacaaccc taatcatgcg
     6421 gtgtacaaag gcgataaata tgactataat tattcgtcca atatacatgt agcctccgtt
     6481 tgggcgcagc ctactttcaa attcaaacat atagatttct ttgtaggcgg caaactggaa
     6541 tataccaaac aatggcgtga agggtatttt aaaaacggat tgaacccgac caactcagaa
     6601 ggcgtttctc aaaaattcag ttatattgac tacggcgtaa aagcgggatt aaccgtgaaa
     6661 ataaacggta aaaattatat ctacgcaaac ggaatgtata ccactcgtgc accttattgg
     6721 gataatgtat tcatttcacc gcgaacaacg aacagcacct acaaccccgg cagtgaaaaa
     6781 atcggttctg ccgaactggg ttatatctta cattcaaata aactgaagtt gagagctaca
     6841 ttattttaca ccgaattctt aaacggaagc aacactattg tctattttaa cgacgatatc
     6901 tatacgatga tcaattacac gataagcggt attaataaaa gacattatgg cggagaactg
     6961 gcattggaag ctccattgta caaaggactg agcctgacat ttgctacctc tatgggacaa
     7021 tataaatata ccagcagaca aaagggtatt atgacggctg atgataatcc aagtttaaga
     7081 acagaagaag tcatctacag caaaaacttc aacgtggcaa acacgccgca aatggcctat
     7141 aatttgggtc tgttttacag gagtaaaaaa ttctggtata taggtgccgg ggtgaatttc
     7201 tttgactgga tatatactga ttttgctcct actcaccgaa cggataatgc aaccgatgct
     7261 gttccttatc actctgactt atggtattct atcatcaatc aggagagaac atcaccaaaa
     7321 ggacaatgga cactagatat gagcggcggc tattcgtgga gattaaaatc aacctttaaa
     7381 aacatgaagg gcaagtatgc caataaatat tatttagtca tcaatgcggg actgtcaaac
     7441 atcaccaata ataaaaaatt catcgtaagc ggcagagaac aaatgcgatt tgattatgct
     7501 gaaaaagacc cgatgaaatt tgctcctaaa tacagctatg cctacggaat aaacttttat
     7561 gccaacttaa cattcagatt ctaaaaaata aatactcata aaaaataaaa tagcaacaat
     7621 gaatacaacc atgataaaaa gatttttaat agcgtttgta gccattacag ccataacgct
     7681 cagctcgtgc aagaaagaga aatatgatga accgagtggc aatacaccgg atccgaattt
     7741 gacaacaatt acaatagaca gcttgcgtaa atattttacc agtggtttgc ctattactat
     7801 taccaatgat gtaacgatta gtggaattgt aaccgctgat gacaaagacg gcaactttta
     7861 caaacaaatt gtaattgatg atggaacggc aggtattcct gtactgatag aaagaagtta
     7921 tttgtatacc gattttcctc aaggaagaaa aatatatgta aaatgtaaag gattggtatt
     7981 gggagcttat cataactatt tgcagcttgg cggctttgtt gacaattcta ccgggcagcc
     8041 tgcggttgga aatattccgt cagatttggc tgacaaagta attgtaaaag gcccgacagg
     8101 aaatgcggtt acacccatag tagttaccta ctcacagcta aataactcat atcaagcaag
     8161 attaataaaa atccaaaata cagaatttga agaagctgca gtaggattac catatgcgga
     8221 tataattaat caacaatcta tctcaagaaa tttgaatgtt tgcgatacat tacctgggac
     8281 agatccaaag atagaagtaa gaactagcaa ttatgcactc tttgcaaatg agctcgtccc
     8341 agcaggtcat ggtgatttta taggtgtata tagtgtttat aatacttcta agcagcttac
     8401 tattcggaat ctttcagatt ttgattttag tggtggtaca agatgtaatc caataacggt
     8461 ttatagccct gcagatattt ctacattaag aaatatctac acaggtagta atgtagctgt
     8521 aggttcagac cttctaatta ctggtgttgt aatttcttca atagctgaca gtaataattt
     8581 agcacaaaac ttaacaatac aggataatac aggtggaata gtagtgcgta tggcatcttc
     8641 tagtcctaat tttaataaag gtgatcaagt tcaagttaag attggcaatg cagatatctt
     8701 acaaagattc agtaatggtt cattacaaat cggaactatt caaaatgcaa aaattactaa
     8761 aattgcttca aatgtaaatg ttacacctca aacagttaca attgcaacgc tcatttctaa
     8821 ttttaatgat tatgaatcag agttaataaa agtaactgga gtaacaatct caggtggtag
     8881 tggactatat tccggaacat taacatttac agatgcaaca ggcagtatag catcatttgt
     8941 gcgaacggcg actatggttc ctagtgctga atttgtaaac caatcttatc ttacaagtac
     9001 agttaatatt gttggatatg caaacttaaa cggtagtact aaacaactta tcctgagaaa
     9061 cgctagtgat gttactccat agacattgat attaataaaa agaataggct gtcttaaaag
     9121 ttaaaataaa ggctctgagg ttttaaaatc ctaaattttc tcagatacgt atccaaataa
     9181 aaagaggctg tttcaagtat tgagacagcc tcttttttta tatgtgttcc ctacttccta
     9241 actgaaaaat agcatacatt aaatcatatc aatgaattat aaacaaaacg agtcaaaatt
     9301 attggctaat acttgagttc aagttaatat tctctattag aaattacaga ttttattatt
     9361 atcaaactaa ctcttatttc ataataatga tggtgtgtgg catgtagcat tgtcttattc
     9421 taattgttta ggtgaagcta ataatctgaa atcaactttg ttttttattg caatttatct
     9481 caatcccagt caaaataaaa tgcagtattt aaagtaagat atgcatttca caaaaaaagg
     9541 caaaggcagc acatggtgta ctgcctttgc caaagtagca tgaaatgaga aagagttgtt
     9601 tttctatagg tctatgctta atgtctgata agattcgccg tgttgtgtga tatctaagcc
     9661 cagttcttct tcttctgctg acacacgcat cggctgtatt ttgtcggtaa taaaatataa
     9721 gatataagaa cccgtaaaag caaatgctgc aacaccaatc aaggcgataa tatgtacggt
     9781 aaaggtatga tataccccat acatcaagcc ccaatcggca gtttttaatc ctgttccgta
     9841 atcggcagca aataccccgg taaaaaacat ccccataatg ccgcccacac catggcaagg
     9901 aaatacatca agggtatcat ctaaggtaga tttatttttg taatgtaccg caaaattgct
     9961 cactaaggca gaaagtgccc ccacagcaat actctctcct acatttacat atccgcaagc
    10021 aggagtaatg gctaccaaac ccaccacagc ccctatacaa gccccaatgg aagatacttt
    10081 atagccgcgc aaagcatcaa caaataccca catcagcatg gcagcagcag cagcagtatt
    10141 ggtagtggca atggcattcg ccg
//
LOCUS       DAOQII010000068        10151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3455_length_10151_cov_5.091026, whole genome shotgun sequence.
ACCESSION   DAOQII010000068 DAOQII010000000
VERSION     DAOQII010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3455_length_10151_cov_5.091026"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PLF48_04790"
     CDS             complement(<1..1211)
                     /locus_tag="PLF48_04790"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF48674.1"
                     /translation="MQTIKNQTLKNNVLQKPYRASTNFWLSDKMLRNYFEQHVKQDAQ
                     SFMLPFLYNTGELAAGEMNAWSLTADKQTPVLVKRNYWGETINKIEFHPDYFKLLDVA
                     VKSHMFTVKWKPGYRNKFNGQLHLLGFLSGFLFAQTESSQYCPLCMTDGAARIIDRFA
                     NDADRERLLEHIFTQNYLDLYTGAMFLTEKTGGSDVGANIVEAHHVQDDIYTLKGEKW
                     FCSNASAEIKLVLARTNPEIKGTRGLSIFLVEQYLENGERNPLDIVRLKDKLGVRSMA
                     SGECIFENTQAKLIGKEGEGFKIMTEMINLSRLYNSVAAIACMRRALHEAYQYVQQRI
                     TFGKYTIEHAMVQEKLMELGLLYQQNFYLTFRSIAALDAADNGNETESGLVRLLTPMT
                     KKFTAEKSVYL"
     gene            complement(1389..1907)
                     /locus_tag="PLF48_04795"
     CDS             complement(1389..1907)
                     /locus_tag="PLF48_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF48675.1"
                     /translation="MLLIMKDSVVSVDYELKVDDGNGNLITADKSQPGQPLVYLHGSG
                     QLLAEFEKNLLGKTAGDTVSFTVSAENGYGTRRDEDIVEIPVDSFKGQDGNLDTTVVK
                     VGNVLPMMDNQGNQFQGIVVDVNDQFVVMDFNHPMAGKELNFTVTVLNVRPATAEELA
                     HGHVHGEGGHHH"
     gene            2164..2889
                     /locus_tag="PLF48_04800"
     CDS             2164..2889
                     /locus_tag="PLF48_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48676.1"
                     /translation="MDSTKKFQEAFDTLLKDFNSKIEEVRSNIQNLTADLKVQLEGQR
                     ENLQEYGEKIKGRINEIVDVDKVRSNVLAEAENLVDDAKEKVEKAFQFINEQVNTGEE
                     KAKARAEKFAKEAEKVSNSLEKEVKKAAKDFQKSVEKVTAKVEKDFNKARKEVETKVE
                     KVAKDVKASAAKAEKTVKADAGKVAKKVDTTVKKVAETAKKATTTAKKATTPAKKTAT
                     TAKKATTTAKKAATKTTKTASKK"
     gene            complement(2955..5672)
                     /locus_tag="PLF48_04805"
     CDS             complement(2955..5672)
                     /locus_tag="PLF48_04805"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF48677.1"
                     /translation="MFKKTQLAAAILIWSCITFAQNGSLVFNTEVEGVKQFTLKENGL
                     RVLLIPDKTAGNVVVNIVYEVGSRHEGYGESGMAHLLEHMLFRSCKNFVNIKQAIAEK
                     GAMANGTTSYDRTNYYEILPASDSNLNWALAMEADRMVNSKILQEELNTEFSVVRNEF
                     EMGENDPDAILNERILSTMYLWHNYGKSTIGSKEDIERVTADRLKAFYQKYYQPDNAV
                     LIVAGKFDEEKAKQYIVQNFASIPKPQRILQPTYTVEPPQDGIRYVELKRIGDMQSIQ
                     WAYHTPALAADDYAANDVLFSILTDNPNGILYKRFVETKLANSLSGYTFSNRDPGASM
                     YDLILPKTADMALVEKELFKILNGMDTLTITQHDVDLAKSSTLKFWNNITDNTQRFST
                     FITDIIGAGDWRLWYKYRDDVEKVTLADVKKVIQNYYKPSNLTWGKFIPDKNPDRAIV
                     PEIKDIDAIVANYKGRETTKMTESFSTDYDSILAKVKSNKTKNGLKYILLPKPTKSDV
                     ILFSVDLRFGNETALQNTQGINNVLASLMEEGTTTQSKEKIAEMLNKLKSEISFSGRA
                     SSLSINVKTTKANFSACMDILKDMLMNPKLSEADFQKIIQQNISNIEESMNDPEARAQ
                     EKLDRLTTAYPSSHPFYASTFEEQLAYYKNMKLSEVKAFYDKFYGANYGIGVVVGALN
                     ETAVAKAMENIFGKWNSKTSWQKINDKYFDVKQQTVTVNTPDKTNAVCSGNINLNISM
                     QHPDFPALYMADYLLGGGAFLSSRIAQRLRENEGMSYGAGSYVYADYNNNFGNWGMYA
                     IFNPAYKNRLDSALLDEIHLALDKGFTKDELEKAKIAWMQLQNSRLGTDNSLKSLFMG
                     YLDVNRDLKDRKVFQDKVKALTIEQVNAALRKYFDLTKLVLVYAGDFKH"
     gene            5809..7194
                     /locus_tag="PLF48_04810"
     CDS             5809..7194
                     /locus_tag="PLF48_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNF48678.1"
                     /translation="MKTTISTSGIAQLVQEQRTYFESGSTRNVDFRIQQLKRLKNAIK
                     AHEDEIYDALYADFRKSKFETYATEIGVVYEEINCMIKNVKKWSTPELVKDTLANFPS
                     KNYIYPEPYGVCLVIGAWNYPFQLTLAPVVGAMAAGNTCIIKPPRAALNTHHLIYKII
                     EDNFSTQYLTVLEEHSDNNEMLAQRYDYIFFTGGVEIGKTIARAAAEYLTPVTLELGG
                     KSPCVVDKNSDVEVAAKRVAWGKFLNGGQTCVAPDYLLIHEDVKDKFYSAFANSVTSF
                     YGNNIQESTDYPRIINDKHFKRLAAMIVDGNIITGGQTDEETRYIAPTLIEIDDIEHP
                     LMQDEIFGPILPAISIGSMEEAIAIIKRFEKPLAFYLFSNSYDTQQQFIQTIQFGGGC
                     INDTISHFINDQLPFGGIGNSGIGAYHGKYSFDTFTHKKPVCNKVTWPDVPLRYPPYN
                     AKLPIIKQVMK"
     gene            7237..7572
                     /locus_tag="PLF48_04815"
     CDS             7237..7572
                     /locus_tag="PLF48_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HNF48679.1"
                     /translation="MENKIKMNELGEFNKNDAQLWEMAQKRAKFKRHAMIYALVIGFI
                     WLAKAFGLMWFTPGLSFVTIIWGAVIGYQYWQTYHGDEQTMVQKEFENLKREKGDVTS
                     YSTERKDIH"
     gene            complement(7579..>10151)
                     /locus_tag="PLF48_04820"
     CDS             complement(7579..>10151)
                     /locus_tag="PLF48_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNF48680.1"
                     /translation="LLLDLHEIHQRVAEYNQPLEKFLPWGKLILKDFDECDKYLVDTQ
                     KLFSLLNEQKSLEDFSFLEEELRTYIEQFLLTTLTIKNQEETYKQHFIKTWRLLGEIY
                     THFQQKLNNEKKAYEGMMYKDVLQQLESSKLILSYSQITFVGFNALTLSEEKIFKTIA
                     KSYTANFYWDVADDFMENKHHEAGIFMRLNKHVFHTKNNHWINQQAIQKEVTICGIAS
                     DVGQAQYAVSLLCEDKHKQVALVLCDEQLLHPLLYHVEADQCNITMGYPARYHEVFAF
                     TTQLIQFYSRARLREQQTDFYYRDVLGLMQSVFLRPKIRDLEKLQATASYFVPYFPEN
                     YIRDFFPDFLFIREYSFTAILHQVIAIIQSSEFDDAYFIPVREAIIESLSSFVDLIAR
                     KQLELSREILPQLYRQYFSGIKIPFETKTESRVQIMGFLETRLMDFDEVIILSANDDK
                     LPGSHKNNSFIPYNLRKGFGLPTYEQADGIGAYHFYRLLKRAEKITLLYNNKSGEDSS
                     EKSRFIRQIEHEWTSKAGTLKHKVILPETKTDLPPEQEQPIEIKKTDAMKVQLAERVY
                     SPTALKLYIKCPLQFYLRYVEKIKEPEQSDDDFDDGTFGLILHKTLELIYKPYIGKTI
                     SEENIIAFSSVLFIRKKIKEACESEDLKLPKEILSSRHQLQFKVIETYVKRILVNDAK
                     EKKLQVLHTEQKFKWENLPLENGTFVVLEGIFDRVDKMDENTIRIIDYKTGVIHLPDF
                     PNEDEQPDKFLESLFTFTDKNHDYSAAFQGLLYALMYYKLFNCTHIIVAYHHTRTITS
                     GLSYLNEGKPIPVDLLLSFEKRLSDLLSEIIYKQDSFIQSENKNAYDYSPYADMLGVN
                     "
BASE COUNT         3138 a         1978 c         1781 g         3254 t
ORIGIN      
        1 ataagataaa cactcttttc ggctgtaaat tttttagtca tcggagtaag cagacgcacc
       61 aatcctgatt ctgtttcgtt gccgttatca gcggcatcta aagccgctat tgatctgaac
      121 gtgaggtaaa aattttgctg atatagaaga ccaagctcca tcaatttttc ttgaaccatg
      181 gcatgctcaa tcgtgtactt tccaaaagtg atgcgttgtt gtacatattg ataagcttca
      241 tgcaatgcac gacgcataca tgctatggcg gccacactgt tatataaacg cgaaaggttt
      301 atcatttccg tcatgatttt gaacccctct ccttccttgc caattaattt agcttgtgta
      361 ttctcaaaaa tgcactctcc gctagccata ctgcgcactc ccagtttatc cttaagcctg
      421 acaatatcca aaggatttcg ttcaccattt tccaagtatt gttccactaa aaaaatactc
      481 aatccgcgtg ttccttttat ttcgggatta gtacgagcca aaaccaattt tatttctgca
      541 ctggcattac tacaaaacca cttttcgcct ttaagcgtat agatgtcatc ttgtacgtga
      601 tgggcttcta caatatttgc acccacatcc gatccgcctg ttttctcagt aagaaacatg
      661 gcgccggtgt acaaatccag ataattttgg gtgaagatat gttccagtaa acgctccctg
      721 tcagcgtcat ttgcaaaccg gtcaataata cgtgccgcac catctgtcat acataacgga
      781 caatattgac tgctttcagt ttgtgcaaat aaaaaacctg acaggaagcc taacaaatgt
      841 aattgaccgt taaatttatt tcgatacccg ggtttccact tcaccgtaaa catgtgagat
      901 ttcaccgcca catccaacaa cttgaaataa tccggatgaa actcaatctt gttaatcgtt
      961 tctccccaat aattgcgttt tactaaaacc ggagtttgtt tatctgcggt taaagaccat
     1021 gcattcattt caccggctgc aagctctcca gtgttataga gaaatggcaa catgaacgac
     1081 tgagcatcct gcttgacatg ttgttcaaaa taattccgca acattttatc gctcagccaa
     1141 aaattggttg atgctctgta cggtttttgc agtacgttgt tttttaaagt ttgatttttt
     1201 atagtttgca tgatgctata attgtaagta aaagcagtag taatattcag ataaaaaaac
     1261 ataagcattt tcaatgctgg tttactaccg tatccatctg gatttttata ttcttttaca
     1321 atttatgatt atgtaagcag actttttaca aactcatcaa cattcaacac ttggaattta
     1381 tcattctatc aatgatggtg tccgccctct ccgtgtacat gtccatgtgc cagttcttca
     1441 gcagtagctg gacgaacatt taaaacggta acagtaaaat ttaattcttt tccagccatc
     1501 gggtgattga agtccataac aacgaattga tcatttacat ctactacaat accctgaaac
     1561 tgattaccct gattgtccat catcggcaat acattgccca cttttacaac tgtagtatca
     1621 agatttccgt cttgtccttt aaagctgtca accggaattt ccacaatgtc ctcatctctt
     1681 cgtgtgccat aaccgttttc agcagatact gtaaaactca ctgtatcacc tgctgttttt
     1741 cccaacagat ttttctcaaa ttcagccaat aactgacctg aaccatgtaa gtaaaccaaa
     1801 ggttgtcccg gctgggattt atcagctgta atcaagtttc cgttgccgtc atccacttta
     1861 agttcgtagt ctacactcac tacgctgtct ttcataatta ataacatatt gcttattttt
     1921 ttcacgaagt tagttaaatt ctatcagaaa gcagcacaaa gcatctgaat ggagtcaact
     1981 aaaatttaca gtttttaaaa tataataata tataattaat atacaaaaac cacttaactt
     2041 caaattctca ttggcgtata agtaatttaa aatcaatttg tttttattca gaatagttta
     2101 atgtggcaat aactgtcaat taatccaaat gaaataattt tgtacaaata aaaatataat
     2161 atcatggatt caacaaaaaa atttcaagaa gcatttgata ctttactaaa agatttcaat
     2221 tcaaaaattg aagaagtacg cagcaatatc caaaatctta ctgctgactt aaaagttcag
     2281 ttagaaggac aacgtgaaaa cttacaggaa tatggcgaaa agataaaagg gagaattaac
     2341 gaaattgttg atgtagataa agttcgctca aacgtgttgg ctgaagcaga aaacctggtg
     2401 gatgatgcta aagaaaaagt agaaaaagca tttcaattta tcaatgaaca agtaaatacc
     2461 ggcgaagaga aagcaaaagc aagagctgaa aaatttgcta aagaagctga gaaagtgtct
     2521 aactctttgg aaaaagaagt aaaaaaagca gccaaagatt ttcaaaaaag cgtagaaaaa
     2581 gtaacggcta aagtagaaaa agactttaat aaagccagaa aagaggtaga aactaaagtt
     2641 gagaaagtag caaaagacgt aaaagcatct gctgctaaag ctgaaaaaac agtaaaagct
     2701 gatgccggaa aggtggctaa gaaagtagat actacagtaa aaaaagtagc agaaacagct
     2761 aaaaaagcaa ccacaactgc taaaaaggcg actacgccgg ctaagaagac agcaactact
     2821 gcgaaaaaag cgactactac tgcaaagaaa gctgcaacta aaacaacaaa aactgcatct
     2881 aaaaaatagg atttatttgg ttagatattt tctgatgcgg ggtgcgccaa aggcgcaccc
     2941 cgcatctttt tttatcaatg cttaaaatct ccggcataaa ctaataccag cttagttaaa
     3001 tcaaaatatt ttctcagtgc agcatttacc tgttctattg tcaatgcctt tactttatcc
     3061 tgaaatactt ttctgtcttt caaatcacgg ttaacatcca gatagcccat aaataatgac
     3121 ttcagtgaat tgtctgtacc gagcctgctg ttctgtaatt gcatccatgc tatttttgct
     3181 ttctccaact catctttggt aaacccttta tctaaagcca gatgaatttc atcaagcaat
     3241 gctgaatcta aacggttttt gtaagccgga ttaaagatgg catacattcc ccaatttccg
     3301 aagttattat tataatctgc atagacataa gatccggcac catagctcat gccctcattt
     3361 tcgcgtaagc gttgggctat tctcgaagat aaaaaggcac ctccacccaa taaataatca
     3421 gccatataga gggcaggaaa atcaggatgc tgcatagaaa tattcaagtt gatattgccg
     3481 gaacatactg catttgtttt atcaggagta tttactgtaa cggtttgctg ttttacatca
     3541 aaatatttat cattgatttt ctgccagctc gttttactgt tccatttccc gaaaatattt
     3601 tccattgctt ttgccactgc tgtttcattt aatgccccaa ctacaacgcc gataccgtaa
     3661 ttagctccgt aaaacttatc gtaaaatgct ttcacttcac taagtttcat gtttttgtaa
     3721 taagccagtt gttcttcaaa agtagaggcg taaaatggat gtgatgacgg ataagcggta
     3781 gtaagtctgt ctaacttttc ttgtgcccga gcctcaggat cattcataga ctcctctata
     3841 ttgctgatat tttgctgaat gattttttga aaatccgctt cgcttaattt tggattcatc
     3901 agcatatcct ttagtatatc catacatgcg gaaaaatttg cttttgtcgt ctttacattt
     3961 atagataaag aacttgccct gcctgaaaaa ctaatctcag attttagctt gttcagcatt
     4021 tctgcaattt tttctttgct ttgtgttgtc gtaccctctt ccattaaact tgccagcaca
     4081 ttatttatgc cctgtgtgtt ttgcaatgct gtttcattgc caaatcgcaa atcaacggaa
     4141 aacaatatca catcggattt ggtaggcttg ggtaatagaa tatattttaa tccattttta
     4201 gttttgttgg attttacttt tgccagaatg ctgtcataat ccgtagagaa ggattccgtc
     4261 attttagtcg tttctcttcc tttataattt gctacgatag catcaatatc ttttatctcg
     4321 ggtactattg ccctgtccgg atttttatcc ggaataaatt ttccccaagt caagttggag
     4381 ggtttgtaat aattctgaat cacttttttt acatctgcca aagtaacttt ctctacatca
     4441 tcgcggtatt tataccacaa acgccagtca cctgcaccaa taatatctgt aatgaatgta
     4501 gaaaaacgct gtgtattgtc ggtaatatta ttccagaatt tcaaggttga acttttagcc
     4561 agatccacat cgtgttgggt aatcgttagc gtatccatgc cattcagaat tttaaaaagc
     4621 tctttttcta caagtgccat atctgctgtt ttaggcaaaa tcaaatcata catggaagct
     4681 ccggggtcac ggttggaaaa agtatagcct gataagctgt tcgctaattt tgtttccaca
     4741 aatcttttat ataaaatgcc attgggatta tccgtaagta tgctgaacaa cacatcgttg
     4801 gcagcataat catctgccgc cagtgccgga gtatggtatg cccattggat agactgcata
     4861 tcaccaattc ttttcaattc tacgtatcgt atgccgtctt gtggcggctc aacagtatat
     4921 gtaggttgta aaatgcgttg aggtttcggg atggaagcaa aattttgtac tatatattgc
     4981 ttggcttttt cctcatcaaa ttttcctgca acaattaaaa cggcattgtc gggttgataa
     5041 tatttttgat aaaatgcttt aagcctgtca gccgttacgc gttcaatatc ttctttagaa
     5101 cctatcgtcg atttaccgta attatgccat aaatacatag tagacaagat acgttcattc
     5161 aggatggcat cagggtcatt ttcgcccatt tcaaattcat tgcgcactac gctaaattct
     5221 gtattcagtt cttcttgcaa tatcttggaa tttaccatcc tgtcggcttc catggctaat
     5281 gcccagttga gattagaatc tgatgcaggc agtatttcgt agtaattggt tctgtcgtat
     5341 gatgtggtac cgtttgccat tgcacctttc tcagctatag cctgtttaat gttgacaaaa
     5401 tttttgcaag aacgaaaaag catatgttcc agtaaatgcg ccatacccga ttctccatac
     5461 ccttcatgcc gagagccgac ttcatacaca atattgacaa ctacatttcc tgcggttttg
     5521 tccggaatga gcaatacgcg caatccgttt tctttaagcg taaactgctt tacaccttcc
     5581 acttcggtat taaagactaa acttccattt tgggcaaacg tgatgcaact ccaaatcaga
     5641 attgctgctg ccagctgtgt ttttttgaac attttttttg tttatttatg cgaatataac
     5701 aaaatctaat tcgtggatga tgttaaaaat cctaattaaa aggaatgata ctggtgctaa
     5761 ttagcttgtg taaagtagca ttacatctaa ttttagtatt tttagcttat gaaaactaca
     5821 atttccactt ccggcatagc tcagctcgtg caagagcagc gcacttattt tgaatcgggt
     5881 tctacacgaa acgttgattt cagaattcaa caactaaagc ggctaaagaa tgctattaaa
     5941 gcacatgaag atgaaatata tgatgcattg tatgccgact ttcgcaagtc taaatttgaa
     6001 acatacgcca cagaaatagg agtagtgtat gaagaaatca attgcatgat aaaaaacgta
     6061 aagaaatggt caactcctga actggtaaaa gatacccttg ccaatttccc gtctaaaaac
     6121 tatatctatc cggaaccata cggcgtatgt ctggttatcg gtgcatggaa ctatcctttt
     6181 caattaactt tagctccggt tgttggtgct atggcagcag gtaatacttg cattatcaaa
     6241 ccacccagag ctgcattaaa tacgcatcat ctcatttata aaattattga agataatttc
     6301 agtacgcaat acttaactgt actcgaagaa cattcagata ataatgaaat gctggcgcaa
     6361 cgctatgact atatcttctt tactggaggg gttgaaatag gcaaaacaat tgcccgtgct
     6421 gccgcagaat atctgacgcc tgtaaccttg gaattgggcg gaaaaagccc gtgtgtggta
     6481 gataaaaaca gcgatgtgga agtggcggca aaacgtgttg cctggggaaa atttttgaat
     6541 ggcggacaaa cttgcgttgc accggattac cttctgattc atgaagatgt aaaggataaa
     6601 ttttattcgg cttttgcaaa tagtgtaacc tcgttttatg gcaataatat tcaggaaagc
     6661 acagattatc cccgcatcat caatgataaa catttcaaaa ggttagcggc tatgatagtt
     6721 gacggtaata ttattaccgg cggacaaaca gatgaggaaa cccgctatat tgcacctacg
     6781 ctcattgaaa tagacgacat agaacatcct ttgatgcaag atgaaatttt cggccccata
     6841 ttgcctgcca tttctattgg ctctatggaa gaagctattg ccatcatcaa acgttttgag
     6901 aaacctctgg cattttatct cttttccaac agttatgata ctcagcaaca gtttatccag
     6961 actatacagt ttggtggtgg ttgtattaat gatactattt ctcattttat caacgaccag
     7021 ctaccttttg gcggtattgg caatagcggt atcggcgctt atcatggcaa atacagtttc
     7081 gataccttta cacataaaaa accggtttgc aataaagtaa cctggccgga tgtgcctttg
     7141 cgttatccgc cctacaatgc caaattgcct attatcaaac aagtgatgaa ataatttttg
     7201 atttcaggga attttttgga gagattttgc gttataatgg aaaacaaaat aaaaatgaac
     7261 gaactgggag aatttaataa gaatgatgcg caactttggg aaatggctca aaaacgagcc
     7321 aaatttaaac gccatgccat gatatatgca ctggtaattg gtttcatttg gctggcaaaa
     7381 gcttttggct taatgtggtt tacgccgggc ttaagttttg ttaccataat ttggggtgct
     7441 gtaatcggtt atcaatactg gcaaacttat catggcgacg aacaaaccat ggtgcaaaaa
     7501 gaatttgaaa atctaaaacg tgagaaaggc gatgttacat cgtactctac tgaacggaaa
     7561 gatattcact aatcattact aatttacacc aagcatatct gcgtacggcg aatagtcgta
     7621 cgcattttta ttttcagatt gaataaaaga atcttgttta taaataattt cactcagcaa
     7681 atctgataat cttttttcaa atgacagcaa taaatcaaca ggtataggtt tgccttcgtt
     7741 gagataactc agtccagaag tgatggttcg ggtatgatga taagcgacaa taatatgggt
     7801 gcagttaaat aatttgtagt acatcagtgc gtataaaagc ccctgaaatg ctgcactgta
     7861 atcatgattt ttatctgtaa acgtaaagag actctctaaa aacttgtcag gctgttcatc
     7921 ttcattagga aaatccggca agtgaattac accagtttta taatctatga tacgtatcgt
     7981 gttttcatcc attttatcta cacggtcaaa tattccttcc aagacaacaa aagttccatt
     8041 ttccaaaggt aaattttccc atttaaactt ctgttccgta tgtaatacct gcaatttttt
     8101 ttctttggca tcattaacta atatgcgttt cacataggtt tctatcactt taaattgcaa
     8161 ttgatgccga ctgcttagaa tttctttcgg cagtttcaaa tcttccgatt cgcatgcttc
     8221 ttttattttt ttacgaataa ataaaacaga tgaaaatgcg atgatattct cttcagaaat
     8281 cgtctttccg atatagggtt tatagataag ttctaatgtt ttatgcaaaa taagtccgaa
     8341 tgtaccatca tcaaagtcgt catccgattg ttcgggctct ttaattttct ctacatagcg
     8401 cagataaaat tgcaaagggc atttgatata caattttagt gcagtaggtg aatatacccg
     8461 ttctgccaat tgtactttca ttgcatctgt tttctttatt tcaatgggtt gctcctgctc
     8521 aggaggtaag tccgtttttg tttccggcag tatcacttta tgcttgagcg tacctgcctt
     8581 agatgtccat tcatgttcta tctgtctgat aaaacggctt ttttcactgc tgtcttcgcc
     8641 acttttattg ttgtaaagca aagtaatttt ttctgcacgc ttcagcaatc tgtaaaagtg
     8701 ataagcacca atgccatcag cctgttcgta agttggcaag ccaaaacctt tgcgcaaatt
     8761 atagggtata aaagaattgt tcttatgact gccgggcaat ttatcgtcat ttgctgaaag
     8821 tataataact tcatcaaaat ccatgagtcg tgtttccaga aaacccataa tttgcacccg
     8881 gctttctgtt tttgtttcaa atggaatttt aatgcctgag aaatactgac gataaagctg
     8941 cggtaaaatt tcacgggata attctaactg cttacgtgct atcaaatcga caaatgaaga
     9001 tagtgattct ataatcgctt ctcgtaccgg aataaaatag gcatcatcaa attcagatga
     9061 ttgtataatg gcaataacct gatgaaggat agccgtaaat gaatattccc ggataaacag
     9121 aaaatcggga aagaaatccc ggatataatt ttccggaaaa tagggtacaa aatagcttgc
     9181 tgttgcctgt agtttttcta aatcacgaat tttaggtctc aaaaatacag attgcattaa
     9241 ccctaaaaca tcacggtaat aaaaatctgt ttgttgttca cgcagtctgg ctctcgaata
     9301 aaattgtatg agctgtgttg taaaagcaaa cacttcatga taacgagccg ggtagcccat
     9361 ggtaatattg cactggtctg cttctacatg atataaaagc ggatgcagca attgctcatc
     9421 gcacaacact aaagcaactt gcttgtgttt atcttcacac aaaagcgaaa ccgcatattg
     9481 agcttgacct acatccgacg caataccgca aattgtcact tctttttgta tcgcttgctg
     9541 atttatccag tgattgtttt ttgtatgaaa tacatgctta ttcagccgca tgaaaatacc
     9601 ggcttcatga tgcttatttt ccataaaatc atctgcaaca tcccaataga aatttgcagt
     9661 ataagatttt gcaattgtct taaatatttt ctcttcactt aatgtcaaag cattgaagcc
     9721 gacaaaggta atttgtgaat aagacagtat cagtttactg ctttctaatt gctgcaacac
     9781 gtccttgtac atcataccct cgtaagcttt cttctcatta ttgagttttt gctgaaaatg
     9841 tgtgtagatt tcaccaagca aacgccatgt tttaatgaaa tgctgcttat aggtttcttc
     9901 ctgattttta atagttaatg ttgtcagtaa aaattgttca atgtaggttc ttaattcttc
     9961 ttccagaaat gaaaaatctt ccagcgattt ttgttcgttt aaaagagaaa acaatttttg
    10021 cgtatccact aaatacttat cgcattcatc aaaatctttc agtatcagtt ttccccatgg
    10081 caaaaacttt tccaaaggtt gattatactc tgctacacgc tgatgtattt cgtgcaaatc
    10141 caacaataaa t
//
LOCUS       DAOQII010000069        10101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3485_length_10101_cov_5.873681, whole genome shotgun sequence.
ACCESSION   DAOQII010000069 DAOQII010000000
VERSION     DAOQII010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3485_length_10101_cov_5.873681"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1596)
                     /gene="lysS"
                     /locus_tag="PLF48_04825"
     CDS             complement(64..1596)
                     /gene="lysS"
                     /locus_tag="PLF48_04825"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNF48681.1"
                     /translation="MQQLSEQEIIRREKLEKIKELGINPYPAALYPVNAYSAEIKENY
                     KEEILEDGTKNRLNFQEVCIAGRLMATRPAGKATFAELQDSQGRIQIYIRRDDICPGD
                     DKSLYDTVFSKLMDLGDFIGIKGYAFKTKVGEISVHATELVLLAKSLHPLPVVKVDSE
                     GNTHDAFTDPELRYRMRYVDLIVNPQVKDTFLKRTKMMNSIREFLNEKGALEVDTPVL
                     QSIPGGAAARPFKTHHNALDIPLYLRIANELYLKRLLVGGFDWVYEFSRNFRNEGMDR
                     THNPEFTVLEFYVAYKDYFWMMETTEQLLEKMAMDVHGTTFVEVGENMINFKAPYKRI
                     SIYDAIKTYTGFDVNGMDETALRDVCKQLNIEVDNTMGKGKLIDAIFGERCEHNYIQP
                     TFITDYPIEMSPLTKKHRSKEGLVERFELMINGKEMANAYSELNDPIDQRERFEEQVK
                     LMERGDHEAMYIDYDFLRALEYGMPPTSGIGFGIDRLAMLMTNNASIQEVLFFPQMRP
                     EQ"
     gene            1708..3702
                     /locus_tag="PLF48_04830"
     CDS             1708..3702
                     /locus_tag="PLF48_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48682.1"
                     /translation="MYRIFLFLILLLVLQANAKETKFKFGSVSKEELEMKNYSSDTSV
                     GAVVLFDVGSISYEVSERMSGHYVSYSFKQVFNRKLRIKIFDKKNLDMANQKMVLYLG
                     AQSDNFITKKKAHTINFENNKQAEIEVNKDELFLDRIFDNYYMLKIPFKNVKEGSIIE
                     MDITIESPLNYKLPTWYFQREIPTLFSQFSVSIPEFYKFRKNISGYLAINPPVSEIED
                     ITLNSQENVQSNKTTWTAEKVPAFIREPYLDCADNYLSKIEYELSSVQFPLQLEQPLS
                     MNWKEVNKMLNDAEYLGKQITTSSGFMKDMYDELKSKDNLSKLELMKAAYYAVKKKMR
                     FNQVYSLASLDGTKEAYRKGEGNSADINLILLKLLRDLNFNVAAIAVSTKTNGNVSYF
                     PSLFAFNQLIVQVTLEGKKYLLDATEKAYPMHLLPEEDLNDRGLIISDQNIEWVDLRT
                     NIPSGITLHAIDAKITNEGTISGKITIQRKEYAAANFKEKHNDKVDIDQYTKKLEEDN
                     LIELQDKNIQNLDSVEKPIIETYTFVKQNQTELGAANMLYVNPFIIDKLSVNPFKLNE
                     RDYPVNFNHPFDETYIINLSLPEGYAVSELPANASFSLPDNSGKLTYKITASAGNTIQ
                     LNCKFSITKSLFSFDEYPNLKEFYNQYLGKLNEMIVIKKI"
     gene            3706..5574
                     /locus_tag="PLF48_04835"
     CDS             3706..5574
                     /locus_tag="PLF48_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNF48683.1"
                     /translation="MINNKLLEIYAAIFFAFCFNNSFADKTEPVNAVIEHYDTKMSID
                     DKGYKIHVYKKIVLYNDNAIDETRIQLYHSGMDDSKLIKAEVRDLSDKLIRKIKSSDL
                     NDYGDFSNFSSDAHVKFYEFSDLTFPCTIETEYEYSARYLLYFPFWEPVTDFNVLVKD
                     ASLQISVEGKTGLRYKELNMPKTVEVNLVGGVKTYTWKLVDFKPLKEEKYIDEMSLPF
                     PKVLLAPSELNYNSYSGKFETWSDLGNWILLLNAQRDNLDEQTTLKVKEIAAKYPDDL
                     SKMKALYEFMQNTTRYVSIQYGIGGFQPFPASYVAEKGYGDCKALSNYMYTLLKAAGI
                     KSYYTLVLAGKNKSIYSDFPAVQFNHVILCVPNNGDTLWLENTDQKIPFAYLGDFTDD
                     RDVLVMAEKPFLTHTKKYTKQENIDITTATILIQENGDANINLQAKKYALCMRELYFL
                     HNYYTQKEQMMYLSSYMPLKNSQIDSFSLSLIKDRNPYIAISCSINAEKYASRTGSRL
                     FIPLNQLEKIPYTFSSDTIRNYPVFQSKDCEIIDSIKFYIPEGYTVESTPSTFKDSTD
                     FSVYQASWMINNSEIIYTRRLEINKAILDTTQYQEIRRYYNSLALRDNPMVVLKRN"
     gene            complement(5606..6589)
                     /locus_tag="PLF48_04840"
     CDS             complement(5606..6589)
                     /locus_tag="PLF48_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48684.1"
                     /translation="MKSTLLFSLFLLLFNSNSFCADVWTTQRSYRCFQIDSLYYSDNT
                     DSSLILALTQNYIIENDASLKSIDHELVSNIKQFNYNQIIRVYAKYGDCINAHKYINK
                     LLQYTNDYVYCAIKYEFTNLHSCPYWKEIRDSAEMQIQRNSALNPKLLFNLLSINDKG
                     NYNINFLAAYWDKMDSTTYLRFRDIIMLKNKNIKEEVANDLDSVISLYGYPTIKNAGY
                     DAPYIAFNIIHHSKTEILLKYQKNIDYSFKEGSIDGDNYAKYIDRVLVATNCPQRYGT
                     QRWYDEDEKMDYYFPVEDINLLNERRLALKLDPVIIPLNPSILYKEPKQKF"
     gene            complement(6728..7906)
                     /locus_tag="PLF48_04845"
     CDS             complement(6728..7906)
                     /locus_tag="PLF48_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNF48685.1"
                     /translation="MKRVFILLTCTIRISFGFAQNTSDTVSYALYKNLENSKLKHIFK
                     MMDSVGIASPIKNEVLIKHAAYFTSWNSEHQQPNYVVHVIPKDILYGNQTRTNDFRND
                     PLLKQDQPDSTDYWNSGYDRGHMAASADFKWNKKALSESYYYTNISPQNHGLNQNAWN
                     KLEMTIREWAIDYNELIVVTGPVLTANLSKIQQGSRQVSIPEYFFKIVLDYYPPKYKA
                     IAFIYPNKDVAFELDKHAVSIDSIEQLTGINFFQKLPDFIEDTIETQHHILDWDKNYF
                     KNLSASVFVPQDYGKKKINTLQAKDYIGKETCVCGKVVSTKYVENGKSNPTYINLDKK
                     FPDQVFTLIIYGNDRSNFSYPPEDFLKGKTICVKGQVGEFKGIPQIIANKEKQIEVVK
                     "
     gene            complement(8020..9033)
                     /locus_tag="PLF48_04850"
     CDS             complement(8020..9033)
                     /locus_tag="PLF48_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48686.1"
                     /translation="MRKILFLLAITSLTLLGNLSSILAQTVYTTATLTPEYKAIISKV
                     TTECNLTDVQNQKFTADYVNLVNAFNANAVKFAGNKGQMDIANNGALLNAGTKFRTYL
                     NNAQFTKLTNMIKAGQLMPPNTSTVTVPVTVTPPTTSTQTATVPTTPVAQSTTSNVTQ
                     LFREVSGFMKVSEPVSKAVIPILTEFDTKATAIKTANINNPAATKNGMDALGKEYIVK
                     LKAAGMTDEQLKQLLGAIVLQENILSGKNLSPQQQQLLNEIRTKYNLNDAQTMTVVLV
                     LVEGKLRGDAIKKTAQSNKQLAQQQTVQLMADLDNKLKTGLTPAQYTAIKSDMEKLLR
                     GGK"
     gene            complement(9178..>10101)
                     /locus_tag="PLF48_04855"
     CDS             complement(9178..>10101)
                     /locus_tag="PLF48_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNF48687.1"
                     /translation="ASGGAGGFGGGMNMDDIFSQFGDIFGDGSPFDGFFGNGRRKSDR
                     GRGQRGSNLRVKVKLDLEEIANGVQKKIKVKKHVTCDTCHGIGAKDNNSYQTCGTCNG
                     SGAVRKVTQTFLGQMATTTTCPTCEGEGKVITNKCTKCRGEGRIYGEEVVTIDIPAGV
                     SEGIQLSMSGKGNAGVRGGYAGDLLINIEENPHPHLRREDNNVLFTLVLNFADVALGT
                     HVEVPTIGGSAKIKIPKGTQSGKLFRLQGKGLPSIQGYGKGDQIVEVQVFTPQDLSHD
                     DIALLEKMKSSKAFTPDAKEKSKGFFDKLFH"
BASE COUNT         3148 a         1761 c         1808 g         3384 t
ORIGIN      
        1 aaaacgctgg gtagtgccat gagatttatt tatagtaaag atagtttttt tattgaaagt
       61 aaattactgt tccggccgca tttgcgggaa gaacaacact tcttgtatgc tggcgttatt
      121 agtcatcagc atggctaagc ggtctatgcc aaatccaatg ccggatgtag gcggcatacc
      181 gtactccaag gctctcaaga aatcataatc aatatacatg gcctcgtggt cgccgcgctc
      241 catcagtttt acttgttctt caaatcgctc acgctggtct atcgggtcgt tcaactctga
      301 ataggcattc gccatttcct tgccgtttat catcaactcg aagcgttcta ccaagccttc
      361 tttgctgcgg tgttttttcg tcagcggaga catctcaatc ggataatcag tgataaaagt
      421 aggttgtata taattatgtt cgcaacgctc gccaaaaatg gcatctatca gtttgccttt
      481 gcccatcgta ttatccactt caatattcaa ttgtttacac acatctcgca aggctgtttc
      541 atccatacca tttacatcaa atccggtgta cgtttttatc gcatcataaa tagaaatgcg
      601 cttgtaagga gctttgaaat taatcatgtt ttcgcccact tccacaaacg tagtgccgtg
      661 tacatccata gccatctttt ctaacaactg ctctgtggtt tccatcatcc agaaataatc
      721 tttgtaagcc acgtaaaatt ccagtacggt aaactccggg ttgtgcgttc tgtccatgcc
      781 ttcattccta aagttgcggc tgaattcata cacccagtca aaccccccaa ccaataatct
      841 tttcaaatat aattcgttgg cgatgcgcaa atatagggga atatccaggg cattgtggtg
      901 ggttttgaaa ggacgggcgg ccgcacctcc cggaatactt tgtaatacgg gagtatccac
      961 ttccagcgca cctttttcat tcaaaaattc acgaatggaa ttcatcatct tcgtgcgttt
     1021 taagaaagta tctttcactt gcgggttcac aattaaatct acgtaacgca tgcggtagcg
     1081 cagttcgggg tcggtaaagg catcgtgcgt atttccctcg ctgtctactt ttaccacggg
     1141 cagcggatgt agtgattttg ccagcaaaac caattcggta gcatgtacgg aaatttcacc
     1201 tactttggtt ttaaacgcat atcctttaat accgataaaa tcgcctaaat ccataagttt
     1261 ggagaagaca gtatcgtaaa gtgatttatc atcgccgggg caaatatcat ctctgcgaat
     1321 ataaatttgt attctgcctt gactatcttg tagttcggca aaagtggctt tgccagctgg
     1381 acgtgtagcc attaatcttc cggcaataca tacttcctga aaattgaggc ggtttttagt
     1441 gccgtcttct aagatttctt ctttgtagtt ttctttaatt tcggcagaat aagcatttac
     1501 cgggtacaat gctgccgggt aaggattaat acccaattct ttaatttttt caagtttttc
     1561 tctgcgtata atttcctgtt cgctcaactg ttgcatatca aaatttttga agtgcaaaaa
     1621 tacgactttt ttaatattta tttcacagtt ttggtacgat tatggctgta ttaagtatat
     1681 ttgtggaact atttaaaaat tgttattatg tatagaatat tcttgttttt aattcttttg
     1741 cttgtgctgc aagcaaatgc aaaagaaacc aaatttaaat tcggaagtgt aagtaaagaa
     1801 gaattggaaa tgaaaaatta ttcttctgac acttcagtag gtgcagtcgt actttttgat
     1861 gtcggtagta tttcgtatga agtttcagag cgtatgagtg ggcattatgt gagttacagt
     1921 tttaaacaag ttttcaacag aaaactacgt attaaaatat ttgataagaa aaatctcgat
     1981 atggctaacc aaaagatggt attgtattta ggggctcagt cagataattt tattacaaaa
     2041 aagaaagcac atactataaa ttttgagaat aataagcaag cggaaataga agttaataag
     2101 gacgaattgt ttttagacag gatttttgat aactactata tgcttaagat tccctttaag
     2161 aatgtaaagg agggctctat cattgaaatg gatattacta tagaatcgcc attgaattat
     2221 aagttaccta cctggtattt tcaaagagag attccaacac tattctcaca gttttctgtt
     2281 tcaataccgg agttttataa atttagaaaa aacatatcgg gatatcttgc aataaatcca
     2341 ccagtttctg aaattgaaga tattaccctt aattcacaag aaaatgtgca atccaataaa
     2401 acaacttgga cagcagaaaa agtgcctgca tttataagag agccttattt ggattgtgca
     2461 gataactatt tatctaagat tgaatatgaa ctttcaagtg ttcagttccc gcttcaattg
     2521 gaacaaccat taagtatgaa ctggaaggaa gtaaataaaa tgttaaatga tgcagaatat
     2581 ttgggtaaac aaataacaac atcatcaggt tttatgaaag atatgtatga tgaactcaaa
     2641 tcaaaagata atttatccaa gttagagttg atgaaagcag cttattatgc cgttaagaaa
     2701 aaaatgcgct ttaatcaagt atattcttta gcaagtttgg atggcactaa ggaggcttat
     2761 aggaaaggag aaggcaattc tgcagatatc aatttgattt tgcttaaact attaagagat
     2821 ttaaacttta atgtagcggc aattgccgtt agtacaaaaa cgaatggtaa tgtttcatat
     2881 tttccaagtt tatttgcatt taatcagctt attgtacaag taactttaga gggcaaaaaa
     2941 tatttactgg atgccacaga aaaagcctat ccaatgcatt tgctaccgga agaagatttg
     3001 aacgaccgag ggctaattat ttctgaccaa aatattgagt gggtggattt gcggacgaac
     3061 ataccttcag gaattacttt gcatgccatt gatgcaaaga ttacaaatga aggaacgatt
     3121 tcaggaaaaa ttacaataca acggaaagaa tatgcagctg caaattttaa ggaaaaacat
     3181 aatgataaag ttgatatcga ccaatatact aagaaactgg aggaagataa cctgattgag
     3241 ctccaagata aaaatatcca aaatctggac agtgttgaaa agccaataat agaaacatac
     3301 acttttgtca aacagaatca aacagaatta ggggcagcaa acatgctgta tgtaaatccg
     3361 tttattatag ataagctgag tgtgaatccg tttaagttaa atgaacgtga ctatccggtg
     3421 aattttaatc atccattcga tgaaacgtat atcatcaatc tatcattgcc ggaaggatat
     3481 gccgtaagtg aattgcctgc aaatgccagc tttagtttgc ctgacaactc aggcaagctt
     3541 acttataaaa taactgccag tgcgggcaat acaatacagc taaactgtaa attcagcatt
     3601 acaaaatcac tctttagctt tgatgaatat cctaacctta aagagtttta caatcagtat
     3661 ttaggaaagt taaacgaaat gattgtgatt aaaaaaatat aaataatgat aaataataaa
     3721 ctacttgaaa tttacgctgc catatttttc gcattttgtt ttaataatag ttttgctgat
     3781 aaaacagaac ctgtgaatgc agtaattgaa cattatgata ccaaaatgag tatagatgac
     3841 aagggatata agatacatgt ttacaagaaa atcgtactct ataatgataa tgccattgac
     3901 gaaacccgaa ttcagttgta tcattcgggt atggatgatt ctaagttgat aaaggctgaa
     3961 gtacgtgatt tatctgataa gctgattcga aaaattaaat cgtctgattt aaatgactat
     4021 ggagatttct ctaacttttc atctgatgct catgtgaagt tttatgagtt ttctgatttg
     4081 acttttccct gtacaataga gacagaatat gaatacagtg cacgttactt gttatacttc
     4141 ccgttttggg aacctgttac agattttaat gttttagtta aagatgcaag tttacaaata
     4201 agtgtagaag gcaaaacagg actgcgttat aaagaattaa atatgccaaa gactgttgag
     4261 gtaaatttgg tgggcggtgt taaaacatac acttggaagc tggttgactt taaaccattg
     4321 aaagaagaaa aatacataga tgaaatgtct cttccatttc caaaagtact gcttgcgcct
     4381 tcagaattaa attacaattc ctattccggt aaatttgaaa cttggagtga tttaggaaac
     4441 tggatattgt tactaaatgc acagcgggat aatcttgatg agcaaactac attaaaagta
     4501 aaggaaattg ctgcaaagta cccagacgat ttaagcaaaa tgaaagcact ttatgaattt
     4561 atgcaaaata cgacgagata tgtcagcatt cagtatggca taggtgggtt tcagccattt
     4621 ccggcctctt atgttgcaga aaaaggatat ggggattgta aggcattaag taattatatg
     4681 tatacgcttt taaaagcagc tggaattaaa tcgtattata cgctagtgtt agccggaaaa
     4741 aataagagta tatactcaga tttcccggca gttcagttta atcatgttat tttatgtgtt
     4801 cccaataacg gcgatacgct ttggttagaa aatacagacc aaaagattcc ctttgcatat
     4861 cttggagatt ttacggatga cagagatgtt ttggtcatgg ctgaaaagcc gtttcttaca
     4921 cacaccaaaa aatatactaa gcaagaaaat atagacataa caactgcaac gattttaatt
     4981 caggaaaacg gtgatgcaaa tataaatcta caagctaaaa aatatgcgct ctgtatgcga
     5041 gaactctatt ttctgcacaa ctattataca caaaaagaac agatgatgta tttgtcatct
     5101 tacatgcctt tgaaaaattc acaaattgat agtttttcac tttcgctaat aaaagataga
     5161 aacccatata tagctatttc atgtagcatt aatgcagaaa aatatgcatc aagaacagga
     5221 agtaggttgt ttataccgct taatcaactg gaaaaaatac cgtacacatt ttcttctgat
     5281 acgataagaa attatcccgt ttttcaatct aaggattgtg aaataatcga ctcaattaaa
     5341 ttttatatcc ctgaaggtta tacagttgaa agtacacctt caacgtttaa ggactctact
     5401 gacttttctg tgtatcaggc tagttggatg attaataata gtgaaattat ttatacccga
     5461 agacttgaga taaataaagc tatattagac actacacaat atcaggaaat acgacgttat
     5521 tataattctt tggcattaag agacaacccc atggttgtct tgaaaagaaa ttagctgtta
     5581 aaagttaact ttttacttga ttctattaga atttctgctt tggttcttta tacaggatgc
     5641 ttggattaag cggtatgata accgggtcta actttaaggc taatcttctt tcatttaata
     5701 gattaatatc ttctaccggg aagtaataat ccatcttttc gtcctcatca taccagcgtt
     5761 gtgtaccgta tcgctgtggg cagttggtcg caactaaaac tctgtctata tatttggcat
     5821 aattatcgcc atcaatagaa ccttctttaa aactatagtc tatatttttt tgatatttta
     5881 ataaaatctc tgtcttagag tgatgaatga tattaaaggc aatatagggt gcatcataac
     5941 ctgcattttt tatagttgga taaccatata agcttatcac agaatctaag tcattagcaa
     6001 cttcttcttt gatattttta ttttttaaca taattatatc tctaaaccgc aaataggtag
     6061 tgctgtccat tttgtcccaa taggctgcta aaaaatttat gttgtaattt cccttgtcat
     6121 ttattgacaa taaattgaac aatagctttg gatttaatgc actgtttctc tgtatttgca
     6181 tttcagccga atctcttatc tctttccagt aggggcatga gtgtaaattg gtaaattcat
     6241 actttatagc acaatataca tagtcgtttg tatattgcag taatttattt atatatttat
     6301 gagcattaat acagtcccca tatttagcgt aaactctaat aatctgatta taattaaact
     6361 gcttaatgtt tgatactaat tcatggtcaa tactttttaa agaggcatca ttttcaataa
     6421 tgtagttttg cgttaaagcc aatataagac tgctatccgt attatcacta taatataaag
     6481 aatctatttg gaaacaacga taacttcttt gagtagtcca aacgtcagca caaaatgaat
     6541 tactattaaa taatagtaaa aataatgaga ataacaatgt tgatttcata cgaagtgctt
     6601 tttgttgagt aaactgaatt caaataataa actcaaaatt cgcaatattg ttttattaat
     6661 aaaatagttt atatatataa attaaaaaag cactataatg tctttatgtt attttcttct
     6721 tttgggctta cttcaccacc tcaatttgtt tttccttatt ggctatgatt tgcggtatgc
     6781 ctttaaattc gccaacctgc ccttttacac agatcgtctt gcctttcaga aaatcttcgg
     6841 gcgggtacga aaaattggaa cggtcatttc cataaatgat aagtgtaaat acctgatccg
     6901 gaaatttctt gtctaagttg atgtatgtcg gattgctttt gccattttca acatactttg
     6961 tacttactac tttgccacat acacaggttt ctttgccgat ataatctttt gcttgcagcg
     7021 tgtttatttt ctttttgccg taatcttgtg gtacaaatac cgatgccgat aagtttttga
     7081 agtaattttt atcccagtct aaaatatggt gttgcgtttc aatggtatct tcaataaaat
     7141 caggcaattt ttgaaagaaa ttgatacctg tcagctgttc tatactgtcg atagaaactg
     7201 cgtgtttatc caattcaaat gccacatctt tattagggta aataaatgcg atagccttat
     7261 atttcggcgg ataataatcc aacactattt tgaaaaaata ttcgggaata gaaacttgtc
     7321 tggatccttg ttgtattttt gacaaattag ctgtcaatac aggcccggtt acaacaatca
     7381 attcattgta gtcaatcgcc cactcgcgta tcgtcatttc cagcttgttc catgcatttt
     7441 gattcagtcc atgattttgt ggtgagatat tggtgtaata ataggattca ctcaatgcct
     7501 ttttattcca tttaaaatct gcggatgctg ccatgtgccc tctgtcataa ccgctgttcc
     7561 agtaatcggt agagtcgggt tggtcttgtt ttaaaagcgg gtcgtttcta aaatcattag
     7621 tgcgtgtttg attgccatat agaatatctt taggaataac atgcacaaca tagttgggtt
     7681 gctggtgttc gctattccat gatgtaaaat atgcagcatg ttttatcagt acttcatttt
     7741 tgatggggga ggcaattcct accgaatcca tcatcttgaa aatatgtttc agcttggagt
     7801 tttctaaatt tttatacaaa gcataactga cagtgtcaga tgtattttgt gcaaagccaa
     7861 aagatattcg aattgtacag gtcagtaaaa taaaaactcg tttcataaga taaaaataga
     7921 tagaataatt ttatttttct atggattgta tggtaagctg ataaaaaaat gccggacatt
     7981 tatgcccgac attttgtagt gataattatt ttctagaact tatttaccac ctcttagcaa
     8041 tttttccata tcgcttttga tggctgtata ttgtgcaggt gttaatcctg tttttaattt
     8101 attgtcaagg tctgccataa gttgtactgt ttgttgttgt gccagctgtt tattactttg
     8161 agcagtcttt ttaatggcat ctccacgcaa ttttccttcc actaaaacta atacaacggt
     8221 catggtttgt gcatcgttca aattgtattt tgtgcggatt tcatttaaaa gttgttgctg
     8281 ctgtggagat aaatttttac cggatagaat attttcctgt aatacaattg caccaagcaa
     8341 ttgtttaagc tgttcatcag tcatccctgc agcttttaat tttacgatat attctttacc
     8401 taaagcatcc attccgtttt ttgtagcagc aggattattt atattggctg ttttgatagc
     8461 tgttgctttg gtatcgaatt ctgttaaaat tggaataact gctttagaaa caggttcgct
     8521 tactttcata aaaccggaaa cttcgcggaa taattgagta acattgctgg tcgtactttg
     8581 agcaactggc gtggtaggca cagtagcagt ctgcgtgcta gttgttggag gggtaacagt
     8641 aacgggtact gtaactgtag aggtattagg cggcattaat tgaccagctt taatcatgtt
     8701 ggttaattta gtaaattggg cattattcag atatgttctg aatttggtac ctgcattcaa
     8761 aagcgcccca ttgttggcaa tatccatctg ccctttattt ccggcaaatt tcacagcatt
     8821 agcattaaaa gcattaacca agtttacata atcagccgta aacttctgat tttgtacatc
     8881 tgtgaggtta cattctgtag ttactttgct gataatagcc ttgtactcag gggttagtgt
     8941 tgcagtagta tagactgttt gagcaagaat tgatgaaaga ttgcccaaaa gcgtaagact
     9001 tgtaatcgct aataaaaata aaatttttcg cataattgat ttctttttca caaaagtaat
     9061 caattaccat accaaaataa gctggtagtt aaagtaaatc tgtgaaaatg ctattacatg
     9121 cgaaaagctc tttagtcatg tgcttcggct taagatgcta attatatagt attctgatta
     9181 gtgaaatagt ttgtcaaaaa aacctttgct tttttcttta gcatcggggg taaatgcttt
     9241 agaagacttc attttttcta acaatgcaat atcatcatgg cttaaatctt gtggtgtaaa
     9301 tacttgtact tctactattt gatcgccttt gccatatccc tgaatactag gaagaccttt
     9361 accctgtagc ctgaacagtt taccgctttg tgtacctttt ggtattttta tttttgcact
     9421 accgccaatg gtaggcactt ctacgtgcgt acccaatgcc acatcagcaa aattaagtac
     9481 tagggtaaat agtacattat tatcctcacg tcgtaaatga gggtgtgggt tttcttcgat
     9541 atttatcagt aaatctccgg catatcctcc gcgtactccc gcatttcctt tgccactcat
     9601 agaaagttgt atgccttcac ttacaccggc cggaatatct atcgttacaa cttcctcacc
     9661 ataaattctg ccctcacctc ggcatttcgt gcatttatta gtaatgactt ttccttcgcc
     9721 ttcacaagta ggacaagttg tagtggttgc catttgccca aggaaagttt gtgttacttt
     9781 gcgtaccgcc ccactaccat tgcatgtgcc gcaagtttga taggagttat tgtctttcgc
     9841 tccaatacca tgacaggtat cgcaagtaac atgttttttt acttttattt ttttctgaac
     9901 gccattggca atttcttcca aatctaactt tactttcacg cggagattgc tgccacgttg
     9961 cccgcgacct ctgtcgcttt tgcgtcttcc atttccaaaa aaaccatcaa atggtgaacc
    10021 gtcaccgaag atatcgccaa actgagaaaa gatatcatcc atgttcattc caccaccaaa
    10081 tcctccggca cctccgctcg c
//
LOCUS       DAOQII010000070        10045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3519_length_10045_cov_5.143143, whole genome shotgun sequence.
ACCESSION   DAOQII010000070 DAOQII010000000
VERSION     DAOQII010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3519_length_10045_cov_5.143143"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLF48_04860"
     CDS             complement(<1..273)
                     /locus_tag="PLF48_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48688.1"
                     /translation="MKIFKSQFLNQLSLQAKIHASGMDEDQYEELRHSRRKFLNTSLK
                     AGAALGIASTIPLLNSCKLNSNKNTNAEKATLSQKPKVAIIGAGIAG"
     gene            435..1895
                     /gene="bchE"
                     /locus_tag="PLF48_04865"
     CDS             435..1895
                     /gene="bchE"
                     /locus_tag="PLF48_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     anaerobic oxidative cyclase"
                     /protein_id="HNF48689.1"
                     /translation="MKIFLLNPPHTAIGSRLAGEHLPPLGLLSIAGPLLDLGYQIKLL
                     DADYSNMEMENIVAEIEKFNPDILLMGHSGSTSAQPTIELIANRVKAILPAIKIIIGG
                     VFPTYHWKEILTKNKAIDIIVCGEGEEIITQLIPAIATGKIGLVDGIAYRNQHEIIKT
                     KPAKLIKNLDDYRIAWELMEGYHYTYWGKRKAVVLQFSRGCPYPCSYCGQSLFWKKWR
                     HRNPQKLADEIEILHHQYGIEVINFADENPATHQKPWIAFLKALIAKNLNLVLVGSVR
                     ADNIVRDEAYLHLYKQAGFERFLLGIENYDENVLDKIKKDGSIADDKKAIQLLRQHDI
                     LSMATYVTGFGDETWKDFYHNLKHLIQYDPDQIQLLYATPHQWTPYYDEVKDRIIIQS
                     DQRKWDYKHQILKTINLKPWQIILLVKLTEILLQIRPKALKRLFFHRDKKLRAAMRWY
                     TQIGRKVWFFEWFQFLFHDNTQADSTVMLKNFWNDF"
     gene            1929..2375
                     /locus_tag="PLF48_04870"
     CDS             1929..2375
                     /locus_tag="PLF48_04870"
                     /inference="COORDINATES: protein motif:HMM:NF022703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HNF48690.1"
                     /translation="MKKTIFLFFAVCFIIGNLSAQKTKTTTTKNKTKTTVTKSKAFEI
                     PADAYTITGNDAEDKTINTDYEKVVITGNNNKIKLTGKYTEVIVTGKDNDIEIVSVNK
                     IVVSGKGNFISWEKSTTATGKPSVQDTGGYNNVGKKSGDAMDKNDN"
     gene            2562..3905
                     /locus_tag="PLF48_04875"
     CDS             2562..3905
                     /locus_tag="PLF48_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNF48691.1"
                     /translation="MQKYIQIIKQSWRDKHFGYVLKGSLFTALSLFFVQGLRFMTGIL
                     IARFYGAEASGRLTLVVTVINTAAIFSNFGIRDAMQKLIPEYTSRYNRRTALHVFEKG
                     TKYITILSIICSIILYWVSPMLCKIWNEPDMLPYFRGSAVFVVFFVLSEYFFFSLRAA
                     FKINAANLTLVLPTVIRLLLLLLLTYYWFNINNPIFLHWITLCLLPFIFSIYPVYKNL
                     IKPAKGEVLEQQISSNTIFNLAFPMLLTYTAFLVNNSADVFMMKMFGIDTARVGIYKS
                     CTNISALATTLLVSLNTTVQPKFTQLYYSHNFDEIKKLMHKTSKLMFYISLPLFILLI
                     FFAKKIMQLYGTEFTIGAGSLAILTVGQLFNTLCGPTAQLLNATGHHKQFRNMAIIAA
                     CINILLNMILIPKYDIEGAAIAGAVSMLIWNLLSVIYIKFKFGFYSGYFPFLSSK"
     gene            3927..5099
                     /locus_tag="PLF48_04880"
     CDS             3927..5099
                     /locus_tag="PLF48_04880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF48692.1"
                     /translation="MLHRLRNIFKIEIDYSSRIFGFDVLRGFAIIIVLYTHGRTKLIN
                     FTAINQFVSIIAFWTMDMFFVLSGYLIGTMLIKTYVRDADFTGKSAYNFWIRRWFRTL
                     PNYYLLLFGTALLWLIIDGNKILFRAQFLADLFFCQSILSPPSFFLMESWSLCIEEWF
                     YLLFPLCLIAGEWILSYTKLNNIDKIKYNILACILFFYFIPLILRIHAYTLQPRLTWY
                     DVSRMTFHRIDAVSIGITFAWIDYFYKGFLKKIRIILLFVFVISFSVYIYYNYIVFGE
                     GYNRLFPLSAYIYYYLLTAISAFSLCVYMKDFKIKQVNWFVVFISYWSITSYSCYIFH
                     RSVVMYLVDAVFKTHTPIQNIPLFLLYATGSILLSIIVYKYFEHPMTELREKWKIK"
     gene            5096..6076
                     /locus_tag="PLF48_04885"
     CDS             5096..6076
                     /locus_tag="PLF48_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNF48693.1"
                     /translation="MKIGVWVPQRYDTSVKIYSDNICTILQELGVEIHYFGKNQPIPD
                     VDLIWDPTCTGARYPNRKILTTSIPWIVTLHGAANLSMPLRYTYPKLKNRFFGVFTNL
                     RRRIMWGLYKFKVKHIITVSKFAKEELVEQLNIYPDLITYIYHGYDDNLFYPQSGSKE
                     FYLHVSIYQPKKNTERIVEAYNLIKNKTNYPLLLVCPGYPDKIEDEKITLIRDKQTPK
                     SVAKLMKNAYCFIFPSLHESFGLPLVESMASGAPLITSDSSACKEICKDGGITVNPYQ
                     TEAISNAILMIEDVALHEKYTKAALERAKDFSWRKSALLHLELFKRYQYE"
     gene            6167..6808
                     /gene="udk"
                     /locus_tag="PLF48_04890"
     CDS             6167..6808
                     /gene="udk"
                     /locus_tag="PLF48_04890"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNF48694.1"
                     /translation="MTKPFIIGISGGSGSGKTTFLKRLAEDFGDDQVCLLSQDNYYRP
                     REQQLKDDNDIENFDLPESFEEELYARDVLKLIHGEDVYIKEYTYNNHLAEPKYIVYK
                     PAPILIIEGIFVFHFPQIEKMMDFKIFIDAEEHIKLIRRIQRDQLERNYPLEDVLYRY
                     QNHVFPAYQKYILPYKSHCDIVVNNNKSFENALNTVKLAVKEKLDLHEKRVAK"
     gene            complement(6983..7708)
                     /locus_tag="PLF48_04895"
     CDS             complement(6983..7708)
                     /locus_tag="PLF48_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF48695.1"
                     /translation="MYNTDFWHKHNLYGKKIILFLFCCISLHSLAQRNVLENDIKFAR
                     RPYHFGIHLAANMGDYKINHSALFAVSDSVLAITNKLGVGFEIGALFSYHINKYLELR
                     TVPSFSFTSKNLEFTFSDESKKKKDIPQIYFDFPIEIKFKSQPLRDIKVYAIGGLKYG
                     YDIGGNINDRKKTGMPHQKLNDFGVVYGVGLEIHFPLFILSPEIKAFNSVLNIHKQDQ
                     EIFAPYIKGLYNRTFTFSINFEG"
     gene            complement(7657..8373)
                     /gene="ubiE"
                     /locus_tag="PLF48_04900"
     CDS             complement(7657..8373)
                     /gene="ubiE"
                     /locus_tag="PLF48_04900"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNF48696.1"
                     /translation="MSNPVTPYNTSESKKKQVAEMFNNVAGTYDFLNHFFSLGIDKLW
                     RKKLVRMMGLQKPETILDVATGTGDLAIAEAALHPKKIVGIDISEKMLDAGKSKISKY
                     PNIEFHLGDGENIPFPDDSFDAVSVSFGVRNFENVPKGLLEMRRVLNENGKIYILEFS
                     KPKNELFKKLYYFYFCTVLPFIGKLVSKDSNAYTYLPESVKQFPDGTAFVALMEEAGY
                     KEIKCTTLTFGISTIYTAKK"
     gene            8554..9147
                     /gene="yihA"
                     /locus_tag="PLF48_04905"
     CDS             8554..9147
                     /gene="yihA"
                     /locus_tag="PLF48_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNF48697.1"
                     /translation="MIQQAKFIGAFPKCDDCPKSELVEFAFIGRSNVGKSSLINLLTQ
                     RKELAKVSQTPGKTQNINLFLIDESWYLADLPGYGYAKLSKTTREHFDKMIRFYLKNR
                     ENLACVFVLIDSSIPPQKIDLEFINWLGENAIPFSIVFTKADKGKAQEIQKNCRQFEL
                     KLLESWENLPPAFLTSSAKNQGAKGLMEFIREVVANT"
     gene            9464..9952
                     /locus_tag="PLF48_04910"
     CDS             9464..9952
                     /locus_tag="PLF48_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48698.1"
                     /translation="MKTCLQTFKVLCLLSISILTHTLKSQTIAITETLPMELLCAGQT
                     VSVSFNTTGTFNTGNSFQIQLSNAAGSFTTPTTIGTYSWAGTSAQNHTVSAAIPGATA
                     AGTGYRIRIVSTNPAITGINTFKQYIVSNRCGCNTDLIQKDFEADFGGTGSGGVCRRG
                     RR"
BASE COUNT         3262 a         1741 c         1768 g         3274 t
ORIGIN      
        1 tccggcaata ccggcaccta taatggcaac tttaggtttt tgtgaaagtg ttgctttttc
       61 tgcatttgtg tttttgttgc tattcagttt acatgaattc aacaagggaa tagtagaggc
      121 aatgcccaat gcagccccag cttttaagct tgtattcaga aattttctgc gcgaatgtcg
      181 aagctcttca tactgatctt catccatgcc actagcatga atttttgctt gcagagataa
      241 ttgattaagg aattgacttt taaatatttt cataaaattg gtttttcaaa taacaattta
      301 agcattttga aaccttttta atattaaatt ttttagcagg gaataacata ttttaattct
      361 ttacaaattt tattaacgca tcttaatatg aatacaaaaa caggttcgcc tagttttgcc
      421 attcaatttg ttctatgaaa atatttttac tcaatccacc gcacactgct atcggaagca
      481 gacttgccgg agaacatctt ccgcctttgg gcttgctgag cattgccggt ccgcttttag
      541 acttgggcta tcaaataaaa ttactcgatg ctgattacag taatatggag atggagaaca
      601 tagtagcaga aattgaaaaa ttcaatcctg atatattgct tatggggcat tccggatcta
      661 cctctgcaca gccaactatc gaattaatag caaatcgagt taaggcaatc ctgcctgcta
      721 tcaaaataat tatcggcggt gtatttccta cttatcactg gaaagaaatt ttgacaaaaa
      781 ataaagcgat agacattata gtttgtggtg aaggcgaaga aattattact caacttatcc
      841 cggctatagc aaccggtaag ataggtttgg tagatggtat tgcatataga aatcaacatg
      901 aaattataaa aacaaaaccc gccaaactta taaaaaatct agacgactat agaattgcat
      961 gggagctgat ggaaggctat cattatactt attggggaaa aagaaaagcc gttgtcttgc
     1021 aattctcgag aggctgtcca tatccatgca gttattgcgg gcaaagtttg ttttggaaga
     1081 aatggcggca tcgcaatcca caaaaactgg ccgatgaaat tgaaatattg catcatcaat
     1141 atggcattga agtcatcaat tttgcagatg aaaatcctgc aacacatcaa aagccctgga
     1201 tagcattttt gaaggcatta atagctaaaa atttgaattt agttttggtc ggttctgttc
     1261 gtgcagataa tattgtaaga gatgaagcct atttgcattt gtataagcaa gcgggatttg
     1321 agcgtttttt attaggtatt gaaaattatg atgaaaatgt tttagataaa ataaaaaaag
     1381 acggcagcat agctgatgac aaaaaagcaa ttcagttatt acgtcagcat gatatacttt
     1441 ctatggcaac ttatgtaacg ggttttggcg atgagacatg gaaagatttt tatcacaact
     1501 taaaacatct tattcaatat gatccggatc agatacaact tttatacgcc actccgcatc
     1561 aatggacacc ctattatgat gaagtaaaag acagaataat tattcagtcc gaccagcgca
     1621 aatgggatta taagcatcaa attttaaaaa caataaatct gaaaccctgg caaattattt
     1681 tattggtaaa gctcactgaa atactgttgc aaataagacc aaaagcatta aagcgattgt
     1741 tttttcaccg tgataaaaaa ctaagggcag ccatgcgctg gtacacccag attggcagaa
     1801 aagtctggtt ttttgaatgg tttcagttcc tgtttcatga taatacacaa gcagactcaa
     1861 ccgtaatgtt aaaaaacttc tggaatgatt tttgaaacgt atatttacac gtataaattc
     1921 aatttcaaat gaaaaaaaca atcttcttat tttttgccgt ttgttttata atcggcaatc
     1981 tttctgcaca aaaaacaaag acaactacaa caaaaaataa aactaaaacg actgttacaa
     2041 aaagtaaagc attcgaaatt cctgctgacg cttatacgat taccgggaat gatgcagaag
     2101 ataaaacaat caataccgat tacgaaaaag tagttattac aggcaacaat aacaagataa
     2161 aacttaccgg aaaatatacg gaagtaatcg taaccggaaa agacaatgac atcgaaattg
     2221 tatcggtaaa taaaattgtt gtaagcggaa aaggtaattt tatcagctgg gaaaaatcga
     2281 ctactgctac tggtaagcca agtgtacaag ataccggcgg ttacaataat gtcggtaaaa
     2341 aatccggtga tgcaatggat aaaaatgaca actagcgaat tcgctgttct tgtataattt
     2401 gcatatttat ttacaaatta tccttttgct aatctatagc atgaaataaa tagcatattc
     2461 tgccatttac acttaaacta ttgcttgttt attctagttt taagttgcag catgtgctgc
     2521 atttaactcc ctgaatttta ttaattttag gcactattca gttgcaaaaa tatattcaaa
     2581 taatcaagca atcctggcgc gataagcatt ttggttacgt gctgaaaggc agtttattta
     2641 ctgcgctttc actctttttt gttcaaggtt tgcgctttat gacaggtatt ttaattgccc
     2701 gcttttacgg tgctgaagct agcggcagac tgacacttgt ggtaaccgta atcaatacgg
     2761 ctgcaatttt ttcaaatttt ggcattcgtg atgctatgca aaaactaatc cctgaatata
     2821 ccagtcgata taatcggcgt accgcactcc atgtttttga aaagggcaca aaatacatta
     2881 caattttaag tattatatgt agtataatat tatattgggt atctcctatg ctttgtaaaa
     2941 tatggaacga acccgacatg ctaccctatt tcagaggatc agctgttttt gtcgtttttt
     3001 ttgttttgag tgaatatttt ttcttcagcc ttagagctgc atttaaaatc aatgctgcaa
     3061 atctcacatt agttttgcca acggtcatcc gtttgttatt gctactgcta cttacatatt
     3121 actggtttaa catcaacaat cccatctttt tgcattggat tactttgtgt ttgcttccgt
     3181 ttattttttc tatatatcca gtttataaaa atttgattaa acctgcaaaa ggagaagttt
     3241 tagaacagca gatttcatca aatactattt ttaacttggc atttcccatg ctattaacct
     3301 acacggcatt tttggtaaat aacagtgcag atgtttttat gatgaaaatg ttcggaatag
     3361 atacggctcg tgtgggtatt tataaatctt gtacgaatat ttccgcactt gccactactt
     3421 tactcgtctc gctgaatacg acagtgcaac ctaaatttac ccagctttat tacagtcata
     3481 attttgacga gatcaagaaa ttaatgcata agacatccaa gctgatgttt tatatcagtt
     3541 tgccgttatt tatcctgcta atcttttttg caaaaaaaat tatgcaactg tatggtactg
     3601 agtttaccat tggtgctggc agccttgcaa tattaactgt tggtcaactt tttaatacct
     3661 tatgtggtcc tacagcgcag ttgttgaatg caaccggaca tcataagcaa ttcagaaata
     3721 tggcaataat agcagcttgt atcaatatcc tattaaatat gatactcatt ccgaagtatg
     3781 acatagaagg tgctgctatt gccggagcgg taagtatgtt gatatggaat ctgctttctg
     3841 ttatttatat taaatttaaa ttcggttttt attccggata ttttccattt ttaagttcca
     3901 aataaatctt tttctatctt tacacgatgc tgcatcggct aagaaacatt tttaaaattg
     3961 agattgatta cagttcacga atttttggat ttgatgttct taggggcttt gccatcatta
     4021 tcgttcttta tactcatgga agaactaagc tgatcaactt tactgccatc aatcaatttg
     4081 tatcaatcat tgctttctgg acgatggata tgttttttgt tttgagtgga tatcttattg
     4141 gaactatgct gattaaaaca tatgtacgag atgctgattt tacaggaaaa agtgcttata
     4201 atttttggat aagacgctgg ttcaggactt tgccaaacta ttatttattg ctttttggta
     4261 cggcactgct ttggttaatc attgacggca ataaaatctt attcagagcc cagtttttgg
     4321 cagacctttt cttttgtcag tctatattaa gtcctcccag cttttttctg atggaatcct
     4381 ggtcgttgtg tattgaagaa tggttttatc ttttatttcc cctctgctta attgctggag
     4441 aatggattct tagttatacg aaattaaata atatagataa aataaagtac aatattttag
     4501 cttgcatctt gtttttttat tttattccct tgattctgcg tattcatgca tacactttgc
     4561 aaccccggct aacatggtat gatgtatcga gaatgacctt tcatcggatt gatgcagttt
     4621 cgattggaat tacttttgca tggattgatt atttttataa agggtttctt aaaaaaatca
     4681 gaattatatt acttttcgtt tttgtaattt ctttttctgt atatatatat tataactaca
     4741 tcgttttcgg agaaggttat aatagattat tcccactcag cgcatatatc tattattatt
     4801 tactaactgc aatttcagca ttttcattat gtgtatatat gaaagatttt aaaattaaac
     4861 aagtgaactg gtttgtcgta tttatatctt attggagtat tacttcttat tcttgttata
     4921 tattccacag aagtgtagtg atgtatttgg ttgatgccgt atttaagaca cataccccca
     4981 ttcagaatat cccgctgttc ttgctatatg caacaggctc tattcttttg tctattattg
     5041 tgtataaata cttcgaacat ccgatgacag agcttagaga aaaatggaaa ataaaatgaa
     5101 aatcggtgta tgggtacctc agcgttatga tacgagtgta aaaatttact cggataatat
     5161 ttgtaccata cttcaggaac ttggagttga aatacattat tttggtaaaa accagcctat
     5221 cccagatgta gacttaattt gggatcctac ctgtacaggt gcccgctacc cgaaccgaaa
     5281 gattctaacc acatccatac cctggattgt aactttacac ggagctgcta atttgtcaat
     5341 gcccttgcgg tatacttatc ctaaattgaa aaacagattt tttggggtct ttacaaattt
     5401 acgaagacga attatgtggg gactttacaa attcaaagta aaacatatta taacagtatc
     5461 gaaatttgcc aaagaagaat tagttgagca attgaatatt taccctgatt taattacata
     5521 tatctaccac ggctatgatg ataatttatt ttatccgcaa agcggcagta aggaatttta
     5581 tctgcatgta tccatctatc agcctaaaaa aaatacggaa cgtattgttg aagcatacaa
     5641 tttgataaaa aacaagacga actacccgct gttattggtt tgtcccggtt atccggataa
     5701 aattgaagat gaaaaaataa cgctgattag ggataaacaa acaccgaagt cggtggcaaa
     5761 attgatgaaa aatgcatatt gttttatttt tccctcctta catgaaagtt ttggcttgcc
     5821 tttagttgag tccatggcat ccggtgcacc tttaattaca tcggattcgt ctgcgtgtaa
     5881 agaaatttgc aaagacggag gaattaccgt taatccctat caaactgaag caatcagtaa
     5941 tgccatatta atgatagaag atgttgcatt gcatgaaaaa tatactaagg cagcattaga
     6001 acgagcaaaa gacttcagct ggcgaaagtc tgcattgctt cacttggagc tgtttaagcg
     6061 atatcagtat gagtagttac aagaatttca attcaaggag aaaaaaaagc agatttattt
     6121 tttctaatcc tgaatttata ccgaaattcg ccaaaacaag cggataatga ccaaaccttt
     6181 tattatcggt atttctggcg gcagtggcag cggtaaaact acatttctaa aacgcttagc
     6241 tgaagatttc ggcgacgatc aggtctgcct gctctcgcaa gataattact atcgcccgcg
     6301 agaacaacag cttaaagatg ataatgatat tgagaacttc gatttgccgg agtcatttga
     6361 agaagaatta tacgcccgcg acgtcttgaa acttatccat ggtgaggatg tatatataaa
     6421 agaatatact tacaataatc atttggcaga acccaagtat attgtttaca aacccgcacc
     6481 aattttaatc atagaaggta tttttgtttt tcattttcct cagattgaaa aaatgatgga
     6541 ctttaaaatc tttattgatg ccgaagaaca cattaagctc atacgccgca tacaacgtga
     6601 ccagttagaa cgcaattatc cattggaaga tgttttatat cgctatcaaa accatgtttt
     6661 tccggcttat caaaaatata ttctgcctta taaatcccat tgtgatattg tagtgaataa
     6721 taataagagc tttgaaaacg cacttaatac cgtaaaattg gctgttaaag aaaagctgga
     6781 tttacacgaa aaacgagttg caaagtaata accagatttc gggttaagaa aaataaggta
     6841 aaaaaatagt tgatttctca tttttcctcc aacttaacaa ggaggaggaa tagaaagata
     6901 gaaaggtggt ggttcttgat cataaaatca actattttta aattgaaacg tatatcccga
     6961 acacaggtta ataacaaggc agctatcctt caaagtttat tgaaaaagta aatgtgcggt
     7021 tatataaccc tttaatatac ggagcaaaaa tttcttggtc ttgtttgtga atattcaaaa
     7081 ctgaattaaa ggcttttatt tcaggagata aaataaaaag gggaaaatga atttctaaac
     7141 ctacgccata caccacgcca aagtcattga gtttttgatg aggcatgccg gttttttttc
     7201 tgtcattgat gttaccgcca atatcatagc catatttcag tcctccgata gcataaactt
     7261 tgatatctct taatggctga gatttgaact taatctcaat tggaaaatca aaataaattt
     7321 gcggtatatc tttctttttt ttcgattcgt ctgaaaatgt aaattcgaga tttttgctgg
     7381 taaaactaaa actcggcaca gtgcgcagct ctaaatattt attgatatga tagctgaaca
     7441 gcgccccgat ttcaaatcct actcccagtt tgttcgttat agctaatacg ctgtcactga
     7501 cagcaaaaag cgcgctatga ttaattttgt agtcgcccat attggcagcc agatgtatgc
     7561 cgaaatgata agggcggcgg gcaaatttta tatcattctc caatacatta cgctgagcaa
     7621 gcgagtgcaa gctgatacaa caaaataaaa acaggattat ttttttgccg tataaattgt
     7681 gcttatgcca aaagtcagtg ttgtacattt aatttcttta taaccagctt cttccatcag
     7741 cgcaacaaat gctgtgccat ctggaaactg ttttaccgat tcgggcaggt aagtatatgc
     7801 attcgagtct ttagatacta acttgccgat aaaaggtaaa acggtgcaaa aataaaaata
     7861 gtataatttc ttgaacaatt catttttggg tttagaaaac tccagaatgt aaattttgcc
     7921 attctcattt aacactctgc gcatttcaag aagtcctttg ggcacatttt caaaattacg
     7981 cacgccaaaa gatacactta ctgcatcaaa tgaatcatca gggaagggta tattttcgcc
     8041 atcaccaaga tgaaattcaa tattcggata cttactgatt ttagacttcc cagcatccag
     8101 catcttttcc gaaatatcga tacctacaat ctttttagga tgtaatgcag cttctgcaat
     8161 agccaagtct cctgttccgg tagctacatc caaaatagtt tccggctttt gtaatcccat
     8221 catacgcacc agtttcttgc gccatagctt atcaataccg agagaaaaaa aatgatttaa
     8281 aaaatcgtaa gtacctgcta cgttgttaaa catttcagcc acttgctttt ttttgctttc
     8341 agaagtattg tatggtgtta ccgggttaga cattagatga atggtaattg gttattagtt
     8401 aatgggtagt tagttgttag actgaacatt tatcatttat aattcaacat ttatcattta
     8461 aaattcatta ctgaattttc taaggcaaaa atacgtcttt aaacgtcaac tattttaccg
     8521 aacttagtcg ccggatatta ataaatctaa aaattgatac aacaagctaa atttattggt
     8581 gcatttccca aatgtgatga ttgtccaaaa tcagaattgg tagaatttgc ttttataggc
     8641 agaagtaatg tgggtaaatc ttctctgatt aatttattaa ctcaacgcaa agaactggct
     8701 aaagtttctc agacgccggg aaaaacccaa aatattaacc tctttttaat tgacgaaagc
     8761 tggtatttag cagatttgcc gggttatggt tatgccaaat tatcgaaaac aacccgcgag
     8821 cattttgata aaatgatcag attttacctg aaaaacaggg aaaatttagc ctgtgtattt
     8881 gtattgatag attccagtat tccgcctcaa aaaattgatt tagaatttat taactggctt
     8941 ggcgaaaatg ccattccatt ctcaatcgta tttactaaag cagataaagg caaagcccag
     9001 gaaatacaaa aaaattgcag acaatttgaa ttgaaattac tggaaagttg ggaaaattta
     9061 cccccggctt ttcttacttc ttctgctaaa aatcaggggg ctaaagggct gatggaattt
     9121 atccgagagg tagttgcaaa tacttaatct gtattctctt aatttatttt cttagtatta
     9181 atatctctat ttcatgaaat atgctgctta tgtttaatga aaaactgaaa taattttgtc
     9241 atagttaagg attttttaat atcacaaatt tacagtgtgt gtttttataa aataactgta
     9301 aataattgta tataaatgaa atacactata gctatttttt aaaagtagta aaaacaacaa
     9361 aaataatcat gtcacaatgc gctacaatta actattgata agtgtaatga atctgtataa
     9421 ttttgtattg aaacaacaga tttttaacat ttaaacccaa ctcatgaaaa cctgtttaca
     9481 aacctttaag gtattatgtc tattgtcaat ttcaatttta acacatacgt taaaatcaca
     9541 aacaatagca ataacagaaa cactcccgat ggaactgctt tgtgcaggcc aaacagtaag
     9601 tgtcagtttt aatactacag gtacttttaa taccgggaat tcttttcaaa ttcaactaag
     9661 caatgctgcc ggatctttta caacacctac aacgataggt acctattcct gggccgggac
     9721 tagcgcacaa aatcatacag taagcgccgc cattccgggt gctacggcag caggaaccgg
     9781 ttacagaatc agaatagtga gtacaaatcc ggcaattaca ggaataaata ctttcaaaca
     9841 atatattgtc agcaatcgtt gtggctgcaa taccgactta attcaaaaag atttcgaagc
     9901 tgattttggc ggaactggct ctggaggcgt ttgccgaaga ggaaggcggt gatccgctcg
     9961 acaccatccc cgcgcccgaa acgcccgcgc cggccgcagg cacggatttc cgcgaggtgg
    10021 tcacccaggc gatgctcgca ctggc
//
LOCUS       DAOQII010000071        10022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3535_length_10022_cov_5.185111, whole genome shotgun sequence.
ACCESSION   DAOQII010000071 DAOQII010000000
VERSION     DAOQII010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3535_length_10022_cov_5.185111"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..304
                     /gene="thiS"
                     /locus_tag="PLF48_04915"
     CDS             101..304
                     /gene="thiS"
                     /locus_tag="PLF48_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNF48699.1"
                     /translation="MNICINNQDYEFEENTTLYQALVFLQMQDKKGIAVAVNEAIIPQ
                     SKWDETRLNMEDKIIIIGAVAGG"
     gene            625..1410
                     /locus_tag="PLF48_04920"
     CDS             625..1410
                     /locus_tag="PLF48_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48700.1"
                     /translation="MSYIYSEIYSYIKELIFISLLININYSVADNSKNGELIDTLYYN
                     KTLNIANYDFIRTCYAKLKKIEDSFVYKNPLSLIIYDGIPINPKITDSENNSKSQIKF
                     GGYLNSLLSPLVISEVYLYKSSYFNYRPNIIINTGVHLYGYDTLYNEIEQENSTNQYL
                     ALKRLKINSIKESYFLKKPFPMIIYDNIIISDSSSTDSTEYWDIHFGNYRQVIKAFFD
                     DLDPEDISSIFIMKNYMNSDRNLIIIKSRTTRVNSNSFKKRKQ"
     gene            1444..3261
                     /gene="thiC"
                     /locus_tag="PLF48_04925"
     CDS             1444..3261
                     /gene="thiC"
                     /locus_tag="PLF48_04925"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNF48701.1"
                     /translation="MNTITTTPFPNSKKVYVKGNLNNIHVAMREISLSPTKNNGKLTA
                     NNPVTVYDTSGPYTDNNYIVDIKKGLPRLREQWITDRNDVEELYEVSSDYGKKRLHDD
                     SLHHLRFEHIAKPLRAKKGSNVTQLHYAKMGIITPEMEYIAIRENQKIEEYNKNLNGQ
                     AALLCQQHPGESFGANTPTSFITPEFVRKEVAEGRAIIPSNINHPESEPMIIGRNFLV
                     KINANIGNSAVTSSIEEEVEKAVWACRWGADTIMDLSTGKNIHETREWIIRNSPVPVG
                     TVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRVTG
                     IVSRGGSIMAKWCLAHHQESFLYTHFEEICEIMKQYDVAFSLGDGLRPGSIADANDAA
                     QFAELETLGELTKIAWKHDVQVMIEGPGHVPMHLIRENMDKQLKECHEAPFYTLGPLT
                     TDIAPGYDHITSAIGAAMIGWYGCAMLCYVTPKEHLGLPNKEDVRVGVITYKLAAHAA
                     DLAKGHPGAQYRDNALSKARFEFRWQDQFNLSLDPEKALEFHDETLPADGAKIAHFCS
                     MCGPHFCSMKITQEVRDFAEKGMQEKSEEFKEKGSEIYS"
     gene            3338..3517
                     /locus_tag="PLF48_04930"
     CDS             3338..3517
                     /locus_tag="PLF48_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48702.1"
                     /translation="MTDQEIIAKAKKALDAMKIGYEKDATSVRYHDNLSSLFNMLELN
                     KKKDRIWNLNISITF"
     gene            4104..4667
                     /locus_tag="PLF48_04935"
     CDS             4104..4667
                     /locus_tag="PLF48_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48703.1"
                     /translation="MLKLAGVSESTIEILTLQLKEVEVKSNQLLVKSGQTNTHLYYVM
                     EGGFVCRFVSEDKEIERTINFYLNELHPVMACIDSYFSQTKTSCELRAICDSKVLAIS
                     KIVLDNLIAVDPKIKMLFEQILTNALIEENDLKLKIISCKPDVLYQYLIKEFPTIIHK
                     VPSKYIAELMGISAEWLSKLKSKNRIS"
     gene            4722..6866
                     /locus_tag="PLF48_04940"
     CDS             4722..6866
                     /locus_tag="PLF48_04940"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNF48704.1"
                     /translation="MMSKSQLGYSTADKQEKFSKFYTTEFSKIPDEIIHALQNPLEWH
                     TLPDIDQMTDLEADGYLPVENGFAINKKDGSTAVAVKTEMPNVTPQMWDWWFGWHGCQ
                     DNRYKLWHPLAHISAKWEDGRNDICYIGRNSIIKEYIGANLLDAAIQFKSPMAFGFSN
                     DAVNYKEKAVYICARLGHPTLPVDYGYLIHQVRRTSNGSEMRSRFWVGGKYVAIRKNG
                     RAAEAVTSVLKMLKSLPKNFGYDLMKHCSEEMNHLAAFLPELYHEFHETKSLAISGII
                     HQKEDAHFEKTMFGFLYNQQRFGRTPDLITEPKTVEDIIHVIRYAKQVGKKVTVLSGG
                     RSFSANAMRNDCIVISMKNFTNYTINKDDMSATAEPGLGGSDLMKVLYKNDLFFPAGH
                     CKGVCLGGYLLQGGYGWNGRKLGIACKSILALDIVTADGDIVHANENENADLFWAARG
                     AGAGFFGVVVKFYLRLYQRPKYQAIIVHDFSMKHLEDVYRWAYDIGPSVPKSVEFQML
                     MSRNMMNMLGPGIEAYAPIFADTKDEFEEARQFMDNSPVKKKAIIRTPAFNPGIELLY
                     KGTMEHYPANYNYGVDNMWTHASIDELLPHIKEIANTLPPAPSHFLWLNWYPDAIDTD
                     MTYSKEDNIYLALYGCWKHQKDTIKYTSWASDKIGKMQQYSSGIQLADEGLHKRRDKF
                     LSDENFRKLQTIRAKRDPQNLFMEWHSHAFEK"
     gene            7389..8087
                     /locus_tag="PLF48_04945"
     CDS             7389..8087
                     /locus_tag="PLF48_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48705.1"
                     /translation="MKKIFFLLFAFSAIISCKHNTPANQEQQSSGTASGSSTESTSSA
                     DKIYGIKSGILVSEMDMMGMGKATVENNFDDYGNVSLTNTKASIMSMNTVTHNLRKDG
                     WMYVWTEGQKEGHKIKIDASQVSKENLDIDKLTQEMKDKMKLKEEGNSDVIGKSCKVY
                     SMTISEKGMSMAGKVYVWEKLALKSEFDAKGMKISMLPKSLDEHPNFASGFFDVPAGI
                     TFTEMQNTGQADLN"
     gene            8170..8481
                     /locus_tag="PLF48_04950"
     CDS             8170..8481
                     /locus_tag="PLF48_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNF48706.1"
                     /translation="MPVRSLSTMPDTQEEVAVLDEIEELLDTGCNLVVYNDDVNTFEW
                     VIESLMAICNHTYEQAYQCSLFIHFKGKYAVKHGSETELIPMKEALLDRHISASVENS
                     K"
     gene            8490..9191
                     /gene="aat"
                     /locus_tag="PLF48_04955"
     CDS             8490..9191
                     /gene="aat"
                     /locus_tag="PLF48_04955"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNF48707.1"
                     /translation="MPIYQLDDRLWFPDPQGAEEGIVAIGGDLEPERLLLAYSEGIFP
                     WFDDDDPIIWWSPDPRYLLIPEKFHVSRSFRRFLNKKKFTVTVDEKFAEVIENCATID
                     RKEQDGTWITEEMKVAYTNLHELGYAHSVEVWDEAHQLVGGMYGVSIGRAFFGESMFS
                     KQPNASKTALYYLSNLLSKKGLSFIDAQIHTPHMVSLGAERVTRQVFLKKLADAIQFP
                     TWKGKWSIQNELKQD"
     gene            9465..9713
                     /locus_tag="PLF48_04960"
     CDS             9465..9713
                     /locus_tag="PLF48_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNF48708.1"
                     /translation="MGKNTSILLGDYFETYVNEKISSGKYSSVSEVIRTALRLLESEE
                     NKYKILINELKVGEKSKMIKNFNRQENLKRLHTRHLPK"
     gene            9710..10006
                     /locus_tag="PLF48_04965"
     CDS             9710..10006
                     /locus_tag="PLF48_04965"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF48709.1"
                     /translation="MKYEISEKANEDIDKIWLYTFENWSLEQADRYYNLIMDEIEYIA
                     DNFESGKSLEHLRKGYRSSKVKSHIIFYKKSESNVIEIIRILHMSMDIEHRLND"
BASE COUNT         3559 a         1591 c         1914 g         2958 t
ORIGIN      
        1 gataccatcg aagggaatga gtgcaccaat acgtcctcag agttttattg caaaaaattg
       61 tacaactgct taattctttt gtactaaaaa ataaagtaag atgaacattt gtattaataa
      121 tcaagattat gaatttgaag aaaatacaac tttgtatcaa gcattagtat ttttacaaat
      181 gcaggataaa aaaggaatag ccgtagcggt gaatgaagca ataattcctc agagcaaatg
      241 ggatgaaaca agattaaaca tggaggataa aatcattatt attggcgctg tggcgggtgg
      301 gtaaaatgca attagcaaat gtggaaatgt gataatgtac cgatataccg atgtatgaaa
      361 tgtgggaatg taccgatatg ccgatgtatg agatgtggaa atgtgaaaat taggaaatgg
      421 gagaatgtac cgatataccg atttgagaaa tgtggaaatg tggagatatg aaaattagat
      481 gatttgatga tgtcaaaaat atgattatta attataatat taaattcaga aaggataatt
      541 ttcagatacc tagaaatgaa acaatatttt acatctttat tagattgttt aactcgaatt
      601 gatttagtaa ataatattta aaatatgagt tatatatata gtgaaatata ttcatacatt
      661 aaagagttga tatttatttc tcttctgatt aatattaatt actctgtagc agataatagc
      721 aaaaatggtg agttgattga cactttatat tataataaaa ctttaaacat agcaaactat
      781 gactttatac gcacttgtta tgcaaaatta aaaaaaatcg aagatagctt tgtgtataaa
      841 aacccattat cactaataat ttatgatggt attcctataa atcctaaaat tacagattct
      901 gaaaataatt ctaaaagtca aataaaattt ggcggatatc ttaattcttt attatctcct
      961 ttagtcattt cagaagttta tttatacaag tcatcttatt ttaattatag gcccaatatt
     1021 attataaaca caggagtcca tctctatggt tatgatactt tgtataatga aattgaacaa
     1081 gaaaattcaa ccaatcagta tttggcatta aaaagactaa aaattaattc tatcaaagaa
     1141 tcttatttct taaaaaaacc attcccaatg ataatatatg ataatattat cataagtgac
     1201 tcaagctcaa cagattctac agaatattgg gatattcatt ttggtaatta tagacaagta
     1261 atcaaagcat tttttgatga tttagatcca gaagatattt cttctatatt tattatgaaa
     1321 aactacatga actcagacag aaatcttatt attattaaat caaggactac aagagtaaat
     1381 tcaaactcgt ttaaaaaaag aaagcaataa aataaattcc atagcaccat atttaatatt
     1441 acaatgaata ctatcaccac cactccattc ccgaattcta aaaaggttta tgtaaagggc
     1501 aatctgaata atatccacgt tgccatgcgg gaaatttcgt tatcccccac taaaaacaat
     1561 ggcaaactga ctgccaataa tcccgttacg gtctatgata cgtcgggacc atataccgac
     1621 aacaactaca tcgttgatat aaaaaagggc ttgccgcgtt tgcgtgaaca atggataaca
     1681 gacagaaatg atgtggaaga actgtatgaa gtaagctcgg attacggaaa aaaaagactg
     1741 catgacgaca gcctccacca cctgcgtttt gagcatattg caaaaccgct gcgtgcaaaa
     1801 aaaggtagca atgtaactca actccattat gcaaaaatgg gcatcatcac gccggaaatg
     1861 gaatatattg ccattcgaga aaatcagaaa atagaagaat ataataaaaa tctgaatgga
     1921 caagcagccc ttttatgtca gcagcacccg ggcgaaagtt ttggtgccaa cacacctaca
     1981 tcttttatca ctcctgaatt tgtaagaaag gaggtagccg aaggcagggc tattatcccg
     2041 tctaacatca accatcctga aagcgaacct atgattattg gtcgcaactt tttagtaaaa
     2101 ataaatgcca atatcggcaa cagtgccgtt acttccagca tagaagaaga agtagaaaaa
     2161 gcagtttggg cttgtcgctg gggtgctgat accattatgg atttatccac cgggaaaaac
     2221 atacatgaaa cgcgcgaatg gattatccgc aattcgcctg ttccggttgg cacggtgcct
     2281 atttaccaag cgctcgaaaa agtgaatggc aaagctgaag atttgacttg ggaaattttc
     2341 agagatactt tgatagaaca agccgagcaa ggtgtggatt attttactat ccatgccggt
     2401 gtgttgttgc gttatattcc attgacggca aaacgagtta ccggtattgt gtcgcgcggt
     2461 ggcagtatca tggcaaaatg gtgtctggca catcatcagg aaagttttct atatacgcat
     2521 tttgaagaaa tctgcgagat aatgaaacag tatgatgtgg cattttcttt gggcgacggc
     2581 ttgcgcccgg gttctattgc cgatgcgaat gatgccgcgc aatttgcaga actggaaaca
     2641 ttaggcgaat tgaccaaaat agcatggaaa catgatgtgc aagtgatgat agaaggcccc
     2701 ggacatgtac cgatgcatct tatcagagaa aatatggaca aacaattaaa ggaatgccac
     2761 gaagcacctt tttatacctt aggtccattg actacagata ttgcaccggg ctacgatcat
     2821 attacatcag ccataggtgc ggcgatgata ggctggtacg gatgtgccat gttgtgttat
     2881 gtaacgccaa aagaacattt gggtttgccc aataaagaag atgtacgtgt tggtgtgata
     2941 acgtataaac tggcggcaca tgctgcagac ttagccaaag gacatccggg agcgcaatac
     3001 cgggataatg cactgagcaa agcccgcttt gagttcaggt ggcaagacca gtttaacctg
     3061 agtttagatc cggagaaagc cttggaattt catgatgaaa cacttccggc agatggtgca
     3121 aagattgcac acttttgttc gatgtgtggc ccgcattttt gcagtatgaa aattactcag
     3181 gaagtgcgtg actttgcaga aaaagggatg caggaaaaat cggaagagtt taaagaaaag
     3241 ggaagtgaga tttatagttg aatgggggat tagtttagta gtgattcaag ttttttataa
     3301 tcatctaagt tgtcatgatg aaataaaata tggttaaatg acagatcaag aaataatagc
     3361 aaaagctaag aaagcacttg atgcaatgaa aattggatat gaaaaggatg ctacaagtgt
     3421 tagataccat gataatctat cttctctctt caatatgtta gaattaaata aaaaaaaaga
     3481 caggatttgg aatctaaata tatccataac tttttaaaaa cagacttcca aacggattat
     3541 tcccaaattc aacggttaaa tgcacttttt ggggtcataa aagaagattt ttttaagttt
     3601 atttgctgtt gtaaatcaga taaaaaatgc ctcatgagta catatttgaa atactattgc
     3661 tgcttacaaa aacgaatcag ggcaaaaatt aaatttagtt aggtgttaaa aatctctacc
     3721 ttactgaaaa taatttattt tagcagggtg catataaagc caattgcaaa aaacaaccat
     3781 tgtagtttta tttgaattta tattgagaaa tagattttta gcgcatgttg aggaatgaaa
     3841 aacaaatgaa attctattgt taattcatcc cacacttcgt atcctgttat gctaaacact
     3901 ggtaaatact agaatagcga gcaacatttt tgtttttctt tttatggtag aatactattg
     3961 ccacacaatt acgtccccca aaaagtctgc aaattggtgc caattgtttt agtttgtttt
     4021 ccacgtataa catgtatttt tgccagcagt aattcagtag cataatggat ttttcttttt
     4081 catcaaaaca acaactatta gaaatgttga agctggctgg cgtatcagag agtactattg
     4141 aaatacttac actacagttg aaagaggtag aagtaaaatc taatcaattg cttgtaaaat
     4201 caggacaaac gaatactcat ttatactatg taatggaggg tggttttgta tgtcgttttg
     4261 ttagcgaaga taaagaaatt gaaagaacga ttaatttcta cctgaatgag ttacatccgg
     4321 ttatggcctg tattgacagc tatttttcac aaaccaaaac ttcctgcgaa cttcgggcaa
     4381 tatgtgattc aaaagtattg gctattagca aaatagtatt agataattta attgctgttg
     4441 atcctaaaat taaaatgctg tttgagcaaa tattaaccaa tgcactcatc gaagaaaatg
     4501 atctgaagct taaaattatt tcttgcaaac ctgatgtatt gtatcaatac cttataaaag
     4561 aattcccgac aattatccat aaagtgccat ccaaatatat tgccgagcta atgggtattt
     4621 ctgccgaatg gctaagcaaa ttaaaatcca aaaacagaat ttcttgaatt aattcaagcc
     4681 gactatttat taatgccgcc tatttttgca taaaataatg tatgatgtca aaatcacaac
     4741 taggctattc aacagcagat aagcaggaga aattctcaaa attctatact accgaatttt
     4801 ccaaaattcc tgatgaaatt atccatgcct tacaaaatcc gctggagtgg cataccttac
     4861 cagatatcga ccaaatgacc gatttagaag cggatggcta tttgcctgtt gaaaatggtt
     4921 ttgccattaa taaaaaagac ggcagcaccg cagtagccgt aaaaacagag atgccaaacg
     4981 taacgccaca aatgtgggat tggtggtttg gatggcatgg ttgtcaggat aatcgttata
     5041 aattatggca tcctttggca catatttccg caaaatggga agacggaagg aatgacattt
     5101 gctatattgg gcgaaattcc attatcaaag aatacattgg tgctaacctc ttagatgcag
     5161 ccattcaatt taaatcgcct atggcatttg gtttttcaaa tgatgctgtt aattataaag
     5221 aaaaagcagt ttatatctgt gcaagattag gtcatcctac cttacctgtt gattatggtt
     5281 atttgattca tcaagtaaga agaacaagca atggttcaga aatgcgctcg cgtttttggg
     5341 taggcggaaa atatgttgcc atcagaaaaa acggcagagc agcagaggca gttacaagtg
     5401 tattaaaaat gctaaaatca ttgcctaaga attttggata tgatttaatg aaacactgtt
     5461 cggaagaaat gaatcattta gccgcatttc tgcctgaact ttatcacgaa tttcacgaaa
     5521 caaaatcact ggcaataagt ggtattattc atcaaaaaga agatgcgcat tttgaaaaaa
     5581 caatgtttgg tttcttatac aaccaacaac gatttggcag aacacccgat ttaattacag
     5641 aacccaaaac ggtagaagat attattcatg ttatacgcta tgccaaacag gttggcaaaa
     5701 aagttacagt tttatcaggt ggcagaagtt ttagtgctaa tgccatgcgt aatgattgta
     5761 ttgtaatttc catgaaaaat tttacaaatt ataccatcaa taaagacgat atgtctgcaa
     5821 ctgctgagcc gggcttaggc ggttcagact tgatgaaagt attgtataaa aatgatttgt
     5881 tctttccggc agggcactgc aaaggggtat gtttaggcgg ctatttattg caaggcggtt
     5941 acggttggaa tggaagaaaa ttagggatag cctgtaaaag tattttagca ttggatattg
     6001 taacggcaga tggtgatata gtgcatgcaa acgaaaatga aaatgccgat ttattttggg
     6061 ctgcaagagg tgcaggagcc ggcttttttg gtgtagtagt aaaattttat ttgagattat
     6121 atcagcgacc aaaatatcag gctattattg tacatgattt ctccatgaaa catctggaag
     6181 atgtgtatag atgggcttat gatataggac cgagcgtacc aaaatcagta gagtttcaaa
     6241 tgttgatgag ccgcaacatg atgaatatgc ttgggccggg gatagaagca tatgcaccta
     6301 tctttgcaga cactaaagac gaatttgagg aagctagaca atttatggat aatagtccag
     6361 tcaagaaaaa agcgattatc agaacacccg cctttaatcc cggaattgag cttttatata
     6421 aaggcaccat ggaacattat ccggctaatt ataactatgg tgttgataat atgtggacac
     6481 atgcatctat agatgaattg ttgccacata ttaaagagat tgcaaacaca ctgccgccgg
     6541 caccatcaca ctttttatgg ctcaactggt atccggatgc tattgatacg gatatgactt
     6601 atagcaaaga agacaatatt tacttggcct tgtatggatg ttggaaacat cagaaagata
     6661 ctattaaata taccagttgg gcatctgaca agataggcaa gatgcaacaa tattcttcag
     6721 gcattcaatt agcagacgaa ggattgcata agcgaaggga taaatttttg agtgatgaaa
     6781 atttcagaaa actacaaaca attcgcgcca aacgagatcc tcaaaattta tttatggaat
     6841 ggcatagtca tgcatttgaa aagtgatagc tatatttcat aaactatctc ttagtaagac
     6901 taattttatt acaactcttt tcaagcaatt ccgttgcttt gatagcaatg taaatttgca
     6961 ttttccagta ctacaagatt aaataaaata tgtttatttt gtaaaataat tacaacaaga
     7021 aatggaaata acagatctaa aaatagacct ctcatttttg ttagtgtttt gcttagccat
     7081 gctatagcgt tgcaatcgtc tccaacaaac tatcttacag aaaataaatt ttattttacc
     7141 caggtgaata taaagccaat tgcaaatttt acccattgtg gttttatttg aatttatatt
     7201 gagaaataga tttttagcgc atgatgacgt atgaaaaacg caataaagtt ctgtcgtaga
     7261 tgcatcacac gcttttaatt atgcgatgct agaaaccaaa aataagaaaa tgaactcctt
     7321 tttattttgc caacttttat tttaattgtt ttatctttaa gattcaattc aaacttaaat
     7381 ctattgctat gaaaaaaatc ttctttttac tttttgcatt ttcggcaatt atttcttgca
     7441 aacacaatac acctgccaat caagagcaac agtcttccgg tacggcaagt ggcagttcaa
     7501 cagagagtac ctcttcggca gacaaaattt atggcatcaa atcgggcatt ctggtttctg
     7561 aaatggatat gatgggtatg ggaaaagcta ccgttgaaaa taattttgac gattacggca
     7621 atgtatcttt aaccaacaca aaagcctcga taatgtcgat gaatacggta acacataatc
     7681 tgagaaaaga cggatggatg tatgtatgga ccgaaggaca gaaagaagga cataaaataa
     7741 aaatagatgc gagtcaggta tcaaaagaaa atctggacat tgacaaactc acgcaggaaa
     7801 tgaaagataa aatgaaactg aaagaagaag gcaattcgga tgtaatcggt aaatcttgca
     7861 aagtttattc catgactata agtgaaaagg gcatgtctat ggcaggtaaa gtttatgttt
     7921 gggaaaaact ggctttaaaa tcagaatttg atgccaaagg catgaaaatc agtatgttgc
     7981 caaagagctt agacgaacat ccaaatttcg catccgggtt ttttgacgtt cctgcaggaa
     8041 ttactttcac cgaaatgcaa aacaccggac aggcagactt gaactaaaac ggtttcatat
     8101 tacatttctg tatttctttt atttgtaagg cttatcaaat ttttgtaatc ttgcatagaa
     8161 attacatgta tgcctgttcg ttcgctttcc accatgcccg atactcagga agaagtagca
     8221 gtattagatg aaattgaaga actgctcgat accggatgta atctggtagt ctataatgat
     8281 gatgtcaata catttgaatg ggtaattgaa tccttgatgg ccatttgcaa tcatacctac
     8341 gaacaagcgt atcaatgctc actttttatt catttcaaag gaaaatatgc cgtaaaacac
     8401 ggcagtgaaa cggaactgat tcctatgaaa gaagcattgc tcgaccggca tatttccgct
     8461 tcggttgaaa acagtaaata gctacgcctt tgcccattta ccaattagat gatcgtttat
     8521 ggtttcccga tccgcaagga gcagaagaag gaatagtagc cattggcggc gatcttgaac
     8581 ctgagcggtt attactggct tatagtgaag gtattttccc atggtttgat gatgatgacc
     8641 ctataatctg gtggagtccc gaccctcgat atttgcttat tcccgaaaaa tttcatgttt
     8701 cccgttcctt ccggaggttt ttaaacaaaa aaaaattcac ggttactgtt gatgaaaaat
     8761 ttgccgaagt aatagaaaac tgtgcaacta tagaccgaaa ggaacaagac ggcacatgga
     8821 taaccgaaga gatgaaagtg gcttatacca acttgcatga gttgggctat gcccactctg
     8881 tagaagtgtg ggatgaagca catcaattgg ttggcggtat gtatggtgtc agtattggga
     8941 gggcattttt tggagaatca atgtttagca agcagcccaa tgccagcaaa actgcacttt
     9001 actatctctc aaacttatta agtaaaaaag gattatcatt tatagatgca caaatacaca
     9061 cgccacacat ggtttctctg ggtgcagaaa gggtaacacg acaagtattc ctgaaaaaat
     9121 tagcagatgc aatacaattt ccgacatgga aaggaaaatg gtcaatacaa aatgaactaa
     9181 aacaagatta atttgtaagc cgttttgcag tttaaaccac atagaagcat agcattttta
     9241 tgcttaagta gttgtttggt gcaaatcagt ttagaaagtc aagtaaaaac atagtatcgc
     9301 ctttggcgat ttctgcattt tctgttctta ctactaacaa cagatatgta tatacagata
     9361 agcaaccgta tatcgaaaat acaccaagcc cactaaattt tttaacataa aatattgtga
     9421 agcaatacca aaagttggta tatttgacaa taaaatttgc acaaatggga aaaaatacat
     9481 caattttgct cggagattat tttgaaactt atgtaaatga gaaaatatct tcagggaaat
     9541 attcttcagt aagtgaagtt attagaacag cattacgttt attggaatct gaagaaaata
     9601 aatataaaat tctaataaat gaattaaaag taggtgaaaa aagtaaaatg ataaagaatt
     9661 ttaatagaca agaaaacttg aaaagacttc atacaagaca cttaccaaaa tgaaatacga
     9721 aataagtgaa aaagcaaatg aagatattga caagatttgg ttatatactt ttgaaaattg
     9781 gtctttggag caagcagaca gatattataa tttaattatg gacgaaatag aatatatagc
     9841 tgacaatttc gaaagtggta aatcgcttga acatctcaga aaaggctatc gttcatcaaa
     9901 agtaaaatct catataatat tttataaaaa atcagaaagt aatgtaattg aaataattag
     9961 aattttacat atgagcatgg atattgaaca tcgtttaaat gattaaaaac tacacataac
    10021 aa
//
LOCUS       DAOQII010000072         9975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3555_length_9975_cov_5.694153, whole genome shotgun sequence.
ACCESSION   DAOQII010000072 DAOQII010000000
VERSION     DAOQII010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_9975_cov_5.694153"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..393
                     /locus_tag="PLF48_04970"
     CDS             58..393
                     /locus_tag="PLF48_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48710.1"
                     /translation="MLPTSYQPGIVAVDMRSFLSNSVSYCLHEGLKHAGMKMRGYATN
                     NGQIIGVESRTSSPVKIPRNTDTLEHIEIKSLFPCGEGAGYAGGIMSAAIDGEKCAEA
                     VWKIVGGGS"
     gene            complement(450..1244)
                     /locus_tag="PLF48_04975"
     CDS             complement(450..1244)
                     /locus_tag="PLF48_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48711.1"
                     /translation="MNRYSYARLVFGYFLILVLYKIQATEYLFSLKGAPLKGHDVDYA
                     YLIFLATGIPQFIISHTWTCVLFDCGTLILLLACLFSDKYRHWFARFFILFFFTQRLT
                     LEIYANSHTKSISCLLFAFLPFCFKDEDIFELLVGFGRYFLLFILISAAYHKLTNGAL
                     LTSGNFVNQLINQHTDLAILQPTHISYKVAHYLILHPVLANFLFKLLWVAQLTFIAGI
                     FTKQYDRYLWIILMAFAVSTYFIMRIYNFDITVLGCSLWFYKTIKN"
     gene            complement(1259..1819)
                     /locus_tag="PLF48_04980"
     CDS             complement(1259..1819)
                     /locus_tag="PLF48_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48712.1"
                     /translation="MEIHNLYLIRVFKKSKWLFTAILLFICFQTYFFIKREYSFPFFV
                     WDMYSRTEQVTDTIVRNEIYIDDTLLNTTRLSIWQDAVLTNTFKAYWVLHENGNQDPV
                     HGLVSRRTAAFSSAWQEKISYNIENNKCDIAKFPGWIFGYIQEITHNQIQEISFEEST
                     YLYNRETTTYTKTGNKVLFEIYKKDK"
     gene            1966..2430
                     /locus_tag="PLF48_04985"
     CDS             1966..2430
                     /locus_tag="PLF48_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNF48713.1"
                     /translation="MTNDDKIVKEAKHILANYLENNNQRKTQERNVILEEIYARDDHF
                     DAEELYIQLKTKRFNVSRATVYNTLEVLVNCELVTKHQFGQNSAIYEKAYGNRNHDHL
                     ICIDCGKVFEFCDPRLQQVKTTAGDTFQQRIKTHSLTLYGNCSKQNCEHKTK"
     gene            2411..3982
                     /locus_tag="PLF48_04990"
     CDS             2411..3982
                     /locus_tag="PLF48_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNF48714.1"
                     /translation="MNIKRNNTRMMSSIRIALFMLVSYLGNALFAGEINLTPQPNSIH
                     FSEGKFTYKKGIEVKITRGDNDTKYVQNQLSSFLKSENIPQIQFSNNLLFLNLLKENT
                     IPNDGYELKIQPTQISITSSSNAGLFYGVQTLMQLINFDSTNSALSCMEIKDEPAFAY
                     RGFHLDVARHFFDVDVVKSYLDAMAKLKLNTFHWHLTDDQGWRIEIKKYPKLTETTNA
                     QRKGYYTQEQIKDIVQYAKERFITVIPEIDMPGHSSAAIVAYPYLGCYNQTIDLPQSW
                     GIKADILCPDDTTMRFVKDVLDEVCTLFPSPYIHVGGDEVNTKHWKESAEAKDIQVKQ
                     GLKNVTEIKTYFTKEIQKYLATKNKKIIGWGEIADDKIDKDIVVMSWRNNKAAIKAAK
                     NGNPVILAPRQFCYLDYMQYWDEEKKNFVYLYLPLDKAYKFNPLSKLKSHADKVLGGE
                     ACVWTAYIETENQLKNQVFPRLIAIAECLWTKPENKSYSNFTNRLDKQNNYFYKEDIM
                     HEEYDNVHIKPKKKQ"
     gene            4160..4924
                     /locus_tag="PLF48_04995"
     CDS             4160..4924
                     /locus_tag="PLF48_04995"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNF48715.1"
                     /translation="MFRHSLTLTFFFFLGLINVFAQKSHIKQGSVWYRYQLNLQLPKD
                     IKLKTEAEERTWVAPVLKQHQFYVRSVAGKEFKSKWFVGLGFAYFANGANDAINTSKL
                     IASEFRPFLEISAKQRINSRFAVNHRYKIEGRFQQKTNAAYTERLDGYNKSCRFRYQL
                     GLEYSPFQKDEKEIKLKLADEIMINAGKSVGKNIFDQNRVITAFQYNFNKKIGIELSY
                     IFWLQQLSSGDKMYNRHIMRITFINNFSISKKVKPE"
     gene            4936..5802
                     /locus_tag="PLF48_05000"
     CDS             4936..5802
                     /locus_tag="PLF48_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF48716.1"
                     /translation="MEKINLKHIAGLIVLLKVSLIAFLVWKIFSTHPNPLSSFSIDKE
                     FLLFCLAGFVAQIIDGALGMAYGVSCSTLLQSFGIPQKLVTASVHTSEVFTTGVSGLS
                     HIAMNNIDKKLFFRLVISGVISAIIAAVLISKYIDGKAVKPFISAYLLLLGIWILIKS
                     FRQFEFKNTALKYAPYLGFIGAFLDVIGGGGWGPIVTSNLIDKGNSPKEVIGTVNTAE
                     FFITFASTGVFILLVGIDSWKIILGLIAGGVVAAPIGAYIIKFIQPKTLLRLVGILIV
                     VTNTYNIYKALH"
     gene            5841..6389
                     /locus_tag="PLF48_05005"
     CDS             5841..6389
                     /locus_tag="PLF48_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNF48717.1"
                     /translation="MQAFLFQPWAWYVAGPLIALNLFLLLYFGQRFGVSTGLKTTCSM
                     LGAGNFSDFFKYNWKQETWLLVYILGSIIGGFIAFHFLYAGEAVVVSDKTANALQQLN
                     IQANQSFEPSEIFQTFTLKNIAIFIVGGFLIGFGTRWANGCTSGHAISGLSDLQLPSL
                     VAVAGFFIGGLIMTFLIIPHLF"
     gene            6394..6807
                     /locus_tag="PLF48_05010"
     CDS             6394..6807
                     /locus_tag="PLF48_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNF48718.1"
                     /translation="MKNLKFLLLGIFFGIVLAKAQVISWFRIYEMFRFESFHMYGIIG
                     SAVILGVLIVQFIKRTSLKSSDGQTIVIEPKPNTYKASIFGGILFGLGWALVGACPGP
                     IFILIGGGFFAFVVVLLSALLGTFCYGLIKDKLPH"
     gene            complement(6804..8117)
                     /locus_tag="PLF48_05015"
     CDS             complement(6804..8117)
                     /locus_tag="PLF48_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNF48719.1"
                     /translation="MRRFFPLSHPDISTLKMQLLAFADQVQQFCYLDSNNYPNYPYST
                     FGSLYAIDAIDSLSVHHDCLAQFEAFQQKNKDWLFGYLSYDLKNEIESNLHSLHFDGI
                     QHPLLHFFVPKYLVKIEKNKIEVGVYGNNDIEIKHNLAHFYNILETPKSSYNFQITNY
                     KLPQIQQRFSKLDYLETIDKIKAHIVRGDMYEMNLCQEFYAENTTIHPIATFNKLNTL
                     SKAPFTSYFKNQENYLLCASPERFLKRQKNKLISQPIKGTRKRGSTEAEDKQIKQDLF
                     FNEKERSENVMIVDLVRNDFSRIALDNTVDVEELFGIYSFEQVHQLISTITCRIAPQA
                     TFSDIIRATFPMGSMTGAPKISALHIIESLEKTKRGLFSGCVGYITPNGNFDFNVVIR
                     SILYNSAKKYLSIQAGSAITIDCNAEQEYEECLLKAQAMFKALNA"
     gene            8569..8964
                     /locus_tag="PLF48_05020"
     CDS             8569..8964
                     /locus_tag="PLF48_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48720.1"
                     /translation="MRFLLIISVLTIFLSCKKDAIKGSYNQNDLTGRWEWLSSSGGIT
                     GGTETPATRGYSQSINITADKIQFYTNDILTQDITYTIGQAVSIYDHETRTMLMQSNN
                     PICSYKIVGNKLYLYDECFDCYSSEYIRR"
     gene            9656..>9975
                     /locus_tag="PLF48_05025"
     CDS             9656..>9975
                     /locus_tag="PLF48_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48721.1"
                     /translation="MFSCKKEANGVSVTYALDSVKIENLNANDSIVNSFIVPVVEAAL
                     DSLTNPVNNIAINGDSLNIRNYASILCGSSGGFYVSDLFSIINGKPIIDSSYTPNGSC
                     GQYG"
BASE COUNT         3251 a         1742 c         1875 g         3107 t
ORIGIN      
        1 tccctaatcc ccccagtacg aaatcgctaa aaaatacagc ataaaacttc aaaagagttg
       61 ctgccaactt cttatcaacc gggaatcgtg gctgtggaca tgcgcagttt tttatcaaat
      121 tcggttagct attgtttgca cgaagggcta aagcatgcag gcatgaaaat gcggggttat
      181 gctacaaata atggtcaaat tattggagtg gaaagccgca catcttcccc cgttaagatt
      241 cctagaaata cggatacttt ggagcatatt gaaataaaat ctttatttcc atgtggcgaa
      301 ggtgcaggat atgccggagg tattatgagt gccgccatag atggcgaaaa atgcgcagaa
      361 gcggtatgga agatagtagg tggtggtagt tgataaaaaa aatcaactgt ttataaactg
      421 aaacattaac ccgaactcag gtttgtaggc taatttttaa tcgtcttata aaaccacaaa
      481 ctacatccta atacggtgat atcgaagttg tatatacgca taataaaata tgtgctgaca
      541 gcaaatgcca ttaatattat ccataaataa cgatcgtact gtttggtaaa aattcctgcg
      601 ataaaggtaa gttgtgccac ccacaataat ttgaataaaa aattggccag cacaggatgc
      661 aatatcaaat aatgtgcaac cttatagcta atatgtgtgg gttgcaggat agccagatcg
      721 gtatgttgat tgattaactg attcacaaaa tttccactcg tgagtaatgc accattcgtc
      781 agtttatgat aagccgcact gattaatata aatagcaaaa agtatctgcc gaagccgact
      841 aacagttcaa aaatatcttc atctttgaaa caaaatggta aaaatgcaaa tagcaaacag
      901 ctgatactct tcgtatgcga gttggcataa atttcgagcg tgagtcgttg tgtaaaaaaa
      961 aacaagataa aaaaacgcgc aaaccaatgc cggtatttat ctgaaaacag acaagccagt
     1021 aataatatca aagtgccgca atcaaacaac acgcacgtcc aagtatgact gatgataaac
     1081 tgtggaatgc ccgtggctaa aaatatcaaa tacgcataat ctacatcatg ccctttcaac
     1141 ggtgcacctt tcaaagaaaa taaatattcg gtggcctgta ttttatacaa cactaaaatt
     1201 agaaaataac cgaaaaccaa acgggcataa ctatatcgat tcatgtaata gagcctcgtc
     1261 atttgtcttt cttataaatt tcaaataata ccttgttccc ggttttagta tacgtggttg
     1321 tttcccgatt gtacaaataa gttgattctt caaaagaaat ttcctgaatt tgattgtgtg
     1381 taatttcctg gatatagcca aaaatccaac ccggaaactt tgctatatcg catttattat
     1441 tttcaatatt atagctgatt ttctcttgcc atgcagaaga aaaagcagcc gttctgcggc
     1501 taaccaaacc atgcaccgga tcctggtttc cattttcgtg taagacccaa taagctttaa
     1561 atgtgtttgt cagcactgca tcttgccata tcgaaagacg ggttgtattg agcaaggtat
     1621 cgtcaatata aatttcattt cgtactatgg tatcggttac ttgttctgtt ctcgagtaca
     1681 tatcccatac aaaaaatggg aatgagtatt ctcgcttgat aaaaaaataa gtctgaaaac
     1741 aaataaacag caagatggca gtaaaaagcc atttgctctt tttgaaaaca cgaatcagat
     1801 ataaattgtg aatttccatt aggttgatac cattcaaaga taatacttat ctccagctca
     1861 ttgtcttgga acacttgata tattctactt gaaatttaac atataatcgc attaataccc
     1921 tattttacaa gtactttcgc tgtaatttta cttatttttg tgccgatgac taacgatgat
     1981 aaaattgtaa aagaggccaa gcacattctg gcaaattatc tcgagaataa taaccagcgc
     2041 aaaacacagg aacgcaatgt tattttggaa gaaatttatg ccagagatga tcattttgac
     2101 gctgaagaat tatatatcca gttaaaaaca aagcgattta atgtcagcag ggctacggta
     2161 tataacactc tcgaggtttt ggtaaactgc gagttggtca cgaaacatca gtttggtcaa
     2221 aattctgcaa tttatgagaa agcttatggc aaccgcaatc acgaccatct gatttgtatt
     2281 gattgtggta aggtttttga attttgtgac ccgcgattgc aacaagtaaa aactaccgca
     2341 ggagatacct ttcaacagcg catcaaaaca cattctctga ctttatatgg taattgttca
     2401 aaacaaaatt gtgaacataa aacgaaataa tacacgtatg atgagtagca tcaggatagc
     2461 actttttatg ttggttagtt atttgggaaa tgcacttttt gcaggcgaaa ttaacctgac
     2521 accacaaccc aattccattc atttttcaga aggtaagttt acttataaaa aaggcattga
     2581 agtgaaaatt acacgtggcg acaatgatac caaatatgta caaaatcaac taagctcttt
     2641 cctaaaatcg gaaaatatac cgcagattca gttttcaaat aatcttttgt ttctcaattt
     2701 attgaaagaa aatacgattc ctaatgacgg ttatgaattg aaaattcagc cgacacaaat
     2761 ctctattaca tcttcttcca atgcaggatt gttttatggc gtacagacgc tcatgcagct
     2821 tattaatttt gattctacaa actcggcttt atcttgtatg gaaattaaag acgaacccgc
     2881 ttttgcctat cgtggttttc atctggatgt agctcgacat ttttttgatg tggatgtggt
     2941 gaaatcctat ttggatgcca tggcaaaatt gaaactcaat acatttcact ggcatttaac
     3001 ggatgaccaa ggctggcgca tcgagataaa aaaatatcct aagctgacag aaacgacaaa
     3061 tgcacaacgc aaaggatatt atactcagga gcaaattaaa gacattgtgc aatatgccaa
     3121 agaacgtttc attacggtta tcccggaaat tgatatgccc ggacacagct ctgccgctat
     3181 cgttgcatat ccatatttgg gttgctataa tcaaactata gatttgccgc aatcgtgggg
     3241 aattaaagct gatattttat gcccggatga taccactatg cgatttgtta aagatgtatt
     3301 ggatgaagtt tgcactttat ttccatctcc gtatattcat gtaggcggcg acgaggtaaa
     3361 caccaagcat tggaaagaaa gtgctgaagc aaaagatatt caagtaaaac agggcttaaa
     3421 aaatgtgacg gaaataaaga catattttac caaggaaata caaaaatatc tggcaactaa
     3481 aaacaagaaa attataggtt ggggagagat tgcagatgat aaaattgata aagacatcgt
     3541 ggtgatgagc tggcggaata acaaggctgc tataaaggct gcaaaaaacg ggaatccggt
     3601 gatactggca ccgcgtcagt tttgttatct ggattatatg caatactggg atgaagaaaa
     3661 aaagaacttc gtttatttgt acttgcctct ggataaagcc tataaattta atcctttgag
     3721 caagctgaaa tcgcatgccg ataaagtttt gggtggcgag gcttgtgtat ggacagcata
     3781 tatagaaaca gaaaatcagc tcaaaaatca agtatttcca cgcttgattg ccatagcaga
     3841 atgcctgtgg acgaagcctg aaaataaaag ctattcaaat tttacaaatc gcttggataa
     3901 gcaaaataat tacttctaca aagaagacat catgcacgaa gaatacgaca acgtgcatat
     3961 caaacctaaa aagaaacagt aggttcactc tttagattct tttagtttta atactatcta
     4021 atactacatt attcaaatag tttattttat catttgaact ttttgatgtt ataatcgata
     4081 aaagttacta aattattatc attatttcta atagtctata ttttatgtag agtttataga
     4141 tttgcaaaaa tatttttaca tgtttagaca ttctcttact cttacatttt ttttcttttt
     4201 gggtttaatt aatgtttttg ctcaaaaatc acatattaaa caaggatcgg tttggtatcg
     4261 ttatcaactg aatttgcagt tgcctaagga tataaaatta aaaactgaag cagaagaacg
     4321 aacatgggtt gctcctgtac taaaacaaca tcagttttat gtacgaagtg tggccggaaa
     4381 agaatttaaa tcgaaatggt ttgtgggttt gggattcgcc tattttgcaa atggcgcaaa
     4441 tgatgccatt aacaccagca aattaattgc atcagaattc agaccttttt tagaaataag
     4501 tgccaagcaa agaatcaatt cacggtttgc cgtgaatcat cgctataaaa ttgaaggtcg
     4561 gtttcagcaa aaaacaaatg cagcatatac ggaacgttta gacggataca ataaatcctg
     4621 tcggttcaga tatcagctgg gcttggaata ttcacctttt cagaaagatg aaaaagaaat
     4681 taaactgaaa ctggcggacg agatcatgat taatgcaggt aagagtgtag gcaaaaatat
     4741 tttcgaccaa aatcgggtaa ttactgcatt tcaatacaat ttcaacaaga aaattggtat
     4801 agaactgagc tatatttttt ggttacagca gctcagcagc ggagataaaa tgtacaacag
     4861 acatattatg agaattacat tcatcaataa ttttagcatc agtaaaaaag taaaaccgga
     4921 ataaataaat acaatatgga aaaaatcaat ttaaagcata tagccggatt aatcgtcttg
     4981 cttaaagtca gtttaatcgc gttcctggtt tggaaaattt tctccacgca tcccaaccca
     5041 ctttcgtcat tttctataga taaagaattt ctcttgtttt gcctcgcggg tttcgtggct
     5101 caaataattg atggtgcctt aggtatggct tatggtgtaa gctgttccac gctgttgcag
     5161 agcttcggca tcccgcaaaa attggtaacg gcaagcgtcc acacttcaga agtatttacc
     5221 acaggcgtat ccggcttatc gcatattgcc atgaataata tagacaagaa actttttttc
     5281 agattggtca tttcaggtgt gatatctgca attatcgctg cggtattaat ttcgaaatat
     5341 atcgacggga aagcagtaaa gccttttatc tctgcctatt tattattgct tggtatttgg
     5401 atattgatta aaagcttccg acaatttgag ttcaaaaata cagccttgaa atatgcccct
     5461 tacttagggt ttatcggggc attcttagat gtgattggcg gcggaggatg ggggccgatt
     5521 gttacgtcca atttgattga taaaggcaac tcacccaaag aagttatcgg caccgtgaac
     5581 acggcagagt tttttatcac ctttgcatct acaggtgttt ttattttatt ggtaggcatt
     5641 gattcctgga aaattatctt gggcttaatt gcaggcggtg ttgttgccgc accgattggc
     5701 gcttatatca tcaaatttat tcagcctaaa acacttctaa gattagttgg aatactcata
     5761 gtagttacca atacttataa catttacaaa gcattgcatt aacattgcaa tcttatttta
     5821 gtgttttaaa ttagttgcaa atgcaagcat ttttatttca accctgggca tggtatgtgg
     5881 caggtccgct cattgcgctc aacttgtttt tattgttgta tttcggtcaa cgattcggag
     5941 tttccaccgg gttaaaaaca acatgctcca tgcttggcgc cgggaatttt tctgattttt
     6001 ttaagtataa ctggaaacag gaaacttggt tattggttta tattttagga agtatcatcg
     6061 gtggctttat agcgtttcat tttctttacg cgggagaagc ggttgtcgtt tccgataaaa
     6121 cagcgaatgc acttcaacaa ttgaatatac aggctaatca gtcatttgag ccctcagaga
     6181 ttttccagac attcacctta aaaaatatag ccatctttat tgttggcgga tttctaatcg
     6241 gcttcggtac gcgctgggct aacggctgca cttccgggca tgccatatcc ggtttatccg
     6301 atttgcaatt gccgtcgtta gtagcagttg ccggtttttt tatcggcgga ttaatcatga
     6361 cttttttaat catcccacac ttattttaac accatgaaga atctcaaatt tttattactc
     6421 ggcatattct ttggcatcgt gttggcaaaa gcgcaagtga tatcctggtt tcgcatttat
     6481 gagatgttcc gtttcgagtc gttccacatg tatggcatca tcggcagcgc ggtaatttta
     6541 ggcgtactca tcgtacagtt tatcaagcgc acatccttaa aatcttcgga tggacaaaca
     6601 atagtaattg agccgaagcc caacacgtat aaagccagca tatttggtgg catcttgttt
     6661 ggcttgggct gggcattggt gggagcatgt ccgggaccta tttttatttt aatcggtggc
     6721 ggatttttcg ctttcgttgt cgttttgctt tcggcattat taggcacgtt ctgctatgga
     6781 ttgataaaag acaaactgcc acattaagca ttaagcgctt tgaacatcgc ctgtgctttc
     6841 aacaagcatt cttcatattc ttgttcggca ttgcaatcta ttgtaatggc gctgcccgcc
     6901 tgaatagaca ggtatttttt tgctgaatta tacaagatac ttcgtatgac cacattaaaa
     6961 tcgaaattgc cgtttggcgt gatataaccc acgcagcccg agaataaccc gcgctttgtt
     7021 ttctctaaac tttcgataat atgcagggca gaaatttttg gcgcacccgt catgctgccc
     7081 atcgggaatg tagcacgaat aatgtctgag aatgtagcct gcggggcaat gcgacaagta
     7141 atcgttgaaa taagctgatg aacttgttca aaagaataaa tgccgaacag ctcttctaca
     7201 tcaactgtat tatccagagc aatacgcgaa aaatcattgc gcaccaaatc gactatcatc
     7261 acattctcgc tgcgttcttt ctcattgaaa aataaatctt gttttatttg tttgtcttct
     7321 gcctcagtac tacctcgctt gcgggtgcct tttatgggct gagaaatcag tttgtttttt
     7381 tgtcgtttca aaaatctctc cggtgatgca cagagcaagt aattttcctg atttttaaaa
     7441 taagaagtaa acggagcttt agaaagagtg ttgagtttat tgaatgtggc tatgggatgt
     7501 atagtagtat tttcagcata aaattcctgg cacaagttca tttcatacat atcgccgcgc
     7561 acgatatgtg ctttaatttt atcgatggtt tcaagataat ctagtttaga aaaacgctgt
     7621 tgaatttggg gtaatttgta atttgtaatt tgaaagttat aagaagattt tggtgtttct
     7681 aatatgttat aaaaatgcgc taagttgtgt ttaatctcga tatcgttatt gccatacaca
     7741 ccaacttcga tcttattctt ttctatttta accagatatt tcggcacgaa aaaatgcaac
     7801 agaggatgct gaatgccatc aaaatggaga gaatgtaaat tggattcaat ttcgtttttc
     7861 aggtcataag ataaatagcc aaaaagccaa tccttgtttt tttgctgaaa ggcttcaaat
     7921 tgggcaagac aatcatgatg tacgcttaag ctgtctatcg catctatggc atataaagag
     7981 ccaaaagtag aatacggata gttgggataa ttattgctgt ctaaatagca aaactgctgc
     8041 acctgatcag caaaggctaa gagttgcatt ttcagtgtcg aaatatcggg atgagacagt
     8101 ggaaaaaatc ttctcatgac ttaaagccga tgtaagatac aagactttat caaaaataca
     8161 gttaggtaag gtacagatat gaaagttgtg acaatagtct aagcttttac atgcttgctg
     8221 aattcaagta ttaaactatg ttggagtagg tcaataccca aacgagccca aaatgcaagg
     8281 tgccgccact acgtggcttg ttattatggt aaaagtgttt attaaaaagg tattgccgct
     8341 acgtggcaga accaattcaa tcagttcccc caaaaaaaag gaaacagtta taagcatgca
     8401 aaccgtaaac tctttgcatt ttgtcaggtg tccacacttg atataattat atattatatc
     8461 attattccaa gtgtattaga aaaaatattt ttttgactaa aaaacttgac aaatttgtct
     8521 aatcttatta tctttaaaaa aaataatttt caacctaaat taaatttcat gagattttta
     8581 cttattattt ccgtgctaac tatttttctt tcttgtaaga aggatgcaat taagggttca
     8641 tataatcaaa atgatttaac aggaagatgg gaatggttaa gttcaagtgg aggtattaca
     8701 ggtggcactg aaactcctgc cacaagagga tattcacaat caattaatat tacggcagat
     8761 aaaatacaat tttacaccaa cgatatccta acgcaggata taacatatac aattggacaa
     8821 gctgtttcaa tttatgacca tgaaactcga accatgttaa tgcagtctaa taacccaatt
     8881 tgttcatata agatagtagg gaataaattg tatctatacg atgaatgttt tgattgttat
     8941 tcttctgaat atattagaag atagcctact ttgtttcaga cattaagtat ccctagttat
     9001 tttactgcac aattacatca tttatatctc tacagactaa gtgcattcat cggtttcacc
     9061 tgaaaccaat caaatttggc aaatcttatc aaacaatact tattgacaaa aaaattacct
     9121 attaatatac ataaatcatc atcctgtgat tatataagaa catgagttca aaataaaaat
     9181 aatgatatgt cccttgctca ctacgttcgc ctttgttggt ttgtaaccaa caaactacga
     9241 accgtaaaga gttataagaa taccccaaac tggcgcaaga atgaatcgca ggggcgtgcg
     9301 ttagcttctt gtgccaaaat gaaaggtgtc gccactacgt ggcataataa acaacaagtc
     9361 cccaaaaagg aaactgtttt aagcaagcac accataaact caccacttgg aggggctggg
     9421 ggtgggtaat acccaaaatc atgtgattat gtacccaaac aagatacgag atcttattaa
     9481 attataagtt gtaagtagtt taatttcagt aatttacaag taaagttaaa ttcggattgt
     9541 tctacaaact gaaggatttg ttttaatttt aataatgtat gtttgctaaa tttgttgaaa
     9601 gattaatcaa atgaagcaaa tactattctt atcaatactc atcgcaactt gttttatgtt
     9661 tagttgcaaa aaagaagcaa atggcgttag cgttacatac gcattagatt cagtaaaaat
     9721 agaaaatcta aatgcgaatg attcgattgt aaattccttt atagtaccgg ttgttgaggc
     9781 tgcattggat agtttgacaa atcctgttaa taatatagct atcaatggtg atagcttgaa
     9841 tattagaaac tatgcttcaa ttttatgtgg aagtagtggc ggtttttatg tttctgattt
     9901 gttttctatt ataaatggaa aaccaatcat agattcatcc tatacaccaa atggttcttg
     9961 cggacaatac gggaa
//
LOCUS       DAOQII010000073         9948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3567_length_9948_cov_5.192864, whole genome shotgun sequence.
ACCESSION   DAOQII010000073 DAOQII010000000
VERSION     DAOQII010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3567_length_9948_cov_5.192864"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="sufB"
                     /locus_tag="PLF48_05030"
     CDS             <1..983
                     /gene="sufB"
                     /locus_tag="PLF48_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNF48722.1"
                     /translation="EAIREHGDLVKQYIGTVVPQKDNFYAALNSAVFSDGSFCYIPKG
                     VRCPMELSTYFRINAAGSGQFERTLLIADEGAYVSYLEGCTAPQRDENQLHAAVVELI
                     ALKDAEIKYSTVQNWYPGDKNGKGGIYNFVTKRGICKGDNSKISWTQVETGSAITWKY
                     PSCILAGDNSIGEFYSVAVTNNFQQADTGTKMIHIGKNTKSRIVSKGISAGKSQNSYR
                     GLVKVHHKAENARNFSQCDSLLMGDQCGAHTFPYLEISNNSSKVEHEATTSKIGEDML
                     FYFEQRGIDEETAVAMIVNGFCKEVMDHLPMEFAVEARKLLEISLEGSVG"
     gene            1019..1624
                     /locus_tag="PLF48_05035"
     CDS             1019..1624
                     /locus_tag="PLF48_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48723.1"
                     /translation="MKEFKSDIESCFYYPILIGLAFIFLFYINLIPLTILIISLFFCL
                     GISVLILFGDNSVYIEKESIKVIKSNLLFNKTELIDKTELKSISFIPNLFETTNLLQI
                     QNNMHKWEILNNILSFLLFPFIKIIQFTMPNFIQIIKIEDKNGIKNSFLCCGIQEDHY
                     TNSDKNTFENLIKFIHKYEYPIIMTNKIRDIIFILNESPKT"
     gene            1826..2038
                     /locus_tag="PLF48_05040"
     CDS             1826..2038
                     /locus_tag="PLF48_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48724.1"
                     /translation="MSTSTIQPNLSNLQLELLKLYATGVEEEDLLEIKLMLSRYFLKK
                     AVNEASKVANEKNYKQTDFDNWLNEK"
     gene            2038..2445
                     /locus_tag="PLF48_05045"
     CDS             2038..2445
                     /locus_tag="PLF48_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNF48725.1"
                     /translation="MLHVVLDTNIIVSALANTSTYHTIIEAYFNNAFALCISNEIVLE
                     YEEVLKSKYNLQTSESFINAITINPTTKFSHIHYKWQLIYKDLDDNKFVDCAIQNNAI
                     LVTNDKHFNTVKQINFPSLTIININEFIELLKK"
     gene            2484..3233
                     /gene="sufC"
                     /locus_tag="PLF48_05050"
     CDS             2484..3233
                     /gene="sufC"
                     /locus_tag="PLF48_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNF48726.1"
                     /translation="MLKINNLHVSIDNKEILKGFSLEVNKGEIHAIMGPNGTGKSTLG
                     NVLAGKEGYEITQGTVEFDGQNLLELEAHERAQAGLFLAFQYPVEIPGVSNANFLKAA
                     INEIRKSKGEEPMPPNEFLKMMREKMELVEMKKDFISRSVNQGFSGGEKKRNEIFQMA
                     MLNPKLAILDETDSGLDIDALRIVANGVNKLHTKENAFIVITHYQRLLDYIVPDFVHV
                     MYDGKIVKTGDKSLALELEEKGYEEFENVVV"
     gene            3337..4554
                     /gene="sufD"
                     /locus_tag="PLF48_05055"
     CDS             3337..4554
                     /gene="sufD"
                     /locus_tag="PLF48_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNF48727.1"
                     /translation="MKEELENILPTVKDKNQHASLQNAIEIGIPTMKQEEWKYTYYNK
                     ILQQGFTPAIQIERKEIAKEFVEAHSISKNKLVFVDGIFHDELSALIYHPKINFWNQN
                     NSAESNSSNFLIELNNAFSNNGIFLEIGDQVIADELIEIFYINTNTSNVINVKNKLKI
                     GKNVQAKFAEYYLNYETQATYINTVTEVDIAEQALLEWYKIQDAGDTSNITDNLIVTQ
                     AKNSVCNCTTITFTGKIIRNVSQFDVNGERAETHMNGLYLLLNEEHVDNRTLVKHNVA
                     NCESYELYKGILGGKSTGVFNGKIVVAQAAQKTNAFQSNRNILLSNDANIYTKPQLEI
                     FADDVKCSHGATSSQIEDSELFYLQARGIGKEAAKSLLVFAFGEEVIDKINYTPLKEK
                     LEKRIAELLKIEL"
     gene            4642..5871
                     /locus_tag="PLF48_05060"
     CDS             4642..5871
                     /locus_tag="PLF48_05060"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNF48728.1"
                     /translation="MKQTMLDINKIRNDFPILSEKIRGKQLVYLDNGASTQKPKSVID
                     AESFYYEHQYANIHRGVHYLSQIGTDLYEQVRRQIQHFINAKHEHEIIYTKGTTDSIN
                     LVAYTFGRKFINEGDEIVVTEMEHHSNIVPWQMLCEEKKCTLKYIPLQQDGTILLEDA
                     KKIITSKTKLVAVTYVSNALGTINPIEELISIAHQNGAKILIDAAQAIQHFSIDVQAL
                     DCDFLVFSGHKIYGPTGTGILYGKEEILNAMPPFEGGGDMIKSVSFSGTVFNDLPFKF
                     EAGTPNIAGCIALGSAIKYVQDVGLDNIKNYENELLQYATEKLTAIDGLKIYGTAEEK
                     SSVISFLLDGIHPYDVGVILDNLGIAIRTGHHCAQPVMDYYKIPGTCRASFAFYNTKE
                     EIDLLYDGILKANKMLN"
     gene            5881..6354
                     /locus_tag="PLF48_05065"
     CDS             5881..6354
                     /locus_tag="PLF48_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNF48729.1"
                     /translation="MNKNELLIQKFYTAFQQKDWKTMQACYHPEVEFNDAVFKNLKGK
                     KAGAMWHMLLSGSKDLDLIFDGIEANETNGNAHWVATYTFTLTGKKVVNDIHAVFEFR
                     DGLIIKHTDSFDFKKWSKQAFGFTGILLGGSTFFQNYLTKKTDKRLDVFMKNKNY"
     gene            6394..7218
                     /locus_tag="PLF48_05070"
     CDS             6394..7218
                     /locus_tag="PLF48_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNF48730.1"
                     /translation="MMNYIKEIIDYKIHIYGKHELEVASVFLIAVTIIIFYLIIKITK
                     KSIYNTKHFDSAKKYTIYTLTKYILVIIMMIIILEILGLNLSVLLIGSSAILVGVGLG
                     IQNLFNDYISGIIILLDSTVKVGDIIETNGIICTIQQINLRTTQAITREDKYIILPNS
                     LLTKSEIVNWTHGERDSRFEISIGVSYNADIKFAMQLMTDAATEHDKVNKTPIPIARL
                     ADFLDSSIQLTLLFWLNDTFRAENYKSQIRMSIYEKFKQNNIEIPFPQVVIHRSDK"
     gene            7223..7645
                     /locus_tag="PLF48_05075"
     CDS             7223..7645
                     /locus_tag="PLF48_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNF48731.1"
                     /translation="MMKSINEIQDEIIDDFSMYEDWNDKYEFLIDYGKSLQPLDEKYK
                     TEENRIHGCQSRVWLHAEKKEDKLFFYADSDAIITKGIVGLLVKVLSGHSPKDIAEAN
                     LYFIEKIGLKEHLSPTRANGLVNMIKNMKLEAVKNINH"
     assembly_gap    7782..7881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7903..8310
                     /locus_tag="PLF48_05080"
     CDS             7903..8310
                     /locus_tag="PLF48_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF48732.1"
                     /translation="MTKKQITQLSYEIIGYAIKVHKALGPGLLESVYATCLKYELEKN
                     KHQVVKELSVPVYYDNIIMNTELRLDLLVDDMIIVELKAIENILPVHEAQLLTYMKLL
                     KKPQGLLINFYTDNVTKSMKPFVNEYFSELEDE"
     gene            8341..8661
                     /locus_tag="PLF48_05085"
     CDS             8341..8661
                     /locus_tag="PLF48_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNF48733.1"
                     /translation="MQDKSFMEIKDEAISQIQTIYDPEIPVNIYELGLIYEVNVDMEN
                     HVHVLMTLTAPNCPAAESLPVEVEQKITAIEGVKNCKVEITFDPPWDQEMMSEIAKLE
                     LGFL"
     gene            complement(8678..>9948)
                     /gene="dnaB"
                     /locus_tag="PLF48_05090"
     CDS             complement(8678..>9948)
                     /gene="dnaB"
                     /locus_tag="PLF48_05090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNF48734.1"
                     /translation="IDLLTVTEELRKDGKLEEVGGAFYLSELTNKIASSANVEYHARI
                     VIQKFIQRELIRISNDIIRDAYEDTTDVFDLLDTAEKRIYEITDKNLRNSTQGIGQLV
                     SKSILQIDDLLNREDGLLADSVPSGYIELDKMTSGWKATDLIIIAARPSMGKTAFVLN
                     LARNGAVDHNMPVAIFSLEMGAVQLAKRFIALECELDAQKIANGRLSDDEYALLRDKV
                     EHLSAAPIYINDQPAINIYELRAQCRRLQNAHGIKMVIIDYLQLMSGGGDKGMNREQE
                     ISSISRSLKGLAKELNIPVIALSQLNRSVETRGGEKKPQLSDLRESGSIEQDADMVMF
                     LYRPEYYNMNEGSSGEPLKGVAEVIIAKHRNGPTGVVPLRFNKNFGRFYDAGGLFDEL
                     EEMTSYKTAPSKGNFMKDDEDKDSGAFTIY"
BASE COUNT         3579 a         1654 c         1772 g         2843 t
ORIGIN      
        1 gtgaagccat ccgagaacat ggcgatttag taaaacaata tatcgggacg gtagtgccgc
       61 aaaaagataa tttttatgct gcgctgaaca gtgcagtgtt ttctgacggt tctttttgtt
      121 atattccaaa aggtgtgcgt tgccctatgg aactgagtac gtatttccgt atcaatgctg
      181 caggttccgg gcaatttgaa cgcacgttgt taattgctga tgaaggtgct tatgtttctt
      241 atttggaagg atgcaccgct ccacagcgcg acgaaaacca actacatgct gctgtggtag
      301 agttgattgc attgaaagat gcggaaataa aatacagtac agtacaaaac tggtatccgg
      361 gcgataaaaa cggaaaaggc ggtatttata attttgttac caaacggggt atatgtaaag
      421 gcgataattc aaaaatttca tggacgcaag tagaaacagg ttccgcaata acatggaaat
      481 atccgtcgtg tattttagca ggagataata gcattggtga gttttattcg gtggcagtta
      541 ccaataattt tcagcaagcc gataccggaa cgaagatgat tcacattggc aaaaacacaa
      601 aaagccgcat tgtatcgaaa ggtatttctg ccggaaaatc gcaaaacagt tatagaggac
      661 tggtgaaagt gcatcacaaa gcggaaaatg cccgtaattt ttcacaatgt gattccttat
      721 tgatgggcga ccagtgcggt gcgcatacat ttccgtattt agaaataagc aacaacagtt
      781 ctaaggtaga acacgaagca accacttcaa aaattggcga agatatgttg ttttatttcg
      841 agcaaagagg gatagatgaa gagactgccg tagctatgat tgtaaatgga ttttgcaaag
      901 aggtaatgga tcacttaccg atggaatttg ccgtagaagc cagaaaatta ttggagataa
      961 gtttggaagg aagtgttggg taaataattt gaagatttga taattagaaa atttgaaaat
     1021 gaaagagttt aaaagtgata ttgaatcttg tttttattat ccaattttaa taggtttagc
     1081 ttttatattc ttgttctata ttaacctaat accattaacg atactcatta tttcattatt
     1141 tttttgttta ggaatttcag ttctaattct ttttggtgat aattcagtat atattgaaaa
     1201 agaatcaatc aaggttatta aatctaattt attatttaat aaaacagagc ttattgataa
     1261 aacagaactt aaatcaatct catttatccc aaatttattc gagacaacca acctattaca
     1321 aattcaaaat aacatgcata aatgggaaat tctaaataac attctatcct ttctcttatt
     1381 tccttttatt aaaattattc aattcacaat gcccaatttt attcagataa ttaagattga
     1441 agataaaaat ggaattaaaa attcattctt atgctgtgga atacaagagg accattatac
     1501 aaattcagat aaaaatactt ttgaaaactt aattaagttt atacataagt atgagtatcc
     1561 aattataatg acaaataaaa ttagagacat aatattcatc ctaaatgaat caccaaaaac
     1621 ataacaatca aattaaacat aacaatcaca gttcagacaa ataaaatgaa accacaaagt
     1681 acactaaaaa atgacattag gcacaatgca aaatctctgt gaacattgcg aatcaacatt
     1741 gcgaacattg cggtaaaaaa taaattagaa atctttgcat ctttgcgtga aacaaaaaaa
     1801 attagtaact ttaaaagtaa taaagatgag cacctcaact attcaaccaa atttaagcaa
     1861 cttgcaatta gagttgttaa agttatacgc caccggcgta gaggaagaag atttgctgga
     1921 aatcaaactt atgctctcca gatacttttt aaaaaaagcg gttaacgaag cttcaaaagt
     1981 tgctaatgaa aagaattata agcaaactga tttcgataac tggctgaatg aaaagtaatg
     2041 ctacacgttg ttttagatac caatattatt gtaagtgcat tggcaaatac ttctacctat
     2101 catacgataa ttgaagctta ttttaataac gcgtttgcct tgtgcatttc aaatgaaata
     2161 gtcttagaat atgaagaagt attaaaaagc aaatacaatc ttcaaacatc agaatcattt
     2221 attaatgcta tcactattaa tccgacaact aaattttctc atattcatta taaatggcaa
     2281 ttaatttata aagatttaga tgacaacaaa tttgtggatt gcgccataca aaacaatgct
     2341 attttagtta cgaatgacaa acatttcaac actgtaaaac aaatcaactt tccttcatta
     2401 acaataataa atatcaacga atttatagaa ttactaaaaa aataaaatca ttccgcttca
     2461 ttgctggatt aaaataaaca accatgctaa aaataaacaa cttacacgta tctatcgaca
     2521 acaaagaaat cttgaaaggt ttttctttgg aagtcaataa aggagaaata cacgccatca
     2581 tgggacccaa tggtaccgga aaatctacct taggcaatgt attagccgga aaagaagggt
     2641 atgaaatcac acaaggcact gtcgaatttg acgggcagaa cttattggaa ctcgaagcac
     2701 atgaaagagc gcaagcgggt ctgtttcttg catttcagta tccggtagaa attccgggtg
     2761 tgagcaatgc taatttcttg aaagctgcca tcaacgaaat acgaaaatca aaaggtgaag
     2821 aaccaatgcc accgaatgaa tttctaaaaa tgatgcgcga gaaaatggaa ttagtagaga
     2881 tgaaaaaaga ttttatttcc cgatctgtta atcaaggatt ttctggtggc gagaaaaaac
     2941 ggaatgagat tttccaaatg gccatgctca atcccaaact tgccatcctg gatgaaactg
     3001 attccggttt ggatattgac gcgttgcgca tcgtagccaa tggcgtaaat aaattgcata
     3061 ccaaagaaaa tgcgtttatc gtcatcactc actaccaacg tttgttggat tatattgtcc
     3121 cagattttgt gcatgtcatg tacgatggaa aaattgtaaa aaccggggat aaatccttgg
     3181 ctttagaatt agaagagaaa ggatatgaag agtttgaaaa tgtagtagtg taatgagcac
     3241 cgtcatttcg agtgtagcga gaaatctcga attgaatggc tctaaataaa ttgaacagat
     3301 ttctactatc gtcgaaatga cgtaaaacaa aaaacaatga aagaagaact agaaaatata
     3361 ctgccaactg tcaaagacaa gaatcagcat gcatctttgc aaaatgctat tgaaataggc
     3421 attccgacaa tgaagcaaga agaatggaaa tacacgtatt acaataaaat tttacagcaa
     3481 ggatttactc cggctataca aattgaaaga aaagaaattg caaaagaatt tgttgaagca
     3541 cactccataa gtaaaaataa gctggttttt gtagatggta tttttcatga tgaattatct
     3601 gcattaatct atcacccgaa aattaatttc tggaatcaaa ataacagcgc tgaaagcaac
     3661 agcagcaact ttttgataga actgaataat gcattttcaa acaatggtat ttttttagaa
     3721 ataggagatc aggtaattgc agatgagctc attgaaattt tctatatcaa tacgaacaca
     3781 agcaatgtca tcaatgtaaa aaacaagcta aaaatcggca aaaatgtaca agctaaattt
     3841 gccgaatatt atctgaacta tgaaacacaa gcaacatata ttaatacagt tacagaagtg
     3901 gacattgcag aacaagcttt attggaatgg tataaaatac aagatgcggg agacacttcc
     3961 aatattaccg acaatctcat tgttacacaa gccaagaatt ctgtttgcaa ttgcacgacc
     4021 attacattta caggtaaaat tattcgcaat gtttcgcagt tcgatgtcaa tggagagcga
     4081 gcggaaacac acatgaacgg gctgtattta ttgctgaatg aagaacatgt ggacaacagg
     4141 actttagtca aacacaacgt agccaactgc gaaagttatg agttgtataa aggtattttg
     4201 ggaggaaaat ctacgggggt gtttaatgga aagattgtag tggcgcaagc tgcacaaaaa
     4261 acgaatgcat ttcagagcaa ccggaatatt ttattgagca atgatgctaa tatttatacg
     4321 aagccacaat tggaaatttt tgctgatgat gtaaaatgct cacatggagc cacatcttca
     4381 caaattgaag acagcgaatt attctatttg caggctcgcg gtattggcaa agaagcggca
     4441 aaaagtttat tagtatttgc ctttggcgaa gaagtaattg ataagataaa ttacacaccc
     4501 ttaaaagaaa aattagaaaa acgaatagca gaattattga agattgaatt ataatgcaca
     4561 tcatttcgag cgcagcgaga aatctcgaaa tggatagcac taaataaatt gaacagattt
     4621 ctcctatcgt cgaaatgacg aataaaacaa acaatgttag atataaataa aatacgcaac
     4681 gacttcccca tcttaagcga aaaaattcgt gggaaacaac tcgtttattt agacaatggc
     4741 gcatctacac agaaacctaa aagtgtaatt gatgcagaaa gtttttacta cgaacatcaa
     4801 tatgccaata tccatcgagg tgtgcattat ttatcgcaaa ttggcactga tttatatgaa
     4861 caagtgcgca ggcaaataca acattttatc aatgccaaac acgagcacga aattatttat
     4921 accaaaggaa ctacagacag tatcaattta gtggcatata cctttggaag gaaattcatt
     4981 aacgaaggcg acgagattgt agttacggaa atggagcatc actctaatat tgttccgtgg
     5041 cagatgttgt gtgaagaaaa aaaatgtact ttaaaatata ttccgcttca gcaagacggc
     5101 acgattttat tggaagatgc gaaaaaaatc atcacatcca aaacaaaatt agtagccgtc
     5161 acttatgttt ctaatgcatt aggcaccatc aatcccattg aagaattgat atcaattgcc
     5221 catcaaaacg gagcaaaaat cttgatagat gcagcccagg caatacaaca tttcagcata
     5281 gacgtgcaag ccttagattg tgatttttta gtattctccg gacataaaat atacggccct
     5341 acaggtactg gaatattgta tggcaaagaa gaaattctaa atgctatgcc tccttttgaa
     5401 ggtggcggcg atatgataaa atctgtttca ttttcaggaa cggtgttcaa tgatttgcca
     5461 tttaagtttg aagcaggcac acccaatatt gccggttgta tcgcattagg tagtgctatc
     5521 aaatatgtgc aagatgttgg attagacaat atcaaaaatt atgaaaatga attgttgcaa
     5581 tatgctactg aaaaattaac tgcaattgac ggactgaaga tttatggaac tgcagaagag
     5641 aaatcttcgg taatatcatt tttgttagat ggaatacatc cttatgacgt gggtgttatt
     5701 ttagataatt tgggcattgc cattcgcacc ggacatcatt gtgcgcaacc agtgatggat
     5761 tattataaaa tcccaggtac atgcagagca tcatttgcat tttacaatac gaaagaagaa
     5821 attgatctac tttatgatgg aattttgaaa gcaaataaaa tgttgaacta acaattgagt
     5881 atgaataaaa atgaactatt aattcaaaaa ttttatacag cttttcaaca aaaagactgg
     5941 aaaactatgc aagcctgtta tcatccagaa gtggaattta atgatgctgt ttttaaaaat
     6001 ttaaaaggta aaaaagcagg tgctatgtgg cacatgttgt tatcaggcag caaagattta
     6061 gatttaattt ttgatggaat tgaagctaac gaaactaatg gaaatgcaca ctgggtggca
     6121 acgtatactt ttactctaac tggaaagaaa gttgtgaatg atatacatgc tgtttttgaa
     6181 tttcgtgatg ggctaatcat caaacatacg gatagttttg atttcaaaaa atggagcaaa
     6241 caagcatttg ggtttacagg aattttatta ggaggctcca ctttcttcca aaattatctg
     6301 actaaaaaga ctgacaaacg acttgatgtt tttatgaaaa acaaaaatta ttgattttag
     6361 aatgccggag aacggcgtat taaataataa aagatgatga actatataaa agaaataatt
     6421 gactacaaaa ttcacattta tggcaaacat gagctggaag ttgcatctgt ttttttaatt
     6481 gcagtcacta tcatcatttt ttatttaatt ataaaaatta caaaaaaatc gatatataat
     6541 acaaaacact ttgactcagc aaaaaaatac acaatttata cgcttacaaa atacatatta
     6601 gtaatcatca tgatgattat tatcctggaa attttaggac tgaatttatc tgttctccta
     6661 atcggttcgt ctgccatctt agttggcgta ggcttaggaa ttcagaactt atttaatgat
     6721 tatatttccg gaattatcat tttgctggat tctacagtga aagttggtga tatcatcgaa
     6781 accaatggca taatttgcac aatacaacaa atcaatctgc gtactacaca agccattaca
     6841 agggaagaca aatatatcat tcttccaaat tcattgttga ctaaaagtga aattgtaaac
     6901 tggactcatg gtgaacgaga ttcaagattt gaaatatcta ttggagtatc ctataatgct
     6961 gacataaaat ttgctatgca actaatgaca gatgctgcta ctgaacacga taaggttaat
     7021 aaaacaccca ttccaatagc tcgtttagcc gattttttag actcttctat tcagctgact
     7081 ttattatttt ggttgaatga tacgttcagg gcagaaaatt ataagagtca gattcgcatg
     7141 tccatttatg aaaagttcaa acaaaacaat attgaaattc ctttcccgca ggtagttata
     7201 caccgttctg ataaataaac aaatgatgaa aagtattaac gagatacaag acgaaattat
     7261 tgacgatttt tccatgtatg aagattggaa tgacaaatac gaattcctta ttgactatgg
     7321 taaatcatta caacctttgg atgaaaaata taaaaccgaa gaaaacagaa ttcatggctg
     7381 tcagagccgg gtttggctgc atgccgaaaa aaaagaagat aagctgttct tctacgcaga
     7441 ttctgatgcc atcattacaa aagggattgt aggcttattg gtgaaagttt tgagcggaca
     7501 ttctccaaaa gatatagctg aagctaattt gtactttata gaaaaaatag gattaaaaga
     7561 acatctctca ccaacccgtg caaacggctt agtgaatatg attaaaaata tgaaattaga
     7621 agcagttaaa aatataaacc attaagaagt taagttacat gaagggcatt cttaattatc
     7681 ttaatgactt aatggttaaa aaaaacagat acaatattaa aaagtgaacc attaagaagt
     7741 taagttacat gaagggcatt cttaattatc ttaatgactt annnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn natggttaaa aaataaaagt ctatgactaa aaaacaaatt
     7921 acacagttat cgtatgagat tattgggtac gcaataaaag tgcacaaagc actaggacct
     7981 ggattattag aaagcgttta tgccacttgt ttaaaatatg aattagaaaa aaacaaacat
     8041 caagttgtaa aagaattatc agtacccgtc tactacgata atattattat gaataccgaa
     8101 ctcagattag atttacttgt ggatgatatg ataatcgtag aacttaaagc tatagaaaat
     8161 atacttcctg tacacgaagc acaattattg acttacatga aattattaaa gaaaccacaa
     8221 ggattactaa ttaactttta taccgataat gttacaaaat ctatgaagcc ctttgtaaac
     8281 gagtatttta gtgagctaga agatgaataa caagtaaatt aagataaaat aaaaaataga
     8341 atgcaagata aatcattcat ggaaataaaa gacgaggcta tcagtcagat acaaacgatc
     8401 tatgaccctg agatacccgt aaatatatac gaactgggat taatctacga agtgaatgta
     8461 gatatggaaa accatgtgca tgtactaatg acacttaccg cacccaattg cccggctgca
     8521 gagagcctgc cggtagaagt agaacaaaaa ataacagcaa tcgaaggtgt caaaaactgc
     8581 aaagtagaaa ttacattcga tcctccatgg gatcaggaaa tgatgagcga aatcgcaaaa
     8641 ttagaattgg gattcctgta aaaaaataaa ctatttccta ataaattgtg aacgctccag
     8701 agtccttgtc ttcatcatct ttcataaaat tgcctttgga tggtgctgtc ttatagctgg
     8761 tcatctcttc cagttcgtca aacaagccac cggcatcata gaagcgaccg aagtttttat
     8821 tgaagcgcaa aggcactacg ccggtaggtc cgtttctgtg tttcgctatg atgacttctg
     8881 ccacaccttt taatggttca cctgaactgc cctcattcat attataatat tccggccgat
     8941 acaagaacat caccatatca gcatcttgtt caattgagcc cgactcacgt aagtctgata
     9001 actgcggttt cttttctccg cctcgcgttt ccacactacg gtttagctgt gataatgcaa
     9061 tcaccggaat attcaactct tttgccagcc cttttaatga gcgcgaaata gaagaaatct
     9121 cttgctcacg gttcatccct ttgtcgccgc cgccactcat caattgcaga tagtcgataa
     9181 tcaccatctt gatgccgtgt gcattttgca atcgccgaca ttgcgctcgc agctcataga
     9241 tattaatagc aggctgatcg ttgatataaa taggagcagc cgataaatgt tccactttat
     9301 cacgaagtaa agcatattca tcatcactca aacgaccatt ggcaatcttt tgtgcatcca
     9361 gttcacattc cagcgcaata aaacgtttgg ccaactgcac cgcacccatc tccaaagaga
     9421 atatagccac cggcatatta tggtcaacag cgccatttct ggcaaggttt aatacaaatg
     9481 ccgttttacc cattgaagga cgagccgcaa taattatcaa atccgttgct ttccaaccgg
     9541 aggtcatttt atccagctcg atatagccgg aaggcaccga atctgccagc aaaccgtctt
     9601 cacggttgag caaatcatca atctgcaaaa tagattttga aaccaattgc ccgattcctt
     9661 gcgtagagtt tcgcaagttc ttatcggtga tttcataaat gcgcttttca gcagtatcga
     9721 gcaaatcgaa aacatctgtc gtatcctcat aagcatctcg gataatgtcg ttggaaatgc
     9781 gtatcagttc tcgttgtata aatttctgga taacaatgcg cgcatgatat tccacatttg
     9841 cagaagacgc aattttgttg gttaattctg acagataaaa cgcaccgcca acttcttcta
     9901 attttccatc cttacgcaat tcttccgtga cagtaagcaa atctatag
//
LOCUS       DAOQII010000074         9844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3629_length_9844_cov_4.895801, whole genome shotgun sequence.
ACCESSION   DAOQII010000074 DAOQII010000000
VERSION     DAOQII010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_9844_cov_4.895801"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLF48_05095"
     CDS             complement(<1..340)
                     /locus_tag="PLF48_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48735.1"
                     /translation="MYKLSAVSYLNTKPFLYGLEHAAIADETEISLDPPAVTAEKLIH
                     NEVDIALIPVAVLPLLKQPKIISPFCIGSNGKVATVCLFSDVPLEEIETILLDYQSRT
                     SVQLVQILCRE"
     gene            complement(345..956)
                     /locus_tag="PLF48_05100"
     CDS             complement(345..956)
                     /locus_tag="PLF48_05100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNF48736.1"
                     /translation="MKNIYIIRHGQTDFNVRLIIQGRGINSDLNDEGLKQAKAFFDKY
                     HPVDFDVVYTSALKRTHQTVSHFIAQNIPHEIRDNIDEISWGDFEGKEHEESLKKTFY
                     AIVDEWRKGNLTIKIDGGESAQDLADRLMPFVEEIKNSTHDNILVCTHGRTLRVLMCL
                     LTGKSVSKMDTFEHTNTCLYKLRYDGTLFHLELENDIVHLSSN"
     gene            complement(1038..3614)
                     /locus_tag="PLF48_05105"
     CDS             complement(1038..3614)
                     /locus_tag="PLF48_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNF48737.1"
                     /translation="MSDLENKDHITTIDGLYEDWFLDYASYVILERAVPLLYDGLKPV
                     QRRIMHAMKEMDDGRYNKVANIIGSTMQFHPHGDASINDAIVNLGQKDLLVDCQGNWG
                     DIRTGDSAAAPRYIEARLSKFALDVLFNEDTTEWQLSYDGRKREPIALPVKFPLVLAQ
                     GTEGIAVGLSTKIMPHNFCELIDASIKYLRKEPFELFPDFQTGGMVDVREYNDGKRGG
                     RIRVRAKIEVVDKKFLKITEIPFGTTVPDLIDSVVKANEKGKIKIKRITDNTAKDVEV
                     IIELQPQVSTSVAVDALYAFSDCEMSISPNTCVIIDDKPHFIGVTEALKISTDTTVQL
                     LKLELEILLKELEDKWHFSSLEKIFIENKIYRDIEEEETWEGIIQAIDKGLDPFKKLL
                     MREVTQDDIIRLTEIKIKRISKFDKKKADEYIKGLENEMEETKKNLKSLIKYAIRYFE
                     DLLKKYGKGKERKTQIMLFDTVEVKQVAANNEKLFVNRSEGFIGYGKDMRKEEFVCEC
                     SDIDDIIVFTKESVMKVVRNADKVFVGKDILHIAVWKKNDERTTYNAVYLDGKSGISY
                     AKRFNVTSITRDKEYFISQGNPKSKMLYFSVNPNGETETCSVILSPNCSARNKVFEFD
                     FFNLDIKNRGSIGNIVSKYPVKKVELKSKGKSTLGGVEIWLDEDIGRLNTDKRGRYLG
                     SFQNDDRILVVLKSGEYYLTNYELTNRYNMQDVWAIDKLYPSTTISAIHYSDSKKCNY
                     VKRFQIETTTLDTKNSFIIDESGAKLVFATLFTKPIVEYSLDLGKGKTKEETVSLAEF
                     IDIKGWKAIGNKLIGGTIKSVKLLEMPTSENEEQQVNFDVDFEITNLTDKKEPEQGEL
                     FD"
     gene            3836..4849
                     /locus_tag="PLF48_05110"
     CDS             3836..4849
                     /locus_tag="PLF48_05110"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF48738.1"
                     /translation="MQKKIVITGASGFIGYNLLEASLKRDYQVTAFLLPGDALAETLK
                     AKKVHVVFGDIRNLEDVINAIPQNTDIVFHCAAYVSDWGKRELFEAITVNGTENVCKA
                     SVAANVKRLVKISTNDVFGIDESVVIDETHPLHKWNEPYPDTKLKAEEIAWSYHKNHS
                     LQVTMVYPCWVYGPGDKTFVPLLADAILKREMVFFRKNALVWPSYVDNVVDLMLHISE
                     HENAVGNGYLVHDGESDTLENFTNKIATHISAKKVTTYIPYFLAYWAAVVMEFFGKLF
                     DKKERPLLTTYTVKNLGSRLRFSIEKAIKELDWKPKTSYTEGFANTMEWLDKQNKNEL
                     KQK"
     gene            4846..5643
                     /locus_tag="PLF48_05115"
     CDS             4846..5643
                     /locus_tag="PLF48_05115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF48739.1"
                     /translation="MNYENKLAFITGGSTGIGLAIATALAIEKCTVVLFARDTTKLQQ
                     AKDAIQNKVSSSVHIFSADMSDYSAAKLVLEEAVAQIGIPDILVNCVGIARPDYFENI
                     SPQQFDFTIKTNLYSMWNSNSILVPYMKKRGGHIINTSSIAGFIGVFGYTDYSMTKFG
                     IIGFSEALRSELKKYKITVQVLCPPDTDTPGFEEENINKPEETKAIGGKAKLMQPETV
                     AKEVLQKLGKNTFILLPGFDGKMSWRLKRFFPKLIEWIMDNSINKAK"
     gene            5801..6667
                     /locus_tag="PLF48_05120"
     CDS             5801..6667
                     /locus_tag="PLF48_05120"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNF48740.1"
                     /translation="MASFHPKDKNIKQISNFLTSAIIPRPIAFVSTIDKDGNPNLSPF
                     SFFNVFSSIPPILIFSPAKSALTGNQKDTYHNVKEVPECVIHIPTADMVEQLSVTSGT
                     YPKGINEFVKGGFEEGKSALVKPPRIKDAPLAFECKVNQIIELGTEGGSGNLVICEIV
                     YVHFDENKVDENGNMIAEKLNPIARLGYNNYVSFTKENIFETPKPLRHFGVGVDAIPQ
                     WLKDEQMLNGNELGKLASISELPNAADINQYSELTELLNDFPQQMYSDIKRYLENNQV
                     IEAWLIILNARK"
     gene            complement(6719..7294)
                     /locus_tag="PLF48_05125"
     CDS             complement(6719..7294)
                     /locus_tag="PLF48_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNF48741.1"
                     /translation="MVRWIFILIFKTLGWKLKTEAIVGIKQSITIAAPHTANMDLPIA
                     KAAFDLMGLPLRFTVKKEWFRFPFKKLTASLGGIAIDRSPKKPGEPRPSMVEAMVQLF
                     KENPELHIMVTPEGTRSKRTEWKTGFWYTAKQANVPILLGYLDYKYKIAGIGKIIYPT
                     NLEDDMRTMMEFYKDITPCHPDKFSVDLRYI"
     gene            7480..8709
                     /locus_tag="PLF48_05130"
     CDS             7480..8709
                     /locus_tag="PLF48_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNF48742.1"
                     /translation="MSDFIKPFEEKAPEIVFEWHDAKTGAEGWLVINSLRGGAAGGGT
                     RMRVGLTRHEVVSLAKTMEIKFSVSGPAIGGAKSGINFDPNDPRKREVLERWFKAVSP
                     ILKNYYGTGGDMHIDEIHEVIPITEEYGIWHPQEGIVNGHYHPKERDKINKIGQLRVG
                     VSKLIEDEDYTPSKKEGFLVADMITGYGVAESVKHYYKLYQNRSVENKKVTVQGFGNV
                     GAAAAYFLAQQGAKVVGIIDTTGAFINEDGFSFDEIKMLLLHRRDRKLFHEDLIPFEH
                     AVKDFWKLKTDIFIPAAGSRLIGEKELNEMIKSGVEVISSGANVPFNDPEIFYGKILN
                     KADENIAVIPDFIANCGMARVFAYLMGKHVKITDDAIFQDVSKTIYKALEKVHQKDKA
                     ERFITRNALQIAIEELS"
     gene            8875..>9844
                     /locus_tag="PLF48_05135"
     CDS             8875..>9844
                     /locus_tag="PLF48_05135"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNF48743.1"
                     /translation="MPIYHHLGKIPQKRHIVFQSDAGKHYYEELFGTEGFNSHSSLLY
                     HIHRPTQVREILSSKDVTPKIAIEKNIKSLLLEGFKIEAQDDYLDSRKTVLFNNDCCI
                     ILAAPKKSMHDYFYKNADADEMLFIHKGSGTLRTMLGNIPFQYGDYLIVPRGMVYQIQ
                     FDTEDNRLLIMESYAPVHTPRRYKSSSGQLLEHSPFCERDFILPQNLETHDETGDFIV
                     KIKKQNQLYDFVYATHPFDVVGWDGYNFPYGFSIHNFEPLTGRVHQPPPVHQTFETSA
                     LVVCSFVPRLYDYHPRAIPAPYAHSNIDSDEVLYYVDGDFMSRNNIK"
BASE COUNT         3112 a         1780 c         1864 g         3088 t
ORIGIN      
        1 attctctgca taaaatttgt accaattgca cagatgtgcg cgactggtaa tccaagagta
       61 tcgtttcaat ttcttccaaa ggtacatcag aaaaaagaca aaccgtggct acctttccat
      121 tactgccgat acaaaaagga gaaattattt taggttgttt taataagggt aatacggcaa
      181 cgggaattaa tgcgatatca acttcattgt gtatcagttt ttcggcagta actgccggcg
      241 ggtctaatga aatttcagtt tcatcagcta ttgcagcatg ttccagtccg tataaaaatg
      301 gcttggtatt gagataagaa acagcagaca gtttatacac gtaattaatt tgaagataaa
      361 tgtacgatat cgttttctaa ttccagatga aataaagtgc cgtcgtatcg aagtttatat
      421 aagcaagtat ttgtgtgttc aaaggtatcc attttagaaa cagattttcc ggtcagcaaa
      481 cacatcagta cgcgcaaggt acgcccgtgt gtgcaaacca aaatattatc gtgtgtagaa
      541 tttttgattt cttcgacaaa tggcattaag cgatcagcca aatcctgtgc actttcgcca
      601 ccgtcaattt tgatggtcaa attacctttt cgccattcat caactatcgc ataaaaggtt
      661 tttttcagac tttcttcatg ttctttacct tcaaaatcac cccaactgat ttcatcaata
      721 ttatcccgga tttcatgcgg aatgttttga gcaataaaat gagaaacggt ttgatgtgta
      781 cgcttcaatg cggaagtata aactacatca aaatctacgg gatggtattt gtcgaaaaag
      841 gctttagctt gtttgagtcc ctcgtcattt aaatcagaat ttatgcctct accctgtatg
      901 ataagcctga cgttaaagtc tgtctgacca tggcggataa tgtaaatatt tttcacccta
      961 taaaaataag catagtatca cgaagttgaa tgtgattttt aagtaaagac cataacgcac
     1021 ttcctgttaa attggaacta atcgaacaat tcgccttgct cgggttcttt tttatcggta
     1081 aggttcgtaa tctcaaaatc aacatcaaaa ttgacttgct gttcttcatt ttcagaggtt
     1141 ggcatttcga gcaattttac actttttatt gtaccgccga taagtttgtt gccaatggct
     1201 ttccatcctt tgatgtcaat aaattcagcc aaactaaccg tttcttcttt cgtttttcct
     1261 tttcctaaat ctaaggaata ttctactata ggttttgtaa ataaggtggc aaaaaccagt
     1321 ttggcaccgg attcgtcgat gataaaactg tttttcgtat ctaatgtagt agtctcaatt
     1381 tggaaacgct taacgtagtt gcattttttg ctgtctgaat aatgaatggc tgaaatagta
     1441 gtagacggat acagcttgtc tattgcccat acatcctgca tattataacg attagtaagc
     1501 tcgtaattgg ttagataata ttcacccgat tttaacacta cgagaattct gtcatcgttt
     1561 tggaaagaac ccaaatacct gccgcgtttg tctgtgttca gcctgccaat gtcttcatcc
     1621 agccaaattt ccacaccgcc taaagtagac ttgccttttg atttcaattc cactttttta
     1681 acaggatatt tggaaacaat atttcctata gaccccctgt ttttgatatc cagattaaaa
     1741 aaatcaaatt caaatacttt gttgcgggca ctgcaattcg gtgataaaat aacactacag
     1801 gtttcagttt ctccattcgg attcactgag aaataaagca tcttggattt cggatttcct
     1861 tgagagataa aatattcttt atcacgtgta atagaagtta cattaaaacg cttggcataa
     1921 ctgatgcctg attttccatc cagataaacg gcattatagg tggtgcgttc atcattcttc
     1981 ttccatacgg caatatgcaa tatatcttta ccaacaaata ctttatcagc attgcgcact
     2041 actttcatca cggattcttt cgtgaagact ataatatcat ctatgtccga acactcgcat
     2101 acaaattctt ctttgcgcat atctttgcca tagccgataa atccttccga acggttgaca
     2161 aataactttt cattattggc agccacttgt tttacttcca ctgtatcaaa cagcatgatt
     2221 tgcgttttgc gctcttttcc tttgccgtat ttttttaata aatcttcaaa atagcgaata
     2281 gcatatttaa taagtgattt gagatttttc ttggtttctt ccatttcatt ctccaatccc
     2341 ttgatgtatt catcggcttt ctttttatcg aacttggaaa tgcgctttat cttgatttca
     2401 gttaaacgaa ttatgtcatc ttgcgtaacc tcacgcatca gtaatttttt gaaaggatct
     2461 aatcccttat caatagcctg aataatacct tcccatgttt cttcttcttc aatatcgcgg
     2521 taaattttat tttcgataaa tattttttca agtgaagaaa aatgccattt gtcttccagt
     2581 tctttcaata aaatttccaa ctctaatttc agtaactgaa ccgttgtatc tgttgatatt
     2641 ttcaaggctt cggtaacacc tatgaagtgc ggtttatcat caataatgac acacgtattt
     2701 ggtgaaatag acatttcgca atcagaaaat gcatacaacg catctacggc tacacttgtc
     2761 gacacttgag gttgcagttc aatgataact tctacatctt ttgctgtgtt gtcggtaatg
     2821 cgtttgatct ttatcttgcc tttttcattg gcttttacaa ctgaatctat taaatcaggt
     2881 acggtagtgc cgaaaggaat ttctgtaatt ttgaggaatt ttttatcaac gacttctatc
     2941 tttgctctta ctctgattct tccgccgcgt ttcccatcat tgtattcacg cacatccacc
     3001 atgccacctg tttgaaaatc tggaaacaat tcaaacggct ccttgcgcaa gtatttaatg
     3061 gaggcatcaa ttaattcgca aaaattgtgc ggcataattt tagtagataa acccactgcg
     3121 ataccctctg taccttgtgc caaaactaag ggaaatttta cgggcaaggc tatcggttca
     3181 cgtttacggc cgtcataaga taattgccat tcggtagtat cttcattaaa taaaacatcc
     3241 agcgcaaact tgcttaaacg tgcttctata tatcgaggtg ctgcagctga atctccggtg
     3301 cgaatatcac cccagttgcc ctggcaatcc actaataaat ctttttgtcc cagattaact
     3361 atggcatcat tgatactggc atcgccatgt ggatggaact gcatggtaga accaatgata
     3421 tttgccactt tattataccg cccgtcatcc atttctttca tcgcatgcat aatacgacgt
     3481 tgtacaggtt ttaatccatc atagagcagt ggtacagcac gttccaaaat cacataggac
     3541 gcataatcta aaaaccagtc ttcataaagt ccgtcaatcg tggtgatatg atctttattt
     3601 tccaaatcgc tcattacata tcggtttagc ctgcaagtat aaggcaatcc tgataaaatg
     3661 agaaatagag ttttaaacgg gtgtggttaa tttattcaac aaatttgtgc ttacattaag
     3721 ctgtttgaag agcaaagatt gaatctattt atgagtagct aagagaatgc cgtgcaatta
     3781 cttcagcact taatttgttt ttcaaataat ttagcctatc ttttcctgat agattatgca
     3841 aaaaaaaata gtcatcaccg gagcatccgg ctttataggt tataatctgt tggaagcatc
     3901 actgaagaga gattatcagg ttacagcctt tttactaccc ggtgatgctc ttgccgaaac
     3961 cttaaaagcc aaaaaagtac atgtcgtttt tggtgatatc cgcaatttgg aagatgtgat
     4021 aaacgctatt cctcaaaata cagatattgt atttcattgt gctgcttatg tgtcagattg
     4081 gggtaaacgg gaactctttg aagcaattac cgttaacgga acagaaaatg tgtgtaaggc
     4141 tagtgtagcc gcaaacgtaa aacgattggt gaaaatcagc accaatgatg tatttggcat
     4201 agatgaatct gttgtcatag atgaaacaca tcctttgcat aaatggaatg aaccttaccc
     4261 ggatacaaaa ttaaaagcgg aagaaattgc atggagttat cataaaaatc atagtttaca
     4321 agtgacgatg gtttatccat gctgggtata tggaccgggt gataagacct ttgtgccttt
     4381 gcttgcggat gccattctga aaagagaaat ggtgtttttt agaaaaaatg cattggtttg
     4441 gccaagctat gtagataatg tggtggattt aatgctgcat atttcagaac acgaaaatgc
     4501 agtgggcaat ggctatttgg ttcatgacgg agaaagtgat accctcgaaa attttaccaa
     4561 caaaattgca acacacatca gtgctaaaaa agtgactacc tatattcctt attttttagc
     4621 ttattgggca gctgtggtaa tggagttttt cggtaaatta tttgataaga aagaacgacc
     4681 tttgttgacc acttacacag ttaaaaattt aggctctcgc ttgcgatttt ctattgaaaa
     4741 agcaatcaaa gaattggact ggaagccgaa aacaagctac actgaaggat ttgcaaatac
     4801 gatggaatgg ctggataaac aaaataaaaa cgaactcaaa caaaaatgaa ctacgaaaat
     4861 aaattggcat ttattacagg cggttccacc ggcattggtt tggctattgc aacagcgttg
     4921 gccattgaaa aatgcacagt tgtacttttc gcccgagata ctacaaaatt acaacaagct
     4981 aaagatgcta ttcagaataa agtatcttct tcagttcata ttttttcggc tgatatgtcg
     5041 gattatagtg ccgctaagtt ggttttggaa gaagccgtag cccaaattgg tattccggat
     5101 atcttagtga attgtgtcgg cattgcccgc cctgattact ttgaaaatat tagtccgcag
     5161 caatttgatt tcacgataaa gaccaattta tattcgatgt ggaacagcaa cagcatcctt
     5221 gtgccttata tgaaaaaacg aggagggcat atcataaata cttcatctat tgcaggattt
     5281 ataggcgtgt ttggctatac cgattattcg atgacgaaat ttggaattat cggtttttca
     5341 gaagcattgc gcagcgaact caagaaatat aagataacag tacaggtgct atgtccgccg
     5401 gatacagaca cgccgggttt tgaggaagaa aatataaaca agccggaaga aacaaaagca
     5461 ataggaggga aggcaaaact gatgcagccg gaaacagtgg caaaggaagt gctgcaaaaa
     5521 ctgggtaaaa atacatttat tctactaccc ggttttgacg gcaaaatgag ctggcgttta
     5581 aagcgttttt tcccaaagct gatagagtgg attatggata atagcattaa taaggcaaaa
     5641 taataaggca aatcttataa tgtataattg agacaggatt taaaggaatg ttgtgcaaat
     5701 tgataataag actgttattt ttgtaaaaaa gttacacaga atagaatgcg tttttcttta
     5761 aggtaattac ttttatttct taaacaataa aatactaata atggcatcat ttcatccgaa
     5821 agataaaaat atcaaacaga tttcaaattt cttgacttca gcaattattc caagaccgat
     5881 tgcatttgta agtacgattg ataaagacgg caatccaaat ttaagcccat tttctttctt
     5941 taatgtattt agctccatac cgccgattct gattttttcc cctgcaaaga gtgcattgac
     6001 cggtaatcaa aaagatacct accacaatgt aaaagaagta cccgaatgtg ttattcatat
     6061 acctactgct gatatggtag agcaactatc tgtaacgagc ggcacttatc ccaaaggaat
     6121 aaacgaattt gtaaaaggtg ggtttgaaga aggaaaatct gctcttgtta agccaccccg
     6181 aattaaagat gcaccattgg catttgagtg taaagtaaat caaattattg aattgggcac
     6241 ggagggtggc tctggtaatt tagttatttg tgaaattgtg tatgtccatt ttgatgaaaa
     6301 taaagtagat gaaaacggaa atatgattgc agaaaaacta aatcctattg ccagattggg
     6361 ttacaataat tatgtttcat ttaccaaaga aaatattttt gaaacgccta agccgcttcg
     6421 gcattttggt gtaggagttg atgccattcc tcaatggttg aaagatgaac aaatgctgaa
     6481 tggaaatgaa ttaggaaagc tggctagtat cagtgagttg cccaatgcag cggatatcaa
     6541 tcaatactca gaattgacag agctgttgaa tgactttcca caacaaatgt attcggatat
     6601 aaaaagatat ctcgaaaata atcaagtgat tgaagcttgg ttgattattc tgaatgctag
     6661 aaaataatac ctgacaaaaa tgtataacac agtttgcgtt tatctgcgca attagttttc
     6721 aaatataccg taagtccaca ctgaatttat ccggatgaca cggtgtaatg tctttataaa
     6781 attccatcat cgtgcgcata tcgtcttcca gattagtagg ataaataatt ttgccaatgc
     6841 cggcaatctt gtatttataa tctaaataac ctaataatat aggaacgtta gcttgttttg
     6901 cagtatacca aaatcccgtt ttccattctg tgcgtttgct gcgagttcct tcgggtgtta
     6961 ccatgatatg caattccgga ttttctttaa atagttgcac catagcttcc accatgctgg
     7021 ggcgtggttc ccccggcttt ttggggcttc tgtctatggc tattccacca aggcttgctg
     7081 tcagtttttt aaatggaaac ctaaaccact ctttttttac cgtaaaccgc aaaggtaaac
     7141 ccattaaatc aaaagctgct ttggctatcg gtaagtccat attggcagta tgaggagctg
     7201 caatggtaat cgactgtttt atgccaacaa ttgcttctgt ttttaatttc cagcctaatg
     7261 tcttaaaaat gagtataaaa atccaacgta ccatagtaat acaaagatgc acatttaatt
     7321 ttgtttttta attcgttata aatcggataa ggtaatgaga cgaccaaaga agagaagaaa
     7381 ataattttaa ataaattagt tgctatgaca actaattttt tctatcttta ttttagaata
     7441 caatcttaat actaattact tactacttga tactatttta tgtcagattt tattaaaccc
     7501 tttgaagaaa aagcacctga aatcgttttt gaatggcatg atgccaaaac tggagctgaa
     7561 ggctggttgg tgataaattc tttgcgcgga ggtgctgccg gtggcggtac ccgtatgcgt
     7621 gtaggattga ccagacacga agttgtttcg ttagctaaaa cgatggaaat taaattttca
     7681 gtttctggac ctgctattgg cggggcaaaa tcaggcatta atttcgaccc gaacgatccc
     7741 cgaaaacgag aagttttaga acgctggttt aaggcagtat cacctatctt gaaaaactac
     7801 tatggcaccg gcggagatat gcatattgat gaaattcacg aggtgattcc aattacggaa
     7861 gagtatggga tctggcatcc gcaagaaggc attgtaaacg ggcattatca cccgaaagag
     7921 cgtgataaga ttaataagat aggacaactg cgcgtgggcg tctccaagct tattgaagat
     7981 gaagattata caccatccaa gaaagaaggc tttttagtag cggatatgat taccggttat
     8041 ggtgtggcag agtcggtaaa acattactac aaattatatc aaaacagatc agtagaaaat
     8101 aaaaaagtta cggtacaggg ctttggtaac gtgggtgccg cagcggcgta ttttttggca
     8161 caacaaggcg caaaagtggt aggaattatt gacactaccg gggcatttat caatgaagac
     8221 ggattttcat ttgatgaaat aaagatgctc ttgttacaca gaagggatag gaaattattt
     8281 catgaagatt tgataccgtt tgaacacgcc gtgaaagatt tctggaaatt aaaaacagat
     8341 atttttattc ctgctgctgg ctcaagatta atcggagaaa aagaactgaa tgaaatgata
     8401 aaatcaggag ttgaagttat ttcctcaggt gcaaatgtgc cgttcaatga tcccgaaatt
     8461 ttttatggta aaatattgaa taaagctgat gagaatattg ccgtcatccc tgattttatt
     8521 gccaattgcg gtatggcaag ggtatttgcc tatctcatgg gtaagcatgt gaagattaca
     8581 gacgatgcca tatttcagga tgtatcgaaa actatttata aagctctcga aaaggtacat
     8641 caaaaagata aggcagaaag atttatcacc agaaatgctt tacaaattgc catagaagaa
     8701 ttaagttaat gtaaggatgt agcaattcaa taatgtaata atgaggaaaa ttaaatcgtg
     8761 ataattcgtg aaaatgtttc attcgtattt aaatatatta tttaataatg ataaaattac
     8821 tactacaaat tcattgctat attgttaaat tatttaatta ataaattata aaacatgcct
     8881 atatatcacc atctcggaaa aattcctcaa aaaagacata tcgtatttca gtctgatgca
     8941 ggaaaacatt attacgaaga gttattcggt acggaaggat ttaacagcca ttcgtccttg
     9001 ttatatcata tccaccgccc tacgcaggta agagaaattt tatcctcaaa agatgtaaca
     9061 ccgaaaattg cgatagagaa aaatatcaaa tctttattgc ttgaaggttt caagatagaa
     9121 gcgcaggatg attatttaga cagccgaaaa actgtattat tcaacaacga ttgctgcatc
     9181 atactggcag ctcccaaaaa gtcaatgcat gactattttt acaaaaatgc ggatgccgat
     9241 gaaatgctct ttatacataa aggcagtgga acattgcgca ccatgttggg caatatcccg
     9301 tttcagtatg gcgattatct tatagtgccc cgtggtatgg tgtatcaaat ccaatttgat
     9361 acagaggaca atcgtttgtt gattatggaa tcttatgccc ctgttcatac accacgacgt
     9421 tataaaagtt catcagggca actactggaa cactcgcctt tttgcgagcg cgattttatc
     9481 ttgccacaaa atctggagac gcatgatgaa acgggagatt ttattgtcaa aataaaaaaa
     9541 caaaaccagc tctatgattt tgtatatgcc acacatccgt ttgatgttgt tggttgggac
     9601 ggatataatt ttccttatgg tttcagtatt cacaattttg aacctttgac aggcagagta
     9661 caccagccgc ctccggtaca ccaaacattt gaaaccagtg ccttggtcgt ctgttcattt
     9721 gtgccacgct tatacgatta tcatccgcgg gcaatacctg caccatatgc acacagcaat
     9781 atcgatagtg atgaagtgtt gtattatgtg gacggagatt ttatgagcag aaacaacatc
     9841 aaac
//
LOCUS       DAOQII010000075         9838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3633_length_9838_cov_6.017275, whole genome shotgun sequence.
ACCESSION   DAOQII010000075 DAOQII010000000
VERSION     DAOQII010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_9838_cov_6.017275"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..859)
                     /locus_tag="PLF48_05140"
     CDS             complement(5..859)
                     /locus_tag="PLF48_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48744.1"
                     /translation="MAKGVVLDCMNGEKHLNKKGNPKETHGIDFLYSYLNPLVSGQLN
                     NVLISSKFETSKYPNSPTVKFKNFMEDIITTMECYDVSNVKDETLTGFSYSTLNDVGV
                     LFWLNNKADSEDDLIKFVANSRIEDTSVNKSIYIMDNKRVAFILEVMKFVKTKENDFE
                     SSFYYPNTGQNINPLERINVGKRLPVEYLNSSIIPIKLSNKNNSKETYLLLATFENFE
                     EGDLMRLMGLAKDIGTELVGKVIIAFADYNELEHKQTVTLAKQKFQSSEFTKNVSVIN
                     FLDPSSVF"
     gene            complement(942..1697)
                     /locus_tag="PLF48_05145"
     CDS             complement(942..1697)
                     /locus_tag="PLF48_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF48745.1"
                     /translation="MALEKFLQEIDVLKTEFDQYRNLDNAKIQEALAIEYTYESNRIE
                     GNTLTLQETQLVIEKGLTISGKPLKEHLEAINHKEAIDFIVDLVQNKEPLNERNLLQI
                     HSIILKSIDKENAGNYRKVPVLISGSTHTPPQPYLVQKLMEDYFLFYEENKNSLHPVV
                     LSAEMHERLVTIHPFIDGNGRASRLVMNLILLQNGFPIANIKGDTESRMKYYNALENS
                     RIDNKAAFILLIAELVKESLERRIGLVKNKTEL"
     gene            complement(1760..3136)
                     /gene="mnmE"
                     /locus_tag="PLF48_05150"
     CDS             complement(1760..3136)
                     /gene="mnmE"
                     /locus_tag="PLF48_05150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNF48746.1"
                     /translation="MHPDFSDTIIALSTPAGMGAIAVIRLSGEHAIRTTNELFKGKDL
                     QQQASHTLHFGKLMDGQEEVDEIVAAVFKSPHSYTKEDVVEISCHGSPYIVNKIITLF
                     LAKGVRLATPGEFTQRAFMNGRFDLSQAEAVADLIASDSQAGHKIAMQQMRGGISQKL
                     KTLRTALIEFTALLELELDFSEEDVEFADRSKFIQLLQDIRTEIATLAASFKLGNAIK
                     SGIPVAIVGKPNAGKSTLLNALLEEERAIVSSIAGTTRDTIEETLTIDGYLFRFIDTA
                     GLREANDEIEKIGIERSNAAIEKADIILYLFDLEFENEADFLAAFRERAGADKELIAI
                     GNKADKISETALQGILEKHPEFLALSAKNKAGIAELKHKLVAGIALKNKNENSVLLTN
                     QRHYDALLKAEAAIDNVQEMMRHNIPTDLLTQDIKIALRHIGEITGDIDIDKDILGTI
                     FGKFCVGK"
     gene            3271..>3570
                     /locus_tag="PLF48_05155"
     CDS             3271..>3570
                     /locus_tag="PLF48_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48747.1"
                     /translation="MKNLKIALLGVLFFSMTSLISAGENKSNTKSTSGTLKTSQRIAA
                     DKFLKDWSLTLHGGTTSPFTDIRSYDWVRQTKKPSELQWGAGISLTKMCGPVFGVQ"
     assembly_gap    3571..3670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3671..4617
                     /locus_tag="PLF48_05160"
     CDS             <3671..4617
                     /locus_tag="PLF48_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF48748.1"
                     /translation="KGYTNKFTEVVFPLAMGMKIKCNKSFDIGIEGNFVFTNTDKLDA
                     YSAHSDAVLASNPNGSTLKKMNRDAYAYLNVGVTYKFGRIGAQKEHNEWVNPLEAYMS
                     IIDSKIKDPFVVKDKDGDGILDELDQEPDTPEGAKVDSHGKTLDSDGDGIPDHLDKEP
                     FSTPMLPIVDGVNQHPKDSIIPQDIKQLINNEIKSGNTTAGWALSIIFFDLDKYNIKA
                     SEVPELYKVATVMQKYPDLKVYVKGYTDTRNTEAYNEKLSENRVNSAVDFLVKKFNIS
                     RDRFLPSHFGETDNIIPNATGEPEHHVNRRVEFTPANY"
     gene            4725..7193
                     /locus_tag="PLF48_05165"
     CDS             4725..7193
                     /locus_tag="PLF48_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNF48749.1"
                     /translation="MRRFYTLSVFLLLLFSANAQSISSLMGMLNSSGSSVTAASGSAQ
                     KAISQLATSGASGAAASQLQMLGLDPNIIQKYILKNEQAKNNSNNQASGDASAIPNPI
                     EDILGILMSIKTQQNDMSTVLAKLGIQDSTPAANVSPNDIYGHDFFGNQKLATFVKST
                     DARAADSYILDSGDELNIAVWGYADYNNKFKVNDDGYIQIPEFGRIYVRGLTFGAVKA
                     LIGKRLATFIDSKNTKYEISLIYSRTIDVNIVGEVKSPGTYQIPAINSVYNAINAANG
                     ITDIGSVRDIQIRRNGKVVKTFDVYEFLFNPLSDNNFYLQKGDFIFVGTQEKLVKIGG
                     AVRRPAKYELKKNEGLKNLVKFAGGFAPDANTKSLQIIRNNGKTQQIIDVNYDLLVSS
                     GSDYVLNDGDIVNVLAVTGDPENFVRIAGQIKYPGKYELKDGYRISDIIIAGGGITSD
                     AYLDRAYIKRKLPDNTYVNQKFSLKDIILNPSGSENLLLQKGDEIKIFSKDDFLEKFK
                     VSIAGSVINPITMDYTRGMTLNDLLFYAGGLKMEAANSKIEISRNVVSDTLKNEFSPQ
                     RITVLSASINDKLEIDEKSKSFLLEPFDQIFVRKTPGFNSQESLVINGEVMYPGIYPI
                     LDKNEKVLDLIKRTGGLTPYAYLNGATLVRLDNNQQEIIFELKNAFKDSTSRANLVLK
                     NGDVINIPTVNQQVSIKGNGIRYPDLDSNQTISGKFVPGKSARWYIKNYAGGFKKGAN
                     KRSTYVMYPNKKMDYTRFAMGIKNYPTVDYEGAVINVDMKVKKPKPPKGPESNINWNI
                     LLPSIIAGVTSLGTTLTLIFVLKN"
     gene            7198..8277
                     /locus_tag="PLF48_05170"
     CDS             7198..8277
                     /locus_tag="PLF48_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48750.1"
                     /translation="MQEEYFYEEDIRLVDILESFGNYFRYLFKKWYIFLPGVIGLTLC
                     GYFFATLSAPKFTANASFNAIDSRISSMGGLMSMMGISFSGGTSNDVLTGIFSSRNIF
                     YNSLLHDMITNEGTREKIGNVYMHTLKYDEGFAEDPNLKGFKFKANEISEMTKLELQI
                     ASMMYADFVDGSLDAEYDIATGLIKAEVESPEYELSKNLASNMLKATIDFYQQKQVEN
                     AKASYKNTDRRLDSINNEILIRQRRIAQNQDINIFNKKQISEIEVQKLSQELAVLNVM
                     YNDATNSKESAKAGLTPTNMVRIVDDPIFSVDEIHKGKILWSAIGFAASLILVVIPLL
                     LHKAVIDGREENKVRETRIAKMQTE"
     gene            8316..>9838
                     /locus_tag="PLF48_05175"
     CDS             8316..>9838
                     /locus_tag="PLF48_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HNF48751.1"
                     /translation="MGDDLNTNDLLQIDSIAIVGTACRLPGDVSHIEDLWQLVINKKD
                     GITDIPENRIPNFLSNIDAKRGAGKIVTAKGGFLKDIEYFDADFFAISPKESEKLDPQ
                     QRLLLETSFNAIEDAGISLEELNGSNTGVFIASWTNDFEHRLQQTGTDVDVYATTGTG
                     RYALSGRLSFFYNLQGPSLTVDTACSSSLVATHLAMQSLKSGECDIAIVGAANVILDT
                     FVSVGYSRSGLLSDYGGCYFGNKNPGGYVRSEGSAVIILKRTNDALRDGNNIYALLRA
                     SSCNNDGQAGRHMLSPGTATQEILMNSACKHAGINPSAIQYIEAHGTGTKAGDIAEIN
                     AIWRSFGQNRNQKLYIGSVKSNIGHTEAVAGFAGLFKLLMALKYKTIPPDIHLYEKNP
                     KINWEAMNIEIPLEALAWHQDSNSPIITGVNSFGVTGTNAFVLVQEYENIDTKQTNNP
                     FSTRILPLPISANSNKSFYSYIKSYDEIINESNWKNVISNIVYHKANLKYRKVILYN"
BASE COUNT         3148 a         1766 c         1740 g         3084 t
ORIGIN      
        1 cattctaaaa aacgcttgag gggtctaaaa aattgattac actaacattt ttagtaaact
       61 cacttgactg aaatttttgc tttgctaatg ttacagtttg tttgtgttct aattcattat
      121 aatctgcaaa tgctataatt acttttccta caagttctgt ccctatatct tttgccaaac
      181 ccatcaaacg cattaagtcg ccttcttcaa aattttcaaa agtggcaagt agcaaatacg
      241 tttctttaga attattctta ttgcttaatt ttataggtat tatagatgaa tttaagtatt
      301 caactggtag tctttttcct acatttattc tttctaaagg gtttatgttc tgccctgtat
      361 tagggtagta aaaagaactt tcaaaatcat tctcttttgt ctttacaaat ttcattactt
      421 caaggataaa agctactcgt ttattatcca ttatgtagat agatttattt acgcttgtat
      481 cttcaatacg agaattggca acaaatttta ttaaatcatc ttcactatca gctttattgt
      541 ttagccaaaa taaaacacca acatcattta aggttgaata actaaagcct gttaaagttt
      601 catctttaac atttgaaaca tcgtaacact ccatagttgt aataatatct tccataaagt
      661 ttttaaactt tactgttgga ctattaggat atttacttgt ttcaaatttt gaagaaatta
      721 aaacattgtt taattgccct gaaacaagag gatttaggta tgaatataaa aaatcaattc
      781 cgtgtgtttc ttttgggttt ccttttttgt ttaaatgctt ttctccattc atacagtcta
      841 aaacgacacc ttttgccata tcgtgccagc ctataacaga aaaaaacttt ctacgacatt
      901 ttcgccatac tcaccaattt ttttagacca ttcgcccata attacaactc ggttttattt
      961 tttactaatc caatcctcct ctctaaactc tcttttacca actccgctat caacaatata
     1021 aatgctgcct tattatctat tcttgaattt tccaaagcat tataatactt cattctgctt
     1081 tcagtatcac ctttgatgtt tgcaatagga aaaccatttt gaagtaaaat taaattcata
     1141 actaaccgtg aagctctgcc gtttccatca ataaaaggat gtatggtaac taatcgttcg
     1201 tgcatttcgg cacttaatac aactggatgc agactattct tgttttcttc ataaaacaaa
     1261 aaataatcct ccatcaattt ttgaactaaa tagggttgtg gtggtgtgtg ggtacttcca
     1321 cttattagga ctggtacttt tctataattc ccagcatttt ctttgtcaat gctttttaaa
     1381 atgattgaat gaatttgtaa taaatttcgt tcattcaatg gctctttatt ttgtactaaa
     1441 tcaacaataa aatctattgc ctctttatga tttatagctt ctaaatgttc ttttaaaggc
     1501 tttccgctaa tagttaaacc cttttcaata actaactggg tttcttgtag ggtaagggtg
     1561 tttccctcaa ttctgttact ttcgtaggtg tattcaatgg ctaatgcttc ttgaattttt
     1621 gcattatcca agtttcggta ttggtcaaat tccgttttta aaacatcaat ttcctgtaaa
     1681 aatttttcta aagccatttc cacaaatata atttattctt ctagtaaatt ttagaatttt
     1741 ataaatcagt tattacattt cacttcccca cacagaactt cccgaatatg gtgcccagta
     1801 tatctttgtc gatatcgata tctccggtta tttcaccgat atggcgcagg gcaattttta
     1861 tgtcttgtgt gagcaaatct gtgggtatat tatgccgcat catttcctgt acattgtcta
     1921 tggcagcctc tgctttcagt aatgcatcat agtgccgttg gttagtcagc agtactgaat
     1981 tttcgttttt gttttttagt gcaatgccag caaccagttt atgctttaat tcggctatcc
     2041 ctgctttgtt tttcgctgaa agtgcaagaa attcagggtg tttctcgagt atcccttgca
     2101 gtgcagtttc cgaaatctta tctgctttat ttcctatggc aataagctct ttatctgctc
     2161 cggctctttc acgaaaggct gctaaaaaat cagcttcatt ttcaaactcc aaatcaaata
     2221 aatacaggat gatatccgct ttttctattg ccgcattact tcgttctata ccaatttttt
     2281 caatctcatc gttggcttct ctaagccctg ctgtatctat aaacctaaac agatagccgt
     2341 ctatcgtcag tgtttcttct atggtatcgc gagtggtacc ggcaatgctg cttacgatgg
     2401 cgcgctcttc ttccagcagc gcattcagca aggtagattt tcctgcatta ggcttaccta
     2461 caattgccac aggaattccg ctttttatgg cattacccaa cttaaaagaa gctgccagtg
     2521 tggctatttc ggtgcgtata tcttgcagta gttgtataaa tttactcctg tcggcaaact
     2581 ccacatcttc ttcactaaaa tctagctcca gttcgagcag tgccgtaaat tctatcaatg
     2641 ccgttcgcaa ggttttcaat ttctgtgata tacctccccg catctgttgc atggctatct
     2701 tatgtccggc ttgcgaatct gacgctatca aatcggctac agcctccgct tgcgacaagt
     2761 caaaccgacc attcataaaa gcgcgttggg taaattcgcc gggtgttgcc aggcgcacac
     2821 ctttagccaa aaacaacgtg ataattttat tgacaatata tggcgaacca tggcaggata
     2881 tctcgacaac atcttctttg gtataagaat gtggggattt aaaaactgcg gctactattt
     2941 cgtctacctc ttcctgccca tccatcagct taccaaaatg cagggtgtgg cttgcttgtt
     3001 gctgtaaatc tttaccctta aacaattcat ttgtggttcg aatggcatgc tctcccgata
     3061 gacgaatgac agcaatagct cccatgcctg ccggagtaga aagtgcaatt attgtatcag
     3121 aaaaatcagg atgcatcacc tttgggtaaa attcataatt tttgattaaa aaattgagaa
     3181 attatataaa atatgggaca aaaagctcaa aaaatatttt tgtttctgta ttttcgtact
     3241 caaacctgtt atttatttta aaattagaca atgaagaatc taaaaattgc attgcttggt
     3301 gtattgtttt tctcaatgac gagtttaatc tcagcgggag aaaacaaatc taacaccaaa
     3361 agcacctcag gaacattaaa aacatctcag cgcatagccg cagataaatt tctgaaagac
     3421 tggtcactta ccctgcatgg aggtactact tcaccattta cagatatcag aagttatgac
     3481 tgggtacgtc aaacaaagaa acccagtgag ctgcaatggg gtgccggtat cagtctgaca
     3541 aagatgtgcg gtcctgtttt tggcgtgcaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn tgaaagggta taccaacaaa tttacagagg ttgtgtttcc tttggcaatg
     3721 ggtatgaaaa ttaaatgtaa taaatccttt gatattggta tagaaggtaa cttcgtattt
     3781 accaatacgg ataaactgga tgcctattcc gcacattctg atgcagtatt agcttccaat
     3841 cctaacggtt ctaccttgaa aaaaatgaac agagatgctt atgcatattt gaatgtaggt
     3901 gttacttata aattcggacg tatcggagct caaaaagaac ataacgaatg ggtaaatcca
     3961 ttagaggcct atatgagtat tattgacagc aaaattaaag acccatttgt agtgaaagat
     4021 aaagacggtg atggtatttt agatgaactg gatcaagaac cggatactcc ggaaggggca
     4081 aaagtagact cacatgggaa aacattggac agtgatggtg atggtatccc tgaccattta
     4141 gataaagagc cattttctac accgatgttg cctattgtag acggagtaaa tcaacaccct
     4201 aaagattcca tcattcctca agacattaaa caacttatca ataatgaaat aaaaagtggt
     4261 aatactacag caggatgggc actttctatc atattttttg atttagacaa atataatatt
     4321 aaagcatcag aagttccgga attatacaaa gtggcaacag taatgcaaaa ataccccgat
     4381 ttaaaggtct atgttaaagg ctatactgac acgagaaaca cggaagcata taacgaaaaa
     4441 ctgtcagaaa acagagtgaa ttctgctgtt gattttctgg taaaaaaatt caatatttcc
     4501 agagacagat tcctgccttc acatttcggc gaaacagata atattattcc taacgccaca
     4561 ggtgagccgg aacatcatgt aaacagacgg gtagaattta caccggcgaa ttattaaata
     4621 cttgtgagat aacataattt aaggggcaac cgaatgagtt gccctttttt tatatccatt
     4681 tctgactttt gtttactaca tttgtaacct ttatgactaa atccatgaga agattttata
     4741 cactttcagt ctttttatta ttattgtttt ctgccaatgc tcagagtata agtagtttaa
     4801 tgggaatgct taattcttca ggatcttctg taactgctgc aagtggctca gctcaaaaag
     4861 caatatctca gctagccaca agcggagcta gcggcgcggc agcatctcaa ttgcagatgc
     4921 ttggactgga tcctaatatt attcaaaaat atatcctaaa gaatgagcag gctaagaata
     4981 actcaaataa ccaagccagt ggggacgcca gtgctattcc aaacccaatt gaagacatat
     5041 taggtatttt aatgtctatt aaaacacagc aaaacgatat gtcaacagtc ctagcaaaac
     5101 taggcataca agacagtact cctgctgcaa atgtatcacc taacgatatt tatggacatg
     5161 attttttcgg caatcaaaag ctggccacat ttgttaaatc cacagatgca agagcagctg
     5221 attcttatat attagattca ggtgacgagt taaatatcgc agtttggggt tatgcagatt
     5281 acaacaacaa gtttaaggtt aacgatgatg gttatatcca aatccctgag tttggaagaa
     5341 tttatgttcg tggtttgaca tttggagcgg taaaagcact tatcggcaaa cgattggcaa
     5401 cattcattga ctctaaaaat acgaaatacg aaatatcttt aatttattcc cgtacgatag
     5461 atgtaaatat tgtcggcgaa gttaaatcac ccggcaccta tcagataccc gctattaatt
     5521 cagtttacaa tgctatcaat gccgcaaatg ggataactga tataggaagt gtccgcgata
     5581 tacaaatcag aagaaacgga aaagtggtta agacatttga tgtatatgag ttcttattta
     5641 atccgcttag tgataataat ttttacttac aaaaagggga ttttattttc gttggaactc
     5701 aagaaaaatt agtgaaaata ggaggggctg tgcgccggcc ggctaaatat gaattgaaga
     5761 aaaatgaggg actcaaaaac ttagtcaaat ttgcaggtgg ttttgctccg gatgcaaaca
     5821 ccaagagtct tcagattatt cgcaataatg gcaaaaccca gcaaattata gatgtaaatt
     5881 atgatttact ggtttcttcc ggttctgatt atgtccttaa tgacggtgat atagtgaatg
     5941 ttttagcggt aaccggagac cccgaaaatt ttgtaagaat agccggtcaa attaagtatc
     6001 cgggtaaata cgagcttaaa gatggctata gaatttcaga cattattatt gccggtggcg
     6061 gaattacatc tgatgcttat ttagacagag cctacatcaa gagaaaactt cctgataata
     6121 cctatgtcaa tcaaaaattt tccttaaaag atatcattct aaatccatca ggttctgaaa
     6181 atctgttact tcaaaaagga gacgaaataa aaatcttttc taaagatgat ttcttggaaa
     6241 aatttaaagt atcaattgcc ggttctgtaa ttaatcctat tacaatggac tatacaagag
     6301 gcatgacatt aaatgatctt ttattttatg caggtggatt aaaaatggaa gcggcaaatt
     6361 ctaaaattga gatttccaga aatgtagttt ctgatacttt aaaaaacgaa ttttcgcctc
     6421 aacgcatcac cgtattatct gcctcaatta atgacaaact ggaaatagat gaaaaaagta
     6481 aatctttctt gttagagccc tttgatcaaa tttttgtacg caaaactccg ggatttaact
     6541 ctcaggaaag tcttgtaatt aatggtgaag taatgtatcc gggcatttat ccaattttag
     6601 ataaaaatga aaaagtgttg gatctgatta aacgcaccgg aggattaact ccttatgcct
     6661 acctaaatgg tgctacttta gtacgtcttg ataataatca acaagaaatc atttttgaat
     6721 taaaaaatgc attcaaagac agcacctctc gtgccaatct tgtgttaaaa aacggagatg
     6781 tcattaatat tccaaccgtc aaccaacaag taagcattaa aggaaatggt attcgttatc
     6841 ctgatttaga ttccaaccaa accataagcg gtaaatttgt gccgggcaaa agtgccagat
     6901 ggtacataaa aaattatgcc gggggcttta aaaaaggagc aaataaacgc tctacttatg
     6961 tgatgtatcc aaataaaaag atggactata ctcgttttgc tatgggtata aaaaattatc
     7021 ctacagtgga ctatgaaggt gcagtaataa acgtggacat gaaagtgaaa aaaccaaaac
     7081 caccaaaagg tccggagtct aatataaatt ggaatatatt gttaccaagt atcattgccg
     7141 gagtaacgtc cttaggcaca acacttacac tcattttcgt acttaaaaac taagtagatg
     7201 caggaggaat atttttatga agaggacata aggctggttg atatacttga gtcttttggt
     7261 aattattttc gatatctgtt caagaaatgg tatattttct tacccggtgt aattggattg
     7321 actctatgcg ggtacttctt tgccacatta tcagcaccca aatttactgc aaatgccagc
     7381 tttaatgcca ttgactccag gataagctct atggggggct taatgtctat gatggggata
     7441 tcattttccg gaggaacatc caatgatgta ttaaccggga tcttttcttc tcgtaatatt
     7501 ttctataatt ccttattgca tgatatgatt acaaatgagg gtacccgaga gaaaatagga
     7561 aatgtatata tgcatacgct aaagtatgat gaaggttttg cagaagatcc caatcttaaa
     7621 ggttttaaat ttaaagctaa cgagataagc gagatgacta aattggaact gcagattgcc
     7681 agcatgatgt atgccgattt tgtggatgga tctttagatg ctgagtatga tattgctacc
     7741 ggattaatta aagcagaagt agaatctccg gagtatgaac tgtcaaagaa cttggcatcc
     7801 aatatgctca aagcaactat cgacttttat caacaaaagc aagtggagaa tgctaaagcc
     7861 tcttataaaa acactgacag acgtttagat tctatcaata atgagatatt aattcgccaa
     7921 cgaagaattg cacagaatca ggatattaac atatttaata aaaagcagat aagtgaaatt
     7981 gaggtacaaa aattatcaca agaattagca gtattaaatg tcatgtataa cgatgctacc
     8041 aactcaaaag aatctgcaaa agccggacta acgcctacca acatggtacg catcgtagac
     8101 gatcccatct tctcagtaga tgagattcat aaaggcaaaa tactatggtc tgctataggt
     8161 tttgcagcta gtttaatact tgtcgttatt cctcttttat tacataaagc cgttatagat
     8221 gggagagaag aaaataaagt acgtgaaact cgtatcgcca aaatgcagac ggaatagcag
     8281 gttttaagtt tttttcatta acttaccatt catatatggg agatgattta aatactaacg
     8341 atttactcca aattgacagc atcgctattg tgggtacagc gtgtcgtctt ccgggagatg
     8401 tttctcatat tgaagatctg tggcaacttg ttattaataa aaaggatggc ataactgaca
     8461 taccggaaaa cagaattccc aattttctat ccaatataga tgcaaaaaga ggtgcaggta
     8521 aaattgtaac cgctaaaggc ggattcttaa aggatatcga atactttgat gctgatttct
     8581 ttgccatatc cccaaaagaa tcagaaaaac ttgatcccca acaacggcta ttactcgaaa
     8641 cctcttttaa tgccatagaa gatgccggaa tttctttaga agaactaaat ggttctaaca
     8701 caggagtatt tattgcttca tggacaaacg actttgaaca ccgacttcag caaaccggaa
     8761 ctgatgttga tgtttatgca actacaggaa caggaagata cgcactgtct ggcagattat
     8821 ctttttttta caatttgcaa ggtccttctt taactgtaga tactgcttgt tcctcttcct
     8881 tagtagcaac acacctggct atgcaaagtc tgaagtctgg tgaatgcgat atcgcaattg
     8941 tcggagctgc aaatgtaatc ttagatacat tcgtttcggt tggttattcc agatcaggat
     9001 tattgtctga ttatggtggt tgttattttg gcaataaaaa cccaggcgga tatgtacgaa
     9061 gcgagggatc tgctgttatt attttaaaac gtacgaatga tgcattacgt gatggaaata
     9121 atatttatgc cctgttaaga gccagttctt gcaataatga tggacaagct ggcagacata
     9181 tgctttctcc cggtactgcc actcaagaaa tattaatgaa ttcagcctgc aagcatgcag
     9241 gaattaatcc ctcagctatt caatatattg aagctcacgg aaccggaaca aaagcaggtg
     9301 atattgctga aataaatgca atatggagaa gtttcggtca aaacaggaat cagaaactat
     9361 atataggttc tgtaaagtca aatattggac atacagaggc agtggctggt tttgccggac
     9421 tgtttaaact tttgatggca ttaaagtata aaaccatccc tccggatatt catctttatg
     9481 aaaaaaatcc gaaaataaat tgggaagcta tgaatataga aattcctctg gaagccttag
     9541 cctggcatca ggattctaat tccccaataa ttactggagt aaattctttt ggtgttaccg
     9601 gtactaatgc ttttgttctg gtacaggaat atgagaatat tgatactaaa caaacgaata
     9661 accctttttc aaccagaata cttccgctcc ccatctcagc aaattcaaac aaatcatttt
     9721 attcatatat aaaatcatat gacgaaatca taaacgaatc aaactggaag aacgtaatca
     9781 gtaatattgt ttaccataag gctaatctaa aatacagaaa ggtaatatta tataataa
//
LOCUS       DAOQII010000076         9639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3746_length_9639_cov_5.521390, whole genome shotgun sequence.
ACCESSION   DAOQII010000076 DAOQII010000000
VERSION     DAOQII010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3746_length_9639_cov_5.521390"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLF48_05180"
     CDS             <1..360
                     /locus_tag="PLF48_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF48752.1"
                     /translation="ETPFIIGDIPFIPIRAMHKTLPVLGFRIHDFTYITDANFIADTE
                     LEKIKGSKVLVLNALRREAHYSHFSLSQAIEIAQKINAEQTCLTHISHHMGLHQEVNK
                     ELPPGIKLAYDGLRIEL"
     gene            423..821
                     /locus_tag="PLF48_05185"
     CDS             423..821
                     /locus_tag="PLF48_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48753.1"
                     /translation="MEIHIHPALKLKEIQDVFHKRFPFLMLKFYSEKHKIGEGSHIED
                     LLPTDKQLSEFSSSDFSWHITGLMTAGELENTFQEKTGIGVQVFHKSVEVWLQTINTD
                     SKTLNELNAKGKEMNEVPLEGEPEDYHEHE"
     gene            complement(824..1687)
                     /locus_tag="PLF48_05190"
     CDS             complement(824..1687)
                     /locus_tag="PLF48_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48754.1"
                     /translation="MKIVRNKKNISYSSSSAMIHFRSQFFLIGDDAPEILCLDADWKE
                     IKRIKILDYPAVRIPKPIKPDLEAALVWNDKLYLLGSGSKLPQRSFIFEIDLENDKVS
                     QLDNLLNYERLNSDKRITELNMESALMINHKLLLINKGNISNSNSLIWTQPEFLLGDD
                     NVDFDFIPIVLPDTLIGYGIADATYIAEDDVLLLTATFENTTNAYDDGEIGGSVFIRI
                     DEAQKELAASVLRIENYIDLNRVSPEFNQQKIEAVCLCQKTKSHYNLFLAADNDDGLS
                     HLFEIQIKRNS"
     gene            1699..3369
                     /locus_tag="PLF48_05195"
     CDS             1699..3369
                     /locus_tag="PLF48_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48755.1"
                     /translation="MLFSRLYNQVFSNEESGTYSRLNVRTLIYLAIIILLGIKQLNGF
                     EHYLDISFFDESEYIKKGVLLHKHVYNDWGPSYNLWYYLLNLFTQDATKVFYLNYIIL
                     MISLPCLLFLLLVRFNIRQELALLFSLSLLMNGLLLSNFTYVSHFCTCIIFFGFLIST
                     YTKKNEIKAVVLFTSFYICMYARPEFLLTVISIFIIWLIILFKQKRITANPVYLATFS
                     VIFGLYLIFGFLSFNAQGIDRSYFAFMQHFITNYIVWHRNTVALIDFKHLDIFHGATT
                     MFQCMTHNPVMFFKHVGTNMLNYVLTLNLIIEKLLFPSSIFHFLGKFKHVLYGFFIIY
                     FCYLIIKKKGLLPIKSFFVNHSFTSLLILATFIWSYFSVFFIFPEKHYIIIQISWWIL
                     IICLVINDKTKWLSKSYIFIPILIIIGVLMPDAKEVGFFDYSVADNHKQPNLKTIHYL
                     IKNNTHKPVHLLTTETGFEAYLPDNYKEKFTPDKDIEPYIHGKEVDLYKFLNDYKINA
                     VFMNEKMIKVLGTDAGAQGDSLLLHPETAGFYKQKIDSSLKTYLLLQE"
     gene            3366..4562
                     /locus_tag="PLF48_05200"
     CDS             3366..4562
                     /locus_tag="PLF48_05200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF48756.1"
                     /translation="MTKNTNNANLNSRHYFPNLIGLRFWMALTVIYRHIEEVKYMRHI
                     QHDKLSIVKFHSVGYYPMLMFFTLSGFLISYQLEVEKKNTGNIVIRKFYKNRALRILP
                     LYYLSIFVYWVLMPNSPIQDYYNTIFFKPWSSGITATYYIPHWVYFFLSVVLLPHLAN
                     VILLVNERAWMYGVQHWSVGVEEIFYIFWPIFWKRITSFKKFIVRSYLLYLGLMIGVF
                     LAHLIFKKLLHNELVNFTSFVLFSFLSFSNLSCFFIGAVGIYIYLYRNDLTDRFVNKT
                     TFLIALFIILFFMFSSFEFPFFINEIVCSCYMLVVLYLLKTNKRYLIFEHPLFVYLGK
                     ITYAVYLVHFAVIVIVMNLLERFNLQHSNLLLFNILQYAGTLILTFSISALLYEYYEK
                     KFLAMR"
     gene            complement(4563..6236)
                     /locus_tag="PLF48_05205"
     CDS             complement(4563..6236)
                     /locus_tag="PLF48_05205"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNF48757.1"
                     /translation="MKIALAQLNYMIGDFQGNYAKMKAAVDYAVSQEADIILFSELCV
                     CGYPPRDFLEFKHFIHEAENIVQQLADFAGSRIAVVVGAPSRNTNKDGKDLFNSAYFL
                     QDGKIKFLAHKALLPTYDVFDEYRYFEPNKEFNIVEFKGKKIALTICEDIWDTGEENP
                     LYRINPVAELAKFKPDILFNLSASPFNYNQAKKRMDVVYKNATLFNLPVFYCNCVGAQ
                     TELIFDGGSIVMNAKGELIDELPYFEEAVRCYYLSDDNEQIAKIDSNCMVSVVQEQDK
                     NPIKLIHDALICGIRDYFKKLGFKQAILGLSGGVDSALTLVLAVRALGKENVLSVLMP
                     SQYSTSHSVDDSIKLLENLGSPYKIIPIKEVFDVYDSVLHPHFDGKPFDVTEENLQAR
                     IRGNYLMALSNKFGYILLNTTNKSEAAVGYGTLYGDMCGGIAVLADVYKTQIYEVCRY
                     INKNGEIIPEHILTKAPSAELRPGQKDSDSLPDYSVLDKILYQYIENRQGPDEIEALG
                     FEEDLVSRVLKLTNRAEFKRHQTPPILRVSSKAFGSGRRLPIVGKYLES"
     gene            6319..7362
                     /locus_tag="PLF48_05210"
     CDS             6319..7362
                     /locus_tag="PLF48_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48758.1"
                     /translation="MRNLLLIFSIISLLITSCTCNRNPEQQKQPKKSVEKQKEDIHVH
                     IIRFDRELFAANPNDIENEINKYKAKYPLFFPVYYNNVLSLPSAGDKSKQMDVMREFV
                     SSASMRGLYDSVQHTFPDLKFLEDDLNILFTNYTAYFPKKIIPHLFTCISEFSYSVFT
                     AGDSMLAFSLDKYLGARYLYYPAVFPEITYLYPTFDKKYIAIDGAQALASNIVPPPSD
                     RSTLLDKMLAEGKILYLMQAFLPDKKPNDIIKYSDKQWKWCNDNEQQIWSYFIEKKLL
                     YDTRFEQFKYVKDGPYTYGMPKESPGKVGAWVGWKIVQAFMREHPDYTVQQLLDEKDG
                     QKMLTESMYKPKV"
     gene            7369..7812
                     /locus_tag="PLF48_05215"
     CDS             7369..7812
                     /locus_tag="PLF48_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNF48759.1"
                     /translation="MLFQYINRNPEICRGSPVINGRRLTICNIILSCATSLESCKKDY
                     ELSLDTIKEAIIFCKDLHCFSQSDSSRTLNFCCNCILRNYTEPDEFEVGVFYDNLNKA
                     NSLEHKNELIHDYIGYRTWKIADLLFKHIFIENNHETNFNIAEID"
     gene            7882..8418
                     /locus_tag="PLF48_05220"
     CDS             7882..8418
                     /locus_tag="PLF48_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNF48760.1"
                     /translation="MLYLYVKSLHIIFVVTWFAAIFYMPRLFIYTTEAHEKTEPERSI
                     LIRQLLLMQNRLWTIIAWPSAILTLIFGVWMGILYGVLANWLIIKIGFVFMLYLYFLF
                     CHKVYLQQKKHIFTYTSMQLRVWNEVSTVLLFAIVFLVVVKTGMSWLSGIIGLLVLMI
                     LLMLGIKLYKLLRERNEN"
     gene            8595..>9639
                     /locus_tag="PLF48_05225"
     CDS             8595..>9639
                     /locus_tag="PLF48_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNF48761.1"
                     /translation="MKFATKVIHAGIEPDPSSGAIMTPIFQTSTYVQESPGKHKGYEY
                     ARTQNPTRDVLQNQLAALENGNHGICFASGLAATDAILKLFQSGDHIISSNDLYGGTY
                     RLFNRIYGKFGFQFSFVDMTDVANIEAAIRPETKLIWIETPTNPMLNIVDIEVVCALA
                     KKHQILVCVDNTFASPYLQTPLDLGADFVLHSATKYLGGHSDVIHGAIVVKDKTLADG
                     LYFLQNASGAVPGPQDCFLVLRGIKTLHLRMDRACENADKIANFLRNHPKVGNVNYPG
                     FEDHKGYAIAKKQMKLFGAMVSFSLKDDSFEAADKVLSNTKLFSLAESLGGVESLIGH
                     PATMTHASIPIEER"
BASE COUNT         3009 a         1805 c         1628 g         3197 t
ORIGIN      
        1 gaaacgcctt ttataatagg cgatattcca tttattccca ttcgtgctat gcacaaaacc
       61 ttaccagtat tggggttcag aattcatgat tttacctaca ttacagatgc caattttatc
      121 gcagatactg agctggaaaa aataaaggga agtaaagttt tagtactcaa tgcacttagg
      181 agagaagcac attattctca cttcagcctg agccaggcta ttgagattgc acaaaaaata
      241 aatgctgaac aaacctgtct tacgcatatc tctcaccata tgggattaca tcaggaagta
      301 aataaagaac taccacccgg tataaaatta gcatatgacg ggctccggat tgaactgtga
      361 tctgctgata aatatcatat ggttattagc aaaaaacctg tatttttata aaaaattgat
      421 atatggaaat acatatccat cctgctttga agctgaaaga aatacaagat gtatttcata
      481 aaagatttcc tttcctaatg ttgaagtttt actcagaaaa acataaaatt ggtgaaggtt
      541 ctcatataga agatttgttg cctacagata aacaactttc tgaattcagt tccagtgatt
      601 tttcatggca tataacaggc ttgatgacag ccggagagct tgagaatacc tttcaggaaa
      661 aaacaggaat tggtgttcag gtattccata aatcagtaga ggtatggttg cagactatta
      721 atacagacag taaaacacta aatgagctga atgcaaaagg aaaagaaatg aatgaagtgc
      781 ctttagaggg agaaccggaa gactatcacg aacacgaata gtttcagcta ttgcgcttta
      841 tctggatttc aaacaagtgc gatagaccat catcattatc cgctgccaga aacaaattgt
      901 aatgtgattt tgttttctga cataaacaaa cagcctcaat tttttgctga ttaaattcag
      961 gcgaaaccct atttaggtcg atatagtttt caattctcag caccgatgct gccaattctt
     1021 tttgagcctc atctatacgg atgaatacgc tacctcctat ttcaccatca tcataagcat
     1081 tagtcgtatt ctcgaaagta gctgtaagca gtaacacatc atcttcagca atataagttg
     1141 catcagcaat accatatcct attaaagtat caggcagcac gatggggatg aagtcaaagt
     1201 caacattgtc atcacccaag agaaattcgg gctgtgtcca tatcaatgaa ttcgagtttg
     1261 aaatattgcc tttattaatc aagagaagct tatgattaat catcaaagca ctttccatgt
     1321 ttaattcggt gatgcgttta tctgaattta atctttcata attcaataag ttatctaact
     1381 gactaacctt gtcattttcc aaatcaattt cgaaaataaa actgcgttgc ggtaatttag
     1441 aaccggaacc caacagatac agtttatcat tccaaaccag tgctgcttct agatccggct
     1501 taattggctt tggaattctg acagcaggat aatcaagtat tttgattcgt tttatttcct
     1561 tccaatctgc atccaaacaa agtatctcag gagcatcatc accgattaag aaaaactgag
     1621 aacgaaaatg aatcatcgct gagcttgacg aatagctgat gtttttttta ttccgaacta
     1681 ttttcataca tttaaaatat gcttttctca agactttata atcaagtatt ctctaatgaa
     1741 gaatcaggaa cttattcccg tctaaatgta cgaactctta tttacttagc tataatcatt
     1801 ctgcttggca ttaagcaatt aaatggtttt gagcactact tagatatttc attcttcgat
     1861 gagtcggaat atataaagaa aggagttctc cttcataaac atgtttataa cgattggggt
     1921 ccttcttaca atttgtggta ttacttactt aatcttttca cacaagatgc aacaaaagtt
     1981 ttttatctca actatatcat actaatgatt agtctgccct gtttgttatt tttattattg
     2041 gttcgcttca acatcagaca agaacttgca ttattgttta gtctttcatt acttatgaat
     2101 ggtcttttac tgagtaactt cacctatgtc agtcattttt gtacttgtat catctttttt
     2161 ggctttttaa taagcacata tactaaaaag aatgaaatta aagcggtcgt actatttacc
     2221 tcattttata tttgtatgta tgccagacct gaatttttac ttaccgtaat ttctatattt
     2281 ataatctggc taattatact ttttaagcaa aaaagaatta cggctaaccc tgtctattta
     2341 gcaacattct cggttatctt cggtttatat ctgattttcg gcttcttatc tttcaatgca
     2401 caaggcatag accggagtta ttttgcattt atgcagcatt ttatcaccaa ttatattgtt
     2461 tggcatagaa atacggtagc gttgatagac ttcaaacatc tcgacatctt tcatggtgct
     2521 acaaccatgt ttcaatgcat gacgcataat ccggttatgt ttttcaaaca cgtcggcacc
     2581 aatatgctca actatgtctt aaccttaaat ctgattatag aaaaactctt gtttcccagc
     2641 tcaatatttc attttttagg caaatttaaa catgtcttat atggtttttt tataatttat
     2701 ttttgctatc tgatcattaa aaagaaggga ctattaccaa taaaaagttt ttttgtaaat
     2761 catagcttta cttcactgct tatactggca acttttatct ggagttattt ttctgtcttt
     2821 tttatatttc ctgaaaagca ttatattatc attcaaatca gctggtggat actcattatc
     2881 tgtctggtaa taaatgacaa aacaaaatgg ctaagcaaaa gctatatttt tattccaata
     2941 ttaattatca taggggtttt gatgcccgat gcaaaagagg ttggtttctt tgattattct
     3001 gttgctgata atcacaagca accgaacctt aaaacaatac attatttaat taaaaataat
     3061 acacataagc ccgttcatct gttaactact gagaccggat ttgaagccta tttgcctgac
     3121 aattataaag agaaatttac gccagacaaa gacatagaac cttatataca cggcaaagaa
     3181 gttgacctct ataaatttct taatgactat aaaattaatg ctgtttttat gaatgaaaag
     3241 atgataaaag ttcttggaac agatgcaggt gcccaggggg atagcctttt gctgcatccg
     3301 gaaaccgctg gcttttacaa acagaaaatt gacagctcat taaaaacgta tctcctactt
     3361 caagaatgac gaagaataca aataatgcaa atttaaacag ccgtcattat ttccctaact
     3421 tgattggctt aagattctgg atggcactta ctgtcattta cagacatatt gaagaagtaa
     3481 aatatatgcg tcatatccag catgataagc taagcattgt caaatttcat tctgtaggct
     3541 attatcctat gcttatgttt tttaccttaa gtggatttct tatttcttac caactggaag
     3601 tagagaagaa aaataccggg aatattgtta tccgcaaatt ttataaaaac agagcattgc
     3661 gaattcttcc cctttactac ttatccatct ttgtttactg ggtattaatg ccaaactctc
     3721 ccattcaaga ctattacaat actatctttt ttaaaccttg gtcttcagga attactgcta
     3781 cctattatat ccctcattgg gtgtattttt tcctctctgt tgttttgttg cctcacttag
     3841 ctaatgttat tctgttagta aatgaacgag cttggatgta tggcgtgcaa cactggtcgg
     3901 tgggtgtaga ggagattttt tatattttct ggcctatttt ctggaaacgc attacatctt
     3961 tcaaaaaatt tattgtaaga agttacctgc tttaccttgg gcttatgatt ggtgtttttt
     4021 tagcccatct cattttcaag aaattattac acaatgaact ggtcaacttc acatcgtttg
     4081 tcttattttc atttttatca ttttctaatc tttcgtgctt ttttattggt gcagttggta
     4141 tctacatcta cttatacaga aatgatttga cagatcgatt tgttaataaa actacttttt
     4201 taattgcttt atttatcatt ttgtttttca tgttcagcag tttcgaattc ccatttttta
     4261 taaatgagat tgtctgttct tgttatatgc ttgtagttct ttatttgtta aagacaaaca
     4321 aacgctatct tatttttgaa caccccttat ttgtttatct gggtaaaatt acttatgccg
     4381 tttatctggt acattttgca gtaatcgtga ttgttatgaa tctattggaa cgttttaatt
     4441 tacaacattc taatttatta ctatttaata tccttcagta tgccggaacg cttatcctta
     4501 cgttttcgat aagtgcatta ctgtatgaat attatgagaa gaaatttctg gctatgcgct
     4561 aatcagctct ctaaatattt tcctactatc ggcaatcttc tgccacttcc aaatgctttt
     4621 gacgaaacac gtaaaatggg tggtgtttga tgtcgtttaa actctgcacg attcgtcaat
     4681 tttaagacac gagaaactaa atcctcttca aagcccaggg cttctatctc gtcaggtcct
     4741 tgtctgttct caatatactg atataagatt ttgtccagca cactgtaatc cggaagcgaa
     4801 tcggaatctt tttgtcccgg cctgagctca gcacttgggg ctttagttaa aatatgttca
     4861 ggtataattt ctccgttttt attgatatat cggcacactt cataaatttg tgttttgtaa
     4921 acatccgcca aaacagcaat accaccacac atatcgccat ataacgtacc atagcctacg
     4981 gcggcctcgc ttttattggt tgtatttaaa agaatatatc caaacttatt gctcagtgcc
     5041 attagataat ttccccttat gcgtgcctgc aaattttctt cagttacatc gaatggctta
     5101 ccgtcaaaat gcggatgcaa aacggaatca tatacatcaa aaacctcttt gataggaata
     5161 attttatatg gagagcctaa attttcaagc aacttaatac tatcgtctac ggaatgtgag
     5221 gtagaatatt gcgaaggcat caacacgctc aacacattct cttttcccaa tgctcgcacg
     5281 gctaacacga gagttaatgc agaatctact ccaccgctca atcccaaaat ggcttgctta
     5341 aatcccagct ttttgaaata atctcgtatg ccgcaaatga gcgcatcatg tatgagttta
     5401 atcggatttt tatcttgctc ctgtactacc gaaaccatac agttgctgtc aatcttggct
     5461 atctgttcat tatcgtctga caaataataa cagcgtactg cttcttcaaa atacggcaat
     5521 tcatcaatca actcaccttt tgcattcatc acaatagagc caccgtcaaa aatcaattcg
     5581 gtttgtgcac cgacacaatt gcaataaaac acgggcagat taaatagtgt ggcattttta
     5641 tacacgacat ccattctctt tttagcctga ttataattga atggcgaagc agaaagatta
     5701 aataaaatat ccggcttaaa tttagccagc tctgccactg gatttatgcg gtacagcggg
     5761 ttttcttcac ccgtatccca gatatcttcg cagatagtca gagcaatttt tttgccttta
     5821 aattcaacaa tattaaattc tttattcggt tcaaaatagc ggtattcatc aaatacatca
     5881 taggtaggca atagcgcttt atgtgctaaa aacttgattt tcccatcttg caaaaaatag
     5941 gcagaattaa acaaatcctt gccgtcctta tttgtgttgc gagatggtgc acctaccact
     6001 acggcaatcc ggcttcccgc aaaatctgct aattgctgta caatattttc tgcttcgtga
     6061 ataaaatgct taaattctaa aaaatctctg ggcggatagc cacatacaca gagttcgctg
     6121 aaaagaatga tatcggcttc ttgtgaaacg gcataatcta cggcagcttt cattttggca
     6181 tagttgcctt ggaaatcgcc tatcatgtaa ttgagctggg ctaatgcaat tttcataatc
     6241 taaaataaag taaatgtcct aataaaaatt aattctcgca aaaatcggat ttcaattttt
     6301 attttagcag tatattttat gagaaattta cttttaatat tcagtattat aagcttgctt
     6361 atcacttcat gtacttgcaa ccgcaatcca gaacaacaaa aacaacctaa aaagagcgtg
     6421 gaaaagcaaa aagaggatat tcatgttcac attatccgct ttgacagaga attgtttgcc
     6481 gccaatccca acgatatcga aaatgaaatc aacaagtata aagctaaata tcctttgttt
     6541 tttcctgtat attataacaa tgtactcagc ctgccctctg ccggtgacaa aagcaagcaa
     6601 atggatgtca tgcgcgagtt tgtttcatca gccagtatgc gcggattgta cgacagtgta
     6661 cagcatactt tcccggattt gaagtttctg gaagatgact taaatatctt attcacgaat
     6721 tacaccgctt attttccaaa aaaaatcata cctcatcttt ttacctgtat atctgagttt
     6781 tcttattccg tatttacagc cggcgacagt atgcttgcct tttctttaga taaatacttg
     6841 ggtgcaaggt atctctatta tccggcagta tttcctgaaa ttacctatct ctatcccact
     6901 ttcgacaaaa aatatatagc catagatggc gctcaggcac tggcttccaa tatcgtacct
     6961 ccaccctccg accgctctac actcttggat aagatgctgg ctgaaggaaa aatcctgtat
     7021 ttgatgcagg cttttttgcc ggataaaaaa ccaaatgaca ttatcaaata ttctgataaa
     7081 caatggaaat ggtgcaatga caatgaacag caaatctggt cgtattttat cgaaaagaaa
     7141 ttgttgtatg atacccggtt tgaacaattc aagtatgtaa aagacggccc atatacttac
     7201 ggtatgccga aagaaagtcc cggaaaagtg ggagcatggg tgggttggaa aattgtacag
     7261 gcttttatgc gcgagcatcc agattatacc gttcagcaat tattagatga aaaagacggt
     7321 caaaaaatgc tgacagagtc gatgtataaa ccgaaagtat aaaatagcat gttatttcaa
     7381 tatattaatc gcaaccctga aatttgtcga ggctctccag taattaatgg aagaagactt
     7441 actatttgca acataattct aagctgtgcg acatcattag agtcttgcaa aaaagattat
     7501 gaattatcat tagatactat taaagaagca atcatattct gcaaagattt acactgtttt
     7561 tcacaatctg acagctctag aacattaaat ttttgctgta attgtatctt acgaaattat
     7621 acagaaccag atgaatttga ggttggggta ttttatgaca acctaaataa ggctaatagc
     7681 ttagaacata aaaatgagct aatccatgat tacattggat atagaacatg gaaaatagca
     7741 gatttgctgt ttaagcatat atttatagaa aacaaccatg aaactaattt taatattgct
     7801 gaaatagatt agtttttata tcacatttca ttataaaatc aaaatcaacc tacgaggttg
     7861 aaaataactt atttttgcag gatgctctac ttgtatgtaa aatctttgca catcatcttt
     7921 gtagtcactt ggtttgctgc tatcttttat atgccccgtt tgtttattta cacgacagaa
     7981 gcacacgaaa aaactgaacc agaacgaagc atcttaattc gccagctttt attgatgcaa
     8041 aaccgtcttt ggacaattat agcttggcca tctgccattc ttacactcat tttcggtgtc
     8101 tggatgggca ttttatatgg tgtgttagcc aactggctca taataaaaat cggatttgtg
     8161 tttatgcttt atctctattt tttattttgc cacaaagttt atcttcaaca aaaaaagcac
     8221 attttcactt atacatctat gcaattgcgt gtttggaatg aagtttcaac ggtcttgctt
     8281 tttgccatcg tgtttttggt tgtagttaaa acgggtatga gttggctgag tggtataatt
     8341 ggattacttg ttttgatgat attgcttatg cttggcatca aactttataa gctattgaga
     8401 gaaagaaatg aaaactgacg ctattttagc ttattttact atatttacaa catgaaattt
     8461 gtatctactt actgttgtat caccataaac cgaaaataaa gcggaacaga ttatactata
     8521 aaaaaccgct taatgttagg cggttttttt attttcgtca accaaaaaca aactcaacat
     8581 ctcaaattat acaaatgaaa ttcgctacca aagttatcca tgccggcata gagcccgatc
     8641 cgtcatccgg tgcaatcatg acacccattt tccagacttc tacctatgta caagaatcac
     8701 cgggcaaaca taaaggttac gagtatgcgc gcacacaaaa cccgacacgt gatgtattac
     8761 agaatcagtt agccgccctt gaaaatggca atcatggcat ctgttttgcg agtggtctgg
     8821 cagctaccga tgcgatactc aaactctttc aatccggcga tcatatcatc tcttccaacg
     8881 atctgtatgg tggcacttac cgcctattca accggattta tggaaaattc gggtttcagt
     8941 tttcatttgt tgacatgaca gatgtagcta atatcgaagc agcaatacgt cctgaaacaa
     9001 aattaatctg gatagaaact cccactaacc cgatgctgaa tatcgtggat atagaggtcg
     9061 tgtgtgcctt agccaaaaaa caccaaatct tagtttgtgt agataatacc tttgcgtcac
     9121 catatctaca aacaccgtta gatttgggtg ctgatttcgt actgcattct gcaactaaat
     9181 atttaggcgg acacagtgat gtgatacatg gggctatcgt agtaaaagat aaaacactgg
     9241 cggatggctt gtatttttta caaaacgcaa gtggtgccgt accgggaccg caagattgtt
     9301 ttttggtatt gcgtggcata aaaactttgc atttgcgtat ggatcgggct tgtgaaaatg
     9361 ctgacaagat tgcaaatttc ttgcgcaatc atcctaaggt gggcaacgtt aattatccgg
     9421 gatttgaaga ccataaaggg tatgcgatag caaaaaaaca aatgaaatta tttggcgcta
     9481 tggtttcctt ttctttgaaa gacgacagtt ttgaagctgc ggataaagta ctttccaaca
     9541 cgaaattatt ttcattggct gaatcattag gcggagtaga gagtttaata gggcatcctg
     9601 ccacgatgac ccatgcaagt attccgatag aagagcgcc
//
LOCUS       DAOQII010000077         9559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3789_length_9559_cov_5.170770, whole genome shotgun sequence.
ACCESSION   DAOQII010000077 DAOQII010000000
VERSION     DAOQII010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3789_length_9559_cov_5.170770"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2757
                     /locus_tag="PLF48_05230"
     CDS             <1..2757
                     /locus_tag="PLF48_05230"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNF48762.1"
                     /translation="LDAFLPHYWNFSLSQSYYTNSLNGFTSDCFSAKSSNLYTSKKWN
                     PSSPWAFHNYDGLNLFGLHLGNYYGYYGRGGGKMYMSKASAPMMLESANFAVDAAPAH
                     NGGDVFEKKDEETVGTYNYTTTAATDSTLIAPDKPKPQVTPRTNLKETAFFFPQLQTD
                     SEGNVIINFEMPEALTRWKFMGLAHTKDLNSTVFEKEVVTQKELMVVPNAPRFLREGD
                     KFSFSAKITNLSASALSGMVALTIKDALTGKEINTAFKNLNATKMFAVAAGANTPVSW
                     DIEVPQCVSAITYEVVAQAGNFSDGEGAPLIVLPNRMMVTETLPLWVSGKSKKSYKFD
                     KLLNNKSATLKNYSLTLEMTSQPVWYAVQALPYMIEYPYECAEQLFSRYYANTLASHI
                     ANANPDFKKIYAKWSSTNSDALLSNLEKNQELKTVLLEETPWVRDAQNETEQKKRIAL
                     LFDLNKMATEQQAALKKLRDMQTSNGGFTWFPGMPDNRFITQHIVAGIGHLEKLGVLN
                     RKESKDVNDMTDKAVTYLNARLKEELENIKKWDKDYLKNNHLDYSILHTLYALSFFSP
                     EKSEAYTYFIEQGKKYWLDRDLYSKAMLALVLQRNSEKATADKIITALKQNSIENEEL
                     GMYWKANESGGWHWYQAPVETQALLIEAFHEVARDEKSVDEMKVWLLKQKQTTAWKST
                     KATAEACYAILLQGTNWAKSDNLVEVSLNKIKVEPQKMDATIEPGTGYYKVKWSGDAI
                     KPEMGKIEIKKTDKGPAWGAMYWQYFEDLDKITSAETNLKLNKKLFKKVQTASGVQLQ
                     EITPQTPLNPGDLVTVRIELRTDRNMEYVHLKDMRAAGFEPVNVLSQYKWQDGLGYYE
                     STKDVATHFFMDWMNKGIYVFEYDVRATLAGNFSNGITSIECMYAPEFTSHSKGERVT
                     IVKK"
     gene            2860..3834
                     /locus_tag="PLF48_05235"
     CDS             2860..3834
                     /locus_tag="PLF48_05235"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HNF48763.1"
                     /translation="MLNFLHSKQISTIAFLLLLFAVLKIPFALYSFPDIQSLSTLWGL
                     SGAFFSNTPLLSWLIAQICILLQAFWFNYLFTEADYYERFSFVPALYYILVTSLLPVF
                     NLFSIYTLVAFIFLALLHNALIINLRKSAKMEAFNMGLLGGILVLLDVHFLFFLPFLF
                     LMLYVLKPFRFKEFMLLLFGWMMPVYFYLAIAYLFDVHINLSAYSLKHFSFFRFDRDV
                     FGSINLIATALLILFSFISLRGIMYSTGFKRKKNVSMLIVLIIATTIILLFSGRHAPD
                     MLQIVFIPASVLLSLLMLRIRKKKLGEIFNAVYIALILAVNLLRIFYS"
     gene            3848..4549
                     /gene="purQ"
                     /locus_tag="PLF48_05240"
     CDS             3848..4549
                     /gene="purQ"
                     /locus_tag="PLF48_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNF48764.1"
                     /translation="MKFGVVVFPGSNCDKDVIHVCSEVMQCETRILWHKDTSLQDLTT
                     DDCIMLPGGFSYGDYLRCGAIARFSPIMEKIIEHANNGGYVWGICNGFQILCESHLLP
                     GALLANNHQRFICQNQYLLPQTQNSVITRYADLSEPLNIPIAHGEGRYYANAEMMQQL
                     KENDQILFRYCDEKGNINEAGNPNGSLDNIAGICNAQRNVFGMMPHPERAAEDVLGNS
                     DGKVLFESLLNLVKA"
     gene            4609..6642
                     /locus_tag="PLF48_05245"
     CDS             4609..6642
                     /locus_tag="PLF48_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNF48765.1"
                     /translation="MNRLYYFISLFLIGCTTSAPKIAQAQIEQHLFWKSEVVKTAENE
                     FDVKINCTLDEGWHVYSQFTEEGGPLPTKFTFEKNKDIELIGKVKEVGKLEKKFDDLF
                     GVNVSSFANKVTFIQHVKVKSPNAVLKGSFDGQVCQEACILFGPENFEIKFDNNQKVS
                     LPSISKSIDSANGSSSAIPNPEINTTIKKAVQLSGKFDTTYANNSCSVRAEAKDKSYW
                     WIFLAGFIGGLLALLTPCVFPMVPLTVSFFTKRGKDQKEGLKKAIIYGISIIVIYVLL
                     GLIITTVFGSDALNAMSTNIWFNLLFFVVFVVFAISFFGAFEITLPSSWANKTDALSS
                     KGGNLGIFFMAFTLALVSFSCTGPIIGTLLVEAATGGGPTILGRIPLKPLIGMFGFSF
                     ALALPFTLFALFPQWLQSLPKSGGWMGTMKVVLGFLELALAFKFLSTVDMTMNWGVLR
                     FEPFMIIWILIFAAMTLYAFGIFKKKENKEKRSTGVTLLGIVSLAITLYFSYALINYK
                     AIPLLSGIIPPTSYNFKNTNKENFVHFKDYEQGLAYAKAHDMPVLLDFTGFGCVNCRK
                     VEDAVWTDKYVIDVMKRYVVISLYVDDKTALPESEWYTSSATGKERQVKEVGQKWSDF
                     QAKHFGTNSQPYYILLNNKEQMLNQPVAFNFAIKPQNYISFLQCGLKMQEHLK"
     gene            6691..7758
                     /locus_tag="PLF48_05250"
     CDS             6691..7758
                     /locus_tag="PLF48_05250"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HNF48766.1"
                     /translation="MSKELRHEKNCLNCGHFVPDRFCSHCGQENVETKESFLHLVKHV
                     FEDITHFDSKFLNTVKTLFLKPGQLTLEYLHGKRVKYVPPVRLFIFTSFVFFVFIGIL
                     KHKPDGHKKQKTLEHYIEQVQDSVLLDSSEQDDFLNGFKEGMQTVHNNTDKQDEDTDI
                     LRTGLINIKLNKKDTLTGAKQEFEDIGSDAEAYLRKQDSLPEEKKDGYFSRKLKLFIL
                     ENKHKTSDEFKEEFNEKFYHKMHYWIFILFPFFALVMKLYYLKNNMYYSEYLIFGFHF
                     HTIILLVLLLITIIHYYTDYSAYGWGLLAVVLYLFFSLKNIFNQSVGKTLLKTFLISF
                     TYMVIVNIFLLISSVFVIFFS"
     gene            7766..8911
                     /locus_tag="PLF48_05255"
     CDS             7766..8911
                     /locus_tag="PLF48_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNF48767.1"
                     /translation="MKKTVALILCFIVIPVAYFYFSNAGRIKSIDQIYPRYVIGDVEN
                     IGTNKGKGNIIALSPYLHTYDFSSAKAFENMLRYYLNFARQKKLLSDSTVVVLPEYFG
                     TWLVALNEKASVYSDTSITDAMQTIVYSNLFSFAKAYLVSTGKDREKEAIFRMKAQKM
                     AYVYQQTLSKLSKEYKLTIVAGSIVLPEPEIENGILKIHPNGKIYNVSVVYDMSGKAR
                     SPFIKKVFPINSELGFTSKANNPETPVFITPLGKIAVLICADSWFPQSYTSLNHKDIK
                     AVVSPAYVNTTDVWNAKWKGYDYGTPPDVTQADKNNLTEVEAWKKYAVVARAKPAGIP
                     YALNVFLRGDFWNLGSDGNTLLLKQDSLIEAKDQVEKTGSLINLWLE"
     gene            complement(8925..>9559)
                     /locus_tag="PLF48_05260"
     CDS             complement(8925..>9559)
                     /locus_tag="PLF48_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48768.1"
                     /translation="RAWVECDIKDLGNVGGLEPEVFAEKFRTAVRIAEVDPHRATTHN
                     KGIYNGVDAMIIATGNDFRAVEACGHTYAARNGSYSSLTHCSLENGKFKFWIDLPLAV
                     GTVGGLTQLHPLVKISMHVLGNPNATELMQFCAVAGLAQNFAALRALTTVGIQQGHMK
                     MHLLNILNYYKATEEEKKYALEYFKDKIVSFTTTRVLLEQIRGVKIDMDE"
BASE COUNT         2936 a         1813 c         1886 g         2924 t
ORIGIN      
        1 ttagatgcct ttttacccca ttactggaat tttagtttat cgcaatcgta ttataccaac
       61 tccttaaatg gatttacatc cgattgtttt tctgccaaaa gcagcaattt atatacttcc
      121 aaaaaatgga atccttcctc tccatgggca ttccacaatt acgatggatt gaatttattt
      181 ggattgcact taggcaatta ttacggctac tatggaagag gtggtggaaa aatgtatatg
      241 tctaaagcat cagcaccaat gatgctggaa tctgctaact ttgctgtgga tgctgctcct
      301 gcacacaatg gaggtgatgt atttgaaaag aaagatgaag aaacagttgg cacttataat
      361 tatacaacaa cagctgcaac tgattcaact cttattgcac ctgataaacc caaacctcaa
      421 gttacaccgc gcaccaatct gaaagaaact gctttcttct tcccgcaatt acaaacagac
      481 agcgagggca atgtcatcat caattttgaa atgccggaag cactgacaag atggaaattt
      541 atgggtctgg cacataccaa agatttaaac agcaccgttt ttgaaaaaga agtagttacc
      601 caaaaagaac tgatggtcgt gcccaatgcg ccgcgtttct tacgcgaagg cgataagttt
      661 tctttttcag ctaagatcac caatctttct gcatctgctt taagcggcat ggtggcatta
      721 accatcaaag atgcattaac cgggaaagaa atcaataccg catttaaaaa tctgaatgca
      781 acaaaaatgt ttgctgttgc tgccggagcg aatactccag tttcatggga tatcgaagtg
      841 ccacaatgcg tgagtgccat cacctacgag gtggtagcac aagccggcaa tttcagcgat
      901 ggcgaaggtg caccgctgat agtattgccc aaccgcatga tggtaacaga aactttgcct
      961 ttgtgggtga gcggcaaatc caagaaatcc tacaagtttg ataaattact caacaataag
     1021 tctgcgacat taaaaaatta cagccttacc ttagagatga cttctcaacc ggtgtggtac
     1081 gccgtacagg cattgccgta tatgatagaa tatccttacg aatgtgccga gcagttattc
     1141 tctcgttatt atgccaacac cttagcatcg cacatcgcaa atgccaaccc ggatttcaaa
     1201 aagatttatg cgaaatggag tagcacaaat tcggatgcgc tgttgtctaa cctcgaaaaa
     1261 aatcaggaat taaaaactgt cttactcgaa gaaacacctt gggttcgcga cgcgcaaaat
     1321 gaaacagaac agaaaaaacg catcgcctta ttattcgact taaataaaat ggctacggag
     1381 caacaagccg ccctaaaaaa actgcgtgat atgcagacat ctaacggtgg ctttacttgg
     1441 tttccgggca tgcccgacaa tcgctttatc acacagcaca tcgtagccgg aattgggcat
     1501 ctggaaaaat taggcgtact caaccgaaaa gaaagcaagg atgtaaacga catgaccgat
     1561 aaagcagtta catatctcaa tgcccgactg aaagaagaat tggaaaatat taaaaaatgg
     1621 gataaagatt atttgaaaaa caatcatctc gactattcca ttttgcatac gttgtacgca
     1681 ctttctttct tctctcctga aaaatctgaa gcttatactt attttattga acaaggtaaa
     1741 aaatactggc tcgaccgcga tttgtattcc aaagcaatgc tggctttggt tttgcaacgc
     1801 aatagtgaaa aagcgactgc tgataaaatt attaccgcat taaaacaaaa ctccatagaa
     1861 aatgaagagt tgggcatgta ctggaaagcg aatgaaagcg gcggctggca ttggtatcag
     1921 gcaccggtag aaacacaagc tttgttgata gaagccttcc acgaagtggc aagagacgag
     1981 aaaagtgtgg acgaaatgaa agtgtggtta ttaaaacaaa aacaaacaac ggcatggaaa
     2041 tccaccaaag ctacggctga agcttgttat gctatattgt tacaaggaac gaactgggcg
     2101 aagtcggata atctggtgga ggtaagttta aacaaaataa aagtagaacc gcaaaaaatg
     2161 gatgctacca tagagccggg cacaggctat tataaagtaa aatggagcgg cgatgctatt
     2221 aaaccggaaa tgggaaaaat agaaatcaag aagacggata aaggtccggc atggggcgct
     2281 atgtattggc agtatttcga agatttggat aaaatcactt cagccgagac caacttgaaa
     2341 ctgaataaaa aactgttcaa aaaagtacag acagcttcag gtgtgcaatt gcaggagatt
     2401 acgccacaaa cacctttgaa tccgggcgat ttggttactg tacgcatcga attgcgcacc
     2461 gaccgcaaca tggaatatgt acatttgaaa gacatgcgtg ccgccggatt tgagcctgtt
     2521 aatgtattgt cgcaatacaa atggcaagac ggcttgggct attacgaaag cacgaaagat
     2581 gtggctacgc atttcttcat ggattggatg aataagggaa tttatgtatt tgaatatgac
     2641 gtgcgagcta cgctggctgg taacttcagc aacggcatca cctcgataga gtgtatgtat
     2701 gcacccgaat ttacttcaca ttccaaagga gaaagagtta caatagtaaa aaaatagcag
     2761 aacagttgtg ccacgattat cgtggcacaa ctgatagtct taacgtacaa aacacaagtg
     2821 aatttgttcg ccaaggcata aaaagtgtat ttttgctgca tgctgaattt tttacattcc
     2881 aaacagattt ccactattgc atttttgctg ttgctgtttg ctgtacttaa aattccattt
     2941 gcattatatt cctttcctga tattcagtcg ctgagtactt tatggggatt aagtggtgca
     3001 ttcttcagca acacccctct cctttcatgg ctgattgcac aaatatgcat cttattgcag
     3061 gcattttggt ttaattacct ttttaccgaa gccgactact acgaacgatt ttcgtttgtt
     3121 ccggccttat attatatatt ggttacatcg ctgttgccgg tatttaatct ctttagtatt
     3181 tacacacttg ttgcgtttat atttctagcc ttattacaca atgcacttat tatcaatttg
     3241 cgtaagagtg caaaaatgga agcctttaat atgggtctgc tgggtggcat tttagtctta
     3301 ctagatgttc attttctgtt ttttctgcct tttttatttt taatgctcta tgtattaaag
     3361 ccattccggt ttaaagagtt tatgttgctt ttattcgggt ggatgatgcc ggtttatttc
     3421 tatcttgcca tcgcttattt atttgatgtg catatcaatc tgagtgcata ttcgctcaag
     3481 catttttcat tttttcgttt tgaccgggat gtatttggca gtattaatct tattgccacc
     3541 gcattattaa ttctgttttc ctttatcagc cttcgcggca ttatgtattc caccggattt
     3601 aaacgcaaaa aaaacgtgag tatgcttatc gtactcatca ttgccacgac cataatttta
     3661 ttattcagtg gtcggcatgc accggatatg ctacaaattg tatttattcc ggcttcagta
     3721 ttattatcgt tgctgatgtt gcgcatccgc aaaaagaaat tgggcgaaat ttttaatgca
     3781 gtatatatcg cgctgatttt agccgtaaat ttgttgcgta ttttttattc ttaaacagta
     3841 gtctaaaatg aaatttggag tagttgtatt tcccggttct aattgtgata aagatgtcat
     3901 ccatgtatgc agcgaagtga tgcagtgcga aacacgcatc ctttggcata aagataccag
     3961 ccttcaggat ttaactacag atgattgcat catgctgccg ggaggatttt cttatggcga
     4021 ttatttgcgt tgtggtgcta ttgcccgttt ttcacccatc atggaaaaaa tcatagagca
     4081 cgcaaataat ggaggttatg tatggggtat ttgcaatggt ttccagattt tgtgcgaatc
     4141 gcatttattg cctggggcat tgttggcaaa taatcaccag cgttttattt gccaaaatca
     4201 atacttatta ccacaaactc aaaatagtgt cattactcgc tatgccgatt taagcgagcc
     4261 tttgaatata ccgatagcac acggtgaagg tcgttattat gccaatgcgg aaatgatgca
     4321 gcaattgaaa gaaaacgacc agattttgtt ccgctattgt gatgaaaaag gcaatatcaa
     4381 tgaagccggc aacccgaatg gaagtttaga taacatagca ggtatttgta atgcccaaag
     4441 aaatgtattt ggtatgatgc cccatccgga aagagcggct gaagatgttt tgggaaattc
     4501 ggatggaaaa gtcctttttg aatcgctgtt aaatttggtt aaagcctgat ttatccggtt
     4561 ttctttgcga ttttacttgt ttatttttat agtttcattt tattactcat gaatagactt
     4621 tactatttta tatcattatt tctaattggt tgcacaacta gtgctcctaa gattgctcaa
     4681 gcccaaatag aacaacattt attttggaag agtgaggtgg tgaagactgc tgaaaacgaa
     4741 tttgacgtaa agataaattg cacacttgat gaaggctggc atgtttactc tcagtttacc
     4801 gaagaaggcg gacccttgcc cacgaaattt acctttgaaa aaaataagga tattgaatta
     4861 attggcaagg ttaaagaagt cgggaaatta gaaaaaaaat tcgacgattt gtttggtgtg
     4921 aatgtgtctt cttttgccaa taaagtaact tttatacaac acgtaaaagt gaaatcgcca
     4981 aatgccgttt taaagggctc ttttgacgga caagtttgcc aggaagcctg tatcttattt
     5041 ggcccggaga actttgaaat caaattcgac aataatcaga aagttagttt gccgtccatt
     5101 tcaaaatcga ttgattccgc taatggtagc tcatcagcaa tacccaaccc tgaaatcaat
     5161 actaccataa aaaaagcagt ccagctatcc gggaaattcg ataccacgta tgccaacaac
     5221 tcatgcagtg tacgtgcaga agccaaagac aaatcctact ggtggatttt tcttgccggc
     5281 ttcatcggcg ggttattagc cctgcttaca ccttgtgttt tccctatggt accactcacg
     5341 gtaagttttt ttaccaagcg cggtaaagac caaaaagaag gcttaaaaaa ggccatcatt
     5401 tacggtattt ccattattgt catttatgtt ttattgggtt tgattataac tactgttttt
     5461 ggctctgatg ccctcaatgc catgtccacg aatatatggt tcaatctcct gttttttgta
     5521 gtgttcgtgg tcttcgccat ttcatttttc ggggcatttg aaattacact tccctcatct
     5581 tgggcaaaca aaacagatgc cctttcgtca aaaggaggaa atctgggcat tttctttatg
     5641 gcatttacac tggctttagt ttccttttcc tgcacgggtc ctattatcgg tactttatta
     5701 gtagaagcag ccacgggcgg tggtcctacg attttaggca gaattccatt aaagccacta
     5761 attggcatgt ttggattttc atttgcttta gccttaccct ttacgctgtt tgctttgttt
     5821 ccgcaatggc tgcaatcatt gcccaaatcc ggaggctgga tgggtacgat gaaagtggta
     5881 ttaggctttc tggagttggc actggcattc aaatttttat caacggtaga tatgactatg
     5941 aactggggtg ttctgcgttt tgaaccgttc atgattatat ggattttgat ttttgctgcc
     6001 atgacacttt atgcattcgg cattttcaag aaaaaagaaa acaaagaaaa aaggtcgaca
     6061 ggcgtaactc tcttaggaat agtatcttta gcaataacgc tctatttttc gtatgcatta
     6121 atcaactaca aagctattcc attgctgagt ggtattattc cgccaacgtc ttataatttc
     6181 aaaaatacaa acaaagaaaa cttcgttcat ttcaaagact atgagcaagg tttggcgtat
     6241 gccaaagcgc acgatatgcc tgttttatta gattttacgg gctttggttg tgtaaactgc
     6301 cgcaaggtgg aagatgcagt ttggacggat aaatatgtga ttgacgtgat gaaaagatac
     6361 gtggtaattt cactttatgt tgacgataaa acggcattac cagaaagtga atggtacact
     6421 tcatcagcta ccggaaaaga aagacaagta aaagaagtag gacagaaatg gagtgatttt
     6481 caagcgaagc attttggcac gaattcccag ccgtattata ttttattaaa taacaaagag
     6541 caaatgctca accagccggt agcttttaat tttgccatca aaccgcaaaa ttatatttca
     6601 ttcttgcaat gtggattgaa gatgcaagaa catttgaaat agacatttct ttgtgttcac
     6661 gcatatgatt gcgtacattt gcatttctcc atgtcaaaag aattgcgtca tgaaaagaac
     6721 tgcctgaact gcggacattt tgtacccgac agattttgtt cgcattgcgg tcaggaaaat
     6781 gtagaaacga aagagtcgtt cttgcattta gtaaaacacg tttttgaaga catcacgcat
     6841 tttgattcta agttcctgaa tacggtaaaa acgttatttc taaaacccgg gcaactaact
     6901 ttagaatact tacatggcaa gcgagttaaa tatgttccgc ctgttcgatt atttatcttt
     6961 acctcttttg ttttttttgt ttttatcgga attctcaaac ataaaccgga tgggcataag
     7021 aaacaaaaaa cgcttgagca ttatattgaa caagttcaag actcagttct gctagactct
     7081 tccgaacaag atgattttct taacggtttc aaagaaggca tgcaaacggt gcataacaat
     7141 acggacaagc aggatgagga cacagacata ctgagaacag ggctcataaa tattaaacta
     7201 aacaagaaag atacgcttac aggtgcaaag caagaatttg aagatattgg cagcgatgcg
     7261 gaagcttatc taagaaaaca ggacagcctg ccggaagaaa agaaagatgg atatttctct
     7321 cgtaaattga agttatttat acttgaaaac aaacacaaga ctagtgatga attcaaagaa
     7381 gaatttaacg agaaatttta tcataaaatg cactactgga tttttatatt gtttcccttc
     7441 tttgctctgg taatgaagtt gtattatctt aaaaacaaca tgtactattc agaatatttg
     7501 atattcgggt ttcattttca cacgataata ctacttgtac tcctgttaat taccatcatt
     7561 cattattata cagattattc cgcttatgga tgggggctgc tagctgtagt attgtattta
     7621 tttttctcat taaaaaatat atttaatcag tctgtcggaa aaacgctgtt aaaaaccttc
     7681 ttaatttcat ttacctacat ggttatagtc aatatttttc ttctaatttc atctgtattt
     7741 gttattttct tcagctaaag attctatgaa aaaaacagtt gccttgattc tatgctttat
     7801 tgttatcccg gtagcttatt tttatttttc aaacgcaggc agaataaaat caatagatca
     7861 aatctatcca aggtatgtta ttggcgatgt cgaaaacatt ggaacaaaca agggcaaagg
     7921 aaatattatt gcactttcgc cctacctgca tacgtatgat ttctcatctg cgaaggcttt
     7981 tgaaaacatg cttcgatatt atctcaactt cgcgcgacaa aagaaactac tcagcgattc
     8041 taccgtggta gttttgcctg aatattttgg tacatggctg gtagcactca atgaaaaagc
     8101 ttctgtttat tcagatactt ccattacaga tgccatgcaa acaatcgtgt attccaattt
     8161 attttctttt gcaaaagcat atctcgtatc tacagggaaa gacagggaaa aagaagctat
     8221 tttcaggatg aaagcacaaa aaatggcata tgtttatcaa cagacattat ccaaattatc
     8281 taaagaatac aagctcacca ttgttgctgg ttctatcgta ttgcctgagc ctgaaattga
     8341 aaatggcatt ttgaaaatac atcccaatgg taaaatttac aacgtaagtg ttgtttatga
     8401 tatgtcggga aaagcgcgtt ctcccttcat aaagaaagta tttcccatca atagcgaatt
     8461 gggttttacc agtaaagcga acaatcccga aacgccagta tttataacac ctttgggcaa
     8521 aattgcagtt ttgatttgtg ctgattcgtg gtttcctcaa tcttatacat cattaaatca
     8581 taaagatata aaagcagttg tttccccggc ttatgtaaac actacagatg tctggaatgc
     8641 caaatggaaa ggctatgatt acggcacacc accggatgta acacaggcag acaaaaataa
     8701 tcttactgaa gttgaagctt ggaaaaagta tgctgtagtg gcgagggcaa aacctgccgg
     8761 gattccatat gcactaaacg tatttctgcg cggtgatttc tggaatttgg gtagcgatgg
     8821 caataccctc ctgcttaagc aagactcttt aatagaagca aaagaccaag ttgaaaaaac
     8881 aggtagctta atcaacctat ggttagaata ataccgcaag aattttattc atccatatca
     8941 atttttacac ctctgatttg ttccagtaaa acacgggtag tagtaaatga aacaattttg
     9001 tctttaaaat attccagtgc atatttcttt tcttcttctg ttgctttata ataattgaga
     9061 atgtttagca aatgcatttt catgtgtcct tgctggattc ccacagtagt tagcgcacgc
     9121 aatgctgcaa aattttgtgc caaccctgct actgcgcaaa attgcatcag ttctgtagcg
     9181 ttgggattgc ctagtacatg catactgatt ttcactagcg gatgtaattg tgtaagtccg
     9241 ccaacggtac caacagccaa aggcaaatct atccagaatt taaattttcc attttcgagt
     9301 gaacagtgtg tcaagctcga atagcttcca ttgcgtgcgg cataggtatg tccgcatgct
     9361 tctaccgcac gaaaatcgtt gcccgttgca attatcatgg catccacgcc attgtaaatt
     9421 cctttattgt gtgtagtagc cctatgagga tctacttctg caattcttac tgccgttctg
     9481 aatttttctg caaatacttc cggctctaaa ccaccaacat ttcccaaatc tttgatatca
     9541 cattccaccc aggctctag
//
LOCUS       DAOQII010000078         9548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3795_length_9548_cov_5.778784, whole genome shotgun sequence.
ACCESSION   DAOQII010000078 DAOQII010000000
VERSION     DAOQII010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_9548_cov_5.778784"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /gene="ftcD"
                     /locus_tag="PLF48_05265"
     CDS             <1..746
                     /gene="ftcD"
                     /locus_tag="PLF48_05265"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNF48769.1"
                     /translation="IDMRVQNGSHPRMGACDVFPIIPLQNCTIQDAVLLSQTLGKKIG
                     DKLDIPVYLYEQSASIASRKNLADLRKGEYESIAGKIYLPEWKPDFGKPIFNEKFGMM
                     ALGARDFLIAYNINLNTQEEHIAKTIAAKIRSIRNTDLPFPFQFVKAIGWYQEAFLCA
                     QVSMNITQIYESKILDVFRAVQKLAEDDGVSVNGSELIGLIPLQALYYPNMSVSEVIN
                     ELGLNSVKPFSPDENILEIKLGLSDGHPA"
     gene            730..1635
                     /locus_tag="PLF48_05270"
     CDS             730..1635
                     /locus_tag="PLF48_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNF48770.1"
                     /translation="MDILHKPVSLLLFFLLGISWQFAFPDNRFIYKKINRFILYISLP
                     AIVLSKIPFIALNKTVLIPVGAAWLLFLGCIIFAYLMKRFLGYSNKTFACIVLCCGLG
                     NTSFVGYPVLTYLYGAESLQYAIFVDQPGSFLIMSTLGIFMATYFNSGKLNWTKISKL
                     LLTFPPFLVFIFSLFVPAKLLQAPILQVLNFIGGWMVPLALLSLGMQFQFKISHIPIK
                     PFFTGIIYKLILGPLLIYILIFFVLKKQGMMAEVSVLECAMPPMITSSILAGNFGLDE
                     ELAAVFPTMGILISIITLSIWTLIL"
     gene            complement(1668..2435)
                     /locus_tag="PLF48_05275"
     CDS             complement(1668..2435)
                     /locus_tag="PLF48_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF48771.1"
                     /translation="MSYFDKIKQYLSGELSAEDKYTFELEMENDPFLKEAVDGFELLH
                     DDNHSFPLSEASITDGVIINKAVGKSVPLQTNDTIARKSSSRLLAMAAIVVGIALVLW
                     FLTQSNKQQTLNTDAIYAGYYKPLTHPDALVRGENNNASPAVEAYEKEDYFEAVNIYR
                     KLVEADSNNVKNKLFLGISYMSTNQPQKAIATLSTLLESQEYYHDIRWYLALAYLKTK
                     QIDHAEKLFSQLAKEDNFYQENAKEISDKLGSSLAQN"
     gene            complement(2468..3028)
                     /locus_tag="PLF48_05280"
     CDS             complement(2468..3028)
                     /locus_tag="PLF48_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF48772.1"
                     /translation="MTNHLDKLSDAELLRRYKVDADMDAMGLLYKRYSVLVYGLSYKY
                     LRHEESAKDAVNEIFLLILEKSVNYDVTFFKTWLYTLSKNYLMRVVQKDKSAVFTDFE
                     NNLEKFMENEEDETLYIQDVKEASLREAISNLNDEQRICIEQFYLNKKSYSEVAEITG
                     LDLNKVKSSIQNGKRNLKLYLDKTKR"
     gene            3097..3783
                     /locus_tag="PLF48_05285"
     CDS             3097..3783
                     /locus_tag="PLF48_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48773.1"
                     /translation="MHKILLIMALCSFSGCHSNTKPSHQSEQPTNNTSEKISYTSLKE
                     KIQLEKEMIKKSKPTSSKIADKYIVPNLLQLFPYWMGTEWNFYGTTETPKQGTIACGY
                     FVTTLLRDAGLKIPRVKLAQQASSQIIAAVCDKQDIHSFSLTQTNNDSFFDFIRKQGK
                     ALYIIGLDFHTGFIYYDGDALRFIQAKYYGEKCVVDEDATQSAVILQSKFKQIGKISG
                     SEKSEKLFLK"
     gene            complement(4443..4526)
                     /locus_tag="PLF48_05290"
     tRNA            complement(4443..4526)
                     /locus_tag="PLF48_05290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4490..4492),aa:Leu,seq:cag)
     gene            4688..5956
                     /locus_tag="PLF48_05295"
     CDS             4688..5956
                     /locus_tag="PLF48_05295"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNF48774.1"
                     /translation="MLSILYFHSIMAHVSINGYAHPAFEKVKQAFEDNFHKRNDIGAT
                     CCIYFKGEKVVDLWGGKKHNGEHWNENDVSIVFSTSKGISSLAFALLHSKGYLDYDEK
                     VSTYWKEFAYNGKEEITIRQLLAHQAGLFSNDKSLTYEVLTNPDILSNALAKQKTYWK
                     PGLYRGYHAWTIAMYQNEICKRVDPQNRTLNQFAAEELFVPLEADFSIGMKPDFDFSR
                     YAHIIPFYPHEALKEKGHTDIREILKGILNPFSFFYKSLLNPPHALNLNNFNKRKYLA
                     LPLGSAHGIGNARSIAAIYNEFATGGKKLGINKDTLAQLEASPIPCKSNKYDLVLHAD
                     IPFSLGFAKPSFFQNFGVNYRAYGTFGAGGSGGFADPEKEIAFSYVMNKMGTHIANDP
                     RELALRTAVYDCVFELEKNKQETLKLKTTV"
     gene            5953..6402
                     /locus_tag="PLF48_05300"
     CDS             5953..6402
                     /locus_tag="PLF48_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF48775.1"
                     /translation="MNSNTEAAERLQYLCTTILDKLSSIPLTEFTHKPNPDKWSKIEI
                     LGHLIDSAANNHQRFVRLQFEQEPVIYYEQNEWVKYNYYVQQVPAELIALWKHYNTFL
                     ARLIAKIPEEKMHAVCILRHGESVQLSFLISDYIRHLEYHLEQILAS"
     gene            6462..7316
                     /locus_tag="PLF48_05305"
     CDS             6462..7316
                     /locus_tag="PLF48_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF48776.1"
                     /translation="MSTIKEANVLVTGGANGIGKLMSLKCLQEGAGNLILWDINEENL
                     ENTKKEFGKKGYNNVHTYVVDVSQVKDIEKAATEVLLEVGNVDILINNAGIVAGKQKF
                     WEYSVRDIEKTLSINVNGVMHVARVFIPEMIKQGRGHVINIASASSLIPLPKGSVYAS
                     SKWAVMGWSESLRLELEAEGQNLHVSTICPSYIDTGMFKGVKAPMLFPLLQPEEVTTN
                     IIKAIKTNKPVLMMPENVNLVPFLKGVFPTRIFDTVAGWLGVYNSMSSFEGRKEEERI
                     PEKKTKVK"
     gene            7814..8719
                     /locus_tag="PLF48_05310"
     CDS             7814..8719
                     /locus_tag="PLF48_05310"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNF48777.1"
                     /translation="MKKQKVRFIVNPKSGGTFGSRFTERMVKRNLDLNRFSYDMYYTR
                     YAGHAIKLAQQSLIDKVDIIAVVGGDGTQNEVGQVIMDKNVLMANIPTGSGNANARTL
                     GIPLHIPDAIQLINTGKPFPIDALMINGQASFMASGIGYDGLVIDRFQKLPLRGVASY
                     MASILSTALTYELPDFTIQIDDQAPFSRKAFTVLITSMGAVGFGIKFNHISKPNDGIF
                     EITVINEFNKVRIPEIIRESFFGDVASADFMETFQVHKKLKLSLNKVESLQMDGDFHG
                     RTDSIEVTLKKQALRYIVPQDFDIN"
     gene            8724..9065
                     /locus_tag="PLF48_05315"
     CDS             8724..9065
                     /locus_tag="PLF48_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNF48778.1"
                     /translation="MSTNDWKKRLGTVYSTNPDFNYETENDNEQDTLENAKQLLIVFT
                     DNKKRKGKIVTLVTGFVGKLEDLEMLAKMLKTKCGVGGSVKDGEIIIQGDFKQKIKEL
                     LEKEGYKVKLR"
     gene            9062..>9548
                     /locus_tag="PLF48_05320"
     CDS             9062..>9548
                     /locus_tag="PLF48_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNF48779.1"
                     /translation="MITAHEISGLKQSVAQHKKQYSRMINQLKSMTSYDADELIQTQH
                     DQVFSEINCLDCANCCKTTPALLIHEDINRISKYLRITVKDFLHNYAVRDEDGDIVFK
                     QTPCVFLGEDSRCSIYNVRPKACREYPHTHQKNQQKILNITEKNLDICPAVKRIFDAL
                     LS"
BASE COUNT         3108 a         1697 c         1792 g         2951 t
ORIGIN      
        1 taattgatat gcgtgtgcaa aatggcagcc atccgcgcat gggggcttgt gatgtttttc
       61 ctattatccc gctgcaaaac tgcacgatac aggatgctgt tttactcagt caaacattag
      121 gcaaaaaaat cggtgataag ttagatatcc ctgtttatct ctatgagcaa tcggcgagca
      181 tagcatcacg gaaaaatttg gccgatttgc gcaaaggtga atatgaaagc atcgccggaa
      241 aaatctattt acccgaatgg aaacccgact ttggcaaacc gattttcaat gaaaaattcg
      301 gcatgatggc attgggtgca cgcgattttt taattgcata taatatcaac ctgaatactc
      361 aagaggaaca tatagcaaaa acaatagctg caaaaatcag aagcattcgt aatactgacc
      421 taccatttcc gtttcagttt gtaaaagcca taggctggta tcaagaggcg tttctgtgtg
      481 cacaggtttc tatgaatatc acacagattt atgaaagcaa gattttggat gttttcaggg
      541 ctgttcaaaa attggcagaa gatgacgggg tgagcgtaaa cggcagtgaa cttattggat
      601 taattccttt gcaggctctt tattatccta acatgtcggt ttcggaagtg ataaatgaat
      661 tgggattaaa tagcgtaaaa cctttctcgc ccgacgaaaa tattttagaa attaaacttg
      721 gactaagcga tggacatcct gcataaaccg gtcagtcttt tactgttttt tttgctgggc
      781 ataagttggc aatttgcatt cccggataat cgttttattt ataaaaagat taatcgtttt
      841 atcttataca tctctcttcc ggcaattgtg cttagtaaaa tcccatttat tgcattaaac
      901 aaaacggtgc ttatccctgt cggtgctgcc tggctattgt ttctaggttg cattattttt
      961 gcctacctca tgaaacgatt cttgggttat agcaacaaaa cttttgcatg tattgtattg
     1021 tgctgtggct tgggcaacac ctcctttgtt ggctatcccg ttttaactta tctgtatggc
     1081 gctgagagcc tgcaatatgc catttttgtt gaccaacccg gcagttttct gataatgtca
     1141 acgcttggta tttttatggc aacatacttc aacagcggaa aattgaactg gacaaaaatc
     1201 agtaagctat tactcacctt tccgccgttt ttagtcttta ttttcagctt gtttgttccc
     1261 gcaaaactcc tgcaagctcc tattttgcaa gtgctcaatt ttattggggg atggatggtg
     1321 cctttggcac tcttatccct gggtatgcaa tttcagttta aaatcagtca tattcctatt
     1381 aaaccatttt ttacaggcat tatttataaa ctgattttag gtcctttgct tatttacatc
     1441 ctaatttttt ttgtattgaa aaaacaaggc atgatggctg aagtgagtgt cctggaatgc
     1501 gcaatgcctc ccatgattac ttcttcgata ctggcaggca attttggatt agatgaagaa
     1561 cttgctgctg tgtttccgac tatgggcatt ttgatttcta taatcacttt aagtatttgg
     1621 acactgattt tgtaaacaac tacaaatgtc gaaacaaaat gaataaacta attctgtgcc
     1681 agcgaactgc ctaacttatc tgaaatttct ttggcatttt cctgataaaa attatcttct
     1741 ttagccaatt gactgaataa cttttccgca tggtcaattt gtttagtttt taaatacgct
     1801 aaagccaaat accagcggat atcgtgataa tattcctgcg attctaataa agtactcagc
     1861 gtggctatag ctttttgtgg ctgatttgta ctcatatatg aaatacccag aaataattta
     1921 ttttttacat tattagaatc cgcctccacc agcttgcggt agatattgac agcttcaaaa
     1981 taatcttctt tttcatacgc ttctactgca ggagaggcgt tattattttc gccacgcacc
     2041 aatgcatccg gatgggtaag cggtttgtag tagccggcat aaatggcatc agtatttagt
     2101 gtttgttgtt tattgctttg ggttaaaaac cagagtacca gcgcaattcc cacaacaatt
     2161 gcagccatcg ctaacaagcg agatgatgat tttcgggcta ttgtatcatt ggtttgtaga
     2221 ggaacagatt ttccaacagc tttgttgatt ataacaccat cagttattga agcttccgat
     2281 agaggaaaac tatgattatc atcatgaagc aactcaaatc catcaacagc ttcttttaag
     2341 aaagggtcgt tctccatttc cagttcaaat gtatatttgt cctctgcaga aagttcaccg
     2401 gaaagatatt gttttatttt atcgaagtag ctcatgtttg cttgtatttg tattttcaat
     2461 taaagaatca gcgctttgtt ttatccaaat aaagtttcag attgcgtttt ccgttttgga
     2521 tactgctctt tactttattc aaatctaagc ctgtaatttc agcaacttcg ctataggatt
     2581 tcttattcag ataaaattgt tcaatacaaa tacgttgttc gtcatttaaa ttgctgatag
     2641 cctctcggag tgacgcttct ttcacatcct gtatatatag agtctcatct tcttcatttt
     2701 ccataaattt ttccaaatta ttttcaaaat cagtaaaaac tgccgattta tccttttgga
     2761 cgaccctcat caggtagttt tttgataagg tgtaaagcca ggtcttaaaa aaagtaacat
     2821 catagttgac cgatttctcc aaaatcagca gaaagatttc gttcaccgca tccttcgcac
     2881 tttcttcgtg tcgcaagtac ttgtacgaaa gaccataaac cagcacagaa taacgcttgt
     2941 aaagcaagcc catggcatcc atatctgcat ccactttata acggcgcaat aattcggcat
     3001 cagaaagttt gtctaagtga tttgtcataa atatgaaaac aatatacaca ataaaaatga
     3061 aatcccgcag tttactatac acattaaact atccgtatgc ataaaatatt gctgattatg
     3121 gcactttgct ccttctcggg atgtcactcc aacacaaagc cttcccatca atctgaacaa
     3181 ccaacaaata atacaagtga aaaaataagc tatacatcgc tgaaagaaaa aatacagcta
     3241 gaaaaagaaa tgataaaaaa gtcaaaaccc acaagctcta aaattgcaga caaatacata
     3301 gtgccgaatc tgctacagct atttccctat tggatgggaa cggaatggaa tttttacggc
     3361 actactgaaa cgccaaaaca aggaactata gcctgtggct atttcgttac aacactgctt
     3421 cgagatgccg gtttgaagat tcccagagta aaacttgcac agcaagcctc ttcgcagatt
     3481 attgctgccg tatgtgataa acaggatatt cattcctttt ctctcacaca aaccaataat
     3541 gattccttct ttgattttat acgcaaacaa ggaaaagctc tttacattat cggactggat
     3601 tttcacaccg gatttattta ttatgatgga gatgctttac gatttattca ggcaaaatat
     3661 tacggcgaaa aatgtgttgt agatgaagat gccacacagt cggcagttat cttacaatca
     3721 aagttcaaac aaataggaaa aataagcgga tccgaaaaat ccgaaaaatt gtttctgaaa
     3781 taattcctct gacactcaaa ggtgtcgcca ctacgtggct ttctgataag aataaaattt
     3841 gtaccactac taaactatta aactcattaa ctaatccacc aatgaaggtg tcgcctatac
     3901 gtggtttgtt tcttaataaa ttatttacca ttaaggcatt aaggaaatta agaatatttg
     3961 cggacaaaga ggctgcctaa gttcaagtgt taaatgcaat tttttgggtc ataatcaata
     4021 ctttcataat atccgtttga cttacagatt ttagctgaag gataagcatg aaaataagta
     4081 gaataaaagt ttggaattgc attctttact ttttcgactg cctcattttt tttgaaaaaa
     4141 tattcagcat acccaaaatt gtaatcagtt ttaccataat aaatagaagt gaacatattt
     4201 gaataaaagt gcattttaac ccatttgtcc aaattgtcaa actctatgaa atctcattct
     4261 ataactacag aaaaagggaa ttttttaatt agttctaatc tgctttctaa attatcatca
     4321 tctaatccta atgaatgagt cttgtaaact ttttccattt cattaaaagt aaatatcctc
     4381 atacgtgtaa atgaatgtta ttgatatgtg ctatggattt aaagtgataa aaaataaaat
     4441 tgtgcccaag acaggactcg aacctgcatg ctttttacgg cgccagaacc tgaatctggt
     4501 aagtctacca atttctccac ttgggcaata agtataagaa cattctttgc aaaaatacga
     4561 atttttacaa gagtaaaaaa cagatgtaaa atttggcatg tttataatct aataccttat
     4621 acacatatat tataagcaat cctaaagcta tcgtagtttt tgaattaatg acaggtaatt
     4681 tcattttgtg ctaagtattt tatactttca ttctattatg gcgcatgtaa gtattaacgg
     4741 ttatgctcat cctgcatttg aaaaagtgaa acaagctttt gaagataatt ttcataaacg
     4801 aaatgatatc ggagctactt gttgtattta ttttaaaggt gaaaaagtag tcgacttatg
     4861 gggtggtaaa aaacacaatg gagagcattg gaacgaaaat gatgtaagca tcgtgttttc
     4921 taccagcaag gggatttctt ctttggcttt tgcgttatta cattccaaag gatatttaga
     4981 ttatgatgaa aaggtcagca cttactggaa agagtttgct tataacggaa aagaagagat
     5041 cactattcgt cagttgcttg ctcatcaagc cggactattc agcaatgata aatcattgac
     5101 ctacgaggta ctgacgaatc ccgacatttt atctaatgca ttggcaaaac aaaaaacgta
     5161 ttggaaaccc ggtttgtatc gtggctatca tgcctggacg attgccatgt accaaaatga
     5221 aatttgcaag cgtgttgatc cgcaaaacag gacgcttaat caatttgctg cggaggaatt
     5281 atttgttccg cttgaagcag atttcagcat aggcatgaaa cccgattttg attttagcag
     5341 atatgcacat atcattccat tttacccgca cgaagcatta aaagagaagg gacataccga
     5401 catccgtgaa attttaaaag gcatacttaa tcccttttcc tttttttata aatcactttt
     5461 aaatccaccg cacgcactta atctcaataa ttttaataaa agaaaatact tggcgctgcc
     5521 cttgggttcg gcacacggca ttggcaatgc ccgcagtatc gctgcaatct ataatgagtt
     5581 tgctacagga ggtaaaaaat taggcatcaa taaagatacg ctggcacagt tagaggcctc
     5641 gccaattcct tgcaaaagca ataaatacga tttagtattg catgcggata ttcctttttc
     5701 attaggattt gccaagccat cattcttcca gaactttgga gttaattatc gtgcttacgg
     5761 cacttttggc gcaggtggct cgggtggttt tgccgacccg gaaaaagaaa ttgccttttc
     5821 gtatgtgatg aataaaatgg gcacgcatat tgctaacgac ccgagagaat tagcgttgcg
     5881 cacggctgtt tatgattgtg tttttgagct ggagaaaaat aaacaagaaa cactaaaact
     5941 aaaaacaacg gtatgaattc caatacagaa gctgcagaaa gattacaata tttatgtaca
     6001 acaattctgg ataaattaag cagtattcct ttaactgaat ttactcataa acccaatcct
     6061 gacaaatgga gtaaaataga aatacttggt catttgatag acagtgcagc aaataatcac
     6121 cagcgttttg tccgattgca atttgaacag gaacctgtca tttattatga gcaaaatgaa
     6181 tgggtgaagt ataattatta tgtccaacaa gtacccgcag agcttattgc tttatggaag
     6241 cattataata cttttttagc ccgtttgatt gcgaaaattc cggaagaaaa aatgcatgct
     6301 gtttgcattc ttcgccatgg agaatcggtt cagctttcat ttttaatttc ggattatata
     6361 cgccacttgg aataccatct tgaacaaatt ttggcatctt aacttaagtt aaaccaagct
     6421 gtactaaatt tcattacttt tataattaaa aataaatcag tatgtcaact ataaaagaag
     6481 ccaacgtatt agtaaccggt ggagctaacg gaatcggtaa attaatgagc ttaaaatgtc
     6541 tgcaggaagg tgcgggtaat cttattttat gggatattaa cgaggagaat ctcgaaaata
     6601 ctaaaaaaga attcggtaaa aaaggatata acaatgtaca cacttatgtt gttgatgtat
     6661 cgcaggtaaa agatattgaa aaagcggcta cggaagttct gctcgaagtg ggtaatgtag
     6721 atattttgat caacaatgcg ggcattgtag ccggaaaaca aaaattctgg gaatattcag
     6781 ttcgcgatat cgagaaaaca ctgagcatta atgtgaatgg agtgatgcat gtggctcgtg
     6841 tatttattcc ggaaatgata aaacaaggaa gaggacatgt catcaatatt gcatcagcat
     6901 cctcattaat accattgccg aaaggctctg tttatgcatc gagcaaatgg gcagtaatgg
     6961 gctggagcga aagcctgaga ttagagttgg aagccgaagg gcaaaatttg cacgtatcta
     7021 cgatttgccc gagttatatc gacacaggca tgtttaaagg cgtgaaagca cctatgttat
     7081 tcccattatt gcaacccgaa gaagtaacga ccaatattat aaaagcaata aaaacgaata
     7141 agcccgtttt gatgatgccg gagaacgtga atttagtgcc cttcttaaaa ggcgtatttc
     7201 ctacacgcat cttcgatacc gttgcaggat ggttaggcgt ttataattcg atgagcagtt
     7261 ttgaaggcag gaaagaagaa gagcgtattc ctgagaagaa aactaaggtg aagtaggaat
     7321 acatttagaa ttcttatttc tcaactttta ctttaaaaat tagattttat atctactggt
     7381 aaggtttgat taataattct taccacattg aggcataatt tttttaggat taacaattct
     7441 aaataataca agaattgaag taagagtaga aaaatatatg aatagccaaa ggcgatacta
     7501 tgtttttgct tgacagtcta tacaaatttg taccaaacaa ctatttatga ttaaagatgc
     7561 tatgtttcta tgtggtttaa actgcataac ggattctata ttatgcaatt acttcgtatt
     7621 acgaaatccc gaatttttgc agaacttcaa cattcaatat gatttggaaa ccacatagat
     7681 ttatgtttga aatgcaagaa aatatttgag aatagttttt tacaattaag ccttaatagg
     7741 cagcttaccg gctaggaatt atttatatcc aaaaaataca gcattcttct tacatttgca
     7801 aaaatatatt tgtttgaaga agcaaaaagt tcgttttatt gttaacccga aatcgggagg
     7861 tacattcggt tcgagattta ccgaacgcat ggtcaaaaga aatctggacc tcaaccgctt
     7921 ttcgtatgat atgtattata cccgttatgc aggtcatgcc atcaaattag cccagcaatc
     7981 gctgatagat aaagtggaca ttattgccgt ggtaggcgga gatggtacgc aaaatgaagt
     8041 aggacaagtc atcatggata aaaatgtgtt gatggcgaat atccctaccg gttcaggcaa
     8101 tgccaatgcc cggacgctag gcatacctct gcatataccg gatgccatcc agctgataaa
     8161 tacaggcaaa ccttttccta ttgacgcact catgatcaac ggacaagctt cttttatggc
     8221 cagcggtatt ggatatgatg gtttggtaat tgaccgtttc cagaagctgc cgttaagagg
     8281 tgtagcctct tatatggcta gtattttaag tactgcactc acctatgagt tgcccgattt
     8341 taccatacaa attgacgatc aagcgccttt ttcgagaaaa gcatttactg tactgattac
     8401 cagtatggga gctgttggtt ttggcattaa attcaatcat atcagcaaac ccaatgacgg
     8461 catttttgaa attacggtca ttaatgaatt taataaggtg cgcataccag agataatacg
     8521 ggagtcgttt tttggagatg tggcgagtgc tgattttatg gaaacttttc aagtacataa
     8581 aaaacttaaa ctatctttaa ataaagtaga aagtttgcaa atggatggcg actttcacgg
     8641 cagaacagac agtatagaag taacacttaa aaaacaagcc ttgcgttaca ttgtgccgca
     8701 agattttgac ataaactaat aacatgagta ccaacgactg gaaaaagaga ttaggaacgg
     8761 tgtattccac caatcccgat ttcaactatg agacggaaaa tgacaacgag caagatacac
     8821 ttgaaaatgc aaagcaattg ttgattgtat ttacggataa taaaaagcgc aaaggaaaaa
     8881 tagtaacctt ggtcacgggc tttgtaggca agctggaaga tttggaaatg ttggcaaaaa
     8941 tgctgaaaac aaaatgcggc gtgggtggtt ctgtcaaaga tggtgaaata attatacaag
     9001 gagattttaa acaaaaaata aaagaactgc tggaaaaaga aggatataaa gtaaagctga
     9061 gatgattaca gcgcatgaaa tttcaggttt aaaacagtct gttgctcaac ataaaaagca
     9121 atactcgcgc atgattaatc agcttaagtc tatgacatcc tatgatgcag atgaactgat
     9181 acaaacacag catgatcaag ttttttcgga aatcaattgt ctggattgtg ccaattgctg
     9241 taagactacg cctgccttat taattcatga agacatcaat cgtatcagta aatatttgcg
     9301 aattacagtc aaagattttc tgcataatta tgcagtcaga gatgaagacg gtgatatcgt
     9361 ttttaaacaa actccctgtg tgtttttagg agaagatagt aggtgttcca tatacaatgt
     9421 aagacctaaa gcctgtagag aatatccgca tacacatcaa aaaaaccaac aaaaaatatt
     9481 gaatatcacg gaaaaaaatc tggacatctg cccggcagta aaacgaattt ttgatgcttt
     9541 attgtctg
//
LOCUS       DAOQII010000079         9444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3847_length_9444_cov_5.293535, whole genome shotgun sequence.
ACCESSION   DAOQII010000079 DAOQII010000000
VERSION     DAOQII010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3847_length_9444_cov_5.293535"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1260)
                     /locus_tag="PLF48_05325"
     CDS             complement(88..1260)
                     /locus_tag="PLF48_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48780.1"
                     /translation="MTVTIIIIICVLLLLAYVFDISASKTKIPSVILLLLLGFLFKQI
                     TLYFQIEVPDLNFLLPILGTVGLILIVLEGSLELEYNKSKIPLIRKSMLVALLPMLIF
                     SAIFAALFSHFGNISFQAGLANAIPFAVVSSAIAIPSAQNLSDSNREFVTYESSLSDI
                     FGVIFFNFITLNEVITFGSFTKFTFEIMLMLVITVIATLGLAFLLSKIKHHVKYAPII
                     LIIILIYAITKIYHLPGLLFILLFGLSLGNLDVFKDVSFIKLLKPEILEEEVHKFREL
                     VIEMAFLVRALFFLLFGFLINLNELLNVDAVVWTMGILATIYILRFIFLTLFKINLNP
                     ILYIAPRGLITILLFLNIPERQSVNLVNNSLTIQVILLTAIVMMFGLMFQKKEITE"
     gene            complement(1318..4374)
                     /locus_tag="PLF48_05330"
     CDS             complement(1318..4374)
                     /locus_tag="PLF48_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNF48781.1"
                     /translation="MKQFFTLIFFFYFLSGLQAQSVMQEQKAFYDKYGYTKEADWDKL
                     QEQLTGVKFKKLHNDNTNQRLATCSLNGRRVYGWHPYWNGSTLHNNYKWNLISDLCYF
                     DYTINPSTGANSNTSFAWSTSSAVTAAKNNGVKISFCATLFSSHSTFLTSTTAKNTFT
                     QNCISLLRSRGGNGVNLDFEGVSSTQKANLTAFIQQFCTTMHDSVPGCEISIALYAVD
                     WNGIFDIPNISSYLDLLIIMGYDYYYSGSSNAGPTDPLYGFETGYNYCMSKSISYYLY
                     QGVPASKLLLGVPYYGRQYQTTANTIPAASTGSANSSTVTYRTVRVNSSGNYSNANKS
                     FDYNSYTPYYAFQSSGSWYQCFIDDSMSMRERYDIVNQRSLGGIGIWTLGYDDGYTEL
                     WNAINDKLTNCYAQSCTDSLFDMGGPNRNYHDKEIYTYTIAPDVTKNVNIQFSNFEFE
                     AGYDSLWLYDGPSTTSALIGAYSSTNSPGNITSTNHALTIKYKTDGATNKVGYKAYYT
                     CVGGDATPPNTAVTLPAWATANFTANYTDSDVGTGVQYRFYGVDNYTTSDFWRSNKGN
                     GFFNDDFSKATLHSDWASDAGTWSISSGAAYNSDEASSNTNLYVALPQTSNQLLYHWT
                     QTIGGSGTNRRAGVHFMCSVNTGNGRGNSYMIYARVDGGGKLQIYEYTADVMYLNADI
                     VYPFAANTTYDFKVMYNKLTGKISVWVNNVFAGSWTDATPITSGNYLCFRSGNCTMSI
                     DNVEVLKSRTGTSNMITVGAATTNDIQFENPMPSTPSGKVKSIVIDGADLISSIVSAT
                     INIDYSAPSMNAVRDGLGTSDINYFYFPGSISANWNAATEPSSNITNYYFAVGLTPGD
                     SNIINWTNNGTSRSVTANVPKMRKGITYYFTVKALNSLGMMSPPMNSNGQRLVYAKTV
                     SETTDTIIPNKKTDINTETSISVYPNPAKQQLTINYSLTETTWVLIDIFDLSGKKVAQ
                     VINKQLQDAGNYEEIYLLPDDLPSGNYLLTLQFGNKIKVLKFSVLK"
     gene            complement(4868..5572)
                     /locus_tag="PLF48_05335"
     CDS             complement(4868..5572)
                     /locus_tag="PLF48_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF48782.1"
                     /translation="MNTTTKKVLLVEDDPNLGLLLQEYLKHKNFEVDLKRDGVEGLFA
                     FRKNSYDMILLDVMMPKKDGFAVAEEIRKEDKHVPIIFLTAKSLKEDKIKGLTLGADD
                     YITKPFSMEILELKMNAILRRTERQEVQEVQDEYFAGNSRLDYKNQRLYIHGNEVKLT
                     TKENELLRLFFEKKNQVLEREIALKYVWHDDSYFTARSMDVFISKIRKYIKDDESLQI
                     LNIHGQGYKLVENATS"
     gene            complement(5680..7161)
                     /locus_tag="PLF48_05340"
     CDS             complement(5680..7161)
                     /locus_tag="PLF48_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF48783.1"
                     /translation="MNRNLLLVIAVLLFLGLLGIGTVQIFWLKSAVKSREQEFDKAVY
                     TSLNNISTKIEDINYKPLISAYLKRSYQGSISTNENGDIVLNWSDTSGREGSYKVKSD
                     IMPFSGGHMHMNYPGFAQTQDESYTELDNLQALMLQQMTSLQPINELIDTARLKTIIT
                     DELKAQGIKTKYYYGVTEYAPNNFVLLSKGTPLSELFKSDYSIDLFRRSMIDAGKTLK
                     LFFPDRKKFLYTSMSPLILSSAFFYLMIVAAFFLAFKIIFKQKKLSDMKTDFINNMTH
                     ELKTPIATISIASEMLRDNSISASEENRSKYAGIIFEENKRLYNHVEQVLQLARLEKD
                     ELQLNLDDADIHQLITTTAKRSQLRLEELGGKIELHLDALQTIVKVDEMHFTNMINNL
                     IDNAIKYNDKEPLIKIKTLNVPYGIQIEVCDNGIGIKKEDLERIFEKFYRVSTGNVHD
                     TKGFGLGLSYVKSIVDKLDGKIRVESKWKEGTKFIIQLPLDNV"
     gene            complement(7317..8648)
                     /locus_tag="PLF48_05345"
     CDS             complement(7317..8648)
                     /locus_tag="PLF48_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNF48784.1"
                     /translation="MADDKKEFQDKLPTSKVQRAGRVFKTTFKVGANYIKHAAQKVVN
                     DQIDEQALNEKNADELFKLMSQLKGSALKIAQMISMDNGFLPKQYAEKFAQAQNSAMA
                     LSAPLVVNTFKKYIGKTPQEIFDKFNPVAVHSASIGQVHKAEKDGLQFAVKLQYPGVA
                     ESIHSDINLVKPIFLRVIGIKEKEVQPYFDEFEERLNEECDYEKELHNAVFIAEKVKH
                     IPNLIIPKYYPQYSSKKILTMQWMNGMPLQQFLEQETNQDKKNKVGQALLDFLFYQIH
                     YLHQFHADPHPGNFLVSEDAQLIVLDFGCVKSISNEFYQNYFALANEKYVQDKNKLNQ
                     ILSHLDVLRPNDNPDKYTLFLETAKKAIALITYPTKFESFYFGDKNFYEKLQAQGEEI
                     FLNKEFRKPDAIRGSQHAIYLHRAFYGMYAILYKLNATVKVDNSFIEKIAI"
     gene            complement(8662..9144)
                     /locus_tag="PLF48_05350"
     CDS             complement(8662..9144)
                     /locus_tag="PLF48_05350"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48785.1"
                     /translation="MDNTILKPDWHAALHHTLEICEKDEQYPAYTVREKLLSFVFTFI
                     QTLQENENLFSSAISSNRLTCMNVVKADVQQYFEQLIWEGSNTGEIQSRLYLSSYYKD
                     AVWNMLLSVVNFWQRDQSILKERTDVYIEKSVHFTVDLLSPNALDSGFDLIQQFIKWR
                     "
     gene            9203..>9444
                     /locus_tag="PLF48_05355"
     CDS             9203..>9444
                     /locus_tag="PLF48_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNF48786.1"
                     /translation="MQEIERLRSEFLKYLEQESFRNEPHNLYEPNNYFLQIGGKRLRP
                     VLLLLTTELFGGDVNEALPASLAVEYFHNFTLIHDDV"
BASE COUNT         2898 a         1780 c         1663 g         3103 t
ORIGIN      
        1 taaaaaatca actatttttt aattgaaacg cttatcccga actcaggtta ataatttgaa
       61 tctgaagccg gtattttatg gctgtaacta ttcggttatt tcttttttct gaaacatcaa
      121 accgaacatc atcactatag ctgtcagcaa tatgacttga atcgtgagtg aattattgac
      181 caaattgacc gactgtcttt ccggaatatt gagaaataac aagatagtaa ttagtccgcg
      241 cggagcaata tacagaatgg gattcaaatt tattttgaat aacgtcagga aaataaaacg
      301 caagatatag atggtggcca agatgcccat tgtccatacg acagcatcga cattaagcaa
      361 ttcatttaaa ttaattaaga atccgaagag caagaaaaac aaggctctca ccaaaaaagc
      421 catttctata actagttctc taaatttatg tacttcttcc tccagaattt caggtttcaa
      481 tagctttata aaagaaacat ctttaaatac gtctaaattt ccgagtgata atccgaataa
      541 taaaataaaa agcagacccg gcagatgata gattttagtg attgcatata tcagaataat
      601 tatcagaata atcggtgcat attttacatg atgtttgatt ttactcaaca agaaagctaa
      661 accgagtgtg gcaattacgg tgataaccag catcagcata atttcaaaag taaatttggt
      721 aaatgacccg aaagtaatga cttcattgag cgtgatgaaa ttaaaaaaga tgactccgaa
      781 aatatcagaa agactgctct cataagtaac aaactctcta ttgctatcac ttagattttg
      841 agcactgggt attgcaatgg cactactgac tactgcaaaa ggtattgcat ttgccaatcc
      901 ggcttgaaaa gaaatattac cgaaatgact aaaaagcgct gcaaatattg cactaaaaat
      961 aagcataggc agcagggcta ccagcatcga tttgcgtatc agcggaatct tggatttatt
     1021 atattccagt tccagtgagc cttccaacac gattagaatt agtccaaccg tacctaaaat
     1081 gggcagaaga aaatttaaat caggaacttc aatttgaaaa tataaggtaa tctgtttaaa
     1141 taaaaatcca agcaataaca aaagaattac tgatggaatt ttagtctttg atgcactgat
     1201 atcaaaaaca taagccagca ataacaacac acaaataatg ataataatag ttacagtcat
     1261 atctatttaa atttaaaaat aaaccttaat gatcttggct aagataagat tttctgatta
     1321 tttaagtaca ctgaacttaa gcacctttat cttgtttcca aattgcaaag taagcagata
     1381 attgccggat ggaagatcat caggcaaaag gtaaatttct tcataattac cggcatcttg
     1441 tagttgttta ttgatgactt gtgccacttt ttttccgctt aaatcaaaaa tatcaattag
     1501 cacccatgtt gtttcagtca aagaataatt gatagtcaat tgttgttttg ccggatttgg
     1561 atacacacta atacttgttt ccgtatttat atctgttttc ttatttggga taatagtatc
     1621 agttgtttca cttactgttt tagcataaac taaacgctga ccatttgagt tcatcggcgg
     1681 actcatcatg cctaagctat ttaaagcttt tacagtgaaa tagtaagtaa tacctttgcg
     1741 catttttggc acatttgcgg ttacacttcg tgatgtacca ttattcgtcc aattgataat
     1801 attggaatct ccgggtgtaa gtccaacggc aaaataatag tttgtgatat tggaactcgg
     1861 ctcagtagct gcattccagt ttgcactgat ggaacccggg aaataaaaat aatttatatc
     1921 acttgtgccc aatccatcac gtactgcatt catggatggt gcactataat ctatatttat
     1981 agtagcagaa actatggaag aaattaaatc tgccccatct ataacaattg attttacttt
     2041 tccggatggt gtacttggca ttgggttttc aaactgaata tcatttgtag ttgcagcccc
     2101 tactgtaatc atattacttg ttcctgttct gctttttaat acttctacat tgtctatact
     2161 catcgtacaa ttgccgcttc tgaaacaaag ataatttccg cttgtaatag gtgtggcatc
     2221 tgtccaactt ccggcaaaaa cattattcac ccaaaccgaa atttttcctg tcaacttatt
     2281 atacatcact ttaaaatcat aagtcgtatt cgctgcaaat ggataaacga tatcggcatt
     2341 caaatacatc acatcggctg tatattcata aatttgcaac ttgcctcctc catctaccct
     2401 ggcatagatc atgtaggaat ttcctctgcc attgccggta ttgacactgc acataaaatg
     2461 cactccggcc ctgcggttgg taccgctgcc gccaatggtt tgagtccagt gatataaaag
     2521 ctgattggaa gtttggggta atgccacata caaattggta ttagaacttg cttcatccga
     2581 attataagca gcaccactgc tgatgctcca tgttcctgca tcgcttgccc aatcactgtg
     2641 tagtgtagct ttagaaaaat catcattaaa gaaaccattt cctttattag aacgccaaaa
     2701 atcagaagtg gtataattgt ccacaccata aaaacgatac tgaacacctg tacccacatc
     2761 gctgtctgta tagtttgccg taaaatttgc tgttgcccaa gcgggtaaag ttacagcagt
     2821 attcggtggc gttgcatctc cgccaacaca agtataataa gccttatagc caactttatt
     2881 tgttgcaccg tcagttttgt atttgatggt taatgcatga ttggtagaag taatattacc
     2941 cggtgaattg gtggatgaat acgcaccaat taaagcagat gtggtagagg gaccgtcata
     3001 taaccataat gaatcataac ctgcctcaaa ctcaaaattg gaaaactgaa tgttgacgtt
     3061 cttggtaaca tccggagcta ttgtgtacgt ataaatttct ttgtcgtggt aattgcggtt
     3121 gggccctccc atgtcgaata aagaatcggt gcaagactgt gcgtagcaat tggtgagttt
     3181 gtcattaatg gcattccaca actcggtata tccatcatca tagcccaagg tccaaatgcc
     3241 gataccgccc aatgaacgct gattgacaat atcatatcgc tctcgcatac tcatcgaatc
     3301 atctataaaa cactgatacc atgacccgct gctctggaac gcatagtaag gcgtataact
     3361 gttgtaatcg aaacttttat tggcattgga ataattaccg cttgaattta cccgaaccgt
     3421 gcgataggta actgttgaag aattggcaga tcccgtactt gcagcaggaa tcgtatttgc
     3481 cgtagtctga tattgccgtc cgtaatatgg aacacctaat aataattttg aggcgggtac
     3541 tccttgatac aaataatacg aaatagattt agacatacaa taattatagc cggtttcaaa
     3601 gccataaagc gggtcggtag gtcccgcatt cgaactgcca gagtaataat aatcatagcc
     3661 catgatgata agtaaatcca gatagcttga gatattcgga atatcaaaga ttccgttcca
     3721 gtccacggca tataaagcaa tggaaatttc gcaaccggga acagaatcgt gcatggtagt
     3781 acaaaactgt tggataaagg cggtgaggtt ggctttttgc gtagaggaaa cgccctcaaa
     3841 atcgagatta acaccgtttc cgcctctgct tcgcaatagt gaaatacaat tttgcgtaaa
     3901 tgtatttttg gctgttgttg aagttaagaa ggtggagtgg gatgaaaaca aagtggcaca
     3961 aaaggagatt ttaaccccat tgtttttggc agcggttact gccgaacttg ttgaccatgc
     4021 aaacgaagta ttgctgttgg ctccggtaga agggttgatg gtataatcga aataacataa
     4081 atcgcttatc agattccatt tgtaattatt gtgcagtgta ctgccattcc agtaaggatg
     4141 ccagccataa acacgtctgc cgttcaggct gcaagtcgct aaacgttgat tagtattgtc
     4201 gttgtgtagt tttttgaatt ttacacccgt cagttgctct tgcaatttat cccagtcggc
     4261 ttctttcgta tagccatatt tatcataaaa ggctttttgc tcttgcataa cagattgagc
     4321 ctgcaaaccg gataaaaaat agaagaagaa aataagtgta aaaaattgct tcatattaga
     4381 gggattgatg cctaacgaat atcgtaaatt attttataaa aacaaaatta gtacgagatt
     4441 aaagacataa taccacataa gtagaatggg acattttttt ttgtataagc cgactaactg
     4501 actatagact ccaaactcat ccaatcaaca aggtgtcgcc actacgtggc tttctgataa
     4561 gaataaaatt tataccacta ctaaactatt aaactattag actaattaac tattccacct
     4621 atgaaagtat tgccagtgcg tggctaattt ttatggtata tgttttttaa aaaaaggatt
     4681 tgctgttatg cgacaattta gcaattacca tttccctgaa aattagaaat atatacaaat
     4741 atagcaccca aaaaattccc gctcgaaaga agcataagta ggatagcatt tctcatgttg
     4801 aaactcctat aatcgctctt tagtcttcta aattgcaaga tgaaatgctg tcatcaaact
     4861 tcccaaccta cgaggtggca ttttctacca gcttgtagcc ttgtccatgt atattcagta
     4921 tttgcagact ttcatcatct ttgatatatt ttctgatttt ggaaataaac acatccatgc
     4981 tgcgtgccgt aaaataactg tcatcatgcc atacatattt caacgctatt tctctttcca
     5041 gcacttgatt tttcttttca aaaaacaagc gcaacaattc attttctttg gtagttagtt
     5101 ttacttcatt gccatgtatg tacaagcgtt ggtttttgta atccaatctt gagtttccgg
     5161 cgaaatattc atcttgcact tcctgcacct cttgacgctc tgtacggcgc aaaattgcgt
     5221 tcatcttcag ctctaaaatt tccatgctga acggcttggt gatatagtcg tcggcgccca
     5281 aagtcaaacc tttgatttta tcttctttca gcgattttgc cgttaggaaa ataataggca
     5341 cgtgcttgtc ctctttacga atttcttcgg caacggcaaa cccgtctttt ttcggcatca
     5401 tcacatcaag taaaatcata tcgtagctat ttttgcggaa agcaaacaaa ccttctacgc
     5461 catcgcgttt taagtctact tcaaaatttt tgtgtttcag atattcctgt aacaataaac
     5521 ccaaatttgg atcgtcttct actaaaagta ctttttttgt tgttgtattc atgatgttgt
     5581 atttagttgt ttgttattag tgattgggaa ttagttgttt gttacagtac attggtatat
     5641 tggtaaatta tcgaattagt acatcttcat tcaactttct taaacattat ccaatggcag
     5701 ctgtatgata aattttgttc cttctttcca tttgctttct acacgtattt tcccgtccag
     5761 tttatcaact attgatttta cataactcaa gcccagacca aaacctttgg tgtcgtgtac
     5821 atttcctgta ctcacccgat agaatttttc aaaaatgcgc tctaaatctt ctttcttaat
     5881 ccctatgcca ttatcacaaa cttcaatttg tatgccataa ggaacattaa gtgttttaat
     5941 ttttatcagc ggttctttat cgttatactt tatggcattg tctatcaggt tgtttatcat
     6001 gtttgtgaaa tgcatttcat ccacttttac tattgtttgc aaggcatcta aatgcaattc
     6061 tattttgcca cccaattctt ccagtcgaag ttgcgatcgt ttagctgtcg tagtgattaa
     6121 ttgatgtatg tcggcatcgt ccaaattaag ttgtaattca tctttttcca atcgggccaa
     6181 ttgaagcact tgttctacgt gattatataa tcgtttattt tcttcaaata taataccggc
     6241 atatttcgat ctgttttcct cagaagccga aatggaatta tcccgcaaca tctcactggc
     6301 gatggatatt gtggctattg gtgtctttaa ttcatgtgtc atgttgttga tgaaatccgt
     6361 tttcatgtct gatagtttct tttgcttaaa gatgattttg aaagctaaaa agaaggccgc
     6421 cactatcatt aaataaaaga acgcagatga caaaatcagg ggtgacatag aagtgtacaa
     6481 aaacttcttt ctgtcaggga aaaaaagttt caatgtctta ccggcatcaa tcatgcttcg
     6541 gcggaacaaa tcaatagaat aatcagattt aaacaactct gacaagggag ttcctttaga
     6601 caacaaaaca aaattattcg gtgcatattc ggttactcca tagtaatatt ttgttttaat
     6661 accttgagct tttaattcat ccgtaattat cgtttttaaa cgagcagtat ctatcaactc
     6721 atttattggc tgaaggcttg tcatttgttg caacatgagt gcctgcaaat tatcgagttc
     6781 agtgtatgat tcatcttgcg tttgagcaaa acccgggtaa ttcatgtgca tatgacctcc
     6841 ggaaaaaggc ataatatctg acttgacttt atagctgcct tctctgccgg aagtatcaga
     6901 ccagttcagg acaatatcac cgttttcatt tgtactgatt gaaccttgat aagagcgttt
     6961 caaataagca gatatcaagg gtttgtaatt gatatcttct attttggtag aaatattatt
     7021 aagtgaagta tagacagctt tatcaaactc ctgttctctg gattttacag cacttttcaa
     7081 ccagaaaatc tgaactgttc caataccgag caaacctaaa aagagaagta ctgcaataac
     7141 taaaagtaaa tttctattca caacttaaat ttacaacaga caaacatctt aaaatgttaa
     7201 caattcttaa ttgattgatt tttaattaca tagcattgta aatcacaatt ttatacaata
     7261 gctaaataca tatttaaatg aatgaaaagc cctgtttaca tttctgttct aaactatcag
     7321 atagctattt tttcaataaa gctgttgtcc acttttacgg tggcattgag cttgtataga
     7381 atggcataca tgccatagaa agcgcgatgt aaataaatgg catgctgcga gcctcttatg
     7441 gcatcgggtt ttctgaactc tttatttaaa aagatttctt cgccttgtgc ctgcagtttt
     7501 tcataaaagt ttttatcgcc gaaataaaag gattcaaatt ttgttggata ggtaatcaaa
     7561 gcaattgctt tcttggcagt ttctaaaaac aaggtatatt tatcaggatt gtcattgggt
     7621 cgcaatacat ccagatgaga taaaatctga ttgagtttgt ttttatcttg cacatatttt
     7681 tcattcgcca gagcaaaata attttgataa aattcgttgg aaatgctttt tacacaaccg
     7741 aagtccaata cgataagctg cgcatcttcg cttaccaaaa aattgcccgg atgcggatcg
     7801 gcatgaaatt gatgtaagta gtgaatttga taaaagagaa aatcaagaag tgcttgccct
     7861 actttgtttt ttttatcctg attagtttct tgttctaaaa attgttgcag cggcatacca
     7921 ttcatccatt gcatcgtcaa aattttctta gacgaatatt gcggataata ttttggaatg
     7981 ataagattgg gaatatgttt aaccttttca gcaatgaaaa cggcattgtg caattctttt
     8041 tcataatcgc attcttcatt cagccgctct tcaaattcgt caaaataggg ttgtacttct
     8101 ttttctttta taccaatcac tctgagaaaa atgggtttca ctaaattaat atccgagtga
     8161 atgctttctg ctactcccgg atactgcaac tttacggcaa attgcaatcc gtctttctct
     8221 gccttgtgta cttgtcctat cgaggctgaa tgcacagcta cgggattaaa tttatcaaag
     8281 atttcttgtg gtgttttgcc gatatatttt ttaaacgtgt tgactactaa cggcgcactc
     8341 aaagccatag cactgttttg agcctgagca aatttttctg catattgttt gggcaagaat
     8401 ccgttgtcca tgcttatcat ttgcgcaatt ttcagtgcac tgcctttcaa ttgactcatt
     8461 agcttaaata attcatccgc atttttttca ttcaatgcct gttcatctat ttggtcattc
     8521 actactttct gagccgcatg tttgatataa ttggcaccta ctttaaaggt ggttttaaaa
     8581 acgcgacctg cacgctgcac ttttgatgtg ggtagtttat cctgaaattc ttttttgtca
     8641 tctgccataa tacaaattag cttatctcca ttttataaat tgttgtatca aatcaaagcc
     8701 agagtccagt gcgtttggcg acaataaatc gaccgtgaaa tgcactgatt tttctatata
     8761 cacatctgtt ctttctttga gaatcgattg gtcacgctgc cagaaattta ctacagacaa
     8821 caacatattc caaacggcat ccttatagta agaggagaga taaagccggg actgaatttc
     8881 acctgtgttg ctgccttccc aaatcaattg ttcaaaatat tgctgtacat ccgcttttac
     8941 aacattcatg caagtcaatc tgttgctgct tattgccgat gaaaataaat tttcattttc
     9001 ctgcaaagtt tgaataaagg taaagacgaa ggagagcagt ttttctctca ccgtgtaagc
     9061 cggatattgt tcatcttttt cacatatttc cagcgtatga tgcagtgcag cgtgccagtc
     9121 aggtttcaaa atagtattgt ccatgtcgaa gttcaaaatt agttgtataa atcatttatc
     9181 tttgttacaa attgttaatg taatgcaaga aattgagcga ctgcgtagcg aattcctgaa
     9241 atatttagag caagaatcat tccgaaatga acctcataac ctgtatgagc cgaataatta
     9301 ttttttgcaa ataggcggaa aaaggctgcg tccggtttta ttattgctga ctaccgagct
     9361 ttttggaggt gatgtaaacg aggctttacc ggcatcgctg gcagtagaat attttcacaa
     9421 tttcaccttg atacatgatg atgt
//
LOCUS       DAOQII010000080         9385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3882_length_9385_cov_5.235584, whole genome shotgun sequence.
ACCESSION   DAOQII010000080 DAOQII010000000
VERSION     DAOQII010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_9385_cov_5.235584"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PLF48_05360"
     CDS             <1..147
                     /locus_tag="PLF48_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNF48787.1"
                     /translation="FEFIVDKAQEIYLFDTQLDFKGGLDNRGFIFENPNASSTCGCGT
                     SFSV"
     gene            289..570
                     /locus_tag="PLF48_05365"
     CDS             289..570
                     /locus_tag="PLF48_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNF48788.1"
                     /translation="MNKGDLVKIIADAGELTNAKATETLNAVLESIRKTLKKGDKVTL
                     IGFGTFSTSKRAKRQGRNPSTGAKITIPAKTLVRFKAGKELTDAVNKSK"
     gene            complement(726..1562)
                     /locus_tag="PLF48_05370"
     CDS             complement(726..1562)
                     /locus_tag="PLF48_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNF48789.1"
                     /translation="MRDELRNDALSIRASSISFFFILALFPGIIFLFSLIPYVPIKHF
                     DKLVLNFLSEILPNGVFPILESTIKDIVSVQRGGLTSINFFLALFVASGGVTSMMRAF
                     DKMNHTFKKRNFWQKRAASIRILVLVNTQLIIAVLFILKGKEVIQLLLTWLNAKDTII
                     EHVLKISKTIIIILSFFNTYALVYYFGPSVIKKYRYFSAGATFATAVSILMSYLFRIY
                     TSYLNNFNRLYGSLGVMIVIMMWIYLNAFVLLFGFEINHSIALNKALREDKSSEGLND
                     DN"
     gene            1872..3104
                     /locus_tag="PLF48_05375"
     CDS             1872..3104
                     /locus_tag="PLF48_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF48790.1"
                     /translation="MNVEWFIAKRMALGDKSSFSRFIIRIAIIAISLSVAVMIISACM
                     VTGFSKEIKKRIFGFWGEIHIENFEVSNAYETPPINTSQLQLNKLKQNAAIKNIAPYI
                     IKPTILKTKKEIEGIILKGVDSSYDWKSITPFIRQGRAIHYVKDTAAVKEILVSQTTA
                     DKLNIKLGDKVIAYFIKKEQSNTIGRKLVVVGIYHTGLDEYDKDFALVDMRLLQELNQ
                     WKQGDAAGYEVRLWDMNRMNELKDSIYYKYIGQELKAVTMREENLNIFEWLDLQRVNE
                     YLILGLMIIIAVLNMITALIILILDRTKMIGILKALGADNTSISTLFIYNALYIIVYG
                     LLWGNVIGLGFCIIQHYTGIIKLNEANYYLSVAPIYFNIPFILAVNVGTVVICLLILL
                     VPVQLISNISPLKSIRFE"
     gene            3122..4177
                     /locus_tag="PLF48_05380"
     CDS             3122..4177
                     /locus_tag="PLF48_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HNF48791.1"
                     /translation="MFKKIWSYKWILLTVCCFILLYTLPEHVIERYYSRGLFLVFRAF
                     FDYTFAFLPFPAYYLFLLFCVLTLIRWVLGFFRLNPLPPLTRVIRAFSFIGFMVVFFF
                     LIWGFNYGRTSVEKMLNLNLRPLTTDELRTETDTVLDQLHQARLKIKEDTNTLPQIVF
                     LNNIEDKSREDLNHVLEKNNYPYSDRLRGRFVFDNLFLIFSVGGQYMPYVGEGAVDDA
                     VFYSKKPFYVMHEMAHGNGITGEGDCNFWAYMSCMQSQNVAFQYSGSLNYLFYLLSEL
                     KLRDKQQYEEVYKNMPDVIKRDLNEIKEYDKKHTFFTGFLGDVFNTIYLKIMGVPDGV
                     RNYDKLVLLAYAWRHQQ"
     gene            complement(4205..7492)
                     /locus_tag="PLF48_05385"
     CDS             complement(4205..7492)
                     /locus_tag="PLF48_05385"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNF48792.1"
                     /translation="MRYYLQLFLSRFLIPFLFVTQWSFTQAQIYVGAWNPIRYPNSGV
                     CGWAFQDDNAAGTSSDPYASIEKALTTAINNNTPNQIIYILPDAYIQNWDYDSTLTEI
                     RDNCDGHDLDYTPIIGSSLNGLQILGTASGCQTMLEALGGSNSLQFIKIDGASNITIS
                     NVYFKGYSQAVSIDNSSNITFNNCVFESCNYGVTSLDGILIKSTSSNTNVSFNGCKFI
                     DNTKNTNTNALTISSNSTTASIFTTVSLSNCEFVCNQKDNSGGSLMIKGRTISTAAPT
                     TVNISGGVFANNNATSAAGRGGAIYIDNNCLVSINGTRFYKNSCQTTGVSAANDGGGA
                     ISIVSDNTGLTAAARTEVNITNALFYGNQVTGSGAGGAICLVGVSGHTNSNRPKLTIT
                     NTLFEKNKSASSGGAIYTAYGELSITNCTFKRDTALTGGAICIKQSGSVFTLVNDSFI
                     LNNATTGKDIQSDADFTMNNCLTTTANIAGPGTYNWVLMDNFNRTTNNTVGNGWTEAE
                     SAGSSGNRISVGHATITGASNQLVMNTGATIGREWSYKPQPSGMITNGITTNTAKIIW
                     AFNMVQNRSTVGGFDAGSYGIGYILGCTNTDITLGNGYAVVLGNNTGTASVNRTPRLV
                     KFTGGLDANANLTNVISGNALTNAYDYLSIKVEYNPVTDEWKLFQESGSAAYPRTDPR
                     LTSLQVGLTTTDNTYVGTSDNLANEGFIYNHSNGSAGSNYSFFDELYIQTSDGLGTGS
                     TLAYVDASNLVNSYDCTNGYCALEVAGTCLSNALSNFVCTASNATGSIGGIAFFDSNG
                     DGIYNENQPLDSIYILLLDNNNNVIARYTTDATGQYTFFGLPSGSYKVAFTKLGYFAG
                     QTLQNQGVGTTDSDIDNTFISQTVEINTSLSNTIADDNNTQALNFQNVSAGFVLNSIL
                     PVTLISFSGEKNSCREGLLKWTTANEIAFKEFNVMESEDGINYRIIKTIPAISSSGNQ
                     DYDVALDLINQNNYYKLKLTDLDYSFSYSNVLLLKNDCIAENSLNFSLYPQPANNILN
                     VFFNKTTEPNLIFIIYDLQGNIIKKQNAVNTSPTQPVDISTFQPGVYLFSVNSSSGII
                     VEKFIKE"
     gene            complement(7499..9001)
                     /locus_tag="PLF48_05390"
     CDS             complement(7499..9001)
                     /locus_tag="PLF48_05390"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNF48793.1"
                     /translation="MKKLIHLLLFVLIFVNTNAQLTVNTGISANALVGSLLGSGVTAS
                     NVSFQGVMGTGTKYQAGSFTASGTVATNLGITSGIVLSSGHVGDIPLSMSTFPGATSF
                     TSSGLTSTCSNGEIRQGGSCPVYINDVDVLAGNKNYYNAAILEFDFVPQNSIVSFSYV
                     FGSEEYDQNDGSGFPVNYNCSSYNDKFGFIISGPGISGGQGYTNNGKNIARLGNGAEV
                     SINSVNDGIVGASGGSPSASVCTAANPAWTNGSPTTEFFGPIYGIQFNGNTKKLSAYI
                     SGLTAGSTYHIKLIVTDVNDGAYDSGVFLQAASFTSPVILPVELVSFSAACKENYTEI
                     NWSTATEKNNDYFILQKAASDFIFKDIATLNGKGTTAIEQAYTYKDYEKSANSYYQLV
                     QVDADGMKHNSKIIYNKQECDNENNLSEITWHSATNSIHIQLQSNQINNAELIVTDLN
                     GKLIFRQSFAYTAGLNELDFNLKYINNGMYICTITGEQIIKSKKLLINTK"
     gene            9146..>9385
                     /locus_tag="PLF48_05395"
     CDS             9146..>9385
                     /locus_tag="PLF48_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48794.1"
                     /translation="MSEKRQFKLYSNRHVIQKEKQYVVLFDVLDRFHEFDEHLLMEQL
                     AETGYSIRFLKASIHYLYDLILKSLVLFHSGKTVTL"
BASE COUNT         2944 a         1618 c         1727 g         3096 t
ORIGIN      
        1 tttgaattta ttgtagacaa ggctcaggaa atttatttat ttgacacaca attagacttt
       61 aagggcggtt tggacaaccg tggctttatt tttgaaaacc ccaatgcatc gtctacttgt
      121 ggctgtggta catcattttc ggtgtaaata tcaataaaat aaggcatttt gctattaatt
      181 tgtacacagc atccttattt tattaacatt ctgaaaattt aatttgcaga attgaccaaa
      241 cgatttattt ttgtgtcata atcattcata actaaaaatt ttaactttat gaacaaaggt
      301 gacttagtaa aaatcattgc agatgcaggt gagttgacca atgcaaaagc aacagaaacg
      361 ttgaatgccg tattagaaag cattcgcaaa acgttgaaaa aaggtgacaa agtaacatta
      421 atcggtttcg gtactttctc aacatctaaa agagcaaaaa gacaaggaag aaacccatct
      481 acaggtgcta aaatcactat tcctgcaaaa acattagtgc gtttcaaagc aggtaaagaa
      541 ttgacagacg ctgtaaataa aagcaaataa taaactactg ctttttatag aaaatccctg
      601 atgtgaatca gggatttttt attaggtgaa aactgatgca attctattaa gtactgattt
      661 tattctttaa ataagataaa cgtgaatttt aaaacagatg acttgccaag caaataatgg
      721 aaaagctagt tgtcatcatt caatccttcc gagcttttgt cttcgcgaag ggctttgttg
      781 agtgctatac tatgattgat ttcaaagccg aaaagaagta cgaaggcatt cagatatatc
      841 cacatcatga tgactatcat cacgcccaaa gagccataga gcctgttgaa attattgagg
      901 taagacgtgt agattctgaa taaataggac atgagaatgg aaactgcggt agcaaatgta
      961 gcccctgcgg aaaaataacg gtatttttta attactgaag ggccgaaata atagactaat
     1021 gcatacgtgt tgaagaaact aagaataatg ataattgttt ttgaaatttt taatacatgt
     1081 tctattatcg tgtcttttgc attaagccag gttagtaaga gttgtatgac ttcttttcct
     1141 ttcaaaataa ataatacggc aatgatgagt tgtgtgttga ccagtacgag aatgcgaatg
     1201 gaggcggctc ttttttgcca gaaatttcgt tttttaaagg tgtgattcat tttgtcgaag
     1261 gcacgcatca tagaagtaac accacccgac gcaacaaaca aagccagaaa aaagttgatg
     1321 gaagtaagcc cgccgcgctg tacgctgacg atgtctttaa tcgtcgattc taatatggga
     1381 aaaacgccat taggcaaaat ttcactcaga aaatttaata ccagtttgtc aaagtgcttt
     1441 atgggtacat agggtattag tgaaaataaa aaaataatac ccggaaatag agcgaggata
     1501 aaaaagaaag aaatagatga agcccttata ctcaaggcat cattccgtaa ttcgtcacgc
     1561 agctttacta atacatcata cagtggaatg ccttcaaatc cgggtaagac cagatttttt
     1621 attcgttgct tgaaacgctc agaacgaacc agacgattgt atttgtcaaa agtagaaata
     1681 aaaaatagct ttatttttca atatacgaaa atacaaaatt atctttcgtc acaaaaggct
     1741 gatgtattta gtaataaaat gcaatttctg taccaaataa gaattagtct gcattaaatc
     1801 cgtaattaac ctttttcgta aaaaatgagt aatctttttc aggcttcttg taaatttccg
     1861 atattcgcag ggtgaatgta gaatggttta tagcaaaaag aatggcattg ggcgacaagt
     1921 cgtctttttc ccgttttatc attcgcattg ccattattgc tatttcgctt agtgtggcag
     1981 ttatgattat ttcggcatgt atggttaccg ggttttccaa agaaattaaa aaacgcatat
     2041 ttggattttg gggcgaaata catattgaaa atttcgaggt ttcaaacgcc tatgaaacac
     2101 caccgattaa tacaagtcaa cttcagctta ataagcttaa gcaaaatgca gctatcaaga
     2161 atattgcacc ttatatcatc aaaccaacta ttttaaaaac gaaaaaagag attgaaggaa
     2221 ttatactcaa aggcgttgat tcgagttatg actggaaaag tattacgccg tttatccggc
     2281 aaggacgtgc tattcattat gtaaaagata cagctgcagt aaaagaaatc ttagtatcac
     2341 aaaccactgc agataagctg aatatcaaat taggagataa agtaattgct tattttatta
     2401 aaaaagaaca aagcaatact attggcagaa aactggtggt agtgggcatt tatcataccg
     2461 gcctggatga gtatgataaa gattttgcat tggtggatat gcgcttactg caggaactca
     2521 accagtggaa acagggcgat gcggcaggtt atgaagttag gctttgggat atgaaccgaa
     2581 tgaatgagct taaggattca atttattata aatatattgg acaggaactc aaggctgtaa
     2641 cgatgcggga agaaaacctc aatatctttg aatggctgga tttgcaacgg gtaaatgaat
     2701 atctaatctt aggattgatg attattatcg ctgtactcaa tatgataacc gcattgataa
     2761 ttttgattct ggatcgcact aaaatgatag gcatcctgaa agcacttgga gctgacaata
     2821 catctatttc taccctgttt atttataacg cactgtatat tatcgtttat ggattgcttt
     2881 ggggaaatgt aataggtttg ggtttttgta taatacaaca ttatacaggc attataaaat
     2941 tgaacgaagc caattactat ttatccgtag cacctattta ttttaatatc ccgtttattc
     3001 tggctgtcaa tgtaggaacg gtagtgattt gcctgcttat cttattagtt cctgttcagc
     3061 ttattagcaa tatttcacct ttgaaatcca tccgatttga ataatttttg tattttgtca
     3121 gatgttcaaa aaaatctggt catataagtg gatattgctg accgtttgct gttttattct
     3181 tttatataca ttaccagaac atgtaattga acgctattat tctagagggt tatttttagt
     3241 atttcgcgca ttttttgatt atacttttgc ctttctgcca tttcctgctt attatttatt
     3301 cttgttgttt tgtgtattaa cgctaattcg atgggtattg ggctttttca gacttaaccc
     3361 attgccaccc ttaacaagag taattcgtgc attttcgttt atcggtttca tggtggtatt
     3421 cttcttttta atctggggat ttaactacgg aagaacttcg gtggaaaaaa tgcttaatct
     3481 gaatctaaga ccgcttacta ctgatgaatt aagaacggaa actgatacgg ttttggatca
     3541 attgcatcaa gcgagactga aaataaagga agacacaaat accttaccac aaattgtttt
     3601 cctgaataat attgaagata agagcagaga agatttgaat catgttttag aaaaaaacaa
     3661 ttatccgtat agtgatcgct tgcgtgggcg atttgttttt gataacttat ttttgatttt
     3721 ttctgtagga ggacaatata tgccatacgt aggggagggg gctgttgatg atgctgtctt
     3781 ttattcaaaa aaaccatttt acgtcatgca cgagatggct catggcaatg gcattacagg
     3841 tgaaggcgat tgtaattttt gggcatatat gtcatgtatg caatcacaaa acgtagcatt
     3901 tcaatatagt ggcagcttga attatttatt ctatttgctc tcagaattaa aattaagaga
     3961 caagcaacag tatgaagaag tttacaaaaa tatgccggat gtaataaagc gggatcttaa
     4021 cgaaataaaa gagtatgaca agaaacatac attttttacc ggatttttgg gggacgtatt
     4081 caatactata tatttaaaaa ttatgggagt gcctgatggc gttcgcaatt acgataagct
     4141 cgttttactg gcttatgcct ggaggcatca acaataatgc aaaaataagg tttgtgttta
     4201 gctattattc tttaataaat ttttcaacga ttatgccact tgagctattt acagaaaata
     4261 aataaacgcc cggttgaaat gttgaaatat ctactggttg ggttggagaa gtattgactg
     4321 cattttgttt tttaatgata ttgccttgta aatcatagat gataaaaatg agatttggtt
     4381 cagttgtttt attaaagaat acattcagga tattgtttgc cggctgagga taaagcgaaa
     4441 agttcaaact attttcagca atacaatcat ttttcagcag caatacattt gaataagaaa
     4501 atgaataatc taagtctgtt agttttaatt tgtaataatt gttttgatta atcaaatcta
     4561 atgctacatc atagtcctga tttccacttg aggatatagc aggaattgtt tttattatcc
     4621 ggtagtttat gccatcctcg ctctccatta cattaaattc tttgaaagca atttcatttg
     4681 cagtcgtcca ttttaaaaga ccttcccgac aagaattctt ttcgccacta aatgaaatta
     4741 aagtaacggg taatatagaa ttaagtacaa aaccagccga aacattttgg aaattaagag
     4801 cttgcgtatt attgtcgtca gcaatcgtgt tactaagact cgtgtttatc tctactgttt
     4861 gagaaataaa ggtattgtca atatcactat cagtagtacc gactccttga ttttgtaaag
     4921 tttgcccggc aaagtagcct agttttgtaa aggctacttt ataacttccg gatggcagtc
     4981 caaaaaatgt atattgccct gtcgcatccg tagtgtatct ggctattaca ttattattgt
     5041 tatccagcaa taaaatatat atgctgtcca acggttgatt ttcattatag atgccgtcgc
     5101 cgttgctgtc aaaaaatgca ataccgccaa tggaaccggt ggcatttgat gcagtgcata
     5161 caaaattaga cagcgcattt gacaagcaag ttccagctac ttccaatgca caataaccgt
     5221 ttgtacagtc ataactgtta actagatttg atgcatcaac atatgctaat gttgaccctg
     5281 ttcctaagcc gtcactggtt tggatatata attcatcaaa gaaagaataa ttgctccctg
     5341 cggatccatt ggaatgatta tatataaacc cttcattagc taaattatca cttgtgccaa
     5401 cataagtatt gtcagtcgtc gtaagcccta cttgtaaact ggttaatctt ggatctgttc
     5461 tgggatatgc agcagagccg gattcctgaa acagtttcca ttcatctgta accggattat
     5521 attctacttt tatactcaaa taatcatagg catttgtcaa tgcattgcca ctaattacat
     5581 tagtgaggtt ggcattggca tctaatcctc ccgtaaattt tactaatcga ggtgttctgt
     5641 ttacacttgc agttccagta ttatttccta aaacaacagc gtatccgttt cctaaagtta
     5701 tatcagtatt tgtacagccc aatatatatc caattccata actcccagca tcaaatccac
     5761 caactgtact tctgttctgc accatgttga atgcccaaat tatttttgct gtatttgttg
     5821 ttattccatt tgtaatcata cctgacggtt gaggtttata agaccactct ctgccaattg
     5881 tagcacccgt attcataacc aattgattgg aagctcctgt aatggttgca tgtcctactg
     5941 aaatacgatt tccggaagaa cctgctgatt ctgcttcagt ccatccattg cctactgtat
     6001 tattagtggt tctgttaaag ttatccataa gcacccagtt gtaagttccg gggcctgcaa
     6061 tatttgcagt ggtggtaagg caattattca tcgtgaaatc tgcatctgac tgaatgtctt
     6121 ttcctgttgt tgcattattt aatataaatg aatcattgac taaagtaaag acactgccgg
     6181 attgttttat acaaattgct ccgccagtta gagccgtatc acgtttaaat gtacaatttg
     6241 tgattgatag ttctccataa gcagtataaa tagctccgcc gctcgatgct gatttgtttt
     6301 tttcaaatag cgtattcgtt atcgtcaatt ttggtcggtt tgaatttgta tgaccgctta
     6361 caccaaccaa acaaattgct cctcctgcac cggatccagt tacttgattg ccataaaaaa
     6421 gtgcattggt tatatttact tcagttctag ctgctgcggt caatccggta ttgtcagaaa
     6481 caatggaaat tgcacctccg ccatcattgg ccgcactgac tccggtagtt tgacaactgt
     6541 ttttgtaaaa gcgtgtaccg tttatactta ccaaacaatt attatcaata tagattgccc
     6601 cgccacgtcc tgcggcactc gttgcattgt tattcgcaaa tacgccaccg gaaatgttga
     6661 ccgtggtggg tgccgctgta gatatagttc tgccttttat cattaaagaa ccacctgaat
     6721 tatctttttg gttgcagaca aactcacagt tgctaaggct cacagtcgtg aaaatacttg
     6781 ctgttgttga atttgagctt atcgtaagcg cattcgtgtt tgtatttttt gtattgtcta
     6841 taaatttgca tccgttaaat gatacattcg tattgctgct tgtcgattta attaaaatgc
     6901 catcaagtga agtaactccg taattacatg actcaaatac acagttgtta aacgtaatgt
     6961 ttgatgaatt atctatggat actgcttgcg aatagccttt aaaatacaca ttggatatag
     7021 ttatattgct ggcgccgtcg attttaataa attgcagact attgctacct cccaatgctt
     7081 ccagcatagt ttggcatccg ctggctgtac ctaaaatttg caggccgttc aatgagctgc
     7141 caatgatagg cgtgtaatcc aaatcatgtc catcacaatt gtctctaatt tccgttaagg
     7201 tggagtcata atcccagttc tgaatatagg catcaggtaa aatatagatg atttggtttg
     7261 gcgtattatt gtttatggca gttgtcagtg ctttctcaat ggaggcatac gggtcggatg
     7321 acgttccggc agcattatca tcttggaatg cccaaccgca aacaccagag ttggggtagc
     7381 gaatcggatt ccatgcacca acatatattt gtgcttgtgt aaacgaccat tgtgtaacaa
     7441 acaaaaatgg aattaaaaaa cgagatagaa atagttgtaa ataataacgc atgtccatct
     7501 atttagtatt aattaatagc ttcttgctct ttattatttg ctcacctgta atggtgcaga
     7561 tatacatgcc attattgatg tattttaaat taaaatctaa ttcgtttaac cctgctgtgt
     7621 atgcaaatga ctgtctgaaa atgagttttc cgtttaagtc cgtcacgata agctctgcat
     7681 tattgatttg atttgattgt aattgtatat gaattgaatt tgtagcagaa tgccatgtaa
     7741 tttcagataa attgttttca ttatcgcatt cctgtttgtt gtaaatgatt ttgctgttgt
     7801 gcttcatacc atcagcatct acttgtacaa gctgataata ggaatttgca ctcttttcat
     7861 aatctttata tgtgtaagct tgttcaatag ctgttgttcc tttgccgttt aaggttgcta
     7921 tatctttgaa gataaaatca cttgcagctt tctgtaaaat aaaatagtca ttgttttttt
     7981 ctgtagccgt cgaccagttt atttccgtat aattttcttt acatgcagct gaaaaagaaa
     8041 ctaattcaac aggcaagatg accggagagg taaaacttgc agcttgcaaa aatactccac
     8101 tgtcgtatgc gccatcattt acatcagtaa caattagttt gatatgataa gtagatccgg
     8161 cagttaatcc gctgatatag gcacttagtt tttttgtatt tccattaaac tggatgccat
     8221 atataggacc gaaaaattcg gttgtcggac tgccattcgt ccatgctgga tttgcagctg
     8281 tgcatacaga agctgaaggt gaacctccgg atgcgcctac tattccatca ttaaccgaat
     8341 tgatagatac ctcagctcca tttccaaggc gggcaatatt ttttccatta ttggtataac
     8401 cttgcccacc gctaattccg ggtccactga taataaaccc gaatttatca ttgtatgaag
     8461 agcaattgta gttaaccgga aatccgcttc catcattttg atcatattct tcagacccaa
     8521 aaacataact gaacgaaaca atgctgtttt gaggaacgaa atcgaattct aaaatagcgg
     8581 cattgtaata atttttattc cctgccagta catctacgtc attaatataa acagggcaag
     8641 agccaccttg tctgatttca ccatttgaac atgttgaagt aagtcctgag ctggtaaaac
     8701 ttgttgctcc gggaaatgta gacatactaa gcggaatatc acctacatga ccactgctta
     8761 gcacaatacc acttgttata cccaagttgg ttgcaacagt tccgctagca gtaaatgacc
     8821 cagcttgata tttggtacca gtacccatta caccctgaaa acttacatta gatgcagtta
     8881 cacccgaacc taagagtgaa ccaactaaag catttgcaga tatgcctgtg tttactgtta
     8941 attgtgcatt agtatttaca aatattaata caaataataa aaggtgtata agtttcttca
     9001 tattaaaaaa aatgctagta ctttatcttt caaatttaac atagtgaaag ttggaaattt
     9061 tgtaaaaatg caatattttt agtaagtctg attttgtaaa atgaaaaatt ataatactgt
     9121 gtttcaatta gttaagtcta tgagtatgtc agaaaaaaga caatttaaac tctatagtaa
     9181 ccggcatgtc attcaaaaag aaaaacagta tgtcgtattg tttgatgtat tagatagatt
     9241 tcatgaattt gatgaacatc ttttgatgga acaacttgca gaaaccggtt attccatccg
     9301 ttttttaaaa gccagtatac attatctgta tgatttaatt ttgaaatcgt tagtgctttt
     9361 tcattcagga aaaacagtga ctctt
//
LOCUS       DAOQII010000081         9270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3949_length_9270_cov_4.960282, whole genome shotgun sequence.
ACCESSION   DAOQII010000081 DAOQII010000000
VERSION     DAOQII010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_9270_cov_4.960282"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLF48_05400"
     CDS             complement(<1..1112)
                     /locus_tag="PLF48_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNF48795.1"
                     /translation="MQQRKKLVIIGGGPAGFFAAIHAAKAHPDWFILILEQGNRVLGK
                     VKISGGGRCNVTHACFDTRELTQFYPRGQKELLAPFKLFACADTLHWFEERNVPLKTE
                     QDGRVFPVTDDSQTIIDCLLNEAKKNQIEIRKNCKVFSISHQQKFSIKTDREALEADV
                     VIVACGSSSLIWETLKEMGHTVIPPVPSLFSFKTKNTLFRNLSGIAIPNCSIKTNADK
                     VSVNGNVLITHEGISGPAILKLSSFSARTLAEKNYQFDCCINWCNAKAEDCMILLQEI
                     KSAHSKKQIHNFAPYDFPARFWENVLLENKIPHQKLWADISKNELQKLAQFLTNCTIQ
                     ISGKSTNKDEFVTAGGIKLSEVDFKTMQSKLIPGLY"
     gene            1150..1677
                     /locus_tag="PLF48_05405"
     CDS             1150..1677
                     /locus_tag="PLF48_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HNF48796.1"
                     /translation="MEIKFSQTLTYEAYKEFFKTFFLKKTKLSTKITYAVIFLIGLLN
                     LIFRIIIQKQADLTNIWVTVAFCGFPFLMYYASIRNMKSNYKKAAENFTNVAYLFMTD
                     RFSVTASNKNKTEMLYDNLFSFEETPNLYLLFISDNTAYIIDKNQIPDSEQAAFSQLF
                     HKISAKKTAGEHLVD"
     gene            1686..2387
                     /locus_tag="PLF48_05410"
     CDS             1686..2387
                     /locus_tag="PLF48_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48797.1"
                     /translation="MKSFRIFGLVFIVVLYTGFYFSEIRNKKKELNPVPVELNSIYVL
                     SKQNAHPNAVRLFTDEFYWNEADEISPFGNDNGWDALQQFVNWRKLNKQKSCVDFVNL
                     LFKQWGFPEFNIYTLDTLEIRSFVLKIANDSNLITKEMEVSIEKSAKEMAEDVNKPYN
                     KGIADSINRVVQNIMGYISISSMDDAIIATGFGQYLLEGKIDKDILELSKIALKRQLI
                     PDVTLMLTCLLRQNL"
     gene            2583..4661
                     /locus_tag="PLF48_05415"
     CDS             2583..4661
                     /locus_tag="PLF48_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNF48798.1"
                     /translation="MKLQFLFLIIFSSQFLFAINKQNIEIIRDAWGVPHIYAHTDEEA
                     AYGLAWATAEDDFKSMQENFLTARGRLAEVKGKEGAMMDFLCAFVGARETTEKYFDSA
                     FTPKFRAMLAAYAQACNDFAAKNPDKVWIKDAFPVNEKDIITGYVLGFALMTNVPYSV
                     MKISDGTIKTDNLLAPKGSNGFALNSNKTMDGNVYLGINSHQPLEGPYSWYEAHVHTD
                     EGWDMLGATFPGGVTLFHGANQHLGWAHTVSFADHDDVYLLTTRKQDGKIQYKYDGQW
                     QELKVKKVKLKVKLFWFIKIPVTKKYYESVYGPVLEHDGKYFAVRFPSAFDIRAAEQW
                     YRMNKAKNFSEFTDALKMMAFPGLNVIYADKENNIMYADMGQFPYKNRSYDWWHTLPG
                     DTSATFWKANNYWPYDSLIKIINPACGYVHNNNNTPFYCTAFDENCVKHYHPLEKDYF
                     FLENNRSIRTQYLFAHSGKLSYDDFKKIKYDVSFMNPAYNYAMENIEDLLHLSPEKYP
                     QIKQTIAVLSKWNRSADVDNKQAALVAFVIKKVIDQLIKDGNFPAVQARVKEPFLAKC
                     VEDAQHHFLKYFGKLEIPLGDFQKLVRGNKALPVAGVPDVIAAMYVKEYQDGMYKAEV
                     GETYIELVQFTKNGPIIESISPYGASNVEGNKHYDDQMDLFVKQQCKPMSMEKKIILQ
                     TAEKVYHPNM"
     gene            4810..5472
                     /locus_tag="PLF48_05420"
     CDS             4810..5472
                     /locus_tag="PLF48_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNF48799.1"
                     /translation="MANSNLFTSSVGRKIIVGLTGLFLITFLVVHLGCNLTIIMDSSG
                     NMFNDVAYFMSHFWLMRIIEVGLFLGLIAHIVMTLKLNFENKSKRPVGYAVSPGNVNS
                     TWYSRSMAILGSLLLIFLVIHLYQFWIPTKKALYITHEDEDAYTMVTEALRNPLVLLV
                     YIIAFISLGYHLLHGFQSAFQSLGLNHKKYTPIIKKTGTAFAIIVPALFALIALVVFF
                     TK"
     gene            5482..6003
                     /locus_tag="PLF48_05425"
     CDS             5482..6003
                     /locus_tag="PLF48_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNF48800.1"
                     /translation="MVNVSEHIILNKIYIIRDKKVMIDRDLAEMYGVETRVLNQAVKR
                     NSERFPEDFMFRFNEQDFENWKSQVVISNKEKMGIRNMPYAFTEQGVAMLSSVLNSKQ
                     AIQVNIQIIRIFTKMRELLLSHKEVLLKLNQLEKKLTQHDDDIAAIFNVLKQLLSPKN
                     KPTQKIGFKIGKQ"
     gene            6031..8010
                     /locus_tag="PLF48_05430"
     CDS             6031..8010
                     /locus_tag="PLF48_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNF48801.1"
                     /translation="MTLNSKIPAGPIDKKWSDYKGHVKLVNPANKRKLEIIVVGTGLA
                     GASAAASLGELGYRVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIK
                     GGDYRAREANVYRLAEVSVNIIDQCVAQGVPFAREYGGYLNNRSFGGTQVKRTFYAAG
                     QTGQQLLLGAYAALERQVALGSVKQYTRHEMLDVVIIDGKARGIITRDLVTGKIERFF
                     GHAVVLASGGYGNVFYLSTNAMGSNVTAAWKAHRKGAYFGNPCFTQIHPTCIPVTGDH
                     QSKLTLMSESLRNDGRIWVPKMKDDPRKANDIPEDERDYYLERRYPAFGNLVPRDVAS
                     RAAKERCDAGYGVGTTKQAVYLDFRYNLINKYGRAEASKHGIENPDEATLVRLGKEVV
                     KEEYGNLFDMYEKITGENPYEVPMRIYPAVHYTMGGLWVDYELMTTVQGLYALGECNF
                     SDHGANRLGASALMQGLADGYFVVPYTIGNYLADEIATQPISIDHPAFVEAENGVQER
                     INKLMNVKGSKSVESFHKRLGRIMWNKCGMARNAHDLQEAIEEIKALRKEFWSDVKIP
                     GGVNEINPELDKAGRVADFLELGELMCLDALDRNESCGGHFREEYQTEDGEALRDDVH
                     YKYVALWDYKGDSNWSLSKEDLVYENIKIAARSYK"
     gene            8052..8417
                     /locus_tag="PLF48_05435"
     CDS             8052..8417
                     /locus_tag="PLF48_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF48802.1"
                     /translation="MDAKENIIVKKCVEFSLDVIEYSEVLESKRKFMIANQLLKSATF
                     VGANVFEAQHSESKADFIHEMKIAMKEVNETIYWLIICEQAKSYPPPLNLKIKIEEIF
                     KIISKIIATSKKKEQTLSN"
     gene            8449..9213
                     /locus_tag="PLF48_05440"
     CDS             8449..9213
                     /locus_tag="PLF48_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNF48803.1"
                     /translation="MEHYNMNLTLKVWRQKNKNDKGGFETYNVKNISSEMSFLEMFDV
                     LNEQLVNENKEPIAFDHDCREGICGMCSMYINGRPHGPWAGTTTCQLHMRAFKDGDTI
                     TIEPWRSRAFPVLKDLIVDRTPFERIMQAGGFISVNTGVTIDANTILVPKDVADSAFA
                     AAACIGCGACVAACKNASAMLFVSAKVSQFALLPQGHPERRQRVLNMIAQMDKEGFGS
                     CTNTGACEAECPKQISLENIARLNREYFSASLASEE"
BASE COUNT         3086 a         1471 c         1815 g         2898 t
ORIGIN      
        1 aaatataatc cgggaataag cttgctttgc atcgttttaa aatcaacttc actcagttta
       61 atacctcctg ctgtaacaaa ttcgtcttta ttggtggatt tgccagaaat ttgaatcgta
      121 cagttagtta aaaattgtgc cagtttttgc agttcattct tgctgatatc tgcccataat
      181 ttttgatgtg gtattttatt ttctaacaac acattttccc aaaagcgcgc aggaaaatca
      241 taaggtgcga aattgtgaat ttgttttttt gaatgagctg attttatttc ttgcagtagt
      301 atcatacaat cttccgcttt agcattacac cagtttatac agcaatcaaa ttgatagttt
      361 ttttctgcca aagttcttgc agaaaaggat gataatttta aaatagccgg accacttatt
      421 ccttcatggg taattaaaac attgccattt actgaaactt tatctgcatt ggttttaata
      481 gagcaatttg ggatggctat gccggataaa tttctgaata gcgtattttt agttttgaat
      541 gaaaataagc tcggaacagg aggaattacc gtatgtccca tttctttaag ggtttcccaa
      601 attagtgaac tgctgccaca tgcaacaatc acaacatcgg cttctaaagc ttcccggtct
      661 gtttttatgc taaatttttg ttgatgagaa atggaaaaaa ctttgcagtt ttttctgatt
      721 tctatttgat tttttttggc ttcatttagt aaacaatcta ttatggtttg tgaatcatct
      781 gtaacaggaa ataccctgcc atcttgttct gttttgagtg gcacatttct ttcttcaaac
      841 caatgcaggg tatcggcaca ggcaaatagt ttgaatggtg ccaataactc cttctgtccg
      901 cgcggataaa attgggttag ctcgcgggta tcaaaacaag catgagtaac attacatctt
      961 ccgccacccg atattttaac cttccctaaa acacgattgc cttgttctaa aatgagaatg
     1021 aaccaatcgg gatgtgcttt tgcagcatgt atagccgcaa agaatccggc ggggccacct
     1081 ccaataatta ctaatttttt tctttgctgc acgatgcaaa tatctgaatt tacacttact
     1141 tttattacaa tggaaattaa attctcacaa acactcacct acgaagcgta caaggagttt
     1201 tttaaaacct tctttttaaa gaaaacaaag ctgtcaacta agattactta tgctgtcata
     1261 tttttaatcg gattacttaa tcttattttc agaattatca tccaaaaaca ggctgattta
     1321 accaatatct gggttacagt tgctttttgc ggtttccctt ttctaatgta ttacgcttca
     1381 attcgcaata tgaaaagcaa ttataaaaag gcagctgaaa attttacgaa tgtagcatat
     1441 ttatttatga cagaccgctt tagtgtaacg gcttcgaata aaaacaaaac ggaaatgttg
     1501 tatgataatt tgttttcgtt tgaggaaaca cccaatttgt atttgttgtt tatcagcgat
     1561 aatacagcat acattattga taaaaaccaa atacccgatt cggagcaagc agcattttca
     1621 caactgtttc ataaaataag tgcaaagaaa acagcaggag aacatctggt ggattaagtt
     1681 agaatatgaa atcatttagg atatttggct tggtatttat agtagtatta tatacaggtt
     1741 tttatttttc tgaaatacgt aacaaaaaga aagaattaaa tccagttcct gttgaattaa
     1801 atagtattta tgttttgtca aaacaaaatg cacatccaaa tgcagtaaga ttatttacag
     1861 atgaatttta ttggaatgaa gcagacgaaa tttcaccttt tggaaatgat aatgggtggg
     1921 atgctttaca gcaatttgta aattggagaa aattgaataa acaaaaatct tgtgtagatt
     1981 ttgtaaatct tttatttaag caatggggat ttcctgaatt taatatatat accctagata
     2041 cacttgaaat acggagtttt gtcttaaaga ttgctaatga ttcaaatctg attacgaaag
     2101 aaatggaggt aagtatcgaa aaatcagcaa aagaaatggc agaagatgta aataaaccat
     2161 ataataaagg aatagcagat agtatcaatc gagttgtaca aaatataatg ggttatatta
     2221 gtatatcatc aatggatgat gctattattg ctacggggtt tggtcaatat ttattggaag
     2281 gaaaaattga taaagatatc ttagagcttt ctaaaattgc attgaaaaga caattaattc
     2341 ctgatgttac tttaatgctt acatgtttgc tccggcagaa cctataaagc taagacttca
     2401 tttaatgcaa aagaaattag atgagataag tataaattaa attaatttgt ataatactaa
     2461 aatactaatt atgtcttact taatttaagt cattagttat tgagatgctt ataaaaaaat
     2521 cattccattt tttatttcca gtagaaatac ctattttaat ctaaatattt gctgtgaaaa
     2581 ccatgaaatt acaattctta tttttaatta tttttagttc acaatttctt tttgctatta
     2641 acaagcaaaa tattgaaatc attcgtgatg cttggggagt acctcatatc tatgctcata
     2701 cagatgagga agctgcttat gggttagcat gggctacggc agaagatgat tttaaatcta
     2761 tgcaggaaaa ttttctgact gcacgaggaa gactggcaga agtaaaagga aaagaaggtg
     2821 ccatgatgga ttttttatgt gcatttgtcg gcgcaagaga aacaacagag aaatattttg
     2881 attcggcatt tactccaaaa ttcagagcca tgctggctgc ctacgcacaa gcttgcaacg
     2941 attttgcagc aaaaaatccg gataaagtct ggataaaaga tgcttttccg gttaatgaaa
     3001 aagatattat taccggttat gtattaggtt ttgcattgat gaccaacgta ccatattctg
     3061 tcatgaaaat ttccgatggc acaataaaaa cagataatct tttggcacct aaaggcagca
     3121 atggctttgc tctaaacagc aataaaacga tggatggcaa tgtgtatttg ggtatcaact
     3181 ctcatcagcc attagaaggg ccttattcgt ggtatgaagc acatgttcat acagacgaag
     3241 gctgggatat gctgggggca acttttccgg gtggagttac tttatttcac ggagctaatc
     3301 agcatttagg atgggcgcac accgtttcgt ttgctgacca cgatgatgtc tatttattaa
     3361 cgaccagaaa acaagatgga aaaatccagt ataagtatga tggacaatgg caggaattga
     3421 aagtgaaaaa agtaaaatta aaagtaaagt tattttggtt tataaaaatt cctgttacta
     3481 aaaaatatta tgaatctgtt tatggtcctg tcctggaaca cgatggaaaa tattttgcag
     3541 tccgtttccc gtcagcattt gatattcgtg cagctgaaca atggtatcgg atgaataaag
     3601 caaaaaattt ttctgaattt actgatgcac tgaaaatgat ggcctttcca gggcttaatg
     3661 tcatttatgc cgataaagag aataatatta tgtatgccga tatgggacaa ttcccctata
     3721 aaaacagaag ctacgattgg tggcatacct tgcccggaga tacatctgca actttttgga
     3781 aagctaataa ttactggcct tatgacagtt tgattaagat aattaatcct gcctgcggct
     3841 atgtgcataa taataataat acaccatttt attgtactgc ctttgatgaa aactgtgtga
     3901 aacattatca tccgctcgaa aaagattatt tctttttaga aaataacaga agcatacgaa
     3961 cacaatatct gtttgcacat tcaggaaaat tgtcgtatga tgattttaag aaaataaaat
     4021 acgatgtgtc ttttatgaat cccgcctata attatgcaat ggaaaatatt gaagaccttc
     4081 tgcatttatc tcccgaaaaa tatccacaga taaaacaaac tattgcagtt ttgagtaaat
     4141 ggaatcgcag tgcagatgtt gataataagc aggcagcact cgttgcattt gttataaaaa
     4201 aagtaattga tcagctgatt aaagatggca attttcctgc cgtacaagct agagtaaaag
     4261 agccttttct ggcaaaatgt gttgaagatg cacaacacca ttttctcaaa tacttcggaa
     4321 aacttgagat tcctttgggc gattttcaaa aactggtaag aggaaataaa gcattgcctg
     4381 tagccggcgt tccagatgtt attgcagcta tgtatgtaaa agaatatcag gatggtatgt
     4441 ataaagctga agtaggagag acgtatatag aattagtgca gtttacaaaa aacggaccga
     4501 ttatcgaaag tatttcacct tacggtgcga gcaatgtgga aggaaataaa cattatgacg
     4561 atcagatgga tttgtttgtg aaacaacaat gcaagcccat gtctatggaa aaaaaaatca
     4621 tcctgcaaac cgccgaaaaa gtatatcatc ccaatatgtg attttatata tgtattagtt
     4681 tttatgactt tcgtcatgtt ctctgtaaat atgggcattt cggcctcttg ttgtgcataa
     4741 atttttgtca aatcacttta attgtatgta taaactttta actttgcatc gaattttaca
     4801 aatcgtttta tggcaaatag taatcttttc acatcttctg tcggcagaaa aattatagta
     4861 gggcttacag gtttattctt aatcaccttt ttagtagtac atctaggttg taatttgact
     4921 attattatgg atagcagcgg aaatatgttt aatgatgttg cttatttcat gtctcatttt
     4981 tggctgatgc gcatcatcga agtaggatta ttccttgggc ttattgcaca tattgttatg
     5041 acattgaaac taaactttga aaataaaagc aaacgtcctg tcggatatgc tgtttcaccg
     5101 ggtaatgtca attctacctg gtattctcgt tctatggcta ttttaggcag cttattactt
     5161 atctttttag taatacattt atatcagttt tggataccta ccaaaaaagc tttatatatt
     5221 acgcacgaag atgaagatgc ctataccatg gttactgagg cgctgagaaa cccgttggtt
     5281 ttactggttt atatcattgc attcatctcc ttgggctacc atttattaca cggtttccaa
     5341 agtgcattcc agtcattggg attgaaccat aaaaagtata cacccattat caaaaaaaca
     5401 ggaacagcat ttgctattat tgtgcctgct ttatttgcat tgattgcttt agtcgttttc
     5461 tttacaaaat aaggtattac gttggtaaat gtttctgaac atatcatttt aaacaaaatt
     5521 tatatcattc gggataaaaa agtgatgata gatcgggatt tagcagaaat gtatggcgta
     5581 gaaactcgtg tattaaacca agcggtaaaa cgaaatagcg aacgatttcc cgaagatttc
     5641 atgtttcggt ttaatgaaca ggattttgaa aattggaaat cacaagttgt gatatccaat
     5701 aaagaaaaaa tgggaattcg caacatgcct tatgcattta cagaacaagg agttgccatg
     5761 ttgtcaagtg tcttaaacag caaacaagcc attcaggtca atattcagat catccggatt
     5821 tttactaaaa tgcgtgaatt gcttttatcg cataaggaag tcttattgaa gttaaatcag
     5881 cttgaaaaga aactgacgca acacgatgat gatattgcag caatctttaa tgtgctgaaa
     5941 caattattaa gcccaaaaaa taaaccaacc caaaaaattg gtttcaaaat aggtaaacaa
     6001 taaatatttt aaaaattaca aattcagtat atgacactaa attctaaaat accggcaggc
     6061 ccaattgata aaaaatggtc agactataaa ggtcatgtaa aattagtcaa tccggctaat
     6121 aaacgtaaac tagaaattat tgtagtgggt accggtctgg caggtgcatc agctgcggca
     6181 tctttaggcg aattaggtta tagagtaaag gcattttgtt ttcaggattc tcctcgcaga
     6241 gcacactcaa tagcagcaca aggagggatt aatgccgcca aaaattacca aaatgacgga
     6301 gactccgtgt atcgcttatt ttatgatacc ataaaaggcg gtgattatcg tgcccgtgaa
     6361 gccaatgttt atcgcctggc agaagtatcc gtaaacatta ttgaccaatg tgtggcgcaa
     6421 ggcgttcctt ttgccagaga atatggtggc tatctgaata atcgttcatt tggtggtacg
     6481 caagtaaaac gtacttttta tgcagccgga caaacaggac aacaattgtt acttggggca
     6541 tatgctgcct tagaacgtca agttgccttg ggaagtgtta agcaatacac ccggcatgaa
     6601 atgttagatg tcgtaataat tgatggtaaa gctcgaggaa ttatcactcg cgatttagta
     6661 accggaaaaa ttgaaagatt ctttggtcat gctgtcgtat tggcgagcgg aggatatggc
     6721 aatgtgtttt atttgtctac aaatgctatg ggatccaatg ttacagcagc ttggaaagca
     6781 catagaaaag gtgcttattt tggtaatccc tgctttacac aaatacatcc tacttgtatt
     6841 ccggttacag gtgatcatca gtcaaaattg acactcatgt cagagtcatt gagaaatgac
     6901 ggacgtattt gggtgcctaa aatgaaagac gatccgagaa aagcaaatga cataccggaa
     6961 gatgagcgtg attattattt agaaagaaga taccccgcat ttggtaatct cgtacctcgt
     7021 gatgtggctt ctcgtgcagc aaaagagcgt tgtgatgcag gatatggcgt gggtactaca
     7081 aaacaagctg tttatttgga cttcagatat aatctgataa ataaatacgg ccgtgctgag
     7141 gcatctaaac atgggataga aaatcccgat gaagctacac tcgttcgttt aggtaaggaa
     7201 gttgtaaaag aagaatacgg taatttattc gatatgtatg aaaaaattac aggcgaaaat
     7261 ccttatgaag tacctatgcg tatatatcct gccgtgcatt acaccatggg tgggctttgg
     7321 gtagattacg aattaatgac aactgtgcag gggctttatg cattgggaga atgtaatttc
     7381 tctgaccatg gcgcaaaccg tttaggtgca tctgccttga tgcaaggttt ggcggatggt
     7441 tattttgttg ttccttacac tatcggaaac tatttggcag atgagattgc cacccaaccg
     7501 atttctattg accatccggc gtttgttgaa gcagaaaatg gcgttcagga gcgcataaat
     7561 aagctgatga atgtaaaagg aagcaaatcc gttgaaagtt ttcataaaag attgggcaga
     7621 atcatgtgga ataagtgtgg catggctaga aatgcacacg atttgcagga agctatcgaa
     7681 gagataaaag ctctgagaaa agaattttgg tcagatgtga aaattccggg tggtgtaaat
     7741 gagattaatc ctgaattgga taaagcaggt cgagttgcag actttttgga attgggtgaa
     7801 ttgatgtgtc tggatgcttt agacagaaat gaaagttgcg gaggtcattt cagagaagaa
     7861 tatcaaactg aagacggaga agcattgcgt gatgatgtgc attataaata cgttgcactt
     7921 tgggattaca aaggcgacag caactggtca ttaagcaaag aagacttagt gtacgaaaat
     7981 attaaaatag ctgcaagaag ttataaataa tgatctaatt aggaaatatg aaaatgagat
     8041 aatgaaaaca catggatgcc aaggagaata taattgttaa aaaatgtgtt gaattctcat
     8101 tagatgtcat tgagtatagt gaagttttgg aaagtaaaag aaaatttatg attgctaatc
     8161 aattattaaa atctgccaca ttcgttggag caaatgtttt tgaagcacag cattctgaaa
     8221 gcaaagcaga ttttattcat gagatgaaaa tagcaatgaa agaagtaaat gaaacaattt
     8281 attggttaat aatttgcgaa caagctaaat catatcctcc tccattaaat ttgaaaataa
     8341 aaatagagga gatatttaaa ataatttcta aaataattgc tacatcaaaa aagaaagaac
     8401 agacattatc taattaacta attcacaaat tacctaatta aaaagaatat ggaacactac
     8461 aatatgaatc tcacactgaa agtgtggcga caaaaaaaca aaaatgacaa aggcggtttt
     8521 gaaacgtata atgtcaaaaa tatttcttca gaaatgtctt tcttagaaat gttcgacgtt
     8581 ttgaatgagc aattagtaaa tgaaaataaa gaaccgatag cattcgacca tgactgcagg
     8641 gaaggtatct gcggtatgtg cagtatgtat atcaatggtc gtcctcacgg tccatgggcc
     8701 ggcactacaa cttgccagtt gcacatgcgt gcttttaaag atggtgatac gataactatt
     8761 gagccttggc gttcccgtgc atttcctgtt ttgaaagatt tgattgtaga tcgtactcct
     8821 tttgagcgca tcatgcaagc cggaggtttc atctctgtaa ataccggtgt aactattgat
     8881 gctaatacga ttcttgttcc taaagatgta gcagattcag cattcgctgc agccgcatgt
     8941 atcggttgcg gtgcatgtgt agcggcatgc aaaaatgctt ctgccatgtt gttcgtatct
     9001 gcaaaagttt cacaatttgc attattaccg caaggtcacc ctgaacgcag acaacgtgta
     9061 ttaaatatga ttgcacaaat ggacaaagaa ggatttggaa gttgcaccaa taccggcgct
     9121 tgtgaagctg aatgtcctaa acaaatttca ttggaaaata ttgcccggtt aaatcgtgaa
     9181 tatttttctg caagcttggc atctgaagaa taattactaa taacctgagt tcgggataag
     9241 cgtttcaatt aaaaaatagt tgatttttta
//
LOCUS       DAOQII010000082         9237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_3967_length_9237_cov_4.673274, whole genome shotgun sequence.
ACCESSION   DAOQII010000082 DAOQII010000000
VERSION     DAOQII010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_9237_cov_4.673274"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLF48_05445"
     CDS             <1..334
                     /locus_tag="PLF48_05445"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNF48804.1"
                     /translation="TVKQPVGSGTAAKKAPVKTASSGSWFKSPAQEGSEIIFDKLNYY
                     IIAAGSILVILGFILMVGGNKDPKVFNEAEIYSFQRITLSPMLIIFGFLLITGGILKR
                     PAIAAEEK"
     gene            344..1135
                     /locus_tag="PLF48_05450"
     CDS             344..1135
                     /locus_tag="PLF48_05450"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNF48805.1"
                     /translation="MSIIHAIILAVVEGFTEFLPISSTAHMKIVDYLLSIPETPFRNM
                     YEIVIQFAAILAVVLIYRRKFFDFKNINFYLKLIVAVIPALIGGVLLKKHIDAALSNL
                     QFIAIVMMAGGVLLLFVDKWFTKNKIKDEQHVDYLTAFKIGCFQVLSIVFPGLSRSAA
                     TIVGGMSLGMTRKAAAEFSFFLAVPTMLAASVKSLYDVAKEGTVSFSTTEVSILLIGC
                     AVSFAVAFFAVKFFIEMLQRNGFRFFGFYRIALGIFILILLLIKQ"
     gene            1151..1831
                     /gene="truB"
                     /locus_tag="PLF48_05455"
     CDS             1151..1831
                     /gene="truB"
                     /locus_tag="PLF48_05455"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNF48806.1"
                     /translation="MNFVEGEILLIDKPLDWTSFDVVNKLKVALKKKYGRLKIGHAGT
                     LDPKATGLLIVCTGKKTKEIESIQNTDKVYTGTFYLGATTPCYDTEQAIDARFDISTI
                     TAQQVYENAHSFLGEQEQFPPAHSAVKINGKRAYDLARAGKEVPVKSKTINIKKFEIT
                     RIEFPVIDFYVECTKGTYIRSLAHDFGKRMDNGAYLAALRREKIGDYDVIDALQIDSI
                     MEIIYSEQ"
     gene            complement(1839..3221)
                     /gene="glmM"
                     /locus_tag="PLF48_05460"
     CDS             complement(1839..3221)
                     /gene="glmM"
                     /locus_tag="PLF48_05460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNF48807.1"
                     /translation="MALIKSISGIRGTIGGKAGDNLTPLDISKFALAYGSIIKNKGGK
                     KVVVGRDARVSGEMVYHLIVGNLISLGLDVVNLGLATTPTVEMAVTGENADGGIIITA
                     SHNPKQWNALKLLNEKGEFISEKTGQDVLRRANSSKFEFVDIDKLGKITEKDYLDEHI
                     AKILKIPFVDVEAIAAKKMKVVVDAVNSVGGIAVPKLLKALGVQEVICIACEPTGRFE
                     HNPEPLPENLGKLCSEVTYNKADLGIAVDPDVDRLALIDEKGIPFGEEYTLVAVADYL
                     LTYKKGSTVSNLSSSKALKDITLKHGCEYFASAVGEVNVVEKMKEVNAVIGGEGNGGI
                     IYPELHYGRDALVGIALFLSHLATSNKSLHGLRSKYPNYVISKNKIQLEQGINLDFIM
                     QKIAAKYKSYPQNTIDGLRIDINDDWVHLRRSNTEPIIRIYAESSTEKIANNIAQKMK
                     DDIIAILKES"
     gene            complement(3351..3893)
                     /locus_tag="PLF48_05465"
     CDS             complement(3351..3893)
                     /locus_tag="PLF48_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48808.1"
                     /translation="MKNQSKNLLIAIALIAFGVLFRVLHLIPNFSPIAAIALFAGARI
                     SNKKLAVIIPLVAMLLGDLLLSKITGYPFLHDTFVFVYASFILIAFLGFTLKNSNSPV
                     RIFGMAVSSSLLFFILTNFGVWLAGNLYPQTLEGLLTCYIKAIPYYQWTFISDVIFTA
                     VLFSLYSILVRKTDVLFQAA"
     gene            complement(3958..4941)
                     /locus_tag="PLF48_05470"
     CDS             complement(3958..4941)
                     /locus_tag="PLF48_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNF48809.1"
                     /translation="MQIFDNILQTIGNTPIVKLNKITAHIPALVGVKVEYGNPGNSIK
                     DRMAVKMIEDAEVDGRLQPGGTIIECTSGNTGMGLAIAACVKGYHCIFTTTDKQSPEK
                     FDILRALGAEVIVCPTSVEPEDERSYYSVAARLAKEIPNSVWMNQYDNLSNRTAHYES
                     TGPEIWRQTDGKITHFVVGIGTGGTITGTSKYLKEQNPAIKSWGIDTYGSILKQFHET
                     GKYDVNEIHSYITEGIGEDFIPKNYDFSLIDYIEKVTDKDAALMQRRLAREEGLFLGN
                     SAGSAVQGLLQLHEKFPLTKDDVVIIVTHDHGSRYVGKFFNDKWMKEKGFL"
     gene            complement(5019..5702)
                     /locus_tag="PLF48_05475"
     CDS             complement(5019..5702)
                     /locus_tag="PLF48_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48810.1"
                     /translation="MFKLKINYCYFFLAIIIIASCARPYRKVNMESIPFVNYKDDAKI
                     RYSIRQGVFYNFKDYYYARKELKNDYNLIAFSIVNKSELPINISELTFMCGASVIMQP
                     TRMEDIYSVLKQKAGLYWLYSAGVAVYPKPAVMTINVGGTPTQINKPDDPKKLIKNGK
                     QFIPIPFGIVFGAANFGIAYRANKKLKSQLELFDLSNKIIQPGDSIKGWLPFRNVANC
                     GDIFISVKE"
     gene            complement(5831..6316)
                     /locus_tag="PLF48_05480"
     CDS             complement(5831..6316)
                     /locus_tag="PLF48_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48811.1"
                     /translation="MFIPYENLPSTAKVWIYASERPFTATEVTEIDNKLHAFVDNWQS
                     HQMPVRGYGKVLENQFIVLLADDSQIAVGGCSIDSSMQVIRDIAAIYQTDLFDRMRVY
                     FKNSAEQIEFCTIQELKTKVQQGEITNETIVFNPAIAVKANLDLNWQAPFSQSQWYKL
                     V"
     gene            complement(6441..7757)
                     /locus_tag="PLF48_05485"
     CDS             complement(6441..7757)
                     /locus_tag="PLF48_05485"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNF48812.1"
                     /translation="MIRRLLLSLLILLLSLQYVVCMPAFERFKNKREKRKQQQIQIPL
                     YKQAAYTLIKDTVYIRQHVNVHEDTLFNSNFKLKKIVSKYDLIPDTTWQNEYAYAVHY
                     DTIFYHDYIPEDPAYGYTGRIKLNKPYKFFRWSDVLNKPRVGMVAGLQGGLYAIANVW
                     WSSAWYSKYDRDKFHFFNDWNEWNGMDKVAHANNCYFESKWTYDLFRWAGVKEKNAIW
                     IGMLTGNMWQLSIEVNDGFQKKWGFSWGDMAMNVSGSLLFGIQQYLWHDQRIQMKISA
                     FPYNYKQYDDPDITARANKLYGTSFTEILLKDYNAMTFWLSVSPGSFIKNPKSKFPKC
                     IQVSFGYGAGGMLGGRKNIWSSKDLGGDETITTIDPADLVDRTNIQRMHRFYLSMDLD
                     WTKLPVKRHWAQGIMKVLNIIKLPFPAIEFNDNKNGSKVAWHWLKF"
     gene            7920..>9237
                     /locus_tag="PLF48_05490"
     CDS             7920..>9237
                     /locus_tag="PLF48_05490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNF48813.1"
                     /translation="MRNFYILLFLFFHVNTYGNVSIISYGSSWKYLDDGSDQGTAWYG
                     TSFNDASWLSGNGQLGYGDGDESTILNAGCTPVITCTPKFITSYFRKTFTIGTTSTFI
                     NYTLNVKRDDGVVIYLDGVEIYRNNMPGGTILFSTLASAACTDDGYTAQTTSLTLAQL
                     PAGTHTFAAEIHQSAVSSSDISFDLELIANENNASVTRGPYLNLATQQSVHIRWRTNI
                     PANSVVNFGTVSGTLTSTVTDNTLVTEHDVFITGLTADTKYYYSIGNSNPSVQTHYSG
                     NKYFFQTLPIKGSIKPFTFWAIGDCGNNSSNQINVRNAFMNYMGTKTADGMLLLGDNA
                     YNSGYDAEYTTNFFPQYQDSVLRNVLLWPAPGNHDYANNATRQNDHAIPYYDMFTLPA
                     AGEAGGIASGTEAFYSFDYANVHFIALDSYGKESNAYRIWDTLGPQA"
BASE COUNT         2862 a         1778 c         1632 g         2965 t
ORIGIN      
        1 tacagtaaaa caacccgtag gaagtgggac ggcagcaaaa aaagcacctg tgaaaacggc
       61 ttcatccggc agctggttta aaagtcctgc acaggaaggt tctgaaatca tctttgataa
      121 attaaactac tacattattg cagccggttc tatattagtg attcttggtt ttattttgat
      181 ggtaggcggt aataaagatc cgaaagtatt taatgaagca gaaatttatt catttcagcg
      241 catcacctta tctcccatgc tgatcatttt cggcttttta ctgattacag gcggaatact
      301 aaaacgcccc gcaattgctg cagaagaaaa ataagattta cttatgagta ttatacatgc
      361 cattatcctt gccgtggtag agggttttac tgagtttttg cctatatcct caacggcaca
      421 catgaaaatt gttgattatc tgctttccat accagaaaca ccgtttcgga atatgtatga
      481 gatcgtaatt caatttgcag ccatactggc tgtcgtttta atttaccgac gcaaattttt
      541 tgattttaaa aatatcaatt tttatctaaa gctgatagtt gcggtaatac ctgcattgat
      601 aggcggtgtg ttgctaaaaa aacatattga tgctgctctt agtaacttgc aatttatcgc
      661 tatcgtaatg atggcaggtg gcgttttatt gttgtttgta gacaaatggt ttaccaaaaa
      721 caagataaaa gacgagcaac atgtcgacta tctgacggct tttaaaatag gttgtttcca
      781 ggtattgagc atagtttttc cgggcttaag ccgaagtgca gcaacaatag taggtggaat
      841 gagtttgggc atgacaagaa aagctgcggc tgaattttca ttttttctgg ctgttccgac
      901 gatgttggct gccagtgtaa aaagtttgta cgatgtagca aaagaaggca ctgtgagctt
      961 ttcgactact gaagttagca tcttgctgat tggctgtgcg gtttcttttg ctgttgcttt
     1021 ttttgctgtc aaatttttca tagaaatgct gcaacgcaat ggttttcgat tttttggttt
     1081 ttaccgaatt gcactcggta tctttatttt gattttatta cttattaaac agtaattcaa
     1141 tacatttttc atgaacttcg ttgaagggga aatcctgtta attgacaagc cacttgactg
     1201 gacgagtttt gatgtagtga acaaattgaa agttgctttg aaaaaaaaat acggcaggct
     1261 gaaaattggt catgccggaa cattagatcc taaagctacc ggacttttga ttgtttgtac
     1321 cggtaaaaaa acgaaagaaa tagaatctat tcaaaataca gacaaagtat acaccggtac
     1381 attttatttg ggtgccacta ctccctgtta cgatactgaa caagccatag atgcccgttt
     1441 tgatatttca acaattacag cacaacaagt ttatgaaaat gcgcacagtt ttttaggtga
     1501 gcaggaacaa ttcccacctg cacattctgc agttaaaatt aacgggaaac gcgcttatga
     1561 tttagccaga gccggcaaag aagtaccggt taaatctaaa acgatcaaca taaagaaatt
     1621 tgagattaca agaattgaat ttccggtaat tgatttttat gtggaatgta ctaaaggtac
     1681 ttacatccgt agtctggcac atgattttgg caaacgaatg gataacggtg cctatctggc
     1741 ggcattacgt cgcgaaaaaa ttggcgatta tgatgttata gatgctctgc aaatcgacag
     1801 catcatggaa attatttact ctgaacaata aatactgatt agctttcttt caatattgca
     1861 atgatatcgt ctttcatttt ttgtgcgata ttattggcaa ttttttctgt ggaactttca
     1921 gcataaatgc ggattatcgg ttctgtgttg gaacgacgta aatgcaccca atcatcattt
     1981 atatcaatac gcaaaccatc aatagtattt tgtggatagc ttttatattt tgcggcaatc
     2041 ttctgcataa taaaatccag atttataccc tgctccaact gtattttatt cttagatatc
     2101 acataattgg gatatttgct gcgcaagccg tgtaggctct tgtttgaggt tgccaaatga
     2161 gataaaaata gtgctatacc caccagtgca tctctgccat aatgcaattc cgggtaaata
     2221 atgccgccat tgccttcgcc tccgataacg gcatttactt ctttcatttt ctccaccaca
     2281 ttcacctctc caactgcaga agcaaaatac tcgcaaccat gtttaagcgt aatatctttc
     2341 aatgcttttg aagatgataa attggacacc gtgctgcctt ttttgtaggt caataagtaa
     2401 tcggcaacag ccaccaaggt atattcttca ccaaatggaa ttcccttttc atcaatgagt
     2461 gccaagcggt caacatccgg atcaacagct atacctaaat cagctttgtt gtatgttact
     2521 tctgaacata gcttacctaa gttttcaggc agcggttccg gattatgttc aaaccttcct
     2581 gttggttcac aagctataca aataacctct tgaacaccta atgcttttaa taatttaggg
     2641 acggctattc cacctacact atttactgca tctactacta ctttcatttt ttttgcagca
     2701 atagcttcaa catctacaaa aggaattttc agaatcttag cgatatgctc atccagataa
     2761 tccttctctg ttatttttcc caacttatcg atatctacaa actcaaactt ggaggaatta
     2821 gctcttcgta aaacatcttg tccggttttt tcggaaataa actctccttt ttcattcagt
     2881 aatttcagag cattccattg tttcgggtta tgtgatgccg tgatgataat gccaccgtct
     2941 gcattttcgc cggttaccgc catttctacg gtaggtgtgg tagccaatcc gaggtttact
     3001 acatctaaac ccaaagagat taaattacct acaatgaggt gatagaccat ctcgccggaa
     3061 acgcgggcat ctctgccaac tactactttt tttccgcctt tattttttat aatagagccg
     3121 taagctaaag caaatttgga aatatctagt ggggtcaggt tgtcacctgc tttgccgcct
     3181 atcgtacctc tgataccgga tatggatttt atgagagcca atgatttttg ttgttttttg
     3241 tgaattaagc aaatgtagag atatattggc aagaagcgac gtaatgcttc tgctaaaaat
     3301 gtgaaataac tttaaattaa aaattaaata tgaaaaggat aaagcaattt ttatgcagcc
     3361 tggaaaagta catctgtttt gcgaaccaga attgaataca aggaaaacaa aacagccgta
     3421 aaaatcacat cactgataaa tgtccactga tagtaaggaa tagctttaat gtaacaagtc
     3481 aataaaccct ctaatgtttg gggatagaga ttgcctgcca accatacgcc aaaatttgtc
     3541 aggataaaaa acagcagtga agaactaaca gccatgccga aaatacggac tggactattg
     3601 ctgtttttca acgtgaatcc taaaaatgca atgagtataa aagaggcata aacaaacacg
     3661 aaagtatcgt gcaggaatgg ataacctgtt atttttgaca acagcaaatc acccagcaac
     3721 attgccacaa gcggaattat aactgctaat tttttgttag aaattcttgc ccctgcaaat
     3781 aatgcaatgg ctgctattgg gctaaaattg ggtatcaaat gtaagactct gaacaagaca
     3841 ccaaatgcta ttaaagctat tgcgataagt aaatttttag actgattttt catatctcaa
     3901 atataaaaca aaatttttgt gttgtgtaaa aagctttctt aaatgatgtt agtagattta
     3961 taaaaatccc ttttctttca tccacttatc gttaaaaaac ttgccgacat aacgtgaacc
     4021 gtgatcgtgc gttacaataa tcactacatc atcttttgtc aatggaaatt tttcatgcaa
     4081 ttgcaataaa ccttgtacgg cagatcctgc agaattaccc aaaaacaaac cttcttcacg
     4141 ggctaagcgg cgctgcatca atgctgcatc tttatccgtt actttttcga tataatctat
     4201 taagctgaaa tcataatttt ttgggataaa atcttcgcca ataccttctg taatataact
     4261 gtgaatttca tttacatcat atttacccgt ttcatgaaat tgtttcaata tagaaccata
     4321 ggtgtctatt ccccaagatt tgatggcagg attttgttct tttaaatatt tgcttgtgcc
     4381 tgtaatagta ccaccggtac caatgcctac aacaaaatgt gtaatttttc cgtctgtttg
     4441 tcgccaaatt tccggacctg tactctcata atgtgcagtt cggttagaca aattatcgta
     4501 ttggttcatc cagacagagt tgggaatttc ttttgccaaa cgggctgcca ctgaataata
     4561 agaacgctca tcttctggtt ctacgcttgt agggcaaaca attacttcag cccctaatgc
     4621 tcggagaata tcaaattttt caggcgattg tttatcggta gtagtaaata tacaatgata
     4681 accctttaca catgcagcaa tagccaagcc cattcctgta tttccactgg tacattcaat
     4741 aatcgtacct ccgggttgca gtctgccatc tacttcagca tcttcaatca tctttaccgc
     4801 catgcggtct ttgatagaat tccccggatt accgtattct actttcaccc ctaccaatgc
     4861 gggaatatga gctgtaattt tattcagttt aacgattggc gtattgccga tagtttgcaa
     4921 gatattgtcg aaaatttgca tgggctaaag atacaaaaat tttgaaaggc ttatgacaag
     4981 cataaaaagc agtaaggatt aaatctaaaa aacaagaatt attcttttac tgaaataaat
     5041 atatccccgc aattagcaac atttctaaac ggcaaccatc cttttatact atctcccggt
     5101 tgaatgattt tgttggagag gtcgaaaagt tcaagctggg attttaattt tttattggca
     5161 cgataagcaa ttccgaagtt tgctgcaccg aaaacaatac caaatggaat cggaataaat
     5221 tgttttccgt ttttgattaa ttttttagga tcgtcaggtt tgttaatttg tgtaggtgta
     5281 ccaccaacat taatggtcat aactgcaggc ttagggtata ctgcaactcc tgcactataa
     5341 agccagtaca acccagcttt ttgcttaagt acactgtata tatcttccat tcgtgttggc
     5401 tgcataatta ccgaagcacc gcacatgaac gttaactcac tgatattaat cgggagttca
     5461 gatttattga caatactgaa tgcaatcaaa ttataatcat tcttcaattc ttttcgggca
     5521 taatagtaat ctttgaaatt atagaatacc ccctgtcgaa tggagtagcg gatttttgca
     5581 tcgtctttat aattaacaaa agggatactt tccatattta cttttctgta tggacgtgca
     5641 catgatgcaa taataattat tgccaaaaaa aaatagcaat agttgatttt aagtttaaac
     5701 atattttagt agttagaagt tgtatttagc atgataaaaa taaacaatta ttcgacgaaa
     5761 tacaagaaca agaatttatc aaattttatt ttttgacaaa tcaaaaattc atgaagcaag
     5821 atgagataaa ctatactaac ttataccatt gcgactgact aaatggtgcc tgccagttaa
     5881 gatcgagatt ggctttaaca gcaatagccg gattaaatac aatcgtttcg ttcgtaattt
     5941 caccttgttg tacttttgtc ttcaactctt gaatggtaca aaattctatt tgttcggcac
     6001 tattcttaaa atacacacgc attctgtcaa ataaatcagt ttggtaaata gctgcaatat
     6061 cgcgaataac ctgcatagag ctgtcaatgg aacagccgcc tactgcaatt tgggaatcat
     6121 cagctaataa gacaataaat tgattttcca acactttacc atatcccctt acaggcattt
     6181 gatgcgattg ccagttatct acaaaagcat gaagtttgtt gtcaatttcc gtaacttctg
     6241 ttgcagtgaa cgggcgctct gaagcataga tccagacttt ggctgtggat ggcaaatttt
     6301 cataaggaat aaacatatct cttacacttt atcaatttct aaatctattt ttttttacaa
     6361 agttaaaaat gttttgttcc ctgccggatt ttttttgaaa gctataatta tataataaca
     6421 tggttgaaaa ttattctaat ttaaaacttc aaccaatgcc aggcgacttt gctaccattt
     6481 ttattatcat taaattcgat tgcagggaaa gggagcttaa taatattcag aactttcatg
     6541 ataccttgag cccaatggcg ttttacaggc aattttgtcc agtccagatc catcgataaa
     6601 tagaaacgat gcattcgctg aatattcgtt ctgtccacca aatccgcagg gtcaatggtt
     6661 gtaatggttt catcaccacc taaatcttta ctcgaccaaa tattttttct acctcccagc
     6721 atgccaccgg caccataacc aaatgaaact tgtatgcact tgggaaattt tgatttagga
     6781 tttttaataa aagaccccgg actaacactc aaccaaaacg tcatggcatt ataatctttc
     6841 agcagaattt cggtaaaaga tgtgccataa agcttattgg ctcttgccgt aatatcagga
     6901 tcatcatatt gcttgtagtt gtaaggaaaa gctgaaattt tcatttgaat tctttgatca
     6961 tgccataaat attgctgtat gccaaacaat aaacttccac tgacattcat ggccatatca
     7021 ccccatgaaa aaccccattt tttttgaaaa ccgtcgttca cctcaataga taattgccac
     7081 atgttgccgg tgagcatgcc aatccagatt gcattttttt cttttacacc tgcccatctg
     7141 aataagtcat acgtccattt agattcaaaa taacaattat tggcatgggc aactttatcc
     7201 atgccgttcc attcgttcca gtcattgaaa aaatggaatt tatctctatc gtattttgaa
     7261 taccaggcac tgctccacca tacatttgca atagcataca aaccaccttg caaacctgcc
     7321 accataccta cacgaggttt attcaacaca tcagaccagc gaaaaaactt gtaaggttta
     7381 ttgagtttta ttctacctgt atagccataa gcggggtctt cggggatgta atcgtgataa
     7441 aatatcgtgt cgtagtgtac ggcataagca tattcgtttt gccaggtagt atccggaatt
     7501 aaatcatact tgctgactat cttttttagt ttaaaattac tgttgaataa agtgtcttcg
     7561 tgaacattca catgttgacg aatatataca gtatctttaa tcagggtgta agccgcttgt
     7621 ttatacaagg gaatttgaat ctgttgttgt ttacgctttt ctcttttatt tttaaatctt
     7681 tcaaatgcag gcatacaaac cacatattgt aagctgagca acaaaatcaa tagggataat
     7741 aataatcttc gaatcaaatt ttaaaaattg tacggtaaaa taaaaaacta gacttacaca
     7801 ttcaagaaaa atgcagaatt ctattcctaa ataataaaaa ttaatggaaa tactttcatt
     7861 taaatataat agaatcaagc gaatgcgaca aaaaagtgat aattttatac cacataccga
     7921 tgcgcaattt ttacatactg ctatttttgt tttttcatgt taatacatac ggcaatgtaa
     7981 gcattatctc atatggttca tcatggaaat atctggatga tggcagtgat cagggaacag
     8041 catggtatgg tacttctttt aatgatgcct cttggttaag cggaaatggt cagctgggtt
     8101 atggagatgg tgatgaatcg acgatactaa atgcaggctg cacaccagta atcacatgca
     8161 cacctaagtt tatcacttct tatttcagaa aaacatttac aataggcact acctccacat
     8221 ttattaatta caccctaaat gtaaaaagag atgacggtgt ggtcatctat ttggatggtg
     8281 tagagattta cagaaataat atgcccggtg gcactatctt gttcagcaca ttagcctctg
     8341 ctgcatgtac ggatgatgga tacaccgcac aaacaacatc gcttactttg gcacaactgc
     8401 cagccggaac gcatactttc gcagccgaaa ttcatcaaag tgctgtttca tcttctgata
     8461 tttcctttga tttggaatta attgcaaatg aaaacaatgc cagtgtaacc agaggtccct
     8521 atctcaattt agccactcag caatcggtac atataagatg gcgaactaat attcctgcaa
     8581 attctgtcgt aaattttggc acagtttccg gcacgctaac ttccactgta acagataata
     8641 cattagttac ggaacatgat gttttcatca ccggattaac tgcagatacg aagtattatt
     8701 attcaatcgg aaattcaaat ccgtctgttc agacacatta ttccggcaat aagtatttct
     8761 ttcaaacact gcccattaaa ggcagcataa agcccttcac tttttgggca attggtgatt
     8821 gtggcaataa ctcttccaat cagataaatg tgcgcaatgc atttatgaat tatatgggca
     8881 ctaaaacggc agatggtatg ttgttgctcg gtgataatgc atataattcg ggatatgatg
     8941 ctgaatacac gactaacttc tttccgcaat atcaggattc ggttttgcgc aatgtcctat
     9001 tatggcctgc acccggcaat catgactatg ccaacaatgc tacccgtcaa aatgaccatg
     9061 ctattccata ttatgacatg tttacattgc ctgccgccgg cgaagccggc ggcatagctt
     9121 ccggcactga ggcgttctat tcttttgatt atgccaatgt tcattttata gctttggatt
     9181 cttatggaaa agaatcaaat gcttaccgaa tttgggatac tttaggtcct caagcat
//
LOCUS       DAOQII010000083         9184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4008_length_9184_cov_5.201117, whole genome shotgun sequence.
ACCESSION   DAOQII010000083 DAOQII010000000
VERSION     DAOQII010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4008_length_9184_cov_5.201117"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1378
                     /gene="accC"
                     /locus_tag="PLF48_05495"
     CDS             35..1378
                     /gene="accC"
                     /locus_tag="PLF48_05495"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNF48814.1"
                     /translation="MFKKILIANRGEIALRVIRTCREMGIKTVAVFSTADKDSMHVKQ
                     ADEAFCIGPPASKDSYLKKEQIIEAALLTDADAIHPGYGFLAENEDFARLCTENKIKF
                     IGPTPEQIAAMGDKITAKETMQRAKVPVIPGSEGLITDIAEGKKLASIIGYPVILKAT
                     AGGGGKGMRIVGHEEEFESNLMQAQAEAGSAFGNAGMYLEKYIEEPRHIEVQVAGDQF
                     GNAAHFSERDCSIQRRHQKLVEESPSPFVDEALRKKLGEAAVNAVKAIKYEGVGTIEF
                     LVDKHKKFYFMEMNTRIQVEHPVTEEVVGVDLIRLQIELAAGKKLEKEAWLPTGCAME
                     CRINAENPYDNFKPSPGKITNLQLSGGYGVRIDSHIYAGYSIPPFYDSMIAKLIVHGQ
                     TREEVIQRMKRALLEMNIEGIKTTIPFHLQLMEHADFKSGNFTTKFLEDFEMEEE"
     gene            complement(1450..2724)
                     /locus_tag="PLF48_05500"
     CDS             complement(1450..2724)
                     /locus_tag="PLF48_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNF48815.1"
                     /translation="MTQETVVTLILILICLIFAALFSGIEIAFLSANKLKIELSNQRG
                     DLSGRILNDYIKNPSKFIVTTLLGNNIVLIAFGILSAGLLEPLIERFTHNHILVILIQ
                     TIISTSIVLLFGEYIPKTLFKVFADSILPVLAIPFKVIHWLLTPGVLLINGLSTLLFK
                     IFGFKITEHDIQFSSLDLERFIKEHSTGTDEDEEEVDTELFENALHLKNLRIRDCMVP
                     RREITAIDVNDMIDDLIQLVIETNHSRIIVYDDNIDTIVGYIHHFDLHQQPSDIRSIL
                     MPIKIVPETLPIQKLMNDFIKEHKSIAWVVNEYGGTAGIITLEDILEEIFGEIEDEYD
                     KDELIDNQLSKNEFILSGRLEIDRINEEYDLHIPEGDYDTLSGFIISHHETIPEKQEI
                     IEIDQYTFKILDVSETKIETVKLIIKDETKDE"
     gene            2842..4689
                     /locus_tag="PLF48_05505"
     CDS             2842..4689
                     /locus_tag="PLF48_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48816.1"
                     /translation="MKKQFLVLGLFWLFFINAFAELNILEKSYDITRKARKGYLGAVE
                     TNKEKNTFDLIYILKSNNRKIITETYTYDKDLNLVNTVKDEQDVEKVRSRYKWFRYRG
                     EEYSVNSIYVRSNAKGEMIFRQKTILYKWSWWRGGYAKKIKLGDKIKPVDEESGARYM
                     FRGGYYENDNSGKLVVVAGLKDGKDNISSYKNYQVLEIDSLANIKVKADIKFNAAYAP
                     VFSDIVDDDDPTSQDESPRDWIVVFAPQGGKSFKGATGTANDFIYVRMSPDGSIKEQY
                     NFTAPVAGWRILGATEKNGQVYLYGPSISKDDRFINEVFKGQMLANTTNEDAHEDNAT
                     NNTGGFFGGGLGSFGKSIKNMATGEAFQVTQDDIDKRMDEMKYTNVQVAAFNSGKLQF
                     ISSPTIDEINANLIVPTGQKKAIEFDGKRFITTGIYVTNNGDIFICGQDYKLDGWGKD
                     NKGSRLYKAMFMVQFNAQGKYLRNYGLKLDQKKYMGGFTKGVTPDMYPAYSYLEESVD
                     GKKLYWMLNICKAIDKDVDYDSDYNFFSGVKTTTTTSTFTPLNSIEYGTIDVATGATS
                     DFKTLGEDENRKFYLMDNVNKVRLNPYLIYLSETTKGDKILLSRFDISQ"
     gene            4808..5188
                     /locus_tag="PLF48_05510"
     CDS             4808..5188
                     /locus_tag="PLF48_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48817.1"
                     /translation="MKEKKEIPLNSGRSCLLIKIKNDFIPNYYLIAFPKTQGEPTPEE
                     IAEMMKIGIEYAQRLSLEFIGDKEAFSVLYSGYSSRREKGWHIHIILLGNRWKKAWLY
                     LVLASKNITQALHLRKDDAPKNKD"
     gene            5253..6344
                     /locus_tag="PLF48_05515"
     CDS             5253..6344
                     /locus_tag="PLF48_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF48818.1"
                     /translation="MKSKSFKLTIENPCLENWEGMSSIENGRYCNTCKMKVIDFTNST
                     KSDIEKFFAQNRTGHCGRFKKSQLEQVYHYPNEDLIKRNKFKYAAAITFGLLTAKSAS
                     AQIKNENTEIIHIDQADSYLSKQLTTTKDSTNLTIRIKSAADLIVIPNCRLLIFSITK
                     GYTFVAKTDSLGLYSCSVPLEDFPFQVYINANGFHIDSISIHDINSKNEILLNPIINE
                     EILMGAPIHSNSFRDIYFDFAQISIRSEGEKTLKNILAFLKENTDVNLEINAYTDSRG
                     SSKSNLNLSKQRAEEIKKWFTNKGINKKRIIAKGYGETEFVNDCKDGKECTEYEHQQN
                     RRVEFRLIGKDIDINSVKRFDMKVSPYRK"
     gene            complement(6412..7434)
                     /locus_tag="PLF48_05520"
     CDS             complement(6412..7434)
                     /locus_tag="PLF48_05520"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNF48819.1"
                     /translation="MKNLTFALCLSLLVISCKETAIQEPLPPSKIAFGSCCIQVGDLT
                     IFDSILKMQPQLYLALGDNMYGDAIAGEPDKAWLELNYTILGNNASFKRLKSNVPMIA
                     TWDDHDFGGNNRGSNYTQKQDSKDAFLSFFNEPAESERRSHPGIYTSYFYGDEAHRLQ
                     IIVLDTRWFLDVFSGEPISPTSDTSKHFLGQVEWDWLKAELLKPAKVRIIMTSTQMLH
                     EHNGYESWTNYPHELNRFFQLLKETRANGVFMVSGDVHYAELSKRSMPDMYPLYDMTS
                     SGLNQVLDNPQPNMYRVGNGLKELNFGMINIDWNSNPISISLEVYNKKRQLKIQQTVT
                     TDELKF"
     gene            7644..>9184
                     /locus_tag="PLF48_05525"
     CDS             7644..>9184
                     /locus_tag="PLF48_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF48820.1"
                     /translation="MKILIQYLMRYRNLVVLMFVLAIINQGFSLIDPMIIGKIISQYG
                     LKASQYTQSQFIKGVLSLILVSIGVAMISRIAKAFQDYYMNLIIQKFGVDVYTDGLKH
                     ALLLPFQEFEDRRSGETLNILQKVRTDTEKFIQYFINVVLFAIISIAFIIFYSISVYP
                     KLILMYFLASAILYVVMQQLSKKIKEVQTAIVKKTNQLAGATTESLRNIELIKSLGLA
                     QQEVTRLNNNSFQILNLELEKVKKVRSLTFIQGTLVNTLRQVIVFLLMYLIFKDQLNV
                     GQLYTLQIYSFFIFNPLQEMGNVILSYREAQASLHNFNKLLSTPIETKPKHPEHLHAI
                     QTLAFQDVSFQHKSSKTKAVEDINFSVKKGDSIAFVGPSGSGKTTLVKLLVGLYQPQT
                     GHILYNNIDSGKIDIDELRKQIGFVTQETQLFAGTIKENMLFVQPDASDADIIDALNK
                     ASCQSLLARSDKGIDSVIGEGGMKISGGEKQRLSVARALLRHPNLLVFDEATSALDSL
                     TEEEIT"
BASE COUNT         3101 a         1633 c         1726 g         2724 t
ORIGIN      
        1 ctaattagct aattatttaa ttaactaatt tagcatgttc aaaaaaatcc tcatagccaa
       61 ccgtggtgaa atcgccctcc gtgttattcg tacttgccgt gaaatgggca taaagaccgt
      121 agcagttttt tctacagcag ataaagacag tatgcatgta aaacaggcag atgaagcttt
      181 ttgcataggc cctcctgcca gtaaagattc ctatctgaaa aaagagcaga ttatagaagc
      241 tgctttactt accgatgcag atgccataca tcccggctat ggttttctag cagaaaatga
      301 agatttcgca cgactatgta cagaaaacaa gataaaattt atcggcccta cacctgagca
      361 gattgctgcc atgggcgata aaataaccgc aaaagaaact atgcaaagag ccaaagtgcc
      421 cgtcattccg ggatctgagg gcttaattac agatattgcg gaaggcaaaa agttggcaag
      481 tattatcggt tatcctgtaa tattgaaagc aacggccgga ggcggaggca agggcatgcg
      541 catcgtggga catgaagaag aatttgaatc taacctgatg caggcgcagg cagaagcagg
      601 cagtgcgttc ggcaatgcag ggatgtattt agaaaaatat atagaagaac ccagacatat
      661 tgaagttcag gtggccggcg accagtttgg aaatgccgcc catttttcag aacgcgattg
      721 ctccatacaa cgccgacatc agaaattagt ggaagaaagt ccgagtccgt ttgtggatga
      781 agccctgaga aagaaattag gtgaagctgc agtaaatgca gtaaaagcca taaaatatga
      841 gggtgtaggt accatagaat ttttggttga caaacacaaa aaattttatt ttatggagat
      901 gaatacccgc atacaggtag aacatccggt aacggaggaa gtggtaggtg tggatctgat
      961 tcgtttgcag atagagctgg cagcaggaaa aaaattggaa aaagaagcct ggttgccaac
     1021 agggtgcgcc atggaatgtc gtataaatgc cgagaatcct tatgacaact tcaagccatc
     1081 acccggaaaa atcaccaatc tgcaactcag tggcggctat ggcgtgcgta tcgattcgca
     1141 tatttatgcc ggttattcca ttcctccatt ttatgactca atgattgcca agctaattgt
     1201 acatggacaa acaagagaag aagttattca acgtatgaaa cgagcattgc ttgaaatgaa
     1261 tatagagggt ataaaaacaa cgattccctt tcatcttcaa ctaatggaac atgcggattt
     1321 caaaagtggc aatttcacca caaagtttct ggaagatttt gaaatggaag aagagtagga
     1381 atttgttatt ttgaaaatca actgattcga aaatactggc tatagctagc atggaagaat
     1441 ctcaactaat tattcgtctt tggtctcgtc tttgataatc agtttcacgg tttcaatttt
     1501 ggtttctgaa acatccaaaa tcttaaaagt gtattgatct atttctatta tctcctgctt
     1561 ttcaggtata gtttcatgat gtgatataat gaagcctgaa agggtatcat aatcgccttc
     1621 gggaatatgc aaatcatatt cctcattgat gcggtctatt tccagccttc cggataaaat
     1681 aaactcattt tttgaaagtt ggttatcaat caattcgtct ttgtcatatt catcttctat
     1741 ttctccgaaa atttcttcta aaatatcctc cagcgtaata atgcctgccg tacccccgta
     1801 ttcatttacc acccaagcaa tgcttttatg ttctttaata aagtcattca tgagtttctg
     1861 aatgggcaat gtttccggaa ctatttttat cggcatcaag attgaacgaa tatctgacgg
     1921 ttgctgatgc aggtcgaaat gatgaatata gcccactatc gtgtctatat tgtcatcata
     1981 tactattatt cgtgaatgat tcgtttcaat aaccagctgt atcaaatcat caatcatatc
     2041 attgacatca attgccgtaa tttcccttcg cggcaccatg caatcacgaa tacgcaaatt
     2101 tttgagatgc aatgcgtttt caaataattc cgtatctact tcttcctcat cttcgtccgt
     2161 gccggtagaa tgttctttta taaatcgttc caaatccaag gatgaaaatt gtatatcgtg
     2221 ttcggtaatt ttaaacccga aaatcttaaa cagtaaagta gataaaccat ttatcagcaa
     2281 cacgcccggc gtcagcaacc agtgaattac tttaaacgga attgccaaaa caggtaaaat
     2341 actgtctgca aaaaccttaa aaagagtttt agggatatac tcaccaaaca acaaaacaat
     2401 agaagtagaa atgattgtct gaataagaat aaccagaata tggttatgag taaaacgttc
     2461 tatgagtggc tctaataagc ctgccgataa aataccaaat gcaatcaaca caatattatt
     2521 acccaaaagt gtggttacaa taaattttga cggattcttg atataatcat ttagtattcg
     2581 cccgcttaaa tcaccccgct gattgcttaa ttctatcttg agtttattgg cggataaaaa
     2641 tgcgatttct attccggaaa ataaggccgc aaaaataagg cagataagaa tcagtataag
     2701 cgtaacgact gtttcctgtg tcatatctga caaaaataga aaagaaaatc ggtattgcgg
     2761 aaaatacgta gtgtgaaata ccacgaatta cggataaccg acctctgtaa ttaggaatag
     2821 ttttacttaa aaataattat tatgaaaaag caattcttag tactcggttt attctggctg
     2881 ttttttatca acgcctttgc agaattaaac attcttgaga agtcgtatga catcacccgc
     2941 aaagcaagaa aaggatactt aggtgcagta gaaaccaaca aagagaaaaa tacatttgac
     3001 ttgatatata tcctcaagtc gaacaacaga aaaatcatta cagaaaccta tacctacgac
     3061 aaagacctga atttggtgaa taccgttaaa gacgaacaag atgtagaaaa agttcgttcg
     3121 cgttacaagt ggtttcgtta tcgcggggaa gaatattccg ttaacagcat ttacgttcgt
     3181 tccaatgcga aaggtgaaat gatatttcgt cagaaaacca tcctttacaa atggtcgtgg
     3241 tggagaggcg gttatgccaa aaaaataaaa ttaggggata aaattaaacc cgttgatgaa
     3301 gaaagtggag cacgttatat gtttagaggt ggctattatg aaaatgataa ttcaggtaaa
     3361 ctcgttgtgg tggcgggttt gaaagacgga aaagacaata tttcttctta caaaaattat
     3421 caggtcttag aaattgactc attggcaaat atcaaagtta aagccgacat aaaatttaat
     3481 gctgcctacg cacctgtatt ttctgatatt gtagatgatg atgacccaac atcacaagat
     3541 gaatcgccgc gtgactggat agtcgtattt gcaccgcaag gcggtaaatc attcaaagga
     3601 gctaccggta cagcaaatga ctttatatat gtgcgtatgt caccggatgg cagcatcaaa
     3661 gagcaatata attttacagc tcctgttgca ggttggcgta tattgggtgc taccgagaaa
     3721 aacggacaag tgtatctgta tggtccatct atcagtaaag atgaccgttt tatcaatgag
     3781 gtatttaaag gtcagatgct agccaacacg acaaatgaag atgcgcatga ggataacgca
     3841 acaaataata caggcggttt ctttggcgga ggtctgggtt catttggcaa atcaataaaa
     3901 aatatggcaa ccggtgaagc ttttcaagtt actcaggacg atatagacaa acgcatggac
     3961 gaaatgaaat ataccaatgt tcaggtggca gccttcaata gtggaaaatt acagtttata
     4021 tcctctccta ctattgatga gatcaatgca aatttaatag ttcctacagg acaaaagaaa
     4081 gctattgaat ttgacggcaa aagattcatc accacgggca tctatgtcac caacaacgga
     4141 gatattttta tctgtgggca agattataaa cttgatggct ggggaaaaga caataaaggc
     4201 tcaaggctgt ataaggccat gtttatggtt caatttaatg cacagggaaa gtacttaagg
     4261 aattatgggc ttaaactcga tcagaaaaaa tacatgggtg gttttacaaa aggagtaaca
     4321 cccgatatgt atccggcgta ttcatatttg gaagaatcag ttgacgggaa aaaattgtac
     4381 tggatgctga atatctgcaa agcaattgat aaagatgtag attatgacag tgactataat
     4441 tttttctccg gtgtaaaaac tacgactact acttcaacat tcaccccgct caactctatt
     4501 gagtatggca ccatagatgt ggctacggga gctacgtcag attttaaaac tttgggagaa
     4561 gatgaaaacc gtaaatttta tctgatggat aatgtaaaca aggtgagatt aaatccgtat
     4621 ctcatctatt taagtgagac tacaaaaggc gataagatac tgctttcgcg gttcgatatt
     4681 tcgcagtagt gataattatt tttttcggtg cgggcggggc tgaaagcccc gcccgcattg
     4741 ttttatatta cacaaatatt gaaatattat agattaactt tacaaaaaat caatactttt
     4801 actcttgatg aaagagaaaa aagaaattcc acttaattca ggcagaagct gtttgctcat
     4861 taaaataaag aatgatttta ttccaaatta ttacttaatt gcatttccaa aaacgcaagg
     4921 tgagccgact ccagaagaaa ttgccgaaat gatgaagatt ggaattgagt atgcacagcg
     4981 attatcattg gaatttattg gagataaaga agcattttcc gtattataca gtggctatag
     5041 ttcgagaaga gagaaaggat ggcatataca tattatttta ctaggtaaca gatggaaaaa
     5101 agcatggtta tatttggtat tagcctctaa aaatataact caagcactcc atctcagaaa
     5161 agacgatgct ccaaaaaata aagattaatt atgtagctta aatatatctt tattcatttc
     5221 actttattca tactttaagt tacatttgaa caatgaaatc aaaatctttt aaactaacaa
     5281 tagaaaaccc atgcttagaa aattgggaag ggatgagttc tattgaaaat ggcagatatt
     5341 gcaatacttg caagatgaaa gtgattgatt tcacaaattc aacaaaatct gatattgaaa
     5401 aattctttgc acaaaataga accggacatt gcggtcgttt taagaaatct caattggagc
     5461 aagtgtatca ttatccaaat gaagacttaa taaaaagaaa taaattcaag tatgccgccg
     5521 ctattacatt cggcttatta acagctaaat ctgcatctgc tcaaataaaa aatgaaaata
     5581 ctgaaattat tcatattgat caagcagact cttacttaag taaacaactg acaaccacaa
     5641 aagacagtac aaatttgact attagaataa aatcggcagc tgacctgatt gtaattccaa
     5701 attgcaggct attaatattt tcaataacaa aaggatatac atttgttgca aaaacggata
     5761 gcctcggtct ctattcatgt agtgtaccgc tagaagattt tccttttcaa gtctatataa
     5821 atgctaatgg atttcacatt gattcaattt ctattcatga cattaattct aaaaatgaga
     5881 tattattaaa tccaattata aacgaagaaa ttctaatggg agcaccaatt cattctaatt
     5941 cttttagaga tatttatttt gattttgctc aaatttctat acgctctgaa ggcgaaaaaa
     6001 ccttaaaaaa cattttagca tttttaaagg agaatactga tgtgaattta gaaattaacg
     6061 catatactga cagtagagga agcagtaaat ctaatttaaa cttatctaaa caaagagctg
     6121 aagaaattaa gaaatggttt acaaataaag gtataaataa aaaaagaatt attgcaaaag
     6181 gctacggtga gactgaattc gtaaatgatt gtaaagatgg aaaagaatgt acggaatatg
     6241 aacatcagca aaatagaaga gtagaatttc gtctgatagg aaaagatatt gatattaatt
     6301 cagtaaaaag attcgatatg aaagttagcc cctacagaaa atgagtatta ctatttttca
     6361 aattgtatta atacactttc acagcaacct aaatacatag ttttaagtgg gctaaaactt
     6421 cagctcatct gttgttactg tctgttgtat tttcagctgc cgttttttgt tatacacttc
     6481 cagcgagatg ctaatcgggt tgctattcca atctatattt atcattccga aattaagctc
     6541 tttcagccca ttgcctactc tgtacatatt cggttgtgga ttatccaata cctggttcaa
     6601 gccacttgaa gtcatatcat acagcggata catatccggc atgcttcttt tgctcaattc
     6661 tgcataatgt acatcaccac ttaccataaa aaccccattg gctctggtct ctttcagtaa
     6721 ttggaaaaaa cgattcagtt cgtgcggata atttgtccac gattcataac cgttgtgctc
     6781 atgtagcatt tgtgtactgg tcataatgat gcgcacttta gccggtttta ataattctgc
     6841 tttcagccaa tcccattcca cttgtcctaa aaaatgttta gaggtatctg acgtcggaga
     6901 aataggctct ccactaaata catccagaaa ccagcgtgta tcaagtacga tgatttgcaa
     6961 gcggtgagct tcatcaccat aaaaataaga ggtataaatg cccggatggc ttcttctttc
     7021 tgactcagcc ggttcattga aaaagctcaa aaaagcatct ttagaatcct gtttttgcgt
     7081 atagttactt cctctgttat tgccgccaaa atcgtgatca tcccaagtgg caatcattgg
     7141 tacattactt ttcagtcgtt taaatgaagc attatttccc aaaatggtat aattcaattc
     7201 cagccatgct ttatccggtt ctccggctat ggcatcacca tacatattat ctcccaaagc
     7261 cagatagagc tgaggttgca tcttcaagat agaatcgaaa atagttaaat ctccaacttg
     7321 aatacaacaa gatccaaaag caatttttga aggaggcagt ggctcttgta tagctgtttc
     7381 tttgcatgaa atcactaata acgacaaaca taaagcaaag gttaaatttt tcatattacg
     7441 catttcaaaa ctaaactatg caaattattg cacaataaag aatttcgtat attgtattat
     7501 aatcaatagt attatttaaa acaatattct atatattttt gagtttgtca tttttattgc
     7561 atatctgtta acactctttc ttacctgtaa ccattaaaac tattgtatat acaattctct
     7621 ttaattttcc ttacttttgt gtcgtgaaaa ttttaatcca gtatctgatg cgctaccgca
     7681 atctggtggt gctgatgttt gtattagcca tcatcaacca gggcttctcg ctaatcgacc
     7741 cgatgataat cgggaaaatt atcagtcagt atggcttaaa ggccagccag tatacacaat
     7801 cacaatttat caaaggggta ttatcactta ttttggtttc tatcggcgtg gccatgatca
     7861 gccgtattgc caaggcattt caggactatt atatgaatct catcattcag aaatttggag
     7921 tagatgtata tacggatggg ctgaaacatg ccttgctctt gccctttcag gaatttgaag
     7981 acagacgcag cggggagacc ttgaatattt tacaaaaggt acgtactgat acagagaaat
     8041 ttatacaata ctttatcaat gtagttttgt ttgctattat cagtatagca ttcatcattt
     8101 tttattctat ttcggtttac cccaaactta tcctgatgta ttttcttgcc tctgcaatct
     8161 tgtatgttgt catgcagcaa ctttccaaga aaataaaaga agtacaaaca gctatagtaa
     8221 aaaaaacgaa tcagttggct ggtgctacca cagaaagctt gcgcaatatt gaactgataa
     8281 aaagcttagg acttgctcag caagaggtaa cccgtctaaa caataacagt tttcagattc
     8341 ttaatttaga actggaaaag gtaaaaaaag tgcgcagctt aacatttatt caaggcacac
     8401 tggtcaatac gttacggcag gtcattgtgt ttttattgat gtatctgata tttaaagacc
     8461 aattgaatgt aggacagctt tatactttac agatatactc cttttttatt ttcaaccctt
     8521 tgcaggaaat gggcaatgtc atccttagtt atcgcgaagc tcaggcatca ctgcacaatt
     8581 ttaataagct gttatccacg cctatcgaaa ccaaaccaaa gcatcccgaa catttgcatg
     8641 ccatacaaac acttgccttt caggacgtat cgtttcagca taaatcttca aaaactaaag
     8701 cagtagaaga tattaatttt tctgtaaaaa aaggtgattc cattgcattt gtcgggccat
     8761 cgggttccgg aaaaacaaca ctggtaaaat tgttagtggg cttgtatcaa cctcaaaccg
     8821 ggcatattct ttataataat atcgactccg gcaaaataga tattgatgaa ttgcgcaaac
     8881 aaattggctt tgttacacaa gaaacacaat tatttgccgg cacaataaaa gaaaatatgt
     8941 tgtttgtgca gccggatgca agcgatgctg atatcatcga tgcgcttaac aaagcgtcat
     9001 gtcagagctt actggctcga agtgataaag gaattgattc ggtaatcggt gaaggcggca
     9061 tgaaaatatc cggcggagaa aaacagcgtt tatctgtagc tcgcgctttg ctgagacatc
     9121 cgaacttatt ggtttttgat gaagccactt ctgctttaga ttcattaacc gaagaggaaa
     9181 ttac
//
LOCUS       DAOQII010000084         9179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4013_length_9179_cov_5.810609, whole genome shotgun sequence.
ACCESSION   DAOQII010000084 DAOQII010000000
VERSION     DAOQII010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4013_length_9179_cov_5.810609"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLF48_05530"
     CDS             complement(<1..953)
                     /locus_tag="PLF48_05530"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HNF48821.1"
                     /translation="MKKIYAVLLLLTSVSTVFAVQKNYNVTIEVKGISNDIGILAYYY
                     GDKRLIQDTVKFDAKGIGKIQGKKEIPAGVYLLAFPKLKYQSFDIILKEPEFSITTDT
                     LDLVNHAFVKNSVENKQMFDDMKYMLPLGKQNDSIYKANKDLKKGDLKYNEYVKVSEK
                     INEQMLTHRKEMIKKYPNTFYSKLIKLMLDTDVPEAPKKQDGTIDSTFKYTYTKQHYF
                     DHLDYNDSGMVRSPVLGGKITRYFDNYVYPHPDSITQEIDKFLAKTRNNRELFMFTLN
                     TLFMKYAKSEIMGQDAVFVDMAEKYYLSGVAWWPDPKGLAEL"
     gene            1089..2603
                     /locus_tag="PLF48_05535"
     CDS             1089..2603
                     /locus_tag="PLF48_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF48822.1"
                     /translation="MSYFNADHDLFRQTFRTFLDKEIMPHIEQWEEDGEVPDEVFQRF
                     GDMGFFGLTQEEAYGGSNLDFWYDVIFIEEISKCESGGFGASLSAHPYLTLSHLKHEG
                     SPFLKEKYLKKGISGEWHGALAITEPHAGSDVAGIKTTAVKDGDAYIINGSKCFITNG
                     VSADYYIVACKTRPDAGASGISLIIVDTQSAGITKSPLKKLGWKASDTAEIAFDQVRV
                     PADNLLGEENKGFYYIMQRFELERLTLALGAIASSEWALDYTLKYMNERKAFGRTINK
                     FQVLRHKIAQMYAELTAVKTFCYHICDLYSKGKYCVKEASMVKLLATELSDKIAYQCL
                     QMFGGYGYMEEYKIARFFRDSRLGTIGGGTSEIMLEIISKMVMDEVSYKLKDNSQSPT
                     AESNRFDSVAKIFATLPSRFKTEKAKDIKLHVVFKFDTSNYRVMIQDGNLEIQTAVEN
                     ELKIADCLVETDDATYIAVETGSMNPQEAFMSGKIKVSDLSKMMQFGSLFRKLK"
     gene            complement(2639..3658)
                     /locus_tag="PLF48_05540"
     CDS             complement(2639..3658)
                     /locus_tag="PLF48_05540"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF48823.1"
                     /translation="MVFDDSLIFIVGNSRSGTTLLMRVLEHHQNVFVFNELHFFEELW
                     SPDDKNKKLSVSEAVELYSKLLLTQRIGYKTHDQNYHQFDAESEEVIKLLPQDKLTGE
                     FVFTEMLKREAALHHKTIICEKTPQNVFYLKEILEIYPNARIINMVRDPRAIMLSQKN
                     KWNRRKLGSDYMTEKEALRLRINYHPITISKLWNAAITAARKMETDSRIITVRFEDLL
                     DQPETIIRKVCKHLGIAYHENMLQIKQVSSSNEADSKDIGFKKERASNWKKGGLNKAE
                     ICWNEKICGDNMLHYGYTLDKPDTGVFQIAAYFISFPFKLILALFFNLDRMKNIAETL
                     KRRLK"
     gene            4054..5598
                     /locus_tag="PLF48_05545"
     CDS             4054..5598
                     /locus_tag="PLF48_05545"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNF48824.1"
                     /translation="MKARGFIFLQLLLISVSIVGQTITTTPVTGAVTENSAKLLFFTK
                     KESKGVVHVYLNQNTKYELSFPFQTTNQVAHLQLNHLQPDYIYQYDIQLNGDSSHYRG
                     SFKTFPHSGYPSSFSFTFGSCTEQHHRDSIFIEMQKQHPLFFLHLGDWLYSDNLKNGN
                     FYYLENIERVKEIYQLRYTMPNLSKLMQETAIDYMFDDEDGVHDDFSKSTYSEIQRQD
                     NRITCKEIPYDTSYKQSLYMGMSRFFPSYPEVNTKQAYHSFVCGNAEFFILDTRSTRS
                     PNLEVLQKKGKYWKYRVPENHTILDTAQQYWLKRKLKDSKAQWKFIISGVTFNASYKK
                     LMNLMLLKPVQNKIIYKNMTGAYIAASLSTMWFAFPESQTDLINYCADNQIKNVIVLS
                     GDAHTVAIDDGINSGFPELMAASLAQENSKLAGIIYNQLKLDLWNKGGQGINNTNYND
                     AFGKVTVNGNSSVTLSAIDTYGNLICSYDVKDGYLPKKINYKKARKISFGNKVRAAKQ
                     AFKISR"
     gene            complement(5626..6456)
                     /locus_tag="PLF48_05550"
     CDS             complement(5626..6456)
                     /locus_tag="PLF48_05550"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF48825.1"
                     /translation="MRKYTLTISLFCLCFLLSFAQKNGKNIGGIGGQALFDSISKRPI
                     VKSLEINNYFTAHTLKPGDLILEVDKVPVHNMSYSEVLGLVQGVIGTPMSLKILRYNG
                     IEKYYEIKRIRVTLDMNPTWWSVPDYRYFELMDGINYTISQLRVNGRNSIDTTKYRMA
                     NDRYFCNFNIRGAYESVYINKGGRLSYSCSFLKSDDRSKAEGMYVWLKSQMTNLKIPF
                     VKLQKNEKVLTDSKDFIFNPKETSDLTLANLKINARMFKEFDKEENKDLWKVELDISM
                     "
     gene            6626..7291
                     /gene="def"
                     /locus_tag="PLF48_05555"
     CDS             6626..7291
                     /gene="def"
                     /locus_tag="PLF48_05555"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNF48826.1"
                     /translation="MFYSGIRGSLELVYTDFYFSKKSMEKCIFAKITMILPITIYGDP
                     VLKKKGVEIGTDYPNLKPLIANMFQTMYGANGVGLAAPQVGLSIRLFIVDTQQLEERK
                     KKDSNFSGIKKAFINPVILEQSGDEWKYEEGCLSIPGIREDVARQAKIKIRYYDEDFN
                     EHIEEHDDINARVIQHEYDHIEGILFTDRLGALKRKMILPKLNKLLKGITDTDYKVKI
                     YKK"
     gene            complement(7294..7917)
                     /locus_tag="PLF48_05560"
     CDS             complement(7294..7917)
                     /locus_tag="PLF48_05560"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF48827.1"
                     /translation="MRRITTLLLFLFSLMAQAKEVVQFSGFIKDATSNAPLPFCTIYI
                     QGENRGTISNLDGYFNFVAAKGDTIIVSSVGYKKFSIIIPADLDKNSFYKEVGIERDV
                     IELPGPVIYPLPTPGQLGQAMINLDIPDNMQELAQQTIEKSILTDELSRKMNYSGNEN
                     YNQYVQQQVGYYYNRYGGQRPGISLTNPFAWAQFIKSIKDKKKKKKK"
     assembly_gap    8004..8103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8154..>9179)
                     /locus_tag="PLF48_05565"
     CDS             complement(8154..>9179)
                     /locus_tag="PLF48_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF48828.1"
                     /translation="DYPWWKYFERPSVKYSDYKYVAEKSKLADYVTFEINPRQFPEVS
                     YKDKKTRKANISGVMDNYGKIFNLETEKGRFLSSMEIANGINVCVIGKDIAEKLFNPN
                     EEILDKQIKIDGKEVKIIGVIKKKGQDLLGFNYDKSIVVPYNFYVRNFSDDDNQTDKT
                     VEVHIRPGESMDELKNELIALMRSSKKLRPKQDDNFSLNELSVIAKGFDGLFSGLDIV
                     GLIIGGLAALVGGFGIANIMFVSVSERKAVIGIKKALGARRRDILTEFLLESVFLCLI
                     GGAIGLLLVYLLAIAATNGTGMEFTLNLKNSLIAIFLSIILGILSGIIPAFLAAKLDP
                     VEAIRSK"
BASE COUNT         2874 a         1684 c         1619 g         2902 t
ORIGIN      
        1 agttcggcta atcctttcgg gtcaggccac caggctactc cgcttaaata atatttttct
       61 gccatatcaa caaaaacagc gtcttgcccc attatttcac ttttggcata tttcataaac
      121 aaggtgttta atgtaaacat aaacagctct ctgttgtttc ttgttttagc caaaaactta
      181 tctatttcct gtgtgatgga gtcaggatgc gggtagacat aattatcaaa gtaacgggta
      241 atttttcctc ccaataccgg cgaacgcacc atgccggaat cgttataatc cagatggtca
      301 aaatagtgct gtttggtata ggtgtattta aatgtagaat caatagtgcc atcttgtttc
      361 ttaggtgctt ccggcacatc cgtatccagc atcagtttta ttaacttgga gtaaaaggta
      421 ttcgggtatt tttttatcat ttctttgcgg tgtgtcagca tctgttcatt tattttttca
      481 gaaaccttga catactcatt atatttcaga tcgccttttt tcaagtcctt atttgctttg
      541 tatatggaat cattttgttt gcccagtggc aacatatatt tcatgtcgtc aaacatttgc
      601 ttgttttcca ccgagttttt tacaaaggca tgattaacta aatccaacgt atctgtagtg
      661 atactgaatt caggttcttt taaaataata tcgaatgact gatattttaa tttgggaaat
      721 gccaacaaat aaacgccggc cggaatttct tttttcccct gaattttacc gattcctttt
      781 gcatcaaatt ttaccgtgtc ttgtatcagg cgcttgtctc cgtaatagta agctaaaatt
      841 ccgatatcgt tggatatgcc ttttacttct atggttacgt tataattttt ctgcacggca
      901 aaaaccgtag aaacacttgt taataaaaga agtacagcgt agattttttt cataaatcgc
      961 aattacgtag gaggttaatc aaattttgtt ccgaaattat tgattcctcc cgaaatattg
     1021 aactaaatct ttgttaattc aatacagaat aagctatatt cgggcaataa gaaaatattt
     1081 tttgaaagat gagttatttt aacgccgacc atgatctgtt tcgccagacc ttccggactt
     1141 ttttggataa agaaatcatg cctcatattg aacaatggga agaggacggt gaagtaccgg
     1201 acgaagtatt tcaaaggttt ggtgatatgg ggtttttcgg cttgacacaa gaagaagcat
     1261 atggaggctc taatctggat ttctggtatg atgtcatttt tatagaagaa atttctaagt
     1321 gtgaaagtgg aggatttggg gcgagtttat ctgcacatcc ttacctcaca ttatctcatc
     1381 taaaacatga aggttcgcct ttcttaaaag aaaaatattt gaaaaaaggc atcagcggtg
     1441 agtggcatgg tgcattagcg attacggaac cacatgcagg cagtgatgtg gccggaataa
     1501 aaacaacggc agttaaagat ggtgatgctt atatcattaa cggctcaaaa tgttttatta
     1561 ccaatggcgt atccgcagat tattatattg tagcttgtaa aactcgacct gatgccggag
     1621 caagtggcat ttcattaatc attgtcgata cccaaagtgc aggtataaca aaatctccct
     1681 taaaaaaact gggctggaaa gccagcgata cggcagaaat agcttttgat caagttcgag
     1741 ttccggcaga taatttattg ggcgaagaaa acaaaggttt ttattacatc atgcagcgtt
     1801 tcgagctgga gcgtcttaca ctggcattag gtgcaatagc ctcctcagag tgggcgctgg
     1861 actacaccct taaatacatg aacgaacgaa aagcatttgg cagaactata aacaaatttc
     1921 aggtattgcg gcataaaata gctcaaatgt atgctgagtt gactgctgta aaaacttttt
     1981 gttaccacat ttgcgattta tacagcaagg gtaaatattg cgtgaaagaa gctagcatgg
     2041 taaaattgct ggctacagaa ttatccgata aaattgccta tcagtgcttg caaatgtttg
     2101 gcggctatgg ctatatggaa gaatacaaaa ttgcccgttt tttcagagat tccagattag
     2161 gaacaattgg tggcggaaca tcagaaatca tgctggagat aatttctaaa atggtgatgg
     2221 atgaagtgag ttataaattg aaagacaaca gtcagtcacc gactgctgaa tcaaatagat
     2281 ttgattcagt agccaaaatt tttgcgacat tgccttcaag attcaaaact gaaaaagcaa
     2341 aagatattaa attgcatgtt gtgtttaaat ttgatacttc aaactacaga gtcatgattc
     2401 aagacggaaa tttggaaatc caaacagctg tagaaaatga acttaaaatt gcagattgtt
     2461 tagtagaaac cgatgatgct acttatattg ccgttgaaac aggaagcatg aatcctcagg
     2521 aagcgttcat gtctggcaaa attaaggtaa gcgatttgag caagatgatg caatttggca
     2581 gtttgttccg caagttgaaa taaatcaaat cagcacaatt gtattactct ttttaaagct
     2641 acttcaaacg tctttttaaa gtttccgcta tgtttttcat tctgtctaaa ttgaagaata
     2701 aggccaatat cagcttaaac ggaaatgaaa taaaataagc cgcaatttga aaaacaccgg
     2761 tatccggctt atctaacgta taaccataat gcaacatatt atctccgcaa attttctcat
     2821 tccaacagat ttctgctttg tttaatcctc cctttttcca gttgctcgcc ctttcttttt
     2881 taaatccgat gtctttactg tcggcttcat ttgaagaact gacttgtttg atttgcaaca
     2941 tattttcgtg gtatgcaatt ccgaggtgct tgcacacctt gcgtatgatg gtttccggct
     3001 gatcgagcaa gtcctcaaac cgtaccgtta ttattctgga gtcagtttcc attttacggg
     3061 ctgcagtaat ggctgcattc cacagttttg aaatggtaat gggatgataa ttaattcgca
     3121 aacgcaaggc ttccttttct gtcatataat cacttcctaa tttgcggcga ttccatttgt
     3181 ttttttgcga gagcataatt gcacgcgggt cgcgcaccat atttataatt cgggcattcg
     3241 gataaatctc cagaatttct ttgagataga aaacattttg cggggtcttt tcacaaataa
     3301 tagttttgtg gtgaagagcc gcttcacgct tcagcatttc cgtaaaaaca aattctccgg
     3361 tcagtttatc ttggggtaaa agtttaatga cttcttcgct ttctgcatca aactggtgat
     3421 agttttggtc gtgtgtttta taaccaattc tttgtgtaag cagtaatttg gaatagagtt
     3481 caacagcctc tgatacagat agttttttgt ttttatcatc cggcgaccat agttcttcaa
     3541 aaaaatgaag ttcattaaac acaaaaacat tctggtgatg ctcgagtacg cgcattagca
     3601 aagttgtacc actgcgcgaa tttcccacta taaaaatcaa actatcatca aacaccattt
     3661 tgcaaaggta cagaatgtgt atatatttta tatggaaagt atctttgtga tacaaccgct
     3721 tcattgattt gaaaatatta gttttaacat aatgtaatat ttgttttata atctttacca
     3781 catagaagca taaattcttt ttaggattaa aaatgattac catacaaaga ttgaggtaag
     3841 aatagaaaaa cataggaatc tccattggcg atactatgtg tttgcttgaa taactatact
     3901 aatttgctgc aaacaactaa ttatcattaa aaatgctatg catctatgtg gttaaactgc
     3961 acaataaatt aatttcatta agcatattga aattactgta agacttaaca ttacattact
     4021 gaaagacttt gcattttttt atacttttaa aagatgaaag ctcgaggctt tattttttta
     4081 caacttttac tcatttctgt ttcaatagtt gggcaaacaa ttaccaccac acctgttaca
     4141 ggagctgtta ctgaaaattc tgccaagcta ttatttttca caaaaaagga atcaaaaggc
     4201 gtcgtacatg tgtatctcaa tcaaaacact aaatacgaac ttagtttccc gtttcagacc
     4261 acaaatcaag tagcacattt acaattaaac catctccagc ctgactatat ttatcagtat
     4321 gacatccaac tcaatggcga cagcagccat tatagaggct ctttcaaaac attcccgcat
     4381 tccggatatc cttcttcatt ttcattcacg ttcgggtctt gcacggagca gcatcataga
     4441 gattctatct ttatcgaaat gcaaaaacag catcctttgt tttttttgca tctcggcgac
     4501 tggctgtatt cggataatct taaaaacggg aatttttatt acctggaaaa tattgaacga
     4561 gtaaaagaaa tttaccaact gcgatacaca atgcccaact tatctaaact gatgcaagaa
     4621 acagccatcg actacatgtt tgatgatgag gatggcgtgc atgatgattt ctcaaaatct
     4681 acttattccg aaatacaaag acaagacaac cgtattactt gcaaagaaat tccatatgat
     4741 acaagttata agcaaagtct gtatatggga atgtcccgtt ttttcccgtc ttaccctgaa
     4801 gtgaatacaa aacaggctta tcatagtttt gtttgtggca atgctgaatt ttttatctta
     4861 gatactcgct ctactcgttc gcccaattta gaagtactgc aaaaaaaggg gaagtattgg
     4921 aaatacagag tacccgaaaa tcatacaatt ctggatacgg cacagcaata ctggcttaaa
     4981 agaaagctga aagactctaa ggcacaatgg aagtttatta taagcggggt tacttttaat
     5041 gcatcgtata aaaagctgat gaatttgatg ttgcttaagc cggttcaaaa taaaattatt
     5101 tataaaaaca tgaccggtgc gtacattgca gcatccttat ccaccatgtg gtttgcattt
     5161 cccgagtcgc aaactgattt aattaattat tgcgccgata atcaaataaa aaatgtaatt
     5221 gtactcagcg gagatgcaca tactgttgca attgatgatg gtataaattc aggattcccg
     5281 gaactaatgg cggcatcgct ggcacaagaa aactcaaaat tagccggcat tatttataac
     5341 caactcaagt tagatttatg gaataaaggc gggcaaggta tcaacaatac gaattacaac
     5401 gatgctttcg gaaaagtaac cgtaaacggc aacagctccg ttaccctctc ggcaatcgat
     5461 acgtacggaa atttgatttg cagctatgac gtgaaagacg gttatctgcc aaaaaaaata
     5521 aactataaaa aagccagaaa aatatcattt ggaaataaag taagagcagc aaaacaagct
     5581 tttaaaatca gccgatgagc attgatttta ctgaaaacag cttttttaca tgctaatatc
     5641 caactctact ttccacaaat ctttattttc ttccttgtca aactctttga acattctggc
     5701 attaattttc agattagcta aagtcaaatc agaagtttct ttcggattaa atataaaatc
     5761 ctttgaatcg gttagtactt tttcattttt ctgcaacttt acaaaaggaa ttttcagatt
     5821 agtcatttgc gatttcagcc agacatacat accttctgct ttagagcggt catcagactt
     5881 taagaatgaa caactatagc ttagcctgcc tcctttgttg atataaaccg actcataagc
     5941 tcctctaatg ttaaagttac agaaatatct gtcgtttgcc attctgtatt tagtggtgtc
     6001 tatagaattt cttccgttta ctctgagttg agaaatcgta taatttatac catccatcag
     6061 ttcaaaatag cgataatcag gcaccgacca ccaggtagga ttcatatcta atgtaacacg
     6121 gatacgttta atctcataat atttttcgat accgttatag cgcagtattt tcaagctcat
     6181 cggtgtgcca attactcctt gtaccaatcc caaaacctcg ctataactca tattgtgaac
     6241 cggcacctta tctacttcca aaatcaaatc tccgggtttt agcgtatggg cagtaaaata
     6301 gttattgatt tcgagagatt tcacgatagg ccgcttagag atactgtcaa ataaagcctg
     6361 ccccccaatg cctccgatat tcttaccatt cttttgagca aaagaaagca agaagcaaag
     6421 gcaaaacaaa cttattgtta atgtatattt tctcactaca aaataaatta caaatattat
     6481 tccacaaaaa aatgccctcc ggagaaggca tttatgtaac gcaaaaatag tatttatatt
     6541 tttcatgtaa aagtgcaaat acatttctta atgataatta aatcaaagtg aggctaatca
     6601 gaactatata aaataaaaca ttaatatgtt ttattccggc atacggggtt ctttagagtt
     6661 agtttatacg gatttttact tttcaaaaaa atcgatggaa aaatgtattt ttgcaaaaat
     6721 tacgatgata cttccaataa cgatatacgg cgatcctgtt ttaaagaaaa aaggtgtgga
     6781 aattggtacc gattatccta atttgaagcc actcattgcc aacatgttcc aaactatgta
     6841 tggtgcaaat ggggtaggtt tggcagcgcc tcaagttggt ttatccatac gcttgtttat
     6901 cgtagacacc cagcaactgg aagaacgcaa gaaaaaggat tccaatttca gcggtattaa
     6961 aaaggctttt attaatcctg taattctgga acaaagtggt gatgagtgga aatacgaaga
     7021 aggttgtttg agtattcccg gtattcgaga agacgtagcg agacaggcga aaataaaaat
     7081 ccgctattat gatgaggatt ttaatgagca tattgaagaa cacgatgata ttaacgccag
     7141 agtgattcag catgaatatg accatataga aggcatatta tttaccgaca ggttgggcgc
     7201 attaaaaaga aagatgattt tgcctaaact caacaaactg ctgaaaggaa taacagatac
     7261 agattacaaa gtaaaaatat acaagaagta attttatttc ttctttttct tctttttgtc
     7321 cttaatactt ttgataaatt gtgcccaggc aaaaggattt gtcagtgaaa ttcccgggcg
     7381 ttgaccgccg tagcgattgt agtaataacc tacttgctgc tgaacatatt gattgtaatt
     7441 ttcattaccg ctgtaattca tttttcttga caactcgtca gttagtatag atttctcaat
     7501 agtttgttgt gccagttcct gcatattatc cggaatatcc agatttatca tagcctgacc
     7561 taactgaccc ggcgtgggaa gcggataaat tacaggtccg ggcagctcta ttacatcgcg
     7621 ttctatgccc acttctttat aaaaactgtt tttatccaaa tctgcaggaa taatgatgct
     7681 gaatttttta tatcctactg atgatactat aatcgtgtcg ccttttgcag ccacgaaatt
     7741 aaaatagccg tctaaattgg atatagtgcc tctgttttcg ccttgaatat atatagtaca
     7801 aaatggtagc ggagcattcg aggtagcatc tttaataaaa ccggaaaatt gcaccacttc
     7861 tttggcctga gccatcaaac tgaacaaaaa aagaagtagt gtggtaattc ttcgcataat
     7921 ttgatactat tcctgcaaaa gtaataccaa ttatcggtta aatttagtgt ttaatattaa
     7981 ataacagatg agaaactagg atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnagtagta agggttagta taaggaacga tgatttctca aattatggga ttgctattta
     8161 cttctgattg cttccacggg atcgagtttg gcggccaaga atgccggaat aatcccggat
     8221 aaaatcccta aaataataga cagaaagatg gcgattagtg aatttttcag attaagcgta
     8281 aattccatac cagtgccgtt tgtagctgca atggctaaca aatagaccaa tagcaaacca
     8341 attgcgccac caatcaaaca taaaaaaacg gattccagta aaaactcagt aagaatatct
     8401 ctgcgtcttg ctccgagtgc ctttttgatg ccgataacag cctttcgttc agatacggaa
     8461 acaaacatga tattcgctat accaaatcca cccaccaaag ccgcaagtcc gcctataatc
     8521 aaccctacaa tatccagtcc gctaaatagt ccgtcaaatc cttttgcaat tacacttagt
     8581 tcattgagtg aaaaattatc atcttgttta gggcgcagtt ttttcgatga acgcattaga
     8641 gcaattaatt cgttttttag ctcatccatg ctttcgcctg ggcggatatg cacttctacc
     8701 gttttatccg tctgattgtc atcatcacta aaatttctga cataaaagtt atacggcact
     8761 acaattgatt tatcataatt aaacccaagc agatcttgcc cttttttctt gatgactcca
     8821 attattttga cttcttttcc atcgattttt atttgcttgt ccagtatttc ttcatttgga
     8881 ttaaacagtt tttctgcaat atctttccca atcacacaca catttatacc gttagctatt
     8941 tccatggaag atagaaacct tcctttctct gtttccagat taaagatttt tccatagtta
     9001 tccatcactc ctgaaatatt ggcttttcta gtttttttgt ccttgtagct cacttccgga
     9061 aattgccttg ggttgatttc aaaagttaca taatctgcca gtttcgattt ttctgctaca
     9121 tatttataat cactgtattt cacgctgggg cgctcaaaat acttccacca gggataatc
//
LOCUS       DAOQII010000085         9179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4014_length_9179_cov_4.710105, whole genome shotgun sequence.
ACCESSION   DAOQII010000085 DAOQII010000000
VERSION     DAOQII010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4014_length_9179_cov_4.710105"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLF48_05570"
     CDS             <1..416
                     /locus_tag="PLF48_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48829.1"
                     /translation="PWSEIQSYSINDEANGSIIKLTDKNNHTHTFTFSSVLEDAENYN
                     LFSSTLSRQIALYNEHRADQTSGHAQPTIMKGKNFYGSKGGKFYGIFILLFLVGFPIL
                     VWQFKLPITTISILKIVWIYPLGIYMMYRIFVKGE"
     gene            670..751
                     /locus_tag="PLF48_05575"
     tRNA            670..751
                     /locus_tag="PLF48_05575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Leu,seq:gag)
     gene            complement(758..2158)
                     /locus_tag="PLF48_05580"
     CDS             complement(758..2158)
                     /locus_tag="PLF48_05580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF48830.1"
                     /translation="MHIQFCGAAQTVTGSCHLLTLDNGMKVLLDCGLYQGNEDTMASF
                     NDQWYFKPEEIDLMILSHAHIDHCGRIPKLVKDGFKGKIFCTSATRDLCAIMLLDSAH
                     IQQSDAEYETKKTGKLIRPLYTEEDAKFCLKYFVCVEYAEWFKINKNIELLFSDAGHI
                     LGSATVTLKITEGDKETFVGFSGDLGRYNRPILKDPVPMMELDYLLCESTYGGVKHEE
                     SPESDDRLLQIIKETCIENKGKLIIPAFSIGRTQELLYRMDLLYTSGKLGDIKVYVDS
                     PLAMNATEIFIMHPECYDDDIQHFIEKDENPFGWRNLYFTRNIEQSKRLNNSDEPCII
                     IAASGMANAGRVKHHIFHQIEKPENTILIVGYCAEGTLGEQLVKKPEVVEIFHEVKKV
                     NARIEVMSSMSAHADQPEMLQFLSNQNKQLLKKIFLVHGEPKRQMAFKSALEEAGFKN
                     VSIPYLGETAKLEEVS"
     gene            2242..2712
                     /locus_tag="PLF48_05585"
     CDS             2242..2712
                     /locus_tag="PLF48_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48831.1"
                     /translation="MLESLFGDIQAKIQQQIQERFGLNTDQTSQSTNVLFENFQKFIS
                     QDIFSGGIDGMKDMMNNGINDITNNPKLNEMKENLLNDLVNKVGLSEEMAQKVRDFSI
                     QQVFAEFRNQFLDDRGMPDFSKILSKVNMADFQAQAQELLSKMGGNFGNMFGKQ"
     gene            2824..3105
                     /locus_tag="PLF48_05590"
     CDS             2824..3105
                     /locus_tag="PLF48_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNF48832.1"
                     /translation="MSYNVKIILDGKESQITVHDGETILEAAIAADLDPPYACRVAAC
                     CSCKAKLTAGKVEMDDDEPLTEEELEEGFILTCQSHPKTNDCIVNYDEA"
     gene            complement(3142..3975)
                     /locus_tag="PLF48_05595"
     CDS             complement(3142..3975)
                     /locus_tag="PLF48_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF48833.1"
                     /translation="MSNYNYSKGFKIKKLLFKTFISLIGLYFIACGLLYILQERLIFF
                     PEKLAKNYNFDFKQSFQEINIKTKDSNLLNGLLFTTDCPNGLIFYLHGNAGNLSSWGD
                     VAKTYIGLNYDVFIMDYRGYGKSESCINGQSQMFDDIQIAYNELKKTYHENEIIVLGY
                     SIGTGLAAKLASENNPKLLILQAPYYSLTDMMKHTYPFVPVFLLKYKFPTNDCLKKCN
                     MPVIVFHGNQDEVIYYGSSLKLKKEFKPNDTLITLTGQGHNGITENADYISAMKNLIG
                     N"
     gene            complement(4025..4627)
                     /locus_tag="PLF48_05600"
     CDS             complement(4025..4627)
                     /locus_tag="PLF48_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48834.1"
                     /translation="MIERFLKAKHWKLFLLTFGIPIIFQFILMGFMLSNFTTTSNPDP
                     VEMLSCMKFFPLIMVFFTAIFFGWFWSVAIGLQNKIPKNLTMNVKKFKLFFFIPIIYI
                     FCITIFISYTMDGIIISGNEPNVGLFGSLFAFIVPMHLFSMFCILYTLYFVAKTIKTV
                     ELQKEVKFSDFAGDFILIWFYPIGIWIIQPKINKFIEESK"
     gene            complement(4831..5520)
                     /locus_tag="PLF48_05605"
     CDS             complement(4831..5520)
                     /locus_tag="PLF48_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48835.1"
                     /translation="MNSANFKLAEQEIKKICTNSPKNNCEHVFCKLHEPEYLGSHEKN
                     SHVCVACNLNDSIEKIYKFLKGLKNEDDIEYSFTIFILLCYLSVEKLTIIFKHIGISQ
                     DYVEKNWTILIEIRKWANFIKHPKGFLFSHHPVYLYENEKIDKTYQNWKVINYDNFIE
                     PLYKREDEGKYIQTISQIGNKKDILVIIPNPFRLVTEFNVVCNKICEKIKNNEHFKEI
                     LKHETVIDDYN"
     gene            complement(5647..6216)
                     /locus_tag="PLF48_05610"
     CDS             complement(5647..6216)
                     /locus_tag="PLF48_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNF48836.1"
                     /translation="MKNEIRIAITDKQRTALISTLKMRFEKYSIRHKNIEWESLEIKL
                     KAQPDKLNILFLMEDTGGEPDVVAYDKNTGQYTFFDCSEESPKGRRSLCYDHKALEAR
                     KEHKPQNSAQQMAAEIGIALLTEAQYRALQQLGQFDTKTSSWIATPENIRVLGGAIFA
                     DRRYGQVFIYHNGAESYYAARGFRGCLIV"
     gene            complement(6322..6765)
                     /locus_tag="PLF48_05615"
     CDS             complement(6322..6765)
                     /locus_tag="PLF48_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF48837.1"
                     /translation="MAQINPYIHFNGNAEEAFNFYKSIFGGEFAMLVRYKDMANPHNP
                     NAEKEADKIMHIALPIGKHNVLMGSDTPEFMGRHNENENRSKISISAESKEEADHLFN
                     GLSADGQVEMPIADSPWGSYFGMFRDKFGFEWIVDFDPKYNGQTT"
     gene            complement(6796..7245)
                     /locus_tag="PLF48_05620"
     CDS             complement(6796..7245)
                     /locus_tag="PLF48_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF48838.1"
                     /translation="MANNTVKLHRVFSAPVEKVFRAFSDPDAMASWIPPYGFICKVHS
                     MDFKVGGTYKMSFTNFTTGSSHNFGGEYVEIIPDEKIKYTDKFDDPNLPGQMITTIEL
                     KKVLCGTEIIATQEEIPDAIPVEMCYLGWQESLDKLKRLVEPNIPDA"
     gene            complement(7466..>9179)
                     /locus_tag="PLF48_05625"
     CDS             complement(7466..>9179)
                     /locus_tag="PLF48_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48839.1"
                     /translation="KMSEIDYAYELEKNRKVELKCKGTDLCVYSTFTGANYNTFTFSQ
                     ATDFNTGEFISLLNNLVKAYHKNLTAKNKEAEEYGNLQDDLDKYKSNKDNMSSGTTSS
                     SKQTTSGNYQAELKKLNDYLKTFDNGYYGYWEVKDGYIYDRFKAGKYNKFKMEDMEGA
                     IIQAEYSRVIFKCKTGDCISTDWKPNGKEPYTQFTKSGSYNYKELADLLNNFRDAYLS
                     KKKQNTSSNNSEKYTESSEFLNGYESNKDNMNSGTNKTTSSSKTTSSSKAENIEKANK
                     ALQELNNLMVTLDNGRYVGMEVTDGYIINRYQNNQYSKAKIEDIDHVEINTQYNYAKL
                     ACKGESKCIYSTITGSYHDYFNFNASGSKLTQMEKLLNNFLTALKKCTDPNLSSSKTT
                     STSTSRRDEEAKKRLENANKSGSNKKTEDEDDFYWIDEDFDVLANTQTDKKETTKSSG
                     TTSSTSASNKYADALKKLNAYLQIYNKETYRDIEVKDNDVYFKYYSSGLNYASTISIS
                     KLKQNTYIEDYSSNTIRLKCKTGSCFWSEYSDDYADHFQFFTYSNGDKNKLKKLLQDF
                     IAAL"
BASE COUNT         2795 a         1579 c         1482 g         3323 t
ORIGIN      
        1 tcccatggtc agaaatacaa tcttattcaa tcaatgacga agctaatggc agtattataa
       61 aattgacaga caaaaataat catacccata cgtttacttt cagcagtgtg ctggaagatg
      121 cagaaaatta caaccttttt tcttcaacct taagccggca aattgcttta tacaatgaac
      181 atagagccga tcaaacatca ggacatgcac aaccgacaat catgaaaggg aaaaattttt
      241 atggcagcaa aggcggaaaa ttttacggca tttttatctt gttatttcta gtgggatttc
      301 ccatattagt ttggcaattt aaacttccca ttacaacaat aagcatattg aaaattgttt
      361 ggatttatcc actcggaatt tatatgatgt atcgcatttt cgtaaaagga gagtaggttg
      421 taataagaag aatacgaaaa agttaaaaac ctaagtttgt gctaagaaaa ataaggtaaa
      481 aaaaaatagt tgatttctca ttttccctcc aacttaacaa gacggaagaa taaaaaggcg
      541 gtggtgcttg attataaaat caactgtttt taaattgaaa cgcttatcct gaattcaggt
      601 tataagtaaa tttgcacatt ttttttgtac tctttatccg atactgtatc tttgcaactt
      661 aatttttctg cccacgtgga gaaattggta gactcgccat cttgaggggg tggtgctgca
      721 aggcatgctg gttcgagtcc agtcgtgggc acttcattta gcttacttct tctagcttag
      781 ctgtttctcc caaatatggg atggaaacat ttttaaatcc ggcttcttcc aaagcagact
      841 taaatgccat ttgtcttttc ggttcaccgt gtaccaaaaa tatttttttc agtagttgtt
      901 tattttgatt ggataaaaat tgaagcattt caggttggtc ggcatgtgca ctcatactgc
      961 tcatcacttc tatacgcgca ttcacttttt ttacttcatg aaaaatttca accacctccg
     1021 gttttttcac cagttgctca cctaaagtac cttcggcaca atagccaact atcaaaatgg
     1081 tgttttcggg tttttctatt tggtgaaaga tatggtgctt cactcgtccg gcattggcca
     1141 tacccgaagc agcaatgatg atgcatggct catctgaatt gtttaaacgc ttggattgct
     1201 cgatatttct tgtaaaataa agattccgcc acccgaaagg attttcatct ttctcaataa
     1261 aatgctgaat atcatcatca taacattcgg gatgcatgat gaaaatctct gtggcattca
     1321 ttgccaaggg actatccaca tacactttta tgtcgcctag ttttccggat gtataaagta
     1381 aatccatgcg gtacagcaat tcctgcgttc tgcctatgct aaatgccgga ataatgagtt
     1441 tacctttgtt ttcaatgcaa gtttctttta tgatttgcaa gagtctgtca tcactttcgg
     1501 gtgattcctc atgttttaca cctccataag ttgattcgca caataaataa tccagctcca
     1561 tcatcggcac agggtctttt agaataggtc gattatacct gcccaaatcg ccactgaagc
     1621 ctacaaaagt ttctttgtcg ccttcagtta ttttcaaagt aacggtggca cttcctaaaa
     1681 tatgtccggc atcagaaaac aacagttcta tatttttatt tatcttaaac cactctgcat
     1741 attcaacaca aacaaaatac ttcagacaaa acttagcatc ttcttcggta tagagtggcc
     1801 tgattaactt cccggttttt tttgtttcat attctgcatc actttgttgt atgtgggctg
     1861 aatcgagcaa cataatagca cataaatccc gagtagctga tgtacagaat atttttcctt
     1921 taaaaccatc ttttactaat tttggaattc tgccacagtg atcaatatgt gcatgcgaca
     1981 aaatcatcaa atcaatttct tccggcttaa aataccattg atcattaaat gaagccattg
     2041 tatcttcgtt gccttgatat aaaccacaat caagtaatac tttcatccca ttgtctaaag
     2101 taagcaggtg acaactaccc gtaacagttt gtgcggctcc acaaaattga atatgcatac
     2161 taatttgttt tgtcactaaa agtaaaaaac attttgattt aattttgaat tttggcttat
     2221 atttgttcta aactacacac catgttagaa agtttattcg gcgatattca agcaaaaatt
     2281 caacaacaaa tacaagaacg atttgggttg aatacagatc aaacatctca aagtactaat
     2341 gtgctttttg aaaacttcca gaagtttatt tctcaggata ttttttcagg aggaatagac
     2401 ggaatgaaag atatgatgaa taatggcatc aacgatatta ccaataaccc gaagctcaat
     2461 gaaatgaaag aaaatttgtt gaatgatttg gtgaataaag taggattgag tgaggaaatg
     2521 gcacaaaaag tcagagattt ttctattcag caagtatttg ccgaattcag aaatcaattt
     2581 ttggatgatc gcggtatgcc tgatttttca aaaatactca gcaaagtgaa tatggctgat
     2641 tttcaggctc aagctcaaga actgctgagt aagatgggtg gaaacttcgg gaatatgttc
     2701 ggcaaacaat aagcaattta ttttgcattt ctttactaag gatagaaagt tgaactttta
     2761 cttagaaatg tcatattaat tctgatttct tttttagatt cacatatcac taaataacag
     2821 ggcatgtcat ataatgtaaa gatcatctta gatggcaagg aaagtcagat tacggtacac
     2881 gatggcgaaa ccatattaga agcggccata gcggctgatt tagacccgcc atatgcctgc
     2941 agggtggctg catgttgcag ttgcaaggcc aaactgactg ccggaaaagt ggaaatggat
     3001 gatgatgaac ccttgaccga agaagaactg gaagaaggtt ttattttaac ttgccaatca
     3061 caccccaaaa ccaacgattg tattgtcaac tatgatgaag cttaaggaat gttggatgtt
     3121 aaatgttgaa tttaaatgtt gctaattgcc aatcagattt ttcattgcag aaatatagtc
     3181 agcgttttct gttattccgt tatgaccttg tccggttaat gtaattaatg tatcattcgg
     3241 tttaaattct ttttttagtt tcagagatga accataatag ataacctcat cttgatttcc
     3301 gtgaaaaaca attaccggca tgttacattt ttttaagcag tcattggtag gaaatttata
     3361 tttcaataaa aataccggaa caaaaggata ggtatgtttc atcatatctg tcaggctgta
     3421 ataaggggct tgtaaaatca aaagtttagg attgttttct gaagcaagct tagcagcaag
     3481 tccagttcct atggaatacc ctagtacaat aatttcattt tcatgatatg ttttttttag
     3541 ttcgttataa gctatttgaa tgtcatcaaa catttgactt tgtccgttta tgcagctttc
     3601 actttttcca tatcctcggt agtccattat aaaaacgtca taatttaaac ctatgtatgt
     3661 ttttgcaaca tcaccccagg aacttaagtt gccggcattt ccatgtaaat aaaatatcaa
     3721 tccattaggg caatctgtag taaataaaag accatttaat agattactat ctttcgtctt
     3781 tatatttatt tcctgaaagg attgtttaaa atcaaagttg taatttttag ctaatttttc
     3841 agggaaaaaa ataagtcttt cttggagaat atacagcagc ccgcatgcaa tgaaataaag
     3901 tccaattaaa ctaatgaatg ttttaaatag tagttttttt attttaaatc ctttagagta
     3961 gttataattt gacaaaataa ataattattt taaatgtata tagatattgt ttcacttttt
     4021 cagcctattt tgattcttct atgaatttat ttattttagg ttgaattatc catattccaa
     4081 tgggataaaa ccaaatcaaa ataaaatcac ctgcaaaatc tgaaaatttc acttcttttt
     4141 gtaattctac agttttaata gtttttgcaa caaagtacaa cgtataaagg atacaaaaca
     4201 ttgaaaataa atgcataggc acgatgaaag caaacagact tccaaaaagc ccaacatttg
     4261 gttcgtttcc gcttatgatt attccatcca ttgtatatga tatgaaaatt gtaatacaga
     4321 aaatatatat aatcggtata aagaaaaata gtttaaactt tttcacattc atagttaaat
     4381 ttttaggtat tttattttgt aggcctattg caactgacca aaaccaacca aaaaatattg
     4441 ctgtaaaaaa taccataatt aagggaaaaa atttcataca gctaagcatt tccactgggt
     4501 caggatttga ggttgttgta aaattggaaa gcataaaacc catcaaaata aattgaaaaa
     4561 ttattggaat tccaaatgtc aaaagaaata gtttccaatg ttttgctttt aaaaatcttt
     4621 ctatcatttt aaatttaatt tagtttttaa tatcttgcat gtgtttttag tataaagtta
     4681 tgtatttctt cttttacaac taacgtttaa aaattgcgat gtttatatat gtttagcacc
     4741 aatcaacact tatttacaaa ttaaatataa taaacaaaac ttgaattacc aaatctacca
     4801 caacgattgc aatttttttg ttagcagcaa ttaattgtag tcatcaatta cagtttcatg
     4861 ttttagtatt tctttaaaat gttcattgtt ttttattttt tcacaaattt tgttacatac
     4921 aacattgaat tcagttacaa gtctaaatgg atttggaatt attactagaa tatccttttt
     4981 atttccaatt tgagaaattg tttgtatgta ttttccttca tcttctcttt tatatagtgg
     5041 ttcaataaag ttgtcataat taatcacttt ccaattttga tatgttttgt ctattttctc
     5101 attttcatat agatatactg gatgatgaga aaataaaaaa ccttttgggt gcttaataaa
     5161 atttgcccat tttctaattt caattaaaat tgtccaattt ttttctacat agtcttgcga
     5221 aataccaatg tgtttaaata ttattgtaag tttttcaaca gataggtaac acaatagaat
     5281 aaaaatagta aatgaatatt ctatatcatc ctcatttttt aatcctttta agaatttgta
     5341 tattttttca attgagtcat ttaaattgca agcaacacaa acatggctat ttttttcatg
     5401 agaacctaaa tattcaggtt catgcaattt acaaaaaaca tgttcgcaat tattcttagg
     5461 tgaattagta catattttct taatttcttg ttctgctaat ttgaaatttg cggaattcat
     5521 atttaattgc actttactcc caaatatacg aaacatttat tcgttaatac tgctttcaat
     5581 tttacgttat tcgtttttgt aaagagcaat gacgattttg gtattgtcta attctatttc
     5641 tcaagtctat acaatcaaac atccgcgaaa tcctcttgct gcatagtaag attcggctcc
     5701 gttgtgatag atgaaaactt gcccgtatct tctgtcggca aaaatagccc caccaagcac
     5761 tctgatattt tcgggtgtgg ctatccagct tgatgtcttg gtgtcgaact gccccagttg
     5821 ctgtaatgct ctgtactgtg cttccgttaa aagtgctatg ccaatttctg ctgccatttg
     5881 ctgtgcactg ttttgaggtt tgtgttcctt tctcgcttcc agtgctttat ggtcatagca
     5941 aaggcttctt ctgcctttag gactttcttc cgaacagtca aagaaagtat attggccggt
     6001 atttttgtcg taggccacca catcgggttc tccaccagta tcttccatta aaaagaggat
     6061 attcaattta tccggttggg ctttcagttt gatttcaagc gattcccatt cgatgttttt
     6121 atgccgaatg ctatactttt caaaacgcat ttttaatgtg ctgatcagtg cagtacgttg
     6181 tttatctgta atcgctatac gtatttcatt tttcattcgt cagtatgttt tttcaattat
     6241 acaggtcaaa aaatttggaa aagctggcgt aagcagatgg tctgtttatt acttaatttc
     6301 cgtaaaaaaa tacaattggt attatgtggt ttgtccgtta tattttgggt caaaatctac
     6361 tatccattca aaaccaaatt tatctctgaa cataccaaaa tacgaacccc aaggactgtc
     6421 agcaatcggc atttcaactt gtccatcggc tgaaagccca ttaaacaaat gatcggcttc
     6481 ttctttactt tctgcgctga tggaaatttt gcttctgttt tcattttcat tatgtctgcc
     6541 cataaattcc ggtgtatcac tacccattaa gacattatgt ttgccaatag gcaaggcaat
     6601 atgcataatt ttatcggctt ctttttcggc attcgggttg tgtggatttg ccatgtcttt
     6661 ataacggaca agcatagcaa actctccgcc aaatatggat ttgtaaaagt tgaatgcttc
     6721 ttctgcattg ccattgaaat gaatgtacgg attaatttgt gccatcgctg aaattttatt
     6781 ttgtgattgt ttttgttacg catcagggat attcggttcc actaatcgtt ttaatttatc
     6841 taaagattct tgccaaccta aatagcacat ttcaacaggt attgcatcag gaatttcttc
     6901 ttgagtcgca attatttctg tgccacataa aactttttta agctctatgg tcgtaatcat
     6961 ttgccccggc aaattaggat catcgaactt gtcggtatat tttatttttt catccgggat
     7021 gatttctaca tattccccac caaaattatg cgagctacca gtggtgaaat ttgtaaatga
     7081 cattttatac gtaccgccca ctttaaaatc catgctatga actttgcaaa tgaaaccata
     7141 aggcggaatc caggaagcca ttgcatcggg gtctgaaaat gctctgaata ctttttcaac
     7201 cggagccgaa aaaacgcgat gtagttttac ggtattattt gccattactt aaatttagaa
     7261 gtcaaattta ctactattta atttgagttc gggataaaga tttcaaataa aaataatagt
     7321 ttatcttttt aaaataacaa tatgaactat tattaattat taacaagcat aattaaaaag
     7381 tcggatgata acatccgacc tttaatgaaa atagtattaa gtcggatgga aacatccgac
     7441 ttcacaggaa acatccaact tcaagttaca acgctgctat aaaatcttgc aatagttttt
     7501 ttagcttgtt tttatcgccg ttgctataag tgaaaaattg aaaatgatcg gcataatcat
     7561 ctgaatattc tgaccaaaaa caacttccgg ttttacattt tagtcttatg gtattgctac
     7621 tgtaatcttc tatataggta ttttgtttta gcttgctgat gctaatggta gatgcataat
     7681 tgagcccgga agaataatac ttaaagtaca catcattgtc ttttacttca atatctctgt
     7741 aagtctcttt gttataaatt tgcaaatatg cattcagctt ttttaatgca tcagcatatt
     7801 tatttgacgc tgatgtgctg ctagtagttc ccgatgattt agtagtttct tttttatctg
     7861 tttgtgtatt ggctaataca tcaaagtctt catcaatcca ataaaaatca tcctcatctt
     7921 cagtcttctt atttgaaccg gatttatttg cattttccaa tctttttttg gcttcttcat
     7981 cgcgtctgga tgttgatgta gaagttgttt ttgatgaaga tagattaggg tctgtgcatt
     8041 tttttaatgc ggtcaggaaa ttattcaata atttttccat ttgtgtcagc ttactgccgg
     8101 atgcattgaa gttaaaataa tcgtgataag aacctgtgat tgtagagtag atacatttac
     8161 tctcgccttt gcaagccaac ttcgcatagt tgtattgagt gttaatctca acatgatcga
     8221 tgtcttctat ttttgctttt gaatactgat tattttgata gcgattaatt atatatccat
     8281 ccgttacttc cattcctaca tacctgccat tgtcgagtgt taccattaaa ttattcagtt
     8341 cttgcaatgc cttatttgct ttttctatgt tttctgcctt actgcttgac gtagtttttg
     8401 atgatgatgt ggtcttattg gtgccactgt tcatattgtc tttattcgat tcgtacccat
     8461 taagaaattc tgaagactct gtatattttt cgctattatt agatgaggta ttttgttttt
     8521 tctttgacag ataagcatct ctgaagttgt ttaataagtc agccagttct ttgtaattgt
     8581 aagagccact ttttgtaaat tgggtataag gctcttttcc gtttggcttc caatctgtgg
     8641 aaatacagtc tcctgttttg catttaaaaa taactctgga atattcagcc tgtatgattg
     8701 caccttccat gtcttccatc ttgaatttgt tgtatttccc tgctttgaat ctatcataga
     8761 tgtagccgtc ttttacttcc caataaccgt aataaccatt atcaaatgtt ttcagatagt
     8821 catttagttt tttcaattcc gcttgataat tccctgaggt tgtttgctta gaactagaag
     8881 tggtgccact gctcatgttg tctttattcg atttgtactt gtccaaatca tcttgtaagt
     8941 taccatattc ttcagcctct ttattttttg cagttagatt tttatggtat gcttttacca
     9001 gattatttaa taaagaaata aattctcctg tattaaaatc ggtagcttgt gagaaggtaa
     9061 aagtattgta attcgcaccg gtaaatgttg aatatacaca cagatctgtg cctttgcatt
     9121 taagctctac ttttctgttt ttttccaatt cgtatgcata atctatctca ctcatcttt
//
LOCUS       DAOQII010000086         9141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4037_length_9141_cov_4.535769, whole genome shotgun sequence.
ACCESSION   DAOQII010000086 DAOQII010000000
VERSION     DAOQII010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4037_length_9141_cov_4.535769"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLF48_05630"
     CDS             complement(<1..934)
                     /locus_tag="PLF48_05630"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNF48840.1"
                     /translation="MESILPLLAQTQKASIQICNKTTSEKKALLASLATKLQENVEQI
                     MAANRLDLDKMDDSNPKKDRLLLNENRIKDLAESLISITQLADPSGKILSEYKAENGL
                     LIQKIAVPVGVVGVIYEARPNVTIDVAALCMYAGNAVVLRGGSDAEHTNSKLVQVIHD
                     TLDEHKWDKNGVQLLPVERKFVSQLLTAVRYVDIIIPRGSQQLIDFVRDNAKVPTIET
                     GAGVCHTYVEQSANLDMAAQIVVNAKTQRPSVCNALDTVLVDVAIAEQFLPKLLPGFL
                     PFEVEIFADYEAYDILKEANYPYLKKATDDNFGME"
     gene            1049..2053
                     /locus_tag="PLF48_05635"
     CDS             1049..2053
                     /locus_tag="PLF48_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF48841.1"
                     /translation="MKKFLKILVYTCLSLITILLIAVIIKLSFFGKIFDKHKAEEFLL
                     PSTNKKNIRLTFLGTSCFIIDYKGKQFVNDPYFSNQDFPSIIKGKTPLEKLSNFLPDS
                     LYNNISLISITHGHFDHCMDIGTFLQKNTAVIADSSVFYQLNQLIPDNTKKHQLHYQP
                     YANWIYSSDSTFRVFTILSHHGPHFGHTVLFNGSFHQPLASPPHYLWDWQLGSGNYSY
                     LVDVMDADSIVFRMAFLAGDIHDEEYKKIDSIYSEKRCDIFSLVFWKRKLNESQIDVG
                     EKHTKADIILLNHWNNFFSRQEKPLEEIRTSKILEILSDKKDKQTPCYIMLPFTSVAL
                     "
     gene            2104..3261
                     /locus_tag="PLF48_05640"
     CDS             2104..3261
                     /locus_tag="PLF48_05640"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNF48842.1"
                     /translation="MIKPSTLIDEFILHFEHPAETAFFSPGRVNLIGEHIDYNGGLVL
                     PCAISLGITALVRKNNTNTCRIFSVDFNEMIVVDISEQLTINQQKKMHWSDYVTATLR
                     VLQDKNSTLLGADILLASDLPVGSGLSSSAALSCLIAFIFNQKYYEENKVELALDAQT
                     AERNYVGVNCGIMDQFAVSNGKKNTAILLDCATLEAGYIPADFEDYQLVIINSNKSRL
                     LAESKYNERRSECDQALAAIRQYYPATDLCHAPEIALSYIEQDTLYARAKHCLLENQR
                     VIFAAQFLKDKNIEAFGRLLVESHISLEEDYEVSCTELDTIVHLATHFDDCAGARMTG
                     AGFGGCCIALVKKDKIQRFISYVGINYEHKTRLEADFYICAIADGVTETEL"
     gene            3355..4509
                     /locus_tag="PLF48_05645"
     CDS             3355..4509
                     /locus_tag="PLF48_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF48843.1"
                     /translation="MGYLASVDSMLLMPLINDVMKTFHISAQKSTLLISIYSLMAFIT
                     GILSAGIMDKFDRKKLLIYTFLGFTVGTAFCGFAYDFYSMLIARGIAGIFGGIVGGIA
                     TALISDLIPFERRATAISMMSLSFAVAAITGIPLALNLATEHNLQMPFKILALFSAVT
                     IFGLIQWIPPVNKHLEKVKTKKMEWAFAKNVLLDSNQLIALLLAFLLVFGHQVVITFI
                     VPYFENNIGFDENVKKIMYAVGGIATVITTPFIGKICDRKGNLPSFIVLVLLSFIPIW
                     LSTHMTKVSMYTALTICVLFFVTAGGRIIPAYTLMSAATTPEKRGGFMTMRSSFLELG
                     TAVAALVCGMIVQVAPDGKVLHFNQVGLISIISGILALYLASRIKIVSRL"
     gene            4584..4817
                     /locus_tag="PLF48_05650"
     CDS             4584..4817
                     /locus_tag="PLF48_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48844.1"
                     /translation="MAKMTKVFGAIFLALGTLYTTVSIFLYLNSSAYLDYNTGKKMTM
                     SQAGVFYYLFFSVLLLIIGYVLAKDKVLGNNKH"
     gene            complement(5074..5925)
                     /locus_tag="PLF48_05655"
     CDS             complement(5074..5925)
                     /locus_tag="PLF48_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48845.1"
                     /translation="MIYLLLSIICSTSLVVILRLFDKWKIKTEYGIVFNYLVCVITGL
                     IAMDYKSMLQQVPSWNGWWICLLLGLGFILVFRIVGKSTIEAGVASTTIAFKLSFIIP
                     VIVAILFYGDALTANKIIGIILAIAAVILITYSGESQRAKISYLPLIIFIGSGITDSV
                     FNFIQRDYTPPGFDHIVTITVFLGAFLSGFILFGFKKEMYQWKNVLGGIVLGIPNYFS
                     LFFLLKALKFTGWTPSILFPVNNLGIVMLSAVFGIVLFREHFNRNKIIGFLLALLSIS
                     VIAFWNS"
     gene            6076..7029
                     /locus_tag="PLF48_05660"
     CDS             6076..7029
                     /locus_tag="PLF48_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HNF48846.1"
                     /translation="MKNILLNIFLVSFFALSCKQLVKNQNQIVENEIDSIQSVSENKT
                     QRPVTTNTGVLSKNDLIGFWVGYFRNAKDEDYDKGVYADETLSWTRENKINISIDEIS
                     GNAVKGHSIVAGNNRPFAGTCSEGNNAYSFIVSEPGDNKYDGKFSFSIQKNDSMLTGT
                     WKSYKKIDIQERKYELVKKIFNYNPEQMLERAREYGNWEKRKKVKIEDAEYDEFYDEF
                     SAATNKIYQMNASKNLLTKKDVENLKKGDLLIIRNTIYARHGYSFKNRPLRVFFDAQS
                     WYIPVHTDIVSDLTDIEKKNIELLLKYEKNAKEYYDTFGRG"
     gene            6992..7696
                     /locus_tag="PLF48_05665"
     CDS             6992..7696
                     /locus_tag="PLF48_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNF48847.1"
                     /translation="MRKSIMILLGVVNLLIACQNKNVSVQHETIVDIEHSSDTSANTF
                     SERKDDIINIPSSFCKVNLDSQKIINKMAYATSENFTKQKLYPCASCYLRNEVAIALS
                     KAQHGAQKMGLVLVVFDCYRPYSIQKKMFEIVGNSNYVAHPGKGSMHNKGCAVDVSLA
                     EADGKLLDMGTEFDDFTEAAHFANTAINRLEHRNRKQLRDIMTSVGFLPYNNEWWHFN
                     YLITDSKTEDFVWSCP"
     gene            7693..8328
                     /locus_tag="PLF48_05670"
     CDS             7693..8328
                     /locus_tag="PLF48_05670"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNF48848.1"
                     /translation="MNKTKISNIVVSVVIYGTLSFFIYLMALITLQYIPINFNAAFLV
                     TKQEEIKFVHYQIAFFTHVYSAIFVLFFGMFQFSGFVRKRYSNIHRVLGKLYVGILLF
                     LAAPSGFIIGWYGNGGISSVISFCMLAILWFYFTLIAYLSIRKGLYKKHEHYMILSYA
                     LTLSAVSLRLFKMFIANALALPPMDTYRIVVWLGWVFNLAVALLIIAYKEK"
     gene            complement(8352..>9141)
                     /gene="ribD"
                     /locus_tag="PLF48_05675"
     CDS             complement(8352..>9141)
                     /gene="ribD"
                     /locus_tag="PLF48_05675"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNF48849.1"
                     /translation="VSLEPCSHFGKTPPCVDLILQHKIPRVFISVQDIFEKVNGSGIE
                     KLKQHGVEVITDVLKEEGENLIKHFLHYHRFKRPYITLKWAQSADGYIGSIGERIGIS
                     NKESCYFLHQLRVKHQAILVGRNTVLNDNPSLTVRHANGINPLRIVLGSPDGIPGNYA
                     VFNESSPTIFISKNDSKNLSSLMRLLYEKQIISVLVEGGAQILNSFIREQLWDEAFVY
                     YSNKNLQSALPDKISAPTIHGKIAEIIKFEEDVVINYIPANNIS"
BASE COUNT         2931 a         1637 c         1646 g         2927 t
ORIGIN      
        1 attccatacc gaaattatca tctgttgcct ttttcaaata aggataattt gcttctttca
       61 gtatatcgta tgcttcataa tccgcaaaaa tttccacttc aaacggaaga aatcccggca
      121 acaattttgg taaaaactgt tcggcaattg caacatcaac caacaccgta tctaatgcat
      181 tgcacaccga tggacgttgt gttttagcat taaccactat ttgagcagcc atatccagat
      241 ttgccgattg ctctacatac gtatgacaaa cgcctgcacc tgtttcgata gtaggtactt
      301 tagcattatc ccgcacaaaa tcaattaact gttgtgaacc acgaggaata atgatgtcca
      361 catatcgaac agccgtcaat aattgactga caaatttgcg ttctaccggc aaaagctgaa
      421 caccattttt atcccattta tgctcatcta aagtatcatg aataacttgt accaatttac
      481 tgttcgtatg ttctgcatcg ctgccgccac gcaataccac ggcattacct gcatacatac
      541 acaaagcagc aacatcaata gtaacattag gacgtgcttc atagattaca ccgaccacac
      601 caacaggcac ggctattttt tgtatcaaca aaccgttttc tgctttatat tcactcaaaa
      661 ttttgccgga tggatctgcc aactgagtga ttgaaatcag gctttcagcc aaatctttta
      721 ttctgttttc attcagtaat aaacggtctt ttttaggatt gctgtcgtcc atcttatcta
      781 aatcgaggcg attggctgcc attatttgtt caacattttc ctgtaatttt gtggcaagtg
      841 aggcaagcaa tgcctttttt tctgaagtag ttttattaca aatttgtatc gatgcttttt
      901 gtgtttgagc taataatggg agtatagatt ccatacaatt tattttaagt aaaatagtaa
      961 aagcctgcta aaacaattca ataatgcata ttactaaaag tatattaaaa cttttttcct
     1021 gtacagtttc ttatctttga atatagacat gaagaaattt ctaaagatat tagtttacac
     1081 atgcttgagt ttgataacta ttttgctcat tgctgtaatc atcaaactca gttttttcgg
     1141 aaaaattttt gataagcata aagctgaaga atttttatta ccgtctacaa ataagaaaaa
     1201 tatcagactt acttttttgg gtacttcatg ttttatcatc gattacaaag gaaagcaatt
     1261 tgtaaacgac ccttatttta gcaatcagga ttttccatct atcattaaag gcaaaacgcc
     1321 tttggaaaaa ctgtcaaatt ttctaccaga ttccttgtac aacaatattt cacttatcag
     1381 tatcactcat ggtcattttg accactgtat ggatattggt acttttttac aaaaaaacac
     1441 agcagtcatt gcagactcaa gcgtttttta ccagctaaat cagcttatac ccgataatac
     1501 gaaaaaacat caattgcatt atcagcccta tgctaattgg atctattcat cagacagtac
     1561 attcagagta tttaccatac tttcgcatca tggtccacat tttggacata cggtgctatt
     1621 caacggctcg tttcatcagc cacttgcttc acctccgcat tatctatggg attggcaact
     1681 gggcagtggc aattattctt atctggtgga tgtaatggac gcagatagta ttgtattcag
     1741 aatggcattt ttagccggag atattcatga tgaagaatac aaaaagatag actccattta
     1801 ttcggaaaaa agatgcgata tattttcttt ggttttttgg aaacgtaagc taaacgaatc
     1861 tcaaattgat gtgggtgaaa aacatacaaa agctgatatc attttactta atcactggaa
     1921 caatttcttc agcagacaag aaaagccatt ggaagaaatc agaacatcta aaatattgga
     1981 aatactaagc gataaaaaag acaaacaaac tccttgttat attatgttac cttttaccag
     2041 tgtagcactc taaaaattgt tcaagcattt ttttacatta gatgattgtt tatttttgca
     2101 accgtgataa aaccttccac actaattgac gaatttattc tgcattttga gcatcctgcc
     2161 gaaactgcat ttttttctcc gggaagggtc aacttaatcg gagaacatat tgactacaat
     2221 ggcggtttgg tattgccttg tgcaatttca ttgggtatta cagccttagt tcgcaagaac
     2281 aatactaata cgtgtcgtat tttttcggtt gattttaatg aaatgattgt tgttgacatt
     2341 tcggagcagc taactattaa tcaacaaaaa aagatgcatt ggtcagatta tgttacagca
     2401 actttacggg ttctgcagga taaaaacagt acacttttgg gtgcagatat tttgttagcc
     2461 tctgatctac cggttggttc gggcttgtct tcatcggcgg ctttatcttg tttaatcgca
     2521 tttattttca atcaaaaata ttacgaagaa aacaaggtag aacttgcact ggatgctcaa
     2581 acagctgagc gcaactatgt aggagtcaat tgcggcatca tggatcaatt tgctgtatct
     2641 aacggcaaaa aaaacacggc tatactgtta gattgtgcaa ctctggaggc aggttatatt
     2701 ccggctgatt ttgaagacta ccaacttgtc atcatcaatt ctaataaatc gagattgctg
     2761 gctgaatcaa aatacaatga acgacgcagc gaatgcgatc aggcacttgc agccatacgt
     2821 cagtattatc cggccactga tttgtgtcat gctccggaaa tcgcattaag ctatattgaa
     2881 caagacacac tatatgccag agcaaaacat tgtctgctgg aaaatcaaag agtaatattt
     2941 gccgcacaat ttttgaaaga taaaaacatt gaagcatttg gcagactatt agtagaatct
     3001 cacatttcac tggaagaaga ttatgaagta agctgtaccg aactggacac tattgtacac
     3061 ttagctactc actttgatga ttgtgccggt gcccgtatga ccggtgccgg ttttggcggt
     3121 tgttgtattg cattagttaa aaaagataaa atacaacgat ttatttcgta tgttggcata
     3181 aattatgagc ataaaacaag attagaagct gatttttaca tttgtgcgat tgcagatggc
     3241 gtaaccgaaa cagaacttta agttatagaa acgatagaca gcatatcatt agagaaagat
     3301 gaactgccac atggcaaaag gaatgaatat ctcatcgtta tcctgcttgc ctggataggc
     3361 tatttagcct ctgtagactc catgttgctg atgcctttga taaatgatgt gatgaaaacc
     3421 ttccatatct cggcacaaaa atctacctta ttgatttcta tttattcgct aatggctttt
     3481 attacaggca ttctgtcggc aggcatcatg gataagttcg accggaaaaa actgttaatt
     3541 tacacctttt taggttttac tgtaggcact gccttttgcg gatttgccta tgatttttat
     3601 tccatgctga ttgcacgtgg aattgccggc attttcggtg gcatcgtggg tggcatcgcc
     3661 acggcattga tcagtgattt aattccattc gaacgtcggg caaccgccat cagtatgatg
     3721 tcgctctctt ttgcagtagc tgccattacc ggaatcccgc tcgcactcaa tctggcaaca
     3781 gaacataatt tacaaatgcc ctttaaaata ctggccttat tttccgcagt taccattttt
     3841 ggactcatac agtggattcc tcctgtaaac aaacacttgg aaaaggttaa aacaaaaaaa
     3901 atggaatggg catttgccaa aaatgtattg ctcgacagca atcaactaat tgcattactt
     3961 ttggcttttc tgcttgtttt cgggcatcag gtcgtgatta catttattgt gccctatttt
     4021 gaaaacaata taggttttga tgaaaacgtt aaaaaaatca tgtatgccgt tggtggaata
     4081 gccactgtaa taactacccc atttattggc aaaatatgtg accgcaaggg caatttgcct
     4141 tcctttatcg tgctggtttt gctttctttt attcccattt ggttgagtac acacatgaca
     4201 aaagtatcaa tgtataccgc acttacaata tgcgtcttgt tttttgtaac agcaggaggc
     4261 agaataattc ctgcatatac cttgatgagt gcagctacca caccggaaaa acgcggtggt
     4321 ttcatgacca tgcgttcttc atttttggaa ttgggaacgg cagttgcagc tctcgtttgt
     4381 ggtatgattg ttcaagttgc acccgacggt aaggttttgc actttaacca agtaggtcta
     4441 atttccatta tttcaggaat tcttgcacta tatctggctt cccgtattaa aattgtcagc
     4501 cgattgtaag gcataataaa aatttattac tgcaattttg ctagatttgc aaaagaaatg
     4561 ttaattttag aaaaaatttc tttatggcaa aaatgactaa agttttcggt gcaattttct
     4621 tggcattggg tacgttatac actacagttt ctatttttct ttatttaaat tcatctgcct
     4681 atttagatta taataccgga aaaaaaatga caatgtctca agcgggagta ttctattatt
     4741 tatttttctc ggttttatta ttaataatcg gttatgtatt agcaaaagat aaagtgttgg
     4801 gtaacaataa acactaatcg tatttattga cccgattttt tgttttcatg gttataagtt
     4861 ttgtccccgc gctagcaata gctcggggtt tttgtttgtg tattattatt gccaaaccta
     4921 aaattatgaa ttcgggataa taatagttga ttctttttta tctcgaacct cctccacctc
     4981 ctttttattt ccacttccta attaagttgg aggaaaaatg agaaatcaac tgttttttta
     5041 ccttattttt cttaagccga actcaagtta aaattatgaa ttccaaaatg caatgacaga
     5101 aatacttaat aatgccagca ggaaaccgat tattttatta cggttaaaat gttctctgaa
     5161 taagacgata ccaaatactg cagacaacat aactataccc aaattattta caggaaataa
     5221 gatggaaggt gtccatcctg tgaatttaag tgcttttagc agaaaaaata aagaaaaata
     5281 attgggaatg ccgagtacga ttccgccaag tacatttttc cattgataca tttctttttt
     5341 aaatccgaat aaaataaaac cggataaaaa tgcgcctaaa aatacagtaa ttgtcacaat
     5401 gtgatcgaaa ccgggaggtg tataatcacg ctgaataaaa ttaaacaccg aatcagtgat
     5461 accgctgccg ataaagatga ttaaaggtaa atacgaaatc tttgcacgct ggctctcgcc
     5521 cgaataggta atcaaaataa cagcagcgat tgccagtata atgccgatga ttttattggc
     5581 tgtcagggca tctccataaa ataaaatggc aacaataacc ggaatgataa acgaaagttt
     5641 aaaagcaatt gttgtactcg caactcccgc ttcaatagta gacttcccca caatccggaa
     5701 gaccaaaata aaacccaatc ctagcagcaa acaaatccac cagccattcc aggaaggcac
     5761 ttgttgtagc atggatttat agtccattgc gataagacct gtaataacac aaaccagata
     5821 attaaacacg atgccatatt cggtttttat tttccattta tcgaatagtc gtaaaattac
     5881 cactaaagag gtagaacaaa taatgctgag gagtaagtaa atcataatag gatttctaaa
     5941 gttagaatag tttttcaatt ttaagctaat gcagattaaa tattcagcaa aaaacttttt
     6001 tattactcaa gtttgatttt tgaaaatctg cttcaactga atattttgtg ttatttttag
     6061 acttaaacat aaacaatgaa aaatattctt ttaaacatct ttttagtcag cttcttcgct
     6121 ttaagttgca aacaactcgt taaaaaccaa aatcagatag ttgaaaatga aattgattca
     6181 attcaatcgg tctcagaaaa caagactcaa cgtcctgtta ccacgaatac aggcgtactt
     6241 agtaaaaatg atttgattgg attctgggtg ggttatttca ggaatgcaaa agatgaagat
     6301 tacgataaag gagtatatgc cgatgagacg ttgagttgga cgcgtgaaaa taaaattaat
     6361 atatctattg atgaaatttc tggaaatgcg gtgaaaggac atagtattgt cgccggtaat
     6421 aaccgacctt ttgcaggtac gtgttcggaa ggaaataatg catattcctt tatagtatca
     6481 gagcctggcg acaataaata tgatggtaaa ttttcgtttt ctatccaaaa aaatgattcg
     6541 atgctaacag gcacatggaa gtcttataaa aagatagata ttcaagaacg caagtatgaa
     6601 ttagttaaga aaatatttaa ctataatccg gaacagatgt tggaaagagc gcgagaatat
     6661 ggcaactggg aaaaacgcaa gaaagtaaaa attgaagatg ctgaatatga tgaattttat
     6721 gatgaatttt ctgccgctac aaataaaatt taccagatga atgcgtctaa aaatttgctg
     6781 acaaaaaagg atgtagaaaa cttgaaaaaa ggagatttgc ttattataag aaataccatt
     6841 tatgcccgac atggctactc ttttaaaaac agaccattgc gggtattttt tgatgctcaa
     6901 tcttggtata ttcctgtgca tacggatatt gtatctgacc ttacagatat tgagaagaaa
     6961 aatattgaac tgcttttgaa atacgaaaag aatgcgaaag agtattatga tacttttggg
     7021 cgtggttaat ttactgattg cctgccagaa caaaaatgtt tctgttcagc atgaaactat
     7081 tgtggatata gaacattctt cagatacatc tgcaaataca ttttctgaaa gaaaagatga
     7141 tattattaat attccatctt cattttgcaa agtgaattta gacagtcaaa aaataataaa
     7201 taaaatggcg tatgccacat cagaaaattt taccaaacaa aagttgtacc catgtgcttc
     7261 gtgttattta agaaatgaag tagccattgc gttaagcaaa gctcaacacg gagctcaaaa
     7321 aatgggattg gtattggttg tttttgattg ctaccgaccg tattctattc agaaaaaaat
     7381 gtttgagata gtcggcaatt ctaactatgt agcgcatccc ggaaaaggtt ctatgcacaa
     7441 taaaggttgc gcagtagatg taagtcttgc ggaggctgat ggcaagctgc tggatatggg
     7501 aactgaattt gatgatttta ccgaagcggc gcattttgcg aatacggcaa ttaacaggtt
     7561 ggaacacaga aacagaaaac aattaagaga cattatgacc agtgtaggat ttctgcctta
     7621 taataatgag tggtggcatt tcaactatct tattaccgat tctaaaacag aagattttgt
     7681 atggtcatgc ccatgaacaa aaccaagata tcaaacatag ttgtgtctgt tgtaatctat
     7741 ggaacattgt cgttttttat ctatttaatg gcattgatta ccttgcaata tattcccatc
     7801 aattttaatg ccgcgttttt agtaaccaaa caagaagaaa tcaaatttgt acactaccaa
     7861 atcgcatttt ttacgcatgt atactcggca atttttgtgt tgtttttcgg catgtttcag
     7921 ttttcaggat ttgtacgaaa aagatacagt aacattcacc gggtattggg aaaattatat
     7981 gtaggcatac ttttattttt ggcagcgcca tccgggttca ttatcggatg gtatggtaat
     8041 ggcggaatat cgtctgttat atcattttgt atgctcgcta tactgtggtt ttattttaca
     8101 ctaatagcat atctgagcat cagaaaaggg ctatataaaa aacatgaaca ttacatgata
     8161 cttagctatg cgctcactct ttctgccgta agtctgaggc tgtttaaaat gtttattgca
     8221 aatgcactcg cactaccacc gatggatact tacagaattg tagtatggtt gggttgggtg
     8281 tttaatttag cagttgcgct gttaataata gcttataaag agaaataaat aaactggata
     8341 attatcctcg gttatgagat gttgttggca ggaatataat ttatcaccac gtcttcctcg
     8401 aactttataa tctctgcaat ttttccatga attgtcggtg cacttatttt atcgggtaat
     8461 gctgactgta aatttttatt ggaataataa acaaatgctt catcccaaag ctgttctcga
     8521 ataaacgagt ttaaaatctg cgcgccgcct tccaccagca cggagataat ttgtttttca
     8581 tacaaaaggc gcatcaggga agacagattt ttagaatcat ttttggaaat aaatattgtt
     8641 ggcgatgatt cattgaaaac cgcataattt cccggaattc catccggact gcccaacacg
     8701 attctcaacg gatttatgcc gttggcatgg cgtaccgtca aacttggatt gtcgttcaaa
     8761 accgtattcc ttcccactaa aatagcctga tgcttcacac gcaactgatg caaaaaatag
     8821 cacgattctt tattacttat acctattctt tcaccaattg aaccaatata tccatcggca
     8881 gattgcgccc atttcagggt gatataagga cgcttaaatc tatgataatg caaaaaatgc
     8941 ttaatcaggt tttcgccttc ttctttaagt acgtctgtta taacttctac accatgttgc
     9001 tttaactttt cgataccgct accatttact ttttcaaaaa tatcctgaac agaaatgaaa
     9061 actctcggaa ttttatgttg taagatcaaa tcaacacaag gcggtgtctt tccgaaatgt
     9121 gagcaaggtt ctaagctgac a
//
LOCUS       DAOQII010000087         9133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4044_length_9133_cov_4.332122, whole genome shotgun sequence.
ACCESSION   DAOQII010000087 DAOQII010000000
VERSION     DAOQII010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4044_length_9133_cov_4.332122"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /gene="recJ"
                     /locus_tag="PLF48_05680"
     CDS             complement(<1..1441)
                     /gene="recJ"
                     /locus_tag="PLF48_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNF48850.1"
                     /translation="MITQKRWILSDADADTVAQLQQSLKIHPVICKLLAIRGIDTYDK
                     AKAFFRPSLADLHDPFLMKDMDKAIERIEQAIASEEKIMIYGDYDVDGTTAVALVYSF
                     FREFYNHLLYYIPNRYTEGYGISFDGMDFAFENQVKLIISLDCGIKANDKIAYAKEKG
                     MDFIVCDHHLPGEELPDAVAILDPKRKDCTYPFDELSGCGIGFKLIQAFCIKNNIAIE
                     KAYQQLDLVATSIASDLVQITGENRILAKFGMDKVNENPRAGFKALLNELKIDKKLEI
                     SDLVFIIGPRINAAGRIAHGSEAVELLIENDLNIALEKTKKVQQNNDERKNLDKDITE
                     EAFSMLESSVEYADRKSTVLFQSHWHKGVIGIVASRLIEKFHRPTIVLAASNGKAVGS
                     GRSVPGFDLHDAIETCSEYLVQFGGHKYAAGLTIELDNIDAFSAAFEKIVSERITSEQ
                     LIPQIEIDAEIELEDINEKFYNIVEQMAPF"
     gene            1597..1914
                     /locus_tag="PLF48_05685"
     CDS             1597..1914
                     /locus_tag="PLF48_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48851.1"
                     /translation="MLTKFIYMSVGTAAVSIEKFKELLEDLIQNDAFIEDEGKRIVDA
                     LLHDMRENLDHYRQNAQLKWDDFLENSGFSKIRHAKSDMEHFVKDVKENPFALLTRWE
                     KEN"
     gene            complement(1947..2603)
                     /locus_tag="PLF48_05690"
     CDS             complement(1947..2603)
                     /locus_tag="PLF48_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF48852.1"
                     /translation="MQEIKLVIVDDHQIFLDGIVSLLEDEPGIKITGTAHNGSEALSL
                     IKQTKPDVVLMDINMPVMDGIEATKQLKQSQQDVKVLMLTMHSEPRFIKECLEIGAKG
                     YVLKNISKDELLKAIETVYQDKPYLHPEAQEKLISSMSNSEDKEDDRNYDELAALITQ
                     RELEILQLIALGLTSQDIAQKLFISKNTVETHRKNMLAKLNVNNTAALLKIAYKKGLV
                     "
     gene            complement(2634..2996)
                     /locus_tag="PLF48_05695"
     CDS             complement(2634..2996)
                     /locus_tag="PLF48_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48853.1"
                     /translation="MIVWVSAWFIKYFTPKNIGGISIFPFIIVENQDIKKDKIFINHE
                     KIHIWQQLEMLIILFYIWYGLEFLILWLKYKNGFKAYRNISFEQEAYAHELDLNYLKN
                     RKLFSFIKYLKIGSKIDE"
     gene            3072..4256
                     /locus_tag="PLF48_05700"
     CDS             3072..4256
                     /locus_tag="PLF48_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNF48854.1"
                     /translation="MASRKFDIIIYGCTGFTGKLVAQYFADHVDITKIKWGIAGRNIN
                     KLEALKAKFILANKANYDIGVFECDAEDMQSLQEVSGSTKILISTVGPYALYGENTVA
                     ACVTTNTHYCDITGEPAFVQNIMKKFGAEAGAKGLYIVNCCGFDSIPADAGAFFTAMQ
                     LPANEQKTINGFVSTNASFSGGTYASAINAFSEMGKNISLFGTKKPEHKSTNKKQRAK
                     ATTLPYFEKQINKWALPMPVIDLWIVQRSAQLRPSVYGSDFYYAQFMAIRKLQQVISL
                     VGSVGAVIIGAQIPPVKNMLLNLKKSGEGPSQEQRDKSTFQVVFIGKSETMQIKTKVS
                     GGDPGYTETSKMLSETALTLLENLGNIDTAPGITTPIGALGNKLIDKLISKGIRFEVI
                     SM"
     gene            4393..5361
                     /gene="hemB"
                     /locus_tag="PLF48_05705"
     CDS             4393..5361
                     /gene="hemB"
                     /locus_tag="PLF48_05705"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNF48855.1"
                     /translation="MLHRPRRNRKSAAIRGMVQEIHLQSSHLVQPLFLVDGKNKRNPI
                     KSLPSQAQLSIDVAIKEIEACMKLGVQNFILFPAVENALKDKKASYSYSPKNFYLKAI
                     KEFKKEFPECCLISDVAMDPYSSDGHDGYVENGKIINDKTLPILAKMSLAQAQAGIDI
                     LGPSDMMDGRVGYIRELLDADNFQEVGIMSYSAKYASAFYGPFRDALDSAPKSGDKKT
                     YQMNFCNKKEALLEAELDYNEGADFLMVKPATCYLDIIQDLKQNFPVPIAAYHVSGEY
                     AMLKAAAQNGWLDFNKTLDETLTAIRRAGSDVIITYGAKEWAEAVG"
     gene            5375..6382
                     /locus_tag="PLF48_05710"
     CDS             5375..6382
                     /locus_tag="PLF48_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF48856.1"
                     /translation="MINILILGGGRLIGQNLLAKLAKNKPYAITTFSRGMTNSSQDFS
                     VNRLFGDRRKTEDLHQINDRDWDIVIDLSCYWPDALKQQLEQQKGKIGKYIFISTSSL
                     FDFDENNPHFISEDEPTVSCSPEQIADETGASYNARKAECERVLQQFPQLNYVMLRPG
                     LILGKHDYTQRLYYWLYRISKCQAYLQPEKFNNRIAFTDVDDLCAMIDFFIHTQQQHT
                     IYHAISFHATLAEIVAVTQQKLNKKPEVVIATDEFLDKHQIHEWVDLPLWLKNEFMLI
                     DNSRIKQAIDFPLSTLDATISKLLQYYEQDCHWPLPNTSPTSLSLEREQILINELINL
                     S"
     gene            complement(6528..7643)
                     /locus_tag="PLF48_05715"
     CDS             complement(6528..7643)
                     /locus_tag="PLF48_05715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF48857.1"
                     /translation="MNPLNIVQVIDVFDDARNGAVISTQRFTTALRQKGHQVKVISTG
                     KKSQDKIIMPEFYPPVPFVKNVMQKMKTQFAFPDEKKINSVIETCDIVHVQFPFWLSM
                     KCIEIAQQYKKPIVSTFHVQAEQIAYNINIKNKWLIKKINQFFIEQIYNKSDVVICPS
                     IFAQDELKRYGLKTNSVVISNGIMKENFLMSNRNQGSPYFHILTVGRNAVEKRQDMLI
                     EAISKCKNKKQIKLSIIGDGPIREKLTTLANNKLPNQVEFLYVSKDEITRYYQEADLY
                     VHCAGVEVESMSTMEAMAAGLPMIIADAPLSAAKQFALNSDFLFKTTTELTHKIDYYF
                     EHSNELQQAAEKYALQAKKYAFDISVNQLTELYYSLQ"
     gene            7787..>9133
                     /locus_tag="PLF48_05720"
     CDS             7787..>9133
                     /locus_tag="PLF48_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNF48858.1"
                     /translation="MKNAIFAMGLSLFYLQVSAVTINNSKVSEVTVYRNFAKETRLAS
                     DNLNEGVQEVIISNVSPFMDENTIQIATKGNVKILSVSSRNNFLSDKKKTDVSQKLSD
                     SLKLLNNENDWIAEQIAAYQGELEVLDNNKKLANDKLLFKSTDVKDIADFYIVKALEI
                     RRKLLDLVHEQTANTEIITRITQQINDIGNQQKSVKEIVLKVNALSATAATFKCSYVV
                     SSAYWMPVYDIRAKNLTEPLQVDCKAKVVQNTGMDWKDIKLKLSTANPASNQSRPVLY
                     PLYVDFFQPDYYYKKMESKISGVVAAPQMMQNSMDIARSGMDKEEFTDGLRADDNGVT
                     LLEGETTQEYDIQTLQDIESDNQAHIVGIQEIKMQAVYNYHAVPKLDPTAYLLARVTD
                     WAKYNLLAGEANIFFDDSYVGKSYLNPNVSADTLLISLGRDEKINIKRIKLNDYTST"
BASE COUNT         2955 a         1652 c         1670 g         2856 t
ORIGIN      
        1 caaatggtgc catttgctcc acaatattat agaatttttc attgatatct tccagttcta
       61 tttctgcatc tatttctatt tgcgggatga gttgttctga agtaatacgc tcgcttacaa
      121 tcttttcaaa tgcggcagaa aatgcatcga tattatctaa ttctatcgtt aaccccgccg
      181 catatttatg cccgccaaac tgtactaaat attcgctgca agtttcaata gcatcatgta
      241 aatcaaaacc gggaaccgaa cgtcctgaac ctacagcttt gccattagat gcagctaata
      301 caatagtagg gcgatgaaat ttttcaatta agcgagatgc cacgatacct ataacgcctt
      361 tatgccaatg tgattgaaac aagactgtag attttctatc cgcatattct actgaacttt
      421 ccagcattga gaatgcttcc tcagtaatat ccttatctaa gtttttgcgt tcgtcattgt
      481 tttgttgcac cttcttagtt ttttccaaag caatattcaa atcattttca ataagcagtt
      541 caaccgcctc tgaaccgtgt gcaattcgtc cggctgcatt aattcgagga ccaataataa
      601 atactaaatc tgaaatctcc agtttcttgt caattttcag ttcgttcaat aatgctttaa
      661 atccggctct cggattttca ttgactttat ccattccgaa tttagcaagt atgcggtttt
      721 ctcctgtaat ctgaactaaa tcagaggcta tgctcgtcgc tactaaatcc aattgctgat
      781 atgccttttc gatagctata ttatttttga tgcaaaaagc ctgtatcaat ttaaatccta
      841 ttccacagcc actaagttcg tcgaatggat aagtgcaatc tttgcgtttg gggtctaaaa
      901 tagcaacggc atccggcaat tcctcaccgg gcaaatggtg atcacagaca ataaaatcca
      961 tgcctttttc tttagcatag gcaattttat catttgcttt tatcccgcag tctagagaga
     1021 taatcagttt aacctgattt tcaaatgcaa aatccatacc gtcaaaagaa attccgtaac
     1081 cttctgtata acgattgggt atgtaataaa ggagatgatt ataaaattcg cggaaaaaag
     1141 agtaaaccaa tgctactgct gttgtaccat cgacatcata atctccgtaa atcattatct
     1201 tttcttcgga ggcaatagct tgttctattc tttcaatcgc tttatccata tctttcatca
     1261 aaaacggatc gtgtaaatca gctaaagatg ggcgaaaaaa tgctttagct ttgtcataag
     1321 tatcaatacc tcgtatggct aataatttac aaatgacagg atgaatttta agcgattgct
     1381 gcaattgggc aaccgtatcg gcatcagcat ccgacaatat ccatcttttt tgcgtaatca
     1441 tattttctgc aaaatagtca gaaatatcgt tttatttgta attaatttaa aatttaacgg
     1501 tattgccagc cttaatttta ttttttactt aatattactt tctttttctg ctaaaatagg
     1561 tagcataatt ttgtttcgat atcaaatcta aaaatcatgc tgacaaaatt tatctatatg
     1621 agtgtaggaa cggcagccgt ttctatcgaa aagtttaaag aattactaga ggatttgatt
     1681 caaaatgatg catttatcga agatgaaggc aaacgtattg ttgatgccct tttgcatgac
     1741 atgcgtgaaa atctggatca ttatcgccaa aatgctcagt taaaatggga cgatttcttg
     1801 gaaaatagcg gattttcaaa aatcagacat gccaaatcag atatggaaca ttttgtaaaa
     1861 gatgtaaaag aaaatccttt tgctttacta acccgttggg aaaaagaaaa ctgaaacctg
     1921 cttctttcaa atcaaacaaa tcaagcttat accaatcctt ttttataagc tattttcagc
     1981 aacgcagccg tattgtttac gtttaatttg gcaagcatat ttttgcggtg tgtttccacc
     2041 gtgtttttac tgataaatag tttttgggct atatcctgag aagttaaccc taaagctata
     2101 agttgcaaaa tttccaactc tcgctgtgtt atcaaagcag ccaactcatc atagttacgg
     2161 tcatcctctt tatcctccga gttggacata gagctgatta atttttcctg tgcttcagga
     2221 tgcagataag gtttatcctg gtaaactgtt tcaatcgctt tcagcaattc atctttggaa
     2281 atatttttga gtacatagcc ttttgcccct atctccaggc attcttttat aaatctgggc
     2341 tctgagtgca tcgtcagcat cagcactttc acatcctgtt gagattgttt cagttgtttc
     2401 gtagcttcaa tgccatccat caccggcata ttaatatcca ttaataccac atccggtttg
     2461 gtttgcttaa tcaaactcaa ggcctcgctg ccgttatggg ctgtacctgt aatttttatt
     2521 cccggctcat cctccaaaag tgacacaatt ccatctaaaa atatctgatg atcgtcaact
     2581 ataactaatt taatttcttg catatgtatt tttgttcgtg tgcaaaggta attctattca
     2641 tcaattttcg agccgatttt aagatatttt ataaatgaga agagttttct gtttttcaga
     2701 tagtttaaat ctagctcatg tgcatatgcc tcctgttcaa aagagatatt tctgtatgct
     2761 ttaaatccgt ttttatattt cagccataat atcaaaaatt ccaatccgta ccagatataa
     2821 aataaaataa taagcatttc caattgttgc cagatatgta ttttctcatg attgataaat
     2881 attttgtctt tttttatgtc ttgattttct acaatgataa atgggaaaat actgataccg
     2941 ccgatattct tcggtgtaaa atatttgata aaccaagcac ttacccagac aatcatgtaa
     3001 ttaaatttca attaaaaata ctaaaacctt agcaggattg gatatgttgc ttaattttat
     3061 aaaaaaataa aatggcctct cgcaaattcg acatcattat ttacggctgt acgggcttta
     3121 ccggaaaact ggtagcacaa tattttgccg accacgtgga cattacaaaa ataaaatggg
     3181 gcattgccgg aagaaatata aataaactgg aagccttaaa agcaaaattt attttggcca
     3241 ataaagctaa ttatgatatt ggagtgtttg aatgtgatgc agaagatatg caatcgttgc
     3301 aggaagtgag tggaagtacc aaaattctga tttctacggt agggccatat gcgttgtatg
     3361 gcgaaaatac agttgccgct tgtgtaacca caaatactca ttattgcgat atcacgggtg
     3421 agcctgcttt tgtacaaaat ataatgaaaa aatttggtgc tgaagccgga gcaaaagggc
     3481 tttacatcgt caattgttgt gggtttgaca gtattccagc tgatgcaggt gcattcttta
     3541 cagccatgca attacctgcc aatgagcaaa aaacgataaa cggatttgtc agtacgaatg
     3601 ccagcttttc cggtggaact tatgcttctg ccatcaatgc tttttcggaa atgggaaaaa
     3661 acatttcctt attcggaaca aaaaaaccag aacataaatc cactaataaa aaacaaagag
     3721 ctaaagcaac aacacttccc tattttgaaa agcaaatcaa caaatgggca ttgcctatgc
     3781 ccgtgattga cctatggata gtgcaacgaa gtgcacaact cagaccttcg gtttatggca
     3841 gtgattttta ctatgcgcag tttatggcta tccgaaaatt acagcaagta attagtttag
     3901 tgggcagcgt gggcgccgta attataggtg cgcaaattcc acccgtaaaa aacatgttgc
     3961 tcaaccttaa gaaaagtggt gaaggtcctt cgcaagagca aagagataaa agtacttttc
     4021 aagtagtatt tattggtaaa agtgaaacta tgcagatcaa aacaaaagta tccggtggtg
     4081 accccggtta tacggaaaca tcaaaaatgc tttcagaaac agcattgacc ttacttgaaa
     4141 atcttggcaa catagacaca gctccaggaa ttacaactcc aattggtgct ttaggaaata
     4201 aactaattga caagttaatc agcaaaggaa ttcgctttga agtgataagt atgtaatgag
     4261 gtgtaaatag tgagtagtga gtagtgagta gtggtaagta attagtgatt tgcaaataga
     4321 gaaaaataga tgtgttagtt gtagactaaa atattcaaaa attaaaatta aaattaacat
     4381 taaacattaa ctatgttaca tcgtccaaga aggaacagaa aatcagcagc aattcgagga
     4441 atggtgcaag aaattcatct tcaatccagt catttagttc aaccactatt tttggttgac
     4501 ggcaaaaata aacgcaatcc catcaagtca ttgccatcgc aagcacaatt gagcattgat
     4561 gtagctatca aagaaattga agcctgcatg aaattgggtg tacaaaattt tatcttgttt
     4621 cctgctgtag aaaatgcact taaagacaaa aaagcaagct atagctattc gccaaagaac
     4681 ttttatttaa aagcgattaa agaatttaaa aaagaatttc cagaatgttg tttaatcagt
     4741 gatgtagcca tggatccata cagcagcgac ggtcatgacg gctatgtaga aaacggaaaa
     4801 atcatcaatg ataagacatt gccgatttta gcgaagatgt cattagcgca agcgcaagcc
     4861 ggcatagata ttcttggtcc gagtgatatg atggatggtc gtgtgggtta tattagagaa
     4921 ctcttagatg ccgacaattt tcaggaggta ggcattatga gctattcggc aaaatatgcc
     4981 agtgcgtttt atgggccatt tcgtgatgcg ttggattctg cgcctaaaag cggagataag
     5041 aaaacctatc agatgaattt ttgcaataaa aaagaagcat tgcttgaagc tgaactagat
     5101 tataatgaag gtgctgattt tttgatggtg aagcctgcaa catgttactt agacatcata
     5161 caagatttga aacaaaattt tcctgtacct atcgctgctt atcatgtaag tggtgagtat
     5221 gccatgctga aagctgcggc acaaaacggc tggctggatt tcaataaaac actcgatgaa
     5281 acactgacag ccattcgcag agcaggaagt gatgtcataa ttacttatgg tgcaaaagag
     5341 tgggcggaag ctgttggttg attagaaata aattatgata aacatactta ttctgggtgg
     5401 tgggcggtta atcggtcaga atttattggc aaagctggca aagaataaac cttatgccat
     5461 taccactttc tccagaggga tgactaattc atctcaagat ttttcggtta accgactttt
     5521 tggcgacagg agaaaaacgg aagatctcca tcaaataaac gatagagatt gggatattgt
     5581 tattgactta tcttgctact ggccggatgc actgaagcaa caacttgaac aacaaaaagg
     5641 aaaaattgga aaatatattt ttatctctac ttcaagttta tttgattttg atgaaaataa
     5701 tccgcatttt attagtgaag atgaacctac cgttagttgt agtccggaac aaatagcaga
     5761 tgaaactggt gcttcttaca atgccaggaa agcggagtgt gagcgtgtat tacaacaatt
     5821 tccacaactc aattatgtga tgttgcgccc gggattgata ttgggaaaac atgattacac
     5881 acaacggctc tattactggc tttatcgcat cagtaagtgt caggcttatt tacaacctga
     5941 aaaattcaac aatcgcattg cattcaccga tgtagatgat ttgtgtgcaa tgattgattt
     6001 ttttattcat actcaacagc aacatactat ttatcatgcc atttcctttc atgctacgtt
     6061 agctgaaatt gtagcagtca cacaacaaaa attaaataaa aagcctgaag ttgttattgc
     6121 caccgacgaa tttctcgata agcatcaaat acatgaatgg gtggatttac cgctctggct
     6181 caaaaatgaa tttatgctga ttgataattc gagaataaaa caagccatag atttcccgct
     6241 ttcaacactt gatgcaacaa taagtaaact gcttcaatac tatgagcaag attgccattg
     6301 gccattaccg aatacttcac ctacttccct atcattagaa cgtgaacaaa tcctgataaa
     6361 tgaacttatt aacctgagtt gaaataagtg ttcattttaa aagtagttga ttttttagct
     6421 ataaccacct ccttttaatt cctcctcctt tttaagttgg aaaaaaatga gaaatcaagt
     6481 atttttttac cttcattatc ttaaccagaa ctcatgttga tgctttttta ttgcaaacta
     6541 taatatagtt cagtgagctg gttgacactg atgtcaaatg catatttttt agcttgaagt
     6601 gcatattttt ccgcggcttg ttgcagttca ttactatgtt caaaataata atctattttg
     6661 tgtgtcagct ccgtagttgt cttgaataga aagtctgaat ttaaagcaaa ctgtttggca
     6721 gcactcaaag gcgcatcggc aattatcatg ggtaagccgg cagccatggc ctccatcgtg
     6781 ctcattgatt ctacttcaac gccagcacaa tgcacatata aatctgcttc ctgatagtaa
     6841 cgcgtaatct catctttaga aacatataaa aactctactt ggttgggtaa tttgttgttt
     6901 gcaagcgttg ttaatttttc ccgtattggt ccatcaccga taatacttaa ttttatttgc
     6961 tttttatttt tacatttact aatcgcttca atcagcatat cttggcgttt ttcgacagca
     7021 tttcttccta cagttagtat atgaaaataa ggacttccct gattcctgtt ggacatgaga
     7081 aagttttctt tcatgatgcc attcgagata accacgctgt tagtcttgag tccatacctt
     7141 tttaattcgt cctgagcaaa aatacttgga catataacca catccgactt gttatatatt
     7201 tgttcgataa agaactgatt aatctttttt atcagccact tattctttat gttgatattg
     7261 taggctattt gttcagcttg tacgtgaaaa gtactgacaa ttggtttttt atattgttgt
     7321 gctatttcta tacatttcat gcttagccag aatggaaact gaacatgtac aatatcacaa
     7381 gtctctataa cagaattaat tttcttttcg tcaggaaaag caaattgtgt tttcattttt
     7441 tgcattacat ttttcacgaa tggtacaggt ggataaaatt caggcataat tattttatcc
     7501 tgactttttt tgccggtact gattactttc acttggtgtc ctttttggcg taatgctgtc
     7561 gtaaaacgtt gagtagaaat cactgctccg ttgcgggcat catcaaaaac atcaataacc
     7621 tgaacaatat tcagcgggtt catgtaataa aataagcagt taggtgctca aagataaaat
     7681 cttaatccgg aagtcgtagt ctaaaatttt cttaaataat ctcaattgta cattacatca
     7741 agaacatgtt cagtttttta gtgtattttc aacgaaattt atatacatga agaatgcgat
     7801 atttgcaatg ggattgagct tgttctattt acaagtttcg gctgttacta ttaataactc
     7861 caaagtaagc gaagtgacag tttacagaaa ttttgccaaa gaaacacggc tggctagcga
     7921 caacctcaat gaaggtgtac aagaagttat catcagcaac gtgtcaccgt ttatggatga
     7981 aaacactata cagattgcaa cgaaaggcaa tgtaaaaata ctgtcagtta gcagccggaa
     8041 taatttttta tccgacaaga aaaaaacaga cgtctcgcaa aagctgagtg attcattaaa
     8101 actgttgaat aacgaaaacg actggatagc cgagcagata gcagcttacc aaggtgagct
     8161 tgaagtttta gacaacaaca aaaaattagc caacgacaaa ttgctgttta aatcaacaga
     8221 tgtgaaagat atagctgatt tttacatcgt aaaagcatta gaaataagaa gaaaattatt
     8281 ggatttggtt catgagcaaa cggcaaacac agaaatcatt acgcgcatta cccaacaaat
     8341 aaatgacata ggcaaccagc aaaaatcagt taaagaaatc gtattgaaag taaatgcatt
     8401 gtcagctacg gctgccactt tcaagtgcag ctatgtggta agtagcgcct attggatgcc
     8461 cgtgtacgac atacgcgcaa aaaatctgac agagcctttg caagttgatt gtaaagccaa
     8521 agtagtacaa aatacaggta tggactggaa agatattaaa ttaaaactct caacagccaa
     8581 ccctgcaagc aatcaaagcc gacctgtttt atatccgctt tatgttgatt ttttccagcc
     8641 tgattattac tataaaaaaa tggaaagtaa gatttcagga gttgttgcag cacctcagat
     8701 gatgcaaaac tcaatggaca tagcacgtag cggaatggat aaggaagagt ttacagatgg
     8761 cttaagagca gatgataatg gggttacatt attggaaggc gaaaccacac aagagtacga
     8821 tatacaaact ttgcaggata tagaatcgga taatcaagcg catatagtgg gcatacagga
     8881 aataaaaatg caggccgttt ataattacca cgccgtgccg aaattagatc caacagctta
     8941 tttgctggca cgcgtgaccg attgggcaaa atataatttg ctggcgggcg aagccaatat
     9001 cttctttgac gacagttacg tgggcaaatc ctatctcaac ccgaatgtaa gtgccgatac
     9061 tttattgatt tctttaggca gagatgagaa aattaatatc aagcgcatca agctgaatga
     9121 ttatacatcg act
//
LOCUS       DAOQII010000088         9058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4089_length_9058_cov_4.695657, whole genome shotgun sequence.
ACCESSION   DAOQII010000088 DAOQII010000000
VERSION     DAOQII010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_9058_cov_4.695657"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PLF48_05725"
     CDS             complement(<1..1017)
                     /locus_tag="PLF48_05725"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNF48859.1"
                     /translation="MQNILSDIAGLQIHGNVAVNIHALMLDSRQVKKEDVFFSVKGNA
                     VDGHAFIDAVINQGARVIVCEVLPEKLHDEVVYIQTANVRETCALMACNYYNHPSRKL
                     KLVGVTGTNGKTSIATLLYQIYLGRGDKSGLLSTIVNKINEKEISSTHTTPDPIAINA
                     LLNDMVEAGCTYAFMEVSSHAVDQNRIAGLEFTGGIFTNITHDHLDYHKTFPEYLKAK
                     KKFFDNLSASAFALTNIDDKRGEVMLQNTKAKKYSYAVKSLADFRFKILENAFSGLII
                     QCDNVEVHLQMVGRFNAYNLCAIYAVCVLLGMEKQDALQEISKLKGAEGRFDTQISED
                     KIIGI"
     gene            complement(1031..3133)
                     /locus_tag="PLF48_05730"
     CDS             complement(1031..3133)
                     /locus_tag="PLF48_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNF48860.1"
                     /translation="MNVKSNIILRIYIVGAICVLFGAAVLGKIFYIQTYQNSYWKSLA
                     DSLTTEIFEIPAERGNIYSADDKLLATSIPYFELHVDFASKAMKNVIFNKNVDSLAYY
                     MWANFHEQSESAYRRDLLRARKNKNRYYLLTKKADYTLLNKIKTWPLFREGKFKGGLI
                     VEMYQERKNPYGYLAQRTIGLIRDNAPDIGLEDKYDSVLSGVQGKMLKQKIAGGEWIP
                     LKREGQIEPKNGYDIITTIDLGLQDIAETSLLNALTEYKAEFGSAILMEVKTGAIKAI
                     ANLSENSDGTYSERYNNAIGSRNEPGSVFKVAAYLALFDDGFITPSDSINTNHASAKF
                     GDVLLTDDGHNQQYTFLTPAKALAVSSNVAIAKWVTKFYNNDREKFYDKLEQFGLTEK
                     AHVEIKGEQQPLIRKPQTWSYMSLPWMAHGYEVKFTPLQILTFYNAIANNGNRMKPYL
                     VQRIVENGKVIQEFKPQTTKQKICTEQAAQWARDIMVAVVEDPNGTARSIRSFHYKIA
                     GKTGTAKMSEGTSGYTNKNLSSFVGFFPADNPLYSCIVVVGGPQGLLTTGGAVSAPIF
                     RTIADKIITSNINNEVAVNKDTLLPSYPMPSMLSDIAVIKEMNKKLKLKADIGDDWEY
                     AIIKTDSLNKKTITQVAYDKNAVPDVRGMLLDDAIALLENKGLHVGFSGKGKIISQSI
                     QAGTPIFKGSYIQLTLNP"
     gene            complement(3218..3649)
                     /locus_tag="PLF48_05735"
     CDS             complement(3218..3649)
                     /locus_tag="PLF48_05735"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNF48861.1"
                     /translation="MEEQDNIQELLQDNQADPVDVSKPAPKSDGVLFARLQRMIEMVL
                     RLLGVDDWVNYGQVMRNALFVISIVFIGVMEVFNGHLAVRMNRNINKKNEKIKELRWE
                     YMTAKTDLNQKSKQSELQKIVEPYGLKTLQEPPKKIEVKLK"
     gene            complement(3649..4587)
                     /gene="rsmH"
                     /locus_tag="PLF48_05740"
     CDS             complement(3649..4587)
                     /gene="rsmH"
                     /locus_tag="PLF48_05740"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNF48862.1"
                     /translation="MRFGQELITPNHNAMYHVPVMLQECIEGLRIIPDGIYVDVTFGG
                     GGHSKAILNELGTNGKLFAFDQDEDVIVNVPQDKRFTLIQANFRHITRFLKLYGITQV
                     NGILADFGISSHQIDEASRGFSTRFNAELDMRMNRKEGISAKEIINTYSAGQLQKMLS
                     EYGEVQNAKTLAMAIVEYRAAQHIETTDELKAICSSVSKGKEVKYLSQVFQAIRMEVN
                     DEVNALKDFLKQAKALLVPGGRLVVMSYHSLEDRPVKNYMKMGVFEGEPDKDMFGNYM
                     HDLIVITKKPTEATAEEVKQNPRARSAKLRIAEKNK"
     gene            complement(4550..5011)
                     /locus_tag="PLF48_05745"
     CDS             complement(4550..5011)
                     /locus_tag="PLF48_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48863.1"
                     /translation="MIGLTGNYEVKVDSKGRVRLPANLLQQLPEDMRNGGFVMNKGMM
                     NCLRLYPKKQWDAVTLEMNRLSYFRKDESNFLRFFYQWASTVEKDTNERILIPKRLLE
                     KVGIKEDVTITAWHDVIEIWDTATYNASIVEPENFADMADEVWSRINNAKS"
     gene            complement(5323..6201)
                     /locus_tag="PLF48_05750"
     CDS             complement(5323..6201)
                     /locus_tag="PLF48_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48864.1"
                     /translation="MEKIEYITKRSITGPLVILVFALIFGGMFIFLAAEATYFELGFP
                     LVFGVIVIVFSVLFFLEGFYKHKLLFSTNGVAYLDYKNNLLKAIHNSEIKAINYHKTT
                     GKSTIHTVEFETDHEYYTFNSEYVKNWKEILDYLYTLKIPDKTNNSYYGKSKRTLTSP
                     QSYMFLFVGIALLLFALIYAVVVKYNIDKQNLVKRDYFFTLSSQPEYHKQKHGHYYTI
                     ETQQAAGFSMNVGSKHDEVVTSYNANDTIILQISERDYKVKICKEIQPNFWDKHYDYN
                     TIIIYAICKYGHEYKL"
     gene            complement(6295..7698)
                     /locus_tag="PLF48_05755"
     CDS             complement(6295..7698)
                     /locus_tag="PLF48_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNF48865.1"
                     /translation="MQLLHAEIGDKSGIYDAAGRYMILRGVNYNCLGYYWQANSQVAV
                     QKQPEEADFKLMATYGFNCVRLLFSWSKLEPAQGQYDYNYIEQIKKYIEIAGKYGLYV
                     LIDMHQDAWGKYIATPQSVNCSSPNKGWDGAPLWATLTDSASTCKNTTDRESAPAVVH
                     AFENFWNNKQNIQTACLNAWAELAKNCCVYENVLGYDLLNEPGLGYSNLLDGSSKMSR
                     FYGDAIRRIRAAEQSVNGFQHIIFFETSITQNGNPLPFIPSFGFTNDKNIAFAPHTYF
                     EAISYALTIEQGYDLLKTISALFKTPMLIGEYGFFDGYATDSSKLARFAVKEDANFGS
                     STYWQWSQAPGDPHSISYDGLLFSQTNMALIELDKNGNFTGNINKDYLKVLSRSRPVA
                     VAGKPTYLSSNINSGKLSLYASLSGSGTTILWVPSGHGTPVVSGTNIVKNEIQQVDGG
                     FRIAVEVNGDYKIHLSF"
     gene            7916..8257
                     /locus_tag="PLF48_05760"
     CDS             7916..8257
                     /locus_tag="PLF48_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48866.1"
                     /translation="MKIRFLYLVSSVLFTMIACKKNSSDYVPTCGSTTPSFASSVNPL
                     IQARCATSGCHNSTTKADGVDLSTYALIVTHKEHIKSEVASGGMPEGSSLTSAQKDSM
                     LCWINAGAPNN"
     gene            complement(8328..>9058)
                     /locus_tag="PLF48_05765"
     CDS             complement(8328..>9058)
                     /locus_tag="PLF48_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48867.1"
                     /translation="VPKKSEPAKPEPKLKKQDEKLKTPVKTEKEIKPPLKSTKIEAEK
                     NNSKTDSTSKTTPAKSSDKKQPEKKGKTTDVPPAKFVMPPPQPGVKRKVIVDYKNVPD
                     EVLRALYEKYPHGYNKAIIRFMNAKKEMVSAVPIELEDVSYLVKVSTQLQKMVDDYDE
                     DEMFTEVNVPAEKEIPAGAGGDFDEFDRSDDDDAPKSKSKGRVRSADDLEYGFDDEEE
                     EDDDDDDDDDDNESDDVTDDDDDE"
BASE COUNT         2639 a         1813 c         1454 g         3152 t
ORIGIN      
        1 aatgccaatt attttatctt cagaaatctg cgtatcaaaa cgcccttcag ctcctttcag
       61 cttggaaatc tcctgtaaag catcctgttt ttccatacct aacaatacac ataccgcata
      121 aatagcacat aaattatagg cattgaatct gcctaccatt tgcaaatgca cttcaacatt
      181 atcacattgt atgattaatc ctgaaaatgc attttccaaa attttgaaac gaaaatcagc
      241 taaggatttt acagcatacg aatatttttt tgccttagta ttttgcagca ttacttcccc
      301 ccgtttatca tcaatattgg ttagtgcaaa agcagaagca gagaggttat caaaaaattt
      361 ctttttggct tttaagtact ccggaaatgt tttgtgataa tctaaatgat catgtgtgat
      421 attggtaaat atgccaccag taaattccaa tcctgctatt cgattttgat caacggcatg
      481 tgagcttact tccatgaagg catatgtaca accggcttca accatatcat tcaacagtgc
      541 attaattgct attggatccg gggttgtatg tgtggatgaa atttcttttt cgtttatttt
      601 gtttacgata gtagaaagca acccgctttt atctccacgg cctaaataaa tctgataaag
      661 caaagtagct atggatgttt taccattcgt tccggttacg cctactaatt ttaattttct
      721 ggaaggatga ttgtaataat tacaagccat taaggcgcaa gtttctctta cgtttgccgt
      781 ttgaatatag accacttcat cgtggagttt ttcaggcaat acttcacata cgattacgcg
      841 agcaccttga ttaattaccg catcaataaa agcgtgacca tctacagcat ttcctttcac
      901 ggaaaaaaaa acatcctctt tcttcacttg acgagaatct aacatcagtg catgtatatt
      961 cactgctaca tttccatgta tttgcagacc agctatatca gataatatgt tttgcaatga
     1021 tttcaatttg tcagggattt agtgtgagtt gaatgtatga gcctttaaaa attggcgtac
     1081 ctgcttgtat agactggctt attatttttc ccttacctga gaatccaaca tgcaatcctt
     1141 tgttttccaa caaagcaatc gcatcatcaa gcaacatacc tcttacatca ggtactgcgt
     1201 ttttatcgta agcaacctga gtaatcgttt tcttattcaa gctgtctgtt tttattattg
     1261 catattccca atcatcgcct atatctgcct ttaattttag ctttttattc atttctttta
     1321 tgacagcaat atctgacagc atacttggca tcggatagga aggaagcagg gtgtctttat
     1381 ttacagctac ttcattatta atatttgaag taattatttt atcagcaatc gttctaaaga
     1441 tgggcgcaga aaccgcacct cctgtagtca gtaaaccttg cggcccaccc actacaacaa
     1501 tacaagagta cagtggatta tcagctggaa agaaaccgac aaaggaagac agatttttat
     1561 tggtataacc acttgttcct tcgctcattt tcgcagttcc tgtcttacct gcaattttat
     1621 aatgaaatga acgaatggat cgagcagttc cgttaggatc ttccaccaca gctaccataa
     1681 tatctcttgc ccattgtgca gcttgttctg tacaaatttt ttgcttggtt gtttgtggtt
     1741 taaattcctg aataactttt ccattttcaa ctatacgttg caccaaatat ggtttcattc
     1801 tattgccatt attggcgata gcattataaa atgtaaggat ttgaagtggc gtaaatttta
     1861 cttcataacc gtgtgccatc caaggcaaag acatatagct ccatgtttga ggttttctaa
     1921 tcagaggttg ctgctcacct tttatttcaa catgagcctt ttctgtaagt ccgaattgtt
     1981 ctaatttatc ataaaatttt tcacgatcat tattataaaa ctttgtaacc cattttgcga
     2041 tagctacatt agacgaaacc gccaaagctt tagccggagt taagaaagta tattgttgat
     2101 tatgcccgtc atccgttaat aatacatcac cgaattttgc actggcatga ttagtattga
     2161 tggaatctga tggtgtgata aaaccatcat caaacaaagc cagataagca gccactttaa
     2221 aaacagaacc gggttcattg cgtgaaccga ttgcattatt gtagcgctcg gagtaagtgc
     2281 catccgaatt ttcacttaaa ttggcaatgg cttttatcgc tccggttttc acttccatca
     2341 gtatagcaga gccaaattca gctttgtact cggtaagggc attgagtaaa gaagtttctg
     2401 caatatcctg caaacctaaa tcgatagtcg ttataatatc atagccgttc tttggctcaa
     2461 tttgtccttc tcgttttaaa ggaatccatt cgccaccggc tattttttgt ttcaacatct
     2521 tgccttgaac gcctgataaa acagaatcgt atttgtcttc taaaccgata tcaggagcgt
     2581 tatcacgaat caaacctatc gttctttgtg ccagataacc ataaggattt ttgcgttcct
     2641 gatacatctc aacaatcaat ccgcctttaa attttccttc tctgaatagc ggccatgttt
     2701 tgattttgtt gagtaaggta taatctgcct ttttagtcaa caagtaatat cggtttttat
     2761 tttttcgagc tcttaatagg tctcttcgat atgcgctttc tgattgctcg tgaaaattag
     2821 cccacatata gtaagccaaa gaatcgacgt ttttattaaa aattacattt ttcattgctt
     2881 tagatgcaaa atctacatgc aattcgaaat agggaattga agtagctaat agtttatcat
     2941 cagcagaata aatgtttccg cgttcagctg ggatttcaaa aatttctgta gtcaaactat
     3001 cggccaatga tttccaataa ctgttctgat aggtttgtat ataaaagatt ttacctaata
     3061 cagcagcgcc aaacaacaca cagattgctc ctacaatata aattcgcaat atgatatttg
     3121 acttgacgtt cattcgtgtt tgttggtgat tgatgattag ttattggtga ttaatttttg
     3181 gatctccaca cttactactt tttacttacc tctctattta tttcagcttt acttctatct
     3241 tcttcggtgg ttcttgtaat gttttaagtc cgtacggttc tactattttt tgcaattcac
     3301 tttgtttgct tttttggttt aaatctgttt tagcagtcat gtattcccat cgcaattctt
     3361 ttattttctc attcttcttg ttgatatttc tgttcatcct cactgctaag tgaccattga
     3421 agacctccat cactccgata aatactatac tgataacaaa taatgcatta cgcatcactt
     3481 gcccataatt tacccaatca tccaccccca gtaagcgcaa taccatttct atcatacgct
     3541 gtaatcgggc aaacaatact ccatccgact taggtgctgg ttttgacaca tcaaccggat
     3601 cagcttggtt atcctgtagc aattcttgta tattatcttg ttcttccact atttattttt
     3661 ttctgcaatt cttagtttag cacttctggc cctcggattt tgttttactt cctctgctgt
     3721 cgcttctgtt ggttttttag taatgactat caaatcatgc atgtaattgc caaacatatc
     3781 tttatccggt tcgccttcga atactcccat tttcatataa tttttcaccg gtctgtcttc
     3841 taatgaatga taactcatta caaccagtct gcctccggga actaacaagg cttttgcttg
     3901 ttttaaaaaa tctttcaatg cattcacttc gtcattcact tccattctaa tagcctgaaa
     3961 tacctgtgat aaatacttta cctccttccc ttttgaaacc gaactgcaaa ttgcttttaa
     4021 ctcgtcggtt gtttcaatgt gttgcgctgc cctgtattcc acgattgcca ttgccagcgt
     4081 ttttgcattc tgtacttcgc catattcact cagcattttt tgaagttgcc cggctgaata
     4141 agtgttaata atttcttttg cactgatgcc ttcttttctg ttcattcgca tatccaattc
     4201 tgcattgaag cgagtggaaa atccgcgtga agcttcatca atctgatgtg acgagatgcc
     4261 gaagtcagcc agaattccat ttacttgtgt gatgccgtat aatttcaaaa aacgtgtaat
     4321 atgtctgaag tttgcctgta tcagtgtaaa tcgtttatct tgcggaacat tcactattac
     4381 atcttcatcc tgatcaaatg caaacaattt cccattagtg cccaactcat ttaaaattgc
     4441 ttttgaatga ccacctccac caaacgtaac atctacataa attccatccg gaataatccg
     4501 caaaccctcg atacattctt gcaacataac cggaacgtga tacatggcat tatgatttgg
     4561 cgttattaat tcttgaccaa acctcatcag ccatatctgc aaaattttcc ggctctacta
     4621 tcgatgcgtt gtatgtggcc gtatcccaga tttcaatcac atcatgccaa gcagttatgg
     4681 ttacatcttc tttaattcct actttttcca ataatctttt tggtatcaga atccgttcgt
     4741 tggtatcttt ttcgactgtt gatgcccatt gataaaaaaa gcgcaggaag ttgctttcat
     4801 cttttctgaa atagcttaag cggttcattt ccagtgtaac ggcatcccat tgttttttgg
     4861 ggtatagccg caaacaattc atcatgccct tattcatgac aaagccaccg ttgcgcatgt
     4921 cttccggaag ttgctgcaaa agattggcag gcaaacgcac tctgcccttg ctgtcaactt
     4981 ttacttcgta atttccggtt aatccaatca taagatttgt ggtatcacgt acaaaaatag
     5041 aagatatttg acaaaatctc ccacttctta ccacaatttg tcccaaatac caatgtgaat
     5101 atctcaaatt atgttaataa ctctgtagat atctatgatg tagatgtgca aaatactttt
     5161 gaagtataat agaataaggg tttgtttata aacagatgtt tatgaacaca gtttgaaaaa
     5221 gaggataaaa attctttttc atcgagataa ggtgaataaa aagttgaata atatctcagc
     5281 taaatgaagc agttgatcta aattgtaaat aatgggaaac aattatagtt tgtattcatg
     5341 cccatattta catatagcat aaattataat tgtattgtaa tcataatgct tatcccagaa
     5401 attgggttga atttccttac atatttttac tttataatct cgttcagaga tttgtaaaat
     5461 tattgtgtca ttagcattat aacttgttac aacttcatca tgtttagagc ccacattcat
     5521 agaaaagcca gctgcttgtt gagtttctat agtataataa tgcccatgtt tttgtttatg
     5581 atattccggt tgggaagata gggtaaaaaa ataatctcgt ttaacaagat tttgtttatc
     5641 aatattatac ttaactacta cggcatatat gagggcaaat aagagcaatg caataccgac
     5701 aaataaaaac atataacttt gaggagaagt aagtgttctt ttcgatttac cataatagga
     5761 attattggtt ttgtcaggta tttttaaggt atataaataa tccagaatct ctttccaatt
     5821 ctttacatat tcgctattaa aagtataata ttcatggtct gtttcaaatt cgacagtatg
     5881 tattgtactt ttgcctgtag tcttgtgata atttatagct ttaatttcgc tgttgtgtat
     5941 ggcttttaag agattgttct tataatctaa ataagctact ccatttgtag aaaataataa
     6001 tttatgctta tagaaacctt ccagaaagaa aagaactgaa aaaacaatta caattacacc
     6061 aaatactaat ggaaaaccta attcaaaata tgttgcttca gcagcaagaa aaataaacat
     6121 tccaccaaaa ataagtgcaa atactaaaat aactaatggc cctgtaatac ttcgttttgt
     6181 aatatattct atcttttcca tgttgtacta acaacaatct tcaaaaaaag attcccgata
     6241 gatcaaattt attaaaatgt aatttttaaa tttaacggca gaatcgaaag gtacttaaaa
     6301 gctcaggtga attttataat ctccgttaac ttccacagca atccggaaac caccatctac
     6361 ttgctgtatt tcgttcttta caatatttgt acccgacaca acgggtgttc catgtcctga
     6421 tggcacccaa agtatcgttg tgccggaacc actcaatgag gcgtataaac ttaatttacc
     6481 actattaata tttgatgata aataagtagg ttttcctgca acagctacag gtctgcttct
     6541 gcttaagact ttcaaataat ctttgttgat atttccggta aaatttccat ttttatccaa
     6601 ttcaattaat gccatattcg tttgagaaaa aagaaggcca tcgtagctga tactatgcgg
     6661 atcgccgggt gcttgtgacc actgccagta ggtagagctt ccgaaattgg catcttcttt
     6721 tactgcaaaa cgagccagtt ttgaactgtc agtcgcatat ccgtcaaaaa agccatactc
     6781 accaatcagc atgggtgttt taaacaatgc agaaatggtt ttcagtaaat cataaccttg
     6841 ctcaatggta agtgcatatg atatagcttc aaaatacgtg tgcggggcaa aggcaatgtt
     6901 tttatcgttt gtaaaaccaa acgacggaat aaatggaagc gggttcccat tttgcgtgat
     6961 actggtttca aaaaagatga tatgctgaaa gccgtttaca gattgctctg ccgcacggat
     7021 gcgacgtatg gcatctccat aaaatctact catttttgaa gaaccatcta aaagattgct
     7081 ataacccagt cccggttcgt ttaataaatc ataacccaac acattttcgt atacgcagca
     7141 atttttagct aactccgccc atgcattcag gcatgcagtt tgaatatttt gtttgttgtt
     7201 ccaaaaattc tcaaaagcat gcactacggc aggtgcactc tctctgtctg tcgtattttt
     7261 acaggttgaa gcactatcag taagcgtagc ccagagtggt gcaccgtccc aacctttatt
     7321 agggctggaa caattgacag attgtggtgt ggcaatatat tttccccagg catcctggtg
     7381 catatctatc aatacataca agccatattt gccggctatt tcaatatatt tttttatttg
     7441 ttcaatataa ttatagtcat actgcccttg agcgggttct aatttgctcc aactgaatag
     7501 caaacgcaca caattaaatc cataagtagc cattagttta aaatctgcct cttcgggctg
     7561 tttttgtaca gctacttgcg aatttgcctg ccagtaatat cccagacaat tataattaac
     7621 tccgcgcaat atcatatatc tgcctgctgc atcatagatt ccggacttat ccccaatttc
     7681 agcatgcaaa agctgcaagc cttgttgatt gctattttcc tttttacaac taattagaac
     7741 aaagcatacc aaaagaagta agatagagta tcttattttc tgcatcacat tatgaataat
     7801 ccttaaatct aactgatttt taacaatttt atgcgatatt tttttaaaaa atctatgaat
     7861 ctataaaaat atccgtttat tttcaccgtt atattaaaca ccaaaacaat tttttatgaa
     7921 aatcagattc ctttatctcg tttcctctgt gctatttacg atgattgcct gtaaaaagaa
     7981 cagttcagat tatgtaccaa cctgtggcag tacaacacct tcatttgctt caagtgtgaa
     8041 tccattaata caagcaagat gtgctacatc aggctgtcac aatagcacta ctaaagctga
     8101 tggtgtagat ttaagcactt atgctcttat agtaacacat aaggagcata tcaagtctga
     8161 agttgcaagt ggcggaatgc ccgagggaag ttctttaact agtgctcaaa aagattctat
     8221 gctttgctgg ataaatgcag gagctccaaa taattaatgt aatatctttg taagtaaaaa
     8281 agaggttgct caagtgcaac ctcttttttt ataatcgtac tgatagacta ttcatcgtca
     8341 tcatcatcag taacatcatc gctttcatta tcatcatcgt catcgtcgtc gtcatcatct
     8401 tcctcctctt catcatcaaa accatattcc aaatcatcag cacttcttac tctgccttta
     8461 gattttgatt taggagcatc atcgtcatca cttctgtcga attcatcaaa gtctccgcct
     8521 gcacctgcag gaatttcttt ttcagcagga acatttactt ctgtaaacat ttcatcctca
     8581 tcataatcgt ctaccatttt ttgcaattgc gtagaaactt ttaccaaata agaaacgtct
     8641 tccagctcga taggcacagc agataccatt tcttttttag cattcataaa acgtataatt
     8701 gccttattat acccatgcgg atatttttca tataatgccc tcaagacttc atccggtaca
     8761 tttttataat caactattac ttttcttttt acaccgggtt gtggtggtgg cattacaaat
     8821 ttagccggag gtacatcagt tgttttacct tttttttcag gttgtttttt atctgaagat
     8881 ttagctggcg ttgttttact cgtactatca gttttggagt tatttttttc agcttctatt
     8941 ttagtcgatt tcagcggtgg tttaatttct ttttcagttt tcaccggtgt tttcagcttt
     9001 tcatcttgct ttttcaattt cggttctggc ttggcgggtt cagatttttt aggcactg
//
LOCUS       DAOQII010000089         9031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4102_length_9031_cov_5.507353, whole genome shotgun sequence.
ACCESSION   DAOQII010000089 DAOQII010000000
VERSION     DAOQII010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_9031_cov_5.507353"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="PLF48_05770"
     CDS             complement(<1..1654)
                     /locus_tag="PLF48_05770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF48868.1"
                     /translation="MKLTFSILILMLNMLIMPVIAQNQSYKSIPFAKPMDLPFDLAGC
                     FAEPRPSHFHSGIDIKTNEKEGYPVYAIYDGYISRIKVSAFGYGKALYITHTNGFTSV
                     YGHLSRFTDEIEKYVHDEIYKQQLSEIDIYPASNKLPVKKEQQVAYSGNTGGSSGPHL
                     HFEIRDTKTEHALNPILFNYAYKDNQAPTISELKIYNINESYYSAETNAIKLKKIDDY
                     LSPSGPVVATANTIGFSLKGYDAQENSTNKNGIYRIELYANNILQYAFTQDEIDFSTT
                     RGVAAFVDFKEQCTSSLDNYLCFKLPNNPLKIFDNLSTTGILRVKDKDTLAILIRCFD
                     VNKNETQVRFKLTVNLTPKPSKKNAFNYAKKNLLQKPKCHVSFPENTFYDHLTLSYAM
                     DTAKKSGIYSSIHKIGNNQFIPLYKSSEIEIMSRKLPVADRNKACIVVRNHKGKEKFL
                     TTETHANWLTAQTKELGDFYIKTDKEKPQISLLNARQDSFKDNRILVKITDTRSGIKS
                     YVGYIDGNWINFYYDAKEDTIEYQMDEFCKSGTHELKIIVTDQ"
     gene            1796..3232
                     /locus_tag="PLF48_05775"
     CDS             1796..3232
                     /locus_tag="PLF48_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNF48869.1"
                     /translation="MLFDKYQTKENFWDEMAFADGRIRPHYQNLFDELNNMDISVLNQ
                     KEEIAKKMFMNQGITFTVYSGDEGIERIFPFDIIPRIITANEWDFISRGIEQRMKALN
                     LFIHDIYHEQNILKEGIAPVDLIATCPHYNREVFGIDVPHNIYVHIAGIDLVRDADGT
                     YYVLEDNLRTPSGVSYMMENRDVTKSIFPELMNRYNVRRVSDYPLELQKILVTLAPNQ
                     MQNPCVVILTPGAYNSAYYEHTFLTRYMGVELVEGRDLVVENHTVYMKTTHGLKQVDV
                     IYRRVDDEFLDPLVFRPDSLLGVPGLLSAYRKGRVSIVNAMGNGVADDKAVYAFVPDM
                     IKYYLNEEPLLKNVPTYKMENLDERKYVFENMHRMVVKRTNESGGYGMLMGESASDEE
                     IEKFKRAIESEPRFYIAQPTIKISTVPCYMQGKLTPRHVDLRPYALYGPDGFKLVSGG
                     LTRVALKEGSLVVNSSQGGGSKDTWVID"
     gene            3268..4206
                     /locus_tag="PLF48_05780"
     CDS             3268..4206
                     /locus_tag="PLF48_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNF48870.1"
                     /translation="MLSRIADSLFWMNRYVERAEGMLRMLKINYATSLDHSGSHAYDW
                     QPVLSIFTYLEDDKINSMRHQSQEVLKYIISDKQNENSIRSIITKARENARGLQDQIT
                     KEVWESINEYYLRFSSGRIDELLDTNQQAYAISSLIDQSLFYYGVAEVTMPRGEGWNF
                     MGIGKNIERALQTADILEIRFQDINYDLSNPMDILYWKNLLLSVSGYEFYLKTYPTGL
                     QTQNVLNMILMNTDFPRSILYSVKKIYFIMEKLAHENTDDEKTLLRKIGKLKSQIEYA
                     DLQSIQQIGLHNFLEELREELYQFSSELSKIYFAYR"
     gene            4227..5165
                     /locus_tag="PLF48_05785"
     CDS             4227..5165
                     /locus_tag="PLF48_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF48871.1"
                     /translation="MSIYKIHHITKYLYDREVIENANQIKIFPFPKEGQDILSQEIKI
                     SGNPDIFRFFDYWGNATGYFTQNPPHHELVIDSRLIVRTIGQEIDESRSSMQEWVEIH
                     EQIKSDIILFDYALKEKIENQELIDDLIEQLALRKQPPLQAALIINKYIYENYKYVKG
                     ITTIETTIDEIIQLKSGVCQDFAHLLLHILRTLQIPSRYVSGYICPNKSGMRGEGATH
                     AWVDVYLPNFGWIGIDPTNNCYANVNHIQLAVGRDFADCTPIKGTFKGPANQDLLVYV
                     SVGYEDGTVFEDTNTVLMQKEQTRIAPALLIQQHQQ"
     gene            complement(5226..6989)
                     /locus_tag="PLF48_05790"
     CDS             complement(5226..6989)
                     /locus_tag="PLF48_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNF48872.1"
                     /translation="MEVRRLFDFLHYQKENHPQKIAIASMKFDKWWECSTDELIEKSD
                     KFSLGLLKLGINVGDKIAVITSANRTEWNICDQGIGQIGAINVPLYPTISESDYEHIL
                     KHSESKLCIVSDEKLFEKVKNLKNRVPSLQEIYTFDNVPGAKNYEEIMTLGHDDDVSK
                     IKAISDTITPDSLATLIYTSGTTGVPKGVMLSHNNIVSNVLTVGAELPIEPGQSALSF
                     LPLCHIFERMVIYTYLYAGINVYYSDIDTLSDKLGFVKPHFFTTVPRLLEKVYEKVMT
                     GGLQSTGIKKKLFLWAMDLADKYDYDQKPSFMFTIADKLVFSKIRERLGGRVKGIVTG
                     SAACPLKIARVFSAMGIPIREGYGLTETSPVITFNRFSEGGAMLGTVGMPIKDVEVKI
                     AEDGEILAKGPNIMMGYYKELEKTREVLTEDGWFSTGDVGTFVTNYAGNKFLKITDRK
                     KELLKTSGGKYVAPAPIESKFRENFFVEQIMVVGESKKFVSALIVPAYAQLEQFAKEN
                     TITYSTKDELINNPKVIAKYQSIVDELNPNFSHIEQIKKFKLLANEWTPDTGELTPTM
                     KLKRKIINDKYAKEIDAIYND"
     gene            complement(6979..8763)
                     /locus_tag="PLF48_05795"
     CDS             complement(6979..8763)
                     /locus_tag="PLF48_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNF48873.1"
                     /translation="MSYTRIFDIIDYQQQWYPLDTCFARREDKKNWIRYSTHDFIHLA
                     NQLSAGLLKLGLQKGDKVAIISVSNRPEWHFVDLACQQIGVIDVPIYPTISAKEYEYI
                     FTHAEIKYVFLSDKLMHRKIREVLPKIPTLKGVFAFEEIEGVEHWKNLLSDESLYIRQ
                     ITEIKNSIQPTDLATIIYTSGTTGTPKGVMLSHQNIVSNVQACLKAIPLEAKCVVLSF
                     LPLCHVFERTINYVYLAAGAQIYYADGLETISEHLQDIKPHYFTTVPRLLERVFEKII
                     KKGNDLKGIKKQLFFWSVKQAESAELGEPKSFFLESNLNVANKLVFEKWRQALGGRIR
                     AIICGSAPLQKKYANIFTNAGIPVLEGYGLTECSPVVSVVPFKRKCFRAGCVGRVLEN
                     VEVKLADDGEILVKGPNVMLGYYKNEEETKKAFDKDGWLLTGDIGEFTSDQNLKITDR
                     KKELFKTSGGKYVAPSPIENKLKESFFIENVSLIGDGEKFISALVLPNFEELNIWAKK
                     EKLAFNSPEDLIQLTETKQLYKEIITELNVNFGKVEQVKHFELLADEWSVDNGLLTAT
                     MKLRRKRIKEKYSDLIRQIYNKNTPYGS"
BASE COUNT         2699 a         1732 c         1716 g         2884 t
ORIGIN      
        1 cttggtcggt aacaataatt ttcaattcat gtgtgccgct tttacaaaat tcatccatct
       61 ggtattcaat agtgtcttct ttggcatcgt aataaaaatt tatccagttg ccgtcaatat
      121 agcccacata agattttatt cccgatcgcg tgtctgtgat tttgaccaga atgcgattat
      181 ccttgaatga atcttgtctt gcattgagca ggctgatttg aggtttctct ttatctgttt
      241 taatataaaa atcaccgagt tctttagttt gtgcagtcag ccagttggca tgggtttctg
      301 ttgtcaaaaa cttttctttt cccttgtgat tgcgcactac aatacaagct ttgtttctgt
      361 ctgctacagg aagttttcga ctcattattt ctatctcact tgatttgtat aatggaataa
      421 actgattgtt acctatttta tgaatactcg agtaaatgcc cgatttttta gcagtatcca
      481 tggcataaga taaagtcaga tggtcgtaaa atgtattttc gggaaaagaa acatgacatt
      541 tcggtttttg caacaggttc tttttagcat agttaaatgc atttttttta ctcggctttg
      601 gcgttaagtt aacagttaat ttaaatcgaa cttgcgtttc atttttattg acatcaaaac
      661 atcggatcaa aatggcaagc gtgtctttat ctttcacccg caggatgccg gtagtactga
      721 ggttgtcaaa tatctttaac ggattgttgg gcaatttaaa acacaaataa ttgtccaaac
      781 ttgacgtaca ttgttcttta aaatctacaa aagccgccac ccctcgtgta gtagaaaaat
      841 caatttcatc ttgagtaaaa gcatattgaa ggatattgtt tgcatataat tcaatacggt
      901 atatcccgtt tttgttagta gaattttctt gcgcatcata tccttttaaa gaaaatccta
      961 ttgtgttggc agttgccact accggaccag aagggcttag ataatcatct atctttttta
     1021 atttaatggc atttgtttct gcgctgtaat agctttcgtt gatgttgtaa atctttaatt
     1081 ctgaaatagt gggtgcttga ttgtctttgt atgcataatt aaacaaaatc ggattaagcg
     1141 catgttctgt ttttgtatca cgaatttcaa aatgcaaatg cggaccgctg ctgccgcctg
     1201 tatttccgga gtaggctact tgttgttctt tttttacagg aagcttattt gaagcagggt
     1261 aaatatcaat ttcggataat tgctgtttat aaatttcatc gtgtacatac ttttcaattt
     1321 catctgtaaa ccgactgaga tgcccataaa cgctggtaaa tccgtttgta tgggtgatat
     1381 aaagcgcctt gccgtagcca aatgccgata cttttatccg ggatatgtag ccatcataaa
     1441 tagcataaac aggatagcct tctttctcgt tggttttaat atcaataccg gaatggaaat
     1501 gtgacggacg aggctcggca aaacatcctg ctaaatcaaa tggcaaatcc atgggcttgg
     1561 caaacgggat agatttatag gattggtttt gagcaataac aggcataata agcatattca
     1621 gcatcagtat caaaatagag aaagttaatt tcatacaact aaattacaag aaattgtcgt
     1681 tcacacacta tgtgatataa ggtgtgatat ttctttaata ttgctcaatc ttcatcattc
     1741 gttttatctc atttttattt ccaaaaaaaa tgtagtaagt tgaggaagtt aatttatgtt
     1801 gttcgacaaa tatcaaacta aagaaaattt ctgggacgaa atggcctttg cagacggtcg
     1861 tattcgtccg cattatcaga atctgtttga cgagctgaat aatatggata tttccgtatt
     1921 gaaccagaaa gaggaaattg caaaaaaaat gttcatgaat caaggaataa cttttacggt
     1981 ctattccggt gatgaaggca ttgaacgcat tttcccgttt gatatcatac cccgaattat
     2041 tacagctaac gaatgggatt ttatctccag aggcatcgag caacggatga aagcactcaa
     2101 cttgtttatc catgatattt accacgaaca aaacatcctc aaagagggca ttgctccggt
     2161 agatttgata gctacttgcc cgcattataa ccgggaagta tttggcatag atgtaccgca
     2221 taatatttat gtacatattg caggcatcga tttagtgcgc gatgctgatg gcacgtatta
     2281 tgtactggaa gacaatctgc gcacgccctc cggtgtatcc tatatgatgg agaaccgaga
     2341 tgtaaccaaa tcaatttttc cagagctgat gaaccgctac aacgtaagac gcgtcagcga
     2401 ttatccttta gaactgcaaa aaatattagt tacgctcgca cccaatcaga tgcaaaatcc
     2461 ttgtgtggtt attctcacgc cgggtgctta taattctgcc tactacgaac atacttttct
     2521 aacacgatat atgggcgtgg agctggtgga gggccgggat ttggtggtag aaaatcatac
     2581 agtgtacatg aaaaccacgc atggcttaaa acaagtggat gtcatttatc gcagggtaga
     2641 tgatgagttt ttagatcctt tagtatttcg tcctgacagt ctcttgggtg tacccggact
     2701 tctttctgct tatcgcaagg gcagagtaag cattgtaaat gccatgggta atggcgtagc
     2761 cgatgataaa gccgtgtatg cctttgttcc tgatatgata aaatattatt tgaatgaaga
     2821 gcctttacta aaaaacgtac ctacttataa gatggaaaat ttggatgaac gcaaatacgt
     2881 ttttgaaaac atgcacagaa tggtggtaaa gcggacaaat gaaagtggcg gttatggtat
     2941 gctgatgggt gaaagtgcaa gtgatgaaga aatagaaaaa tttaaacgtg ctatcgagag
     3001 tgaaccgcgt ttttatattg cacagcccac gattaaaata tcaaccgtgc cttgttacat
     3061 gcaaggaaaa ctaactcctc gccatgttga cttgaggcct tatgccttat atggtccgga
     3121 tggttttaag ttggtttcag gcggcctgac tcgcgtggca ttaaaagaag gttctttggt
     3181 ggtcaactca tcacaaggtg gtggcagcaa agacacatgg gtgattgact gaaagaaatg
     3241 agatttatat tacatattga gtagtgtatg ttgagtagaa ttgcagattc gttattttgg
     3301 atgaaccgct atgtagagcg ggctgaaggc atgttgcgca tgctgaaaat taattatgcc
     3361 acttccttag accatagcgg aagccatgct tatgactggc aacctgtttt gtccatcttt
     3421 acttatctgg aggatgataa aatcaacagc atgcgccatc agtcgcaaga ggtattaaaa
     3481 tatatcattt ctgacaaaca aaatgaaaat tctattcgct ccattattac aaaggccaga
     3541 gagaatgcac gcggattaca agaccaaatc actaaagaag tttgggaaag catcaacgaa
     3601 tattatcttc ggtttagctc cggcagaata gatgaattgc tcgatactaa tcagcaggct
     3661 tatgctatca gctcgcttat agaccagagt ttattttatt acggagtagc tgaagtaacg
     3721 atgccaagag gcgaggggtg gaattttatg ggcatcggaa aaaatatcga aagagcatta
     3781 caaaccgcag atattctgga aatacgtttt caggatatca attatgacct cagtaacccg
     3841 atggatattc tctactggaa aaatttattg ctttcggtat ccggatatga gttttatctg
     3901 aaaacatatc ctaccggttt gcaaacacaa aatgtgctga acatgatttt gatgaatacc
     3961 gattttccaa ggtctatctt atattctgtg aaaaagattt atttcattat ggaaaaactg
     4021 gcacatgaaa atacagatga tgaaaaaaca ttgctgagaa aaatcggcaa gctgaaaagt
     4081 cagattgaat atgccgatct gcaatctatc cagcaaatag gattgcataa ttttcttgaa
     4141 gaattgcgcg aagaacttta tcagtttagc agtgagctca gtaaaatcta ttttgcctat
     4201 cgttaatccc attctttatc tcatttatgt ccatttacaa gattcaccat attaccaaat
     4261 atctatacga ccgtgaggtg attgaaaatg ccaatcagat aaaaatattt ccattcccta
     4321 aagaaggaca ggacatattg agtcaagaaa ttaagataag cggcaatccg gatatcttta
     4381 ggttttttga ttactggggt aatgcaaccg ggtatttcac tcagaatcct ccgcatcatg
     4441 aattggtcat agacagccgc ctgattgtac gaaccattgg tcaggaaatt gatgagagcc
     4501 gttcatccat gcaagaatgg gtcgagattc atgaacaaat caagagcgat attatcttgt
     4561 ttgattatgc cctgaaagag aaaattgaaa accaggagct tattgatgat ttaattgaac
     4621 aactggcact tcgcaaacaa ccgccccttc aagctgcgtt aataataaac aaatatattt
     4681 atgagaacta taaatatgta aaaggcatca cgactattga gacgactatc gatgaaatta
     4741 ttcaattaaa atcaggtgtc tgtcaggatt ttgcccattt gcttttacat attttgcgca
     4801 ctttacaaat cccttcccgt tatgtgagcg gatatatttg tcccaacaag agcggaatgc
     4861 gaggcgaagg ggcaacacat gcctgggtag atgtgtattt gcccaatttc ggatggatag
     4921 gtattgaccc tactaataat tgctatgcca atgtaaacca tattcaatta gccgtaggaa
     4981 gagattttgc agactgcact cctataaaag gcaccttcaa agggccggcc aatcaagatt
     5041 tattggtata tgtatcggta ggttacgaag acggaacggt atttgaagat acgaataccg
     5101 tattgatgca gaaagagcaa acacgcattg cgccggcttt attgatacaa caacatcagc
     5161 aatagtgaaa tattgtttgg tgtattaagc aagaatgctt atctctaaga aatgtaaatc
     5221 agtcattaat cgttatagat ggcatcaatt tctttagcgt acttatcatt aatgattttt
     5281 cttttcaact tcatcgtagg cgtaagctca cccgtgtccg gtgtccattc attagccagt
     5341 aatttgaact ttttaatttg ctcaatatgt gaaaaatttg gattcaactc atcaacaata
     5401 ctctgatatt ttgcaatcac tttcggattg tttatcaact cgtctttagt agaatacgtg
     5461 atagtgtttt ctttggcaaa ttgttctaat tgtgcataag caggaacaat cagtgcggaa
     5521 acaaactttt tgctttcgcc tactaccatg atttgctcta caaagaaatt ttccctgaat
     5581 ttgctttcga tgggtgcagg agcaacatat tttcctcctg atgttttcaa taattctttt
     5641 ttgcggtcag taattttcaa gaacttattt cccgcatagt tggtaacaaa cgtacccaca
     5701 tctccggtgg agaaccaccc gtcttctgtc agcacttcac gcgttttttc cagttcctta
     5761 taataaccca tcataatgtt gggtccttta gccaaaatct caccgtcttc ggctatcttc
     5821 acctccacat cttttatcgg catacctaca gtgccaagca tggctccgcc ttcagaaaaa
     5881 cggttaaatg taattaccgg agaagtttct gtaaggccgt aaccctcgcg gattggaata
     5941 cccatagctg aaaacactct ggctatttta agcggacatg ctgctgaacc ggttactata
     6001 ccttttactc tgccgccaag ccgttctctg attttgctga aaacaagttt gtcggcaatg
     6061 gtaaacatga atgatggttt ttgatcgtaa tcatatttgt ctgccaagtc cattgcccat
     6121 aaaaacaatt tctttttgat gcctgtagat tgcaaaccgc ccgtcatcac tttttcataa
     6181 actttttcca gtaatcgggg tacggtagta aagaagtgcg gttttacaaa tcctaattta
     6241 tccgacaagg tatcgatatc agagtaataa acattgatgc ctgcatacag ataagtataa
     6301 ataaccatgc gctcgaagat atggcaaagt ggtaaaaagc tcaaagcact ttgaccgggc
     6361 tcaatgggca gttcagcgcc aacagtcaat acattagata cgatattatt atgagaaagc
     6421 atcacccctt taggcactcc tgtagtacca gaagtataaa tcagcgtagc caatgaatct
     6481 ggggtgatgg tgtcagaaat cgctttaatt ttagaaacat catcgtcatg ccccagtgtc
     6541 ataatttctt cgtaattctt agctcccggc acgttatcaa aggtgtatat ttcttgcaaa
     6601 gaaggtactc tgtttttcaa attctttact ttttcaaata atttttcatc tgaaacgata
     6661 caaagcttag attcagaatg ttttaaaata tgttcataat cgctttcaga aatagtaggg
     6721 tatagtggca cattgattgc accgatttgt ccaatgccct gatcgcagat attccattct
     6781 gtacggttgg ctgatgtaat taccgcaatt ttatctccta cgtttattcc aagctttaaa
     6841 agtcccaaac taaatttatc cgatttctct atgagctcat ccgtactgca ctcccaccat
     6901 ttatcaaatt tcatcgatgc tattgcaatc ttttggggat gattttcttt ctggtaatgc
     6961 aaaaaatcaa aaagtcttct aacttccata aggtgtattt ttgttataaa tctgccggat
     7021 tagatccgaa tatttttctt taatgcgttt tcttctcaat ttcatggttg cggtgagcag
     7081 tccgttatca acagaccatt catcagccag tagctcaaaa tgcttaacct gttctacttt
     7141 tccaaagtta acatttaatt cggtaattat ttccttgtaa agttgttttg tttctgtaag
     7201 ttgtatcaaa tcttcaggag aattaaaggc taatttctct ttttttgccc atatatttaa
     7261 ttcttcaaaa ttaggcaata ctaaagcaga aataaatttt tccccgtcac caataagtga
     7321 aacattttct ataaaaaaag attctttcag tttattttct atcggagaag gtgctacata
     7381 ttttcctccc gaagtcttaa atagttcttt cttgcgatcg gttattttca gattttggtc
     7441 tgaagtaaat tcaccgatgt cgcctgtcaa caaccatccg tctttatcaa aggctttttt
     7501 tgtttcctct tcatttttgt aatagcccaa catcacatta ggccctttta ctaaaatctc
     7561 accatcatca gccagcttta cttctacatt ttccaatacc ctacccacac atcctgccct
     7621 gaaacactta cgcttaaatg gcaccacgct aacaaccggc gagcattccg tcagcccata
     7681 accttcgaga acaggaatgc cggcattggt aaaaatgttg gcatattttt tttgtaaggg
     7741 agccgaacca caaatgatgg ccctgattct tccgccaagg gcttgcctcc atttttcaaa
     7801 tactaatttg ttggctacat tcagattaga ctctaaaaag aaagatttcg gctcgcccaa
     7861 ttctgcactt tctgcttgtt tcacactcca gaaaaaaagc tgttttttga ttcctttcag
     7921 gtcatttcct tttttgatta ttttttcaaa aacacgttct aataaacgcg gaacggttgt
     7981 aaagtaatgc ggcttgatat cttgcaagtg ttcggaaatg gtttctaagc cgtctgcgta
     8041 ataaatttgt gctccggcag caagatagac atagtttatc gtgcgttcaa atacatgaca
     8101 aagcggcaag aaactcagca ccacacattt tgcttcaagt ggaatggctt tcaagcaagc
     8161 ttgcacattt gaaacaatat tttgatgcga cagcataacc cctttgggcg ttcccgttgt
     8221 accggaagta tagataatgg ttgctaaatc agttggttgt atactgttct ttatctcggt
     8281 aatctggcga atataaagac tctcgtctga cagtaaattt ttccagtgtt ctaccccttc
     8341 tatttcttca aatgcaaaaa cacctttgag cgtgggtatt ttgggtagca cctctcttat
     8401 tttgcgatgc atcagtttat cagagaggaa gacgtattta atttccgcat gtgtaaaaat
     8461 atattcgtat tccttagcgc ttattgtcgg ataaatggga acatcaatta cgccaatttg
     8521 ttgacaagct aaatctacaa aatgccattc aggtcggttg cttactgaga taatggcgac
     8581 tttatcgcct ttttgcaagc ccagtttcag caagccggca ctaagctgat tggcaagatg
     8641 aataaaatcg tgtgttgaat aacgtatcca gttttttttg tcttcacgac gggcaaaaca
     8701 agtatccagc ggataccatt gttgctgata atctataata tcgaaaatgc gggtataact
     8761 caaaacgtca atgtgaaaat caaaattaat aaaaataatc tgtatctata ctgaccttat
     8821 taaaacaaat tttgttctat attacttgat ttaggattaa gaaaaataag ccgataaaat
     8881 agagactctt gctctccctg tcccgcaact tcgggaaacc agcgaccctc taaaatgaat
     8941 tataaaaaga aaggttgcca attggcaacc tttcttgctc tccctgctgg acttgaacca
     9001 gcgaccctct gattaacagt cagatgctct a
//
LOCUS       DAOQII010000090         8957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4157_length_8957_cov_5.684341, whole genome shotgun sequence.
ACCESSION   DAOQII010000090 DAOQII010000000
VERSION     DAOQII010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_8957_cov_5.684341"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..1489
                     /gene="atpB"
                     /locus_tag="PLF48_05800"
     CDS             308..1489
                     /gene="atpB"
                     /locus_tag="PLF48_05800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNF48874.1"
                     /translation="MKRHISIFFFAFCLLLTSLSLAEEHTSTATPATTTIAEKPHEPI
                     ANEHATEAEHGNKDASSVVVEHIMNHISDANQFHVATVSGKHFVIALPCILYRNGKLS
                     VFSSSRLEHDQEYDGYKLDEHSGRVVDAVTGKRSTNLGTYFTSHDSSKFLDFSITKNV
                     FTSILGFIILAILLFAAKKAYTNRKGQAPKGLQSIIEPIVQFLVDDVIKPNIGPKYEK
                     FVPFLLSIFFFILINNLIGLIPFFPGGANVSGNIAFTMTLAVLAFLMININGSKSYWG
                     HIFAMPGVPKWVLVILTPVEIVGLFIKPIALMLRLFGNITGGHIAILSIVSLIFIFGS
                     LGKSIGGASLGGAMAIPIVLFVNAMELFVAFLQAYVFTLLTSIFIGAALEEHEHEHEH
                     H"
     gene            1520..1729
                     /gene="atpE"
                     /locus_tag="PLF48_05805"
     CDS             1520..1729
                     /gene="atpE"
                     /locus_tag="PLF48_05805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNF48875.1"
                     /translation="MTITGIAALGAGLAALGAGIGIGLIGGNATQAIARQPEASGDIR
                     GAMILAAALVEGAALIAIIFSFLMK"
     gene            1773..2285
                     /gene="atpF"
                     /locus_tag="PLF48_05810"
     CDS             1773..2285
                     /gene="atpF"
                     /locus_tag="PLF48_05810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNF48876.1"
                     /translation="MHILAFDAMELILPDLGLLFWTTVIFLLFWFIMGKFAFKPIVNA
                     IKDRERSIEEALSSADKAKEEMQKLTSDNEAMLKEAREESARILKEAKDTKEAIINEA
                     HNRAKDEAVKLIAEAKLEIDNQKKSALAEVKNEVSKLSLDIAQKVLGKELDAAKDQEA
                     YVQSLVEKLN"
     gene            2287..2826
                     /gene="atpH"
                     /locus_tag="PLF48_05815"
     CDS             2287..2826
                     /gene="atpH"
                     /locus_tag="PLF48_05815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNF48877.1"
                     /translation="MSTDRISIRYAKALFEQAVSSGSLEAIYADLNVVNNALKDSKDL
                     RNLIKNPIIKSDKKAEILNQIFSGKVSNDTLNFLNMVAKNARESYLQGMVGAFFNQYN
                     SLKNISEVTVTTAVELSPATETEIVRFIQKQTGNPNVKINKSLDASIMGGFIVNFGDK
                     VYDNSVRYKLEKIRKEIII"
     gene            2895..4472
                     /gene="atpA"
                     /locus_tag="PLF48_05820"
     CDS             2895..4472
                     /gene="atpA"
                     /locus_tag="PLF48_05820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNF48878.1"
                     /translation="MVEVRPDEISTILRQQLGNVSTEAQLEEVGTVLQVGDGIARVYG
                     LSNVKAGELVEFASGMRAIALNLEEDNVGVVLMGSSVEIKEGDTVKRTGLIASINVGE
                     GMLGRVVNTLGEPIDGKGPITGELFQMPLERKAPGVIFRQPVNEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKEFYDRGEPVYCIYVACGQKASTVANVAKT
                     LEEKGAMAYTVIVAANASDPAPLQFYAPFAGASIGEYFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLKRPPGREAYPGDVFYLHSRLLERAAKVINRDEIAANMNDLPPSLKGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEANLFNAGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFAKFGSDLDAATKAVLEKGKRNVEILKQPQY
                     SPVTVEKQIAIIYLGTKGLLKNVGIDKVRRFEDDFLTALEAKHPEVLAEFKKGGLSDE
                     STAKVEALAKDMESAYA"
     gene            4550..5437
                     /gene="atpG"
                     /locus_tag="PLF48_05825"
     CDS             4550..5437
                     /gene="atpG"
                     /locus_tag="PLF48_05825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNF48879.1"
                     /translation="MANLKEVRLRIASVKSTIQITKAMKMVSAAKLKRATDRIVQIRP
                     YSNQLNSILQNIASNLGDQMNSSYAQVRPIQKVLIVVITSDKGLCGGFNSNSVKAVKA
                     LLNEKYSDLAAANKVDILTVGKKGYDAFKAGKSRVNNDFNGLFHNLNFEDTSKAAEYI
                     MYAFDQKEYDVVEVVYGQFKNAGTQITTTEQYLPIKTTKAAKGSGVTKADYIFEPSQE
                     KILTDLVPKILKTQFFRYLLDSNASEHGARMTAMDKATENANELLRALNITYNRARQA
                     AITTELTEIVSGAAALSNG"
     gene            5516..6166
                     /gene="trmB"
                     /locus_tag="PLF48_05830"
     CDS             5516..6166
                     /gene="trmB"
                     /locus_tag="PLF48_05830"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNF48880.1"
                     /translation="MGRVSKSEKFELLKTHPLVMDKDPSAKGKWQTDFFKNNHPIIVE
                     LACGKGEYTLGMAKLFPEKNFIGMDIKGNRIWSSARLAQLDELTNVGYIRDQIDKLED
                     YFLPGEIDEIWITFSDPFPKKRDAKRRLTSNRFLPIYKKLLKTGGKIHLKTDSDILYE
                     FTKEVLATTPCIILKDYSDVYAMNKQEELFGIQTYYEKMHISDGKTIKYLCFSLVD"
     gene            complement(6163..7035)
                     /locus_tag="PLF48_05835"
     CDS             complement(6163..7035)
                     /locus_tag="PLF48_05835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF48881.1"
                     /translation="MATCLFCGNTHHKSSVFEDTVFNNKTFEYIKCSACGLIQVNPLP
                     NADDLNKMYPVEYQSEVTGTNDNRYGFLFEELEKVQLPVKKLLDYGCGAGQFLVEARD
                     RGYQAFGSEFNPDLVKQLAEKINGVQFVTIEEMLKPAYSESFDIILLNNVLEHLTNPK
                     PIMEILKSKLNPNGVFILTGPIENNFNLTQQSRKLIFSIRKNLFGKKASHTPTHIIYS
                     NRNNQLAFFKNLGLETLVFNISEGHWPYPSSLAQCTSTMQKAMYYIANTSVKMSKKFD
                     FWGNVFLYIGRKVD"
     gene            7125..8102
                     /locus_tag="PLF48_05840"
     CDS             7125..8102
                     /locus_tag="PLF48_05840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF48882.1"
                     /translation="MKKISIITPSYNQSAFIEQTINSVLSQNYANLEYIIIDGGSTDG
                     SVEIIKKYEKYLTHWVSEKDNGQSHAINKGFKMASGEIINWLNSDDYYTPDTFKIIAE
                     QMKDEQVMVLSGRSNVLQTNTIKYQTNGVDLYSDVVKTMAYARIDQPETFIKRKVLET
                     TGLINEQLHYLMDREWWLRYLFYFGSKQVKSIDAVLVNFRLHEASKTVSAAAGFEKES
                     LSLYYSIAKKYGLEEAAFFEKMPDVILIPLNHYPLDFPAETVRKIIHNYWLQQAGMAY
                     AADDYGTAKLFLNQIKTDLLVAENKSIYTSLKNRITYLPLFLKKIWNKR"
     gene            8102..>8957
                     /locus_tag="PLF48_05845"
     CDS             8102..>8957
                     /locus_tag="PLF48_05845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF48883.1"
                     /translation="MEISIIIPTFNRETVLKQTLDNLKPLVEKYPIEIIIVNDGEALK
                     DNYTDENIHVFQNPKKGVTTARNFGAAKAKSNVLFFLDDDMWCNEDAVKAILSFKERG
                     LTNTSCFLMNWEYPAELKEKLSKEKIGRYLLNANYHTLEGRLMDKVDNTLEFKQIDSI
                     GSCSFVISKQLFAQIGGYDESYIFQGEDIAIAQTINRHRIPILLSTQITCFHNHIDRL
                     DVEGMMDRDYRGYYSQFLQQGNNAVNSTKTIFYNAFLHIQNLIIKTYDLIPNSPAYDK
                     LTFRFLGLI"
BASE COUNT         2994 a         1450 c         1799 g         2714 t
ORIGIN      
        1 gagtctgtta ctgtgcatga agctaagatt ttacctggcg ccggtactac tctttctggg
       61 tctaaagtgg atagtattta ctttgactta gaattttctg atgatccggg ttatcactat
      121 ttcttctctg gtattaaata tactggtcac gttgaagatg agtatcatta attaatttta
      181 atactaaatt gtaaaagagg ctgatcatca gcctcttttt ttgtttctac ttaacacaca
      241 tttaatttat ccctacatct catttagcct ctttatttgc tatctttgca ctgcattttt
      301 taagcacgtg aaacgccata tttccatatt ctttttcgcc ttttgtttac tactgacatc
      361 tttgtcatta gcagaagaac atacttcaac agctactcct gctacaacaa ctattgccga
      421 aaaaccacat gagcctatag ctaacgaaca tgctacagaa gctgaacatg gcaataaaga
      481 tgcatcttca gtagtggtag aacatatcat gaaccacatc tctgatgcta accagtttca
      541 tgttgcaaca gttagtggaa agcattttgt tatcgcttta ccttgtattc tctatagaaa
      601 tggaaaacta tccgtatttt cttcatctcg tttagaacat gaccaagaat atgacgggta
      661 taaactggat gaacactctg gtagagtggt agatgcagtt acaggtaaaa gaagtacgaa
      721 tttaggtacg tattttactt ctcatgattc gtctaagttt ttggattttt cgattactaa
      781 aaatgttttc acatcaatat tgggtttcat catacttgct attctgttat ttgctgctaa
      841 aaaagcatat acaaacagaa aaggccaagc tccaaagggc ttacagtcaa taattgagcc
      901 gattgtacag tttttagtag atgacgttat taaacctaat attggtccga aatacgaaaa
      961 atttgtacca tttttattgt ccatcttttt ctttatcctg attaataatc ttattggctt
     1021 aattccgttt ttcccgggtg gggcaaatgt atcagggaat attgccttta cgatgacctt
     1081 ggctgtactg gcatttctga tgattaatat taatggctct aaaagttatt ggggacacat
     1141 atttgcaatg ccgggtgtgc caaaatgggt tttggttatc ttaactcctg tcgagattgt
     1201 cggattattt atcaaaccaa tcgccttaat gcttcgtttg tttggtaata taaccggtgg
     1261 acatattgcc atattgagta tcgtgagttt gatttttatt tttggcagtc ttggcaaaag
     1321 tattggaggt gcatcattgg gtggtgctat ggcaattcct atcgtattat ttgtaaatgc
     1381 tatggaatta tttgtggcat ttttacaagc ctatgtattt actctgttga cttctatatt
     1441 tataggagca gctcttgaag aacatgaaca tgaacacgaa catcattaat taaattaata
     1501 aatcaaaaaa aattaagtta tgacaattac aggaatagca gcgcttggag caggtttagc
     1561 agcattaggc gcaggtatcg gtatcggttt aatcgggggt aatgctacac aagcaatcgc
     1621 tcgtcagcct gaagcatctg gagatatcag aggtgcgatg attttggcag cagcattagt
     1681 agaaggtgca gctcttatcg ctatcatctt ctcattcttg atgaaataat ttcgcgaaag
     1741 cactaacgtc gtaagacaca aaatatattg ctatgcatat attggcattt gatgcaatgg
     1801 aattaattct gcccgatttg gggttgttgt tttggacaac agtaatcttt ctattgtttt
     1861 ggtttatcat gggaaaattc gctttcaaac cgatagtgaa tgccattaaa gatcgtgaac
     1921 gctctatcga agaagcttta tcttctgccg acaaggctaa agaagaaatg cagaagctga
     1981 cttctgataa cgaggctatg ctgaaagaag cccgtgaaga aagtgcgcgt atcttaaaag
     2041 aagctaaaga cacgaaagaa gctatcatta acgaagccca taacagagct aaagatgagg
     2101 cagtaaaatt aattgcagaa gctaagctgg aaatcgataa tcaaaagaaa tcggctttgg
     2161 cagaagtaaa aaatgaggta agtaaattgt ctttggatat tgctcagaaa gtattgggaa
     2221 aagaattaga tgctgccaaa gaccaagaag cttatgttca atctttggtg gagaaattaa
     2281 actgatatgt ctacagacag aatatccata cgatacgcga aagcattgtt tgagcaagcg
     2341 gtaagttccg gttcgcttga agccatttat gccgatttaa atgtcgtgaa taatgcgctc
     2401 aaagacagca aggacttgcg taatttgata aaaaatccta ttataaaatc agataaaaag
     2461 gcggagattt taaaccagat attttcagga aaagttagca acgatacctt aaactttctg
     2521 aatatggtgg ctaaaaatgc cagagaatct tatttacaag gaatggttgg ggcgtttttt
     2581 aatcaataca attcgttgaa aaacatttcg gaagtaacag ttacgacagc cgtagaatta
     2641 tcaccggcta cagaaactga gattgttcgt tttatccaga agcaaacagg aaatccgaat
     2701 gtaaaaatta acaaaagcct cgacgcttct attatgggtg gttttattgt caactttggt
     2761 gacaaagtgt atgacaactc agtgcgatat aaattagaaa aaattagaaa agaaatcatt
     2821 atataatgtt agatgaaaaa tgattaatgt ttaattattc aacattcaac attcaacatt
     2881 aaaaattaat taaaatggta gaagtaagac cggatgaaat atcaaccata ttgcgtcagc
     2941 aattaggtaa tgtatccact gaagcacaac tggaagaggt gggtactgtt ttgcaagtgg
     3001 gagacggtat cgcacgtgtt tatggtttgt caaacgtaaa agcgggtgaa cttgtagaat
     3061 ttgcaagcgg tatgcgtgcc atcgcattaa atttagaaga agataatgta ggtgtggtat
     3121 tgatgggttc ttctgtagaa attaaagaag gagataccgt gaaacgtacc ggattaattg
     3181 cgtccattaa cgttggcgaa ggtatgttgg gacgtgttgt aaatacactt ggcgaaccta
     3241 ttgacggcaa aggtccgata acaggagagt tatttcaaat gccattagaa agaaaagctc
     3301 cgggtgtaat tttccgtcag ccggtaaatg agccgttgca aacaggtatt aaagcaattg
     3361 atgcgatgat tccgataggt agaggtcagc gtgagttaat tatcggtgac cgtcaaactg
     3421 gaaaaaccgc tatcgctatt gataccatta tcaaccaaaa agaattttat gaccgtggcg
     3481 agccggttta ttgtatatat gtagcctgtg gtcaaaaagc ttctacagtt gcaaatgtgg
     3541 ctaaaacact agaagaaaag ggtgcgatgg cctataccgt tattgtagca gctaacgctt
     3601 ctgaccccgc tccacttcaa ttttacgcac cgtttgcagg tgcatctatt ggggagtatt
     3661 tccgcgatac aggtcgtccg gcattgatta tttatgatga tttgtcaaaa caagctgttg
     3721 cttatcgtga ggtatctttg cttttgaaaa gacctccggg acgtgaagct tatccgggtg
     3781 acgtatttta cttacactct cgtctattag agcgtgctgc taaagtgatc aatagagatg
     3841 agattgcggc aaatatgaac gaccttccac cttctttgaa aggaaaagta aaaggtggtg
     3901 gttctttaac ggcattgcct attattgaaa cacaagccgg cgacgtttct gcctatatcc
     3961 caacaaatgt aatttctatt acagatggtc agatattctt ggaagctaac ttgttcaacg
     4021 caggtgtgcg ccctgctatc aacgtaggta tttctgtatc acgtgtggga ggtaatgcac
     4081 aaattaaatc gatgaaaaaa gtagcaggta ccttaaagct ggatcaagca caatacagag
     4141 agttggaagc ttttgctaaa ttcggctctg atttggatgc cgcaacaaaa gcggtattgg
     4201 aaaaaggaaa acgcaacgtg gaaattttga aacaaccgca atattctccg gtaaccgtag
     4261 aaaaacaaat tgctattatc tatttaggta caaaagggtt gttgaaaaat gtgggtattg
     4321 acaaagttcg ccgatttgaa gatgatttct tgacagcttt ggaagccaaa catccggaag
     4381 tattagcgga atttaaaaaa ggcggcttaa gtgatgagtc tacggcaaaa gtagaagcac
     4441 tggctaaaga tatggagagt gcatacgcat aggaaattgt aagttttgaa ttataaattt
     4501 tgaatagcat cagtttgtag tttaaaatct aaaatctaaa atttcaaaag tggcaaattt
     4561 aaaagaagta cgattacgaa tagcatctgt aaaatcaaca atacagatta ctaaagccat
     4621 gaaaatggta tctgcggcta aactaaaaag agcaacagac cgtatcgtac aaattcgccc
     4681 atactctaac caattgaatt caattttgca aaatattgcc tctaatctcg gcgatcagat
     4741 gaattcatcc tatgcgcaag taagaccaat tcaaaaagta ttaattgttg ttatcacttc
     4801 tgataaaggt ctttgtggcg ggtttaattc caactctgta aaggcagtaa aggcattgct
     4861 gaatgaaaaa tattcagatc tggcagccgc aaacaaagta gatattctga cagttggcaa
     4921 gaaaggatat gatgctttta aagcaggaaa gagtcgtgta aataatgatt ttaacggttt
     4981 gtttcacaac ctgaattttg aagatacttc taaagcagct gaatatatca tgtatgcttt
     5041 tgaccaaaaa gaatacgatg tggtggaagt tgtttatggt caatttaaaa atgcaggaac
     5101 tcaaattaca acaacagagc agtatctgcc aatcaagaca actaaagcag caaaaggaag
     5161 cggcgtaaca aaagcagatt atatttttga gccttctcag gaaaaaatat tgacagattt
     5221 agtaccaaaa atcttgaaga cacagttttt cagatatttg ttggattcaa atgcttcaga
     5281 acatggtgca cgaatgacag ccatggataa agcaactgag aatgcaaacg agttattgcg
     5341 tgctttaaat attacctaca accgcgcacg tcaggctgca attacaaccg aattgacaga
     5401 gatagtttca ggtgcggctg ctttgagcaa cggctaagat ttttatctgt ttttagttgg
     5461 ttgaaatcag ctaacaaact ttactattta tcagcattta ttttaaaaat ttcatttggg
     5521 tcgcgtttct aaatctgaga aatttgaatt actcaaaacc catcctttag tgatggataa
     5581 agacccgtct gctaaaggaa agtggcaaac ggatttcttt aaaaacaatc atccgataat
     5641 tgttgaatta gcctgcggca aaggggagta taccttaggt atggcgaaat tatttccgga
     5701 aaagaatttc atcggaatgg atattaaggg caacagaatt tggagctctg cccggttggc
     5761 acaattagat gagcttacca atgtgggcta tatccgcgat cagattgata aactggaaga
     5821 ttattttttg cctggtgaaa ttgatgaaat ctggattaca ttttctgacc cgtttcccaa
     5881 gaaacgagat gctaaacgtc gattaacttc caacagattc ttgcccatct ataaaaagtt
     5941 attaaagacc ggagggaaaa ttcatttaaa aacagacagt gatattttgt atgaatttac
     6001 aaaagaagta ttagcaacca ctccatgtat aatactaaaa gactatagcg atgtatatgc
     6061 tatgaataag caggaagagc tatttggcat acaaacctac tatgaaaaaa tgcacatatc
     6121 agacggcaag acaataaaat atttatgctt cagcctggtt gattaatcaa ctttccgacc
     6181 gatatataag aagacgtttc cccaaaaatc aaatttctta ctcatcttaa cagaagtatt
     6241 tgcaatataa tacatcgcct tttgcatagt ggatgtacac tgagccaaac tggacggata
     6301 aggccaatgc ccttcggata tattgaatac cagtgtttct aatcctaaat ttttaaagaa
     6361 cgctaattga ttatttctat ttgagtaaat aatatgcgtt ggtgtatgac ttgctttttt
     6421 accaaacaaa tttttgcgaa tagagaaaat aagttttctg gattgctgag taagattaaa
     6481 attattttcg ataggaccgg ttaaaatgaa aacaccattc ggattgagtt tgctcttcaa
     6541 aatttccata atgggttttg gattggttaa atgttctaag acattattca ataaaataat
     6601 gtcgaatgat tcagaataag ccggtttcag catttcttca atggttacaa actgcacgcc
     6661 atttattttc tcagcaagct gtttgactaa atcaggatta aattcagaac caaatgcttg
     6721 gtagcctctg tctcttgctt caacaagaaa ttgaccggct ccgcaaccat aatccagcag
     6781 tttcttaacg ggtagttgta ctttttccag ttcctcaaaa agaaatccgt atctgttatc
     6841 gtttgttcct gttacctcac tctgatactc tacgggatac attttgttta aatcatctgc
     6901 attgggtaat ggattcactt gtattaatcc gcaagcagaa cattttatat attcaaatgt
     6961 tttgttgttg aaaacagtgt cttcaaatac agaagattta tgatgcgtat tgccgcaaaa
     7021 caaacaagtt gccatgtaat aatttgtatc aaagataatt tttctttttg agctttattg
     7081 aggaaagtaa atttcgtgga agagaatgat tatttttata gcggatgaaa aagatttcca
     7141 tcattacacc atcttataat cagtcggctt ttattgaaca aacgattaat tcggtattgt
     7201 cccaaaatta tgcaaattta gaatacatta taatcgatgg cggaagtaca gatggaagtg
     7261 tggaaattat caagaaatat gaaaagtatt taacgcattg ggtaagtgaa aaagacaatg
     7321 gacaaagtca tgccattaac aaagggttta aaatggcaag tggagaaatc atcaactggc
     7381 taaacagcga tgattattat acacctgata cgtttaagat tattgctgag cagatgaaag
     7441 acgaacaagt catggtattg agtgggagaa gcaatgtgtt gcagacaaat acgataaaat
     7501 atcaaaccaa tggagttgat ttatatagtg atgttgttaa aacaatggca tatgccagaa
     7561 tagaccagcc tgaaactttc ataaaaagaa aagttttgga aactacagga ttgataaatg
     7621 aacaattgca ttatctgatg gatagggagt ggtggttgcg ctatttattt tattttggaa
     7681 gtaaacaagt gaaatccata gatgcagttt tggttaattt caggttgcat gaagcatcca
     7741 aaacggtaag tgctgccgca ggatttgaaa aagaatcgct cagcttgtat tattccattg
     7801 ccaaaaaata cggattagaa gaagcagcct tttttgaaaa aatgccggat gttattctca
     7861 ttccgcttaa tcattatccg cttgattttc cggcggagac agttagaaaa ataattcata
     7921 actattggtt gcaacaggca gggatggctt atgctgctga tgattatgga actgcaaaat
     7981 tgtttttaaa tcaaataaag acagatttat tagtagcaga aaataaatca atttacacat
     8041 cacttaagaa caggataaca tatctacctt tgtttttaaa gaaaatttgg aataaacgct
     8101 aatggaaatc agcatcatta tacccacttt caatagagag actgttttaa aacagacact
     8161 ggacaatctt aagcccttgg tcgaaaaata cccgattgag ataattattg taaacgatgg
     8221 tgaagcgtta aaagataatt atacagatga aaatatacac gtatttcaga atcccaaaaa
     8281 aggggtaacg acggcaagaa actttggagc ggctaaagcc aaaagcaatg ttttattctt
     8341 tttggatgat gatatgtggt gtaatgaaga tgctgtaaaa gcgatattaa gctttaaaga
     8401 aagaggcttg acaaacacca gctgtttcct catgaattgg gaatacccgg ctgaactgaa
     8461 agagaaacta agcaaagaaa aaataggcag atatttattg aatgcaaatt atcatacttt
     8521 ggaaggcaga ttgatggata aagttgacaa tacgcttgag tttaagcaaa ttgattctat
     8581 agggagttgc tcatttgtaa tcagtaaaca gctatttgca caaataggag gctacgacga
     8641 atcatatatt tttcaaggag aagatattgc aatagctcaa acaataaaca gacatcgtat
     8701 tcccatatta ttgtcaactc agataacatg ttttcacaac catattgaca gactggatgt
     8761 tgaaggtatg atggacaggg attacagagg atattactcg cagtttcttc aacaagggaa
     8821 taatgcggta aattcaacta agactatttt ttacaatgca tttctacata ttcagaattt
     8881 gattattaag acctatgatt taattccaaa tagtccggcg tatgataaat taacgttcag
     8941 gtttttaggg ctaattg
//
LOCUS       DAOQII010000091         8764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4281_length_8764_cov_6.854174, whole genome shotgun sequence.
ACCESSION   DAOQII010000091 DAOQII010000000
VERSION     DAOQII010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_8764_cov_6.854174"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLF48_05850"
     CDS             complement(<1..463)
                     /locus_tag="PLF48_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48884.1"
                     /translation="MDWGLGHATRCIPLIDSLIMQQHDVMLAGSGTSLQFLRDAYPTL
                     EVVELPAYDIYYPEGTNASFQVLLQTPKVLRAIKKERSLIAEIYAVYKWDYIISDNRY
                     GVLHPLARNIFICHQIAIRAPKLFSWTNPFFLSLHLRFIKPFHELWIPDIKG"
     gene            complement(504..1934)
                     /locus_tag="PLF48_05855"
     CDS             complement(504..1934)
                     /locus_tag="PLF48_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48885.1"
                     /translation="MENSKIFNTLLALNKWEKKSFEKFVQSPYFNSSEDIVRLLQYLY
                     TLIETDSPLPEKKELYKKIYQKQAYNDQKLRLNMSSLFKLLEKFLLMQQIDEDAFLQK
                     LKLMSAYNHHNHHKNFHRTYEDATHLLEKQPLRNANYYENYYDIEVERYIEKRNLSRT
                     SDYNFQMLINNFDIYVIAEKLKQGCILISHEQVNNVDYNYGIFDIVLEHVEKNKLLDV
                     PVIAIYYYIFNTFKHAEKEENFEKLVALIKQNLHLFPKLEARDVLIYANNYCIKMNNM
                     GKEGYARKSFDINKIGIETGAFLEKGFLSRFTFKNIVTVGIRLKEYKWTEKFIEDYKD
                     LLEEKYQESSYSYNKAFLEYERKKYDIAITLLQKADYDDLLLNLSAKNILMKIYYELD
                     YIEPLEYLIGSITALLKRKKLEQIYKNNYLNICKLTRRLISLKTMTSSQRSYINKKKE
                     VTAKIIKETNPCFEKKWLLEQLDKIK"
     gene            2053..4983
                     /locus_tag="PLF48_05860"
     CDS             2053..4983
                     /locus_tag="PLF48_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48886.1"
                     /translation="MSRKYLFIFLLLSLLFPLNPVLASGFVKLLYGIAPTSVLEGKNK
                     SAIVLTETFYGYHPIYFLQETDSMGNVIQLVDLSTIQDTWLQGSVDIWLTQNKDIFAV
                     SHFKDDMGNLTQKISKVQNGSIVFTKNIINSDTLQPMLVRSYANTTDNGFIQLLSNKN
                     YTQYVMQKINNSGDVVYTKLIPSSFFNTGLDTINLFGLKHELRITEHEGKYYISGIGI
                     NSLLNKYQLFQTIYNPQTDDFQYNRYALPEIAVNNQAQPVLFNECFLNSGAMRAIIMH
                     YDSTSALNTYYIEDLHTENGLKDLVYTYQDSSNNANFAQRLNFDKSGNLYFLKPNSLS
                     KRDANGNLKWNRVLFPDRIASNQPITDDFIFMRNFTQLSDSGFYIIGKGGRALNQIFC
                     FCLGVIIKTDKEGIVYRRQISGNIYADANNNCVPDNENGIKNTLVTAYNANASYFGIT
                     DTLGNYKIPVENGSYTVKAFLPDSIYWQACTPAVQINTLTDTTKVDFALQRRVSCPLL
                     QTDISIPFLRRCFENSYTIHYCNYGTATAQNAVIKVTFPTEIEVRNTGIPWSSATDNT
                     FTFPIGNLNEQDCGSFTITAYLSCGDSSALGETHCVEAHIFPDSLCLVPDSRWDGSEI
                     TVSGICLGDSVQFTVSNVGSGDMRNPNISYVLEGDFLRIAPVYFQLNAGQSQTFTFAA
                     DGTTQSFIAAQSAYYPFGGMATASVEDCGGFTPGYVTHFTQENNSPFLVKNCTQNIGS
                     YDPNDKTALPVGLDTLHYIAPSTAIQYIIRFQNTGTDTAFNIILEDSLSPLLDISTFA
                     VGGSSDPYTYSIEQNGLLRIVFPNIMLPDSNKNLAGSMGYFSFSIKPKATLPEYTRVE
                     NNAIIYFDFNEGVKTGMVFHTIKNNLYQFIADYINEPSNKINYVIYPNPFFDQFTLTL
                     SDKQIQNKILQLRLYDLDGKEIYRSEIKSGVPVLVSSLDRNIYFFTISHGNKIISTGK
                     IIKQ"
     gene            5119..8091
                     /locus_tag="PLF48_05865"
     CDS             5119..8091
                     /locus_tag="PLF48_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF48887.1"
                     /translation="MMRFFTKNCFVLVMYLSWLCSYAGDMPKHYQPKAFSYSSQIIST
                     ADNGFLMLSYYKNSTAEPYTPVITKFDQKADVQWEKSLSPIQLLDFSGVDEGRLFPMQ
                     DGYLVFHTYPSVGNNGRLGLVQRFDKNWNLVYVSVPSLDSIGFDETYLNGWNWSMDKI
                     QPLSDGGYMVIGTNLADCYNKGVVFARYSPIGTLVWKHKSPITNSCIQFRYDQALLHI
                     TSDNKLLLGIGNRYQMTATTYGASFQLNKKTLNDNEIWSKTVITTLDVTQIANKNVLL
                     CQKELANGNLKYLAVYDINGGDYAHGAFLQYTISGTDGTILQIDTFAFNLPLANDENF
                     RRVEIDDEENILILGQKSFTKLDKKGNLIWQRTPFEDIRLFDVLGTKYHLTSFTTTSN
                     GNYLLTGNNLQTTNANSSGIYFFLITADGQSKVKALTGKVYIDIDKSCTYTNNETGYP
                     GLQVVAERNGQRHYASTDTSGTYYMPLDSGIYNITVQLPNSNVWKMCYQSYMANLTVN
                     QPLSFLNIPLYPKVTCPFLNVEIGTPYLRKCAENYYTLYYCNTGNDTAHSTYIDVQLD
                     ADMSFTHADLSSINLGNNQYRFQLGNLNPQNCGSFKIYAQLSCDAIAGKTHCTSAHIY
                     PDNACPPVASQWDGSDIKLYGYCQQDSAIIVIKNTGNNMQSPRHYIVIEGDFLRIQPQ
                     NYQLAANDSIVLRLSADGQTIRVSAQQDPNFPYPSQPSITIESCSGVIDSTGLVNQFP
                     HDDAAAFISTSCVQNRSSFDPNEKMAQPVGYLQEHYVEKNTELEYTLRFQNMGNDTAF
                     FVELLDTLSTGLDLKTLVMGASSHPYNYAVYGGHILQINFPGIKLPDSLTNEPMSHGF
                     VTFHIQPKSTIPNGTLIENNAQIYFDYNSAVTTNTIYHTVDSIRYKIVSLVNYHPTIN
                     KIAVYPNPFSTFAHIEINSSQPLKNTTVQVWSIDGKLILNQPLEKDIDGRLLTEGMYI
                     LKILAEGKLLATAKLIKQ"
     gene            8129..>8764
                     /locus_tag="PLF48_05870"
     CDS             8129..>8764
                     /locus_tag="PLF48_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48888.1"
                     /translation="MCDTFVYLPESIEEPVIFGKNSDREPDEAQGIVHYPNYSTSSKS
                     QRTTYIEVETDAQKLEVYLSKPFQLWGAEMGVNQYGVAIGNEAVFTKLPIEKKNTGLT
                     GMDLLRLALERAITAKEALENIILFLEKYGQNACGGYRDKKFYYHNSFIIADAHSAFV
                     LETAGTFWVYQKIKGFRAISNGLSIEQEFDGIHPDAIAHAHKKGWVKKQQAF"
BASE COUNT         2799 a         1611 c         1570 g         2784 t
ORIGIN      
        1 ctccttttat atccggaatc caaagctcat ggaatggctt gatgaaacgc aagtgcaagg
       61 ataaaaaaaa tgggtttgtc catgaaaata attttggcgc cctgatagca atttgatggc
      121 aaataaaaat gtttcttgca agcggatgaa gaacaccgta acggttatct gatataatat
      181 aatcccactt gtacactgca tatatttcag caatcaagct acgttctttt tttatggctc
      241 gcaatacttt aggtgtctgc aacaacacct gaaatgaagc gttggtgccc tccgggtaat
      301 aaatatcata ggctggcagt tctacaactt caagagtagg gtaagcatct cttagaaatt
      361 gtaatgacgt gccactgccg gcaagcataa catcatgctg ttgcatgatt aaagaatcaa
      421 tcaagggtat gcaacgagtg gcatgaccaa gtccccagtc caaaggagca atgagtattt
      481 tttttacaac gctaataatt caactattta attttatcca gttgctcgag cagccatttt
      541 ttttcaaaac acggattcgt ttctttaata atctttgccg ttacttcttt tttcttgttg
      601 atgtagcttc gttgtgaaga ggtcatcgtc ttcaaactga ttaatcttct ggtaagcttg
      661 caaatattaa ggtaattgtt tttataaatc tgctctaatt ttttgcgttt cagtaatgct
      721 gtaatactac caatcaggta ttccaaaggt tctatataat ccagctcgta gtaaatcttc
      781 atcaggatat tttttgcaga aagattcagc aataagtcat cgtaatctgc tttttgcagc
      841 aaggttatcg caatgtcata tttcttcctt tcatactcta agaatgcttt attataactg
      901 tacgagcttt cctgatattt ttcttcgagt aagtctttgt agtcctcaat aaatttttcc
      961 gtccacttgt attctttcaa ccgtatgcct actgtcacga tatttttaaa tgtaaaccgg
     1021 gacaaaaatc ctttttctaa aaacgctccg gtttcaatgc cgattttatt aatatcaaat
     1081 gatttacggg catatccctc tttgcccata ttattcatct ttatgcaata attattggcg
     1141 taaatgagaa catcgcgtgc ttccagcttt ggaaacaagt gaagattttg tttaattaat
     1201 gcaaccagtt tttcaaagtt ttcttctttt tcagcgtgct taaacgtatt gaaaatatag
     1261 tagtaaatgg caatgacagg gacatccagc agtttgtttt tttctacgtg ctccagcaca
     1321 atatcgaaaa taccgtagtt gtagtccaca ttgtttacct gctcatgcga aatcaggatg
     1381 cagccttgtt ttaatttctc agcaattaca tagatatcaa aattattgat gagcatttga
     1441 aaattataat ctgaagtacg gcttagattt cttttttcta tataccgttc tacctctata
     1501 tcatagtaat tttcgtaata atttgcatta cgtaggggtt gtttttcaag taagtgcgtt
     1561 gcatcttcgt atgttctgtg aaaattttta tgatgattat gatggttata cgcactcatc
     1621 aatttcagtt tctgtaagaa agcatcttcg tcaatttgct gcatcagtaa aaacttttca
     1681 agcaatttaa acaaactgct catattcagc cgcaattttt gatcgttata agcctgcttt
     1741 tggtatattt ttttgtaaag ctctttcttt tcaggtaatg gactgtcagt ttctatgagg
     1801 gtatacaaat actgtaataa tcttacaata tcttcagagg agttgaagta gggtgattgt
     1861 acaaattttt caaacgattt cttttcccat ttatttaaag ctagtaatgt gttaaatatt
     1921 ttgctattct ccatgtgttc taatttattg ctattcagta atttgcgatg tattattaaa
     1981 tacaacccca ctacaaacat agtaatttat cttaagattt taatgtaaaa atttataatt
     2041 ttatccttgt gtatgagtag aaaatatctt tttatttttt tgttattgag tttattattt
     2101 ccgctaaatc cggttttagc ttcaggtttt gtaaaactct tgtatggaat agccccaaca
     2161 tcggtgcttg aaggcaaaaa caagagtgcc atcgtattga cggaaacatt ttatggttat
     2221 catccaattt attttttaca ggaaacggac tccatgggaa atgtcattca gttggtagat
     2281 ttgtcaacga tacaagatac ctggcttcaa ggaagtgtgg atatatggtt aacacaaaat
     2341 aaagacattt tcgcagtgag ccacttcaaa gatgacatgg gaaatttaac tcagaaaatc
     2401 agtaaagtac aaaatggcag catagtattt accaaaaaca tcatcaacag cgatacatta
     2461 caaccgatgt tagtgcgttc ttatgcaaat actacggata acggctttat tcaattactc
     2521 agcaataaaa actatacgca atatgtaatg caaaaaatca acaattcagg tgatgtcgta
     2581 tatactaagc tcataccttc ttcatttttt aatactggct tagacaccat aaatttattc
     2641 gggttaaaac acgaattacg tatcacagaa catgaaggaa aatattatat atcaggcatc
     2701 ggaataaaca gtttgctgaa taaatatcaa cttttccaga caatatataa tccacagact
     2761 gatgattttc aatataaccg atatgcactt ccggagattg cagtaaataa tcaggctcag
     2821 cctgttttgt ttaatgagtg tttcctgaac tcgggtgcta tgagggctat cattatgcac
     2881 tatgattcaa ctagcgcatt aaacacctat tatattgaag acttacatac tgaaaacgga
     2941 ctaaaggatt tagtttatac ttatcaggac tcaagcaata atgcaaattt tgcccaacga
     3001 ttgaattttg acaaaagcgg taatttgtac tttcttaaac ctaactcctt atctaaacga
     3061 gatgctaatg gaaatctaaa atggaacaga gtgctttttc cggatagaat tgcttccaat
     3121 caacccataa ctgatgattt tattttcatg cgcaacttta ctcagttatc agacagcggg
     3181 ttttatatta ttggaaaagg gggcagagca ttaaatcaga ttttttgttt ttgtttaggc
     3241 gtgattataa agacagacaa agagggcatt gtttacagaa ggcaaatctc gggcaatatt
     3301 tatgccgatg caaataacaa ctgtgtaccg gataatgaaa atggaataaa aaacacactg
     3361 gtaactgcct ataatgcaaa tgcatcctat tttggcataa cagacacttt ggggaattat
     3421 aaaattccgg ttgaaaacgg aagttatacc gtaaaagctt tccttcccga ctccatctat
     3481 tggcaagcat gtacacccgc tgttcaaata aatacactta ccgataccac caaggttgat
     3541 tttgccctgc aaagaagagt cagctgtcca ttattgcaaa cagatatcag cattccgttt
     3601 cttcgccgat gttttgaaaa ctcatatact atacactatt gtaattatgg aacggcaaca
     3661 gctcaaaatg cagttataaa agttaccttt ccaacggaaa tagaggtgag aaatacaggc
     3721 attccatgga gttcagcaac agataatacc tttacttttc caataggaaa tttgaacgaa
     3781 caagattgtg gctcattcac tattacggct tatttatctt gtggagattc gagtgcattg
     3841 ggcgagaccc attgcgtgga ggctcatatt ttccccgaca gtttatgtct tgtacctgat
     3901 tctcgctggg atggttcgga aattacggta agcggaatat gtttgggcga ttctgtacaa
     3961 tttacagtaa gtaatgtggg atcgggagat atgagaaatc ccaatatttc atacgtgctt
     4021 gaaggagatt tcttgcgtat tgcgcctgtt tattttcagc ttaatgcggg tcagtctcaa
     4081 acctttactt ttgctgccga cggcactaca caatccttta ttgcagcaca gtcagcctat
     4141 tatccttttg gcggcatggc tactgccagc gtagaagact gtggtggctt tacccccggt
     4201 tacgttacac attttacgca agaaaataat tcgccgtttt tagtaaaaaa ctgcacacaa
     4261 aacataggtt cctacgaccc caatgacaag acagccctgc ctgtcggact tgatacgcta
     4321 cattatatag caccatcaac cgctatacaa tacataattc gttttcaaaa tactggaacc
     4381 gacacggctt ttaatataat cctggaagat tctttgtcgc cacttctgga tatcagcact
     4441 tttgctgtcg gaggcagctc agatccatat acttattcaa ttgagcagaa tggactattg
     4501 cgcattgtct ttccaaatat tatgcttccg gattcgaata agaacctagc cggttcaatg
     4561 ggttattttt cttttagtat aaaacccaaa gccacattgc cggaatacac aagagtggag
     4621 aacaatgcaa tcatttattt tgattttaat gaaggtgtga aaaccggaat ggtgtttcat
     4681 accataaaaa ataatttata tcagttcatt gctgattaca taaatgagcc atcaaataag
     4741 ataaattatg tcatttatcc caatccgttt ttcgatcagt ttacactcac attatcggat
     4801 aagcaaattc aaaataagat actgcaactt cgcttgtatg acttggatgg aaaagaaatc
     4861 tatcgttccg aaatcaaaag cggagtacct gttttggtgt cttcattaga tagaaacatc
     4921 tattttttca ccatatctca tggaaataaa ataatctcta cgggtaaaat cattaaacag
     4981 tagctacttt ttttatcata aataattgat agaaaagcta ttagattaat attttagaaa
     5041 ataagcaaca atactttcca ttttttgtat ttattttttt agaaaatatt tactaatatt
     5101 gagtgtattt ttatatttat gatgcgcttc tttacgaaaa actgttttgt attagttatg
     5161 tatttgagtt ggttatgctc atatgcaggt gatatgccca aacattacca acccaaagct
     5221 tttagttatt ctagccagat tatctcaacg gcagataatg gctttttgat gttgtcttac
     5281 tacaagaaca gtactgcaga accctacact cctgtgatta ccaagtttga ccaaaaggca
     5341 gatgtacaat gggaaaaatc attaagtcct attcagttgc tagatttctc aggtgtagac
     5401 gaaggacgct tatttcctat gcaagatggt tatttggttt ttcatactta tccgtcggtt
     5461 ggaaataacg gcagactcgg tttagtgcaa cgtttcgaca aaaactggaa tttggtctat
     5521 gtttccgttc cgtcattaga cagtataggt tttgatgaaa cgtatttaaa cggatggaac
     5581 tggagcatgg acaagattca acccctgagt gatggcggat atatggttat cgggacaaac
     5641 cttgctgatt gttataataa aggagttgtt tttgccagat attcacctat tggtactttg
     5701 gtttggaaac ataaatctcc aatcaccaat tcctgtattc aattccgata cgatcaggca
     5761 ttactccata tcacttcaga caataaatta ttattaggta tcggcaatcg ttatcaaatg
     5821 accgctacaa catatggcgc aagttttcaa ttaaacaaga aaacgctaaa tgataatgag
     5881 atttggtcaa agaccgtaat tacaacttta gatgtcacac aaattgcaaa caaaaatgtc
     5941 ttgctctgtc aaaaagaatt ggcaaacgga aatttaaaat atctggcagt atatgatatc
     6001 aatggtggcg actatgcaca tggtgcattt ttgcagtaca ctatttcagg aacagacggc
     6061 accattttgc aaatagatac ttttgcgttt aatttacctt tggcaaatga tgaaaatttc
     6121 agaagagttg aaattgatga tgaagaaaat attcttattc tcgggcagaa atcgtttacc
     6181 aaattagata aaaaaggaaa tcttatctgg caaagaactc cgtttgaaga tattcgttta
     6241 tttgatgtct tgggtacaaa ataccatctc acctcattta ccaccacatc aaacggcaat
     6301 tatttactga caggaaataa tttacaaacc accaatgcaa acagcagtgg tatttatttc
     6361 tttttgatta ctgccgacgg tcaatctaaa gtgaaagcat taacaggaaa ggtttatata
     6421 gacattgaca aaagctgtac ttataccaat aacgaaaccg gttatcccgg attacaagtg
     6481 gttgctgaac gcaacgggca acgtcattat gcctccacag acacatcagg tacttactat
     6541 atgcctttag attcaggcat atacaacatt actgtgcaat tgcccaattc gaatgtatgg
     6601 aagatgtgct atcaaagcta tatggctaac cttactgtaa accagcctct ttcgttctta
     6661 aatattcctc tttatcccaa agtcacatgt ccatttttga atgtagaaat tggcactccc
     6721 tatctgcgca aatgtgctga aaattattat acactatatt attgcaatac cggaaatgat
     6781 actgcacatt ccacttatat agatgttcag ttagatgcag atatgagttt tacccatgct
     6841 gatttatcgt caattaatct gggaaataac caatatcgtt tccaattggg caatctcaac
     6901 ccgcaaaact gtggttcgtt taaaatttat gcacaattaa gttgtgatgc aattgcagga
     6961 aaaacgcatt gtacatcagc gcatatttat cccgataatg cctgcccgcc ggttgcatct
     7021 caatgggacg gctccgatat taaactttat ggatattgcc aacaagacag tgccattatt
     7081 gtgattaaaa acactggaaa caatatgcaa tcacctcgac attatattgt aattgagggc
     7141 gattttttgc gtatacaacc tcaaaattat cagttggcgg ctaacgacag tattgtttta
     7201 aggctaagcg cagatggaca aacaataagg gtttcggcac aacaagatcc caactttcca
     7261 tatcccagcc aacccagtat tacgatagaa agctgctccg gtgtgattga cagcacagga
     7321 ctggttaatc aatttccaca tgacgatgct gcagcattta tcagtacatc ttgtgtgcaa
     7381 aacagaagtt catttgatcc taacgagaaa atggctcagc ctgtaggcta tttgcaagaa
     7441 cattatgtag aaaaaaatac ggaactggaa tacacgctgc gattccagaa tatgggcaac
     7501 gatacggcat tttttgtaga attactggac accctttcaa caggattaga tctgaaaacc
     7561 ttagtgatgg gtgcatcatc acatccatac aactatgcag tatacggcgg tcatattttg
     7621 caaattaatt ttcccggtat taagttaccc gattcattga ccaatgaacc gatgtcacac
     7681 ggatttgtta ctttccatat ccaacccaaa tcaacgattc caaatggaac attgattgag
     7741 aataatgcac aaatttattt tgattataat agcgccgtaa ccacaaacac catttatcac
     7801 accgttgaca gcattcgata taaaatagtg agcttagtaa attatcaccc cacaatcaat
     7861 aaaattgctg tttatcctaa tccgttcagc acttttgctc atattgaaat taactcaagc
     7921 cagccgctta aaaacacaac agtacaagtt tggtcaattg atggcaaatt aatactaaac
     7981 cagcctttag aaaaagatat tgatggtcgt ttgcttacag aaggtatgta tattttaaaa
     8041 attctggccg aaggaaaatt attggcaaca gccaaattga ttaaacaata agcggaatat
     8101 cgcgcagttt tttcttaatt ttacatagat gtgcgatacg tttgtatatc tgcctgaatc
     8161 aatagaagag ccggttattt ttggaaaaaa ctcggacaga gagccggatg aggctcaggg
     8221 catagttcat tatcccaact attcaacatc ctcaaaatct caacgcacaa cctatattga
     8281 ggttgaaacc gatgcgcaaa agctggaagt ttatttatca aaaccatttc agttgtgggg
     8341 ggcagaaatg ggtgtaaatc agtatggcgt agccataggc aatgaggcgg tttttacaaa
     8401 gctgccaatt gaaaaaaaga atacaggatt gacaggcatg gacttattgc gattagcatt
     8461 agaacgagca ataacagcaa aggaagctct ggaaaatatt attttatttt tggaaaaata
     8521 tggtcagaat gcctgtggcg gatataggga taagaagttt tattatcaca attcatttat
     8581 tatagccgat gcacacagtg cctttgttct ggaaacggca ggaacatttt gggtctatca
     8641 aaaaataaag ggattcagag ccatctccaa cggattgagc atagaacaag agtttgatgg
     8701 cattcatccc gatgccattg cacatgcgca taaaaaagga tgggtaaaga aacagcaagc
     8761 tttc
//
LOCUS       DAOQII010000092         8685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4349_length_8685_cov_5.029200, whole genome shotgun sequence.
ACCESSION   DAOQII010000092 DAOQII010000000
VERSION     DAOQII010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4349_length_8685_cov_5.029200"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2135)
                     /locus_tag="PLF48_05875"
     CDS             complement(<1..2135)
                     /locus_tag="PLF48_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNF48889.1"
                     /translation="MNLPDTILQQLAKQIEGELFFDKKMRVLYATDASVYKEYPLAVC
                     YPKNENDIAKLIAFAHQHKTSLIPRTAGTSLGGQVVGNGIVVDVSKYFTDIIEVNKDE
                     HFCIVQPGVIRDELNRFLKPYGLLFGPETSTANRAMVGGMVGNNSCGANSIVYGSTRN
                     HLLELEGFLSNGEKIKINALGDDAYATKLTSSTLEGNIYRFLHEKLSDKNIQKEITTN
                     YPQASIHRRNTGYALDSLLNQKPFNENGNDFNLAELICGSEGTLFFITKMKLNLVPVP
                     PKEKIVVAAHFHSLEESLRATVFAMQHQPTACELMDKIILDCTKANTTYNKYRFFIDG
                     DPAAILCVEFHGETKDAVLQKANALITHFQNEKIGYHFPQLQGNDINKLWSLRKAGLG
                     LLANIPGDRKAVAVIEDTAVTVEDLPAYISEFTDMMNRHQQQSVYYAHAGAGELHLRP
                     ILDLKLKEDRILFRTIAEETADLVKKYKGSLSGEHGDGRVRGEFIPKMIGEKNYELLR
                     QLKYTFDPENIFNPGKIVDTPPMDTSLRYEENQQTRQFDTVFDFSEDEGILRAAEKCN
                     GSGDCRKLPGSGGAMCPSYMATRNEKDTTRARANILRDFLTNSTQANAFDHKEIYEVM
                     DLCLACKGCTSECPSNVDIPTLRAEFLHQYYKSNGIPLRTKAIANIGQLNKLAMLFPA
                     LSNWGLSNKFTSKILKKTLHIAPLRSMPL"
     gene            complement(2253..3458)
                     /locus_tag="PLF48_05880"
     CDS             complement(2253..3458)
                     /locus_tag="PLF48_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNF48890.1"
                     /translation="MNLKNKIQLLADTYFEELIAIRRHLHEHPELSFHEKETMLFVAQ
                     KLSDYGIEHTTNIGGFGVVGLIKGRNPESKTIALRADLDALPIEEKNACSYISKNEGV
                     MHACGHDVHTTCLLCAARILSQLKDEFEGTIKLIFQPAEEKLPGGASIMIKEGVLKNP
                     DVELIFGQHVLPQLQTGKVAFKSGISMASCDEIYITVKGKGGHGAVPNLAVDPVLIAS
                     HIVVALQAIVSRNANPLMPSVLSIGKFIANGATNIIPEEVKMEGTFRTFDEKWRAEAK
                     EKIERTCHHIAECFGGYCEVFIDNGYPYLNNNEQATAQAFELAKEYLGNEQVETMPAR
                     MTAEDFAYYSQEIPSCFFRLGTGNIEKGITSPIHTPTFDVDENCLKTGSGLMAWLAVN
                     ANLRGVSNA"
     gene            3558..4610
                     /locus_tag="PLF48_05885"
     CDS             3558..4610
                     /locus_tag="PLF48_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48891.1"
                     /translation="MKYILQNILITLYISCIADTDKAESNTDANKYIVKYRSDSTIYS
                     DFCQNDSFSYIAHYHRNGKISKERWDTDNFILLSIYNSWDTLGNPLVVNGNGYHYEYY
                     DNGMIKMEGTIKNFLYDSVWRYYTENGEIDYIETYKQINHSTKMIQLKEFGTNGKLRE
                     EFEFNDDGVAGTHRVYDKHTGVLVAIGIFPNTNCRKNYDSLNNNFYSLGFIEVESYFY
                     VDENGILQNTTVFTEGNPNIIFYKCWYNNKQVRIKILYDTLDKTLKYYEYYYNSKLHI
                     IGNIRYFKIDEIAADCSIKFKEDKKSKITAFYGYLKSDIWKEYDELGNLCNEKNMNAE
                     IYEQRKIYFNILDIDE"
     gene            4711..6102
                     /gene="lpdA"
                     /locus_tag="PLF48_05890"
     CDS             4711..6102
                     /gene="lpdA"
                     /locus_tag="PLF48_05890"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNF48892.1"
                     /translation="MNNYDLIVIGSGPGGYVAAIRAAQLGLKTAVVEKENLGGVCLNW
                     GCIPTKALLKSAQVFDYLQHAADYGIESASAHADFSAIVHRSRNVAESMSKGVQFLMK
                     KNKIDVISGYGKVLPGKRVEVTPAEGNIRIIKAENIILATGSRARQVPSLPIDGKYII
                     DYRKALTLPQQPENMVIVGAGAIGVEFAYFYQSLGTKVTLIEFFDTLLPNEDKEIGKT
                     LEKIFTKKGMTIKTSARVISSKIEGNKVHLFVNGQDGEQNFIADVVLSAVGIKSNLEN
                     IGLELCGIEMENDKIKINKFYQTNVEGIYAIGDIVKGPALAHVASAEGIIAVEHIAGH
                     HPQPLRYDNIPSCTYCSPEIASVGITEQQAKERGMDILVGKFPFSASGKAKAGGKTDG
                     FVKVIVDKKYGEILGAHMIGENVTEMIAELVVARNIEATGKVILKSVHPHPTMSEAIM
                     EAFAAAYGEVIHL"
     gene            complement(6097..6744)
                     /locus_tag="PLF48_05895"
     CDS             complement(6097..6744)
                     /locus_tag="PLF48_05895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF48893.1"
                     /translation="MKKSVKWKIAQQLEYKWWSRYLEDKNPEAYLKWKLSYWQDLLKS
                     VEEVIPLPEQKTVLDAGCGPAGIFMALHNNDVTAIDPLLNKYKEFIHFDETKYPFVTF
                     QQCTIEELNEKEKFDYIFCLNAINHVNNLSLSYDKLVNSLKPGGYLVISTDAHRSSFL
                     KKIFQWLPGDMLHPHQYSMEEYDAFLTNRCMKVIENIRYKQELIFDYIITIAQKL"
     gene            6858..>8685
                     /locus_tag="PLF48_05900"
     CDS             6858..>8685
                     /locus_tag="PLF48_05900"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNF48894.1"
                     /translation="MSILHNISGLVLKPVIKQMSQTRLPKIDGSVQVKGIEQDVEIIR
                     DEWGVAHIFAQNMYDGLFAQGFAHAQDRLFQMEINRRAARGKLSEIIGKDALDTDRVS
                     RTFGFERVAMQDWDFFSKEEQQIIHAYCAGVNAYIQSDDFQLPIEFKLLKIEPELWEP
                     MDVATFSRLMISLLSWGWYDEIIRAKLIDMVGAEAAAELNNTYPKENPITLPKGIEFN
                     HVDISGKFHALSGPYMPSINGSNAWTISGYKTKTGMPYLCNDPHLALRNPNIWYLNHL
                     DCNDLKVSGVTCPGLPLVQIGHNEAIGWGITLAFTDIESVFIEKFTDDTCTTYMHEGK
                     IKETHILEEKIFIKNTDLPHIEKIYNTVHGTVISDIINGSSAKLTLCSMALQPGKALL
                     GWSLLNKAKNWNDFVDGVKCITAPGLNIVYADTSGNIGYYNSGKVPLRTKEEALIPQE
                     GWTGEHDWKDYVPFEQMPHVLNPEKGYVITANHKVEPDDYPYFLGDIYMNGYRANRLE
                     KMLNSHTKTEPKDYVAMQMDFYCTPSEKFKAYYSGIEFDSAELNNYKDMLLQWDGVLN
                     ADSLPASFYKVTKWMLIKKILESAITDKTLIDELMGKGSHGQI"
BASE COUNT         2808 a         1568 c         1631 g         2678 t
ORIGIN      
        1 tacaatggca tagaacgaag gggtgcgatg tgcagcgttt ttttcagaat ctttgatgta
       61 aatttattgg acaaacccca gttgctcaat gccggaaaaa gcatagctaa tttattcagc
      121 tgtccgatat tggcaatagc tttcgtgcgc agtggaatgc cattagattt ataatattga
      181 tgcaaaaatt cggcacgcaa agtcgggata tctacattgg aaggacactc ggatgtacag
      241 cctttacaag ccaggcacaa atccatgacc tcataaattt ctttatggtc aaatgcattg
      301 gcttgggtag agttggttaa aaaatcgcgc aaaatattgg ctctggctct cgttgtatct
      361 ttttcatttc tggtggccat atacgaagga cacattgcac ctccgctacc cggcaatttt
      421 ctgcaatctc cactgccatt gcatttttca gcagcccgta atatgccttc atcttccgaa
      481 aaatcaaata ccgtatcaaa ttgtcgggtt tgttggtttt cttcatagcg aagtgatgta
      541 tccatgggtg gcgtatctac aattttgccc ggattgaaaa tattttcagg atcaaaagtg
      601 tatttcaatt ggcgcaagag ttcgtaattt ttttcgccta tcattttcgg aataaactcg
      661 ccgcgaacac gtccgtcgcc atgttctccg cttaatgagc ccttgtattt cttgactaaa
      721 tctgcagttt cttccgcaat cgttctgaat aaaattctgt cttcttttaa tttcaaatcc
      781 agaataggac gcaaatgtaa ttcaccagct cctgcatgtg cataatacac ggattgctgc
      841 tgatggcgat tcatcatgtc agtaaattca gaaatatatg ccggcaaatc ttcaacagtt
      901 actgcggtat cttcaataac ggctaccgct tttctgtcgc ctggaatatt ggctaataaa
      961 cctaaaccgg cttttcgcaa actccacaat ttattgatat catttccttg cagttgcgga
     1021 aagtgatagc ctattttttc gttttgaaaa tgtgtgatta acgcattcgc tttttgtaaa
     1081 accgcatctt tagtttctcc gtgaaattca acacataaaa ttgccgcagg gtcgccatct
     1141 ataaaaaaac ggtatttgtt gtatgtagtg tttgctttgg tacaatccag aataatttta
     1201 tccatcagtt cacaagcagt aggctgatgt tgcatggcaa atactgtagc ccgtaaactt
     1261 tcttccaacg agtgaaaatg tgcggcaaca acaatttttt ctttaggcgg caccggtacc
     1321 agattcaatt tcattttggt tataaaaaac aaagtacctt cactgccaca aatcagttct
     1381 gcaagattaa aatcatttcc attttcattg aaaggttttt ggtttaatag agagtcgagg
     1441 gcgtatccag tgtttcttct gtgaatggaa gcttgagggt aatttgttgt tatttccttt
     1501 tgtatatttt tgtctgataa tttttcatgt aaaaagcgat agatattacc ttcaagtgtt
     1561 gatgaagtta attttgttgc ataagcatcg tcacccagtg cattaatttt tatcttttca
     1621 ccgttggata aaaatccttc aagttccagt aagtgatttc tggtagaacc gtaaactata
     1681 gaattagcac cgcaagaatt attgcccacc ataccaccaa ccatagcacg gttggcagtt
     1741 gatgtttcag gaccaaaaag taatccgtat ggtttcagaa atcggttaag ttcatccctg
     1801 ataacacccg gctgaactat acaaaaatgt tcatccttat taacttcaat aatatccgta
     1861 aaatattttg aaacatctac cactatgcca ttgcctacaa cttgcccgcc caatgatgtt
     1921 cctgcagttc gaggtataag agaagtttta tgttgatgag caaaagcaat taatttagca
     1981 atatcatttt catttttggg ataacaaacg gccaacggat attctttata cacagatgca
     2041 tctgtggcgt ataacacacg catcttttta tcgaaaaaca gttcaccttc tatttgctta
     2101 gctaactgtt gtaaaatggt atcaggaaga ttcattactg taaaattaca atgtagagcc
     2161 aactaaaact gaaagcatgt aagaagtaaa agttaatttt tgacctcgta ggtcgaaaat
     2221 acaaaccact aattttcaat aagatatatc tttcaggcat tgcttacacc tcgtaggttg
     2281 gcattgactg caagccaagc catcagtccg cttccggttt tcaggcaatt ttcatccaca
     2341 tcaaaagtgg gagtatgtat gggagaagtg ataccttttt caatatttcc cgttcccaac
     2401 ctgaaaaaac aagatggaat ttcttgggaa taataggcaa aatcttcggc tgtcatacgt
     2461 gcaggcattg tttccacttg ttcatttcca agatattctt tagcgagctc aaaagcttga
     2521 gcagtcgcct gttcattatt attcaaatac ggatacccat tgtcgataaa tacctcgcaa
     2581 tagcctccaa aacactcagc aatatgatgg caggtgcgtt caattttttc ttttgcttcg
     2641 gctctccatt tttcgtcgaa cgtacggaaa gtgccttcca tttttacttc ttcaggtata
     2701 atattcgttg caccattggc aataaactta ccaatactca gtacagatgg catcaacgga
     2761 ttagcatttc tactgacaat ggcttgcaga gctactacta tatgtgatgc aattaacacg
     2821 gggtcaaccg ccagattagg gacagcacca tgtccacctt ttcctttcac ggtgatgtaa
     2881 atttcatcgc agcttgccat agaaattccg gatttgaatg ccactttccc tgtttgtaat
     2941 tgtggcaata catgttgccc aaatatcagc tctacatccg gatttttgag tacaccttct
     3001 ttaatcatga tagacgcacc gccgggcaat ttttcttcag ccggctgaaa aataagctta
     3061 atcgtacctt caaattcatc tttcagttgt gataaaatcc gtgcagcaca gagcaaacaa
     3121 gtagtatgta catcgtgacc gcaagcatgc atcacacctt cattttttga tatatagcta
     3181 caagcatttt tttcttcgat gggcaatgca tccaaatcag cacgcaaggc aatcgttttg
     3241 ctttcaggat ttcttccttt tattagtcct acaactccga agccgccaat atttgttgta
     3301 tgttctatac cataatcact taacttttga gctacaaaaa gcatggtttc tttttcgtga
     3361 aaactcaatt ctggatgctc atgcagatgc ctgcgtatgg caatgagttc ttcaaaataa
     3421 gtatctgcta ataactgtat tttatttttt aagttcatag aattatgtaa agatataaaa
     3481 tacgggtaaa aattgctgct ataagctagc aactctaaaa taatattgct aacttttcgt
     3541 tacatttatt tgcgaacatg aaatatatac ttcaaaacat tctaattaca ctttatattt
     3601 cttgtattgc agacacagat aaagctgagt caaatacaga tgcaaataaa tatattgtta
     3661 aatacagaag tgatagtaca atatacagcg acttctgtca aaatgattca ttctcctata
     3721 tagcacatta tcaccgtaat ggaaaaatct ctaaggaaag atgggataca gataatttta
     3781 ttttactttc tatatacaat tcttgggata cacttggtaa tccattagtt gtaaatggca
     3841 atggatatca ctatgaatac tatgataatg gtatgataaa gatggaggga acaattaaga
     3901 attttcttta tgatagtgta tggagatact atactgaaaa tggagagatt gactatattg
     3961 aaacgtacaa acaaatcaat cattcaacta agatgattca actaaaagaa tttggaacaa
     4021 atggcaaatt aagagaagaa tttgaattta acgatgatgg agtagctggc actcacagag
     4081 tttatgataa acatacagga gttttagtgg caataggaat attcccaaat actaattgta
     4141 gaaaaaatta tgattcttta aacaataact tttatagctt aggttttata gaggttgaat
     4201 cttattttta tgtggatgag aatggaattt tacaaaacac tacagtattt actgaaggaa
     4261 atccaaatat tatattttac aagtgttggt acaacaataa acaagtcaga attaagattc
     4321 tatatgatac tttagataaa acattaaaat actatgaata ttattataat tcaaaattac
     4381 atattattgg caatatcagg tattttaaaa ttgatgaaat agccgctgat tgttcaataa
     4441 aattcaaaga agataagaaa tcaaaaataa cagcgtttta tgggtacttg aaatctgata
     4501 tctggaaaga atatgacgaa ctgggtaatc tgtgtaatga aaaaaacatg aatgctgaga
     4561 tttatgaaca acgcaaaata tattttaaca ttttagatat tgatgaataa ctttcttgtt
     4621 ataaaacaaa ctctattttc tatatgattt aaagctaact actaaaaatt atttccttaa
     4681 ttttatgcag tatcattagc aagtaaatgt atgaacaact atgatttaat agtaatcggc
     4741 tccggtcccg gaggttatgt tgccgctatt cgggcagctc agttaggttt gaaaaccgct
     4801 gtagtagaaa aagaaaattt aggtggagtt tgcctcaact ggggttgtat tcctactaaa
     4861 gctttgctaa aaagtgcgca ggtgtttgat tatttacaac atgcagctga ttacggcatc
     4921 gaatcggcat cggcacatgc cgacttttct gcaatcgttc accgcagcag aaatgtagcc
     4981 gaaagcatga gcaaaggagt ccagtttcta atgaaaaaaa ataagattga cgtaatttcc
     5041 ggttatggaa aagttcttcc gggaaaacga gtggaagtaa ctcccgctga aggaaatatc
     5101 agaattatta aagcagaaaa tatcatatta gcaacgggta gcagagccag acaagtaccc
     5161 tctttgccca tagacggcaa atatatcatc gactatcgca aagcattgac attacctcag
     5221 caacctgaaa atatggtcat cgtaggtgcg ggtgccattg gagtagagtt cgcttatttt
     5281 tatcagtcat taggaacaaa agttaccttg attgaatttt ttgacacgct actgccaaat
     5341 gaggataagg aaatcggcaa aactttggag aaaatattta cgaaaaaagg catgacgatt
     5401 aaaacatcag ccagggtaat atcttccaaa attgaaggaa ataaagttca tctctttgta
     5461 aacggacaag atggagaaca aaatttcatt gccgatgtag tactcagtgc agttggaata
     5521 aaatctaatt tggaaaatat cggattggaa ttatgcggaa ttgaaatgga aaacgacaag
     5581 ataaaaatca ataagtttta tcaaacgaat gtggaaggaa tttatgcaat cggtgatatt
     5641 gtaaaagggc ctgccttggc acatgtggcc agtgccgaag gaattatagc tgttgagcat
     5701 attgcagggc atcatcctca acccttgcgt tatgataata taccatcttg tacttactgt
     5761 tctcctgaaa tcgcaagtgt gggaattacg gaacaacaag ctaaagaaag gggcatggac
     5821 attttggttg gaaaatttcc attttcagcc agtggtaaag caaaagcagg tggaaaaaca
     5881 gatggctttg taaaagtgat agtagataaa aaatacggtg aaatattggg agctcatatg
     5941 attggtgaaa atgtaacgga aatgatagca gagctggtag ttgccagaaa tattgaagct
     6001 accgggaaag tgattttaaa atccgtgcat cctcacccta ctatgagcga agccattatg
     6061 gaagcatttg ctgcagctta tggcgaagtg atacatttat aatttttgag caatagtaat
     6121 tatatagtca aaaatcagct cttgcttata gcgtatgttt tcaattactt tcatgcatcg
     6181 attagtcaaa aatgcatcgt attcctccat gctgtattga tgcggatgta acatatctcc
     6241 cggaagccat tggaatattt tttttagaaa cgaggaacga tgtgcatccg tagatattac
     6301 taaatatcct ccgggcttca atgaattaac taatttatca taactaagcg ataaattatt
     6361 gacgtgatta atggcattta gacaaaagat ataatcaaat ttttctttct cattaagttc
     6421 ttcgatcgta cattgttgaa atgtaacaaa aggatatttc gtttcgtcga aatgaatgaa
     6481 ttctttgtat ttattcagca aagggtctat agcagtaaca tcgttattat gaagtgccat
     6541 aaaaataccc gcaggaccgc aacctgcatc taaaacagtt ttttgctctg gcaagggaat
     6601 aacttcttct acagatttta gcaaatcttg ccaataactc aatttccact ttaaataggc
     6661 ttccgggttc ttatcttcca gatatcggct ccaccattta tactccaatt gctgtgcaat
     6721 tttccacttc accgattttt tcattaacta aagatagaaa attctgctat acggaagttc
     6781 taatgcatat ctatttgtgc attctgtttc catatcacca tttaagtgta tctttatcgt
     6841 ttaattttgt aaatcacatg tctattcttc ataatataag tggtcttgtt ttaaaacctg
     6901 ttatcaaaca aatgagccaa acacgcctgc caaagattga cggcagtgta caagtcaaag
     6961 gtatagaaca agatgttgaa attattcgtg atgaatgggg cgtagcacac atttttgctc
     7021 aaaatatgta cgacggatta tttgcacaag gctttgccca cgcacaagat cgtttgtttc
     7081 aaatggaaat aaaccgaaga gcagcacgtg gtaaactaag tgaaattatc ggcaaagatg
     7141 ctttagatac cgatcgtgtt tcgcgtactt ttggcttcga gcgtgtagcc atgcaagatt
     7201 gggatttttt ttccaaagaa gaacagcaga ttatacatgc atactgtgca ggagtaaatg
     7261 cctatatcca gtctgacgat tttcagttac ccatcgaatt taagttatta aaaatagagc
     7321 cagaactgtg ggagcctatg gatgtggcga cattcagccg gcttatgatt tcgcttttaa
     7381 gctggggttg gtatgatgaa attatcagag ccaagcttat agatatggta ggcgcagaag
     7441 cggcagctga actcaataat acttacccaa aagaaaatcc gataacacta ccaaaaggca
     7501 tagaatttaa tcacgtcgat atttcaggta agtttcatgc cttaagcggg ccttacatgc
     7561 ccagcataaa cggtagtaat gcgtggacaa tttccggcta taaaaccaaa accggcatgc
     7621 cctacttatg taacgatcct catctggcac tgcgcaaccc aaatatctgg tatctcaatc
     7681 atctggattg taatgattta aaagtaagcg gtgttacctg ccccggattg ccattggtac
     7741 aaatcggaca taatgaggct atcgggtggg gaataacgct tgcatttaca gatatcgaaa
     7801 gtgtatttat cgaaaaattt actgatgata cctgcacaac ttacatgcat gaaggcaaga
     7861 taaaagaaac acacatttta gaagaaaaaa tatttataaa aaacaccgat ttgccccata
     7921 tcgagaaaat atacaatact gtgcacggca cagtgatatc agatattatt aatggcagtt
     7981 cagcaaaact taccttgtgt tctatggctt tgcagccggg taaagccctg ctgggatggt
     8041 ctttattaaa taaagcaaaa aactggaatg attttgtaga cggagtaaaa tgcattactg
     8101 ctcccgggct taatattgtt tatgcagata cttcaggcaa tattggctac tataattcag
     8161 gtaaagtgcc tttaagaacg aaagaggagg cattgattcc acaagaaggc tggacaggtg
     8221 aacacgattg gaaggactat gtcccttttg agcaaatgcc gcatgtattg aatccggaaa
     8281 aaggctatgt catcactgct aatcacaaag tagaacccga tgattatcct tattttttag
     8341 gcgatattta tatgaatggt tatcgggcta atcgacttga aaaaatgctg aacagtcata
     8401 ccaaaacaga gcccaaagac tatgtggcta tgcaaatgga cttttactgt acaccatctg
     8461 aaaaattcaa agcttactac tccggtattg aatttgattc tgctgaattg aataattata
     8521 aagacatgtt gctgcaatgg gatggtgtat taaatgcaga cagcttgccc gcaagttttt
     8581 ataaagtaac taaatggatg ctcattaaaa aaattctgga atctgccatt acagataaaa
     8641 cactcattga tgaattaatg ggaaagggtt cacacggaca aatag
//
LOCUS       DAOQII010000093         8479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4506_length_8479_cov_6.277066, whole genome shotgun sequence.
ACCESSION   DAOQII010000093 DAOQII010000000
VERSION     DAOQII010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4506_length_8479_cov_6.277066"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..424
                     /locus_tag="PLF48_05905"
     CDS             230..424
                     /locus_tag="PLF48_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNF48895.1"
                     /translation="MKNTSISLGNYFDQFVSSQVSAGRFKNVSEVIRAGLRLLEDEEI
                     KLIALRNAIQEEIDSGIATT"
     gene            complement(707..2578)
                     /locus_tag="PLF48_05910"
     CDS             complement(707..2578)
                     /locus_tag="PLF48_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48896.1"
                     /translation="MYKWGLFLCLGLIFRSAVAQIEITKPNPVLYYNTKYLTLEKPIL
                     RNPDTSYREFHRYNIIENQDLPYNNLGNFGTAYYSLMFSNVNPIGYKHGFNSFDIYYN
                     KPEKIKYYRTKTPYTFLDFIFGGKEEIIGGGEFSYNIKPNYNFAFNFHRNNFKGKSAH
                     QLAAHNLLTFQQWFVSRNNFYDLKFNFSFNGIKVEENGGWAVDDAYTNSLYKKHKDIV
                     PVFMEDAYNKWNEKNINFVQQFNLGNKEEITINDSTKRKIIKPKYTIEHDFEFSHNKF
                     LYKDYEDDSAYYENWYYNSDSTTDLTKSYTVSNGIYFKNYQNDTQPRKFLYEAGLQYA
                     FNHYQHMNAVQNFSDIQLKARLYSLNDSAFFNYSVSASACVLPPYIGDFEVNFSGKIN
                     LNKKHVLALSGNISTAEPTQKQAFYLGNHVRYNHDFKKVFQVKIQADYAWEKQLLFAS
                     IQNYFIQNYIYFDTSSITQQYHQPLNVLIAKIRKDFNTKHIYTGTEIYAQWISNRNLV
                     RLPVFYIKQTVYYKGGFISGKLNAQLGFDITYNTLFKGNAYNPSLAIFYQQDKTTLKF
                     YPVLDLFFNLHVKRTNIFLRMLHVNQGMFKQKGLYTAPNYGYFDRTFRVGVTWQFYD"
     gene            2777..4270
                     /locus_tag="PLF48_05915"
     CDS             2777..4270
                     /locus_tag="PLF48_05915"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF48897.1"
                     /translation="MAKNELSVLITGTGFSGICVGIQLKKMGFHNFTIVEKSNKPGGT
                     WRDNDYPGAACDVKSNLYSYSFEPNPHWSRQFSPQAEILAYTVHCIHKYGLDEHIVYN
                     WEAKSAKYDETNATWEITNQNKKKMKANIVIFGNGPLHIPSLPDIEGIKDFKGEIFHS
                     SRWNHDYDLRDKKVCVIGTGASAIQFVPEIQPLVKNLYLMQRTAPWVLPKPDGPFSPT
                     QSVLFEKLPMIQNLDRNFIYYLNEGQAVGMIHNDAILKLGEVLGKRHIKKFIKDPVLQ
                     KKLTPTYRLGCKRVLMSNNYYQALSKPNVDVVTDGILKFTKDSVITKDGKERKIDAVI
                     MGTGFHVADSFQYFDVTGKDGAEMNKIFKDSPEAYYGSTVNKFPNMFIMLGPNTGLGH
                     NSMIYMIESQVNYIVDAIKKMQKNDIKSFEVKKDVQTEFNQKIQKKLEGTIWMSGCKS
                     WYLSADGKNYTVWPDFTWKFRDQTKHFNLKDYSLVKEDIAVAEPELV"
     gene            4383..5807
                     /locus_tag="PLF48_05920"
     CDS             4383..5807
                     /locus_tag="PLF48_05920"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNF48898.1"
                     /translation="MKNIILAGLFFAFLSLQAQEQTTYLKVRKINFSNNKKTRPYVML
                     REMSFNEGDSIPGDKLDSILLANRLQIFNLGIFNDIQVNIKNWEEDSLDLIIKVYEKF
                     PLSPGIILQFADRNTAEWWRKYNHDFKRLQYGLILNYNNVSGRNDKLIGAISFGFAKR
                     LDLGYIIPAFDSKKDNVGMALFFNFLHSKRIAYNTLDNELKYMDITAKAQLIKTEFTA
                     SINYRRELHNTHYFSVGYGFSKVSDYVLLANPEYFLDGVRKQNYLKLGYVFESDHRNL
                     KAYPTNGWYIRMNFTNYGLGFMKTRMTTAGFMMSRYYEWKKHKKFSLGFSGRFQVSWP
                     FKQPYNLQPIKSFGYNDNLIRGYEVNVMDGQHFLLFKNEYRYRLFSFQLKNLKKIKNK
                     NNKFLNSSLAFLPFNMYLTAYFDAGYVWDNAHYAQNSYRNKWQFGYGVGLNLVTFNQR
                     LFRIEYSINRYLDKGVYLHFELPF"
     gene            5807..6685
                     /locus_tag="PLF48_05925"
     CDS             5807..6685
                     /locus_tag="PLF48_05925"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNF48899.1"
                     /translation="MAIGIYSRVFKEEHAPFVLQLIRALQDQQIDFCIEDNYFKQIKP
                     ILPARKYALFNNHIDIKKKTDLLIALGGDGTLLDTIQFVRDSEIPVAGINMGRLGFLA
                     DIHKQEIEELVTSIKSNTYTIEDRALICAELNKPVFGEVNYGMNEFTIHKTDSSSMIV
                     IHTFLNGEFLNSFWADGLIIATPTGSTAYSLSCGGPIVFPNSNNFVITPVAPHNLNAR
                     PMIIPDNTVLSFEVECRSKTYLCSIDSRSKSLDDTFQVAVRKADFYFKLLRMPESSYL
                     KTLREKLNWGEDTRNS"
     gene            complement(6710..7417)
                     /locus_tag="PLF48_05930"
     CDS             complement(6710..7417)
                     /locus_tag="PLF48_05930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF48900.1"
                     /translation="MYCRQSNNVLRQTKIKKIFNDLAEDYWLNNIFSFGLTAYIRKQA
                     IDKLKIKQNESVLDIMCGTGDNFKYLFRFTNHITALDYSENMLSVARASNKYSKSVHL
                     VKKDFLSDDISELKFNNCLCTFGLKQLQDDEIPIFVHQLRTVLCAQGRFCMAEICLDN
                     RSFSSRMALNYMHYFLPIINKLITGKNHHKYLYSFAKNTVPVQYIIQILQENNFEVDT
                     DFIAFKHAVVISGKYLG"
     gene            complement(7377..8354)
                     /locus_tag="PLF48_05935"
     CDS             complement(7377..8354)
                     /locus_tag="PLF48_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48901.1"
                     /translation="MKTFDDAVLDDNNPIVSISLMGKIVFIAILSVFVSMCLVILYDT
                     EYGFMLFIGVPLSIGFTAGFIMKNPTKSIFHIFNKVLLIFIVITLLLILCKVEGGICI
                     IMIAVPLYILLLFSYALGHIIRVHYNITQKPFLFSLILINPFFAGIDLSMKDGFTQHI
                     EDKLTINADTSIVWNVLTQPVQYAEPTNFFFKYGVNYPKNMQVTAANDSLYLVCNLRN
                     GDAALKITSLKEDSLLRFEPRQAILPIKELTVYKNINTPHSHDAYFKINYGEFMIKQL
                     GENKTELYATTELTHHLRPYFYWNLWNRYIIHQMHKNVLQTIKQRVEAN"
BASE COUNT         2784 a         1452 c         1604 g         2639 t
ORIGIN      
        1 ttgtaatttt aaatactatt tgtttttagg ctttagtttt ctgttgcata ctgtgagtag
       61 tacaggcgat aaactaaaaa aaataaggct gtttagggct gtctcgtcct gaaataggtt
      121 tacacattta taactcaaaa aactaatcga aaaacaaaca cgaaaatttc gagacaagaa
      181 cacttgtata attaccaaat tttggtaacc ttgaataaag tttataaaaa tgaaaaatac
      241 atcaatttca cttggaaatt attttgacca gtttgtgagt agccaagtat cagcaggacg
      301 ctttaagaat gtaagtgaag ttattcgagc cggacttcga ctgttggaag atgaagaaat
      361 taaattaatt gctttgagaa atgccattca agaagaaatt gacagtggaa ttgccacgac
      421 ttagacccta aaaaacatct tcaagaatta aaggcgaaac gaagaaacaa tggctaaata
      481 taaattgacg aataaaagct attgaggatt tgacacaaat ttgggattat acctttgaca
      541 aatgatctga aaacaatcgg acaaatatta ttaacctgag ttcgggataa gcgtttcaat
      601 taaaaaatag ttgattctca cgatatcgtt ccgccttaaa aggtggtggt ttataccata
      661 aaatcaactt tttttcaagt aaaaagcaaa ttcagaaatc tgcttattaa tcataaaact
      721 gccacgttac accaacacgg aaggtgcggt cgaaatatcc ataattgggt gcagtataaa
      781 gtcctttttg cttaaacata ccctggttga catgcagcat acgcaagaag atattggtgc
      841 gctttacatg cagattgaaa aacaaatcca aaaccggata aaacttcagg gttgttttat
      901 cttgttggta aaagatagcc aaagacggat tataggcatt tcctttaaat agcgtgttgt
      961 aagtaatatc aaaacccaat tgagcgttca acttcccgga gataaagccg cctttgtaat
     1021 acacggtttg tttgatgtaa aagactggca agcgaaccaa attccggtta gaaatccact
     1081 gagcatatat ttcagtaccc gtataaatgt gtttcgtgtt aaagtctttc ctgattttgg
     1141 caatcaacac attcaacggt tggtggtatt gttgggtaat ggatgacgta tcgaaataaa
     1201 tataattttg tataaagtaa ttttgtatgg aagcaaaaag caattgcttt tcccatgcat
     1261 aatccgcctg aatttttact tgaaaaactt tcttaaaatc atgattatac ctaacgtgat
     1321 tacctagata aaatgcttgt ttttgtgtcg gctctgccgt cgagatattg ccgcttagtg
     1381 ccaggacgtg ttttttatta aggttgattt ttcccgagaa atttacttca aaatctccga
     1441 tatatggagg taaaacgcac gcagaggcag acaccgagta gttgaaaaat gcactatcat
     1501 tgaggctata gagtctggct tttaattgaa tgtcgctgaa gttttgaaca gcattcatgt
     1561 gttggtaatg gttgaaggca tattgcaatc cggcttcata taaaaacttc cggggttgcg
     1621 tgtcgttttg atagttttta aaataaatgc cattactcac ggtatatgat ttcgttaagt
     1681 ctgtcgtaga atcgctgttg taataccagt tttcatagta agcggagtcg tcttcatagt
     1741 ctttatacag aaacttattg tgcgaaaact cgaaatcatg ctctatggta tattttggtt
     1801 taataatctt gcgtttcgtg ctgtcgttta tagtgatttc ttctttattg cccaaattaa
     1861 actgttgtac gaaattgatg tttttttcgt tccatttatt gtaagcatct tccataaaaa
     1921 caggcacaat gtctttatgt tttttataca agctattggt atacgcatcg tctaccgccc
     1981 aaccgccatt ttcttctact tttatgccgt taaaactgaa attgaatttc aagtcataaa
     2041 aattatttct agacacaaac cattgctgaa aagtcagcag attgtgcgca gccagctggt
     2101 gtgcactttt ccctttgaaa ttattacggt gaaaattgaa tgcgaaatta taattaggct
     2161 taatgttgta ggaaaattca cctccgccga taatttcttc cttaccgccg aagataaaat
     2221 ccaaaaaggt atagggtgtt ttggtgcggt aatattttat tttttcaggt ttgttataat
     2281 aaatatcaaa actgttaaaa ccatgtttat atccaatcgg gtttacgttt gaaaacatca
     2341 atgaataata ggcggtacca aaatttccca ggttgttata tggcaaatcc tgattttcga
     2401 taatattata gcgatgaaac tcgcggtatg acgtgtcggg attgcggagt atgggttttt
     2461 ctaaggtaag gtattttgta ttgtaataga gtaccggatt gggctttgta atttcaatct
     2521 gagcaacagc cgacctgaat atcaatccta agcatagaaa aagaccccat ttatacacaa
     2581 ggcaaaaata caattttaaa ctaatggaat aaacaactta aaaagacgtt aatctaccct
     2641 gaattatgct gatagcaaaa ggatttttca ttccgctctt acgctttaca taagttaact
     2701 tattttcatc ttaaccttgg ataagcaaga ataaaaaccg tagcccttaa atttatatcc
     2761 taaatatttt ttgggtatgg caaaaaacga actttcagta ttgattaccg gcaccggatt
     2821 ctccggcatc tgtgtgggca tacagcttaa aaaaatggga tttcacaatt ttactattgt
     2881 agaaaagagc aataaaccgg gtggcacatg gcgagataat gattacccgg gagcggcttg
     2941 tgatgtaaaa tcaaatttgt attcctattc ttttgagccc aacccccatt ggagccggca
     3001 gttttcgcct caggctgaaa tattagccta taccgtacat tgcatacaca aatacggact
     3061 cgatgaacat atagtgtata actgggaagc aaaatcggca aaatacgatg aaactaatgc
     3121 cacttgggaa attaccaacc agaacaagaa gaaaatgaag gcgaacattg tcattttcgg
     3181 caatggtccg ttgcatatac cgtcccttcc agatattgaa ggtataaaag atttcaaagg
     3241 agaaattttt cattcctccc gctggaatca tgattatgac ctcagagaca aaaaggtatg
     3301 tgtaattggc acgggagcca gtgccattca gtttgtaccc gaaattcaac cccttgtaaa
     3361 aaatctctat ttgatgcaac gtacagcacc atgggtattg ccaaagcctg acggtccatt
     3421 ttcccctaca caaagtgtct tgtttgaaaa attacccatg attcaaaatt tagaccgcaa
     3481 ttttatctat tatttgaatg aaggacaggc ggtaggcatg atacataatg atgctattct
     3541 aaaactggga gaagtgttgg gaaaacgcca tatcaaaaaa tttatcaaag atcccgtgtt
     3601 gcagaaaaaa ttgacaccga cctatcgttt gggatgtaag cgtgtattaa tgtcaaataa
     3661 ttactatcag gctttgtcaa aaccgaatgt ggatgtagta actgatggta ttttaaagtt
     3721 tacgaaagac agtgtgatta cgaaagacgg taaagaacga aaaattgatg ccgttatcat
     3781 gggtacaggg tttcatgttg ccgacagttt ccaatacttt gatgtaaccg gaaaagacgg
     3841 ggcagaaatg aacaaaatat tcaaagactc gccggaagca tattatggca gcacggtcaa
     3901 caaatttcca aatatgttta tcatgctcgg cccaaatacg ggattgggac acaactctat
     3961 gatatacatg attgaatcac aggtgaacta tattgtcgat gctatcaaaa aaatgcagaa
     4021 aaacgatatt aaatcgttcg aagtgaaaaa ggatgtacaa acggagttta atcaaaaaat
     4081 tcaaaagaaa ttagaaggca ctatatggat gtcgggatgt aaaagctggt atctgagtgc
     4141 agatggcaaa aactataccg tctggcctga tttcacctgg aaattcaggg atcaaacgaa
     4201 gcattttaac ctgaaagatt attccttagt aaaagaagac atagccgtag cagaaccgga
     4261 attggtatag gttttatcat tctctgtttg atcatatgat atgcgtagtg ttgtacaagt
     4321 taagtttact taactgttca gctaaaccta taaaataaac tacctttgta ggtttttagc
     4381 ggatgaaaaa catcatattg gctggtttgt tttttgcttt tttgagttta caggctcaag
     4441 aacaaacaac gtatttgaaa gtcagaaaga ttaatttttc caacaacaaa aaaacacgcc
     4501 cctatgtaat gttgcgggaa atgtctttta acgaaggcga ttcgataccg ggagataaat
     4561 tagacagtat tttgctggca aaccgactgc agatttttaa tctgggaatt tttaatgaca
     4621 tacaggtcaa tattaagaac tgggaagaag acagtttgga cttaatcata aaagtatatg
     4681 agaaatttcc gctaagtccc ggtataatct tacaatttgc cgacagaaat acagcagaat
     4741 ggtggcgcaa atacaaccat gatttcaagc gcctgcaata tggtttgatt ttaaactaca
     4801 acaatgtaag tggtcgcaat gataaactca tcggcgcaat tagttttggt tttgccaaac
     4861 gcctcgattt gggttatatc atcccggcat ttgacagcaa gaaggataat gtggggatgg
     4921 ctttgttttt taatttttta cacagtaaac gcatagccta taatacatta gacaacgagt
     4981 tgaagtatat ggatataacg gcgaaagcgc agctgataaa aacagaattt acggcaagta
     5041 taaactatag gagagaactg cataatacgc attatttttc ggtaggctat ggcttttcaa
     5101 aagtaagtga ttacgttttg ttggctaatc ctgaatattt tctggatggc gtccgtaaac
     5161 agaactacct taaacttggc tatgtattcg agtctgatca ccgcaatttg aaagcctacc
     5221 cgactaacgg atggtatatc cgcatgaatt ttaccaatta cggattgggt tttatgaaaa
     5281 cacgtatgac tactgccgga tttatgatga gcagatatta cgaatggaaa aaacacaaga
     5341 aattttcatt gggcttttcg ggcaggtttc aggtatcctg gccctttaag caaccctata
     5401 atcttcaacc cataaagtca ttcggatata atgacaacct tatccgcggg tatgaagtaa
     5461 atgtaatgga cggacaacat tttctgttgt ttaaaaatga ataccgctac cggcttttca
     5521 gttttcaatt aaaaaatctc aaaaaaatca agaacaagaa caataaattc ttaaactcct
     5581 ctttggcatt tcttcctttt aatatgtatc tcaccgccta ttttgatgcc ggttatgttt
     5641 gggataatgc ccattatgcc caaaacagtt acagaaacaa gtggcaattc ggctatggtg
     5701 tgggtttaaa tttagtgacg tttaaccagc gtttgtttag aattgaatac agcatcaaca
     5761 gatatttgga taaaggtgta tatttacatt tcgagttacc tttctgatgg caatcggtat
     5821 ttacagcaga gtttttaaag aggagcacgc tcctttcgtt ttgcagctta ttcgtgcact
     5881 gcaagaccaa cagattgatt tctgcataga agataattat ttcaaacaaa taaagccgat
     5941 acttcctgcg agaaaatatg ccttattcaa taatcatatt gatataaaaa agaagacaga
     6001 cttattaatt gctttgggcg gagatggcac cttattggat accatacagt ttgttcgcga
     6061 cagtgaaatc ccggtagcgg gtattaatat gggcaggtta ggttttctgg ctgatattca
     6121 caaacaggaa attgaggagt tggttacctc cataaaatca aatacttata cgatagaaga
     6181 ccgggcttta atttgtgcgg aattgaataa gcccgttttt ggagaggtaa attatgggat
     6241 gaatgaattt acgattcaca aaacagactc ttcttccatg attgtaatac ataccttttt
     6301 gaacggagag tttctaaatt ctttttgggc cgatgggtta atcattgcta caccaacggg
     6361 ctctacggct tactcactga gttgtggagg tcctattgtg tttccgaatt ccaataattt
     6421 tgtgattact ccggtggcac cgcataacct caatgcgcgc ccgatgatta taccggataa
     6481 tactgtgttg agttttgaag tagaatgccg aagcaagacg tacttgtgtt ccatagactc
     6541 ccgatctaag agcctggatg atacttttca ggtggcagta cgcaaagccg atttttattt
     6601 caagttgttg cgcatgccgg aaagttcata cctgaaaacc ttgcgcgaaa aactcaactg
     6661 gggcgaggat acgagaaact cttgattatt gtgaagatta cttaataatc tagcccaaat
     6721 atttaccgga aataactacg gcatgcttaa aagcaataaa gtctgtatcc acctcgaaat
     6781 tattttcctg caaaatttga attatatatt gaacgggaac cgtgttctta gcaaatgaat
     6841 ataaatattt atgatggttt tttccggtaa tgagtttatt aataatcggc agaaaatagt
     6901 gcatataatt gagtgccatt ctgcttgaaa agctgcgatt gtctaaacag atttctgcca
     6961 tacagaatct tccctgagca cacaaaaccg ttcttagttg atgaacaaat attggaatct
     7021 catcgtcctg cagctgcttt aaaccaaatg tgcatagaca attattaaat ttcaattcac
     7081 tgatatcatc tgataagaaa tctttcttaa ccaaatggac tgatttggag tatttattgg
     7141 aagctcgtgc aacagacagc atgttttcag aatagtcaag ggcagtaata tggttggtaa
     7201 atctgaagag gtatttaaag ttatcacctg ttccgcacat aatgtccaat acagattcgt
     7261 tttgcttaat ctttaattta tcaatagctt gcttacgaat atatgcagtc agcccgaatg
     7321 aaaatatatt attcagccaa taatcttcag ccaaatcatt aaatatcttc tttattttag
     7381 tttgcctcaa cacgttgttt gattgtctgc aatacatttt tatgcatttg atggataata
     7441 tacctgttcc ataaattcca ataaaaataa ggacgtaaat ggtgggtcaa ttctgtagta
     7501 gcgtataatt cggttttgtt ttcaccaagc tgcttaatca taaattcgcc ataattaatt
     7561 ttaaaatagg catcgtgtga gtgaggagta ttaatgtttt tgtaaacggt taattctttg
     7621 atgggcaata ttgcctgccg tggttcaaac cggagcaaac tgtcttcttt taaggaggtg
     7681 atttttaaag cagcatctcc atttcttaag ttgcacacca gataaagact atcattcgct
     7741 gctgttacct gcatgttttt agggtaattc acaccatact taaagaaaaa attagtgggt
     7801 tctgcatatt gtacaggctg agttaaaaca ttccaaacga tagatgtatc tgcatttatt
     7861 gttagtttgt cttctatatg ttgagtaaat ccatctttca tgcttaaatc aattcctgca
     7921 aaaaacggat ttattaaaat caaagaaaaa aggaaaggtt tctgtgttat attgtaatgc
     7981 actcttatga tatgacctaa tgcataagaa aacaataata agatataaag cggtacggcg
     8041 atcataatta tgcaaatacc accttcaacc ttacatagaa tcaataataa ggtaatcact
     8101 atgaaaatca gcaatacctt attaaatata tgaaaaatac tctttgtcgg gtttttcatt
     8161 ataaatccgg ccgtaaagcc tattgataaa ggaacaccaa taaatagcat aaacccatat
     8221 tcagtatcat ataagatgac taaacacata ctgacaaata cactcagtat tgcaatgaag
     8281 acgatttttc ccattaagct aatactaaca attggattgt tatcatccaa tacagcatca
     8341 tcaaaagttt tcataagacc aattctcttt caaaattaat tgaaacttcc gaaaaaataa
     8401 aaaatatttt taaagtcgct ttatgtcggc tcccagggct tgtaagcggg tatctatgtt
     8461 ttgatagcct ctgtctatt
//
LOCUS       DAOQII010000094         8455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4529_length_8455_cov_4.754167, whole genome shotgun sequence.
ACCESSION   DAOQII010000094 DAOQII010000000
VERSION     DAOQII010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_8455_cov_4.754167"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLF48_05940"
     CDS             complement(<1..905)
                     /locus_tag="PLF48_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48902.1"
                     /translation="MRIYTLLLFCLLYTVGFSKTPLKLNSIRVEDLQVQHAKLAGNSN
                     DKIIFAAKLADKQSAVAIPVTYHIEAYLGDQVFLLSTNYTNINAKQLSTDFVELYQID
                     NSSKIDVNIDRTKKSIPVTILFSSYFLPTDISALANSLGFTIEQTDFIHHHFSAYLSP
                     EQIQKLTEKTDIFYITKYFPQKNILLFDNACIIGQSQINGPIPFGFNLDGSGNNIGIW
                     DEGTVGQNFELPASKNINIDKALNFPPFTTHPTFVGGCIASQGNNFFTVTATSPKSVN
                     FYYDILNDIVNEILSAKTEHQVNIS"
     gene            complement(979..3357)
                     /locus_tag="PLF48_05945"
     CDS             complement(979..3357)
                     /locus_tag="PLF48_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNF48903.1"
                     /translation="MNIYPDNALNKLDFPFIKRELENLCSGSLGKKELHGQAFISNTD
                     ELAHYLQLVNECKNCLQHDAALPQYGYNELNFLNKLNIINYYFQIEECVELYYFLSGF
                     TEWLRFFTPKARQQLYPALYQKINDYSIEIGLIHQITSVIDIEQKQVKENATPELMRI
                     RKSMFEKNHEINAAFRRALNHYRQQQFLIETEETVREGRRVLAVRAEYKRSIKGVILD
                     ESENGNITYIEPNETLFLNNELTELRLEEKREIIKILTALTLKIQPYKEQIDQLQNLM
                     GELDVIKAKAYFALGYDCYLPELSKEKKIYLIDFVHPVLYYHHKKNKKEVVANTFYLD
                     EHKRVLVLSGPNAGGKSIVLKSIGLIQLMLQFGMLIPAKEGSVLSCFEKLFVDIGDEQ
                     SIENDLSTYSSHLSNMNYFIRHANAATLLLVDEMGMGTDPALGGPMAETILDNLYKKK
                     VFAVITTHFHNLKIYAQQTDGIQSGAMSFDKQHLKPSYQLQLGQPGSSFTFEIAKKVG
                     LPDKLIQDATGKTGENKKVLDEVLTDIQTEKQFIKGIRKNVQQKESHLSELTQNYEKM
                     NKELSKDKKRLLKYYEQRLLEKFNEESRNLENEMRKWREEKSDKNKFLEVRKYIDENR
                     EDIEDRLGESEHQTAEQNTAFTIGQQVVIDDGLEVGQILDIKNNQITVGFGSIQTKVK
                     KDRIKPAEPVKTQTVKKQSVNKQMITEKSQFDYHIDLRGLLKEEALPALENFLDRGLM
                     YGFSKMKILHGRGTGALRQAVHHYLKQYKPVKSFYFEEEKFGGDAITIVEMK"
     gene            complement(3413..4201)
                     /locus_tag="PLF48_05950"
     CDS             complement(3413..4201)
                     /locus_tag="PLF48_05950"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNF48904.1"
                     /translation="MNKIKKISFIGSGNVATHLAKAFAKKGIEVVQIYSQTCDNSEVL
                     AWQSNAKYICDLEELDLQVDLLIFAVKDDVIKLLAAQILAKKPDMKVAHTSGSVGLDA
                     FGNAPNSGIFYPLQSFSKTKYIAIAEVPFLITSENELFKNELIELAKIISVNVYEYSD
                     EQRKHLHIAAVFVNNFSNHLFALAQEYCEKHQIDFKLLLPLIHETIYKIDTLPPKMAQ
                     TGPAARKDSEILNKHLAVLEIEDRHLYNIYDMLTQSILNMRDTL"
     gene            complement(4198..5544)
                     /gene="mtaB"
                     /locus_tag="PLF48_05955"
     CDS             complement(4198..5544)
                     /gene="mtaB"
                     /locus_tag="PLF48_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNF48905.1"
                     /translation="MLIFALMLQKKIAFHTLGCKLNFSETSTLARNMQSVGFQVVDES
                     KFSDVYVINTCSVTEQADTKCRNIVRRMMKQNRHAYIVVVGCYAQLKPKEIAEIPGVD
                     LVLGAAEKFKMHEIISDITKNPCGQIQQQEITNANFFVSSYSLGDRTRSFLKIQDGCD
                     YKCSYCTIPLARGKSRSDTMENVLANANTLAANGVKEIVLTGVNTGDFGKHAQERFRF
                     FDLIQELDKIEGIERYRISSIEPNLLTTEIIDFTAKSKKFMPHFHIPLQSGSDKILGL
                     MQRRYKSEVYRKRVEYIKKMMPHAAIGVDVIVGFPGETEQDFLDTYHFLNSLDISYLH
                     VFTYSERPNTKAAEFDSKISSAEKNMRNLQLRNLSEKKKRFFYEQHTGTIRTVLPEEE
                     NKGDVMFGFTDNYIKVAIPYQNDFVNNIVPVNLIDLDDNGNMRASICNAEAKAVAV"
     gene            5611..6633
                     /locus_tag="PLF48_05960"
     CDS             5611..6633
                     /locus_tag="PLF48_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF48906.1"
                     /translation="MIKIGIIGIGRTGSLHLEKLLQSDLYTVVGCFDADKSRLQFIQE
                     EFAIPCFENVEDLISECDALDIASSVDSHLYYCELALRKGKHIFLEKPLSNNLADAKK
                     IMELVLEAGVKFQIGHIDRFNPAFTHTFPEILHPTYIEVNRLKKLDLNKFHVDVVFDL
                     MLNDIDLILKTVKANIKAIKANGSIVLTDTIDIANARIEFDNGCIANLTASRIGNENR
                     SNALFYQKDNLIEIDFLNRTSKITSLSKQEIRLDEKDTFLHPEKKLLQATLDISEETD
                     MLRNAMDAFAMSLYMNKETAVTIHDAYKALEITTEILAQIHKNLSTEERRELDMKPIT
                     NGGCAY"
     gene            complement(6666..>8455)
                     /locus_tag="PLF48_05965"
     CDS             complement(6666..>8455)
                     /locus_tag="PLF48_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48907.1"
                     /translation="IFVLFIHAFAFAQQDSDYDLFLARQFMQNGEFAKAAEYFQDLYS
                     KNQDEYYAEYLQLLLQLKEYEKAEKLIKSQYKYADENPLYLLDLGSLYQMQNVPSAAE
                     KQFNKAIKELKPDRYSIQEIAQRFIKMNQPDLAQKTYLKGKDILRDKSAFNLDLADLF
                     LLKNDFNSMIAAYLDEAPYQANNLSVIETGLQKAMADEKKKDALEKELLKRTQQDKKQ
                     FVYQDLLVWLYMQRNDFDAALLQAKSLDILRNEDGSGVMIIANAALEQKDYDAAIKGY
                     QYVADKGTDMPWYETAGLALINTKREKIVNTADYTYEDLLSLKSSYLNYIQNCKSEKA
                     RLNATLELAQLQALYIHDYDAALALTEPLTDNNSIPKDILAKAKLYTGDYHLLKDEPW
                     ESLLLYTQVEKEQKGNPLGEEAKFRNAKLAYYKGDFEWSQTQLKILKASTSELISNDA
                     IDLSVFILDNLNTDTTDFALMKFSKADLLLYQHKYQDALDTLSLIEKNYFGSSLTDDI
                     LYLKYKIEKQQQHYDKAATYLQKIIDNYAYDLLGDNAIFYLAELNQFYFINEEKSKEL
                     YEKIIIDYKDSTFAIEARKRYRKLRGDVN"
BASE COUNT         2546 a         1568 c         1384 g         2957 t
ORIGIN      
        1 ttactgatat taacctgatg ctcagttttt gctgaaagta tttcatttac aatatcattc
       61 aggatatcat aatagaaatt aacagattta ggtgatgtcg cagtcacagt aaaaaaatta
      121 tttccttgtg aagctataca gcccccaaca aaagtagggt gtgtggtaaa aggtggaaaa
      181 tttaatgctt tatcaatatt tatgtttttt gaagccggta actcaaaatt ctgacccaca
      241 gtaccttcat cccagattcc tatattattg ccactgccat ccagattaaa tccaaatgga
      301 attggtccat ttatctggct ttgtccaata atgcaagcat tatcgaatag cagtatattt
      361 ttttgaggaa aatatttcgt aatataaaat atgtcagttt tttctgtcag tttttggatt
      421 tgttccggag ataaataggc agaaaaatga tggtgtataa aatctgtctg ctcaatagta
      481 aaacctaaac tgtttgctag tgctgaaata tctgttggaa gaaaataaga agaaaataag
      541 attgtaacag gaatggattt ttttgttctg tcaatattta catctatttt actgctgtta
      601 tcaatttgat aaagctcgac aaaatcagtt gataattgct ttgcatttat attggtataa
      661 ttggtagaaa gcagaaagac ttggtcacct aaataagctt caatgtgata tgtcaccggt
      721 attgcaactg ctgattgttt gtcggctaat ttcgctgcaa aaataatttt atcattacta
      781 tttccggcaa gttttgcatg ctgcacctgc aaatcctcaa cacgaatact gtttagtttt
      841 agcggtgttt tagaaaaacc aactgtatac agcagacaaa ataatagcaa agtgtatatt
      901 ctcataccct gaattgttat aacataaata tatggctatt ctttgaaaac cacaagtaag
      961 taaatcgaca atgtataact atttcatttc gacaatagtt atggcatcac cgccaaactt
     1021 ttcttcttca aaatagaatg attttacggg cttgtattgt ttcagatagt ggtgtacggc
     1081 ttgacgcaaa gctccggtac cacgaccgtg taatatcttc attttagaaa atccatacat
     1141 cagtcctctg tccagaaaat tttccaaagc cggcaaggct tcttctttta acaatcctct
     1201 caaatcaata tgataatcaa actggctttt ttctgtaatc atttgtttgt ttacactttg
     1261 tttttttact gtttgagttt taacaggttc tgccggttta attctgtctt tttttacttt
     1321 tgtttgtatg ctaccaaagc ctactgtaat ttgattattt ttaatgtcca atatttgacc
     1381 cacttctaag ccatcatcaa tcactacttg ctgtcctatt gtaaatgctg tattttgctc
     1441 cgcagtttga tgttcagatt cacccaatcg atcttcgata tcttctctgt tttcgtcaat
     1501 atattttcta acttcaagaa acttattttt atcactcttt tcttctcgcc atttgcgcat
     1561 ttcattttcc aggttgcggc tttcctcatt aaatttctct aacaatcttt gttcataata
     1621 tttcagcaat ctttttttgt ctttactgag ttctttattc atcttctcgt aattctgagt
     1681 caattctgag agatgtgatt ctttttgttg cacatttttt ctgatgccct ttataaattg
     1741 tttttctgtt tgaatatctg tcaatacttc atccaatact tttttatttt ctccagtttt
     1801 acccgtcgca tcctgaatta atttatccgg caaacctact tttttagcta tctcaaatgt
     1861 gaaagaactg cccggttgcc ccagttgtaa ttgataagat ggcttcaggt gctgtttgtc
     1921 aaaactcatg gctccgcttt gaattccatc ggtttgttgg gcataaattt tcagattatg
     1981 aaagtgagtt gttattacag caaacacttt tttcttgtac aaattatcca aaatagtttc
     2041 ggccatgggg ccacctaatg cagggtcggt gcccattccc atttcatcaa ccaatagcaa
     2101 tgtagcagca ttggcatgac gaataaaata attcatattg gataaatgcg aactatatgt
     2161 gctcaaatca ttttctatgg attgctcatc accgatatcg acaaacaact tttcaaaaca
     2221 agataaaaca gagccttctt tagccggaat caacatgccg aattgtaaca tcagctgaat
     2281 taatcctatt gattttaaca cgatagactt accgccagca ttgggtccgg ataagactaa
     2341 aacacgttta tgttcatcta aatagaaggt attggcaaca acttcttttt tatttttttt
     2401 atggtgatag tataatacgg gatgtacaaa atctattaaa tagattttct tttctttcga
     2461 caattcgggt aaataacaat catagcccaa ggcaaaataa gcctttgctt ttatcacatc
     2521 caactcaccc atcagatttt gcaattggtc gatttgttct ttgtagggtt ggattttaag
     2581 cgttaatgca gtcagtattt ttattatttc cctcttttct tccagtctta attcagtcag
     2641 ttcattattc aaaaacagtg tttcattagg ctctatatag gtaatatttc cattttcgct
     2701 ttcatccaaa atcacacctt ttatacttcg tttatactct gcacgcactg ccagtactct
     2761 cctgccctca cgaactgttt cttccgtttc tattaaaaat tgttgctgtc tgtaatgatt
     2821 taaggctctt cgaaatgcag catttatctc atgatttttt tcaaacatgg acttacgaat
     2881 acgcatcagc tcgggtgtag cattttcttt tacttgtttt tgttcaatat cgataactga
     2941 ggtaatctga tgtatcaatc ctatttcgat tgaatagtca ttaatttttt gatacaatgc
     3001 aggataaagt tgctgccgtg ctttgggtgt aaaaaagcga agccattctg taaatccact
     3061 taagaagtaa tacaactcta cacattcctc tatttggaaa tagtaattga taatattaag
     3121 cttgttcaga aaatttaatt cattataacc atattgtggc aatgcggcat catgctgtaa
     3181 acaattttta cactcgttta ctaattgcaa atagtgcgca agctcatccg tattggaaat
     3241 aaaggcttgg ccatgcagtt cttttttccc taatgaaccc gaacataaat tttccagctc
     3301 ccttttaata aatgggaaat ctaatttatt cagcgcatta tccggatata tattcatcgt
     3361 gtataaaatc ggactgcaaa aataagaaat aaaacggtgt gaaaaagcga cactacaagg
     3421 tatcacgcat attcaatatg gattgtgtca acatatcata aatattatac aaatgccggt
     3481 cttcaatttc cagcacagct aaatgtttgt tcaaaatttc ggaatctttt cttgcggcag
     3541 gaccggtttg tgccatttta ggcggcagtg tatcaatttt ataaatagtt tcgtgaatta
     3601 aaggcaagag caatttgaaa tcaatctgat gtttttcgca atattcttga gccaatgcaa
     3661 ataagtggtt ggaaaaatta ttgacaaata ctgcggcaat atgcaaatgc ttgcgttgtt
     3721 catcggagta ttcgtatacg ttgacactga taatcttggc taattcaatt aattcatttt
     3781 tgaaaagttc attttcagaa gtaatcaaaa aaggtacttc agcaattgcg atatattttg
     3841 tttttgaaaa agattgcaaa ggataaaaaa tgcctgaatt gggggcattg ccaaatgcat
     3901 caagcccaac agagccggaa gtatgcgcca ctttcatatc cggtttttta gctaagattt
     3961 gagccgccag caattttata acatcatctt taacggcaaa aatgagtaaa tctacttgca
     4021 agtctagctc ttccaaatcg catatatact tagcattgga ttgccatgcc agcacttctg
     4081 aattatcgca tgtttgactg taaatctgaa cgacttctat ccctttttta gcaaatgctt
     4141 ttgccaggtg cgtagccaca tttccagaac cgataaacga tatttttttt atcttattca
     4201 tactgccact gcttttgctt cagcgttaca aatacttgct ctcatgttac cgttatcgtc
     4261 caaatcaatt aagttcacag gtacaatatt attgacaaaa tcattctgat atggaattgc
     4321 aactttgata taattatcag tgaaaccaaa catcacatcg cctttatttt cttcttcagg
     4381 caaaaccgtt cgtatcgtcc cggtgtgttg ttcataaaaa aatcgctttt tcttttcgct
     4441 taaatttctc aattgcaaat tgcgcatatt tttttcagct gatgaaatct tactgtcaaa
     4501 ttcggcggct tttgtattcg gtcgctccga gtaggtaaaa acatgcagat aggatatatc
     4561 aaggctattt aaaaaatgat atgtatctaa aaaatcctgt tctgtttccc ccggaaatcc
     4621 tacaattaca tctactccaa tagcagcatg cggcatcatt ttttttatgt attcaactcg
     4681 cttgcgatag acttcacttt tatatctgcg ctgcattaag cctaaaattt tatcacttcc
     4741 actttgtaga ggaatatgaa aatgcggcat aaatttttta ctcttcgcag taaagtctat
     4801 aatttctgtt gttaataaat tgggctcaat agaggaaatg cgataacgct caataccctc
     4861 aatcttatcc agttcctgaa tcaaatcaaa aaatcgaaat ctttcttgtg catgtttgcc
     4921 aaaatctcca gtatttactc ctgtgagtac aatttctttt actccgtttg cagccaaagt
     4981 gtttgcattt gccagcacat tttccatagt atcactccgg cttttgcctc ttgctaatgg
     5041 aatggtacaa tatgaacatt tataatcgca accatcctga attttcaaaa aagaacgggt
     5101 tcggtcgccc aatgagtaag aagaaacaaa gaaatttgca tttgtaattt cttgttgctg
     5161 aatttgaccg cacggattct ttgttatatc tgaaataatc tcatgcattt taaacttttc
     5221 agcagcaccc aaaaccaaat ctacaccggg aatttctgca atttcttttg gttttaactg
     5281 tgcataacaa cctacaacta ctatatatgc atgtctgttt tgcttcatca tacggcgcac
     5341 aatattgcga catttcgtat ctgcctgttc ggttactgaa caagtattga ttacataaac
     5401 atcggaaaat ttactttcat caactacctg aaatccaaca ctttgcatat tcctagctaa
     5461 ggtagaagtt tctgaaaaat tgagtttgca accgagtgta tgaaatgcaa ttttcttttg
     5521 cagcataagt gcaaaaataa gcattacaca caaattgtgg attaattaat tgagaaacaa
     5581 ttcaataggc ttttatcttt aacttccgta atgataaaaa tcggtataat cggtataggc
     5641 agaacgggaa gtttacattt agagaaacta cttcagtctg atttatatac tgttgtcggt
     5701 tgttttgatg cggataaatc gcgtcttcag tttatacagg aagagtttgc cataccttgt
     5761 tttgaaaatg tggaagattt gatttcggaa tgcgatgcgc ttgacattgc aagttctgta
     5821 gattcacatt tatattactg tgagctggct ttacgtaagg gcaaacacat ttttttggaa
     5881 aagcccttat ctaacaattt agctgatgca aaaaaaataa tggaactggt actggaagcc
     5941 ggtgtaaagt ttcagattgg gcatatcgat cgttttaacc cagcatttac acataccttt
     6001 cctgaaatat tgcaccccac ctatattgaa gtaaataggc ttaaaaaatt agatttaaac
     6061 aagtttcatg ttgatgtcgt atttgatttg atgctgaatg atatcgactt gattttaaaa
     6121 accgtaaagg caaacattaa agcaattaaa gcaaacggtt cgatagttct gacggatacc
     6181 atagatattg cgaatgcgcg aatagaattt gacaacggtt gtatcgccaa tcttacggca
     6241 agcaggattg gcaatgaaaa cagaagcaat gctttgtttt atcaaaaaga caacctaata
     6301 gagattgatt ttttaaaccg aacttcaaaa atcacttctt tatccaagca agaaattcgc
     6361 ttagatgaaa aagacacctt tctgcatcca gaaaaaaaat tattgcaagc cacccttgat
     6421 atctcagaag aaacagatat gctaagaaat gctatggatg cctttgcaat gagtttgtat
     6481 atgaataaag aaacggcagt tacaattcat gatgcctaca aagctttgga aattacgaca
     6541 gagatattag ctcagataca taaaaatttg tcgacagaag agagaagaga actagacatg
     6601 aagccaataa ctaacggagg ttgcgcatat tagtgcctta tcgtactgcg gctttagctg
     6661 catattcaat ttacatcgcc tcgtaatttt ctgtaacgct ttctggcttc aatggcaaat
     6721 gtggaatctt tataatcgat gattattttt tcgtacaatt cttttgactt ttcctcattt
     6781 atgaaataga attgatttaa ttctgccaga taaaatatcg cattatcgcc caataaatca
     6841 tatgcataat tgtcgattat tttttgcaaa taagttgccg ctttatcata atgttgttgt
     6901 tgcttttcta tcttatattt taagtataaa atatcgtcag ttagcgatga accaaaatag
     6961 tttttttcaa taagcgacaa ggtgtccagt gcatcctgat acttatgctg atacagcagt
     7021 aagtctgcct tggaaaattt cattaaagca aaatcagtag tgtcggtatt caaattatcc
     7081 agaataaaaa ccgacaagtc gatggcatca ttcgatatca gttcggaagt agatgctttc
     7141 agaattttca attgagtttg cgaccactcg aaatcgcctt tgtaataggc cagttttgca
     7201 tttctgaact ttgcctcctc tcccagtgga tttccttttt gttctttttc cacttgcgtg
     7261 taaagcaaca aactttccca tggctcatct ttcagcaaat gataatctcc ggtatataat
     7321 ttcgcttttg ccagaatatc tttcggaatg ctgttgttat ctgtcaatgg ctctgtcaac
     7381 gcaagtgctg catcataatc atgtatgtag agggcttgca attgggctaa ttccaatgtg
     7441 gcattaaggc gagctttttc acttttgcag ttttgaatat aattgagata agatgatttt
     7501 aaagaaagca gatcttcata agtatagtct gcggtattca caattttttc tcgtttagta
     7561 ttaatcaatg ccagtcctgc tgtttcgtac caaggcatat cagttccctt atctgccaca
     7621 tattggtaac ctttgatagc ggcatcataa tccttttgct ccagtgcggc attggcaatt
     7681 atcatcacac cgctaccgtc ttcattacga agaatatcca acgattttgc ctgcagcaat
     7741 gccgcatcaa aatcattgcg ttgcatatat aaccaaacca gtaaatcctg atatacaaat
     7801 tgttttttat cttgttgtgt tcgctttaac aattcttttt ctaaggcatc ttttttcttt
     7861 tcgtcagcca ttgctttttg taatcccgtt tcaataacgg ataaattatt tgcctgatag
     7921 ggagcttcgt ccaaataagc cgctatcata ctgttgaaat catttttcag taaaaacaaa
     7981 tcagccaaat ccagattaaa tgcactttta tcacgcaaaa tatcctttcc tttcaagtat
     8041 gttttttgag caagatccgg ttgattcatt ttgataaatc gctgagctat ttcctgaatg
     8101 gaatagcgat ctggttttaa ttcttttata gccttattaa attgtttttc tgctgcacta
     8161 ggtacgtttt gcatctggta taagctgcct aaatctaaca gataaagtgg attttcatct
     8221 gcatatttat actgtgattt gattaatttt tcagcttttt cgtattcttt tagctgtagt
     8281 aagagttgta aatattcggc ataatattca tcctgatttt tgctgtacaa atcttgaaaa
     8341 tattctgcag ctttagcaaa ttcgccattc tgcataaact gacgagccaa aaataaatca
     8401 tagtcgctat cttgctgtgc aaaagcaaat gcatggataa acaatacaaa aatta
//
LOCUS       DAOQII010000095         8454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4530_length_8454_cov_4.344327, whole genome shotgun sequence.
ACCESSION   DAOQII010000095 DAOQII010000000
VERSION     DAOQII010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4530_length_8454_cov_4.344327"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLF48_05970"
     CDS             complement(<1..292)
                     /locus_tag="PLF48_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNF48908.1"
                     /translation="MKIAEIIETIENFAPIPYQEAYDNSGLIIGDRMATCTGVLLCID
                     TIEAVVEEAIAKKCNLIVAHHPIVFSGLKQITGKNYIERVVLKAIKNDIAIYA"
     gene            343..1509
                     /gene="lpxK"
                     /locus_tag="PLF48_05975"
     CDS             343..1509
                     /gene="lpxK"
                     /locus_tag="PLF48_05975"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNF48909.1"
                     /translation="MALITKIVLFPFAVLYGGIIWLRNGLYKSNFIGSTDFEIPVISI
                     GNLSMGGTGKTPFVELLIELLHSEYKMGVLSRGYKRKTSGYIEVHPSHTAKEVGDEPL
                     MLKMKYPDANVAVGEQRVIAIPQLLATHPDVQVIVLDDAFQHQSVRPDINILLTTYQK
                     PFYADAILPLGTLREWESGKVRANIVVVTKCPQAISKTEQEQIRLNLKLNEKQKVFFA
                     TMEYGVPYNILNPSDRFFFDSQKPVLLITGIANAAPLKDFLQTQTKEVIFFKFDDHHF
                     FSEYELENIVKNYAHFSRWITTEKDAVRLALHKDWLIKNNITVYCVPIKTKLIGEGAA
                     EFSKLTKGFLDFFYKTETLTPTDNTKSSENDSATPQSSGDRQKLDNFGAHNPIF"
     gene            1548..2840
                     /locus_tag="PLF48_05980"
     CDS             1548..2840
                     /locus_tag="PLF48_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNF48910.1"
                     /translation="MKTHHKIINGDSRLMAELPDNSVHLAITSPPYWQLKDYGTDNQI
                     GFHDSYENYINNLNLVWKECYRTLNNGCRLCVNIGDQFARAVYYGRYKVIPIREEIIK
                     FCENIGFDYMGAIIWQKVTTSNTTGGGVQMGSYPYPRNGILKLDYEFILVFKKLGDSP
                     KPTREQKELSKMTAEEWNTYFAGHWNFAGARQSGHIAMFPEELPRRLIKMFSFVGETI
                     LDPFAGSGTTALAAKNTDRNSVGFEINPEFIPIIKEKLEVHQKDLNETTYEFIEQKTL
                     KTDFEKEIQKLPYIFKDPHTLDKKIDVKKLQFGSKIDKDSSTKREELFTVKEVISPEI
                     IRLSNDLTVKLIGVKEDPIINGKATSFLIEKTKGKRVFLKYDNVKHDNNNNLLCYLYL
                     ENKTFINAHLIKNGLVQVDSDIDFKYKDKFLNLLRNGI"
     gene            2830..3177
                     /locus_tag="PLF48_05985"
     CDS             2830..3177
                     /locus_tag="PLF48_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48911.1"
                     /translation="MAFNQTDINNLLTVLNNTSRTNPLTAIQIETALQLQFGFPTSGN
                     QQRTRALIKHTIASGHLIKSSTANPAGFWLSNDRQEILTNFESLKRRAFKIMESAIRL
                     KDTWNRNNQNNQI"
     gene            3177..3815
                     /locus_tag="PLF48_05990"
     CDS             3177..3815
                     /locus_tag="PLF48_05990"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HNF48912.1"
                     /translation="MAKEWILNSAMNRFQLNFKRNVGPTSESIRKCSPKNLDEWRAYY
                     FGNVKSKEHIIELGKKLYIKITEVIQAEVADVTEQDCVDYMMQLVIDRTYDGYVTEIQ
                     TIYGQLQGVLKVKIEPAPDEWDRLFNVDFFIKVGEKFIGLQIKPVSFGSGAQIAEIFK
                     EKNIQEETHKQFTEGFGGKVFYIYSIKNGEKKEIYNKEVIDEIAAEIKRLNK"
     gene            complement(3964..5301)
                     /locus_tag="PLF48_05995"
     CDS             complement(3964..5301)
                     /locus_tag="PLF48_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF48913.1"
                     /translation="MPKFSVYFYWLVSCILPIAALAQTTAKESGTAAVKITFSYGNNK
                     DYAGQNTALKADLYKPSLFSSGLPVVILYHGGGYASGDRNVASLKVFSEYFTSQNIST
                     ILPDFRQGWYESSSKPLCESVTAEKFEDAALRAYQDNRALIRYCKANAGALGIDSNKI
                     FLFGISSGGFLVLHHLYISDASISSDRLQRLGSIDGQDNAYTNSTDIAGIISVVGGFY
                     ANNATILKKYPLLLFNNSCDGAVDFFNGWLGNCSNTIRTYGPGIFTKILEQSYTPYSL
                     HVFCGYNHGFNTLAAPQGGDAQAIEYINKKSVDFIQQTIQGTYHYATAVASDSISSVP
                     LGECRNFETFYWCKKDSINTGENSISITPNPISCALQPRLNIRHPNDEVLTIMLVNEM
                     GAILSQQKIEYKTSQSVIYLNVTDFGEGITIVLVKNSMGKTLYKTKVARYCAF"
     gene            complement(5294..5746)
                     /locus_tag="PLF48_06000"
     CDS             complement(5294..5746)
                     /locus_tag="PLF48_06000"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNF48914.1"
                     /translation="MKILMVCLGNICRSPLAEGILKHKCAARNLDWYIDSAGTGKWHL
                     GSAPDKRSIQIAEKHGIHIADQRARSINSEDYQKFDLIYAMDVSNYNDLMRWALDKEE
                     ESKIKLIMQEVYPTENRSVPDPYFDSKGFQKVYTMLDKACDKIIEKYA"
     gene            5833..6663
                     /locus_tag="PLF48_06005"
     CDS             5833..6663
                     /locus_tag="PLF48_06005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNF48915.1"
                     /translation="MQRFIFILFFFLMWVIAPVSARQKWVKRSDGISYKIVTKDTSKA
                     KPVYGDHIWMHLQKIAPSKKEIFNTKIFGMENGVDMEYKVPAKQTDITTFFQKMGKGD
                     SAVVKIPASLMDSAGSARKYYIFKIKLLDFKEKNKHESDKQIRYRQQMIIDSLAIVDY
                     LKNNSIPQHSTDNYGNIFWRTQYGSEREVIAGDTVHLQYVGKLLNQKEFDNSYKRNQP
                     LNFIVGKKQVIDGLDLGICNFHHGDKGILIIPSRLAYGEREAGAIPENSVLLFELDIQ
                     "
     gene            6822..8276
                     /locus_tag="PLF48_06010"
     CDS             6822..8276
                     /locus_tag="PLF48_06010"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF48916.1"
                     /translation="MAKNTYDVIIMGAGLSGIGTAYHLQQECKGKTYVILENRESIGG
                     TWDIFRYPGIRSDSDMFTLGYSFKPWKYNEAIADGGLILNYIKETAHENGIDKNIHFN
                     TKINRADWDSNTNTWTVTCYNSKTKKTVEYTAKYVISCLGYYNYEKAHRPEFKGEKSF
                     KGDILHPQFWPKNYDYSGKEVVVIGSGATAVTLVPSMTDKAKHVTMLQRSPTYIASIP
                     NRSLLPQNLLKNLPDTVQYNLNRAAQIGLQVVSYNLCQNQPELMKRYFRAHVKRQLPK
                     GYDIDTHFTPKYNPWDERLCAVPNGDLFKSIRSGKASVITDHIDKFVEDGILLKSGKK
                     IKADIIITATGLELQMLGGIKTYMDGKELKPADGIVYKGLMIKDVPNFAFILGYTNSS
                     WTLKADMVGEYFCKLINTAEKKKNKVFVVKSDKPIEKEPALNFNAGYVQRAIQAGVLP
                     SQGAAYPWKLKQNYLVDAFNFKFRDIKDEFLQFA"
BASE COUNT         2854 a         1504 c         1653 g         2443 t
ORIGIN      
        1 aggcgtaaat agcaatgtca tttttaatgg ctttcagcac cactcgctcg atgtaatttt
       61 ttccggtaat ttgttttaag ccggaaaaaa caataggatg atgtgcaaca atcagattgc
      121 attttttggc aatagcctct tccactactg cctctatggt gtcgatgcag agcagcacgc
      181 cggtgcaagt tgccattctg tcacctataa tcaatccgga gttatcatac gcttcttggt
      241 atggaattgg tgcaaaattt tctattgttt caattatttc ggctattttc atacagttat
      301 ttataaaacg aatttagcga tttttgaatt agatgagtaa agatggcttt aattactaaa
      361 atagtactct tcccgtttgc cgttttgtat ggtggtatta tttggctgcg caatggcttg
      421 tacaaatcta atttcattgg ctctaccgat tttgagatac ccgttattag cataggaaat
      481 ttgagcatgg gcggcaccgg aaaaacgcca ttcgtagaat tattgattga attgctacat
      541 agtgaatata aaatgggtgt actgagccga gggtataaac gaaaaactag tggatacata
      601 gaagtacatc cttcccatac cgctaaagag gtaggagatg agccgctgat gctaaaaatg
      661 aaatatcccg atgcgaacgt tgcagtaggt gaacaacggg ttattgctat tcctcaatta
      721 cttgctacac atccagatgt acaagtgatt gtattagatg atgcttttca acatcagagc
      781 gtgcgccccg atataaatat tttgctgacg acttatcaaa agccttttta tgctgatgca
      841 attttacctt taggcacctt gcgggaatgg gaatccggaa aggtgcgtgc aaatatagtg
      901 gtggttacta aatgcccgca agcgataagc aaaaccgagc aagagcagat tagattaaat
      961 ttgaaattaa atgaaaaaca aaaggtgttt tttgccacta tggaatatgg cgtgccatat
     1021 aatattttaa acccatcgga tagatttttc tttgattcgc aaaagccggt tttgttaatc
     1081 accggaattg caaatgctgc tcccttaaaa gattttttgc aaacccaaac gaaagaagtc
     1141 atctttttta agttcgacga ccatcatttt ttttccgaat atgaactgga aaatatagtg
     1201 aaaaattacg ctcatttttc tcgttggata acaacagaaa aagatgcggt gcggttagca
     1261 ttacacaaag attggttgat caaaaacaat atcaccgttt attgtgtacc tataaaaact
     1321 aaattaatcg gagagggtgc tgccgaattt tctaaattga caaaaggttt tttagatttt
     1381 ttctataaaa cagaaacact aaccccaacc gacaatacca agagtagtga aaatgacagt
     1441 gcgacaccgc aatcatctgg cgataggcaa aaacttgata attttggtgc gcataaccca
     1501 atattttagg taaatttggt cgaaataaaa atttaatata agaagacttg aaaacacatc
     1561 ataaaataat aaacggagac agcagactta tggcagaatt gcccgataat tcggtgcatt
     1621 tggctataac ttctccacct tactggcagt taaaagacta tggtacagac aaccaaattg
     1681 ggttccacga tagctacgaa aattatatca ataatttgaa tttggtttgg aaagagtgct
     1741 accgtacgct gaacaatggt tgcaggttgt gtgtgaacat tggcgaccaa tttgctcgag
     1801 cagtttacta cgggcgttac aaagttattc ctattcgtga agaaatcatc aagttctgcg
     1861 agaacatcgg ctttgactat atgggggcta ttatttggca aaaggtgacg acttcaaaca
     1921 ccacaggcgg tggcgttcaa atgggttcgt atccatatcc aagaaacgga attttgaagt
     1981 tagattacga atttattttg gtgtttaaaa aattaggcga cagtccgaaa ccaaccagag
     2041 aacaaaaaga actttccaaa atgactgccg aagaatggaa cacctatttt gcagggcatt
     2101 ggaattttgc aggagcaaga caaagcgggc atattgcaat gtttcccgaa gaattgccaa
     2161 gacgactaat caaaatgttt tcgtttgttg gcgaaactat tcttgaccca ttcgcaggaa
     2221 gcggaacaac tgcattggca gcaaaaaaca cagatagaaa ttcggttggt tttgaaatca
     2281 atcccgaatt tatcccaatc atcaaagaaa aattggaagt tcatcagaaa gatttgaacg
     2341 aaacgaccta cgagtttata gaacaaaaaa cattgaaaac cgactttgaa aaggaaattc
     2401 aaaaactacc ttacattttc aaagacccgc atacactaga caagaaaatt gatgtaaaaa
     2461 aacttcaatt cggctctaag attgacaagg atagttcaac aaaaagagaa gaacttttta
     2521 ctgttaaaga agttatcagc cctgaaataa ttcgtttaag caacgacttg acggtaaagc
     2581 ttattggagt aaaagaagat ccgataatta acggcaaagc aacatcattc ctgattgaaa
     2641 aaacaaaagg gaagcgagta tttctaaaat atgataatgt aaagcacgac aacaacaata
     2701 acttgctttg ctatctctat cttgaaaaca aaacattcat aaatgcacac ttaatcaaaa
     2761 atggtttggt gcaagtggac agcgatattg atttcaaata caaagacaaa tttttaaatc
     2821 ttctgcgaaa tggcatttaa tcaaacagac attaataatc tgctaactgt gcttaataat
     2881 acaagccgaa ctaacccatt aacagctatt cagatagaaa ccgcacttca acttcagttt
     2941 ggatttccca caagtggtaa tcaacaacga acaagagcat taataaaaca tactattgca
     3001 agtgggcatc ttataaaaag ttctactgca aatccggcag gattttggct ttcaaatgat
     3061 agacaagaaa ttttaacaaa ttttgaatct ttgaaaagaa gagcttttaa aattatggaa
     3121 agtgcgatta ggctaaaaga tacttggaat agaaataatc aaaataatca aatataatgg
     3181 ctaaagaatg gattttaaat tcagcaatga atcgtttcca actaaatttt aaaagaaatg
     3241 ttgggccaac ttccgaatca atacgaaaat gttccccgaa aaaccttgat gaatggcgtg
     3301 cgtattattt tggtaatgta aaatcaaaag aacatataat tgaactcggc aaaaaactct
     3361 acattaaaat aactgaagta attcaggcag aggttgctga tgtaaccgag caagattgtg
     3421 ttgactatat gatgcaactc gttattgaca gaacttatga tggttatgtt actgaaattc
     3481 aaacaatcta tggacagcta caaggcgttt tgaaagtaaa aattgaacca gctccagatg
     3541 aatgggatag attgttcaat gttgattttt ttattaaagt gggtgaaaaa tttataggac
     3601 ttcaaatcaa acctgtttca tttggaagtg gtgctcagat agctgaaatt ttcaaagaaa
     3661 aaaatataca ggaagaaacg cataaacaat ttaccgaagg atttggtggc aaggtttttt
     3721 atatctattc aataaagaac ggagaaaaaa aggaaattta taataaagaa gttattgacg
     3781 agattgcagc agaaatcaaa cgactgaaca agtgaaaacc tccttacctt aaacctatcg
     3841 ctttgaatat taaccttatt gtttaaaagt tggtatccac tactattttc ttacttttag
     3901 atgaacagat gtaactttcc actgttttct tagataatta ccaattattt ctcagcagat
     3961 tagttaaaac gcacaatatc gggcgacttt cgttttatat aaagttttgc ccatactgtt
     4021 ttttactaaa actatcgtga tgccttcgcc aaaatctgtt acatttagat agataacact
     4081 ttgcgatgtt ttgtattcta ttttttgttg agatagaatt gcacccattt cgtttaccag
     4141 cattatcgtc agcacttcat cgttgggatg gcgaatattt agccgcggtt gcaaagcaca
     4201 agaaattgga tttggcgtaa tactaatact attttcgccg gtatttatac tgtctttttt
     4261 gcaccaataa aaagtttcaa aattcctaca ttcgcctaat ggtacggatg agatagaatc
     4321 actggcaacg gcagtggcat agtggtaagt accttgaatt gtttgttgta taaagtcaac
     4381 ggatttttta ttgatatact ctattgcttg tgcatcgccg ccctgcggcg ccgcaagggt
     4441 attgaaccca tgattgtatc cgcaaaatac atgcaggctg taaggggtat aagattgctc
     4501 taaaattttg gtgaatatac ccggcccgta agtgcgaatg gtattactgc aatttcccag
     4561 ccaaccgtta aaaaaatcga cagccccgtc gcaggagtta ttgaaaagca atagcggata
     4621 tttttttagg atagtggcgt tatttgcata aaaacctcct acaacactta taattcctgc
     4681 aatatctgtc gagttggtat aggcattatc ttgcccgtca atacttccta atcgctgcaa
     4741 cctgtcgctg ctaatggagg catcggaaat atagagatga tgcagcacca aaaagccgcc
     4801 ggagctgatg ccgaataaaa atattttatt ggagtcgatg ccgagcgcgc cggcatttgc
     4861 tttgcaatac cgaattagcg cacgattgtc ttgataggca cgcagtgcgg catcttcaaa
     4921 tttttccgcc gttacgctct cacacaaggg ctttgaagaa gactcgtacc agccttgcct
     4981 aaaatcgggg agtatggtac tgatgttttg cgaggtaaaa tattcggaaa aaactttcaa
     5041 acttgcaaca ttgcgatcgc cggaagcata tccgccgcca tggtacaaaa ttaccaccgg
     5101 caaccccgaa gaaaataaag aaggtttata gaggtcggct ttcaatgctg tattttgccc
     5161 cgcataatct ttgttgttcc cgtacgaaaa tgtaatcttc accgccgccg ttccactctc
     5221 tttggcggta gtctgtgcaa gcgcagcaat tggcaaaatg caagatacaa gccaataaaa
     5281 gtacactgaa aatttaggca tatttctcta tgattttatc gcaagcttta tctaacatcg
     5341 tatatacttt ttgaaaccct ttactgtcaa aataggggtc gggaacgctc cggttttcag
     5401 taggatatac ctcctgcata attaatttta ttttgctttc ttcttcttta tctaaagccc
     5461 aacgcattaa atcgttgtag ttagaaacgt ccattgcata tatgagatcg aacttttgat
     5521 aatcttcact gttaatgctg cgggcacgtt ggtcggcgat atggattccg tgtttttctg
     5581 caatttgtat agagcgtttg tcgggtgcac tgcccaagtg ccatttgccg gttccggcac
     5641 tgtcaatgta ccaatctaaa tttcgcgctg cgcatttgtg ttttagtatg ccctctgcaa
     5701 gtggcgagcg gcagatattt cccaaacaga ccattaaaat tttcatggta tttattttat
     5761 aaatattttt aacagataag gttattgatt ttatttcgta acaaaaatga ttgttttatc
     5821 ttacaacaca aaatgcaacg gtttattttt atactattct tttttttgat gtgggtaatt
     5881 gctcccgtat ctgcccgaca aaaatgggtg aaacgctcgg atggtataag ctataaaata
     5941 gttaccaagg atacttccaa agcaaagcct gtttatggcg accatatttg gatgcatttg
     6001 caaaaaatag ccccaagtaa aaaagaaatt ttcaatacca aaatttttgg aatggaaaat
     6061 ggcgtagata tggagtataa agtgccggca aaacaaacgg atattactac tttttttcaa
     6121 aaaatgggaa agggagattc tgctgtagtg aaaattccgg caagcctgat ggacagtgcc
     6181 ggcagtgcta ggaaatatta tattttcaaa ataaaattat tagactttaa agaaaaaaat
     6241 aagcacgaat ccgataagca aatccgatat cgacagcaga tgattataga ttctttagcc
     6301 atagtagatt atttgaaaaa caactccatt ccgcagcatt ctacggataa ttacggcaat
     6361 atattttggc gtacacaata tggcagtgag cgcgaagtga tagccggcga caccgtacac
     6421 cttcagtatg tcggaaaatt attaaaccaa aaagaattcg ataattctta caaaagaaac
     6481 cagccgctaa attttattgt aggaaaaaaa caagtgattg acggattgga tttggggatt
     6541 tgcaactttc accacggaga taaaggtata ttgataattc cgagccgctt agcctacggc
     6601 gaaagagaag caggcgccat tcctgaaaat tcagtgctgc tttttgaact ggatatacaa
     6661 taatttaggg aggtaaaact cgcgtagata tacctgttag caggagaaac ggcaatacat
     6721 tttgctcgaa ttttcttaag ttaagtaatt tatttaatta tccatagtaa attatactta
     6781 gcctgacgaa tgcttatttt tgagtattaa aatatctcgt tatggcaaaa aatacttatg
     6841 atgtaataat aatgggcgcc ggtttatcgg gcattggcac ggcttaccac ttgcagcaag
     6901 aatgcaaagg gaaaacctat gttattttgg aaaacagaga aagcattggc ggaacttggg
     6961 atatatttag ataccccggt attcgttccg attcagatat gtttacctta gggtatagct
     7021 tcaagccatg gaaatataat gaagccatcg ccgatggcgg tttgatatta aactatataa
     7081 aagaaactgc tcacgaaaat ggaattgata aaaacattca tttcaatacc aaaataaaca
     7141 gagcagattg ggacagcaat accaatacgt ggacggttac ttgctataac agcaaaacga
     7201 aaaagaccgt tgaatatact gcgaaatatg tcatctcttg cttggggtat tataattacg
     7261 aaaaagcaca tcgccctgag tttaaaggag aaaaatcatt caaaggcgat atactacatc
     7321 cacaattttg gcctaaaaat tacgattatt ccggcaaaga agtagtcgta atcggtagtg
     7381 gagctacggc ggtaacctta gttccttcca tgaccgataa agctaagcat gtaacgatgt
     7441 tgcagcgctc tccaacgtat attgccagca ttcccaatag aagtttgctg ccccaaaatt
     7501 tactgaaaaa cctgccggat accgttcagt ataatttaaa ccgtgcggca caaatcggat
     7561 tgcaggtggt gagctataat ttatgccaaa accaaccgga gctaatgaaa cgctatttca
     7621 gagcgcatgt aaaaagacaa cttcctaaag gctacgacat cgatacacac tttaccccaa
     7681 aatacaaccc gtgggatgaa aggctgtgtg ccgtgccgaa tggcgatttg ttcaaatcga
     7741 ttagaagcgg caaagcaagc gttatcaccg atcatattga taaatttgta gaagacggca
     7801 tactgttaaa atcagggaaa aaaataaagg ccgacatcat cattacggcg acaggattag
     7861 aattgcaaat gctgggcgga ataaaaacgt atatggacgg taaagaactt aaacctgccg
     7921 atggaattgt ttataaagga ttaatgataa aagacgtgcc gaatttcgca tttattttag
     7981 gctacaccaa ttcctcttgg acgttaaaag cagatatggt aggcgaatat ttctgtaaat
     8041 taataaacac ggcagagaag aaaaagaata aagtgtttgt agtgaaatca gataaaccta
     8101 ttgaaaaaga accggcgctt aattttaatg ccggctatgt acaacgcgct attcaagcag
     8161 gggtgttgcc gagccaagga gctgcatacc cttggaaatt aaaacaaaac tatttggtag
     8221 atgcctttaa ttttaaattc agagatataa aagatgagtt tttgcaattt gcatagctca
     8281 tgttatcggt agttatattc gtcaggtggc actataaagt cgcctgacga ataattacgt
     8341 tcctttccaa ttatcccgaa tgcttatcgt ttgaaccctg aaaaactaag agttaaaaac
     8401 taaaaattaa aaattaaaaa ttaaaaatta aaaattacga attacgaatt aaaa
//
LOCUS       DAOQII010000096         8302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4653_length_8302_cov_4.198133, whole genome shotgun sequence.
ACCESSION   DAOQII010000096 DAOQII010000000
VERSION     DAOQII010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4653_length_8302_cov_4.198133"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLF48_06015"
     CDS             <1..504
                     /locus_tag="PLF48_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48917.1"
                     /translation="RDIQTRLFTVYRKFKKFPNKPHNPVSLEQAKKIGILFNAEDVKD
                     NDSILAFSEKLKSSNKDVQLLGFIDHREFGNPYPFPFFTAKDLSWFGKPAGGTCGYFM
                     HFPFDLLITFTKSENQPLDYISASSSAKLRVGFFSADNTSDYDIVFLPSQNKITHCIN
                     SIETYLK"
     gene            586..1473
                     /gene="dapA"
                     /locus_tag="PLF48_06020"
     CDS             586..1473
                     /gene="dapA"
                     /locus_tag="PLF48_06020"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNF48918.1"
                     /translation="MINLKGVGVALVTPFTEQKTVNFSEFEKVVNYVIENGVDYVVVL
                     GTTGESVTLNKQEKQDLIKACVAVTNKRVPVIAGFGGNDTQSTITDIQSYQLDGVDGI
                     LSVSPYYNKPTQEGIYEHYKAIAINTDLPIILYNVPGRTASNIRASTTLQLAHDFKNI
                     VGIKEASNDWMQLFWLAREKPEDFYLISGNDDLIVPQISIGYDGVISVIANATPKLFS
                     DMVHQALNNEYAAARKIVYQIDELITYLFEQGNPAGVKCALQHLGVCADHVRLPLVPV
                     NQDLRDRIGKKMELLGVEA"
     gene            1592..1981
                     /locus_tag="PLF48_06025"
     CDS             1592..1981
                     /locus_tag="PLF48_06025"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNF48919.1"
                     /translation="MDSSNSFPVFPLIFGLGIMVFFIYCMWKIFEKAGHEGWKCLIPI
                     YNAYIQLKIAQMPGWYLLLLLIPFVNIVIAIMMSIKIAENFGKSSGFGLGLAFLGFIF
                     YPILALGDAQYLPLLTNNDPSEHLIEK"
     gene            complement(1982..3160)
                     /locus_tag="PLF48_06030"
     CDS             complement(1982..3160)
                     /locus_tag="PLF48_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNF48920.1"
                     /translation="MLNLNDTQIAFAYKSNHELVRDYQLFRLINQNWLVRWGTQTASK
                     LIDMGLKTPVAWGMRPTIYATFCGGENLEDAQKKINRLFEYKVHSILDYGVEGKETEA
                     DFERTFNAILKSIHYASKNEAVKIVCAKFTGLVPFSILEKLDAEQALSADEEGVFAKF
                     KSRVHQLAQAAYEKNISLFVDAEETWIQQPLDDITFELMLEFNQKRPVIYNTTQHYRK
                     DRMVFLQKEIEKAKEHNIIYAVKLVRGAYMEKENLRAAKLNYACPIQDSKAATDHDYD
                     EAVKLCLQNIKHCAVCIATHNENSTLLATRLMEERQLEAQHPHIQFSQLYGMSDNITF
                     NMANAGYNAHKYLPYGPVKDVIPYLIRRAQENTSVSGQMGRELKLLREEVKRRKLITF
                     "
     gene            3281..5230
                     /gene="acs"
                     /locus_tag="PLF48_06035"
     CDS             3281..5230
                     /gene="acs"
                     /locus_tag="PLF48_06035"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNF48921.1"
                     /translation="MYSYQIKTYEEYKEAYRQSVEDPQKFWWNIAKEFRWMTPPEADC
                     LKWDFKAPQVSWFKGAKLNITENCLDRHLETDGNSPAIIWEPNDPDDHHHYRVLTYKD
                     LHFKVCQFAHVLKNNGIQKGDRVCIYMGMVPELAIAVLACARIGAIHSVIFGGFSAQS
                     IADRLIDAQAAFIITQDGAYRGNKEIPLKSVIDDAIVQCPFVKKIIVLTHSRLPISMI
                     KGRDVWWENEIKKVETQGNPDFPAVEMDAEDPLFILYTSGSTGKPKGVVHTCGGYMVY
                     TNYTFVNVFQYQKGDIHFCTADIGWITGHSYIVYGPLSAGATSLMFEGVPTYPDAGRF
                     WEIVDLYKVNILYTAPTAIRSLMAYGDAPLQGRDLSSLKVLGTVGEPINVEAWNWYNE
                     KIGKQRCPIVDTWWQTETGGIMISNIAGITPQKPTFATLPMPGVQVVLMDENGDELPQ
                     SHSAKADLPESIIKGNLCVSFPWPGILRTTYGDHERCRTNYFATYADKYFTGDGCFRD
                     EHGFYRITGRVDDVLNVSGHRIGTAEVENAINRYIDVVESAVVGYPHDIKGQGIYAFV
                     ILQNELHDAELTKQDIIQTVNRIIGPIAKPDKIQFVIGLPKTRSGKIMRRILRKIAEG
                     ETSNLGDTSTLLDPSVVDEIKNGAI"
     gene            5317..5490
                     /locus_tag="PLF48_06040"
     CDS             5317..5490
                     /locus_tag="PLF48_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48922.1"
                     /translation="MKIEIFEFPPESEALKNKKTHSKNDLTAQQTDSNKSNHKKRNTE
                     KIKSEKNSKQSSG"
     gene            complement(5646..6245)
                     /locus_tag="PLF48_06045"
     CDS             complement(5646..6245)
                     /locus_tag="PLF48_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF48923.1"
                     /translation="MTITLQNISKRFGFEWIFKQIQFSFEEGGKYAVVGPNGSGKSTL
                     LKIISGGLTPTSGKVDFGELEEIAVQKIVYAAPYISLIEDFTLEEMYKFHAAFKTFYT
                     SEEAEFYAISELEKHKTKYIKNFSSGMRQRLKLTFAFLTQSELILLDEPTSNFDTKAV
                     DWYLSMKEKYVGKRTLIIGSNQAYEYEDCSLQLDIMQYK"
     gene            complement(6393..7166)
                     /gene="lpxA"
                     /locus_tag="PLF48_06050"
     CDS             complement(6393..7166)
                     /gene="lpxA"
                     /locus_tag="PLF48_06050"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNF48924.1"
                     /translation="MATNFIHKNAHLGTNVQVEHFSTIYDDVSIGDNTWIGPNVTIFP
                     GTRIGKNCRIFPGAVLGAVPQDLKFAGEYTTVEIGDCTTIRECVTINRGTSALGKTSI
                     GSNSLLMAYVHIAHDCTIGNHCILANVTNIAGHVTIDDYAYFGGLSGAHQFTRIGKYS
                     FISGGSMIGKDVPPYCLVMRNPAQYAGINSVGLRRAEFSRDDIHQIQDIYRIIFSNNG
                     LNTTQALQKIEEEIQPSDIRNYIIDFVKTSERGIIKGMD"
     gene            complement(7211..8134)
                     /gene="lpxC"
                     /locus_tag="PLF48_06055"
     CDS             complement(7211..8134)
                     /gene="lpxC"
                     /locus_tag="PLF48_06055"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNF48925.1"
                     /translation="MILQKTIVKSFSFSGNGLHTGVFSTVTVHPAPENHGIVFCRTDL
                     DRAMIPAQSKYVTNTNRSTTLTNELVDVITVEHLLSALYAKGIDNALIEIDNLELPIL
                     DGSCSCFIKNLNDDNIIELNEPKRFQKIPDVVEWTDEDTGAEYILLPHEDFSVTCLID
                     FPSKLIKHQYAVMNHWSEYTSEIANAKTFCFLHEIEFLYNKGLIKGGNLTNALVFSES
                     TISNEKANWLAKTFHQQANSIPEMGVLNPLFQSYDNEAARHKILDVIGDLALTQTYFE
                     GKLICYKPGHTSNVKFAQFLVDKYIHTSVTV"
BASE COUNT         2583 a         1458 c         1593 g         2668 t
ORIGIN      
        1 agagatattc aaacccgttt gtttactgtt tatcgaaaat ttaaaaagtt tcccaataag
       61 ccgcataatc ctgtttcatt ggagcaggct aaaaaaatcg gcattttatt taatgcagaa
      121 gatgtcaaag acaatgattc tatcctagcc ttttcagaaa aattaaaatc aagcaacaaa
      181 gatgttcagt tgctgggctt tattgaccac cgtgaatttg gcaatcctta tccatttcct
      241 ttttttaccg ccaaagactt gagttggttt ggtaagccgg ccggaggcac atgcggctat
      301 tttatgcatt tccctttcga tttattaatt acttttacta aaagcgaaaa tcagcctttg
      361 gactatatct ccgcttcctc ctccgctaaa ctcagagttg gtttttttag tgcagataac
      421 acctcagatt atgatatcgt ttttcttcca tcgcaaaata aaatcacaca ctgtataaat
      481 agtattgaaa catatctgaa ataatttact ttatagtaac atttttaatt ataatttaaa
      541 ctgtcatttt gtaacttgta aacgaatctc aaaaaatagc tgcaaatgat aaatctcaaa
      601 ggtgtaggtg tggcattggt aacaccattt accgagcaaa aaacggtcaa cttttccgag
      661 tttgaaaagg tagtaaatta cgttattgaa aatggcgtag attatgtggt tgtattaggc
      721 acgaccggcg aatctgtaac actgaacaag caggaaaagc aagatttaat aaaggcttgt
      781 gtggcagtta ccaataaacg tgttcctgtt attgcaggct ttggtggcaa tgatacacaa
      841 agtacaatta ctgatataca gtcctatcag ctggacggtg tagacggcat cttatcggta
      901 agtccctatt ataataaacc tacacaagag ggtatttacg aacattataa agcaatagca
      961 atcaatacgg atttgcctat tattttatat aatgtaccgg gaagaacagc ttctaatatt
     1021 cgtgcttcta cgaccttgca attagcccat gattttaaaa atattgtggg aataaaagaa
     1081 gcgagcaacg actggatgca gctattttgg ctggcacgcg aaaagccgga agatttttat
     1141 ctcattagcg gaaatgatga tttgattgtg ccgcaaattt ctatcggata tgacggtgtt
     1201 atttctgtaa ttgcaaatgc tacccctaaa ttgttttcgg atatggtgca tcaggcatta
     1261 aataatgaat atgctgctgc cagaaaaatt gtttatcaga ttgatgaact gattacttac
     1321 ttatttgaac aaggtaatcc ggcaggcgta aaatgtgcat tgcagcatct tggtgtttgt
     1381 gccgatcatg tgcgacttcc tctggtgccc gtcaatcagg atttgagaga caggataggc
     1441 aaaaagatgg aattgcttgg cgttgaggct taggtatatt taatatctta agtatttttc
     1501 tattcccaaa ttttatcatg ttgataaagt ttttttacta tatacatagg taagtcaatt
     1561 attgtttata tctttaaaaa aaaataaaat tatggattca tcaaacagct ttcccgtgtt
     1621 tcccttaatc tttgggctgg gaattatggt ctttttcatt tattgcatgt ggaaaatttt
     1681 tgaaaaagca ggtcatgaag gttggaaatg cctaattcca atttacaatg cttatattca
     1741 attaaaaata gctcaaatgc caggttggta tttgttgctc ttacttattc catttgtaaa
     1801 tatcgttatt gccattatga tgagtattaa gattgctgaa aattttggca agtcaagtgg
     1861 cttcggatta ggattggcat ttttagggtt tatattctat ccaatattag cattaggtga
     1921 tgctcaatat ttgccgcttt taactaataa tgatccgagt gagcatctga tagaaaaata
     1981 atcaaaacgt aatcaatttt ctgcgtttca cttcttcacg taggagcttt aattccctgc
     2041 ccatttgtcc ggaaacactg gtgttctcct gagctctgcg aatcagatac gggataacat
     2101 ctttcacggg gccataaggc aaatatttat gagcattgta acccgcattt gccatattga
     2161 aggtgatatt atcgctcatg ccatataatt gcgaaaactg aatatgtgga tgttgagctt
     2221 caagttgcct ttcttccatc aaacgggtag ctaatagcgt gctgttttca ttatgagtgg
     2281 ctatacatac agcacaatgc tttatgtttt gcaggcacaa ttttacagct tcatcatagt
     2341 catggtcggt agcagctttg ctgtcttgta ttgggcaggc atagttaagc tttgcagcac
     2401 gcaggttttc tttttccata taagctccgc gtactaattt tacggcataa ataatattat
     2461 gctctttagc tttttcaatt tctttttgta aaaataccat tctgtcttta cggtaatgct
     2521 gagtagtatt gtaaataacc ggacgctttt ggttgaattc cagcatcaat tcaaacgtaa
     2581 tatcatctaa tggttgctgt atccatgttt cttctgcatc cacaaacaaa ctgatgtttt
     2641 tttcgtacgc tgcttgcgca agctgatgca cacgagattt aaattttgca aaaacacctt
     2701 cttcgtcagc agataaagct tgttcggcat ccagcttctc taagatgctg aatggcacta
     2761 atccggtgaa ttttgcacaa actattttta cagcttcgtt cttggatgca taatgaatag
     2821 attttagaat ggcattaaat gtgcgttcaa aatcagcttc ggtttctttg ccttctacac
     2881 cgtaatccag tatagaatgc actttgtatt cgaataaacg attgattttt ttttgtgcat
     2941 cttccaaatt ctcgcccccg caaaaagtgg cgtaaattgt aggccgcata ccccaggcta
     3001 cgggtgtctt caagcccata tcaatcaatt tacttgcagt ttgtgtgccc caccttacca
     3061 gccagttttg attaatcaat cgaaataatt gataatctcg taccagttcg tgattggatt
     3121 tataagcaaa agcaatttgg gtatcattaa ggttgagcat atgggtcata tttcaagtta
     3181 agtactttac aaaaatacga aaagagtagc atgcattttt atcctgtttg gaattacggc
     3241 aatactattt tttttatttt tacttaccta aaaaaaatac atgtactctt atcaaatcaa
     3301 aacttacgag gaatataaag aagcgtatcg gcagagtgtg gaagatccgc aaaaattttg
     3361 gtggaatatc gctaaagaat tccgatggat gacacctccg gaggctgact gtttgaaatg
     3421 ggattttaaa gcacctcagg taagttggtt taaaggggcg aaactgaata tcaccgaaaa
     3481 ttgtttagac cgacatcttg aaacagatgg gaattcgcct gccataatct gggaacccaa
     3541 cgatccggat gaccatcatc actatcgagt tttgacatat aaagatttgc attttaaagt
     3601 ttgtcagttt gcacatgttt taaaaaataa cggcatacaa aaaggcgaca gagtatgtat
     3661 ctatatgggt atggtacccg aactggctat agcggtgttg gcatgtgccc gcattggtgc
     3721 tattcacagt gtcatttttg gtggctttag cgcacagagt attgcagatc gtctgattga
     3781 tgcacaggca gcgtttatta ttacgcaaga tggtgcatat cgtgggaata aagaaattcc
     3841 tctaaaatcg gtgattgatg atgccattgt acaatgtcct tttgtgaaaa aaattattgt
     3901 actcactcat tcacgcttgc ccatcagtat gataaaaggg agagatgtat ggtgggaaaa
     3961 tgaaattaaa aaagtagaaa ctcaaggtaa ccctgatttt ccggctgtag aaatggatgc
     4021 tgaagatccg ttgtttatat tatatacgag cggaagcaca ggcaaaccca aaggagtagt
     4081 gcatacctgc ggaggctata tggtgtatac caattatact tttgtaaatg tttttcaata
     4141 tcaaaaagga gatattcatt tttgtacggc tgatatcggc tggattacag gacacagcta
     4201 tattgtgtac ggtcctttaa gcgccggagc tacatcactc atgtttgaag gtgtacccac
     4261 ttatcccgat gccggaagat tttgggaaat tgttgacctg tataaagtta atatactcta
     4321 tactgcccct acggcaatac gcagtttgat ggcctacgga gatgcccctt tgcagggtag
     4381 agatttgagc tctttgaaag tattaggcac cgtaggtgag ccgataaatg tagaagcatg
     4441 gaactggtat aatgaaaaaa taggcaagca aagatgcccg atagtggata catggtggca
     4501 aaccgaaacc ggcggaatca tgatttcgaa cattgccgga attactcctc aaaaacctac
     4561 ttttgctacc ttgcccatgc ccggtgtaca agtagtttta atggacgaaa atggtgatga
     4621 attaccacaa agccattctg ctaaagctga tttgccggaa tcgatcatca aaggaaattt
     4681 atgtgtgagt tttccttggc ccggtatttt gcgaactact tatggcgatc atgaacgatg
     4741 ccgtacaaat tatttcgcca cgtatgccga taaatatttt acaggcgacg gttgttttcg
     4801 ggatgaacat ggattttatc gcattacggg cagggtagat gatgtgttga atgtgagtgg
     4861 gcatcgtata ggaactgcag aagttgaaaa tgccataaat aggtatattg atgtggtgga
     4921 aagtgctgta gtgggctatc cgcatgacat aaaagggcag gggatatatg cattcgttat
     4981 tttgcaaaat gaattacatg atgccgaatt aaccaagcaa gatattatac aaaccgtaaa
     5041 ccgaataatc ggacctattg ccaaaccgga taaaatacaa tttgtcattg ggcttcctaa
     5101 gactcgcagc ggaaaaataa tgcgtcgaat tttgagaaaa attgcagaag gagaaacttc
     5161 caatttgggt gatacttcaa cattactcga tcctagtgtg gtagatgaga taaaaaatgg
     5221 tgctatctaa ttcccgaatt ctcaattaga agtgccgaat tcttgtttta tttgctaaat
     5281 gatgtaagat gaaataactt catccaaaat catagtatga aaatcgaaat atttgaattt
     5341 ccaccggaaa gtgaagcttt aaaaaataag aaaacacatt ctaaaaatga tttgactgca
     5401 cagcaaactg attccaataa gtcgaatcat aaaaagagga atacagaaaa aatcaaatct
     5461 gaaaagaata gtaagcagag ttcgggttaa gaaaaataag gtaaagaata gttgatttct
     5521 caattttctt ccaacttaac aaagaggagg aataataagg tggtggtgtt taataaaaaa
     5581 atcaactatt ttaaattgaa atgcttatcc taaatacagg ttaataaaca gcaaaaagaa
     5641 tggaattact tgtattgcat aatatctaat tgcaaagaac agtcttcata ttcataagcc
     5701 tggttggagc cgattattag tgttctttta ccgacatatt tttctttcat gcttaaatac
     5761 cagtcgacag ctttggtgtc aaaattagaa gtaggttcat ccaataagat aagttcagat
     5821 tgtgtgagaa aggcaaacgt caacttaaga cgttgtcgca tgccggatga aaaattttta
     5881 atatacttgg tcttatgttt ttccagttcg ctgatggcat aaaattcagc ttcttcgctc
     5941 gtataaaatg ttttgaaagc cgcatgaaat ttatacattt cttccagtgt aaaatcttca
     6001 atcaatgaga tataaggtgc agcataaaca attttctgaa ctgcaatttc ttcaagctca
     6061 ccaaaatcca ctttaccgga tgtaggcgta agtccacctg aaataatttt cagcaatgta
     6121 gattttccac ttccatttgg accaactaca gcatacttgc ctccctcttc aaagctaaat
     6181 tgaatttgct taaaaatcca ttcaaagcca aagcgtttac tgatattttg taaagtgatg
     6241 gtcaagttgt gattagttat tggtgattag ttattggtga ttagttattg gttatttgtg
     6301 atctgttata agttgtaagt tattggagct aataaaaaat catcgtttat catttatcat
     6361 tcaacattca acattcaaca ttcaacattc atttaatcca ttcctttaat aattcctcgt
     6421 tctgaagttt tcacaaaatc aatgatataa tttctaatgt ctgaaggctg gatttcttct
     6481 tctatttttt gtagggcttg tgtcgtatta agtccgttat tgctaaatat aatacgatag
     6541 atatcttgaa tctgatgaat gtcgtctctg gaaaattcgg ctctgcgcag accaacagaa
     6601 ttgataccgg cgtactgggc tggatttcgc atcactaaac aataaggagg aacatctttg
     6661 cctatcatac tgcctccgga aataaagctg tacttgccta tacgcgtaaa ttgatgtgct
     6721 ccgctcaacc caccgaaata ggcataatca tctatcgtta cgtgtccggc aatattcgtc
     6781 acatttgcca gaatacaatg attgccaata gtacaatcgt gggcaatatg tacataggcc
     6841 attaataaac tattactgcc aatgctcgtt ttgcctaatg cagaagtgcc tctgttaata
     6901 gtaacacatt cgcgaattgt tgtacaatca ccgatttcaa ctgtcgtata ttcaccggca
     6961 aattttaaat cctgtggaac ggcaccaaga actgcacccg gaaaaattct gcagttttta
     7021 cctattcttg ttcccggaaa aatcgtaaca tttggaccaa tccaggtgtt gtctcctatg
     7081 ctgacatcat cgtaaattgt agagaaatgt tctacctgta cattcgtacc taaatgtgca
     7141 tttttatgga taaaattggt agccatatta agtggcaaag ataatcaatt aaaaacggct
     7201 tgtttgaaaa ttatacagtt accgaagtat ggatatattt atcaaccaga aattgggcaa
     7261 attttacatt agaggtatgt cccggtttgt aacagattaa tttgccttcg aagtaggttt
     7321 gtgtgagggc taaatctcca atcacatcca atattttatg tctggctgct tcattgtcat
     7381 acgactgaaa caggggatta agtacaccca tttcaggtat ggaattagct tgctgatgaa
     7441 aagtttttgc cagccagtta gctttttcat ttgaaatagt tgattcagaa aatacaagtg
     7501 cattagtcaa gttgccgcct tttatcaagc ctttattata taaaaattct atttcgtgca
     7561 aaaaacaaaa tgttttagca ttggcaattt cagaagtata ttccgaccag tggttcatca
     7621 cggcatactg atgtttaatt agtttggaag ggaagtcaat taagcaggtt accgaaaagt
     7681 cttcatgagg cagcaatata tactcagctc cggtatcttc atctgtccat tccaccacat
     7741 cggggatttt ttgaaaacgc tttggttcat tcagttcgat aatattatca tcgtttagat
     7801 ttttgataaa acaggagcaa cttccatcta aaatgggcag ttccagattg tcaatttcaa
     7861 ttaatgcatt atctatccct tttgcataca aagcagatag taagtgttct acggtaatca
     7921 catctaccag ttcattcgtg agtgttgtac tcctgtttgt atttgtaaca tatttacttt
     7981 gagccggtat cattgcacgg tctaaatctg ttctgcaaaa tacaattcca tgattttccg
     8041 gtgccggatg caccgttact gtagaaaata cacctgtatg caagccgtta cctgaaaatg
     8101 aaaatgactt gacgatcgtt ttttgtaaaa tcactcgtta cttttaaatt ttaaggtcaa
     8161 tcaaagatag taataaaact gcattctcaa aaatgataaa cgataaaata cagatattga
     8221 taaaaataat aatacatgaa aatagatgca ttattttctg acagattttg cttcttcgag
     8281 cataaatttt tctgcagtgt ct
//
LOCUS       DAOQII010000097         8051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4851_length_8051_cov_4.138319, whole genome shotgun sequence.
ACCESSION   DAOQII010000097 DAOQII010000000
VERSION     DAOQII010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4851_length_8051_cov_4.138319"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="PLF48_06060"
     CDS             <1..2052
                     /locus_tag="PLF48_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF48926.1"
                     /translation="KDGLPLKGLSNKDFYKLNAYRFSKKAIPISNNFYYLAFKENGEK
                     YLGFFPMREDTLQLGYLFVEFTPKIFSSFSAYPELLMKQNNYFDEEFDNYAYGIYDQK
                     HLVKQKGDYEYKLDFNYQQKGHAEYGNFETANYNHLVYFGDEKQVVLSEQSRPALSTI
                     SIFSYMIIFFLLFFIFVDAIGFSDRFWGNESIRNFMRNNTLQKQIQNAMIALVLVSLV
                     IIGIVTLVYFQYQYNLYHNSRLLKKVNTVMKNVSQYYVDEYPQYKSQTFNVISNKYIK
                     SLSNIHALDINVYDNKGELLYSTQPEIFKRQLLSNKMDADAYYNLVVKNKSKYVHDES
                     IGKLKFLSAYQPFRSNGKVLGYYNFPYYGKEKSFREDISYFLVALVNVYVIFLIAAAL
                     LAIFLSRSITNSLKLISDNIKNVQFGKSNQPIFWKNQDEIGLLVKQYNLMLAELEKSA
                     GLLAKSEREGAWREMAKQVAHEIKNPLTPMKLSIQHLQRAINDNSPDIQELTKRVSER
                     LIEQIDNLSNIASAFSDFAKMPVGNFEKIDIVPLIKSTVELFGQHEEISFEVQYPNEA
                     YEVLGDKNQIIRVFTNIIKNSVQAIPENTEGKIKIDFSKTEKDCLISIFDNGTGIPED
                     KRDKIFEPNFTTKSSGTGLGLAICKNTIERMEGKIWFVSKEGEYTIFYLEFPLFNNDK
                     K"
     gene            2081..2154
                     /locus_tag="PLF48_06065"
     tRNA            2081..2154
                     /locus_tag="PLF48_06065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ala,seq:ggc)
     gene            2335..3975
                     /gene="ade"
                     /locus_tag="PLF48_06070"
     CDS             2335..3975
                     /gene="ade"
                     /locus_tag="PLF48_06070"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNF48927.1"
                     /translation="MLDSTTILANYVDIQHREIFPAEIIIQGGRIHAVKKINKILNQF
                     ILPGFVDAHIHIESSMLVPTEFAKQAVIHGTVATVSDPHEIANVLGKTGVYYMLDNAK
                     NSPLKFNFGAPSCVPATTFETAGAELSADDIDELMALNEIKYLAEMMNYPGVLFRDKM
                     VMDKIAVAKKHHKPVDGHAPGLIGEKAVQYINAGISTDHECFTYDEAKFKLEHGMKVL
                     IREGSAAKNFDALIDLLHEYPDEMMFCSDDKHPDDLLLGHINLLAKRAIAKGIDVFNV
                     LQAACFNPVLHYKLEVGLLKENDPADFIIVNNLTDFDIMETYIDGHLVAKEGVSYINT
                     TPEKCINNFSTHTKLPSDFELELTSDKIKVIEVLDGQLITKTYIGHIKLDEQGHAFSN
                     LEDDILKIAVINRYYDAPVAIAFIKNFGLKTGAIASSVAHDSHNIIVVGVDDESMCYA
                     ANAIIKEQGGLSIADGMNTHILPLPVAGLMNTESCKFVAEKYTHLVSTAKEMGCTLHS
                     PFMSLSFMALLVIPQLKLSDKGLFDGSTFEFCDLSADA"
     gene            4262..5881
                     /locus_tag="PLF48_06075"
     CDS             4262..5881
                     /locus_tag="PLF48_06075"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF48928.1"
                     /translation="MPLDSIAQLELQLKETTQLSTEYVDLLHQLVFHYLNSDLDKAKS
                     LIEEALHIANEIDYKDGIGMSLHQKGVTFYNIGDFENALNYYMEADKTLADSKDWKKS
                     IKPKANIGMVYIHTSQYEEALRIYKEIELELEQVPLDIIHAQMYINIDVAYCLLKDPE
                     NGIIYSKKALEIAHQLNNLYGVAISHTNLAGHLILLNKPEEAIIHLTKSEKICLKENY
                     KIIQTGNYLKFADCYTRLKDTDKALEYIALGISSAQETMDMEKEALLLKQKMIIYEIQ
                     QLFELAYNTSKSYLEKKEAFLNAERIKSFNTLQLRYETEKKEVALNQLKLEQVQSELT
                     ALKSQMNPHFIFNVLNSIQELYTLGDKHQANIQMGNFARLTRKILEVSGKQVIDMDEE
                     IEILTTYLNLESMRFETDFSYTIEIPENAEELYIPPMLIQPFIENSIKHGLLHKKGEK
                     KLEISFEINANDDILICRIADNGIGRKASEEINKQRHKLHQSFAASATEKRLQLLNMG
                     KTKNIAVSYEDLTDSLGTAAGTSVTLCIPYS"
     gene            6027..6785
                     /locus_tag="PLF48_06080"
     CDS             6027..6785
                     /locus_tag="PLF48_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF48929.1"
                     /translation="MLRTIIIDDEAKARRMLTTLLKENCPQVELVAEVADVPAGVKAI
                     QQFQPHLVFSDIDMPGYNGFQLLDFVDKANFELIYCTGHDEFALKAFEVSAIDYLLKP
                     VQITQLVNAVQKAEKLCLSSAGQQAQRRHTLKENLSEDVLTKMALPVTDGLIFVNIED
                     IIFLEAEGAYTYVNLKDGNKLIICKKLKEFENMLSNNKNFFRIHRSFLVNTTFIKQYL
                     KSDGGSVLLQNNITVPVARERKDDFQRLVEFIKL"
     gene            complement(6803..7126)
                     /locus_tag="PLF48_06085"
     CDS             complement(6803..7126)
                     /locus_tag="PLF48_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNF48930.1"
                     /translation="MTRLETHYITAGNTYLGIIDGNTSTTLDDFYKEIKTALQFPNYF
                     GNNLDALDECLNDLSGIEEENIVLLIINYAGFLSEEASKKQIVADIIANAEAENKYFS
                     VYYIY"
     gene            complement(7123..7611)
                     /locus_tag="PLF48_06090"
     CDS             complement(7123..7611)
                     /locus_tag="PLF48_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNF48931.1"
                     /translation="MKKFVYLFLSFLFFACNSNQQDIQKAYQNGYNDGYTAASSHHEQ
                     STSPVFSQQPQSNVVDNPSETNENAAGVLTIPQKVYTTLNYIKQYHSAPDGYVGGRKF
                     GNYENLLPKKDAAGNTISYQEWDVNPKIEGQNRGAQRLVTGSDGRAWYTANHYKSFTE
                     VK"
     gene            complement(7615..>8051)
                     /locus_tag="PLF48_06095"
     CDS             complement(7615..>8051)
                     /locus_tag="PLF48_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNF48932.1"
                     /translation="KLAALHKNIVVKVPMIKDGIKAIRYFSDNGIKTNCTLVFSPGQA
                     LLAAKAGATYLSPFIGRIDDMNVDGMELIYQIRRIYDNYNFETQILAASIRNALHIVK
                     CAEIGADVVTCPLSSILGLFNHPLTEKGLAQFLADHAKANAK"
BASE COUNT         2735 a         1393 c         1421 g         2502 t
ORIGIN      
        1 aaagacggtc ttccgctaaa aggattgagt aataaagatt tctacaagct caatgcttat
       61 cgcttttcta aaaaagcaat acctatttct aataattttt attatctcgc ttttaaagaa
      121 aacggagaaa aatacctggg ctttttccct atgagagaag acacactgca actgggttat
      181 ttatttgtag aatttacgcc taaaatattt tcatctttca gtgcttatcc ggaattactg
      241 atgaagcaga ataattattt tgatgaagaa tttgacaatt atgcctatgg catttatgac
      301 cagaagcacc ttgtaaaaca aaaaggagat tatgagtaca aactcgattt taattatcaa
      361 caaaaaggac atgcagaata tggcaatttt gaaactgcca attacaatca tttagtgtat
      421 tttggagatg agaaacaagt ggtactttcc gaacaaagcc gtccggcact gagtaccatt
      481 tccatttttt cttacatgat tattttcttt ttgctcttct tcatctttgt tgatgccata
      541 ggattttccg atcgtttttg gggaaatgaa tctatacgaa attttatgcg caataatacc
      601 ctgcaaaagc aaatacagaa tgctatgatt gcattggtgc ttgtttcatt ggtcattatc
      661 ggtattgtta cgcttgtata ttttcaatac cagtataatt tataccacaa cagccgttta
      721 ttgaaaaaag taaatacggt gatgaaaaat gtatcgcagt attatgtcga cgaatatccg
      781 caatacaaaa gccagacatt taatgtcatt tccaataagt atatcaaatc actttccaat
      841 attcatgcac tcgacatcaa tgtttatgac aataaagggg aactgctcta ttctacacaa
      901 cctgaaattt tcaaacgcca gctattatca aacaaaatgg atgcagatgc ctattacaat
      961 ttagtagtta aaaataaatc gaaatatgtt catgatgaat ccattggaaa acttaagttt
     1021 ttaagtgctt accagccgtt tcgcagtaat ggcaaagtgc tgggttatta taatttccca
     1081 tattatggaa aagaaaaatc attccgcgaa gatatctcct atttcttagt agctttagtc
     1141 aatgtgtatg ttatcttttt gattgcagcg gctctgctgg caatattttt atctcgttct
     1201 attaccaatt cgcttaagct tatttcagac aatatcaaaa atgtacagtt tggcaaatca
     1261 aaccaaccaa tattctggaa aaatcaggat gaaatcggac ttttagtaaa acaatacaat
     1321 ctgatgttgg cagagttgga aaaaagtgcc ggactattag ccaaatcaga acgtgaaggt
     1381 gcatggcggg aaatggcaaa acaggtagca cacgaaatta agaacccgct tacgcctatg
     1441 aaacttagca tacagcactt gcaaagggct ataaatgaca attctccaga catacaagaa
     1501 cttactaaaa gagtttctga aagattaatt gaacagattg acaacttatc aaatatagct
     1561 tccgcattct ctgattttgc aaagatgcca gtgggaaatt ttgaaaaaat agatatcgtt
     1621 ccgctaataa aatctacggt agaattattc ggacagcacg aagaaatttc ttttgaagtg
     1681 caatatccaa atgaagctta tgaagtgctt ggtgataaaa accaaatcat acgtgtattc
     1741 accaatatta tcaaaaactc agtgcaggca attccggaaa acactgaagg aaaaataaaa
     1801 atagattttt caaaaacaga aaaagattgt ttgatttcca ttttcgacaa tggcacaggg
     1861 attcccgaag ataaacgcga taaaattttc gagcctaatt ttacaaccaa atcttcgggt
     1921 accggacttg gcttggccat ttgcaaaaac acgatagagc gcatggaagg taaaatttgg
     1981 tttgtttcga aagaaggaga atacacgata ttttatttgg aatttccgct gtttaataat
     2041 gacaaaaaat gattgaaaaa atgtacattt gcagcacaat ggggcattag ctcagatggc
     2101 tagagtgctt gactggcagt caagaggtca tcggttcgat tccgatatgc tccacttcat
     2161 tttactacct acctttatta tgctgaactt gtttggcata tttttttgtc atcatctgaa
     2221 aaaacttgaa ttatagaagt tgcttctttt acaaaaaaat gcaggaaaga aaatggcttg
     2281 tatcgtttgg catttaattt tactaggttt tcataaagtt tcttaaattt agaaatgctt
     2341 gactctacta ctattttagc aaactatgtt gacattcaac atcgcgaaat ttttccggca
     2401 gaaattataa tacagggagg acgtattcat gctgtaaaaa aaattaacaa aatacttaac
     2461 caatttattt tacccggttt tgttgatgca catattcata ttgaaagctc tatgctggta
     2521 cctactgaat ttgcgaaaca agccgtcata catggtactg tagctaccgt ttctgatcca
     2581 cacgaaattg ccaatgtact tggaaaaaca ggtgtatact acatgttgga taatgcaaaa
     2641 aattcacctt tgaaatttaa ttttggtgct ccttcctgtg tgccggccac tacttttgaa
     2701 acagcaggag ctgaattatc agctgatgat attgacgaac taatggcatt aaatgaaata
     2761 aaatatctgg cagaaatgat gaactatccc ggtgttttgt ttcgggataa aatggtaatg
     2821 gataaaatcg cagttgcaaa aaaacaccat aaacctgttg acgggcatgc tccgggatta
     2881 attggcgaaa aggctgttca atatataaat gccggcattt ctacagacca tgaatgtttc
     2941 acttatgatg aagctaaatt caaattagaa cacggcatga aagtcctgat tcgggaagga
     3001 agtgcggcaa aaaattttga tgccctgatt gatttgttac atgaatatcc agatgaaatg
     3061 atgttttgtt ctgatgacaa gcatccggat gatttattgc tcgggcatat caatttgctg
     3121 gcaaaacgtg caatagcaaa aggaatcgat gtttttaatg tgttacaagc cgcttgcttt
     3181 aatcctgttt tacattacaa attagaagtt ggattattaa aagaaaatga ccctgcagat
     3241 ttcatcatcg ttaacaactt gactgatttt gatattatgg aaacatatat tgatggtcat
     3301 ttagtagcaa aagagggggt cagctatatt aatacaacgc cagaaaaatg tataaacaat
     3361 ttttcaacgc atactaagtt gccttcagac tttgaattag aactgaccag tgataaaata
     3421 aaagtaattg aagtattaga tggtcagctg attacaaaaa cttatatcgg ccatataaaa
     3481 ctagatgaac aagggcatgc tttctctaat cttgaagatg atattttgaa aatagcggtt
     3541 atcaatcgtt attatgatgc tcctgttgca attgccttta taaaaaattt tggtttaaag
     3601 accggtgcaa ttgcttcatc agtagctcat gattcacata atatcatcgt ggttggggtt
     3661 gatgatgaaa gtatgtgtta tgctgcaaat gcaataataa aggagcaggg tggtctatca
     3721 attgcagatg gaatgaatac ccatattttg ccattgcctg ttgcaggatt aatgaatacg
     3781 gagagttgta aatttgtagc ggaaaaatat actcatttgg tcagtactgc aaaagaaatg
     3841 ggctgtactt tacattcgcc atttatgtcc ttatccttta tggcattatt agtaatacca
     3901 caattaaaac tgagtgacaa gggcttattt gatggctcta cttttgaatt ctgtgatttg
     3961 agtgcagatg cttgaccttg ctttctaaat ataagatgta atgaaataac atgtgcatca
     4021 aaatttatat tttccgaatg tactatctca aatcgtactg attgataata ttttagtttt
     4081 aaaaagatat tttctggagt aagcctaaat aatactcaga caaaagacat taaatatttg
     4141 gatatgtaac caaaatccaa gcggtatttt actgtgtttt tataaaaaaa ctaaaggtaa
     4201 aaaacccatg aatttatatc tttacgtcat tcctggttta tcaacataaa actactctcc
     4261 catgcctctc gacagcatcg cccaacttga acttcaatta aaagaaacta cacaactctc
     4321 aacggagtac gtagatttat tgcaccaact cgtttttcac tatttaaatt cagatttaga
     4381 taaagccaag tcactgattg aagaggctct ccatattgcc aatgaaattg attacaaaga
     4441 cggaattggt atgtctttgc atcaaaaagg agtcactttt tataacatcg gcgattttga
     4501 aaatgcgctg aattattaca tggaagcaga taaaacatta gcggatagta aagactggaa
     4561 aaaaagtata aaaccgaaag ccaatatcgg catggtctac atccacacga gtcagtatga
     4621 agaagctctt cgtatttata aagagattga acttgaactg gagcaagtac ccttagacat
     4681 cattcatgca caaatgtata tcaatattga tgtggcttat tgtttattaa aagatccaga
     4741 aaatgggatt atttattcga agaaagcatt ggaaattgcc catcagctga ataatttata
     4801 tggagttgct atcagtcata ccaatttggc agggcatctc atcttattaa acaaacccga
     4861 agaagctatt atacatttaa caaaatcaga aaaaatctgt ctgaaagaaa actataaaat
     4921 tatacaaacg ggcaattatt tgaagtttgc agattgctac acacgactaa aggatacgga
     4981 caaggcattg gaatatatcg cacttggcat aagctctgca caggaaacaa tggacatgga
     5041 aaaagaagca ttattactca aacaaaaaat gattatttat gaaatacaac aattgtttga
     5101 attagcttat aatacctcaa aatcatatct ggaaaagaaa gaagcatttt tgaatgccga
     5161 acgcatcaaa tcgttcaata cattacaact cagatacgaa acagaaaaga aagaggtagc
     5221 actcaaccaa ctaaaactag aacaggtgca aagtgaactg acggcattaa agtcgcaaat
     5281 gaacccgcac tttattttca atgtacttaa ctcaatacaa gaattataca ccttgggcga
     5341 caaacatcaa gcaaatatac aaatgggaaa ctttgctcgt ttaacccgca aaattcttga
     5401 agtttcaggc aagcaagtga tagacatgga tgaagaaata gaaatactga ctacttatct
     5461 gaacttagaa agtatgcgct ttgaaactga tttttcatac actattgaga ttcctgaaaa
     5521 tgccgaagaa ctttacattc cacccatgct catacaaccc tttattgaaa acagtataaa
     5581 acatggactg cttcacaaga aaggtgaaaa aaagttagaa ataagttttg agataaatgc
     5641 caatgatgac atattaattt gccgtattgc cgataatgga attgggcgaa aagcttctga
     5701 agaaataaat aaacaacgcc ataagttgca tcagtccttt gcagcttctg ccacagaaaa
     5761 aagattacaa cttttaaata tgggaaaaac taaaaatatt gctgtaagct atgaagatct
     5821 taccgatagc ttgggtactg ctgcaggcac ctcggtaacg ctttgcattc cgtattccta
     5881 aacaaaattt taccccattt ttctttttcc taacttagca tattatctgt attgtcaatt
     5941 tttcaacttc gttgttttat tcacgcataa ctgataaaat acaaaaaaat tgaaatatat
     6001 tagcattcca aggaagcagc tatatcatgt taagaaccat aattatcgac gatgaagcga
     6061 aagccagaag aatgctgact actttactca aagaaaactg tcctcaggtg gagctggtgg
     6121 cagaggttgc cgacgtgcct gcgggtgtga aagctattca gcaatttcag cctcatcttg
     6181 tattttctga tattgatatg ccgggctata atggatttca attgcttgat tttgtagata
     6241 aagcaaattt tgaactcatt tattgcaccg gacacgatga atttgctcta aaggcgtttg
     6301 aagtttctgc tatcgattat ttactaaaac ctgttcaaat tacacaactt gtcaatgcag
     6361 tacaaaaagc tgaaaagcta tgtctttctt cggcaggcca acaagcacaa aggaggcata
     6421 ccttaaagga aaatctaagt gaagacgtgc tcaccaaaat ggctttgcca gtaactgatg
     6481 gactcatttt tgtcaatatt gaagacatca tttttttaga ggctgagggt gcatatacgt
     6541 atgtaaacct taaagacggc aataaactta ttatttgcaa aaaactgaaa gaatttgaaa
     6601 atatgctttc aaacaacaaa aattttttcc ggattcaccg ctcatttctt gtcaatacga
     6661 ctttcataaa acaatatctg aaatcagacg gtggcagtgt gttacttcaa aacaatataa
     6721 ccgtacccgt agctcgtgaa agaaaagacg attttcagcg tctggtagaa tttattaaat
     6781 tgtagtaaat ttatatatta tttcaataaa tataatatac actgaaatac ttattttctg
     6841 cctcggcatt tgctataata tctgctacta tttgtttttt agatgcttct tcagataaaa
     6901 aaccggcata attaataatc agcaatacga tattttcttc ttcaatgcca cttaaatcat
     6961 tcagacattc atccaacgca tccagattat ttccaaaata attcggaaat tgcaaggctg
     7021 tttttatctc tttgtaaaaa tcatctaaag tggtactggt atttccgtct ataataccta
     7081 aataagtatt tccagccgta atatagtgtg tttctaatct ggtcatttta cttcagtaaa
     7141 tgatttataa tgatttgcag tgtaccatgc cctgccgtcg ctgccggtaa ctaatcgctg
     7201 cgcacctctg ttttgtcctt ctattttcgg gttaacgtcc cattcctggt aagaaatagt
     7261 attgcctgct gcatcttttt taggtaagag attttcataa ttaccaaatt tacgtcctcc
     7321 aacataacca tcaggagctg agtggtattg cttgatataa tttagggtag tgtatacttt
     7381 ttggggaatc gttagtactc ctgcagcatt ttcatttgtt tcagatggat tatctacaac
     7441 atttgactgg ggctgttgac tgaagacagg cgaagttgat tgttcatgat gagatgatgc
     7501 cgcagtatag ccatcattgt agccgttttg atatgctttt tgtatgtcct gttgattgct
     7561 gttacaagca aaaaacagga atgaaaggaa aaggtaaacg aactttttca ttttttattt
     7621 agcatttgct ttagcatggt cagccagaaa ctgggctaaa cccttctctg taagtggatg
     7681 attgaacaac cctaaaatgg agctaagcgg gcaagtaacc acatctgcgc caatttccgc
     7741 acatttcact atatgcaatg catttcgaat ggaagctgcc agaatttgtg tttcaaaatt
     7801 ataattatca taaatacggc gaatctgata aatcaattcc ataccatcta cattcatatc
     7861 atcaattctg ccaataaacg ggcttaaata ggtagctccg gcttttgctg ccaacaaggc
     7921 ctgccccgga ctaaatacca aggtacaatt agtttttatg ccattatctg aaaaataccg
     7981 gattgctttt atgccatctt ttatcatggg cactttcact acaatatttt tgtgcaatgc
     8041 cgccagtttt t
//
LOCUS       DAOQII010000098         8000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4889_length_8000_cov_4.630082, whole genome shotgun sequence.
ACCESSION   DAOQII010000098 DAOQII010000000
VERSION     DAOQII010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4889_length_8000_cov_4.630082"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..796)
                     /locus_tag="PLF48_06100"
     CDS             complement(32..796)
                     /locus_tag="PLF48_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48933.1"
                     /translation="METRINYSHYESEILNLKVGRCVTEQLDINSFNKQLTEEAYDLI
                     RLTVASDDEFIIDKLYSAGIPYFFAAGITRYKTAIERIPVAPYINEGIEFVEFDGTQT
                     QLLYDMIKATLGNYPIGYFRTPILKEIISKEMEIEVLYEFYKFNNLKTNNINNSMMFI
                     KHQENFVGYFALNILPDRLESHIGGIREPFRTNGYFVDMQEFIKRYCIQNQLTYFCFG
                     ARNENSRAQHIFQKYGYQRESMEYVFHLPLFLSTKK"
     gene            847..1713
                     /locus_tag="PLF48_06105"
     CDS             847..1713
                     /locus_tag="PLF48_06105"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HNF48934.1"
                     /translation="MSAGYVHSNLKDLSTIHTYRFLISIAIEYLVIFLSIGIAFYTHY
                     TIFFCVITVCVIGTRQYALFSLLHEALHYNISRKKKFNDTVGKLFLSIPLFIDFDKMR
                     KVHLMHHKHLHTQQDPELSILQYKSFHFPKTKKQLVVLFLGDLLGINYSIIFAKTFLN
                     KSLKSKLLFTVLSALLIFLLFKMNILQFVIVFWILPYITIFQFLNRLRLATEHFNIPD
                     AKFKTRSMHTNWIENLIFNPYCIGYHHEHHNYPAVPYYNLRKLYLSVKDIESKNMYIE
                     KSYLTLFVKLLK"
     gene            1710..2501
                     /locus_tag="PLF48_06110"
     CDS             1710..2501
                     /locus_tag="PLF48_06110"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF48935.1"
                     /translation="MTPTVYDITTIDQQTFTSKFEEDGFVLVNNIFPEELLDDINSVI
                     LGCVNHEADTYHHRKHKDYGMLLCCPVYGAPFTTILDHESFFKPFNWLLGDNCIVWVY
                     TSSCIAPKQTNFASRIHLERTIFTGDFIDGVGSLILLDDFNESNGATYLLKGSHKNLE
                     KPEDERFYKEAIRLVAPKGTVLYFNLRLWHAGGQNHTVKWRNSLGIGVNRPYLKQRID
                     IPRILPQAVKDKLSDYAKNKLGFNVQVPSSFEEYYRNEKKSYTNK"
     gene            complement(2518..3852)
                     /locus_tag="PLF48_06115"
     CDS             complement(2518..3852)
                     /locus_tag="PLF48_06115"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNF48936.1"
                     /translation="MQKSYNRSEVLQHNTLDDCWVIINNKVYDLSDFIKKHPGGKEII
                     ETRAGEDATSFFYLKHVSNKGILRQLETYCIGEICASEKIDLEALKEPFLNELLESVM
                     KQNLYTVSKKELRSFNLIRFFNLLVFFVCYALVFYTQLAIIYKSLLIILQAMVGASLF
                     GFLAHEATHRNYPKTRLSKIVLSIYWPLCWPFISEKALRYEHNSHHIKIGDTAYDFEV
                     AGFSKFIRYSNNITPNFLHRYQHKLAMFFYPFYANIITTYGGISSDFWNIHNRKVAWL
                     HNLSILFSLFYFILLPAFCTTISWYFLVIFYLCYQCILFSLVYVSAAINHFIPETVKP
                     LPEAYKNYPGYYVCHNTSNFCADSNYWFWQSGGFNIQIEHHLLPFIPVENLHKLKKIV
                     KELCHKYQYPYYEFDSFGQMWKEHYSFLKMLSKYDETLWQTDEMNKRNYEAK"
     gene            complement(3969..5498)
                     /locus_tag="PLF48_06120"
     CDS             complement(3969..5498)
                     /locus_tag="PLF48_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48937.1"
                     /translation="MIILHHFEMQQEIGEKRWETVLIVMVILFLLTVRLLLIFSYSVD
                     LDGMESAFVIYVQRLTDNQPLYGNPTDYPYFVCLFLPTYIYVLKFLVEIFGAGSKTDP
                     HVIFIIGRAFSFSVFLLQVCLSFFYCKKRFLISNRNLLFGVMLYLLLISGHMYVMRPD
                     ALKVLFFNTAILFLVEYTFFTKKNWILLSAVFFFLLSTVVKQDMLIYSSLILLATFLF
                     RKDYKYFLSWMCYMLLLVVWLSCYRLYFGAYFFDHVVLFNFQKLTKVYSSFNLFVVLL
                     SLSRLMPIFLFLFFYKTEKRSISVLLKIWTAVFLVVSHFFMLRAGSYLNYGYETVFLL
                     VFALLVLYSNERKKLYLINYKLFTCFFLLYLFATNYIIHSYNYSFKEQEILKTSYMQK
                     VKEVQQIHEMIQDKPIFVTDLRNVFFYHHCNLIYGYDYNITRFMEVYLPLKVYKLLPK
                     GVYSYPVFFNPSTYNKKFLDGSVPYIASPIDRDFFIKKYYSLYQPADTIGSVILYKYK
                     N"
     gene            5554..7104
                     /locus_tag="PLF48_06125"
     CDS             5554..7104
                     /locus_tag="PLF48_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF48938.1"
                     /translation="MKSLYFTEEHELFRDSVRKFVEKEIVPNANQWEKDKKIPRDLFL
                     KLGEQGFLGINHSEKFGGSDADIFYTCAYLEEMAKSTYAGVCAAVSVHQYMATNHLAE
                     AGSKELKERFLRPSIEGKMVGAIAITEPFGGSDVQAMRTTAIKDGDYYIINGSKTFIT
                     NGHFCDFVVVACKTDNNAGINGISLIVVERGTPGFTSTQLEKIGWHSSDTGELAFDNV
                     KVPVNNLVGKEGMGFFYIMESFQVERLVAGILGIGGGEVCLDATLNYMNEREAFGRQI
                     KKFQVLRHDMVQLYTELEAGKQMTYHACWLLQNGEVPVKECTMVKLYMTELGNKIVDK
                     CLQMFGGYGYMEDFAIARAYRDARVGTIVGGTTQIMREILSKIIIDDVRYKKVYNETN
                     ELTNTPTENKEKSWGHPLTAKEIIHSIPLRIKHDKAAAYSTVFHFDISGENGGQFTVK
                     IENGNAVVSDDFEGTPDCVVTASDTVYADIELGKLDPTMAFMGGQIKVSNIGAMMQFS
                     KYFHRITS"
     gene            7250..7780
                     /gene="pyrR"
                     /locus_tag="PLF48_06130"
     CDS             7250..7780
                     /gene="pyrR"
                     /locus_tag="PLF48_06130"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNF48939.1"
                     /translation="MSSEILLQHRQIQLIIERLSRQIIESLDLRNTVIIGVQPRGTLF
                     AEKIHHNIEQLSKLKIPFGKIDTTFHRDDFRRGKNLNAKVTEIDFLLEGKTVLLLDDV
                     LYTGRTIRAAMDAMLTFGRPEKVELMVLIDRRFNRELPIQADFIGKTVDTIEHINVEV
                     IWDSANADNKVIFINE"
     gene            7785..>8000
                     /locus_tag="PLF48_06135"
     CDS             7785..>8000
                     /locus_tag="PLF48_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNF48940.1"
                     /translation="MSQLSSKHLIGIEHLTKEDLELILTTAVEFKDIINRPIKKVPTL
                     RDITIANLFFENSTRTRISFELAQKRLS"
BASE COUNT         2769 a         1391 c         1356 g         2484 t
ORIGIN      
        1 aatagtcgag tcatttgatt aatattcagt cttatttttt cgtactgaga aaaagaggca
       61 aatgaaaaac gtactccatg ctttctcgct gatagccgta tttctgaaaa atatgctgtg
      121 ctctgctgtt ttcatttctg gctccgaaac aaaaataagt caattgattt tggatgcaat
      181 atcgtttaat aaattcctgc atatccacaa aataaccatt tgttcgaaag ggctctctaa
      241 taccgccgat atgtgattct aatctatctg gcaaaatatt taaagcaaaa taacccacaa
      301 aattttcttg atgtttaata aacatcatcg agttgtttat gttgtttgtt tttagattat
      361 tgaatttata aaactcataa agtacctcaa tctccatctc tttacttatt atttctttta
      421 aaataggtgt cctgaaataa cctatcggat aattgcctaa agttgctttt atcatatcat
      481 aaagcagttg tgtttgtgtg ccgtcaaatt caacaaactc aattccttcg ttgatataag
      541 gtgcaacagg aatacgctct atggctgttt tgtaacgtgt aataccggca gcaaaaaaat
      601 aaggaatacc tgctgaatat aatttatcta tgataaattc atcatcagaa gcaaccgtta
      661 atcgaattaa atcatacgct tcctctgtca gctgcttatt aaaactgtta atatccagtt
      721 gttctgtaac acatctgccg acttttaaat ttaaaatttc tgattcgtag tgagagtaat
      781 taatccttgt ttccattgga ataaaattat aaatttacat cacaaattaa tgttatcttt
      841 ctatttatga gcgcaggata tgttcattct aatcttaagg atttaagcac catccataca
      901 tatcggtttt tgatttccat agccatagaa tatcttgtca tttttctttc gattggaatt
      961 gcattttata cccattatac tattttcttt tgtgtcatta cagtatgtgt aatcggtacc
     1021 cggcagtatg cattattcag tttgctccac gaggcactgc attataatat ttccaggaaa
     1081 aaaaaattta atgacacagt aggcaagctc tttttatcga ttccattatt tattgatttt
     1141 gataagatgc gcaaagtaca tctgatgcat cataaacatt tgcatacaca acaagatccg
     1201 gaactttcca ttttacaata taaatctttt catttcccaa aaacaaaaaa acaacttgtc
     1261 gtattatttt tgggggatct tttgggtatc aattacagta tcatttttgc taaaacattt
     1321 ttaaataaaa gcttaaaatc caaactccta tttacagtat taagtgcatt actgattttc
     1381 ttgttattta aaatgaatat tctacaattt gttattgttt tctggatact tccctatatt
     1441 actatatttc aatttttaaa cagattacgc ttagccacag agcactttaa tattcctgat
     1501 gcaaaattca aaacaagaag tatgcatacc aactggatag aaaatctgat ttttaatccc
     1561 tactgtatcg gctatcacca cgaacatcat aattatccgg ctgtgcctta ttataactta
     1621 cgcaaattat acctcagtgt caaggatatt gaatctaaaa atatgtatat agagaaaagc
     1681 tacttaacac tctttgtaaa acttttgaaa tgactccaac agtatatgat attacaacta
     1741 ttgaccagca aactttcaca tcaaaatttg aagaagatgg atttgttttg gtgaataata
     1801 ttttcccgga agaattatta gacgatataa actccgttat tttaggttgt gtaaaccatg
     1861 aggcagacac gtatcatcac cgcaaacata aagattatgg tatgttattg tgttgcccgg
     1921 tttacggcgc accatttaca actatactcg atcatgagtc atttttcaaa ccgtttaact
     1981 ggctcttagg agataattgt attgtttggg tttatacttc atcttgtatt gcaccaaagc
     2041 aaacaaattt tgcatcgaga attcatcttg aacgaactat tttcacgggc gattttattg
     2101 atggtgtagg cagtcttatt ttattagatg attttaatga aagtaacggt gcaacttatt
     2161 tgctaaaagg ttctcataaa aacttagaaa aaccggaaga tgagagattt tataaagaag
     2221 ctatccgctt ggttgctccg aaaggtacgg tgttgtattt taatttaaga ttgtggcatg
     2281 ccggcgggca aaatcataca gttaaatggc gcaattcgct gggcattggt gtcaatcgcc
     2341 cctacttgaa acaacgtatt gacattccca gaattttacc acaagcggtt aaagataaat
     2401 tatccgatta cgcaaaaaat aagttaggat ttaatgtaca agttccttca tcttttgagg
     2461 aatactaccg gaatgaaaaa aaatcatata caaacaaata gtttattaat ttatatacta
     2521 cttagcctca taatttcgtt tattcatttc atctgtttgc cataatgttt catcatattt
     2581 tgagagcatt ttcaagaagg aatagtgttc tttccacatt tgtccgaaac tgtcaaactc
     2641 gtaataaggg tattgatatt tatggcagag ttcttttaca attttcttta atttatgcaa
     2701 attctcaacc ggaataaaag gcaataaatg atgttcgatc tgaatgttaa aacctccgct
     2761 ttgccagaac caataattag aatctgcaca aaaattactg gtattatgac agacataata
     2821 acccgggtag tttttatacg cttccggaag tggtttaact gtttcgggaa taaaatgatt
     2881 gatggcggcg cttacataaa ctaaactgaa caatatacac tgatagcaca aataaaaaat
     2941 gaccaggaaa taccaactga tagtagtaca aaaagccgga agtaatatga aataaaataa
     3001 agaaaacaag atggataaat tatgcaacca ggctactttg cggttatgga tattccagaa
     3061 atcagaacta atgcctccat atgtagtaat tatatttgca taaaaagggt aaaaaaacat
     3121 agctaattta tgctgatatc tgtgcagaaa atttggtgta atattatttg aatatcgaat
     3181 gaattttgaa aaacctgcga cttcaaaatc atatgctgta tctccaatct ttatatgatg
     3241 actgttgtgc tcataacgca gtgccttttc cgaaataaac ggccagcaca aaggccaata
     3301 aatgctcaat actattttgg aaagtcgagt tttaggataa ttgcgatgag ttgcttcatg
     3361 tgccaaaaaa ccaaacaaag atgcacctac cattgcctgc aatatgataa gcaggctttt
     3421 ataaattatg gctaattgcg tataaaaaac aagtgcataa catacaaaga aaacgagtaa
     3481 attaaagaat cttattagat taaaactgcg aagttctttt tttgaaaccg tatataaatt
     3541 ttgtttcatc actgactcta gcagctcatt caagaagggt tctttcaaag cttcaagatc
     3601 aatcttttca gatgcacata tttctcctat acaatatgtt tctaattgcc ttaatatacc
     3661 tttattcgaa acatgtttta gataaaaaaa acttgtagca tcctctcctg ctctagtttc
     3721 aattatctct tttcctccag gatgtttttt tataaaatca gataaatcat aaaccttatt
     3781 gttaataatc acccaacagt catctaaagt attgtgttgc aaaacctcac ttctattata
     3841 actcttttgc atagctttac tttacaaaaa tactaaaaac cgtttacatt aagcatgaaa
     3901 gcagggtaag ttattaagaa ctgtagttta taaaaacgct attgaaaagt tacttagcac
     3961 aacaagtatc aattcttata tttatataaa attaccgaac ctatcgtatc tgccggctga
     4021 tataaggaat aatatttctt aataaaaaaa tctctgtcaa ttggacttgc tatatacgga
     4081 acagatccat ccagaaactt cttgttatag gttgaaggat taaaaaaaac agggtaagaa
     4141 tacactcctt tcggtaataa tttataaact tttaagggca aatacacttc cataaaacga
     4201 gtgatattat agtcataccc gtatataaga ttgcaatgat gataaaaaaa aacattgcgc
     4261 aaatccgtta caaaaatggg tttgtcttgt atcatttcat gtatttgctg tacttctttt
     4321 actttttgca tgtagctggt ttttaaaatc tcttgttctt tgaaactata attatagctg
     4381 tgtattatat aatttgttgc aaataaatac aataaaaaaa aacatgtgaa taatttatag
     4441 tttatcagat acaatttttt tctttcattg ctatataaaa ccaacaatgc aaaaaccagt
     4501 agaaaaacag tttcgtatcc atagtttaaa taagagccgg ctcgtaacat aaaaaaatgt
     4561 gaaacaacta aaaagacagc tgtccatatt ttgagcaaaa cgcttatact tcttttttca
     4621 gttttataga aaaataaaaa taagaatatt ggcatcaggc gtgataaact caatagtact
     4681 acaaataaat taaatgaaga gtaaactttt gtcagctttt gaaaattaaa taataccaca
     4741 tgatcaaaaa aataagcacc gaaatacaat ctataacatg acaaccaaac aaccagtaac
     4801 agcatgtagc acatccatga aagaaagtat ttataatctt tccgaaatag aaatgttgct
     4861 aggagtatca gtgaactata aataagcatg tcttgtttaa caacagtact tagcagaaag
     4921 aaaaaaacag cgctgagtaa tatccaattc tttttagtaa agaaagtata ttcaactaaa
     4981 aaaagtattg cagtgttaaa aaacaatact ttaagggcat ctggtcgcat aacgtacatg
     5041 tgcccggaaa tcagcaacaa gtacaacatg acaccaaaaa gtaaattcct gtttgaaatt
     5101 aagaatctct ttttacaata aaaaaaagac aggcatactt gtaatagaaa aaccgaaaaa
     5161 gaaaaagctc tgccaataat aaaaataaca tgcggatctg tcttagaacc tgcaccaaaa
     5221 atctcaacca aaaattttaa tacataaata tatgttggca aaaaaagaca tacaaagtat
     5281 ggataatccg tagggtttcc atataatggc tgattgtccg tcagacgttg cacatatatc
     5341 acaaacgccg attccatacc atctaaatct actgagtatg aaaatataag taatagtcga
     5401 actgtcaaaa gaaacagtat taccataaca atcaagactg tttcccatct tttttcgcca
     5461 atttcctgtt gcatttcaaa atgatgtaat ataatcataa aattgttatt tatgctctta
     5521 tttttttgta gcttagaact caattcaaat taaatgaaat cactttattt taccgaagaa
     5581 cacgaattat tcagggacag tgttcgcaaa tttgtggaga aagaaatagt gccaaatgcc
     5641 aatcaatggg aaaaagacaa aaaaatacca cgcgatttat ttttaaaatt aggcgaacaa
     5701 gggtttctcg gcatcaacca cagtgaaaaa ttcggcggct ctgatgccga tattttctat
     5761 acctgcgcct atcttgaaga gatggcaaaa agcacgtatg cgggcgtttg tgctgctgtt
     5821 tcggtgcatc aatacatggc taccaatcat ttggcagaag ccggcagcaa agaacttaaa
     5881 gagcgatttc tgcgtccttc tatcgaaggc aaaatggttg gtgctatcgc tattaccgaa
     5941 cctttcggcg gatctgacgt gcaagccatg cgcactactg ccattaaaga tggcgactac
     6001 tatatcatca atggctctaa aacatttatc accaacgggc atttttgcga ttttgtggtg
     6061 gtggcttgca aaactgataa caatgccggc atcaatggca ttagtttaat tgttgtagaa
     6121 cgaggcacac cgggttttac gtccacacaa ctcgaaaaaa taggctggca ttcttctgat
     6181 accggagaac tggctttcga taatgtcaaa gtgccggtta acaacctcgt aggtaaggaa
     6241 ggaatgggct ttttttatat catggaaagc tttcaggtgg aacgactcgt agccggaatt
     6301 ttaggcatcg gtggtggcga agtttgtttg gatgccactt tgaattacat gaatgaacgg
     6361 gaagcatttg gtcgtcagat taagaaattc caggtgctgc gccatgatat ggtacaactc
     6421 tacacggaat tagaggccgg gaaacaaatg acttaccacg cttgttggtt attgcaaaat
     6481 ggtgaagttc ccgtgaaaga atgtacgatg gtaaaattgt atatgacgga gctcggcaac
     6541 aaaatcgttg acaaatgttt gcagatgttc ggtggctatg gctacatgga agatttcgcg
     6601 atagcaaggg cttaccgcga tgcacgagtg ggcactatcg taggtggcac cacacaaatt
     6661 atgcgcgaaa ttttatcgaa gattattatt gatgatgtgc gctacaaaaa ggtatataat
     6721 gaaaccaatg aattaacaaa tacaccgact gaaaacaaag aaaaatcttg ggggcatccg
     6781 ctgacggcta aagaaatcat acattccatt ccattgcgca tcaaacatga taaagcggca
     6841 gcatattcta ctgtttttca ttttgatatc agtggtgaaa acggcggaca attcactgta
     6901 aaaatagaaa acggaaatgc tgtggtaagt gatgattttg aaggcacacc tgattgtgtg
     6961 gttacagcaa gcgacacggt ttatgccgat attgaattgg ggaaactaga ccctaccatg
     7021 gcatttatgg gtggacaaat aaaagtgagc aatatcggag cgatgatgca gttttcaaaa
     7081 tattttcacc gaatcacctc gtaggttgaa ttttgccacc tcgtaggtta aaatacaaat
     7141 ccttgatttt cagtagtttt ttaaatgaaa atcaatatat cgacctcgta ggttggcttc
     7201 gccaacctac gaggttgtca atttctaata attcactacc tttgtaaata tgtcgagcga
     7261 aattttgcta caacaccggc aaattcaatt aattattgag cgattaagcc gtcagataat
     7321 cgaatcgctt gatttaagga ataccgttat catcggcgtg caaccacggg gaacactttt
     7381 cgctgaaaaa atccatcata atattgaaca attaagcaaa ctcaaaattc cattcgggaa
     7441 aatagacact acctttcacc gtgatgattt cagacgaggt aaaaacctca acgccaaagt
     7501 aaccgagatt gattttttgt tagaaggaaa gactgtctta ctattggacg atgtgcttta
     7561 taccggcaga acgatacgtg ctgcaatgga tgcgatgcta acatttggca gaccggaaaa
     7621 agtagaactc atggtgctga tagaccgcag atttaaccgt gaattgccca tacaggcaga
     7681 ttttatcggc aaaacggtgg acactatcga gcacatcaac gtagaagtaa tatgggacag
     7741 tgctaatgca gataataaag tgatatttat aaacgaataa aaccatgagt cagttaagct
     7801 caaaacattt aatcggcata gaacatctta ccaaagaaga tttggagttg atattgacca
     7861 ctgccgttga gtttaaagac atcatcaacc gaccaataaa aaaggtaccc actttacgcg
     7921 atatcactat agctaattta ttttttgaaa attcaacgcg cacacgcatc tcgttcgagt
     7981 tggcacaaaa acgcctgagt
//
LOCUS       DAOQII010000099         7942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4947_length_7942_cov_4.114746, whole genome shotgun sequence.
ACCESSION   DAOQII010000099 DAOQII010000000
VERSION     DAOQII010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4947_length_7942_cov_4.114746"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLF48_06140"
     CDS             complement(<1..168)
                     /locus_tag="PLF48_06140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNF48941.1"
                     /translation="MTIFSKIIAGEIPSYKIAEDKQFYAFLDIRPLNYGHTLVVPKRE
                     VDYIFDMDDKEL"
     gene            339..923
                     /locus_tag="PLF48_06145"
     CDS             339..923
                     /locus_tag="PLF48_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48942.1"
                     /translation="MHTIEPHWKWRDFYSAEEDELSPFYGKEYSEFEFSDTIYNYYIH
                     PQWDFFGSNTLYLKILYCDYKKGSAIIEFIGEWNDCVNNDILFLKRDIIDDMIANGIT
                     KFVLIGENVLEFFANSNDYYDEWYADIENEGGWIIAVNFRTHVMEEMRMAKIDRFVHI
                     SERYADINWRSYKPFHLVTYLDELLIKTISYKNE"
     gene            916..1284
                     /locus_tag="PLF48_06150"
     CDS             916..1284
                     /locus_tag="PLF48_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF48943.1"
                     /translation="MSNRVVHFEIPSEHPERSISFFKEVFDWEITPFREGEYWLIKTG
                     ADDEAGINGGIIKKKDPRQPLATTIQVENLNVTVKKIEAAGGKTVTPKMIIPTVGYLI
                     YFQDLDGNIHGLMQRDTTAE"
     gene            1309..1983
                     /gene="rsmI"
                     /locus_tag="PLF48_06155"
     CDS             1309..1983
                     /gene="rsmI"
                     /locus_tag="PLF48_06155"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNF48944.1"
                     /translation="MSKLYIVPTPIGNLQDMTLRAINILQSVDLILAEDTRNTKFLCQ
                     HFAIETPVQSYHKDNEHKVLSGLIAKLQTEMQMALVSDAGTPGISDPGFLLVRECIKN
                     NIAVECLPGATAFVPALINSGFPCDKFVFEGFLPHQKGRQTRWLALKEETRSIVFYES
                     PYRVIKFLNEAKEFCGEDREMAVSRELTKKFEETVRGSILTVLNHFTNKEPKGEFVIV
                     LKGKSS"
     gene            1980..2774
                     /locus_tag="PLF48_06160"
     CDS             1980..2774
                     /locus_tag="PLF48_06160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNF48945.1"
                     /translation="MKTLPQILADALPVVKEAASFIQSEIQKSHRGVVEIKSLNSLVT
                     YVDKNAELILVGGLKMILPEAGFLTEENTIEQTKNDWQWIIDPLDGTTNFIYKIPVFA
                     ISVALAYRDEIQLGIVYELNRDECFTAIKNEGAFLNGQLISVSSTQHLKDSLVATGFP
                     YYDYAMLENYISLFKELMTDTRGIRRLGSAAIDLCYTACGKFDAFFEYSLSPWDVAAG
                     ALILQEAGGKITDFEGGTNWLYGKQILGCNQQIFEEIFDKIKRNFK"
     gene            complement(2794..3540)
                     /locus_tag="PLF48_06165"
     CDS             complement(2794..3540)
                     /locus_tag="PLF48_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HNF48946.1"
                     /translation="MSIQLADISTVAPKKLEKNEIKKETKKILEEIADLQDMLIASKK
                     RSLLVILQGMDASGKDGAVKNIFGGLNPLGIKVQTFKKPTEEEFAHDFLWRIHKVVPQ
                     KGDITIFNRSQYEDVLIQRVHKWIDEETVKQRFDAINNFEQLLENNGTTIIKCYMHIS
                     LEAQLERLEERKTNPKKMWKHNAQDWEERKLWKEYMQAYEDVFTNCSKAAKWNIVAVD
                     QNWYKEYQIAVLVRDALVAMKLSYPELKEE"
     gene            3731..4897
                     /locus_tag="PLF48_06170"
     CDS             3731..4897
                     /locus_tag="PLF48_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF48947.1"
                     /translation="MIIPAAEKLTHINEYYFSTKLKQIAQMRAEGKDIINLGIGNPDL
                     PPSKETLQALSASASNIKNHGYQSYVGIPALRKAMSNWYKSTYEVELHPETEILPLLG
                     SKEGISFISQAFLNPGDKVLVPNPGYPTYTSATYLAGAIPVYYDLDENNNWLPDFHAI
                     PEEVWKDIKLIWINYPNMPTGSKGNPADFIKLIKIAKEHRVLICNDNPYSLVLNDEHP
                     VSFLQTPEAMEVCVELNSLSKSHNLAGARIGMVCGRADYLQTILKVKSNVDSGMYLPV
                     QEAAVVALQNSATWHEERNTVYKKRREVAYHILDLLQCQYTKEQVGLFLWAKIPDSYH
                     QVEDLTEKILHEANVFITPGFIFGSNGARFIRISLCSNEMELTQAYERIKRIFA"
     gene            complement(5067..6347)
                     /locus_tag="PLF48_06175"
     CDS             complement(5067..6347)
                     /locus_tag="PLF48_06175"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNF48948.1"
                     /translation="MITINVFWFRRDLRLEDNNALQAALQAGLPVLPIFIFDVNILND
                     LAKNDARVQFIHQQLVEIDKTLKKAGSSLLCKKGEPLHIWKLLLSEYHINAVYTNEDY
                     EPYAIRRDKLIKALLQENNISFQLFKDQVVFEKDEIVKDDGKPYTIYTPYKNKWLAKF
                     SSQNLQDAQKPDVTKFYQFYANFPSLSEIGFFPNAIQVKPINPDALLHYQDKRDYPSM
                     ETSNTSVHLRFGTISIRQLVKSLQETDAVYLSELIWREFFMQILFHFPYVVSGAFRKE
                     YDHILWLNQESDYEAWCKGKTGYPLVDAGMNELNATGYMHNRVRMVTASFLCKHLLID
                     WRWGEAYFASKLLDYDLSANNGNWQWAAGTGCDAAPYFRIFNPGSQQEKFDKNFEYIK
                     KWNPDFQKTKPIIDHDFARKRALATYKAGLAIKQ"
     gene            complement(6360..7559)
                     /locus_tag="PLF48_06180"
     CDS             complement(6360..7559)
                     /locus_tag="PLF48_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF48949.1"
                     /translation="MPSLSEKGKMMPASPIRKLAPFADQAKKSGIHIYHLNIGQPDIE
                     TPYQFWDALKKIDRTVLEYSPSNGFEETRNKYAAFFKKRYGTEITGEEVMITTGGSEA
                     LLFTLFSILDMDDEIIIPEPLYANYIGFAKSGNIKVVPIPTDFENHFALPSVASFEAK
                     ITAKTKAILICNPNNPTGYFYSKNELEQLEILAIKYNLFLICDEVYRDFIYDKDLEYT
                     SILSFPNLKDHAILIDSLSKRFSVCGARMGMIATRNKEVLATALKFGQQRLSPPTVEQ
                     LGAMALFDVEENYFEKVNKEYKKRRDTLVEHLNKIDGVKCHTPGGAFYCIVQLPVKDA
                     DDFCRWMLSDFHYNQETVMMAPASGFYATEGKGKNEVRIAYVLQSPEIIKAVKCLEEG
                     LKKYLSK"
     gene            complement(7631..>7942)
                     /locus_tag="PLF48_06185"
     CDS             complement(7631..>7942)
                     /locus_tag="PLF48_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF48950.1"
                     /translation="GSQAQGKPVTYEFEFTNVGKEPLIIENVKASCGCTTPGWTKEPV
                     MPGKKGMVSAQYNMAREGNFTKTITVTTKDGETITLYIKGNAVPMKQGVDEAEGSIIG
                     G"
BASE COUNT         2447 a         1410 c         1511 g         2574 t
ORIGIN      
        1 caattcctta tcgtccatat caaaaatata atccacttcg cgtttaggta ctactaaagt
       61 atgaccgtag ttgagtggtc ggatgtccag aaaggcataa aattgtttgt cttccgcaat
      121 tttatatgaa ggaatttcgc ctgcaattat tttactgaat atggtcataa attttatttt
      181 gtagtctgta actttaatgg gattaatctg tctgatgctg tgcaaaatta agagtattta
      241 ttgtcaactg atagtttcaa ggctttttta tctaaataag tattaaaacc attgttcatt
      301 ttttattttt agtttatctt gctctagtat attgattaat gcatacgatt gaaccacatt
      361 ggaaatggcg cgatttttat tcggcagaag aggatgagtt atcacctttt tatggcaaag
      421 aatacagcga gttcgagttc tcagacacca tatataatta ttatatccat ccgcaatggg
      481 attttttcgg cagcaataca ctttatctga aaattttgta ttgcgactat aaaaaaggtt
      541 cagcaattat tgaatttata ggcgagtgga atgattgtgt caataatgat atcttgtttt
      601 taaaaagaga tattattgat gatatgattg ctaatggtat tacaaaattc gttctgatag
      661 gcgaaaatgt actggagttt tttgcgaaca gcaatgatta ctatgacgaa tggtatgcag
      721 atattgaaaa tgagggcgga tggataatag ccgtaaattt caggacgcac gtgatggaag
      781 aaatgcgaat ggcaaaaata gatcgttttg tgcatatcag cgaacgctat gcggatataa
      841 actggagaag ttataaaccc tttcacttag taacttacct cgatgaatta ttaattaaaa
      901 caatatctta taaaaatgag taaccgagtt gtacatttcg aaattccatc agagcatccg
      961 gaaagaagca ttagtttttt taaagaagtt tttgattggg aaatcacccc attcagagaa
     1021 ggcgaatact ggctgataaa aacaggagct gacgacgaag ccggaattaa cggtggaatt
     1081 ataaaaaaga aagacccaag acaaccgctg gccaccacaa ttcaagtgga aaacctgaat
     1141 gtaacggtaa agaaaattga agccgcagga ggaaaaaccg ttacacctaa aatgatcatt
     1201 ccgacagtag gttatttaat ttattttcag gatttggatg gcaatattca cggcttgatg
     1261 cagcgagata ctactgccga ataatttttt gacttttaaa agttcatttt gtctaagtta
     1321 tacatagttc ctacgcccat tggcaatttg caggatatga cgctcagggc gattaacatt
     1381 ctgcaaagtg tagatttgat tttagcggaa gatacacgca ataccaaatt tttatgtcag
     1441 cattttgcta tagaaactcc tgtacaatcc taccataaag acaatgaaca taaagtttta
     1501 tcgggtttga tagccaaatt gcaaacagaa atgcaaatgg ctttagtttc tgatgcgggt
     1561 acgcccggta tttccgatcc cggcttttta ttagtgcgcg aatgcataaa aaacaacatt
     1621 gcagtagaat gtttgccggg ggcaactgcc tttgttccgg ccttgataaa ttcagggttt
     1681 ccttgcgata aatttgtttt cgaaggattt ttaccccatc aaaaaggcag acaaacaaga
     1741 tggctggcac tgaaagaaga aaccagaagc atcgtatttt atgaatctcc gtacagggta
     1801 ataaaatttt taaatgaagc taaagaattt tgcggggaag accgtgagat ggccgtaagc
     1861 agagagttaa ctaaaaaatt tgaagaaaca gtaagaggca gcatactgac ggtactcaat
     1921 cattttacca ataaagaacc caaaggcgaa tttgtcattg tattaaaagg aaaatcgtca
     1981 tgaaaacatt gccacaaatt ttagcagatg ctttgcctgt agtaaaagaa gcagccagtt
     2041 ttattcaatc agaaatacaa aaatctcatc gcggtgttgt tgaaataaaa agcctgaata
     2101 gtttggttac ttatgttgat aaaaatgctg aattgatttt agtgggtggt ttaaaaatga
     2161 tactgcctga agccggattt ttaaccgaag aaaatacaat agaacaaact aaaaacgatt
     2221 ggcaatggat aatagatccc ttagacggaa cgaccaattt tatctataaa attccggttt
     2281 ttgctatctc tgtggcattg gcttaccggg atgaaattca attaggcatc gtgtatgagc
     2341 tgaatcgtga tgagtgtttt acggccataa aaaatgaagg ggcattttta aacggtcaat
     2401 tgataagtgt ttcttcaact caacatttaa aagacagttt agtggctacc ggattccctt
     2461 actatgatta tgctatgctc gaaaattata tcagtttatt taaagaattg atgacagata
     2521 ctcgcggcat caggcgattg ggtagtgcag ctattgactt gtgctataca gcttgcggca
     2581 aatttgatgc tttttttgag tattctttat ccccctggga tgtggcagca ggtgcattga
     2641 ttttgcaaga agccggaggc aaaattacag actttgaagg tggtacaaac tggctttatg
     2701 gcaagcagat attgggctgc aatcaacaga tttttgaaga aatttttgat aagataaagc
     2761 gaaattttaa gtaagtgtat tttataaaaa atactattct tctttaagtt ccggataact
     2821 tagtttcata gcaactaagg catcacgcac caatacggca atctgatatt ctttgtacca
     2881 gttttggtct acggcaacga tattccattt tgctgctttg gaacagttcg taaatacatc
     2941 ttcataagcc tgcatgtact ccttccaaag ttttctttct tcccagtctt gtgcattgtg
     3001 tttccacatt tttttaggat tggttttacg ttcttcgagt cgttctaatt gggcttctaa
     3061 cgagatatgc atgtagcatt tgatgatagt agtaccatta ttttcaagaa gttgttcaaa
     3121 attattaatc gcatcaaatc gttgttttac cgtttcctca tctatccatt tatgcacacg
     3181 ttgtatcagt acatcttcat attgagaacg gttaaatata gtaatatcac ctttttgggg
     3241 aacaacttta tgaatacgcc ataaaaaatc gtgggcaaat tcttcttcag tcggtttttt
     3301 aaatgtctgc acttttatac ccaacggatt taatccgcca aaaatgtttt taacagcgcc
     3361 gtcttttccg ctggcatcca taccttgtaa aataaccagc aaacttcgct tctttgaagc
     3421 aataagcata tcttgcaaat ctgctatttc ttccagtatt ttttttgttt cttttttaat
     3481 ttcgtttttt tccagttttt ttggagctac cgtagaaata tcagccagtt gaatcgacat
     3541 agtattattt ttaaatgatt aaaggttgaa gatacaaaaa tagccaacat aaatctccta
     3601 atttagttca tgtgaaaata tgtttcagct tgtttgatta atgaaaacct aatcttagta
     3661 cataagataa ctatgaagtt tatagtagtt ttgtaaaaaa caatttttct ttcaacttta
     3721 attctggttt atgattattc cggcagcaga aaaacttacg catattaatg aatattattt
     3781 cagtaccaaa ctgaaacaaa ttgcacaaat gcgtgcggaa ggaaaagata ttattaacct
     3841 aggcattggc aatcctgatt tgcctccatc aaaagaaacc ttacaagctt tatccgcttc
     3901 agcaagtaac ataaaaaacc acggttatca aagttatgtt ggaattccgg cactgcgtaa
     3961 agcaatgagc aattggtata aatcaaccta cgaggtggaa ctccatcccg aaactgaaat
     4021 attgccgctc ttaggttcta aggaaggtat ttcgtttata tcacaggcat ttttaaatcc
     4081 gggggataaa gtgctggtgc ccaatcccgg atatccaacc tatacttcgg ctacttatct
     4141 ggctggtgct attcctgttt attatgattt ggatgaaaat aataactggc tgcctgattt
     4201 tcatgccata ccggaggaag tatggaaaga tattaaactt atctggatta attatcccaa
     4261 tatgcccacg ggttcaaaag gcaaccctgc cgattttata aaactcataa agattgccaa
     4321 agagcacagg gttttgattt gtaatgataa tccatacagt ttagtattga atgatgaaca
     4381 tccggtttcg tttttgcaaa ctccggaagc catggaggta tgtgttgaat tgaattcctt
     4441 gagcaaatca cataatttgg caggagcccg tatcgggatg gtgtgtggtc gtgccgatta
     4501 tctgcaaaca attttgaagg taaaaagtaa tgttgacagc ggtatgtatc ttccggtgca
     4561 ggaagcagca gtggtagcac ttcaaaattc tgcaacatgg catgaagaac gtaatacagt
     4621 ctataagaag aggagagaag ttgcctatca tattttagat ctgttgcaat gccaatatac
     4681 aaaagaacaa gtgggtttgt ttttatgggc aaaaattcct gacagctatc accaagtaga
     4741 agatttaacc gaaaaaatat tacatgaagc aaatgtattt attacaccgg gctttatttt
     4801 cggttctaat ggcgcacgct ttatccggat atcattgtgt agtaatgaaa tggaattgac
     4861 tcaagcttat gaacgcatta aacgcatctt tgcataaata ttgtggatta agttagtcaa
     4921 ctgtaaaaat acttgattta gccttacttc ttccaccttc atcaaggagg aggaaaaaaa
     4981 ggaggtggtt ctaaaaaaaa actaaatcaa ctattttagg atattcaaca ttaaatcata
     5041 cttaacttaa cgacattgaa tgtggattat tgttttatag ctaatcctgc cttataggtg
     5101 gccaatgctc ttttgcgggc aaaatcatga tcaataatag gttttgtttt ctgaaaatca
     5161 ggattccatt tttttatgta ttcaaaattt ttgtcaaatt tttcctgttg gctgccggga
     5221 ttaaatatgc ggaaataagg tgcggcatca cagccggtac ctgctgccca ttgccagttg
     5281 ccattattcg ccgacaaatc atagtctaat aatttgcttg caaaataggc ttcgccccaa
     5341 cgccagtcga taagtaaatg cttgcataaa aaactggcgg ttaccatgcg tacacggtta
     5401 tgcatgtatc cggtggcatt cagttcgttc attcctgcat ctaccagcgg atatcctgtt
     5461 ttgcccttac accatgcttc ataatcactc tcctgattga gccataagat atggtcatat
     5521 tcttttctga atgcaccgga tacgacatag ggaaaatgaa ataaaatttg cataaaaaac
     5581 tcacgccaaa tcaactcgga taggtaaact gcatctgttt cttgaagtga tttgaccagc
     5641 tgccgtatgc tgattgtgcc gaatcgcaaa tgcacactgg tgttgctggt ttccatactc
     5701 ggataatctc ttttatcctg atagtgtagc aaggcatcgg ggtttattgg ctttacttgt
     5761 attgcattgg gaaaaaatcc gatttcagat aatgaaggaa aatttgcata aaactgataa
     5821 aatttggtga catccggctt ttgagcatcc tgcaaatttt gtgatgaaaa tttggcgagc
     5881 catttatttt tataaggtgt gtaaatagta tagggcttgc cgtcatcttt cacaatttca
     5941 tctttttcaa aaacaacctg atctttaaac agttgaaagc tgatattgtt ttcttgcagc
     6001 agtgctttta ttagtttatc tcttcttatt gcatacggct cataatcttc attagtgtaa
     6061 actgcattta tgtgatattc gctcaagagt aatttccaga tatgcaaagg ttcacctttt
     6121 ttgcataaca aactactgcc tgcctttttc agcgttttgt ctatttcaac aagttgctga
     6181 tggataaact gtaccctggc atcattctta gctaaatcat tcagaatatt aacatcaaaa
     6241 atgaaaatag gcagaacagg tagaccggct tgtagggcag cttgcaaagc gttgttgtct
     6301 tccagcctta agtcgcgacg aaaccaaaat acattgatag ttatcatatc ttgctagtgc
     6361 tatttggaaa gatatttttt taagccttct tccaagcatt taacagcttt gattatttca
     6421 ggagattgta atacataagc aatgcgtact tcatttttac ccttgccctc ggtggcataa
     6481 aaaccgctag caggcgccat catcactgtt tcttgattgt aatgaaaatc actcaacatc
     6541 caacggcaaa aatcatcagc atctttgaca ggtaattgaa caatacaata aaaagcacca
     6601 cccggggtat gacattttac accatcaatt ttattgagat gttctaccaa tgtatctctg
     6661 cgctttttgt attctttgtt tactttttca aaataatttt cttccacatc aaacaaagcc
     6721 attgcaccca gttgttctac ggtaggcgga ctcaatcttt gctgaccaaa ttttaacgct
     6781 gttgccagca cttctttatt tctggtggct atcatgccca ttcttgctcc gcaaacacta
     6841 aatcgttttg ataaggagtc aattaaaatg gcatgatctt tcaggttggg gaaactgagg
     6901 atagaggtat attccaaatc tttatcataa ataaaatcgc gatatacttc atcgcaaatc
     6961 aggaaaagat tgtattttat agccagaatt tccagctgtt caagttcatt tttggaataa
     7021 aaataaccgg taggattatt gggattacaa attaaaatgg cttttgtctt agccgtaatt
     7081 ttagcctcaa atgatgcaac agaaggtaat gcgaaatgat tttcaaaatc ggtaggaatg
     7141 ggaacaactt taatatttcc acttttggca aagccgatat aatttgcata caatggctcc
     7201 ggaatgatga tttcatcatc catatccagt atggaaaata aagtaaacaa gagtgcctca
     7261 gaacctccgg tggtaatcat tacctcctct ccggtgattt cagtgccgta tcgttttttg
     7321 aaaaatgcgg catatttatt tcgggtttct tcaaagccgt tagacggact atattccaag
     7381 acagttcggt ctattttttt cagggcatcc cagaattgat aaggtgtttc aatatccggc
     7441 tggccgatat tcaaatggta aatgtgaatt cctgattttt tagcttgatc agcaaatgga
     7501 gctaatttac gaatgggtga tgccggcatc attttgcctt tttcggatag ggatggcatt
     7561 cgccaaaatt acaaaaaaat cccccgaaaa ctcgagggat gattatttat tttaatgatt
     7621 gaaatttata ttaaccaccg attatacttc cttcggcttc atcaactccc tgtttcatag
     7681 gtacggcatt gcctttgata taaagtgtta tggtttcccc gtctttggtc gtaacggtta
     7741 tggtttttgt gaaattacct tctcttgcca tattatattg agctgatacc atcccttttt
     7801 tgcccggcat aacaggttct ttcgtccagc ccggcgttgt acagccacaa gatgctttta
     7861 cgttctcgat aattaatggt tctttgccga cattggtaaa ctcaaattca taagttacag
     7921 gtttgccttg agcctgactg cc
//
LOCUS       DAOQII010000100         7930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_4960_length_7930_cov_4.376762, whole genome shotgun sequence.
ACCESSION   DAOQII010000100 DAOQII010000000
VERSION     DAOQII010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4960_length_7930_cov_4.376762"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..768
                     /locus_tag="PLF48_06190"
     CDS             238..768
                     /locus_tag="PLF48_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48951.1"
                     /translation="MKKIIFLLSLFVSVAMVQAKDKGPKVDMNGDTVTVDGVNQFIFE
                     KIKPVPGIYNYYIKSLDGKKLAFLKFQDYHDPKEISSANPNGRVTYYEVTFLESNQIA
                     EIMVYARQIKMAEYLVKLNLVKNGEITPEAEQEFLMENGREYSRKRNELNGGNTIIIN
                     NSEPEPKNKFNINIGR"
     gene            complement(886..1185)
                     /locus_tag="PLF48_06195"
     CDS             complement(886..1185)
                     /locus_tag="PLF48_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48952.1"
                     /translation="MINHENELPEFYKLFKYQLWTSILPAKSLIYLDKLNAFLFGVSK
                     FEDKKLLDIIIHDTHPVFFKWAIFQLLNWKATYTLKKIFIYMEMQTEYSLYQIQE"
     gene            complement(1186..2490)
                     /locus_tag="PLF48_06200"
     CDS             complement(1186..2490)
                     /locus_tag="PLF48_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48953.1"
                     /translation="MLKNESMKTTYSILVCCLLLFSCNAHSQDTVQRGKLYNARTLEV
                     NPKLSYALYIPQKENYSKRIAYFFFDPSGDGSYPLKIYQSIADSLGVVLIGNNASKNG
                     MSFNAIAENFAAISTEVLRKLEINKDNMCLWGFSGGAKAAMYCADNFDYHYAIYGGAV
                     YPEMQHQLDLLGINGKKDMNYTELLAYDISQQNNTNHLQIEWEGKHSWPDSLTATDAF
                     KWFLFRKMQKNEIKWDAKLIQKTNIAFVKNVNQLYKTKQYYKTYINGKKTIIMLDKLS
                     NVQPVKNIIGTVVKSQSFLNEMKQLENQYQKETKLKNQYIANLQTQDSLYWKTETNKL
                     YALANTDKYGIYERLLGFLSLAAYSVSTESFRQNKLDNLPNILALYRYADPQNAEYAF
                     LSAKYYLLKNDIIQAKAYKQVALNLGMDTSRFTQDELLSKLP"
     gene            complement(2541..2765)
                     /locus_tag="PLF48_06205"
     CDS             complement(2541..2765)
                     /locus_tag="PLF48_06205"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNF48954.1"
                     /translation="MYDDLVLIQTYFQVIDAQLAKSKLDAYDVFCFLKDENLVQNDWL
                     RTIAYGGVKLYVRAEDKARAEEILNDVQAD"
     gene            complement(2768..3223)
                     /locus_tag="PLF48_06210"
     CDS             complement(2768..3223)
                     /locus_tag="PLF48_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48955.1"
                     /translation="MRNLLLYISIGLACSCTVKTPTSSEVSATNSTIQDSILTNNQTE
                     IKSKADTEMPTVRVTGMEPFWNFNIKQDTFLFTFLTEKADSAYFTLDSFTQSENHIAY
                     YLKDVQAVPAVLKLMKTGKCSDGMSDKIYLYEASFYYKRMTLNGCAENQ"
     gene            complement(3225..4250)
                     /gene="ruvB"
                     /locus_tag="PLF48_06215"
     CDS             complement(3225..4250)
                     /gene="ruvB"
                     /locus_tag="PLF48_06215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNF48956.1"
                     /translation="MQNPNLDASGEYLSNTEKELEKVLRPQAFEQFSGQPQIIENLQI
                     FIQAAKKRDEALDHVLLHGPPGLGKTTLSHIIANELGVEMKMTSGPVLEKPGDLAGLL
                     TNLQPNDVLFIDEIHRLNTSIEEYLYSAMEDYKIDIMLDSGPSARSIQISLNPFTLIG
                     ATTRSGLLTAPLRSRFGITFRLEYYPATVLQHIIKRSSRILNTEIDEKGSFEIAKRSR
                     GTPRIANALLRRVRDFAQIKGNGKIDLEISQFALKALNVDEHGLDEMDNTILQAIIEK
                     FAGGPVGITTIATAVGEESGTLEEVYEPFLIQEGYLKRTPRGREATAKAYHHIGKVPP
                     SHTGTLF"
     gene            4338..4916
                     /locus_tag="PLF48_06220"
     CDS             4338..4916
                     /locus_tag="PLF48_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48957.1"
                     /translation="MKNTLLFFIAGSLCFISCKHKTEDSSNETTAATNEKNGTILFCT
                     GQAVETPHGDEINRYTDTRYDEEHVVQVSVEQDSFFMYETVKSKEKPDEYMLVDVSAF
                     AYKDMNPSTEEGVYTSEYDLDLTDGKKEHQTVFFIKAKNEERIFKQRTYTCIDPDGNN
                     FLQNDLFGANIACSSMEKAKEMVEKIRAKLPK"
     gene            4989..6455
                     /locus_tag="PLF48_06225"
     CDS             4989..6455
                     /locus_tag="PLF48_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNF48958.1"
                     /translation="MLKRVDKVVLGSFILPLVITFGLTTFIFMMQFLWKYIDDFVGKG
                     LETSLLFKLVALFALTIIPMALPLAILLASTMVIGNLGENNELTAYKSGGLSLLRIMG
                     SLIVFTFFLSITAFYFSNTILPKVHLKFFSLLYDIRKQKPALDIKEGVFYRGISGFAI
                     KIKKKAPDNKTLYNIMVYDHSNGKGNESVVMADTGKVELSNTGEFLVLTLFNGKQYQD
                     VKKRNNQSDESKAEQTTMSFKKWQKVFDLREFKMTRTDQNLFKSNYIMFNTRQLRQES
                     DSLQKDIQKKYVDIQTTDASYFSFLKDNNPKPDTNAGLNYAKMNFYKIFSRDITERAL
                     VSAKNNCNSVKSNMEMNASDLKYKEENYVNYICEWYRKFSLSFACMVLLFVGCAMGAI
                     VRKGGFGIPILIAVLYFVLFHVLNVVGEKLAKGLVIPPLFGMWLSTLVLLPLALFLTY
                     KANNDSVLFRSEWYDKYFYKIIAGIKNLFSRKKETQTT"
     gene            6452..7585
                     /locus_tag="PLF48_06230"
     CDS             6452..7585
                     /locus_tag="PLF48_06230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF48959.1"
                     /translation="MIVYLLIAFCLLVLLYAYVGYPRFLAKKATGKSLAYAAYLSQDE
                     LPCVYILIPVHNEEKVIIQKLKSVFESSYPQKKIQVLIGLDACTDNTKKNIQKNFSTY
                     PIQLFDFTERQGKPAMLNKLMAYATEKNAITIFTDADIFFNQNTLFELVKYFKDERIG
                     LVDTIIQTQRSTDNNEQFYWNYENEIKWNESLVYGKITGPSGGCYAIRTKLYEEIPVN
                     FVVDDFFIGMKIALKKYHTIVNKEALAIEEIQTNWIQEFRRKSRIASGNFQNLWYFKK
                     TAFQVYTPLGFIFFSHKFLRWKTPFILLILYYLLLFKATLLILIVTFILPVFDAICAI
                     FGWAIKPLRRLHYFLFMNLAVFWGFINFCKGIKSNVWQPTPRK"
     gene            7557..>7930
                     /locus_tag="PLF48_06235"
     CDS             7557..>7930
                     /locus_tag="PLF48_06235"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNF48960.1"
                     /translation="MSGNQHHANNIQLDTIESAVDAIKNGKLVIVVDDEDRENEGDFI
                     TAARNATPEIINFMATHGRGLICTPLIESRCDELKLELMVNANSSHYDTPFTVSVDLI
                     GEGCTTGISASDRAKTIQALINP"
BASE COUNT         2552 a         1321 c         1447 g         2610 t
ORIGIN      
        1 aatcagctgt ccgaaaacgg atgtaccgaa attatgcgta cttttattca tatagtagtt
       61 tgtgcaaaaa caaactatac aaattgagcc ataatcccaa aattatggct ctcttttatt
      121 atttttatcg gacactgtga tagtttatag caatgttttt taagttatca gttcaattcg
      181 aaaataaaat taatccgtag caattaatta tttatatttg ctccttaaaa taatagaatg
      241 aaaaaaatta tatttctgtt aagcttgttt gtaagtgttg ctatggtgca agccaaagac
      301 aaaggaccca aagtggatat gaatggtgat acggtaaccg tagatggtgt aaaccagttt
      361 atttttgaaa agattaaacc cgtaccgggt atctataatt actatataaa aagtttagat
      421 ggcaagaaat tggcattttt aaagtttcaa gattatcatg acccaaaaga aattagttca
      481 gcaaatccaa atggcagagt tacgtattat gaagtaacgt ttttagaaag caatcagatt
      541 gctgaaataa tggtttatgc ccgccaaata aaaatggctg aatacctcgt taaactaaac
      601 ttggtgaaaa acggagaaat aactccggag gctgagcaag aatttcttat ggaaaatggt
      661 cgtgaatatt ccagaaaaag aaatgaactc aatggtggca atacgattat catcaataat
      721 tcagaacctg aaccaaaaaa taaatttaat attaatatag gaagataatt ttttctttag
      781 tcaatttacc tgaattagca attacttatt tcgttattta ataactgcac aatttgttgt
      841 gcatggtgac aatcataaaa tgaccaccat tattgacaat atgagttact cctgtatttg
      901 gtagagggag tattctgtct gcatttccat gtatatgaat atctttttga gcgtataagt
      961 tgctttccag tttagcagtt gaaagattgc ccatttaaaa aaaacagggt gagtatcgtg
     1021 aataataata tccaataact ttttgtcttc aaatttactt acaccaaata agaaggcatt
     1081 cagcttatcc aaatagataa gagatttagc aggtaaaatg ctagtccaaa gttgatactt
     1141 aaagagcttg taaaactccg gaagttcatt ttcatgatta atcacctaag gcagcttact
     1201 caatagctca tcttgtgtaa aacgcgaagt gtccatgcct aaattgagtg cgacttgctt
     1261 ataggcttta gcttgtatta tatcgttttt cagtaaataa tattttgctg ataaaaatgc
     1321 atactccgca ttttgtgggt ctgcataacg atataaagcc aggatattgg gtagattatc
     1381 tagcttgttt tgcctgaaac tttcagtaga aactgaatag gccgccaatg acaaaaaacc
     1441 taataagcgt tcgtaaattc catatttatc ggtattagcc agtgcataga gcttattggt
     1501 ttctgttttc caataaagag aatcttgagt ttgcaagttt gcaatgtatt gattttttag
     1561 tttggtttct ttctgatact gattctccag ttgtttcatc tcatttaaaa aggactgact
     1621 tttcactact gtgcctataa tgttttttac aggctgcaca ttgctgagct tatctagcat
     1681 aataatcgtt tttttgccat tgatataggt tttatagtat tgttttgttt tataaagttg
     1741 gttgacattt ttgacaaatg caatattggt tttttgaatc aactttgcat cccatttgat
     1801 ttcatttttt tgcatcttgc ggaataaaaa ccatttaaaa gcatctgttg cggtgaggct
     1861 gtccggccag ctatgtttgc cctcccattc tatttgcaaa tgattggtat tgttttgttg
     1921 tgaaatatca taggcgagta attcggtgta attcatatct tttttcccat ttatacctaa
     1981 taaatccaat tgatgctgca tttcgggata aacagcaccg ccatagatgg catagtgata
     2041 gtcaaaatta tcagcacagt acatggccgc tttagcgcca ccggaaaatc cccataaaca
     2101 catattgtct ttattgattt ccagtttgcg gagtacttca gttgagattg ctgcaaaatt
     2161 ttctgctatg gcattaaagc tcattccatt tttggaggcg ttgttaccaa tcaagactac
     2221 acccagactg tctgcaattg attgatatat tttcaaggga taacttccat ctccggaagg
     2281 atcgaaaaaa aagtatgcga tacgtttact gtaattttct ttttgaggaa tatacaaagc
     2341 atacgacaac ttgggattaa cttctaaagt acgagcgtta tataacttgc cacgctgcac
     2401 ggtatcctga ctgtgggcat tacacgagaa gagcaacaag caacaaacta aaatggaata
     2461 agttgttttc atcgattcat tttttagcat caactaaatt acagaagttg ctgacaggat
     2521 actgctttag attaaaaaaa ctaatctgct tgcacatcat tcagaatttc ttcagctcta
     2581 gctttatctt cagctctcac atacaatttt acacctccgt aagcaatagt gcgcaaccag
     2641 tcattttgta ctaaattttc atctttcaaa aaacaaaata catcatatgc atccagtttg
     2701 gattttgcca gctgtgcgtc tatcacttga aaataagttt gtatcaacac taaatcatcg
     2761 tacatggtta ttgattttct gcacaaccat ttaaggtcat gcgtttataa taaaatgagg
     2821 cttcataaag ataaatttta tcactcatcc catccgagca ttttcctgtt ttcattagct
     2881 ttaatactgc aggcacagct tgtacatctt ttagatagta agcaatatga ttttctgact
     2941 gcgtaaaact atcgagcgta aaataggctg agtctgcttt ttctgtcaaa aaagtaaata
     3001 gaaacgtgtc ttgctttatg ttgaaattcc agaatggttc cataccagta accctaactg
     3061 tcggcatttc tgtatcggct ttagatttaa tttctgtctg gttatttgtc aaaatactat
     3121 cttgaattgt cgaatttgta gccgacactt cacttgaagt tggtgttttt acagtacagg
     3181 aacatgctaa cccaattgat atatataaca gtagatttct catgttaaaa taaagttccg
     3241 gtgtgagaag gcggtacttt cccaatatga tgatacgctt ttgccgtagc ttctctgccg
     3301 cgaggtgtac gtttcaggta gccttcctga attaaaaacg gttcatatac ttcttccaac
     3361 gtaccggatt cttcaccaac agcagtagca atagtagtaa taccaacagg acctcctgca
     3421 aatttttcaa tgatggcttg caaaatggta ttgtccattt catcgagtcc gtgttcatcc
     3481 acattcaatg ctttcaaggc aaattgcgaa atttccaaat ctattttgcc attgcctttt
     3541 atctgtgcaa aatcgcgtac acggcgtagt aaggcattag caatacgcgg agtacctctg
     3601 ctgcgcttgg caatttcaaa cgaacctttt tcgtcaattt cagtatttaa aatgcgtgat
     3661 gaacgtttga tgatatgctg caatactgtt gccggataat attccaagcg aaaggtaata
     3721 ccaaaacgag aacgcaaggg tgctgtaagc aaaccggaac gtgtggtagc gccaatgagt
     3781 gtaaacgggt tcagtgaaat ttgtatgctg cgcgcactgg ggccgctgtc gagcatgatg
     3841 tcgattttgt aatcttccat agcggaatac aaatattctt caatactcgt atttaagcga
     3901 tgtatttcat caataaacaa aacatcatta ggttgcaggt ttgtaagcaa tccggcaagg
     3961 tcgccaggct tttccaatac agggccggaa gtcattttca tttccacccc caactcattg
     4021 gctatgatat gagataaagt tgttttgccc agtcccggag gtccgtgcaa caaaacatgg
     4081 tcgagtgctt catctctttt ttttgcagct tgaatgaaaa tttgcagatt ctcaataatt
     4141 tgcggctgac cggagaattg ttcaaaagcc tgcggtcgca gtactttttc taattctttt
     4201 tcggtattgg ataaatactc cccactggca tctaaattcg gattctgcat atggcaaaga
     4261 taaggatttc cttttttcgg ttaaaaaatt aaatatcaaa gaaatttttt acttttattt
     4321 taactaaaat acgttatatg aaaaacacac tcttattttt tatcgcagga agtttatgtt
     4381 ttatcagttg taaacataaa acggaagaca gcagtaatga aacaacagca gcaacaaatg
     4441 aaaaaaacgg aaccatttta ttctgtacgg gacaagctgt agaaactcca catggtgatg
     4501 aaataaatcg ttatacggat acacgttatg atgaagaaca tgtggtacaa gtgtcagttg
     4561 agcaggacag tttctttatg tatgaaacag ttaaatctaa agagaaacct gatgagtata
     4621 tgttagtgga tgtgtctgca tttgcatata aagatatgaa tccatcaaca gaagagggtg
     4681 tttatacttc agaatacgat ttggatttga ctgatgggaa aaaagagcat cagaccgttt
     4741 tttttatcaa ggctaaaaat gaagaacgta tatttaagca aagaacctat acctgtattg
     4801 acccggatgg taataatttt ttacaaaatg atttatttgg tgcaaatatt gcctgttctt
     4861 ctatggagaa agcaaaagaa atggtagaaa aaataagagc aaaactgcct aaataaaagc
     4921 agcagttaca aaattaattt taaaacattt atctgctaaa tgcgtatttt tagcatctta
     4981 ttaaagatat gctaaaacgc gtcgataaag tagtattagg ctcatttatt ttgccattag
     5041 tcatcacttt cgggctcact actttcattt ttatgatgca gtttttgtgg aagtatattg
     5101 atgattttgt aggtaagggt ctggaaacca gtttgttatt taagttagtc gcactatttg
     5161 ctttaaccat catcccgatg gctctgccct tagctatttt gctggcatct actatggtga
     5221 ttggcaattt aggtgaaaat aatgaactta cagcttacaa atcaggtggc ttatctctct
     5281 tgagaataat gggcagtctg attgttttta ccttcttttt atcaataacc gcattttatt
     5341 tttcaaatac tattttgcca aaggtacatc tcaagttttt ttctttgctg tatgatatca
     5401 ggaaacaaaa acctgcactg gatattaagg aaggagtatt ttatcggggt atctcaggct
     5461 tcgccattaa aataaaaaag aaagcacccg ataataagac gctatataat attatggtgt
     5521 acgaccattc taatggtaag ggtaatgaaa gtgtggtaat ggctgatacg ggaaaagtgg
     5581 aacttagtaa tacgggtgaa tttttagtac tgacgctttt taatggaaag caatatcagg
     5641 atgtaaaaaa aagaaataat caatctgacg aatcaaaagc agagcagacc actatgtcat
     5701 tcaaaaaatg gcaaaaagta tttgacttac gtgagtttaa aatgactcgt accgaccaga
     5761 atttgtttaa gtcgaattat atcatgttta atacccgtca attacggcaa gaatccgatt
     5821 ccttacaaaa agacatccaa aagaaatatg tggatataca gacaacagat gcatcttact
     5881 tttctttcct gaaagacaac aatccaaaac cagacactaa tgccggtctg aattatgcaa
     5941 aaatgaattt ctataagatt ttcagtcgtg atataacaga aagggcttta gtaagtgcta
     6001 aaaataattg caacagtgtt aagagcaata tggaaatgaa tgcaagtgat ttgaaataca
     6061 aagaagagaa ttatgtaaac tatatctgtg aatggtatag gaaattcagt ctttcatttg
     6121 cttgcatggt cttgttgttt gtgggttgtg ctatgggtgc aattgtacgt aaaggtggtt
     6181 tcggtatacc gattttaatt gcagttttat attttgtctt gtttcatgta ctcaatgtag
     6241 taggcgagaa gctggccaaa ggtttggtca ttcctccttt attcggcatg tggttgtcta
     6301 cattggtctt gcttccgcta gccttatttt taacttataa agccaataac gattccgttt
     6361 tattcagaag cgaatggtac gacaaatatt tttataaaat tattgccgga ataaaaaatc
     6421 ttttttcacg taaaaaagag acacaaacga catgatagtt tacctactga ttgcattttg
     6481 tctattagta ctcttatacg cttatgtagg ctatcctcgc tttttggcaa aaaaagcaac
     6541 aggtaaatct ctcgcttatg cagcttattt gtcacaagat gaattgccat gtgtctatat
     6601 acttattccg gttcataatg aagaaaaagt aattattcaa aaactgaaat ctgtatttga
     6661 atcaagctat cctcaaaaaa aaatacaagt gctgataggc ttggatgctt gcacagacaa
     6721 tacaaaaaaa aacatacaga agaatttttc cacttatccc attcaacttt tcgattttac
     6781 agagcgtcag ggcaagcctg ctatgctgaa caaacttatg gcatatgcta ctgaaaaaaa
     6841 tgcgattact atatttacgg atgcagacat ttttttcaat cagaatacac tttttgagtt
     6901 ggttaaatat tttaaggatg agcgtatcgg tttagtagat acgattattc aaacacaaag
     6961 aagcacggat aataacgaac agttttactg gaattacgaa aatgaaataa aatggaatga
     7021 atctttagtg tatggaaaaa ttactggtcc atcaggaggc tgttatgcta tcagaacaaa
     7081 gttatatgaa gaaattccgg taaattttgt tgttgatgat ttttttatcg ggatgaaaat
     7141 tgccctcaaa aaatatcata ctattgtaaa taaagaagca cttgcaattg aagaaatcca
     7201 aacaaactgg atacaagaat tcagaagaaa gagtagaata gcttcgggta atttccagaa
     7261 tttgtggtat ttcaaaaaga ctgcctttca agtctataca ccgctgggtt ttattttctt
     7321 ttctcataaa tttttgcgtt ggaaaacacc atttatatta ttgattttat attatttgct
     7381 gttatttaag gcaacattgc ttattctaat cgtaacattt attttgcctg tttttgatgc
     7441 tatttgcgct atttttggtt gggcaattaa gccactccga agattgcatt atttcctttt
     7501 tatgaatctt gcagtttttt ggggttttat taatttttgt aaaggaataa aatcaaatgt
     7561 ctggcaacca acaccacgca aataatatac agttagatac tattgaatcg gctgtagatg
     7621 ctattaaaaa tggcaaatta gtcattgtag tagatgatga agatcgtgaa aacgaaggcg
     7681 attttattac cgcagctcgc aatgctacac cggaaattat caatttcatg gctacgcatg
     7741 gcagaggctt aatttgcacg ccgcttatag aaagtagatg cgatgagtta aaactggaac
     7801 tcatggtaaa tgccaattca tctcactatg atactccgtt tactgtatcg gttgatttaa
     7861 tcggagaagg atgtacaaca ggcatttccg cttctgacag agctaagact attcaggcat
     7921 taatcaatcc
//
LOCUS       DAOQII010000101         7820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5068_length_7820_cov_5.262202, whole genome shotgun sequence.
ACCESSION   DAOQII010000101 DAOQII010000000
VERSION     DAOQII010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5068_length_7820_cov_5.262202"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLF48_06240"
     CDS             <1..629
                     /locus_tag="PLF48_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNF48961.1"
                     /translation="AIAVKKVNDPRKFGVAEFDDKGFITKTVEKPAIPKSNMAVVGIY
                     KIKEFNVLIQCLEANIKEQKRSNGQYTLTDGIQDMLEKGIQIAGFKVENWYDCGQKEI
                     LLETNRLMLNKQSKVAYNKKNIQNSILVEPVYLGKNAILKDSIIGPNVTIGENAVVQN
                     SIIRDSIIGSYTRLNEVILHQSLIGNDSEIWGSTQSLNIGDNTELDLR"
     gene            648..1952
                     /locus_tag="PLF48_06245"
     CDS             648..1952
                     /locus_tag="PLF48_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNF48962.1"
                     /translation="MFAIVDLETTGGNSSSERIIEIGIILHDGKQKIGEYTTLVNPEK
                     EISAFISTFTGITNAMVKHAPKFEDIAHTVAELLENNVFVAHNARFDYHFLRSEFKRL
                     NIPFSKKTLCTVQYTRKVFPHKKSYSLGNICKELEIPIDNRHRAFGDALATAYLLEKA
                     IANDKKDYLNDFLTDDIHKMYLPEKISIEQVDVLPEELGVIYFHNEQGEIVYIDKSKN
                     IREYVINFFLKKPIEKNKIDLHRIIAGISFEASGSELVASLQEFHEKMQHKPRYNRPV
                     SMQRMRFGLFAEDDAQGFQKLMVKLIEEAENPLLKFTSKFKADRMMHSILTSSRLEPA
                     FKKIESSAVYNRRLQEVLAKFIYPHNSFFIIDEGRGGTEKSIVKIENNEYKGFGFIEQ
                     AYIASMEDLHDAVQFREEFHDAKKVIQSYLRKNGKYLNIIPF"
     gene            complement(1939..2436)
                     /locus_tag="PLF48_06250"
     CDS             complement(1939..2436)
                     /locus_tag="PLF48_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48963.1"
                     /translation="MKTLSVNIYKSLFDIIWISISAIAAYLFLFNVQPIVSSDFFRFL
                     YLGIFLAFIYFGYTIFLFDSKLAQNIWVKVLWFTANIFLFFVLLKQYYTFAKVTDDYN
                     YTLASGQVIKPYTELDMQLYIKKMIILAGIIPLFLMIVFQIRLFISFFQTRQLDKVAD
                     VFRKV"
     gene            complement(2493..4439)
                     /gene="ftsH"
                     /locus_tag="PLF48_06255"
     CDS             complement(2493..4439)
                     /gene="ftsH"
                     /locus_tag="PLF48_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNF48964.1"
                     /translation="MDFLPEEDKNKNNNKKKGFNSYWIFALLFAIFLILQFFTMSVAS
                     RQTTEDYFFKKLVPSGDVDSIDIVNNDRVDIFINADSLQKKAIYKDIRTTKLGRLNIG
                     PHYSFDVINLNTFVEHLDETQKSLAYEQIIKKNRKKEGQYLGLFLQWVLPLLLIVGLW
                     MFLMRRASGGVGGPGGQIFNIGKSKASLYDKDTKVSVTFDDVAGLDEAKEEVKEVVDF
                     LKNPKKYTALGAKIPKGVLLVGPPGTGKTLLAKAMAGEAQVPFFSISGSDFVEMFVGV
                     GASRVRDLFKQAREKAPCIIFIDEIDAVGRARGKNMVQSNDERESTLNQLLVEMDGFS
                     GDKGIIMLAATNRPDVLDSALLRPGRFDRQISIDRPDIKGREQIFKVHLKKLTKLGPD
                     VNPEKLASLTPGFAGAEIANVCNEAALIAARKEKTQVELSDFNDAIDRTIGGLEKKNK
                     IISPQEKEIIAYHEAGHAVCGWYLEHAHPLLKVTIIPRGIAALGYAQYLPKEQYLYRT
                     EQLMDELCMTLGGRAAEDIVFGKISTGAQNDLERITKLCYAMVTIYGMNETLGNISYY
                     DPQDNGFTKPYSEETAREIDMQVRELINKAYERTKNLLTEKRHELDVIAKTLLKKEIL
                     FKSDLDELIGKRPFEEEPLPVSLV"
     gene            complement(4501..4884)
                     /gene="rsfS"
                     /locus_tag="PLF48_06260"
     CDS             complement(4501..4884)
                     /gene="rsfS"
                     /locus_tag="PLF48_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNF48965.1"
                     /translation="MEKTKIKRTKKKLTTADLHFLIVDLIQDRKGKEIVSLDLTQIPE
                     AITDYFVICHGESTVQTRAIIDHIEEELLKEHKLKPLHIEGRSFGEWCLIDYGDIVVH
                     VFQKEKREFYQLEDLWSDAVRKDYD"
     gene            4921..5763
                     /locus_tag="PLF48_06265"
     CDS             4921..5763
                     /locus_tag="PLF48_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNF48966.1"
                     /translation="MNILIQNTQFTGKQLLHLERVDSTNNYAKQVIANSTPKDGTVIL
                     ADEQFAGRGQAGNKWQSEAGKNLTFSILYHTSFLRADEQYYLNMAVSLGVRKAIEMEM
                     KEEYEPRIKNQELRSINEDIEKSLVADLFPVTIKWPNDIFAGEKKIAGILIENTIQGQ
                     FLNYSVIGIGINVNQTVFETGLSASSITLLNNKQADLVQLFKNALYLIEYYFLKLKMK
                     DYAAIKTAYMEHLMGYRQSLTYRKGQFLFEGSITDIDPAGKLMLQVGDYVQTFQFKEI
                     ERVF"
     gene            complement(5784..>7820)
                     /locus_tag="PLF48_06270"
     CDS             complement(5784..>7820)
                     /locus_tag="PLF48_06270"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNF48967.1"
                     /translation="VERPQHMLMRVAVGIHKEDIDAAIETYDLMSEKWFTHATPTLYN
                     AGTPKPQLSSCFLLTMKDDSIRGIYDTLRNCAEISQAAGGIGLSIHNIRAKGSYIKGT
                     GGESNGLVPMLQVFNSTARYVDQGGGKRKGAFAVYLEPWHADIFDFIELKKNHGKEEL
                     RARDLFYAMWIPDLFMKRVKDNASWSLMCPNECPGLCDVYGEEFEALYLKYEAEGKVR
                     KTIKAQELWFHIMDSQIETGTPYMLYKDHANRKSNQKNLGTIRSSNLCTEIMEYTSAD
                     EIAVCNLASINLSRFVDTENKAFDFQRLYDITYIVTKNLNKVIDINYYPVQEAKNSNM
                     RHRPIGLGVQGLADTFMMLRHAFDSEEAKILNKNIFETIYFAALSASKDLAKKDGAYQ
                     SFEGSPISQGIFQFDMWKVQPTDRWDWETLRSEIMEFGVRNSLLVAPMPTASTSQILG
                     NNECFEPYTSNLYTRRVLSGEFMVVNKHLLKDLIELELWSDEMKNAIIANKGSIQSIN
                     GIPQEIKELYKTAWEIKQRVILDMAADRGAFIDQSQSLNLFMESANYQKLTSAHFHAW
                     EKGLKTGMYYLRTRPAMDPIQFTVDMEKARKANDMVANDPSYKHTQQAVAPIYESVAN
                     AQQVMFEVNETAVKQDEMTIEQRAAEFGMTVEEFLQAQNACSLENPGACEMCGS"
BASE COUNT         2429 a         1546 c         1485 g         2360 t
ORIGIN      
        1 ctgctatcgc tgtaaaaaaa gtaaatgacc ctcgcaaatt tggtgtggcc gaatttgatg
       61 ataagggatt tattaccaaa actgtcgaaa agcccgccat ccccaagtcg aatatggctg
      121 ttgtcggtat ctataaaatc aaagaattta acgtgctcat tcaatgtctg gaagccaata
      181 tcaaagaaca aaaacgaagt aatgggcaat atacgctcac tgacggcatt caggatatgc
      241 tggaaaaggg catacaaatt gcaggattta aagtagaaaa ctggtacgat tgcgggcaaa
      301 aggaaatctt gctggaaacc aacagactga tgctgaataa gcagtcaaaa gtggcatata
      361 ataaaaagaa tattcaaaac tcaattttag tagagcccgt atatctcggg aaaaatgcca
      421 tactgaaaga ttcgataatc ggaccaaatg tgaccattgg agaaaatgcc gttgttcaaa
      481 attccatcat tcgcgattcc atcattggca gttatacccg attgaatgaa gtcatcttgc
      541 atcaatcgtt aattggcaat gattcggaaa tttggggcag tacgcagagc ttgaatattg
      601 gagataatac ggaactggat ttgagataaa attttaaaaa aaataaggtg tttgcaatag
      661 tagatttaga aaccacggga ggaaacagca gctcagagcg aattattgag attggcatta
      721 tcttgcatga cggcaaacaa aaaatcggag aatacaccac attagtcaat cccgaaaaag
      781 aaatcagtgc atttatttct acgttcacgg gtattacaaa cgccatggta aaacatgctc
      841 caaaatttga agatatcgca cataccgtag ctgaattgct ggaaaataat gttttcgtgg
      901 cacataatgc ccgttttgat tatcattttc tgcgcagcga attcaaacga ctgaatattc
      961 cctttagcaa aaaaacattg tgtacggtgc aatacacccg taaagttttc ccgcataaga
     1021 aatcatatag tttgggcaat atttgcaaag aactggaaat accgatagac aacagacatc
     1081 gtgcattcgg agatgctttg gctacggcat atctgcttga gaaagcaatt gcaaatgaca
     1141 aaaaagacta cttgaacgat tttttaacgg acgatattca taaaatgtat cttccggaaa
     1201 aaattagtat cgaacaagta gatgtattac cggaagaact gggggtgatt tactttcaca
     1261 atgaacaagg agaaatcgtc tatattgaca agagtaaaaa tatccgagag tatgtcatca
     1321 actttttcct caaaaagccc attgaaaaaa acaaaataga tttgcaccgc attattgccg
     1381 gcatcagttt cgaagccagt ggcagcgaat tagtcgccag cttgcaggaa tttcatgaaa
     1441 agatgcaaca taaaccgagg tataaccgcc cggtttccat gcaaagaatg cgatttggct
     1501 tgtttgcgga agatgatgca cagggttttc aaaagctgat ggtcaaacta attgaagaag
     1561 cggagaatcc attgctgaaa tttacgtcta agtttaaagc tgacagaatg atgcattcca
     1621 ttctgacttc atcccgcttg gaacctgcct ttaagaaaat tgaatcgagt gcagtgtata
     1681 accggcgttt acaagaggta ttggctaaat ttatttatcc gcacaacagc ttttttatta
     1741 tcgatgaagg cagaggaggc acagaaaaga gcatagtgaa aatagaaaac aatgagtaca
     1801 aaggtttcgg atttattgaa caagcctata tcgccagtat ggaagatttg catgatgccg
     1861 ttcaattccg tgaagaattt cacgatgcca aaaaagtgat acaaagttat ttgcgcaaaa
     1921 atggaaaata cctgaatatt atacctttct gaatacgtca gctactttat ccaactgacg
     1981 tgtttggaaa aaagaaataa ataaacggat ttgaaatacg atcatcaaaa agagcgggat
     2041 aattccggct aaaataatca ttttcttaat atacaattgc atatccagtt ctgtgtaagg
     2101 tttaattact tgtcctgaag caagggtgta attataatcg tcagttactt tggcaaaggt
     2161 gtaatattgt ttgagtaaaa caaaaaacaa gaaaatattt gccgtaaacc acaatacttt
     2221 cacccatata ttctgtgcga gtttcgagtc gaataaaaaa atggtatagc caaaataaat
     2281 aaaggccaga aaaataccga ggtacaaaaa gcggaaaaag tcactgctga cgatgggttg
     2341 cacattaaac aaaaacagat aagcagcaat tgcagaaata cttatccaaa tgatatcaaa
     2401 cagagatttg taaatattga cggagagtgt tttcatcgtg taaaggtaaa cagataaaat
     2461 caaacaaatg aatcaacttg tttctttggc aattacacca atgaaactgg aagcggctct
     2521 tcttcaaacg gtcgtttgcc tatcagttca tccaaatcag acttgaataa aatctctttt
     2581 ttcaataaag ttttggcaat tacatccaac tcgtgtcttt tttctgtcaa aaggtttttg
     2641 gttcgctcgt atgccttatt aattaattct cgcacttgca tatcaatttc tcttgccgtt
     2701 tcttcagaat atggcttggt gaaaccgtta tcctgaggat cgtaatacga gatatttccc
     2761 aaggtttcat tcatgccata aattgtaacc atggcatagc acaatttcgt gatgcgctct
     2821 aagtcatttt gagctcctgt agaaatctta ccgaatacta tatcttcagc agcacgtcca
     2881 cctaaagtca tacaaagttc atccattaat tgctcggttc ggtacaaata ttgttcttta
     2941 ggtaaatact gggcgtaacc aagtgccgca atgccacgcg gaatgatagt aactttcaat
     3001 aaaggatgcg catgttccag ataccagcca caaacagcat gtccagcctc atgataagca
     3061 atgatttctt tttcttgagg agaaataatc ttgtttttct tttccagtcc gccaatagtt
     3121 ctgtctatgg catcattgaa atcagaaagt tccacttgtg ttttttcttt acgggcagct
     3181 atcaatgcag cttcattaca cacgttggca atttctgctc cggcaaatcc gggagtaagc
     3241 gaggctaatt tttcgggatt aacatccgga cctaattttg tcagtttttt caaatgaact
     3301 ttaaaaatct gctctctgcc ttttatgtca ggtcggtcaa tagatatttg tcggtcaaat
     3361 cgtccggggc gcaacaaagc agagtccagt acatctgggc ggttggttgc agccagcatg
     3421 ataatgcctt tatctccgct gaaaccatcc atctctacca gcaactgatt cagggtgctt
     3481 tcccgttcat cattgctttg taccatgttt ttgcctcggg cacgacctac tgcatcaatc
     3541 tcgtctataa aaatgataca aggagctttt tcccgggctt gtttaaacaa atcacgaaca
     3601 cgtgatgcac ctacacctac aaacatttct acgaaatcgg aaccggaaat tgagaagaaa
     3661 ggcacttgtg cttcaccagc catggcttta gccagcaagg ttttaccggt tcccggaggg
     3721 cctaccaaca gtacgccttt cggtatttta gcacccagag ccgtatattt tttgggattt
     3781 ttcagaaaat ccactacttc ttttacttct tctttcgcct catccaatcc ggctacatca
     3841 tcaaatgtta cacttacttt agtgtctttg tcgtacaaag aagctttact tttaccgatg
     3901 ttgaaaattt gtccgccggg gccacccaca cctcccgaag ctctgcgcat caaaaacatc
     3961 cacaatccta ctatcagcaa gagcggtaat acccattgca aaaacagtcc caagtattga
     4021 ccttcttttt tgcggttttt cttgatgatt tgctcgtatg ccagactctt ctgtgtctca
     4081 tcaaggtgtt caacaaaagt gttcaggtta attacatcga atgagtaatg aggaccgata
     4141 ttcaaacgac ctaattttgt ggttcggata tctttgtaaa ttgctttttt ctgcaaagaa
     4201 tctgcgttga tgaaaatgtc aacgcggtca ttgtttacga tatcaatact gtcaacatcc
     4261 ccggatggca ccagtttttt gaaaaaataa tcttctgtag tttgcctgct ggccacactc
     4321 atggtgaaaa actgcaaaat cagaaaaatt gcaaatagta aagcaaatat ccaatagctg
     4381 ttaaagcctt tctttttatt gttgtttttg tttttatctt cttccggtaa aaaatccata
     4441 gcgtattctt aagcttaaat gtattaaaat cgttgggtat gtgttttatg agatgttgaa
     4501 ttaatcgtaa tcttttcgca cagcatcact ccataaatcc tctaattgat aaaattcccg
     4561 tttttctttt tggaaaacat gaacgacaat atcaccatag tctatcagac accattcgcc
     4621 aaagctccga ccttctatat gcagtggctt cagtttatgc tctttcagca gttcttcttc
     4681 aatatgatca ataatagcgc gtgtttgcac ggtagactca ccatggcaga tgacaaaata
     4741 atctgtgatg gcttcgggta tctgcgtcaa atccaaactt acaatttctt tcccctttct
     4801 gtcttgaatc aaatctacaa tcaaaaaatg aaggtcagct gttgttaatt tctttttagt
     4861 gcgtttaatt tttgtttttt ccaaatctgt ttacttattt tatctcaaat ctactaaatt
     4921 ttgaacattc ttattcaaaa tacacaattt acgggtaagc aactgcttca cctggagcgg
     4981 gtagattcta ccaataatta tgcaaaacaa gtaatagcaa atagcacacc aaaagacgga
     5041 actgttattt tggcagacga acagtttgcc ggacgtggtc aagccgggaa taaatggcag
     5101 agtgaagccg gaaaaaatct cactttttct attctgtacc atacttcatt tttgagagct
     5161 gatgagcagt attatttgaa tatggctgtt tcgcttggtg tgagaaaagc tattgaaatg
     5221 gaaatgaagg aagaatatga accaagaatc aagaaccaag aattaagaag tataaatgag
     5281 gatatagaaa aaagccttgt tgcagattta ttccctgtta cgattaaatg gccgaatgat
     5341 atttttgcag gggaaaagaa aattgcaggt atcttgatag aaaataccat acaagggcaa
     5401 tttctgaatt attcggttat tgggattggt atcaatgtaa atcaaactgt ttttgaaaca
     5461 ggattaagcg caagcagtat tactttgctc aataataaac aagcagattt agtacagctg
     5521 tttaaaaatg ccctttatct catagaatat tattttctta aactaaaaat gaaagactat
     5581 gctgccataa aaacagcata tatggaacat ttgatgggct acagacaaag cttgacttac
     5641 cgcaagggac aatttttatt tgaaggaagc attacagaca tagaccctgc aggaaagtta
     5701 atgctgcaag ttggtgatta tgtacaaact ttccaattta aagaaattga aagggtattt
     5761 tagctgattt tgaatgtatg agtttatgaa ccacacatct cgcaagctcc cggattttcc
     5821 agtgaacatg cattttgtgc ctgcaaaaat tcctcaacag tcattccaaa ctccgctgca
     5881 cgttgttcaa ttgtcatttc atcctgtttt acagcggttt cattcacttc aaacataact
     5941 tgctgggcat tagctacgct ttcatatatc ggagctacag cttgttgggt gtgcttatag
     6001 cttggatcat tggcaaccat gtcattggct ttgcgtgctt tttccatatc gacagtaaac
     6061 tgtatcggat ccatggcagg gcgggtacgc aaataataca tgcctgtttt tagtcctttt
     6121 tcccatgcat ggaaatgagc ggaagtcagc ttttggtagt ttgcgctctc cataaataaa
     6181 ttcaaacttt ggctttggtc gataaatgcg ccacggtcgg cagccatgtc taaaataaca
     6241 cgttgtttga tttcccaagc tgttttataa agctctttta tttcctgagg aatgccattt
     6301 atagattgaa ttgatccttt attggcaatg atggcatttt tcatctcatc actccacaat
     6361 tctaattcaa ttaagtcttt caaaagatgc ttatttacta ccataaactc gccgctcaat
     6421 actctgcgag tatataagtt ggaggtgtac ggttcaaaac attcattatt gcccaaaatc
     6481 tgagaagtag aagctgttgg cataggtgca accaataagc tgttgcgaac accaaattcc
     6541 attatctcac tgcgaagagt ttcccagtcc caacgatcag taggctgcac cttccacatg
     6601 tcaaactgga agatgccctg agaaatcgga gaaccttcaa atgactgata agcaccgtct
     6661 ttttttgcta aatcctttga agcacttaat gcagcaaaat agatagtctc aaatatgttt
     6721 ttgttgagga tttttgcttc ttcgctatcg aatgcatgtc gcagcatcat gaaagtatcc
     6781 gctaatcctt gcacacccaa accaattgga cgatgacgca tattgctgtt cttagcttcc
     6841 tgcacaggat aatagttgat atcaattact ttattcagat ttttagtaac gatataagta
     6901 atatcataca gacgctggaa atcaaaagcc ttgttttcgg tgtcaacaaa gcgactcaag
     6961 tttatcgaag cgagattaca tactgcaatt tcgtcagctg acgtgtactc cataatctcc
     7021 gtacataaat tgcttgaacg aatagtacct aagttctttt ggttagattt gcggttggca
     7081 tggtctttat aaagcatata aggcgtaccg gtttcaattt ggctgtccat gatatgaaac
     7141 cacaattctt gtgcttttat tgtttttctt accttgcctt ctgcttcgta ttttaaataa
     7201 agcgcttcaa attcttcacc atatacatca cataatccgg ggcactcatt tggacacatg
     7261 agcgaccagc tggcattgtc tttcacccgt ttcataaata gatccggaat ccacatggca
     7321 taaaataaat cgcgggcacg cagctcttct ttgccgtgat tctttttcaa ttctataaaa
     7381 tcaaaaatat cagcatgcca cggctctaag taaacggcaa atgctccttt gcgtttgcca
     7441 ccgccttggt ctacataacg ggctgtggaa ttaaatactt gcaacatcgg cactaagccg
     7501 ttgctctcac caccggttcc ttttatatag cttccttttg ctcttatatt atgtatgctc
     7561 agcccgattc cacctgcagc ttgcgaaatt tctgcacagt tgcgcaaggt atcatatatg
     7621 ccgcgaatgc tgtcatcttt cattgtcagc aaaaagcaag aagaaagttg tggcttaggc
     7681 gtgccagcat tatagagtgt aggcgtggca tgggtaaacc atttttcaga cattaaatca
     7741 taggtctcaa tggcggcatc aatatcctct ttatgtatgc ctacggctac gcgcatgagc
     7801 atgtgttgtg ggcgttccac
//
LOCUS       DAOQII010000102         7814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5076_length_7814_cov_3.614254, whole genome shotgun sequence.
ACCESSION   DAOQII010000102 DAOQII010000000
VERSION     DAOQII010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_7814_cov_3.614254"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLF48_06275"
     CDS             <1..802
                     /locus_tag="PLF48_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48968.1"
                     /translation="EHYLKNPASLAPKIFVVPLTISYPFVLEASSLIEEHLKRTGKEQ
                     YLVKDVPTIPGKAIWKFFWETFSKSSDITLSLSEPMDVFGNHLDDDGNSIDKSGRPVE
                     IRQYFFNNDQLKIDDQRESIYTQMLGDKIVRKFHENNVVYSSHVVAFVAFELLRKQLN
                     YPDIYTMLRTPEEEREINADDLKMNVKRILDELYNLNNKGKIKLSPNLITKEIHEIID
                     RGVDQMSIYHANIPLMKMKNGNYTSEDLKLLYYYHNRLEGYGLHKYIG"
     gene            777..1838
                     /locus_tag="PLF48_06280"
     CDS             777..1838
                     /locus_tag="PLF48_06280"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNF48969.1"
                     /translation="MAYTNTSDKVKACVIGAGSFGTVVANLLAENGDVLIYSRRQDAI
                     DSINKDKLNNGQQMHQNVRATHDLQEITESSYVLFPSVASDAFRPLMREMSPLLKPNH
                     ILIHCTKGFDIKLEEGQSLLDKDLKLRPADIYTMSKVILEETIVKRVGCMSGPNLARE
                     IASMHPAATVIASKFDEVIREGENAIRSKRLQVYGNHDIYAVELAGVLKNSMALAAGA
                     LGGLGYGNNAMAFLITRGLGEIIRLATAMGAEKQAFLGLAGIGDLIATCTSPLSRNYT
                     VGSRLAKGETLEQIISTSSEIAEGIKTVSISKKIADACDVRTPIIQQTYKVLFEGLKV
                     EEALQRLMEYRWGSDADYM"
     gene            complement(1958..2779)
                     /locus_tag="PLF48_06285"
     CDS             complement(1958..2779)
                     /locus_tag="PLF48_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF48970.1"
                     /translation="MTTFKNKTVFISGGTRGIGHAIGLRLAREGANIVIAAKTTEPQP
                     KLPGTIYTAAQDMENAGGKGLAIKCDIRSEEEVIAAVQKTVETFGGIDILINNASAIN
                     LSPTLFLEMKRYDLMQDINTRGTFLCSQKCIPHLLKAENPHILNLSPPLTSITEHWFK
                     NHVAYSIAKFGMSLCVLGMSGEFKGKIGVNALWPRTTIATAAVQNLLGGDSIINASRK
                     PEILADAAYFILSRDAKTCTGNYFMDDEVLQSEGITDLEQYAVKPGTPLTPDFFV"
     gene            complement(3001..>4716)
                     /locus_tag="PLF48_06290"
     CDS             complement(3001..>4716)
                     /locus_tag="PLF48_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF48971.1"
                     /translation="SEEQKMIGDMVKDFCVKELQGLGIERVAQMDASKDMDTIKDMFH
                     KASELGLCGVSISEEYGGMGLDFNTGLIFSENIALGLSFATTIGAQTSIGSLPIVYYG
                     TKEQKAKYLPKIASGEFVCSYCLTEPTAGSDANSGKTKAVLNEAGTHYLINGQKMWIT
                     NGGFADVFIVFAKIDNDEKLSAFIVEKDFGGIEIGKEEKKMGIKASSTVQLFFTNCPV
                     PVENLLGERQAGFKMALNILNSGRIKIGAGAVGGLKFALERSSEYATQRVQFGKPIAD
                     FGAIKTKIGKIGAEAFAIESAVYRTGADIDKKYNELTAAGDNENEAKINAIREYAIEC
                     SILKVIGSESICNAADEAIQIFGGMGYSMETGVEMAYRDARITKIYEGTNEINRMLIL
                     AEFYKRAFQTKEIKMNDAMKTIPLGIAQNFNPFNSGFLAEESEIVSNMKSVFMIITGA
                     AGRKLKAKLVDEQEIVMNLADLLSYAYLCESALLRVQKLKEQNHDKDALAIKIKMTQS
                     FIYDALEHCRNAANEAIDSYANGVEKFTIRRVVDGLLKNYDINPKDTRRAIADAVYAA
                     NGYPA"
     assembly_gap    4717..4816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5085..6620)
                     /locus_tag="PLF48_06295"
     CDS             complement(5085..6620)
                     /locus_tag="PLF48_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNF48972.1"
                     /translation="MLVNTYGSAVFGVDAYIITVEVNVCQGSKYYLVGLPDNAVKESW
                     SRVESALRANKYNMPRIKIIVNLAPADIRKEGSAYDLPIAIGILSASEQLFTDMLDKY
                     IIMGELSLDGKLRPIKGALPIAIKAKQEGFEGIIVPRENAREAAIVSGIKVFGLDHID
                     EVVNLLSGKTSFEPLQINTRDEFVFNSAMATIDFEDVKGQENIKRALEISAAGGHNVI
                     LIGPPGAGKTMLAKRLPTILPPLTLHEALETTKIHSVAGRLKSNASLLYERPFRAPHH
                     TVSDVALVGGGGNPQPGEISLAHNGVLFLDELPEFKRSVLEVMRQPMEERKVNISRAK
                     FSIDYPANFMLIASMNPCPCGFYNHPEKECVCAPGVVQKYLNRISGPLLDRIDLHVEV
                     TPVNINELSSVKKSEKSCVIRERVIQARNIQLERFNTHEIYCNAQMTTKMTNKYCQLD
                     ATSQKLLSTAMDKLGLSARAYERIIRTARTIADLDSCIDIQTHHIAEAIQFRSLDRSS
                     WAG"
     gene            complement(6991..>7814)
                     /locus_tag="PLF48_06300"
     CDS             complement(6991..>7814)
                     /locus_tag="PLF48_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48973.1"
                     /translation="RLSWCPVVTVKVFSDKRNESIFSYSTYDKVLDSIESSEHKEVND
                     NSKFLLPYNKDATLYFGYYKSKKDNGPSRQDINYLMQKRDSFYINKGEIFNSERSYTY
                     DSSYMINGKFIQYKVIKNPAPRSYADLSDGIWYYFMVLNKSLYPMFKMEIKNKYLFNY
                     LNIYYINKQLMIQQHFTHGVNDTSRGYYENGNISYYELVDIYDNRTIQSIRYDSIGNI
                     YSYYDSICKCYIDYINGKLSTFQQYDSQEKILYSILFNEDLEIIEKKIYPKFKPK"
BASE COUNT         2306 a         1462 c         1411 g         2535 t
ORIGIN      
        1 cgaacattac ttaaaaaatc cggcatcgct tgcacctaaa atatttgtcg taccactcac
       61 catcagctat ccatttgttt tagaggcttc atcattgata gaagagcatc tgaaacgcac
      121 cgggaaagaa caatatctag ttaaagatgt tccaacaata cccggtaaag caatctggaa
      181 gtttttctgg gaaacatttt ccaaatcgag tgatattacc ttgtctttga gtgagccaat
      241 ggatgtattc ggcaatcatt tagatgatga tggtaacagc atagataaat caggcagacc
      301 agtcgaaatc cgacagtatt tttttaataa tgaccagctc aaaattgacg accaacgcga
      361 gagtatttat acacaaatgc tcggagacaa aattgtgcga aaatttcatg aaaataatgt
      421 tgtttattct tcacatgttg tcgcatttgt cgcattcgaa ttgttgcgca aacagctcaa
      481 ttacccggat atttatacga tgctgagaac gcctgaagaa gaaagagaaa ttaatgctga
      541 tgatttgaag atgaacgtaa aacgcatttt agatgaattg tataatctta ataataaagg
      601 aaaaattaaa ctttcaccaa acttaattac taaagaaata cacgaaataa ttgatagagg
      661 tgttgaccaa atgagcattt atcatgcaaa tattcctttg atgaaaatga aaaacggcaa
      721 ctataccagc gaagatttga aattattata ttactaccac aacagactgg aaggctatgg
      781 cttacacaaa tacatcggat aaagtaaaag catgtgtaat aggcgcaggc agtttcggta
      841 ctgtagtggc taatttattg gcagaaaatg gagatgtttt aatctattca cgcagacagg
      901 atgccataga cagtatcaat aaagacaaat tgaataacgg acaacaaatg caccagaatg
      961 tgcgggcaac ccatgatttg caagaaatta ctgaaagcag ttatgtattg tttccctcag
     1021 tggcatctga tgcattcagg cctttgatgc gcgaaatgtc gcctttgcta aaacctaatc
     1081 atatccttat tcattgcaca aaaggatttg atataaaatt ggaagaggga caaagcttgt
     1141 tggataaaga tttaaaactt cgtccggcag atatatatac gatgagcaaa gtaattttgg
     1201 aagaaaccat cgtaaaacgt gtgggctgca tgagcggacc caatctggcc cgggaaattg
     1261 ccagtatgca tccggcagcc accgtaatag ccagcaaatt tgatgaggta atccgtgagg
     1321 gcgaaaatgc catacgcagc aaacgattac aagtgtatgg caaccatgat atttatgccg
     1381 tcgagcttgc cggtgtactt aaaaactcga tggcattagc agcaggtgct ttaggcggct
     1441 tgggttatgg caataatgcg atggcctttt tgattacgag aggattgggc gaaatcatca
     1501 ggctggctac agccatgggc gcagaaaagc aagcatttct gggtttggcc ggcatcggag
     1561 atttaatagc gacctgcaca tcacctttaa gcagaaacta taccgttggt tcgagattgg
     1621 cgaaaggaga aaccttggag cagattatat ctacctcttc tgaaattgca gaaggcataa
     1681 aaacggtgag tatcagtaag aaaatagcag atgcatgcga tgtacgaacg cccatcatac
     1741 aacaaacata caaggtgctg tttgaaggat taaaggtaga agaagccttg caaaggctga
     1801 tggaataccg ctggggcagc gatgccgatt atatgtaggt atttattatt tatatgtatt
     1861 taataaattt agtaaaagtt gttagtcaac aattttttat aattagaggg gcttttgttt
     1921 ttttttgatt aaaaagatta atataagtat atgaagatta aacaaaaaaa tcaggggtaa
     1981 gcggcgtgcc cggttttaca gcatattgct ccaaatctgt aatgccttca ctttgtaaaa
     2041 cttcatcatc cataaaataa tttccggtac aggttttagc atctctgctc agaataaaat
     2101 aggcggcatc tgccaatatc tccggcttgc gagaggcatt gatgattgaa tcgcctccca
     2161 ataaattctg aacggctgcc gtggctatgg ttgtacgcgg ccaaagtgcg ttaacgccaa
     2221 ttttaccttt aaattctcca ctcataccca aaacacataa gctcatacca aatttagcta
     2281 tagaataggc aacatgattt ttaaaccaat gctcggtgat ggaagttaaa ggtggcgata
     2341 aatttaagat atgcgggttt tcagctttca atagatgcgg gatgcacttt tgcgaacata
     2401 aaaatgtacc tctggtattg atatcctgca tcaaatcata gcgtttcatt tctaaaaaca
     2461 acgtaggtga gaggttgata gccgatgcat tattgattaa aatatcaata ccaccaaaag
     2521 tttctaccgt tttttgaaca gcagcaatta cttcttcttc acttcggata tcgcacttta
     2581 tggctaagcc ttttccgccg gcattttcca tatcctgtgc agctgtgtat attgtacccg
     2641 gtaattttgg ttgtggttcg gtcgttttag cagcaattac aatatttgcg ccttctcgag
     2701 ctaatcgcaa acctatagca tgaccgatgc ctctggtgcc accggaaatg aaaacagttt
     2761 tatttttgaa tgttgtcatt atttttttga ttggttgttg gttattagag attggtgatt
     2821 ggatattagt tattggttat tggttattgg ttattggtta ttagagattg gtcattggtc
     2881 attggttatt ggataatagt tgttagttat tagtttttgg ttatcactta tcacttatca
     2941 cttaatactt actacttaat acttactact tactacttac tactcactac ccactaccca
     3001 ctacgcagga tatccgtttg ctgcataaac tgcatctgct attgctcttc gtgtatcttt
     3061 aggattgata tcgtagtttt tcaataagcc gtcaacgaca cgtctgatgg taaatttttc
     3121 tacaccgttt gcataactgt ctatcgcctc attggctgca tttctgcaat gttccaaggc
     3181 atcataaatg aaagattgtg tcatttttat tttgatggct aatgcgtctt tgtcatgatt
     3241 ttgttctttc agtttttgaa cacgcaataa agcagattca cacaaataag cataagagag
     3301 taaatcagct aaattcatca caatttcctg ttcatcgacc aatttggctt tcaattttct
     3361 acctgcagct ccggtaataa tcataaatac acttttcatg ttagatacaa tctcactttc
     3421 ttctgcaaga aaaccggaat taaacggatt gaaattttga gcgatgccaa gaggtattgt
     3481 tttcatggca tcattcattt ttatttcttt ggtctgaaat gcacgtttat aaaattcagc
     3541 taaaatgagc atgcggttaa tctcatttgt gccttcgtag attttagtaa ttcgggcatc
     3601 gcgataggcc atttcaactc cggtttccat agaatatccc atgccaccaa atatctgaat
     3661 ggcttcatct gctgcattac atatcgattc ggagcctatt acttttagga tagaacactc
     3721 aatggcatat tctcgtattg cgttgatttt tgcttcattt tcattatctc cggcagcagt
     3781 aagttcgttg tattttttat cgatatccgc tcccgttctg taaaccgctg attcgatggc
     3841 aaaagcttca gcgccaattt ttcctatttt agtttttata gcaccaaaat cagctatagg
     3901 tttgccgaat tgtacacgtt gagtagcgta ttctgatgat ctttctaatg cgaatttaag
     3961 acctcctaca gcaccggcac caatttttat acgtccgctg ttcaggatat ttaatgccat
     4021 tttgaaacct gcttgtcttt cacccaatag gttttctaca ggaacagggc aattggtaaa
     4081 aaacaattgt acagtagatg atgctttgat acccattttc ttttcttcct tgccaatttc
     4141 tattccaccg aaatcttttt ctacaataaa agcagataat ttctcatcgt tatctatttt
     4201 ggcaaataca ataaacacat cagcaaaccc accattggta atccacattt tttgtccgtt
     4261 aataaggtaa tgtgtaccgg cttcattaag tactgctttc gtctttccgc tattagcatc
     4321 agagccggca gttggctctg tcaagcaata agaacaaaca aactcgcctg atgcaatttt
     4381 aggtaaatat tttgcttttt gttctttagt gccataatac acgataggca aggagccgat
     4441 ggaagtttgt gcacctatag tagttgcaaa agataatccc aaggcaatat tttcactgaa
     4501 tattaagccg gtattaaaat ccaaacccat gccaccgtac tcttcactta ttgatacacc
     4561 gcacaagccc agttcggagg ccttatgaaa catatctttg atggtgtcca tatccttgga
     4621 tgcatccatt tgtgccacgc gttctatacc aagaccttgc agttctttta cacaaaaatc
     4681 cttgaccata tcgccaatca ttttttgctc ttcagannnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnngttc ttttacacaa aaatccttga ccatatcgcc aatcattttt
     4861 tgctcttcag agaaatcttc cggtgtaaaa acttcgctta ccggagtgtt ggaaaagaga
     4921 aaatttcctc caattaattt tgatgcttgt gcaggcataa cttagttttt tgtaaaaata
     4981 aatcaaaaaa tccaatgcat gcattggatt aatgataact taatttaaag ggtttgttaa
     5041 ttaacaaagg taaattttgc atttaacttg aaattcggat gtttttatcc agcccaacta
     5101 cttcggtcta aacttctgaa ttggatagct tcggcaatat gatgtgtttg aatatcgata
     5161 caactatcca aatctgctat agtacgagct gtgcgaataa tacgttcgta agctcttgca
     5221 ctcaatccca atttatccat tgccgtggat agcagctttt gtgaagtagc atctaactgg
     5281 caatatttat ttgtcatttt ggtggtcatt tgtgcattac aataaatttc atgagtgttg
     5341 aaccgctcta attgtatatt tcttgcctgt ataactcgct ctctgattac acagcttttt
     5401 tcagatttct taactgatga aagttcattg atattgacag gagtaacttc cacgtgcaaa
     5461 tcaattctgt cgagtaaggg accggaaatg cggtttaaat atttttgtac aacacccggt
     5521 gcgcatacac attctttttc agggtggttg taaaatccgc aaggacaagg attcatgctg
     5581 gcaatgagca taaagtttgc cggataatca atactgaact tagcacggga aatattgact
     5641 tttcgttctt ccatcggttg ccgcatcact tccagcacac tgcgtttaaa ctcaggcaat
     5701 tcatccaaaa acaatactcc attgtgtgcc agcgaaattt cgccgggttg tggattgcca
     5761 ccgcctccca ccaatgccac atccgaaacg gtatgatgcg gtgcacgaaa cggacgttca
     5821 tacagcaaag aggcattaga tttcaatctg cctgccacgg agtgaatctt tgtcgtttct
     5881 aaagcttcgt gtaatgttag cggaggcaaa atggtaggca ggcgttttgc cagcatggtc
     5941 ttgccggctc ccggtgggcc gattaaaatt acattatgcc cgccagctgc cgaaatttct
     6001 aaggcacgtt taatattttc ctgtcctttt acgtcttcaa aatcaatggt agccatagcg
     6061 gaattaaaaa cgaattcatc ccgtgtattt atttgcaaag gttcaaagct tgttttgccc
     6121 gaaagcaggt tcactacttc atcaatatga tcaagcccaa atactttgat gccggatacg
     6181 atagcggctt cacgggcatt ttctctagga actatgatgc cttcaaagcc ttcttgtttt
     6241 gcttttatag ctatgggtag cgcaccttta atgggcctta atttgccatc cagagataat
     6301 tcccccatga taatatactt atccaacatg tccgtaaaga gttgttctga tgcagacagt
     6361 atgccgattg cgataggcaa atcgtaggca gaaccttctt tacgaatatc ggcaggagcg
     6421 aggttgacaa taattttaat gcggggcata ttgtatttat tggcacgaag tgcactttct
     6481 actctgctcc aactttcttt aacggcattg tcgggcaaac ctacaagata gtatttgctg
     6541 ccctgacaaa cattgacttc aacggtgatg atataggcat ctacaccaaa aactgcactt
     6601 ccatacgtat ttactaacat aaactgaata ttcctgacta tatagagtca ataaggatga
     6661 aaattccata aacaatcagt aatttttttg atttggaaat ttgataaagt agtagttttt
     6721 atatgccaca tagaggcgac acctttttat ttggttgggt ttggttattg actcacaccc
     6781 agcccctccg agaggagagt gttttgactt ggttgctcat ataatcttct tgctccaaac
     6841 aagccaccta tattcaggtt gttatttgag attgtaatta tgagcttttt caaagaactt
     6901 ttttagctga ttaaatttag tatcttggtt gtatattttc atttcccaac tctctatttc
     6961 gtttagttct tggtggtagc ttctcattta ttacttaggt ttaaactttg gataaatctt
     7021 cttttctatt atttccaagt cttcattaaa taatatgcta tatagtattt tttcttggct
     7081 atcatattgt tgaaaagttg acaattttcc attaatatag tctatataac atttacaaat
     7141 tgaatcataa taagaataaa tattcccaat agaatcgtaa cgaatagatt gaatcgttct
     7201 attatcgtat atatctacta gctcataata cgaaatattc ccattttcat agtaacctct
     7261 tgatgtatcg ttaactccat gagtaaaatg ctgttgaatc attaattgtt tgtttatata
     7321 gtatatattt aaataattga ataagtattt attcttaatc tccattttaa acattggata
     7381 taatgattta tttaaaacca taaaataata ccatatcccg tcagataaat ctgcgtacga
     7441 tctgggagca ggatttttga ttactttgta ttgtataaac ttaccattaa tcatatagct
     7501 gctatcataa gtatagctac gttctgaatt aaaaatttca cctttattta tataaaagct
     7561 atctcttttt tgcattaagt aatttatgtc ttgtcttgat ggtccattat ctttcttaga
     7621 cttataatat ccgaaatata gagttgcatc tttattatat ggaagtaaaa atttactatt
     7681 atcatttact tctttatgtt cactactttc tatactatcg agtactttat cgtatgtact
     7741 atatgagaag atactttcat ttcttttatc tgaaaatact tttacggtta caacagggca
     7801 ccaactcaat cttt
//
LOCUS       DAOQII010000103         7802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5090_length_7802_cov_4.482768, whole genome shotgun sequence.
ACCESSION   DAOQII010000103 DAOQII010000000
VERSION     DAOQII010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5090_length_7802_cov_4.482768"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLF48_06305"
     CDS             complement(<1..244)
                     /locus_tag="PLF48_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48974.1"
                     /translation="MKRLLLSILFITILTVSFVADNLSLACNMTMIEEVEEKPEKKVF
                     SYDCQHLFEIQTSQGHILVSSPFAFYSHIWWSDLPDI"
     gene            complement(349..837)
                     /locus_tag="PLF48_06310"
     CDS             complement(349..837)
                     /locus_tag="PLF48_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF48975.1"
                     /translation="MRKTFFMLAIMISAFSKAGNMFTVTNNELVLPNVIGFDAHAYDV
                     KSENDPMLDYVAEYLLEHPNVTKFVIEGHAFTEKNASDNMKLSLQRAAMVSYYLTERG
                     VSCDRLAVIAYGENKPIAPTDECNLLVNTRINFINLEFNNKPVQNYHPDPVSTKEFNP
                     CD"
     gene            complement(919..2652)
                     /locus_tag="PLF48_06315"
     CDS             complement(919..2652)
                     /locus_tag="PLF48_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF48976.1"
                     /translation="MANLFRKKSIQSILKETEAYPDMMHEAGITLDEQAQMLNKSLTT
                     FDLTALGIAAIIGAGIFSTIGNACFDGGPAVVFLFIGIAIACGFSALCYAEFASRIPI
                     AGSAYTYSYAVFGELIAWIIGWDLLLEYAIGNITVAISWSGNFAAFLHQCGIDLPKYL
                     VTSFFEAKKFMPDLYASAPKILGIPFIINIPAFMINLFITALVYVGIKESKNFANGMV
                     IFKIAVIIVIIIVGAFYVTPANWSPFVPNGFGGVLKGVSAVFFAYIGFDAISTTAEEC
                     KNPQRDLPKGMFNSLILSTILYVAVALVLTGMVHYTQLKNEAFLANAFVQKGINWMGG
                     IIAFSALIATASVMLVFQIGQPRIWMSMSRDGLLPKKFAEIHPKFKTPSFSTIITGLI
                     VGIPSLFLDSSLVTDMCSIGTLFAFILVCGGIIILEQKEKKQVKLSAKTGTLDEDLLS
                     ESAAPMTPKFKVPYINGKWLIAGLTLAVLAIIFTNAAFLHSIFPVEAGFDVWKHQIPL
                     WLFLLVLIYTAYKTFMENWSLIPVLGFLSCGYLVSTLGIANWLRFFIWMAIGLICYFI
                     YGVNHSKLRNQ"
     gene            2780..4024
                     /locus_tag="PLF48_06320"
     CDS             2780..4024
                     /locus_tag="PLF48_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48977.1"
                     /translation="MRKAIISLFLCFALTAKASYILLPMDETQTNHLKAYGITYWVLT
                     KGTTADWLLNYRGGSFAFPYQKDFEKECLIRNVKYEVIADAAMSKILQDIAQPEVNMD
                     DVKLEKAPKVAVYSPKTNNPWDDAVTMVLTYAEIPYKVIYDEEILQDELHLYDWLHLH
                     HEDFTGQYGKFWSAYQNADWYRNQVNDYETRAAKLGYNKVSLEKLAVAQKIKDFVMGG
                     GFMFAMCSATDSYDIALAAAGVDICTQQFDHDAIDPNAQNKLDFSQTFAFQNFQISTN
                     PWEYEFSTIDSRANSMEPDYFTLFDYSAKWDQIPAMLTQDHERVIKGFMGQTTGFKKM
                     LIKPDVLVMGENKSISEAKYIHGILGKGTWTFYGGHDPEDYQHRVGDPPTDLNLHPNS
                     PGYRLILNNILFPAAKKKPQKT"
     gene            4205..5032
                     /locus_tag="PLF48_06325"
     CDS             4205..5032
                     /locus_tag="PLF48_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNF48978.1"
                     /translation="MKIMCCIYSKLLLILVLLTSCSNASQPKDKIPDYETFTIQSKQV
                     GEERVINVWIPDNYKTAKDSLPVMYMADGGINEDFPHIANTLSKLISENKIKPLILVG
                     IENTQRRRDLTGFTEVAKDKEIAPVVGGSDKFRAFIKDELFPEIIKRYRTTNEKSIIG
                     ESASGLFVMETFFLTPEMFDNYIAFDPSLWWNNHYLVRTAKEHLANFPISEKRIWFAS
                     SNAEDISPYTTELAEIFKTENKASIQWKFSNEPKEKHTTIFRATKEKAIIWTLNETK"
     gene            5173..5481
                     /locus_tag="PLF48_06330"
     CDS             5173..5481
                     /locus_tag="PLF48_06330"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNF48979.1"
                     /translation="MTNNKTFLIVNAIPNEEDMQSFQAYLSQIIVIFTKFGGSGMQRW
                     KTSEQIMGQGGIKAIAVIEFPSAQAIKDMMASDDFNALNELRKKAYKQEVDLMICETL
                     "
     gene            5589..6122
                     /locus_tag="PLF48_06335"
     CDS             5589..6122
                     /locus_tag="PLF48_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48980.1"
                     /translation="MKTFTLSFIAILLLCSLKPPKKAAPQNDILYSKYGGDVDKMMQD
                     MRRQDSMKYPFLYNLNDEAKNDSSTLTDEQTKILDYADELPAFPNGTAGLNEYILGYK
                     LNIPDSLSSDKANVEVKFYVNTTGTTQDPVVIKTTNAKFDAFTRWLIYDMPTWTPAKQ
                     SGKTVNCYVTLKITYGQ"
     gene            complement(6131..6589)
                     /locus_tag="PLF48_06340"
     CDS             complement(6131..6589)
                     /locus_tag="PLF48_06340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF48981.1"
                     /translation="MFEETIQLRVRYHDCDPLGIVYHGHYAKFFEIGRTEAMRKHGFS
                     YKQLEEEGYAMPVVEMNIKYFRPARYDELMNIKTTIADFPEKNMVFHSEIYNQDNQML
                     TSSETTFVFVKKESLRRCHVPHIVIDHLKPYFEEELKAMKKSKAPKKHTL"
     gene            complement(6589..7623)
                     /gene="mltG"
                     /locus_tag="PLF48_06345"
     CDS             complement(6589..7623)
                     /gene="mltG"
                     /locus_tag="PLF48_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNF48982.1"
                     /translation="MRKKLLWIALFCIITGLFIFLYFYKVAFADNVNNSSKQKYQTLL
                     IPTGANFDDVLYILKSNKVLKNSNTFIRVAKALKYDTHVKPGRYLLSNNAGNFTIVRK
                     LRAGAQDPVKIVFNNIQNKEQLAENIAKQIEASKSDLLKEFNNPVLLDSMNLTEDNFP
                     TIFLANTYEFYWNTSAKKFVERMLKEYNKFWTDRRKDKAQELGLSPTEATILASIVQK
                     ESNHYDEYPEIAEVYINRLHQNWPLQADPTVMYALNKLGIRRRVYYADLKIQSPYNTY
                     INKGLPPGPICLPERKSIEETLDANDSKYMYFCAKEDFSGYHVFAETLEQHEENAKRY
                     QQALNEKKVK"
BASE COUNT         2628 a         1448 c         1359 g         2367 t
ORIGIN      
        1 caatatctgg taaatcagac caccagatat gtgaataaaa tgcaaaaggc gatgatacca
       61 aaatgtgacc ttgactggtc tgaatttcga aaagatgttg acaatcataa gaaaaaactt
      121 tcttttccgg tttttcttct acttcttcta tcattgtcat attgcaagcc aaacttaaat
      181 tgtctgcaac aaatgataca gtaagaatag taatgaataa tatggaaaga agaagtcttt
      241 tcatttttgc aaagatacaa taaataaact tgaggtcgtg ttaaaattac ttaaccataa
      301 caaacatgcg tataagcacc taaaaataaa tcaattatag agcttgtctt aatcacaagg
      361 attaaattct ttagtactta caggatcagg atggtagttt tgaaccggtt tattgttaaa
      421 ctctaagtta ataaagttga tgcgtgtatt tactaataaa ttacattcat cagttggcgc
      481 aattggttta ttttcgccat aagcaattac agcaagtcgg tcgcaggaaa cccctctttc
      541 agtcagataa tagctaacca tagcggctct ttgtaaactt aatttcatat tatctgaagc
      601 atttttttcg gtaaatgcat gtccttctat cacaaatttc gtaacattcg gatgctccag
      661 taaatattcc gccacataat ccagcatagg atcattttca cttttcacat cataagcatg
      721 tgcatcaaag cctattacat tgggtaaaac aagttcattg ttagttacgg taaacatgtt
      781 tccggctttg gaaaaagctg aaatcataat tgccagcata aaaaatgttt ttctcatggg
      841 ttgtgtgttt agttggttag cttcaaatgt aaagcaacgc taatatatga ggtttatttg
      901 tgaaaaaatt cttaaaaatt actgatttcg caatttactg tggtttactc cataaataaa
      961 ataacaaatc agcccaattg ccatccagat aaaaaagcgc aaccagtttg caataccaag
     1021 ggtagaaacc aaatatccgc aagacaaaaa tccgagtacc ggtatcaaag accagttttc
     1081 cataaatgtt ttataagctg tataaattaa taccagaaga aataaccaaa gtggtatctg
     1141 gtgtttccat acatcaaaac ctgcttcaac aggaaagatg ctatgcagaa aagcagcatt
     1201 agtgaagata atagccaaca ctgccaaagt taatcctgca attaaccatt tgccgttgat
     1261 atagggtact ttaaacttgg gagtcatagg agctgcagat tcagataata aatcctcatc
     1321 taatgtccct gtcttggcgc ttaacttaac ttgttttttc tctttttgct ccaaaataat
     1381 aatacctcca caaaccagaa taaaggcaaa taaagttcct atactgcaca tatctgttac
     1441 taacgacgaa tctaaaaata aggatggaat gcctacgatt aatccggtta tgatagtaga
     1501 aaatgatgga gttttaaatt ttggatgaat ctcagcaaat ttctttggca acaaaccgtc
     1561 acggctcatg ctcatccaga tacgtggctg tccgatttga aaaacgagca tcacactggc
     1621 tgttgcaatc agagcactaa atgcaatgat accacccatc caatttatcc ctttttgtac
     1681 aaatgcattt gccagaaaag cttcattttt tagttgggta taatgcacca tgcctgtcaa
     1741 gaccaaggcc acagcaacat acaagattgt agataagatt aatgagttga acatcccctt
     1801 cggcaaatcc ctttgtggat ttttgcattc ttcagcggtc gtagaaatcg catcaaaacc
     1861 gatgtaggca aaaaacaccg ctgatactcc tttcagcaca ccgccaaatc cgttaggtac
     1921 aaatggcgac caatttgcag gtgtaacata aaacgcacca acaatgataa taacaatgat
     1981 aactgcaatt ttaaaaatca ccataccatt ggcaaaattt ttagactctt ttatacctac
     2041 ataaactaag gctgtaatga ataaattgat cataaatgcc ggtatattga taatgaacgg
     2101 tatacctaaa atcttaggtg ctgatgcata caaatccggc ataaattttt tcgcctcaaa
     2161 aaaactggtc actaaatact taggcaagtc tatgccacac tggtgcagaa aagccgcaaa
     2221 attgcccgac catgaaatag ccaccgtgat attgcctatg gcatactcca gcaacagatc
     2281 ccaaccgata atccaggcta tcagctcgcc aaaaacggca taagaataag tgtaggcact
     2341 gcctgcaata ggaatacgcg aagcgaactc agcataacat aaagcagaga aaccacaagc
     2401 gatggcaata ccgataaata aaaacaccac tgccggaccg ccgtcgaaac aggcattgcc
     2461 aatggtagaa aaaatgccag ctcctataat ggcagcaatt cccaaagcag tcaaatcaaa
     2521 agttgtcaac gatttattca gcatttgcgc ctgttcatcc aaggttatac cagcctcgtg
     2581 catcatatcc ggataagctt ccgtttcttt cagaatggat tgaattgact tttttctgaa
     2641 taagttcgcc ataagttcgt tttgataaag ataaactatt ttttatatgt caaggtatag
     2701 caaacttagt gaagcactat tttctaattt aacaaaagac aacaaaactc ggaatcaaat
     2761 ttgatgtata tttgatgcaa tgcgaaaagc aataatcagc ctttttttat gttttgctct
     2821 aacagcaaaa gcctcttata ttttattgcc tatggatgaa actcaaacga accatcttaa
     2881 ggcatacggt attacgtatt gggtgcttac taaaggaaca accgccgact ggttgctcaa
     2941 ttaccgcggt ggaagttttg catttcctta ccaaaaagat tttgaaaaag aatgtctcat
     3001 ccgcaatgta aaatatgaag tgatagcaga tgctgccatg agtaaaatct tgcaggatat
     3061 tgcacaaccg gaagtgaata tggatgacgt aaaactcgaa aaagcaccta aagttgccgt
     3121 ttattcaccc aaaacaaata atccctggga tgatgccgtt acaatggtat tgacatatgc
     3181 agaaattcct tacaaagtaa tttatgatga agaaattttg caggatgagt tgcatctgta
     3241 tgattggttg catctgcacc atgaagattt taccgggcaa tacggaaaat tctggtcagc
     3301 ctatcaaaat gcagactggt acagaaatca ggttaatgac tatgaaaccc gagctgcaaa
     3361 attgggctat aacaaagtat cactggaaaa attagcagtt gcacagaaaa taaaggattt
     3421 tgtgatgggt ggtggtttta tgtttgccat gtgtagtgcc acagacagct atgatattgc
     3481 attggcagcg gccggagttg atatttgtac ccaacaattt gaccatgatg ccatagaccc
     3541 gaatgcgcaa aataaacttg atttttcaca aacctttgcc tttcagaatt tccaaatcag
     3601 taccaatcca tgggagtacg aattttccac catcgactcc agagccaatt cgatggagcc
     3661 tgattatttc accttgttcg actactcagc aaaatgggat caaatacctg ccatgcttac
     3721 tcaagaccat gaacgagtga ttaaaggatt tatggggcaa accactgggt ttaagaaaat
     3781 gctgattaag ccggatgtat tagtaatggg tgaaaacaaa tctatcagtg aggcaaaata
     3841 tatacatgga attttaggaa aaggcacctg gacattttac gggggccacg acccggaaga
     3901 ctatcagcac cgtgtaggcg atccgccgac cgatttgaat ttgcatccga attcacccgg
     3961 ctaccggctt atattgaaca atattttgtt tccggcagcc aagaaaaaac cacagaaaac
     4021 ataggaagtg ttttatcatc aaaaaaattg cacttaacac ttaaacatgg gtattatttt
     4081 caataatttt aatatatatt acttttatat tataatttac ataaacgatt agttttatac
     4141 aaaaattgaa tgtatcttag ttgctttcac gagttctaat gaaaaaaaat cacaacttca
     4201 atcaatgaaa attatgtgtt gcatttattc caaattactt cttatacttg ttttgctgac
     4261 aagttgttca aatgcatcac aacctaaaga caaaattcct gattacgaaa cttttaccat
     4321 ccaatctaaa caagttggag aagagcgagt aatcaatgtt tggatacctg acaattacaa
     4381 aacagccaaa gactccttgc ctgtgatgta tatggcagat ggtggaatta atgaagactt
     4441 tcctcacatt gccaatacct tgtctaaact tatcagtgaa aacaaaatta aacctttaat
     4501 tctcgtgggt atagaaaata ctcaacgaag aagggacttg acaggattta cagaagtagc
     4561 gaaagacaaa gaaattgcac cggtagttgg gggctctgac aaattcagag cttttattaa
     4621 agacgaactt tttcccgaaa tcattaaacg atacagaaca acaaacgaaa aaagtattat
     4681 tggagaatct gcatcagggc tttttgttat ggaaaccttt ttcctcactc cggaaatgtt
     4741 tgacaattac attgcatttg acccttcgct ttggtggaat aatcattatt tagtaagaac
     4801 agctaaagaa cacttagcaa atttcccaat ttcagaaaaa agaatttggt ttgccagttc
     4861 aaatgctgag gatatttctc catatacaac ggaattagct gaaattttta aaacagaaaa
     4921 taaggccagc atacagtgga aattttcaaa tgaacccaaa gaaaaacata ccaccatttt
     4981 cagggctaca aaagaaaaag ccatcatctg gacattaaat gaaacaaaat gaaacattta
     5041 gatagggaat atcagtaatt ctacttagat tttgtgattc tttgttattt ttagatacaa
     5101 aaaaagaatt tcaacgacta cccaactctt taaaatagca atatatttat aaattaaaat
     5161 agattaaaca ccatgacaaa caacaaaaca tttttaattg taaacgcaat acctaatgaa
     5221 gaagacatgc aaagttttca ggcatacttg tctcaaataa ttgtcatttt tacaaagttt
     5281 ggcggtagtg gaatgcagcg atggaaaaca tcagaacaaa ttatgggaca aggtggtata
     5341 aaagctattg ctgtcattga atttccttca gcgcaagcca taaaagacat gatggccagc
     5401 gacgatttca atgcactaaa cgagcttcgc aaaaaggcat acaaacaaga agtagattta
     5461 atgatttgcg aaacactgta agaaatattt atatctttga aaaacaaatt tatattattt
     5521 atcctttttt gttttttttc aaatatattt caccgatttt ttagcccgat ttttgtgtat
     5581 atttaagtat gaaaactttt acgctttctt ttattgccat ccttttgctt tgcagcttaa
     5641 aaccacccaa aaaagctgct cctcaaaatg atattctgta ttcaaaatat ggcggcgatg
     5701 tagataaaat gatgcaggat atgcgcaggc aagattcgat gaaatacccg tttttataca
     5761 atcttaatga tgaggctaaa aatgacagtt ccactttaac tgatgaacag actaaaattc
     5821 tggattatgc agacgaactt ccggcttttc ctaatggcac agccggactt aacgagtata
     5881 ttctaggcta caagctcaat atccctgatt cactgtcaag cgacaaagct aatgtggaag
     5941 ttaagtttta tgtaaatact acgggtacta ctcaagaccc tgtggtaata aaaacgacta
     6001 acgctaagtt tgatgctttt acaagatggc tcatttatga catgccaact tggactccgg
     6061 cgaaacaaag cggtaaaacg gtaaactgtt atgtcacttt aaaaatcact tacggacagt
     6121 aagcgtagcc tcagagcgta tgtttttttg gtgcttttga ttttttcatc gctttcagtt
     6181 cctcttcaaa atagggtttc aaatggtcaa ttacaatatg tggcacatga caccttcgca
     6241 acgattcttt ttttacaaag acgaatgtcg tttcgcttga agtgagcatt tggttatcct
     6301 gattatatat ttcagaatga aaaaccatat ttttttcagg aaaatcagct atagtcgtct
     6361 tgatgttcat cagctcatca taccgagcag gacggaaata tttgatattc atttccacaa
     6421 ccggcatggc atagccttct tcttctaatt gcttataaga aaaaccgtgt ttgcgcatgg
     6481 cctctgttcg tccaatttca aaaaatttgg cataatgccc atgatagact atccccaagg
     6541 gatcacaatc gtgatagcga acacgcaatt gtatggtttc ttcaaacatt attttacttt
     6601 tttttcattc agtgcttgct gataacgctt ggcattttct tcatgttgtt ccagtgtttc
     6661 agcaaacaca tgatatccgg aaaaatcctc ttttgcacaa aaatacatat acttgctgtc
     6721 attcgcatca agtgtctctt ctatgctctt gcgttcaggc agacaaatgg gaccgggagg
     6781 caagccttta ttgatataag tattataagg tgattgtatt tttaaatcag catagtacac
     6841 acgtcttctt atacccagct tattgagcgc atacataacg gtagggtctg cctgcaaagg
     6901 ccagttttgg tgcaaacgat tgatatatac ttcagcaatt tccgggtatt catcataatg
     6961 atttgattct ttttgaacaa tggaagctaa aatagtggct tctgtgggcg acaaacccaa
     7021 ttcctgcgct ttatcttttc gtcggtcagt ccagaatttg ttatactctt tcagcatgcg
     7081 ttctacaaat tttttggcag aagtattcca gtaaaattca taggtattgg ctaaaaagat
     7141 ggttggaaaa ttatcttcag tcaaattcat ggagtcgagc aaaacaggat tattgaactc
     7201 tttaagcaaa tcacttttac tggcttcaat ttgttttgca atgttttcag ctaattgttc
     7261 tttattctgt atattgttaa atacgatttt aacaggatct tgtgcacctg cacgcagttt
     7321 tctcacaatc gtaaagtttc ccgcattatt ggataataag tatctgccgg gtttcacatg
     7381 cgtatcatat ttcagtgctt tagccactct aataaatgta ttgctgtttt tcaatacttt
     7441 atttgatttc agaatataga gtacatcatc gaaattagct cctgtaggta tcagcaatgt
     7501 ctggtatttc tgtttggaac tgttatttac attatcagca aatgcaacct tataaaaata
     7561 gagaaatata aatagacctg taatgataca aaaaagggca atccaaagta acttttttcg
     7621 catgcagtaa attacagctt tttttgaaag ttagcgtaaa gaaagattct aaaatatttt
     7681 tttgaaaaaa aatcgcattt ttatttgcag aacacaaaaa aatactatat ttgtgtcagg
     7741 gtttttatgt ttctagactt tagggtctgt cgggtttacg caaagtaaac ccgatttttt
     7801 tt
//
LOCUS       DAOQII010000104         7798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5096_length_7798_cov_4.664729, whole genome shotgun sequence.
ACCESSION   DAOQII010000104 DAOQII010000000
VERSION     DAOQII010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5096_length_7798_cov_4.664729"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLF48_06350"
     CDS             <1..335
                     /locus_tag="PLF48_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48983.1"
                     /translation="EIEQNSTHADDSVFPVEAFKKQVTLSCDFFKNLNKMSSQAELEI
                     AAKHELEQLTDLYATSLLFKNINAQTEDWIIPALQHKAWQMQSKETYTSPVHITKENT
                     ERVIAWEY"
     gene            complement(671..1474)
                     /locus_tag="PLF48_06355"
     CDS             complement(671..1474)
                     /locus_tag="PLF48_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF48984.1"
                     /translation="MKIITSPQNPLIKQTLLLQEKSRLRKESGLIVIDGWKETLLAIK
                     NGFQIKTVFYFEQYLSELESDELQQLTITMHTTFVSTSEEVFDKLAFRGRTSKIVSIA
                     KHRTSTFENITENENGLYLVLDGIEKPGNLGAILRIADAAGVNAVFCSDVQTDIFNPN
                     VIRSSVGCVFSQKIICAEKEQILDYFQTKKIQVFTTSLKAAKDYLDVDYTAASAFVFG
                     TEAEGVQSFWEENANQNILITMRGQNDSLNVSNSVAIVIFEALRQRQTR"
     gene            1484..>1968
                     /locus_tag="PLF48_06360"
     CDS             1484..>1968
                     /locus_tag="PLF48_06360"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNF48985.1"
                     /translation="MKNYFVDLNMAKKEIIRCGWCVGKPLYEKYHDEEWGVPVYDDQK
                     QFEFLVLESAQAGLSWWTILQRRENYRKAFADFDYKKVAKFSPEKVELLMQDAGIIRN
                     RKKIEATVNNAKRFMEVQKEFGSFSDYIWSFVGGKPKINKIKSLAEIAATSTESDELA
                     K"
     assembly_gap    1969..2068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2069..3231)
                     /locus_tag="PLF48_06365"
     CDS             complement(<2069..3231)
                     /locus_tag="PLF48_06365"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNF48986.1"
                     /translation="MSGNLLKIFLPLFLYAACSQATAKGIKRYQPAQFSGKIGIEANM
                     HVGRVFRHTTNFRPTINGPSYAIEVSAFKQTDGTKNWQKKIHYPEIGGGFYAVIHHNR
                     DTIGNGFGTYMFWKYPLVRSKVVDFYLKMGAGLGVLTKKYDAIKNPVDNPIGSYVNVF
                     VLIRFGLDWKISRQFKLVTAFTYSHFSDGAVQLPNLGINTPTGTIGLVYYPQNKPIKV
                     NRDTFSRKPVLKNELYAKAGIGMLDVTKFIKEKPWLMQTTSIGYSRYINICNKLSAGL
                     TLEFNFGEPHIYVNTLETEKKKLKKAATELSINIADEILIGRFAMHFELGAYLYHTYR
                     LPLPIYFKLGGAYYLPDMGKKKRSAMFINGNVKAHGATAQLVEGNIGGVWKFN"
     gene            3579..3649
                     /locus_tag="PLF48_06370"
     tRNA            3579..3649
                     /locus_tag="PLF48_06370"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3611..3613,aa:Gln,seq:ttg)
     gene            complement(3703..5583)
                     /gene="htpG"
                     /locus_tag="PLF48_06375"
     CDS             complement(3703..5583)
                     /gene="htpG"
                     /locus_tag="PLF48_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNF48987.1"
                     /translation="METGKINVQTENIFPIIKKFLYSDHEIFLRELVSNAVDATKKLK
                     GLSQLGEATGDLGDLTIQIKADKEKGTLTVSDKGLGMTADEVKKYINQIAFSSAGEFV
                     EKLKNTDGASIIGNFGLGFYSAFMVASKVEIQTKSYKDAPAVKWTCDGSTEFTLDEIE
                     KAERGSDIILYIDDDSKDFLEDYKIKELLTKYCKFLPIPIQFGMKWQSEEDKKEGKPE
                     EPNIINNTQPAWTKSPSSLTDEDYKNFYRELYPMNFEEPLFWIHLNVDYPFNLTGILY
                     FPKLKNKFEVQKDKIQLYCNQVFVTDHVGEIVPEYLTLLHGVIDSPDIPLNVSRSYLQ
                     SDANVKKINQHISKKVSDKLQEMFNNDRVQFEEKWNEISLFVKYGMLSDEKFYERSIG
                     YALFQTTDGAYYTLDELKNKIKDNQTDKDGNLVILYTSDTEEQHSYIQQVKEYGYEVV
                     KLETVIDNHFIQQLEMKNTSGDGNDKLQFKRVDADIVANLIPKDEAQESVMTQEQQDT
                     LKSLYENVVEKSKADIELKALSPKDAPVLITRNEFMRRMAEMQKMGGGSPFMFGGMDE
                     KLNLVVNTNNPLNSKLLAESAEKQTEYIKQLYDLALLSQNMLKGEALTNFVNRSFSLF
                     VE"
     gene            complement(5945..6796)
                     /locus_tag="PLF48_06380"
     CDS             complement(5945..6796)
                     /locus_tag="PLF48_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48988.1"
                     /translation="MKIFSTLLILLLFFVTQISIAQKTKSKSTSKTTPANNKSKEVTA
                     TKKETLPSKKNNETKNSVQSTKGCSSCPGFEGIVLSTNSNGNFSFDSIAKSYNTNVNT
                     ELVGGIWKYTNTILINKKGELVKILRAYYNDLDQSLYNLNNKPASADPQPGDQDIMND
                     PAQKMKKENERLGRDKFNPSDNLLVFDPNVDYLFLDKPGNTEDSYVIRKKRPDNTIIQ
                     LTNADIRFKFENNAKTNQVLFTLQEHDEPISKEFVVLKLNNDSFIIADYSFKEIHFFD
                     RIGIHKE"
     gene            7410..>7798
                     /locus_tag="PLF48_06385"
     CDS             7410..>7798
                     /locus_tag="PLF48_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48989.1"
                     /translation="MGNKLMSLQARHLFFKRAMALTTITQQAWNNSTNSGSENLTDMS
                     ASTFKSSKMKNTKLTSLVFALFAMVLMFTSKNAMASPGNKIFIGLPSPGAVPLAGEMH
                     FCEPDTFRFQITNLTSDPLTNMVAHIV"
BASE COUNT         2362 a         1361 c         1352 g         2623 t
ORIGIN      
        1 atgagattga acaaaatagt acacatgcgg atgattctgt ttttcctgtt gaagcgttca
       61 aaaaacaagt cacattatct tgcgatttct ttaaaaactt aaacaaaatg agcagtcaag
      121 ctgaattgga aattgctgct aaacatgaat tagagcagct taccgatttg tatgctacat
      181 cattattatt taaaaatatt aatgcccaaa cagaagattg gataatacct gcattacaac
      241 ataaagcatg gcagatgcaa agtaaagaaa cttatacatc gccggtacac attaccaaag
      301 agaacacaga acgtgtaata gcctgggaat attaatgtct cgcataacaa aatttatttc
      361 attattctac taacttctta gtgcattttg atttataatt attaccacat agtggtatgg
      421 atttttaggt ttaagaacac ttacagcatc ataaattggg gtatgaatag caaaatacag
      481 gtatcgacaa aggcgatact atgtttttcc tgactatcta aactgttgtg caccaatagc
      541 tatttgtttt taaaaatgct atgtttctat gtggtttaaa cagtactacg ggtactatcg
      601 ttgttttttt tgtgtttaat aaataatttt tttaagttta atggaagaat ttaaactact
      661 caccatggtt ttatcttgtt tgtctttgtc ttagagcttc aaaaattaca atggcaacgg
      721 agttggagac attcaaagaa tcgttttgcc cccgcatggt aatcaatata ttttgatttg
      781 cattttcttc ccaaaaggat tgtacacctt ccgcttccgt accaaaaaca aatgcacttg
      841 cagctgtata atcaacatcc agataatctt ttgcagcttt taaagaggta gtaaaaactt
      901 ggatcttttt ggtttgaaaa tagtccaaaa tttgctcttt ctcagcacag attatttttt
      961 gagaaaacac acaacctact gacgaacgaa tgacattcgg attgaaaata tccgtctgta
     1021 catcagaaca aaatactgca ttaaccccgg ctgcatctgc aatgcgcaaa attgcaccga
     1081 gattgccagg tttttcaatg ccatccaaaa caaggtatag accattttca ttttctgtta
     1141 tattttcaaa agtagatgtc ctgtgttttg caatacttac tattttgctg gttcggccgc
     1201 gaaatgccag tttatcaaaa acttcttcag aagtggaaac aaaagtagtg tgcatagtta
     1261 ttgtcagttg ttgcaattca tccgactcaa gttcacttaa atattgctca aaataaaaga
     1321 ctgtttttat ttgaaatcca ttctttatgg ccagcaaagt ttctttccac ccgtcaataa
     1381 caattaatcc gctctcttta cgcaggcggg atttttcctg caataataag gtctgtttta
     1441 tcaacggatt ttgtggtgat gtaattattt tcacgttaca aaaatgaaga attattttgt
     1501 agatttaaac atggcaaaaa aagaaatcat tcgttgcggc tggtgtgtgg gcaaaccgct
     1561 ttatgaaaaa tatcatgatg aagaatgggg tgttcctgtt tatgacgatc aaaaacaatt
     1621 cgagttttta gtgttagaaa gtgctcaagc tggtttaagc tggtggacaa tcttgcagag
     1681 acgggaaaat tacaggaagg catttgctga ttttgactat aaaaaagtag ccaaattttc
     1741 accggaaaaa gtggaactcc ttatgcagga tgcaggcatc atccgcaata gaaaaaaaat
     1801 agaagctact gtcaacaatg caaaaagatt tatggaagta caaaaagagt ttggttcttt
     1861 ttccgattat atatggtcat ttgttggtgg aaagccgaaa ataaacaaga taaaaagcct
     1921 tgctgagatt gcagcgacca gcacagaatc tgatgaactg gcaaaagann nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt attaaatttc caaacaccgc cgatattgcc
     2101 ctctaccagt tgcgctgtag caccatgtgc ctttacgttg ccattgataa acatggctga
     2161 gcgttttttc ttacccatat ccggcaaata gtaagcacca cccagtttga aataaatggg
     2221 caaaggtagg cgataggtat gatataaata agctcccaat tcgaaatgca tggcaaacct
     2281 gccaatcaat atttcatctg cgatattaat actcaattca gtggcggctt tttttaattt
     2341 cttcttctcg gtttctaatg tattcacata aatatgcggt tcgccgaaat taaattcgag
     2401 tgtcagtcct gcagatagtt tattgcaaat attgatatat cgcgaatagc cgatactggt
     2461 tgtttgcatg agccagggtt tctctttgat gaatttggtt acatccaaca tgccgatacc
     2521 ggctttggca taaagttcat tctttaatac cggctttctt gaaaatgtat cccggtttac
     2581 tttaatcggt ttgttttgcg gataataaac caaaccaata gtgccggttg gggtattgat
     2641 gcctaagttg ggcaattgca ccgcaccgtc tgaaaagtgc gagtaagtaa aggccgtgac
     2701 caacttaaat tgccttgaaa ttttccagtc taaaccgaaa cgaattaaga caaaaacatt
     2761 aacataagaa ccgataggat tatcaaccgg attcttaatg gcatcatatt ttttggtaag
     2821 cacacccaaa cccgcaccca tcttgagata aaaatccact actttacttc gtactaaagg
     2881 atatttccaa aacatatacg taccaaaacc gtttccaata gtatctcgat tgtgatgtat
     2941 taccgcataa aagccaccgc ctatttcagg ataatgtatt tttttttgcc agtttttagt
     3001 gccgtctgtt tgtttaaatg cactgacttc aatggcatag ctgggaccgt ttatagttgg
     3061 tctgaaatta gtagtatgcc gaaatactct gcccacatgc atgttggctt ctatgcctat
     3121 ttttccggaa aactgtgccg gttggtagcg ttttatgcct ttggctgtag cctggctaca
     3181 ggctgcataa aggaacaggg gtaagaagat tttcaggaga tttccggaca tcaggcgtac
     3241 ttttcagttg tttctatgca aaagtatcat tttttctttg tgatgtattc atatgaaatt
     3301 tactggtatc tgagttcggg ttaagaaaaa taagctgata aaatagttga tttcggtata
     3361 atggacattt gtggaggttt tttatagacg ttttgtccat gtggacattt gtggaggtgg
     3421 ttcttcataa aaaatcaact atttttttaa ttgaaacgat atcccgaact caggttggta
     3481 ggatgagagt tatcttgttt cgtaaacatt ttttaaaaga ccggagaaaa aaacgttttg
     3541 aatattttga aaaatatgta actttgcacc tcacatcaag gctgatggtg taacggtagc
     3601 acagaggttt ttggtgcctt tagttaaggt tcgaatcctt atcggcctac aaaaaaagtc
     3661 ccgcattagt gggacttttt tgttttttgt tgaaatcgta atttattcca caaaaagaga
     3721 aaaacttctg ttcacaaaat tggtcaatgc ttcgcctttg agcatatttt gagagagcaa
     3781 tgccagatcg taaagttgct tgatatattc tgtctgtttc tcagcagact cagcaagcag
     3841 tttactgttc aatggattat tggtattgac tactaaattt agtttttcgt ccataccgcc
     3901 aaacataaat ggcgatccgc cacccatctt ttgcatttca gccatacggc gcataaattc
     3961 gtttcgtgta atgagtacag gtgcatcttt aggagaaaga gctttcagtt cgatatctgc
     4021 tttggatttt tcaactacat tttcgtacaa agattttaat gtatcttgtt gttcttgtgt
     4081 catgacagat tcctgtgcct catcttttgg aatcaggtta gccactatgt cggcatccac
     4141 acgtttaaac tgcaacttgt catttccatc acctgatgtg tttttcattt ctaattgctg
     4201 gataaaatga ttgtcgatga ccgtttctaa ttttaccact tcgtagccgt attcttttac
     4261 ttgctgtata tagctgtgtt gttcttccgt atccgaagta tagagaataa cgagatttcc
     4321 gtctttatca gtttggttgt cttttatctt atttttcagt tcatccagcg tataataagc
     4381 accgtcagta gtctgaaaca acgcgtaacc gatgctgcgc tcataaaatt tctcatcgct
     4441 cagcataccg tattttacaa acaaactgat ttcattccat ttttcttcaa attgcacacg
     4501 gtcgttatta aacatctctt gcaatttatc cgaaactttt ttggagatat gctgattgat
     4561 tttttttaca ttcgcatcgc tttgcagata agaacgagat acatttagcg gaatatccgg
     4621 cgagtcgatc acaccatgca agagcgttaa atattcaggt acaatttcgc ctacatgatc
     4681 ggttacaaaa acctgattgc aatataactg tattttgtct ttttgtactt caaatttgtt
     4741 tttcaattta gggaaataca aaatgccggt tagattaaac ggatagtcca cattgagatg
     4801 aatccagaac aaaggctctt caaaattcat cgggtacaat tcccggtaaa aatttttata
     4861 atcttcatcc gttaagctcg aaggcgattt tgtccatgca ggctgtgtgt tgttgatgat
     4921 attcggctct tcgggtttgc cttctttttt gtcttcttcg ctttgccatt tcatcccaaa
     4981 ttgaataggg atgggcaaaa acttacaata ttttgtaagc agttctttta ttttataatc
     5041 ttccagaaaa tctttagaat catcgtcgat atataagatg atatccgaac ctcgttctgc
     5101 tttttcaatt tcatccaaag taaattcggt agagccatca caagtccatt ttaccgccgg
     5161 ggcatcttta tacgatttcg tttgaatctc cactttagaa gccaccataa aggcagaata
     5221 aaaacccagg ccgaaattac caatgataga agcaccatca gtatttttta gtttttctac
     5281 aaattcgccg gcacttgaga aagcgatttg gttgatgtat ttttttactt catctgctgt
     5341 catacccaac cctttatctg agacggtgag tgtacctttc tctttatctg cttttatctg
     5401 aatggttaaa tcgccaaggt cgccggtagc ttcaccgagc tgagaaagac ctttcagttt
     5461 tttggtagca tctacggcat tggagaccag ctcgcgcaga aatatttcat ggtcagagta
     5521 aaggaatttt ttgataatgg ggaagatgtt ttccgtttgt acattaattt ttcctgtttc
     5581 catattgtca gttatttata aaattgatat gatgattaac taaaccgcaa aaaatcaaga
     5641 attataccat actaaaatgc tgacaaagtg gcgtaaaaca ggatttttaa tggttaatga
     5701 tgaattgaaa atgcagaatg gagaatcaat gtacccgtgt cataagaagc ttccatcttt
     5761 tctcgtgctg tcagatggaa acatctgacg gataattttc ttaccgattc aatttcgttc
     5821 cgtcaggtcg gaagaccaga cggcaaagtg aaatagttta ttcagtgcta tttgtcagtc
     5881 aaaaaaaatg cacaaaagat atttgataaa acataaaaac gcaagccgga taaatataaa
     5941 aagattattc tttgtgaata cctattctgt caaagaaatg aatttcttta aaagaataat
     6001 ctgctataat aaaagaatca ttatttagtt taagcacgac aaattctttt gagattggtt
     6061 cgtcatgttc ttgtaatgta aacaatactt gatttgtttt tgcattattc tcaaacttaa
     6121 aacgaatatc tgcatttgtt aattggataa tagtattgtc aggtcttttt ttacgaatta
     6181 cataactatc ttcagtattg ccaggcttat ctaaaaataa ataatccaca ttgggatcaa
     6241 acactaagag gttatctgaa ggattgaatt tatctcttcc taatcgttcg ttttcttttt
     6301 tcatcttttg agccggatca ttcatgatgt cttgatcacc gggttgtgga tcagcggaag
     6361 caggcttgtt atttaagtta tataatgatt ggtctaaatc attatagtag gctcttagta
     6421 ttttgactag ctcacctttt ttgtttatta aaattgtgtt tgtgtatttc caaattcctc
     6481 cgacaagctc cgtatttaca ttagtgttgt agctttttgc aattgaatca aaactaaaat
     6541 ttccattact atttgtactc aatacaattc cttcaaaccc aggacatgat gaacaacctt
     6601 tggtactttg aacagaattc tttgtttcat tatttttttt agaaggtaat gtttctttct
     6661 tcgtagctgt tacttcttta cttttattat tggcaggtgt cgttttcgaa gttgattttg
     6721 attttgtctt ctgagcgata ctaatttgag taacaaaaaa cagaagcaat attaataagg
     6781 tactaaaaat tttcatgatt aattttttgc gaaagtaata aaaaaacata attacgatac
     6841 agcaaatatt ttgccaagaa aatactttaa ttgtcgaggt ccatgttatg tctatataat
     6901 tacgttttca atttaaccct taagactata atgattcctt ttctgtgaag tcacttggaa
     6961 acaaatgact aaattattca atgacctcac taacattcgt caagacacta caaaaaatta
     7021 ataaaagttc ttcagataag ccttatttca tgttgtgatt caaatgaggt ttcatttata
     7081 tatcggcatt gtagttccta ttgtgtttaa taaatcctta aatacaccaa atattttgat
     7141 aaacatcaaa acgcaagtac ttcattacca gtatgcatta gaactatttt taataaaatc
     7201 tttacatttt ttaacctaac tattgcaaat gtggtataag tacactattt ttgtcattca
     7261 aatgtggaaa actctatact tgtatagaca tttttcacaa atgggggact aactaactaa
     7321 ctaactaact ttgtttaaca aagaaaatac atcagaacgc atgttggctg tttttaacag
     7381 ctgcatgcgt tttttttaaa cggaataaaa tgggaaataa attgatgagc ttacaggcaa
     7441 gacacttatt cttcaaaaga gcaatggcat taacaaccat tactcaacaa gcatggaata
     7501 attcaactaa ttccggttct gaaaatctga cggatatgtc agcttcaact tttaaaagta
     7561 gtaaaatgaa aaatactaaa ctcacttcct tagtgtttgc gttattcgca atggtgttga
     7621 tgttcacctc taaaaatgct atggcttccc cgggcaacaa aatctttatc ggattgcctt
     7681 ctcccggtgc tgtgccatta gcaggtgaaa tgcatttttg tgaaccggat actttccgtt
     7741 ttcaaatcac taatcttacc agtgaccctt taacaaatat ggttgcacat attgtttt
//
LOCUS       DAOQII010000105         7739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5145_length_7739_cov_5.347345, whole genome shotgun sequence.
ACCESSION   DAOQII010000105 DAOQII010000000
VERSION     DAOQII010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5145_length_7739_cov_5.347345"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLF48_06390"
     CDS             <1..278
                     /locus_tag="PLF48_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48990.1"
                     /translation="LDNLLALKRKQQQLQTTILNLYFILLSSGILLYMIEPSLRMTVF
                     WRIMSYALSIIWFLFCWFYLRPRQTKKQQSKLDELIKKYELLQSQLS"
     gene            complement(350..1384)
                     /locus_tag="PLF48_06395"
     CDS             complement(350..1384)
                     /locus_tag="PLF48_06395"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNF48991.1"
                     /translation="MKKIVLVIAVLFAIAGNTFAQVEKQIQGEAMNAAEKKKMDDTLN
                     GWKFGGMGGLTFNQAGFKNWAPGGVNSFSMLFNLRLSADYKKGKHLWQNWAAAEYGLQ
                     FIKGDYHKVRKNADRWEIFSKYGYKVYKPLYVAAYADLRSQFSKSYTYADTTKTLTSR
                     FASPLTFEAAIGLDYVPNAHFSLFASPLAAKIVYVGDDAIAMAGTFGNAWPKKHRDEF
                     GAVLIATYKQDFWKQNISILSVFKVYKNYLRSDYEKSATINSFDNAGKKHYRQNFDID
                     WQTTLGLKVNNWLSASVFMQLVYDNDVIFAKDANNITSTKAQFRDVIGVGLTYQTNYF
                     KAKGEKAKKL"
     gene            complement(1708..3846)
                     /locus_tag="PLF48_06400"
     CDS             complement(1708..3846)
                     /locus_tag="PLF48_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF48992.1"
                     /translation="MKLITPKITAFLCIFTLYKLGFCQLTLENIFLYKRYEPKLIDEI
                     HFLNQSPQFAILETTGKQQNISIYNTQNNLTKKVPLIFPGSWSNFSFSNTDKYILLES
                     DAHYIYRRAYEANYFILNEQHELQNIYANKVMFPQFSTDDSKIAFVSKNNLFYKDLKA
                     DKIIQITKDGKQNYIINGKSDWVYEEELQLTQAYKWSPNGTHIAYLKFDESKVKEYSF
                     PVYGSENYPQNFIYKYPKAGEENSKVTLWLYNLKKKKNTAIRLPTDYEYIARLYWNFE
                     GDELLVLTLNRKQNELQLLSYNLSSKSWHTCNKEQSDTYIDLQDNVLFLKDNSFFITS
                     EKEGYNQLYHYTKNGELIKKITTDTKNIDALYSVDENSKKVYYQSVEKEGLETVIMSC
                     RYDNLNQELLSKQRGDNRAVFSKDGSFYFQTYSSDSIPPQISLHNLSNDSIVLLIDNK
                     ALKDSLKDIPRKTYTTLPVNGNNLNTYLIKPTNFDSTKKYPLLMYVYGGPGNIEVSNK
                     WQSSRLFWLNMLAEQGYFIACIDNRGSGGKSADFKKTTYLHLGKYETQDQIAAAQYLG
                     KLPYIDKSRIGIFGWSYGGFMAANCLFEGNDVFKLAIAGAPVSNWRFYANIYTERYMQ
                     KPEENKQGYQEYCPTVLAEKLKGKFLLVHGTADDNVHFQNSIQLIHALQQAHKPFDLY
                     IYPDKAHSISGSATRFDLYSRLTQYIISNL"
     gene            3892..4581
                     /locus_tag="PLF48_06405"
     CDS             3892..4581
                     /locus_tag="PLF48_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF48993.1"
                     /translation="MKSAILFLSVLFCTTVSLKAQEEIYQPTTIRDTVITNNNGSAPT
                     ENTKKDQQTDKKQAQDEDARTKQEKKLDRLRHLRLGGNFGLQFGSYTYINISPTVGYL
                     FLKDRLEAGAGPIFIYEHYRFSNTVSYNFFVTGMDVYAKGYIFRGLYAMAQFDLINKP
                     SYVYYNKRVTVPHFLIGAGYSVPMGKVGYFNLQAMFSVINNSETIYRGTFGNFPLLFT
                     MGFSFGAAKQD"
     gene            complement(4601..5716)
                     /locus_tag="PLF48_06410"
     CDS             complement(4601..5716)
                     /locus_tag="PLF48_06410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNF48994.1"
                     /translation="MHSDLTLIFSALFTAFVLTYFFIPSIIKVAKIKHLYDVPDDHRK
                     SHSNVIPTLGGIGIFGGFMIAFCLFANFSLANREIKYVLAALCFTFMLGAKDDIVDLV
                     ANKKFIGQIFSAAIIVVLGNIRITSMYGLFGITFLDTYSSIFLSILTIIFIVNAFNLI
                     DGINLLAGSISIVISVAFGAWFYVYGFTDYAVLAAAMSGAVLAFLKYNFTPARIFMGD
                     SGSMSIGLLAAILAIQFIEMNELILNKGISIGQPIVASPAMAIAVLIIPIYDALRVFA
                     LRILARKSPFSADRNHIHHKLLDIGLSHLQATVILIAVNILFIIAVYLLQDWGNLVLI
                     ILEILMATLLTLLLFLIKDKKKAEVKLPKRKVDLSLH"
     gene            complement(5764..6966)
                     /locus_tag="PLF48_06415"
     CDS             complement(5764..6966)
                     /locus_tag="PLF48_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF48995.1"
                     /translation="MVINRIPKFLEKICGIKINVLKSLANIQHIGISANTAFNLYNFR
                     LGLIKALQNAGYKVFAIAPYDEYADKLIAEDIPFIEVKQLARKGTNPFQDFLLMNEYR
                     KIFKKNKIDIVLQYTIKPNIYGTLAAVFTNTKTICTVTGLGYTFLNDSLSNKIAHRLY
                     KLSFGKANIVLFQNADDLQLFLEKGLVQKDKAGVVNGSGIDTDYFYPEFCKDKINNEQ
                     QKQTAFLMVARLLKDKGVYEYITAATKILSENKNAVFHLLGALDDGNPAAIKKQELED
                     WINSGIIIYHPHTHDIRSYYCDADCVVLPSYREGMPRVLLEAMAMGKPCITTDAPGCK
                     DAVVEDNTGFICSTANSASLAFCMRNFINLNIEYKNQLGINARKRAINEYSITHIVKF
                     YFDLIKKV"
     gene            complement(6918..>7739)
                     /locus_tag="PLF48_06420"
     CDS             complement(6918..>7739)
                     /locus_tag="PLF48_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNF48996.1"
                     /translation="VTVALSGDAGDELFAGYNKHYGEWQIRNGGWKADAIHLLKPLWE
                     KLPKSRSGKMANKIRQLERFANGMDLSPAERWWQWCTFFGLDNVNKLLNGKTDMVLPQ
                     IISTRQQYMSLVQHDGTINDDLLADVSMVLPYDMLVKVDRMSMRNSLEVRVPFLDYRI
                     VNFAFSLPVSSKINAMYKKRIVQDAFRNYLPKELYNRPKKGFEVPLLQWFRTDLKSKI
                     LDDYLSDEYIRAQGVFNPEVISEIKQQLFSSNPGDVHARIWSLIVFQNFWRKYVE"
BASE COUNT         2380 a         1467 c         1311 g         2581 t
ORIGIN      
        1 atttagacaa tttgctggct ttgaaaagga aacaacaaca gctgcaaacg acaattttaa
       61 atctgtattt tatcctgctt tcttccggta tcttattata tatgattgaa ccatccttgc
      121 gaatgacagt tttttggagg ataatgtcgt acgcgttgtc catcatttgg ttcttgtttt
      181 gctggtttta tttaagacca agacaaacaa aaaaacagca aagtaaatta gatgaactta
      241 tcaaaaaata tgagttacta caatcacaac ttagctaaac aggaacatta taaaaaataa
      301 aaaaagcccg catttttttg cgggcttttt tcatttggcg gttaatggtt tatagttttt
      361 ttgctttttc gccttttgct ttgaaatagt tggtttggta agttaaacct actccaatta
      421 catccctgaa ttgggcttta gtgctggtaa tattattggc atcttttgca aagatgacat
      481 cattatcata taccagttgc ataaatacgc tggcagacaa ccaattgttc acttttaaac
      541 cgagtgtagt ttgccagtcg atgtcaaagt tttggcgata gtgtttttta cctgcattat
      601 caaaactgtt gattgttgca cttttctcgt agtctgatct caaataattt ttatacacct
      661 tgaatactga caatatactg atattttgtt tccagaaatc ttgtttatat gtggctatta
      721 atactgcacc aaactcatct ctgtgttttt ttggccaggc attaccgaat gttcctgcca
      781 ttgcaatagc atcatctcca acatacacga tcttcgctgc caatggtgat gcaaataatg
      841 agaaatgtgc attgggaaca tagtccaaac caatggctgc ttcaaacgtt aaaggagaag
      901 caaatctgga tgtcagtgtt ttggttgtat cggcataagt atacgatttg ctgaattgtg
      961 aacgcaaatc tgcgtaggct gcaacataaa gtggtttata aaccttatat ccgtatttag
     1021 agaaaatttc ccaacggtca gcatttttgc gtactttatg ataatcgcct tttataaatt
     1081 gcaagccata ttctgcagcc gcccagtttt gccataagtg ttttcctttt ttgtaatcag
     1141 cacttagtct cagattaaat agcatagaaa atgaattaac tccacccggt gcccagtttt
     1201 taaaaccagc ctgattaaat gtcaaaccgc ccataccacc aaacttccat ccgttaaggg
     1261 tatcatccat ttttttcttt tctgctgcat tcatggcttc accctgaatc tgtttttcta
     1321 cttgagcaaa ggtattacct gcgatggcaa ataacacagc gattactaaa acgatctttt
     1381 tcataaaaat tttttgtttt tttagcgtta attaaaagat tagaatattt attaatatta
     1441 gttagctgaa atgacgtgca aagttaagtt gtcgttgcat ctattctgta aactaaatgt
     1501 tacggaatag ttgtcatggc aactaattaa aaaatcaccg tttatgtgta aagaaaacct
     1561 gtgttcggga taagagtttc tactaaaaac agttgatttc ttatcaataa ccagctcctt
     1621 ttaattcttc ctccttgtta agttggagga aaaaatgaga aatcaactat tttttttcac
     1681 cttttttttc ttaacccgaa ctcaggctta tagattgcta ataatatatt gagtgagccg
     1741 tgaatacaag tcaaaccttg tagctgatcc cgaaatacta tgtgccttat ccggataaat
     1801 atacaaatcg aatggcttgt gagcttgctg taaggcatga attaattgaa tggagttttg
     1861 aaaatgcaca ttgtcatctg ctgtgccatg taccagcaaa aattttcctt taagtttctc
     1921 agctaaaacg gtcgggcaat attcctgata gccctgcttg ttttcttcgg gtttttgcat
     1981 atatcgttcg gtatagatat tagcataaaa acgccagttg ctgacaggag ctccggcaat
     2041 agccagttta aaaacatcat tgccttcaaa caaacaattg gcggccataa aaccgccata
     2101 actccagcca aaaatcccga ttcggctttt gtctatatag ggcaatttac ccaaatattg
     2161 tgcagccgca atttggtctt gagtttcata ttttcctaga tgtaaatatg ttgttttttt
     2221 aaaatctgca cttttcccac cggagcctct gttatctata caggcaataa aataaccttg
     2281 ctcagccaac atattcagcc aaaacaaacg gctgctttgc catttattac tgacttcaat
     2341 attaccaggt cctccataaa catacatcaa taagggatat tttttagtcg agtcgaagtt
     2401 ggtcggttta atcaaatagg tattcaaatt attgccatta acaggcaaag tcgtataggt
     2461 ttttctggga atatctttaa gtgagtcttt taatgcctta ttgtctatca acaaaacaat
     2521 actgtcattc gataagttat gtaaagaaat ctgtggggga atactgtcac tcgaataggt
     2581 ttggaaatag aaactaccat ctttggagaa aacggctctg ttatcacccc gttgcttact
     2641 cagtagctct tggtttaagt tatcgtaccg gcaactcatt attacggttt ccagtccctc
     2701 cttttctacc gattggtaat atactttctt tgaattttcg tccacgctgt agagcgcatc
     2761 tatatttttt gtatctgtag ttattttttt aattaattcg ccgttttttg tataatgata
     2821 gagttgatta tagccttctt tctcagatgt aataaagaaa gaattatctt tcagaaaaag
     2881 cacattatcc tgcagatcaa tatacgtatc cgattgttct ttattgcaag tatgccacga
     2941 ttttgaggac aaattataag agagtaattg aagctcattt tgtttccgat taagtgtcaa
     3001 aacaaggagt tcatctcctt caaaattcca atacaacctt gcgatatact cataatctgt
     3061 cggtaacctt atagccgtgt tttttttctt tttgaggtta taaagccaga gtgtaacttt
     3121 tgaattttcc tcgcctgctt tgggatattt ataaataaaa ttttgcgggt aattctcact
     3181 gccatacacc ggaaaagaat attcttttac cttactttca tcgaatttca aataagcaat
     3241 atgggtacca ttcggactcc atttataagc ttgtgtcaat tgaagttctt cttcatatac
     3301 ccaatcgctt tttccgttga tgatgtaatt ttgctttccg tctttagtaa tttgtattat
     3361 tttgtcagct ttaaggtctt tgtaaaaaag attatttttc gacacaaatg caatcttaga
     3421 atcgtcagta gaaaactgag gaaacattac tttattggca taaatatttt gcagctcatg
     3481 ctgctcgttc aaaataaaat aattggcttc atacgctctc cgataaatgt aatgagcatc
     3541 actctcaagc aaaatatact tatcagtatt tgaaaatgaa aaattactcc agctacccgg
     3601 aaagattaaa ggaacctttt ttgtcaggtt attttgggta ttataaatgg aaatattttg
     3661 ttgttttccc gtagtttcca gtattgcaaa ttgcggactt tggtttaaaa aatgaatttc
     3721 atcgattaat ttgggctcat atctcttata taaaaaaatg ttttcaagtg ttaattgaca
     3781 aaatcctaat ttataaaggg tgaaaatgca taaaaatgcc gttatttttg gagtaattaa
     3841 tttcatgcac agtttttgca ctaaattaag gcattcaata aaaattaaaa aatgaaatct
     3901 gcaatcttgt ttttatcggt tctgttctgc actaccgtat ctttaaaagc acaggaagaa
     3961 atttatcagc caaccactat ccgtgatacg gtaattacaa acaataacgg ctctgctcct
     4021 actgaaaaca caaagaaaga ccagcagacg gataaaaagc aagcacaaga tgaagatgcc
     4081 cgtactaaac aagaaaaaaa actagacaga ttgcgacatc ttcggcttgg cggaaatttt
     4141 ggtttacagt ttggcagcta tacctatatc aatatctcac cgacggtagg gtatttattt
     4201 ttgaaagacc gattagaagc cggtgccggt ccgatattta tttacgagca ttatcgcttc
     4261 agcaatacgg tttcatacaa tttttttgta accggaatgg atgtatatgc caaaggatat
     4321 attttcaggg gattgtatgc catggcgcaa tttgatttaa ttaataaacc atcatacgtg
     4381 tattacaata agagagttac cgtgccgcat tttctgatag gtgccggtta ttccgtaccg
     4441 atggggaaag tgggctactt caacttgcaa gctatgttta gcgtaatcaa taacagtgaa
     4501 acaatttaca ggggcacatt cggaaatttc ccattgttgt ttacgatggg ttttagtttt
     4561 ggagcggcaa aacaagatta agaaaatagt cttttatacc ttaatgaagg gataaatcca
     4621 cttttctttt aggtagcttc acttctgctt tttttttatc ttttattaaa aacaacaata
     4681 aagtaagtag tgttgccata agaatttcga gtatgataag tacgagattg ccccaatctt
     4741 gaagcaaata aacagcaatg ataaataaga tattaacggc aatcaagatg acagttgctt
     4801 gtaaatgact caatccgatg tctaataact tatgatgaat atgatttctg tcggcagaaa
     4861 atggagattt tcttgccaaa atacgcaaag caaataccct taaagcatca tatataggaa
     4921 ttattaacac ggcaattgcc atagcaggag atgccactat cggctgcccg atactgatgc
     4981 ctttattcag tataagttca ttcatttcaa taaactgtat ggccagtata gctgccaaca
     5041 gcccgataga catactcccc gaatcgccca taaatatccg tgccggagta aagttgtatt
     5101 ttaaaaatgc cagcactgct ccgctcatcg ctgcggctaa tacagcataa tcggtaaatc
     5161 cgtatacata gaaccacgca ccaaaagcaa cagatataac tatactgata ctgcctgcca
     5221 acaagttgat gccgtcaatc aaattaaaag cattaacgat aaagatgata gtgagtatag
     5281 ataaaaaaat actgctatac gtatcaagaa atgtaatacc aaacagaccg tacatactgg
     5341 taatgcggat attacccaaa accacaataa tcgctgcaga aaatatttgc ccgataaatt
     5401 tcttatttgc caccaaatca acaatatcat ctttagcgcc caacataaat gtgaaacaaa
     5461 gggctgccaa tacatatttt atttctctgt tggccaacga aaaattcgca aataagcaaa
     5521 aggcaatcat aaacccgcca aaaataccaa tgcctcctaa tgtaggaatc acatttgaat
     5581 gcgattttcg atggtcatcc ggtacatcat acaaatgttt tattttcgcc actttgatga
     5641 ttgacgggat aaaaaaatat gtaagcacaa atgccgtaaa aagtgctgaa aatatgagtg
     5701 ttaagtcgct gtgcattcca acttataaag ttagtaacat taattttaaa tcagacaaaa
     5761 aaattaaact tttttaatta aatcaaagta gaacttaaca atatgagtta ttgaatattc
     5821 attaatcgct ctttttcttg catttatacc tagttgattt ttatattcta tattgagatt
     5881 aatgaaattt ctcatacaaa atgccaatga agccgaattt gccgtactac agataaatcc
     5941 tgtattatct tctacaactg catctttgca gccgggtgca tcagttgtaa tgcatggctt
     6001 gcccatggcc attgcctcaa gtaataccct gggcattcct tctctataag atggcaacac
     6061 cacgcaatcg gcatcacaat aataagaacg gatatcatgt gtgtgtggat gataaataat
     6121 aatgccactg tttatccaat cttccagttc ctgttttttt atggccgccg gatttccatc
     6181 atccaaagcc ccgagaagat gaaatactgc atttttattt tcactcaata ttttcgtggc
     6241 ggctgttata tattcatata cccctttgtc tttcaataaa cgtgctacca tcagaaaggc
     6301 agtttgtttt tgttgttcgt tgtttatttt atctttacaa aattccggat aaaagtaatc
     6361 cgtgtcgatg ccagatccat ttactacgcc ggctttatct ttttgcacta atcctttttc
     6421 taaaaagagc tgcaaatcat ctgcgttttg aaataaaaca atatttgctt ttccaaatga
     6481 cagtttataa aggcgatgtg caattttatt agacagacta tcattcaaaa aggtgtatcc
     6541 taagccggtt accgtgcata tcgttttcgt gtttgtaaat actgctgcca atgtaccata
     6601 gatattgggc ttaatcgtgt attgcaacac aatgtctatc ttgttttttt tgaatatttt
     6661 gcggtattca ttcatcagca aaaaatcctg aaatggattt gtgccttttc ttgccagttg
     6721 ttttacttca ataaatggaa tatcctctgc aattaatttg tccgcatatt catcataagg
     6781 agcgatggca aaaactttgt atcccgcatt ttgcaatgcc tttatcaatc ctaatctgaa
     6841 attatagaga ttaaacgccg tattagcact aatgccgata tgttggatat tagccaatga
     6901 ttttagtacg ttgattttta ttccacatat tttctccaga aattttggaa tacgattaat
     6961 gaccatattc ttgcatgtac atcgccggga ttggatgaaa aaagctgttg ctttatttcg
     7021 cttattactt ccggattaaa aacaccttgt gcccgaatgt attcatcact cagatagtcg
     7081 tccagtattt tagattttaa atctgtcctg aaccattgca ataaaggtac ttcaaatcct
     7141 ttcttcggcc ggttgtataa ttcttttggc aaataatttc ggaatgcatc ctgcacaatg
     7201 cgcttcttat acattgcatt tattttggat gaaaccggta aagaaaatgc aaaattgaca
     7261 atgcggtaat caaggaaagg cacgcgcact tctaggctgt tgcgcatact cattctgtct
     7321 accttcacca gcatatcata cggcaatacc atgctgacat cagccagcaa atcatcattt
     7381 atcgtaccgt cgtgttgcac aagcgacata tactgctgtc tggtactgat aatttggggt
     7441 aataccatgt ctgttttgcc gttcaatagt ttatttacat tgtccaaacc gaaaaacgtg
     7501 caccattgcc accagcgttc agccggtgat aaatccatac cgtttgcaaa tcgctccagt
     7561 tgtctgattt tgttggccat ctttccactt ctcgatttgg gtagtttctc ccataaaggt
     7621 ttcagcaaat gaatagcatc tgctttccag ccgccgtttc ttatttgcca ctctccgtaa
     7681 tgtttgttat atccagcaaa aagctcatct ccggcatcgc cactaagtgc aacggttac
//
LOCUS       DAOQII010000106         7649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5223_length_7649_cov_5.276929, whole genome shotgun sequence.
ACCESSION   DAOQII010000106 DAOQII010000000
VERSION     DAOQII010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_7649_cov_5.276929"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLF48_06425"
     CDS             <1..644
                     /locus_tag="PLF48_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNF48997.1"
                     /translation="DIIHQKNSIFFCDATQAMGKIPVDVDKDGIDMLCISGHKIYAPK
                     GVGCLYTRRKNPRVSLEPLLHGGGHEQGKRSGTLNIPGIVALGKAVELFAFDISLEKL
                     RDELESSLMIHFGKRIQINGKNVLRIPNTSNIEFPFKAADFIRQTKTKLAVSTGSACT
                     SAENKPSHVLTAMGLSKENAERCIRFSLGKYTTQADIQSTIEIIKQIQVIKND"
     gene            698..1243
                     /locus_tag="PLF48_06430"
     CDS             698..1243
                     /locus_tag="PLF48_06430"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNF48998.1"
                     /translation="MKIYTKKGDDGTTTLYGGMRLSKGDLRIEAYGTVDELNSCIGVV
                     VSGLTDDAQQALFTSIQSRLFDIGTHLAAEPGKKNLMLPEINESMITLLENEIDKMNE
                     HLPELKHFILPGGNTVSAHCHVARTVCRRTERCVVRLAEHENTEPILIKYLNRLSDFL
                     FVFARKLAHDAGCPEKIWISN"
     gene            1342..1563
                     /locus_tag="PLF48_06435"
     CDS             1342..1563
                     /locus_tag="PLF48_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNF48999.1"
                     /translation="MYWTLELASYLEEAPFPASKDELIDYAIRTGAPLEVIENLQEME
                     DDEEVYESIEDIWPEYPTKEDFLFNEDEY"
     gene            complement(1726..2592)
                     /locus_tag="PLF48_06440"
     CDS             complement(1726..2592)
                     /locus_tag="PLF48_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNF49000.1"
                     /translation="MKKIATIFILNLMCVLLTSAQVEYGVASYYGIEFEGRPTASGET
                     YSQNKLTAAHKTMKLGTIVKVTNTQNNKSVYVKINDRGPYIKGRVLDLSTKAAHLLGY
                     RNAGTCTVKIETVHPNDIPKDLLAASVKLNKKPDLEKTPENDSAENRDETWKNIPTVD
                     TDIFNPYAPTGNQSSSSNSNAPANEWKLKGTEAIGNRKPYFVITKHNKENDGFFGIQL
                     GVFSDPTIIFEIADSICNDYDHEVIIEQSTLNDKTVYKLYIGKFQNKTYADALKLSMT
                     DKFKDAFVVRYQ"
     gene            complement(2640..3287)
                     /locus_tag="PLF48_06445"
     CDS             complement(2640..3287)
                     /locus_tag="PLF48_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49001.1"
                     /translation="MSNTTFISVEKTARIFTYGNLTNKTKLIWMVAHGYGQLAAYTIK
                     KFEVLHPEEHFVIVPEALNRFYLDGLSGRVGASWMTTEDREHEIADYISYLEKVYETL
                     ILHSGVKVVAFGFSQGASTICRWALQTQKPLNNIVLWGGSVPNECLEQVDQLNKLAPY
                     LLVGDADIYISEERKEEIMKNLRNIGLTFSHIFYKGGHSIVTEPLIELSARLTQT"
     gene            3376..3903
                     /locus_tag="PLF48_06450"
     CDS             3376..3903
                     /locus_tag="PLF48_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49002.1"
                     /translation="MKKLLFLACCMYLFAAHADSLTYSIKIKTIDLSEDLAPGATFND
                     ELVLLLYKKSVNIENPLPLVREYFVADSSHKTKIYSFKTDSLLSTDTLRLVLIEQDTR
                     KQLKAFDAICRVYFKELLKNKKGYYEFIDDDDMLGIAEIPAKTLLDKKTFIKKFETLN
                     LFDWAVYKIEINQIE"
     gene            4029..6890
                     /locus_tag="PLF48_06455"
     CDS             4029..6890
                     /locus_tag="PLF48_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF49003.1"
                     /translation="MFKLLFASLLLILFVLPSNGQSYKISGRITDSKDSSEIIGANIL
                     LNGIRDTTYKLGTVSDMNGNFELDNVPQGAYLLEFSYISYKTFTKKIFVKDNIQLEPI
                     QLEEDSKMLNEVNIEGKQIRVLQKGDTAQYNAEAFKTNKDATTEDLLMKMPGMSSENG
                     TVKVNGEEVKKVLVDGKAYFGDDTRTALQNLPAEVIDKIQVFDKMSDQAAFTGFDDGN
                     AQKTLNIITKNGLGNSKLGRFYAGYGGPDNRYTVGANYNDFKNDRRFSILAMSNNINI
                     QNFSVQDLVGAMGSSSGGGGRGGGRGGPSGMGRNSPLNNLMVFPQSGISTTTALGVNY
                     ADKWGKKKKVNFSGSYFFNASKNTNNSNTNRHYLSSNDSNLVYLENKEAVSKNFNNRL
                     NFRIEYTIDSNNTLIFTPSFTTQYYNNRSAMQANTNNAESILSTLQNEQHTKQQGYTF
                     NNEILYQHKLHKKGRTISASLQTNVNTKKNDGSLWSLNNYQQDTLEVTDSLDQQSSLK
                     TLNYTLGGSIVYTEPIKKTGQISLSYNPSYTRSASDKNTYNFDALSDSYTELDSTLSN
                     LFLNKYITNSLGIAYRFNKNKISWAVGVNGQSALLSSVQQFPYDFQIKKHFITALPNA
                     EFTYKFSKTDNLRINYRTSTNPPSISQLQNVIDNSNTLLLSTGNPELKQTFSQQISLR
                     YGRSNTDKATNMFIFAMFNNTRNYISTATQTIINDTLIQQMLVNNGTQLSYPVNMHGY
                     LAARTFITYGFPISKIKCNMNFNTGFAYTRTPTLINLLQNNANTYAVNGGFVVSSNIS
                     KKIDFTLIYAGSYNIVRNSLQQQNNSNYYSHTASAKINYQFWKGFVLNTGVSNQLNAG
                     GSSSYNVSYWLLNASLAYKFLKDESLELKFSANDILNQNKSISRNVTENYTEDVQSTA
                     LQRYFMGTITYTLRKTKGGAKQTDDKPKEFMPPPHPPGM"
     gene            complement(7330..7441)
                     /gene="rrf"
                     /locus_tag="PLF48_06460"
     rRNA            complement(7330..7441)
                     /gene="rrf"
                     /locus_tag="PLF48_06460"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7495..>7649)
                     /locus_tag="PLF48_06465"
     rRNA            complement(7495..>7649)
                     /locus_tag="PLF48_06465"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2598 a         1377 c         1381 g         2293 t
ORIGIN      
        1 ctgatattat tcatcaaaaa aactccatct tcttttgcga tgcgacacaa gccatgggga
       61 aaattcctgt tgatgtggat aaagatggaa tagatatgct atgcataagc ggtcataaaa
      121 tttatgcacc aaaaggtgtg ggatgtttgt ataccagacg caaaaacccg agagtaagcc
      181 ttgaacctct cctacatggc ggagggcatg agcaaggaaa aagaagcggc actttgaata
      241 tacctggcat tgtggctctc ggaaaggcag tagaactttt tgcgttcgat atttccttgg
      301 aaaaactccg ggatgaatta gaatcatctt taatgataca tttcggaaag cggatacaga
      361 ttaatggaaa aaatgtactg cgaattccaa atacttctaa tattgaattt ccatttaaag
      421 cagcagattt catacggcaa acaaaaacaa agctagccgt atcaacaggt tcagcctgta
      481 cttctgcaga aaataaaccc tcacatgtac ttactgccat gggcttaagc aaagaaaatg
      541 ctgaacgatg tattcgtttt agtttgggaa aatataccac acaagcagat atacaaagta
      601 caattgaaat aattaaacaa attcaggtga taaaaaatga ctagcacttt tttgcaacca
      661 aaaactcttt tattcatcta taaaatgacc ggtaattatg aaaatataca cgaaaaaagg
      721 cgatgatgga acaacaacac tttacggtgg catgcgctta tccaagggcg atttgcgcat
      781 tgaagcatac ggtacggttg acgaactcaa ctcatgcata ggagtagttg taagtggatt
      841 aacagatgat gctcaacaag cgctctttac ttctatacaa agccgattat ttgatattgg
      901 cacacattta gcggcagaac cgggaaagaa aaacctcatg ttgcctgaaa taaatgaaag
      961 catgattacg ttactcgaaa atgaaataga taaaatgaat gagcacttac ccgagttgaa
     1021 acattttata ttgcccggtg gaaatactgt atcagcacat tgccatgtcg cccgtactgt
     1081 ttgccggaga actgaaagat gtgtggtaag actggcagaa cacgaaaaca cagaaccgat
     1141 tttgataaaa tacttaaacc gcttgtctga ctttttgttc gtttttgcca gaaaattagc
     1201 ccatgatgca ggttgtccgg agaaaatctg gataagcaat tgataatcaa catcaaaatc
     1261 gttaaaaaaa atattttgtt tttttaaaaa agcgtatata tttgcaaccc tgaaaaaata
     1321 gtaaatctaa aatttaaaat tatgtactgg acactcgaat tagcatcata cctggaagaa
     1381 gcaccatttc ctgcatcaaa agatgaactt attgactatg ccatccgcac aggtgcacct
     1441 ttggaagtga ttgaaaactt acaggaaatg gaagatgatg aagaagttta tgaaagcatc
     1501 gaagacattt ggccggaata cccgacaaaa gaagatttct tgtttaatga agatgaatat
     1561 taatccaata gtcttattca aaaaattaaa gccgctcctg atgaagcggc tttttatttg
     1621 gctggtggtt acttttttaa tctgagtcgg gttaacaacg attcaattta attatagcac
     1681 aatggaatgt agtgaagatt gactatcctg aaaatactta cggttttatt gataacgtac
     1741 aacaaatgca tctttaaatt tatcggtcat gcttaatttg agcgcatctg cataggtttt
     1801 attttggaat ttaccgatat atagtttata tactgtttta tcatttagcg tagattgctc
     1861 aattattact tcgtggtcat aatcattgca aatactgtct gctatttcaa aaatgatagt
     1921 tgggtcagaa aacactccca gttgtatgcc aaaaaatccg tcattttctt tgttgtgttt
     1981 agtaatgacg aaataaggct ttcggttgcc aatagcttca gttcctttca gtttccattc
     2041 gttggcaggt gcatttgagt ttgaagaaga agattgatta cctgtcggag cataagggtt
     2101 aaaaatatca gtatctactg taggaatatt tttccatgtt tcgtctctgt tttctgcact
     2161 gtcgttctct ggcgtttttt ccaaatccgg ttttttattg agcttcacag aagctgccaa
     2221 taaatctttt ggaatatcat tgggatgcac ggtttcaatt ttcacggtac aagttccggc
     2281 atttctgtaa cccaataagt gggctgcttt ggtagataaa tccaacactc tgccttttat
     2341 ataaggtccc ctgtcgttta tttttacata aaccgactta ttattttgtg tgttggttac
     2401 tttaactatt gtgccgagtt tcatggtttt gtgtgctgca gtcaatttat tctgagaata
     2461 agtttcacct gaagcagtgg gtcttccttc aaactcaata ccataatacg atgcaacgcc
     2521 gtactctacc tgagctgatg tgagtaatac acacatcagg tttaatataa atatggtggc
     2581 aatttttttc atgcatttcg tttcgttcaa aaagctgatg caaaaataag gcggtaatgt
     2641 taggtttgtg ttaagcgggc agagagttcg attaaaggtt cggttacgat agaatgaccg
     2701 cctttgtaaa aaatatgtga aaatgttaag ccaatattac gtaaattttt cataatttct
     2761 tcttttcttt cttcacttat atagatatct gcgtcgccta ccaataaata aggagctaat
     2821 ttgtttaatt ggtcgacttg ttctaaacac tcattaggaa cgctacctcc ccataaaacg
     2881 atgttattca acggtttttg tgtttgtaaa gcccatctgc atattgtact cgccccttga
     2941 gagaagccaa aagccaccac ttttacacct gaatgtaaaa ttaatgtttc gtaaactttt
     3001 tctaagtatg aaatatagtc ggcaatctca tgctcccgat cttctgttgt catccaactg
     3061 gcacctaccc taccggataa gccatccaaa tagaaacggt tgagtgcctc gggtacaata
     3121 acaaaatgct cttcaggatg cagtacttca aactttttaa ttgtgtaagc ggctaattgt
     3181 ccgtacccat gtgctaccat ccatatcagc ttggtcttat tggtcagatt gccatatgta
     3241 aaaattcttg ccgttttttc gactgaaata aaggtagtgt tagacatata gtatgaaatt
     3301 agttaaaatg tgctatttta aaagctaaaa taaactaacg ttccggtgaa attgttaata
     3361 cataaaaatt caactatgaa gaaactactt tttttagcct gttgcatgta tttgtttgca
     3421 gctcatgctg attcgctgac ttacagcata aagattaaaa cgatagattt gagcgaagac
     3481 cttgcaccag gagctacttt caatgatgag ctggttttat tgttgtataa gaaatcagtt
     3541 aacattgaaa atccacttcc attggtacgc gagtattttg tggctgattc atcgcacaaa
     3601 acaaaaatat atagctttaa aacagactcc cttttaagta cagataccct gcgtctggtt
     3661 ttgatagaac aagacacacg caaacaactg aaagcctttg atgccatctg tagggtttat
     3721 ttcaaagaat tattaaaaaa caaaaaaggg tattacgaat ttattgatga tgatgatatg
     3781 ctgggcattg ctgaaatacc tgcaaaaacg ctgttagata agaaaacatt tatcaaaaaa
     3841 tttgaaactt tgaatctttt cgactgggca gtttataaaa ttgaaatcaa tcaaatagaa
     3901 tagcttccag caaataaaat ctattatctg aaaattgaat gcttaataaa gattaaacaa
     3961 atctgaaatt gcaacttcat attaaacttt acccgatatt tacagtctaa tatctactct
     4021 tgtcatttat gtttaaactt ttatttgcct cactattact tattttattt gttttaccct
     4081 ctaatgggca gtcctataaa atttcaggaa gaattacaga tagcaaagac agttcagaaa
     4141 ttattggcgc aaatattctg cttaacggta ttcgggatac cacttataaa ctgggcacag
     4201 tatctgatat gaatggaaac tttgaacttg ataatgtacc gcaaggtgct tatttgcttg
     4261 agttttctta tatcagctat aaaactttta cgaagaaaat ttttgtaaaa gacaatattc
     4321 aacttgagcc tatacagctc gaagaagatt caaaaatgct gaatgaagtc aatattgaag
     4381 gtaaacaaat tcgtgtgctg caaaaaggag atacagcaca atacaatgcc gaggcattta
     4441 aaactaataa agatgcaact actgaagacc ttttaatgaa aatgccgggt atgtcctcgg
     4501 agaacggaac tgtaaaagta aatggtgaag aagttaaaaa agtattggtg gacggaaaag
     4561 cctattttgg cgatgataca cgcactgcat tgcaaaacct acctgctgaa gtaatcgata
     4621 aaattcaggt ttttgataaa atgagtgatc aggccgcgtt tacaggattt gacgatggca
     4681 atgcacaaaa gacattaaac attataacta aaaatggttt aggcaactcg aaattaggta
     4741 gattttatgc gggatatggc ggaccggaca accgctatac agtgggtgcc aactataatg
     4801 atttcaaaaa cgaccgcaga ttttccattc ttgccatgag caataatatc aatatacaaa
     4861 attttagcgt gcaagattta gttggtgcaa tgggaagttc atccggtggt ggcggaagag
     4921 gcggaggcag aggcgggcca agtggaatgg gcagaaacag cccactgaac aatttaatgg
     4981 tatttccgca aagcggtatc tctactacta ctgcattagg ggtaaattat gcggataaat
     5041 ggggtaaaaa gaagaaagtc aatttctcgg gttcttattt ttttaatgct tcaaaaaaca
     5101 caaataacag caataccaac agacattatt taagttcaaa tgacagcaat ctggtatatc
     5161 ttgaaaataa agaagcagtt tcgaaaaact ttaacaatcg tcttaatttc agaatcgaat
     5221 ataccataga cagtaataat acactgattt ttaccccttc atttaccaca caatattaca
     5281 acaaccgctc tgccatgcag gcgaatacca acaatgctga aagcatactc agtacattgc
     5341 aaaacgaaca acatactaag caacaaggct acacattcaa taatgaaata ttgtatcagc
     5401 ataaattgca taaaaaaggc agaaccattt ctgcaagctt acaaaccaat gtgaatacca
     5461 agaagaatga cggctcattg tggagtttga ataattatca gcaagatacg ttagaggtaa
     5521 cagatagcct ggatcagcaa tcatccttaa aaacacttaa ctacacacta ggcggatcaa
     5581 ttgtatatac tgaaccaatt aaaaaaaccg gacaaatctc attgtcctac aacccttctt
     5641 atacacgttc agcatccgac aaaaacacgt ataatttcga tgcactatct gattcctata
     5701 cggagttaga ctcaacttta agcaatttgt ttttaaataa gtatatcacc aatagtcttg
     5761 gcattgccta tcgtttcaac aaaaataaaa taagctgggc ggtcggtgtc aatgggcaaa
     5821 gtgcacttct gagcagtgta cagcaatttc cctacgattt tcaaataaaa aaacatttta
     5881 ttaccgcctt gccaaatgca gaatttacct ataaattttc caagacagat aatcttcgaa
     5941 tcaattatag gacttcaacc aatcctccaa gtatttcaca attgcaaaat gtaattgaca
     6001 acagcaatac tttactctta agtacaggca accccgaatt gaaacaaact ttttctcagc
     6061 aaatcagctt aagatacggc agatcgaata cagataaagc aacaaacatg ttcattttcg
     6121 ccatgttcaa caatacccgg aattatattt ctacagccac acaaactata ataaatgata
     6181 cactcattca gcaaatgctt gtcaataatg gtacacaatt aagctatccg gtaaacatgc
     6241 acggctatct ggcggcaaga acatttatta cgtatggttt tccaatatca aaaataaaat
     6301 gtaatatgaa tttcaacacc ggttttgcat atacccgtac acctacgctt attaatctgt
     6361 tgcagaataa tgcaaacact tatgctgtaa acggaggttt tgtagtgagt agcaatatta
     6421 gtaaaaaaat agattttacc ttgatctatg ccggttcata taatatagta cgcaattcac
     6481 tgcaacaaca aaataattcc aattactatt cacatactgc atcggcaaaa atcaactatc
     6541 agttttggaa aggcttcgta ttgaataccg gagtaagtaa tcagctgaat gccgggggta
     6601 gttcgagcta taatgtaagt tactggttgc tcaatgcaag tttggcttac aaattcctga
     6661 aagacgaaag tctggaactt aaattcagtg ccaatgatat cctgaatcaa aataagagca
     6721 tcagtcgcaa tgtaaccgaa aattatactg aagatgtaca aagtacggca ttgcaacgct
     6781 attttatggg tactatcact tatactttgc gaaaaacaaa aggaggtgca aaacaaacag
     6841 acgataaacc aaaagagttt atgccacctc ctcatcctcc gggcatgtaa cacctcgtag
     6901 gttaaaaatc aaacacattg ataatcaaca ccttttgaat ttctaaatag aaattgacct
     6961 cgtaggttgg ttttggtgat tgtattatat accagaaaat tagatattgt cgtaacaaac
     7021 gaagttaaaa aaagtcttca aattaatccc ataggtatat ttgctcacaa ctcatacctg
     7081 tatttactgg catgtatcgt aagttaaagt ttctttgtga tgcaaaactt gaaattactt
     7141 tttattttct taattcaata ggttttagta catgcccaaa ggcaacatgt aatgaaactt
     7201 atgccttagt agtaatgtag ttgaatatca gaatactttt gagacaagac agaacaatca
     7261 tacagtataa aaaacatgaa gtaaaagagg taaaataaaa aaaccccgca agaatgcggg
     7321 gctgtaagag attggcgacg acctactctc ccaggtttta acctagtacc atcggcgctg
     7381 tggggcttaa cttctctgtt cgggatggga agaggtgaac acccacgcta aaatcgccat
     7441 taaaattttt agcgtaatgc tgtatatttt aacatattgg gatattattc aaattttaaa
     7501 aagttgatgg gttattagta ctactcggct ttgttgttac caactttaca cctatagcct
     7561 atcaacgtcg tagtcttcga cgaccctatg agaactcatc ttgaagtggg tttcgtgctt
     7621 agatgctttc agcgcttatc ccttcccaa
//
LOCUS       DAOQII010000107         7641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5228_length_7641_cov_4.598471, whole genome shotgun sequence.
ACCESSION   DAOQII010000107 DAOQII010000000
VERSION     DAOQII010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_7641_cov_4.598471"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..923)
                     /locus_tag="PLF48_06470"
     CDS             complement(48..923)
                     /locus_tag="PLF48_06470"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNF49004.1"
                     /translation="MSEELHQKVPKKKKPSFIYAIISIAMILFIIGLFSSSVLFTRSY
                     YNKLKESIQIDIYLKDDISDSAKNSLTQYLEHQRFLSKVKYISKDESAQEFKKAYNQD
                     FEQILGNNPLPAAFRINLKAENSNEEFAKDLKYKLQGMDGVQEVYYNALAFKTVGTQI
                     NPIFWGILVLCLVLLIVAFLIIDNTIKLLMYSQRNTIRTMQLIGATDGFIIKPYIWRS
                     AVIGIISAIVAILILALLIYLTIYKFSLEISATDFVNLSLIALGLVVFGILIAVISTY
                     FSVNKYLKVKLDELY"
     gene            1002..1709
                     /locus_tag="PLF48_06475"
     CDS             1002..1709
                     /locus_tag="PLF48_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNF49005.1"
                     /translation="MNNAPYGIELYLLPTYFIDSDHPDIIRFVKNTIGKEIDKKKIAV
                     KLYQAVRDGWKYNPYNINLQHKELKASTLFNRENKSGYCIEKAGLLAACLRAADIPAR
                     LCFFDVKNHIGVEKFIDLIKTDILAFHACTDVYLDEKWVKATPAFNKELCDKLEVEVL
                     EFDGEQDSVFQQFDKNGSVFMEYVKEYGSFHDVPHDMFVMLMKQYYPHFFSEKKQDKI
                     MYGKEAESIPSKNGKSK"
     gene            1721..2452
                     /locus_tag="PLF48_06480"
     CDS             1721..2452
                     /locus_tag="PLF48_06480"
                     /inference="COORDINATES: protein motif:HMM:NF028434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNF49006.1"
                     /translation="MQPLKKILIPIPDKDFDTTEVALAWEIFKKNHFHVVFATEHGKA
                     GETDPLLLSGVIFGQLGAKKNAIAAYHEMQKSGEFLNPITYFHIKPQEFDLLYLPGGH
                     AKGMRQYLESKVLQDRIVEFFKLNKLIGSVCHGSLVMARSIDPATGKSIVYDYTISGL
                     PKSLEMLAYGLTSWKLGDYYKTYPEYLQDEVERLQKTKKQFKTGLSSFIPFVVEDRNL
                     TCARWPNDVEKLANRLVERLKSVQY"
     gene            complement(2528..3490)
                     /locus_tag="PLF48_06485"
     CDS             complement(2528..3490)
                     /locus_tag="PLF48_06485"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HNF49007.1"
                     /translation="MKKIFMMFGAVAMLFTACQKSGFTNQDDQTGMPNQRTCGSVQVL
                     EEQLKADPTLQAKMDAFEKNVEANQKNGVTARLVNGVIEIPVVVNVLYRTTAENISQA
                     QIQSQIDVLNEDFNATNADYSLVPSTFSGVKANVGIRFVLDQVIRKSTNKTSWRTDDA
                     MKKSTKGGINPTSPTTKLNLWCCTLSGGILGYAQFPGGSSATDGVVILNTAFGRTGTV
                     LAPYNKGRTATHEVGHWLNLRHIWGDATCGSDLVSDTPVHNTANYGCPAAGHKSTCTG
                     TPVEMTMNYMDYTDDACMYMFSAGQKSRILATFATGGPRNSFAQ"
     gene            3865..5055
                     /gene="hflX"
                     /locus_tag="PLF48_06490"
     CDS             3865..5055
                     /gene="hflX"
                     /locus_tag="PLF48_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNF49008.1"
                     /translation="MIEINPHIEERAILVGLIRDNQTAEKVDEYLDELAFLAETAGAV
                     TVKKITQRLKHPDTRYFVGLGKMEEIKQYMEFSGATTVIIDDEISPAQQRNLEEFLKC
                     KILDRTSLILDIFAMRAQTAQARSQVELAQLQYLLPRLKGLWTHLERQRGGIGMRGPG
                     EKEIETDRRVINDKIAKLKKDLLVLDKQEETRRKTRGELIRVALVGYTNVGKSTIMNS
                     ISKSEVLAENKLFATLDTTVRKVVIDNIPFLLSDTVGFIRKLPHHLVESFKSTLDEAK
                     ESDIIVHVVDAGHENFRDHILVVNETLAELGVTQQPRLMVFNKMDIYRERHFDKFLPE
                     EVKHDLTREFEKNMRNQMNANCVFISATNKENMDEFRQVLALMVQDMYHKRYPYRSNY
                     FDLH"
     gene            complement(5139..7094)
                     /locus_tag="PLF48_06495"
     CDS             complement(5139..7094)
                     /locus_tag="PLF48_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNF49009.1"
                     /translation="MADNSSHHGIHKLSAAGVIVALGIIYGDIGTSPLYVLKAILGMH
                     AGPIDPNIVYGGVSCVFWTLTFIVTIKYVYLALNADNKGEGGIFALYALVRRYKAKWT
                     LIPALIGCASLMADGFITPPISISSAIEGLNILYPHMETVPIVIAILTGIFVLQQFGT
                     HKIGNAFGPVMFLWFIMIAVLGLVQIVKSPDVLKALNPYYAINLVLGYKGGIWFLGAV
                     FLCSTGGEALYSDLGHCGKQNIRVSWGFVKIALLLNYFGQAAFVVKNPGINYAEVAPF
                     YALMPAWFLKIGIAIATVATIIASQALISGCFTLVNEAMKLKLWPNQKVIYPSLVQGQ
                     IYIPFINWLLFVGCLAVVLIFRESSKMEAAYGLAITLNMLMTTSLLTYYLHVKRKPAL
                     LVWTFFTFYMWLEITFLTANLSKFSHGGWFAVLIAIAIFVVMYLFYLGKKLRNKHIEF
                     VEIKDYMDDLVDLQNDHTLPKEATNLVFMCNANDKKHIDSNIIYSIFRKKPKRADTYW
                     FVHVDITNEPYGASYSVDTIVPKKCFFIRLKFGFKVEHKVHVMFSRIVEEMEKRGEID
                     LLSHYPSLRKHGYPADFKYVVLNSLVSIDNQLSPWEQFLIKSYNSIKNLSIPAEVDFG
                     LEKTNCRVENIPIRISHKTEIDIHREE"
     gene            complement(7269..7604)
                     /gene="secG"
                     /locus_tag="PLF48_06500"
     CDS             complement(7269..7604)
                     /gene="secG"
                     /locus_tag="PLF48_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNF49010.1"
                     /translation="MFLSILIIIVSLFIVMVVLVQSPKGGGLTGGLGSTTSNIIGVQS
                     AGDVMEKVTWGSAIVLLVLCVLTAFSLPKNQDPSKAKTEVEQAVANSAAAPAAPVAPQ
                     ALPQQTPAK"
BASE COUNT         2487 a         1336 c         1444 g         2374 t
ORIGIN      
        1 aatggaatgt ttttttgtac cggtttattt tttgccataa tgcctgttta atacaattcg
       61 tcaagtttta ctttcaggta tttgtttact gaaaaataag tgctgattac agcaatcaaa
      121 attccgaaaa cgactaaccc taatgctatg agcgacaaat ttacaaaatc tgtagctgaa
      181 atttccagag aaaacttata aattgttaaa taaatgagca aagccaggat aagtatggca
      241 actattgcac tgataatgcc gataactgcc gaccgccaga tataaggttt gataataaaa
      301 ccgtccgttg ctcctattaa ctgcatagtg cgaatggtat tgcgttgcga atacatcagt
      361 aatttgatgg tgttatcgat aatgagaaaa gctacaatca ataagactaa acaaagcacc
      421 agaatacccc aaaaaatggg attaatctgt gtccccaccg ttttgaatgc cagcgcattg
      481 taatagactt cttgcacgcc atccatgcct tggagtttat attttaagtc ttttgcaaat
      541 tcttcgttag agttttctgc ttttaaatta attctaaacg ctgctggaag cggattattt
      601 cctaaaattt gttcaaaatc ctggttataa gctttcttga attcttgggc tgattcatct
      661 ttgctgatat attttacctt tgataaaaaa cgctgatgct ccaaatattg tgtcaagctg
      721 tttttagctg aatcggaaat gtcatctttc agatagatat caatctgaat actttctttc
      781 agcttattat aataacttcg ggtaaataat acactgctgc taaataaacc aatgataaac
      841 aaaatcatag cgatactgat aatcgcatat ataaaactcg gttttttctt tttgggtact
      901 ttctgatgta attcttccga catgccaacg aaaatagtta ttttttaatt acttaatacg
      961 caaggttatt cacagcttgt gtgaacccgt atttttacgt tatgaataac gccccttacg
     1021 gaatagaatt gtatttgcta cctacctatt ttatagatag tgaccatcct gatattatac
     1081 gttttgtaaa aaatacaata ggaaaagaaa tagacaaaaa gaaaattgct gttaaattgt
     1141 atcaagccgt tagagacgga tggaaataca atccatacaa tatcaatttg cagcataaag
     1201 aacttaaagc cagtacactg tttaaccgtg aaaataagag cggctactgt atagaaaaag
     1261 caggcttact cgctgcttgt cttcgggctg cagatatacc tgcaaggtta tgtttttttg
     1321 atgttaagaa tcatataggt gttgaaaagt ttatagattt aatcaaaacg gatattctgg
     1381 catttcatgc ttgtactgat gtttatttag atgaaaaatg ggtaaaggct acccctgcat
     1441 tcaataaaga attatgcgac aaacttgaag tggaagtact cgaatttgat ggagaacaag
     1501 attccgtatt tcagcaattt gataaaaacg gcagcgtatt tatggagtat gtcaaagaat
     1561 atggttcttt tcatgatgtg ccgcatgata tgtttgtgat gctgatgaaa cagtattacc
     1621 cccacttttt cagtgagaaa aaacaagata aaatcatgta tggaaaagaa gcagaaagca
     1681 tcccttcaaa aaacggtaaa agcaagtaaa atttaatatc atgcaaccgc taaaaaaaat
     1741 attgattcct atacctgata aagattttga tacgacggaa gtagcgctgg catgggaaat
     1801 attcaagaaa aatcattttc atgttgtatt tgctacagaa cacggtaaag caggagagac
     1861 agacccgtta ttgctttccg gtgtgatttt tggacaatta ggcgcaaaga aaaatgcaat
     1921 agcagcttat catgaaatgc aaaagtcggg tgaatttctg aatccaatta cctatttcca
     1981 tatcaaaccg caagaatttg atttgttgta cctgcccggc gggcatgcta aaggcatgcg
     2041 gcaatacctt gaaagcaaag tgttgcaaga tagaattgtc gagtttttta aactcaacaa
     2101 attaattgga agtgtgtgcc acggcagttt ggttatggca cgaagtatag accctgctac
     2161 cggaaaaagt attgtgtatg attatacaat ttccggcttg cccaaatctt tggaaatgct
     2221 ggcgtatgga cttacatctt ggaaattggg cgattattac aaaacctatc ccgagtattt
     2281 gcaggatgaa gtggaacgtt tacagaagac taaaaagcaa ttcaaaacag gcttaagcag
     2341 ttttattcca tttgtagttg aagatcgtaa tctgacttgt gcccgctggc ccaatgacgt
     2401 ggagaaatta gctaaccgct tggtagaaag actcaaatca gtgcagtatt aactaattca
     2461 tgcaatataa aaaaaaagag gctaccgaat tggcagcctc ttttttattt tagtatttga
     2521 atattgctta ttgagcaaaa gagttacgtg gtccacctgt agcaaaagta gctaagattc
     2581 tagatttttg accagcactg aacatataca tacaagcatc gtctgtatag tccatatagt
     2641 tcattgtcat ttctaccgga gtacctgtac aagtagattt gtgacctgca gccggacagc
     2701 catagtttgc agtattgtgt accggagtat cagaaactaa gtcacttccg caagttgcat
     2761 caccccagat atgacgaagg tttaaccagt gacctacttc gtgagttgca gtacgtcctt
     2821 tgttatatgg agctaatacc gtgccagtac gaccaaatgc agtatttaaa attacaacac
     2881 catctgtagc agaagaacca cccggaaact gagcataacc taagataccg ccactcaaag
     2941 tacagcacca taagtttaat ttagttgttg gtgaagttgg attaatacca ccttttgtag
     3001 attttttcat ggcatcatca gttctccaag atgttttatt agtagatttg cgaataacct
     3061 gatctaaaac gaaacgaata cctacattag cttttacacc tgaaaaggta gaaggaacta
     3121 aagaataatc agcatttgtt gcattaaaat cttcattcaa tacatcaatt tgagattgaa
     3181 tttgagcttg agaaatgttc tcagcagttg ttctgtataa tacatttacg actaccggaa
     3241 tttcaattac tccgtttact aaacgagcag ttacgccatt tttttgatta gcttccacat
     3301 ttttttcaaa tgcatccatt ttagcttgta aagtagggtc tgcttttaat tgttcttcca
     3361 atacttgcac agaaccgcaa gttctttgat ttggcatgcc ggtttgatca tcttgattag
     3421 tgaaaccgga tttttgacat gctgtaaata gcatagctac agcaccgaac atcataaaga
     3481 ttttcttcat tggatttaat ttttttgttg tttacgttat taaatgtaat tcaaacttaa
     3541 taaacatttt ttgtgaaaca aaatttttta acaattttct ttgttaagcc ggttttttct
     3601 gacttttctg ttatttattt aaatctgagt tcgggaatta catttctatt aaaaatagtt
     3661 gatgtttttt aacaagcacc atcatctttt aattcctctt cttgagttaa gttagagcaa
     3721 aaaataagaa gtcaattgta tttttacttg atttttctta actgaactca ggtttttaat
     3781 ctatctcaaa aaaactatct ttgcttttat tataaaatca acattgtata ttgctatgca
     3841 gtgcgtcaaa ttaattaatc tgttttgata gaaataaatc cacatattga agagcgtgcc
     3901 attctggtag gattgattcg cgacaaccaa accgccgaaa aagttgatga atatttagat
     3961 gaattggcat ttctggcaga aactgcagga gctgtaacgg tgaaaaaaat tactcaacgc
     4021 ttaaaacatc ccgatacccg ttattttgta ggcttgggta aaatggaaga gattaagcaa
     4081 tacatggagt tttccggtgc tactacggta attattgatg atgaaatttc gccggcccaa
     4141 cagcgcaatt tggaagagtt tctaaaatgt aaaattttgg atcgcactag tttgatattg
     4201 gatatttttg ctatgcgggc acaaacggca caagccagaa gtcaggtaga actggcacag
     4261 ttgcaatatt tgttgccacg gctaaaaggt ttgtggactc acctagaaag gcagcgtgga
     4321 ggtattggta tgcgcgggcc gggtgagaaa gaaattgaaa ccgacagaag ggtaattaat
     4381 gataagatag ccaaactgaa aaaagattta ctagttttag acaaacaaga ggagacacgc
     4441 aggaaaaccc ggggggaatt gataagagta gcattagtgg gttataccaa tgtaggtaaa
     4501 tcaaccataa tgaacagcat cagtaagagt gaagtgctgg cggaaaacaa actgtttgct
     4561 accttagata caacggtgcg aaaagtggtg attgataata tccctttttt attgagtgat
     4621 acagtgggct ttattcggaa attgccacat catttagttg agagtttcaa gtcgactttg
     4681 gatgaggcaa aagagtcgga tattattgtc cacgtagtgg atgcgggaca cgaaaatttc
     4741 agagatcata ttcttgttgt taatgagaca ctggcggagc tgggcgttac gcaacaaccg
     4801 cgtctgatgg tatttaacaa gatggatatt tacagggaaa ggcattttga taaattcctt
     4861 cctgaagaag taaaacatga cttaaccaga gaatttgaaa aaaatatgcg caatcagatg
     4921 aatgcgaact gtgtttttat ttccgcaacc aataaggaaa acatggacga attcagacag
     4981 gttttagcgc tgatggtaca agatatgtat cataaaagat atccttacag aagcaattat
     5041 tttgatttgc actgaaatat cagaactata ctgtttacat ttaacccgaa ctcaggttaa
     5101 tacatcaacc cgaaagaaat ctatactaca ggaagtattt attcttctct atgaatatca
     5161 atttctgttt tatgggatat tctaatcgga atattttcta ctctgcagtt ggttttttcc
     5221 aaaccaaaat ccacttctgc cggaatactt aagtttttta tcgaattata agattttatt
     5281 aaaaactgct cccaagggct gagttggttg tctatggaaa ctaatgaatt aagtacaacg
     5341 tatttaaaat ctgccggata tccatgttta cgcaaagacg gataatggct gagcaagtcg
     5401 atttcaccgc gtttttccat ttcctctaca attcggctaa acattacatg tactttgtgc
     5461 tctactttaa atccgaattt taaccggata aagaaacatt ttttaggcac tattgtatct
     5521 acggaataag atgcgccgta aggctcattg gttatatcta catgtacaaa ccaataagtg
     5581 tctgcccttt ttggcttttt cctgaatatg gaataaataa tattagaatc aatatgcttc
     5641 ttatcgttgg cattgcacat gaatacaagg ttagtggctt cttttggtaa agtatgatcg
     5701 ttttgcaaat caaccaaatc atccatataa tctttgattt caacaaattc gatatgtttg
     5761 ttgcgcaatt ttttccccag atagaataaa tacatgacta caaaaatggc aattgcaata
     5821 agcacggcaa accagccacc atggctgaat ttgcttaggt tggcagttag gaaggtaatt
     5881 tcgagccaca tgtagaaggt gaaaaatgtc caaaccaaaa gtgcaggttt gcgttttaca
     5941 tgcaaataat aagtgagcaa actggtggtc atcagcatat ttaaagtaat ggctaagcca
     6001 taggccgctt ccattttgct tgattctctg aatatcagaa ccacagccaa gcaacctaca
     6061 aataataacc aatttataaa cggtatataa atttgacctt gaactaaaga cggataaatg
     6121 actttttgat tcggccaaag tttaagtttc atagcttcat tcaccaaagt aaaacagcca
     6181 ctaatcaatg cttgtgaggc aataattgta gccacagtag ctattgcaat tccgattttt
     6241 aaaaaccacg ccggcatgag tgcgtaaaag ggtgccactt cggcataatt tattccgggg
     6301 ttttttacta caaatgcagc ttgtccgaag tagttgagca ataaagcaat ttttacgaaa
     6361 ccccagctta cacgaatatt ttgtttgccg caatgtccta aatctgagta gagtgcttca
     6421 cctcccgtag aacaaagaaa tactgcgcct aaaaaccaga ttccgccctt atatcctaaa
     6481 acaaggttta ttgcataata gggatttaaa gcttttagta catccggact ttttacaatt
     6541 tgtactaatc ccagtacggc aatcataata aaccatagaa acataaccgg accgaatgca
     6601 tttccaattt tgtgtgtacc aaattgttgt aaaacaaata ttcctgtcag aattgcaatg
     6661 acaatcggaa cagtttccat gtgcggatat aagatgttca aaccttcgat ggctgatgaa
     6721 atggaaatgg gtggagtaat aaatccgtct gccatgagtg aagcacaacc tatcaaggca
     6781 ggaatgagtg tccattttgc tttgtaacga cgaacgagtg catagagagc aaatatacca
     6841 ccttcacctt tgttgtcagc attaagggcg agataaacat attttatagt aactataaaa
     6901 gttaatgtcc agaacacgca agatacacca ccgtacacaa tattcggatc tattggtccg
     6961 gcgtgcatac ctaagattgc tttaagcacg tagagaggag aggtaccgat atctccgtag
     7021 ataataccta atgcaacgat tacacctgct gccgaaagtt tgtggatgcc atgatgactt
     7081 gagttgtcag ccatttttag aaaaatttaa gttgcaaaat tacgatattt tatgctgtgg
     7141 gtataatatg aattaattat tttctttcgg cattatttta ttcaggttta agagcagtta
     7201 atgttttgaa taaaagaaaa aaagctcaca gagtgagctt tttattaatt accgaatgct
     7261 taagtgtatt atttagccgg agtttgttga ggtagcgctt gtggtgcaac aggagcagcg
     7321 ggcgcagctg cactattggc aactgcttgt tctacttctg tttttgcttt agatggatct
     7381 tggtttttag gcaaactaaa tgcagttaat acacacagta ccaataatac gatggcactg
     7441 ccccaagtta ctttttccat aacatcaccc gcactttgca caccgataat gttggatgtc
     7501 gtagaaccca aacctccggt aagtcctcca cctttgggac tttgaactaa cacgaccatc
     7561 acgataaata aactaacgat gatgattaat atagataaaa acaaaaacat aattattctc
     7621 ctttattgat tttttcgatt a
//
LOCUS       DAOQII010000108         7632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5244_length_7632_cov_5.086314, whole genome shotgun sequence.
ACCESSION   DAOQII010000108 DAOQII010000000
VERSION     DAOQII010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5244_length_7632_cov_5.086314"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLF48_06505"
     CDS             complement(<1..665)
                     /locus_tag="PLF48_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF49011.1"
                     /translation="MTAKISAPENKTGFSINEYPDIRPIYQKLDKLVALPLQHIQADA
                     MQNYLDYYKTKCTKSHEIYEQARQFIPGGVQHNLAFNFPFPLTFTKAEGAYLYDADGN
                     KYIDFLQAGGPTVLGSNYETVRLKAIELINECGPSTGLLHEYEYKLAELICKHIPSVE
                     QFRMLGSGTEAVMGALRLARIKTGKKKIIKMGGAYHGWSDQMVYDLRIPGTRFFDAKG
                     IPF"
     gene            736..1308
                     /locus_tag="PLF48_06510"
     CDS             736..1308
                     /locus_tag="PLF48_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF49012.1"
                     /translation="MNTRPHSFTDYLAKFGNLSPELIQSVDKKIKVSVFAKKTILLKA
                     GKVCEYLYFIEKGMARNISEEDDRVFTNDIVLEGEILVSFASFVSQKPSVESIELLED
                     STLSCLHYDDLQDLYTDFPELEKIGRKIAEYHYNSLAIKTHQLRFFSSTQRYQHLFDT
                     KIEIIKRAPLGIIASYLGMTIENLSRIRNK"
     gene            1405..2184
                     /locus_tag="PLF48_06515"
     CDS             1405..2184
                     /locus_tag="PLF48_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNF49013.1"
                     /translation="MHELLQHIIDNPLASITIILNLILIESLLSVDNAAVLATMVMDL
                     PKEQRGRALKYGIIGAYVFRGICLLFAALLIKIWWLKPLGGLYLLYLVWNWWRGKQTT
                     TVEDDIVDKKSNWFYRNTVGRLGIFWSTVVLVEIMDLAFSIDNVFAAVAFTDNILLVW
                     TGVFIGIMAMRLVAQGFVNLMEKYPFLESCAFLVIAILGIKLLLSVFEHLQPESAFSK
                     FLMSHEADWITSLLTAGIFFIPVLTSYLFNFPKKHEHKDQD"
     gene            complement(2336..3283)
                     /locus_tag="PLF48_06520"
     CDS             complement(2336..3283)
                     /locus_tag="PLF48_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNF49014.1"
                     /translation="MFKEKKVLVVGDIMLDEFVETQQSGFSQEYANNPILQVKHRNYY
                     LGGAANVALNCTKLEADVFLLGTTGVDKFQEILHELLRSNNISTEYVVANPKQHTTLK
                     TRVLQNGIPLLRIDEETTADFDKAIDDYLLFNFKEIITKQKPDVIIFQDYNKGVLNPY
                     LIPLFLELARENKIPVCVDPKFENWMLYQSVDVFKPNRKELAFMNQLETEPESDEDIV
                     RCAEFIRNQLNCKTVLVTLGSRGTYCLSEQDSFFTEPYKYIAHPDVCGAGDTVIAVIA
                     LAYASGYEWEKIAALANKAANIVCHKRQIQPVNWEELQS"
     gene            complement(3335..5296)
                     /locus_tag="PLF48_06525"
     CDS             complement(3335..5296)
                     /locus_tag="PLF48_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNF49015.1"
                     /translation="MSTDNHLRNKLSVSGLLITLGIIYGDIGTSPLYVMKAIIGNKPI
                     QPDIIFGALSCIFWTLIIVTTFKYVILVMQADNQGEGGIFSLYALVKKHQAKWPLIAA
                     LIGCSALLADGFITPPISISSAVEGMNILYPDLPTIPVVLTILFLLFIFQQFGTGTIG
                     KTFGPIMLLWFTTLGVLGLLKVIHHPEIFKALNPVYAFDLLFNHPGGFWILGAVFLCT
                     TGAEALYADMGHCGKRNIQISWIYVLACLLLNYMGQSIWCLQHRGISLNDVSPFYELI
                     PKPYLTFLIILATVATIIASQALISGCFTLVSEAIKQRLWINLKVDYPSDSKGQVYIP
                     VMNWVLMSGCIFIIILFKRSANMEAAYGLTITIDMLMTTSLLLLFFKIKNKSLAFIIP
                     VGVLFFSIESVFLMSNLKKFFYGGWFTFFIASVTFFILFCLHKARAIRDTYNNMVNLD
                     DFKGMFSDLIQDTTIPKTATNLVYMSKKSKRTSNVDSNIIYSIFQQNPKRADVYWFIH
                     VDTLDYPHEHEKNYYVKTIIPGKVFFVRLEFGFKVKHKVNRLFHKIVEHMVELGEVDE
                     LSRYPSMRQNNIKADFKFVFIKPKLSEDNDLKPNNLLVMNIYELLSKISYPPYKEFGL
                     DTVNTEIELTPLQIHHQEDLIVHRKEGFE"
     gene            5716..7323
                     /locus_tag="PLF48_06530"
     CDS             5716..7323
                     /locus_tag="PLF48_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49016.1"
                     /translation="MFFVLSFLAFYKGWNTGWNYDSIGFSYLAKKNSLSTLLSLHIDK
                     TSRFVYHTVYYLLNKYFGYQGWDWFLFKIALHAFNALLLLIVVKLILQYYASINNYAI
                     PILTALLFLLAPTTTEVVLYQAAIQFLFTYSLLYLCILLVIFSIRSGKKAFVWIYFAL
                     FFIALFSLETSYTFPAIAFLLVAFYPGIKIKSKQTVLVVLIPILFVLSVLFINYALFG
                     DSLSHYGQQKHLNISLDKLIQNMLKIPLDFIAFIEYWKMDYIYKVYNILYKKEAIYLF
                     AVLFILIYLSLWKKYLNDQLSKPFWMCIHILFVMILPTIALGILYIIPIEEDRYLYYP
                     SGMIFFCIVWILYKISPKLMYAGVACLLFIGFLFLQKNIYSWTITQKTIDYSLSSFPY
                     EKGKRYILLASPVYANGVLIWDTHETDKDPFEDKYQILGKALYVKQNIDVMDKIAPVL
                     QYNIINEKESPEISTLNDSTLKMTLPCCGKWWWKHNSGAQDMETGYYKIKMVGGWQQE
                     VHIQFKQKPKDLVYIYQCGTQFCKYDF"
BASE COUNT         2489 a         1323 c         1295 g         2525 t
ORIGIN      
        1 ataaaaggaa tgccttttgc atcaaaaaaa cgagttccgg gaatacgcaa atcatagacc
       61 atctggtcgc tccatccgtg ataagcacct cccattttta tgattttctt tttacctgtt
      121 tttatacgtg ccaaacgcaa agctcccatg acagcctcag tgccactgcc caacatacga
      181 aactgctcaa cagatggaat atgtttgcaa atcagttcgg caagtttata ttcatattca
      241 tgcagcaaac ctgttgatgg accacattca ttaattaatt caatcgcttt taatcttacg
      301 gtttcataat tactgcctaa gactgttggt cctccggctt gtaaaaaatc gatatattta
      361 ttgccatctg cgtcgtacaa atatgcccct tcggctttgg taaaagtaag cggaaacggg
      421 aaattgaaag ctaaattatg ctgcacaccg cccggaataa attgtctggc ttgctcatat
      481 atctcatgtg attttgtgca ttttgtttta taataatcca gatagttttg catggcatca
      541 gcttgaatat gctgcaaagg caaggcaact aatttgtcca atttctgata aatgggacga
      601 atatctgggt attcgttgat tgaaaatccg gttttatttt cgggagctga tattttggct
      661 gtcataacgt aatttttctc ctcttaaaat tactacattt gcagtattgc attttttgat
      721 aaatttcaat taatcttgaa tacacgacca cacagtttca cagactattt ggcaaaattt
      781 gggaatttaa gccctgaact tatccaatct gttgacaaga aaataaaagt ttcggtattt
      841 gccaagaaga caatactact gaaagccgga aaagtatgcg aatatcttta ttttattgaa
      901 aaaggaatgg cgcgaaatat tagtgaagaa gatgatagag tttttactaa tgacattgta
      961 ctggaaggag aaatactggt ttcatttgca agttttgtca gtcaaaaacc atcagtagaa
     1021 agcatcgaat tactggaaga cagtacatta tcctgtttgc attacgatga tttacaagat
     1081 ttatatactg actttcctga attggaaaaa attggcagaa agattgccga atatcattat
     1141 aattcgttgg cgataaaaac gcaccagtta cggtttttca gcagtacgca aagatatcag
     1201 catttatttg acaccaaaat tgaaatcata aaaagagcgc cactgggcat tattgcttct
     1261 tatttaggta tgacgattga gaatttaagc agaataagaa ataagtagtc atagtattgg
     1321 tattgaactt tcattggata aatattgttt taagtttgta ctgatttttc tgcgtatttt
     1381 cgcaacagtt attcaattca ttttatgcac gagctactac agcacattat tgataatcca
     1441 cttgcttcga ttactatcat cttaaatctc atcttaatag aaagtttgct ctctgtagat
     1501 aatgctgctg tcttagctac tatggtgatg gatttaccta aggaacaacg aggcagagca
     1561 cttaaatatg gcatcatcgg ggcgtatgtt ttcagaggga tttgtttatt atttgcagct
     1621 ctgctgataa aaatatggtg gttaaaacca ttgggtgggc tatacctgtt atatctggtt
     1681 tggaattggt ggagaggtaa acaaacaact actgtagaag atgatattgt agataaaaag
     1741 agtaattggt tttacagaaa tacggtgggc agactcggta tcttttggtc aactgttgta
     1801 ttagtagaaa ttatggactt agcattttct attgataatg tatttgcggc agtagctttt
     1861 acggataata ttcttttagt ttggacgggt gttttcatcg gcattatggc aatgcgccta
     1921 gtagctcagg gatttgtcaa cctgatggaa aagtatcctt ttttagaatc ttgtgctttt
     1981 ctggtcattg caattttagg tataaaatta cttttgtctg tttttgagca tcttcagccc
     2041 gagagtgctt tcagtaaatt tctgatgagt catgaagccg actggataac atcactgctt
     2101 actgccggta tcttcttcat tccggtttta acatcatact tatttaattt cccaaaaaaa
     2161 cacgaacaca aagaccaaga ttaatcttgt aacccgagtt cgggttaaga aaaataaact
     2221 gataaaatag ttgatttctc atttttttgt ccactttaaa aaggtggagg aattaaaagg
     2281 aggtggttct atataaaaaa tcaactattt ttttattgaa atgcttatcc cgaactcagg
     2341 attgtagttc ttcccaattt acaggctgta tttgccgctt atgacaaact atatttgccg
     2401 ctttatttgc taaggctgca attttttccc attcatagcc agatgcataa gccagcgcaa
     2461 ttactgcaat tactgtatct ccggcaccac atacatcggg atgtgcaata tatttatagg
     2521 gttctgtaaa aaatgaatct tgttcactta aacaataagt tcctctgcta ccgagtgtaa
     2581 ccaaaaccgt tttacagttt aattgatttc ttataaactc cgcacatcga acaatatctt
     2641 catcactttc cggttctgtt tccagctgat tcataaacgc cagttcttta cggttgggct
     2701 tgaatacatc aacggactga tataacatcc agttttcaaa tttaggatca acacaaaccg
     2761 ggattttatt ttctcgggca agctctaaaa acaaaggaat aagatatgga ttaagtactc
     2821 ccttattata atcctgaaaa attatgacat caggtttttg cttagtgatt atttctttga
     2881 aattaaacaa taaataatca tctattgctt tatcaaaatc tgcagttgtt tcttcatcta
     2941 ttctcagcag cggaattcca ttttgaagaa cccgggtttt gagtgtggta tgttgcttgg
     3001 gattagccac aacatactcc gtggaaatat tattactccg taataattca tgcaaaattt
     3061 cctgaaattt atctactccg gttgtaccaa gcaaaaaaac atctgcttct aattttgtgc
     3121 aattcaatgc tacattggca gcaccgccca aataataatt gcggtgttta acctgcaaaa
     3181 tgggattatt cgcatattcc tgagaaaaac cactttgctg agtttctaca aattcatcca
     3241 acataatatc tccgacaact aaaacttttt tttctttaaa cataatataa caacaattaa
     3301 agtatttgta ttttacttat ttaaaacaaa ctcactattc gaatccttct ttgcggtgaa
     3361 cgattaaatc ctcctgatga tgaatttgca aaggtgtcaa ttctatttct gtatttacag
     3421 tatccagccc gaattctttg tagggtggat agctgatttt agagagcaat tcataaatgt
     3481 tcatcaccaa taaattattt ggcttcaagt cattatcctc agaaagtttg ggtttgataa
     3541 agacgaattt aaaatcggct ttaatattat tttgccgcat ggaaggatat ctgcttaatt
     3601 catccacttc gcccaattct accatgtgtt ccactatttt gtgaaacagc ctgttgactt
     3661 tatgctttac tttaaaacca aattccagac ggacaaaaaa tacttttccc ggaataattg
     3721 ttttaacata ataatttttt tcatgttcat gcggatagtc taatgtatct acatgtataa
     3781 accaatacac atccgctctt tttgggtttt gctgaaaaat ggaataaata atattagaat
     3841 ctacattaga cgtgcgtttt gattttttag acatatagac caaattcgta gctgtctttg
     3901 gaatggtagt atcctgaatt aaatcggaaa acataccctt aaaatcatcc agattaacca
     3961 tgttattgta ggtatctctg atagcacgag ccttgtgcaa acaaaacaga atgaaaaaag
     4021 taactgaggc gataaaaaag gtaaaccaac ctccgtaaaa gaatttttta aggttggaca
     4081 tcaaaaacac ggattcaatg ctaaaaaaca atacaccaac agggataata aatgccaagg
     4141 acttattttt tatcttgaaa aacaacaaga gcaaggaagt cgtcatcagc atatcgatgg
     4201 taatcgtcaa accataagct gcttccatat ttgcagagcg tttgaaaagg atgatgataa
     4261 aaatacatcc gctcatcagc acccagttca taaccggtat atatacttgc cctttagaat
     4321 ctgagggata gtcaactttc agatttatcc aaagtcgttg tttgatggct tcactcacca
     4381 aagtaaaaca accactaatc aacgcctgac tcgcaataat tgtagccaca gtggccagta
     4441 taattaaaaa tgtaagatag ggtttgggaa tgagttcata aaacggcgaa acatcattta
     4501 ggctaattcc tctgtgttgt aagcaccaaa tagattgccc catatagttc agcagtaaac
     4561 aagccagcac atatatccaa ctgatttgaa tattgcgttt gccgcaatgt cccatatcag
     4621 cataaagggc ttctgctccg gtagtacaca gaaataccgc acctaagatc cagaaacctc
     4681 caggatgatt aaacaacaaa tcaaaggcat aaacgggatt aagggcttta aatatttccg
     4741 ggtgatgtat gacctttaat aaccccaaaa cgcctagcgt agtgaaccac agcaacatga
     4801 tgggtccaaa cgttttgcca atagtacccg taccgaattg ctgaaaaata aacaacagaa
     4861 acaaaatagt gagtaccacg ggaatagtag gcaaatccgg atataagata ttcatccctt
     4921 ctactgcgga tgatatagaa ataggcggag tgataaatcc atcggctaag agtgcagaac
     4981 agccgattaa agctgctatt aatggccatt tagcctgatg tttttttacc agtgcataca
     5041 aagaaaaaat accaccttcg ccctggttat ccgcttgcat gacgagtatc acgtatttga
     5101 atgttgtaac aatgataagc gtccagaata tgcaggacaa tgcgccaaaa atgatatccg
     5161 gctgtatggg tttatttccg ataatggctt tcatcacata caatggagat gtcccgatat
     5221 cgccataaat gatacccaat gtaatcagca aacctgatac acttagctta tttctcaaat
     5281 gattgtctgt actcaagttt caaagatagt taagaaaatt atagttttaa atgattatgg
     5341 tcattgaaat gaatatcgtc gtagtataat actagttctg cctgcctttt tagatatttc
     5401 attgaaaaaa tacaaatgaa acaacaaaca taactctgat gattacagcg caagcctggt
     5461 atttattaat tacaaacatc aagcatttaa tttttatttt ttcctttatt tcaaatcact
     5521 cttgcattta tacatgatgt gatttccttt ggtaaatcac agaatttcaa gataaggatt
     5581 actacgtttt acatcataat aaataaaaac aattacaata ttcttattaa tttcgtatcc
     5641 gttaattcct acatgcaaat cagaatagaa aataacatta aaagaagcgt actattttgt
     5701 gttggcttat tttgcatgtt ttttgtttta tcattcttag cattttataa aggctggaat
     5761 accggatgga attatgacag tatcgggttt tcatatctgg cgaaaaaaaa ttcgctctct
     5821 actttattaa gtttgcatat agacaaaaca tcccggtttg tttatcatac tgtttattat
     5881 ttactgaata agtattttgg ctatcaggga tgggactggt ttttatttaa aatagcctta
     5941 catgcattca atgcattact cttgttaata gtagtaaaat tgatactaca atattatgca
     6001 tcaataaaca actatgcgat accgattcta acagccttat tatttttatt agcacccaca
     6061 acaaccgaag ttgttttata tcaggctgcc atacaatttt tatttactta ttccctgctt
     6121 tacttatgca tactacttgt aatattctct atacgttcag gcaaaaaggc attcgtatgg
     6181 atttattttg ccttgttttt tatcgcatta ttttcgttag aaacttctta tacctttcct
     6241 gcaatagcat ttttgttagt tgcattttat cccggtataa aaatcaaatc taaacaaact
     6301 gttttagtag tactgatacc gatattgttt gttttaagtg tactatttat aaattacgca
     6361 ttatttggag attcacttag tcattatggg cagcaaaaac atttgaatat cagtttagat
     6421 aaacttatac agaacatgct gaaaattcca ttagatttta ttgcatttat agagtactgg
     6481 aaaatggatt acatttataa agtttataat attctatata agaaagaagc catttatctg
     6541 tttgctgttt tattcatttt gatttatctc agtttatgga aaaaatatct gaatgaccaa
     6601 ctttccaagc cattttggat gtgtattcat atcctttttg tcatgatatt gcctactatt
     6661 gcgctgggca tcctgtatat cataccgata gaagaagatc gttatttgta ttatccgagt
     6721 ggcatgattt tcttttgtat cgtttggata ttgtataaaa tctcgccgaa attgatgtat
     6781 gcaggcgttg catgcttatt atttattgga tttctatttc tgcaaaaaaa tatctactcc
     6841 tggaccatca ctcaaaaaac gattgactat agtttgtcat cttttcctta tgaaaagggc
     6901 aaaaggtata tactattggc aagtccggtt tatgcaaatg gcgtattaat ttgggatacc
     6961 catgaaacag acaaggatcc gtttgaagat aagtatcaga ttttgggaaa agcactttat
     7021 gtaaaacaaa acatagatgt gatggataaa atagcccccg ttttgcaata taatatcatc
     7081 aatgaaaaag aatcgccgga gatcagcact ttaaatgaca gtacgttgaa gatgacattg
     7141 ccctgttgtg gcaaatggtg gtggaaacac aatagcggtg cgcaggacat ggaaacaggc
     7201 tattataaaa taaaaatggt aggcggatgg caacaagaag tgcatattca atttaaacaa
     7261 aaaccaaaag atttggttta tatttatcaa tgtggaactc aattctgcaa gtatgatttt
     7321 tgagaactaa atattactct taacccattg tatagtttaa accacataga agcatagcat
     7381 ttttaatctt agtagttgct cgtacaaatc agtttagata gacaagtaaa aacatagttt
     7441 cgactttggc gattcctgta tatatctatt cttacaaata acaacagcga cttataaaca
     7501 tgtatgcatt atatatatat atatataata caccaagtcc tttaaatttt ttaacaaaaa
     7561 aaagtgaaaa taatcagtaa aataaataac ttagctacgt ataaacggaa tattaacgac
     7621 ataaatgttc gt
//
LOCUS       DAOQII010000109         7614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5256_length_7614_cov_4.616087, whole genome shotgun sequence.
ACCESSION   DAOQII010000109 DAOQII010000000
VERSION     DAOQII010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5256_length_7614_cov_4.616087"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2161)
                     /locus_tag="PLF48_06535"
     CDS             complement(<1..2161)
                     /locus_tag="PLF48_06535"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF49017.1"
                     /translation="MNVVTKKTITLTVFFLCFLHAAFSTHIVGGVMNYKYLGNNNFRI
                     DLYVYRDDIRAQPGAVLDNPAIIRVWDGVRETTQNFALPFDSFVPIGQIDPCATIAIN
                     ERIDWTHYSGIINLPPNTSGYTIYYQRCCRNNSISNLTFNSRGNGAMDWGATYTISIP
                     AAVNGIYINNTSPQILNYPPVYICNNKPITYDHRAIDENGDVLRYSLCTPFSGAFPND
                     PTNSGTLEPPPFQNVIWQAPYSQANMLGGIPLSINPVTGLLSGTPNTVGQFVVGICID
                     EYRNGVRLTRTIRDFQFNVVDCGLQVISSFFAPQVQCNNFTINFQNQSTGANSYTWYF
                     GDGDSSNVQNPAHTYRDTGIYTVTLVAYGINGTCNASYSQQVSVQYQLINADFILQNN
                     ACLKQGDSLRFFDRSTDTLNIVSWNWTFSNGQTSTVKNPVLVYDGTATSISATLTVTS
                     SNGCSSTLTKTFDLLKKPDISLPDSVVTCAGAGVLLPLSVSPNSNTTYAWSPATGLNN
                     SAIQQPTATVNANTTYVVTVNTTQGNAACIQKDTIKVKVTTTSVNIIQDSLKVCRDSV
                     RLSVNGVSNVQWSVNSNFTPVLSTRNPLVLYQTQPIVKYYVRGLVNGCLATDSIIVVY
                     SDTVPFINLDNSQLSCTNMITLNAVIQNGQNVIWSTSNTMSPVIGTSPSITLTQTPRT
                     QYYFITSSYRFCTSKDSIKVTIQDTLPVIQLPDQIRVC"
     gene            complement(2272..2520)
                     /gene="rpmA"
                     /locus_tag="PLF48_06540"
     CDS             complement(2272..2520)
                     /gene="rpmA"
                     /locus_tag="PLF48_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNF49018.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIYGGQAVIAGNIIIRQRGTK
                     FHPGEGVGIGRDHTLFALTEGKVSFKKGYKDRTFVSVI"
     gene            complement(2551..2859)
                     /gene="rplU"
                     /locus_tag="PLF48_06545"
     CDS             complement(2551..2859)
                     /gene="rplU"
                     /locus_tag="PLF48_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNF49019.1"
                     /translation="MYAIVEIAGQQIKVSPNQEVFVNRLGGNAGDKLNFDKVLLLSGD
                     AGTTVGSPTVSGSAVSATIVEHLKADKVTVFKKKRRKGYRVKNGFRASLTKIKIDTIA
                     "
     gene            complement(3161..4408)
                     /locus_tag="PLF48_06550"
     CDS             complement(3161..4408)
                     /locus_tag="PLF48_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF49020.1"
                     /translation="MIATGHSVLTLIIIGFILLEFLLDFILDKLNDTSWDKPIPAELE
                     GLYDDTQYQKAQSYHRELNKLSTLTSILSTTLTVLFLWFKGFAYLQIYIEQLTINPIG
                     QALVFFGLFAIVSDIISLPFELYGTFVIEEKYGFNKMSLKTFVADKLKGYLLGAVIGA
                     ALLSLFVLFYQYAGRMFWVYAWILFTVFSLFFVFIYTSWIMPLFNKFTPLATGSLREK
                     IEAFSQKVSFPLTKIQVMDGSKRSSKANAFFSGFGKKKTIVLFDTLINEQTDDELLSV
                     LAHEVGHYKMKHIQQGMIISILQMGLMLFLLSLFINEPKLSEALGYSTDKPVFHLGLI
                     AFALLYSPFSTLTGIVMNYISRKNEFEADNYAKENIGTGEYLISGLKKLSVNNLSNLN
                     PHPAYVFVHFSHPPLLERVRNLM"
     gene            complement(4436..5152)
                     /gene="rnc"
                     /locus_tag="PLF48_06555"
     CDS             complement(4436..5152)
                     /gene="rnc"
                     /locus_tag="PLF48_06555"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNF49021.1"
                     /translation="MFKFFRKLFSKKAPTYRTYEFQEIYDKIGFEPINSEDEYIKALT
                     HSSFTKSYNERNERLEFLGDSVLNFVVAYSLYHSMPNKDEGMLSKARAHIVNRKNLNR
                     IGTEIGIKKYLRHKLTAKQIEEAPDIVGNAFEAFIGAFYNEYGLHETETLLAGLLFAD
                     FDAQNFHTQITDFKSYFLEWCQAKKKSPFFEHTSNRKAENLFEVNLFLDNTFIASAVG
                     KNKKEAEINACKLAIEQLNL"
     gene            complement(5204..6466)
                     /gene="fabF"
                     /locus_tag="PLF48_06560"
     CDS             complement(5204..6466)
                     /gene="fabF"
                     /locus_tag="PLF48_06560"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNF49022.1"
                     /translation="MSAKRVVITGMGCITPIGNNLQQFSQNIFSGKSGAAKITGFDTT
                     LFRTQFACTVKDFFPENFFDRKELKKLDRYAQFALMSADEAIRHSGILDFPDLNKKRV
                     GVIYTSGFGGVQTFQDEVTQYIQSGRNPKYFSPYFVPRTLLDLAGGNISIKYGFKGMN
                     FSVVSACASSAHALLIALQFIRSGLADAVVVGGSEAPVNEISIGAFNAMRALSERNES
                     PETASRPFDKNRDGFVMGEGGACMVIESLEHARHRGANILAELAGGAMNADAYHITAP
                     QPEGEMITEVMQQALEDAHLVPNDVDYLNAHGTSTPLGDVAELQAIEHVFGSHISSLQ
                     VSSTKSMTGHLLGAAGITEAITAVLAIQEQKVPPTINLNEQDESINPAFKLTPNIAVN
                     QKISAVLSNNFGFGGHNASLVLRKYIDI"
     gene            complement(6563..6796)
                     /locus_tag="PLF48_06565"
     CDS             complement(6563..6796)
                     /locus_tag="PLF48_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNF49023.1"
                     /translation="MSSIAERVQKIIVEKLGVEESEVTPEASFENDLKADSLETVELI
                     MEFEKEFNIIIPDEQADKIKTVGEAIAYLEANV"
     gene            7277..>7614
                     /locus_tag="PLF48_06570"
     CDS             7277..>7614
                     /locus_tag="PLF48_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49024.1"
                     /translation="MPTKFFTNQDDNSLLKKFDGVFTNIDSIRHFDALVGYFRTSGYF
                     KVRAFLDKIPRIRILVGINVDQLIKKYHDKGQFYLDNPEETRADFLDEIIKNIQEADY
                     DEVTEKGIIQ"
BASE COUNT         2378 a         1465 c         1345 g         2426 t
ORIGIN      
        1 gacaaactct tatttgatcc ggcagttgaa taacaggcaa tgtatcttgt atggttactt
       61 ttatactgtc tttactggta cagaaacgat aagaagatgt aataaaataa tactgggttc
      121 ttggcgtttg tgtcagtgtt atacttggtg atgttcctat tacaggagac atagtatttg
      181 atgttgacca tattacattt tgcccgtttt gtatcacggc atttaaagta atcatattcg
      241 tacaagagag ctggctgtta tccaaattaa taaatggaac agtatcgcta taaactacaa
      301 ttatactgtc cgttgccaaa catccgttca ccagtcctct cacataatat ttaacaatag
      361 gctgagtttg gtacaatacc aaaggatttc ttgtactcag tacgggagta aaatttgaat
      421 ttacagacca ctgcacatta cttaccccat ttacactaag acgaaccgaa tcgcggcata
      481 ctttcaaaga atcctgaata atattgacag atgtcgttgt aacttttact tttatggtgt
      541 ctttttgaat acaggcagca tttccttgcg ttgtatttac tgtaacaaca tacgtcgtat
      601 ttgcatttac cgtagctgtt ggttgttgta tggcagaatt atttaaacct gttgccggcg
      661 accaagcata agttgtgttg ctgtttgggg aaacggataa aggcaataat acacccgcac
      721 ctgcgcaagt aacaacactg tcgggtaaac taatatccgg ttttttaagc aaatcaaatg
      781 tcttagtaag ggtagatgaa cagccgtttg aagaagtgac cgtcaatgtt gccgaaatgg
      841 aagttgccgt gccatcataa actagtacag gatttttcac cgttgaagtt tgtccgttag
      901 aaaatgtcca gttccaactc acgatgttta acgtatccgt gcttctgtcg aaaaagcgca
      961 acgaatcacc ttgctttaaa caagcattat tttgtaaaat aaaatctgca ttgattagtt
     1021 gatattgtac agaaacttgc tgagaataag aggcattaca agtgccgttt atcccatatg
     1081 caaccagcgt aacggtatag attccggtat cgcggtaagt atgtgctgga ttttgtacgt
     1141 tagaagaatc accatcgcca aaataccagg tataagaatt tgcacctgta ctttggtttt
     1201 gaaaattaat agtgaaatta ttgcattgca cttgaggcgc aaaaaagctg ctgattactt
     1261 gtaagccgca atctacaaca ttaaactgga aatcgcggat agtacgtgtt aatcttacgc
     1321 cattgcggta ttcatctata caaattccaa caacgaattg cccgaccgta tttggtgtac
     1381 cggacaagag tcctgtaacc ggatttatac tcaatggaat accccctaac atattagctt
     1441 gagaataagg agcttgccaa attacattct gaaacggagg aggctccaac gttccggaat
     1501 tagttgggtc gtttggaaat gcaccggaaa aaggcgtaca taaactatac cttagtacat
     1561 ctccattctc atctattgca cgatgatcat aagtaatcgg tttattattg caaatatata
     1621 cgggaggata gttcaatatt tgtggggaag tattattgat ataaatccca tttacagcag
     1681 ccggaattga gatggtatag gttgcccccc aatccatggc accattgcct ctcgaattaa
     1741 atgtaagatt actgatactg ttatttctgc aacagcgttg gtaatatatt gtatagccac
     1801 ttgtattcgg aggtaaattg ataattccgc tgtaatgcgt ccagtctatt cgctcattaa
     1861 tggcaatagt ggcacatggg tcaatttgac ctataggtac aaaactgtca aaggggagtg
     1921 caaaattttg tgtagtttca cgtacaccat cccaaactct tattatagcc ggattatcta
     1981 gcactgcacc gggctgggct cgtatatcat ctctatatac gtacagatcg attctgaaat
     2041 tattattgcc caggtatttg taattcatta cgccacctac aatatgagta gaaaatgcag
     2101 cgtgtaaaaa gcaaagaaaa aaaacagtaa gagtaatagt ttttttggtt acaacattca
     2161 tattatccag ttttaatgca taaatttagt tataataaaa tgaatatcta ctattccgga
     2221 aataaaaaaa ccacccgtgc gggtggttct ctatgttcac gtttctttaa attaaattac
     2281 agatacaaac gtacgatctt tgtagccttt cttaaagctc acttttcctt cagtcaaagc
     2341 gaacaaagta tggtctctgc ctatacctac gccttctcca ggatgaaatt tagtaccacg
     2401 ctgacggatg attatatttc cggcaattac tgcctgtccg ccatatattt taacgccaag
     2461 acgtttgctt tctgattctc tgccgttttt ggaactaccg acccctttct tgtgtgccat
     2521 gagtttataa ttttaagttg ctttataatt ttatgcgatg gtatcaatct tgattttagt
     2581 taaagaagct ctgaaaccgt ttttaacacg gtagcctttt cttctttttt tcttgaacac
     2641 tgtcacttta tctgctttca aatgttctac gatagtagct gaaacagctg aaccgcttac
     2701 agttgggcta cccacggtag tacctgcatc gccgcttaac agcaatactt tatcaaaatt
     2761 caatttatcg ccggcatttc cgcctaaacg gtttacgaag acctcctgat tgggagaaac
     2821 tttaatttgt tgtccggcta tttctactat tgcatacatg tctgaaaaat ttcgagtggc
     2881 aaagatacaa acttaattct attttttaaa ggttttttgt tgatttatta ttaagtccgt
     2941 cgcatttcat attatccaaa catcatttaa cataacaaca agataatcaa atttctataa
     3001 gataaaaaaa agcaatactc tcctacaatg cttattaaat gtatcatttt ttattccgct
     3061 gttgtcataa atttgtcttg ttgatgcaaa tcaacgccat taaccttctt aaatatgtag
     3121 ttagtttagc ggagcattcg tgttccgcta atctttttta tcacattagg tttcttactc
     3181 tctccagcag tggcggatgc gagaaatgta cgaaaacata agccggatgc gggttgaggt
     3241 tggatagatt attgacagat aattttttca gcccggagat taaatactca cctgtaccaa
     3301 tgttttcttt ggcataatta tcggcttcaa attcattctt acgggaaata tagttcatta
     3361 ctattcccgt cagtgtacta aatggagagt acaataaggc aaatgcgatt aaacctaaat
     3421 gaaaaaccgg cttatccgtg ctgtagccta atgcttcact cagtttaggt tcattgataa
     3481 ataatgacaa taaaaataac attaaaccca tttgaagaat ggaaattatc atgccttgtt
     3541 gtatgtgttt cattttataa tgtccaactt cgtgcgccaa tacactcaat aattcgtcat
     3601 cggtttgctc attaatgagg gtatcaaaaa gcacgatagt tttcttttta ccaaaccctg
     3661 aaaaaaaagc attagcctta ctgcttcgct ttgacccatc catcacctga attttagtga
     3721 gcggaaaact tactttttgc gaaaaagctt caatcttttc tcgtaaactg cccgttgcta
     3781 atggcgtaaa tttattaaac aaaggcataa tccaactggt gtaaataaac acaaaaaaca
     3841 agctgaatac agtgaataaa atccaggcat aaacccagaa cattcttccg gcatattgat
     3901 aaaacaaaac aaataaactc agcaaggcag caccgataac tgcaccaagc aagtatcctt
     3961 tcagcttatc ggcaacaaag gtttttagtg acatcttatt gaacccatat ttttcttcaa
     4021 tcacgaatgt accgtataac tcgaaaggca acgaaataat atctgacacg attgcaaata
     4081 aaccaaagaa aacaagggct tgtcctatcg gatttatagt gagttgttca atatatattt
     4141 gcaaataagc aaaaccttta aaccacaaaa acaaaacagt tagtgtagta ctcaatatcg
     4201 aggtaagtgt actcagttta tttagttcgc gatgatagga ctgcgctttt tgatattgtg
     4261 tatcatcata caagccttcc agttctgccg gaattggttt atcccaactc gtatcattca
     4321 gtttatccag gataaaatca agtaaaaact ccagcaaaat aaagccgatg atgatgagcg
     4381 taagcacact atgtccggtt gcaatcatga tactgatttt aaaatttatt cgtacttata
     4441 aatttagttg ttcgatggct aatttgcatg cattgatttc tgcttctttt ttatttttcc
     4501 caacagcaga agcaataaaa gtattatcca aaaacaagtt gacttcaaac agattttctg
     4561 ctttccggtt agaagtatgt tcaaaaaaag gagatttctt ctttgcctga caccactcca
     4621 aaaaataact tttaaaatca gtaatctgcg tatggaagtt ttgtgcatca aaatctgcga
     4681 acagcaaacc agccagtaaa gtttctgttt cgtgcaagcc atattcatta taaaatgctc
     4741 cgataaaggc ctcaaaggca ttacctacta tatcgggagc ttcttcaatt tgctttgccg
     4801 tcagcttatg acgcaaatat ttttttattc caatttcagt gcctatcctg ttcaggtttt
     4861 tgcggtttac gatgtgcgca cgagctttag acaacatgcc ttcatcttta ttgggcatgg
     4921 agtgatacag agaatatgcc acaacaaaat tcaagacgga atcgcctaaa aattcaagcc
     4981 tttcatttcg ttcgttataa ctttttgtaa aagaagagtg ggtaagtgct ttaatatatt
     5041 cgtcttctga attgatgggc tcaaacccta tcttgtcata tatttcttga aattcgtacg
     5101 tcctgtaagt cggagctttt ttgctgaata atttcctgaa aaatttaaac atcgctgcaa
     5161 aggtattact tttttgcaga ttgaatgaca ataaattatg ctatcaaatg tcaatatatt
     5221 ttcttaaaac caacgatgca ttatgtccgc caaagccgaa attattagat aaaactgcac
     5281 tgattttctg attaactgca atatttggtg tcagtttgaa tgcaggattt atgctctcgt
     5341 cttgttcatt caagtttatc gtaggaggca ctttttgttc ttgtattgcc aatacggctg
     5401 taatggcttc tgtaattcca gctgctccca gcaaatgccc cgtcatcgac ttggtagaac
     5461 tgacctgtaa actggagata tgactgccaa agacatgttc tattgcttgc agctcagcca
     5521 catcacccaa gggtgtggat gttccatgtg catttaaata atctacatca ttgggtacta
     5581 aatgggcatc ttccagcgcc tgctgcatca cttcggttat catttcacct tccggttgcg
     5641 gtgccgtaat atgataggca tcggcattca tggcaccacc ggcaagctct gccaaaatat
     5701 ttgcaccccg gtgccgggca tgttcaagtg attcgatgac catgcatgct ccgccttcac
     5761 ccatcacaaa accgtctctg tttttgtcaa atgggcgcga agctgtttcc gggctttcgt
     5821 ttcgctcaga taatgcacgc atcgcattaa atgcacctat agatatttca tttaccggcg
     5881 cttcacttcc acccacgact acggcatctg ccagtccgga acgaataaac tgaagggcaa
     5941 tcaacagggc atgtgctgat gatgcacaag ccgatactac ggaaaaattc atacctttaa
     6001 atccgtattt tatggagata tttccgccag ctaaatccag caacgtacgc ggtacaaaat
     6061 acgggctaaa gtatttggga tttctgccac tttgaatgta ttgagttact tcatcctgaa
     6121 aagtctgaac gccgccaaag ccggatgtat atataacacc aactcttttt ttattcagat
     6181 caggaaagtc caagataccg gaatgccgaa tggcttcgtc tgcactcatc aaagcgaact
     6241 gcgcatagcg gtcgagcttt ttcagttctt ttcggtcaaa gaaattttca ggaaaaaaat
     6301 cttttaccgt acatgcaaat tgtgtacgaa agagtgtggt atcaaatccg gtaatttttg
     6361 ctgccccgct tttgccacta aagatatttt gagaaaattg ctgcaaatta tttcctatcg
     6421 gggtaatgca tcccataccg gtaatgacaa ctcgttttgc agacatgcaa ctaagataaa
     6481 aaaatatcgg attaaattcc ggttagtgga ataatattta tgctatacca agatataggc
     6541 aactcataag aaacaattgc gattacacat tggcttccag ataggctatc gcctctccta
     6601 ccgtttttat tttatcggcc tgttcgtcag gtatgatgat attgaattct ttttcaaatt
     6661 ccataatcag ttctacggtt tctaaggagt ctgctttaag gtcattttca aagctggctt
     6721 ccggagtaac ttcgctttct tcaacgccca atttttcaac gattattttt tgaacgcgct
     6781 cggcaataga tgacatattt atttttgttt tactaaggac agcaaaatta gaggaaattt
     6841 tagttttctg tataaaaaaa ataaaaaaat acacaaaata ttgattatta atcaattaga
     6901 cagtgtgata aatttataca aactaaaaat tttgagcttt agttctggaa taattatgtt
     6961 tttatattta gtagtatata attcatataa tatctatagt tatatttaat aatgtacgtg
     7021 aagttaatgt acattaatgc taaagctcat tcacttttac atatcataaa taaagtaatt
     7081 aatgtaattt attaaactat tttattacat cgtattaact gacagttgta catttgttat
     7141 gctcataata aaaagccact atcacaaaac taaaaagcaa aatctctcca tctctaagca
     7201 aatagtttat aaaaataagg tatatttgac aaaagatgta ttagctgtcg aaaaacaaat
     7261 taaaatagaa aaataaatgc caactaagtt ttttacaaat caagacgaca acagcttact
     7321 caaaaaattt gatggggtat tcacgaatat tgatagcatt cgtcattttg atgcactcgt
     7381 tggctatttc agaacttctg gttatttcaa agttcgagct tttctggaca agattcccag
     7441 aatcagaatt cttgtgggaa ttaatgttga ccaactcata aagaaatatc acgacaaagg
     7501 acagttttac ctagacaacc ctgaagagac cagagcagac ttcttggatg aaatcatcaa
     7561 aaacattcag gaagcagact atgatgaagt aactgaaaaa ggaatcattc aatt
//
LOCUS       DAOQII010000110         7593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5285_length_7593_cov_4.218228, whole genome shotgun sequence.
ACCESSION   DAOQII010000110 DAOQII010000000
VERSION     DAOQII010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5285_length_7593_cov_4.218228"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="PLF48_06575"
     CDS             complement(<1..1407)
                     /locus_tag="PLF48_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF49025.1"
                     /translation="MKKFGISLLFFLFCITIYGQSCSIKLSGSILDLHDKSSLDDANI
                     YLLESQQGASSDSLGNFIVENICPGNYHVIISHIGCKDDTIQMEISHTTVKNFYLEHH
                     WEELQEIVTTATYIKKTEENKLSKDDISKNSGKDLGNVLKSINGVNLIKSGNNINKPV
                     LHGLYGSRITIVRDNSRLESQDWGMEHAPEMNPYDAASIQVNDDLSGIRYAVTALNGN
                     IEISGNKYKYHKSLKADAAYALQSNGWKNTISAQLQQGFNMGVAYKISGLFAINGDAH
                     APNYNLTNTAGREGNVSAGISLFKKNWKATLEYAYFNQTIGILRSTHIGNITDLLNAI
                     QSDKPLIIEPFSFQINNPKQKVNHHTINAELVKIKNNSEWILRYNFQQDRRKEYDIRR
                     GGRSDIPALDMHLWSEQFNGIHKYAKTTQKNIAITLENGICAGYKDNTNNYETEIKPL
                     IPDYQLYNAGAYNLSTFTY"
     gene            complement(1459..1851)
                     /locus_tag="PLF48_06580"
     CDS             complement(1459..1851)
                     /locus_tag="PLF48_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNF49026.1"
                     /translation="MKFFVFSQILFLSVFNMHAKPTKKSHAPQYEMKQYFFVMLTKGT
                     NRTQDSVTAASIQKAHLENIDRLAKLGKIIVAGPFGDDSNWRGLFIFDCATKEEAEQL
                     LNTDMAVSSGRLAYEIHPWWTAKNCVFK"
     gene            complement(1908..2468)
                     /locus_tag="PLF48_06585"
     CDS             complement(1908..2468)
                     /locus_tag="PLF48_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49027.1"
                     /translation="MSICAIALSLLVSSCKKDKEADDPNIAHVAINKTITPTVGLSLN
                     DSLDLNGDNINDFNLLSYQNVAGDTVITHISTSNSLLYIDSTKVMSISAEIKPLNENE
                     TPSLVDGSYKWYYITYCGIKFGSATTLGIAGQGDKFIPVAIRVSLNTFKYGWLRINIS
                     SDCKTFKVIDGAYSLLDNTPIKMGAE"
     gene            complement(2517..3101)
                     /locus_tag="PLF48_06590"
     CDS             complement(2517..3101)
                     /locus_tag="PLF48_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49028.1"
                     /translation="MKKIKTFARLIIACIAIFFSVACKKEKKADDPNISYREINQTIV
                     ANTNFSIYDSIDLNQDGHQDYYIQHIKNVAGDSVYCLIMSDNAGVYVDTTNAYSSYYV
                     IKSLDTDVSPVKYNYVAHIWWPTAIIGYKMNTTVNGIGGQGNKYLPVAMVKNDSTLNY
                     GWLQINLSSDNKTLKIIDGAYSTLDNTPIKMGAE"
     gene            3261..4214
                     /locus_tag="PLF48_06595"
     CDS             3261..4214
                     /locus_tag="PLF48_06595"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HNF49029.1"
                     /translation="MQNFLSVNDVQDVEQLAKDAITLKSNPYAHKYLGENKTLVLLFM
                     NASMRTRISTQKAAQNLGMNVIVLNVGQDSWNMEFEDGAIMNGNSVEHIKEGAAVISK
                     YADIIGLRSFPTLTDKAKDYSEFVLHKFLENIDVPLLNMESATVHPLQSLADLITIRE
                     NTTKPKPKIVLSWAPHIKPLPQCVSNSFAEWMNQADVELIITHPKGYELNEKFTQNAV
                     IEYNQVRAFENADFIYAKNWSSYSDYGKILDNHNDWIIDAKKMQLTNDARFMHCLPTR
                     RNVEVADAVLDSPNSLVVQQASNRVWSAQIVLKKMLESVSI"
     gene            complement(4221..5771)
                     /locus_tag="PLF48_06600"
     CDS             complement(4221..5771)
                     /locus_tag="PLF48_06600"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNF49030.1"
                     /translation="MTHEEKKQLLQQKNADALLGGGKNRIEAQHKKGKLTARERIELL
                     VDKNSFQELGKLVTHRATDFGIDKENYLGDGVVTGFGTINGRLVYIFSQDFTVFGGSL
                     SETHAEKICKIMDLAAKVGAPLIGLNDSGGARIQEGVVSLGGYADIFHRNVQFSGVIP
                     QLSAIMGPCAGGAVYSPAMTDFTLMVENTSYMFVTGPNVVKTVTHEEVSSEELGGAMT
                     HASKSGVTHFTAKNEVECIEQIKKLLSYLPQNCEDRAPNYPYQSEQPEIREKLINILP
                     QNPNQPYDMRDVIEAVSDENSFLEVHKNFAENIVVGFARIAGRSIGIVANQPAYLAGV
                     LDINSSRKGARFTRFCDAFNIPLLVFVDVPGFLPGTDQEWNGIIVNGAKLLYALSEAT
                     VPKVTVITRKAYGGAYDVMNSKHIGADMNYAWPTAEIAVMGPKGAAEIIFKHEIAKAE
                     NPEQKLQEKIDEYTDTFANPYKAAARGFIDEVILPEETRLKLIHAFSMLENKVVQSPK
                     KKHGNIPL"
     gene            5958..7406
                     /gene="miaB"
                     /locus_tag="PLF48_06605"
     CDS             5958..7406
                     /gene="miaB"
                     /locus_tag="PLF48_06605"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNF49031.1"
                     /translation="MSNELLDYIENKKHDEARQGEALDVSLQETELRNNKHFYIESYG
                     CQMNFSDSEIVASILANQGYSLTTNIFDADLVLLNTCSIREKAEQTVRNRLNSINDLK
                     SQKPGLLIGILGCMAERLKSKLLEEEHLVDLVVGPDAYRSLPELLAEAENGQKAVNVL
                     LSREETYADISPVRLDKNGVTAFISIMRGCDNMCSFCVVPFTRGRERSRNPESIVKEA
                     RDLFEKGYREVTLLGQNVDSYKWKGESNNENKETEIITNFAQLLEKTASVHPDLRVRF
                     STSHPKDITDEVLETMAKHENICKYIHLPVQSGNTACLERMNRTYTREWYLDRVDAIK
                     RIIPGCGLSSDMIAGFCGETEEEHEDTLSLMDYVKFDMSYMFFYSERPGTLAARKFKD
                     DVDLETKKRRLNEIINLQNKHSIENNQQDVGKVFKVLIEKTSKKSEDEFSGRTSQNKM
                     VVFPREHYKIGEYVNVLIEKATGATLIGKVVS"
BASE COUNT         2394 a         1389 c         1338 g         2472 t
ORIGIN      
        1 atacgtaaat gttgacaaat tataagcacc tgcgttatac aattgatagt ctggaataag
       61 tggttttatt tcggtttcat agttgttcgt attgtcttta tatcccgcac aaattccatt
      121 ttctaatgtg atagcaatat ttttttgtgt ggttttagca tatttatgaa tgccgttaaa
      181 ttgctccgac cacaagtgca tatccagtgc cggaatatca cttcttccac ctcgtcgtat
      241 atcatattct ttgcgtctgt cttgttggaa attataacgc aaaatccatt cactgttgtt
      301 ttttattttt accagttcag catttatggt atgatgattt accttttgtt tcgggttatt
      361 tatttgaaat gaaaatggtt caataatcaa aggtttatcc gattggatgg cattgagcaa
      421 atctgtaata tttccgatat gtgtactgcg caagatgccg atggtttgat tgaaataagc
      481 atattccaat gtggctttcc agtttttctt aaataaactg attcctgcac ttacatttcc
      541 ttctctgcct gccgtattcg tcaggttata gttgggtgca tgtgcatcgc cattgatggc
      601 aaataaaccg gaaattttat atgcaactcc catgttaaaa ccttgctgca actgggcaga
      661 aatggtattt ttccatccat tcgattgtaa agcataagcg gcatctgctt tcaggctttt
      721 gtggtattta tatttattac ccgaaatttc gatatttcca ttcaaagcag taacagcgta
      781 gcgaataccc gataaatcat catttacttg tatggatgcg gcatcatacg gattcatttc
      841 cggcgcatgt tccattcccc aatcttgcga ttctaatcga ctattgtctc ttactatcgt
      901 aatccggcta ccatacaaac catgcaaaac cggcttatta atattattgc ctgattttat
      961 caaattcacg ccatttatcg attttagtac attgcccaaa tcctttccac tgttttttga
     1021 aatatcatct ttagataatt tattttcttc ggttttttta atataagttg ctgtcgttac
     1081 aatttcctgt aactcttccc aatgatgttc caaataaaaa ttctttactg ttgtatgact
     1141 aatttccatt tggatggtat cgtctttaca gccgatatga ctgatgatga cgtgatagtt
     1201 gccggggcaa atattttcta caataaaatt tcccagtgaa tctgatgatg cgccttgttg
     1261 gctttcgagc aaataaatat tagcatcatc taaactcgac ttatcatgca agtctaaaat
     1321 gctgccgcta agtttaatcg aacacgattg gccgtaaatt gtaatgcaga ataaaaaaaa
     1381 caaaaggcta atgccaaatt tcttcatgca acaaagttac ataaaatctc gaaaatgcaa
     1441 tactgttgca gttaaaattt atttaaaaac acagtttttg gctgtccacc aaggatgtat
     1501 ttcataagcc aatctaccag atgaaactgc catatccgtg ttcagtaatt gctctgcttc
     1561 ttcttttgtc gcacagtcga aaataaatag cccacgccaa ttgctgtcat caccaaatgg
     1621 tccggctaca ataattttac ccaattttgc cagtcgatct atattttcta aatgagcttt
     1681 ttgtatggaa gctgcagtaa ctgaatcctg agttcggtta gtacctttgg taagcatcac
     1741 gaaaaaatat tgtttcattt catattgagg tgcatgactt ttcttggtag gttttgcatg
     1801 catattgaag acagataaaa acaaaatctg gctaaaaaca aaaaacttca tactcaaact
     1861 tttaagcatg aagttagtaa taaaataaac aaataacgga atgtatttta ttctgcgccc
     1921 atttttattg gcgtattatc cagtaaacta taagccccat caataacttt gaacgtctta
     1981 caatctgaac ttatatttat tcgcaaccat ccatatttaa aagtatttaa actaacacga
     2041 atagctactg gtataaactt atctccttga cctgcaatac ccaacgtagt ggctgaaccg
     2101 aatttaatgc cacaataagt aatgtaatac cacttgtaac ttccatctac caatgacgga
     2161 gtttcatttt catttaatgg tttaatttca gctgaaatac tcatgacttt tgtagaatct
     2221 atataaagta agctgttgga tgtactgata tgagtgatta ctgtatcacc ggctacattt
     2281 tgataagaca gcagattaaa gtcattgata ttatctccgt ttaagtccag actgtcatta
     2341 agtgatagac caactgtagg agttattgtt ttattaatag caacgtgagc gatgttaggg
     2401 tcatctgctt ccttatcttt tttacaagaa gaaactagca ggctaagtgc aattgcacaa
     2461 atagacaaag aaataaattt tgttttcata atattatttt gattattaat aataatttat
     2521 tctgcaccca tttttattgg tgtgttatcc aatgtactgt atgcaccgtc aatgattttc
     2581 agtgttttat tgtcagaact taggtttatt tgcagccaac cataatttag cgtagaatca
     2641 ttcttgacca tagccaccgg aagatattta tttccttgcc ctccaattcc attgaccgta
     2701 gtattcatct tatatccaat tattgcagtt ggccaccaaa tatgtgcaac atagttatat
     2761 tttactggcg atacatctgt atccagactc tttattacat aataggaaga atatgcatta
     2821 gttgtatcaa cgtacactcc ggcattatca gacattatca gacaataaac agaatctccg
     2881 gctacgtttt taatatgctg aatataataa tcttgatgtc cgtcttggtt taaatctatt
     2941 gaatcataaa tagagaaatt agtatttgcc acaattgttt ggtttatttc acgataactg
     3001 atattgggat cgtctgcttt cttttctttt ttacaagcta cggaaaagaa aatggcaatg
     3061 catgcaatta ttaagcgtgc gaatgttttg atttttttca tagtatttta ttttttactt
     3121 caaatttata ctgctttttt tatttactaa aaataaatga gaatttggca gttcttattg
     3181 agaattcgta aaaatcttag tgattttttg acatgaagtc gttgtataat tttataattt
     3241 cattcacaaa ttaataagta atgcaaaatt tcttatccgt aaatgatgta caggatgtag
     3301 agcaattggc taaagatgca ataactctaa aatcgaatcc ttatgctcat aaatatttag
     3361 gcgaaaataa gacgctggta ttactcttta tgaatgcatc aatgcgcacc agaatttcta
     3421 cacaaaaagc agcacagaat ttgggtatga acgtgattgt attaaacgtg ggtcaggatt
     3481 catggaatat ggagtttgaa gatggtgcga taatgaatgg aaattctgta gaacatatta
     3541 aggaaggtgc agctgtcatt tcaaaatacg cagatatcat aggattgcgc tcctttccta
     3601 cattaaccga taaagcaaaa gactactcgg aatttgtttt gcataaattt ttggaaaata
     3661 tcgatgtccc cttgttgaat atggaaagtg ctactgtgca tcccttgcaa tcattagccg
     3721 acttaattac cattcgagaa aatacgacaa aaccgaaacc gaagattgta ttgtcgtggg
     3781 cacctcatat caaacctttg ccgcaatgcg tatccaactc atttgccgaa tggatgaatc
     3841 aggcggatgt ggaattgatt attactcacc cgaaaggata tgaattaaat gaaaaattta
     3901 ctcaaaatgc agtaattgaa tataatcagg tacgagcatt tgagaatgct gattttattt
     3961 atgcaaaaaa ctggagttca tattcggatt acgggaaaat tttagacaat cataacgact
     4021 ggataattga tgcaaaaaaa atgcaattga ctaatgatgc ccgtttcatg cattgtttgc
     4081 caacacgacg taatgtagag gtagcggatg cggttttaga ttcaccaaac tcacttgtcg
     4141 ttcagcaagc aagcaatcga gtttggagtg cgcaaattgt attaaagaaa atgttggaaa
     4201 gtgtttctat ataaattaac ttataaagga atattcccgt gtttcttttt aggagattga
     4261 actactttgt tttccagcat ggaaaatgca tgaatcaatt taagtctcgt ttcttcgggt
     4321 agaattactt catcaataaa accgcgggct gcagctttat atggattagc aaaggtgtcg
     4381 gtatattcgt ctattttttc ctgtaatttt tgctccggat tttccgcctt tgcaatttca
     4441 tgtttgaaaa taatttctgc cgcacccttt ggtcccatta ctgcaatttc tgccgtgggc
     4501 caggcatagt tcatatctgc accaatatgc ttggagttca ttacatcata agctccacca
     4561 tatgcctttc ttgtaataac tgtaactttc ggaactgttg cttcacttaa tgcatataac
     4621 aatttcgccc catttacaat gatgccattc cattcctgat cagtaccggg taaaaaaccg
     4681 ggaacgtcaa caaaaaccaa taaagggatg ttgaaagcat cgcaaaaacg ggtaaatcgt
     4741 gctcctttcc tgctgctgtt gatatcgagc actccggcta aataagctgg ctgattggct
     4801 acaatgccaa tgcttcttcc tgcaatgcgc gcgaagccca ccacgatatt ttctgcaaaa
     4861 tttttatgca cttcaagaaa tgaattttca tcagaaacag cctctattac atcacgcata
     4921 tcataaggct gattgggatt ttgaggtaaa atgtttatta atttttcccg aatttctggt
     4981 tgttcagact gataaggata attaggcgca cggtcttcac aattttgtgg caaatagctc
     5041 aacaactttt taatttgttc gatacattct acttcatttt ttgccgtgaa atgcgtaaca
     5101 ccgctttttg aagcgtgcgt cattgctcct cccaactctt cagaacttac ttcttcgtgc
     5161 gtaacggttt ttacaacgtt cggacctgta acaaacatgt aggatgtatt ttctaccatc
     5221 aacgtgaaat ctgtcatggc cggggaataa actgctcctc ctgcacaagg ccccatgatt
     5281 gctgaaagtt gcgggatgac accggaaaat tgcacattgc gatgaaatat gtcagcatat
     5341 cctcctaatg atactacgcc ttcctgaata cgggcaccac cagaatcatt taatccaatt
     5401 aagggcgcac ctactttggc agccaaatcc atgattttgc aaattttttc agcatgtgtt
     5461 tcagataaag aaccaccgaa aacagtaaaa tcctgggaga aaatataaac caatctgcca
     5521 tttatagtgc caaaacccgt tactacacca tcccctaaat aattttcttt atcaatacca
     5581 aaatctgtag ctctatgcgt taccaactta cccaactctt gaaacgaatt tttatcaact
     5641 aataattcta ttcgttcacg ggcagtcaat ttgccttttt tatgttgagc ctctatcctg
     5701 ttttttccac caccaagtaa cgcgtctgcg tttttttgtt gaagtaattg ctttttttct
     5761 tcgtgcgtca tatctgcaaa aatactgaaa atagcggatt ttaactaaat gccaaattgg
     5821 cagtaactga ttttttgtat ctttgcgaaa gagagaagag ttaaagagtt tacaagttaa
     5881 ataatttaat attttataaa taaaaatata attcttcata actattaatg accaattaac
     5941 caataaccag actaccaatg tccaacgaac tgttagatta tatcgaaaac aaaaagcacg
     6001 acgaggcacg tcagggtgaa gctttggatg tgtcactgca agaaacggag ctcagaaaca
     6061 acaagcattt ttatattgaa agctacggct gtcaaatgaa cttttcagac agcgagattg
     6121 tagcttcaat tttagccaac caaggttatt cgcttacaac caatatcttt gatgctgatt
     6181 tggtattgct caatacgtgt tctatccgtg agaaagcaga acagacggtt agaaaccggc
     6241 ttaattccat taacgatctt aaatctcaaa agccgggttt actaatcggc attttaggct
     6301 gtatggcaga gcgcttgaaa tcaaaactac tggaagaaga acatttggtt gatctcgtgg
     6361 taggtccgga tgcttatcgc agtttgcccg aacttttagc cgaagccgaa aatgggcaaa
     6421 aagcagtaaa tgtattattg agcagggaag aaacatatgc ggatatctct ccggttcgct
     6481 tggacaaaaa cggggtaaca gcatttatat ctatcatgcg gggttgcgat aatatgtgtt
     6541 cgttttgtgt ggtgccattt acacgtggca gagagcgtag cagaaatcct gaatcaatag
     6601 taaaagaagc cagagattta tttgaaaaag gctatcgaga agttacctta cttggacaga
     6661 atgtagatag ttacaaatgg aaaggtgaaa gtaacaatga aaataaggaa actgaaatta
     6721 ttacaaattt tgctcagctt ttagaaaaaa cagcctccgt tcatcctgat ttgagagtac
     6781 gtttttctac ttcacatcca aaagacatta cagatgaagt tttagaaact atggcgaagc
     6841 atgaaaatat atgtaagtat atccatcttc cggtacaaag cgggaataca gcatgtctgg
     6901 agcgcatgaa ccgcacctat actcgtgaat ggtatttaga cagagtagat gcgataaaac
     6961 gcatcatccc gggttgtggc ttgtcttcag atatgattgc aggattttgc ggtgaaacgg
     7021 aagaagaaca cgaagacaca ctaagcctga tggactatgt aaaatttgat atgagctaca
     7081 tgttctttta ttcggaaaga cccggtactt tggcagctcg aaaatttaaa gatgatgtag
     7141 atttggaaac caaaaaacgt cgtttaaatg aaatcatcaa tttgcaaaac aaacattcta
     7201 tcgaaaacaa tcagcaagat gtaggaaaag tctttaaagt attgatagaa aaaacatcaa
     7261 agaaaagtga agatgaattt tcaggcagaa catcacagaa taaaatggtc gttttccctc
     7321 gtgaacacta taaaattgga gaatatgtaa atgtgctgat agaaaaagct acaggagcta
     7381 ctttgatagg aaaggtggtg agttaggtaa ttggtattaa tataatattt aatacactat
     7441 taaagaaaat acaaactaat tacttaacag ctcattttca aattacctca ttttcaaatt
     7501 atctaatttt caaattatct aattttcaaa ttatcctcgt ggaacttcaa tcaataaaac
     7561 aacgcttcgg catcattggc aactcgcctc ttt
//
LOCUS       DAOQII010000111         7589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5290_length_7589_cov_4.687815, whole genome shotgun sequence.
ACCESSION   DAOQII010000111 DAOQII010000000
VERSION     DAOQII010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5290_length_7589_cov_4.687815"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLF48_06610"
     CDS             <1..307
                     /locus_tag="PLF48_06610"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNF49032.1"
                     /translation="SECINESKGQWYYRQILGSCNFSGSRLMHIMSGHLSFQVEHHLF
                     PDIPSSRYEALSKEVQVICQKYNIPYNTASMSKQYVSVIKKMFRNSFPNGNKQLAKA"
     gene            complement(329..1276)
                     /gene="nusB"
                     /locus_tag="PLF48_06615"
     CDS             complement(329..1276)
                     /gene="nusB"
                     /locus_tag="PLF48_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNF49033.1"
                     /translation="MITRRSVRTKAMQYIYAFETAQTDDISAYEQNLLKSIQSVKEQY
                     LFVLFYLREVANYVETDAKIKSGKFIKTEKDKNFNTKLLSNILVQYLNNDTAFLNELK
                     KHKTSQLADEELIRDLYKSFISAPEYQDYLNLSTEFDLEEDKKIISFLLKDILLQNEA
                     FMQFAEDNFVNWYDDGELVLESVDEVIFKSKTDLKLHLNKEKLNEKFDELTKFGRDLF
                     RYTIEHKKEYWKLIEPKLKNWDADRLAVVDMILMRMALTEFFNFPSIPLKVSINEYLD
                     IAKEYSTPKSKDFINGVLDSLMRELKDSGKIQKTGRGLI"
     gene            1392..3212
                     /locus_tag="PLF48_06620"
     CDS             1392..3212
                     /locus_tag="PLF48_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF49034.1"
                     /translation="MKELASINRYIARYKGLFFGGILFVLLSNVFGLYTPVYIRYAVD
                     AVKENLTIYNTIHSLSLSALFESAFFKLVIYFSGIILITSLIKGLLMFFMRQTLIVMS
                     RKVEYDQKNDIYKHYQTLSTEFYKQNNTGDLMSRISEDVSRVRMYVGPAIMYIINLIA
                     LFSMVIYTMFSINAYLSFLVLLPLPVLAFSIYKVSDIINKKSERISIALSGLTTEAQE
                     VFSGIRVIQSFALNKQVEDEFYAKSLGYKEDNISLAKVESFFAPLMLFLIGLSTIIVV
                     YVGGMEVNKGNFTTGNIAEFIFYINMLTWPVASLGWCVSLIQRAIASQKRINQFMDSH
                     QQITETEKLPIHQIKHIAFKNVSFTYTNTQIEALRDVGFEVAHKEKIAIVGKTGSGKS
                     TLAELLLRTYDATSGEISINNLPIQNLDLKVYRSRLGYTPQDVFLFSDTIKNNILFGE
                     NDSSLFEKNEPGIESGVKHELESLTKITHVYHDIMDLPKKFETTIGERGVMLSGGQKQ
                     RISIARSLAGDADVIILDDCLSAVDAKTEKIILDNLNHSLADKTVFFITHRLFSIMHF
                     DKILVLDEGQIAEQGTHEELMQKKGVYYDLYQLQMLEGNN"
     gene            3304..3684
                     /locus_tag="PLF48_06625"
     CDS             3304..3684
                     /locus_tag="PLF48_06625"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNF49035.1"
                     /translation="MENEKNFDKNGVYSKKVRAGKRRTYFFDVRTTKGNDYYLTITES
                     KKRFNEETYDRHKIFIYKEDFMKFIQALEETVDHIKTELLPDYDFDAPQHEYNDYPEE
                     HHQNSDEEQKDSSEQKSEEAEKWD"
     gene            3759..4715
                     /locus_tag="PLF48_06630"
     CDS             3759..4715
                     /locus_tag="PLF48_06630"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNF49036.1"
                     /translation="MRYIYIFIIFSFSIFLFACEKEINLNLKDPQNAVVVEGHIENGI
                     PPYVMLTKNSAFFSNLNLNNIASYFISGADVKIITDGDTIQLQEYNGALLQALPDSVV
                     IPLAQQFGLNISSAADFPPIIIYTVSIADSLFTGEFGKQYDLLIQYDGKQITSTTTIP
                     QPVTFDSLWIEPHPNAAYADSFFQLYGRLREPAMQGNFYRYFTRADDEAWLVSDQSVF
                     DDAFINGLTFKIFIPKGHGFGAQAGTNFNTSGYWNISDSVCTVKLMSIDKPHYDFWRT
                     LEANRSSNGNPFGSFVYVKSNVNGALGVWGGYASTVSSLRVF"
     gene            complement(4824..6305)
                     /locus_tag="PLF48_06635"
     CDS             complement(4824..6305)
                     /locus_tag="PLF48_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNF49037.1"
                     /translation="MGKPTGFLEYTRELPKYKKPEIRINDYQEFYETFPEESLNHQAA
                     RCMDCGVPFCHSGCPLGNIIPEFNDAVFKKEWQMALDILLSTNNFPEFTGRICPAPCE
                     SACVLGINQPPVTIENIEKSIIEKAFELNLIKANPPEDRTGKSVAVVGSGPSGLACAA
                     QLNKAGHAVTVFERADRIGGLLRYGIPDFKLDKNIVQRRVDLMKEEGIVFRMNANVGV
                     NVTIDELNEFDAIVLCGGSTVARDLPIPGRAFKGIYPAMEFLSQQNKRVANIETKADH
                     RGEVYENGDIWATDKHVIVIGGGDTGSDCVGTSNRHGAKSVTQLELLSKPPAERDEST
                     PWPLWPMMLRTSSSHEEGCTREWSILTKAFIGDEKGNVTGLKIVEIEWKTVDGKPQFV
                     EKPETERILPCDLAFLAMGFLHPQYEGLLQQLDIEVDSRGNVKDNDYKTNKSKVFTAG
                     DMRRGQSLVVWAISEGRECARSVDIFLMGESSLEAKDMSSLQV"
     gene            complement(6426..>7589)
                     /locus_tag="PLF48_06640"
     CDS             complement(6426..>7589)
                     /locus_tag="PLF48_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNF49038.1"
                     /translation="ATASLVVMGCIMMRKCHLNTCPVGIATQNTLLRGRFTGDPQHVI
                     NFFTFVAQDLRTWMAKLGFRTVNEMVGQQNLLKTKDNITHWKYKHLDLSPVINQPAVG
                     NEVGLHKLMEQAHPKHDQILDLKLIKAAEKSLEEMLPTTAAYPIINTDRTVGAMLSYE
                     ISSKYKGDGLPEDTIHIKFTGSAGQSFGAFAAPGIKFELEGEANDYFGKGLSGGKLII
                     YPSAKATFKASENIIIGNVAFYGATSGSAYIRGMAGERFCVRNSGATAIVEGIGDHGC
                     EYMTGGLAIILGKTGRNFAAGMSGGMAYVYDIEKTFAKNVNMEMVELDPMEQEDLDKL
                     RNHLEKHLAYTNSTTAKDILDNWEITSGYFVKVMPSDYKLVLQEKKAKKELIN"
BASE COUNT         2321 a         1509 c         1304 g         2455 t
ORIGIN      
        1 gtcagaatgc atcaatgaat ccaaaggaca atggtattac agacaaattt tgggttcgtg
       61 taatttttca ggttcgagac tgatgcatat tatgagtggt catttaagtt tccaggtaga
      121 acatcattta tttcctgata taccttcttc gagatacgaa gctttgtcaa aagaagttca
      181 agttatttgt caaaagtata atatccctta caacacggca tccatgtcta aacaatatgt
      241 ttctgtgatt aaaaagatgt ttagaaattc ttttccaaac ggaaataagc aattggctaa
      301 agcttgaaaa aacagattgt tagaaatatc aaattaaacc tctgcctgtt ttttgaattt
      361 tgccgctgtc tttcagttcg cgcatgagtg aatctaatac gccattgata aaatctttgc
      421 ttttgggtgt agaatattct ttggcaatat cgaggtattc attgatgctg actttcaaag
      481 gaatacttgg aaaattaaaa aattctgtca aagccatgcg catcaaaatc atatccacta
      541 cggccaaacg atcggcatcc cagtttttta atttaggttc aatcagtttc cagtattctt
      601 ttttatgttc tatcgtatag cgaaagaggt ctcttccgaa ttttgtcaac tcgtcaaatt
      661 tttcgtttaa tttttccttg ttcaaatgaa gttttaaatc ggttttactt ttaaagataa
      721 cttcatcaac cgactcgagc accagttcgc catcatcgta ccagttcacg aaattatctt
      781 ctgcaaactg cataaatgcc tcgttttgca gtaaaatatc tttgagcaaa aaagatataa
      841 tttttttatc ttcctctaga tcaaactcag ttgacagatt caggtaatct tgatactctg
      901 gagcagaaat aaatgattta tacaaatccc tgattaattc ttcatctgca agttgacttg
      961 ttttgtgttt tttgagttca tttaaaaatg cagtatcatt attgagatat tgcaccaaaa
     1021 tattgctcag taatttcgta ttgaaatttt tatctttttc agttttaatg aatttccctg
     1081 attttatttt cgcatctgtt tccacatagt tggctacctc gcggagataa aacaaaacaa
     1141 acagatattg ttcctttact gactgaatac ttttcagcag gttttgttcg taagcactta
     1201 tatcatcggt ttgagccgtt tcaaatgcat aaatatattg catggctttg gtacgaacac
     1261 ttcttctggt tatcataaaa taaggaacat ttagtcggca aagataataa aattaagagt
     1321 ccaagctttt ttaagctata attaatattt atagtcagct cgttcggcta aagctgatta
     1381 tctttagaca ggtgaaagag cttgcttcca taaaccgata tattgcccga tacaaaggat
     1441 tgtttttcgg tggcatctta tttgtactgc tctccaatgt ctttggtttg tatactccgg
     1501 tatatatccg ctatgccgta gatgccgtaa aagaaaatct gacaatatac aatacgatac
     1561 attcactcag cttgtcagct ttgtttgaat ctgccttttt caaattagtg atttattttt
     1621 caggaatcat tttgattacc tcactgatta aaggcttgct gatgtttttc atgcggcaaa
     1681 cattaattgt catgtcgcgc aaagtagaat atgaccaaaa aaatgacatt tacaaacatt
     1741 accaaacgct aagcaccgag ttttacaaac aaaataacac aggagattta atgagccgca
     1801 tcagcgaaga tgtctcaaga gtacgcatgt atgtcgggcc ggcaataatg tatatcatca
     1861 atttaattgc cttattcagc atggtgattt ataccatgtt ttccataaat gcatatctga
     1921 gtttcctcgt attattaccg cttccggtat tggcattttc catttataaa gtaagtgata
     1981 ttatcaataa aaagagtgag cgcatttcta ttgctttgtc cggattgact accgaggccc
     2041 aagaagtttt ttcaggtata agagttattc agtcttttgc gttgaataaa caagtagagg
     2101 acgagtttta tgccaaaagc ttaggttata aagaagataa tatttcattg gcaaaagtgg
     2161 agtctttctt tgctccttta atgttgtttt tgattggatt aagtaccatt atcgtagttt
     2221 atgtaggagg catggaggtg aacaaaggca atttcacgac cggaaatatt gcagaattta
     2281 ttttttacat caacatgctc acttggcctg tagcctcctt aggctggtgt gtttcattga
     2341 tacaacgggc tattgcttcg caaaaaagaa ttaatcaatt tatggatagc catcagcaaa
     2401 ttacagaaac agaaaaactg cccatccatc aaataaaaca tattgcattt aaaaacgtat
     2461 catttacata taccaataca caaattgagg cattaaggga tgtgggtttt gaagtagccc
     2521 ataaagaaaa aattgctata gtgggcaaaa caggcagcgg aaaatctacc cttgccgaat
     2581 tattgttacg cacgtatgat gctacatcgg gagaaatatc tataaacaat ttacccatcc
     2641 aaaacttaga tttgaaagtc tatcgctcaa gattaggcta taccccacaa gatgtctttt
     2701 tattttctga cacgataaaa aacaatattt tatttggaga aaacgactcc tctctttttg
     2761 aaaaaaatga accaggtatt gagtcgggag taaagcatga actggaatcg ctcactaaaa
     2821 ttacgcacgt ctatcatgac attatggact tacctaagaa atttgaaact accataggcg
     2881 agcgaggtgt catgttatcg ggcgggcaaa aacagcgcat atctattgcc cgttcattgg
     2941 caggtgatgc agatgtcatt attttagatg attgcttgtc ggcggtagat gctaaaacag
     3001 aaaaaattat cctggataat ctcaatcatt cattagctga taaaacagtt ttttttatta
     3061 ctcatcgttt gttttccatt atgcattttg acaaaatcct agtactcgac gaagggcaaa
     3121 ttgctgagca gggaactcat gaagaattaa tgcaaaagaa aggtgtatat tatgacttat
     3181 atcaattaca aatgctagaa ggaaacaatt aagcagttat tcacactctt tggaattgtg
     3241 cattaaaaaa aaaggcattt tttttcaatt caattaattt tgaaacaatt aaaatagaat
     3301 aaagtggaga acgaaaaaaa ctttgacaaa aacggtgttt attctaaaaa agtgcgcgcc
     3361 gggaaaagaa gaacttactt ttttgatgta agaacaacaa aaggaaatga ctattatctg
     3421 actatcacag aaagcaaaaa aagattcaac gaagaaactt atgaccgtca caaaatcttt
     3481 atttacaaag aagattttat gaaatttata caggcactgg aagaaacagt tgatcatatc
     3541 aaaactgaat tattacctga ttatgatttt gatgctccac aacacgaata caatgactat
     3601 cccgaggaac atcatcaaaa ttcagatgaa gaacaaaaag attcatctga gcaaaaaagt
     3661 gaagaagctg aaaaatggga ttaattttaa ttaatttttt taatttttac atttagtaag
     3721 agtcaaatca ttattttagc taagctaaaa ttgagctctt gagatacatt tatatcttta
     3781 taattttttc tttcagtatt ttcctttttg cttgcgaaaa ggaaatcaat ttaaatttga
     3841 aagatccgca gaatgcagtc gtggtggaag gacatattga aaacggcata ccgccatatg
     3901 ttatgctgac gaaaaactct gcatttttca gcaacctgaa tctgaataat attgcctcct
     3961 attttatcag cggggcagat gtgaaaataa ttacagatgg cgatacgata caattgcaag
     4021 aatacaatgg agcccttttg caagccttac ccgattctgt tgtaattcca ttagctcagc
     4081 aattcgggtt aaatatttcc tcagctgctg actttcctcc aataattata tatacggttt
     4141 caatagcaga cagtttattt acgggagaat tcggcaagca atatgattta ctgattcaat
     4201 atgacggtaa gcaaattaca tcaaccacta ccataccaca gccggtaact ttcgattctt
     4261 tgtggataga gcctcatccg aatgctgcct atgctgattc cttttttcaa ttgtacgggc
     4321 gattgcgcga gccggctatg caaggaaatt tctaccgcta tttcaccaga gctgacgatg
     4381 aggcctggtt ggtcagcgac caatctgttt ttgacgatgc ttttataaac gggttaactt
     4441 ttaaaatttt cattcccaaa ggacatggat tcggagcaca agccggtacg aatttcaaca
     4501 cttccggcta ttggaatatt tccgattcgg tatgcacggt aaaattaatg agtatagata
     4561 aaccgcacta tgatttctgg cgcacactcg aagccaaccg aagcagcaat ggcaatccat
     4621 ttggcagttt tgtctatgta aaatctaatg taaacggtgc cttaggcgta tggggtggct
     4681 atgcttctac cgtgagcagt ttgagagtgt tttagtccga attatatcct aatccaaccg
     4741 caaaaaaaat cccggcattt ctgccgggaa aaaagtcgca aagaaaacag caaattccaa
     4801 acgacttgta tctttttact tgtttatact tgtaaagatg acatatcttt agcttccaaa
     4861 ctactttctc ccattaaaaa tatatccaca ctgcgtgcac actcccgccc ctcgctgatg
     4921 gcccaaacta ctaaactttg tcctctgcgc atatctccgg ctgtaaaaac tttactctta
     4981 ttagttttgt agtcattatc tttcacgttt cctctactat ccacctctat atcaagctgt
     5041 tgcaataatc cttcatattg tggatgtaaa aatcccatag ccagaaatgc caaatcgcaa
     5101 ggtaaaattc gctcagtttc cggtttttcc acaaactgag gcttgccgtc cactgttttc
     5161 cattcgattt ctacaatttt taatccggtt acattgcctt tttcatctcc aatgaaggct
     5221 tttgtcaaaa ttgaccattc acgggtacag ccttcctcgt gagagctgga cgtacgcaac
     5281 atcatcggcc aaagtggcca gggcgtactt tcatcacgct ctgcaggagg ttttgacagt
     5341 aattcgagct gtgttacaga ttttgctcca tgacgatttg atgtacccac acaatctgat
     5401 ccggtatcac cgccgccaat tacaatcaca tgcttatcgg ttgcccaaat atctccgttt
     5461 tcatatactt ctcccctgtg atcagctttt gtttcgatat ttgcaacacg tttgttttgc
     5521 tgagataaaa attccatagc cggataaatg cctttgaatg ctctgcccgg aataggtaaa
     5581 tcacgggcaa cagttgagcc tccgcaaagt acgattgcat caaattcgtt caattcatca
     5641 atggttacgt ttacaccgac atttgcattc atcctgaaaa caataccttc ttctttcatc
     5701 aaatctacac gacgctgcac gatattttta tccagcttaa aatcgggaat accatagcgc
     5761 aacaaaccac caatacggtc tgcacgttca aaaactgtaa ctgcatgtcc tgctttattc
     5821 aactgtgccg cacaagccaa acctgatgga ccggatccaa caacagccac actttttcct
     5881 gttctatctt ccggcgggtt agcctttatt aaattaagct cgaatgcttt ttctattatt
     5941 gatttttcaa tattctctat agttactggc ggttggttga tacccaaaac gcaagcagac
     6001 tcacacggag ccggacaaat tcttccggta aactccggga aattatttgt tgacaacaga
     6061 atgtccaagg ccatttgcca ttctttttta aaaacagcat cattaaattc aggaattata
     6121 ttaccaagcg gacagccgga atggcaaaat ggtactccgc aatccatgca gcgtgccgcc
     6181 tgatgattta aactttcctc cggaaaagtt tcataaaatt cctgataatc attaatacgg
     6241 atttccggtt ttttatattt cggaagttcg cgtgtgtatt ctaaaaatcc tgtaggctta
     6301 cccatatcta aatgttgaat gttaaatttt gaatgttaaa ttttgaatta tctatttcgt
     6361 tataacaagt taaaactgca tactttctac ttacaactta ttacttttca caatttacct
     6421 ttcatctaat taatcaattc tttttttgct tttttttcct gtaaaaccaa tttataatca
     6481 gatggcatta cttttacaaa atagccggag gtaatctccc aattatctaa aatatctttt
     6541 gcagttgtac tgttggtata tgcaagatgt ttttccaaat gattacgtag tttatccaaa
     6601 tcctcttgtt ccatggggtc gagttccacc atttccatgt tcacattttt tgcaaatgtt
     6661 ttttcgatat cataaacata agccataccg ccgctcatac cggctgcaaa atttctacct
     6721 gttttaccca aaataatcgc caatcctccg gtcatatatt cgcaaccatg atcgccgata
     6781 ccttcaacta ttgcagtagc tcccgaattg cgcacacaaa aacgctctcc tgccataccg
     6841 cggatatacg ccgatccact cgttgcacca taaaatgcca cattgccaat gatgatattt
     6901 tcactggctt taaaagtagc tttagctgat ggataaatga ttaatttacc tccacttaat
     6961 ccctttccga aatagtcgtt ggcttcgcct tccagctcga attttattcc gggagcagca
     7021 aatgcaccga aactttgtcc ggcacttccg gtaaatttga tatgtatagt atcttccggc
     7081 aatccatcac ctttgtattt actggaaatt tcataactca acatcgctcc tactgttctg
     7141 tccgtattga taatcggata agctgcagtc gttggtagca tttcctctaa gctcttttcg
     7201 gcggctttta tcaatttcaa atccaaaatc tgatcatgtt taggatgggc ttgttccata
     7261 agtttatgca atccgacttc attacctaca gccggctgat tgataaccgg gcttaaatcc
     7321 aggtgtttat atttccaatg ggtgatatta tcttttgttt tcaataaatt ttgctgccct
     7381 accatttcat tcaccgttct gaaaccgagt ttcgccatcc atgttcttaa atcttgtgca
     7441 acaaacgtaa agaaattgat gacatgctgc ggatctccgg taaaacgacc gcgtaataat
     7501 gtattttgcg tggctatgcc caccggacaa gtattcaaat ggcatttgcg catcataata
     7561 caacccatca ccactaaact ggctgtggc
//
LOCUS       DAOQII010000112         7588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5292_length_7588_cov_5.483738, whole genome shotgun sequence.
ACCESSION   DAOQII010000112 DAOQII010000000
VERSION     DAOQII010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5292_length_7588_cov_5.483738"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1172
                     /locus_tag="PLF48_06645"
     CDS             114..1172
                     /locus_tag="PLF48_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNF49039.1"
                     /translation="MSKSISNKQLFTSVKQLIDEAKKQVVRNVNSVIVFTYYHVGRMI
                     VEDEQQGTKRAAYAQQTLSNLSIALTKEYGKGYSTDNLELMRKFYLSYSQISESASRK
                     SAIQKSETTSRKFNNITDFANAFRLSWSHYVQLIKIDNKKERTFYEIEAIQNNWSVRE
                     LQRQYQSSLFERLALSRDKKGVKKLATKGQNIEQPTDALKQPYVLEFLGFKEDERYSE
                     SDLETAIISKLEHFMLELGKGFLFEVRQKRFTFEGDSYYVDLVFYNRLLKCFVLFDLK
                     IGRLTHQDIGQMQMYVNYYDRKVKTKDELPTIGIILCKEENKTVVEFTLPENNKQIFA
                     KEYKLYLPSKEELKKQLK"
     gene            complement(1284..1595)
                     /locus_tag="PLF48_06650"
     CDS             complement(1284..1595)
                     /locus_tag="PLF48_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNF49040.1"
                     /translation="MHFVYIIYSVKSDKYYIGETSCLEERLLQHNKGYFKNAYTKISS
                     DWELKAAFKVDNCTSARTVERYLKSKKSRIYLEKLIAEEAFKINFKKDVLEKFGIDIK
                     C"
     gene            complement(1637..1709)
                     /locus_tag="PLF48_06655"
     tRNA            complement(1637..1709)
                     /locus_tag="PLF48_06655"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1674..1676),aa:Phe,seq:gaa)
     gene            1978..3210
                     /locus_tag="PLF48_06660"
     CDS             1978..3210
                     /locus_tag="PLF48_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNF49041.1"
                     /translation="MKNNVVLIFILLLFSVLSSCKKDNNSFTSYGYDIGSRGQIISYE
                     LIETLDTATLSTRLNSLGAGLSSLIHLNYTISAYRVEYKSLNVFDDTVKASGVIIFPH
                     LPNNKVALSSFQHGTALKKTNVSSLHPGQEYLVNAVLAAEGGILCCMPDYLGMGTGDG
                     LHKYLNPTEESNSVIDILRAARYLNADLHHTQLNGQVFIFGYSQGGHATLAAQRDIET
                     KYNREFTLTASAPMAGPYCLSRTSQFDVMIDSVYYPNPFYLPYIAVSVYEATRGMVAY
                     STIFKKPYDSLIPAIINGQYSESYANARFPYYISNIVKDSVKNSARNNPNDLIRSTLR
                     TYDLIDDWTPHVPTRFYHCEGDDNVFYDNATCAVNTFLSRGGNVELANMGPSNHTDCA
                     PPTFILARTWIYELKRID"
     gene            3295..4695
                     /locus_tag="PLF48_06665"
     CDS             3295..4695
                     /locus_tag="PLF48_06665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF49042.1"
                     /translation="MLYLKKYFVFLSVLFVFYSCKQEKKTTFNAEKNPLKDTAAIQEY
                     YCTDTNKCYFLDIEVDTMSILEKEFGKRDNLQKLFRQYKLSSMQYDQFMEEAGEYYDF
                     KNIKPKQKYFIIADENDEIKPLKFLIFKISDFEYIITDCTDTTKIELEIPAVASGKKK
                     NKRKKTTYFTPLHIRLFEKNIDTLEIKLNAVITSSVQKTFKENNIDIKLMDKINQAFG
                     SRFSLSQVRKNDTLQFIYDQCSINGQFIDLGHLKAVAVKQKKRSYYAIDYFLEDDSSW
                     QYVDDKGKYLKVAFLKSPLQKGGAIVSRYNLTRFHPILLEVKPHLGTDFAAPQGSPIV
                     ATASGIVSACEFKQYNGNYVKIRHDRMYETQYLHMSKFARGMRSGVRVQQGQVIGYVG
                     STGLATGPHVCYRFWKNGEQVDPLKEKLNVVRQMPKEELPVYEAFVAKIKQELNGIEL
                     SGFQPSKQDTTVLGKK"
     gene            4700..5887
                     /locus_tag="PLF48_06670"
     CDS             4700..5887
                     /locus_tag="PLF48_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49043.1"
                     /translation="MLNRATWLLTLLFIFSTQHGKSNNPRQFISFNDTLKLKFWNDFI
                     LIPIKTDSLESYLIFDNGASISALDSIWLSKTGVADANNMHVKITDANSKSMYNKLHK
                     TQLQIGSFHFRDMSVIEIKVNHLLTGCQVPFAGLLGADIINQLNWNFNFDDSTLVISK
                     IPFKQSGTVISYYTYLNNLHYTEIQFYGNKKARANIDFGYSNETILFPNTYLSAFSGK
                     KAVIKYGQTGISLSGLDDFDSTFILPNNKCSISGKLLIDSSSFEFSANEKEIRIGNPV
                     FRKFNIILNTTTQEYILSARKLPYHRQSLFYGISLYWMYGKCRIINILTNESIKINDL
                     HIDDEIIKINKQNVSDFMDNCALYQLLKISQNAGKSIEITLANGKSVLLQPMPLRPNY
                     ILP"
     gene            5934..6494
                     /locus_tag="PLF48_06675"
     CDS             5934..6494
                     /locus_tag="PLF48_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF49044.1"
                     /translation="MKKILTLSCLIFTLFLANAQFPAIGTKAPDLTFNNPEGKPISLS
                     SLKGYVVLLDFWASWCRPCRMANPGVVQLYNKYKDQKWAKGIKGFTVFSFSLDMNTQA
                     WTNAIAQDGLTWPYHASDLKGWNSAGGAKYGINSIPCTFLIDETGYIIAIRPPHNLIE
                     EQLNKRLASAKSSNTTKTKTTKSKTK"
     gene            6569..7159
                     /gene="grpE"
                     /locus_tag="PLF48_06680"
     CDS             6569..7159
                     /gene="grpE"
                     /locus_tag="PLF48_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNF49045.1"
                     /translation="MVNNKDKKQGINDNSTENEQVTPESQGTENQSESQQQSDNIEQN
                     DAEAVVSKLQEELKDSKEKYLRLFAEFDNYKKREAKEKREWIKTAGQDIVRDLLPVLD
                     DIQRANKTLDADKNIETYAGGAKLIFDKLQKTLQQKGLKSIESIGKPFDVEYHEAIAE
                     IPVADSNQKGKIVDEVEAGYTLNDAIIRYAKVVVGK"
     gene            7291..>7588
                     /locus_tag="PLF48_06685"
     CDS             7291..>7588
                     /locus_tag="PLF48_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNF49046.1"
                     /translation="MSKRDYYEVLGVSKGASADEIKKAYRKLALQYHPDRNPGSKEAE
                     EKFKEAAEAYDVLCNEEKRARYDRFGHQGMGGGFGGGGYGGGMNMDDIFSQFGDI"
BASE COUNT         2623 a         1389 c         1357 g         2219 t
ORIGIN      
        1 tccattcaga gcagggagga taacctcctc tctgttttga aaaaatgttg atttatgcct
       61 ccaaaaaatt gcatttaaca cttgaactta ggctatcttt aacctataca agtatgagca
      121 aaagcatttc caataaacaa ttatttactt ccgtaaaaca gcttattgat gaagccaaga
      181 agcaagtagt acgcaatgta aactctgtaa tagtatttac gtattatcat gtaggacgta
      241 tgattgtaga ggatgaacaa caaggcacta aacgtgcagc ttacgcacaa caaaccctca
      301 gcaatcttag tattgcatta accaaagaat atggcaaagg gtattcgaca gacaatttgg
      361 agttaatgcg aaagttttat ttatcctact cacaaatttc cgaatcagca tctcggaaat
      421 ctgctattca aaaatccgag acaacgtctc ggaaattcaa caacattacg gattttgcaa
      481 atgcctttag attaagttgg tcacactatg tacaacttat aaaaatagac aacaaaaaag
      541 aacgaacatt ctatgaaata gaagccatac agaacaactg gagtgtacga gaattacaac
      601 gacaatatca atcatcttta tttgaacgat tggcattaag cagagataag aaaggcgtaa
      661 agaaattagc cacaaaagga caaaacattg aacaaccaac tgatgcctta aaacaacctt
      721 atgtattaga gttcttaggt tttaaagaag atgaacgcta ttctgaaagc gatttggaaa
      781 cagccatcat atctaagtta gaacatttta tgttagaatt aggaaaagga tttttgtttg
      841 aagtacgaca gaaacgattt acatttgaag gcgacagcta ttatgttgat ttagtatttt
      901 ataatagact gctaaagtgt tttgttttat tcgatttaaa aataggaaga cttacgcatc
      961 aggatattgg gcaaatgcag atgtatgtaa actattacga ccgaaaagta aaaactaagg
     1021 acgaactgcc tactatcggc attatattgt gtaaagaaga aaacaaaacg gtagtagaat
     1081 ttaccttacc tgaaaacaat aaacagatat ttgccaaaga atataaacta tacctgccaa
     1141 gcaaagaaga attgaaaaaa caactaaaat aatagttcaa gtgttcttaa tataaaaaag
     1201 actgccttaa gcagtctttt gtggtgtggg gcgggatcgt cccgataaat cataaattta
     1261 tcgggacgcc gacacaagga ttttcaacat tttatatcaa tgccaaattt ttctaacaca
     1321 tctttcttaa aatttatttt aaatgcttcc tcagctatca atttttccag atagattctt
     1381 gatttcttgg atttcaagta tcgttctact gttctggcgg aagtacagtt atcgacttta
     1441 aaggctgctt taagctccca atcactgctt attttagtat atgcattctt aaaatatccc
     1501 ttattatgct gcaacaatcg ctcttccaga cagcttgttt cgcctatata atatttatca
     1561 gattttacgc tataaattat gtaaacaaaa tgcattttaa tttataagaa aaaagactgc
     1621 cttaagcagt cttttgtggt gtggggcggg atcgaaccgc cgacacaagg attttcagtc
     1681 ctttgctcta ccgactgagc taccgcacct tttccgttaa aatctaacgg agtgcaaata
     1741 tacgcttttt ttgtaaaaac aaaagttctg caaccaaata agataaaatt catacagcaa
     1801 cattttgtga tatcgataaa ggattgtact caaaaaatca cctgaacttc atcatgagtt
     1861 tgagttcatc caaataaaat atatctacat aacacattga ttataagaaa gctgcttaat
     1921 tttttgtaaa attaattagt ttatttttat acctttaaac aaataatctg caaaacaatg
     1981 aaaaacaatg tcgttttaat ttttatcctc ttgcttttct ccgtcctatc ttcctgcaaa
     2041 aaagataaca acagttttac ttcctatggt tatgatattg gctcgcgtgg acaaattatt
     2101 tcctacgaat taatagaaac gctcgataca gctacactaa gtacccgact gaatagttta
     2161 ggtgctggtc tatcttcact cattcactta aattacacca tttctgctta ccgcgtagaa
     2221 tataaatcat taaatgtatt tgacgacacc gtaaaagcca gcggcgtcat catctttcct
     2281 catctgccaa acaacaaagt ggcattaagc agctttcaac acggaactgc actgaaaaaa
     2341 accaatgttt catctttgca ccccgggcaa gaatatctgg taaatgccgt actggcagcc
     2401 gagggtggaa ttttgtgctg catgcccgac tacttaggca tgggcacagg cgatggtctg
     2461 cacaaatatc tgaatccgac tgaagaatca aattctgtaa tagatattct tcgggctgcc
     2521 agatacctga atgcagattt gcaccataca caactgaatg ggcaagtttt tattttcgga
     2581 tattctcaag gcggacatgc tacgttggct gcacagcgcg atatagaaac aaaatacaac
     2641 cgtgagttta ccttgacggc atctgcaccc atggcagggc cttattgcct tagccgcact
     2701 tctcaatttg atgttatgat agattcggta tattatccca atccatttta tttaccttat
     2761 atcgcagtaa gcgtttatga agctaccaga ggtatggttg cttatagtac aattttcaaa
     2821 aagccttatg attcactgat accggctata atcaatggac aatattcaga aagctatgcc
     2881 aatgcccgtt tcccttacta tatcagcaac atcgtaaaag actctgtaaa aaactctgcc
     2941 agaaataatc caaatgattt aattcggtct accctgcgta cttatgattt gatagatgac
     3001 tggacaccac acgtaccaac cagattctat cattgtgagg gagatgataa tgtattttat
     3061 gacaatgcaa catgtgccgt gaatactttt ctgtcgagag ggggaaatgt agaactggcc
     3121 aatatgggtc caagtaatca tacagattgt gcgcctccga catttatttt agccaggaca
     3181 tggatctatg agttgaagcg aattgactaa ctattttagt atttcttagc tattttatta
     3241 tgtagaaaca ctgcgtattt acgtatattt gcacctcgta acaacaaaac tatcttgctt
     3301 tatctaaaaa aatatttcgt ttttctaagc gtgctttttg tcttttactc ttgcaagcag
     3361 gaaaagaaaa caacatttaa tgctgaaaaa aatccgctga aagacaccgc agcaatacag
     3421 gaatattatt gcaccgacac caacaaatgc tactttttag atattgaggt cgatacgatg
     3481 agcattttgg aaaaagaatt tggcaaaaga gataacttac aaaaactctt ccggcaatat
     3541 aaactcagta gcatgcaata tgaccagttt atggaagaag ccggagaata ctatgatttt
     3601 aagaatatca aaccaaaaca aaaatacttc atcattgcag atgaaaatga tgaaataaaa
     3661 cccttgaaat tcctgatttt taaaatttct gatttcgaat atatcatcac cgactgcaca
     3721 gatacgacaa aaattgaatt ggaaatccca gccgtagcaa gtggcaaaaa gaaaaacaaa
     3781 cgtaaaaaaa ccacctattt cacaccactg catatccgat tatttgaaaa aaacatagac
     3841 acactcgaga ttaagctcaa tgctgttatt acatcatccg tacaaaaaac atttaaagaa
     3901 aataatattg acatcaaact gatggataaa atcaatcagg catttggctc tcgtttttct
     3961 ttaagccagg tcagaaaaaa tgacaccttg cagtttattt atgaccaatg tagtatcaat
     4021 gggcagttta ttgacttggg gcatttaaaa gcagttgccg taaaacaaaa aaaacgaagt
     4081 tactatgcta tcgattattt tttggaagat gacagcagct ggcaatacgt agatgataaa
     4141 gggaaatacc ttaaagtggc attcctgaaa tcgccattac aaaaaggcgg agctattgtt
     4201 tcccgataca atcttacacg ttttcaccct attttattag aagtaaaacc tcatttaggt
     4261 actgattttg ctgcgcctca aggctctcca atagttgcca cggcaagtgg aattgtgtct
     4321 gcttgcgagt ttaagcaata caatggcaat tatgtaaaaa taaggcacga ccgaatgtat
     4381 gaaacccaat acctgcacat gagcaaattt gcccgtggaa tgcgaagtgg ggttcgtgta
     4441 caacaaggtc aggtaatagg ctatgtgggc agcacagggt tggcaaccgg gccgcacgta
     4501 tgttatcgtt tctggaaaaa cggcgaacaa gttgacccgc ttaaagagaa attgaatgtt
     4561 gtacggcaaa tgccgaaaga agaattacct gtttacgaag catttgttgc caagataaaa
     4621 caagagttga atggtattga attatccggc tttcagcctt caaaacaaga tacaactgtt
     4681 ttaggtaaaa agtaatttta tgctgaatcg tgcaacttgg ctactgactt tattattcat
     4741 attttccaca caacacggca aatcaaacaa cccgaggcag tttatttcat ttaacgatac
     4801 gctgaaactt aaattttgga acgatttcat cttaatcccc ataaaaacag attcactcga
     4861 atcgtatctg attttcgaca atggcgcatc aatatcagca ctcgatagta tttggctgtc
     4921 taaaactggt gttgctgatg caaacaacat gcatgtaaaa ataactgatg ccaacagcaa
     4981 aagcatgtac aataaactgc ataaaaccca attacaaatt gggtcttttc actttcggga
     5041 tatgtctgtt atagagataa aagtgaatca tttgcttacc gggtgtcaag taccttttgc
     5101 aggactttta ggtgcagata ttatcaacca gctaaactgg aattttaatt ttgacgactc
     5161 cacattagtt atttctaaaa ttcctttcaa acaaagtggc accgttatct cctattatac
     5221 ttatctgaat aatttacatt atactgaaat acaattttac ggcaataaga aagcaagagc
     5281 aaatattgat ttcggatata gcaatgaaac gattttattt cccaatacct acctgtctgc
     5341 tttttccggc aaaaaggctg ttataaaata tggtcaaacc ggcatttcgc tttcgggttt
     5401 agatgacttc gattctacat ttattttgcc aaataataaa tgttcgattt ccggcaaact
     5461 acttattgac tcttcaagtt ttgagttttc tgctaacgaa aaggagatac gtattggaaa
     5521 tccggtattc agaaaattca atatcatact gaatactact acccaggaat atatactttc
     5581 tgcacgaaaa ttgccttacc atagacaatc tttattttat ggcatttctt tgtattggat
     5641 gtatggaaaa tgtaggatta taaatatttt gacaaacgaa agcataaaga taaatgacct
     5701 tcatattgat gatgagatta taaagattaa caagcaaaat gtcagtgatt ttatggataa
     5761 ttgtgcatta tatcagttgc tcaagattag tcaaaacgca ggcaagtcta tagaaataac
     5821 cctggctaat ggcaaatcgg tgttactgca acccatgcct ttacgaccaa actatatctt
     5881 gccttgaatt caggcataaa atttgtctat atttgtgtat taacttaatt acaatgaaaa
     5941 aaatactgac tttatcttgt ttaattttta cgctttttct tgccaatgcc caattcccgg
     6001 ctataggcac taaagcaccg gatcttactt ttaacaatcc ggaaggcaaa cccatttcat
     6061 tgtcatcact taaaggctac gtagtacttt tggatttttg ggcttcctgg tgtcgccctt
     6121 gccgtatggc gaacccgggt gtggtacaat tgtataataa atacaaagat caaaaatggg
     6181 ctaaaggcat taaaggtttt acagttttct cattttcgct ggatatgaat acacaggcat
     6241 ggacaaatgc cattgctcaa gacggcttaa catggccata ccatgcttct gatcttaaag
     6301 gatggaactc ggcaggtggt gcaaaatatg gcattaactc cataccttgt actttcctta
     6361 ttgacgaaac cggatatatt attgctatcc gcccgcctca taatctgatt gaagagcaac
     6421 taaataaacg attagcttct gctaaatcaa gtaatacgac taaaacaaaa acaactaaaa
     6481 gtaaaacaaa ataaaattta gtgtcaaatt gtcttaaatt atcctttggc aagtattttg
     6541 ccaaaggatt tttagtaaat agtaacttat ggtaaataat aaagataaaa aacagggaat
     6601 aaatgacaac tctactgaga acgaacaagt tacaccagaa tcccagggaa ctgaaaatca
     6661 gtcagaaagt cagcagcagt cggataatat tgaacaaaat gacgctgagg ctgtcgtaag
     6721 caaattacag gaagaactaa aagacagcaa agagaaatat ttgcgtcttt ttgccgaatt
     6781 tgacaattat aaaaaacgcg aggccaaaga aaaacgtgaa tggataaaaa cagctggaca
     6841 agatattgta agagatttat tgccagtttt ggacgatata caacgagcaa acaagactct
     6901 ggatgctgat aaaaatattg aaacatatgc cgggggggct aaactcattt tcgacaaatt
     6961 gcagaaaaca cttcaacaaa aaggattaaa atcgatagaa agtatcggga agccatttga
     7021 cgtggaatac catgaagcta ttgcagaaat tccggttgca gacagcaacc aaaaaggcaa
     7081 aattgtagat gaagtggaag ccggctatac attgaatgat gccatcatcc gctatgccaa
     7141 ggtagtagta ggaaaataat tgtttgtaat aaattattgt agttagtaat gggctttttt
     7201 attcatgaaa tatatctcta acagtccgtt tttattcaat atcacacaac atttaaccag
     7261 taacaaatta catttaacca ataaccaacc atgtccaaac gcgattatta cgaagtactt
     7321 ggtgtttcaa aaggagcatc agccgatgaa ataaagaaag cttaccgcaa actagcttta
     7381 caatatcatc cggaccgaaa cccgggaagt aaagaggcgg aagaaaaatt taaagaagct
     7441 gccgaagctt atgatgtgtt gtgcaacgaa gagaaacggg cacgttacga tcgttttggt
     7501 catcagggca tgggtggcgg tttcggtggc ggcggttatg gcggtggtat gaacatggat
     7561 gatatcttct ctcaatttgg cgatatct
//
LOCUS       DAOQII010000113         7532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5362_length_7532_cov_5.052160, whole genome shotgun sequence.
ACCESSION   DAOQII010000113 DAOQII010000000
VERSION     DAOQII010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5362_length_7532_cov_5.052160"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1604)
                     /locus_tag="PLF48_06690"
     CDS             complement(174..1604)
                     /locus_tag="PLF48_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF49047.1"
                     /translation="MKKLSLSLFILYHCISFAQTKMKTFYESNQEIKQVHEEYEYIIV
                     NDLIVKNGQFTAYDQFGDYLQIGNYSHDKKTGEWFFYTNNKLDSSGSYLNDMPDGKWK
                     YYTAFERLTKEGTFRAGVRVGKWDFYNSSGNKSYTYNYLPERTAIEVYNENSTTTIIG
                     ESIENGILNGTFYYYYPDGQLRCKVVYKNSAFTDSIKSYYKNGYKLCVAIYDSSSIKE
                     KTVFNTDGKINYEIQQLKDAVFLYRYYYPNGNKEYEIEKNDSVFHSYSAWDLSGRPLD
                     CGNYKDGNGKSIQYNLGIKDFEANYSNYKLDGEYITYFSNGNIKNKQFYKNGAKVGIW
                     EYYLENGKLQASKDIATGIITNYSKDRYDESVAKTLNSKAEEDIRPSYPGGVVNLMKY
                     LSKNIIYPTIAKENGLEGKVIAKFMVNQIGSITNIEILKDGVGGGCAEEAIRVIKKMP
                     LWEPGFNDGFPVNDYYILPIVFRLAN"
     gene            1730..2500
                     /locus_tag="PLF48_06695"
     CDS             1730..2500
                     /locus_tag="PLF48_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49048.1"
                     /translation="MMRNAYFILSLLLLFTSFAQAQNEIKVTGKVVDENGYPLPFTDI
                     ANRRKQLGFSSSKDGSFSTTMLITDSLVFLRKNFNAAKLTLRDSVAKNEYYVVVKMER
                     MAIELSEVQINAIRTHQQIRTEINRLHVENTDVNPDARPLLNPISYLYELISKKEKEK
                     RVVSQLVTEERKRMVLKDLFRLYNAYDIIDLDEDEYDRFITYLNLPYEYLQHTSDYDL
                     AVSIRRMYASYHKDTKSSFGKPIYPAALDDLEKIKHKE"
     gene            complement(2911..3558)
                     /locus_tag="PLF48_06700"
     CDS             complement(2911..3558)
                     /locus_tag="PLF48_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49049.1"
                     /translation="MKTNNNNQTFLSQLLKMAIIPILCFIAISITTISCTKNKCNYSP
                     VCTVQDSVNVSTGLDSVSLVDPNWIASSNPYPLGTPVLVIPAHPGYEPTPIAGTNAGW
                     INMTGSACCANILGNYTYETTFPIAPNILSFSCDFAIAYDDSLVSLELEDPSGNMIPL
                     TEPTHTVALDRLGTNIGTVFNNPAIGTWKIKATIKTADHGTGFLLSGYIKRTKPC"
     gene            complement(3587..5332)
                     /locus_tag="PLF48_06705"
     CDS             complement(3587..5332)
                     /locus_tag="PLF48_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49050.1"
                     /translation="MKKLSKFILLLLVVFAGFNMQAKPAKAKTDSSTTKNHSLMVSAR
                     AGYDFPWYYNNTPYIKYKGNVTAGISVDYYWKWIGAGIDVDFIRNKPKSVYPTTNLQN
                     AAPIPITNFTKSEQKITRVFYGIGPDFRYYTKNKKFVAELNTRVGLSLVRGGRTFLQE
                     TTTLSQDVLNFHAGYDDKKALSVKGQVRLSYFFHKNIGVHIGSYYMRHFNVENKIDPA
                     LGIVAMYQPVSNAIVPGMNTYNGLPQVIKKPCDCDIASVGVFAGITYNLDFGKGKEKS
                     GNHTLVVTAKDKYTHELLPNTVVSLKDVSGNTIQTATTDGKGVVRFENIKPDNYKIEG
                     ALYDIALENNSVSKTELANKKVTEKEILYGDRNFIIKGRVFECNSTKPIAGINVVLEN
                     NDLAYKKSTMTDQDGNYILQLPATGTYNLYGKKASYFSQVERVTAGNYNRDKNLFVKL
                     EMCSEYVDCGKAIKLNNILFDLDKYEIKEAAKVELNKLVRFMKDNPDVRVELSSHTDC
                     RASTEYNQTLSQNRANASVDYIVSQGISRDRIIGKGYGESKLLNKCADGVSCTEAEHA
                     INRRTEMKVLCPEGK"
     gene            complement(5358..5960)
                     /locus_tag="PLF48_06710"
     CDS             complement(5358..5960)
                     /locus_tag="PLF48_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49051.1"
                     /translation="MKTKYVKLFSLFLIAFISFLSTGCGSSSTDPTPCSTTGTYFQAI
                     YSNTATAAGNTDMVTYDSEIHEYSFRLSANKTVCSVGYQSQPAISSLPYTIELRDSTT
                     HTTVFNISSTFSSSATSYVSVPSNPVLTTGHVYTLKRIITSYGGLITNTVGRMAISTS
                     FTPLSFPTSSGDMSIIGSKYYQNATAVTDLGIPFIDIVFK"
     gene            complement(6013..6672)
                     /locus_tag="PLF48_06715"
     CDS             complement(6013..6672)
                     /locus_tag="PLF48_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49052.1"
                     /translation="MKTKIISVQMAIIIFIILVLQACVPTSSTPLASGTFNVNQTVKT
                     DAYEIKDTTVVIAGDSIEFMLSHINQHFIDYVVLGISTSSNSFSQLQVQKHFYDYSMN
                     AYDQTNNVPSLYSVNTINAGVLINNSYTANNWIAFQPIGGKYVGQATLFVSSDASENM
                     FNGVRFNTNEYIVLRKQTGSVFQYYWILVQANETSPASGIHSVKILNGKVQSNSITTG
                     I"
     gene            complement(6744..>7532)
                     /locus_tag="PLF48_06720"
     CDS             complement(6744..>7532)
                     /locus_tag="PLF48_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49053.1"
                     /translation="RIYFPPYSFTRNGVTVTGNVTLELIELQDAGNMAATKKTTMGLL
                     PDGNKAVLVSGGEFNINVKQNGNPLDLVIPITVTTPANLTDSGRANMDLFNGVIDAKG
                     DLTWEKVTDSTGHPLVQTIDAINPTNGQMELHYNTLVSHFGWTNIDRFYSDPRPKTTM
                     LVSVPEGYNYDNCALYIKYVGLGSSLARLDTYNSSTKLFSEHYGQIPIGTECHLIFCT
                     EENGKWKYATKRITIAAGATYSVTEAEMTVGSQADYVGHVTLLR"
BASE COUNT         2219 a         1402 c         1309 g         2602 t
ORIGIN      
        1 atttctcatt ttttcctcca acttaacaag gaggaggaat taaaaggagg tggttatata
       61 taaaaaatca actattttat cccgaactca agttagtagc ccaagttttg gctaaaacca
      121 aatttggtca aattttctca ggatgataca aattatcagt attgtctata ttcctaatta
      181 gctaatctaa aaacaatagg aaggatataa taatcattta ccggaaatcc atcattaaag
      241 ccgggctccc agagtggcat tttcttaatc acacggattg cttcttctgc acatccgcca
      301 ccaacaccat cttttaaaat ttctatatta gttatactgc ctatttggtt gaccatgaat
      361 ttagcaatta ctttcccttc tagtccattt tctttggcaa tggttgggta aatgatgttt
      421 ttacttaaat atttcatgag atttacaaca cctcccgggt aagatggcct tatatcttct
      481 tcggctttgg aattaagtgt ttttgctacg gattcatcat atctgtcttt actataattt
      541 gtaataattc cggtagctat atcttttgat gcttgcagtt ttccgttttc cagatagtat
      601 tcccaaatac cgacttttgc accattctta taaaattgct tattttttat attgccatta
      661 ctgaaataag tgatgtattc accatccagc ttataattac tatagtttgc ttcgaaatct
      721 tttataccca gattgtattg tatcgatttt ccgtttccgt ctttgtaatt cccgcagtct
      781 agcggtctac ctgataaatc ccaagccgaa taactgtgaa atacagaatc attcttctcg
      841 atttcatact ctttattgcc atttggataa tagtatctat acagaaacac agcatctttt
      901 aattgttgaa tttcataatt gatttttcca tccgtattga aaacagtttt ctctttaata
      961 cttgaagaat cataaatggc aacacataat ttgtacccat ttttataata agatttaatg
     1021 ctgtctgtaa atgcagaatt tttatagacc actttacatc tcagttgtcc gtcaggataa
     1081 tagtaataaa atgtgccatt taaaatgcca ttttctatag attctcctat aatcgtcgtt
     1141 gttgaatttt cattatagac ttcaattgca gttctttccg gtagataatt atatgtgtag
     1201 gatttatttc cactggaatt gtaaaaatcc catttgccaa cacgaactcc cgcacggaaa
     1261 gtcccttcct ttgtcagtct ttcaaatgcc gtatagtatt tccatttgcc gtcaggcata
     1321 tcattcagat agctgccgct ggagtctaat ttgttattgg tataaaaaaa ccactccccg
     1381 gtttttttgt catggctgta atttcctatt tgcaaatagt caccaaattg atcatatgct
     1441 gtgaattgtc cgtttttgac aatcagatca tttacgatga tgtattcata ttcttcatgc
     1501 acttgtttta tttcctgatt actctcgtaa aatgtcttca tctttgtttg ggcaaaggaa
     1561 atacaatggt ataatataaa taagctaagg gatagttttt tcatgatatt caatatacaa
     1621 atcaaaaata atacaaaaaa tgtacattac aggtttttat ctgctttctc tcaacataaa
     1681 ttaacataat cgcttttttc gctttgcgat aacattgtat atttgaaacg tgatgcggaa
     1741 cgcttatttc atactctcgc tattgttgtt atttacttca tttgcgcaag cacaaaatga
     1801 gataaaagta accggaaagg tagttgacga aaatggctat ccattacctt ttacagatat
     1861 tgccaaccga cgcaaacagc ttggtttttc atcttctaaa gatggttctt tcagcacgac
     1921 tatgctgata acagattcac tggtttttct tcgaaaaaat ttcaatgcag ccaagcttac
     1981 gctgagagat tctgtggcta aaaacgaata ctatgttgta gtgaaaatgg agcgcatggc
     2041 aatagaactt tccgaggtac aaataaatgc catccgtacg caccagcaaa ttagaaccga
     2101 aatcaacagg ctgcatgtcg aaaacacgga tgtcaatccc gatgcacgtc ctctgctcaa
     2161 tcccatttct tatttgtatg aactgattag caagaaagaa aaagaaaaaa gggtggtttc
     2221 ccagttagtt actgaagaac gaaaacgcat ggtacttaaa gatttattca ggctgtacaa
     2281 tgcatatgat attatagatt tggatgaaga tgaatacgat cgatttatca cgtatctcaa
     2341 tttgccctac gaatatttac agcatacttc cgattacgac ttggcggtat ccatacgacg
     2401 catgtatgct tcttaccaca aagacaccaa gagttcattt ggcaagccca tttaccctgc
     2461 agccttggat gatttggaaa aaattaagca taaagaatag cttgccctcg tgcggtttaa
     2521 accacataaa agcatagcat tcatttttaa tcatcaagag ttgtgtggtg taaatcagct
     2581 ttgctagtca agttaaaaca tagttcgctt tgacgttcct gtatattctt attcttatct
     2641 ccattttgtc ttaaacatat tcttaatcct aatgaaatct atgcagctat gtggtaaaaa
     2701 ctatcatcca aaccgcataa gagaaaagac tataaatatt cactcactta agaattaatt
     2761 acaattttta tcctgtccaa tttcattttt tatgaaataa aaaagagaga ctatttcgag
     2821 aatgattatt ctgaaacaga ctcacaataa aatagaatga aataaaaacg cctactctgt
     2881 ttcaggtttt cggcttcccc tgatttttcg ttagcaaggt ttggttcttt tgatataacc
     2941 acttaataag aaacctgtac catgatctgc agtttttatt gtggctttta tcttccatgt
     3001 tccaatagct gggttattga aaactgtacc gatatttgta cctaatctgt ctaatgcaac
     3061 ggtatgtgtt ggttcggtta aaggaatcat attgccactc gggtcttcca attccaatga
     3121 aactaaacta tcatcgtatg ctattgcaaa atcgcatgaa aaactaagaa tattaggtgc
     3181 aattggaaaa gttgtttcat aagtataatt acctagtata tttgcacaac aagcacttcc
     3241 tgtcatgttt atccaaccag catttgtacc agcaattgga gttggctcat atccaggatg
     3301 agctggaatt acaagtactg gagtgcctaa tggatatgga ttactcgatg caatccagtt
     3361 aggatctacc aaactaacag aatctaatcc ggtagataca tttacagaat cttgtacggt
     3421 gcaaaccggg ctatagttgc atttgttttt tgtgcaagaa atagttgtta tgctaattgc
     3481 aataaaacac agaatgggta tgatagccat ttttagcaac tgtgataaaa atgtctggtt
     3541 attattattc gttttcataa aaattgtttt ttagattttt gattaattat tttccttcag
     3601 ggcaaagcac tttcatttcc gtgcgacggt tgatggcatg ttcggcttct gtacacgata
     3661 cgccatctgc acatttgttc agcagtttgc tttcgccata gccttttcca ataattctgt
     3721 ctctcgatat gccttgtgat acgatataat ccacggatgc attcgcacgg ttttgtgata
     3781 aagtttgatt gtattccgta cttgcacgac agtctgtatg cgagctcaac tctactctta
     3841 catccggatt gtctttcata aagcgtacca gtttgtttag ttccactttt gccgcttctt
     3901 tgatttcgta tttatccaaa tcaaacaaaa tattattaag cttgatggct ttaccacaat
     3961 ccacatattc cgaacacatc tctaatttca cgaacaagtt tttatcgcgg ttataatttc
     4021 ctgcagttac acgttccact tgcgaaaaat aactggcttt tttgccgtat aaattatagg
     4081 tgcctgtggc aggcaattgt aggatgtagt taccgtcctg atcagtcatg gtagattttt
     4141 tatacgctaa atcattgttt tccaaaacca catttatacc cgcgatcggt ttggtagagt
     4201 tacattcaaa tactcttcct ttgataatga aatttcggtc gccatacaag atttcttttt
     4261 ccgttacttt tttgtttgct aattcagttt tggaaacact gttgttttct aatgcaatgt
     4321 catataatgc accttctatt ttataattat ccggtttgat attttcaaaa cgtacaacac
     4381 ctttgccatc tgtagtggca gtttgtatcg tattgccgga tacgtctttt aaagatacaa
     4441 ctgtattggg taataactca tgggtatatt tgtctttggc agttaccacc aaagtatggt
     4501 tgcccgattt ttcttttcct tttccaaaat ctaaattata ggtaatgcct gcaaacacgc
     4561 ctaccgatgc gatatcgcaa tcgcatggtt ttttgatgac ttgtggtaaa ccgttatagg
     4621 tattcatacc cggaacaatg gcattgctta ccggctgata catggcaacg atacctaaag
     4681 ccggatcgat tttgttttcc acattaaaat ggcgcatata ataacttcca atgtgtacac
     4741 ctatgttttt gtggaagaaa tacgataagc gaacttgacc ttttacggac aaggcttttt
     4801 tgtcgtcata tcctgcatga aaattcagta catcttgaga gagtgtagtg gtttcttgta
     4861 aaaatgttct tccgcctctc actaaagaca aacccacacg cgtattcaac tctgctacaa
     4921 atttcttgtt tttagtatag tatctgaagt ctggaccaat gccataaaaa acgcgcgtaa
     4981 ttttttgttc agatttcgta aaattggtga taggtatagg tgcggcattt tgcaaattag
     5041 tggtcggata cacgcttttg ggtttattgc ggataaagtc cacgtcaata cccgcaccta
     5101 tccacttcca gtaataatct acagatatcc ccgcggtaac gttgcccttg tatttgatat
     5161 aaggcgtgtt gttgtagtac caagggaaat cgtaaccggc cctggctgac accattaggc
     5221 tatggttttt agttgtactg ctgtctgttt ttgctttggc aggctttgcc tgcatgttga
     5281 agcctgcaaa tacgacaagc aacaacaaga taaattttga tagctttttc attttttttg
     5341 attttgaatt gtgattatta tttaaatacg atatcgataa aaggaatacc cagatcggtt
     5401 actgccgtag cattttgata atattttgaa ccaataatgc tcatatctcc tgaactggtt
     5461 gggaatgata atggcgtgaa cgaggtggaa atagccattc ttcctactgt attcgtaatc
     5521 agtccaccat agctggtaat gattcttttt agcgtataca catgtccggt tgtaagtaca
     5581 ggattagatg gaacagaaac ataactggta gccgatgaac tgaacgtgct ggaaatattg
     5641 aatacagtag tatgggtagt gctgtctcta agttcaatag tatatggcaa actggaaatg
     5701 gcaggctgac tttgataacc cacgctgcaa accgttttgt ttgcggaaag ccggaaggaa
     5761 tattcatgaa tttcggagtc ataggttacc atatccgtat ttccggcagc agttgctgta
     5821 ttgctgtata tagcctgaaa ataagttcct gttgtactgc aaggtgtcgg gtctgttgaa
     5881 gaagagccac atccggtgct taaaaatgaa ataaatgcaa taaggaagag gctgaataat
     5941 tttacatatt ttgttttcat gatgttttct tttttattgt tattgaaatt tattgattga
     6001 agtttattaa aattatatcc cggtcgtaat agagttggat tgaacttttc catttaagat
     6061 tttgacacta tgtattcctg atgctggtga ggtttcattt gcctgtacta atatccagta
     6121 atattgaaac acactgcctg tttgcttgcg taaaacgata tattcattcg tattgaaccg
     6181 gacgccattg aacatattct ccgatgcatc agaactaaca aataaggtgg cttgaccaac
     6241 atattttcca ccaatcggct gaaaggcaat ccaattattt gccgtgtaac tattattaat
     6301 gagtacgcct gcattgatgg tatttacgct atataaagaa ggaacattat tggtttggtc
     6361 gtatgcattc atggaataat cataaaaatg cttttgcact tgtaattgag aaaagctgtt
     6421 tgagcttgta ctaattccca acacaacata gtcaatgaaa tgctggttga tatggctgag
     6481 cataaactca atagaatctc ctgcaatcac cacagttgtg tcttttattt cataagcatc
     6541 tgtttttaca gtctgattta cattgaacgt accggaagct aaaggcgttg aactggtagg
     6601 gacacaagct tgcagaacaa gtataatgaa gatgattatt gccatttgaa cggatataat
     6661 tttagttttc attttcccta tctattttaa tttaaaaatt tgcctactct ttttcaggtt
     6721 ttcggcttcc cctgttttta tatttatctt aataaggtta catgaccaac ataatcagct
     6781 tgacttccaa ctgtcatttc tgcttcagtt acagaatacg ttgcacctgc agcaatagta
     6841 atgcgtttgg tagcatattt ccatttgccg ttttcttctg tacaaaaaat taaatgacat
     6901 tcagtaccaa taggaatttg accataatgc tcagaaaata attttgttga ggaattataa
     6961 gtatccagtc ttgctaatga actaccaagt ccaacatatt ttatatacag cgcacaatta
     7021 tcataattat atccttcagg aacgcttacc aacatcgtag ttttaggacg cggatcagag
     7081 tagaaacggt ctatatttgt ccatccaaaa tggctaacta aagtattgta gtgcaattcc
     7141 atttgcccat ttgttggatt tatggcatca atagtttgta caagagggtg tccggtagaa
     7201 tccgttactt tttcccaggt aaggtcgcct tttgcatcaa taacaccatt aaataaatcc
     7261 atgtttgctc tgccactgtc cgttaaatta gcgggtgtag ttactgttat tggtataact
     7321 aaatctaatg gatttccgtt ttgtttaaca ttgatattga attcaccacc ggaaactaag
     7381 accgctttat ttccatcagg taataaaccc atagtcgttt ttttagtggc agccatattt
     7441 ccggcatctt gaagttctat caattctaat gtaacattac ctgtaaccgt tacaccattt
     7501 cgtgtaaaag aatatggtgg aaaataaatc ct
//
LOCUS       DAOQII010000114         7448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5455_length_7448_cov_5.007304, whole genome shotgun sequence.
ACCESSION   DAOQII010000114 DAOQII010000000
VERSION     DAOQII010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5455_length_7448_cov_5.007304"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLF48_06725"
     CDS             <1..1020
                     /locus_tag="PLF48_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49054.1"
                     /translation="YAAGYSAGGFMCYKLACDLTNRVAAIAPNVASMLLDNLSTCVPA
                     RPMPICAFNGSADPITAYNGIPFNFPGIDSVKHFWQIKNNCDQIPVTDTLPDLQNDGT
                     RVVRYTYTNCTNATQQVFYKVLNGGHTWPGADDVFLGLIGKTTRDIDMNNTAWNFFKT
                     KEIPLQLVCDAPQNLQAVFISSDSVQVSWEAIAGVNTYRVALVDDSDRVHFFETSGNE
                     MGLTLNPAKRYKWNIASICSSGFHNWNTTRNLNQLPTSLKNVSNKILHVYPNPAADVL
                     HIDLRQNQIKSAQVIIYDEVGNIVYQEEKSLVNTLDISFLAAGNYFLEIKSDADLFLR
                     KFIKL"
     gene            1024..1335
                     /locus_tag="PLF48_06730"
     CDS             1024..1335
                     /locus_tag="PLF48_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNF49055.1"
                     /translation="MYEYTVKICIDKSIESDWKKFMLEKHIQDVLDTGHFSHAAIKKI
                     IKENESNDVEYSVEYLAASLSAIEEYREKCSPALQKDVLDKFGNQFTAEREVLIIIKS
                     F"
     gene            1356..1634
                     /locus_tag="PLF48_06735"
     CDS             1356..1634
                     /locus_tag="PLF48_06735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49056.1"
                     /translation="MEILQKDDGKEGMFYIEQDHKLLAELTYEWSGNKIIINHTGVRE
                     ELEGQGIGKKLVLKAVDWARANKIKILPICPFAKRVLENNDTYQDVLF"
     gene            1653..1868
                     /locus_tag="PLF48_06740"
     CDS             1653..1868
                     /locus_tag="PLF48_06740"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HNF49057.1"
                     /translation="MEEHDKIKTYTNGEITVVWQAGKCIHSGNCVRNLNKVFKPKEQP
                     WVRMENASSDEIRNAVSKCPSGALSIK"
     gene            1981..2853
                     /locus_tag="PLF48_06745"
     CDS             1981..2853
                     /locus_tag="PLF48_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNF49058.1"
                     /translation="MQMTIESELILNPDGSVYHLNLLPEDIADTILLVGDPDRVEEVS
                     RYFDKMEVQKQKREFVTHTGYVGNKRVTCLSTGIGTDNIDIVITELDALVNMDLKTRQ
                     PKATLSALNLIRIGTCGALQEDIDIDTYLASEFGVGLDGLLGFYDFSNTDEEEAMLAP
                     IVPIVSKLGVHPTIIKASDKLLNTIAKDVRWGVTLTNCGFYGPQGRTVRIAPKDKKYI
                     DELSAIMLAGNRKLTNMEMETSGIYGLAKLMGHHGLSLNAIIANRVRGIFSKDPHAAT
                     DKLIRFVLEKITEI"
     gene            2863..3096
                     /locus_tag="PLF48_06750"
     CDS             2863..3096
                     /locus_tag="PLF48_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNF49059.1"
                     /translation="MEENSDIQKQLLLDIVKTQMPYGKFKGTLLCDIPVHYLEWMART
                     GFPKNKLGSQLATVYEIKANGLTEIIDKLKKMV"
     gene            3206..3487
                     /locus_tag="PLF48_06755"
     CDS             3206..3487
                     /locus_tag="PLF48_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNF49060.1"
                     /translation="MKRKIKYYRLVWLKYDLPAGLSVFLVALPLCLGIAYISNAPIYA
                     GILSGVIGGIVVSLISGSALSVSGPAAGLSTVIAAAILSHGDYRVFYYV"
     gene            3514..4755
                     /locus_tag="PLF48_06760"
     CDS             3514..4755
                     /locus_tag="PLF48_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNF49061.1"
                     /translation="MGVLKLGFITNYIPSSVIKGMLAAIGILLISKQIPVALGYNKPD
                     FWQSGFLDLFTTNDFLTNINEFNHHVTRGVVIITLFSIGAYWLFSNTSLIKIKSIPIP
                     LIIVIIGIVLYLIQFYYVDEGAVKPSQLVNIPDNMFAQLQFPKFSMLFSSVKVWKDGI
                     TIGLLATLETLLCVEAVDKLDKHNRITPINRELIAQGVGNILCGMVGAIPITAVVVRG
                     SANINAGARTKLSAFTHGVFLLLSVLFIPFILNLIPLASLAVILIITGYNLTKVSLFK
                     NMFKLGWEQFIPFVATILVILFTDLLMGVSIGLLISVYFIIQHNFTEQYKVEDKFEDG
                     HKIIRIFLNTNVSFLNKVKLRDTLENIPDHSKLLIDGRNCRFIDYDILEIISEYETKA
                     KDRHITMQLEGIQRVETAAMH"
     gene            4824..5858
                     /locus_tag="PLF48_06765"
     CDS             4824..5858
                     /locus_tag="PLF48_06765"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49062.1"
                     /translation="MLDKYLQILLEKHSCVIVPGFGAFLAHYKPAEVNLKENKIYPPS
                     KSVAFNKTLQSNDGILANAIAETEKIGYNDALQKIAIEISFWENSILQNKSLLLKNIG
                     RFIVDENRNLIFAPFDTKNYLAESFGLQAISMEPIKRLKDTVKPITENEIKKSKFSFS
                     ILNAMLAITVLTFIVLTTVVNVLQSGTEQVSLASLFPFSSSVPAIKQEHVALQPKKKE
                     ITAEPPKYVVVDNVSYPTAIAKEENETTVEISNQPATTQIASSAAMAKNSTGKTYVAI
                     GAFFSEASTQDVVKKAESEGFTTKLYKDAKRYKVLIELNKSDLARKLPLIKSEVNANA
                     WVYCSDCTIP"
     gene            5868..6527
                     /locus_tag="PLF48_06770"
     CDS             5868..6527
                     /locus_tag="PLF48_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF49063.1"
                     /translation="MIVCNHIVKAYESLQVLKDVSFQIEKGELACIVGPSGAGKSTLL
                     HIIGTLDKADAGDVIIDGQSINELLKQQDVVAEFRNQKLGFVFQFHHLLPEFTALENV
                     CIPAYIAKKSEAEATRRAKELLDFMGLADRMQHKPNQLSGGEQQRVSVARALINNPLV
                     VLADEPTGNLDSERSEELHQLFFDLKKQYHQTFVIVTHNNSLTLQADKVFSMKDGELL
                     K"
     gene            complement(6533..6606)
                     /locus_tag="PLF48_06775"
     tRNA            complement(6533..6606)
                     /locus_tag="PLF48_06775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6570..6572),aa:Arg,seq:tcg)
     gene            6687..7226
                     /locus_tag="PLF48_06780"
     CDS             6687..7226
                     /locus_tag="PLF48_06780"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNF49064.1"
                     /translation="MNPRTVAETLNIHTELVMPGHTNPIGNLMGGNLLSWMDVCAGIS
                     AIKHSGGISVTASLDNVSFNLPIKMGDIVVLKSCVTRAFNTSMEVICEVFIKNVKTLD
                     ETKSHEAFFTFVSLDFESKPIHVPPVIPETERENALYESALQRRELRLINAGKLNAKD
                     AIYLKRELMKDWDDTENVI"
     gene            complement(7254..7336)
                     /locus_tag="PLF48_06785"
     tRNA            complement(7254..7336)
                     /locus_tag="PLF48_06785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7301..7303),aa:Leu,seq:taa)
BASE COUNT         2408 a         1307 c         1521 g         2212 t
ORIGIN      
        1 tatgcagccg gctattcggc aggtggattt atgtgttata aacttgcctg tgatttaaca
       61 aacagagttg ctgcaattgc acctaatgtt gcaagcatgt tgctcgacaa tctttctact
      121 tgcgtacctg cccgtcccat gccgatttgt gcctttaacg gaagtgcaga tccaataacc
      181 gcttataatg gcattccgtt taatttcccc ggtatagatt cagtaaagca tttttggcaa
      241 ataaaaaata attgtgatca aattccggtg acagatacct tgccggattt gcaaaatgac
      301 ggcactcgtg ttgtacgcta cacgtatacc aactgtacga atgccacaca acaggtattt
      361 tataaagtct taaacggagg gcatacctgg ccgggagctg atgatgtgtt tttgggatta
      421 atcggtaaaa ctacccgcga tattgatatg aataatacgg cgtggaattt ttttaaaact
      481 aaagaaattc ctttacagtt ggtttgtgat gcacctcaaa acctgcaagc tgtttttatt
      541 tcaagcgatt cggttcaggt cagttgggag gctattgcag gggtaaacac gtatagagta
      601 gcattggttg atgacagtga tcgggttcat ttttttgaga caagtggaaa tgaaatgggc
      661 ttgactttaa atccggctaa aagatacaaa tggaacattg cttcaatatg cagcagtggt
      721 tttcacaact ggaatactac acgaaattta aaccaattac ccacttcact aaaaaatgtt
      781 tccaataaaa ttttgcacgt ttatcccaat ccggctgcag atgtcttgca tatagattta
      841 cgacaaaatc agataaaatc agctcaggtg attatttatg atgaagttgg taatattgtt
      901 tatcaggaag aaaaaagttt ggtcaatacg ctggatattt cttttttagc agcaggtaat
      961 tattttttag aaattaaatc tgacgcagat ttgttcttaa gaaaatttat taaattataa
     1021 tatatgtacg agtacactgt gaaaatatgt attgacaaaa gtatagaatc tgactggaaa
     1081 aaatttatgc tggaaaaaca tattcaggat gtgctggata ccggtcattt ctctcatgca
     1141 gctataaaga aaataataaa ggaaaatgaa agcaatgatg ttgagtacag tgtggaatat
     1201 cttgctgcat ctctgtcagc tatagaagaa tacagagaaa aatgttcacc ggctttgcag
     1261 aaagatgtac tggataaatt cggcaatcaa tttactgcgg aaagagaagt actgattata
     1321 attaaatcct tttaaattta taaaaaaaac ggcacatgga aattctacaa aaagatgatg
     1381 gtaaagaagg catgttttat attgaacaag atcacaaact gcttgccgaa ctgacttacg
     1441 agtggtcggg caataaaatt attatcaacc atacaggggt tcgcgaagag ttggaagggc
     1501 aaggtattgg taaaaaactg gtactaaaag cggtggattg ggcaagagca aacaagataa
     1561 aaatcctgcc gatttgtcct tttgccaaac gagtattgga aaacaatgac acctatcagg
     1621 atgttctttt ttgaaaacaa aaaatgatca acatggaaga gcatgacaaa ataaaaactt
     1681 atacaaatgg cgaaattacc gttgtttggc aagccggaaa atgtatacat tccggtaatt
     1741 gtgtgcgtaa tctgaataag gtcttcaaac ctaaagaaca accttgggta cggatggaaa
     1801 atgcttcaag tgatgaaatc agaaatgccg tgagcaaatg cccgtctggg gcattgtcta
     1861 ttaaatagtt ttacatacat tttgttatga tttttatttc ttgcacaatt tagtgttcaa
     1921 aatccctgat ttctaggcag gattccgtat tttcgtattt ctttttttga atttaaaaaa
     1981 atgcaaatga ctattgagag tgaattgatt ttaaatccgg atggtagtgt ttaccatctt
     2041 aatttattgc cggaagatat tgctgacact attttgttag ttggcgaccc agatagggta
     2101 gaagaagttt cgcgctactt tgataaaatg gaagtgcaaa aacaaaaacg agagtttgtt
     2161 acacacacgg gatatgtggg taataaacgg gttacttgtt tatctacggg tattggaact
     2221 gataatattg acatcgtgat tacagaactg gatgcattgg tcaatatgga tttaaaaact
     2281 cgtcagccta aagcaacctt atcagcactc aatttaatcc gtataggtac ttgcggcgct
     2341 ctgcaggaag atattgatat tgacacttat ttagccagcg agtttggcgt agggcttgat
     2401 gggcttttgg gcttttatga tttttcaaat acagacgaag aagaagccat gttagctccg
     2461 atagtgccaa tcgtaagcaa attaggggtg catcctacga ttatcaaagc atctgacaaa
     2521 ctgctgaata cgatagctaa agacgttcgc tggggagtaa cgctcactaa ctgcggcttt
     2581 tacggcccac aaggacgcac ggtgcgcatt gcacccaaag ataaaaagta tatcgatgaa
     2641 ttatctgcca tcatgcttgc aggcaatcgc aagctgacca atatggaaat ggaaacttcc
     2701 ggtatttatg gcttggcaaa attaatgggc catcatggct tgtcgcttaa tgccattatc
     2761 gccaaccggg taagagggat attcagcaaa gatccacatg cggctacaga taaactgatt
     2821 cgttttgtct tagaaaaaat tacggagatt taagccagcg tgatggaaga aaattcggat
     2881 atacaaaagc agttgctctt ggatattgtc aaaacacaaa tgccctatgg caaatttaaa
     2941 ggcaccttgc tatgtgatat tccggtgcat tatttagagt ggatggcgag aacaggattt
     3001 ccgaaaaata aattaggtag tcagctggcg actgtttatg aaataaaagc aaacggttta
     3061 acagagatta tagataagct gaagaagatg gtgtaatgta gagggataag taattagtat
     3121 taagtaaaaa gtaattagca agatggaatt ggtcattggt acattagtac attgatacat
     3181 tgatacatta agaacattaa aataagtgaa gcgcaagata aaatattacc gattggtttg
     3241 gttgaagtat gatttgccgg caggattatc tgtgtttctg gtggcattgc cgctatgttt
     3301 aggtattgct tatatatcca atgcacccat ttatgccggc atattatcag gagtcatcgg
     3361 tggcattgta gtatcactta tcagtggctc agccttgagt gtttcagggc cggcagcagg
     3421 tttatccacc gtcattgcgg cggctatttt atcgcatggc gattatcgtg ttttctatta
     3481 tgtgtaatcg ttgccggtat ttttcagctt ctgctggggg tactcaagct tggatttatt
     3541 accaactata tcccctcatc tgttatcaaa ggcatgttgg ctgccattgg aatattgctt
     3601 atttccaaac aaattccggt tgcattgggt tataataaac cggacttctg gcaaagcgga
     3661 tttctcgatt tatttacaac caatgatttt cttaccaata tcaatgaatt taatcaccat
     3721 gttacccgtg gcgttgtcat tattaccttg ttttccattg gtgcatactg gttattttcc
     3781 aacacatcac taatcaaaat aaaatcaata cccataccgc tgattattgt aattatcgga
     3841 attgtacttt atctcattca attttattat gtagatgaag gagctgtaaa gccatcccaa
     3901 ttagtcaata tacccgataa tatgtttgcc caattgcagt tcccgaaatt ttctatgttg
     3961 ttttcatccg ttaaagtctg gaaggatggt attaccatag gcttactggc aacacttgaa
     4021 accctgttat gtgtggaagc tgtagataaa ctggataaac ataaccgcat tacacctatc
     4081 aacagggagc tgatagccca aggtgtaggc aatatccttt gtggcatggt aggtgctatc
     4141 ccaattacag cggtagtagt aagaggctct gcaaatataa atgccggagc ccgcacaaaa
     4201 ttatcggcat ttacacatgg tgtatttttg ctgttgtccg ttttgtttat cccatttatt
     4261 ctcaatttaa ttcctttggc ttcattagct gtaattctga ttattacagg ctacaatctg
     4321 actaaagttt cattattcaa aaatatgttt aagctgggat gggaacaatt tatcccattc
     4381 gtagctacaa ttcttgtcat acttttcaca gacttactga tgggtgtcag tataggtttg
     4441 ttgatttctg tgtactttat tatccagcat aattttacag agcaatacaa agtagaagat
     4501 aagtttgaag acgggcataa aatcatccgc atatttttaa atacaaatgt gagctttttg
     4561 aataaagtaa aactgcgcga cacactcgaa aatattccgg accatagcaa gctcttgatt
     4621 gacggcagaa actgcagatt tattgattat gatattctcg agataatctc tgaatatgaa
     4681 actaaagcca aagaccgtca tattactatg cagttagaag gcatacaacg tgtggaaacc
     4741 gctgccatgc attaaaaaaa atggcattaa aaagaatgag tttttaatct tgattttaga
     4801 ttgaaaatgc ttacttttac accatgttgg ataagtattt gcaaatattg ctcgaaaaac
     4861 acagttgtgt gatagtgccg ggatttgggg cgtttttagc gcattataaa ccggctgaag
     4921 ttaaccttaa agaaaataaa atttatccgc cttcaaagtc ggttgcgttt aataaaactt
     4981 tgcaatctaa tgatggaatt ttggctaatg ctattgctga aactgaaaaa attggttata
     5041 atgatgcctt gcaaaaaatt gccatagaaa tcagtttttg ggaaaattca attctccaaa
     5101 acaaaagttt attactgaaa aatataggaa gatttattgt tgatgaaaac agaaatctca
     5161 tttttgcacc attcgatacc aaaaattatt tagccgaaag ttttggtttg caggctattt
     5221 ccatggagcc gataaagagg ctgaaagata cggttaaacc catcacagaa aatgagataa
     5281 agaaaagcaa atttagtttt tcaatattga atgcaatgct cgcaataaca gttctgacgt
     5341 tcatcgtatt gacaactgtc gtaaatgttt tgcaatcagg aacagaacaa gtcagtttgg
     5401 cctctttgtt tcccttttca tcatcagtac ctgcaattaa acaagaacat gtagcacttc
     5461 aacctaaaaa gaaagaaatt acagctgagc ctccaaagta tgtagttgta gataatgtga
     5521 gttatcctac ggcaattgca aaagaagaaa atgaaacgac tgtagaaatt tcaaatcaac
     5581 cagccacaac acaaatagcg tcatcagctg ccatggcaaa aaacagtaca ggaaaaactt
     5641 atgtggctat tggcgcattt ttctctgaag catccacgca ggatgtggtt aagaaagcag
     5701 aatccgaagg ctttaccaca aagctttata aagatgcaaa gcgctataag gttttaattg
     5761 aattgaataa atctgactta gcacgcaaac tgccacttat caagtcggaa gtgaatgcca
     5821 atgcctgggt gtattgttca gattgcacaa taccctgatt tcagaaaatg atagtctgta
     5881 atcatatcgt caaagcctac gagagtttgc aagtactcaa agatgtatcc tttcaaattg
     5941 aaaaaggcga actggcttgt attgtagggc catcaggtgc gggcaaaagt acgctcttgc
     6001 atatcatcgg cactttagat aaagcagatg caggagatgt gattattgat ggacaatcta
     6061 taaatgagtt attgaagcaa caggatgttg tcgccgaatt cagaaatcag aaattgggat
     6121 ttgtctttca gttccatcat cttttacctg aatttactgc gttggaaaat gtctgtattc
     6181 cggcttatat agcaaaaaaa agtgaagcag aagctacacg aagagcaaaa gagttactcg
     6241 attttatggg acttgccgac cgtatgcaac ataaacccaa ccagctttcg ggcggggagc
     6301 aacaacgcgt ttcggtagcc cgtgcattga taaataatcc cttggtagta ttggcagatg
     6361 agcccaccgg caacctcgac agtgagcgaa gtgaagagtt gcatcagctt tttttcgatt
     6421 taaaaaaaca ataccatcag acatttgtta ttgtaaccca caacaatagc cttacattac
     6481 aagccgataa agtattttct atgaaagacg gagagttgct gaaataaaaa aatgaccttg
     6541 gaaggattcg aaccctcaac cctcagagcc gaaatctgat attctatcca attgaactac
     6601 aaggccattt tcagatgggc aaaggtaaaa attattttgc tatctttcac agtctgaatt
     6661 atctgtttta ctatcaaaat taaaatatga atcctcgtac agtagctgaa accctgaata
     6721 tccacactga actcgtcatg cccggacata ccaacccgat tggcaattta atgggaggca
     6781 atctgctgag ctggatggat gtatgcgcag gtatttctgc tatcaaacac agcggaggta
     6841 tttccgttac ggcaagtttg gataatgtat ctttcaattt gccaataaaa atgggcgata
     6901 ttgtggtgtt gaaatcttgt gtaacacgtg cttttaatac atccatggaa gtgatttgtg
     6961 aggtattcat caaaaatgta aaaactctgg atgaaactaa aagtcacgaa gcttttttta
     7021 cattcgtcag cctcgatttt gaatccaaac cgattcatgt tcctcctgtc attcccgaaa
     7081 cagaacgtga aaatgcatta tacgaatcag ctttacaaag aagagagttg cgcctgataa
     7141 atgccggaaa actaaatgcc aaagatgcca tttatcttaa gcgtgaatta atgaaagatt
     7201 gggatgatac ggaaaatgtg atttaaatct tcctgaaaaa aaatttataa gagtacccga
     7261 ggcgggactt gaacccgcac agccttgcgg ccacaggatt ttaagtcctg cgtgtctacc
     7321 attccaccac ccgggcaact tataaataag ttgtaaaaaa gtaggatact tttttaagat
     7381 aaactttgag cggaagacga gactcgaacc cgcgaccctc accttggcaa ggtgatgctc
     7441 taccaact
//
LOCUS       DAOQII010000115         7442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5466_length_7442_cov_4.941790, whole genome shotgun sequence.
ACCESSION   DAOQII010000115 DAOQII010000000
VERSION     DAOQII010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5466_length_7442_cov_4.941790"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1507
                     /gene="gatB"
                     /locus_tag="PLF48_06790"
     CDS             62..1507
                     /gene="gatB"
                     /locus_tag="PLF48_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNF49065.1"
                     /translation="MLDYGKYEAVIGLEVHAQLNTLTKIFAADETAFGALPNTHISPI
                     TLGMPGVLPKLNKEVVDKAIKFGLACNSEISQQTFFARKNYFYADLPKGYQISQDNNP
                     ICVGGFIEINDTGVKKKIRLHHAHMEEDAGKNNHELHADFSLIDLNRAGVPLLEIVSE
                     PDMHTPDEAYAYLHEIRKLVRYIDICDGNMEEGSLRCDANISIRLKGESILNTRVEVK
                     NMNSIRHVKKAIENEIKRQIDLVESGGEVVQETRSFHAEDGSSFVLRSKENAHDYRYF
                     PEPDLPPVLISNEKLEQLKSTMPPLPNALFLQFTQAYHLSEQDAVMLTEDRDTAYFFL
                     DMIRHSSNYKAVSNWLNGEIKSYLNDNHLKITDLKLRPEQLGEIIMLLDENKINSTGA
                     KTIFYHLLENTNQTALQAAESLNLMQSSDKDELTLWATQVLESMPDKVAEYKKGKKGT
                     INLFVGNVMKLSKGKAAPKQITQILEDLLSK"
     gene            1513..2655
                     /locus_tag="PLF48_06795"
     CDS             1513..2655
                     /locus_tag="PLF48_06795"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF49066.1"
                     /translation="MKRINYILSICLILLLIPSCKYFNGYQIDGKIQHANGEKVYLED
                     IFQTPALTIDTATISGSTFHLKNYSDKGIYRLRFGDNPDNSLFIYLDKKQKLSISADF
                     KQLYNYTISGNKASQTIAQLLNTNYQNLVQIDSANNEYMRAGAKQKDSMLGILKNKKS
                     AYLDYLHNFIKKEDNADVACFALQLLEPYKEEEIPFIIEQVKNLHESAPNSRQINLWY
                     AEVQQYEKQLLAKQEEGLPVGEIAPNIILQNPQGDTIQLKELKGKYVLLDFWASWCQP
                     CRQNNPHLVQIFEKYHFQGLEIFSVSLDDTKERWMNAIKVDKLKWQYHGCDYQKWNGK
                     AVNMYKVSSIPAVFLLDKNGKIIAKNLHGAALDAKLNELFSTASAK"
     gene            2652..4289
                     /locus_tag="PLF48_06800"
     CDS             2652..4289
                     /locus_tag="PLF48_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49067.1"
                     /translation="MKKQTTLDIAFIFGLSLCCHFLFSKYGFNPTDEGFVLSSSNRIL
                     HGQIPHIDFSSLRPLGYAYLHIPELLISKRYMFLVSRYIFWLEQVLIAFLWVRLVQQY
                     LGVFQNAVFQYILMFTCLVFNVHYFPVAVLHTIDGLLFAVAGLFFLQKKSVILNLIGA
                     LFFGFSVICKQGFIVMIPVAFILFWNKRITLLLSFLPVLVYVILLYMKGGATDLKMQL
                     TGHTEIVDIGFKSYFFNLYFWLGVTTVIIVRRFKFFHIYWFFQLLLLCFLLVRFHYHG
                     KLLFFYSGLLTAEIMIALINIKIPLYSMEPDKVYFENEEMSGTYHYAIYSGKILRLAI
                     CTLAIMWCVSISIGYNTPALFLGGYWAFAALVFNMTTDAKSMHPSNYIVLILIASIFI
                     YVRLHFIYRDGVSSKLTYQLGELVKGASGIYTNKNTYDLIAEIAKIKKQQADKAIFLP
                     DFTALQITTSHLSKIKTEWPNKTEIPNEQILNKVASAIDFNDTSTYYYIAKYQTAQLA
                     DTLIPLVNNGLDYRIIRYVKEHTSKQGETRFFEIYHK"
     gene            complement(4345..6144)
                     /locus_tag="PLF48_06805"
     CDS             complement(4345..6144)
                     /locus_tag="PLF48_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF49068.1"
                     /translation="MESTETTVKNLLKGGEFLIKDMPFSDTFTPEDLNEEQKMIIDTT
                     VSFIDTEVLPVYDRIEKQEPGLTESLLEKAGELGLLGLAIPEEYGGMGKDFNTNSLLV
                     EYMAKSRSFSLSLGAHIGIGTLPIVYFGNEAQKAHYLPKLASGELKACYCLTEPGSGS
                     DALGAKTTAILNPEGTHYIVNGQKMWITNSGFADVFIVFVQVGGDKFSGLIMEKGMEG
                     LSLGAEEKKMGIKGSSTRQVFFENVKVPVENLLGEVGKGHLIAFNILNVGRYKLAAGV
                     LGASKMVIEQSAQYAVERQQFKKPIATFGAIQYKLAEMTTRTYALQSAVYRAADAIDN
                     KEKELMAEGKSVQEAIMKAAEEYAIECSILKFFGSEVLNYCVDENVQIHGGMGYSEEL
                     GAAPAYRDARINRIFEGTNEINRLLTVDMLMKRAMSGKIDLMTPGMAIQKELMSVPDF
                     SADESNDVLAGELKAIKNLKKAVLLVAGGAAQKLMAKLKDEQEILMNAADMMAELYVI
                     ESSVLRTQKLIADRGEAACALQIDMTKVYTSDALERVFVYGKHALQSFAEGDELRIML
                     MGLKRFTKYEPFNTKDARRRIAAKVLEEGKYCF"
     gene            6342..6980
                     /locus_tag="PLF48_06810"
     CDS             6342..6980
                     /locus_tag="PLF48_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49069.1"
                     /translation="MKVKLFALIFSSFFYNSFYAQVFSIPHTTYINTPYGNIPITTYS
                     YVNFPNFYFRNSGYNPDKKYEFKVTLLNDSVLTFKSAIHLNENEHYLILKNAVLDKRR
                     IYVHDTKEISAQFKEYEFVGTPNDSCWLFRANDDSIYLYSMLPEDEPTYYTYMSRRGV
                     PNSIQAITQENVEQVVKDDKDLLKMSKKKRKLIDVLLKFNKNTTPEETETGK"
     gene            7038..>7442
                     /gene="folE"
                     /locus_tag="PLF48_06815"
     CDS             7038..>7442
                     /gene="folE"
                     /locus_tag="PLF48_06815"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HNF49070.1"
                     /translation="MNDLITNAHLGIEPNQEMQQHYKEIIRLLGENPEREGLVKTPYR
                     VSKAMQFVTHGYSLNPVEIIQSAIFNEDHKEMVIVKDIELYSMCEHHMLPFFGKAHVA
                     YIPDGKITGLSKIARVVDAYARRLQVQERLTMQ"
BASE COUNT         2420 a         1318 c         1325 g         2379 t
ORIGIN      
        1 atattcagca tacgtaattt tttgatagct ggttttatgg acttatgatt atctttgttt
       61 catgttagat tatggaaaat acgaagccgt tattggtctg gaagtacatg cacaattaaa
      121 tacgctaact aaaatatttg ctgcagatga aactgcgttc ggtgctttac ccaatacgca
      181 tatcagccca attacattag gaatgccggg tgtattgcct aaactaaata aagaggtggt
      241 agataaagca ataaaattcg gcttggcttg taatagtgaa atttcgcaac agactttttt
      301 tgcccgaaaa aattatttct atgccgattt acccaaaggc tatcagattt cgcaagataa
      361 taatccgata tgcgttggag gatttatcga aataaacgat acgggagtaa agaaaaaaat
      421 aagattgcat catgcacaca tggaagaaga tgccgggaaa aataatcatg agttacatgc
      481 cgatttttct cttatagatt taaaccgtgc aggtgttcca cttttggaaa ttgtatcaga
      541 accggatatg catactcccg acgaggctta tgcttatttg catgaaatac gaaaattagt
      601 acgctatatt gatatatgcg atggcaatat ggaagaaggc tctctgcgtt gtgatgccaa
      661 tatttcaata agacttaaag gagaaagcat actcaataca cgggtagaag tgaagaatat
      721 gaattctatc cgccacgtaa aaaaggctat tgaaaatgaa ataaaacgcc aaatagattt
      781 ggtagaaagt gggggagaag tagtgcagga aacccggagt tttcatgccg aagacggttc
      841 tagttttgtt ttgcgcagta aagaaaatgc gcatgattat cgctattttc cggaacccga
      901 tttgccgcca gtccttattt caaatgaaaa actggaacag ttgaaatcaa ccatgccacc
      961 attgccaaat gctttgtttt tgcagtttac acaagcatat catttatctg aacaagatgc
     1021 ggttatgctg actgaagaca gagatactgc ttatttcttt ctggatatga taaggcattc
     1081 ttcaaattat aaagcggttt ctaactggct caacggcgaa ataaaatctt atctgaatga
     1141 taatcattta aagattacgg acttaaaact ccgtccggag caattaggcg aaattattat
     1201 gcttttggat gaaaataaga taaattcaac aggagcaaaa accatttttt atcacttatt
     1261 ggaaaacacc aatcaaacag ccctgcaagc tgctgaatca ttaaacttga tgcagtcatc
     1321 agataaggat gagcttacgc tctgggctac acaagtattg gaaagcatgc cggataaagt
     1381 agcggaatat aaaaaaggta aaaaaggaac gataaatctc tttgtaggca atgtgatgaa
     1441 attatctaaa ggaaaagcag caccgaaaca aattacacaa attttagaag acttattaag
     1501 caaataaaaa ccatgaaaag aattaattat atcctatcta tttgccttat tttattgctt
     1561 attccttcat gtaaatattt caacgggtat caaattgatg gaaaaattca acatgcaaac
     1621 ggagaaaaag tttatctgga agatattttt caaacacctg ccttgactat agatacggca
     1681 actatttccg gtagtacttt tcatcttaaa aactacagtg ataaaggcat ttatcgttta
     1741 agatttggcg ataacccgga taatagtttg ttcatatatt tagacaaaaa acaaaaacta
     1801 agcatatcag cagattttaa acaactatac aattatacca taagtggaaa caaagcatcg
     1861 caaacaattg ctcaattact aaataccaat tatcagaact tggtacaaat tgatagcgcc
     1921 aataacgaat acatgcgggc aggtgctaaa caaaaagatt ctatgctggg aattttgaaa
     1981 aataaaaaat cggcatactt agattattta cacaacttca ttaaaaaaga agataatgcg
     2041 gatgttgctt gttttgccct gcaattactg gagccatata aagaagaaga aattccattt
     2101 attattgaac aagtaaaaaa tcttcatgaa tctgctccaa actcgcgaca aatcaatttg
     2161 tggtatgctg aagtgcaaca atatgaaaaa caacttttgg caaaacaaga agaagggtta
     2221 cctgttggag aaattgcacc aaatattatt ttacaaaatc cccaaggaga tactattcaa
     2281 ttaaaggaat tgaaaggaaa atatgtattg ttggattttt gggcaagctg gtgtcagccg
     2341 tgccgtcaaa ataatccgca tttggtacag atttttgaaa aatatcattt tcaaggattg
     2401 gagattttct cagtttcact ggatgatacc aaagaacgct ggatgaatgc catcaaagtt
     2461 gataaattaa aatggcagta tcatggttgt gattatcaaa aatggaatgg aaaagccgtg
     2521 aatatgtata aagtatcttc cataccagct gtgtttttgt tagataaaaa tggaaaaatc
     2581 atcgctaaaa atttacacgg tgcagcattg gatgcgaaat taaacgagct tttttctact
     2641 gcctcagcta aatgaaaaaa caaacaactc tcgatatcgc ttttattttc ggcttgagtt
     2701 tatgctgtca ttttttattt tctaaatacg gattcaatcc cacagatgaa ggttttgtgc
     2761 tttcatcatc caacagaatt ttacacggac aaattccgca tatagatttc tcatctttac
     2821 gacctctagg atatgcttat ttgcatattc cggaattact gatttctaaa agatacatgt
     2881 ttttagtttc tcgatatata ttttggctgg aacaggtatt gattgcattt ttatgggtac
     2941 ggttagtaca acaatatctt ggagtattcc aaaatgctgt ttttcagtac atactcatgt
     3001 tcacgtgttt ggtgtttaat gtgcactatt ttccagttgc tgtgctacat acgatagacg
     3061 gacttctttt tgcagttgca ggactattct ttttgcaaaa aaaatcggtg atactgaatt
     3121 tgattggtgc actgtttttc ggtttcagcg taatttgcaa acaaggcttt attgttatga
     3181 ttccggttgc ttttattttg ttttggaata agcgtatcac cttgttactg tcctttttgc
     3241 ctgtattggt ttatgttatt ttgctctata tgaaaggagg cgcaacagat ttgaagatgc
     3301 agcttaccgg gcatacagaa atagtggata ttgggtttaa gtcctacttt ttcaatcttt
     3361 atttttggtt gggagtaaca acagtaataa ttgtcagaag gtttaaattt tttcacatct
     3421 attggttttt tcaactactg ctcctgtgtt ttttattagt tcgatttcat tatcacggaa
     3481 agttgttgtt tttttacagt ggtttgctta ccgcggaaat aatgattgct ttaatcaaca
     3541 tcaaaatacc tctttattct atggaaccgg ataaagttta ttttgaaaac gaagaaatga
     3601 gcgggacata tcattatgcg atttattcag gtaaaatact tagacttgct atctgtacct
     3661 tagctatcat gtggtgtgta tcgatatcga taggatataa tacaccggca cttttcctgg
     3721 gtggatattg ggcatttgct gcacttgttt ttaacatgac gacagatgcc aaaagcatgc
     3781 atccgtcaaa ctatatagtg cttatcttaa ttgccagtat ctttatttat gtcagattgc
     3841 attttatata tcgggatggt gttagctcta aattgactta tcaacttggt gaacttgtaa
     3901 aaggtgcatc tggaatttat accaacaaaa acacgtatga tttaatagca gaaattgcaa
     3961 aaatcaaaaa acaacaagct gataaagcca tatttttacc tgatttcact gcacttcaaa
     4021 ttacaacatc acacctttct aaaataaaaa cagaatggcc aaataaaact gaaattccca
     4081 atgaacagat tttaaacaaa gtagcttcag caattgattt taatgatact tccacttact
     4141 attatattgc aaaataccaa actgcacagt tagctgatac gcttattcca ttagttaata
     4201 atggtctgga ttatcgtata atacgctatg taaaagaaca tacttccaaa caaggagaaa
     4261 cccggttttt tgaaatttat cacaagtaaa ttataaaaaa agaggctgaa ttttcagcct
     4321 tttttctata cagattacta tagcttagaa acaatatttt ccttcttcca aaacttttgc
     4381 cgcgatacgt ctgcgtgcat cttttgtgtt gaatggttca tatttagtga atcgttttaa
     4441 acccatcaac atgatacgca gttcatcacc ctctgcaaaa gattgtaacg cgtgtttgcc
     4501 atatacaaaa actctctcca aagcgtctga cgtgtatact tttgtcatgt caatttgcaa
     4561 agcacaggct gcttctccgc gatctgcaat tagtttttga gtacgcaata ctgacgattc
     4621 tataacataa agctcagcca tcatatcagc agcattcatc aaaatttctt gttcatcttt
     4681 tagttttgcc attaattttt gagcagctcc acctgcaaca agcaatactg cttttttcag
     4741 gtttttaatg gctttgagtt caccagccaa aacatcgttt gattcgtcag cactaaaatc
     4801 aggcacactc atcaattctt tctgaatcgc cattccgggt gtcatcaaat ctattttacc
     4861 tgacatcgct cgcttcatca gcatatctac agtaagtaat ctgtttattt cgtttgtgcc
     4921 ttcaaaaatt cgattgatac gtgcatctcg gtatgccgga gcagcaccca attcttccga
     4981 gtagcccatt ccgccatgta tctgcacgtt ttcatctacg caataattca atacttcaga
     5041 accgaaaaat ttcaaaatag agcactcgat tgcatactct tcggccgctt tcatgatagc
     5101 ttcctgaaca gattttcctt ctgccattaa ttctttttct ttattgtcaa tagcatcagc
     5161 tgcccgataa accgcacttt gcaatgcgta agtacgagtt gtcatttctg ccagtttgta
     5221 ttgtattgca ccaaaagtgg caattggttt tttaaattgc tgacgctcta ccgcgtattg
     5281 ggcagattgc tcgatgacca ttttagatgc gcccaaaaca cctgctgcta atttatatcg
     5341 tcctacgttt aagatgttaa aagcaattag atgtcctttg ccaacctcgc ctaataagtt
     5401 ctcaaccgga actttcacgt tttcaaaaaa tacctgacgg gtagacgaac ctttgatacc
     5461 cattttcttt tcttcagctc caaggctcaa gccttccata cctttttcca tgattaagcc
     5521 ggaaaattta tcgccaccca cttgaacaaa cacgataaat acatctgcaa agcccgagtt
     5581 ggtaatccac attttttgac cattcacgat gtaatgcgtg ccttccgggt ttaaaatcgc
     5641 tgtagtttta gctcctaatg catccgatcc cgatcccggt tctgtcaagc aatagcaggc
     5701 ttttaattct ccgcttgcta acttgggtaa gtaatgtgct ttttgggctt cattgccaaa
     5761 atatacaata ggtaaggtgc caatgccaat atgcgcacct aaagaaagag aaaatgaacg
     5821 agattttgcc atgtactcca ctaacaaaga gttggtatta aaatctttgc ccataccgcc
     5881 atattcttcg gggattgcca agcctaataa gcccaattct ccggcttttt ctaaaagcga
     5941 ttctgttaaa cccggttctt gtttttctat tctatcataa accggcaata cttccgtatc
     6001 aatgaaagat actgttgtat caataatcat tttttgttct tcatttaaat cttccggtgt
     6061 gaatgtatcg gaaaagggca tgtctttaat taaaaattcg ccccctttaa gcaaattttt
     6121 tactgtagtt tctgttgatt ccatgttgat tttgttattt aataatttta ctaagcagtt
     6181 tactgattta aaattacaat gcatgcattg taattacaag atttggctaa ttaagtaaag
     6241 gttaagttag aaactgaaaa aataaaattt tttgttggga gtaggaatga aaaagttact
     6301 agtattattc cggtaatgta tatatttgta aaaaagtttt tatgaaagtt aaattatttg
     6361 ccttgatttt tagttcattt ttttacaata gcttttacgc gcaagttttc tctattccgc
     6421 atacaacgta tatcaatacg ccctacggta atattccgat cacgacctat tcatatgtga
     6481 attttccgaa tttttatttt agaaattccg gttataaccc ggataaaaaa tatgagttta
     6541 aagtaacact cttgaatgat agtgttttaa catttaaatc ggctattcat ttaaacgaaa
     6601 atgagcatta tttgattttg aaaaatgctg ttttagataa aagaagaatt tacgtgcatg
     6661 atacaaaaga aatttcagca caatttaagg aatacgagtt tgtaggcact ccaaatgatt
     6721 cgtgctggct atttagagct aatgatgatt ctatttattt atattctatg ttgccggaag
     6781 acgagcctac gtattatact tacatgagca ggagaggagt acctaactct atacaagcca
     6841 taacacaaga aaatgtggaa caagttgtaa aagatgataa agacttgttg aaaatgtcaa
     6901 agaaaaaacg aaaattgatt gacgtactct tgaagtttaa taaaaatacg actcccgagg
     6961 aaacagaaac aggaaaataa gcatgttgac ggttcgactg tactttacaa tacacaatta
     7021 atgtttaact ttggcaaatg aacgatttga ttacaaacgc gcatttggga atagagccaa
     7081 atcaggaaat gcaacaacat tataaagaaa taattcggtt gttgggcgaa aatcctgaaa
     7141 gagaaggctt ggtaaaaaca ccatacaggg tgagcaaagc catgcagttt gttacacatg
     7201 gctattcgct gaacccggta gaaatcatac aatccgcgat atttaatgaa gatcacaaag
     7261 aaatggtcat cgtgaaagat atcgaactct attcgatgtg cgaacaccat atgttgccgt
     7321 tttttggcaa agcgcatgtg gcctatattc cggacggcaa aataacagga ttgagtaaaa
     7381 tagcccgtgt ggtagatgct tatgctcgcc gtctgcaagt gcaagagcga ttaaccatgc
     7441 aa
//
LOCUS       DAOQII010000116         7377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5544_length_7377_cov_5.909041, whole genome shotgun sequence.
ACCESSION   DAOQII010000116 DAOQII010000000
VERSION     DAOQII010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_7377_cov_5.909041"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLF48_06820"
     CDS             <1..1267
                     /locus_tag="PLF48_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNF49071.1"
                     /translation="NAKTENDTFLRTDKLAKNYLGIINDPKFGFQKANIAVELDRPNL
                     IYGDSLRNFTVDSAYLLLRYYTIYGDTNVAQDIQVSEINTNINPDLLYYSNNTTFSGS
                     TTLGQRNSYKYVPTRKETVSRTDTIGISNALKIPISNSFAQAILDLPDSIQRDTIEFR
                     NRIKGLFIENASLNGNAMAEFDFNSIYSGLYIYFKDKHNNNKDIRLQTYIYGYINGVV
                     SQKKRSVNLYQNTLSPQVQSAIASSAISDSIIYFLGQNGTRVKLSLPTIANLGKQTIN
                     LATIELTQMEDNSIGSYSKPQSLFLLKRNSSGDLAILPTNEGVAIFDTTATDDFGRKI
                     TRYRFNITRYMQTLVSGSGTNSDLYITNYPPAGTEVAYNNLSITPKALASSTDDYFGY
                     APERVILAGPNNSNQKYKLKLKLYYTLLK"
     gene            1325..3172
                     /gene="glmS"
                     /locus_tag="PLF48_06825"
     CDS             1325..3172
                     /gene="glmS"
                     /locus_tag="PLF48_06825"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNF49072.1"
                     /translation="MCGIVGYIGKKEAYPLILNGLKRLEYRGYDSAGVALLSNSGKLN
                     IYKKKGKVSALEQETEGKDISGTVGIGHTRWATHGEANDVNAHPHFSTNGDIALIHNG
                     IIENYAALKTELQSRGYKFRSETDTEVLVHLIEEIQKNVECDLAEAVRLALNEVVGAY
                     AIVVISTREPDKLICAKLSSPMVIGFGDGELYIASDGSPLVEHTKKVSYLEDGQMAVL
                     HSNGNIELKTIIDNITHVPYIDELQLKLEQIEKGGFDHFMMKEIYEQPQAIKNSIRGR
                     IDSEQFWVNLGGVREYEARFKNAERIIIVACGTSWHSALIGEYLIEEFARINVEVEYA
                     SEFRYRNPIITERDVVVAISQSGETADTKAALELAKSKGALTYGICNVVGSNISRMTD
                     AGSYTHAGPEIGVASTKAFTTQVSILALLALQLSEIRGTLSQSQFNTYIAEMEALPEK
                     IERILKANSFIYEIAETFQHANNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAE
                     MKHGPIALIDENMPVVVLAPQSQHYEKVLSNIEEVKARKGRIIAVVTEGDTAISNLAE
                     YVIQIPDTDEPFSPILSVIPLQLLAYHIAVMRGCDVDQPRNLAKSVTVE"
     gene            complement(3177..4616)
                     /locus_tag="PLF48_06830"
     CDS             complement(3177..4616)
                     /locus_tag="PLF48_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49073.1"
                     /translation="MKNLLTTRIISALNQKDILLISDYLKQEKRKSLFKLFQLLLNEK
                     ENIKPDRAKIFQKLFKQKLTKSNDYLLRNELKLLKDKMEDAYIANKTAETMLRQKNNL
                     KLEWYRHLKITDEYTEILNTQYEFNKQHFRLDNTLYNSFIYADFIRINTSNYAERLRL
                     MQENLSHFETTFHQYITEQYSIWCLMKSHVLLQQKQMDNTHKTASFRHETLHIKTEDY
                     RNYLTEYYLAYANAYCNFDTSTIAEWENIYALLLKNPDNEIIESEKCYTLGNLATICS
                     IRNQFEKADFYFSELFNSLPEAITSQNMALTLNYITNLNKLKFHEMARVKMQNAVVVF
                     GNKIKSFSQFRTQQIVAACYLANTKELGSLLAVDFETLQPFERIFYRLFYCIYFIQLE
                     DFELAFTEIQNLQRSKLMKEIDTHYELIADYFYVCIKHGLSVSGNYTKCSSKAIKEIE
                     SANERIIKSGVPILQNYSPYQWMKERVNS"
     gene            4766..5368
                     /locus_tag="PLF48_06835"
     CDS             4766..5368
                     /locus_tag="PLF48_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49074.1"
                     /translation="MKNTFILLLAFMLLATSCKKEKNNLNSTTPVQSFTDVTMGNQSS
                     ASYNCFMSFDSAKVYTLINAQANQTPIDLIFLHNNPDNLAMFVSPASIVDAITLDADI
                     YTNPIYGVENWTTKNAIQIGITDISVAEFNSINTNGNLHAAYENDPHVTIGWEIDIKP
                     NKVYKYTSNRTGKRGLIKVKSINGNYSSAGSILFDLKQIQ"
     gene            5374..5883
                     /locus_tag="PLF48_06840"
     CDS             5374..5883
                     /locus_tag="PLF48_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49075.1"
                     /translation="MTKKVIILLIAIANLTLGCKKDETANENSHPYAGTVRLYETKRN
                     WKLKYHYNGSIAHFSVSIGTEELIELLPGAQKNQYYIRPKDHNEYCLDAQAKDDLFIK
                     LYSYFGNNSQLFTITNIGDNKVRIQSVADTSLYLLNNSDYASFSYSSYLKPRSYDTNA
                     LDFWIIEKL"
     gene            5896..6771
                     /locus_tag="PLF48_06845"
     CDS             5896..6771
                     /locus_tag="PLF48_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49076.1"
                     /translation="MKNSILFASILFVFILTSCTNSNTPTPQPTPTNNSSKTVLYDVN
                     NIVDPNDDDYCFMLLSANNSAQKKYFKAKMYDVLRDSMVITEGPYTLDSLIDVSTFPS
                     YIKSASSGLYLTDFVFSDSVRVRQTGLNANGKRVFTQVPWSGNGANYVKPYTKGAQIE
                     GTAILNYFKKPTATGSFEGYLMHFFFNKGTCIVFSKTSASGTSVSVSGFPEDISSVIG
                     ATYDWQNVDNYFNVTTGIGVSTHYFIDYKNWRYFRIKESKTSVPGTPGGQHAIEYGNY
                     KSLDKLLKWPDDWKK"
     gene            6791..7189
                     /locus_tag="PLF48_06850"
     CDS             6791..7189
                     /locus_tag="PLF48_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49077.1"
                     /translation="MKTITTLLLLFCTSILHAQTIVGKWKCIANYSSFDNKQTNMEDA
                     LHQSRPCTKNTIYEFLSDGKIKRTYSGCDEKYVITQDKLWKKQQWKLEGKTLKTSVTN
                     FMVFREYTLTFSGNKMTWTNKDETIIYEKL"
BASE COUNT         2516 a         1261 c         1335 g         2265 t
ORIGIN      
        1 aaatgcaaaa actgaaaatg atacttttct gcgcaccgac aaattagcca aaaactatct
       61 gggaattatt aacgacccta agtttggttt tcaaaaagca aatattgcgg ttgagctgga
      121 cagacccaat cttatctatg gagattcact gagaaacttc acggttgact cggcttattt
      181 attgttgcgg tactatacta tctatggcga tacaaatgtg gcacaagata tacaagtgag
      241 cgaaatcaat accaatatca atccggactt actctactat tcaaacaata ctactttcag
      301 cggctcgacc actttaggcc aacggaatag ttataaatat gttcctacac gaaaagaaac
      361 tgtatctcgt actgatacta ttggcatcag taatgcactt aagataccaa tcagcaatag
      421 ttttgctcaa gccatattag atttaccgga ctcaatacaa agagatacta tcgaattcag
      481 aaacagaata aaaggattgt ttattgaaaa tgcaagtttg aacggaaatg ccatggcaga
      541 gtttgatttt aacagcatct attctggatt gtatatttat tttaaagaca aacacaataa
      601 caacaaggat atccgtcttc aaacttatat ttatgggtat atcaatggcg ttgtttcgca
      661 gaaaaaaaga agtgtcaatc tttatcaaaa tacactttca ccacaagtgc agtctgctat
      721 cgcatcatcg gcaatcagtg acagtatcat ttatttttta ggacaaaacg gcacaagggt
      781 aaaattaagc ttacccacta ttgctaattt aggtaaacaa acaatcaatc tggcaactat
      841 tgaacttaca caaatggaag acaattcaat aggctcctac agcaagccac aaagtttatt
      901 tttattaaaa agaaacagtt cgggtgattt ggccatcttg ccgacaaatg aaggggttgc
      961 catatttgat actacagcta cagatgattt tggcagaaaa attacacgtt atcggtttaa
     1021 tattacccgt tatatgcaaa cattagtatc tggctccggt actaatagcg atttgtatat
     1081 taccaattat ccgcctgcgg gtacagaagt tgcgtataat aatttaagta ttacccctaa
     1141 agcattggca agttcaacag atgattattt tggctatgca ccggaacgtg tgatattagc
     1201 cggaccaaat aattcaaatc agaaatacaa gctgaaactg aaattgtatt ataccttgtt
     1261 gaaataattt atttcccgta tctttctttg ttgaaaaaca aagaataaac taaactaaac
     1321 gattatgtgt ggaattgtcg gttatatagg caaaaaagaa gcttatccgc ttatccttaa
     1381 tggcttaaaa cgattggaat accgtggcta tgacagtgcc ggagtggcat tgctcagtaa
     1441 ttcaggcaaa cttaatattt acaaaaagaa agggaaagtt tctgctttag aacaggaaac
     1501 cgaagggaaa gatatttccg gaacggtagg tatcgggcat acccgttggg ctactcatgg
     1561 cgaagccaat gatgtgaatg ctcatccgca tttcagcaca aatggcgata ttgctttgat
     1621 acataatggt atcattgaaa attatgccgc attaaaaaca gagttacaat cgcgcgggta
     1681 taaatttcgt tctgaaactg acaccgaagt attggtgcat ctgattgaag agatacaaaa
     1741 aaacgtagag tgcgatttgg ccgaagctgt gcgattagcg ctgaatgaag ttgttggtgc
     1801 ttatgcaatt gttgtcatct cgaccagaga accggataaa cttatttgcg caaaattaag
     1861 ctcgccaatg gtgattggtt ttggcgatgg cgagttgtat attgcctctg atggctctcc
     1921 acttgttgaa catactaaga aagtatcgta tctcgaagac ggacaaatgg cagtattgca
     1981 cagcaatggc aatattgaac tcaaaacgat catagacaac attacccatg tgccgtatat
     2041 cgatgagctc cagctcaaat tagaacaaat tgagaaaggc ggtttcgatc actttatgat
     2101 gaaagaaatt tatgagcaac cacaagccat aaaaaacagc attcgaggca ggattgacag
     2161 cgaacaattt tgggtaaatc ttggcggtgt aagagagtac gaggcacgtt tcaaaaatgc
     2221 ggagcgcatc attattgttg cttgtggcac ttcctggcat tctgctttaa tcggggagta
     2281 tcttattgaa gaatttgccc gtataaatgt tgaagtagaa tatgcttctg agttcagata
     2341 ccgcaatcct attattactg agcgtgatgt ggtggttgcc atttctcagt caggcgaaac
     2401 agctgataca aaagcagctt tagagttagc caaatcaaaa ggtgcgctga cctatggtat
     2461 atgtaatgtt gtaggatcta atatatcacg gatgacagat gccggctctt atacacatgc
     2521 cggtccggaa ataggtgtag cttctaccaa agcatttacc acacaagtga gcatactcgc
     2581 cctattagct ttgcagttga gcgaaatcag aggcacgctg tctcaatctc agtttaatac
     2641 ctatatagcc gagatggaag ctttacccga aaaaatagaa cgtattttaa aagcaaattc
     2701 ttttatctat gagattgcgg aaacctttca acacgccaac aatttcttat acctcggcag
     2761 agggtataat ttcccggtag cattggaagg ggcacttaaa ctcaaagaaa tttcttatat
     2821 ccatgcagaa ggctacccgg ctgccgaaat gaaacatgga cctattgcac tcatagatga
     2881 aaatatgccg gtggttgttt tggctccaca atcacagcac tacgaaaagg tattgtcgaa
     2941 tattgaagaa gtaaaggcta gaaaaggtcg catcattgct gtcgttacag aaggtgatac
     3001 agccatcagc aatctggcag aatatgtgat tcagataccg gatacggacg agccattttc
     3061 acctatttta tcggttattc cattacagtt attagcttac catattgcag taatgcgggg
     3121 atgcgatgta gaccagccaa gaaacctggc gaaaagtgtg acagtggaat aataaattaa
     3181 gaatttactc tctctttcat ccactgatat ggagaataat tttgtaatat aggtacacct
     3241 gattttatga ttctctcatt tgcagattca atttctttaa tagctttgct ggaacattta
     3301 gtatagttgc cacttacaga caagccgtgt tttatacaaa cgtaaaaata atctgctatt
     3361 agttcatagt gtgtgtctat ttctttcatt agtttagatc gctgtaggtt ctgaatttct
     3421 gtaaatgcca attcaaaatc ttcaagttga atgaagtaaa tacaataaaa aagccgatag
     3481 aaaatgcgct caaatggttg caacgtttca aaatctacgg caagcaaact gcctaattct
     3541 ttagtgttag caagataaca agcagcgact atctgttgtg ttcggaattg actgaaggat
     3601 tttattttgt tgccaaacac cacaacagca ttctgcattt taactcgtgc catttcatga
     3661 aatttgagtt tattgagatt ggtaatataa ttgagtgtga gtgccatatt ttgagaagtt
     3721 atagcctcgg gcaacgaatt gaacaattca gaaaaataaa aatctgcttt ttcaaattga
     3781 tttcgaatag agcaaatggt ggcaagattg cccaatgtat agcacttttc cgattctatt
     3841 atctcattat ccggattctt taaaagcaaa gcatatatat tttcccattc tgctattgtt
     3901 gatgtatcaa aattgcaata cgcattggca taagctaaat aatattcggt aagataattg
     3961 cgataatctt cggtcttaat atgcaaagtt tcatgcctga atgatgccgt tttatgcgta
     4021 ttatccattt gtttttgctg taataaaaca tgtgatttca tcaggcacca gatgctgtat
     4081 tgctctgtaa tatattgatg aaacgtggtt tcaaaatggg ataaattttc ttgcataaga
     4141 cgaaggcgct ccgcataatt tgaggtattg atgcggataa aatcagcata gataaagctg
     4201 ttgtacaatg tattatcaag tctgaaatgc tgtttgttga actcgtattg agtatttaaa
     4261 atttcggtat attcatctgt gatttttaaa tgacgatacc attctaattt caggttgttt
     4321 ttttggcgca acatcgtttc agcggttttg tttgctatat aagcatcttc cattttatct
     4381 tttaataatt tcagttcatt tcgcaataga taatcgtttg atttggtgag tttttgttta
     4441 aataatttct gaaagatttt tgctcgatcc ggtttaatat tttctttttc gttgagtagt
     4501 agctggaata atttgaataa agattttcgt ttttcctgct tgagatagtc tgaaataaga
     4561 agaatgtctt tttggttaag tgcagaaatt atgcgtgtag tgagcaaatt tttcattaca
     4621 gaaaataatg tttaagtgtt tgattgataa atatttatgg tttatttata atgtaaaact
     4681 cattcatttt tcattacaaa aataaagaaa ttgtttttat gttttagagt tacttaaata
     4741 aatttgaatt ctaaaactat attacatgaa aaacacattt atcttacttc ttgcatttat
     4801 gctgttggca acttcttgta aaaaggaaaa aaacaacttg aacagcacta caccggtaca
     4861 aagttttacg gatgttacaa tgggcaatca atccagcgcc agttacaact gcttcatgtc
     4921 gtttgattcg gcaaaggtat acaccctcat caatgcacaa gcgaatcaaa ccccgataga
     4981 cctcatcttt ttacataaca atcctgataa tttagccatg tttgtttcgc ctgcttctat
     5041 agttgatgcc ataactttag atgcagatat ttataccaat ccgatatatg gggttgaaaa
     5101 ctggacaaca aaaaatgcaa tacaaatagg cattacagat atctcagttg ccgaatttaa
     5161 tagtataaat acaaatggaa atttacatgc agcatacgag aacgacccgc atgttaccat
     5221 tggatgggaa attgacataa agcccaataa ggtttacaaa tacacttcaa acagaacagg
     5281 aaaacgggga ttaattaaag taaaatccat aaacggaaat tacagttccg caggcagtat
     5341 tcttttcgat ttaaaacaaa ttcaataact atcatgacaa aaaaagtaat catcttgctt
     5401 attgcaatag ccaaccttac tctaggttgc aaaaaagacg aaacagccaa tgaaaattca
     5461 catccttacg caggaacggt aagactgtat gaaaccaaaa gaaattggaa attaaaatac
     5521 cattataatg gaagtattgc acatttttct gtaagcattg gcacagaaga attaatagaa
     5581 ttacttcctg gtgctcaaaa aaatcaatat tatatcagac caaaagacca caatgaatat
     5641 tgtttagatg ctcaagctaa agatgattta tttataaaat tatatagcta ttttggcaat
     5701 aatagtcagc tatttactat cacaaatatt ggtgacaata aagttagaat tcaatctgta
     5761 gcagatactt ctttatatct actaaataat tctgattatg cttcgttctc ttatagttca
     5821 tacttaaaac ctagaagtta tgatactaat gcacttgatt tttggataat tgaaaaactg
     5881 taaattttat aaactatgaa aaattctatc ttatttgcat ctattttatt tgtattcatt
     5941 ttaacaagtt gcacaaacag caatacccct acaccacaac caactccaac caataattca
     6001 tcaaaaacgg tgctatatga tgtaaataat attgtagatc ctaacgatga cgactattgc
     6061 tttatgctct taagtgcaaa caattcggca cagaaaaagt actttaaagc aaaaatgtac
     6121 gatgtactac gtgattctat ggttattacg gaaggtcctt atactttaga tagtttaata
     6181 gatgtaagta catttccatc ttacataaaa tctgcaagtt ccggcctgta cttaacagat
     6241 tttgtttttt ccgatagtgt ccgtgtacga caaacaggat taaatgctaa tggaaaaaga
     6301 gtatttacac aagtaccttg gtcgggtaat ggcgctaact atgtaaaacc atataccaaa
     6361 ggtgcccaaa tagaaggaac agcgattcta aattatttta aaaaacctac cgctacaggt
     6421 agttttgaag gatacttgat gcatttcttt tttaataaag gcacttgtat tgttttctca
     6481 aaaacaagtg catctggtac aagtgtttct gtatcgggtt ttccggaaga tatttcatcc
     6541 gtaattggtg caacttacga ttggcaaaac gtagataatt atttcaatgt aactactgga
     6601 ataggtgtct ctacccatta ttttattgat tataaaaatt ggcgttattt tagaataaaa
     6661 gaaagtaaaa ctagtgtacc aggaacacca ggcggacaac atgccataga atatggaaac
     6721 tacaaaagtt tagataaatt attaaaatgg ccagatgact ggaaaaaata aattcttaaa
     6781 tccataactc atgaaaacaa tcacaactct tctcttactg ttttgcacaa gcatattgca
     6841 cgcacaaact attgtaggca aatggaaatg tattgcaaat tattcttctt tcgacaataa
     6901 acaaacgaat atggaggatg ccttgcatca atcgaggcct tgtacaaaaa acaccatata
     6961 tgaattttta tctgacggca agataaagag aacatattcg ggatgcgacg aaaaatatgt
     7021 aataacgcaa gataaactat ggaaaaaaca gcaatggaaa ctggaaggaa agactttaaa
     7081 aacaagcgtt acaaatttta tggtcttcag agaatatacc ctgacattct caggcaataa
     7141 aatgacctgg acaaataaag atgaaacaat tatttatgaa aaattataac tttataaaaa
     7201 aacaaaaatc atgaaaagtt atttttattt aattatctca ctaattatta ttagtgcttg
     7261 tgtgcctacc ggcggaacac ctacacctgc aaatgcaacg attaccgtaa acgggaaaca
     7321 tattattgcg ccttgtggcg atactatagt acctaaagga gtcaactatg ccgtgta
//
LOCUS       DAOQII010000117         7347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5580_length_7347_cov_4.963659, whole genome shotgun sequence.
ACCESSION   DAOQII010000117 DAOQII010000000
VERSION     DAOQII010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5580_length_7347_cov_4.963659"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /gene="dnaK"
                     /locus_tag="PLF48_06855"
     CDS             complement(<1..1676)
                     /gene="dnaK"
                     /locus_tag="PLF48_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNF49078.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGSEPVVITTDEGRRTLPSIVGFLD
                     NGERKVGDPAKRQAITNPKNTVSSIKRFMGKRFSEITKEKSYVAYDVERGDNDTVRVK
                     IHDRLYTPQEISAIILQKCKKMAEDYLGQTVNEAVITVPAYFNDAERQATKEAGLIAG
                     LDVKRIINEPTAAALAYGLDKGGKDMKIAVYDLGGGTFDISVLELGDGVFEVKSTNGD
                     VHLGGDDFDQEIINWLATEFQNEERIDLRKDPMALQRLKEAAEKAKIELSSSTETEIN
                     LPYITAVDGVPKHLVKKLSRAKFDQLTEQLVERTLEPCRKALKDAGMSPSQIDEVILV
                     GGSTRIPKIQEVVEKFFGKKPSKGVNPDEVVAVGAAIQGGVLTGDVKDVLLLDVTPLS
                     LGIETFGGVFTKLIEANTTIPTKKSETFSTASENQPSVELNVLQGERPMAKDNKSIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILSVTAKDKGTGKEQNIRITASSGLSKEEIEK
                     MKAEAKANAAADTEARAKVDKLNEADGLIFQSEKQLKEFGDKLPGDKKQA"
     gene            complement(1838..3394)
                     /locus_tag="PLF48_06860"
     CDS             complement(1838..3394)
                     /locus_tag="PLF48_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF49079.1"
                     /translation="MSNKIINPINQQFLYEFEDPSQAELASVFQRAKDAQAKISKMTL
                     KQRIAEVDKIMQYVIHYQDFILDSIIAETGKSRTDAFAAEVFEVCDVIDVYKKMAPKV
                     LADKKMDIPVFLMGKKARQWAEPLGTILVITPWNFPFYQVIVPGILAFLAGNAVIAKP
                     SEITPLKPLWEHFYAHSGFMKDAIQIVIGGKTTGAELLAFHPDKVHFTGSVRTGKKIM
                     EACSKQLIPVDLELGGKDPSIVFDDIDLDRTVNGVMWGAFTNAGQNCTGIERLYVQEG
                     IYDKFMNDLVDKTKKLRVSTPDRNTKSAEDCDVGCMTATFQVAIVEQHIEDALAKGAK
                     LLCGGIQKKGSMHIPPTIIADCTKDMLIASEETFGPTIAVMKFKTEEEAISLANDSPY
                     GLGASVWTKDLARGERVARAIKCGNVCINNHMINEANPYLPFGGVKDSGIGRFKGEEG
                     LLTFCNLKSVIIDNMSDMIDPHWYPYTETKATLLKNLMDSWFGKRRKYGKFAVNGLKI
                     DSIGKKEKLK"
     gene            3577..5199
                     /locus_tag="PLF48_06865"
     CDS             3577..5199
                     /locus_tag="PLF48_06865"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNF49080.1"
                     /translation="MSNNPDFNLNEDNMRLAVDQLKNKLAKVYFGGGEKKIASEHKKG
                     KLTARERIDYLLDKEITRVEIGALTGDDMYLEEGGCPSGGTVIMIGYVSGRQCIVVAN
                     DATVKAGAWFPITGKKNLRAQEIAMENHLPIIYLVDSAGVYLPMQDEIFPDKENFGRI
                     FRNNAKMSAMGIPQIAAIMGSCVAGGAYLPIMSDESLIVEGTGSIFLAGPYLVKAAIG
                     ENVDKETLGGAETQTALSGVCDYKMKDDKECLDTIKKLVKNFGHFDNAGFDRIKAKKP
                     KAVEKEIYGIFGSKPYDVREIIKRLADDSEFTEYKEDYGQTIVCAYARIDGWSVGIVA
                     NQRLVVKNKKGEVQIGGVIYSDSADKAARFIMLCNQKKIPLVFMHDVTGFMVGSRSEH
                     GGIIKDGAKMVNAVANSTVPKFAIVLGNSYGAGNYAMCGKAYDPRLMLCWPTAKIAVM
                     GGSQAANTILQIQVSAMEAKGKSLTDEEKTAILSEIKEKYEETTTPYHAAARLWVDAI
                     IDPLETRKWISAGIEMANNAPVEKFNVGVIQT"
     gene            complement(5512..>5712)
                     /locus_tag="PLF48_06870"
     CDS             complement(5512..>5712)
                     /locus_tag="PLF48_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49081.1"
                     /translation="FIMGFLARASLEMTWFIFSYRLVSPPTETKANKYRHLERLQMQS
                     GQVVRGLKKKTDNDLFSLSHIF"
     assembly_gap    5713..5812
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5960..6616)
                     /locus_tag="PLF48_06875"
     CDS             complement(5960..6616)
                     /locus_tag="PLF48_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49082.1"
                     /translation="MDNNKNKLLIFVMLFSIWTLQLKADSIETSIITKTYKPKIEYRT
                     NNYDFIVKYKKLKKTDLIAVEILVVCKQQPIGYEDMYVEDYYCKKKMDSYIYYFNDEL
                     AEYAHQIYKIKTNDTLKYSFTIPGCIYTYSITMKIFENYEALKNKGFVSKYIPEYPKL
                     EWITLTDESFNIHNGFKGIDNSMITFSGYRGDYYKSKKKRYKITITKKTNIVTNTNSE
                     "
     gene            complement(6698..7303)
                     /locus_tag="PLF48_06880"
     CDS             complement(6698..7303)
                     /locus_tag="PLF48_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49083.1"
                     /translation="MKLKYLIIYSFLFILLGCSRKTFKFKSGNSYKSAFIKSDSCKSS
                     KSIDIFLKTNVVGSKNLAIVLNNGLSCNYLCGYILTKGKRLFYINWDNYQKGNHKAKE
                     LLYNLNDKKKQAHFIKEFSFIKNCKLQLKSKNFVNKEILSIYSCLHSDTLFDDYIVNE
                     ITFSNINLINSIVLYNFKKGCLVKLLFEEGKCEFEYNGNDF"
BASE COUNT         2096 a         1376 c         1331 g         2444 t
ORIGIN      
        1 atggcttgtt ttttatcgcc cggcaattta tcaccaaact ctttcaattg tttttcagac
       61 tggaagatta agccatctgc ctcattcagt ttgtctactt tagctcttgc ttcagtatcg
      121 gctgcagcat tagctttagc ttctgctttc attttttcaa tttcttcttt gcttaagccc
      181 gaagatgccg tgatgcggat attttgctcc ttgccagttc ctttatcttt agccgttaca
      241 ctcaaaatac cattagcatc gatatcaaaa gtaacttcaa tttgaggtac acctcttggt
      301 gcaggtggaa taccatctaa gtggaagcga ccaatggatt tgttgtcttt tgccatcggg
      361 cgttcacctt gcaatacgtt taattcaaca gaaggctggt tttcgcttgc tgtggagaat
      421 gtttctgatt ttttagtcgg tatggttgta ttcgcttcaa tcaattttgt gaatacaccg
      481 ccaaaagttt ctatacctaa agaaagtggt gtaacatcca acaataaaac atcctttaca
      541 tcacctgtca aaacgccacc ttgtatggcc gcacctactg ccactacttc atccggattt
      601 acccctttac ttggtttttt gccgaaaaac ttctctacca cttcttgaat tttcgggata
      661 cgggtagagc cgcccactaa aattacttcg tcaatttgtg aaggcgacat acctgcatct
      721 ttaagtgctt ttctgcatgg ctctaatgtg cgctcaacta attgctctgt aagctgatcg
      781 aattttgcac gagataattt tttcactaag tgtttaggca caccatcaac ggcagtgata
      841 tatggcaaat tgatttctgt ttctgtagaa gaagacaatt caatttttgc tttctcagca
      901 gcttctttta agcgttgtaa ggccattggg tctttgcgca agtcaattct ttcttcattt
      961 tgaaattcag tggctaacca gttgataatt tcctgatcga aatcatcacc acctagatgc
     1021 acatcaccgt tagttgattt aacttcaaat acaccatcac ccaactctaa tacggagata
     1081 tcgaatgtac caccgcccaa gtcatacacg gcaattttca tatcttttcc gcctttatct
     1141 aagccataag ccagcgcagc agctgtcggt tcgttgatga tacgttttac atccaatcct
     1201 gcaattaatc ctgcttcttt agtagcctga cgttctgcat cgttaaaata tgccggcacg
     1261 gtaataacag cttcatttac tgtttgacct aaatagtctt ctgccatttt tttacatttt
     1321 tgcagaatta tggcagaaat ttcctgtgga gtgtataatc tgtcatgaat tttaacacgt
     1381 accgtatcgt tgtcacctcg ttcaacatca tatgccacat acgatttttc ttttgtaatt
     1441 tctgaaaagc gtttacccat aaaacgcttg atagatgaaa cggtattttt ggggttagta
     1501 atcgcctgac gcttagcagg atctcccact ttacgctcgc cgttatccaa aaaaccaaca
     1561 atagacggaa gagtgcgtct gccctcatcc gtagtaatca ctaccggttc actgccttcc
     1621 attacggcaa cacaggagtt ggtagtccct aagtcaattc caataatttt tcccatatta
     1681 tacttattat ttttttgttt taaaacctct taagaacaat atatatgcca atggcaggca
     1741 agtgacagaa tgacatatta cgaaaataat tgtagaaaat atgggctcga aaggttgcta
     1801 ttcccgaagt aaaatattca ctttaatgaa tcagttatta cttaagcttt tcttttttac
     1861 ctatcgaatc tatttttaat ccattgacag caaacttgcc atattttctt cttttgccaa
     1921 accagctatc catcagattt ttgagtagag ttgctttagt ttcggtgtaa ggataccagt
     1981 gcggatctat catatcgctc atgttgtcaa taatgacaga tttcaaatta caaaaagtga
     2041 gtagcccttc ttcgccttta aatctgccaa tacctgaatc tttgacacct ccaaacggca
     2101 agtaaggatt ggcttcgtta atcatgtggt tgttgatgca tacgtttccg catttgatgg
     2161 ctcgcgctac gcgctcgccc cttgccaaat ctttcgtcca tacggatgca cccagaccat
     2221 atggagaatc gtttgccagc gagattgctt cttcttctgt tttaaatttc atgacagcta
     2281 ttgttggccc aaatgtttct tcagacgcga ttagcatatc tttggtacaa tctgcaatta
     2341 tagtgggcgg gatatgcata cttccttttt tttgtatacc accgcataac aacttcgctc
     2401 cttttgccaa tgcatcttca atatgctgct ctacaattgc tacctgaaaa gtagcagtca
     2461 tacaacctac atcacaatct tcggcagatt ttgtattcct gtctggagtt gaaacacgca
     2521 atttttttgt cttatctacc agatcattca tgaatttatc atatatacct tcttgcacat
     2581 acagtctttc aattccggta cagttttgtc cggcattagt aaacgcaccc cacattacac
     2641 catttacggt tctatctaaa tctatatcat caaatacgat ggaaggatct ttaccaccca
     2701 actccaaatc taccgggata agttgtttgg aacaggcttc cataattttt ttgcctgtac
     2761 gcacggagcc tgtaaaatgt actttatccg ggtgaaatgc cagtaattcc gctccagtgg
     2821 ttttaccacc tattactatc tgtatggcat ctttcataaa tccgctgtgc gcataaaaat
     2881 gttcccacag tggctttagc ggtgtaattt ccgatggttt tgcgattacg gcattcccgg
     2941 ctaaaaaggc caggataccc ggcacgatta cctgataaaa cgggaaattc cagggtgtga
     3001 ttaccaaaat tgttcccaat ggttctgccc attgacgcgc ttttttaccc attaaaaaaa
     3061 caggaatatc cattttttta tcggctaaaa cttttggagc cattttttta taaacatcaa
     3121 tcacatcaca aacctcaaat acttcggcag caaatgcatc tgttctgctt ttgcccgttt
     3181 ctgcaatgat actatccaga ataaaatcct gataatggat gacatattgc attattttat
     3241 ctacttcggc aatacgctgc ttcaatgtca tttttgatat tttagcttgt gcatctttgg
     3301 ctctctgaaa aacagaagct aattctgcct ggctcgggtc ttcaaattcg tataaaaatt
     3361 gttgattaat cgggttgatt attttgttgg acatactatg attttaatgt cctaaaatac
     3421 caacttatga tgagacttaa aaatttacga gcatatatta ttccgagttc gcataatgaa
     3481 ccagcgtgtt gtttataaaa attcttattt tcgagtatat cattatttat gacccgccat
     3541 taaacaagat tacacttgga ttgctaactt tgcagcatga gtaataatcc tgattttaac
     3601 ctgaatgaag acaatatgcg cttagctgtt gaccaactga aaaacaaact ggctaaagtg
     3661 tattttggag gaggtgagaa aaaaattgca tcggagcaca agaaaggcaa actgaccgcc
     3721 cgtgaacgaa ttgattattt attggataaa gaaattacgc gtgtagaaat tggagcactt
     3781 accggagatg acatgtacct cgaagaaggc ggatgcccta gtggtggtac tgtcattatg
     3841 attggctatg tatcaggacg acaatgtatt gttgttgcca atgatgccac ggtgaaagcc
     3901 ggggcttggt ttcctattac cggcaaaaaa aatctgaggg cacaggagat tgccatggaa
     3961 aatcatcttc caattatcta cctggtagac agtgcaggcg tttacttacc catgcaggat
     4021 gaaattttcc cggataaaga aaactttggt cgcattttta ggaataatgc caaaatgagt
     4081 gccatgggta tccctcaaat tgcagccatt atgggcagtt gtgtggcggg tggtgcatat
     4141 ttgcctatca tgagtgatga gagtctgata gtggaaggta cgggcagtat atttttagcc
     4201 ggtccgtatt tagtaaaagc tgctattggt gaaaatgtag ataaagaaac tttgggtgga
     4261 gctgaaacgc aaaccgcact aagcggagtt tgcgactata agatgaaaga cgacaaagaa
     4321 tgtttggata ccataaaaaa actggtgaag aatttcgggc attttgataa cgcaggattt
     4381 gaccgcataa aagctaaaaa gccgaaagct gtagagaaag aaatatatgg cattttcggc
     4441 agcaagcctt atgatgttcg tgaaataata aagcgacttg ctgatgattc cgaatttacg
     4501 gaatacaaag aagactacgg acaaactatt gtatgtgcgt atgcccgtat agatggttgg
     4561 agtgtgggta ttgtagccaa ccagcgattg gtagtaaaaa ataaaaaagg tgaagtgcag
     4621 attggcggtg ttatttattc tgactcggcg gataaggctg cacgttttat tatgctttgc
     4681 aatcagaaaa agattccatt ggtatttatg catgatgtta ccggttttat ggtaggtagc
     4741 cgcagcgagc acgggggcat tattaaagac ggtgctaaaa tggtgaatgc tgttgccaat
     4801 tctacggtgc cgaagtttgc cattgtattg ggcaactctt atggtgcggg caattatgcg
     4861 atgtgtggca aagcatacga tccgcgcttg atgctctgct ggccgacggc aaaaattgcc
     4921 gtgatgggtg gcagtcaggc ggccaacacg attttgcaga ttcaggtttc ggcgatggaa
     4981 gccaaaggca aatcgctgac tgatgaagag aagactgcta ttttatctga aataaaagaa
     5041 aaatacgaag aaacgactac gccctatcat gcggcagcca ggctgtgggt ggatgccatt
     5101 attgatccgc tggaaacccg aaagtggatt tcagcaggaa tagaaatggc aaataatgct
     5161 ccggtagaaa agtttaatgt aggcgtgata cagacttaat acctgagttc tggataatta
     5221 tttcaattta aaaatagttg atttttatct ataatcacct cctttttatt ccacctcctt
     5281 gtgaagttgg aggaataatg agaaatcaac tatttttttc atcctatttt acatttccca
     5341 aactgagatt aattttattg taatgacagc actactgcat tttttttctg atttgaaatg
     5401 cctttgtttc ggttggtgga gacatcaacc gatagtaaaa tatgaaccac gtcatttcga
     5461 gtgaggcacg agcgagaaat cccattatga atatggatgt tttcaaatct attaaaaaat
     5521 atgggataga gaaaacagat cattatccgt tttctttttg agacctctca ctacctgccc
     5581 gctttgcatt tgcaagcgtt cgaggtgacg gtatttgttt gcctttgttt cggttggtgg
     5641 ggacaccaac cgataggaaa atatgaacca cgtcatttcg agtgaggcac gagcgagaaa
     5701 tcccattatg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntccgtttt
     5821 ctttttgaga cctctcacta cctgcccgct ttgcatttgc aagcgtttga ggtgacggtg
     5881 tatgtaatag gatttgtttg cctttgggtt gcctatgttt cggctgatgg ggacaccatg
     5941 cgataggtaa gagttttatt tactccgaat ttgtattggt tacaatgttg gttttttttg
     6001 taattgtaat tttatatctt tttttcttgc ttttataata atcaccccta tacccggaaa
     6061 atgtaatcat ggagttatca attcctttaa aaccattgtg tatgttaaaa gattcatcag
     6121 ttaatgtaat ccattctaat ttgggatatt cagggatata tttactcaca aatcctttat
     6181 tttttagagc ttcataattt tcaaaaatct tcatagttat agaataggta taaatgcaac
     6241 cgggaattgt aaagctgtat tttaatgtat cgtttgtttt tattttatat atctgatgcg
     6301 catactctgc caactcatca ttgaaataat aaatatacga atccattttt tttttgcaat
     6361 aataatcttc aacatacata tcttcataac cgataggttg ctgtttgcac actactaaaa
     6421 tttcaactgc aattaagtcc gtttttttta actttttata ttttacgata aaatcatagt
     6481 tattggttct gtattcaatt tttggtttat atgttttagt aatgatggat gtttcaatac
     6541 tgtcagcttt taactgcagt gtccatatag agaaaagcat cacaaaaatc agaagcttgt
     6601 ttttgttatt gtccattgtt gtcggttatg ctgtttcggt tggtggggac attaaacgat
     6661 agagaaaatt gtcatattta aatattccat gtttttctta aaaatcattt ccattatatt
     6721 caaactcaca ttttccttct tcaaataaca actttaccaa acaccctttt ttaaaattat
     6781 acaatactat tgagtttatt aaattaatat tgctgaatgt aatttcattt actatataat
     6841 catcaaataa tgtatctgag tgcagacaag aatagattga taatatttct ttgttaacga
     6901 aatttttgct ttttagttgc aacttacaat ttttaataaa actaaattct ttaataaaat
     6961 gtgcctgctt ttttttatcg tttaaattat aaagtaattc ttttgcctta tgatttcctt
     7021 tttgataatt atcccaattt atataaaata gtctttttcc ttttgttaga atatacccac
     7081 ataaataatt acaactaagt ccattattta atactatagc taaattcttg cttcctacaa
     7141 catttgtttt taagaaaata tcaatagatt ttgaagactt gcaactatca gacttaataa
     7201 atgcagattt ataactattg ccggatttaa acttaaatgt tttacgtgaa caacctaaaa
     7261 gtatgaataa gaatgaatat ataattaaat atttaagttt cataactaaa gacaatttaa
     7321 ttcatttact cttaaacatt tttttca
//
LOCUS       DAOQII010000118         7299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5630_length_7299_cov_4.792518, whole genome shotgun sequence.
ACCESSION   DAOQII010000118 DAOQII010000000
VERSION     DAOQII010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_7299_cov_4.792518"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..625)
                     /locus_tag="PLF48_06885"
     CDS             complement(56..625)
                     /locus_tag="PLF48_06885"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNF49084.1"
                     /translation="MFLEPNVNKSKTGWIEVVCGSMFSGKTEELIRRLKRAKIANLKV
                     EIFKPMIDTRYHETEVVSHDANSIMSTPVSSSQNILLLSNDVDVVGIDEAQFFDEGLS
                     EVCEELASRGIRVIIAGLDMDFLGRPFGPIPALLAKAEYITKVHAICVQCGALANHSY
                     RLVASDSKVLLGEKESYEPRCRACFNKDK"
     gene            679..2040
                     /locus_tag="PLF48_06890"
     CDS             679..2040
                     /locus_tag="PLF48_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49085.1"
                     /translation="MEIRNALQKIENYLTVVSNLNKTILAEGAMSREELLLMKKYLNS
                     CIDRITDIERKLSINMIETPPVETTTVLTNTTEIVEADKNKEIVAVDENQHIEEMEAT
                     LNTEQQQELEMALDEIEQQIEKQEEAKTETLFTVVEEEVVATPELKTEETNITPVAEL
                     VTNETENKLMEESTKEEVIAEVIQAAVENEIKKEEIQEQKEEEQTSVADHIYNSITQT
                     IDELKNKAEEIKNSEFVQNAETKLENFAAVTQQKMEAMVDDFKKEEDKIEKDKESLVA
                     KMEDRKKQETSFFEKLEDKFKEDNSKPLFHLFDDGKEDFHETLLNKKQEDTKLFTLEK
                     NEVKDEPVVETKSETIQNTESLNDIFKTNTVTHVETIQQPKVRKSLQELIMLNDKFVF
                     VRELFGNQFAEYDTAIKSLENMNSLNDAENYCRNQLWTKFNWNDKVAYQMRFFDVLQK
                     RFG"
     gene            complement(2558..3814)
                     /locus_tag="PLF48_06895"
     CDS             complement(2558..3814)
                     /locus_tag="PLF48_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNF49086.1"
                     /translation="MQIPLAERMRPQSIDQIIGQEHLLGEGKPLRNLLDKNKLHSVIF
                     WGPPGVGKTTLARVLATQAGLSFEQLSAISSGVKELRAVIDEAKTFGTKVLFIDEIHR
                     FNKSQQDALLGAVENGTIILIGATTENPSFEVNSALLSRSQVYVLNALDETALKQIAH
                     LALQQDDDLKSLNLQIEEYDALIYFSGGDARKLLNILELVAENAGEEKTITNKMVEQI
                     IQTYTSRYDKTGEQHYDIISAFIKSLRGSDANAALYWMARMINAGEDPKFIARRMLIL
                     ASEDIGNANPNALLLANATFEAVEKIGYPECRIILGQCAVYLATSPKSNASYMAINRA
                     LELAEKTSQLSVPLAIRNAPTKLMKNLNYGKGYQYAHDYENNFADMEFLPDEIKGTKF
                     YEPGKNARENELRQFLKVRWKEKYGY"
     gene            complement(3893..4507)
                     /locus_tag="PLF48_06900"
     CDS             complement(3893..4507)
                     /locus_tag="PLF48_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49087.1"
                     /translation="MKYILVISLLLSFFPAFAQKKTATDKAMASACECINKTDKKLNK
                     EGLKIEVEKCVTQAMLANLQGLVDEYDIDMTDEKSAYEMGKQLGIKLAAKCPAFIELF
                     ANSELLGKETNQTSEAAESEFTEGTLKEIIIEDVIYLVIVEANGDTRNFVLYTYFNGA
                     SEFVQSLDLYKNKEVKIGWTTQKVFDKKSNTFVDKKIVTALQLW"
     gene            complement(4539..5072)
                     /locus_tag="PLF48_06905"
     CDS             complement(4539..5072)
                     /locus_tag="PLF48_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNF49088.1"
                     /translation="MSIPADLSVYLNIALRSAAVYAFMLFAFRVFGKRELSQLSISDL
                     VLVLLISNAVQNAMVGENTSLQGGLVAATILFLLNYIVGYLKFKYRNVNKILHSEPVI
                     LIYDGKINKKNMNQNFLTISELEAAIREHGVEKIEQVHSAILENDGNISVISNSDSNL
                     KKTVIKRKRAQKTLIDM"
     gene            complement(5318..7231)
                     /locus_tag="PLF48_06910"
     CDS             complement(5318..7231)
                     /locus_tag="PLF48_06910"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNF49089.1"
                     /translation="MKTYIPVAVICFHLCCYLNIYSQYFSTAYPNNRSVADTTIAPFY
                     HGVASGDPLSDRVIIWTRVTSNDIFDTVYWYVATDSTMENIISKGKYFTSANKDHTVK
                     IDVTGLTANTYYYYTFYSKGRFSQVGRTKTMPVGEVNNLRIGVLSCSNYSFGYFNAYD
                     ILNERNDVDFIMHLGDYIYEDGYHNDGIDTLHRRVFPEYDAYDKPSYEMRYSWYRLDP
                     SLRELHRQYPWVVIWDDHEFANDAFRDTSFQHHPLTQGTWADRKKAAIEAFKEWIPFR
                     EDTAKCNIINYTQHIGNLADIIYTENRIQRDTFDNSLQFLSLLIGTNNPLSYANPNLS
                     ALGSKQLNWICNELKTSDAKWKILANQLAFTPFFIQGPLRIKTRFPSSWDFYPWDRKH
                     ILDTVAKNNIKNFVVLSADAHVAMAFDVVHDSIPYSASNGTGSLGVEFVCDNAVYGNF
                     FSGMEPFMFQYNPQLKYLNTNSQGFYILDIKPSRMCCDYWQIDSINTLNTKKTYLKTF
                     CTEDNMCHLKEEVGPIPPVNTYAPLLSYHVNTAITITGVPLVYGNIEVLKLYPNPTKN
                     YINIQFSNLETNNVQVFLYDMTGRMLQEVNLGLKIKGLNSCTIHFPNFASGQYILDFV
                     SEGIHNQNKIIIE"
BASE COUNT         2454 a         1340 c         1212 g         2293 t
ORIGIN      
        1 cgggataagc atttcaaaat aaaatagttg atttatttat caagaatcag ctcatttatt
       61 tgtctttatt gaagcatgct ctgcatcttg gttcgtaact ttccttttcg cccaacaaca
      121 ccttgctgtc gctggctact aagcgataag aatggttggc taaagctccg cattgtacac
      181 aaatggcatg tacttttgta atatattctg cctttgccaa gagcgcagga atggggccaa
      241 atggtcttcc taaaaaatcc atatccaagc cggcaataat cactctgatt cctcttgaag
      301 ccaactcttc acacacttcc gataatcctt catcaaaaaa ttgggcttca tctatgccca
      361 ccacatccac atcattactc agtaaaagga tattttgtga actgctcacc ggcgtactca
      421 ttatagaatt ggcatcatgc gaaaccactt cggtttcatg atagcgcgtg tctatcatgg
      481 gtttaaaaat ttctactttg agattggcta tttttgcacg cttaagcctg cgaattagtt
      541 cctccgtttt tcctgaaaac atggatccgc agacgacttc gatccatccc gttttagact
      601 tattaacatt tggttctaaa aacattagac aactttgcgt tttagactat aatttcgtcg
      661 aaattacaaa aataaaacat ggaaattaga aatgcactcc aaaaaataga aaactatctc
      721 accgtagttt ccaatctgaa taaaactatt cttgccgaag gagcaatgag ccgtgaggaa
      781 ttattgctta tgaaaaaata cctgaacagt tgtattgaca ggattacaga tatagagcga
      841 aagctttcta taaatatgat tgaaacacct ccggtagaaa cgacaactgt actgactaac
      901 acgactgaaa ttgtagaagc tgataaaaac aaggaaatag tagccgttga tgagaatcag
      961 catattgaag aaatggaagc gacgctaaat acagaacaac aacaagaact ggaaatggct
     1021 ttggatgaga tagaacaaca aattgaaaaa caagaagaag ccaaaacaga gacgctgttt
     1081 accgttgttg aagaagaagt agtggcaaca cccgaattaa aaacagaaga aaccaatata
     1141 acgcccgtag ctgaattagt tacaaatgaa acagaaaata agctcatgga agaatccact
     1201 aaagaggaag taatagcgga agtaattcaa gctgcagtag aaaacgaaat aaagaaagaa
     1261 gaaatacaag aacagaaaga agaagagcag acctctgtgg ccgatcatat ctataattcc
     1321 attacgcaaa ccattgatga attaaagaac aaagctgagg aaatcaaaaa cagtgagttt
     1381 gtacaaaatg ctgaaaccaa gctagagaat tttgctgccg ttacacagca aaaaatggaa
     1441 gcaatggttg atgatttcaa aaaagaagaa gataaaatag aaaaagacaa agaatcactg
     1501 gtggcaaaaa tggaagaccg gaaaaaacag gaaacttcct tttttgaaaa gctggaggac
     1561 aaatttaaag aagacaattc caaaccttta ttccatttat tcgatgacgg aaaagaagat
     1621 ttccatgaaa cactattgaa taaaaagcaa gaagatacta aactctttac actagaaaag
     1681 aatgaagtta aagatgaacc tgttgttgaa acaaaatctg agaccattca aaatacagag
     1741 agcttaaatg atatttttaa gaccaacaca gttacacatg tagaaaccat tcaacaacca
     1801 aaagttcgaa aatcacttca ggaattgatt atgctgaatg ataaatttgt atttgtacgc
     1861 gagttatttg gcaatcagtt tgcagaatac gacacggcaa ttaaatcatt agaaaacatg
     1921 aacagcttaa atgatgccga aaattattgc cgcaatcaat tatggacgaa attcaactgg
     1981 aacgataaag tagcctacca aatgcgcttc tttgatgtac tgcaaaaaag atttgggtaa
     2041 gttaacccat ctttttactt caaaatttgt tttactagct cacttcgttc gctataattg
     2101 cttttagctt acgaagcaac ctgctcaacc attaatacca tttcctacct ttcaaaaaat
     2161 aaattatttt agtaaggtga atatatagat ttttttcgtt tggttgatgt aatgaagaga
     2221 aaatagattt ttaccgcttg atgatgaatg agaaagggac aatggaaggc ttaattaaaa
     2281 aagcatataa ttgtatttat aataatatat ataaatatat aatttttgtt acacttttat
     2341 tacttctaag ttatattttt taaaactcaa atctaacaat attcaattat gtaaatattc
     2401 attttatatt ccatttgatt tatatattaa gaacgataaa cccatgctta acaaaaatgc
     2461 gtttataact tgaattcagc cagcacaacg agttcagcta atattatgct gttactttct
     2521 taacccgaac tctggttaat gactaaaaaa caaaaattca ataaccatat ttttctttcc
     2581 acctgacttt gaggaactgg cgcaattcat tttctcgtgc atttttaccg ggttcataaa
     2641 atttagttcc ttttatttca tccggcagaa attccatatc ggcaaaatta ttttcataat
     2701 cgtgggcata ttggtagcct ttgccataat taaggttttt catgagctta gtaggtgcat
     2761 tccttatagc caatggtact gacaattgac tggttttttc cgccagctcc aaagctcgat
     2821 taatggccat atagctggca ttgctcttag gtgacgtagc taaataaaca gcacattgcc
     2881 ctaaaataat gcgacattca ggatagccta ttttttctac agcttcaaaa gtagcatttg
     2941 ccagcaatag cgcattcggg ttagcatttc ctatatcttc agaagctaaa atcagcattc
     3001 ttctggcaat aaatttcgga tcttctcctg catttatcat acgcgccatc cagtacaatg
     3061 cggcattggc atcactacca cgcaaagatt ttataaaagc agaaatgata tcatagtgct
     3121 gctcacctgt tttgtcataa cgtgaagtat aggtttgtat aatttgctcc accattttat
     3181 tggtgattgt cttttcctca ccagcatttt cagctactaa ttccagaata ttcaagagct
     3241 tccgggcatc tcctcccgaa aaataaatca gggcatcata ctcttcaatc tgtaaattaa
     3301 gcgatttcaa gtcatcatct tgctgcaaag ctaaatgggc aatttgtttc aaagctgttt
     3361 catctaaagc attcaaaaca tatacttggc ttcgggataa tagtgcggag ttaacttcaa
     3421 aagatggatt ttccgtggtg gcaccgatga gaataatggt gccattttcg acagctccca
     3481 gcagtgcatc ttgctgtgat ttattaaatc ggtgtatttc gtcaataaat aaaacttttg
     3541 taccaaatgt tttggcttca tctatgactg cacgcaattc ttttacgcca gaagatatgg
     3601 cactaagctg ctcaaaggac aaaccggctt gtgtggccag cacacgggct aaagtagttt
     3661 tgcccacacc cggaggtccc caaaaaataa cagaatgaag tttgtttttg tctaataaat
     3721 ttctgagtgg tttgccttcg cctaacagat gctcctgacc aataatttga tctatcgact
     3781 gcggacgcat acgttctgct aatggaattt gcatgaagtg aaaataagaa aagtaaaatt
     3841 acggaatgaa taaattacaa gcccaaaaac tattatagaa aaaatcgctt aattaccata
     3901 attgcaatgc cgttacaatc tttttatcaa caaaagtatt tgacttttta tcgaaaactt
     3961 tttgtgtagt ccatccaatc ttcacttctt tgtttttgta taaatccaac gattgtacaa
     4021 attcagatgc accattgaag taggtataca aaacgaaatt tctggtgtct ccattggctt
     4081 ccacaatgac aagataaatg acgtcttcta tgataatttc tttaagagtt ccttctgtaa
     4141 attcagattc agctgcttcg gatgtttgat tcgtttcctt tcctaaaagt tctgagttgg
     4201 caaataattc tataaatgca ggacattttg ctgccagttt aatgccaagt tgtttaccca
     4261 tttcatatgc acttttttcg tctgtcatat caatatcata ttcgtctacc aacccttgca
     4321 aattagctaa catagcctga gtaacgcatt tttccacttc tatcttcagt ccttctttat
     4381 ttaacttttt atctgtttta ttaatacatt cacaagcaga agccatggct ttatcagtag
     4441 cagtcttttt ctgtgcaaat gcaggaaaaa atgaaagaag caaactaata acgagtatat
     4501 atttcatatt ttctcaaata aatttataat ttaattattt acatatcaat cagagtcttt
     4561 tgtgctcttt ttcgtttaat aactgttttt ttcaggttcg agtctgagtt ggatatcaca
     4621 ctgatatttc catcgttttc cagtattgcg gaatgcactt gttctatttt ttcaacgcca
     4681 tgttcacgaa ttgccgcctc cagctcactt attgtcagga aattttgatt catgtttttc
     4741 ttgtttattt ttccgtcata tatcaaaatc acaggttccg aatgaagtat cttgttgaca
     4801 tttctgtact tgaatttcag ataacctaca atataattca acaaaaacaa gatggttgct
     4861 gcaacaagac ctccttgcag ggaagtattt tcgcccacca ttgcattctg aacagcatta
     4921 ctgataagca gtaccagaac caaatcggat atggaaagct gggacaactc ccttttacca
     4981 aaaacacgga aagcaaacaa cataaaggca tataccgctg cactccgaag agcgatattg
     5041 agataaacgg ataaatctgc tggtatagac attgggataa aagtagtgaa aaaacatgag
     5101 ttaaatataa tatacaaaat aaccatttgc acaactgcat ctaattccac atttatcgac
     5161 ttcagatttt aaatcactaa ttacagctca aaaaatagta tttatggaca tgaagccgac
     5221 atcaagactt tttctaatat tatttctttt cagaaggctt gcaatttata caaggtgaac
     5281 atttattcat acattataca tgaggcattc ggttagttta ctctataata attttatttt
     5341 gattatgtat accctcactt acaaaatcta atatatactg tcccgaagcg aaatttggaa
     5401 aatgaatggt acaactattt aatcctttta tttttaaacc caaatttacc tcttgtaaca
     5461 ttcttcctgt catatcatat aaaaacactt gtacattatt tgtttcgaga tttgagaatt
     5521 gaatatttat ataattttta gttggattcg gatacaattt taaaacttca atatttccgt
     5581 aaacaagagg aacgcctgtt atcgttattg cggtgtttac atgataactg agcagaggag
     5641 catatgtatt tacgggcgga attggaccaa cttcttcttt caggtggcac atattatctt
     5701 ctgtacaaaa tgtttttaaa tacgtttttt tagtattcag cgtattgatg gaatcgattt
     5761 gccagtaatc acaacacata cgacttggtt ttatatcaag aatataaaaa ccttgtgaat
     5821 tggtgtttaa atattttaat tgaggattat attgaaacat aaacggctcc ataccggaaa
     5881 agaaatttcc atatactgca ttatcacata cgaactctac acctaaagac cctgtgccgt
     5941 ttgaggcgga atagggtatc gaatcatgga cgacatcaaa tgccattgca acatgtgcat
     6001 ctgcggaaag tactacaaaa tttttaatgt tatttttagc aacagtatct aaaatatgct
     6061 tcctgtccca tggataaaaa tcccaggaag aagggaatct ggtttttatc cttaaaggac
     6121 cttgaataaa aaatggcgta aatgcaagct ggttggccaa aattttccat tttgcatccg
     6181 atgttttcag ctcattacaa atccaattca attgtttgga accaagtgcc gacaagtttg
     6241 gatttgcgta gctaagcgga ttatttgtgc caatcaacaa acttaagaat tgtagtgagt
     6301 tatcaaatgt atctctctga attctgtttt ccgtataaat aatatctgcc aaattgccaa
     6361 tatgttgcgt ataatttatt atgttgcatt ttgcagtatc ttctctaaac ggaatccatt
     6421 ctttaaaggc ttcaatagct gctttttttc tgtctgccca agttccctga gtaagtggat
     6481 gatgttggaa ggaggtatcc cgaaaggcat catttgcaaa ctcatgatca tcccagatta
     6541 caacccatgg atattgcctg tgcagttctc tgagcgacgg gtctaatctg taccaactat
     6601 atcgcatttc atatgacggt ttatcgtaag catcatattc gggaaataca cgtctgtgca
     6661 aagtatcaat accatcatta tggtaaccat cctcatatat ataatcacct aagtgcataa
     6721 taaaatcaac atcgtttctt tcatttagta tgtcataggc attaaaatag ccaaatgaat
     6781 aatttgaaca agacaaaacc cctatccgca gattattgac ttcgcctacc ggcattgttt
     6841 ttgtcctacc tacttgagaa aaacgaccct ttgaataaaa tgtataatag tagtatgtat
     6901 ttgcagttaa accagtaaca tcaattttta cggtatgatc cttattggca gatgtaaaat
     6961 attttccttt tgaaattata ttttccatag tagaatcggt tgcaacatac caataaaccg
     7021 tatcaaaaat atcattcgat gttacccgtg tccagataat aactctgtcg gagagcggat
     7081 cgccagatgc aacaccatga taaaatggtg caatcgtagt atctgcaacc gatcggttgt
     7141 tgggatatgc agtagaaaaa tactgactgt atatatttaa atagcagcaa agatggaagc
     7201 agataacggc aacaggaata tatgtcttca ttttaattat ttattaatac aaatattgtt
     7261 agtctatcaa ttaaaataca aaacaggagc acgtccatc
//
LOCUS       DAOQII010000119         7143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5812_length_7143_cov_5.345372, whole genome shotgun sequence.
ACCESSION   DAOQII010000119 DAOQII010000000
VERSION     DAOQII010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_7143_cov_5.345372"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLF48_06915"
     CDS             complement(<1..222)
                     /locus_tag="PLF48_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49090.1"
                     /translation="MFSKEDVLFECVPNFSEGRNIHIINCIADEIKNTPDVALLNTDM
                     GYDANRTVFTFAGKPDAIIQAGFKAVKKAY"
     gene            complement(620..1408)
                     /locus_tag="PLF48_06920"
     CDS             complement(620..1408)
                     /locus_tag="PLF48_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49091.1"
                     /translation="MHSYSYRNLTINALLLMVIAAIVEMILHELGHFFCALYFHASAL
                     SLHHNYVNFDENSLSIFQRIWIAAAGPIISLCIGVIFHFIVTQQKSRNYFFLFNCYFA
                     GHGYIGFFGYLMIAPIAAEGDTGFIMHQLKFPVYITIVIALIATLILRKFIQTITKYI
                     MEFATLEIYQDAALRKKYAKSVLLYPIYLGLIIIILMNLPVPIWISLMAPFSIFSFFF
                     AFGEALKKDYTQIRFNTNDVALFKIQPAIIVSFVLVLIINRLLV"
     gene            complement(1569..1847)
                     /locus_tag="PLF48_06925"
     CDS             complement(1569..1847)
                     /locus_tag="PLF48_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HNF49092.1"
                     /translation="MNENEKEALIWKIAKDRVRFKKSLLVYVTMNCFFWLIWLFTKSG
                     HDTFNGIPWPIWSTLGWGIGIIMQYINAYHNKTHEMEKEVEKLRNEMR"
     gene            complement(1858..2148)
                     /locus_tag="PLF48_06930"
     CDS             complement(1858..2148)
                     /locus_tag="PLF48_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF49093.1"
                     /translation="MKSLPQLDPLLHNTLRLAIMSILVSVKEADFSYLQEATSSSNGN
                     LSVQITKLKDAEYINVKKTFVNNYPKTTCSISKKGIKAFEDYVRNLKFYITK"
     gene            complement(2145..2711)
                     /locus_tag="PLF48_06935"
     CDS             complement(2145..2711)
                     /locus_tag="PLF48_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49094.1"
                     /translation="MEPNESLQIIENMIAKTKGNIATKSKYFLLWGWAVFLTAIAQYI
                     LIRMLFPHAQMVWLSMIIAGIIAVFIAVRDKKTEKSISYVGDAVRKLWQSIGIACILI
                     VVLTIKGTIFSIPVFILIYGVGVFTTGRIIQFKPLIIGGLICFVSSLISVFIPQDANQ
                     LLLLAATVMCSYIIPGHLLSAESKKAIR"
     gene            complement(2797..3738)
                     /locus_tag="PLF48_06940"
     CDS             complement(2797..3738)
                     /locus_tag="PLF48_06940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF49095.1"
                     /translation="MFSLFNPLDFNDPVTYAIPGFVLLIVLEFTLYIRKKLKPGIDYY
                     KDATASIGLGLGSVLIDLPMKAIAIGYLLWFYQFRIFQNLGPDTIQEFASLSWNKSHW
                     WVWIMALFFQDFAFYWHHRLSHVIRILWAAHVNHHSSTNINFAVALRQSWLELLYKDA
                     WYIPLVLIGFHPLMILTLHQINLIYQFLPHTETVKKLPRWFEFIFNTPSHHRAHHASN
                     IKYLDKNYGGIFIIWDRLLGTFREEDDEKPVYGITHNIHSYNLLYITFHEVGNIIRDV
                     KSAPNFKDKLHYIFDAPGWSHTGVDQRAKTLQKKMGY"
     gene            complement(3977..4588)
                     /locus_tag="PLF48_06945"
     CDS             complement(3977..4588)
                     /locus_tag="PLF48_06945"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNF49096.1"
                     /translation="MTFELPALPYDYNALEPHIDARTMEIHHNKHHQAYVTNLNNALA
                     GTDGEGKTLLELITNISKYSPAVRNNGGGHFNHSLFWRIMSPDGGGEPTGKIGEAITA
                     TFGSYDKFKEEFSKAATTRFGSGWAWLCVKDDKSLCVCSSPNQDNPLMDISECPGTPI
                     LGLDVWEHAYYLNYQNRRPDYITAFYNVINWDEVNRMYSAIVG"
     gene            4809..5033
                     /locus_tag="PLF48_06950"
     CDS             4809..5033
                     /locus_tag="PLF48_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49097.1"
                     /translation="MKKLAFSALFGSMLLLGMTSCKKDYTCDCKDGNGNKFDSQTYPN
                     TSITDAKRSCNERENFWKNTTVSSANCTIL"
     gene            complement(5273..6517)
                     /gene="clpX"
                     /locus_tag="PLF48_06955"
     CDS             complement(5273..6517)
                     /gene="clpX"
                     /locus_tag="PLF48_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNF49098.1"
                     /translation="MAKKNYTKCSFCGKSKADTLLLIEGVDACICENCAEQAANIVDA
                     ELGGEFTPKQKSGSAAPKMLLKPIDIKAHLDQYVIGQEDAKKVIAVAVYNHYKRLNQK
                     ANKDDVEIEKSNIVMVGQTGTGKTLIAKSVARLLNVPFTIVDATVFTEAGYVGEDVES
                     IISRLLQVCDYNVSKAEQGIVFIDEIDKLSRKGDNPSLTRDVKEGTQQALLKLLEGTE
                     VLVPPQGGRKHPEQSFTKVNTKNILFICGGSFDGIESIISKRLQTASIGFKKQDEKDI
                     IEKENYLEYITQKDLRSYGMIPELIGRLPVLCHLNPLDKDALRDILTKPKNALLKQYQ
                     KLFDIENIKLEFDDDAIEFIIDKAFEYKLGARGLRSICEAIITDAMFELPSKEGIAEF
                     IVSRTYAEEKVNKSKISKLKIA"
     gene            complement(6716..>7143)
                     /locus_tag="PLF48_06960"
     CDS             complement(6716..>7143)
                     /locus_tag="PLF48_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNF49099.1"
                     /translation="DHKVKAIVLRINSPGGSVYAGLGIYDTMQYVNPKVSTCCVGIAA
                     SMAAVLLCAGEKGGRSALKHARVMIHQPMGGAQGQASEIEITYNEIQKLKKELYDIIA
                     QHSGQTYEKVYKDSDRDYWMTSDEAKEYGMIDEVLLRKA"
BASE COUNT         2256 a         1320 c         1237 g         2330 t
ORIGIN      
        1 gtaagctttt tttacagcct tgaatccggc ttgaatgatt gcatccggct tgcctgcaaa
       61 tgtaaataca gtgcggttgg catcatagcc catatcagta tttaataatg caacatccgg
      121 tgtgttttta atttcatcag ctatacagtt tatgatgtgt atatttcttc cttccgaaaa
      181 attcggcaca cattcaaaca atacgtcctc ttttgaaaac atgtacgaat gtaggaaata
      241 ttacatctta atgttacaac ctatggttat ttaaaagtaa accaagttca agtgcatttt
      301 ctgaaacaga tttgcgaaat cttaattatt tgagtaatcc acccccaccc ctgcgtgagg
      361 ggagtttatg gtgtgcatga ttagactata tcttttttag gtaaactgat taagttatgc
      421 cacttagttg cgcatctaat tagttaatta gtttagtagt ggtacaaatt ttattctttt
      481 cagaaagcca cgtagtggcg acaccttttc ttgtctgaat tattttagag atgcgttgtg
      541 cttgcctttg cattaatgta attgttgtta gaatcgataa gtgcatatac caacattaaa
      601 gaacaaagcg agctagtact tataccagta aacggtttat tatcaatact aacacaaaac
      661 tgacaataat agcaggctga attttaaata aagcaacgtc attggtattg aaacgtattt
      721 gcgtataatc ttttttcaga gcttctccaa aagcgaagaa gaatgaaaat atactgaatg
      781 gtgccatcag actaatccag ataggtacgg gtaaattcat cagtatgata attattaaac
      841 caagataaat cggatacagc agtactgatt ttgcatattt ttttctgagt gccgcatctt
      901 gatagatttc caaagtagca aactccatga tatactttgt aattgtttgt ataaattttc
      961 tcagtataag tgtggctatt agagcaatta caattgtgat ataaaccgga aatttgagct
     1021 ggtgcataat aaatccggta tctccttcgg ctgcaattgg tgcaatcatc aaatagccaa
     1081 agaaaccgat atatccgtgt cctgcaaaat aacaattaaa caaaaagaaa taatttctgc
     1141 tcttttgttg agtaactata aaatggaata taactccgat acataaactg attatcggac
     1201 ctgctgcagc aatccagatt ctttgaaata tgctcaagga attttcgtca aaattcacgt
     1261 agttgtgatg gagacttagt gcagacgcgt gaaaatacaa ggcacaaaag aaatgaccca
     1321 gctcatgcaa tatcatttct acgatggctg ctataaccat aagcaatagg gcatttatag
     1381 taagatttct ataactataa ctatgcattt tttatttttt ttggaattta tatatgttca
     1441 cacttttctt aagaatagat agcagctgat ttttcatacc gactatcata tgtaatatat
     1501 ctaatcgatt ttttcttctg ctaatgataa gctcataaat cttacatgat gtttcttgat
     1561 ctgcactatt atcgcatttc gtttctcagt ttttcaactt ctttttccat ttcatgtgtt
     1621 ttattatggt aagcatttat atactgcatg ataatgccga ttccccatcc gagcgttgac
     1681 caaataggcc atgggatgcc attaaatgtg tcatgccctg atttggtaaa tagccaaatt
     1741 aaccagaaaa agcaattcat tgttacgtaa acaagcaagc tttttttaaa acggactctg
     1801 tcttttgcaa tcttccagat taaggcttct ttttcatttt cattcatgat attagattta
     1861 tttagtgata taaaatttaa gatttctgac atagtcttca aatgccttta tccctttttt
     1921 agaaatactg caagtcgttt tgggatagtt attgacaaat gtttttttga cattaatata
     1981 ttctgcatct tttaatttag taatttgtac actcaagttt ccgttactgg atgaagtggc
     2041 ttcttgtaaa tacgaaaaat cagcttcttt aacagataca agaatagaca taatggctaa
     2101 gcgcaatgta ttatgtaata atggatctag ttgcgggagt gatttcatct gattgctttt
     2161 ttgctttctg cagataacag atgtccgggt atgatatatg aacacattac cgttgccgct
     2221 aacaataata attgatttgc gtcctgcgga atgaatacac tgataagcga actgacaaaa
     2281 caaataagac caccaattat taagggttta aactgtatga ttctgcccgt ggtaaaaacg
     2341 ccaacaccat aaattagtat aaataccggt atagaaaata ttgtgccttt aattgtaagt
     2401 actacaataa ggatacaagc aataccaata ctttgccaca atttgcgtac agcatcgccg
     2461 acataagaaa tggatttttc tgttttctta tctcgaacag caataaatac agcaataatt
     2521 cccgcaataa tcatgctcag ccacaccatt tgagcatgtg gaaataacat acggattaaa
     2581 atatattgcg ctattgcagt aagaaatacc gcccaacccc atagtaagaa atacttgctc
     2641 ttggtcgcta tatttccttt tgttttggct atcatatttt ctatgatctg caggctttca
     2701 ttcggttcca tagtttgtaa attaagttca aaaataaagt agtttatttt ataaaccaat
     2761 tttatcacaa gaaagtttcc aaaaaaaata ttttttttaa tagcccattt tcttctgtaa
     2821 agttttggca cgctggtcaa cgccggtgtg cgaccaaccc ggtgcgtcaa aaatataatg
     2881 cagcttatct ttgaaattag gagcagattt tacatctcgt atgatattgc ccacttcgtg
     2941 gaaagtaata tacaacaaat tataggaatg aatattatgc gtgataccat aaacaggctt
     3001 ttcatcatct tcctcgcgaa aggtgcccaa cagcctgtcc catataataa aaatgccgcc
     3061 atagttctta tccaagtact tgatattgct ggcatggtgt gcgcggtgat gagaaggtgt
     3121 attaaagata aattcaaacc aacgaggcaa tttcttcaca gtttctgtat gtggcaaaaa
     3181 ctgataaatc aaattgattt gatgcaatgt caaaatcatt aatggatgaa atccaataag
     3241 taccaaagga atataccatg catctttata taataattct aaccaacttt gacgaagggc
     3301 tacggcaaaa ttgatattgg tggatgaatg atgattgaca tgtgccgccc acaatatccg
     3361 aataacatgg ctgaggcgat ggtgccagta aaaggcaaaa tcctgaaaaa acaatgccat
     3421 tatccaaacc caccaatgcg atttgttcca gcttaaactg gcaaattcct gaatagtatc
     3481 aggtcctaaa ttttgaaaaa tgcggaactg ataaaaccaa agcagatagc caatggcaat
     3541 tgctttcatg ggtaaatcga taagaacgga acctaatcct aagcctatac tggcagtagc
     3601 atccttgtaa taatcaattc cgggctttag ttttttgcgt atatacaagg tgaattccaa
     3661 tacgatgagt aaaacaaatc ccggaatggc atacgtaacc ggatcattga aatcaagggg
     3721 gttaaataga gaaaacatac gatagtaaga tacggatttt ttgttaaaaa ttatgctgaa
     3781 attgctacaa cctacgaggt taaataagat aatactggat gaaagatggc agttttattg
     3841 cattttagtc gtcaacctac gaggtcacaa ttggtattct gttgaatatg attagtgaag
     3901 acttgtattt gtaagctaat attaaaaaag cccgcgaaat tcgcgggctt tttttaaaaa
     3961 ttatttttcc tgattattag ccaactatag cgctatacat cctgttcact tcatcccagt
     4021 tgatgacatt gtagaatgca gtaatataat ccggtctgcg gttttggtaa ttcaagtaat
     4081 aagcatgttc ccatacatcc aagcctaata ttggagttcc cgggcattca gatatatcca
     4141 tcaatggatt atcttgattg ggcgaggagc atacacaaag agacttgtca tctttgacac
     4201 acaaccatgc ccagccagaa ccaaaacgcg ttgtagctgc cttactgaat tcttctttga
     4261 atttgtcata agagccgaat gttgctgtta ttgcctcgcc aatttttccg gtaggctcac
     4321 ctccaccgtc aggtgacatt attctccaaa acaaagagtg gttaaaatgt ccgcccccgt
     4381 tgtttcgaac ggctggcgaa tacttagaga tattggtaat cagctccaat aatgtttttc
     4441 cttcaccatc tgttcctgcc aatgcattat tcagattggt tacataagct tgatggtgtt
     4501 tattgtgatg gatttccatc gtgcgtgcat caatgtgcgg ttctaaagcg ttgtaatcat
     4561 aaggcaacgc cggtaattca aatgtcatat tatttatttt aagagtgaaa aaactaattg
     4621 cttgtaaaat taagaaaaat gaaactttta attgatagaa aaaatctata tagcgatagg
     4681 attgaagcac taagtcagca tgaagtgcat tgtaatcctg aaacatttga tagaagttgt
     4741 ataatgaggc taaagctgat ttgaaccatt tttgcagtat aattattaac aacgaaaaaa
     4801 atgaaaaaat gaaaaaacta gctttctccg cactatttgg ttcaatgctg ttattaggca
     4861 tgacttcttg taaaaaagat tacacttgcg attgcaaaga tggaaatgga aacaagtttg
     4921 attctcaaac ttatcccaac acttctatta cagatgctaa acgatcttgt aatgaacgcg
     4981 agaatttctg gaaaaatacg acagtgtcat ctgccaattg tactatttta taaataatac
     5041 aactattaac ctgagtcagg gttaagaaaa ataagctgaa aaatagttga tttatcgttt
     5101 tttcctccac tttaacaagg tggaggaatt aaaaggaggt ggttctacat aaaaaatcaa
     5161 ctaattttaa attaaaatgc ttatcccgaa ctcaggttat tatgcagatg catgtttatt
     5221 tgattaaaaa aggcatcttt cggggatgcc ttttatcttt tataaaaaga aactatgcta
     5281 tcttgagttt tgaaatcttg gatttattca ccttttcttc agcatatgtt cttgatacaa
     5341 taaattcagc tattccttct ttggacggta attcaaacat ggcatccgta attatggctt
     5401 cgcaaataga acgcaaacca cgagcgccta acttgtattc aaaggctttg tcgatgataa
     5461 attctatggc atcgtcatca aattccagtt tgatattttc tatatcaaac agcttttgat
     5521 attgttttaa taaggcattt ttaggtttag tcaagatatc gcggagcgca tctttatcta
     5581 aaggattcaa atggcacaaa accggtaaac gtcctatcaa ctccggtatc atcccgtagc
     5641 tgcgcaagtc tttttgcgtg atgtattcca ggtaattttc cttttcgatt atatcttttt
     5701 catcttgttt tttaaaacca atagaagccg tttgcaatcg cttggaaata atgctttcaa
     5761 taccatcgaa agaaccaccg caaataaaca ggatattctt agtattcact ttggtgaaac
     5821 tttgctccgg gtgttttctg ccaccttgag gaggcaccaa tacttcagta ccttccaata
     5881 attttaaaag tgcttgctgc gtaccttctt ttacatcacg ggtgagtgaa ggattatcgc
     5941 ctttgcgaga caatttatca atctcatcaa taaacacgat accttgctct gcttttgaaa
     6001 cattgtaatc gcatacttgc aacaatctcg atatgatact ctctacatct tcgcccacgt
     6061 acccggcttc tgtaaatacc gttgcatcta ctatagtaaa aggcacattg agcaagcgtg
     6121 caacagattt ggctatgagt gttttgcccg taccagtctg tcctaccatc acgatgttgg
     6181 atttttcaat ttccacatca tctttgttcg ccttttggtt gagtctttta taatgattat
     6241 aaaccgcaac cgctattact tttttggcat cttcctgtcc aatgacatat tgatccaaat
     6301 gcgctttgat atcaataggt ttcagcagca ttttaggtgc agctgaaccc gatttttgtt
     6361 tgggggtaaa ttccccaccc aattcggcat ccacgatatt tgcggcttgt tctgcacagt
     6421 tttcacaaat acaagcatca acaccttcaa ttaataaaag cgtatctgct ttgcttttac
     6481 cgcaaaaact gcatttcgta taattttttt tagccaaaac tactattaat gtttaatatt
     6541 aaatgagaat aaagatgaaa gggtaattag taattagtat tgagatttta gagtcaagaa
     6601 tcaagaacca agagccaaga gattgtattg attattgtgt aagtgaacta atatccaatg
     6661 tctaataacc aaatcactaa tcactaatca tcaatttcta atgactaatg accaattatg
     6721 cttttctaag taatacttca tctatcatgc cgtattcttt ggcctcgtca gaagtcatcc
     6781 agtagtcgcg gtcagaatct ttatatactt tctcgtacgt ttgtccgcta tgctgagcaa
     6841 tgatatcgta taattctttc tttagctttt gaatctcgtt gtacgtgatt tcaatttcag
     6901 atgcttgtcc ctgcgcaccg cccatcggtt gatgtatcat cacgcgggca tgttttagcg
     6961 cagagcgacc gcctttttct ccggcacaaa gtaatacagc tgccatagac gcagcaatac
     7021 ccacgcaaca agtagacact tttggattta cgtattgcat cgtgtcataa atacctaagc
     7081 ccgcataaac actgccaccc ggtgaattga tacgcaagac aatagctttc accttatgat
     7141 cga
//
LOCUS       DAOQII010000120         7127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5838_length_7127_cov_4.856052, whole genome shotgun sequence.
ACCESSION   DAOQII010000120 DAOQII010000000
VERSION     DAOQII010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_7127_cov_4.856052"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLF48_06965"
     CDS             <1..609
                     /locus_tag="PLF48_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49100.1"
                     /translation="QYGNTINKIRFSYSNSNKLMSINTFYDAKYMADDYVKIGNSNFN
                     YTGNELSSFTKHEINFRTNGMFSDTLNSMDGTYYFSYPSGFSNQSNLIGFDCNDLVLS
                     SFISGLFYFDINSSGGYNFNSLYNVMFSINKLSSFNTKCDKLIEDIQFNRILQNAATS
                     TVNINVQYTFDPAKNNRIKTMKITNDFGGNLNKILYTFYYKE"
     gene            complement(1039..2310)
                     /locus_tag="PLF48_06970"
     CDS             complement(1039..2310)
                     /locus_tag="PLF48_06970"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNF49101.1"
                     /translation="MVDVILGLQWGDEGKGKIVDYLADQYDIIARFQGGPNAGHTLVI
                     NGKKHVLHTIPSGIFREGKINFIGNGVVIDPVTIIKEIEKLEAEGVDVQSRLIISKRA
                     NLILPTHKILDAASEAAKGASKIGSTLKGIGPTYMDKTGRNGIRIGDILEKDFGVKYE
                     LLKAKHLQLLQIYHYDFELNEPEDEWFDAIDKLKKYRQEESVYYINDAIKSGKKILAE
                     GAQGSMLDIDFGTYPYVTSSNTITAAVCTGLGIAPHHIGEVMGITKAYCTRVGSGPFP
                     TELLDETGELLRKEGSEFGATTGRPRRCGWIDLPALLYTCLINGVTQLIITKADILHI
                     FEEFGVCTAYRYDGKESIELPFDINHITIAPVLKSFKSWGKIVNNEKVNEALQAYIDY
                     LEAYLKVRVTMVSNGPAREDLIELDANKLLA"
     gene            complement(2421..5330)
                     /locus_tag="PLF48_06975"
     CDS             complement(2421..5330)
                     /locus_tag="PLF48_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNF49102.1"
                     /translation="MKRVVLFLLWCSMINVFAQSQLPPFLQHPNNTWADSVLNSMSLD
                     EKIGQLFMIAAYSNRDETHYSTIEKYIKDYKIGGLIFFQGTMEKQAELTNRYQAATKL
                     PLWIGFDGEWGLNMRLENTIAYPRQLTLGAVENDNLLYDMGVAFARQMKRIGININFA
                     PDVDVNINPNNPVINDRSFGDNKFNVVRKAYHYMKGMQDNGLMACAKHFPGHGDTDVD
                     SHKDMPVLTQSRERLNAIELYPFNQLIRYGVSSVMVAHMNVLAIDNTPNLPSTLSPKV
                     IQKTLKDEMQFKGLVFTDALNMKGVAKNYPSGVAEVKAILAGCDVLLFPDSVDLAVKG
                     IRKAVADKVISEEQLNERVRKILLAKYWMGLNNYQPINLENLRNEVQTESDFYLKYKL
                     LASAITVPKDSLSQIPIITLQRKIAHVAVGYGKPNRFGEYLDNYTQIDHFYISRDDTA
                     TKFNALLQKLKSYNIVIADVQNMSRYASKNYGTTENELHFLSQLNKQNRTILTVFGSP
                     YSLKYFQKFPTIVLGYTEEPEAQEIAAQIIFGARGAHGILPVSVGALAFGEGICTIDG
                     MRLKYGPTLDLNIPTVAFNQIDKIAQTAIDNGVMPGCQILVAKDGEVIYEKAFGYHTY
                     DNDIKTTTNDLYDLASVTKITSTLPLIMKLHEKGKLNVNYRLADYYTYDSTCNKGNLI
                     IKEILCHQAGLPAWIPFYKNTIDSLGNPMPEWYARQKDDSFSIKITDNLFLKKTFADT
                     IWKKIYSCDLQSKEYRYSDLGYYLMKQIVEKIQHDSLENMARKAFYAPLGMNYTLFNP
                     KWKGYDLSKINPTENEKIFRQTLLLGTVHDQGAALLGGVGGHAGLFSNANDLAKYFQM
                     LLNKGYYGGFQYFDPGTVNYFTSKQYAGNRRGIGFDKPAPKGQDGPTCDSASPESFGH
                     TGFTGTMVWADPKYDLIYIFLSNRVYPTADNKKLINQGTRTKIQQVIYDAIGAGK"
     gene            complement(5914..6420)
                     /locus_tag="PLF48_06980"
     CDS             complement(5914..6420)
                     /locus_tag="PLF48_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49103.1"
                     /translation="MKIETSRIYLVEITSADSDFMFRLLNSPKWLQFIGNRNIQTIED
                     ATHYIEHIIRPSYEKNGFGYYKICLNKSDAAVGICGLIKRDFLDDIDIGYALLPEYEG
                     KGFALEATQALFGYAESKLGLSNILAFTTPDNERSIHLLEKLGFSFVKTFLYPETDEE
                     LNLYASKV"
     gene            complement(6477..>7127)
                     /locus_tag="PLF48_06985"
     CDS             complement(6477..>7127)
                     /locus_tag="PLF48_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF49104.1"
                     /translation="FFTQKILDRKQLKLDKNTLVVGETHIVLKALSYLGITKPPPNSY
                     PICLQQFLKRKIWESTVRELVYNLRHDMFESGVFAKPKSDAKKFTGCVLNSLSDLYKL
                     QNASLNTSIYCSELVEWKSEYRVFVVNSKIVGIKNYAGSNEISLDISLVEEAIQIFGK
                     TDERTIAYGIDFGIMKNNETALIEWNDGYSLGSYGLEKEIYTDLIIKRWEELTMIL"
BASE COUNT         2168 a         1368 c         1269 g         2322 t
ORIGIN      
        1 caatacggga ataccataaa taaaattcgt ttttcctatt caaacagtaa taaattaatg
       61 agcatcaata ccttttatga cgctaaatat atggcagatg attacgttaa gattggtaac
      121 tcaaatttta attatacagg aaatgagctt tcttcattta ccaaacatga gattaacttc
      181 agaactaatg gaatgttttc tgatacgcta aatagtatgg acggtacgta ttatttcagc
      241 tatccttctg gtttttctaa ccagagtaat ttaataggat tcgattgtaa tgatttagtc
      301 ctatcttctt ttatttccgg gctattttat tttgatatca atagttccgg tggttataat
      361 tttaatagct tatacaatgt catgttttct ataaataaat tatcttcatt caatacaaag
      421 tgtgataaat taatagaaga tatacaattc aacagaatac ttcaaaatgc tgctacaagt
      481 actgtaaata taaatgttca atacaccttt gaccctgcaa aaaataaccg gataaaaaca
      541 atgaagataa ctaatgattt tggtggaaat ctaaacaaaa tactatatac gttttactac
      601 aaagaataat taattcctac gaaccgtaaa ctcccttctc ggaggggctg ggggtgggtt
      661 aatactcgcc cttggcaggt gttattacct tccaattcgt ttcctcgcag agtctctgag
      721 ctaaggcaat aagcaaaggc agaactctgc gaaagtaaag gaaatccgtt cttttcggga
      781 tttctcctcg tcatccttcc ttgtcgaaat gacgtattct ctgattgatg ggattcctat
      841 cgcgtctcgc ccgaggcgag agaggaacaa cacagagccc cgtaggaacg caatatcagt
      901 aacatgaaat atagccccgt aggggcgaaa caagcacctt tagttagagt gaatattcaa
      961 ttctgtgcta atagtgcagt atgatcaatc taccaaatga aatcattaat tcattatctg
     1021 attgccaaat taaactaatt atgccaataa tttgttcgcg tcaagttcta ttaagtcttc
     1081 gcgcgccgga ccattggaca ccatcgttac acgcactttc aggtaagctt cgagataatc
     1141 aatataagct tgtaaagcct cattcacttt ttcattattc acaattttgc cccaagattt
     1201 aaaggatttc agtacaggag ctatcgtgat atggttgata tcaaatggga gttcaatgct
     1261 ttcttttcca tcatagcggt aagctgtgca aacgccgaac tcttcaaaaa tatgcagaat
     1321 atctgccttg gtgatgatga gctgcgttac gccgttaatc aaacaggtat aaagcaatgc
     1381 aggcaaatct atccagccgc agcgtcgcgg acgaccggta gtagctccaa attcgctgcc
     1441 ttctttgcgc agtaattctc ccgtttcatc caatagttca gtaggaaaag gtccactgcc
     1501 aactcgggta caataggctt tggtaatacc cattacttcg ccaatatgat gaggtgcaat
     1561 acccaaaccc gtacatacgg ctgccgttat cgtgttggag gatgtaacat aaggataagt
     1621 gccgaaatca atatccagca tggagccttg cgctccttct gccagtattt ttttgcccga
     1681 ttttatcgca tcattgatat agtaaacact ttcttcctgt ctgtattttt tgagtttatc
     1741 aatcgcatca aaccactcgt cttccggctc attcaattca aaatcatagt gatagatttg
     1801 caaaagttgt agatgcttgg ctttgagtaa ttcgtatttt acgccaaaat ctttctctag
     1861 gatatctcct atgcggatgc cgttacgtcc ggttttgtcc atataggttg gtccgattcc
     1921 tttcaaggtc gagccgattt ttgaagcacc cttagctgct tctgaagctg catctaatat
     1981 tttgtgtgtg ggtaatatca gattagcgcg tttggaaata atcagtctgg attgaacgtc
     2041 tacgccttcc gcttctaatt tttctatttc tttgattatc gtaaccggat cgataaccac
     2101 accgttgccg ataaagttga tttttccttc acgaaaaatt ccggaaggaa tggtgtgcaa
     2161 aacatgtttc ttgccattta ttactaatgt atgcccggca ttgggtcccc cctgaaaacg
     2221 ggcaatgatg tcgtattgat ctgctaaata atctactatt tttcctttgc cttcgtcgcc
     2281 ccactgtaat cccaaaataa catctaccat gataatatat aatttaaaaa ttaactaaat
     2341 ttcaatcctc taaattcgga attgtttatg agaatgggaa attaaattat tgaattgctg
     2401 aaagtattcc acaaaacaac ttatttccct gctccaatag catcataaat cacctgctgt
     2461 atcttcgttc gggtgccttg gttgatgagc tttttattat ctgccgtagg atatacacgg
     2521 ttcgacagaa agatataaat caaatcatat ttcggatctg cccaaaccat agtgcctgta
     2581 aatccggtat ggccaaatga ttcgggggag gcactgtcgc aagtcggacc gtcttggcct
     2641 tttggcgcgg gcttgtcgaa tccgattccc cgacgattgc ctgcatattg cttagaagta
     2701 aaataattga ccgtacccgg atcaaaatat tgaaaaccac cgtagtagcc tttgttcagc
     2761 aacatttgaa aatatttggc taaatcgttg gcattggaaa acaaaccggc atgcccacca
     2821 acaccgccta gtaatgctgc accttgatcg tgtacggtgc ccagcagcaa agtttgtctg
     2881 aatatttttt cattttcggt cgggttaatt ttagataaat cataaccctt ccatttcgga
     2941 ttaaataaag tgtaattcat acccagcggt gcgtaaaaag cctttctggc catgttctcc
     3001 aaagagtcgt gttgtatttt ttctacgatt tgtttcatca gataataacc taaatcgctg
     3061 tagcgatact ctttggattg caaatcgcag gaatagattt tcttccagat ggtatctgca
     3121 aatgtttttt tcaaaaagag attatctgtg atttttatac taaatgaatc atctttttga
     3181 cgggcatacc actcgggcat aggattgcct aaactgtcaa tagtattttt ataaaatgga
     3241 atccatgccg gcaaacccgc ctgatggcaa agaatttctt taatgattaa atttcctttg
     3301 ttgcaagtag aatcataggt ataatagtcc gctaaacgat aattgacatt cagtttgcct
     3361 ttctcgtgca acttcataat gagtggtaat gtcgacgtaa ttttagttac agatgccaag
     3421 tcatacaaat cgttggttgt agttttaatg tcattatcat acgtatgata tccaaaagcc
     3481 ttttcataaa taacctcgcc gtccttagct accaaaatct gacaaccggg catcacgccg
     3541 ttatcaatag ctgtttgtgc aatcttgtca atctgattaa acgctacagt aggtatgttc
     3601 aaatccagag taggtccata tttcagacgc atgccgtcaa tagtacaaat gccttcgcca
     3661 aatgctaaag cacctacact aacaggtaaa atgccatgcg cacctcttgc accgaaaata
     3721 atctgtgcgg ctatttcctg agcttcaggt tcttcggtat aacccaacac gatagtaggg
     3781 aatttttgaa aatattttaa tgaatatgga gaaccgaata cagtgagtat ggttcggttc
     3841 tgtttgttta actgtgaaag aaaatgaagc tcgttttctg tagtaccata atttttggaa
     3901 gcataacgac tcatgttttg cacgtcagca atgacaatat tataggattt tagtttttgc
     3961 aagagtgcat taaatttagt ggctgtatca tctctgctaa tgtaaaaatg gtcgatttgc
     4021 gtgtaattat ctaagtattc gccaaaacga ttgggcttgc catatccgac agcaacatgt
     4081 gcaattttgc gttgtaaagt aattattgga atttgactta atgaatcttt aggcacagtt
     4141 atggcactgg caagcaactt gtatttaaga taaaaatcgc tttctgtctg tacttcattt
     4201 cgaagatttt ccaaattgat gggttggtaa ttgtttaatc ccatccagta ttttgctaat
     4261 aaaattttac gaacgcgttc attcagttgc tcttcgctga ttactttatc tgcaacggct
     4321 ttgcgaatgc cttttactgc taaatcaaca ctgtcgggaa acaagagtac atcacatcct
     4381 gccagtatag cttttacttc agcaacaccg gaaggataat ttttagcaac ccctttcata
     4441 ttcagtgcat ctgtaaatac caagccttta aactgcattt catctttcag tgttttctgt
     4501 attacttttg gagaaagcgt agacggcaaa tttggtgtgt tgtcgatggc caatacattc
     4561 atatgggcta ccatgacgct gcttacgcca taccgtatta attggttaaa cggatagagt
     4621 tctatagcat taagtcgttc gcgagattgt gtcaatacgg gcatatcttt atgcgagtca
     4681 acatccgtat cgccatgccc aggaaaatgt ttggcgcatg ccattaagcc gttatcttgc
     4741 atgcctttca tatagtgata ggcttttcgc actacattga atttattatc gccaaaagat
     4801 cggtcgttta tcaccggatt attgggattg atattcacat ctacatcggg tgcgaaattg
     4861 atattgattc ctatgcgttt catttgccgg gcaaatgcga cacccatatc atacaataaa
     4921 ttatcgtttt ctactgcacc gagtgtaagc tgccggggat aagcaatcgt gttttccaaa
     4981 cgcatattca aaccccattc gccatcaaat ccaatccaca acggaagctt agtggctgcc
     5041 tgatagcggt ttgttaattc agcttgtttt tccatcgtgc cctgaaaaaa aatcagccca
     5101 cctatcttgt agtctttaat gtatttttct atggtggaat aatgagtttc atctctgtta
     5161 gagtaagcgg caatcataaa taattgccca atcttttcat ctaacgacat cgaatttaaa
     5221 acagaatctg cccatgtatt atttggatgt tgtaaaaatg gcggcaactg cgactgagcg
     5281 aacacattta tcatagagca ccacaacaaa aacaatacaa ctcttttcat atcactaatt
     5341 aatgcaaaaa aatgtcaaat agtctgccaa acatagcaat ttaagatggt tttaactatt
     5401 cttgattttt aaggaatgtg tatatctatt taattagatg atggtattta ttccagataa
     5461 aactaatttt taccacctag ctgtctgatt atatacaaag caagattatc taccaaatta
     5521 tagcatagct gatttttgag gcgaaatatg gtatgaattt aattgattac ttaaatacaa
     5581 acaagacaaa cataataagc aaacgcttgc gtaaaaagcg agggatggca aaaatcggca
     5641 gctgagtgca ggcttaagcg cagagcgata tttttgcctt gtcttttttg ggtacttttt
     5701 tgactaagaa aaaaagtacc gaaataaaat gaaaagaatc tgcttaaaca aagcagctgt
     5761 taactaagct cgggctaaga aaaataaggt aaaaaatgag gtggttctat ataaaaaatc
     5821 aactattttt ttaattgaaa agcttatccc gaactcaggt taacaaattg ggcaattcca
     5881 tttttattga aaaaaagtta atcaacacag ctactaaact ttacttgcat agagattaag
     5941 ctcttcatcc gtttcgggat agaggaatgt ttttacaaat gagaaaccca gcttttccaa
     6001 gagatggatc gagcgttcat tatcgggagt agtaaaggcg agaatgtttg ataaacccaa
     6061 ttttgattca gcatatccaa ataaagcttg agttgcctct aatgcaaagc cttttccctc
     6121 atattccgga agtaaggcat aaccgatgtc aatatcgtct aaaaaatccc gcttaatcag
     6181 tccgcatatt cccactgctg catctgattt atttaaacag attttgtagt aaccaaaacc
     6241 atttttttca taagagggtc tgataatatg ttcgatataa tgcgttgcat cttcgatggt
     6301 ttgaatgttt ctgtttccga taaattgcaa ccatttgggc gagttgagta aacggaacat
     6361 aaaatcagaa tcagctgaag tgatttcaac taaatatatt ctgcttgttt ctattttcat
     6421 gattaattta aaaaagtaaa ttgaattatt aaggaataac aagtgtttgt tgaattttat
     6481 aatatcattg tcaactcctc ccagcgtttg ataattaaat ccgtataaat ttccttttct
     6541 agtccatatg aacctaacga ataaccatca ttccattcta tcagtgctgt ttcattgttt
     6601 ttcattatac caaagtcaat accatatgca atagtccgtt catcagtttt tccgaagatt
     6661 tgaatggctt cttctactaa agaaatatct aatgaaattt cattagagcc tgcataattt
     6721 ttaattccaa caatttttga atttacaaca aacactctgt attctgattt ccactcaact
     6781 agttcagaac aataaatgct ggtatttagt gaagcgtttt gtaatttata caagtcagat
     6841 aatgaattaa gcacacatcc tgtaaatttc tttgcatctg atttaggttt tgcaaagact
     6901 ccagattcaa acatgtcgtg tcttaaattg taaaccaatt ctctgactgt actttcccag
     6961 atttttcttt ttaaaaattg ttgcaaacaa attggatagg aattgggtgg tggtttagtt
     7021 atacccagat aagaaagtgc ttttaataca atatgtgttt ctccaactac aagtgtattt
     7081 ttatctaatt ttaattgctt cctatctaat atcttctggg taaaaaa
//
LOCUS       DAOQII010000121         7089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5872_length_7089_cov_4.733295, whole genome shotgun sequence.
ACCESSION   DAOQII010000121 DAOQII010000000
VERSION     DAOQII010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5872_length_7089_cov_4.733295"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLF48_06990"
     CDS             complement(<1..883)
                     /locus_tag="PLF48_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNF49105.1"
                     /translation="MDFVKNAIDSNRERYIQEICDWLRIPSVSADPKFKEEVLRTADF
                     LKNEFIKIGMDKVELLPTKGYPVVYAEKITDASKPTVLVYGHYDVQPADPYELWTSPP
                     FEPVIKTTELHPQGAIFARGACDDKGQVYMHVKALEMMLQNDFLPCNIKVIIEGEEEI
                     GSAHLGDFLLENKEKLKADVILISDTGIIDNDTPCITNGLRGLCYMEVEVNGPDRDLH
                     SGVYGGGVDNPLNVLCRLIGSLKDENNHITIDGFYDDVLELSAEEKAQINAVPITVEG
                     FEKSIGLPKSGGEKGYTL"
     gene            1030..3090
                     /locus_tag="PLF48_06995"
     CDS             1030..3090
                     /locus_tag="PLF48_06995"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF49106.1"
                     /translation="MPKIAVVTGASGHIGFHVAKLLLEKNYETHLLIRSVNANVAKLA
                     QAGAQVHQVNLFEPATYSAFLSNADVLFHLAAENTTSVTNAASVLENTDKLTQTVLNT
                     ALDVGIKTIIYTSSVVVLGRSHSPEILLNEKDIVRNPESPYVQGKLLAEQFVATLIKD
                     KNVDIRSVYPSWVVGDNDTGLTPPHKVIKDFLAKGAPVYFDGGISIADVTNVAEGHVN
                     AYEKGSFAQQYVLSGDNITFKRFYEILSDNSNQQMPKIKMPKWVIFIASWIFTKLFKM
                     FGKEFPISPQYVRSVVGSYSWYDCSKAKKDIDYQTKPVEDILKNAIQNDIKRELGLLQ
                     LGKRNTLTNTATSSNEADHETLLITGVPGWLGNRMVDILINGDRFGNWQSSRKVRLMV
                     EPRFKGLLNLPDNFEVVYADITNKNQVTEAVKNVVTVFHLAGAIYPKHVDVLYKVNEV
                     GTKNLVDACIENKVRRMIFMSTDSTCGYGTKEKPVFDENTLPNPYKNYGNSKWLAEEY
                     LLQKTREGKIDGTSLRGFWFFGSFAPERQMNFVNMFYWPRQIVFGNGKNLRSISHVDN
                     IIEAFFQAEKNPKTYGQWYWIGNEQPHPTVDDIYKNIASALGVPYKPLYVPKMVCRLM
                     EKTDTFMGKFNRINSTIQAAGKFDYDIAGNIDKAQRDFGYQPKIDLKMASEELAVLTK
                     KNKK"
     gene            complement(3097..4539)
                     /locus_tag="PLF48_07000"
     CDS             complement(3097..4539)
                     /locus_tag="PLF48_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49107.1"
                     /translation="MRNLLLLRISKFNKDQFSRFCDYTCSPYFNKSERITEIIEILRD
                     NYPDISEEVLKKIAKRFPSASAVNVQLSRMNDLLDDFELQQLIESKQYILQALKYELE
                     LDKKNNVKYYIALQTSERRDFRSSDDLWAEYYKEFSSIALCENNWLNEKQKIIEHSEN
                     SMRAIMDYFLSKYLMIYANKSTHTLGVTNLKDSASSRLVLDYVKSNLDQQSLSVKCLF
                     YIIQLFQSIYKNDELFDKNYESLKNDTFNLLNENISYEINYALIQAVSACVYKGVMQT
                     DYLAKAFEMVDFLISRKLFFTFFHEQQTQQRFILLIKIALGCGQTDWALNFINTQIWR
                     VDKKSQASISNYAMGLVYFNLKEYEKATKLMLKVNLSGSSQFTIDNKLTLLKMYYEVN
                     ELHSFQYQTNSLMKYIKQAKVSKNYQLNVMHSIAIITLFFKCRTSNDIKKMHTEIDKK
                     KLNYLIQNYRDKWYVEKYDLLLNANPTINQ"
     gene            4673..5152
                     /locus_tag="PLF48_07005"
     CDS             4673..5152
                     /locus_tag="PLF48_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49108.1"
                     /translation="MYRITTLLISIFMIGMIFAQQMSPEIICSASGRIQNSTIKIDWV
                     IGESVTATMQNTNNQLSSGFGQPQYLITAINPGNSSFSFFIYPNPTIDKINICSSQNS
                     FDGKYEIWDNIGRLQLNGICNAELAAIDCSKLEKGVYFLILKSNNSDFERCFKIVKQ"
     gene            5162..6079
                     /locus_tag="PLF48_07010"
     CDS             5162..6079
                     /locus_tag="PLF48_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49109.1"
                     /translation="MESIYTIFRHIFNTKKGVVFFSIILLSVGLIQAVVPEAIKYQAV
                     VRDNSGNVISNRNVGVRLSILKDNITGTAVYIETHASTTNNLGLINLEIGKGTAVSGS
                     FSSIDWGGDDYFIKIEIDENGGSNYALVGTSQLVSVPYSLFAKKAGNGTQWSDTVNNS
                     IFYKTGNVGVGTDNPQTPLEVRNSQKGKSKILITGSLPELRFGDTTYSNNGVTIGVAA
                     DSNDLISRSGQGDLVFTNEAYGEGGGYIFGTGVPSYPCVKITDDCKVGIGTDVPVSKL
                     QVKGGDINIEDIGSGVVMKSPEATVGECQ"
     gene            6070..6819
                     /locus_tag="PLF48_07015"
     CDS             6070..6819
                     /locus_tag="PLF48_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNF49110.1"
                     /translation="MSVSNSGQPVFTSINCLTTLSGYNQSNSWGAVASYPFNGNANDT
                     SGNGFNGTIHNATLATDRNGNANACYFFTGYNSGNPDYIELPNYSNFETSGEISISLW
                     CKMDNVGQSPFLFTLTPDNPYDRVCGTYFWNSSTNYSPIWDHGNIYSTGRLYSSSMIT
                     PPTVGTWCHLVFVRSKSSNFMNIYYNGIQIASTSNTTDIVNKNLPLRIGGGGYQSGQY
                     NGYFIGYIDDFKIFNRALNSIEINQIYNSER"
     gene            6883..>7089
                     /locus_tag="PLF48_07020"
     CDS             6883..>7089
                     /locus_tag="PLF48_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49111.1"
                     /translation="MLSLLTGLNSCNPNCPNPTTVHDTLYVHDTTHLHDTANCINCYN
                     AVGCYPFSGNANDVSGNNLNGTVIG"
BASE COUNT         2355 a         1177 c         1271 g         2286 t
ORIGIN      
        1 ataaagtgta ccctttttct ccgcctgatt tcggcaagcc aatgctcttt tcaaagcctt
       61 ctacagtaat cggcacagca ttgatttgtg ccttttcttc agcggataat tcaagtacat
      121 catcgtaaaa accatctatg gtgatgtggt tattttcatc tttaagactt ccaattaaac
      181 ggcataaaac attcaaagga ttgtctacac ctccgccata aacacccgaa tgcaaatctc
      241 tgtccggacc gtttacttct acttccatat agcacaaacc acgcaagcca ttggtgatgc
      301 aaggcgtatc attgtcgata attccggtgt cagaaatcag aataacgtcc gctttcagtt
      361 tttctttatt ttccaaaagg aaatcgccta aatgtgctga gccaatttct tcctcacctt
      421 cgataatcac tttaatattg cagggtagaa aatcgttttg caacatcatt tccaatgctt
      481 ttacatgcat ataaacttgc cccttatcgt cgcaggcacc acgagcaaaa atagcgccct
      541 gcggatgcaa ttccgtggtt ttaataacag gctcaaaagg tggagaagtc cataattcat
      601 aagggtctgc gggttgtaca tcgtaatgcc cataaactaa aaccgtaggc ttggatgcat
      661 ctgttatttt ttcggcatac acgacaggat aacccttagt aggcaataat tccaccttat
      721 ccatcccgat ttttataaat tcatttttca aaaaatcggc tgtacgcaat acttcttctt
      781 taaattttgg gtcggcagaa actgacggaa tgcgtagcca gtcacatatt tcctgaatat
      841 agcgttctcg gttgctgtca atcgcatttt ttacaaaatc catttttttc ttaatttgag
      901 tgtgaaatta atcaaaagaa aagaaataaa aatcagatac aactcatatc cccctctttc
      961 ttaatgctat ttaataattt attctatttt cctgctaaat taatctgcta ttaaacaata
     1021 tatttgtgca tgccaaaaat tgcagtggtt accggagcga gcggacacat aggttttcat
     1081 gtagctaaat tattacttga gaaaaactat gaaacccatt tactcattcg ttctgtaaat
     1141 gccaatgtgg caaaattggc acaagccggg gcgcaggtac atcaggtcaa tttatttgaa
     1201 ccggctacgt attccgcttt tttatcgaat gctgatgtat tatttcatct tgcggcagaa
     1261 aatacgacat cggtaaccaa tgctgccagt gtgctggaaa acacagacaa gctgacgcaa
     1321 accgtattaa atactgccct tgatgtcggt ataaaaacaa ttatctatac cagctctgta
     1381 gtagttttgg gcagaagcca ctctcctgag attttactga atgaaaaaga tattgtacgg
     1441 aatccggaaa gtccttatgt gcaaggcaaa ttgttggcag aacaatttgt tgcaacacta
     1501 atcaaagata aaaatgtgga tattcgcagc gtttatccct cgtgggtagt aggagataat
     1561 gatacaggtt taacacctcc gcataaagtt atcaaagatt ttttagccaa aggtgctccg
     1621 gtatattttg acggcggaat atctattgct gatgttacga atgtcgcaga agggcatgta
     1681 aatgcttatg aaaaaggcag ttttgctcag caatatgtac taagtggtga taatattacg
     1741 tttaaacgtt tttatgaaat actgtctgat aattcaaatc aacagatgcc caaaataaaa
     1801 atgcccaaat gggtaatttt tattgcttcc tggatattta ccaaactttt taaaatgttt
     1861 ggaaaagaat ttccgatatc accgcaatat gtgcgcagcg tggtcggcag ttattcctgg
     1921 tatgattgtt ctaaagcaaa aaaagatatt gattatcaaa ccaaacctgt tgaagatatt
     1981 ttgaaaaatg ccattcaaaa tgacataaag cgggaacttg gtttattaca attaggcaaa
     2041 agaaatacgc tgacaaatac tgcaacttca agcaatgagg ctgaccatga aactttgcta
     2101 ataactggtg tacccggctg gctgggtaac agaatggtgg atattttgat taatggcgat
     2161 cgtttcggaa actggcaaag tagtcggaaa gtgcgcttga tggtagaacc tcgttttaaa
     2221 ggcttactga atttgccgga taattttgaa gttgtttatg ctgatatcac gaataaaaac
     2281 caagtgactg aagcagtaaa aaatgtagtt actgtatttc atttagcagg tgccatatat
     2341 ccgaaacatg tggatgtttt gtacaaagtg aatgaagtgg gtactaaaaa cttagtggat
     2401 gcctgcattg aaaataaggt acgccgaatg atctttatga gcaccgattc gacatgcgga
     2461 tatggaacaa aagagaaacc tgtatttgac gaaaacacgc tacccaaccc gtataaaaat
     2521 tacggcaaca gcaaatggct cgctgaagaa tatctactac agaagaccag agaaggtaaa
     2581 atagacggaa catcattacg cgggttttgg tttttcgggt cttttgcacc ggagcggcaa
     2641 atgaatttcg tgaacatgtt ttattggccg agacagattg tatttggaaa cggcaaaaat
     2701 ttgcgaagta tttcccacgt ggacaatatt attgaagctt tttttcaggc agaaaaaaat
     2761 ccgaaaactt acgggcaatg gtactggatt ggaaatgaac aaccgcatcc gacagtagat
     2821 gatatttata aaaatattgc gtctgcctta ggtgttccat ataaacccct ttatgttcct
     2881 aaaatggtat gccgtctgat ggaaaaaaca gatacgttta tgggtaaatt taaccgcatc
     2941 aactcgacta tacaagcagc aggaaaattt gattacgata ttgccggaaa tatagacaaa
     3001 gcacaacgtg attttggcta tcaacccaaa atcgacttaa aaatggcaag tgaagagctt
     3061 gcagtattga cgaaaaaaaa taaaaaataa tttcgattat tggtttattg taggatttgc
     3121 attcaacaat aaatcatatt tttctacata ccacttatct ctgtaatttt gaattaaata
     3181 gttcagcttt ttcttatcaa tttctgtatg catttttttg atatcatttg aagtacggca
     3241 tttgaaaaac agtgttatta ttgcaataga atgcatgaca ttgagttgat aatttttact
     3301 gactttcgct tgtttaatgt atttcataag tgaattagtt tgatactgga aactatgtag
     3361 ttcattaact tcataataca ttttcaatag tgtaagctta ttatcaattg taaattggct
     3421 actaccgctt agatttactt tcagcatgag tttagttgct ttttcatatt ctttcagatt
     3481 aaaatacact aagcccattg cataattgga tatactggct tgagattttt tatcaactcg
     3541 ccagatttgt gtattaataa aattgagtgc ccaatctgtt tgtccgcaac ctaatgcaat
     3601 ttttattaac agaataaatc gttgttgtgt ttgttgttcg tgaaagaatg tgaaaaataa
     3661 tttccttgaa atcaaaaaat ctaccatttc aaatgctttt gctaaataat cagtttgcat
     3721 taccccttta tatacacagg cagaaacagc ttgtataaga gcataattaa tttcatatga
     3781 aatattttca ttgagtaaat taaaagtgtc attttttaac gattcgtaat ttttatcaaa
     3841 tagttcatcg ttcttataaa tggattgaaa taactgaatg atataaaata agcatttaac
     3901 cgaaagcgac tgttgatcca aattggattt cacataatct aaaaccaatc tcgaagatgc
     3961 tgaatccttt aaatttgtaa ctcctaatgt atgggttgac ttgttggcat aaatcattaa
     4021 gtatttactt agaaaataat ccatgattgc cctcattgaa ttttcactgt gctcaataat
     4081 tttttgtttt tcattgagcc agttattctc gcacaaggct atagatgaaa attctttata
     4141 gtactctgcc cataaatcat cactacttct aaaatctcta cgttcagatg tttgtaatgc
     4201 gatataatat tttacattat tttttttatc caattcaagt tcgtacttca acgcctgaag
     4261 aatatattgt ttggattcaa ttaattgttg caattcaaaa tcatctaaca aatcattcat
     4321 tctgctcaat tgcacattta cagctgaagc actgggaaat cttttggcaa tctttttcag
     4381 tacttcttca gaaatatcag gataattatc acgcaaaatt tctataattt ctgtaatacg
     4441 ttcactttta ttgaaatatg gagaacatgt gtaatcacaa aacctggaaa attggtcttt
     4501 gttaaattta gaaatccgta atagaagtaa gtttctcatt gtattctatt cataatacat
     4561 taaataccaa acacttatta ataaataaaa atattttaaa gtaaaaatag gcaaaataat
     4621 tcttattttc tttattttat tctatttaaa tttgtttaca tattttagaa gagtgtatcg
     4681 cataacaaca ttacttattt ctattttcat gattgggatg atatttgctc agcaaatgag
     4741 tcctgaaatt atttgttctg caagcggtcg tattcaaaat agtacaataa aaattgattg
     4801 ggtaattggt gaaagcgtta ccgccactat gcaaaatacg aacaatcaac tatcaagtgg
     4861 ttttggtcag cctcaatatt taattactgc aatcaatcct ggaaacagca gcttttcgtt
     4921 ttttatatat cctaatccca caattgataa aatcaatatt tgctcaagtc agaatagttt
     4981 tgatggtaaa tatgaaatat gggacaatat cggtcgttta caattaaatg gaatttgcaa
     5041 tgcagaactt gcagctattg attgcagtaa actagagaaa ggagtgtatt ttttaatatt
     5101 aaaatctaac aattccgatt ttgaacggtg ctttaaaatt gtaaaacagt aaataaattt
     5161 aatggaaagt atttacacta tatttcgtca tatattcaat actaagaaag gggttgtttt
     5221 ttttagtatt attttgcttt cagtagggct aatacaagca gttgttcccg aagctataaa
     5281 atatcaggca gtagttagag ataatagtgg caatgtaata tcaaacagaa atgtgggagt
     5341 tcgtttaagt attttaaaag ataatattac cggcacagct gtatatatcg aaacacatgc
     5401 ttctaccacc aacaatttag gactgataaa tttggaaatt ggcaaaggta ctgcagtttc
     5461 aggctctttc agctcaatcg actggggagg agatgattac tttataaaaa ttgaaattga
     5521 tgaaaacggt ggaagcaatt atgctctggt aggtacaagt caattagttt ctgtgccata
     5581 ttccttgttt gcaaaaaagg caggaaacgg aacgcaatgg agtgatactg tcaataacag
     5641 catattttat aaaacaggga atgtaggtgt tggtacagat aatccgcaaa caccattaga
     5701 agtaagaaat agccaaaaag ggaaaagtaa aatattaatt accggaagtt tgcccgaatt
     5761 gcgttttgga gatactacct attctaacaa tggtgtaacg attggtgttg cagctgatag
     5821 caatgatctg atttcacgct ccggtcaagg cgatttagtc tttacaaatg aagcctacgg
     5881 agaaggtggc ggatatattt tcggaacagg cgtaccatcc tatccttgtg taaaaattac
     5941 agatgattgc aaagtaggta taggaacaga tgtccctgtt tctaaattac aagtgaaagg
     6001 cggtgatatt aatattgaag acataggaag tggcgtggtg atgaaatctc ccgaggcaac
     6061 tgttggagaa tgtcagtgag caatagcgga caacctgttt ttacttctat aaattgttta
     6121 actacgctct caggttacaa tcaatccaac tcttggggtg cagtagccag ctatccgttt
     6181 aatggaaatg ctaatgacac aagtggcaat ggcttcaatg gaaccattca taatgcaaca
     6241 ttagctacag accgaaacgg gaatgcaaat gcttgttatt tttttacagg atataattca
     6301 ggaaatcccg actatattga attgccgaat tatagcaatt ttgaaacatc aggagaaatt
     6361 tcaattagtc tttggtgtaa aatggacaat gttggacaat ctcctttttt atttacactc
     6421 acaccagata atccatatga tagagtatgc ggaacttatt tctggaattc atctactaat
     6481 tatagtccaa tttgggatca tggaaatatt tattctacag gtcgactata ttcttcatca
     6541 atgataaccc caccaacagt tggaacttgg tgtcatttag tttttgtcag aagtaaaagt
     6601 tctaatttca tgaatatata ttacaacggg attcagattg catctacaag caatacaacg
     6661 gatatagtaa acaaaaatct tcctctaaga attggaggag gcggatacca aagtggtcag
     6721 tacaacggct attttattgg atatatcgat gattttaaga tttttaacag agctttgaat
     6781 tcaatagaaa ttaatcagat ttacaattca gaaagataat tatttaactc aaaaaaaatt
     6841 atgaagaaaa gaattttaat ttttactggg atagctttag taatgttatc tctattaact
     6901 ggacttaatt cgtgtaatcc gaattgtccg aatccgacaa cagtacatga tacactatat
     6961 gtacatgata caacacatct gcatgacaca gcaaattgta taaattgtta taatgctgta
     7021 ggatgttatc cattttctgg aaatgcaaat gatgtgagcg gaaataatct gaatggaaca
     7081 gtaattggt
//
LOCUS       DAOQII010000122         7066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_5893_length_7066_cov_4.948937, whole genome shotgun sequence.
ACCESSION   DAOQII010000122 DAOQII010000000
VERSION     DAOQII010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5893_length_7066_cov_4.948937"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..992)
                     /locus_tag="PLF48_07025"
     CDS             complement(423..992)
                     /locus_tag="PLF48_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49112.1"
                     /translation="MTCNSYKMRLYRYIYILFLLTLHTGCKNHEFSDNITNRSPYFSA
                     IVGDNDNNIFRNVNFGLKQDVVKKAETSKLFDFAPDHMFYEVTFPDDSSQFQEYADIE
                     YYFNENDILDIMVARIYVNDSVQQSQLYNNLQQYYHAKLGSSAIDKFNGEFWKGQFTQ
                     GKSKPMDYYVFMEPLPKEFGISLEFKLQK"
     gene            complement(982..1320)
                     /locus_tag="PLF48_07030"
     CDS             complement(982..1320)
                     /locus_tag="PLF48_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNF49113.1"
                     /translation="MPYKEKEIQKMYYSISEVAKQFDVAPSLLRFWETEFDILRPKKT
                     SKGNRLYTQKDIDSLRMIYHLVKEKGFTLDGARKKLKEKNTNDTQVHAEVISRLKNIR
                     SFIEKMYNDL"
     gene            complement(1386..2357)
                     /locus_tag="PLF48_07035"
     CDS             complement(1386..2357)
                     /locus_tag="PLF48_07035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF49114.1"
                     /translation="MKKEKYYYNPNTLKYERYKLSWKQRLLRITGFFTSAFFFGFLAM
                     VLGYSLFDKPDEKEAKLEVVKMQERYDILDKRVDALTKVLQDMGDRDDNIYRVIFESD
                     PIPDNMRNGQINQSKVYSELKNVPDGKLIDRVLNKIEKLEKSAYVQSKSFDELSKLIK
                     NKQEMLASIPAIQPVSNKDLKRIASGFGYRIDPVYKTTRFHAGLDFTAETGTPVHATG
                     DGVVEIAGPMGDGYGNKVVINHGYGYHTLYGHNSKILVSVGQKVKRGETIALVGSTGK
                     STGPHCHYEVWKNANKIDPVNFFFNDISPEQYDQLLKLAEQSNQSFD"
     gene            complement(2431..4224)
                     /locus_tag="PLF48_07040"
     CDS             complement(2431..4224)
                     /locus_tag="PLF48_07040"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNF49115.1"
                     /translation="MNLTGNLPAYNAPDTRYFKSKEEFNEAVGKDFIAYANKVLEKGE
                     KLLVGLSHGQSPAGVYQYIFDNYTKIKNPHKIYYSFVNSPLKSQERLVGIFHAGPFLK
                     TLVRTGMITREQVLGRNIDDEHLEAYMEEFNSTLTNFLRENNKSGFDYAFLASNPKGR
                     IAGIERNSKAFESRDVSTLVLLNKEKEITTTPYFLQQTKRIAFLATKSDKRRPLAWLY
                     STTAKPTESPSFIRFIDNVKERLTVFIDDEALTWPQIEIKRDTRFGTTTIRLDLTYPY
                     KENAKEKLPVILLIHGFLGLNSFDGLLTTIPTTKYIAAAMHYGTIPNDLPVDDYSKHV
                     MRNINAVVEFFGDRGHPVYIFDHSMGNIYFLMSDHHIDELPGIKKYLRGRIGANPFFG
                     EEAKHALLGFLDNVIIPSMSFSESIIEKPFVLAFRRIIPLDSKNGVRKRGISFSEMLI
                     DKKDSANSNIWKAMKSRIIYLMANMDSLPHLNRIPIEKALNKIPPKVFAIQVHSALQE
                     SMSFDKQKGLVNMTKLNKQILILKSEKDGVAKFVPRLYEGDDKIQIIDVTNKNETDLF
                     REHLYHMVDPQHTTRIIDNFITEVEATYKSN"
     gene            4404..4814
                     /locus_tag="PLF48_07045"
     CDS             4404..4814
                     /locus_tag="PLF48_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNF49116.1"
                     /translation="MIKQYSLQLPPFEKGLHLITKQIVLQLQNLPEKGLLHLFILHTS
                     AGLMVNENADPDVRIDMENWFNRTVKENEPYYRHTLEGSDDMPAHIKSALTGNSLTIP
                     IVNHKLHLGTWQGIYLCEFRNHGGSRNIMATVIG"
     gene            complement(4836..>7066)
                     /locus_tag="PLF48_07050"
     CDS             complement(4836..>7066)
                     /locus_tag="PLF48_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNF49117.1"
                     /translation="LAKVFENGDFRSGYYRDQTFALATGIVSVDQLFGQLYANPSLED
                     DPHSGGRQMNNHFATRNLFPDGSWKDLIHQKNSAADNSPTAAQMLRALGLAMASKKYR
                     ELQDQIGENGFSKNGNEICFATIGDASTSEGIFWEVINAAGVLQVPLIVSVWDDGYGI
                     SVPKSLQTTKGSISQVLKGFQRDNKNKGYDIYVVKAWNYPQLISTYKAAADKTRRTHI
                     PALIHVEEVTQPQGHSTSGSHERYKSAERLQWEKDFDCILKFRHWIINSKIATDDELN
                     TLEKEAKNFVAGKKKEAWSKFHGPILEELKEVIAMTNAIALTSVHKELLQEYVIGLQQ
                     SIDPLRRDIMKVVNDVLITVKDENNVHITALKKWRETQLLINEERYSSYQHTGSAYSP
                     LKLPLVKAEYEQKPSIVSGYQILNACFNANFERDKSIVAFGEDVGKIGDVNQAFAGMQ
                     EKFGVERIYDTGIREASIMGEGIGLAMRGLRPIAEIQYLDYLLYGLQPLSDDLACLSY
                     RTKGGQKAPLIVRTRGHRLEGIWHTGSPIQMILGALRGMHLCVPRNMTQAAGFYNLLL
                     RGDEPALVIECLNGYRLKEKMPKNPGDFTVPLGVPEILKEGNDVTLVTYGSCVRIAQE
                     ALENLERVGISIELIDVQTLMPFDIYGVIGESIKKTNRVVFFDEDFPGGATAYMLQQV
                     MEKQHIFRYLDNPPVTLTAKEHRGAFGSDGDYFCKPNAEDVFQTIYNLMRETNPKRFS
                     SI"
BASE COUNT         2094 a         1335 c         1227 g         2410 t
ORIGIN      
        1 taattaaacc tttttttccg acaatctatt ctttatatag taataaaata atttaccaga
       61 attcagcaat tattttttta tggtgcaatt tattgataaa atttactaaa taagcttaaa
      121 attctgacaa atattcttag tggattccca tttagctctt tttatattac agagcagaat
      181 ttttagtata aaataattaa taaatatcaa atttctacaa caccgataca taaattatca
      241 caagctttct tggtattatg attaagtcca caggattttt gttagaaagt taaacatttg
      301 atgaaaactt aaaatatatt aagtattgat aaatacaggc attcttattt tctttaggtt
      361 agtatcgaaa aaaatatgta attagattgg ttatttgtat tataaaataa taaatattaa
      421 ttttattttt gtaatttaaa ttctaaagaa ataccaaatt cttttggcag tggctccatg
      481 aagacatagt aatccatagg ctttgatttt ccttgtgtaa attgtccttt ccagaattcg
      541 ccattaaatt tatcgatagc agaactaccg agctttgcat gataatactg ttgcaaatta
      601 ttatagagct ggctttgttg cacggaatcg ttcacataaa ttctggctac cataatatcc
      661 aaaatatcat tttcattaaa ataatattct atgtcagcat attcctgaaa ttgagagctg
      721 tcatcaggaa atgtaacttc ataaaacata tggtcggggg caaaatcaaa aagcttggat
      781 gtttcagctt tcttgacgac atcctgcttg agcccaaaat ttacattgcg aaaaatatta
      841 ttgtcgttat cacctactat tgcagaaaaa tagggggaac gattggtaat attgtcagaa
      901 aattcatggt ttttacaacc agtgtgaagt gttaataaaa ataatatgta tatatatcgg
      961 tataggcgca tcttgtatga attacaggtc attatacatt ttttcaatga aagaacggat
     1021 gttttttaat cttgaaatta cttctgcatg tacttgtgtg tcgttcgtgt ttttttcttt
     1081 tagttttttg cgtgcaccat ctaaggtaaa ccctttttct tttactaaat gataaatcat
     1141 gcggaggctg tcaatatctt tttgcgtata aagcctgttg ccttttgatg ttttttttgg
     1201 gcgtagtata tcaaattctg tttcccaaaa acgcaacaag gaaggtgcta catcaaactg
     1261 ctttgctact tcgctgatgc tgtaatacat tttttgtatc tctttttctt tatacggcat
     1321 atcgtattat ttaaagatgt ttatcgaagc ttatatcgct attttatttg ttactgtcat
     1381 acgtatcaat caaaggactg attactttgc tcggctaatt taagcaattg gtcatattgt
     1441 tcgggtgaaa tatcgttaaa aaagaaatta acgggatcta ttttattggc atttttccaa
     1501 acttcgtaat ggcagtgagg tcccgtgctc tttccggtgc tgccaaccag ggcaattgtt
     1561 tcccctcgtt tcactttttg cccaacactt actaatatct tactattgtg cccatacaat
     1621 gtgtgataac catagccatg attaatgaca actttatttc catatccatc gcccatcggg
     1681 cctgcaattt ctacaacacc atctccggta gcatgcacag gagtacctgt ttcagcagta
     1741 aaatctaatc ctgcatgaaa tcgggtagtt ttatataccg gatcaatacg atagccaaaa
     1801 cctgatgcaa tacgcttcag gtctttgttg cttacgggct gtatggcagg tatacttgcc
     1861 aacatttctt gtttattttt aatcaattta gacaattcat caaaggattt ggattgcaca
     1921 taagcagatt tttcaagttt ctcaattttg tttagcacac gatcaatgag ttttccgtca
     1981 ggtacattct ttaactcact gtaaaccttt gattgattaa tttgaccgtt tctcatattg
     2041 tccggtatag gatctgattc aaagatgacc cgatatatat tatcgtcccg gtcgcccata
     2101 tcttgcaata ctttggttaa tgcatccact cgtttatcca aaatatcata gcgttcctgc
     2161 atttttacca cttccagttt tgcttccttt tcatcgggtt tatcaaaaag agaatagcca
     2221 agtaccatag ccaaaaaacc gaaaaaaaag gcagaggtga aaaatcctgt aatgcgcaac
     2281 aagcgttgtt tccaactcaa tttgtagcgc tcatatttaa gcgtgtttgg gttataatag
     2341 tatttctctt ttttcatgcc tagcttagcc gcccataaaa atacgactat ctccttattt
     2401 ggcgatatta aatatcaata gcataaaatc ttaatttgat ttgtatgtgg cttccacttc
     2461 agtaatgaaa ttgtcaatga tgcgggtagt atgttgtgga tcaaccatgt gatatagatg
     2521 ttctctgaat aaatctgttt catttttgtt agtaacgtct ataatctgaa ttttatcatc
     2581 tccttcatac agtctcggaa caaactttgc cacgccgtct ttttcacttt taagaattaa
     2641 aatttgttta ttgagtttgg tcatattgac taatcctttt tgcttgtcaa agctcatgga
     2701 ttcttgcaat gcagagtgta cctgaatagc aaatacttta ggtggaattt tattcagtgc
     2761 tttttcaatt ggaatgcgat tgagatgagg caatgagtcc atattggcca tcagataaat
     2821 aatacgcgat ttcatcgctt tccagatatt gctgtttgca gaatcttttt tatcaataag
     2881 catttcagaa aaactgatac ccctttttct tacgccattt ttagagtcaa gaggaatgat
     2941 gcgacgaaat gccagtacga atggtttttc tataatagat tcagaaaatg acatggaagg
     3001 aataatcaca ttatccagaa aacccaataa agcatgcttg gcttcttcac caaaaaacgg
     3061 attagctcca atacgacctc gcaaatattt tttaatgccg ggtaattcgt caatatgatg
     3121 gtcactcatc aagaaataaa tattacccat ggaatgatca aaaatataca cgggatgacc
     3181 tctgtcgcca aaaaactcta caactgcatt tatattgcgc atgacatgtt tggagtaatc
     3241 atcaacaggc aaatcattgg gtatagtacc ataatgcatt gccgcagcaa tatatttagt
     3301 agtcggaatc gtggtcagta aaccatcaaa tgagtttaaa ccaagaaatc catgtatgag
     3361 caagataacg ggaagtttct ctttcgcatt ttctttatag ggataagtta agtctaagcg
     3421 aatggttgtc gttccaaaac gagtgtcgcg cttaatttct atttgtggcc aggtaagtgc
     3481 ttcatcatca ataaaaacag tgagcctttc ttttacatta tcaataaacc ttataaaact
     3541 gggactttcg gttggtttgg ccgttgtgct gtaaagccat gccagtggtc ttcttttatc
     3601 tgattttgta gctaaaaagg caattcgttt tgtttgttgt agaaaataag gtgttgtcgt
     3661 tatttctttt tctttgttaa gcaagacaag tgtagaaaca tctctggatt caaaggcttt
     3721 tgagttacgc tcaataccgg caattctgcc ttttgggtta gatgctaaaa acgcatagtc
     3781 aaatcctgat ttattatttt ccctgagaaa gtttgtaagt gtagaattaa attcctccat
     3841 gtaagcttcc agatgctcat catcaatatt tcttcccaaa acctgttcgc gggttatcat
     3901 gcccgttctc accagtgtct tcagaaacgg acctgcatga aaaattccta ccagtcgttc
     3961 ctggcttttt aatggcgaat tgacaaagga atagtaaatt ttatgtgggt tttttatttt
     4021 tgtataattg tcaaaaatat attgataaac accagccgga gattgaccat gagataatcc
     4081 gaccagcaat ttttctccct tctctaatac tttatttgca taagctataa aatcttttcc
     4141 tacggcttca ttgaattctt ctttagattt aaaataacgg gtgtcggggg cattgtaggc
     4201 aggcagattt ccggtaaggt tcataaacag ttaactaaaa gtgagtatta aaattaagca
     4261 ataatgcaac gaacattcaa agacttgtat ttatgtcaat attaattttt tcatctttgc
     4321 catgtcgcag tttataagta attgatttag tttgagtaat aatttcccat gtttttgtta
     4381 ttttgcttat acaatactac aaaatgataa aacaatattc tttacagctt ccgccatttg
     4441 aaaagggact tcatctaatt accaaacaaa ttgtcttaca attgcaaaat ttaccggaaa
     4501 aaggtttgct gcatttgttc attctgcata cttcagcagg attgatggtt aatgaaaatg
     4561 cagatcctga tgtgcgtatt gacatggaaa attggtttaa cagaactgta aaagaaaatg
     4621 aaccttatta ccgtcatacg cttgaaggca gcgatgatat gcctgcgcat atcaaatccg
     4681 cactgaccgg aaattcactg acgataccca ttgttaatca taaactacat ttgggcacat
     4741 ggcaagggat ttatttatgt gaattcagaa accatggagg aagcagaaat atcatggcta
     4801 ctgtcatcgg ataaattttt taaagagtag catatctaaa tacttgaaaa ccgcttaggg
     4861 tttgtttcgc gcatcaagtt ataaatggtt tgaaagacat cttctgcatt tggtttgcaa
     4921 aaataatctc cgtcacttcc gaatgcccct ctgtgttctt tagcggtaag tgttacaggt
     4981 ggattatcca aataacggaa aatatgctgt ttttccatca cttgctgcaa catgtatgcc
     5041 gttgcgccac ccggaaaatc ttcatcaaaa aagaccactc tgtttgtttt ctttatcgat
     5101 tctcctatca caccataaat atcaaatggc atgagtgttt gcacatctat caattcgata
     5161 gaaataccta cccgctctaa attttcaagt gcttcttgtg caatacgtac acatgaacca
     5221 taggtgacta atgtaacatc gttgccttct ttcaatattt ccggcacgcc taatggcacg
     5281 gtgaaatctc cgggattttt gggcattttt tctttcagac gatacccgtt taaacattca
     5341 ataaccaatg ccggttcatc accacgcaaa agtaaattat aaaatcctgc tgcttgtgtc
     5401 atattccgag gtacgcataa atgcatgcct ctgagcgctc ccagtatcat ctgtatgggc
     5461 gatccggtat gccagatgcc ctctagccga tgccctctgg tacgtacaat cagcggagct
     5521 ttttgccctc cttttgttct gtaacttaaa caagccaaat catcagagag cggttgtaat
     5581 ccgtataaca aatagtcgag atattgtatt tctgcaatcg gccgcaagcc acgcatggct
     5641 aacccgatgc cttcgcccat aatacttgct tcacggatgc cggtatcata aatacgttca
     5701 actccgaatt tttcctgcat acctgcaaaa gcctgattaa catctccgat tttgcctaca
     5761 tcttcgccga atgccactat gcttttgtct cgttcaaaat tggcattaaa acaagcattt
     5821 aaaatctgat aaccacttac tatagatggc ttttgctcat attcagcttt aacaagaggc
     5881 aattttaatg gagaataagc tgaacctgta tgctgatatg atgaatatct ttcttcgttt
     5941 attaataatt gtgtttcgcg ccatttttta agagcagtga tatgcacatt gttttcatct
     6001 ttcaccgtga ttaatacatc gttcactact ttcattatat cgcgacgcaa tggatcgata
     6061 ctttgctgta agccgataac atattcctga agtagttctt tatgaacaga agtcaatgca
     6121 atcgcatttg tcatagcaat aacttcttta agttcttcta aaatcggccc atgaaatttc
     6181 gaccaggctt cttttttctt tccggctaca aaatttttgg cttctttttc taatgtattt
     6241 aattcatcat cagtagcaat cttactgtta ataatccagt ggcgaaattt taagatacag
     6301 tcaaaatctt tttcccattg caagcgttct gcagatttgt atcgttcatg agaaccggaa
     6361 gtagaatgac cttgcggctg agtcacttct tcaacatgaa tcaatgcagg gatatgtgta
     6421 cggcgggttt tgtcggctgc tgctttgtat gtagaaatta actgtggata attccatgct
     6481 tttaccacat aaatatcata tcccttgttt ttattgtcgc gctggaagcc ttttaaaacc
     6541 tgtgaaatac ttcctttcgt cgtctgcagt gatttaggta ctgaaatgcc gtaaccatca
     6601 tcccacactg aaacaattag cggtacttgc aaaacgccgg ctgcattgat gacttcccaa
     6661 aaaatccctt cagaagtgga ggcatcgcct atggtagcaa aacagatttc gttaccgttt
     6721 ttactgaaac cgttttctcc gatttgatct tgaagttctc tgtatttttt tgaagccata
     6781 gccaaaccca atgcccgcaa catttgtgcg gcagttggcg aattatcggc agctgaattt
     6841 ttttgatgga ttaaatcttt ccatgaaccg tcaggaaata aatttcgggt ggcaaagtgg
     6901 ttattcattt gtcgcccacc tgaatgcgga tcatcttcca aagagggatt ggcatagagt
     6961 tgtccaaaga gctgatcgac agaaacgata ccggttgcca gcgcaaaagt ttggtcgcga
     7021 taatatcccg aacggaaatc tccgttttca aaaactttag ctaatg
//
LOCUS       DAOQII010000123         6959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6030_length_6959_cov_5.551419, whole genome shotgun sequence.
ACCESSION   DAOQII010000123 DAOQII010000000
VERSION     DAOQII010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6030_length_6959_cov_5.551419"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..1382)
                     /locus_tag="PLF48_07055"
     CDS             complement(351..1382)
                     /locus_tag="PLF48_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49118.1"
                     /translation="MNPQKHNPENVGLESRKQDHIQLAFQSQVDSIYIDKRFSYEPLF
                     SEHPNKERQSFSFLGKQLQTPIWVSSMTGGTEKAYTINRNLAKACAKYKMGMGLGSCR
                     SILESNDRIQDFDFRDIIGSDLPFYANIGIAQLELFLLQNKLPVIDELVAKLSADGII
                     IHINPLQEWLQPEGDKIIQKPLDTLVDFLHKTKQKVIVKEVGQGFGKESLRALVQLPI
                     QALELAGHGGTNFSMLELLRSNEQRFTSFKQISQIGHSAEEMVTNLNDLKQELGDKFV
                     CENFIISGGIKNFLDGYYLMEKLNANSVYGQASELLKYAEKSFDALDEYLELQIAGLK
                     LAKAYFRVK"
     gene            complement(1463..2152)
                     /locus_tag="PLF48_07060"
     CDS             complement(1463..2152)
                     /locus_tag="PLF48_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNF49119.1"
                     /translation="MKKIVLSLATLFILSKSFAGDISEKSNLFGLLGGFSVNTMNYKE
                     NSDDKRGAKVGGLFGFAFEHRFKNVAAIEIQALYVNKGAQSKTDNAFFKTTNRLNFHS
                     VELPVLAKFYLGKKKIFNINVGGYASYSFVTQLRVKGENKVTGDKIDDKSENYSKSEN
                     NPKDVNGNRLFNVYDAGVIGGFEFISQKGFGAGARLSQGFIDFTNPKYIVDDDKKIWH
                     TGVQFYMTYKF"
     gene            complement(2285..2914)
                     /locus_tag="PLF48_07065"
     CDS             complement(2285..2914)
                     /locus_tag="PLF48_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNF49120.1"
                     /translation="MDGTISNPREGIFNAYRYAFGKMDLPIPDDEKLKQYVGPPLRKV
                     FGAYLNNNEAAIQAAITAYRHYYIDQQGMYENALFDGMFELLSGLNDYGITLSVATFK
                     GASVDKILDHFAISHFFPIVEYYNEEKGIVTKAKMIANILEYLQITDKNQVLMIGDRE
                     HDIFAAHETGVKSCAVTYGFGCVEELNMCKPDFMVNDVATLKQLVLPLA"
     gene            complement(2946..3398)
                     /locus_tag="PLF48_07070"
     CDS             complement(2946..3398)
                     /locus_tag="PLF48_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNF49121.1"
                     /translation="MSLDQNMNQELKEAMLSKNEPKVRTLKAIKSAFIIAKTEKGANG
                     EISAEQEIKILQKLFNQRKESLDIFTRENRPELAAKEKEEMEVIASYLPKQMSDEELK
                     AIILSTITETGASSIKDMGKVIGSVSKKVAGQAEGARISAMVKELLGA"
     gene            complement(3475..3687)
                     /locus_tag="PLF48_07075"
     CDS             complement(3475..3687)
                     /locus_tag="PLF48_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49122.1"
                     /translation="MFRLLEFIVLFLLAYKLINELFGRPRKVVHQDNSSSRKTVREEI
                     HHSSGQSATKHPSYDNAESIDFEEVK"
     gene            complement(3777..4850)
                     /locus_tag="PLF48_07080"
     CDS             complement(3777..4850)
                     /locus_tag="PLF48_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HNF49123.1"
                     /translation="MGKKLFLHWSVILPLMAIPLFVFTLIVQQAWLYIPLTFLLIIAV
                     LASVHHAEVIADKVGEPLGTLILALAVTIIEVSLVISLMIAGGKSTQTLARDTVFSAV
                     MIILTGIVGLCLLLGGAKYREQSFRRSGVNVALITLTAILVLTLVLPNFTTSVPGPQY
                     SAVQLIFVGIISLILYSAFVWVQTKLHRDYFTAETASANEEHEQIPTNAAFLSGGLLL
                     LSLVMVVIIAKKLSSPIELLLQQINAPQTLLGIIIAMVILLPEGLAAIKAARKDSLQT
                     SLNLALGSALASIGLTIPAVAFICVFMGLPVVLGIDSKSMLLMLLSLFTVALSFITGK
                     TTVMHGIVLLVIFSVYIFTVIVP"
     gene            complement(4923..5915)
                     /locus_tag="PLF48_07085"
     CDS             complement(4923..5915)
                     /locus_tag="PLF48_07085"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNF49124.1"
                     /translation="MKVAVVGATGLVGTEMLNVLLERKFPVSELIPVASERSAGKKIN
                     YNGNEYTVVTPQKAIEMKPAVALFSAGGNTSLEWAPKFAEAGITVIDNSSAWRMDPTK
                     PLIVPEINADILTKDDKIIANPNCSTIQMVVAINPLHKKYGIKRVVVSTYQSVTGTGK
                     KAVDQLMNERAGIEGEMAYKYKIDLNVIPQIDVFLDNGYTKEEMKMVKETCKIMRDDN
                     IKVTATTVRIPVMGGHSESVNIEFENDFDLSEVFQLLGNAPGVLVVDEPSSQLYPMPK
                     DAHGKDEVFVGRLRRDETQPNTLNMWIVADNLRKGAATNAIQIAEYLNAQGFLN"
     gene            6042..>6959
                     /locus_tag="PLF48_07090"
     CDS             6042..>6959
                     /locus_tag="PLF48_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNF49125.1"
                     /translation="MRKVYLLLWILLFSKLGHAQFNDQFTDGGITNNPSWTGNLTSFQ
                     VNPAKELQLNASLSGGIAYLVTGSTLTRSVEWNMYTRLQFNPSDNDYVKFYLFSTSND
                     LTASNQGYFLKIGRNGASDGLEFYRQDGTNEVLLKNMLVAQLAAQPELSIKVIKNNLG
                     KWVFYWKNAGAPTFTAIDSLTETTYTATNYFGLYCQYTGANATNFYFDDISAIQLPDV
                     ISDITPPVITAVNAVNATHVDVVFSEPVSNPTAGTVANYLLSPSITVTSTTLDVANPN
                     TVHLVLATPLASNTAYTLTVNNVQDTASNS"
BASE COUNT         2086 a         1424 c         1142 g         2307 t
ORIGIN      
        1 aaattgttgc tgaaccgctt catcttcatg ccagaagctt ttaatttctg cttttatttt
       61 ttcatcacca ccaaagtaat tttcgattag ccagtcaatt ttctggtctt ttgtgagctt
      121 ggaatagcct tcaattggtt tcatgcgatt tgtattttat aatatctttc ttgctgataa
      181 tcttcttctg atttttataa agcacctccc ccctgccccc tcctttgcaa ggagggggaa
      241 ttaaaagaag tggtttattt caaacttttt taaattttat ttatcacctc aacaacatta
      301 tctattactt cttcattctt aaatcttact aatattttgt acaaagcggt ttattttact
      361 ctgaaatatg cttttgccaa ttttaatcct gcaatttgca attccaaata ttcatccagg
      421 gcatcaaacg atttttctgc atacttcaac agctcacttg cttgtccata tacactgttg
      481 gcatttaatt tttccatcaa atagtagcca tctaaaaagt ttttaattcc tccggaaatg
      541 ataaaatttt cgcatacaaa tttatcgccc aactcttgtt tcaggtcgtt aaggtttgtt
      601 accatttctt ctgcactatg gccaatttgt gaaatttgtt taaatgaagt aaatctttgc
      661 tcgttgcttc gcaaaagttc gagcatggaa aaattagtgc cgccatgccc tgccaactcc
      721 agtgcttgga ttggcaattg taccagtgca cgcaaactct ccttaccgaa accttgtcca
      781 acttctttta ctataacttt ctgtttggtt ttgtgtaaga agtcgaccag tgtatccaat
      841 ggtttttgta ttattttatc accttcgggt tgcaaccatt cttgtaatgg attgatatga
      901 atgataattc catctgcact taatttggct acgagttcat ctattacagg caatttattt
      961 tgcagcaaga ataattccaa ttgcgcaata ccgatattgg cataaaaggg caaatcacta
     1021 ccgatgatat cgcgaaaatc aaaatcctga attctgtcat tgctttccaa gatagaacgg
     1081 caagatccca aacccatacc cattttatat ttggcacacg ctttcgcaag atttcggttg
     1141 atggtgtatg ccttttcagt gcctcctgtc atgcttgaca cccaaattgg agtttgaagt
     1201 tgtttaccta aaaatgagaa tgattgtctt tctttattag gatgctcaga aaaaagcggt
     1261 tcatacgaaa atcttttatc gatataaatg ctatctactt gtgactggaa tgccagctgg
     1321 atatgatcct gcttacgaga ctccaaacct acgttttccg gattatgttt ttgaggattc
     1381 actactacaa aagtaagtat aaaatcagcg cgaaataaaa aagccgccaa aaatggcggc
     1441 tacttatgat tatttaatac aattaaaatt tgtacgtcat ataaaactga acaccggtat
     1501 gccatatttt tttatcatca tccacaatgt atttcggatt tgtaaaatca ataaaacctt
     1561 gagacaaacg agctcctgct ccaaatcctt tttgagaaat aaattcaaat cctccgatta
     1621 cgcctgcatc ataaacatta aatagtctgt ttccatttac gtccttagga ttgttttccg
     1681 attttgaata gttttctgac ttatcatcta ttttatcacc ggtaacttta ttttcgcctt
     1741 tcactctaag ttgagttaca aatgaataag atgcatatcc acccacatta atattaaata
     1801 tcttcttttt gcccaaataa aacttagcca aaacaggaag ctctactgaa tggaaattca
     1861 atctgttggt tgttttgaaa aaagcattgt ctgtcttaga ctgtgctcct ttatttacat
     1921 ataatgcttg gatttcgata gctgctacgt tcttaaaacg atgctcgaaa gcaaagccaa
     1981 acagcccgcc tacttttgct cctcttttat cgtcagaatt ctctttgtag ttcatagtat
     2041 ttacactaaa accgccgagt agtccgaata agttactttt ttctgaaata tctcctgcaa
     2101 aacttttgct caagataaat aatgttgcta atgataatac gattttcttc ataaaataaa
     2161 ttttctttat ggtttcaaaa ttaataccaa atgtaggata attacaaata aaataagaaa
     2221 atacttagta accgttagtt tctattgagt attcggtaat attatttcct acagtatcta
     2281 gaaattatgc caatggcaaa acaagttgct ttagtgttgc tacatcattc accataaaat
     2341 cgggcttaca catattcagt tcctctacac acccaaatcc ataagtaacc gcacaagatt
     2401 taactcctgt ttcatgagca gcaaatatat catgctcacg atcgccaatc atcaatacct
     2461 gatttttatc tgttatttgc aaatactcca gaatattcgc tatcattttt gctttagtta
     2521 caataccctt ttcttcatta taatactcaa caatagggaa gaaatgcgaa attgcaaaat
     2581 gatccaaaat tttatcaact gacgccccct taaatgttgc aacagacaaa gtgattccat
     2641 aatcatttaa tccggacagt agctcaaaca ttccgtcaaa cagtgcattt tcgtacatac
     2701 cctgttggtc tatataatag tgtctgtaag ccgttatggc agcctgaata gcagcctcgt
     2761 tattatttag ataagcacca aagacctttc tcaaaggcgg ccccacatat tgtttcagct
     2821 tttcatcatc cggaataggt aagtccattt taccaaaagc ataccgataa gcattgaaaa
     2881 tgccttctct tggattggaa atagtcccat ccaaatcaaa aattagatgc tgaaatcgtc
     2941 ctttactatg cacccagtaa ttcttttacc atagcagaaa ttcgcgcccc ttctgcttgt
     3001 ccggctactt ttttgcttac gctacctatc acttttccca tatccttaat agaggatgca
     3061 ccagtttctg ttattgtaga caggataatt gctttcaact cttcatcgct catttgtttt
     3121 ggcaaataag aagcaattac ctccatttct tctttttctt ttgctgccaa ctccggacga
     3181 ttttcacggg taaaaatatc caatgattct tttcgttggt taaataactt ttgtaaaatt
     3241 tttatttcct gttcggcaga aatttctcca ttcgctcctt tttctgtctt agcaataata
     3301 aatgctgatt tgattgcttt taaagtacgt actttaggct catttttaga gagcatggct
     3361 tcttttaact cttggttcat gttttgatcg agactcataa ctattggttt tgcaatgtaa
     3421 aatttattgc tgcaaaatta taaaaaacaa agacatcaaa taaattaccc gaaattattt
     3481 tacctcctcg aaatcaatag attcagcatt atcgtaactg gggtgtttgg ttgcagactg
     3541 acctgaactg tgatggattt cttctcttac cgtttttcgg gaagatgaat tatcctgatg
     3601 cacgactttt cttggtctgc caaacagctc atttattagt ttatatgcta ataaaaataa
     3661 aacgatgaat tctaataagc gaaacatata caaatataca aagcaataac aaatttgtac
     3721 agcagaaagt tcaatagcac gctcttgctg attgcttcat ttagttctca acttccttag
     3781 ggaacaatta cggtaaagat ataaactgaa aagataacca gcaacactat gccatgcatc
     3841 actgtggttt tcccggtaat aaatgataag gcaactgtaa aaagcgataa cagcatcaac
     3901 aacattgatt tgctgtcaat acctaaaaca acgggcagtc ccatgaaaac acaaatgaat
     3961 gctacagcgg gaattgtgag tccgatgctg gcaagcgcag agcccaaagc aagattcaga
     4021 ctagtttgca agctgtcttt tcttgctgcc tttatggctg ctaatccttc gggaagtaaa
     4081 ataaccatgg ctattattat acccaacaat gtctgtggtg cattaatttg ttgtaaaagc
     4141 aattctatcg gcgacgataa ttttttagca attataacaa ccatcacaag ggataataag
     4201 agcaatcctc cgctcaaaaa agctgcattt gtcgggattt gctcatgctc ttcatttgca
     4261 gatgctgttt cggcagtaaa ataatcccga tgtaatttgg tttgcaccca aacgaaagcg
     4321 ctgtataaaa tcagtgaaat tatacctaca aaaatgagct gcactgctga atattgagga
     4381 ccgggaaccg atgttgtaaa atttggcaag acaagtgtaa gcactaatat agccgttagg
     4441 gtaattaatg caacatttac gcccgaacgc ctgaaagact gttctctata ttttgctccg
     4501 cctaacaata aacacagccc gacaataccc gttaaaatta tcattactgc tgaaaaaacc
     4561 gtatctcttg caagtgtttg cgtgcttttt ccaccggcta tcatcagcga aataaccaat
     4621 gaaacctcta taatggtaac ggcaagtgcc aaaataagcg tacccagagg ttcacctact
     4681 ttgtcagcaa ttacttctgc atggtgtacg gatgccaata ccgcaataat aagcagaaat
     4741 gttaaaggta tgtacaacca cgcttgttgg actattaatg taaatacaaa caggggtatt
     4801 gccatcaaag gcaatataac tgaccagtgt aaaaagagtt ttttgcccat ataaaaatga
     4861 ttgcagtatc tttttactaa agatatctaa aaagagacaa actaactaaa cgctaccggg
     4921 ttttagttta aaaagccttg tgcatttaaa tattcagcta tttgtatagc attagttgct
     4981 gcgcctttgc gtaaattatc tgccactatc cacatgttca aggtattcgg ctgtgtttca
     5041 tccctgcgaa gtctgcctac aaatacttca tctttgccat gcgcatcttt tggcatcggg
     5101 tataattgag acgaaggctc gtctacaact aatacgcccg gggcattgcc cagcagctgg
     5161 aatacttcag ataaatcgaa atcattttca aactctatat tcacactctc gctatgcccg
     5221 cccatcaccg gaattcgaac tgtagttgcc gttaccttaa tattgtcatc gcgcataatt
     5281 ttgcaggttt ctttcaccat tttcatttct tctttggtgt aaccattatc taaaaataca
     5341 tcgatttgag gaattacatt caagtcaatt ttgtatttat atgccatttc accttctata
     5401 cctgcacgct cgttcatcag ttggtcaaca gctttttttc ctgtaccggt tactgactgg
     5461 taagtagaca ccacaacacg cttaattccg tatttcttat gtaaaggatt gatagcaaca
     5521 accatctgga tagttgaaca gtttgggttg gcaataattt tatcgtcttt agttaagata
     5581 tctgcattaa tttcaggaac gataagcggt ttggtagggt ccatacgcca tgctgacgaa
     5641 ttatcaataa cggtaatacc cgcttcggca aatttcggtg cccattccaa ggatgtattt
     5701 cctccggctg aaaaaagtgc cacagccggt ttcatttcga ttgctttttg tggcgtaaca
     5761 accgtgtatt cattgccatt atagttgatt ttctttccag cggaacgctc agaggctaca
     5821 ggaatcaatt ctgaaaccgg aaacttgcgt tcaagcaaaa cattcagcat ttcggtgcct
     5881 actaatccgg ttgctccaac tactgctact ttcatatttg gtattttttt acaaaaatag
     5941 atactctgct taacttttct gtttaattta atattacgaa acaaccattc gttgtatttt
     6001 accgactaaa taatggaaga agtgattaaa tttattagtt gatgagaaag gtatacctgt
     6061 tattatggat tttattattt tctaaattgg gtcatgccca gtttaatgac caatttactg
     6121 atggtggtat taccaataat ccgtcatgga caggcaactt aacttcgttt caggtgaatc
     6181 cggctaaaga attacagcta aacgcctcat taagtggtgg cattgcatat ttagtaacag
     6241 gcagtacact tactcgcagt gtggagtgga atatgtacac ccggctccag tttaatcctt
     6301 ctgacaatga ttacgttaaa ttttatttat tttccacctc taatgactta actgcatcta
     6361 atcagggcta tttcttaaaa attggaagaa atggtgcatc tgacggactt gagttttacc
     6421 gtcaggatgg cacaaacgaa gttttattaa aaaacatgtt agttgctcag ttagccgctc
     6481 aacctgaatt atctattaaa gtaattaaaa acaatttggg taaatgggtg ttttattgga
     6541 aaaatgctgg agctcctact tttaccgcta ttgacagctt aacagaaacc acttatactg
     6601 ctaccaatta ttttggttta tattgccaat atacaggggc taatgctact aatttttatt
     6661 ttgatgatat cagcgccatt caattaccgg atgttatttc agacattacc ccacctgtaa
     6721 ttactgctgt aaatgccgtt aatgccacac atgttgatgt cgttttttct gagccggtaa
     6781 gcaacccgac tgccggtacg gttgcaaatt atttattaag cccttctata actgtcacct
     6841 ctactacgct ggatgtggca aatccgaata cggttcactt agtgcttgca acacctttgg
     6901 catcaaacac cgcctacaca ctgaccgtaa ataatgttca ggatacggca tccaacagc
//
LOCUS       DAOQII010000124         6864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6142_length_6864_cov_6.684095, whole genome shotgun sequence.
ACCESSION   DAOQII010000124 DAOQII010000000
VERSION     DAOQII010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6142_length_6864_cov_6.684095"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..643
                     /locus_tag="PLF48_07095"
     CDS             86..643
                     /locus_tag="PLF48_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49126.1"
                     /translation="MSDRIKIIEKNDLLEIRIALKKNTAALVAMILATLAWVFVLFVF
                     INITLRLHFFWYKAGMILAILSWFALGMAGASFFIWLFWGRERIIVSKDYFITDKPLV
                     FFYRRNFYPISEVAQLRSDKEVYQVNRNGNWVEESRTVLKFETPDKQVIFARGIEKEE
                     AEFILLQLAKCSELKKEQFAVEQKF"
     gene            complement(675..1796)
                     /gene="rffA"
                     /locus_tag="PLF48_07100"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(675..1796)
                     /gene="rffA"
                     /locus_tag="PLF48_07100"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNF49127.1"
                     /translation="MLIPFNRPLLTGKELTCIEQAVRYGKISGDGYFTNLCEQFFENK
                     YDFSKCLLTASCTDALEMCALLLDIQPDDEVILPTYTFVSMANAFYMRSAKLIFADSR
                     SDHPGIDEQKIETLITPKTKAILVMHYGGVACDMDVIRTLADKFNLAVVEDAAHAIDS
                     YYKDKPLGSFGDLATFSFHETKNITCGEGGMLVINRKDLCERAEIIREKGTNRKQFIN
                     GKTEKYNWVDIGSSYLMSDINAAYLYAQLEEIENIQTKRLKIWNQYYENLKSIKDKIG
                     LPYVPDYATNNAHLFYMVCQSKTERDDLIEHLRSKNIYAVFHYQNLHQSPFFSSQYHG
                     KELPNAQVYNDCLLRLPLFFDLSETDLDYISKAVISFFK"
     gene            complement(1810..2754)
                     /locus_tag="PLF48_07105"
     CDS             complement(1810..2754)
                     /locus_tag="PLF48_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49128.1"
                     /translation="MVSCSSCKKNKNNQEQFNEQKKEAYDMLPPEQQVIIDEVTHWNA
                     LHDSTNIDSLEQFYADQVLFFKKVRSKKDVIEMKKYRMKHADTYRERIIGRIGIYAAD
                     TGDYKCEFLKLVTINEKPSVFHSYLIFKKMQDNTFRIIVESDKESDNKKTFIYDTLFT
                     AHAMSESLSQGDFNGDGTKETLIIQAPEQDTLGNYSSTTTKITFDNLELPEIILQNTV
                     GVNVLNENDVDGDGSDDFSVVIKKPNGEYGNVILYSFKRGNWVKLADFRALNSEVNKA
                     RQNLIDFAGSGNIKIREAQKTIDGRDTVIVKTISTWGD"
     gene            3339..3617
                     /locus_tag="PLF48_07110"
     CDS             3339..3617
                     /locus_tag="PLF48_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49129.1"
                     /translation="MAKKTASAKPAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAAPKK
                     AAAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAAKKK"
     gene            complement(3683..4300)
                     /locus_tag="PLF48_07115"
     CDS             complement(3683..4300)
                     /locus_tag="PLF48_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNF49130.1"
                     /translation="MSLIYIIDLLGTFVFTISGALAASDKEHHHDLFSVFFTGFITAI
                     GGGTIRDIILGAYPVCWIADGNYLLAVFAGVLFTLLTRKHIIRMSRTLSLFDTLGMAI
                     YVIMGVEKSLSLHVSVYAAVILGMISGIFGGVTRDTLLNNIPMIFRKELYATPCLLGA
                     LLYVLLLKAGMEAQVNFIVSVLFIFIFRILAVKYKWSLPTLTFRE"
     gene            complement(4316..4801)
                     /locus_tag="PLF48_07120"
     CDS             complement(4316..4801)
                     /locus_tag="PLF48_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF49131.1"
                     /translation="MQIRTLTLLDTFTETRKLTFYFLNKLTDVQKEAEPLVNGITLNS
                     AHWNMAHIAWAEYFLIHQAFGVNEIKSDWFDLVKIGSPKCKATDLPALTEVLETLTNV
                     HQHTKQFLKEVKDNQLEEDNLIDLTISGKKSKGMMLIHAIRHEGIHAGQIDWLAKITA
                     K"
     gene            complement(4869..5321)
                     /locus_tag="PLF48_07125"
     CDS             complement(4869..5321)
                     /locus_tag="PLF48_07125"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNF49132.1"
                     /translation="MKILMVCLGNICRSPLAEGIMKNKIKEKGLDWYVDSAGTGKWHL
                     GAAPDRRSIEVAQKHGIDISAQRARTIRSSDYEEFDLIFAMDIQNLKDLRGWALDKAE
                     ENKISLIMNEVYPGENISVPDPYFNDDGFEIVYQMLDKACDKIIERFQ"
     gene            5387..6208
                     /locus_tag="PLF48_07130"
     CDS             5387..6208
                     /locus_tag="PLF48_07130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNF49133.1"
                     /translation="MRISISAIILFLMLANAAFSKTKWQQTASGMQYKVAVTDNSKPM
                     PMYGDFIRMHLVKYDHKGKEIFNTRIFDKADGVEMELKHKPNEKEVTEIFTVMHEGDS
                     AFVKVVEKKGRSRHTFYYRLYLYDVERREVYFRQKDIQKQQQFYVDSISIQQYLRQNG
                     LTDAQPDGNGNYFIRSIIAPQKQIQAGDTVVMHYIGKFLDNTAFDNSIDRKQPFTFKV
                     GAKQVIDGLDKSIRQFNFGDKGIIFIPSKNAYGDNDNNGIPANSVLIFEVEIAAK"
     gene            6283..6726
                     /locus_tag="PLF48_07135"
     CDS             6283..6726
                     /locus_tag="PLF48_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HNF49134.1"
                     /translation="MFWKEVKTLKSTDFKRLTGVTNSTFQLLVEAVNEYDTKVRKRGR
                     TGRKSHLITEDKLLLTLMYLRECRTMFHIGVAYGLSESNVCRTIKFVEQALINHPSLQ
                     LPGKRVLTQSDNTFEVVLIDVAETPIERPKKNKENTILAKRKSTR"
BASE COUNT         2306 a         1254 c         1177 g         2127 t
ORIGIN      
        1 tatttggatg tgaaaaaaca aactttgcca agatagaaca agcttaataa gcttgtttct
       61 gtcttactcc agcttaacta acaggatgtc agatcgtata aaaattatag aaaaaaatga
      121 cctgcttgaa atccgcatag cactcaagaa aaatacagcg gctttagtgg ctatgatatt
      181 ggctacgctt gcctgggttt ttgtgttatt tgtatttatt aatatcacgc tgcggcttca
      241 ttttttttgg tataaagccg gaatgatatt ggccatctta tcatggtttg cgttaggaat
      301 ggcaggtgcc agtttcttta tctggttgtt ttggggtaga gagcgaatta ttgtcagcaa
      361 agattatttt attacagaca agccattggt gtttttttac agacgcaatt tttatcccat
      421 aagtgaagtt gcgcaattac ggagtgataa ggaagtatat caggtaaacc gcaatggtaa
      481 ctgggtggag gaaagcagaa cggtactaaa atttgagacc cctgacaaac aagtcatatt
      541 tgcccgtggc attgaaaaag aagaggcaga atttatactc ttgcaattag caaaatgttc
      601 agaattaaag aaagaacaat ttgcagtaga acaaaaattt taagtgggat acaaaatact
      661 gccaaaggca tattttactt aaaaaaagaa ataactgctt tactgatgta gtccaaatcg
      721 gtttcagata aatcaaaaaa caatggtagc cgaaggagac aatcattata tacctgtgca
      781 tttggcaatt ctttgccgtg gtattgagaa ctaaaaaacg gactttgatg aagattctga
      841 taatgaaaaa cagcataaat gtttttactt ctcagatgct ctattaagtc atctcgttcc
      901 gtttttgact gacaaaccat ataaaataaa tgcgcattat tagtcgcata atccggtacg
      961 taaggcaatc ctattttatc cttgatactt tttagatttt catagtactg attccatatt
     1021 ttcaatcttt tagtttggat gttttctatt tcttccagtt gtgcatataa atacgcagca
     1081 ttaatatcag acatcagata agaggaacca atatctaccc aattgtattt ttctgttttt
     1141 ccgttgatga attgtttccg gttggtgcct ttctcacgaa ttatctccgc tcgttcgcac
     1201 aagtcttttc tgtttataac caacatgcca ccttctccgc acgtaatatt ttttgtttca
     1261 tgaaaagaga aagtagctaa atcgccaaaa cttccaagag gtttgtcttt gtaataactg
     1321 tcaatagcat gtgccgcatc ttcaacaact gcaagattaa atttatcagc caatgtacgt
     1381 ataacatcca tatcacaggc tacaccacca taatgcatga ctaatattgc ttttgttttt
     1441 ggggtaataa gtgtttctat tttttgttca tcaattccgg gatgatcgga acggctgtcg
     1501 gcaaaaatta attttgcact gcgcatataa aatgcgtttg ccatagatac aaaagtataa
     1561 gtgggcaata tgacttcatc atccggctga atatccaaaa gcaaggcaca catctctaag
     1621 gcatctgtac aagaagcggt cagtaagcac ttggagaagt catatttgtt ttcaaaaaac
     1681 tgctcgcata aattagtaaa atatccgtcg cctgaaatct taccatacct gacggcttgc
     1741 tcaatacaag tcaactcttt acctgtaagt aatggtctgt taaaggggat aagcatgctt
     1801 tagtgttttt taatcgcccc aggtagatat agtttttaca attactgtat ctcgcccatc
     1861 aatagttttt tgcgcttcac gaatttttat atttccgctt cctgcaaagt caattaagtt
     1921 ttgtctggct ttatttactt cgctgttcaa tgccctaaaa tcagcaagtt ttacccaatt
     1981 gcctcttttg aaactataga gaattacatt gccatattct ccattcggtt tttttattac
     2041 tacggaaaaa tcatccgagc cgtcgccgtc tacatcattc tcgttgagca catttacacc
     2101 tacagtattt tgtaaaataa tctcgggcaa ttcaaggtta tcaaaagtaa tttttgttgt
     2161 tgtggaagaa tagttcccaa gagtgtcttg ctccggtgct tgtataatta gagtttcttt
     2221 tgtgccatca ccattaaaat caccttgaga aagactctcg ctcatagcat gtgcagtaaa
     2281 taaggtatca taaataaatg tctttttgtt atcagactcc ttatcacttt ctacaataat
     2341 gcgaaatgta ttatcctgca tctttttaaa aatcagataa gaatggaata cactcggttt
     2401 ttcgttaata gtaacgagct ttaaaaattc acatttataa tctccggtat cggctgcgta
     2461 aattccaatt ctgccaataa tgcgctcgcg ataagtatcc gcatgtttca ttcggtattt
     2521 tttcatttcg ataacatctt tttttgaccg aactttttta aagaacaata cttgatcagc
     2581 ataaaactgt tcaaggctgt caatatttgt cgaatcatgc aacgcattcc agtgcgtaac
     2641 ttcatcaata attacttgct gttccggagg aagcatatca tacgcttctt tcttttgctc
     2701 gttaaactgt tcttgattgt ttttattttt tttacacgat gaacaagaaa ccatgtaaaa
     2761 cattgcggcg aaaaaaatag tgaaatacca aactgctctc ataaaaataa ttacaagttt
     2821 aaagatacaa attctataca aacagcaatt gttattaatt ataaactagg atatctgctg
     2881 attctgaaaa aaaattgtca acacttcaca atagctatcc atatattttc tgttccgttt
     2941 tataaaaata tcagtgtggt tttttcattt tttattttgt aaaagcactt tcaattaatt
     3001 aacgcgtgtt aagcagcaaa aacaaattgc tccgaaatac caataaacac taattgaccc
     3061 acactatggt tgtttttata aaaatgtttt cattcatgca aataaaattt tatctaatac
     3121 agcttaataa tttttgtttg tgtgaatcat gtaattaatg ctttactttt ttccacatta
     3181 tcaacatata tgaataactc aagcgttgca tgcaattaaa gattgcatta tttttatgtt
     3241 gcagaaaatt taatagtgat ttcaatctgt aaaaaaaata aaatcgctga cagtaaagtt
     3301 tcgactgttg aaaacgaaac aaaataaaaa ataaaaaaat ggcaaagaaa acagcaagtg
     3361 ctaagccggc tcctaaaaaa gcagcagcac ctaaaaaggc agcagctcct aaaaaggcag
     3421 cagctcctaa aaaagcagcg gctcctaaaa aagcagcggc tcctaaaaaa gcagcagctc
     3481 ctaaaaaagc agcggctcct aaaaaagcag cggctcctaa aaaagcagca gctcctaaaa
     3541 aagcagcagc tcctaaaaaa gcagcagctc ctaaaaaagc agcagctcct aaaaaagcag
     3601 cggctaaaaa aaagtaatag ttcagaaact aatcaaaaaa taagcccggt attttatatc
     3661 gggcttattt tattgattcg aattattctc tgaatgtgag tgtaggcaaa ctccatttat
     3721 actttacagc gagtattcga aagataaaaa tgaacagtac ggaaacaatg aagttaactt
     3781 gcgcttccat ccctgccttt agtaaaagca catagagtaa agctccaagc aaacatggtg
     3841 tagcgtacaa ttcttttctg aaaatcatcg gaatattatt gagcaaagta tcacgggtaa
     3901 caccaccgaa tattcctgaa atcatcccta gtattacagc agcatatact gaaacatgca
     3961 aggataaact tttttctaca cccataatta cataaatcgc cattcccaag gtatcaaata
     4021 aactgagcgt gcgcgacatc cgaataatat gtttacgagt caatagggta aacaatactc
     4081 cggcaaaaac agctaacaaa taattaccat ctgcaatcca gcaaaccgga tatgcaccga
     4141 gtatgatatc gcgaatagta cctcctccaa tggcagttat aaaaccggta aagaaaacac
     4201 tgaataaatc gtgatgatgt tctttatcac tcgcagccag tgctccggat atggtaaata
     4261 caaaagtacc cagcaaatca ataatgtata tcaacgacat aactttagga ttggtttatt
     4321 tagctgtgat ttttgcaagc cagtcaatct gcccggcatg tatgccttcg tgtcttattg
     4381 catgtatcag catcatgcct ttgcttttct tgccgctaat ggtaaggtca atgagattgt
     4441 cttcctcgag ctgattatct tttacttctt taagaaattg tttagtgtgc tgatgcacat
     4501 tagttaaggt ttccaatact tccgttaaag ccggtaagtc tgtggccttg cattttggcg
     4561 aaccaatttt tactaaatca aaccagtctg attttatttc attgacgcca aatgcctgat
     4621 gaatcagaaa atactcagcc caggcaatat gtgccatatt ccagtgtgca gagttaagtg
     4681 tgattccgtt tactaagggt tctgcttctt tctgtacatc cgttaattta tttagaaaat
     4741 agaaagtaag cttgcgtgtt tctgtaaaag tatccagtaa ggtgagggta cgtatttgca
     4801 tttttctttt aaagataata aatcaattgc ttactgtaaa agaatacagg agtgcaaaaa
     4861 cggagctgtt attgaaatct ttcaattatt ttatcacatg ctttgtcgag catttgatag
     4921 acaatctcaa atccgtcatc attgaaatac ggatccggaa cggaaatgtt ttcgccggga
     4981 taaacttcat tcattatcaa actgatttta ttttcttctg ctttatccag tgcccagcct
     5041 cttaaatctt ttaagttctg aatgtccatg gcgaaaatca aatcaaattc ttcgtagtcg
     5101 ctgctgcgga tagttcttgc tctttgtgcc gaaatatcaa tgccatgttt ttgtgcaacc
     5161 tcaatagaac gcctgtcggg agctgcacct aaatgccatt taccagtacc agcgctgtct
     5221 acataccagt ctaatccttt ttcttttatc ttgtttttca tgataccctc agccaaaggc
     5281 gagcgacaaa tattacccaa acaaaccatc aaaattttca tgtactaaaa ataaaactat
     5341 aaagcctact tttacaaaaa agtacggttt ttgatattaa aataaaatgc ggatttcaat
     5401 aagtgctata atccttttcc tgatgctggc aaatgctgct ttttccaaaa ctaaatggca
     5461 gcaaacagcc tccggtatgc agtataaagt agctgttaca gataatagta aacctatgcc
     5521 catgtatggc gattttatac ggatgcattt ggtaaaatat gaccataaag gcaaggaaat
     5581 atttaacacc cgaatttttg ataaggccga tggtgtggaa atggaactaa agcataaacc
     5641 caatgaaaaa gaagttactg aaatttttac tgtaatgcat gaaggagatt ctgcatttgt
     5701 aaaagtggtt gaaaaaaaag gtagatccag acatacattt tattaccggc tatacttgta
     5761 cgatgtagaa cgaagagagg tttattttcg gcaaaaagat atacagaaac aacagcagtt
     5821 ttatgttgat tcaatttcca tacaacaata tcttcgccag aatggcttaa ctgatgcaca
     5881 acctgatgga aatggaaatt attttattcg cagcattata gcaccacaaa agcaaataca
     5941 agcaggagat acggtagtga tgcactacat cggtaagttt ctggataata ctgcttttga
     6001 taattctatc gatcgcaaac aaccctttac gtttaaagta ggggctaaac aagtaattga
     6061 cgggttggac aaatcaatac ggcaattcaa ttttggcgac aaaggaatca tctttattcc
     6121 cagtaaaaat gcctatggcg acaatgacaa taacggcatt cctgccaatt ctgttttaat
     6181 atttgaagta gagatagccg ctaaataata gacctcttgc gaaattcaga tttgtttaaa
     6241 agtctttatt aaaatattag gcattattta ttaaagttgc agatgttttg gaaagaagta
     6301 aagacactca aatcgacaga ttttaaacga ttaacaggcg ttacaaattc tacctttcaa
     6361 ttattagtag aagcggttaa tgaatatgat acaaaagtac gtaaacgagg tagaacaggc
     6421 aggaaatcac atttaattac agaggataaa ctattactga ccttaatgta tctaagagaa
     6481 tgtcgtacta tgtttcatat tggtgttgca tacggcttaa gtgaaagtaa tgtttgcaga
     6541 accataaaat tcgtggaaca agccttaatc aaccatccat cgttacagtt accgggcaaa
     6601 cgtgtattga cacaatcaga caataccttt gaggtcgtat taatagatgt agcagagaca
     6661 cccattgaac gacctaaaaa aaacaaagaa aatactattc tggcaaaaag aaaaagcaca
     6721 cgttaaaaac acaagtagtg gcagataaac aatcacgaag aataatttgc atacacaata
     6781 gtataggaaa aaatcatgat tttaagcttt ttaaaaacag cagaatattt atgcatccaa
     6841 acatcaaagc acaggtagat actg
//
LOCUS       DAOQII010000125         6835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6183_length_6835_cov_5.273009, whole genome shotgun sequence.
ACCESSION   DAOQII010000125 DAOQII010000000
VERSION     DAOQII010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_6835_cov_5.273009"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLF48_07140"
     CDS             complement(<1..777)
                     /locus_tag="PLF48_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNF49135.1"
                     /translation="MKYFILAGEPSGDEYAANLANAIRMKDTHAEIVGVGGKYMQNAG
                     IELLFGLDRLAFMGFVEVAKHLFTIRKNFKEIKSCLIQYKPDVVILVDYPGFNLRMAK
                     WCKLIGFKVVYYISPKIWAWKESRVEVIKKYVDKMLCIFPFELDFYKKHGYTNAVYVG
                     NPLEIKEQTPIDEIKTKNIIALLPGSRKQEIKRLMPVFIELAQLCSDKQFIIAAMSRM
                     KDEYQAFIPLPQNMQLVFDNTENVLNTTALAVVCSGTATLQ"
     gene            complement(788..1102)
                     /locus_tag="PLF48_07145"
     CDS             complement(788..1102)
                     /locus_tag="PLF48_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49136.1"
                     /translation="MNESQQTFLAKTDKIIYGFILGLILPLIMFYVYYALKYNQIPWE
                     DYVTQVKDISVLPKIIRICTFVNLPVLLAFNFTKQFNICIGLFAASFIYITFMFLVRY
                     IV"
     gene            complement(1108..1884)
                     /gene="surE"
                     /locus_tag="PLF48_07150"
     CDS             complement(1108..1884)
                     /gene="surE"
                     /locus_tag="PLF48_07150"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNF49137.1"
                     /translation="MDRPVILVTNDDGIIAPGIKALIEVAQKFGDVTVVAPDSPQSGM
                     GHAITIHEPLRMQKVELFGSIPAYQCSGTPVDCVKIAIDKILHRKPDLCVSGINHGSN
                     AAINVIYSGTMSAAMEAAIDGIPAIGFSLLDFSFTADFEPAKKYIEKIIREVFNNGLP
                     KDCLLNVNIPAVAAEEIQGMRICRQANARWVEEFDERKDPNGKNYYWLTGKFVNNDQG
                     EDTDVWALENNYVSIVPVEFDLTHHYGISFINKNWNLHEN"
     gene            complement(2145..3242)
                     /locus_tag="PLF48_07155"
     CDS             complement(2145..3242)
                     /locus_tag="PLF48_07155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF49138.1"
                     /translation="MNYISLENKNSNNEKKRFQELDALRGIAAVLVVFFHFTVGKNGY
                     DRYFKIGITGVDLFFIISGFVIFMSLQHINKSIDFVINRVSRLYPTYWASVSFTFFLF
                     TVYAIYKHTFSYKDIIIYMGNLTMFQYYLNIPDLDGPYWTMIIEMVFYISILLLFQFK
                     LLKYLNIIYVITCVTTVILTRFFWTSEWVRLLLKWIPLLQFLPLFFAGTIFYNLYTDS
                     GNTKKNYILILFCMICQLLLFPYVGRSSVILSQYSYSIILTVYFLLFVLFVNRKLGFI
                     VNRTTLFFGKISFALYLIHQYISLGFIIPSFHNQMHINFWVTCCLIDLPIIVAIATLI
                     TYKIDIPYSKKMKEKLRKLTNSRAQESINTL"
     gene            complement(3768..4811)
                     /gene="ilvC"
                     /locus_tag="PLF48_07160"
     CDS             complement(3768..4811)
                     /gene="ilvC"
                     /locus_tag="PLF48_07160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNF49139.1"
                     /translation="MAKMNFGGVEENVVTREEFPLSKAKEVLKDEVIAVIGYGVQGPG
                     QALNLRDNGFNVIVGQRRPSKSWDKAEKDGFVAGQTLFEPEEALEKGTIIMYLLSDAA
                     QIEMWPLVKKHLTAGKALYFSHGFGITYKERTGIVPPADVDVILTAPKGSGTSLRRLF
                     LQGTGLNASYAIFQDATRRAFDRTIALGIGIGSGYLFETTFQKEVYSDLTGERGVLMG
                     ALAGIMEAQYQVLRDNGHSPSEAFNETVEELTESLIKLVSENGMDWMYGNCSTTAQRG
                     ALDWKGPFREAVLPVFKELYSKVAAGEEAQRSIDTNSQPDYREKLEEELRQLRESEMW
                     QTGKIVRSLRPGK"
     gene            complement(4928..5173)
                     /locus_tag="PLF48_07165"
     CDS             complement(4928..5173)
                     /locus_tag="PLF48_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49140.1"
                     /translation="MKKFKIEFISLNQPVLIGRFMLIFSRRRIDVTDFSFTKINDGEG
                     KFTIEFIADEWQAENLKKQVQRQIDVYESTLLEMEND"
     gene            complement(5385..6044)
                     /locus_tag="PLF48_07170"
     CDS             complement(5385..6044)
                     /locus_tag="PLF48_07170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF49141.1"
                     /translation="MHEHLATYYHERANEYEKVYQIKDEQEDLLTAAHYFQNTFTEKS
                     IIEIACGTGYWTEQISKTASSIFSTDINHSLIDIAKKRTYFSPMQFEAADMYKIDTAH
                     KYDGLFAGFIWSHILIQDLDSFILNMSKLITKEGDLVFIDSKQVEGGVHAMENISRID
                     LYGNTFQTRKLENGKQFEVLKNFPDHSFLINKLEGISTDIRILDLKHYWILHAKNNAF
                     D"
     gene            complement(6091..>6835)
                     /locus_tag="PLF48_07175"
     CDS             complement(6091..>6835)
                     /locus_tag="PLF48_07175"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNF49142.1"
                     /translation="NNMLFSGLESVAHNINHKNPLIQLFEFGKTYTKTGNAAYQEENH
                     LALFLSGTVVSPNWNTSEKKADFYYVKALLESLLASIGVLKFETENAENEIFEYASTI
                     LCNGKTIAIFGKVNASLNKLCAIKNDVFYADVFWDEVLTITKKQEVQYKEISKFPAVR
                     RDLALLLDRSVSFAQVKNIAEKTGKKLLTDIELFDIYEDEKIGKDKKSYAVSFTFKDT
                     QKTLQDTEIEQVMAKLITAYQTELSAQIR"
BASE COUNT         2203 a         1170 c         1077 g         2385 t
ORIGIN      
        1 ttgtaaggta gcagtaccac tgcaaacaac ggccaatgca gtagtattta atacattttc
       61 ggtattgtca aagaccagtt gcatgttttg tggtaatgga atgaaagctt gatattcatc
      121 tttcatacgg ctcattgcag ctatgataaa ctgtttatca gaacataatt gtgccaattc
      181 aataaagaca ggcataagcc gttttatttc ctgcttgcga ctgccgggta aaagtgcaat
      241 aatattctta gttttaattt catctatagg tgtctgctct tttatttcca gaggattacc
      301 tacatataca gcattcgtat agccgtgttt tttgtaaaaa tccagctcaa acggaaaaat
      361 acagagcatt ttatccacgt atttttttat tacctccacc cgactttctt tccatgccca
      421 aattttagga gaaatgtaat acactacttt aaagccaatt agcttgcacc attttgccat
      481 gcgcaaatta aatcctggat agtcaactaa aatgactaca tcaggtttat attgtattaa
      541 acaacttttt atctctttaa aattttttct aattgtaaac aaatgtttgg ctacttcaac
      601 aaaacccata aaagccaggc gatctaaacc aaataaaagt tctattccgg cattttgcat
      661 atactttccg cctacaccca caatctctgc atgcgtatct ttcattcgga tagcattagc
      721 cagatttgca gcgtattcat cgccggaagg ttcgcctgcc agtataaaat atttcatttt
      781 taatttatta cacgatgtag cgaactaaaa acatgaatgt aatataaata aaagaagcag
      841 caaacaatcc tatacagata ttaaattgtt tggtgaaatt aaaggccaat aaaacaggaa
      901 gattgacaaa tgtacaaatg cggattattt tgggtaatac actaatgtct tttacttgag
      961 ttacataatc ttcccatgga atttgattgt atttcaatgc gtaatagaca taaaacataa
     1021 ttaagggtaa gattaatcct aaaataaaac catagataat tttatccgtc tttgctaaaa
     1081 aagtttgttg agactcgttc ataattttta attttcgtgt aaattccaat ttttattgat
     1141 aaaggaaata ccgtaatgat gcgttaagtc aaactcaacc ggcacgatag agacgtaatt
     1201 attttccagt gcccatacgt cagtatcttc cccttgatcg ttgtttacaa atttgccagt
     1261 aagccaataa taatttttgc cgttggggtc tttgcgctca tcaaattctt ccacccagcg
     1321 cgcattggct tgccggcaga tacgcatacc ttgtatttct tcagctgcaa cggcaggtat
     1381 atttacattc aataaacaat ctttaggcag accgttgttg aatacttcac ggataatttt
     1441 ttcaatatat tttttagcag gctcaaaatc tgccgtaaaa gaaaaatcga gtaatgaaaa
     1501 acctattgca ggaataccgt caatggctgc ttccatggct gccgacatcg tgccggagta
     1561 aatgacattg attgcagcat tagagccgtg attgatacca gaaacacata aatccggttt
     1621 gcggtgcaga attttatcaa tagcaatttt cacacagtca actggtgtgc cgctgcattg
     1681 ataagcagga atactgccaa aaagttcaac tttttgcatg cgaagaggtt catgtatcgt
     1741 aatggcatgt cccattccgg actgtgggct atcgggggca accacagtaa catcaccaaa
     1801 tttttgcgca acttcaatta acgcttttat tccgggagca attataccat cgtcatttgt
     1861 aactaagatt acaggtctgt ccatggtgta aaggtatgga atttgcaggg caacaacaag
     1921 ttgcataaaa gagaagccgt gattaatgtt tatttattga atttatcatt tagcaaatga
     1981 aacttatgat ttctgattta tatcaatgca ttttcatctg aaaagtataa ggatattcat
     2041 ctgtacttgt aaataacact atgtgataaa acatgcgtat atataataat taattaagct
     2101 atcgtttact tctattaaaa attagatgtt gtaaagtgtt gatattacaa tgtgttaatt
     2161 gattcctgtg ccctgctatt tgttagtttg cgtaattttt ctttcatttt ttttgagtaa
     2221 ggtatatcaa ttttgtaagt aattaatgtg gcaatggcaa ctataattgg caaatcaata
     2281 aggcagcagg ttacccagaa atttatatgc atttgattgt gaaaacttgg aataataaaa
     2341 cctaaagaaa tatattgatg tatcaagtat aacgcaaatg atattttacc aaaaaaaaga
     2401 gttgttctgt ttacaataaa accaagtttg cgattcacaa ataatacaaa taagagaaaa
     2461 taaactgtca gtatgatgct atacgaatat tggctcagaa ttaccgatga gcgaccaaca
     2521 taggggaaca gaagtaattg acaaatcata caaaataata ttagtatata atttttttta
     2581 gtgtttccac tatctgtata aagattgtaa aaaatggttc cagcaaaaaa taaaggtaaa
     2641 aattgaagta agggaatcca ttttaaaagc aaacgcaccc actctgatgt ccagaaaaaa
     2701 cgggttaata tgactgttgt tacacatgta attacataaa ttatatttaa atattttagt
     2761 aatttgaatt gaaatagtaa taaaatagaa atgtaaaata ccatttcaat tatcattgtc
     2821 caatatggtc catcaaggtc agggatgttt aggtaatatt ggaacatggt taaattcccc
     2881 atgtaaataa ttatatcttt atatgaaaat gtatgcttat atatagcata tactgtaaat
     2941 aaaaaaaaag taaaggatac agatgcccaa taagtaggat ataatctgct aacccggtta
     3001 ataacaaaat caatactttt attaatgtgt tgcagactca taaaaataac aaaaccactg
     3061 attatgaaaa ataaatcaac tccggtaatt ccaattttaa aataccgatc ataaccattt
     3121 ttaccgacag taaaatgaaa aaagacgact aggactgcag ctattcctcg taaagcatct
     3181 aattcttgaa accgtttttt ttcattatta gaatttttat tttcaagcga aatgtaattc
     3241 atctactatt attgttaatt aataagttat tttagtttgc aaatgtttta agcactgtaa
     3301 taaattgaac tttttctact ttacgataat aaactattgc tgataaaaat agcatttaga
     3361 attccattta tcgtataata acgctagttt tttcatgaaa aatgtaatgc tctgttcatt
     3421 taatattcgt ttattagaag gatgcttatt aaaacaactt caacagcttt ttaaaattac
     3481 tagaaagcga agtatagtat aacattgaat ttctaagtat tgctttgatt gattattagc
     3541 taacatatag agttgttatc gttaaaggga ttagaaatgt tttctttgta atgattatgt
     3601 tttttatgct acaattatta gtatatatac tagtattcgt tttggtgtat tatttataaa
     3661 aagattaata gtctgctgat taagttcatt gaaatttatc tatcacatga tagtaaaaaa
     3721 aaaatgcgcc ttaaaagacg cattttttgt tgtttaggat tacttattta tttacccggt
     3781 cttaagctgc gtactatttt tccggtttgc cacatttcgc tttcgcgcaa ttgacgcaat
     3841 tcttcttcta atttttcacg atagtcgggt tgtgagttgg tatcgatgct gcgctgtgct
     3901 tcttcacccg cggctacttt actatataat tctttaaaga caggcaaaac cgcttctctg
     3961 aatggacctt tccagtctaa tgcaccacgt tgagctgtag tagagcaatt tccatacatc
     4021 caatccatgc cattttcaga aactagtttt ataagtgact cggttaattc ttctacggtt
     4081 tcattaaaag cctctgaagg cgagtgtccg ttatctcgta atacttgata ttgtgcttcc
     4141 atgatgccag ctaatgcgcc catcaataca ccacgctctc ccgttaaatc tgaatatact
     4201 tctttttgga aagtggtttc aaataaataa cccgaaccga taccgatgcc taatgcaata
     4261 gtacggtcaa atgcccttct tgttgcatct tgaaaaatag cataggaagc atttaagcct
     4321 gtaccttgca agaacaagcg acgtaacgat gtacctgaac cttttggagc ggtgaggata
     4381 acatctacat ccgcaggcgg cactatgcca gtacgttctt tgtaggtaat accgaaacca
     4441 tgtgaaaaat aaagtgcttt tccggcggtt aaatgttttt tcactaatgg ccacatttca
     4501 atttgagcag catcagataa aagatacata atgattgtac ctttttctag ggcttcttca
     4561 ggttcaaaaa gtgtttgacc ggctacaaaa ccgtctttct ctgctttatc ccatgatttt
     4621 gacggacgtc tttgtcctac aattacattg aagccgttat cacgcaagtt cagagcctgc
     4681 cccggacctt gaacgccata accgattaca gcgattactt catcttttaa tacttctttt
     4741 gctttggata atggaaattc ttcgcgtgtt accacgtttt cttccacacc tccgaaattc
     4801 atttttgcca tattgattta tttattagtt gtagtaatta gtgataagta gagagtagta
     4861 agtaataagt gattcgcaaa gttattcata aattccaaca ttaaatacta aacatttatc
     4921 attcaattta atcattttcc atttccagta gcgtgctttc atatacatct atttgtcttt
     4981 gcacctgttt ttttaaattt tctgcttgcc attcatcagc aatgaattcg atcgtgaatt
     5041 ttccttcacc atcatttatt ttagtaaaag aaaaatcagt aacatcaatt cttcttctgg
     5101 aaaaaataag cataaatctg cctattaata cgggttgatt gagtgaaata aattctattt
     5161 taaatttttt catggcaaag cgttgcgtaa atataaaaaa gtatttggag cagaaaatga
     5221 gattacgttt aaatctttgt caacaaagca cattaacttg taattatcca tgcatttatt
     5281 agtatgtcag tacattttat aacaaaattg gtttgcaaaa tgtaacaggc tatacttttt
     5341 ttccagacaa tcctttgttg cacgtatatg tattaaaata taaactagtc aaatgcatta
     5401 tttttagcat gaagtatcca gtagtgtttt aaatcaagta tgcgaatatc cgttgatatg
     5461 ccttccaatt tattaattaa aaaagagtgg tcggggaagt ttttcagcac ttcaaattgc
     5521 tttccatttt ccagttttct ggtttgaaaa gtattcccgt ataaatcaat tctggaaata
     5581 ttttccatcg catgcacgcc accttcaact tgtttactgt ctatgaatac aagatctcct
     5641 tcttttgtaa tcagcttact catattcaga ataaaactat ctaagtcctg aatgagtata
     5701 tgactccaaa taaaaccggc aaaaagtcca tcatatttat gtgcggtatc tattttatac
     5761 atatctgcag cttcaaactg catcggacta aaataagttc tttttttagc aatatcgatg
     5821 agtgagtgat tgatatcagt agaaaaaatg gaagaagcgg ttttcgaaat ttgttcagtc
     5881 cagtagcctg ttccacaggc tatttctatg atagattttt cagtaaaagt attttgaaaa
     5941 tagtgagcag ccgttaaaag atcttcctgt tcatctttaa tttgatatac tttttcatat
     6001 tcattagctc tttcatgata atatgttgcc agatgctcat gcattttatt tcatgttaat
     6061 acttatcgaa ttttttttgt cacctgttta ttaccttatc tgtgcgctaa gctcagtctg
     6121 ataggcagta atgagttttg ccataacttg ttcgatttcg gtatcttgca gtgttttttg
     6181 tgtatcttta aatgtgaaac ttacggcata cgattttttg tctttaccga ttttttcatc
     6241 ttcatagata tcaaaaagct caatatccgt cagtaatttt tttccggttt tttcggcaat
     6301 atttttcact tgtgcaaatg aaactgatct gtctaacaac agtgctaaat cacggcgtac
     6361 agcagggaat tttgaaatct ctttatattg tacttcttgt ttttttgtga tggttaatac
     6421 ttcatcccaa aatacatccg cataaaaaac atcattttta atagcacata atttgtttaa
     6481 agaagcattt acttttccaa aaattgctat cgtttttccg ttacataaaa tagtagatgc
     6541 atattcaaag atttcatttt ctgcattttc tgtttcaaat tttagaacac caatagaagc
     6601 taacaagctt tctaacaaag ctttgacata ataaaaatca gctttctttt cacttgtatt
     6661 ccaattcggt gatactactg ttccacttag aaatagtgcc aaatgatttt cttcttgata
     6721 agctgcatta cctgtttttg tataggtttt cccaaattca aacaactgaa tcaacgggtt
     6781 tttatgattg atattatgtg ccacactttc cagtccgctg aacaacatat tattg
//
LOCUS       DAOQII010000126         6789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6238_length_6789_cov_4.329522, whole genome shotgun sequence.
ACCESSION   DAOQII010000126 DAOQII010000000
VERSION     DAOQII010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6238_length_6789_cov_4.329522"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLF48_07180"
     CDS             complement(<1..1115)
                     /locus_tag="PLF48_07180"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF49143.1"
                     /translation="MKKQIILLATYLLSLHYIFGACGNVFLPELQQGVSTQDIRDRFQ
                     YITTFANRNSSILSSDTIRIPVVFNILYKNAVSDSLLFDKSRYYAILDTANKYLNIDI
                     NYLYTINHPSFHNIIAVPYIQLIPARLDPQGKPTDGFRFKQWTPVSGNDICGLLYFNA
                     QQKIKLDQYGGITAWDNRNYLNYWVGDYHTPVQCFSGQSTYPIFPFLTGNPLNFMDGI
                     ILQDYLIKNQNYWRNYSYSTIMHELGHYLGLLHIWGGLFDDSEKGCNIDDNIRDTPTQ
                     YQAIYHCNDSVNSCNDGANDKIDMCSNIMDYADGITYTQQQVNVMRNTLANARKMLPI
                     PKLYAQLQQADFAVCAGDSIQMVWNDLSTIQSDFVIPS"
     gene            complement(1177..2241)
                     /locus_tag="PLF48_07185"
     CDS             complement(1177..2241)
                     /locus_tag="PLF48_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF49144.1"
                     /translation="MKHTIILISICLFTFSAFAGDGQGSRFNRYKDKDREGSGHQIGI
                     SAGPMWPLTDLGGAKKIGAPFIRDIDFPSTRFAVTAFYRYNINRWVSVRANLMYGMLK
                     ADDADTDGNPPGSPGGVTDSWYRRRRNLNFTTHIVEFQAMAELNLKKYLPDVAGKGEK
                     ERWAPYVGAGLGFFWFNPWTKVGGSKVKLKPLGTEGQNLGGVYGKPYKNFQFDAMALI
                     GFKFNVTERFALAIEGVYHQTFTDYLDDVSKNYVNPDDIALLSPLAQTLQNRANDATT
                     SPYASTSFNFVEGQYKTDGSLNTGGVGQQRGDRKDNDQFMHIQLTFTVRLGDIGGKKG
                     AFGNCGRRNPYNHKFSCPKW"
     gene            complement(2287..2925)
                     /gene="nth"
                     /locus_tag="PLF48_07190"
     CDS             complement(2287..2925)
                     /gene="nth"
                     /locus_tag="PLF48_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNF49145.1"
                     /translation="MTKKEKIVFIEDTLNELYPNPPIPLDHKDAYTLLIAVLLSAQCT
                     DERVNKVTPTLFKLADNPKAMEKIPVETIKEIIRPCGLSPRKSQAISDLSKIIMHQHH
                     GKVPESFDDLEALPGVGHKTASVVMSQAFGHATFPVDTHIHRLAKRWGLSNGKSVEQT
                     EKDLKRLFAKEKWNTLHLQIIYYGREMCTARGHDEKKCIICSKLQAKNRAVK"
     gene            3052..5715
                     /locus_tag="PLF48_07195"
     CDS             3052..5715
                     /locus_tag="PLF48_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNF49146.1"
                     /translation="MSEEVVKEKVKRQKKTAVEQPPQEEAPVPLITADSIKTAAGLFL
                     LFLSIFLFVAFVSYLFTWKADQDKVLSFSWKIIFSKEALVENTMGRMGAFLAHTFFYV
                     LFGVASFFIPYILFCWGVNVTFNEKIFRVKKIILTSVIWIVFIATTAAFLFPNFAFPF
                     GGGMGTLLYDWLSTFIGRVGLGILLAILGFTFLGFNIKDTFMMLKKWKQQWEIKRATL
                     KAEKEKQKKEKQQLIAQDEFYADEEEELNKEAGYEMESILPETKEDVTPHFEDKTFEI
                     LNENTHQINPDLLEMELIEKEESVDVKESEQENELQTENDTIVELDSLPVEEKKSSRK
                     KVKEDIDFEIEELQAFGSIDTQSLLETDTNEQVPETENDSSKNDMQHFGLDVEYDPKL
                     ELSGYTLPPINLMEEHGSGQIEINKEELEQHKNQIVQTLSHYNIGIEKIKATVGPTVT
                     LYEIVPEKGIRISKIKNLEDDIALSLAALGIRIIAPIPGRGTIGIEVPNVKKSIVSMR
                     SLISGEKFQRTDFDLPVALGKTISNEDFVVDLAKMPHLLMAGATGQGKSVGLNAILVS
                     LLYKKHPAELKFVLVDPKKVELTLYKLIENHYLAQLPDEEEAIITDTKKVIYTLNALC
                     IEMDKRYDLLKSAHVRHIKEYNQKFLNRQLNPHNGHRFLPYIVLVVDEFADLMMTAGK
                     EIEGPIARLAQLARAVGIHLVIATQRPSVNIITGTIKANFPGRIAFKVTAKVDSRTIL
                     DAGGADQLIGRGDMLLSQSGEITRLQCPFVDTPEVEKIAKFIGDQRGFPTPYELPEYV
                     DPKEAGNEKQFDFGDKDDLFEEAARLIVRNQIGSTSLIQRKLALGYARSGRLMDQLEA
                     VGIVGPNLGSKAREVYVKTDEELERILQEYA"
     gene            5879..6493
                     /locus_tag="PLF48_07200"
     CDS             5879..6493
                     /locus_tag="PLF48_07200"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNF49147.1"
                     /translation="MKKQPDTKNISDPKAKILLDEVSKKYKSFSSIDAKFTFQTENGQ
                     QNKTTTENGRVYLKGEKYRVEIGKQIVICDNKSVWTYMKDINELQINDYEPNKDEITP
                     SNMFTIYQNDFDYLMNADEAIDKVNCAVVDLKPKKSDKPFFKVRIWIDKNAKYIKRMK
                     IFDKNGNRYVYTVTSFNGKANLDDAIFKFDTSKYPGVKVEDLRM"
BASE COUNT         2222 a         1201 c         1244 g         2122 t
ORIGIN      
        1 gatggaatta caaaatcaga ttgaatagtc gataaatcat tccaaaccat ctgaatactg
       61 tcgccggcac aaacagcaaa atctgcttgt tgcaattgtg cataaagttt aggtattggc
      121 aacattttcc gggcattagc cagcgtattc ctcatcacgt ttacttgttg ctgcgtgtaa
      181 gtgatgccat ccgcataatc catgatattg ctgcacatgt caattttatc attggctcca
      241 tcattgcaac tgtttacact gtcattgcaa tggtagattg cctgatactg tgttggagta
      301 tctctgatat tatcatcaat attacaacct ttttcagagt cgtcgaacaa accgccccaa
      361 atatgtaata agcccagata atgccccaat tcgtgcataa tggtcgaata tgaataattt
      421 ctccagtagt tctgattttt tatcaagtag tcctgaagta ttataccatc catgaagttt
      481 aatggatttc cggtaagaaa cggaaaaatc ggataagttg attgtccgct aaaacactgt
      541 acaggagtgt ggtaatcgcc aacccagtag tttaagtaat tgcggttatc ccaggccgta
      601 atgccaccat attgatccag ttttattttc tgttgtgcat taaaatagag taaaccacaa
      661 atatcattac cgcttacggg tgtccattgt ttaaatctaa aaccatccgt aggtttacct
      721 tgaggatcta gcctggctgg aataagttga atatagggaa cagctattat attatgaaaa
      781 ctgggatgat tgatagtata caaatagtta atatctatat tgagatattt attagccgta
      841 tcaagaattg cataatatct gcttttgtca aaaagtaaag aatcggaaac tgcattttta
      901 tacaaaatat taaagactac cggaatgcga atggtatctg aagataggat agatgaattg
      961 cggtttgcaa aagtagtaat atattgaaac ctgtcccgaa tatcttgagt agaaactcct
     1021 tgctgtaatt caggtaaaaa aacattacca caagctccaa aaatataatg aagtgatagt
     1081 aaatatgttg caagtagaat aatttgcttt ttcatagctc tataaagata aaaaaaagga
     1141 gctataaaag ctcctttttc tatgttgctt tctctgttac cacttaggac aagagaattt
     1201 atgattatat ggatttcttc ttccgcaatt gccaaatgca ccttttttac cgccaatatc
     1261 acccaagcga actgtaaaag taagttggat atgcataaac tggtcattat cttttctgtc
     1321 acctctttgt tgtcctacgc ctcctgtatt taaagaacca tctgttttat attgcccttc
     1381 aacaaaatta aatgaagtag aggcataagg acttgtagtg gcatcattag ctctgttttg
     1441 taatgtctga gcaagtggtg aaagcaaggc gatgtcatct ggatttacat aatttttact
     1501 aacatcatct aaataatcag taaaggtttg atggtaaaca ccttcgattg ctaaagcaaa
     1561 tcgttctgta acattaaact taaaaccgat taaagccatg gcatcaaatt gaaaattctt
     1621 atagggcttg ccataaacgc ctcccaggtt ttgtccttca gttcccagtg gttttaattt
     1681 tactttagaa cctccaactt ttgtccatgg attaaaccaa aagaaaccta atcctgcacc
     1741 tacataaggc gcccatcttt ctttttctcc ttttcccgct acatccggta agtatttttt
     1801 tagattcaat tctgccattg cctgaaactc aacgatatgt gtagtaaagt ttaaatttct
     1861 gcgacgtctg taccaactgt ctgttacacc tcctggagaa cccggtggat taccatcagt
     1921 atcagcatca tcagctttta gcatgccata catcagattt gctcttacag atacccatct
     1981 gttgatattg tatcggtaaa aagctgtaac agcaaatctt gttgatggga aatcaatatc
     2041 gcgaataaat ggtgctccaa tttttttagc cccgcctaaa tcagtcaatg gccacatcgg
     2101 tcctgcactg attcctattt gatgtccact gccttcgcgg tctttatctt tgtatcggtt
     2161 aaatcgcgaa ccttgtccat ctcctgcaaa agcagaaaat gtaaacaaac aaatggaaat
     2221 caggataata gtatgcttca taaaaattac tttttatctc ttaataaacg ctaaagttat
     2281 taaaaattat ttaactgcac ggtttttagc ttgaagtttt gaacaaataa tgcatttttt
     2341 ttcatcatga ccacgtgccg tacacatttc ccgaccgtaa tatattattt gcaggtgtaa
     2401 tgtattccat ttttcttttg caaataatcg ttttaagtcc ttttctgtct gttctacact
     2461 ctttccattg cttaaacccc agcgcttagc caagcggtga atatgggtat ctaccggaaa
     2521 tgtcgcatga ccaaatgcct gactcattac tacgcttgcc gttttatggc ctactcccgg
     2581 aagtgcctct aagtcatcaa aactttcggg aactttacca tggtgttggt gcataataat
     2641 ttttgataag tcagaaatag cctgagattt ccggggagat aaaccacatg gtcttattat
     2701 ttctttaatt gtttcaaccg gaattttttc catagcttta gggttatcag caagcttaaa
     2761 caaggtagga gtaactttat ttacacgttc atccgtgcat tgtgccgata acaatacggc
     2821 aattaataaa gtataggcat ctttatgatc taaaggtata ggcggattgg gatacagctc
     2881 attaagcgta tcttcaataa atactatttt ctcctttttt gtcatcagct aaatgcagat
     2941 ttgaattaaa atgtgaagct attttattac acataaacgc tttaaattat ttgcccacaa
     3001 gtgtttatta ttcttacttg ttaccggcaa ctatcctttt atatttgtaa tatgtctgaa
     3061 gaagtggtaa aagaaaaagt taagcgacaa aagaaaacag ccgttgaaca gcctccccaa
     3121 gaggaagccc ctgtgccatt aattacggca gattctatca aaaccgcagc cggtttgttt
     3181 ttattatttt taagcatttt cttatttgta gcttttgtgt cctatttgtt cacatggaaa
     3241 gccgatcagg ataaagtctt aagcttttca tggaaaatta ttttcagcaa agaagcatta
     3301 gtggagaata cgatgggcag aatgggcgca tttttagcac atactttttt ttatgtgcta
     3361 tttggtgttg cttctttttt cataccttat attttgtttt gttggggagt aaatgtaaca
     3421 tttaacgaaa agatttttcg agttaagaaa attattctca cgtcagttat ttggatagtt
     3481 tttattgcaa caacagctgc ttttctgttt ccaaattttg cttttccgtt tggtggaggt
     3541 atgggtacac tcttgtatga ttggctgtca acatttattg gtcgtgtggg cttaggcatt
     3601 ttattagcca ttttaggatt taccttttta ggctttaata tcaaagatac tttcatgatg
     3661 ttgaaaaagt ggaaacaaca atgggaaatt aaaagagcga cattaaaagc agaaaaggaa
     3721 aaacagaaaa aagagaaaca acaactgata gctcaagacg agttttatgc agatgaagaa
     3781 gaggaattga ataaagaagc aggatatgaa atggaaagca tattgcctga aacgaaagaa
     3841 gacgtaacac cgcactttga agataaaaca tttgaaattc ttaatgaaaa cacgcatcag
     3901 attaatccgg acttgttgga aatggaattg attgaaaaag aagaaagtgt tgatgtaaag
     3961 gaaagtgaac aagaaaatga attacaaacg gaaaatgata caattgtaga attggattca
     4021 ttaccggtgg aagagaaaaa atcttcacgc aaaaaagtga aagaagatat tgattttgaa
     4081 atcgaagaat tacaagcttt tggctcaata gatacacaat ctttattaga aacagataca
     4141 aatgaacaag taccggaaac ggaaaatgat tcttctaaaa atgatatgca acattttggt
     4201 cttgatgttg aatatgatcc taaattagaa ttatcaggct atacacttcc gcccatcaac
     4261 ttaatggaag agcatggtag cggacaaatt gaaatcaaca aagaagaact ggaacaacat
     4321 aaaaaccaaa ttgttcagac tttatctcac tataatatcg gtattgaaaa aataaaagca
     4381 acagttggtc ctacggttac tttatatgaa attgtacccg aaaaaggtat tcgtatatct
     4441 aaaattaaaa acctcgaaga tgatatagct ttaagtcttg cggctctcgg cattcgtatc
     4501 attgctccaa ttccaggcag aggaaccata ggcatagaag ttcccaacgt gaaaaaaagt
     4561 attgtatcca tgcgttcgct tatttcggga gaaaaattcc aacgcaccga ttttgatttg
     4621 cctgttgcct taggtaaaac catatcaaac gaagattttg tggtcgactt ggcaaaaatg
     4681 ccacacttgc tgatggctgg tgctacaggt caaggtaagt ctgtaggctt aaatgcaatt
     4741 cttgtttcac ttttgtacaa aaaacatccg gcagaattaa aattcgtgtt agtcgatcct
     4801 aaaaaagtag agcttacttt atataaactg attgaaaatc actatttggc gcaattgccc
     4861 gatgaagaag aagctattat taccgatacg aaaaaagtaa tttatacatt aaatgcactt
     4921 tgcattgaaa tggacaaacg atatgatttg ctcaagtcag cccatgtgcg ccatatcaaa
     4981 gaatataatc agaaattttt aaaccgacaa ctcaatccgc acaatggcca tcgcttcctt
     5041 ccctatatcg tattagtagt ggatgagttt gcagatttaa tgatgacggc aggaaaagaa
     5101 atagaaggcc ccattgcccg tttggctcag cttgccagag cggtaggcat ccatttagtt
     5161 attgcaacac aaagaccatc cgtcaatatt attaccggta ctattaaagc gaacttcccc
     5221 ggacgtattg cttttaaagt aacagctaaa gtagactccc gaaccatttt agatgccgga
     5281 ggagctgacc aactcattgg gagaggagat atgcttttat cgcaaagtgg tgaaataacc
     5341 cgacttcaat gcccgtttgt ggatacacct gaggtggaaa aaattgctaa atttattggt
     5401 gatcagcgtg gttttcccac accttatgaa ttacctgaat atgttgatcc caaggaagcc
     5461 ggcaatgaaa aacaatttga ttttggcgat aaagacgatt tgtttgaaga ggcagcacgg
     5521 cttatcgttc gcaaccaaat tggctctact tcgttgattc agcgtaagtt agcccttgga
     5581 tacgcccgtt caggtaggct gatggaccaa ttggaagccg ttggcatagt gggtcccaac
     5641 ttaggtagta aggctcgtga agtttatgtg aaaacagatg aagaattgga acggattttg
     5701 caggagtatg cttaattaag taacttagca aaattgataa caatttgagt gcaagattgt
     5761 aattagaaaa gtaaaactat tcactttttg aaatggctat ctggtattca acacaaaacc
     5821 tgaccgtata atgcaatatc ttctgttctc tatcttgagt ttgttctcct ttctatttgt
     5881 gaaaaagcaa ccggatacta aaaatatcag cgatcctaag gctaaaatat tattggatga
     5941 agtaagtaaa aaatataaaa gtttttcttc tatagatgcc aaatttacct ttcagacgga
     6001 gaacgggcag caaaataaaa caactacaga aaatggcaga gtatatctga aaggagaaaa
     6061 ataccgtgtg gaaattggga aacaaattgt gatttgtgac aataaaagtg tgtggacata
     6121 catgaaagat atcaacgaat tgcaaatcaa cgattatgag cctaacaaag acgaaattac
     6181 accgtctaac atgttcacca tttaccaaaa tgattttgat tatctgatga atgccgatga
     6241 agctattgat aaagtaaatt gtgccgtagt cgatttaaaa ccaaaaaaat cagataaacc
     6301 gttttttaaa gtgcgcattt ggatagacaa gaatgccaaa tacatcaagc gcatgaaaat
     6361 atttgacaaa aacggtaacc gttatgtcta taccgttaca tctttcaatg gcaaagcaaa
     6421 tttagatgat gccatcttta aatttgatac atctaaatat ccaggagtaa aagtggaaga
     6481 tttgcggatg taattatttt caaacctagt attagctatt gcgacattat aaattgatca
     6541 aaaagtttaa ttctgtcaat agtttaaaac ttatgcacac cgcctgataa ctcggttttt
     6601 tcgttttaac tgttttttgc atttttatct tggaataata tttgcattgt tccgttaata
     6661 ttatttttaa aaccgcagta tgttcaaaaa aataaccgca tttgctctgc tattttccgc
     6721 aatttttagc ttgtttgccc aaactactta tcgccagctc gatatcctga aaacgtataa
     6781 agaagcaga
//
LOCUS       DAOQII010000127         6775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6253_length_6775_cov_4.543899, whole genome shotgun sequence.
ACCESSION   DAOQII010000127 DAOQII010000000
VERSION     DAOQII010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_6775_cov_4.543899"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLF48_07205"
     CDS             <1..465
                     /locus_tag="PLF48_07205"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49148.1"
                     /translation="YFGIEWSADGIHFQPIGIVDAKGNAQNPSNYYFYHLQPQNGINY
                     YRLKMVSADSTFNYSNVLQVNLQHSPNASPKKDIVYVYPNPATNGLFMLDYYTLENKN
                     VDIKIYNTLGALVYSNQAFMQNGITSVPIDLSNLPSGLYLLKANDISYKMEK"
     gene            complement(467..2773)
                     /gene="pbpC"
                     /locus_tag="PLF48_07210"
     CDS             complement(467..2773)
                     /gene="pbpC"
                     /locus_tag="PLF48_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNF49149.1"
                     /translation="MLATLLLKNQNKLFYIFTIILLFAFYDICFPVKTQREYSTIIEA
                     KDGTVLSTFLTNDDKWRLYTKLDEITPTLQKAFIEKEDKYFYWHFGINPVAVIRAFVN
                     NTIKHRRTSGASTITMQVARMLQPKERTVFSKIREMLCALQLEWNFSKKEILQLYLNL
                     VPYGSNVEGVKSASFIYFGKAPSQLSLAEVTVLTIIPNRPTSLRLGKNNAYITEERNK
                     WLKRFKKDKVFKDEIINDALEEPLLAYRRDAPKFAPHFSLRLKRANPNIPILATNINY
                     NMQLETEELVGNYARILQNKSINNCAVMIVDNQQHELVAYIGSPDFYNSSIKGQVDGV
                     RAIRSPGSTLKPLIYGISFDKGIYTPRSVITDVPINFDGYSPENYDQHFHGKIALSDA
                     LSQSFNVPAVKVLHDIGVNTMVNYLDALDFKQIQKDKSKLGLSLALGGCGVSLEEMTN
                     LYSSFANNGKYTPPKMLQKQRDKNPIDILSPEANYMVAQILTQLRRSDLPNNAENVVN
                     LPKIAWKTGTSYGRRDAWSIGFNNQYTVGVWCGNFDGTGVPDLNGADIATPLLLQIFN
                     TISKNTAQDWFKRPKGLQFRWVCAETGNLPEHYCKNKLMALYIPGKSPNKKCEHLKKV
                     WVNADSTMSYCSACLPPDNYVEKYYPNYPPELLQYYENSNLPFQKIPKHNLLCSRIQQ
                     SQEPKIYSPTNGMVYYLNKQEKQQMQLSCQAAIDVEKISWYVNNQFIQTTVKSTPVFI
                     HPPLGKVKISCTDDKGRNTDIWVEVKEM"
     gene            2920..3159
                     /locus_tag="PLF48_07215"
     CDS             2920..3159
                     /locus_tag="PLF48_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49150.1"
                     /translation="MKWLSIKRIFLICLLWLITFSTEAQCAMCKVTAENSEYAKSLNK
                     GILLLLFFPFITIALLGIYWLKNKKKFVSDNAATE"
     gene            3172..4413
                     /locus_tag="PLF48_07220"
     CDS             3172..4413
                     /locus_tag="PLF48_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNF49151.1"
                     /translation="MTYLVQPPPTLSVHGSIQLNGSKSISNRVLLINALCNNQIDIQN
                     LSNADDTVVMQQLLQSNTKILDAGAGGTTFRFLTAYFATQKGREIILTGSERMQQRPI
                     KILVDALRLLGADIEYVGQEHFPPLRIKGRQLKGGKIELPASTSSQYITALLLIAPVL
                     EEGIQLKLTGTIVSLPYIKMTLKLMEYFGIETKMDENVITVFSGNYQPRPYFVEADWS
                     AASYFYEIAILSQEAEIELRGLTNQQIQGDAVIAKIGEDFGIHTQFNENSIRLIKTKT
                     KLPDEMSINFLEFPDLAQTVLTFSAAIGTTIDCSGLQTLRIKETDRIAALNTELSALK
                     IGSLSESNENKWVLKAFEKPPHHPQHPVFTYEDHRMAMAFAPLCLKTGSIKIEHPEVV
                     SKSYPKYWDDLVSLGFVIKEI"
     gene            complement(4447..4992)
                     /gene="infC"
                     /locus_tag="PLF48_07225"
     CDS             complement(4447..4992)
                     /gene="infC"
                     /locus_tag="PLF48_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNF49152.1"
                     /translation="MIPRRKVFVPRKKETEHRLNKDIRVPEVRLVGENVEVGVYPTER
                     ALEMAKEQELDLVEISPNAVPPVCRIVDYNKFLYDKKKKEKEIKAKQKKTEVKEIRFT
                     PNTDDHDLDFKKKHAIKFLQEGSKVKIYVQFRGRAIQFKDRGELVLLQFADALKEYGA
                     LEAMPNLEGKKMTAFIAPKKK"
     gene            complement(5039..5422)
                     /locus_tag="PLF48_07230"
     CDS             complement(5039..5422)
                     /locus_tag="PLF48_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF49153.1"
                     /translation="MEDKIHSFESLECWKDAKEIQIAIYNLVKVFPENERYRLTDQMI
                     RCSRSITNNIAEGYGRFHYLDNAKFCRNSRGSLMELLDHLIIANDCGYINDEKLESLR
                     KRINKCNALLNGYIRYLLKAKNELE"
     gene            complement(5471..>6775)
                     /gene="thrS"
                     /locus_tag="PLF48_07235"
     CDS             complement(5471..>6775)
                     /gene="thrS"
                     /locus_tag="PLF48_07235"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNF49154.1"
                     /translation="EKRKQLTRIYGITFPNQQELDEYLKMLEEAEKRDHRKIGQQLEL
                     FAFSQKVGSGLALWLPNGTFIREQLAEFLKKEQIKRGYKPVITPHIGKKDLYVTSGHY
                     EKYGEDSFKPILTPEENEEFYLKPMNCPHHCEIFKHIPKSYKDLPYRVAEYGTVYRYE
                     QSGELHGLTRVRGFTQDDAHLFCTPEQVKDEFKSVIELVQLVFKKLGFEHFTAQISLR
                     DPENKEKYIGSDENWEKAESAIVEAANEMGLTTVTEYGEAAFYGPKLDFMVKDVIGRS
                     WQLGTIQVDYNLPERFELEYVGADNEKHRPVMIHRAPFGSFERFIAILTEHCAGNFPL
                     WLAPKQISILPISDKFAEYAEQISKALQQKEFRTNVDLRSEKIGKKIRDAEMMKIPYM
                     LVIGEKEVENKQISVRKHGKGDLGTFTIEDFIVHLQNELNSQ"
BASE COUNT         2023 a         1321 c         1144 g         2287 t
ORIGIN      
        1 tattttggca tagagtggtc ggccgatggt atccattttc agccgattgg cattgttgat
       61 gcaaaaggca atgctcaaaa cccgagcaat tattattttt atcatctaca gcctcaaaac
      121 ggtattaact attaccggct taagatggtt tctgcagact ctacctttaa ttattccaac
      181 gttttgcagg tcaatttaca acattcacca aatgcttcgc caaagaaaga tatagtatat
      241 gtctatccca atccggctac aaatggcttg tttatgttgg attattatac acttgaaaat
      301 aaaaatgttg acatcaaaat ttataataca ctcggagctt tagtttacag caatcaggca
      361 tttatgcaaa atggcattac atctgttccg atagacttaa gtaatctacc atcgggattg
      421 tatcttttaa aagcaaatga catttcttat aaaatggaaa aatagactac atctctttta
      481 cttctaccca aatatctgta tttcttcctt tatcatctgt acacgaaatt tttaccttgc
      541 ccaatggcgg atgtataaaa acaggtgtgc ttttaactgt tgtctgaata aactgattat
      601 tgacatacca cgatattttc tcgacatcta ttgcagcctg acaactcaac tgcatctgct
      661 gtttttcttg cttattgaga taatatacca tgccgttagt aggactgtaa attttgggtt
      721 cctgactttg ttgaatacgt gagcaaagta gattgtgttt aggtattttt tggaatggta
      781 aattgctgtt ttcatagtat tgcaataatt caggcggata attggggtaa tatttttcga
      841 cataattatc cggaggtaaa cacgcactgc aatagctcat ggtactatca gcatttaccc
      901 aaactttttt caaatgttcg cattttttat tcgggctttt cccgggaata tataatgcca
      961 tgagcttatt cttacaataa tgttcaggca aatttccagt ctccgcacaa acccacctga
     1021 attgcagtcc tttcggacgc ttgaaccaat cttgcgcagt attcttactg atggtattaa
     1081 agatttgcaa taacaatggc gttgcaatat ctgctccatt taaatcaggc acacccgtgc
     1141 cgtcaaaatt gccacaccac actcctactg tatattgatt attgaaacct atactccagg
     1201 catctcttct gccataagat gttccggtct tccatgctat ctttggcaag ttcactacat
     1261 tttctgcatt gttcggcaaa tcacttcttc ttaattgtgt gagtatttgt gcaaccatat
     1321 aatttgcttc gggagaaagg atgtcaatgg gatttttatc ccgttgtttt tgcagcattt
     1381 tgggtggagt atattttccg ttattggcaa atgagctgta caaatttgtc atttcttcca
     1441 agctcacccc acagccacct aatgccaaac tcaatcctaa ttttgattta tctttttgta
     1501 tttgcttaaa atccagtgca tctaaataat tgaccatcgt atttacgccg atatcgtgta
     1561 acactttcac ggccggcaca ttaaacgact gcgacaaggc atcactcaag gcaattttgc
     1621 catgaaaatg ctggtcataa ttttccggac tgtacccgtc aaaatttatg ggaacatctg
     1681 taataactga gcgaggcgtg taaattcctt tatcgaaaga aattccataa atgagtggct
     1741 ttaacgtaga acccggtgaa cgaatagctc gaacgccatc cacctgacct ttaatactac
     1801 tattataaaa atcgggagag ccgatataag cgacaagttc gtgttgctga ttgtcaacta
     1861 tcatcacagc acagttattg attgatttat tttgtaaaat cctggcataa ttgcctacta
     1921 actcttccgt ttcgagttgc atattataat tgatatttgt agccagtatg ggtatgttgg
     1981 gattggcacg tttcaagcgt aaagaaaaat gaggtgcgaa tttcggtgca tctctccgat
     2041 atgccaatag tggttcttca agtgcatcgt taatgatttc atccttaaaa accttatctt
     2101 ttttgaatct tttcaaccac ttattgcgtt cttccgtgat ataggcatta tttttcccca
     2161 gtcttaatga agttggtcgg ttgggaatga tagtcaatac ggtaacttct gccaggctaa
     2221 gctgcgaagg agctttaccg aaatagataa atgatgctga ttttacaccc tctacattgc
     2281 tgccatacgg cactaaattg agataaagct gcagtatttc ttttttagaa aaattccatt
     2341 ccaattgcaa ggcacacagc atttctctga ttttagaaaa cacagttctt tctttgggtt
     2401 gtagcattcg agcaacttgc atggtgatag tagaagctcc tgatgtgcgt cgatgcttta
     2461 tcgtattatt gacaaatgct cttattactg caaccgggtt tattccgaaa tgccaataaa
     2521 aatatttgtc ttctttttcg atgaatgcct tttgcaaagt gggtgtgatt tcatctaatt
     2581 ttgtatacag ccgccatttg tcatcattcg ttaaaaatgt ggaaagcaca gtgccatctt
     2641 tagcttcaat gattgtagaa tattcgcgtt gcgtttttac cgggaagcaa atatcataaa
     2701 atgcaaataa gagtattatt gtaaaaatat aaaataattt attttgattt ttcaaaagca
     2761 aagtagccaa catgaacaga aaattagaca tttaattcaa cagtgtaagt tataaaagat
     2821 taatatcagc ccgcaatatt aaactggatt tttagtatat ttaattttac tttaatacta
     2881 acatagggaa gtgtgttgat tattttttat ctttattgca tgaaatggct aagcattaag
     2941 cgcatatttt tgatttgcct attatggcta ataactttta gcacagaagc gcaatgtgct
     3001 atgtgcaaag tgactgccga aaattcggaa tatgccaaaa gcctgaacaa gggaattcta
     3061 cttttattat tttttccatt tatcaccata gcgttgcttg gcatttactg gcttaaaaac
     3121 aaaaagaagt ttgtcagtga taatgctgca accgaatagc cgactaaagt tttgacttat
     3181 cttgtacaac cacctcccac tctttccgtt catggcagca ttcagctcaa tggttccaag
     3241 agtatcagca atcgtgtatt actgattaat gctctttgta ataatcaaat tgacatacaa
     3301 aatttatcaa atgcagatga caccgtagtc atgcaacaat tactgcaaag caatacaaag
     3361 atacttgatg ctggtgcagg tggtactact ttcagatttc taactgctta ttttgctacg
     3421 caaaaaggaa gagagattat attgacaggc agcgaaagga tgcagcaacg acccataaaa
     3481 attttagtag atgccctacg cttgcttggt gccgacatcg aatacgtggg tcaggagcat
     3541 tttcctcccc tgcgtataaa aggaaggcag ttgaaaggcg gtaaaatcga attgcctgcc
     3601 agtacttcat cacaatatat aacggcttta ttgctaattg caccagtgct ggaagaagga
     3661 atacaactta aattaacggg tactatcgtt tcattgccat atattaaaat gacgttaaaa
     3721 ctcatggaat attttggtat cgaaacaaaa atggatgaaa acgtaataac tgttttttcc
     3781 ggaaactatc agcctcgacc ctattttgtt gaagcagact ggagtgcagc ttcttacttt
     3841 tatgaaattg ccatcttatc acaagaagcc gaaattgaac tgcgcggttt aaccaatcaa
     3901 caaatacagg gagatgcggt cattgcaaaa atcggcgaag attttggtat ccatactcaa
     3961 tttaatgaaa acagcatacg gttgataaaa acaaaaacta aactaccgga tgaaatgagc
     4021 ataaattttt tggaatttcc ggatttagca caaaccgttc tcacattttc tgctgcaatt
     4081 gggactacaa tagattgctc aggtttgcaa actttgcgca taaaagaaac agatagaatt
     4141 gcagcattaa atacagagtt aagcgcatta aaaataggct cactctctga aagtaatgaa
     4201 aacaaatggg tgttaaaagc atttgagaaa ccaccacatc atccacaaca ccctgttttt
     4261 acttacgaag accacaggat ggccatggct tttgctccct tgtgtttaaa aacaggaagt
     4321 ataaaaatag aacatccgga agtagtcagt aaatcatatc ccaaatactg ggatgattta
     4381 gtatccttag gttttgtgat aaaagaaata tgaattaatc agctttaatg aattataata
     4441 cagcctttat tttttcttag gggcaataaa agcagtcatc tttttacctt caagattagg
     4501 catagcttcc aaagcaccgt attctttaag tgcatctgca aactgcagga gtactaattc
     4561 ccctcggtct ttgaattgta tagcacgacc acggaattgc acatagattt tcactttaga
     4621 gccttcctgc aaaaacttga tggcatgttt tttcttaaaa tccaaatcat gatcatcggt
     4681 gttcggagtg aaacgaattt ctttcacttc cgtttttttc tgcttggctt taatctcttt
     4741 ttcctttttc tttttgtcgt aaaggaattt gttgtagtca acaatacgac aaaccggcgg
     4801 tactgcattc ggtgagattt ccaccaaatc caattcctgt tctttagcca tttccaatgc
     4861 acgctcagta gggtatacac ctacttctac gttttctccc acaagacgaa cttcaggtac
     4921 acggatgtct ttgtttaatc ggtgttccgt ttctttttta cgcggaacga atacttttct
     4981 tctaggtatc aagttttttt aatttatgtt ataggttatt tgtaacaagt tatctggttt
     5041 attctaattc attttttgcc tttaacaaat atctgatata tccatttaac agtgcattac
     5101 atttatttat tcttttcctc aaactctcca acttttcatc attaatgtaa ccacaatcat
     5161 ttgcaattat caagtgatct aacaattcca ttaaagaccc tctgctattt ctacaaaatt
     5221 ttgcattatc taaataatga aatcgcccat acccttctgc aatgttattt gttatacttc
     5281 tggaacatct tatcatttgg tctgtaagtc tgtatcgctc attttccgga aataccttaa
     5341 ctaaattata aatagctatc tggatttctt tagcatcctt ccaacattcc aaactttcaa
     5401 atgaatgaat tttatcttcc aaatcagata ccgaataatt tacaaataac ttattacata
     5461 taacaaataa ctattggcta tttagctcat tttgtaaatg tacgatgaaa tcttcaattg
     5521 taaaagtgcc taagtcgcct ttgccgtgtt ttcttacgga tatttgctta ttttctactt
     5581 ctttttctcc tataacgagc atatacggta ttttcatcat ctcggcatcg cgaatttttt
     5641 taccgatttt ctcgctgcgc aagtccacat tggttctgaa ttctttttgt tgtaatgctt
     5701 tgcttatttg ttcggcatac tcggcaaatt tatccgaaat aggtaaaata gaaatttgct
     5761 taggtgctaa ccataatggg aaattgcctg cacaatgctc tgttaaaatt gctataaaac
     5821 gttcgaaaga gccaaatggc gcacgatgta tcattacggg ccgatgcttc tcattgtctg
     5881 cacctacata ttccaattca aatctttccg gaagattata gtcaacttgt atggtaccta
     5941 actgccagct tctgccaatg acgtctttca ccataaaatc tagtttaggt ccataaaatg
     6001 cagcttctcc atattcagta acagtagtta atcccatctc attagcagct tctacaatag
     6061 cactttctgc tttctcccag ttttcatcag agccaatata tttttctttg ttttccggat
     6121 cacgcaatga aatctgagca gtaaaatgtt caaaacccag ctttttgaat accaattgaa
     6181 ctaactcgat cacactctta aactcgtctt ttacctgttc cggagtacaa aataaatgtg
     6241 catcatcctg tgtaaagcca cgaacacgtg ttaaaccgtg taattctcca ctttgctcat
     6301 agcgatagac tgtaccatac tcagcaacac gataaggtaa atctttataa gatttaggaa
     6361 tgtgtttaaa tatttcgcaa tgatgcggac agttcatcgg ctttaaatag aactcctcat
     6421 tttcttccgg tgtcaatatc ggtttaaaag aatcttcacc atatttttca taatgacctg
     6481 aggttacata taaatctttt tttccaatat gtggagtaat cacaggttta tatccgcgtt
     6541 taatttgttc ttttttcaaa aattctgcta attgctcacg aataaaagtt ccgtttggaa
     6601 gccataatgc taagccagat cccacctttt gagaaaaagc aaaaagttcg agttgctgtc
     6661 caatttttct gtgatcgcgt ttttctgctt cttccagcat ttttaggtat tcgtctaatt
     6721 cctgctgatt tggaaaagta ataccataaa tacgggtaag ctgtttgcgc ttttc
//
LOCUS       DAOQII010000128         6743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6293_length_6743_cov_4.747458, whole genome shotgun sequence.
ACCESSION   DAOQII010000128 DAOQII010000000
VERSION     DAOQII010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_6743_cov_4.747458"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLF48_07240"
     CDS             <1..195
                     /locus_tag="PLF48_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49155.1"
                     /translation="IKRKNLTYVEVFTSLINRNQLALATAFFDFFNLDLPKEPLKILP
                     LLLLLSPFPIIIYFFVRMQR"
     gene            415..499
                     /locus_tag="PLF48_07245"
     tRNA            415..499
                     /locus_tag="PLF48_07245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:449..451,aa:Ser,seq:tga)
     gene            554..630
                     /locus_tag="PLF48_07250"
     tRNA            554..630
                     /locus_tag="PLF48_07250"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:588..590,aa:His,seq:gtg)
     gene            complement(639..1175)
                     /locus_tag="PLF48_07255"
     CDS             complement(639..1175)
                     /locus_tag="PLF48_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNF49156.1"
                     /translation="MNKLQITSDWTLFLDRDGVINKKIENGYVLSPDDLEFLPGVFPF
                     LAEAANLFQKIIVVTNQQCIGKGLLTVEALNEINSYILNFVELNGGRIDAVYFAPDLA
                     GEYNTLRKPATGMAELAKADFPEINFEKSLMIGDSLSDMQFGKVLGMTTIFLNAEKVK
                     SDLIDFKSGSWKDILELF"
     gene            complement(1172..1861)
                     /locus_tag="PLF48_07260"
     CDS             complement(1172..1861)
                     /locus_tag="PLF48_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF49157.1"
                     /translation="MNTYPVIILAGGKGTRLSSVVSDVPKPIALVNGKPFLHYIFSYL
                     QKQQIKQVILSVGYLSDVIIQYFGSEYLGIKIKYVIEKEALGTGGAIKYAFEQIDEAA
                     YVINGDTFFNIDLKQLANKHADAVIALKPMEKFERYGTVELDNDSRIIHFLEKQYCEE
                     GLINGGIYFIQKSVFDKIQTPDSFSMEKNIFEKHADDLKIQGKIFTDYFIDIGIPEDY
                     YLAQHYFSSLL"
     gene            complement(1861..2433)
                     /locus_tag="PLF48_07265"
     CDS             complement(1861..2433)
                     /locus_tag="PLF48_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNF49158.1"
                     /translation="MQDKISKILQSSIDIKREILTNYNLLNQIEQVVALTTVCFKNGG
                     KVLTCGNGGSAADAQHLSAELSGRFYKDRNPLFAEALHVNTSYLTAVANDYSFDEVYA
                     RLVKAKGKNGDILWGFSTSGNSENILRAFIQAKASDMITIGFTGESGGKLASLCDILI
                     NVPSTDTPRIQECHIVIGHIICELIEDNLF"
     gene            2471..3124
                     /locus_tag="PLF48_07270"
     CDS             2471..3124
                     /locus_tag="PLF48_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HNF49159.1"
                     /translation="MGTIELDTATKIININADFITYGDSASYELSVQLAKETEDMWNE
                     PNGMVMLNSMPYSLHFKIGAQYNNLIAPETIINNTNPLHNFFRVEDYIAGNISHVDGI
                     GSNSGYLLIDNLYAGSTTIAHEFGHTLGLVHPANTNIVGQGVPGIMYPRGTLVNPEFQ
                     YDIHAAPGGPRGTINPIHRRVKQEDIDNLQIAEHIYHSIFIIGRFTNKYHLKQIKPN"
     gene            3360..4436
                     /locus_tag="PLF48_07275"
     CDS             3360..4436
                     /locus_tag="PLF48_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49160.1"
                     /translation="MSISAFSQTKDEIILLGKNDLLQKQYAPAIQLFTNLYKTDTKDI
                     DGIYWLSYAQTLAGNYEQADKLLKQGIQLKPDYFGFYDVYGQYFFLQNDFDNALLYFN
                     ESLKLNPANATSLNTRGMCYNYLGKYDLAFADFNSAILIDSTLAAAYNNRATARYNNQ
                     NIAKASLTDLHFALIDLNKCLMLDNNNALAYRNRGIVYFYLDSMFLAKQDLNTAVVLN
                     SADVNAHYFLGKTLYHLDEKESALTQFNKAIQLENYKAEFYMDRGIVLTDLKQFAAAR
                     TDFYQAQQISNDFKGIANYQNARSYAAEGNKKQMIVALKAANKVKLFKKYPAIFNEIK
                     QDEYFMKFEKDKDFYDLLQKFKFD"
     gene            4515..5780
                     /locus_tag="PLF48_07280"
     CDS             4515..5780
                     /locus_tag="PLF48_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF49161.1"
                     /translation="MTRAVIRNIVLFAFISLAGIIFSQLYWIKKALDLKEEQFNHRVN
                     VTLKSVADHLINDTRRNVILDGINRKSNNNYTIDFSAPVNVSKLEHYLFIESKNQNLN
                     SDFEYGIFECTTDSFYAGRLLDFKNIGSLETLDVHANTGENYYIGILFPKKKTLLEND
                     LRLLMFFTLVLLLVIGFFTYTILVILKQKRLSEIKTDFVNNMTHEFKTPISTISLASD
                     VLLRDDIAEKPEKLKHYATIIQAENNRLKSQVESVLQVSQIESHKIQLNLDVVDMHAL
                     IERIVKNMEIRVHDAGGEIVMNLNATQHFISGDEVHLTNIIYNLLDNALKYCDKIPHI
                     EITTVNKHRGLEIYVKDNGKGIDKTSQSMIFQKFFRVPSGNVHDVKGFGLGLFYVENM
                     LKAHKGKISVQSTPKVGTTFILWFKTLKK"
     gene            5816..6493
                     /locus_tag="PLF48_07285"
     CDS             5816..6493
                     /locus_tag="PLF48_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF49162.1"
                     /translation="MSKILLVEDDPNLGFIIKDNLSDKGYEVTHCINGLEAERLLFKE
                     HFDLYLFDVMMPKKDGFTLAEELRAKKDNTPIIFLTAKEMQEDKLRGFTVGGDDYITK
                     PFNFDELIMRMEAILKRTKPAEPEPTTFKIGKYTLKSDELKLILGKEEQSITKKESAL
                     LEFLAKSLNQPVKREDMLMKIWKDDSYFAGRSMDVYITKLRKYLKDDPNVELLNVHGV
                     GFKLVAR"
     gene            complement(6516..>6743)
                     /locus_tag="PLF48_07290"
     CDS             complement(6516..>6743)
                     /locus_tag="PLF48_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNF49163.1"
                     /translation="EIGKNLPNVIDTSLTKIEQKLDIHQLVKNKVEAFSDEKLENLLL
                     DITSKEFTFIEIIGGVLGLLIGIIQLLISNL"
BASE COUNT         2275 a         1171 c         1199 g         2098 t
ORIGIN      
        1 ataaaaagaa aaaacctcac ttatgttgag gtttttacat ctttgattaa tagaaatcaa
       61 ttagctttag caacagcctt ttttgatttt tttaatctgg atttaccaaa agagcctctg
      121 aaaattttac ctcttttact tctgctatca ccttttccca taataattta tttttttgta
      181 agaatgcaaa gataagcaga atatcaatat tttcagccct tctgcaaaaa aaatacgcct
      241 gttttttgaa tatttaatgt aaaagttaaa aacacgtatg gaagaatgct taaaaatgtt
      301 tatttttgtg cgggcaagtc ttatacgacc agctccctgc aaatcctcca gggtcggaag
      361 gcagcaaagg tagtttggtt gtagcggtgc gatataagtc gcttgccttt ttttagagag
      421 atggcagagc ggtttaatgc ggcggtcttg aaaaccgttg actgtaaaag gtccgggggt
      481 tcgaatccct ctctctctgc gtaaaaatct tgctctcaga gcaagatttt tagtagaagc
      541 tcttttattt atggacaaca tagctcagta ggttagagca tcggattgtg gttccgaggg
      601 tcgtgggttc gaaccccatt gttgtcccca ttttttttct agaataattc taaaatatct
      661 ttccagctcc cggatttaaa atcaatcaaa tctgatttta ctttttctgc atttaagaaa
      721 atagtagtca tgcccaatac tttaccaaac tgcatatccg aaagtgaatc gcctatcatc
      781 agactttttt caaagttgat ttcaggaaaa tcagcctttg ccaactcagc catgccggta
      841 gccggttttc tcagcgtatt atattctccg gcaagatcag gagcaaaata aacagcatca
      901 attcgtccgc cattcaattc aacaaaattt aaaatataac tgttgatttc attgagtgct
      961 tccaccgtga gcaatccttt gccaatgcat tgctgattag taaccacaat tattttttga
     1021 aacaggtttg cagcctcagc taaaaatggg aatacacccg gcaaaaattc caaatcatcg
     1081 ggacttaaga catagccgtt ttcgattttt ttattgatta caccatcacg gtctaaaaat
     1141 aaagtccagt cagatgtgat ttgcaatttg ttcatagtaa ggatgaaaag taatgttgtg
     1201 ccagataata atcttcagga atgccaatgt ctataaaata atcggtgaaa attttgcctt
     1261 gaatctttaa atcatcagca tgtttttcaa aaatattttt ttccatggaa aaactatccg
     1321 gtgtttggat tttgtcaaaa accgattttt gaataaaata tattccgccg ttaatcagcc
     1381 cttcttcaca gtattgtttt tccagaaaat gaataatcct gctgtcgttg tccaattcaa
     1441 cagttccata gcgttcaaat ttttccatgg gcttcaaggc gattacggca tctgcatgtt
     1501 tatttgcaag ttgttttaaa tcaatattaa aaaaggtgtc gccattaatc acatacgctg
     1561 cttcatcgat ctgctcaaag gcatatttta ttgcccctcc ggtacctaac gcttcttttt
     1621 ctattacata tttaatttta attcccaagt actcgctccc gaaatattgg ataatcacat
     1681 ccgacaaata gccgacagat aaaataacct gctttatttg ctgtttttgc aaataactga
     1741 atatataatg cagaaatggt ttgccattaa ctaaggctat tggtttgggt acatcactga
     1801 ctacactgct cagtcgtgtg cctttacctc cggctaaaat gataacggga taagtattca
     1861 tcagaataaa ttgtcttcaa tcaattcaca aatgatatga ccaattacga tatggcattc
     1921 ttgaattctg ggcgtatccg ttgacggtac attgatcagt atatcgcaca gcgaagcaag
     1981 tttaccgcca ctttctccgg taaaacctat agtaatcatg tcggatgctt tagcctgtat
     2041 aaatgcacgc aggatatttt cggaatttcc agatgtcgaa aatccccaga gaatatcacc
     2101 gtttttgcct tttgctttta ccaaacgtgc atacacttca tcaaaactat aatcgttggc
     2161 aacggctgtt aaataagatg tatttacatg caatgcttct gcaaacaagg gattgcggtc
     2221 tttataaaac cttccggaca gttctgcaga aagatgctgt gcatcggctg ctgaaccgcc
     2281 attcccgcaa gtcagtactt ttcctccatt cttaaaacaa actgtagtca atgccactac
     2341 ttgctcgatt tggtttaata aattatagtt tgtcagaatt tctcttttga tatcgataga
     2401 actttggagt attttactaa ttttatcttg catgatataa agataatcat ttcacattaa
     2461 ttcgatacac atgggtacta tagagctaga tactgcaaca aagataataa acataaatgc
     2521 cgattttatt acctatggtg atagtgcttc ctatgagtta agtgttcagt tggcaaaaga
     2581 aaccgaagac atgtggaatg agccgaatgg catggtgatg ttgaatagta tgccgtattc
     2641 attgcacttt aaaatcggtg ctcaatataa taatctgata gctcctgaga ccattataaa
     2701 taatacaaat ccattgcata attttttcag ggtagaagat tatatcgcag gcaatatttc
     2761 gcatgtggat ggcattggca gcaattccgg ttatttgctg attgataatt tatatgcagg
     2821 atcaacaact attgctcacg aatttggtca tactttgggc ttggtgcatc ctgcaaatac
     2881 gaatattgta ggtcagggtg ttccgggtat catgtatccc agaggtacgc tggtcaatcc
     2941 ggaatttcag tatgacatac atgctgctcc gggtggcccg agaggtacga ttaacccgat
     3001 tcatcgccgg gtaaaacaag aagatatcga caatctgcaa attgcagaac atatctacca
     3061 cagcattttt atcatcggaa gatttaccaa taaatatcat ttgaagcaga ttaaaccaaa
     3121 ctaaaataac cagaaaggaa gacaacagga gataagctat ccctgaaatc gatgaaacca
     3181 atagctgtgg tacgtttttt gaaagaaata ttgataaaag aagtcatata gcttatatta
     3241 gattgaaaat atggttgaaa agattactac tcatgcaaaa taattataac gataagtctt
     3301 taattttttt gaacaaacaa tggatatgtg ccatttcctt attttgcatt tttactttca
     3361 tgagcatttc tgctttttca caaactaaag acgaaattat tttattgggg aaaaacgatc
     3421 tactgcaaaa acaatatgcc cctgctatac aactttttac aaatttgtac aagacagata
     3481 ctaaagatat agatggcatt tactggttga gttatgcgca gacattagca ggtaattatg
     3541 agcaagcaga taaattactc aagcaaggca ttcagttgaa gcccgattat tttggatttt
     3601 acgatgtata cggacaatat ttttttttgc aaaatgactt tgataatgca cttttatact
     3661 ttaacgaatc gctgaaactg aacccggcaa atgccacatc attgaacaca cgcggcatgt
     3721 gttataatta tttgggaaag tatgatttgg catttgctga ttttaacagt gccatactaa
     3781 tagacagtac actggctgcc gcatataaca acagagccac agcaagatat aataatcaaa
     3841 atatagccaa agccagtttg acagatttac attttgccct gatagattta aacaagtgtc
     3901 taatgctgga taataacaat gcactggcat acagaaaccg aggtatcgtt tatttttatt
     3961 tagacagtat gtttttagcc aaacaagatt taaatacagc cgtcgttttg aatagtgctg
     4021 atgtcaatgc ccattatttt ttaggtaaaa cactttatca tctggatgaa aaagaatcgg
     4081 cactcacgca atttaataag gctatacagc tcgaaaatta caaggcagaa ttctatatgg
     4141 acaggggcat tgttttaact gatttaaaac agtttgcagc agctcgaaca gatttttatc
     4201 aggcacaaca aatcagcaat gattttaaag ggatagccaa ttaccaaaat gcgcgcagct
     4261 atgccgccga aggcaataaa aagcaaatga ttgtagcgtt gaaagccgca aataaagtaa
     4321 aactgtttaa aaaatatccg gccatcttta atgaaataaa acaagatgaa tatttcatga
     4381 aatttgaaaa agataaagac ttttatgatt tacttcagaa attcaaattt gattaatgct
     4441 aatcatttcg atacattttt gctaaaataa tgttaatcgt ctttgtatac caatttatta
     4501 tttagtttta caaaatgacc agagcagtca tacgcaatat tgtattattc gcctttattt
     4561 cattggcggg tattattttt tctcaactat actggattaa aaaagcactg gatttaaaag
     4621 aagaacaatt caaccaccgg gtaaatgtaa ctttaaaaag tgtagccgac catctgatta
     4681 atgatacccg acgaaatgta atattagatg gaatcaaccg caaatcaaat aataactaca
     4741 ctattgattt tagcgcacca gtaaatgttt ccaaactcga gcactattta tttattgagt
     4801 ccaaaaatca aaatctgaat tctgattttg aatacggaat atttgaatgt accaccgatt
     4861 cattttatgc cggtcgcctg ctggatttta aaaacatagg cagtctcgaa acactagatg
     4921 tacatgccaa taccggagaa aattattata tcggtatttt attcccaaaa aagaaaacac
     4981 ttctggaaaa cgacttgaga ttactcatgt tttttacttt agtattacta cttgtaatag
     5041 gattttttac atataccata ttagtcattt taaaacaaaa aagattaagc gagataaaaa
     5101 cagatttcgt gaataatatg acgcatgagt ttaaaacgcc tatttcaaca ataagtctgg
     5161 ccagtgatgt actgttgcgg gatgatattg ccgagaagcc cgaaaaactg aaacattatg
     5221 ccacaattat tcaagctgaa aataacaggt tgaaaagtca agtagagtcc gttctgcaag
     5281 tttcgcaaat tgagtcacat aaaattcaat tgaatttaga tgtagtagat atgcatgcac
     5341 tgattgaaag aattgtaaaa aatatggaaa ttcgggttca tgatgctggt ggcgaaatag
     5401 taatgaattt aaatgccacg caacatttta tatcgggaga cgaagtacat ttaactaata
     5461 ttatttataa cctgttggat aatgcactaa aatattgtga caaaattcca catatagaaa
     5521 ttacaacagt aaataaacac cgaggtctcg aaatttatgt aaaagacaat ggaaaaggta
     5581 ttgacaaaac atcacaaagc atgattttcc aaaaattctt cagagtaccc agtggcaatg
     5641 tacatgatgt aaaagggttt ggattgggct tgttttatgt ggagaatatg ttgaaagcac
     5701 ataaaggaaa aataagtgtg caaagtacac caaaagttgg caccactttt atattatggt
     5761 tcaaaacttt gaaaaaatag gataataatg ataactttaa aaataaaata aacctatgag
     5821 caaaattcta cttgtggaag atgatccgaa tcttggattt attattaaag ataatctgag
     5881 cgataagggt tatgaggtta cgcattgcat aaatggactg gaagccgaac gactgctttt
     5941 taaagagcat tttgatttgt acttgtttga tgtaatgatg cctaaaaaag atggatttac
     6001 acttgccgaa gaactgcggg caaaaaaaga caatacgcct atcatctttc tgacagcaaa
     6061 agaaatgcag gaagataaat tgcgcggatt tacagtggga ggcgatgatt atatcaccaa
     6121 gcctttcaat ttcgatgaat tgataatgcg catggaggca attttaaagc gaactaagcc
     6181 cgctgaaccg gaacctacta cattcaaaat agggaaatat acactgaagt cagatgaact
     6241 gaaacttatt ttgggcaaag aagaacaatc cattacgaag aaggaaagtg cattgttgga
     6301 attcttggca aaaagcctga atcaacccgt gaaacgggaa gatatgctga tgaaaatctg
     6361 gaaagacgac agttattttg ccggaagaag catggacgtt tatattacta aactccgtaa
     6421 atatctcaaa gacgacccga atgtagaatt gctcaacgtg cacggtgtcg gttttaaact
     6481 ggttgcaaga taaaaatctt aaagctaaat aagtgttaca gattgctaat cagcaattga
     6541 attataccaa tcaataaacc taatacacct ccaataattt ctatgaaagt gaattctttg
     6601 gaggtaatat ctaatagcag attttccagc ttttcatctg aaaatgcttc tactttgttt
     6661 tttactaact gatgaatgtc tagtttctgt tcaattttag ttaacgaagt atcaataaca
     6721 ttaggcaagt ttttgccaat ttc
//
LOCUS       DAOQII010000129         6659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6415_length_6659_cov_3.851151, whole genome shotgun sequence.
ACCESSION   DAOQII010000129 DAOQII010000000
VERSION     DAOQII010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6415_length_6659_cov_3.851151"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLF48_07295"
     CDS             <1..888
                     /locus_tag="PLF48_07295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223197.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNF49164.1"
                     /translation="YLRVIIMELSRIADHIVCDTVIAVDTGAMTGFLHLFQYREWIYE
                     IFEEICGSRLTTNIGRIGGLNADFSQQTWDKINHFMAEFPKGLKEFQKLVDRNRIFMD
                     RTINAGPITAEKALAYGFTGPNLRAAGVDYDVRVANPYSSYEDFTFEIPIGTNGDTYD
                     RYMVRMEEMWQSYSIVEQAIKNLPDGKPHADVPDFFLPPKEAVYNNMEALIYHFKIVM
                     GETDIPKGEVYHAVEGGNGELGFYLISDGGRTPFRLHFRRPCFIYYQAYPEMIKGAML
                     SDAILTMSSMNVIAGELDA"
     gene            985..1323
                     /gene="yajC"
                     /locus_tag="PLF48_07300"
     CDS             985..1323
                     /gene="yajC"
                     /locus_tag="PLF48_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNF49165.1"
                     /translation="MILAFFLQAAPAAGGNSPMSFIFMLCLMFVVFYFFLIRPQQKKQ
                     KEAQEFSNNMKIGDRIITIGGVHGKIVAINDDNTYTIQVDGPTRLKIEKSAVNAEASK
                     ALQQNTGTNA"
     gene            1337..1921
                     /gene="coaE"
                     /locus_tag="PLF48_07305"
     CDS             1337..1921
                     /gene="coaE"
                     /locus_tag="PLF48_07305"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNF49166.1"
                     /translation="MLKVGITGGIGSGKTTVCKIFELLGISVYYSDLRAKQLMTENQL
                     VKQQILQLLGHEAYSNNKLNTKYISKIVFNDKIMLNRLKAIVHPAVYADAEDWFKQHQ
                     NENYVLYESALLLVNGKNNLVDKVILVIADIESRVQRVIDRDKSDRNSVLQRIQSQSI
                     QENLIPFADFIVNNNQDYSQIEFQVLTIHNQLTN"
     gene            2045..2779
                     /locus_tag="PLF48_07310"
     CDS             2045..2779
                     /locus_tag="PLF48_07310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF49167.1"
                     /translation="MYLYTDWAACIPMANEETDFYPFVDMLTRVMDVIGTGKIYFVID
                     NASKDKTLEFARDLEKKDSRFEVVWAPDNKNVVDAYLAGFRIAYDRGHDIIIEMDAGM
                     SHDPRAIPMYLRVLNEGNEVAFGSRFIKDGSMGDSPFNRRMLSKSGTVLANILLGTKL
                     FDMTSGFQGFHREIIGKLLQYPLKSRAHFYQTEVKYLLRKHRTAEVPIHYQAPSPRVS
                     PSAIKNARQTLMYYFIERLKGNAPTI"
     gene            2842..3849
                     /locus_tag="PLF48_07315"
     CDS             2842..3849
                     /locus_tag="PLF48_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HNF49168.1"
                     /translation="MSVKSLIITSIAPDTLEVLHTYAKECTDRNIRFICIGDTKSPEN
                     FNIEGCDFWSVDRQLELDSKFAKICPTRHYARKNIGYIIALQNGTTELVETDDDNLPR
                     AEFWHEKSKSVKAHAVKETDWINVYKYFSDSFIWPRGLPLEELQKPTPSLLEFPVQDI
                     LCPIQQGLADENPDVDAVYRLTYPLPLNFKKGLRLALGNNAWSPFNSQNTYWYKEAFP
                     LLYLPAYCSFRMTDIWRSYVAQRIAWANGWSILFHEATVWQERNAHNLLKDFEDEIPG
                     YLNNAKICSELTALPIKGGLENILDDLLLCYEMLTEKGFVGKEEMPLVKAWCDDITPY
                     L"
     gene            complement(3881..5146)
                     /gene="bioA"
                     /locus_tag="PLF48_07320"
     CDS             complement(3881..5146)
                     /gene="bioA"
                     /locus_tag="PLF48_07320"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNF49169.1"
                     /translation="MNYSEIDKQNIWHPFTQHQTAGDQLCIVYADGCYLEDEQGNRYL
                     DAISSWWTNLFGHRHPYITQSIKNQLDTLDHVLFAGFTHPAAVELSYKLLKFLPSNQA
                     KIFFSDNGSTAVEVALKMAFQYWYNKDMPKTRIVAFKHAYHGDTFGAMSVGARGVFNR
                     PFESLFFEVDAIDLPSDENFQAVINEFEHILSRGDVAAFIFEPLVLGSAGMKMYNARH
                     LDELLSIAAKYDVLCIADEVMTGFGRTGKYFATDYLKHKPDILCMSKGITGGFMPLGV
                     TSCTVEIYSAFLSTDKLKTFFHGHSYTAYPLACAAAAATMDVLEKEWQMVEQLCKWQA
                     AHVLQLAKHKKVAQIRQTGTIVAFDIKSGSTADYFNEIRDKFYAEILKKGVILRPLGN
                     TVYIMPPYTINSEELQFVYNQINEVLDIL"
     gene            complement(5143..5760)
                     /gene="bioD"
                     /locus_tag="PLF48_07325"
     CDS             complement(5143..5760)
                     /gene="bioD"
                     /locus_tag="PLF48_07325"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNF49170.1"
                     /translation="MSKNFFVTGIGTDVGKTVGAAVLVQALKANYWKPIQAGNLEFTD
                     SDFIRQNTDYAKLVMDESVLLSTPASPNIAAKFESKEIDVQRIEIPYVINNFIVEGAG
                     GIMVPINDKHSMLDLINKIQFPVILCVKSYLGCINHFLLTVEVLKLNKIPISGIILSG
                     TFDSEVKETILSFTDLPLIVHIPFTDNLSSEFIREQAEHINLDLL"
     gene            complement(5753..>6659)
                     /locus_tag="PLF48_07330"
     CDS             complement(5753..>6659)
                     /locus_tag="PLF48_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNF49171.1"
                     /translation="KLAKFYHAEAALLFNSGFDANIGLIATIARKGDIIFYDELVHAS
                     IQQGMLLSGAHLVSFCHNDIEDLKAQLEQIGNHQARFLITESLFSMQGDIAPLHQLAQ
                     LAKDAELNFIVDEAHANGIFGSLGSGLCNRDEIEEICFARVFTFGKAIGSHGGVVVGS
                     KVLKDYLINFSKPFIYSTALNLADLLSVEHAYFLLQNNINQQLKINNLILYFNKRIRQ
                     KKLSQFITGDGPVYGFYVKGNENCRQAAAYLQTNGFDIRPILSPTVPKGKERLRIILH
                     SFNKTDEIAILTEKLLDIQNIIPNE"
BASE COUNT         2146 a         1195 c         1192 g         2126 t
ORIGIN      
        1 tatcttcgtg ttatcattat ggagctatcg cgcatagccg accacattgt ttgtgatacg
       61 gtaattgccg tggacaccgg agcaatgacc ggatttttgc atttgttcca ataccgtgaa
      121 tggatttatg aaatatttga agaaatctgc ggaagcaggc tgactaccaa tattggcaga
      181 ataggtggtt tgaatgctga tttttctcaa caaacctggg ataaaatcaa tcattttatg
      241 gcagaatttc caaaaggatt aaaagaattc cagaaattag tggacagaaa ccgcattttt
      301 atggacagaa ccattaatgc aggtcccatt actgctgaaa aagcattggc ttacggtttt
      361 accggaccaa atctgcgtgc tgccggagtt gattatgatg ttcgtgtagc caatccttat
      421 tcgtcttatg aagatttcac ttttgagatt ccaattggca ccaatggaga tacttatgac
      481 cgttatatgg tacgcatgga agaaatgtgg caaagctatt ccattgtgga acaagccatt
      541 aaaaatcttc cggacggcaa accacatgca gatgtacccg atttcttttt gccgcctaaa
      601 gaggcagttt acaataatat ggaagccctg atttaccatt tcaaaatcgt aatgggtgaa
      661 actgatattc cgaaaggaga ggtgtaccat gcagtagaag gaggaaatgg ggagttggga
      721 ttttatctca tttcagacgg tggacgcaca ccattccgtt tacatttccg tcggccttgt
      781 tttatttatt accaggctta tccagaaatg ataaagggag ccatgctcag tgatgccatt
      841 ttaacgatga gcagcatgaa tgtgatagcc ggtgagttgg atgcttgata attttatggt
      901 tattcactaa agaactccat attttcacca caatattttg aataatgtca tttagacagt
      961 agatttgcaa actaaacaga atttatgatt ttagcatttt tcttacaagc agcacctgct
     1021 gcaggcggaa atagcccgat gtctttcatc tttatgctct gcttaatgtt cgttgtgttt
     1081 tatttctttt taattcgtcc gcaacaaaaa aagcaaaaag aagctcagga gttttcgaat
     1141 aatatgaaaa taggggacag gattatcacc attggtggtg tacatggcaa aattgtagcg
     1201 attaacgatg acaatacata taccattcag gttgacggac caacaagatt aaaaattgaa
     1261 aagtctgcag ttaatgcaga ggcatctaaa gctttacagc aaaacaccgg cacaaatgct
     1321 taatttttaa gtacttatgt taaaagttgg aatcactggc ggaattggtt caggtaaaac
     1381 aacagtttgt aaaatctttg aactattagg catttctgtt tattattcag atttaagagc
     1441 caagcaatta atgacagaaa atcagttagt taagcaacaa atacttcaac ttcttggaca
     1501 cgaggcttat tcaaataaca agttaaatac caagtacatt tctaaaattg tatttaatga
     1561 caagattatg ttaaacagac tgaaagccat tgttcatcct gctgtttatg ctgatgctga
     1621 agattggttt aagcaacatc aaaatgaaaa ttatgtgctt tatgaatctg cattactgtt
     1681 ggttaatggc aagaacaatt tagttgataa agtcatttta gttatagcag atatagaatc
     1741 acgtgtacag cgtgtgatag acagagataa atcggataga aattctgttt tacaaagaat
     1801 ccaatctcaa tcaattcaag aaaatttaat accattcgca gattttatag ttaacaataa
     1861 tcaagattat agtcaaattg aatttcaagt attaactatt cataatcaac ttactaacta
     1921 aatttctatt aatgtttttg tcctataatt tacattatgt taaatagagt attttaaaaa
     1981 aaaattattt cattaattta gtacttcttt aatattttag tatttttgca acatatatcc
     2041 tgctatgtat ctctatactg actgggctgc ttgtattcca atggccaacg aagaaactga
     2101 cttttatcct tttgtcgata tgctcacaag agtaatggat gtgataggca ccggtaaaat
     2161 ttattttgta atagataatg cttctaaaga caagacgctg gaatttgctc gtgatttgga
     2221 aaaaaaagac agtagatttg aagtggtttg ggcaccagac aataaaaatg tagttgatgc
     2281 ctatttagca ggttttcgta tagcttatga ccgcggacat gatattatca ttgaaatgga
     2341 tgcaggaatg agccacgacc ccagagctat tcctatgtat ttgcgggttt taaatgaagg
     2401 aaatgaagtt gctttcggtt cccggtttat aaaagacggc tccatgggcg actctccttt
     2461 caaccgtaga atgttatcaa agtcaggtac agtattagcg aatatcttac ttggcactaa
     2521 actttttgat atgacttccg gttttcaggg tttccatcgc gaaattattg gaaaactgtt
     2581 gcaatatcca ctgaaatcca gggcgcattt ttatcaaact gaagtaaaat atttgttgcg
     2641 caaacaccgt acggctgaag tgccaataca ttatcaagct ccatcaccac gtgtatctcc
     2701 ttctgcaata aaaaatgcac gtcaaacttt aatgtattat tttattgaaa gattaaaagg
     2761 aaatgctcca actatttaac ataatacttt gaaattaatt atagcaagat tataaactac
     2821 tttaaatcaa taaaataaat catgtcagtt aaatcattaa ttataacatc aattgctcct
     2881 gacactttag aagtattaca cacttatgcc aaagaatgta ctgatagaaa tatccggttt
     2941 atttgtattg gagatactaa atcacctgaa aattttaata ttgaaggatg tgatttttgg
     3001 tcagtagacc gtcaacttga acttgactca aaatttgcaa agatttgtcc tacaagacat
     3061 tatgcaagaa aaaacattgg ctatattatt gctttacaaa atggaactac ggaattagta
     3121 gaaactgatg atgataattt acctcgtgct gaattttggc atgaaaaatc taaatctgta
     3181 aaagctcatg ctgttaaaga aactgattgg ataaatgtgt ataagtattt cagtgacagc
     3241 tttatatggc caaggggttt gcctttggaa gaactgcaaa aaccaactcc ttccctatta
     3301 gagtttccag tacaagatat actttgtcct atacaacaag gtttagctga tgaaaatcct
     3361 gatgtggatg ctgtttatcg tttaacttat cctctgccct taaattttaa aaaaggtttg
     3421 agacttgctt tgggcaataa tgcatggtca ccatttaaca gccaaaatac gtattggtat
     3481 aaagaagcct ttccactttt gtatttaccg gcttactgca gctttcgtat gacggatatt
     3541 tggcgcagtt atgtagcaca acgcattgcc tgggctaacg gctggagtat tttgtttcat
     3601 gaagcaacag tatggcaaga acgtaatgcc cataatttat tgaaagattt tgaagacgaa
     3661 atacccggtt atttaaataa cgctaagatt tgcagcgagt taacagcatt gcctataaaa
     3721 ggtggcttag aaaatatact ggatgatttg cttttatgtt atgaaatgct gacagaaaaa
     3781 ggttttgtcg gtaaagaaga aatgccgctt gtcaaagctt ggtgtgatga tattactcct
     3841 tatttataaa gctattcatt tcttttagac tttttctgat ttagagaata tctaatacct
     3901 catttatttg attataaacg aattgcaatt cctcactgtt gattgtatac ggtggcatta
     3961 tataaacagt attgcccaac ggacgcaata tgacaccttt ttttagaatt tctgcataaa
     4021 atttatcccg tatttcatta aaataatctg ccgtactgcc acttttaatg tcaaatgcta
     4081 caatagttcc ggtttgcctg atttgtgcta cttttttatg tttagctaac tgcaaaacat
     4141 gtgctgcttg ccatttacaa agttgttcaa ccatttgcca ttctttttcc aaaacatcca
     4201 tggtagccgc agccgcagca caagccaaag gataggcggt gtaagaatgc ccgtgaaaga
     4261 aggttttaag cttgtctgtg cttaaaaatg cagaataaat ttccacagtg caagaagtta
     4321 cacctaatgg cataaatcct ccggtaatac cttttgacat gcacaatata tcaggtttgt
     4381 gtttcagata gtctgttgca aagtactttc ctgttctgcc aaaacccgtc ataacttcat
     4441 cagcaatgca cagtacatca tattttgctg caatcgaaag aagttcatcc agatgtctgg
     4501 cattatacat tttcattccc gccgaaccca ataccaaagg ttcaaatata aatgcggcca
     4561 catctccacg acttaaaata tgttcaaatt catttattac ggcttgaaaa ttctcatccg
     4621 atggcaaatc aatggcatct acctcaaaaa atagggattc aaatgggcga ttaaaaaccc
     4681 cacgagcccc tacactcatt gcaccaaagg tgtcgccatg ataagcgtgc ttaaaagcca
     4741 ctatacgggt tttaggcata tctttgttgt accagtactg gaaagccatc tttaaagcca
     4801 cctctacggc agttgagcca ttatcagaaa agaaaatctt agcctgattt gaaggaagga
     4861 atttgagtaa tttgtatgaa agttcaactg ctgccgggtg ggtaaaacct gcaaataaca
     4921 cgtgatctag tgtatctagt tggtttttaa tactttgtgt aatataagga tggcgatgac
     4981 caaataaatt cgtccaccag ctcgaaatcg catccagata acgatttcct tgttcatctt
     5041 caaggtagca accatctgca tatacaatac atagctgatc tccggcagtt tgatgctgcg
     5101 taaaaggatg ccagatgttt tgtttgtcta tttctgaata gttcaaagca aatcaagatt
     5161 tatatgttca gcttgttctc tgataaattc acttgacaaa ttgtccgtaa atggaatatg
     5221 tacaattaat ggtaaatctg tgaaacttaa tatcgtctct ttaacctcac tatcgaaagt
     5281 accactcagg ataatgccgc ttatgggtat tttatttaat ttcaatacct caacggttaa
     5341 caagaagtgg ttaatacaac ctagataact tttaacacac agaataacag gaaattgaat
     5401 cttattaatt aaatctaaca ttgaatgttt atcattaatt ggcaccatta ttccccctgc
     5461 tccttctact ataaagttat tgattacata aggaatttca attctttgca catcaatttc
     5521 ttttgattca aatttagctg caatattggg agaagccggt gtagaaagca acaccgattc
     5581 atccatgact aattttgcat aatccgtgtt ttgccggata aagtcagaat ccgtaaattc
     5641 caggttgcca gcctgaatgg gtttccaata gttagcctta agtgcctgaa ccaaaacggc
     5701 agcgcccact gtcttcccga catctgtacc aataccggtt acaaaaaaat ttttactcat
     5761 taggtataat attttgaata tcaagcagtt tttcagtcaa aattgctatc tcatctgttt
     5821 tattaaaact gtgcaatata atcctaagcc gttcttttcc tttcggcaca gttggtgata
     5881 aaataggtcg aatatcaaac ccatttgttt gtaagtaagc cgcagcctga cgacaatttt
     5941 cattgccttt tacatagaaa ccataaaccg ggccatcacc ggtgataaat tgactaagtt
     6001 ttttttgtct gatcctttta ttaaagtata aaattaaatt attaatctta agttgttgat
     6061 ttatattgtt ttgaagtaaa aaatatgcat gttccacgct tagtaaatca gccagattca
     6121 atgccgttga atagataaaa ggcttactga aatttatcag atagtccttt aatactttgc
     6181 ttccaactac tactccccca tgcgatccta tggcttttcc gaaggtaaat actcgagcaa
     6241 agcagatctc ctcaatttca tcccgattac ataaaccact acccagactg ccaaaaattc
     6301 cgtttgcatg ggcttcatca acaatgaagt ttaattcagc atcttttgct agctgtgcca
     6361 actgatgcaa tggagcaata tcaccttgca tggaaaaaag tgattctgta attaaaaatc
     6421 gagcttgatg attgccaatt tgttctaatt gtgctttcaa atcttcaata tcgttatgac
     6481 aaaaagagac taaatgagct cccgaaagta gcatgccttg ttgaatggaa gcatgtacca
     6541 attcatcata aaaaataata tctcctttac gggctatagt agctatcaaa ccaatattgg
     6601 catcaaaacc ggagttaaaa agcagtgctg cttctgcatg gtaaaattta gccagtttg
//
LOCUS       DAOQII010000130         6630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6462_length_6630_cov_4.937186, whole genome shotgun sequence.
ACCESSION   DAOQII010000130 DAOQII010000000
VERSION     DAOQII010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_6630_cov_4.937186"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PLF48_07335"
     CDS             <1..1275
                     /locus_tag="PLF48_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF49172.1"
                     /translation="VWTVIDTANGCVVMDTVEINYLKCPGITDIIYSKTKSNAICRNN
                     GEINIQIIQSSLPVSYLWNTGDTAHQLSGLDSGMYIVKIYDRSSCTEDIYDTTFIKAS
                     HKSYATIHPFICIGDTLKLNQHSYYANGTYYDTLVNSLGCDSILTIQLTTYPVYHVYD
                     SVSICHGDNYTLPNGQIISQAGNYQISYTSLNGCDSIYHYYLSLKPLSSTNIDTSLCY
                     DAYYILPNGDTILQAGKYVDTLQNSIGCDSIIQFQISFLPKLNKPQLGEDTIICMDDA
                     WTIALTYPAYVHYVWQDGSHLANYTATKKGIYWLQVTDGCTIERDTLKLGLKSCDCHF
                     YIPEAFSPNHDGFNDLFGPIEKCEFYSDYTFEIFNRWGELLFSSPNPQIMWDGLFKNS
                     PQPLDEYIWTLKYTETRTGEKIFTKGTITLLR"
     gene            complement(1277..2509)
                     /locus_tag="PLF48_07340"
     CDS             complement(1277..2509)
                     /locus_tag="PLF48_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNF49173.1"
                     /translation="MSEQKVKCPNCGSVFPIDELLSHQAEEKYRLAFEEKMKAQQHVF
                     ATQEKEFASKQAAFEEKRLKAQEEYLQKLEQDRERIKKEEQEKTSKLIREEFELKLKN
                     QEDELSARKRENEQLKQKELDFLKKQEALLEKEKEIELHIQRQLNTERERLAEKISKQ
                     EQDKNDQKLKELQFKLEQQVKLADEFKRKAEQGSMQIQGEIQELELEAILKEAFPFDH
                     IDEVGKGVKGADCIQTVRNQFGQICGKIIYESKKTQNFANDWIEKLKTDLRAQQADIA
                     VIVTQVMPKDMSQFGEKNGVWICSFVEVKALAFILRHSLIKVSAALKSQENKGDKMQV
                     LYDYLTGNEFGEQWKAIREGFLGLKISIQKERDAMEKLWKAREVQLQKILLNATHISG
                     SIEGISGLENVDIHLLDE"
     gene            2704..4017
                     /locus_tag="PLF48_07345"
     CDS             2704..4017
                     /locus_tag="PLF48_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNF49174.1"
                     /translation="MIYSLFSKICLALLLGGVIGLERENSQGRDSEGIGGIRTYALVS
                     LLGALAGLFYNLHYEIISILTIIGFILLLLGNFLLEAFQSKDFGITSELSALITFLLG
                     LIIITEMVPLQITVTVFILLVFILSLKQKTEKFATVISQHELQSFISYAIIAVVVLPL
                     LPNESYQFKDIPALDNILTALNLDFNRFKDLEFINPRKVWFVVVLITGIDVFGYILGK
                     LLGEKRGFAVASFFAGFVSSTSATQSLAQRSKLTGLVFPFLGAALIANLASFIQIFML
                     IGPLNPKFLMSIIPGILFMILMAGILAIYFFRKDEAQVADTKIDNKPIFALIPALKFA
                     TLLIFVKLLTKVSLVVFGESGFIVSSVIASFAALDPIMVNLAEMAGKSITFKFAFITL
                     LIVNASNLMSKTVYSFIQGNRKFALYFFISALLIIGVSFIGLFFI"
     gene            complement(4003..4734)
                     /locus_tag="PLF48_07350"
     CDS             complement(4003..4734)
                     /locus_tag="PLF48_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49175.1"
                     /translation="MLKIKIIILSLLEMKSVTVLKILSVLLIILSVALMIINPQKEQN
                     LSEGFFTPIVAFEFIKTPAEVAHFFEVKHVYAYKSKMLLGNAVDYAFMLVYSLLLFGV
                     SRYFNAIRKSAVFRIASVLCLLMLLGDALENMQIYQIIQAEATTNISTNLYLLNIFTW
                     LKWASIASVFGLFATYYFRQNDLLSMLCGSAGIVSLILAVVAFLLQNGLWYELFALSV
                     VCNFLLTVIYIFTRKRALQKLLNKK"
     gene            4742..5359
                     /locus_tag="PLF48_07355"
     CDS             4742..5359
                     /locus_tag="PLF48_07355"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNF49176.1"
                     /translation="MNNRIKHTLFPVLVIVCILLAGRLYFYNMRDKANHEKVNSTIVV
                     ERIQKVMKLVAVESNFSELMNYNDFQYVDFPGFRKDALVKVNAKVSIGYNLDSLKIKT
                     NEKDKTIIIEHMPQPEVLSIDTDISFENLSEGWFNHFSKEELTKLNKLAKDKIKQHAL
                     APELVKQAEEQKRELFDLLFYMAKENGYKIISEGKVLTPIQNLNQ"
     gene            5362..5853
                     /locus_tag="PLF48_07360"
     CDS             5362..5853
                     /locus_tag="PLF48_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNF49177.1"
                     /translation="MELSIAITIGIILLVVGLVSCVLPPLPGPPIAYLSLVLIYFVKD
                     RPADLPLWLMITYAVLTILVLIVDNFIPIWGTKQFGGTKAGIRGSFIGILVGIFLSPF
                     GGFSILLCPFLGAMIGELFVGQPLNVALKSGMGSFIGFLLTSGLKIILVVFMGLHFFK
                     AAF"
     gene            5955..>6630
                     /locus_tag="PLF48_07365"
     CDS             5955..>6630
                     /locus_tag="PLF48_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49178.1"
                     /translation="MTKFILKFVFMCFAVVVFAQKTNKTTSTAPTKTSTTKPASTPPS
                     QTTTKAPATTTTAPAKCVPVAGKKTLAFTGSFLMNFTINENGTSKSGKIFYAFDCYQT
                     CMIPTFDLKTNADYKMRAITDISEGTITMLTEDTKGKKSGLIFNIPKKVITANANTVK
                     FTTQKTTETKVIEGYTCTKWITNGDNGVVIVSWISDKITLNYKDMLSSIGQSFKGKSP
                     FENSYAK"
BASE COUNT         2061 a         1196 c         1123 g         2250 t
ORIGIN      
        1 gtatggactg taatagatac tgcaaatggc tgtgtcgtaa tggatactgt agaaataaat
       61 tatttaaaat gtccggggat tactgatatc atttattcga aaacaaaatc aaatgccatt
      121 tgccgaaaca acggtgaaat aaatattcaa atcattcaat catcactacc tgtcagttat
      181 ttatggaata ccggcgatac agcacatcag ttatccggtt tggattcggg tatgtatatc
      241 gtaaaaattt atgacagaag ctcatgtacg gaagacattt atgataccac gtttattaaa
      301 gcttcacata aaagttacgc aacaattcat ccatttattt gcattggtga tacactgaaa
      361 ttaaatcaac atagttatta tgcaaatggt acttactatg atacacttgt aaatagtttg
      421 ggttgtgaca gtattttaac catacaatta acaacatatc ctgtttatca tgtttatgat
      481 tctgtttcta tttgtcatgg tgataattac actttgccta atggtcaaat catctcacaa
      541 gcaggtaatt atcaaatcag ttataccagc ttaaatggtt gcgacagcat ttatcattat
      601 tacttgagtt taaaaccttt gagcagcacg aacatagata ccagcttatg ctatgatgct
      661 tattatatct tacccaatgg agatactatt ttgcaagctg gaaaatatgt ggacaccttg
      721 caaaactcaa taggctgtga cagtattatt caatttcaaa tttcctttct gcctaaacta
      781 aacaaaccgc agctaggaga agatactata atctgtatgg atgatgcttg gacaatagca
      841 ttgacttatc cggcatatgt acactatgtt tggcaagacg gcagtcattt ggccaattac
      901 accgccacaa aaaaaggcat ctactggctg caggtaactg atggctgtac gatagagcgt
      961 gatactttaa aactaggatt gaaatcgtgc gattgtcatt tttatatccc ggaagccttt
     1021 agtccgaatc atgacggctt taatgactta tttggtccca ttgagaaatg cgaattttat
     1081 tcggattaca cttttgaaat ttttaatcgt tggggagaat tattattttc ttcaccaaat
     1141 cctcaaatca tgtgggatgg tttatttaaa aattcacctc aacctttgga tgaatatatc
     1201 tggactttaa aatatacgga aacaagaaca ggtgagaaga tttttaccaa aggtacgatt
     1261 acgcttttgc gttaagttat tcatccagca aatgtatatc tacattttcc aatccggaaa
     1321 taccttcaat cgaaccggag atatgggtgg cattcagtaa aattttttgc aattgcactt
     1381 ctcgggcttt ccacaatttt tccatggcat cccgttcttt ttggatggaa attttcagac
     1441 ccaaaaatcc ttctcgaatg gctttccact gttcaccaaa ttcgtttcca gtcaagtaat
     1501 catataatac ctgcatttta tcacctttgt tttcctgcga ttttaatgca gcagaaactt
     1561 ttattaacga atgtcgtaag ataaaagcca gtgctttaac ctccacaaaa ctgcaaatcc
     1621 aaaccccgtt tttttcgccg aattgtgaca tatctttagg cattacttgc gtcactataa
     1681 ctgctatatc ggcttgttgt gctcttaaat ctgtctttag tttttcaatc cagtcgttgg
     1741 caaaattttg tgttttcttg ctttcatata taattttacc gcaaatttga ccaaactgat
     1801 tcctcaccgt ttgaatacaa tcggctcctt ttacaccttt gcctacctca tcaatatggt
     1861 caaacggaaa tgcttctttt aaaattgcct ccagttccag ttcttgtatt tcgccctgaa
     1921 tttgcataga accctgctcg gctttgcgtt taaattcatc cgccagtttt acttgctgtt
     1981 ccagcttaaa ttgtaattct ttaagcttct ggtcgttttt atcttgttct tgcttgctta
     2041 ttttttcagc caatcgttca cgttctgtat tgagttgacg ttgtatgtgt aattctattt
     2101 ctttttcttt ttcgagcagt gcttcctgct ttttaagaaa gtcgagctct ttttgtttta
     2161 actgttcatt ttctcttttg cgggcactca attcatcttc ttgatttttg agtttaagtt
     2221 caaattcttc acggattaat ttactcgtct tttcctgttc ttctttttta atgcgctctc
     2281 ggtcttgttc cagtttttgt aaatattcct cttgtgcttt caatcttttt tcttcaaaag
     2341 ctgcctgttt ggatgcgaat tctttctctt gcgttgcgaa aacatgctgt tgtgctttca
     2401 tcttttcttc aaaagctaaa cgatattttt cttccgcctg atgactcaac aattcatcaa
     2461 taggaaaaac actcccacaa ttcggacatt ttactttttg ttcactcatt ttacaaaatt
     2521 aagaaaagct gattaagcat gataaaagaa tacaaaaaat ataaggataa ataaacttat
     2581 ttttcacaaa tttagtaccc aacatttcca tgcaatttat tccacttaaa aaccaaatct
     2641 acaaacctga ttttaggatt tgatatacgt attttgtaaa tttgcactac acagatgtat
     2701 tagatgattt acagtttatt ttcaaaaatt tgtctggctc ttttattagg tggagttatc
     2761 ggtttagaac gcgaaaattc acagggacgt gactctgagg gtattggcgg tattcgcaca
     2821 tatgccttag tttctttatt gggggcttta gcagggttgt tttacaattt gcattacgaa
     2881 ataatttcca ttctcactat tatcggtttt atcttgctct tgttgggtaa ctttttactg
     2941 gaagcatttc aaagtaaaga tttcggcatt accagtgagc tttctgcttt aattacattt
     3001 ttattagggc ttattatcat tacagaaatg gtacccttgc aaattactgt aacggttttt
     3061 atcctgttag tatttatcct ttcattaaaa cagaaaactg aaaaatttgc tacggttatt
     3121 tcgcagcatg aattacaatc atttatcagt tatgctatta ttgcagtagt tgtacttccg
     3181 ttattgccaa atgaaagtta tcagtttaaa gatattcctg cattagacaa tatactgaca
     3241 gcattgaatt tagattttaa ccgttttaaa gatttagaat ttattaaccc gcgcaaagtt
     3301 tggtttgtag tggtattaat tacgggtatt gatgtattcg gatatatatt gggtaaatta
     3361 cttggagaaa agagaggttt tgccgtagcc agtttttttg ccggtttcgt ctcctctaca
     3421 tctgctacac aatcgcttgc acagcgcagc aaattaactg ggttggtttt tccgtttttg
     3481 ggtgcagcac tgattgccaa cttagccagt tttatacaaa tcttcatgct aattggtcca
     3541 ttaaatccaa aatttttaat gagtataata cccggtatac ttttcatgat tttgatggca
     3601 ggcattttag ccatatattt tttcaggaaa gatgaggcac aggttgcaga tactaaaatt
     3661 gataataagc ctatctttgc tttgattcca gcactgaaat ttgctacatt gctcatcttt
     3721 gttaaattgc ttactaaagt tagtttagtc gtttttggcg agtctggttt tattgtcagc
     3781 agtgtgattg cctcttttgc cgccttagat ccgataatgg tgaaccttgc agaaatggcc
     3841 ggaaaatcca tcactttcaa atttgcattt attacactgc ttattgttaa tgcatcaaat
     3901 ttaatgagca aaaccgtcta ttcttttatt cagggaaacc ggaaattcgc actttacttt
     3961 tttatatcag ccttactcat cattggagtt agctttatcg gattattttt tatttagcag
     4021 cttttgcaat gccctttttc ttgtgaaaat ataaattacc gtcaataaaa aattgcagac
     4081 caccgataga gcaaaaagtt cataccacaa tccattttgt aaaagaaatg ccactacggc
     4141 aagaattaac gaaacgatgc ctgcactacc gcaaagcatg cttaataggt cattctgacg
     4201 aaaataataa gtagcaaaca atccgaatac agaagcaata cttgcccatt tcagccaggt
     4261 aaaaatattg agcaagtaaa ggttggtgct aatattggtt gtagcttcag cttgaatgat
     4321 ttgatagatt tgcatatttt caagggcatc tccaagcagc attaacaaac acaagacaga
     4381 tgcaatacga aacaccgctg attttcttat agcattaaaa tatcgtgaaa ctccaaataa
     4441 taataaggaa taaaccagca taaatgcata atctaccgca ttgccgagca acattttaga
     4501 tttataagcg taaacatgct ttacttcaaa aaaatgtgca acttctgccg gagttttaat
     4561 aaattcaaaa gcaactatag gtgtaaaaaa gccctcgctc agattttgtt ctttttgtgg
     4621 attaataatc atcagagcaa ctgacaaaat aatcagtaag acacttaaaa ttttcaaaac
     4681 agttacgctt ttcatctcca ataaagataa aataattatt tttatcttta gcatggacat
     4741 tatgaataat cgaattaagc atacgctgtt tcctgtttta gttattgtct gcattttgct
     4801 tgcaggcaga ttgtattttt ataatatgcg ggataaagcc aatcatgaaa aagtgaacag
     4861 tactattgta gtagaacgca tacaaaaagt gatgaaactg gtggctgttg aaagtaattt
     4921 ttcagagtta atgaattaca atgattttca gtatgtcgac tttcccggtt ttagaaaaga
     4981 tgcattggtc aaagtaaatg caaaagtttc catagggtat aatttggaca gccttaaaat
     5041 taaaactaat gagaaagata aaaccatcat tatagaacac atgccccaac ccgaggtatt
     5101 atcaattgac accgatattt ctttcgagaa tctgagcgaa ggctggttta atcatttttc
     5161 aaaagaagaa ctcaccaaat taaacaagct ggcaaaagat aaaataaagc aacatgcatt
     5221 agctccggag ttagtgaagc aagcagaaga gcaaaagcgt gaattatttg atttactctt
     5281 ttacatggca aaagaaaacg gttataaaat tatcagtgaa ggcaaagtac ttactccgat
     5341 tcaaaatctc aatcaataaa aatggaatta agcatagcca tcactatagg tatcatcttg
     5401 ctggtagtgg gattagtaag ttgtgtttta cctcccttgc cgggtccgcc aattgcttac
     5461 ttgtcattag tgctgatata ttttgtaaaa gacagacctg cagatttacc cctttggttg
     5521 atgattacat acgccgtgct gacgatactt gtgttaatag tcgataattt tataccgata
     5581 tggggcacca aacagtttgg cggcactaaa gccggaatac gcggcagctt tattggtatt
     5641 ttagtgggta tattcctctc tcctttcgga ggattttcca ttctcctatg tccatttttg
     5701 ggagccatga ttggtgaatt attcgttgga cagcctttga atgtggcctt aaaatcagga
     5761 atgggatcat ttatcggatt tctgctgacc agcggattaa agataattct tgtcgtgttt
     5821 atggggcttc attttttcaa agcggcattt tagttttcag ttaagttgaa ttatatttca
     5881 caaaaaaaac aacaattctt atctcctatt gttttaataa gagaatacaa ctacatttac
     5941 tttaaattag atttatgaca aaatttatat taaaatttgt attcatgtgt tttgccgtgg
     6001 tcgtatttgc acaaaaaaca aataaaacaa ctagtactgc tcccacaaaa acttcaacga
     6061 caaaacctgc ctcaacaccg ccatcacaaa caacgactaa agcacccgca acaactacaa
     6121 cagcacctgc taaatgtgtg cccgttgctg gcaaaaaaac attagctttc acaggcagtt
     6181 ttctgatgaa ttttacaata aatgaaaacg gcacgagcaa atcgggtaaa atattttatg
     6241 cattcgattg ttatcagacc tgcatgattc caactttcga cttgaaaaca aatgccgact
     6301 ataaaatgcg tgcaattacg gatatatccg aaggcacgat taccatgctt accgaagata
     6361 ctaaaggcaa aaaatcgggc ttgatattta atatccctaa aaaggtaatt actgctaatg
     6421 caaataccgt aaaatttacg acacagaaaa caacagaaac aaaagttata gaaggctata
     6481 cctgtacaaa atggataact aacggtgata atggagtagt gatagtttca tggatttcgg
     6541 ataaaattac attgaattac aaagatatgt tgagtagcat tggacaaagt tttaaaggaa
     6601 aatcgccatt tgaaaactct tatgccaaag
//
LOCUS       DAOQII010000131         6608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6493_length_6608_cov_4.535938, whole genome shotgun sequence.
ACCESSION   DAOQII010000131 DAOQII010000000
VERSION     DAOQII010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6493_length_6608_cov_4.535938"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..6602)
                     /gene="sprA"
                     /locus_tag="PLF48_07370"
     CDS             complement(165..6602)
                     /gene="sprA"
                     /locus_tag="PLF48_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNF49179.1"
                     /translation="MSRLPFVGAQTVINRIEVWVTNKTRQTENVRDVVCFQDIGEPNK
                     VYNPAILGGSGNAYPDNNSNGLYGIITHSADAANFRDPSRTISFLENKGFQAVDDYEK
                     TSARKLAISEYIINDKLGYISLNQQLRPDEVLGVAIEYTVNGKVYQIGEFATDLPPLA
                     DSTRAEDRVLALKMLKATSIRTTEPIWDLMMKNVYSLNAYNVSNEQFYFDIYYQDPGG
                     GLKRYLPEAGNISGQQLLKVLNLDNLNTQLDPQPDGRFDFIPNVTINPRNGRIYFPVL
                     EPFGKTLASKIGDPTISSKYEYRYLYDTTKTAALQYPEYNRFILKGSYRGTDNSTYRL
                     PGAFNLPQGSVTVSAGGNVLKENIDYTVNYGIGEINIINQGVLNSGVPIDIKFENNIL
                     FGVVNKSLIGTRLDYTINKNFSIGFTHLRLAEKPFTQKVNFNDDPIKNNVFGLDLNYS
                     TESKGLTRVFNKITAQDTKAPSKVNVMAEAAYFKPGHSKAINIDDAGTVYVDDFEGAS
                     TNFDIKGSFLSWQLASTPKNMPNPYGVEKFPESRLSDSISYGFNRGKISWYSIDPLLY
                     SKGNNNPMDDGAINDANSNIYTKQFQENDVFKYKENTTITNPPLYTLDLNFDPNKRGS
                     YNYETLPTAYSKGVKPNGDLRNPETRWGGIMRTIESTTDFEAANIEFVQFWVLDPFQF
                     PGADRKGNLYLHLGTISEDILKDSRKQYENGLPRPGAATTVDTSNWGVTPTITNALTN
                     QFDADPDVIRKQDVGLDGLDNDAERAFFGNYLATLNGVVSDANALTQAQNDPSTDDYA
                     FFKNDKFASSDDVRTRYFDFANTEGNSSNNAGSNNNINGNSKSQPDNEDLNNDNTLNE
                     TEEYFQYRIPFDLTSLQNSSYLSDRVRVDVNGNDSAIWYQFKIPIKEFESRVGNISDF
                     RSIRYIRLVLSDFDQPVTMRFAQISLIRNQWRRYDLSLDEPGEVLPGDNTTNTDFSVT
                     SVNVEDNSSRIPIPYAVPPGINREDNVSGYNNALQNEQAMSLQVCELKDGDARAVFKT
                     TDLDLRNYKRLKMFIHAEDYQGGAGSSFPIKNNDIHAFLRIGSDFTENYYEYDIPLKV
                     TPAGTYDINSDIDRRLIWPDSNDINIPLDSLTNLKQLRNNSNAPLTRPFEHFLADGKK
                     ATVMGNPDLGVASVMMIGIRNPKLIIGENDATDDGLSKCAEVWVNELRLAGFDESDGW
                     AALGRVDMQLGNLGNVILSGNMHTIGYGDLEQRLNQRYRDDYYQYDIATNLQLGKLIP
                     EKAGLQIPVYAQYSQTISNPQYDPYEYDTKLKDKYNAIKADGDLSKAEKKDKIDSIKR
                     VTQDVTTIASVNVTNLQKVRTNSEKKPRVYDVENFTLSYAYTQTQKHNPIVEDEKIVR
                     HRGSLGWSFAPASAYWQPFKKIKNNNIHLKPIKEFNLNFKPNSIQFRTDINRQYGYTK
                     IRDIGNDGLVILPTYDKYFTWDRFWSIKYNITKSINVDFMANNKARIDEPFGALDTKE
                     KRDTVKRNFWHFGRPINYNHIFNVSYNLPFQLIPALDWITVKTRYGATYDWLAAPLGL
                     TSLGNIIKNGQDYQITGEVNFKNLYAKSKQLKPYTTSSPPKDKKTYADDYAKYKDQMA
                     NLDKQIIQKKDEIVKKIDEIERAEKDTSFTKEEIKQLVDQKKQLKNDLRTLKLNKRQG
                     QMPANPKLEYVYRPLLMLQRASVSYDVKNTTILPGFMPSPLLFGQNFKQNAPGGAFLF
                     GAQRDTNWLNKIAQKGWMSTDTMINSQFIQTRQKTFNMRVTLEPYRDFRVEISMQKTK
                     GENYSEFFKQTGAGSAFQHLTPMVNGNMSMSFMMLRTIFDKVDQNNFSSTFHIFEQLR
                     EKYSQKFGEINLASVGPYQNDSIVLPNFKEGYGPFSQDVLVPSLLAAYSGRNPDNIKL
                     NPLKTMPLPNWRITYTGFSKTKWGKKLFTSFNIEHGYNCTFNVGSYVTNLKYLGLQGY
                     YGEDLYYVPEQLDSISGNFYSYYLIPQVVINEQFSPLIGIDITWKNSLITNFEFKKSR
                     TLGLSMLDYRLTETRSTEYSFALGYKLAKFKIPFKIKGKRITLNNDINMRADFSYRDD
                     RTLSYRLDQNIAEPTRGQKMISLAATIDYVINNKLNVRLFYDFRKTIPATLASYPTRS
                     HRGGITFRFSLTP"
BASE COUNT         1921 a         1254 c         1185 g         2248 t
ORIGIN      
        1 aatcagctgt ccgaaaacgg atgtaccgaa attatgcgta cttttattca tatagatgtt
       61 tgtgcaaaaa caaactatac aaattgagcc ataatcccaa aacgatggct ctcttttatt
      121 atttttatcg gacagtgtga tactttttct tgaatacaaa atttctaagg tgtcaatgag
      181 aatcggaatg tgattcctcc acgatgtgag cgtgtaggat aggatgccaa tgtagcaggt
      241 atagtttttc tgaaatcata aaacaaacgt acattcaatt tgttattgat tacatagtcg
      301 atggtagctg ctaaggaaat catcttttgt ccacgtgttg gctctgcaat attttgatcc
      361 aaacggtaac tcaaagtacg gtcatcccta taagaaaagt ctgcacgcat attgatatca
      421 ttattcaatg taattcgctt gcctttaatc ttaaatggta tcttgaactt tgccaattta
      481 tagcctaatg caaatgaata ttcagtacta cgggtttctg tcaaacggta atccagcata
      541 ctcaatccca atgtacgtga tttcttaaat tcaaaattgg taatcaaaga atttttccat
      601 gtaatatcaa ttcctattaa aggcgaaaat tgctcattta tgactacctg tggaatgagg
      661 taataactgt aaaaattacc cgaaattgaa tccagttgtt caggtacata atataaatcc
      721 tcaccataat atccctgcaa gcctaaatat ttcagattag ttacgtaaga accaacatta
      781 aacgtacagt tatagccatg ttcaatatta aacgatgtaa atagtttttt accccatttt
      841 gttttagaaa acccggtgta agtaatacgc cagttaggta aaggcatcgt tttaagtgga
      901 tttaatttta tattatccgg atttctgccg gagtaggccg ctaacaatga aggaactaac
      961 acatcctgcg aaaaaggacc atagccttct ttaaaattgg gtaaaacaat tgaatcattt
     1021 tgatacggac ctacagaagc taaattaatt tcaccaaatt tttgagaata tttctcccgt
     1081 aattgttcaa atatatggaa agtagagctg aaattatttt gatctacttt gtcaaatatc
     1141 gtacgaagca tcatgaatga catggacata ttgccattca ccattggagt caggtgttga
     1201 aaagcagaac ctgcacccgt ttgtttaaag aactcagagt agttttcgcc cttggttttt
     1261 tgcatggata tttcaactct gaaatcgcga taaggttcta atgtaacccg catattaaat
     1321 gtcttttgac gagtctgaat aaattgtgag tttatcatcg tgtccgtaga catccatcct
     1381 ttttgagcta ttttattcaa ccagtttgta tctctttgtg cgccaaataa aaatgctcca
     1441 cccggtgcat tctgtttgaa attctgacca aataaaagtg gagaaggcat aaagccgggc
     1501 agtatagtcg tgttttttac atcatagctg acagaagcac gttgcaacat taacaatgga
     1561 cgatatacat attccaactt cggatttgcc ggcatttgac cttgtcgctt attgagtttc
     1621 aatgtccgca aatcattttt cagctgtttc ttttgatcta ctaattgttt tatttcttct
     1681 ttcgtaaaag atgtgtcttt ttcagctcgc tcaatttcat ctattttttt tacaatttca
     1741 tctttctttt ggataatttg tttgtccaga tttgccattt gatctttgta tttggcataa
     1801 tcatcagcat acgttttctt gtctttagga ggagaagagg tagtataagg tttcagctgt
     1861 ttggacttgg catatagatt tttaaaattt acctctccgg taatctgata atcttgtccg
     1921 tttttaataa tattacctaa tgaagttaag ccaagcggag cagcaagcca atcgtatgta
     1981 gcaccatatc tggtttttac cgtaatccag tcaagtgcgg gtatcagttg gaacggtaaa
     2041 ttatagctta cattgaaaat atggttgtaa ttaattggcc ggccaaaatg ccagaaattg
     2101 cgtttcaccg tatcgcgttt ttctttagta tccaatgccc cgaaaggttc atcaatacgg
     2161 gctttgttat tagccatgaa atctacattg atggatttgg taatattgta ttttatactc
     2221 caaaatctgt cccaagtgaa atacttgtcg taagtaggca atatcactaa accgtcattg
     2281 cctatatctc tgattttggt gtagccatat tgtcggttaa tatcagttct gaattgtatt
     2341 gagttcggtt tgaaattcag attaaattcc ttaatcggtt taaggtgaat attgttgttt
     2401 tttatctttt taaacggttg ccaataagct gaagcagggg cgaagctcca tccgagagaa
     2461 cctctgtgac gaactatttt ctcatcttca actatcggat tatgtttttg agtttgtgta
     2521 taggcatagc ttagtgtgaa attttctaca tcataaactc ttggtttttt ttctgaattt
     2581 gtacgtactt tttgcaaatt tgttacattt acactggcaa tagtcgttac atcctgcgtt
     2641 actcttttta tcgaatctat cttatctttt ttctcagcct ttgataaatc accgtccgct
     2701 tttattgcat tgtatttatc ttttagtttg gtatcatatt cgtatgggtc gtattgcgga
     2761 ttggaaatgg tttgggaata ctgtgcatat accggaattt gcaaacctgc tttttcaggg
     2821 ataagcttac ctaattgcag atttgtagct atatcatact ggtaataatc atcgcgataa
     2881 cgttgattca acctctgctc caaatcgcca taaccaatag tatgcatatt gcccgataga
     2941 atgacattac ccaaattacc caattgcata tctacacgac ctaatgctgc ccatccgtca
     3001 ctttcatcaa acccggctaa gcgcaactca ttcacccaaa cttcggcaca tttagaaagt
     3061 ccatcatctg tagcatcgtt ttcaccaatt attaatttcg gatttcttat tcctatcatc
     3121 attacagatg ccacacccaa atcagggtta cccatcaccg tagctttttt tccatcagcc
     3181 aaaaaatgtt caaatgggcg ggtgagtggt gcattactat tatttcgtaa ttgttttaag
     3241 ttcgttaaac tatctaatgg tatattaata tcatttgaat caggccagat gagacgacgg
     3301 tcaatatcac tgttgatgtc gtaagtgcct gcaggcgtta cttttaatgg aatatcatat
     3361 tcgtaatagt tttcggtaaa gtcggaacct attcttaaaa atgcgtgaat gtcattgttt
     3421 tttattggga aagaagagcc ggctccgcct tgatagtctt cggcatgtat aaacattttc
     3481 agacgtttat aatttcgcaa atccaaatca gtagttttaa atacggcgcg ggcatctccg
     3541 tctttcaatt cacaaacttg caaactcatc gcttgctcat tttgcaaagc attattataa
     3601 ccactcacgt tgtcttcgcg attaataccc ggaggaacag catagggtat aggaatccgt
     3661 gatgaattat cttctacgtt tacagaggta acactgaaat cagtatttgt ggtattatcg
     3721 cccggcaaaa cttcacccgg ttcatccaat gataaatcat agcgtcgcca ttggtttctg
     3781 ataagactaa tttgtgcaaa acgcatagta acaggctggt caaaatctga taaaactaaa
     3841 cgtatatagc gaatagaacg aaaatctgag atattaccga cacggctttc aaattctttt
     3901 ataggaattt taaactgata ccaaatagcc gagtcatttc cattaacatc tactcttact
     3961 ctatcagata aataagaaga attttgtaaa ctggttaaat caaaaggaat gcgatattga
     4021 aaatactctt ctgtttcatt caatgtattg tcattattta aatcttcatt atccggctgg
     4081 ctttttgaat tgccgtttat attattatta ctgccagcat tattggatga gttaccttct
     4141 gtgtttgcaa aatcaaaata acgggtacgt acatcatccg aagaggcaaa cttatcattt
     4201 ttaaagaagg cataatcatc agttgatggg tcattttgag cttgtgtcaa agcattggca
     4261 tcagaaacaa ctccatttaa tgtggctaaa taattaccga aaaatgctct ttcagcatca
     4321 ttatccaagc catccagacc aacatcttgt tttctgataa catcgggatc tgcatcaaac
     4381 tgattggtca atgcattagt aatagttggt gtaacgcccc agttagacgt atctacagtt
     4441 gtagcagcac ccggacgcgg taatccgttt tcgtattgtt tgcgtgagtc ttttaaaata
     4501 tcttcggata tggtacctaa atgcaaatac agattccctt ttctgtctgc tcccgggaat
     4561 tgaaacggat ctaacaccca aaattgaaca aactcaatat tggccgcttc aaagtctgta
     4621 gtagactcaa tcgtacgcat gatacctccc cagcgtgttt cgggattgcg caaatcgcca
     4681 ttaggtttca cccctttgga atatgcggta ggtaatgttt cataattgta agatcctctt
     4741 ttgttagggt cgaaatttaa atccaatgta taaagcggcg gattggtaat cgttgtattt
     4801 tccttatatt taaacacgtc attttcctga aactgcttag tgtagatatt gctgttagca
     4861 tcattaatag ctccatcatc cattgggttg ttattgcctt tggaatacag caatgggtca
     4921 atggaatacc atgagatttt acctcggtta aaaccatatg agatactgtc acttaatctg
     4981 gactcaggaa atttttctac tccatatgga ttgggcatat ttttaggcgt agaagctaat
     5041 tgccaggaca agaaacttcc tttaatatca aaattagtac ttgctccttc aaaatcatct
     5101 acataaacag ttccggcatc atcaatattt atggctttgg aatgtccggg tttaaagtaa
     5161 gctgcttcag ccattacatt tactttagat ggtgctttgg tatcttgagc cgtaatttta
     5221 ttgaaaaccc gggttaatcc ttttgattct gtagaatagt ttaagtccag cccgaataca
     5281 ttatttttta tcgggtcatc attgaagttt actttttgtg taaatggctt ttccgctaag
     5341 cgcaaatggg taaagccaat ggaaaagttt ttatttatgg tataatccaa tcgtgtaccg
     5401 atcaaagatt tattaactac tccgaaaagg atattgtttt caaattttat atctatagga
     5461 actcctgagt tcaaaactcc ttggttgata atgttaattt cgcctatgcc gtaatttacc
     5521 gtatagtcaa tattttcttt cagcacgtta ccgccggcac taactgtaac agagccttgt
     5581 ggtaaattaa atgcgcccgg caatctgtat gtgctgttat ctgtgccacg atatgaacct
     5641 ttcagaataa agcggttgta ttcgggatat tgcaaagctg ccgttttggt cgtgtcatat
     5701 aaatagcgat actcatattt ggaagaaatc gttggatcgc caatttttga tgctaatgtt
     5761 ttaccaaatg gctctaaaac ggggaaataa attctaccgt tgcgcgggtt aatcgtaaca
     5821 tttggaataa agtcaaatct accatctggt tgtggatcta gttgtgtgtt gagattatcc
     5881 agattgagta ctttcaataa ttgctgacca ctgatattgc cggcttccgg taaatagcgt
     5941 tttaatccac cacccggatc ctgataataa atatcaaaat aaaattgctc attacttacg
     6001 ttatatgcat tcagcgaata cacattcttc atcatcaaat cccaaatagg ttcggtcgtt
     6061 ctgatagaag tggcttttaa catttttaag gcaagaacac gatcttcggc tcttgtcgaa
     6121 tctgctaatg gaggtaagtc agtcgcaaat tcacctatct gatacacttt tccattcact
     6181 gtgtattcaa tagcgacacc cagtacctca tccggtctca attgttggtt gagtgagatg
     6241 taacccagtt tatcgttgat aatatattcc gatattgcca attttcttgc acttgttttc
     6301 tcgtaatcat ctaccgcctg aaacccttta ttttctaaaa atgaaatagt acggcttgga
     6361 tctctgaaat tagcagcatc tgcactatgt gtaataatgc cataaagtcc attagaatta
     6421 ttatcaggat aggcattgcc tgagccgccc aatatggcag ggttatatac tttgttaggc
     6481 tcacctatat cctgaaaaca aacaacatcg cgcacatttt ctgtttgtcg tgttttgttg
     6541 gttacccata cttcaatacg gtttatcacg gtttgcgccc ctacaaaagg cagtctgctc
     6601 aagctggg
//
LOCUS       DAOQII010000132         6605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6499_length_6605_cov_4.058015, whole genome shotgun sequence.
ACCESSION   DAOQII010000132 DAOQII010000000
VERSION     DAOQII010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6499_length_6605_cov_4.058015"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2749)
                     /locus_tag="PLF48_07375"
     CDS             complement(<1..2749)
                     /locus_tag="PLF48_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF49180.1"
                     /translation="MRKNVLLLLGFMVMGFALFSGTKTPRPASVSQVHNGIQRCITKM
                     PGEEWENAFQQQIQQFQAAHSNPASRISVAYTIPVIVHVVYYTSAQNIPQSQVQSQIN
                     TLNADYAGTGLNVGNCPAVFQSLVANTNITFCLAAKDPNGVTLLEPGIDRVNAVTKGF
                     TNPGTSGWTDTYIDANIKPNTIWDPTKYLNIWVLPISGGILGYATFPAGTGLSGLSGF
                     GTATDDGVVINYTNFGTVGTGAYSKGRTASHEIGHWLGLRHISGDSNCGNDYCGDTPV
                     QSGNAGDGQGLNYGCPTYPSVTCSNGPNGDMFMNFMDYTDDACMYMFTPNQTTRMQTA
                     LANGTYRKFFSASTCNAGPSAPVANFTSNKTTVCVGGTVAFTDNSSNTPTSWSWTFAG
                     GTPATSTLQNPTVTYNSAGTYTVTLTATNSLGSNTITQTNYITVINSTGAALPLTEGF
                     QSTTFPPTGWTLATHSGFNWERTTVAGGFGSSTASALFNNYDNDATGLADDMISPSLS
                     ISNVTNPKVKFDYAYAYYSGTQGTAYDTLQVLIWDVCANTYTSIFKKGGTALATAPAT
                     SSSFVPTATQWKSDSVAIPTTFLNKNVKVVFRNISDYGQNLYVDNINIYGTTASSIAA
                     SFTSSADSVCQGGSLTFTSTSTGGPFDSIRWIFPNGTPATSTATNPSVTFNTAATITV
                     TLKVYKSGNSSTSTKTIVVKAKPTVVANGMAICNGNTASITATGATTYSWNTGATGAT
                     LSVSPTTTTTYTVTGTTSGCTNTDTAIVTVNAKPTVIANGSTICAGNSATITASGAGT
                     YTWNTGATGATLTVSPTVTTTYTVTGTGTNGCINTDTAIISVNALPNVSVSSITICSG
                     QTGTLTATGATAYSWNTGATTASISVSPTTTTTYTVTGTANGCTKNATATVSVATAPN
                     VV"
     gene            complement(2817..3401)
                     /gene="hisH"
                     /locus_tag="PLF48_07380"
     CDS             complement(2817..3401)
                     /gene="hisH"
                     /locus_tag="PLF48_07380"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNF49181.1"
                     /translation="MKIVIINYNAGNTKSVENALLRLNVTPLITHDAEEITSANKVIF
                     PGVGHALPTMQCLKQNGLDRVIKQLKQPVLGICLGQQLMCKHSEEGDVECLGIFDVAV
                     KKFNSEKEKIPHMGWNGIKKLNSPLFNNLNEQDDVYFVHSYYCTLSPDTIAETDYILP
                     FSSALHVDNFYAVQFHPEKSASVGNTILENFLML"
     gene            complement(3398..4171)
                     /gene="trpA"
                     /locus_tag="PLF48_07385"
     CDS             complement(3398..4171)
                     /gene="trpA"
                     /locus_tag="PLF48_07385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNF49182.1"
                     /translation="MNRISKLFEIKNENILSIFCTAGFPELNSLPQILIELQHAGVDM
                     VEIGIPFSDPIADGPTIQQANKIALDNGMTIKFLFEQLKDVRKYIDIPLILMGYFNPV
                     MQYGVEHFLSDAKTVGIDGVIIPDLPLYEFETYYKSILEQFDIKNIFLITPQTSKERV
                     QKIDEVSSAFLYLVSSNAITGSTGTLAKNEEYYERIRTLQLKNPSLIGFGIHDKSTFD
                     YACSHANGAIIGSAFIKALQQEKSLQDTIQEFISSLKSR"
     gene            complement(4205..5614)
                     /locus_tag="PLF48_07390"
     CDS             complement(4205..5614)
                     /locus_tag="PLF48_07390"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNF49183.1"
                     /translation="MKQINTFDIGILQDWDAIETAKKIKDKSIHAEEAVSCAVERAER
                     ADVFLNAIVNANYKKALEEAKVNHKGIFAGVPTFIKDLNDVEGFPTLKGSAAFKNKPA
                     KKNDKIVNQILDITGAVILGKTSTSEFGLLPCGETLQHGETRNPWNIAYSTGGSSAGA
                     AALVASGVVPFAHASDGGGSIRIPASCCGLIGLKPTRGRNCTSTTEKAPVDVAQDGIL
                     ARSVRDVALYYSKLTAYSQNKKLPEIGLVTHPGKKRLKIGMFTQSSLGITAQADVENT
                     VEATGKLLEDMGHQVSYISNPFEHKVTRDFLMYWSFLSFASMITEYAANGLDYRHSKT
                     AKFTKQLAAAFPFLSIASFSSIRNLQKHIQDYNTILEDFDVLLSPTLSHPAPPIGHFG
                     TEVDALEIVMKLNAYVNFTTTQNITGAPAISLPMGISSDGLPIGVQLATGVGDEHTLL
                     ELAYELEETNSFKMLHLLK"
     gene            5874..>6605
                     /locus_tag="PLF48_07395"
     CDS             5874..>6605
                     /locus_tag="PLF48_07395"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789678.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNF49184.1"
                     /translation="MQYDEFIEKYASHPTYKAPRGNTLHAKSWQTEAPLRMLLNNLDA
                     EVAENPAELVVYGGIGQAARNRTALENIVRALLELDENHSLLVQSGKPVGIVRTHPQA
                     PRVLIANSNLVPKWATWEHFYELRDKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEC
                     GRQHFNGNLHGKLLVSSGIGGMGGAQPLAAVMAGATYLGADINKASIQKRLDTKYIDF
                     MFETYEEARDKALDSKAKGEAISIGV"
BASE COUNT         1931 a         1310 c         1259 g         2105 t
ORIGIN      
        1 ggacaacatt tggtgcagtt gctactgata ccgttgccgt tgcattctta gtgcaaccat
       61 tagctgttcc tgttactgta taagttgtcg tagtagttgg tgaaacagaa atacttgcag
      121 tagtagctcc ggtattccaa gaataagcag tagctcccgt tgccgtcaaa gttcccgttt
      181 gtccactaca aatagtgata gatgaaacac ttacattcgg taaagcattt acactgatta
      241 ttgcagtatc tgtatttata catccgtttg tgcctgtacc tgttaccgta taggtagttg
      301 tcactgtagg agaaacagtc aatgttgctc ctgttgctcc cgtattccat gtataagttc
      361 cagctccgct tgctgttatt gtagctgaat tacctgcaca tattgtgctg ccattagcaa
      421 taacggtagg cttagcattt acggttacta ttgcagtatc tgtatttgta catccacttg
      481 ttgtgcctgt tacagtataa gtggtggtgg tggtgggtga aacagataat gtagctcccg
      541 tagcccctgt attccaagaa taagtagtag caccggttgc agtaatagat gctgtattac
      601 cattacagat ggccattcca ttagccacta ccgttggttt agcttttaca acaatagttt
      661 ttgttgatgt actgctatta cctgatttat aaacttttaa agtaacagta atcgttgctg
      721 cagtattaaa agtaacactt ggattcgtag ccgtagatgt tgcaggtgta ccattaggga
      781 atatccatct gattgaatca aaaggaccac cagtagaagt gctggtaaac gtcaggcttc
      841 caccctgaca aacggagtct gcagatgaag taaacgatgc ggctatggat gatgcagtag
      901 tgccataaat attaatatta tccacataca ggttttgtcc gtaatcgctt atgtttctga
      961 aaacaacctt tacattttta tttaagaaag tagttggaat agcaacactg tcagatttcc
     1021 actgtgttgc tgttggaacg aaactgctgc ttgtagccgg agctgtagcc aatgcagtgc
     1081 cccccttttt gaaaatactg gtataagtat tcgcacaaac atcccaaatt aatacctgta
     1141 atgtatcata agctgttcct tgtgttccgc tataatatgc atacgcataa tcaaatttca
     1201 ctttaggatt agttacatta ctgatgctta atgaaggtga aatcatatca tctgctaaac
     1261 ctgtcgcatc attgtcataa ttattaaata gagctgatgc agtcgatgaa ccaaatcctc
     1321 cggctacagt tgttctttcc cagttaaatc ctgaatgggt agctaatgtc catcctgttg
     1381 gaggaaatgt agtgctttga aatccttcag tcagtggcaa agctgcccca gtagaattta
     1441 taacagtaat gtagtttgtc tgtgtaatgg tgttagaacc taatgaattg gtagccgtta
     1501 aagtaacggt gtaggttcct gctgaattat acgttactgt tggattttgt aaagtagaag
     1561 tagctggtgt tcctccggcg aatgtccaag accaagaagt gggcgtattt gaggaattat
     1621 ctgtaaatgc aactgtacca cccacgcaga ctgtcgtttt attagaggta aaattagcta
     1681 cgggcgctga aggacctgca ttacacgtag atgcactgaa aaacttacgg taagtgccgt
     1741 tcgctaatgc ggtttgcata cgggtagtct ggttaggtgt aaacatatac atacaagcat
     1801 cgtccgtata atccataaag ttcataaaca tgtctccgtt tgggccatta ctacaagtaa
     1861 cacttggata agtaggacag ccataattta atccttgacc atctccagca ttacctgact
     1921 gaacgggagt atctccacaa tagtcatttc cacagttact atcgccacta atatggcgca
     1981 gacctagcca gtgtcctatt tcatgagagg cagttctgcc tttgctgtat gcacccgtac
     2041 ctactgtacc aaaattagtg taattaatta ctacaccatc atctgtagct gtaccaaatc
     2101 ctgaaagccc gctcaagcca gtacctgcgg gaaatgttgc gtagcctaat attcctccgc
     2161 ttattggcaa tacccatata tttaaatatt tagttggatc ccaaatggta tttggtttaa
     2221 tattagcatc aatataggta tctgtccatc ccgaagtccc cggattagtg aacccctttg
     2281 taactgcatt tactctgtca attccgggct ctaacagcgt aactccattt ggatcttttg
     2341 cagccaaaca aaatgtaata ttggtatttg ccactaaaga ttgaaatact gcaggacaat
     2401 tacctacatt taaacctgtt cccgcatagt ctgcatttaa agtattaatt tgagattgta
     2461 cttggctctg aggaatattt tgagcagaag tataatatac aacatgtaca attacaggaa
     2521 tagtataagc aactgatatt ctgcttgcag gattgctatg tgctgcctga aactgttgaa
     2581 tttgctgctg aaatgcgttt tcccattctt cacccggcat tttggtaata caacgttgta
     2641 tgccattatg tacttgtgag acggaagcgg gacgaggagt ttttgttccg gaaaataagg
     2701 caaaacccat aaccatgaat cctaaaagaa ggagtacatt ttttctcata aaaaataaat
     2761 tgttgagcga atttactaag aaataattag aaaaccacct tttctgatta gaaaaatcat
     2821 aacattaaga agttctctaa tatcgtattt cctacagatg cgcttttttc aggatgaaat
     2881 tgaacggcat agaaattatc tacatgcaat gcagatgaaa aaggcaaaat atagtcggtt
     2941 tctgcaatcg tatctggcga taatgtacaa tagtaagaat gtacaaaata gacatcatcc
     3001 tgctcattca aattattaaa aagcggagaa tttaatttct ttatcccgtt ccatcccatg
     3061 tgcggaattt tctccttttc ggaattaaat tttttcacag ctacatcaaa aatacccaga
     3121 cattccacat ctccttcttc gctatgcttg cacatgagtt gttgtcccaa acagatacct
     3181 aatacaggtt gctttagttg ttttataact cggtctaatc cgttttgttt taagcattgc
     3241 atcgttggca aggcgtgccc cacaccggga aaaataactt tattcgcact cgttatttct
     3301 tctgcatcat gtgtaatgag tggcgttaca tttaacctca ataatgcatt ctccacactt
     3361 ttagtattgc cggcattata atttatgatg actattttca tcggcttttc aaagatgata
     3421 taaattcttg aatagtatct tgaagagatt tttcttgttg taaggcttta ataaatgcac
     3481 ttccgataat tgctccattt gcgtgtgaac aggcataatc aaacgtggac ttatcatgaa
     3541 taccaaatcc aatcaaagac gggtttttta gctgtaaagt ccggatacgc tcatagtatt
     3601 cttcattttt agccagtgtg ccagtgcttc ccgttatcgc atttgatgaa accaaataaa
     3661 gaaatgcgga actgacttca tcaatttttt gaaccctttc cttacttgtt tgtggtgtta
     3721 tcagaaaaat atttttgata tcaaattgct ctaatatgct tttataataa gtttcaaatt
     3781 catacaaggg caaatccgga ataatgacac catcaatacc taccgttttt gcatcactca
     3841 aaaaatgctc aacaccatat tgcattacgg gattaaagta gcccattaaa atcaatggta
     3901 tatcaatata tttacgcaca tctttaagtt gctcaaataa aaatttaatc gtcattccat
     3961 tatctaaagc aattttgtta gcttgctgaa tggtggggcc atctgctatt gggtccgaaa
     4021 acggtatccc gatttccacc atatccactc ctgcatgttg caattcgatt aagatttgcg
     4081 gcaaggagtt caattcagga aatcctgctg tacaaaatat actgagtatg ttttcgtttt
     4141 ttatttcaaa aagcttggaa attctgttca tctataatgt aaatttttgt aaatatatta
     4201 tattctattt taataaatga agcattttaa agctgttcgt ttcttccagt tcataagcaa
     4261 gctccaaaag tgtatgctca tctcctactc ctgtagccag ttgcacacct ataggcaagc
     4321 cgtcgctgct tatgcccatt ggcagtgaaa tggcaggtgc tcccgtgata ttttgtgtgg
     4381 tagtaaaatt gacatacgca tttagtttca tcactatttc caatgcatct acttctgtgc
     4441 caaaatgccc aataggtgga gccggatgtg ataatgtagg gcttaataat acatcaaaat
     4501 cttctaaaat agtattataa tcttggatat gcttttgcaa atttcgaata ctgctaaatg
     4561 atgcaatgga taaaaatgga aaagctgctg ctaattgttt ggtgaatttt gctgttttag
     4621 aatgcctgta atccaagcca ttggcagcat actccgtaat catggatgca aaggataaaa
     4681 aagaccagta cattaaaaaa tcgcgggtta ctttatgctc aaatggatta gaaatatagc
     4741 ttacctgatg ccccatatct tccaagagtt ttcctgttgc ttctaccgta ttttctacat
     4801 cagcttgtgc ggttatacct aatgatgatt gggtgaacat gccaattttc aatctttttt
     4861 taccgggatg cgtaacaagc ccgatttcag gcaatttttt attctgcgaa taagctgtca
     4921 gctttgaata atacaatgcg acatctcgta cacttcgagc taatataccg tcttgagcaa
     4981 cgtcaacagg tgctttttct gttgtcgaag tacaatttct gcctcttgta ggttttaatc
     5041 cgattaaccc acaacacgat gccggaatgc ggatagagcc gcccccgtca gatgcatgtg
     5101 caaaaggcac cacacctgat gccactaagg cagcagcccc ggctgatgaa cctcctgttg
     5161 aataagcaat attccacgga tttctggttt cgccatgttg taaggtttcg ccacagggca
     5221 ataagccgaa ttctgaggtg gatgtttttc ccaaaataac agctccggtt atatctaaaa
     5281 tctgattgac tattttatca ttttttttag ccggtttatt tttaaaagcg gcagagcctt
     5341 tcagcgtagg aaatccttcc acgtcattta aatctttaat aaaagtggga actccggcaa
     5401 atatcccttt gtggttgact tttgcttcct ccagtgcctt tttatagttt gcattgacaa
     5461 tagcatttaa aaaaacatcc gcacgttctg cacgctctac cgcacagctt actgcttctt
     5521 ccgcatggat agatttgtct ttaatttttt ttgcagtttc aatagcatcc caatcttgta
     5581 aaataccaat atcaaatgta tttatttgtt tcatgtcagc agtattgtat gtctaatttc
     5641 taaaacctga gttcgggata agcgtttcaa tttaaaaaat agttgatttt ttatcaagaa
     5701 ccacctcctt ttaattcctc ctccttatta agttggagga aagtgtgaga aatcaactat
     5761 tttatcagct tatttttctt aacctgaact taagttaaaa atacgctttc atattttaat
     5821 tcttccttcc atcaaattaa ttttcattta tctttgaata aaaaaacaac tacatgcaat
     5881 acgacgaatt tattgaaaaa tacgcttccc atcccactta caaagctccg cgcggcaata
     5941 cattacatgc aaaatcatgg caaacagaag cccccttgcg catgctctta aataatttag
     6001 atgcagaagt agcagaaaat cctgccgaat tagtcgtgta tggtggcatt ggtcaggcag
     6061 cccgcaatcg cactgcttta gaaaacatcg taagagcctt attggaatta gatgaaaatc
     6121 actctctttt agtacaaagc ggcaagcctg taggcatcgt gcgtactcat ccgcaagcac
     6181 cacgtgtact gattgccaat tccaatttag tgccgaaatg ggcaacatgg gagcattttt
     6241 atgaactgcg tgataagggc ttgatgatgt acgggcaaat gaccgcaggc tcctggattt
     6301 atatcggctc gcagggaata gtacaaggca cgtatgaaac ttttgtggaa tgcgggcgac
     6361 aacattttaa cggcaacttg catgggaaac ttttagtaag ttccggaatt ggcggtatgg
     6421 gtggcgcaca gcctttggca gcagtgatgg caggtgctac ctatttaggt gctgatatca
     6481 ataaggcgag tattcaaaaa agattagaca cgaaatatat tgattttatg ttcgaaacct
     6541 atgaagaagc ccgagataaa gcccttgatt cgaaagcaaa aggcgaggct atttctatag
     6601 gtgtg
//
LOCUS       DAOQII010000133         6549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6580_length_6549_cov_5.807515, whole genome shotgun sequence.
ACCESSION   DAOQII010000133 DAOQII010000000
VERSION     DAOQII010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6580_length_6549_cov_5.807515"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..676
                     /locus_tag="PLF48_07400"
     CDS             17..676
                     /locus_tag="PLF48_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49185.1"
                     /translation="MKKTKLVVALMLIFAYATSTAGELGLNMETMNMYAPTAKGKSKS
                     SGGKAFEGKGSKTLSIGLGMSSYLGAFGKGGFYRYGGRGYYYGAFWYSGTLPIQLEFG
                     VHDYVGVGLVTGFGGRAYAGGGGVLYVPFGVVANFHFMQLIGDKKGVAIADKLDVYAG
                     LNVGTGLAVSFPTGTGSVGGMLFAGPQIGLKYFFNEKIGVYTEFGYGKSLIEGGVSIK
                     L"
     gene            complement(750..1190)
                     /locus_tag="PLF48_07405"
     CDS             complement(750..1190)
                     /locus_tag="PLF48_07405"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNF49186.1"
                     /translation="MKKLFLLFALVCLAKAKIFAGGPDDVLGTWLTGEKDYKIQLTKN
                     AAGEIEGHVVWMAEPNDKNGKPRTDVDNDDPALRSRPVMGLKVVWGFKYVAANKDYED
                     GKVYKKGNIYCGKMQLNPDGTLKLTGFICKVSFLKKKDTWTRVK"
     gene            complement(1301..1723)
                     /locus_tag="PLF48_07410"
     CDS             complement(1301..1723)
                     /locus_tag="PLF48_07410"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNF49187.1"
                     /translation="MINYEELFMKEALRLAVKAFEEDEVPVGAVVVYGQQIIGKGYNQ
                     VEKLNDPTAHAEILAITAACNYLGSKYLDKCTLYVTLEPCMMCTGAIREAQIQKVVFG
                     AGDNSHRKSQPNQFELQGGILEDECSSLLKRFFKLKRL"
     gene            1810..3243
                     /locus_tag="PLF48_07415"
     CDS             1810..3243
                     /locus_tag="PLF48_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF49188.1"
                     /translation="MSGNHLKILYFFLFLSVLQIHPVHSQETWDWKKCIDYALQNNLQ
                     LKQSDLNIDLQQVNLKQYKYNFTPNISASTNYNLRFGNNYDFYTSQYKKQAINYQDYS
                     LNIVQPVFDGLAYKHNIEKTNIDIQTLKLDEEVLKTNIQLQILMAFLNIMNAQEQLTQ
                     AQNQYAQSQEQYQQVKDMIEAGTSAEKNLVDVEAQLTNEDLAISQVNNQLMLAYLNLK
                     QIMQLDLSKDIKIKTPQLPDDIVIAAPAALGNIYQAALGIRPEIKSAELKIESSKKAV
                     DIAKTSFYPTLKFVSNINTFMSTQNKNTQQYLTGNTTAVGFVEGTFQRVLIPESGYKQ
                     SKIPYFKQLNQNLNYALGLSLDIPIFSQYRVQTSIKQAQLQTKVASFQKQMEEQTLYN
                     TIAQAYLKTLASIDNYHAAKKNFETAQKSYDFAMERWDAGMINLLETNIAKTNLLNAE
                     SKFIQTKYEYLFNSKVLDFYQGKKIEL"
     gene            3257..4567
                     /locus_tag="PLF48_07420"
     CDS             3257..4567
                     /locus_tag="PLF48_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF49189.1"
                     /translation="MKSNKLLYTLIGLTIAAVIAGIIFGTNKNKNKAMEVELEKISTR
                     NIVQTVTASGKIYPEEEVKISSDVSGEIIELTVKEGDVVQKGKLLARIKPESYQANVE
                     QTQANINNMQSQLSTAKSRIAQAQAQYNNALNTFNRTKELYNKKIISKAEYQNAETAL
                     NSAKADVEAAKHNAEAAGYSMKASQAIKKEANINLSKTTIYAPMNGIISLLNVKQGEK
                     VVGTLQMSGTEMMRIANFEYMEVRVDVSESEIIKVKVGDTAEVEVDAYLDRKFLGIVT
                     EVANTSKGASASSVLSTDQSTNFVVKIRILLSSYQDLLTETAKPFLPGMSATVSIRTK
                     TKEGVIAVPIQAVTTKDTVINGTTKTLELVYVNDNGKAKIRYIKTGIQNNEYIEVLEG
                     LKGNETIIKGPYNVISKELKENDAVKEKVEKEKGKDKKEPEDKE"
     gene            4617..5390
                     /locus_tag="PLF48_07425"
     CDS             4617..5390
                     /locus_tag="PLF48_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49190.1"
                     /translation="MYKIFLIGVLSCCAISFSQAQDTISSDTNFEFLLPFKTADAGNT
                     SVYYTQAQQYFANKNYEPALALIKKAIKQDKANADFYLLKAWILSAQGNTKSALQTAE
                     KAVNLKPESWKSLYCVAYCKQVASDYLGAVIAYTQVLQKKPDHSLSLSSRAKCKEWLK
                     EYSSAIEDYSAAIRLLSTEPKLYAARANLYFKTQQYDAAIADYSTCMLYDKSNAEACY
                     YRGMCYVSKNDINNACIDFAKANTLGFKDAKSAFDKYCR"
     gene            complement(5396..5617)
                     /locus_tag="PLF48_07430"
     CDS             complement(5396..5617)
                     /locus_tag="PLF48_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49191.1"
                     /translation="MSDNEITLIKEITKITLQIRSEYPEVYSKLEEMPITVPNSTAGS
                     VRIKDLQDYLDSLYQIKKEFDSNRNIETE"
     gene            complement(5696..6340)
                     /locus_tag="PLF48_07435"
     CDS             complement(5696..6340)
                     /locus_tag="PLF48_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49192.1"
                     /translation="MKVILISIFLGISSYLIAQDLTEVNSSEKSVKEKKFIFRAGTMS
                     GLNFYTNPNANEVSYKRVSIGSGLGAELGYRVHKLLVIEANYYYSIFKNKVSPDNVSV
                     LFSKLKLKQQHQMVSFTLNIDIYKNAKTRIYAGTGAGFSLNAEQFIRYIPGTNTAAEI
                     GERNCFKFITPVHFGMDSYIKDKVVLGTRVNTFIWGDKKPFRSLNLFMSLGMRF"
BASE COUNT         2244 a         1178 c         1197 g         1930 t
ORIGIN      
        1 gaaaaaaatt acaattatga aaaaaacaaa attagtagtg gcgttgatgc tgatattcgc
       61 atacgctaca tccacagcgg gagaattagg tttgaacatg gaaactatga acatgtatgc
      121 tcccacagct aaaggcaaaa gtaaatcttc cggcggtaaa gcatttgaag gtaaaggttc
      181 aaaaacctta tctataggct tgggcatgtc ttcttattta ggagcattcg gcaaaggagg
      241 tttttacaga tacggaggaa gaggttacta ttatggagca ttttggtatt cgggaacttt
      301 acccattcaa ttagagtttg gtgttcatga ttatgtagga gtaggtctgg taacgggatt
      361 cggtggaaga gcatatgctg gcggtggcgg cgtattatat gttccatttg gtgtagtggc
      421 taactttcac tttatgcagc taattggtga taaaaaagga gtggctattg cagataaatt
      481 agacgtatat gcaggcttaa atgttggtac tggtcttgcg gtaagttttc caaccggcac
      541 tggtagcgta ggaggtatgt tgtttgcagg tcctcaaatt ggtcttaaat acttcttcaa
      601 tgaaaaaatc ggtgtttaca cagaattcgg ttatggcaaa agcttaatcg aaggtggtgt
      661 ttccatcaaa ctgtaaattc aaaaaatata aactacaaaa gaaaaacagc gccattttgg
      721 cgctgttttt tttattgata taatttcttt tactttactc ttgtccaggt gtctttcttt
      781 tttaagaatg aaactttgca aataaagcct gtcaatttca atgttccatc agggttcagt
      841 tgcattttac cacagtaaat attccctttt ttgtaaactt tgccgtcttc gtaatcctta
      901 ttagccgcca catatttaaa tccccaaact acttttaatc ccattaccgg gcgagaacgc
      961 aacgccggat catcattgtc aacatccgta cggggctttc cgtttttgtc attaggttct
     1021 gccatccata cgacatgacc ttcaatttct ccggccgcat ttttagttaa ttgaatcttg
     1081 tagtcttttt ctccggtaag ccaggtgcct aaaacatcat ccggcccacc tgcaaaaatt
     1141 ttagccttgg caaggcatac caaagcaaac aatagaaata gttttttcat gctgattatt
     1201 ttttttgaag ttaataaaag tttacgaaaa ttaaataaca aaataaatgc caataattca
     1261 gtcatttacg cttttaaaac atacagtgat atcgctgatt ttataatcgt ttcaatttaa
     1321 aaaaacgctt tagcaatgag ctgcactcat cttctaaaat acccccttgc agctcaaact
     1381 gattgggttg tgatttccta tgtgaattgt cgccggctcc aaaaactact ttttgtatct
     1441 gcgcttcccg aatggcaccc gtacacatca tacagggttc aagtgttaca tagagcgtac
     1501 atttgtcaag atatttgctg cccagatagt tgcaggcagc agtaatcgcc agaatttcag
     1561 catgtgcagt cgggtcattt aatttctcta cctgattata tccttttcca ataatttgct
     1621 gtccatatac gacaaccgca cctaccggaa cctcatcttc ttcaaaagct ttgacagcca
     1681 gccgtaaggc ttctttcata aataattctt cataattaat cataattaca aatgcgtgta
     1741 tttgatgata gatttagaaa atgtactttt acgctgtaat atatgcataa aatctgcaac
     1801 ccatatacca tgtccggaaa tcatctgaaa atcttgtatt tttttctttt cttgtcagta
     1861 ctgcaaattc atcctgtaca ttcgcaagaa acatgggatt ggaaaaaatg tattgattat
     1921 gctttgcaaa ataatttaca attaaaacaa agtgatttaa atattgacct tcagcaagtt
     1981 aatctaaagc aatataaata caactttact ccaaacatca gcgcatctac caattataac
     2041 ttgcgcttcg gcaataacta tgatttttat acatcacaat ataaaaaaca agcaatcaac
     2101 tatcaggatt atagtttaaa tattgttcaa cctgtttttg acggattagc ctacaagcat
     2161 aatatagaaa aaacgaatat cgatatacaa acactaaaac tggatgagga agtattaaaa
     2221 accaatatcc agcttcagat actgatggca ttcctcaata ttatgaatgc acaggagcaa
     2281 ttaacacaag ctcaaaatca atatgcacag tcgcaagagc aatatcagca agtaaaagac
     2341 atgatagaag caggtacttc tgcagaaaaa aatttagtag atgttgaggc tcagctgacg
     2401 aatgaagatt tggctatttc gcaagtaaac aatcaactaa tgcttgctta tctcaatctt
     2461 aaacaaatta tgcaactcga tttatcaaaa gatattaaga taaaaacgcc tcagcttcca
     2521 gatgatattg tgattgctgc acctgctgcc ttaggaaata tttatcaagc agctttgggt
     2581 atccgtccgg aaataaaaag tgccgaactg aaaatcgagt catcgaaaaa ggctgtagat
     2641 atagcaaaaa cgtcctttta ccccactttg aaatttgttt ccaatatcaa tacatttatg
     2701 tctactcaaa ataaaaatac acaacaatat cttaccggaa atacgacggc tgtcggtttt
     2761 gtagaaggca catttcagcg tgtgttgatt ccggaatccg gttataaaca atccaaaatt
     2821 ccttatttca aacaattgaa tcaaaacctg aactatgcat tgggcttaag tcttgatatt
     2881 cctatattca gtcagtaccg cgtgcaaact tctattaagc aagcacaatt acaaacaaaa
     2941 gttgcaagct tccaaaaaca aatggaggaa caaaccttgt ataataccat tgctcaggca
     3001 tatctcaaaa cgctggcatc tattgacaac taccatgcgg ctaaaaagaa ttttgaaacg
     3061 gcacaaaaat cttatgattt tgccatggaa cgctgggatg ccggtatgat caatttgctg
     3121 gaaactaata tagccaagac gaacttattg aatgcagaat ctaaatttat acaaacgaaa
     3181 tacgagtatt tatttaacag caaagtgctg gatttttatc aaggtaaaaa aatagaattg
     3241 taataaacag aataaaatga aaagcaacaa actattatat acactcatcg gattgacaat
     3301 tgctgctgtc atagccggaa ttatttttgg tacaaacaaa aacaaaaata aagcaatgga
     3361 agtagagctg gaaaaaatca gtaccagaaa cattgtacaa acagtaacag caagcggcaa
     3421 aatttatccg gaagaagaag tgaaaatcag ttcggatgta tcaggcgaaa ttatagagct
     3481 gactgtaaaa gaaggcgatg tagtacaaaa gggtaaatta cttgcccgta taaaaccgga
     3541 aagctatcag gctaatgtag aacagacaca agccaatatc aataatatgc agtcgcaatt
     3601 atcaacggca aaatcgcgta ttgcacaggc acaagcacaa tataataatg ctttgaatac
     3661 tttcaaccgg acaaaagaac tgtacaataa aaaaatcatt tcaaaagcgg aatatcaaaa
     3721 tgcggaaaca gcattaaact cggcaaaagc agatgtagag gctgcaaaac ataatgcaga
     3781 ggcagcaggg tattccatga aagcatcaca agccataaaa aaagaggcaa atattaattt
     3841 gagtaagacc acgatttatg caccgatgaa tggcataatt tctttgctga atgtaaaaca
     3901 aggcgagaaa gtagtaggta ctttacaaat gagcggaacg gaaatgatgc gcattgctaa
     3961 cttcgaatat atggaagtgc gtgtagatgt aagcgagagt gagataataa aagtaaaagt
     4021 aggcgatact gccgaagtag aagtagatgc ttatttagac cgcaaatttt tgggcatagt
     4081 tacagaagtt gccaatacct ctaaaggtgc aagtgcaagc agtgttttaa gtacagacca
     4141 gtccaccaat ttcgtggtga aaatcaggat tttattatcc tcctaccagg atttactgac
     4201 agaaacagcc aagccgtttt tgcccggtat gtcggctaca gtaagcatca gaacgaaaac
     4261 aaaagaaggc gtgatagcag tacccattca ggctgtaact acgaaagata ctgttatcaa
     4321 tggtacaact aaaaccttgg aactggttta tgtcaacgac aatggaaaag ccaagatccg
     4381 atatataaaa accggcattc agaataatga atatatcgaa gtgctggaag gtttgaaagg
     4441 aaatgaaacc attattaaag gtccgtataa cgtaatatcc aaagagctga aagaaaatga
     4501 tgccgtaaaa gaaaaagtag aaaaggaaaa aggcaaggac aaaaaagaac cagaagataa
     4561 agagtaattg gtaaattagg cattattctc aaaaactaaa ttcaatatta acgtctttgt
     4621 ataaaatatt tttgatagga gttttatcgt gttgtgcaat atctttttca caagctcagg
     4681 acaccatttc ctcagatact aattttgaat ttttactgcc ttttaaaaca gctgatgccg
     4741 gtaacacttc cgtttattat acgcaagcac aacaatattt tgccaataaa aattatgaac
     4801 ccgctctcgc acttatcaaa aaagcgataa aacaagataa agcgaatgct gatttttact
     4861 tactgaaagc atggatatta tcggcacaag gcaatacaaa gagtgctttg caaactgcag
     4921 aaaaagcagt aaacctcaag cccgagagtt ggaagtcttt gtactgtgtt gcctattgca
     4981 aacaggtcgc ctcagattat ttgggcgcag ttattgccta tactcaggta ttacaaaaaa
     5041 aacccgatca tagtttgtcg ctgtccagcc gtgcgaagtg caaagagtgg ctcaaagagt
     5101 atagcagtgc aatagaagat tattcggcag cgataagact gcttagtaca gaaccaaaac
     5161 tatatgcagc tcgtgcaaat ttatacttca agacacaaca gtatgatgca gctatcgcag
     5221 actattcaac ttgcatgctt tatgataaat caaatgcaga agcctgctac tacagaggta
     5281 tgtgttatgt ttcaaaaaat gacatcaata atgcttgcat agattttgca aaagcaaata
     5341 ccttaggttt taaggatgcc aaatctgctt ttgataagta ttgcaggtaa aaatattatt
     5401 cagtttcaat attgcggttg ctatcgaatt ctttcttgat ttgataaaga gaatctaaat
     5461 aatcttgtaa gtctttaatc cttactgaac ccgcggtgct gtttggtact gttattggca
     5521 tttcttccag tttggaatat acctccgggt actcgctcct gatttgcagg gttattttcg
     5581 tgatttcttt tattagtgta atctcgttgt cagacataat atcggttttt tctaaaaata
     5641 aggattttta agcaaaaatg caagtaaaaa aggttcaaat tgtgcctgat tgccgttaaa
     5701 agcgcatgcc taaagacata aacaagttta gacttctgaa tggtttttta tcgccccaga
     5761 taaatgtatt caccctggtg cctaaaacca ctttgtcttt gatataactg tccattccaa
     5821 aatgaacagg agtaataaat ttgaaacagt tgcgttcgcc aatttcggca gccgtattag
     5881 tgccgggtat gtatcgtata aactgttcgg catttaagga aaatccggct cctgtgccgg
     5941 cataaatcct ggttttggca tttttataaa tgtcaatatt caatgtgaaa gaaaccatct
     6001 gatgttgttg tttcagtttt aatttgctaa ataatacact tacattatca gggcttacct
     6061 tattcttaaa tatagaataa taatagttag cttcaataac gaggagcttg tgtaccctgt
     6121 agcccagctc ggcacccagc ccgctaccga tggacacgcg tttgtaacta acttcattag
     6181 cattcgggtt cgtgtagaaa tttaagcccg acatggtacc cgcccggaaa ataaatttct
     6241 tttctttaac agacttttct gatgagttga cttcggttaa atcctgggcg attaagtatg
     6301 aacttatacc aaggaaaatg gaaatcaaaa ttactttcat tcgataaaag tattgaattt
     6361 agacgatttt tttaaaccta ttttttttaa tgatcatgca agattaagaa agtattttcg
     6421 ttatacaaat gtgagactaa aatttatcat atttaatatg tatctaacta ttttgctggc
     6481 attacattcc gtctgtttcc aaatccaaca aaatagctta ttgcagcaat aagattccca
     6541 tttttagta
//
LOCUS       DAOQII010000134         6522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6618_length_6522_cov_5.563631, whole genome shotgun sequence.
ACCESSION   DAOQII010000134 DAOQII010000000
VERSION     DAOQII010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_6522_cov_5.563631"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..2293)
                     /gene="recQ"
                     /locus_tag="PLF48_07440"
     CDS             complement(80..2293)
                     /gene="recQ"
                     /locus_tag="PLF48_07440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNF49193.1"
                     /translation="MSVKNETMLSALKEYFGFEKFKGNQEAIIESIISKKDTFVIMPT
                     GGGKSLCYQLPAVMNEGTAIIISPLIALMKNQVDLIRGYSNVDNIAHFINSQQNKTQT
                     NQVKKDIVDGKCKLLYVAPESLVKQENIDFLKTIKISFVAVDEAHCISEWGHDFRPEY
                     RKIRQMLNDIEGNIPIVALTATATPKVQQDIVKTLGMHDPQIFISSFMRENLFYEVRP
                     KPSKQDAIKDIIKYIKKEGNKSGIIYCLSRNTTEELAEVLSVNGVNAMAYHAGLDAHT
                     RSERQDQFLMEDIQVIVATIAFGMGIDKPDVRFVIHFDIPKSLENYYQETGRAGRDGL
                     QGVCIAYYSPKEIHKFEKFILSNKEKTVAEKEIAVMQLSEVEAYAETGECRKKFVLHY
                     FGEETGNCGMCDNCRNPKEKVEAKEEMKLALQVVAETNENVNIVNTINILTGRKSADI
                     TTNNFDKLKIFGKGADKDMLYWNSVVRKAILLNYLGKDIEQYGKLFLTDEGKKFIKKA
                     SSVQIVLNNKFADGEDDDDDTVVMGSKSGSVLEPNLFKILKEIVYTEGKKHKIPPYTV
                     FQDPSLEEMATLFPITVDELAQINGVNKAKAERFGKPFLQAISAYIEENEIERTADFS
                     NIKSADKNSNIKVNIIKSIDKKMPLEHIAKSFNISMEDLIGELDSIVNSGTRVNIKYY
                     IDDVIDEDIQEEIFDYFMEADTDSVDTAYKKLKEEDIEYREIQLVRIRFLSEVAN"
     gene            2498..3580
                     /locus_tag="PLF48_07445"
     CDS             2498..3580
                     /locus_tag="PLF48_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNF49194.1"
                     /translation="MNANAYVAIMAGGIGSRFWPASRTGKPKQFLDILGTGRTLIQAT
                     YDRFTKICPKENIFILTHSDYIPLIKEQLPDVTDVQILAEPARKNTAPCIAYASMKIR
                     KINRDGNMIVAPSDHIIMNEDEFVRICNTALDFVSQHNALATLGIRPTRPDTGYGYIQ
                     YMEKEVAPEIHQVKTFTEKPQKEVAQQFIKSGDFLWNAGIFIWNVNTVISALESNLNE
                     VYEAFAEIESDLLTAKEQEGVVTAFTQCPSISIDYGIMEKAKNVYTIPSSFGWSDLGT
                     WASLYVEKEKDYLQNAVNGNNVVIYDSTHNIINVPENKLVVIQGLENFIIVDTDDVLL
                     ICNKDEEQRIKEFTHDIKMNKGEKFL"
     gene            4158..5180
                     /gene="hemH"
                     /locus_tag="PLF48_07450"
     CDS             4158..5180
                     /gene="hemH"
                     /locus_tag="PLF48_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNF49195.1"
                     /translation="MLQKKTGVLLVNLGTPDSPGNKDVYKYLIEFLTDERVIDFGWLK
                     RNLLVRGIIVPARYKHSASTYRKIWDSERGSPLLYHTQDLGKLVQQQLGDEFIVEIAM
                     RYQSPSLETALEKMRQLQVNKIVVLPLFPQYSSACTGSILQKVQDITSTWQTIPDIKF
                     IHQFYDDALFINAFATRGKSYNFDEYDHVVFSYHGIPARQIKKADISGCHCLQKEDCC
                     SKIESMNQHCYKAQCYQTTRLLVKELNLPKEKYTLSFQSRLGKEVWLEPYTVDVLPQL
                     AREGKKKLLIFCPAFVADCLETLYEVQEEYLELFRGHGGESITLVESLNSEPEWVDAV
                     CHLVKN"
     gene            5185..6369
                     /gene="mqnE"
                     /locus_tag="PLF48_07455"
     CDS             5185..6369
                     /gene="mqnE"
                     /locus_tag="PLF48_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNF49196.1"
                     /translation="MMVALENIEQILLLPGTDARLKAIAIKVLHGERINMEEGILLYE
                     KATVGFVGALANFIRNKKHGDNTYFNRNFHVEPTNICVYTCKFCSYSRLIKQREQGWE
                     LSVEQMLDIVKKYDNQPVTEVHITGGVIPKQDFEFYQNLFSAIKKHRPGLHIKALTPV
                     EYHYIFKKAKVTYEEGLRAMKAAGLDSMPGGGAEIFDDKIRDEIAGGKCSAQEWLDMH
                     ETAHKLGIPSNATMLYGHIETFAHRVNHMERLRQLQDRTHGFNAFIPLKFRNQHNEMQ
                     HLPEVTVIEDLRNYAIARIYLDNFDHIKSYWAMIGRNTTQLSLNFGVDDIDGTIDDTT
                     KIYSMAGSEEQAPAMSTRELVNLIKAVGRKPIERDTVYHVITDYSDIVFEEDEKEKAR
                     NY"
BASE COUNT         2031 a         1260 c         1203 g         2028 t
ORIGIN      
        1 aaaaactgag aaatcaacta tttttttact attttcttaa cccgaactca cgttactaca
       61 ttttacttac tactttccac taattcgcca cttcactcaa aaaccggatg cgaacaagtt
      121 gtatttcgcg gtattctata tcttcttcct tcagtttttt gtaggcagtg tccacactat
      181 ctgtatcggc ttccataaaa taatcgaaaa tttcttcttg aatatcttca tcaatcacat
      241 catcaatata gtatttgatg tttacccgcg taccggagtt gactatagaa tccagctcgc
      301 cgattaaatc ctccatgctg atattaaagg acttggcgat atgctccaga ggcatttttt
      361 tatcgatgga tttgataata tttactttga tattcgaatt tttatccgcc gatttaatat
      421 tggaaaaatc agcagtacgt tcgatttcat tttcctcaat atatgcacta atggcttgca
      481 ggaatggctt gccgaaacgc tcggctttcg ctttgtttac gccatttatt tgggcaagtt
      541 catccaccgt tatcggaaat aaagtggcca tttcttcgag tgatggatct tgaaaaacag
      601 tataaggcgg tatcttatgt tttttgcctt ctgtatagac aatttctttc agaattttga
      661 acaaattggg ttccaaaacc gaccctgatt tgcttcccat cactaccgtg tcatcatcat
      721 cgtcttcgcc atctgcaaat ttgttattga gtacaatttg cacagatgat gctttcttga
      781 tgaatttctt tccttcgtca gtgagaaata atttgccgta ttgttcaata tctttaccca
      841 gatagtttag caaaatagcc ttgcgcacga ctgaattcca atacaacata tctttatctg
      901 cacccttacc gaatatcttc agtttgtcaa aattgttagt cgttatatcc gccgattttc
      961 tacctgtaag gatatttatc gtattgacga tatttacgtt ttcgttcgtt tcagctacta
     1021 cctgcaatgc cagcttcatc tcctctttgg cttctacttt ttctttcggg tttctgcaat
     1081 tatcgcacat gccacagttg ccggtttctt caccaaaata atgcaacacg aattttttgc
     1141 ggcattctcc ggtttctgcg taggcctcta cttcactcaa ctgcatcacg gctatttctt
     1201 tttcggctac cgttttttct ttattggata aaataaattt ctcgaattta tgaatttctt
     1261 tgggagaata ataggcaata caaacgcctt gcaaaccatc tctgccggct cgtccggttt
     1321 cctgataata attttcgaga gattttggaa tatcaaaatg gatgacaaaa cgcacatcgg
     1381 gcttgtctat ccccattccg aatgcaatgg tggccacaat aacttgtata tcttccatca
     1441 gaaactggtc ttgccgttca ctgcgggtat gtgcatccaa acctgcatga taggccatgg
     1501 cgttgacacc atttacggag agtacttccg ccaattcttc agtagtattt ctgctcaggc
     1561 aataaataat cccgctttta tttccttcct ttttgatata cttaatgata tcttttattg
     1621 catcttgttt gctcggtttg ggacgcactt cgtaaaatag attctcgcgc ataaaggacg
     1681 agataaaaat ttgcggatcg tgcatgccca gcgttttcac gatatcttgc tgcactttag
     1741 gtgtggctgt agctgtaagt gccactatag gaatattccc ttcaatatca ttgagcatct
     1801 gtctgatttt gcggtattcc ggacggaaat catgtcccca ttcagaaata caatgcgctt
     1861 catcaacagc tacaaaagaa attttaatcg ttttaagaaa atcaatattt tcctgtttta
     1921 ccagactttc gggagcaaca tacagcaatt tgcatttgcc gtccactata tcttttttta
     1981 cttgattggt ttgcgtttta ttttgctgag aattaataaa gtgggcaata ttatctacgt
     2041 tgctgtaacc gcgaataaga tccacctggt ttttcatcag cgcaattagt ggcgagataa
     2101 taatggcagt tccttcattc atcacagccg gcaactgata acaaagcgac ttgcctccgc
     2161 ctgtaggcat aataacaaat gtatcctttt tagatattat gctttcaatt atagcttctt
     2221 gattgccttt aaatttttca aagccaaaat attcttttaa cgcgcttaac atcgtttcat
     2281 ttttcacact cataaaaaaa tcagattaaa aatagattca aataaaatct tgttttttca
     2341 atacattaag atactcaaag ctaactaaaa caaatgcaaa aagctgttaa aaagttttat
     2401 gtaattttta cgataatatt tacttttgtg gactgatttt cagcaaataa gataaataca
     2461 agcattattc gactttttct cactataaac agttaatatg aacgcaaatg catacgtggc
     2521 catcatggca ggaggaattg gcagcagatt ttggcccgcg agcagaacag gcaaacccaa
     2581 acaatttctg gatattttag gtacgggaag aacgctgata caggcaactt acgatcgttt
     2641 tactaaaata tgccctaaag agaatatttt tattctgaca cacagtgatt atatcccgct
     2701 tatcaaagaa cagctgccgg atgtaacgga tgtacaaata ctggctgaac ctgcacgcaa
     2761 aaacacagca ccctgtattg cttacgcttc aatgaaaatc agaaaaatca atcgtgatgg
     2821 caatatgatt gtagccccat ctgaccatat tattatgaat gaagatgaat ttgtacgcat
     2881 ttgcaacacg gctttggatt ttgtatctca gcataatgca ctggctacgc tcggcatacg
     2941 ccctacacgc cctgataccg gatatggcta tatccagtat atggaaaaag aagtggcacc
     3001 tgaaattcat caggtaaaga ctttcacgga aaaaccccag aaagaagtag ctcagcaatt
     3061 tataaaaagt ggtgactttt tgtggaatgc cggaattttt atctggaatg tcaatacggt
     3121 aatttcagct ttagaaagca acttgaatga agtttatgaa gcatttgccg aaatagaatc
     3181 tgacctactc acagccaaag aacaagaagg agtagttaca gcatttacgc agtgtcctag
     3241 tatttccata gattacggta ttatggagaa agctaagaat gtatatacga taccatcttc
     3301 atttggatgg agcgatctgg gcacttgggc ttctttatat gtagagaaag aaaaagacta
     3361 cctgcaaaat gcagtaaacg gaaataacgt ggtgatttat gattctactc ataacatcat
     3421 caatgtaccc gaaaataaac tggttgtcat tcagggattg gaaaatttta tcatagtgga
     3481 taccgatgat gtgctactga tttgcaataa agacgaggaa cagcgtatca aggaatttac
     3541 gcacgacatc aagatgaata aaggtgagaa gtttttgtag ctttagcata tacaagcaaa
     3601 ctttgtatta aaatattaaa aatggtgttt tgctgtattt ggcagatgct gtattcgagt
     3661 tataacttac aatttttaac tatttcccaa atgatatttc aatatcaaat tgagctatat
     3721 ataagattgt ttttaataaa aattgctttc attaacttgt tgtatagttt aaacctcatt
     3781 gtatagttta aaccacatag aagcatagga tttataatag caaatagttg tttggtgcag
     3841 tccttttaga cagtcaagca aaaacatagt atcgcctttg gcgattccta tatttttcta
     3901 ttcttaaccc aatttttgta tggcaaacat tgataatcct aaaaaaacga tgactctatg
     3961 tggtaaaaat tataaaacaa actgtacaag aggttagtta ttaccttatt tctactaaac
     4021 aaagcaaaag gattacatta attatctcct ccttatatat agaattggtt ctgctaaata
     4081 taaaaatctc ccattttgta ttaatgcaga aaacattact ttttacggtc tccaaacttt
     4141 atttttgcac tcaaattatg ttgcaaaaga aaactggcgt tttattagtc aacctgggca
     4201 ctccggacag ccccggtaat aaagacgtgt ataaatatct cattgaattt ttgaccgatg
     4261 aacgagttat agattttggc tggctgaaaa gaaacctgtt ggtacgcggc atcatagtgc
     4321 ctgcccgtta caaacactct gcatcaactt atcgcaaaat atgggattcg gaaagaggct
     4381 ctcctttgtt atatcacaca caagatttgg gcaaactcgt acaacaacaa cttggcgatg
     4441 agtttatcgt agagattgcc atgcgctatc aaagtccctc actggaaaca gcgctggaaa
     4501 aaatgcgcca attacaagtt aacaaaattg ttgttcttcc attatttcca cagtattctt
     4561 ctgcatgcac cgggtcaata ttgcaaaaag ttcaagatat tacttccaca tggcaaacta
     4621 ttccggatat taagtttatt catcagtttt atgatgatgc tttattcata aatgcattcg
     4681 ctacacgagg aaaatcttat aatttcgacg agtacgacca tgtcgtgttt tcttatcatg
     4741 gcatccccgc caggcaaatc aagaaagcag atatcagcgg atgccattgc cttcaaaaag
     4801 aagactgttg ttcaaaaatt gaaagcatga atcagcattg ctacaaagca caatgttatc
     4861 aaactacacg tttgcttgta aaagaactta atttacctaa agaaaaatat actctttcat
     4921 tccaatcaag attgggaaaa gaagtttggc tggaacctta cacggttgat gttttacctc
     4981 agctcgcgag agaaggcaag aagaaattgc tcattttttg tccggcattt gtagccgatt
     5041 gtttggaaac actgtatgaa gtgcaagagg aatacctcga acttttccgt ggacatggcg
     5101 gcgaatcgat tacgttggta gagagtttaa attcggagcc ggaatgggta gatgctgttt
     5161 gtcatttagt aaaaaattaa agtcatgatg gttgccctgg aaaacataga acaaatactt
     5221 ctactacccg gaacagatgc ccggctaaaa gctattgcaa taaaagtgct tcatggtgag
     5281 cgcatcaaca tggaagaagg catcttgcta tatgagaaag ctaccgtagg ttttgttggt
     5341 gctttagcca attttatcag gaataaaaaa cacggtgaca acacttattt caaccgcaat
     5401 tttcatgtag agccaaccaa tatctgtgta tatacttgca aattttgttc gtattctcgt
     5461 ttgataaaac agcgcgaaca aggctgggaa ctcagtgtag aacagatgtt ggatattgta
     5521 aaaaaatacg acaatcaacc ggtaacggaa gtgcatatca caggcggtgt tatcccaaaa
     5581 caggattttg aattttatca aaacttattc agtgctataa aaaaacatcg tcccggtttg
     5641 catatcaaag cacttacccc tgtagaatat cactatattt tcaaaaaagc aaaagttacg
     5701 tacgaagaag gattaagagc aatgaaagcc gcaggattgg attcgatgcc gggtgggggt
     5761 gccgaaatat ttgacgataa aatccgcgat gaaatagcag gtggaaagtg cagcgcgcaa
     5821 gagtggctgg atatgcacga aacagcgcat aagcttggca taccgagcaa tgccacgatg
     5881 ttgtatgggc atatagaaac tttcgcacat cgtgtaaatc acatggagcg actgcgccaa
     5941 ctgcaagacc gcacacatgg attcaatgca ttcattccgc tgaaattcag aaaccaacac
     6001 aatgaaatgc agcatttacc ggaagtaact gtgattgaag acttgcgcaa ttatgctatc
     6061 gccagaatat atctggataa tttcgatcat ataaaatcct attgggccat gataggcaga
     6121 aataccacgc aacttagctt gaatttcggc gtggatgata ttgacggcac gatagacgat
     6181 acgacaaaaa tctattcgat ggcgggcagc gaagaacaag ctccggctat gagtactcgc
     6241 gagctggtga acctcatcaa agctgtaggc agaaaaccta tagaacggga tacggtttat
     6301 catgtcatta ctgattattc tgatattgta tttgaagaag atgaaaaaga aaaagcgaga
     6361 aactattaat atattttaca tgaaatctat atatctactt cttttattgg tatttccttg
     6421 tgttttattc gcccaggatc taaccgaagt caactcatca gaaaagcctg ttaaagaaaa
     6481 gaaatttatt ttccgggcgg gtaccatgtc gggcttaaat tt
//
LOCUS       DAOQII010000135         6500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6647_length_6500_cov_4.820791, whole genome shotgun sequence.
ACCESSION   DAOQII010000135 DAOQII010000000
VERSION     DAOQII010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6647_length_6500_cov_4.820791"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLF48_07460"
     CDS             <1..913
                     /locus_tag="PLF48_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49197.1"
                     /translation="FTYKNIKWQMAARYEYTRFLPAFYDATKILQKPVFNFSNYGAGI
                     YLSYTQPKNYFVLSSSLTANSRFPNPGELYSNGLHHGAAVMEYGNPNLQNEHNIKFAI
                     GLQSNYKQYLHVEVNPYINYSKNYIYYYPLGYPVLTIRGAFPAFKYTNTNALLYGADF
                     YARSNFYKYFTLWMKSSLVRGRNMNTRDNLIYMPSDQLSFGLDVKAEFKHSNYFYGTI
                     NTETVIKQTRIPSDITDYKVAPPTYTLLNIEAGYSMKIKTKNALQFSVSAENAANKSY
                     RNYLNRFRYYTDEPGANIIFRIKYAFE"
     gene            916..1488
                     /locus_tag="PLF48_07465"
     CDS             916..1488
                     /locus_tag="PLF48_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49198.1"
                     /translation="MKSTKVIKVVCLTISMLVVFGCKKKDPVLINEAELITTVKVVFT
                     DTLTNQNYTFQWEDLDGDGASQPIITSDTLPKNTYFTTQIELWNKQNNPEENITTEVE
                     EEGIAHQFFYSVSPGTLLQYFNYEDSDEDGKPIGLIFSCKSNGTGGEGMFKLILRHLP
                     DKFALGVSTGDITNANGETDVEIEYPFVIK"
     gene            complement(1503..2990)
                     /locus_tag="PLF48_07470"
     CDS             complement(1503..2990)
                     /locus_tag="PLF48_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49199.1"
                     /translation="MNKIKLFFSDRVLKYLVFLLFAIGVLYRVILWWMNRGMYIDEAN
                     LARNIYERNFLGLTRPLSYEQFAPPFFLFITKINTILFGSDEKVFRLFALISGVAAIF
                     LFVKIARQFISLKAIWYPLALFATGAIYLRYSTEFKQYSSDAFISLLVVYTATRLSIN
                     TAKPILFLMVWILLGSLAIWLSMPSVFILAGVGAYYFAEIIRTKTYKQSYLLIFIVLI
                     WLLQFGYYYFAILQPQSQSNYLQQFHHWYFLDAFPESFNAFKSHNGLLLKGLFEAMSG
                     KLLLSLILNIILYITGVYYTIKANRNLLFLLFFPIILTLAASALKAYSLMDRLMLFYF
                     PLGLLLLGKGLDFFMQKVNAVVIKIPVILLCVFTVFKFNKISYLTTKPLNLEAVDDCL
                     DFLLTQNITAQNLYIHNLASGGYVYYTEIHPNKTKWKSLANAHFLNWDADYTQLLANE
                     KSAVLTTSMGQDVKEKVWDKIVISNTILATYDKTDAKAMVFEAKK"
     gene            complement(3006..4229)
                     /locus_tag="PLF48_07475"
     CDS             complement(3006..4229)
                     /locus_tag="PLF48_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNF49200.1"
                     /translation="MKKLVLAVSIFIAYNLQAQVKLDKGIMMEKKNAFYDSIIRMNDA
                     YSEPKSASKKMELKMDFSGLDYPKSINDFKTIKAINPVSQGNTGTCWSFSSISFYEAE
                     IARLTGSVIDLSEMYNVYWQYVEKAKEFVRTRGTSVFDEGSESNATQLMMKKYGTVPA
                     YAYTGMKDGQPFQDHSKMADEMKAYLQSIKNNNAWNTDEVVVTIKSIMNFYMGTPPTT
                     VEWKGVNMSPQDFMKNICKLNPDDYVELMSFANVPYWTKQEYAVPDNWWHSKDYINIP
                     LDVYMSTLKKAAANGISAVIGGDVSNEPGMYNKFGIAVVPSFDIPSAYIDENARLFRF
                     LNGTTTDDHGMHMVGYCEKPNGIWFLIKDSGSNGHNNINADGYWFMHEDYVKLKMTTY
                     TVPKSLVKDILEKVK"
     gene            4417..>6500
                     /locus_tag="PLF48_07480"
     CDS             4417..>6500
                     /locus_tag="PLF48_07480"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF49201.1"
                     /translation="MNKKIYTLLWCTITTLLAIAQPANDNCTNAILISNVSNYCSAAA
                     AGTTVAATDDVATGGGFAAAACWGGTVNDVWYKFVATATDVTIVINGNQGSPLGGTLA
                     RPQVALYSGTCGGTINELVCGSAPAGQNIIQVYKGGLTIGETYLIRVDGFNTNKGTFQ
                     YCINNYNPVPTPSSDCPTAVVLCDKSTFTVPAVSGAGANNTEMNDAACFGGGTIESNS
                     TWYAFTFATSGTFNFTLFPSNTNDDLDFVVYKLPNGLGNCSGKQVVRCMASAPLCTPG
                     GANANTSTTGLSPTAGYNEEYSGCNADLAYFWCGIGSNRCQSQFVKELTVVAGETYAI
                     AINNFTSTGNGFTITFGGTAQIQGPVANINDNDADDIICPGEAITFTDASTPPPAGTL
                     TQWTWNFGSGATPATFNGQNPPAVTYSTTGTKTITLTVKSNRGCLVTSTKTITVANTP
                     PTVAIVATAISICPGTNVTFTATPSNAGSNPTYQWYLNGTPVGTNSSTYSNNALPNNA
                     QVYVKVTSNGICNSGLTANSNTITITHTTAAAVSVSITGNAPICQGTSLTLTANPTNG
                     GTVPSYQWYINNIAVGTNSSTYTSATFNNNDVIKVVLTSDLSCTSGNPATSPTITITV
                     KPNPTVTVNSPSICNGQSATLTASGATTYSWNTGATGASISVSPTTTTTYTVTGTTNG
                     CDGTATSTVTVKP"
BASE COUNT         2136 a         1198 c         1171 g         1995 t
ORIGIN      
        1 atttacgtat aaaaatatca aatggcaaat ggcagctcgc tatgaatata ccaggttttt
       61 gcctgctttt tatgacgcca caaaaatttt gcaaaagccc gtttttaatt ttagtaacta
      121 tggtgcggga atttatcttt cttatacaca gcccaaaaat tatttcgttt tatcctcttc
      181 gctaacagca aattcacgct ttccgaatcc gggagaatta tacagcaacg ggttacacca
      241 tggagctgcg gtaatggagt atggaaaccc taacctacaa aatgagcata atattaaatt
      301 tgcaataggt ctgcaaagta attacaaaca atatttgcat gttgaagtaa atccgtatat
      361 caattacagc aaaaactaca tttattatta tccgctcggc tatcctgtat taactattcg
      421 cggtgcgttt ccggcattca aatataccaa taccaatgcg cttttatatg gagcagattt
      481 ttatgccagg agcaattttt ataaatactt taccttgtgg atgaaatcga gtttggtgcg
      541 aggcagaaat atgaacacta gagataactt gatttatatg ccgtccgacc aacttagttt
      601 cggattagat gtaaaagcgg agttcaaaca ttccaattat ttttatggaa cgattaatac
      661 cgaaacggtc atcaagcaaa cacgaatacc aagcgatatt acagactata aagttgcacc
      721 gcccacttac acattattga atatagaagc aggatattcg atgaaaataa aaacaaaaaa
      781 cgccttgcaa ttttccgtat cggcagaaaa tgctgccaat aaatcgtaca gaaactattt
      841 gaatcgcttt cgctactaca cagacgaacc cggagcaaat atcattttta gaataaaata
      901 tgcatttgaa taagtatgaa atcaactaaa gtaataaaag tagtttgctt aacgataagt
      961 atgctcgtgg tttttggctg taaaaagaaa gatcctgttt tgataaacga agcagaatta
     1021 atcactacag ttaaagttgt ttttactgat acgctgacta atcaaaacta taccttccaa
     1081 tgggaagatt tggatggaga tggagccagc caaccaatca tcacttctga tactttgcca
     1141 aaaaatacat attttacaac gcaaatagaa ttatggaata aacaaaataa tcctgaagaa
     1201 aatattacca cagaagtgga ggaagaaggt atagcccatc agttttttta ttcggtttct
     1261 ccgggtactt tgttgcaata tttcaattat gaagattccg acgaagatgg aaaaccaata
     1321 ggacttattt ttagctgcaa aagtaatgga acaggaggag agggtatgtt taaattgata
     1381 ctaaggcatc tccctgataa gtttgcatta ggagttagta caggtgatat taccaatgcc
     1441 aatggagaaa cagatgtgga aattgaatat ccatttgtaa taaagtaaac actaattaac
     1501 ggttactttt tagcttcaaa taccattgct ttagcatctg ttttatcgta agtagctaga
     1561 attgtattag atattactat tttatcccag actttctctt ttacatcttg tcccatagaa
     1621 gttgtcagta ctgcagattt ttcatttgca agaagttgag tgtaatctgc atcccagttg
     1681 agaaagtgag catttgccag tgatttccat tttgttttat tcgggtgaat ttctgtatag
     1741 tacacgtagc ctccacttgc caaattatga atatataaat tttgtgcagt aatgttctgg
     1801 gtaagcagga agtctaaaca atcatcaaca gcttctagat taagtggttt ggttgtgaga
     1861 taactgattt tgttaaattt aaatactgta aatacacaca acaatatgac aggaatttta
     1921 attacgactg cattcacttt ttgcataaaa aagtctaatc cttttccgag cagtaataag
     1981 cctaatggaa aataaaaaag cattaacctg tccattaatg aataagcctt caaagcggat
     2041 gcagcaagag tcaatattat aggaaagaac aataagaata ataaattcct gttggcttta
     2101 atagtataat atacccctgt tatatataat atgatattga gaattaaact tagcaacaac
     2161 ttacctgaca tggcttcaaa aagtcctttt agcaataagc cattatgaga tttaaaagca
     2221 ttgaaacttt caggaaatgc atctaaaaaa taccagtgat gaaattgctg caagtaatta
     2281 gactgcgatt gtggttgtaa tatggcaaaa taataatagc caaattgcaa gagccaaatt
     2341 agaactataa atatcagaag atatgattgt ttgtaagttt ttgtcctgat gatttctgca
     2401 aaataataag ctcctactcc tgcaagtata aacacggacg gcatggacag ccatatagct
     2461 aaagagccca gcagaatcca taccatcaaa aacaatatcg gtttcgctgt atttattgac
     2521 aatcgagtgg ctgtatatac tacgagtaaa gagatgaaag catctgaaga atattgttta
     2581 aattctgttg aatagcgcaa atatattgct cctgttgcaa acaatgccaa tggataccaa
     2641 attgctttta gcgaaataaa ctgtcttgca attttcacga aaaggaatat ggctgcaact
     2701 cctgaaatca atgcaaacag tcgaaatact ttttcatcag aaccgaataa gatggtgtta
     2761 atttttgtga tgaacaagaa gaagggagga gcaaattgct cgtagctaag tggtcgggtc
     2821 aaacctaaaa aattgcgctc gtaaatattt cgagccaagt tagcttcatc aatatacata
     2881 cctctgttca tccaccagag tatgacacga tagagtacac caattgcaaa aagcaagaat
     2941 actaaatact ttagaacacg gtcagaaaaa aaaagtttta tcttattcat tctgacaaaa
     3001 tgagcttatt ttaccttttc taaaatgtct tttaccaaag attttggaac ggtataagtg
     3061 gtcattttaa gtttcacata gtcttcatgc ataaaccaat aaccatcagc atttatgtta
     3121 ttatgcccat tgctaccgct gtcttttatc aaaaaccaga tgccgtttgg tttttcgcaa
     3181 tatcccacca tgtgcatacc gtggtcgtcg gtagttgtcc cattcaggaa acggaataaa
     3241 cgggcatttt catcaatata agctgaagga atatcaaatg atggcactac agcaatacca
     3301 aatttattat acatgcccgg ctcattactt acatcaccgc caatgacagc agaaattcca
     3361 tttgctgctg cctttttcaa agttgacata taaacatcaa gcggtatatt gatataatct
     3421 ttagaatgcc accagttatc cggtactgca tattcttgtt tggtccaata aggcacattg
     3481 gcaaatgaca ttaattctac ataatcatcc gggttgagtt tacagatgtt tttcataaaa
     3541 tcttgaggcg acatatttac acctttccat tccactgttg tcggaggtgt acccatgtaa
     3601 aagttcataa tagactttat cgttactaca acttcatccg tattccacgc attattattt
     3661 tttatacttt ggagataggc tttcatttca tcagccattt ttgaatggtc ttgaaaaggc
     3721 tgaccgtctt tcatgcctgt ataagcataa gcaggaacag tgccgtattt tttcatcatc
     3781 agttgggtgg catttgattc ggagccttca tcaaaaacgc tggttccacg ggtgcggaca
     3841 aattcttttg ccttttctac gtattgccag tacacgttat acatttcaga aaggtcaatc
     3901 acactaccgg tcaaacgggc tatttctgct tcgtaaaatg atatggagct aaagctccag
     3961 caagtgcctg tgttgccttg agatacagga ttgatggctt ttatcgtttt aaaatcgttt
     4021 atagatttag gataatccaa accggaaaag tccattttca attccatttt tttagacgcg
     4081 gattttggtt cgctgtaagc atcattcata cgtataatgg aatcataaaa tgcattcttt
     4141 ttttccatca tgatgccttt gtcaagtttt acttgagctt gtaaattgta ggctataaaa
     4201 attgatacag ctaaaactaa ttttttcatt cttattaaat tgtacacaaa agtaagaaat
     4261 ttcgctctaa ctatcttgtg tttgacagtt agagattagc agtattaatt aattaagcta
     4321 ttccatgttg ataaaattca tattgttagt tatgaatagg ggcaactttt aataattcag
     4381 taattttttt ttatttttaa ggaggaaaac tgacttatga ataagaaaat atacacactg
     4441 ctatggtgta cgataacgac gttattagca attgctcaac ctgcaaacga taattgtaca
     4501 aacgccattt tgatatccaa tgtatccaat tattgctcag ctgccgcagc cggaaccact
     4561 gtggctgcta ctgacgatgt ggcaacaggt ggaggttttg cagccgcagc atgctggggt
     4621 ggaaccgtaa atgatgtgtg gtataagttt gttgccacag cgacagatgt aacaatagta
     4681 attaatggaa atcaaggctc tcctctagga ggaacattgg cacgaccaca agttgcctta
     4741 tactccggta catgtggggg gacaattaat gaattagtat gcggttctgc acctgcagga
     4801 caaaatatta ttcaggttta taaaggtgga ttaacaatag gtgaaactta tttaattcgt
     4861 gtagatggct ttaatacaaa caaaggtaca ttccaatatt gtattaataa ttacaaccct
     4921 gttccaactc ctagtagtga ttgcccaaca gccgtcgtgc tttgcgataa aagtacattt
     4981 actgttccgg ctgtaagcgg agctggtgca aataacacag aaatgaatga tgctgcttgc
     5041 tttggaggcg gaactattga gtcaaactct acttggtatg cttttacttt tgctacatcc
     5101 ggtacattta actttacttt gtttccttcc aatacaaatg atgatttaga ttttgtagtt
     5161 tataaattgc ctaatgggct aggtaactgt agcggaaaac aagtagttcg ttgtatggcg
     5221 tctgccccac tatgcactcc gggtggagct aatgcaaata cctctactac tggactatct
     5281 ccaacagctg gttataatga agaatattcc ggttgcaatg cagacttagc ttatttttgg
     5341 tgtggtatag gaagcaacag atgtcaaagt cagtttgtaa aagagcttac tgttgtagct
     5401 ggtgaaactt acgctattgc catcaataat tttacttcta caggaaatgg atttactatc
     5461 acatttggcg gtacagcgca aatacaagga cctgtagcta atataaatga caatgatgcc
     5521 gatgatatta tttgtcctgg tgaggcaatt actttcacag atgcttctac accgccaccg
     5581 gcaggtacac ttacacaatg gacttggaat tttggctctg gagctacacc tgcaactttc
     5641 aacggacaaa atcctcctgc agttacatac tctacaaccg gaacaaaaac tattacttta
     5701 acagtaaaaa gcaacagagg ttgtttggtt acttcgacta aaacaattac tgtggcaaat
     5761 actcctccaa cagtagctat tgtagctaca gcaatttcaa tttgtccggg tacaaatgtt
     5821 accttcactg ccactccatc caatgcagga tcaaatccaa cctatcaatg gtatttgaat
     5881 ggcacacctg taggtactaa ttcttcgact tattcaaaca atgcattgcc caataatgct
     5941 caagtatatg taaaagtaac aagcaatggt atttgtaaca gcggattaac tgccaatagc
     6001 aacactatta caataactca tactactgcc gctgctgtat ccgtaagcat tacaggtaat
     6061 gcaccaatat gtcaaggcac atcattaact ttaactgcta acccaacaaa tggcggaact
     6121 gtaccttctt atcaatggta tataaataat attgcagttg gcacaaattc ttcaacatat
     6181 acttcagcaa catttaacaa taatgatgtc ataaaagtag ttttaacgag tgatttaagt
     6241 tgtacaagcg ggaatcctgc aactagtcct actattacga taacggtaaa acccaatcca
     6301 acggtaacag taaacagccc ttccatatgt aatggtcaat ctgctacact tactgcaagt
     6361 ggagcaacta cctatagttg gaataccggt gcaacaggtg caagtatttc tgttagtcca
     6421 accacaacga caacttatac cgtaacaggt acaactaatg gttgtgatgg aactgccaca
     6481 tccactgtaa cggtaaaacc
//
LOCUS       DAOQII010000136         6473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6682_length_6473_cov_4.747273, whole genome shotgun sequence.
ACCESSION   DAOQII010000136 DAOQII010000000
VERSION     DAOQII010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6682_length_6473_cov_4.747273"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLF48_07485"
     CDS             complement(<1..607)
                     /locus_tag="PLF48_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49202.1"
                     /translation="MKKCLLFGIACMLFACKKETTKQTLRTASSADIKTESWMHDLQQ
                     LYASSNFTLKDIMIPAAHDAGMYQLTSCTFGANACNTQTQYQSFQKMLMQGIRMFDVR
                     PKKVGNKFYTFHATDCDGLGCNGAPLASIYSDINTFLNSHAELVVLELSHFCHTAFTD
                     TALINLAERTFGAKLLKDTNPVPLNFLTTPLAELINSNNTGK"
     gene            complement(896..1243)
                     /gene="gldC"
                     /locus_tag="PLF48_07490"
     CDS             complement(896..1243)
                     /gene="gldC"
                     /locus_tag="PLF48_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HNF49203.1"
                     /translation="MVRETEIKFKIQLDNQNLPEKIKWTATDGPQGDGDECKSIFVGM
                     WDGNEKNTMRFDLWTKDMQIDEMHTHFFQLLLSMGDTYHRATGNPFVQEDIKQFCMDL
                     ADKTRAWEDKNKD"
     gene            complement(1551..2801)
                     /locus_tag="PLF48_07495"
     CDS             complement(1551..2801)
                     /locus_tag="PLF48_07495"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNF49204.1"
                     /translation="MRVFKFGGASVKDANGIKNVAGIIQKYGDKNLVIVVSATGKTTN
                     ALEEVIKAYFSDADPKEKFEIVKQNHNQIAQELFGHQHPVFDKLQNIYAEVEWQLEED
                     RKDAYNYVYDQIVSQGEQISSVILSAYLNEVGVVNTWLDVRDIMKTDDTYREAIVDWQ
                     KTQQLMNEKVKPVLNDTIMVTQGFIGCTSENCTTTLGREGSDYTAAIFANMLDAENQT
                     IWKDVPGVMNGDPRVFEDAIFMDEISFNEAVEMTFYGATVIHPKTIKPLQNKNIPLLV
                     KSFINPEGKGTTVGGSDSNLPPVRIVKNQQALISLHTKDFSFVEDEIVAEIFNAFSKA
                     NLKVNMMQQGAIKFQAVVDNIPEKIQKVKAALDAHFNFEISEGLTILTIRHYPDFECI
                     KKHIGDKTALLEQRTKDTYQGLLK"
     gene            complement(2846..3310)
                     /locus_tag="PLF48_07500"
     CDS             complement(2846..3310)
                     /locus_tag="PLF48_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49205.1"
                     /translation="MITVRKAEPEDFEVIYSLIHELAVFEQAPDEPSNSLKRFIEEGT
                     CKNPRFHIIVAEANKEIAGMALYYFGYSSWKGSMLYLDDLIVRENYRRQGIGKLLMDE
                     LIKIAKEEKINQLRWHVLDWNESAINFYKNYPVSFDKTWITVKIEKDDLSSI"
     gene            3632..6232
                     /gene="clpB"
                     /locus_tag="PLF48_07505"
     CDS             3632..6232
                     /gene="clpB"
                     /locus_tag="PLF48_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNF49206.1"
                     /translation="MNPNNFTIKTQEVLADAQQIAFDLKHAYMDTFHLMKAILNNDED
                     MTPFLFKKTGVDIKKIEQELSALIQKQPTVSEDNVKYPSQNLSQVLLKANSYLKELND
                     EYVSIEHLLLALLSVNDETSKILKQNGLTEANLKQAIKDLRKGSTVNNPSADMTYNSL
                     DKYAKNLNQLAESNKLDPVIGRDEEIRRVLHILSRRTKNNPILVGEPGVGKTAIVEGI
                     AFRIIQGDVPENLKTKIIYSLDMGALIAGAKYKGEFEERLKSVIKEVISSEGQIILFI
                     DEIHTLVGAGGGGDGAMDAANILKPALARGELRAVGATTLNEYQKYFEKDKALERRFQ
                     KVMVQEPSVEDTVSILRGLKERYENHHKVTIKDEAIISAVELSNRYITNRFLPDKAID
                     LIDEAAAKLRLEMDSVPEELDELERKIRQLEIEREAIKKENDEAKVADLSKQIAELSE
                     RRNEFKAKWESEKTVVDRIQNEKIKIENLKLEAEQAERNGDYGKVAEIRYGKIKDSEE
                     KLAEFEKELQENKADKRLMKEEVTSEDIAEIISKWTGIPLTKMLQTEKDKLLNLEDYL
                     HLRVVGQDEAIVAVADAIRRSRAGLHDPKKPLGSFLFIGTTGVGKTELARALAELLFD
                     DEKAMVRIDMSEYMEKHSVSRLIGSPPGYVGYDEGGQLTEAVRNHPYSVVLLDEIEKA
                     NPDVFNILLQVLEDGRLTDNKGRVVDFKNTIIIMTSNIGSHLIQQNFENVKTESEVFT
                     ATETSKIQILDAIKRVLRPEFYNRIDEVIMFKPLLQNDIKDIVRIQLHNLQKQLKDME
                     IDLSYTDELTNHFAEEGYDVQFGARPLKRLINRELINQLSKLIIADKIKKDKPVIADI
                     FDGEIVFRNE"
     gene            complement(6240..>6473)
                     /locus_tag="PLF48_07510"
     CDS             complement(6240..>6473)
                     /locus_tag="PLF48_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF49207.1"
                     /translation="KIHATFSGHTHGFQFGVENPKMHIKWSPSQWVYPHWAGLYNNER
                     GQYLYVNRGAGFLGYPGRVGIPPEITIIQFKKK"
BASE COUNT         2108 a         1195 c         1190 g         1980 t
ORIGIN      
        1 cttttccggt gttgttgctg tttatcaatt cagctaatgg tgtcgttaga aagttgagcg
       61 gtacagggtt cgtgtctttt aacaatttag caccaaacgt gcgctcagcc agattgatta
      121 aagcggtatc tgtaaatgcg gtatggcaaa aatgggataa ttccagcact acaagctcgg
      181 catgactgtt tagaaatgta tttatatcgc tgtagataga agcaagtggt gcgccattgc
      241 agcccagtcc gtcacagtcg gtagcatgaa aagtgtaaaa cttattcccg acttttttgg
      301 gacgcacatc aaacatacgt atgccctgca tcagcatttt ttgaaacgac tggtattgtg
      361 tttgcgtatt gcaagcatta gcaccaaacg tacatgaggt aagctgatac atgcctgcat
      421 catgtgcagc cggtatcata atatctttta atgtaaaatt agatgatgca taaagctgtt
      481 gtaaatcatg catccagctt tctgttttta tatcagcaga tgaggccgtt cgcaaggttt
      541 gttttgttgt ttcttttttg caggcaaaca gcatacatgc gattccaaaa agcaggcatt
      601 tcttcatctt ctaatttaaa agaaaaaatg aaaataaaaa agaaaggaaa ggagataaaa
      661 cctgactagc ctttagtttt tgtccggttt gatttatagt gtattaccat atagaggcat
      721 agattttttt agaattaaca atgcttacca tacaataatt gagaaagaat agaaaaatat
      781 agaaatcgcc ataggcgata cgctattttt gcttgactgt ctaatcagat tttcgctaag
      841 cagcaaatgt ttgattaaat gctatgtttc tatgtgattt aaaccataca actagttagt
      901 ccttgttttt gtcttcccaa gcacgggttt tatctgccaa atccatgcag aactgcttga
      961 tatcttcttg tacaaaagga ttgccggtag cacgatgata agtgtcgccc atacttagca
     1021 acagctgaaa aaaatgcgta tgcatttcat ctatttgcat atcctttgtc cacaaatcaa
     1081 agcgcatggt atttttttcg tttccatccc acatgcctac aaaaatactt ttgcattcat
     1141 caccgtcacc ttgcggaccg tcagttgccg tccattttat tttttctggc agattttggt
     1201 tgtccagttg tattttaaat tttatttcag tttctcttac catgttaact tgtttaggtt
     1261 gtttatcgtt cgcaaaaata agatttacta atggatttag tgcagattaa tctgcaatac
     1321 tattatttat tactcatttt caatggatta aatgagcagt aagccgagtg tggctaaagt
     1381 atttttcatg gaatatattt gaaagtatat aagaactacc tcatctttaa tccccccgta
     1441 aaactgtcaa cttatttcaa gtctgaatag tatgaaaata aatttatgta gcatacttgt
     1501 atgtcaatat tcatcagtgg actgcgcact atggactatc gactatcagc ttacttcaac
     1561 aacccctgat aagtatcttt agtacgctgt tcaagcaaag ccgttttatc tccaatatgt
     1621 tttttgatac attcgaaatc gggatagtga cggatagtca aaatggtgag cccttcagaa
     1681 atttcaaaat taaaatgtgc atcaagagcg gccttaactt tttgtatttt ttcaggaata
     1741 ttatcaacga cagcctgaaa tttgatagct ccttgttgca tcatatttac cttgagatta
     1801 gcttttgaga atgcgttgaa gatttctgct actatctcgt cttctacaaa tgaaaaatct
     1861 ttggtatgca atgaaataag tgcctgttgg ttcttcacga tacgcaccgg aggtaaattg
     1921 ctgtccgaac cacctactgt tgtcccctta ccttcaggat tgataaatga tttgaccaat
     1981 aagggaatat ttttattctg gagtggtttt atcgttttgg gatggataac tgttgctccg
     2041 tagaaggtca tttccacagc ttcgttaaat gaaatctcat ccataaaaat ggcatcttca
     2101 aatacacgcg ggtcgccatt catcactccg ggtacatctt tccaaatggt ttggttttca
     2161 gcatcaagca tattggcaaa aattgctgcc gtataatcag agccttcccg gcccaaagta
     2221 gttgtacagt tttcagatgt gcagccaata aaaccctgtg taaccataat cgtatcattc
     2281 aaaacaggtt tcactttttc gttcatcagc tgttgtgttt tttgccagtc tactatggcc
     2341 tcccggtaag tatcatctgt tttcatgata tctcttacat ccagccaggt atttacaacg
     2401 cctacttcat tgagatatgc acttagaatt acagatgaaa tctgctcacc ttgtgataca
     2461 atctggtcat aaacatagtt gtatgcatct ttgcggtctt cttcgagttg ccattctact
     2521 tcggcataaa tattttgtaa tttatcaaaa acagggtgct gatgaccaaa taattcctgt
     2581 gcaatctgat tgtgattttg ctttacaatt tcaaattttt cttttggatc tgcatccgag
     2641 aaataggctt taattacttc ttcgagtgca ttagtcgttt ttccggtagc tgaaacgaca
     2701 attactaaat ttttatcgcc atatttttgt atgattccgg caacattttt tatcccgttt
     2761 gcatctttta ccgatgctcc tccaaattta aatacacgca tctttctatg attaaaattt
     2821 tataattaat agttatcgta tcagattaaa tactactcaa atcatctttt tcaattttca
     2881 ctgttatcca tgttttgtca aaactaaccg gataattttt ataaaaattg atagccgatt
     2941 cgttccagtc taatacatgc cagcgcaact gatttatctt ttcttccttg gcaatcttta
     3001 ttaactcatc cattaataat tttccaatgc cttgtcttct gtaattttca cgaacaatca
     3061 aatcatccag atataacata gagcctttcc aggaggaata gccgaaataa tagagtgcca
     3121 taccggcaat ttctttattc gcttctgcta ctattatatg aaacctcgga tttttgcaag
     3181 ttccttcctc aataaatcgt tttagagaat ttgaaggttc atcaggagcc tgctcaaaaa
     3241 cagccagctc gtgaatcaaa gaataaatca cctcaaaatc ttccggttcg gcttttctaa
     3301 cagttatcat cacttttgct atatctattg cgaagataaa aaatcaaaaa gcataattca
     3361 ttaagtaaac atcaaatagt cagtaaacta aatagcgaag caaacataaa aaatgtcagg
     3421 ttttatttaa aaacaatgac atatttacag cataactata aaaaaactga aaaattttct
     3481 gaaataattc aatttcatcg ttagcataat tcttgataaa atagtctata aaaatccaac
     3541 gctgcttctc ttaaaaataa actaccgaat tatacctaaa acaaatgggt atttcgtaat
     3601 ttagtattat tcactattaa actattaaaa aatgaatcca aataatttta ccattaaaac
     3661 gcaagaggta ctggcagatg cccagcaaat tgcattcgat ttaaaacatg cctatatgga
     3721 tacttttcat ttgatgaaag ccatcctgaa taatgatgaa gacatgactc cttttttatt
     3781 caaaaaaacc ggtgtggata tcaaaaaaat tgaacaagaa ttaagtgctt taattcaaaa
     3841 gcaacctact gtttcggaag ataatgtgaa atatccttct caaaatctaa gtcaggtttt
     3901 attgaaagct aattcttatt tgaaagaact aaacgacgaa tacgtttcta tcgaacattt
     3961 attattagct ttattaagtg tgaatgatga aacctcaaaa attttaaaac aaaacggtct
     4021 taccgaagct aatctgaaac aagcaataaa agatttgaga aaaggctcaa ccgtaaataa
     4081 tcccagcgca gatatgactt acaattcatt agacaaatat gcaaaaaatc taaaccagct
     4141 cgcagaatcc aacaagcttg atcctgttat tggcagagat gaagaaatac gccgtgtatt
     4201 gcacatctta tcccgacgta caaagaacaa cccgatttta gtgggcgaac ccggcgtagg
     4261 taaaacagct atcgtagaag gtattgcttt tcgtatcatc caaggcgatg taccagaaaa
     4321 cctgaaaact aaaatcatct attcattgga tatgggtgcc cttattgcag gtgccaaata
     4381 caaaggggaa tttgaagaac gtttgaaatc tgttatcaaa gaagttattt catctgaagg
     4441 acaaatcatc ttgtttattg acgagattca tactttagta ggtgcaggtg gcggtggcga
     4501 cggtgctatg gatgctgcca atattttgaa acctgctctt gcccgcggtg agctgcgtgc
     4561 cgtaggcgct accacactca acgagtatca aaagtatttt gaaaaagaca aagcattaga
     4621 acggcgtttt cagaaagtaa tggtacaaga acccagtgtg gaagatacag tttccatttt
     4681 gcgcggactg aaagagcgct atgaaaacca tcataaagta acgataaaag acgaggccat
     4741 tatcagtgca gttgagcttt ctaaccgtta tattaccaac cgctttttac cggacaaagc
     4801 aatagactta attgatgaag cagctgctaa gcttcgttta gaaatggact ctgtgccgga
     4861 agagctggat gaactggaac gaaaaattcg tcagttggaa atagaacgtg aagccattaa
     4921 aaaagaaaac gatgaagcaa aagttgcaga cttgtctaaa caaattgctg aactgagtga
     4981 acgcagaaac gagtttaaag caaaatggga aagtgagaaa acggttgtag acagaattca
     5041 aaatgagaaa ataaaaattg aaaatctaaa actcgaagct gagcaagcag aacgcaatgg
     5101 cgactatggc aaagtagccg aaatacgata tggcaaaata aaagacagcg aagaaaaact
     5161 ggcggaattt gaaaaagaat tgcaagaaaa caaagcggat aaacgcttga tgaaagaaga
     5221 agtaacctca gaggatattg ctgaaatcat ctcgaaatgg accggtatac ctttgacaaa
     5281 aatgttgcaa accgagaaag acaaattgct gaatttagaa gattatctgc acctgagagt
     5341 ggtaggtcag gatgaagcta tcgtggctgt tgccgatgcc attcgcagaa gccgtgccgg
     5401 gttacatgat cctaaaaaac cattaggctc gtttttattt atcggtacta cgggtgtcgg
     5461 taaaacagaa ttagcccgag ctttagccga gttattattt gacgatgaaa aagccatggt
     5521 gcgtatcgac atgagtgaat acatggaaaa acattccgta tcccgactga taggttcgcc
     5581 tccgggatat gtaggctatg atgaaggtgg tcagcttaca gaagcggtac gcaatcatcc
     5641 atattcggta gtgttgctgg atgaaattga aaaagcaaat cccgatgtat tcaatatatt
     5701 gttacaggtg ttagaagacg gcagattaac tgataataaa ggtagagtag tcgattttaa
     5761 aaacacgatt attatcatga cctccaatat cggctcgcat ttgatacagc aaaattttga
     5821 aaatgtaaaa actgaaagtg aggttttcac cgctacggaa acttctaaaa tacaaattct
     5881 ggatgccata aaacgggtat tacgtccgga gttttacaac cgtattgatg aagtcatcat
     5941 gtttaaacct ttactgcaaa atgacatcaa agacatcgtt cgcattcagt tgcacaactt
     6001 gcaaaaacag ctgaaagaca tggaaattga tttgagttat accgatgaat taaccaatca
     6061 ttttgctgaa gaaggctacg atgtacaatt cggtgcaaga ccactgaaga gattaatcaa
     6121 cagagaactt atcaatcaac tgtcaaaatt gattattgcc gataaaataa aaaaagacaa
     6181 gcctgtgatt gccgatattt tcgacggaga aatcgtattc agaaacgaat aaacagcaat
     6241 tattttttct taaattgtat aatcgtgatt tccggtggaa tgcccactcg accgggataa
     6301 cctaaaaatc cggcaccacg attgacatat aaatactgtc ccctctcatt gttgtataag
     6361 cctgcccaat gcggatatac ccactgtgag gggctccatt ttatgtgcat tttcggattt
     6421 tcaactccga actgaaagcc gtgtgtatgc ccggaaaatg tagcatgtat ctt
//
LOCUS       DAOQII010000137         6447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6741_length_6447_cov_6.280820, whole genome shotgun sequence.
ACCESSION   DAOQII010000137 DAOQII010000000
VERSION     DAOQII010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6741_length_6447_cov_6.280820"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            515..1888
                     /gene="leuC"
                     /locus_tag="PLF48_07515"
     CDS             515..1888
                     /gene="leuC"
                     /locus_tag="PLF48_07515"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNF49208.1"
                     /translation="MGKTLFDKIWDSHIVEAIENGPQVIYIDKHFIHEVTTPQAFEGL
                     RKRSIPVFRTQNTVATADHNVPTKDQNLPIREALSKHQVDTLIQNCTDFGIELYGLGH
                     PFQGIVHVIGPELGITQPGMTIVCGDSHTSTHGAFGAVAFGIGTSEVEQVLATQCILQ
                     TRPKTMRINIDGELGKGVTAKDITLYIISKISTGGGTGYFVEYAGSAIRSLSMEARMT
                     VCNMSIEMGARGGIIAPDETTFAYLKGRTFAPENYEQALEKWQTLFTDEDAAFDKEYS
                     FNAEDIEPMITYGTNPGMGIAVSDKIPDNDDKGLEKALQYMGFKKGEKLTGKNIDYVF
                     LGSCTNSRMEDLRMFCDFVKGKQKAENVTVWIVPGSKQIEKQAIAEGLNKILQDAGME
                     LREPGCSACLGMNEDKIPAGKYCVSTSNRNFEGRQGQGARTLLASPLTAAVAAITGKI
                     DDIRNYL"
     gene            1999..2592
                     /gene="leuD"
                     /locus_tag="PLF48_07520"
     CDS             1999..2592
                     /gene="leuD"
                     /locus_tag="PLF48_07520"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNF49209.1"
                     /translation="MEKYTTIQSTCVPLNIEDVDTDQIIPARFLKATTREGFGDNLFR
                     DWRFNPDNTPKENFVLNDDTYVGNVLVTGKNFGCGSSREHAAWAIYDYGFRVVVSSFF
                     ADIFKNNALNNGLLTVVVTPEFLTLLFSEIEKNPLTQVNVNLTTQTISILDASLNPVA
                     TESFEINPYKKTCLLNGYDDIDYLLSLKNKIKAFELS"
     gene            2651..3436
                     /locus_tag="PLF48_07525"
     CDS             2651..3436
                     /locus_tag="PLF48_07525"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNF49210.1"
                     /translation="MNKSVQLNFQLTPDKKVYFLSDLHLGAQSYTQSLAREKTIISFL
                     NHIKTDAQVLFFVGDIFDFWFEYRQAVPKYFVRFLAKIAELKDAGIDIYFIKGNHDLW
                     MRNYLNDELGVQIIEDTIELNSGGKRILITHGDGKGKGDLKYKILKKIFTNPVSNFLF
                     SWLHPDIGIKIALLWSRSSFTDPKTEVFKGEENEWLLQYAQQMANEKQYDFVLMGHRH
                     LPMEINLANGATYINLGDWIYNFTYAVFDGNLLSLKKWDNEQQ"
     gene            3423..4238
                     /locus_tag="PLF48_07530"
     CDS             3423..4238
                     /locus_tag="PLF48_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49211.1"
                     /translation="MNSNSIFGHIKTGVTVFYMLSAIVLFAQSPQKLKTIPVKAYNVF
                     VNGTDQVYILSKGMLELHLLNGSPVLQYGSKYINKHTKIISVNSFKTILYSEDYGKII
                     TLDNRLKQQEIFDIYDLGSTVLVSCVGASLINNTIWIFDAATQKLSKLDENHKVLFTS
                     NSISQALGKEIHPEQIHEFGIYVYLVDEENGIYIFDTQGNYVKYIPIFNAHNFSVVSS
                     KFYYVKDNSAFEYNPLTFTETKFLKTPDLKYLHFGSSVLAGINKNNEVEIWTF"
     gene            complement(4240..4839)
                     /locus_tag="PLF48_07535"
     CDS             complement(4240..4839)
                     /locus_tag="PLF48_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF49212.1"
                     /translation="MKNLSLILNAILFALVGVLFFLHFKGDKKAATPVVVNANGKVVN
                     SGLIGYIDIDSFQDNYGYFKAKKAELEASQANMESELNRKVSAFQNEYAKLAQKAQTM
                     TEEEGMAAQEKLAKQQQDIEMRKAQMEEKFANQMADFNKTLENKIIEYLKKYNADGKY
                     AYILPYSRSQINLLYVTPTYDLTGEVLKGMNEEWSSNKK"
     gene            4997..5079
                     /locus_tag="PLF48_07540"
     tRNA            4997..5079
                     /locus_tag="PLF48_07540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5031..5033,aa:Leu,seq:tag)
     gene            5135..>6447
                     /gene="tig"
                     /locus_tag="PLF48_07545"
     CDS             5135..>6447
                     /gene="tig"
                     /locus_tag="PLF48_07545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNF49213.1"
                     /translation="MNISLNKTDELNAVISVDVKTEDYLPAVKKTLHQYKQTVPMKGF
                     RKGFVPEGLIKKMYGNGIMLDEINKLVTDGINKYIDEEKLDFLGRPLPKADADIHFDI
                     NSPQDFVFEYELGLTPEFTLKGLDKKNKIEKKVVVIDDKTLNDEMDNLARRYGNATHP
                     EDGIEDKDILTLELTELDGKKEKENGVTHTAVVGLDMFKDDKVKAKFLKAKLGDAITI
                     KPYEVFDNANDYVSKSILGLVDGNPEGLSDSFKATISKVTRLEKAAFNQELFDKIYGP
                     GVVESEEKFKERLKNELEDYAQSSTNNQLKKDIYDSLMEKNNIALPDAFLKRFIKVSN
                     EKPISDEQIEQEYPAFEKGLRWNLITAKIAKDNQIKVEHEEVSNFSKEQLKKQLAMYN
                     PTGAGIEDEYLDIFNNNMLAKEDHAKKSYEGAMEQKLFSFIENQIG"
BASE COUNT         2132 a         1178 c         1231 g         1906 t
ORIGIN      
        1 ccacaataaa tgcaaaaaat gcagcttaaa aactgttttt agacactttt aaacctacaa
       61 ttcctattct gcatcctact acaaatataa atgtcaatta aattaataat ctatataaag
      121 aatgttattt tgttccccaa aaaattgcat ttaacacttg aacttaggta taagcacttg
      181 tgtgtattgt tttataattt ttaccatttg aggcatagat tttttaggat taacaatgtt
      241 atccatacaa aaatcaggca agaataaaaa aatacaggaa tcgccaatgg cgatactatg
      301 tttttgcttg cctgtctata ctgttttgca ccaaacaaca atttatgatt agaaatacta
      361 tgattctatg tggtttaaac cgcacaacga attaatatta gtattagctt aatcaataaa
      421 ccaatcagca ttacgcactt ttttaacaaa cacataaact cgcttttctg ctataatatg
      481 tctgattgta taccttacat cttcatcttg ttttatggga aaaactttat ttgataaaat
      541 ctgggattcg cacattgtgg aagccataga aaacggacct caggtaattt atatcgacaa
      601 acattttatt cacgaagtaa ctacaccgca ggcttttgaa gggttgcgca aacgcagcat
      661 tcccgtattc cgtacacaaa ataccgttgc tacagccgac cataatgttc cgactaaaga
      721 ccaaaacctg cccattcggg aggctttatc caaacatcag gtagatacac tcattcaaaa
      781 ctgtactgat tttggcatcg aactttatgg actgggacat ccgtttcagg gcattgtaca
      841 tgtcattggt ccggaacttg gcatcacaca acccggcatg accatcgtat gcggtgattc
      901 acatacttcc acgcatggcg cgtttggagc ggtagcattt ggcatcggca cctcggaagt
      961 agaacaggta cttgccacgc aatgcatttt acaaacacgt cccaaaacca tgcgcattaa
     1021 tatcgacggc gagctgggca aaggcgttac cgcaaaagac atcactctct atattatttc
     1081 caaaatatct acaggtggcg gaacgggtta ttttgtagaa tatgccggaa gtgctatccg
     1141 cagcctgagc atggaagccc gtatgacagt ttgcaatatg agcatagaaa tgggtgcgcg
     1201 tggaggaatt attgcaccgg atgaaacaac ttttgcctat ttgaaaggaa ggacttttgc
     1261 ccctgaaaat tatgagcaag ctttggaaaa atggcaaacc ttatttacag atgaagatgc
     1321 tgcatttgat aaagaatatt catttaatgc tgaagatata gagcctatga ttacctacgg
     1381 aactaatccg ggaatgggca ttgccgtatc agataaaatt ccggataatg atgataaagg
     1441 attagagaaa gccttacaat atatgggctt taaaaaaggc gaaaaactaa cagggaaaaa
     1501 tattgactat gttttcttgg gaagttgtac caatagccgc atggaagact tacgtatgtt
     1561 ttgcgatttt gtaaaaggca aacaaaaagc agagaatgta actgtgtgga tagtacccgg
     1621 cagtaaacaa atagaaaaac aagctattgc cgaaggtttg aataaaattt tgcaggatgc
     1681 tggaatggaa ttgcgtgagc cgggatgttc cgcatgtttg ggtatgaatg aagataaaat
     1741 acctgccgga aaatattgtg tatctacatc caaccgaaat tttgaagggc gacaaggaca
     1801 aggtgcacga accttgcttg ccagtccgct tacggctgcc gtagctgcca ttacaggcaa
     1861 aattgacgac atcagaaatt acttatagca aagcttgttt atacctgaat gatgaagaat
     1921 aaatgacatg aaatactaac ttaccaatgt accaatgacc aatgtactaa tgactaatca
     1981 ctaataacca atcaataaat ggaaaaatat actacgatac aaagtacctg cgtgccactc
     2041 aatattgaag atgtggacac cgaccagatt attcctgcac gttttcttaa agccacaaca
     2101 cgtgagggtt tcggagataa tttatttcgc gactggcgtt ttaatccgga caatacaccc
     2161 aaagaaaatt ttgtcttgaa tgacgatact tatgtaggca atgtattggt aacaggtaaa
     2221 aattttggtt gcggttcatc gcgcgaacat gccgcctggg cgatttatga ttatggtttc
     2281 cgtgtggtcg tgtccagttt ttttgcagat atttttaaaa acaatgcact gaataatgga
     2341 cttctaactg tagtggttac gccggaattt ttgactttac tatttagtga aatagagaaa
     2401 aatccgctta cacaagtaaa tgtaaattta accacgcaaa ccatctccat tttagatgct
     2461 tcattgaatc ccgttgcaac tgaatccttt gagatcaatc catacaagaa aacttgttta
     2521 ctcaacggat acgatgatat cgattattta ttgagtttga aaaacaagat aaaggcgttt
     2581 gagttatcgt gaaaatcaca aggaattttc tgtacatttt tttagttagc attttccgta
     2641 tctttattca gtgaataagt ctgttcaatt aaacttccaa cttacacctg ataaaaaagt
     2701 ttatttttta tcggatttgc atctcggggc gcagtcttat acacagagtt tggctcgcga
     2761 aaaaacaatc atttcttttt taaatcatat caaaacagat gcacaagtcc tcttttttgt
     2821 tggcgatatt tttgattttt ggtttgaata ccgtcaagcc gtaccgaaat attttgtacg
     2881 ttttttggct aaaattgccg aactgaaaga tgccggcatt gacatctatt ttatcaaagg
     2941 caaccacgat ttgtggatgc gcaattacct gaatgatgag ctcggtgtgc aaataataga
     3001 agacactatc gaattaaata gtggtggaaa gagaatactc attacacacg gcgacggaaa
     3061 aggcaagggt gacttgaaat acaaaatatt aaagaagatt tttacaaatc cagtcagcaa
     3121 tttccttttt tcgtggctac atcccgatat tggtataaaa attgcgctgc tttggtcgcg
     3181 cagcagtttt accgacccca aaacggaagt atttaaaggc gaagaaaatg aatggctgtt
     3241 gcaatatgcc caacaaatgg ctaatgaaaa acaatacgat tttgtgctaa tgggacaccg
     3301 gcatttgccc atggaaatta acttggcaaa tggtgctacc tatatcaatt tgggcgactg
     3361 gatttacaac tttacttatg ccgtttttga cggcaatttg ctctcactca aaaaatggga
     3421 caatgaacag caatagcatt tttggacata tcaaaacagg agtaactgta ttttatatgc
     3481 tgtctgcaat tgtattgttt gcccaatcgc cgcaaaaact aaaaaccatt cccgtaaaag
     3541 cctataatgt atttgtaaac ggcactgatc aggtttatat tttgtcaaaa ggcatgctcg
     3601 aattacattt gttgaatggc agtccggtat tgcaatatgg cagcaaatac attaacaagc
     3661 atacaaaaat catcagtgta aacagcttta aaactatctt gtattctgaa gactatggca
     3721 aaattattac gctggacaac aggctgaaac agcaagaaat atttgatatt tatgatttgg
     3781 gttctacggt attggttagc tgtgtaggtg catcgttaat caataacacc atttggattt
     3841 tcgatgccgc cacgcaaaag ctgagcaagc tcgatgaaaa ccacaaagtg ctgtttacct
     3901 ccaattcgat atctcaggca ttaggtaaag aaattcatcc ggaacagatt cacgaatttg
     3961 gtatttatgt ttatctggtg gatgaagaaa acggcattta tattttcgac actcaaggca
     4021 attatgtcaa gtacattcct atatttaatg cgcataattt ttcggtggta agcagcaaat
     4081 tttattatgt aaaagacaat tccgctttcg agtataatcc gctgacattc accgaaacca
     4141 agtttttaaa aacacctgat ttaaaatatc tgcatttcgg ctcttctgtt ttagccggga
     4201 ttaataaaaa taatgaagta gaaatctgga cgttttagct tatttcttat tgcttgacca
     4261 ctcttcgttc atgcctttta gtacttcgcc agtcaaatcg taggttggcg taacatacag
     4321 caaattgatt tgcgatctgg agtagggcaa aatataagca tattttccgt cggcattata
     4381 tttcttgagg tattctataa tcttattctc cagcgtctta ttgaaatcag ccatttgatt
     4441 ggcaaatttt tcttccatct gcgctttgcg catctcaata tcttgttgtt gttttgccaa
     4501 tttttcctgt gcggccatcc cttcttcttc ggtcatcgtt tgagcctttt gcgctaattt
     4561 ggcgtattca ttctgaaaag cacttacttt tcggttcaac tcgctttcca tattggcttg
     4621 agaagcttcc agttcagctt ttttggcttt aaagtagcca taattatctt gaaaagaatc
     4681 aatatcaata tagccaatca atccgctgtt gactaccttg ccattagcat ttaccactac
     4741 cggagttgcc gcctttttat cgcctttaaa atgaagaaaa aacaatacgc ctaccaatgc
     4801 aaacaatatg gcgttcaata tcaaggataa atttttcatg gcgcaaagat agttcagttt
     4861 tcggctgatt ttactcaaaa ttcaaaatat actttactat tttgtaaaaa ggcgttcatt
     4921 attaaccatt tttaatttaa tttttttgcg tttgactaaa tgttcgtatc tttgcagtcc
     4981 aatttttatt ggaaatgcgg gtgtggcgaa attggtagac gcactagact taggatctag
     5041 cgggcaacca tgggggttcg agtccctcca cccgcaccac aaaacgccac gacaaagtcg
     5101 tggtttttta tttaaacaaa caaattaaca tatcgtgaat atctccttaa acaaaaccga
     5161 tgaactaaac gccgtaatca gcgtggatgt aaaaactgaa gattatcttc ctgccgtaaa
     5221 aaaaacattg caccagtaca aacaaaccgt accgatgaaa ggtttccgca agggatttgt
     5281 tcccgaaggg ctgattaaaa agatgtacgg caatggcatt atgctggatg aaatcaacaa
     5341 gttggtaaca gacggcatca ataaatatat tgacgaagaa aaactggatt ttctgggcag
     5401 acctttaccg aaagctgatg cagacattca ttttgacatc aactcaccac aagattttgt
     5461 ttttgaatac gagttgggct taacgcctga atttacttta aaaggcttgg ataaaaaaaa
     5521 taaaatagag aagaaagtag ttgttattga tgataaaaca ttaaatgatg agatggataa
     5581 tttagctcgt cgctatggca atgctacaca tcctgaagat ggtattgagg acaaggatat
     5641 tttaactctg gaactgacag aactggatgg caaaaaagag aaagaaaacg gagtaacaca
     5701 tacggcagta gtaggcttag atatgtttaa agacgataag gtgaaagcaa aatttctgaa
     5761 ggctaagctt ggcgatgcca ttaccataaa gccttatgaa gtctttgata atgcaaatga
     5821 ctatgtaagc aaaagtattt tagggttagt tgacggtaac cccgaaggtc tttcagattc
     5881 atttaaagcc actatctcta aagtaacaag actggaaaaa gcggcattca atcaagaatt
     5941 gtttgataaa atatacggcc cgggagttgt tgaatctgaa gaaaaattca aagaaagact
     6001 taaaaatgaa ctggaagact acgcccagtc atctaccaac aaccaactga aaaaagatat
     6061 ttatgacagt ttgatggaaa agaacaacat cgcactgccg gatgctttct taaaacgatt
     6121 tattaaagta agcaatgaaa aacccatctc tgacgaacaa attgaacagg aatatcctgc
     6181 ttttgaaaaa ggattgagat ggaatttgat tactgcaaag attgctaaag acaatcaaat
     6241 taaagtagaa catgaagaag tatccaactt cagcaaggaa caactcaaga aacaattagc
     6301 catgtataac cctacaggtg ccggcattga agatgagtat ctggatatct ttaataataa
     6361 tatgctggct aaggaagacc atgccaaaaa gtcatacgag ggtgcaatgg aacaaaaatt
     6421 gtttagcttt atcgaaaatc aaatcgg
//
LOCUS       DAOQII010000138         6407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6798_length_6407_cov_4.745907, whole genome shotgun sequence.
ACCESSION   DAOQII010000138 DAOQII010000000
VERSION     DAOQII010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6798_length_6407_cov_4.745907"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLF48_07550"
     CDS             <1..346
                     /locus_tag="PLF48_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNF49214.1"
                     /translation="NNAALAVVCSGTATLQTALLNTPQVVVYRTSFINYQIGKRLAKV
                     NYISLPNLILNREIIKELLQQDCTAKNILTEIKKIEAGFNFKAYAQLRNILQTGNAAE
                     NAATEILKTINK"
     gene            complement(347..1117)
                     /locus_tag="PLF48_07555"
     CDS             complement(347..1117)
                     /locus_tag="PLF48_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF49215.1"
                     /translation="MFSNDSFKDKIVLVTGGGSGIGFTIAKQFLMHGAIVFISGRNAE
                     KLQKAINELEKYGTVHSMIADIREPEQIEKLADAIKSAYGKLNILINNAGGQFPSAAE
                     NISYNGFRAVINNNLIGTFYVAQIMAKSFFIPQKGGNMVNIIANVFRGFPGMSHTGAA
                     RAGVDNLTKTLAVEWARYAIRVNAVAPGIIKSTGLENYPPDFLKNIEAQIPAKRMGTT
                     EEVAYPVLFLASDMATFITGETLYVDGGNRLWGDQWKM"
     gene            1252..1605
                     /locus_tag="PLF48_07560"
     CDS             1252..1605
                     /locus_tag="PLF48_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49216.1"
                     /translation="MRQLLITLLVSSVILFSCKKKNTLDGVNFNNTHWNLFYKNGPTF
                     TFYAKTHLFLKDNLDFENYGNVDTTYGTWRTDDHLYLKFNNGAYYTSDFISSDSLHGT
                     IFVNGIYGNWYATRR"
     gene            1709..2296
                     /locus_tag="PLF48_07565"
     CDS             1709..2296
                     /locus_tag="PLF48_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNF49217.1"
                     /translation="MTTACTLDTSKSCLLVRENPNDRWQIFEDSITGFTHKKGTQYCL
                     LVKVVDDKTKNKQRYVLEEIKSTSLTDTLAEIQKPQVTNSIFCGKKWMLYKLKTKNEL
                     KIFTIAKAILSFDLSSTHLKLTLDCAQYAGEFLSQNESLDFKNLNAITSTCKKRSVED
                     DLLKMLSLTNRYKIRKKELLLFKDDILLSVWLLKK"
     gene            complement(2352..2642)
                     /locus_tag="PLF48_07570"
     CDS             complement(2352..2642)
                     /locus_tag="PLF48_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49218.1"
                     /translation="MKQYSSTTVTPETSDKLLFSLHNEDFFFHCWYSLIENRFIVIKE
                     PRHMDCTIKNQIYNFNSKEIKQYLIHQTNTLKNSELLFYNEQIWNELSGQLN"
     gene            2766..3671
                     /locus_tag="PLF48_07575"
     CDS             2766..3671
                     /locus_tag="PLF48_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNF49219.1"
                     /translation="MSQKTALRRYNIIINQLRKKPSTYIDFCNQLKQESEIDGYDYVI
                     SKRTLKRDIEDILYLYGIEIRFDFSRQVYAIRDTADNTANLKVLEAFDIINAFHVNAD
                     NEQYLHFDKRTPQGTQHLLPILDAIRKRKCIQILHQKFAADQPSSRLLEPLALKEFQY
                     RWYLVANDTKDGKIKSFGLDRMIHVEMTNKKYTYPSNFDANTFFKYSFGIVSGRKNVK
                     PAKIVLSFSINLKPYLMTLPLHDSQKLIKETGTDFLIELELHPTQDLIREIASYGNQV
                     QVIKPESFRKAVFHYVRPEPVILQP"
     gene            complement(3655..>6407)
                     /locus_tag="PLF48_07580"
     CDS             complement(3655..>6407)
                     /locus_tag="PLF48_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF49220.1"
                     /translation="TGASISVSPTTTTTYTVTGTTNGCDGTATSTVTVKSNPTVTVNS
                     PSICNSQSATLTASGATTYSWNTGATGATISVSPTTTTTYTVTGTTNGCDGTATSTVT
                     VKPKPVVTVNSDNICPGQSSTLTANGATTYAWNTGATSPSITVSPGSTTTYSVTGTTN
                     GCSANATATVNVASSLPVIVNSPSICNGQNATLTASGATTYTWSTGVSGATISVNPTS
                     TTTYTVTGSTNGCSGTATSTVTVKPNPTVSVNSTSICNGQSATLTAIGATTYSWNTGV
                     TGTSISVNPTSTTTYTVTGTTNGCDGTATSTVTVKPNPTISVNSTSICNGQSATLTAS
                     GATTYSWNTGATGATISVSPTITTTYTVTGTTNGCDGTATSTVTVKPNPTVTVNSPSI
                     CNGQSATITASGATTYSWNNGSTGATISVSPTTTTAYTVTGTTNGCQGTATSTVTVKA
                     NPVVTVNSPTICNGQTVILTASGATTYSWNTGASSASISVSPTVATTYTVTGTTNLCN
                     DTKQAVVTVYNTPVTNLVKSICSDDSFKVGNQYFNTAGNYTVHLQSSLNCDSTVNLNL
                     TVVPSLTPTVSITANKTDICPNEQVVFTSNVNNTRSNITYQWFVNNILSGTNSQFISN
                     QLHNGDVIHLVIHSPGNCMSVDSAVSNSIVIHASSILFTVPTVAYCPDDKDTIDLSVQ
                     YTDAVHPDYSIIWSSGTNVDTVINSATYPVDAQTASPVRFTLLYGNQCRVSGNVPLQV
                     LARPFVDATTNHNLVSYQTEVQLNATGNNLTSFNWLPSSAVSNAQIQNPTSVIQASTW
                     FYVTVKDNKGCINSDSVFVEMKNECHESDLYIPSAFSPNNDGVNDCFGLLQPPVLSNY
                     SLTIYNRWGELLFESHDVNNCWNGKYKGSVVENDSYIYIVRYICYDGTPLSKKGTITV
                     VK"
BASE COUNT         2029 a         1160 c         1182 g         2036 t
ORIGIN      
        1 aaataatgct gcattggctg ttgtttgcag tggcactgct accttacaaa cggccttgct
       61 gaatactcct caagtggtag tttaccgcac ttcgtttatc aattatcaga ttggcaaaag
      121 attggcaaag gtgaattata tttccttacc taatttaatc ctaaaccgag aaatcataaa
      181 agaattactc caacaagatt gtactgcaaa aaacattttg acagaaataa agaagataga
      241 agcaggtttt aattttaaag cctatgccca gttgagaaat atactacaga caggcaatgc
      301 cgctgaaaat gccgctacag aaattctaaa aacgataaat aagtaattac atcttccact
      361 gatctcccca aagtctgttt cctccatcca catagagcgt ctcaccagtg ataaacgtag
      421 ccatatctga agccaaaaac aaaaccggat aagcaacttc ttccgtcgta cccatgcgtt
      481 tcgccggaat ttgagcttca atatttttca aaaagtcagg cggataattc tctaatccgg
      541 ttgatttaat gatgccggga gcaacagcat ttacccgaat agcatagcgt gcccactcaa
      601 cagctaaagt ctttgtcaaa ttatccacac ccgcacgtgc agctcccgta tgcgacatac
      661 ccggaaaacc tctgaaaaca ttggcaatga tattgaccat attgcctcct ttttgcggaa
      721 tgaaaaatga tttggccata atctgagcaa cataaaatgt tccgattaag ttattgttta
      781 tcaccgccct gaaaccatta tagctgatat tctcagctgc tgacggaaat tgaccaccgg
      841 cattattgat taagatattg agttttccgt atgccgattt aatagcatct gctaattttt
      901 ctatctgttc cggttcacgg atatcagcaa tcatgctgtg cactgtgcca tatttttcca
      961 gttcattaat cgccttttgc aatttttctg catttctgcc ggaaataaat actatagctc
     1021 catgcattaa aaattgtttg gcaattgtaa atcctatacc actgccgcca cctgtaacca
     1081 atacgatttt atccttaaag gagtcattag aaaacataca ttgaattttg ataaaggtat
     1141 aaatttaatg acacaataaa aaaaagaacg attgaacaat ttacttcttt atgagatgtc
     1201 aagtgcatca aatgatttta ttttataact ttgtttaaac acgtttgcaa tatgagacaa
     1261 ttgcttatta cgctattagt tagttctgtt attctgtttt cctgtaaaaa gaaaaataca
     1321 ctggatggtg tgaactttaa caatacccat tggaatttat tttataaaaa cggaccaaca
     1381 tttacctttt acgcaaaaac acatttgttt ttaaaagaca atcttgattt tgaaaattat
     1441 ggcaatgtag atactactta cggtacttgg cgtactgatg atcatttgta tctgaagttt
     1501 aacaatggtg catactacac cagtgatttt atctcatctg acagcctgca tggaacaatt
     1561 tttgtgaatg gtatatatgg caactggtat gctacacgca gatagaagta tgtatataat
     1621 caagcataca attttttcat ttttcatcct gttattttcc tgtaatataa gggcgcaatc
     1681 tttctataag ataatctatg tttcagattt gacaactgcg tgtacgttag atacctctaa
     1741 atcttgtttg ctggtaagag aaaatccgaa tgacaggtgg caaatttttg aagacagtat
     1801 cacaggattt acacacaaaa aagggacaca atattgttta ttggtaaaag tagttgatga
     1861 taaaaccaaa aataaacagc gctatgtttt agaagaaata aaatcaacat ctttgactga
     1921 tacgttggcg gaaatacaaa agccacaagt gacaaatagc atcttctgtg gaaagaaatg
     1981 gatgttgtac aagttaaaaa caaaaaacga actgaaaata tttaccatag caaaagcgat
     2041 tttgtctttt gatttgagca gtactcattt gaaactgacg ttagattgtg cacagtatgc
     2101 gggggaattt ttatcccaaa atgaaagtct tgatttcaaa aatttaaatg cgattacatc
     2161 cacttgcaaa aagcgaagcg tagaagacga tttgctaaaa atgctctcac tcaccaaccg
     2221 ttataaaatc agaaaaaaag aactgctact gtttaaagat gatattcttt tatctgtttg
     2281 gttgctgaaa aaatagcata ttctgtcact caaaataatg acagaatatg caccaaacta
     2341 caaagtataa attagttcaa ttgtccgctt aactcattcc agatttgctc attataaaac
     2401 aacagttctg agtttttaag tgtattcgtt tgatgaatga gatattgttt gatttctttg
     2461 gaattaaaat tataaatttg atttttgatg gtacaatcca tatgacgcgg ctctttgatc
     2521 acgataaacc gattttcaat caagctatac cagcaatgaa aaaagaaatc ttcattgtgc
     2581 aacgaaaaca aaagtttgtc actggtttca ggggttactg ttgtggaaga atactgtttc
     2641 atttttttaa ttttatgcgt tataaaataa ttaatgggtc aaaattatct tcagactaag
     2701 ccaacctatg tcttagtcta atttttttat gttaataaat tgttaagttt ttaaaacaga
     2761 ttaaaatgtc tcaaaaaaca gcgctgcgca gatataatat tatcatcaat cagttacgga
     2821 aaaaaccttc tacgtatatt gacttttgta atcagctaaa acaagaatcg gagattgacg
     2881 gctatgatta tgtaatcagc aaacgcacgt tgaaaagaga tatagaagat atactttatt
     2941 tatatggaat tgagattcgt ttcgattttt cgagacaagt gtatgccatt cgggatactg
     3001 cagataatac cgctaacttg aaagtactgg aagcattcga tatcattaat gcgtttcatg
     3061 tcaatgctga caatgagcag tatctgcatt tcgacaaacg caccccacag ggtacacagc
     3121 atttattacc tattcttgat gctattcgta agcggaaatg tattcaaatt ctacatcaaa
     3181 aatttgcagc tgaccaaccc tcctctcgtc tgttggaacc actcgcacta aaagaatttc
     3241 aatacagatg gtatttagtc gcaaatgaca caaaagatgg aaaaatcaaa tctttcggat
     3301 tagacagaat gattcacgta gaaatgacaa ataaaaaata cacatatccc agtaactttg
     3361 acgcaaacac atttttcaaa tattcgttcg gtatagtttc aggtaggaaa aatgtcaaac
     3421 ctgctaaaat tgtattatcc ttctccataa atttaaagcc ctatctgatg acattaccgc
     3481 tgcatgattc gcagaaactc attaaagaaa ctggtacaga ttttctcatt gagctggaat
     3541 tacatccaac tcaagatttg atccgagaaa tagcatccta tggaaatcaa gttcaggtca
     3601 taaaacctga atcatttaga aaagctgtat tccactatgt acgacctgag cctgttattt
     3661 tacaaccgta attgttcctt ttttagatag aggtgttccg tcataacaaa tatatctgac
     3721 aatatagata taagaatcat tttcaactac actgccttta tacttgccat tccagcagtt
     3781 atttacatca tgtgattcaa acaataactc tccccatctg ttataaattg tcaaactata
     3841 attgcttagt accggcggtt gtaacaagcc gaaacaatcg ttaacaccat cattatttgg
     3901 tgagaatgcg gaaggaatat acaaatcact ttcatggcac tcgtttttca tttctacaaa
     3961 aacagagtct gaattgatac atcctttatt gtcttttacg gttacataaa accaagtaga
     4021 agcttggata acagaagtcg gattttgaat ttgagcattg ctgacagcac tcgaaggaag
     4081 ccagttaaat gacgtcaaat tattgccggt tgcattcagt tgtacttccg tctgatagct
     4141 gaccaaatta tgatttgtgg tggcatctac aaaaggacgg gcaagcactt gtaatggcac
     4201 gtttcctgac accctgcatt gattgccata aagcaaagta aaccttaccg gagatgcagt
     4261 ttgagcatca acgggataag tagctgaatt gattacagta tcgacattag taccgctact
     4321 ccatataata ctataatccg gatgaacagc atctgtatat tgaaccgata aatcaatcgt
     4381 atctttatca tcaggacaat atgctacagt cggaacagta aacaaaatac tgctggcatg
     4441 tattactatc gaattactta ctgctgaatc aacagacatg caattacccg gtgaatgaat
     4501 gactaagtga ataacatctc cattatgtaa ttggttggaa ataaactgac tgttcgtgcc
     4561 ggataaaata ttatttacaa accattgata ggtgatatta cttcgggtat tatttacgtt
     4621 tgacgtaaat acaacttgct catttggaca aatatctgtt ttgttagcgg taatacttac
     4681 tgtcggtgtt aatgaaggaa caacagttaa gtttaaattt acggtactgt cgcaattaag
     4741 tgaagattgt aaatgaacgg tataatttcc tgcagtatta aaatattgat tgcctacttt
     4801 aaagctgtca tcagaacaaa tagatttgac caaattcgta acaggtgtgt tatatactgt
     4861 tacaacagcc tgttttgtat cattacataa attagttgtt cctgttacgg tataggttgt
     4921 tgcaacagtt ggactaacag aaatactcgc actggatgca cctgtattcc aactataagt
     4981 agtagctcca ctagcagtta atataactgt ctgaccatta caaatggtag gactatttac
     5041 agttacgact ggatttgctt ttaccgttac ggtggatgtt gctgttccct gacaaccatt
     5101 tgttgttccg gttacggtat aagctgtcgt agtggttgga ctaacagaaa tagtcgcacc
     5161 tgttgaaccg ttattccagc tataggtagt tgcaccactt gctgtaattg tggctgattg
     5221 accattacag atagaaggac tgtttaccgt taccgttgga ttgggtttta cggttacggt
     5281 ggatgttgca gttccatcac aaccattagt cgtgcctgtt acggtgtaag tagtagtaat
     5341 tgttggacta acagaaatag ttgcaccagt tgctcctgta ttccaactat aagtagttgc
     5401 tccacttgcc gttaatgtgg ctgattgacc attacaaata gaagttgaat ttacacttat
     5461 agttggattg ggtttcaccg ttacggtgga tgttgcagtt ccatcacaac cgtttgttgt
     5521 tccggttacg gtataagttg tggttgaagt aggattaaca gaaatacttg tacctgttac
     5581 tcctgtattc cagctatagg tagtcgctcc aattgcagtt aatgtggcag attgaccatt
     5641 acatattgaa gttgaattta cactaactgt tggatttggt tttactgtta ctgtggatgt
     5701 tgcagtacca gaacatccgt tagtcgatcc ggttacagta taagttgttg ttgaagtagg
     5761 gtttacggat attgttgcgc cggaaactcc tgtactccaa gtatatgttg tggcaccact
     5821 tgctgttaaa gttgcatttt gtccgttaca gatagatgga ctattgacga taacaggtaa
     5881 cgaagatgca acattcacgg tggcagttgc atttgcggaa caaccatttg ttgttccggt
     5941 aaccgaataa gtagtagtag aacctggtga gacagtaata cttgggctcg tagcaccagt
     6001 gttccatgca tatgtagtag caccatttgc tgtaagcgta gaactttgtc ccggacaaat
     6061 attatcacta ttcactgtaa caacaggctt aggttttact gttacggttg aggtggctgt
     6121 tccatcacat ccgtttgtcg tgcctgttac ggtataagtt gtcgtagtag ttggactaac
     6181 agaaatagtt gcacctgttg caccggtatt ccagctatag gtagtcgctc cacttgctgt
     6241 aagtgtggct gattgactat tacaaattga agggctgttt actgttaccg ttggatttga
     6301 ttttactgtt actgttgatg tggctgttcc atcacaacca ttagttgttc cggttacggt
     6361 ataagttgtc gttgtggttg gactaacaga aatacttgca ccagttg
//
LOCUS       DAOQII010000139         6367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_6865_length_6367_cov_5.127693, whole genome shotgun sequence.
ACCESSION   DAOQII010000139 DAOQII010000000
VERSION     DAOQII010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6865_length_6367_cov_5.127693"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1717
                     /locus_tag="PLF48_07585"
     CDS             74..1717
                     /locus_tag="PLF48_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF49221.1"
                     /translation="MKKLLAIFLICLVLNARAGDKDRYFEIAKNMEIFSEVYKELNNT
                     YVDDVDPNKLMTDGIVKMLDGLDPFTNYFTGSDLDEYQLQVTGKYAGIGSRIMKQGDY
                     VVISEPYEGFPAQKAGLQAGDAILEIDGKSVAKYNSDEISQLLKGEPGTKISIKVKRA
                     VTNEEKTFTFNREEIKIDNVPYFGMLNKNIGYIRLSSFTDNASTEVQNALKELKKNPE
                     FKGLVFDLRGNGGGLLNEAINICNVFVDKGTAIVSTKGKIADANNNYTTQNKPLDLDV
                     PLVFLTDGGTASASEIVSGSMQDLDRGIVIGQNTYGKGLVQITKPLPYKGKLKVTVSK
                     YYIPSGRCIQRIDYSHRSDNGIASAVPDSLRKEFKTKAGRTVKDGAGIEPDIKTTLQE
                     TNPITIALYTNNHFFNYANVYKSKHTDLRDGNKFRLTDDEYNDFVNYLKDKTYTYTNE
                     AEAKLNDLQKSAEESHLSENSMKLLTELGDKIKEDKSKDLITHKTEIKELLEEEIVSR
                     YTYEKGRIANSLKDDKDVLKAIEILSDNNTYKQILTAKK"
     gene            complement(1943..2233)
                     /locus_tag="PLF48_07590"
     CDS             complement(1943..2233)
                     /locus_tag="PLF48_07590"
                     /inference="COORDINATES: protein motif:HMM:NF026967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HNF49222.1"
                     /translation="MNKLIFLLVFALIFPLLVIAKESLTDQELIKIAYKAIKRRYNVI
                     HSSKYKPYVIQERTDTTLTISGTWNMPANCRGGTPFVIIRMDGKIIKISHGK"
     gene            complement(2316..5630)
                     /locus_tag="PLF48_07595"
     CDS             complement(2316..5630)
                     /locus_tag="PLF48_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49223.1"
                     /translation="MENNYQLLIERLDGFIRKYYLNKLLRGILLFLALVTVYYLFASV
                     FEYKLYFSSSVRKFILFSFMAVFIFALAYWVLLPLFHYLNLGNVITHETAATIIGNHF
                     QPVKDKLLNILQLKTQSQNAYNRDLIEASINQKMDGIKLVPFTQAVNLSENKKYLKYV
                     LPPLLAIAMILLIAPQIFKESNKRLANPDVIFAKKAPFDFVLENKKLKAIQYQDYEVA
                     LSIKGETLPNEVYINDGGKNYKMEKTAPDKFKYRYTNLQKDVHFYFSAGEYNSDEHTL
                     RILKKPVIANFSTQLTYPGYTKKPNETIKNTGDLVVPAGTTINWTFETSGTDKVNIQI
                     DGQSYAAQKESDDRYTFTKRILKDTRYTLYVSNNEVENGDSVSFAISATPDNLPAISV
                     ERIQDTLNKDFALFLGAVSDDYGLSRLEFHSALSDDKGKIISAKKQALPLANSSISDF
                     NFPLDFEKYGVKPGYKLNYYFEVWDNDAVNGPKSAKSQTYLYEKPTITQLEKQEFQNQ
                     EAIKDDLSSAMSKAQKLAADIKEMKKRILSKQNLNWEDKKQLEQIQKEHQELLQELKE
                     MEKKYDENLKNQDEYKKVDEEIMQKEEKIQEMMQDLMNDEMKDLMKQIEEILQKMEQR
                     NTFENLDKMQMSNENLKNELDKMQDLFKQLQLEQKAQETIDKLDQLAKDQEQLSNQTE
                     QNQNNSDQLKPKQEELNKKMDNIKENLQQLDKINKENEEKLNTDGNEEMSEDIEQDMD
                     KASDELGKNEKQKAGKSQKSASQKMKQMAQKMKNDLNKMQMSNTAEDIQTIRQLLENL
                     VKLSFDQEQLMNELKKTDIQTPKYVSIMQKQYDLRDDAKMIGDSLQSLGKRQFQLQQF
                     ISDELYKLNREMKKSLDNLEQRFKPQAGVAQQMVMTSANNLALMLSESLDNLQQQLQQ
                     QQQEGSGSCDKPGGKGKKSGGKPKMSLGEMQKQMNEQLKKMQDGMQNGKSGKQMGKDF
                     ADAVEKQAAIREALRKMKEKMSQQNKNGVDDVLNKMEENEKELMMKKLTPETIKRQKE
                     IETRLLEYEKAEREQGEEEKRQSKTAQEVPRKLPPALEEFLQKHKSAMEMYKTVPPNL
                     KPFYKELVEKYFRSVQ"
     gene            complement(5720..>6367)
                     /locus_tag="PLF48_07600"
     CDS             complement(5720..>6367)
                     /locus_tag="PLF48_07600"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF49224.1"
                     /translation="HLRFIKPFHELWIPDIKGVHNLSGKLAHGITFPIPHHYIGVMSR
                     FTNKIKHSSYIDDIDFEILIVLSGPEPQRSEMEAKLIKICNQQKKKTLLVRGKPDDEG
                     MKVKEFVTLIPLLQTNDLLAAYHKAAAIICRSGYSTIMDLSVLNKKAIYIPTHGQTEQ
                     EYLAHLMQERKMGVVWQPDELPLPDILKQYYTLKTNKQDTRYKNAFHKFISQYHF"
BASE COUNT         1763 a         1221 c         1173 g         2210 t
ORIGIN      
        1 ttttaaccat tcgtctaaac ttatttcatt agttttagtg tttaatactt taaaaaagat
       61 aacaaaaata agcatgaaaa aacttttagc cattttcctg atttgcttag tgctgaatgc
      121 gcgcgccgga gataaagacc gttattttga aattgccaaa aatatggaga ttttttccga
      181 ggtgtataaa gaactgaata atacctatgt tgatgatgta gatccgaata aattaatgac
      241 tgacggaatt gtaaaaatgt tggatggtct tgatccgttt actaattatt ttaccggatc
      301 tgatttagac gaataccagc tgcaggtaac cggaaaatac gccggtatag gatctcgtat
      361 tatgaaacaa ggtgattatg tggtaatcag cgagccctat gagggttttc ccgctcaaaa
      421 agcaggactg caagcgggtg atgccatctt agaaatagat gggaagtctg tagctaaata
      481 caattctgat gaaatcagcc agttgcttaa aggtgagcca ggcacaaaaa tctcgattaa
      541 ggtaaaacgt gcggttacga atgaagaaaa aactttcact tttaatagag aagaaattaa
      601 aatagacaat gttccctatt ttggtatgct caataaaaac ataggctata tccgtttgag
      661 ctcatttacc gataatgctt ctacagaagt acaaaatgcc cttaaagaac ttaagaaaaa
      721 tccggagttt aaaggtctgg ttttcgattt acgcggaaac ggaggaggct tgctgaatga
      781 agccattaat atctgcaatg tgtttgtaga taaggggact gctattgtga gtacaaaagg
      841 aaaaattgca gatgccaata ataattatac tacacaaaac aaaccccttg atttggatgt
      901 tccattggtg tttcttacag atggaggaac ggcatctgct tcagaaattg tttcgggctc
      961 tatgcaagat ttagaccgtg gtatagttat tggtcaaaat acttacggca aaggtttagt
     1021 tcaaattacc aaaccacttc catataaagg gaaattgaaa gtaacggtgt caaaatatta
     1081 tattcccagc ggaagatgta ttcagcgcat tgattattcg cacagaagtg ataatggaat
     1141 tgcatctgcc gttcccgatt cgctgcgtaa ggaatttaaa accaaagcag gcagaactgt
     1201 gaaagacggt gccggcatag aaccggacat caaaacaaca cttcaagaaa ccaaccccat
     1261 tactattgcc ttatatacca ataaccactt ctttaattat gccaatgttt ataaatcgaa
     1321 acacactgac ttaagagatg gtaataaatt caggctgaca gatgatgaat acaacgactt
     1381 tgtaaattac ctgaaagaca aaacttatac ttataccaat gaagcagaag ccaaacttaa
     1441 tgatttacaa aaatctgcag aggagagtca tttgtcagaa aactctatga aactcttaac
     1501 agaactggga gataaaataa aagaggataa atcaaaagat ttgattaccc ataaaaccga
     1561 aattaaagaa ctgttggaag aagagatcgt gagcagatac acgtacgaaa aagggagaat
     1621 agccaatagc ctgaaagacg ataaagacgt tttgaaagct attgaaatac tatccgacaa
     1681 taatacctat aagcaaatac tgacagctaa gaaatagcaa atcgttatat tttctaaaaa
     1741 agaaaacata tttattgatt tgtagaacat tttttaaaaa taattatata taatatgttc
     1801 aggacaaggt tgttttttta cagcctcaaa atcaactatt ttttaattga aatgcttatc
     1861 ccgaactcag agtagtaagt tttttaaata agcatattgc tccttatgca tatgggggca
     1921 gtatgcttat ttttttgttc tgtcatttgc catggctgat ttttattatt ttaccatcca
     1981 tgcggataat tacaaatggt gtaccgcctc tgcaatttgc aggcatattc catgtgccgc
     2041 ttatagtaag ggtggtatct gttctttctt gtattacata aggtttgtac ttcgacgaat
     2101 gaattacatt ataccttctt ttgattgctt tataggctat ttttatcaat tcttggtctg
     2161 tcaggctttc tttagcaatt actaataatg gaaatataag tgcaaacacg agtaaaaaaa
     2221 ttagtttatt catcttgttt ggtttcattt tttagagggt cagaaaataa acttctaaat
     2281 ccagatattg catatcttct tcttaccaga acaggctact gtaccgacct gaaatatttc
     2341 tccaccaatt ctttgtagaa gggtttcaaa ttgggtggca cggttttgta catttccata
     2401 gccgatttat gcttttgcaa aaattcttcc agtgcaggtg gcagttttcg tggcacttct
     2461 tgcgccgttt tagactgacg tttctcttct tcgccttgtt cgcgttcggc tttttcgtat
     2521 tctaataatc gggtttctat ttctttctgg cgttttatgg tttctggcgt cagctttttc
     2581 atcatcagtt ctttctcatt ttcttccatc ttgttcaata catcatctac cccgttttta
     2641 ttctgctgac tcatcttttc tttcatcttg cgcaaagcct cgcgtatggc ggcttgcttt
     2701 tctactgcat cggcaaagtc ttttcccatt tgtttcccgc tcttgccatt ttgcatgccg
     2761 tcttgcattt tcttcaactg ctcgttcatt tgtttttgca tttctcctaa actcatctta
     2821 ggcttacctc cgcttttctt tcctttaccc cccggcttat cgcagctgcc acttccttcc
     2881 tgctgttgtt gttgcagttg ctgttgcaga ttatccaacg attcgctcag cataagtgcc
     2941 agattattgg cagaggtcat caccatttgc tgcgccaccc cggcttgcgg tttaaatcgc
     3001 tgctccagat tatccaacga ttttttcatt tcgcggttga gcttatagag ctcatctgaa
     3061 ataaattgct gtaactgaaa ttgtcgcttg cccaaagatt gcagactgtc gcctatcatt
     3121 ttggcatcat cgcgcaagtc atattgtttc tgcataatgg aaacatattt cggcgtctga
     3181 atatctgtct ttttgagctc attcattaat tgttcctgat cgaaagaaag cttaactaaa
     3241 ttttccaaca actggcgtat ggtttgaatg tcttcggcag tattgctcat ctgcatttta
     3301 ttcaaatcat tcttcatctt ttgtgccatc tgcttcattt tctgcgaagc actcttttgc
     3361 gattttccag ctttttgttt ttcattttta cccaactcat ccgaggcttt gtccatatct
     3421 tgctctatat cttcgctcat ctcttcattg ccgtcggtat tcagcttctc ttcattttct
     3481 ttatttattt tatcaagctg ttgtaagttc tctttaatgt tgtccatttt tttgttcagc
     3541 tcctcttgtt ttggtttgag ctgatcggag ttgttttggt tttgttccgt ctgattgctt
     3601 aattgctcct gatcttttgc caattggtct aatttatcaa tcgtttcctg tgccttttgt
     3661 tccaactgca attgcttgaa taaatcctgc attttatcca actcattttt cagattctca
     3721 ttgctcattt gcatcttatc caaattctca aacgtgttgc gttgttccat cttttgcaaa
     3781 atctcttcaa tttgtttcat caggtctttc atctcgtcat tcatcaaatc ttgcatcatc
     3841 tcttgtattt tctcctcttt ctgcatgatt tcttcgtcca cttttttata ctcatcctgg
     3901 tttttcagat tttcatcata cttcttttcc atttctttca gttcttgcag caattcctga
     3961 tgttcttttt gtatctgctc caattgcttt ttgtcttccc agtttaaatt ttgtttgctc
     4021 agaatgcgtt tcttcatttc tttgatgtcg gcggctaatt tctgggcttt gctcatggct
     4081 gagctcaaat cgtctttaat ggcttcctga ttttggaatt cttgtttttc aagttgagta
     4141 attgttggct tttcgtagag ataagtttgc gatttggcag acttaggtcc gttcacggca
     4201 tcattatccc aaacctcaaa atagtagttt aatttataac cgggttttac gccatatttt
     4261 tcaaaatcta aaggaaaatt aaaatcagag atagaagaat tggcaagtgg caatgcttgt
     4321 tttttggcag aaattatttt gcccttatca tctgataagg cactatgaaa ttccaaacga
     4381 gataagccat aatcatctga aactgcaccc aaaaacaagg cgaaatcttt gtttagtgta
     4441 tcttgtatgc gctctacgct aatagccggc agattgtcgg gcgtggcaga gatggcgaat
     4501 gaaaccgagt cgccattttc cacctcatta ttcgacacat acaacgtgta tcgagtgtct
     4561 ttcaaaatac gttttgtaaa ggtgtatctg tcatcgcttt ctttttgtgc ggcatacgac
     4621 tgtccgtcta tttgtatatt cactttatct gttcctgaag tttcaaaggt ccagttaatc
     4681 gttgttccgg caggcaccac caaatcgccg gtgttcttga ttgtttcatt tggttttttg
     4741 gtatagcccg gataagtaag ttgtgtgctg aaattggcaa ttacaggctt tttcaatatg
     4801 cgcaacgtgt gttcgtcgct gttatattct cctgcggaga aataaaaatg aacatctttt
     4861 tgcaagttgg tatatctgta cttgaactta tcgggagccg ttttttccat tttataattc
     4921 ttgccgccgt cgttgatata cacttcattg ggcaaggttt ctcctttgat acttaaggcc
     4981 acttcatagt cctgatattg tatggctttg agttttttat tttccaaaac aaaatcaaag
     5041 ggtgctttct tggcaaatat tacatcggga ttagccagcc gtttattgct ttctttaaaa
     5101 atctgcggag ctatcaaaag tatcattgcg atagccaata agggcggcaa cacgtacttg
     5161 aggtattttt tgttttctga tagattgaca gcttgtgtaa agggtaccaa cttaatgcca
     5221 tccatttttt ggttaatgga cgcttctatc aaatcgcggt tgtaggcatt ttgtgattgc
     5281 gttttgagtt gcaatatgtt cagcagtttg tcttttaccg gttggaaatg attgccgata
     5341 attgttgcag ccgtttcatg tgtaatgaca ttgcctaaat tcagataatg aaataaaggc
     5401 aacagcaccc aataagccag tgcaaaaatg aaaacagcca taaaggagaa caaaataaat
     5461 ttgcgtacag acgaagaaaa ataaagcttg tactcaaata cgctggcaaa caaataatag
     5521 accgttacca gagccaaaaa taacaatata ccccgcaaaa gtttattcaa ataatacttt
     5581 cgaataaacc cgtcaagccg ttctataagt agctgataat tgttttccat ggcacaattg
     5641 caaactgtgt aaatatatac agcttggatg agatttttag cgttattttg ggttaaaatt
     5701 agtagtaaag ccgtatctgt caaaaatgat actgactgat aaacttatga aacgcatttt
     5761 tatagcgtgt atcctgcttg tttgtcttca atgtataata ctgtttaagg atgtccggca
     5821 gaggcaattc atccggttgc cagactacgc ccattttgcg ttcttgcatt aaatgtgcca
     5881 aatactcctg ttcggtttgt ccatgcgttg gaatataaat ggctttcttg ttcaagacag
     5941 ataagtccat gatggtagaa tatcccgacc tgcagatgat ggcagcagcc ttatgataag
     6001 ccgctaataa atcatttgtt tggagcagtg ggatgagggt aacaaactct tttactttca
     6061 ttccttcatc atccggcttt cctctcacca aaagagtttt cttcttttgc tgattacaga
     6121 tttttatcag cttggcttcc atctcactgc gctgtggttc tgggccgctt agtacgatca
     6181 agatttcaaa atctatatcg tcgatatacg atgaatgttt aattttatta gtaaacctgc
     6241 tcatcacccc gatataatga tgcggtatag gaaaggtaat gccgtgtgct aatttgccgg
     6301 ataaattgtg cactcctttt atatccggaa tccaaagctc atggaatggc ttgatgaaac
     6361 gcaagtg
//
LOCUS       DAOQII010000140         6277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7003_length_6277_cov_5.583574, whole genome shotgun sequence.
ACCESSION   DAOQII010000140 DAOQII010000000
VERSION     DAOQII010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7003_length_6277_cov_5.583574"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /gene="groEL"
                     /locus_tag="PLF48_07605"
     CDS             <1..1001
                     /gene="groEL"
                     /locus_tag="PLF48_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNF49225.1"
                     /translation="MDFLEIIDLKDKKVSSMKDLLPVLEKVVQTGRALLIIAEDVESE
                     ALATLVVNRLRGSLKIAAVKAPGFGDRRKAMLQDIAILTAGEVVTEEMGMKLEDTQLN
                     QLGVAEKVVIDKDNTTIINGSGKKADIQARVNQIKAQIETTTSDYDREKLQERLAKLS
                     GGVAVLYVGAATEVEMKEKKDRVDDALHATRAAVEEGIVPGGGVALIRAQSALDKLKG
                     ANEDEQTGVEIVRIALESPLRQISENAGVEGSIVVFNVKNGKKDYGYNAKTETYENLY
                     KSGVIDPTKVTRVALENAASIASMILTTECAIVEKPSKEAPAMPGGGMPGGMGGMM"
     gene            1444..1830
                     /locus_tag="PLF48_07610"
     CDS             1444..1830
                     /locus_tag="PLF48_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49226.1"
                     /translation="MLWQAYCDGYANHCGKQFADWSAAYRRFSQNRISNATLFQTVIN
                     KVVEKQSNSDYVVADMNDTLIRKRGKKIPGTGWKRDPLGSAFHANFVWGQGFLQISLC
                     LSKLDKIWNYNSDKCFYFFINQQQQA"
     gene            complement(1956..2504)
                     /locus_tag="PLF48_07615"
     CDS             complement(1956..2504)
                     /locus_tag="PLF48_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49227.1"
                     /translation="MKKLLQIFMALLVITAIACTKEGPQGPAGTNGTNGTDGNANVKV
                     LLWNTPPTGVANGINRYDFTFTHANITTDIINNGAILVYVSYSSTSWDYAPYWYSTTN
                     YASVFFYNSPTPKVVLSTAASFVDKVRIVLIGGPSSGTTVVNRMSSAWNKLTEIEKAK
                     LLYPDVDFNNYSTVNTYFNFKD"
     gene            2671..3354
                     /locus_tag="PLF48_07620"
     CDS             2671..3354
                     /locus_tag="PLF48_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49228.1"
                     /translation="MKKSNLLIGIMCITSIFTACKKENNTEVKVLFHSSGKTAVGRSF
                     TFNKFNVYLSDFYLLNAIGDTTYIKDVMLAKLNEDNSFSTEVPKEIQKFGFSFGLSPQ
                     LNNSVPESYPAAHPLSVEQDMYWGMLKYRFLVIDGGNVDTSYARNQTPVMPFSYHLGT
                     DTLYRKLVFNRPVVDGDKVKIVLDIDKLFVLDSTRFNLNNFSNHSDPGQIPNAIKITD
                     AFISGISVQ"
     gene            3356..4444
                     /locus_tag="PLF48_07625"
     CDS             3356..4444
                     /locus_tag="PLF48_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF49229.1"
                     /translation="MIKPKNIFGIFGAITVVCIYIIACTKDTPSEAPYVTTPYSPPVI
                     SGFRPMPQDSSNPMTLEGIALGEKLFFDPILSKNNTISCSSCHKKENAFSDPRKFSLG
                     VNDSVGKRNALALINLAWYPNNFFWDGRAHSLNEQAVFPVEDTKEMNLSWTEAISRLQ
                     NNATYTDMFKKAFNTKTITRDWVTKALTQYEKTLLSYNAPIDKYFRGEAPLDTFVLRG
                     MQVFNSERGDCFHCHTTKELFVHPTRFFVNNGMDLAPTGTEFADLGLGAVTHNPADNG
                     KFKLPTLRNLAFTAPYMHDGRFNTLDEVIESYNRGPQNSPTVEPILIEKANIRLTNTG
                     VWGLQLSPHEKFCLKQFLLALSDSSFVN"
     gene            4579..4959
                     /gene="gcvH"
                     /locus_tag="PLF48_07630"
     CDS             4579..4959
                     /gene="gcvH"
                     /locus_tag="PLF48_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNF49230.1"
                     /translation="MNFPTELKYSKEHEWLRIDGEIGTIGITEFAQKELGDIIYVDID
                     CVGDSIDKDAIFGTVEAVKTVSDLFMPVAGEVLEFNEALKDKPELVNSKPYEDGWMIK
                     IRITDAGQVSDLLDSDAYQALIGK"
     gene            4973..5359
                     /locus_tag="PLF48_07635"
     CDS             4973..5359
                     /locus_tag="PLF48_07635"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNF49231.1"
                     /translation="MTLSRFLYKVIPALLWSGMIFFLCFLPGSKIPKEDWLDKIYFDK
                     IVHAGLYFILFFLLLRIPFQINIKTILLAIIFCMVQGIIIELVQGSSWIEHRSFDAWD
                     IVANFFGLMFACTLYYFKLLPVHRNK"
     gene            5392..6207
                     /locus_tag="PLF48_07640"
     CDS             5392..6207
                     /locus_tag="PLF48_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49232.1"
                     /translation="MQFGILDYWEILKQYTNPFDIIKGTILYILIFFFVTLVLVFVFR
                     KRILVKRQNAMLKYLSYAYFIVLPLLSAFFGFKYGMANAVEKDLIKHTDSYSTAIENS
                     FLADSSGILKAVLSGDVNMAKNTPGISVNNSIDVLTYVIYDSYGATLEKAAKANNTVT
                     SKVAGLFLKVTQSMVISKMVKKTLHNVISEKVGLGEELSDKLMETKLNELIKNGIFKE
                     IAIIQIKQIFGGIKNGVLIMLSLVFGACIIEIGIAHYYLNKQTAHPSTSELNS"
BASE COUNT         2031 a         1039 c         1213 g         1994 t
ORIGIN      
        1 ttatggattt tttagaaatt attgatttaa aagataaaaa agtatcttct atgaaagact
       61 tgttgccagt tttggaaaaa gttgttcaaa ctggaagagc tttgttgatc attgctgaag
      121 atgtagaaag cgaagcattg gcaacattag tagtaaaccg tttacgaggt tcattaaaaa
      181 ttgcagcggt taaagctccg ggctttggag acagacgtaa ggcaatgctg caagatatcg
      241 ctatattgac agctggagag gtggttacag aagaaatggg tatgaaactg gaagacacgc
      301 aattaaacca gttgggagtt gctgaaaaag tagtaattga taaagataat acgactatca
      361 tcaatggatc tggtaaaaaa gcagatattc aggctcgtgt aaaccagata aaagctcaga
      421 tagaaaccac aacttctgat tatgacagag aaaaactgca agagcgtttg gctaaattat
      481 caggcggtgt ggccgtatta tatgttggtg cagctacaga agtggaaatg aaagagaaaa
      541 aagaccgtgt agatgatgca ttacatgcta ctcgtgccgc tgtagaagaa ggcattgtgc
      601 cgggaggtgg tgttgcatta atccgtgctc aatctgcttt ggataaattg aaaggagcta
      661 atgaagacga gcaaacgggt gttgaaatcg tgagaatagc attggaatct ccattgcgtc
      721 agatttctga gaatgcaggc gtagaaggct ctattgttgt atttaatgtg aaaaacggca
      781 agaaagacta tggttataat gccaaaacgg aaacgtatga aaatttatat aaatccggcg
      841 taattgatcc aactaaagta actcgtgtag ctttagaaaa tgcggcatcc attgcttcca
      901 tgattttgac tacggaatgt gcaatagtag aaaaaccgag caaagaagca cctgcaatgc
      961 cgggtggtgg catgccgggc ggtatgggtg gtatgatgta atcaacccgc ataatttaaa
     1021 tagtaaaagc gtcggcaatt ttgccggcgt ttttattttg gtgctttaca actaagtaaa
     1081 tataattttt tttaattaag cttatatata tacatatgtt ataataatgt attctaatgt
     1141 attgatgtga ttaagttaat caaatgagct tccagtgagc agtggtatgt gaactttcca
     1201 gctgccgtgg cacaagctat tgagtttaac aaaggtgaag aggttgagtg gttgatttat
     1261 gaccaccaga atttagtctt gcacaggagt gacaaaacag tagcatcctt gaaaaaaaac
     1321 tgaatcaaaa aaacgaaaac tttacactat tgcactcatt cacccgttta ctgaattctt
     1381 ctaccgccca cttgaaaaac gattagccaa tcgggtactt gagttggctg tcagcatact
     1441 gtcatgttat ggcaggcata ctgtgacgga tatgctaacc attgtggtaa acaatttgca
     1501 gactggagcg cagcgtacag acgctttagc caaaacagga tatccaacgc cactttattc
     1561 caaacggtca ttaacaaggt agtggaaaaa caaagtaact ctgactatgt agttgctgat
     1621 atgaatgaca cattaatcag aaagagaggt aaaaaaatac ccggtacggg ttggaaacgc
     1681 gaccctcttg gttcagcatt tcatgccaac tttgtgtggg gacagggatt cctgcaaatt
     1741 tccttatgct tatctaagtt agacaaaatt tggaactaca acagtgataa atgtttttat
     1801 ttcttcatca accaacaaca acaggcatga agcttgcaaa acgtacaaaa tcccatacat
     1861 tccactatgg tttaggtagg taaatagcca aagtataaaa aaagtgcgac cccgaaggcg
     1921 ccgcactttt tttttattaa taaattgttt tttaactaat ctttaaaatt aaaatatgta
     1981 tttaccgtac tataattgtt gaaatcaaca tcaggataaa gtaattttgc tttttcaatt
     2041 tctgttaatt tattccatgc tgatgacatt ctgttgacaa cagttgttcc acttgaaggt
     2101 ccaccaatta atacaattct tactttatct acaaaacttg cagctgtact taggacaact
     2161 ttaggagttg gagaattata aaaaaataca cttgcataat tagttgtaga ataccaatat
     2221 ggcgcatagt cccatgatgt tgaactgtaa gatacgtaaa caagtatagc cccattgttt
     2281 attatatctg tagttatgtt cgcatgtgta aaagtaaaat cataacgatt gataccattg
     2341 gcaactccag ttggaggcgt attccataag agtactttta catttgcatt accatctgtt
     2401 ccgtttgttc catttgtacc ggcaggtcct tgtggtccct ctttggtaca tgctatggct
     2461 gttatcacta ataacgccat aaaaatttga agtagttttt tcataataaa taatttttaa
     2521 tacttcaaaa ctatttcata tattctgaat aggtataatt aatgagtatt cggtacgttt
     2581 aattgagtat tcggtaatag tgcaaacttt tgaactgtta attcctctca aatttatctt
     2641 gctgctgttt ttatttcata actttaacac atgaaaaagt ctaatttgct gattggcatc
     2701 atgtgtatta catccatttt taccgcatgt aaaaaagaaa ataatacgga agtaaaagtt
     2761 ttatttcatt catcaggaaa aacggctgta ggcagaagtt ttacttttaa taaattcaat
     2821 gtttatcttt ccgattttta tttgctcaat gccataggcg atactactta tatcaaggat
     2881 gtaatgcttg cgaagttaaa tgaagataat tcattttcta cggaagttcc taaagaaatt
     2941 caaaaattcg gtttttcctt tggtttaagt ccgcaactta acaacagcgt acccgaatct
     3001 tatcccgctg cacatccgct atctgttgag caagatatgt actgggggat gctaaaatat
     3061 cgttttctgg ttattgacgg cggaaatgta gatacctcat acgctcggaa tcaaactcca
     3121 gtaatgccat tttcctatca tttaggtacg gatacgttat atcgaaaatt ggtatttaac
     3181 cgacctgttg ttgatggtga taaagtaaaa atagtgctgg atatcgataa actttttgtg
     3241 ttagacagca ctcgatttaa cttgaataat ttcagcaatc acagcgatcc gggacaaatt
     3301 cctaatgcaa ttaaaattac tgacgcattt atttctggta taagtgtgca ataatatgat
     3361 taagcctaaa aatatatttg gaatttttgg tgcaattact gtagtgtgca tatatattat
     3421 tgcttgtaca aaagatactc cttccgaagc tccttatgta actacaccct attcgccacc
     3481 ggtaatttca ggcttcagac ctatgccaca ggattcgtcc aacccaatga ccttggaagg
     3541 cattgcactg ggagaaaaac ttttttttga tcccatttta tctaaaaaca atactatttc
     3601 ttgctcaagt tgtcataaaa aagaaaatgc attttccgac cctcgcaaat ttagtcttgg
     3661 tgtaaatgat tcggtgggta agcgtaatgc tttagcttta atcaatctgg catggtatcc
     3721 caataatttc ttttgggatg gtcgggctca ttctctaaat gagcaagctg tttttcctgt
     3781 agaagatacc aaagaaatga atttgtcttg gacggaagcc atatctcgcc tacaaaataa
     3841 tgctacttat acggatatgt ttaaaaaggc atttaacacg aagaccatta cccgcgactg
     3901 ggtaacaaaa gccttaacgc aatacgaaaa aacattactc tcgtataatg caccaattga
     3961 taaatatttc agaggagagg ctccattaga tacttttgtt ctgaggggta tgcaggtatt
     4021 caattccgaa cgcggcgatt gctttcattg ccatacgacc aaagaactgt ttgttcaccc
     4081 aactcgcttt tttgtaaata atggaatgga tcttgcacct acaggcactg aatttgccga
     4141 cttaggactt ggtgccgtta cacacaatcc ggcggataat ggaaaattta agttgcccac
     4201 actgcgcaat ctggcattta cagcacccta tatgcatgac ggaagattca atactttaga
     4261 tgaagtgata gaatcataca atcgtggacc acaaaactca ccaacggtag agcctatttt
     4321 aatagaaaaa gcgaatatcc gcttgaccaa taccggagtt tggggcttgc agctttcacc
     4381 acatgagaaa ttttgcctga agcaattttt actggcttta tctgattctt ctttcgtgaa
     4441 ttaaaattac gattgcactt cgattgatta attaatgctg cctatttttt gtgtagatgt
     4501 catagttttc ttgtgccggt ttttataaac agcatatttc aataaacaaa aaaccttatc
     4561 ttagcaaaaa agaattttat gaattttcca acggaattaa agtactctaa agaacacgaa
     4621 tggttgcgta tagatggtga aatcggtacg attggcatta ctgaattcgc tcaaaaagaa
     4681 ttgggtgaca ttatttatgt agatattgac tgtgtaggcg attctataga taaagatgcc
     4741 attttcggaa cagttgaagc cgttaaaacg gtttctgatt tatttatgcc ggtggcaggt
     4801 gaagtacttg aatttaatga agcattaaaa gataaacccg aactcgtaaa cagtaaacct
     4861 tatgaagacg gatggatgat taaaattaga atcactgatg ccggtcaagt atcggacttg
     4921 ctggatagtg atgcttatca agctttgata ggcaagtaat gaagtttgta cattgacact
     4981 ttcaagattt ttatacaaag tcatacctgc gcttttgtgg tcgggcatga ttttcttttt
     5041 gtgctttctg ccgggtagta aaataccaaa agaagactgg ctggataaga tttattttga
     5101 taaaattgta catgcgggct tatactttat cttgtttttt ctgctcttaa ggattccatt
     5161 tcaaatcaat attaaaacca ttttgctggc tataattttt tgtatggttc aaggcattat
     5221 aatagaatta gtacaaggaa gctcgtggat cgaacaccga agttttgatg cttgggatat
     5281 tgtggctaat tttttcggtt tgatgttcgc ttgtacacta tactatttta aacttctgcc
     5341 tgtacataga aataaataaa ataatagtat tatatttcaa aaaaaattag aatgcagttc
     5401 ggtattttag actattggga aattctcaaa caatacacaa atccgttcga tattattaag
     5461 gggacaatat tgtacatatt aatcttcttt tttgtaacat tagttttggt atttgtattc
     5521 aggaagcgca tattggttaa aagacaaaat gcgatgctaa aatatctttc atatgcctat
     5581 tttattgtac ttcctctttt atccgctttt ttcggattta aatatggtat ggcaaatgca
     5641 gttgaaaaag acttgataaa acatactgac tcgtattcaa ctgccattga aaattcattt
     5701 cttgcagatt cttccggaat tttgaaggca gtactatcgg gtgacgtgaa tatggcaaaa
     5761 aatacgccgg gaataagtgt caataactca attgatgtgc tgacctatgt tatttatgac
     5821 agctatggag caacattgga aaaagcagca aaggcaaata atactgttac atccaaagtt
     5881 gcaggtttgt ttcttaaagt cacacaatca atggtcattt ctaaaatggt aaaaaaaaca
     5941 ttgcataatg tgataagtga aaaagtcggt ttaggagaag aactttccga taaattaatg
     6001 gaaactaaat taaacgagtt aatcaaaaac ggcatcttta aagaaatcgc cattattcag
     6061 attaagcaaa tttttggagg cataaaaaac ggagtgttga ttatgctgag tcttgttttt
     6121 ggggcgtgta taattgaaat tggtatagct cattactatt tgaataagca gactgcacat
     6181 ccatcaacat cagagcttaa ttcataacac aatttcacag cttttttgct gttttttgca
     6241 taaattttga tacgtttatt ccgtgaaaaa atactgc
//
LOCUS       DAOQII010000141         6209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7131_length_6209_cov_3.817680, whole genome shotgun sequence.
ACCESSION   DAOQII010000141 DAOQII010000000
VERSION     DAOQII010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7131_length_6209_cov_3.817680"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1410
                     /gene="rocD"
                     /locus_tag="PLF48_07645"
     CDS             151..1410
                     /gene="rocD"
                     /locus_tag="PLF48_07645"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNF49233.1"
                     /translation="MKNLSSQEIIALEDKYGAHNYHPLPVVLAKGKGIHVWDVEGKSY
                     LDFLSSYSAVNQGHCHPRIIKVMQEQAETLTLTSRAFHNNILGVYAEYITSLLGFDKV
                     LPMNTGVEGDETAFKLARRWGYDVKGIEKNKAKVVVCTNNFMGRTIAAISCSNDPSSY
                     GGFGPYVPNIDHVPYNDTVALEEKLQDKEVCAFFVEPIQGEAGVVVPDEGYLKKVRAL
                     CTKYNVLFVADEVQTGIARTGKMMACDYEEVKPDILILGKALSGGTMPVSAVLANDDI
                     MLVIKPGEHGSTFGGNPLACRVAIESLKVTVEEKLAENAFAMGEIFRNELRNFKHPIV
                     KTVRGKGLLNAVEIITDDNQEHTWNNPGELAWNICLKMRDNGLLAKPTHGNIIRFAPP
                     LIINEAQILEATDIIKTSIEQTVGVTA"
     gene            1470..2312
                     /locus_tag="PLF48_07650"
     CDS             1470..2312
                     /locus_tag="PLF48_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF49234.1"
                     /translation="MKLYTIDTGYFKLDGGAMFGVVPKTMWQKLNPPDENNMCTWAMR
                     CLLLQFDNRLMLIDTGMGDFIDATYRKRYQPIGDDGLESSLRRYNFSPEDITDVFFTH
                     LHFDHCMGALKDDGNGNPTLRFPNATHWTHSIQWQSAMHPNEREKASYLIPYLQAIEN
                     SGKLHFADMEYIGTESIKTEVVNGHTESMMLCHIYLENKIITYCADLIPSHHHIKLPY
                     IMAYDIRPLETLKEKTALLNNAFSENRILFFEHDKDVACCTLKNTEKGIEFDKTGTLE
                     ELLQ"
     gene            2309..3814
                     /locus_tag="PLF48_07655"
     CDS             2309..3814
                     /locus_tag="PLF48_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49235.1"
                     /translation="MKIKLILVYVFLLLSFRAWSQIEKGDKLYQDYKYAQAIEYYKPE
                     AEKGNIRAIRKIAECYKKINDYENAEKYYALVVADKNAIVKSFFYYGQALMNTGKYDT
                     AYIWLEKYLKSKPGNDSFLVKNLMESCLNAGKTENTNRKVKVWNFESLNTAAGEFGIY
                     PVQEGFVFTSTRQGKLNGTSGSGFQQVFFAKPKGNAYTVESIKGVMNTKNYNSGPACV
                     DTLKGIIYYTKNNFQYGDAVTNKKGDVTLKIFSAEFRNDNVKNQHEMEFNEAESSCAF
                     PSINKQGNVIYFTSDRGGGYGGKDIYYSIYQNGKWSKSKNAGKNVNTSGDERYPFIHP
                     DGTLYFSSNGLTGYGGMDIYKCTPNKIGEFGKPENMGTQFNSPTDDFAFYLDDDYNKG
                     YFASDRAGGKGMDDIYAFQYLNIPFELNFYSNTQPLDSVQVCIEKDGKTLETVLSTTK
                     INKELDPDAVYVFEAYKDGYLLNELSIKTSQKKQAISKSLSLTLSKPEAEE"
     gene            3848..4285
                     /locus_tag="PLF48_07660"
     CDS             3848..4285
                     /locus_tag="PLF48_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49236.1"
                     /translation="MENLIFRDAVFSDCESLAFLMAQLGYPSTNEQMQDRYTIIKKQE
                     DYKTIIAEMDGKIIGMAGMIKLYFWEQDGFYLKIQAFVIDKAYHRKGIGKLLMQECEK
                     YARLKKAKVIALTSGNRSEREDAHHFYPAMGFTASSTGYKKNL"
     gene            complement(4303..4950)
                     /locus_tag="PLF48_07665"
     CDS             complement(4303..4950)
                     /locus_tag="PLF48_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNF49237.1"
                     /translation="MRFSPKINHDSVLLEQLIRLGEGVSLDFKQTISDKKKIARTICA
                     FANNKGGKLLVGVRDNGDIIGCEPEEEMYMLFEAAEHFCDPPVDIYFTIYELEDDISV
                     IEAEIKNSLKKPHFAIDENDEWQIYMRSNDKTMMASSLSAKLIDRDEDAEAITDSKQQ
                     FVIDYLTTHESITAKQLTLKLNLSLQRANRILISLLQGGHILHHKDNRGDYYSLR"
     gene            5058..5501
                     /locus_tag="PLF48_07670"
     CDS             5058..5501
                     /locus_tag="PLF48_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49238.1"
                     /translation="MSYLEALYGSQYAELVQNGHSGENGRTNGNLFLTAIIIMSFLCG
                     FGVLMLILSALHFPANEWLNNIAVSTGLTGKKMGIFVALPLLIIIYFILNKSLGNEEQ
                     YKKYCAAYMQATNEEKESANKKTLRPLFGLIILFFVIIFLSTIIH"
     gene            complement(5541..>6209)
                     /locus_tag="PLF48_07675"
     CDS             complement(5541..>6209)
                     /locus_tag="PLF48_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49239.1"
                     /translation="FIFMKCMKAVNANKKQNCRMKMNRHHKLFFLLISWSLLPLSCKY
                     FGLNDPYKIEMPENRISPEQAHEEKYENTINESHKFQPLTETKTEDVRFILYNAIKYN
                     IIDPETNVGEGFDYYIMDIGIDNFSRHAFNIRAFTNSCKLTNDTPDFAYQNIPNILKM
                     YALQRDSTELDFQAVRNFTYDTIPGRTYCRAKLFAYEVSAEDKSPLYFHYKLNGKEYK
                     YEIR"
BASE COUNT         2057 a         1112 c         1176 g         1864 t
ORIGIN      
        1 caatactctt tacttaatac ttaatactca atacttacta cttactactt actactcccc
       61 actttatgct ccccactaat tccttttatt caccattagc cccttttcca attatcacga
      121 aaaatcgtta tttttgccaa aattgtcggc atgaaaaatt taagttctca agaaatcatc
      181 gcactggaag acaaatatgg tgcgcacaac tatcacccgc ttccggtagt attagccaaa
      241 ggcaagggta ttcacgtttg ggatgtggaa ggaaaatcat atttagactt tttaagctcg
      301 tattctgctg ttaatcaagg acattgtcac cccagaatca ttaaagtaat gcaagaacaa
      361 gcagaaacac ttacccttac atcaagagct tttcacaata atattttagg cgtgtatgca
      421 gaatacatca cttcgctatt gggattcgac aaagtattgc ccatgaatac cggtgtggaa
      481 ggcgatgaaa ctgcatttaa attagcccga cgctggggat atgatgtgaa aggcattgaa
      541 aaaaacaaag ctaaagtcgt agtatgtacg aacaatttca tgggcagaac gattgcggcc
      601 atatcatgtt ccaatgaccc ttcttcttac ggcggatttg gtccttacgt acccaatatc
      661 gatcatgtgc cttataatga taccgttgct ctggaagaaa aactgcaaga caaagaggta
      721 tgtgcctttt ttgtagaacc tatacaaggc gaagccggag tcgtagtgcc tgatgaaggc
      781 tatctcaaaa aagtaagagc actttgcaca aaatacaatg tgctttttgt tgccgatgaa
      841 gttcaaaccg gaattgcccg taccgggaaa atgatggctt gtgattatga agaggtaaaa
      901 ccggacattt tgattttagg aaaagcacta agtggtggca ccatgcctgt aagtgctgtt
      961 ttggcaaatg atgatatcat gctggtgata aaaccgggtg aacatggctc tacttttggt
     1021 ggcaatcctc tggcttgtcg ggtagcgatt gaaagtttga aagtaactgt agaagaaaaa
     1081 ctggcagaaa atgcttttgc catgggtgaa attttcagaa atgaattgcg aaacttcaaa
     1141 caccctatcg taaaaacagt aagaggcaaa ggcttgctga atgccgttga aatcattacg
     1201 gatgacaacc aagagcatac ttggaataat ccgggagaac tggcttggaa tatttgcttg
     1261 aaaatgcgtg ataatggcct tttggcaaaa ccaacgcatg gcaatattat tcgttttgct
     1321 ccacctttga ttatcaatga agctcaaatt ttggaagcga cagacatcat caaaacttct
     1381 atagaacaaa cagtaggcgt aacggcgtga gatgatgaat cattttttga tttgtaagtt
     1441 tcgtttacgc atctttatat ttttgcccaa tgaaattata tactattgat acgggttatt
     1501 ttaaattaga tggtggtgca atgtttggtg tagtgcctaa aaccatgtgg caaaaactca
     1561 acccgcctga tgaaaacaat atgtgtacct gggcaatgcg atgtctgtta cttcaatttg
     1621 acaacagact gatgcttata gatacgggta tgggcgattt tattgatgct acctacagaa
     1681 aacgctatca acccatcggt gatgacggtt tagaaagttc tttaagacga tataactttt
     1741 ctcccgaaga tatcacagat gtgtttttca ctcatttgca tttcgaccat tgcatgggtg
     1801 cattgaaaga tgacggcaat ggaaatccca cattgcgttt tccgaatgcc acacactgga
     1861 cgcacagcat acaatggcaa tctgccatgc atcctaacga aagagaaaaa gcatcatatt
     1921 tgatacctta tttgcaagct attgaaaatt cgggaaaact gcattttgcc gatatggagt
     1981 atatcggtac ggagtcgata aagactgaag tagtaaacgg acatacggag tcgatgatgc
     2041 tgtgtcatat ttatctcgaa aataaaataa ttacctattg tgccgatttg attccgtcac
     2101 atcatcacat aaaactgcca tatattatgg cttatgatat acgccctctg gaaacattaa
     2161 aagaaaaaac agccttatta aacaacgcat ttagcgaaaa tcgcatctta ttcttcgaac
     2221 atgataaaga tgtagcctgt tgtaccctga aaaatactga aaaaggtata gaatttgata
     2281 agacaggtac attagaagag ttgttgcaat gaaaataaaa ttaatcttag tttacgtttt
     2341 cctcctcctt tcatttcgtg catggtcaca aattgaaaaa ggcgacaaac tctatcagga
     2401 ttacaaatat gcccaggcca tagaatatta taaaccggaa gcagaaaaag gcaatatcag
     2461 agctatacgc aaaatagcag aatgctacaa gaaaatcaat gattatgaaa atgccgaaaa
     2521 atattatgcc ttagtggtcg cagataaaaa cgctattgta aagtcatttt tttattacgg
     2581 gcaagcccta atgaatacgg gcaaatatga tactgcctat atatggctcg agaaatatct
     2641 gaaatccaag cccggaaatg acagttttct ggtaaaaaac ttaatggaat cttgcttgaa
     2701 tgccggaaag acagaaaata ctaacaggaa agtaaaagtt tggaattttg aatcactgaa
     2761 tactgcagcc ggcgagttcg gaatttatcc tgtgcaggaa ggatttgtct tcacctctac
     2821 ccggcaagga aaactgaatg gcacatccgg cagtggcttt cagcaagtat ttttcgccaa
     2881 accaaaagga aatgcatata cagtagaatc cattaaggga gtgatgaata caaaaaacta
     2941 caacagcggt cctgcttgcg tggatacact taaaggtatt atctattata ctaaaaataa
     3001 ttttcaatac ggcgatgccg taaccaataa aaaaggtgat gttacattaa aaatattttc
     3061 agctgaattc agaaatgata atgtgaaaaa tcaacatgaa atggaattta atgaagctga
     3121 atcttcttgt gcatttccct ctatcaataa acaaggcaat gtcatatatt tcacatctga
     3181 tcgcggtggc ggatatggag gaaaagatat ttattattcc atttatcaaa atggaaaatg
     3241 gagtaagtcc aaaaatgcag gaaaaaatgt gaatacatcc ggtgatgaac gctatccttt
     3301 tatccatccc gatggtacgc tttatttctc gtcaaatggt ttaactggct acggaggtat
     3361 ggatatttat aaatgtacac caaacaaaat tggtgagttt ggcaagcctg aaaatatggg
     3421 cactcagttc aactctccta ccgacgattt tgccttttat ctggatgatg attataataa
     3481 gggctatttt gcttccgatc gtgcaggagg caaaggcatg gatgatattt atgcatttca
     3541 atatcttaac ataccttttg agctaaattt ttacagcaac acacaaccat tagacagtgt
     3601 gcaagtatgc atagaaaaag acgggaaaac tttggaaacc gtgttgagta ccacaaaaat
     3661 aaataaagaa ttagaccccg atgctgttta tgtttttgaa gcttataaag acggttattt
     3721 gctaaatgaa ttaagcataa aaacttcaca aaaaaaacaa gccattagca aaagtcttag
     3781 ccttacacta agcaaacctg aagcagaaga ataaatataa accaactgta tacaataatt
     3841 ttatactatg gaaaatttaa ttttcagaga tgctgttttt agtgattgcg aatcactcgc
     3901 ctttctgatg gctcaattgg gatatcctag cactaacgaa caaatgcaag acagatatac
     3961 tataataaaa aaacaggaag attataaaac tataattgca gagatggatg gaaaaatcat
     4021 cgggatggcc ggcatgatta aactatattt ttgggagcaa gatggttttt atttaaaaat
     4081 tcaggcattt gtcattgata aagcttatca cagaaaaggc ataggtaaat tactcatgca
     4141 agaatgcgaa aaatatgccc gacttaaaaa agcaaaagta attgcattaa caagtggaaa
     4201 tcgttcagaa agagaagatg ctcatcattt ttatccggca atgggtttta cagccagctc
     4261 aacaggctat aaaaagaatc tataaataag tcaatttact tcttatctca agctgtaata
     4321 atcacctctg ttatctttat gatgcaggat atgcccccct tgcaaaagtg aaatcaaaat
     4381 tcggtttgcc cgctgtaacg aaagattgag cttaagggta agttgtttag ctgttataga
     4441 ttcatgtgtt gtaagatagt cgatgacaaa ttgttgcttg ctgtctgtaa tagcttcagc
     4501 atcctcatct ctgtcaatca acttagctga caaagatgac gccatcatcg ttttgtcatt
     4561 actgcgcata taaatttgcc attcatcgtt ttcatctatg gcaaaatgtg gttttttcag
     4621 actgtttttt atttcagcct ctataacaga aatatcatct tccagctcat aaatggtaaa
     4681 atatatatca acaggcggat cacaaaaatg ctcggcagct tcaaagagca tgtacatttc
     4741 ctcttcgggt tcgcatccga taatatctcc attatcacgc acacctacca gtagtttacc
     4801 gcctttgttg ttggcaaaag cacagatagt tctggctatt ttttttttgt ctgaaatcgt
     4861 ttgcttaaaa tccaggctca ccccctcacc tagcctgatc agttgttcta atagtacact
     4921 atcatggttt atttttggtg aaaatcgcat tagtaaatgt agcaaatttt gaaatacacc
     4981 atacccgcat ttttaaagga atgtcattta aatgtaaaag gtttttgcat attagtttgt
     5041 aattttgtaa aaaaaagatg agttatctgg aagcgcttta tggcagccaa tatgctgaac
     5101 tggttcaaaa cgggcatagc ggtgaaaatg gcagaacgaa tggaaatcta tttttgactg
     5161 caataatcat catgagtttt ttatgtggat ttggtgtgct tatgctaata ctgagtgcgt
     5221 tgcattttcc ggcaaatgaa tggctgaata atatagcagt ttctaccgga ctaaccggaa
     5281 aaaaaatggg catatttgta gccctgccgc tgttgataat tatctatttt atcctcaata
     5341 aaagtttagg aaatgaggag caatacaaaa aatattgtgc cgcttatatg caagcaacta
     5401 atgaagaaaa agaaagtgct aataagaaaa cacttcgccc gctattcggc ttaattattt
     5461 tgtttttcgt gattattttt cttagtacaa tcattcactg aaaacgctgc caatacaagt
     5521 gcatttaaca agaaactaat ttatcttatt tcgtacttat attccttgcc attcagttta
     5581 taatgaaaat acaacggact tttgtcttct gcgcttactt cgtaagcgaa taattttgcg
     5641 cgacaatagg tgcgacctgg aatggtgtca taagtaaagt tgcgcactgc ctgaaaatcc
     5701 agctcggtag aatcccgttg cagtgcatac atttttagaa tattagggat attctgataa
     5761 gcaaaatcag gggtatcatt agtcagcttg caggagttgg taaatgccct gatattaaat
     5821 gcatgccggc tgaaattatc tataccgatg tccataatat agtaatcaaa tccttcacct
     5881 acattcgttt caggatctat gatgttgtac ttaatagcat tatataatat aaaacgcaca
     5941 tcttctgttt tcgtttccgt caaaggttga aacttatgac tttcattaat ggtattttca
     6001 tatttttctt catgagcttg ctcgggagaa atacggtttt caggcatttc aattttatac
     6061 ggatcattca acccaaaata cttacaagaa agcggtaata aactccagct aataagcaga
     6121 aagaataact tatgatgtct gttcattttc attcggcaat tctgtttttt gtttgcgttt
     6181 actgccttca tacatttcat aaatataaa
//
LOCUS       DAOQII010000142         6205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7136_length_6205_cov_4.953496, whole genome shotgun sequence.
ACCESSION   DAOQII010000142 DAOQII010000000
VERSION     DAOQII010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7136_length_6205_cov_4.953496"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..652
                     /locus_tag="PLF48_07680"
     CDS             29..652
                     /locus_tag="PLF48_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF49240.1"
                     /translation="MEDLLQIEAFKSSPALVEKLQAYSIRKEYKAGSIILNENVHIRS
                     IPIVTKGTLKVIRTEDDGREILLYYIKAGESCIMSFLGGMHNETSKVRAEVEDHAEIL
                     FLPIDKVSLFIKEYPQWLDYIFRLYHKRFEELLEIINAIAFKKVDERLLDLLQKKKEL
                     TGSNTLIITHEQLANELGTARVVVSRLLKQLEEIGNVKLGRNKVTLR"
     gene            849..2258
                     /locus_tag="PLF48_07685"
     CDS             849..2258
                     /locus_tag="PLF48_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF49241.1"
                     /translation="MKIEQIYTGCLAQGAYYITSNGEAAIIDPLRETHPYLDRLSKDG
                     VKLKYIFETHFHADFVSGHLDLSKKTNAAIVYGPSAQPEFEAIIAEDNQAFEIGNIKI
                     KVLHTPGHTMESTCFLLIDENGTDSALFSGDTLFLGDVGRPDLAQKAAHLTQDELAGL
                     LYESIYNKILPLADDITVYPAHGAGSACGKNMMKQTVDTLGNQKIMNYALNQPNKEAF
                     VKAVTDGLLPPPAYFGMNVAMNKKGYERFDDVLQHSLKALSADEFEVVAETSNALILD
                     TRSDIDFAVGYIPQSINIGLNGDFAPWVGAMIVDVNQPILLITEPGKEQEAVTRLSRV
                     GFDHVLGYLNGGFATWEKSGKETDQINRITAAQFSKDYYDNSKVIDIRKESEYAAEHI
                     EDAYSKPLAYINEWIKDINPNEHFYMHCAGGYRSMIAASILQARGFRNFTEVEGGFKA
                     ISTTNIPKTNYICQSKILN"
     gene            2234..2602
                     /locus_tag="PLF48_07690"
     CDS             2234..2602
                     /locus_tag="PLF48_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF49242.1"
                     /translation="MSKQNFKLISYAIIALLVFVLFKNNFAQTDNTALKKAIKNGAFL
                     VDVRTPQEFSSGSVKGAVNIPLSEITQRISEFKGKKNIVVFCRSGNRSTQAKNILEQN
                     GIKNVINGGPWQNVNQIVNP"
     gene            2612..2800
                     /locus_tag="PLF48_07695"
     CDS             2612..2800
                     /locus_tag="PLF48_07695"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNF49243.1"
                     /translation="MQGLVKKYALIIIGIMAGAIGGYLYYKFVGCTSGTCAITSKPIN
                     STLYGAWMGGLLFSMFKK"
     gene            2838..3122
                     /locus_tag="PLF48_07700"
     CDS             2838..3122
                     /locus_tag="PLF48_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF49244.1"
                     /translation="MNVEKIVKENAGTIVDVRTPAEFMGGHVADSVNIPLQEIPYRMD
                     ELKNMKQPLVLCCASGNRSGQATYYLTQEGISCVNGGSWLSVNYLKSLAV"
     gene            3887..4357
                     /locus_tag="PLF48_07705"
     CDS             3887..4357
                     /locus_tag="PLF48_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNF49245.1"
                     /translation="MELYIKNVKDKGRGVFCTVDLNEGDIIEVCPVIVCPKKDCKHLD
                     KTHLYHYYFMWGNDGKSAGVVCGFGAMYNHAYNPNAIYETFYEDEIFRVKARRHIPAN
                     TEITINYNFYPDSQTPVWFDTEGLEKQAKEKAEKKKLKKADKKEKKKAKKEKKK"
     gene            4437..5687
                     /locus_tag="PLF48_07710"
     CDS             4437..5687
                     /locus_tag="PLF48_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF49246.1"
                     /translation="MAFLVDDPIENSQKNKVTLLIIVAALGYFVDVYDLVLFGVVRVS
                     SLKGIGITDDAALGVIGKMLFNWQMGGMLIGGILWGILGDKKGRLSVLFGSIFLYSLA
                     NILNGMITTVEQYKILRFLAGIGLAGELGAGVTLVSESMSREKRGYGTMIIASVGVFG
                     AVVASLIASKFNWRTSYFIGGGMGIVLLLMRIGVHESGMYANIKANTNVSKGNLLLLF
                     RNLTTFGKYISVILVGIPIWYTISSYIFFSKEMGQALHMAEIPNPAKAIMFNYIGLSF
                     GDLCSGYISQRLRSRNKTIFLFLCLSICAIAFYLFIGKNSINLFYTACTLIGFSAGYW
                     AVFNITATEQFGTNIRATVTTSVPNFVRGAVPVINVIIDIFQRAGQTYILATVYTGII
                     VFGLAFIVLKLMQDTFGKDLNYIE"
     gene            5759..>6205
                     /locus_tag="PLF48_07715"
     CDS             5759..>6205
                     /locus_tag="PLF48_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49247.1"
                     /translation="MKKSFVHVLFFTLLLMFSTSVFAQKTEKKTTDKSTSKMYKVDTK
                     KYLKDWLLVAHFGTTTPFTDIRSYDWVRQTKKPSELQWACGAGLTKMFTSAFGLNIDY
                     TLGKILGRTVDRGGFAEERQYWKVLGFDQPVYFKTTLFHQASVNFYI"
BASE COUNT         2082 a         1014 c         1195 g         1914 t
ORIGIN      
        1 aatttaaata taattttaac tataaatttt ggaagattta ctgcaaatag aggcctttaa
       61 atcgtctcct gctttagttg aaaaacttca agcatacagt attcggaaag aatataaagc
      121 gggaagcatc atactcaatg aaaatgtgca tattcgttct attcccatcg taactaaagg
      181 gacgctcaaa gttatccgta ccgaagatga cggcagagag atattactct attatatcaa
      241 agccggcgaa agctgcatca tgtcgtttct tggcggcatg cacaacgaaa caagcaaagt
      301 aagagctgaa gtggaagacc atgcagaaat actttttctg cccattgata aggtttctct
      361 ttttattaaa gaatacccac aatggctcga ttatattttt cgtttatatc acaagcgatt
      421 tgaagaattg cttgaaatca tcaatgcaat tgcgttcaaa aaagtggatg aacgcttgtt
      481 ggatttgttg caaaagaaaa aagaattaac aggaagcaat accctcatta tcacacacga
      541 acaactggct aacgaactag gcactgctcg tgtcgttgta tctcgtttac tgaaacagct
      601 ggaagaaata ggcaatgtaa aacttggcag aaataaagta actctccgtt aagcccgctc
      661 ctgtagtttc ttcggatcac attaaattgc catctaacta atgtctaaag acctctccac
      721 tattttcatt ctgaaaaaca ggcattaaaa agttgctttt ttaaaaaaaa atggcttgtg
      781 taactaatgt aacgcaattc gtgctcattc attcccaatt ttgtaacatc aaaatgtaaa
      841 ttataaaaat gaagatagaa caaatttata ccggatgttt ggcacaaggt gcttattaca
      901 tcacctctaa tggcgaagca gccatcattg atccattgcg cgaaacgcac ccttatctcg
      961 accgtttatc taaagacgga gtaaaattaa aatacatttt cgagacacat tttcatgctg
     1021 attttgtgag tggacattta gacttaagca aaaagacaaa tgcagcaatc gtgtacggac
     1081 cttctgcaca acctgaattt gaggccatta ttgccgaaga taatcaggct tttgagatag
     1141 gtaatattaa aataaaagtg ttgcatacac cgggacatac gatggaaagt acttgttttt
     1201 tgctgattga tgaaaatgga accgattcgg ctttatttag tggagatacg ctatttttag
     1261 gggatgtagg cagaccggat ttagcacaaa aagcggcaca tttaactcaa gacgaattag
     1321 ctggattgct ttatgaaagc atttacaata aaattttgcc cttagctgat gatattaccg
     1381 tttatccggc tcatggcgca gggtcggcat gcggtaaaaa tatgatgaaa caaacagtag
     1441 atacgcttgg caatcaaaaa ataatgaact atgcattaaa tcaaccgaac aaagaagcct
     1501 ttgtaaaagc tgttactgac ggattattac cccctcctgc ttatttcggt atgaatgtgg
     1561 cgatgaataa aaaaggatac gagcgtttcg atgatgtttt gcaacacagt ttaaaagcac
     1621 tgagtgccga cgaatttgaa gtggtggcag aaacaagcaa cgcattgatt ttagataccc
     1681 gcagtgacat agattttgct gtaggttata ttccgcagtc cataaatatt ggcttaaatg
     1741 gcgattttgc tccttgggta ggtgcaatga ttgtggatgt caaccaaccg atattattaa
     1801 tcacagaacc cggaaaagaa caagaggccg ttacgcgttt aagtcgtgtt ggattcgacc
     1861 atgtgttagg ctatttgaat ggaggatttg cgacatggga aaaatcgggt aaagaaactg
     1921 atcagataaa cagaatcact gctgcacaat tttcaaaaga ttattatgac aatagtaaag
     1981 tgattgacat aagaaaagaa agtgaatatg cagccgaaca tattgaagat gcttacagca
     2041 aacccttagc ttatatcaac gagtggataa aagatatcaa tcccaatgaa catttttata
     2101 tgcattgcgc tggcggttat cggagtatga ttgctgctag cattttacag gctagaggat
     2161 tcagaaactt tactgaagta gaaggaggat ttaaagctat ttcaacaacc aatattccta
     2221 aaacaaatta catatgtcaa agcaaaattt taaattaata agttatgcaa taattgcatt
     2281 actggtattt gttttattca aaaacaattt tgcacaaaca gataatacag ccttaaaaaa
     2341 agcgataaaa aacggggcat ttctggtaga tgtgcgcacc ccacaagagt tttcgagtgg
     2401 cagtgtaaag ggagccgtca atattccact gagtgaaatt actcaacgca tcagcgaatt
     2461 taaaggcaag aaaaatattg tagtattttg cagaagtggc aacagaagca ctcaggctaa
     2521 aaacatatta gaacaaaacg gaataaaaaa tgtaattaat ggcgggccct ggcaaaacgt
     2581 caatcaaata gtaaacccat aactcaactg aatgcaaggc ttggtaaaaa aatatgcact
     2641 gattatcatt ggaattatgg caggagcaat tggcggctat ctctactata agtttgtagg
     2701 atgcaccagt ggtacttgtg ctattacctc aaaaccaata aacagtacac tttatggtgc
     2761 atggatgggc ggattattat tcagtatgtt taaaaaatag aaagcaaaca ataaaaaaac
     2821 ggaataaaaa caaaaatatg aatgtagaaa aaattgtaaa agaaaatgca ggaacaatag
     2881 tggatgttcg tacgcctgcg gaatttatgg gtggacatgt tgcagattct gttaatattc
     2941 cattgcagga aataccctac agaatggatg aattaaaaaa catgaaacaa cctttggtgc
     3001 tttgttgtgc aagtggaaac agaagtggtc aagctactta ttatttgaca caagaaggca
     3061 tctcgtgtgt aaatggcggc agttggttga gtgttaatta cttgaaatct ttagctgtat
     3121 aaattgttga atttctgtta aagtccattt tattacagaa aacagtaaag aacacaaatt
     3181 accggggcgt atttaacaac gttgcaaaaa gattaaagca gtagcagtaa atgctgttaa
     3241 ctgcaaccac aaatacaaaa agagcatgca gtatgcttcc actaaattaa tacgtggaaa
     3301 catccggttt atattttaat taaaaagagt cggattaatt gatatggatt gagtagcttc
     3361 attcatcctt cactccataa caatatatat tgtatttata tctatattat atttagatat
     3421 gctttatagt aaaaccaaac attttcatca cacagatgct agcggcattt tgaaacatat
     3481 tcgtaactca tgtgctgttt atttttataa attttaccac atatagacat cgattttttt
     3541 ttaggattaa caacgttttc cttaaaaaaa tttagttacg catagcaaaa taaaggaatc
     3601 accaaagccg ttacattttt tttactgact atatttacta actcaaccaa aatgtaatat
     3661 atataattat tagttttata ctttttaagt gatttaagcc ccaaaaaaat tgcatttaac
     3721 acttgaactt aggtttaatg atacgatata gtatttaatt actttatcaa aatctatatc
     3781 tttgaatttt ttgacaactg ttctttgtaa caaaaacatt ttttttcgcc tatattctgc
     3841 tttgcttttg gataactagc tcgtatcttt gcacaaattt ttttcaatgg aattatatat
     3901 caaaaatgtg aaagacaaag gcagaggtgt tttttgtact gtagatttaa acgaaggcga
     3961 tattatcgaa gtatgtcctg ttattgtttg ccctaaaaaa gactgcaagc atctggataa
     4021 aactcattta taccactatt attttatgtg gggcaatgat ggcaaaagtg caggcgtggt
     4081 ttgcggtttc ggtgctatgt ataatcatgc atataatccc aatgccattt atgaaacatt
     4141 ttatgaagat gaaattttca gggtaaaggc acgcagacat attcccgcaa ataccgaaat
     4201 tacaatcaat tacaattttt atcccgattc gcaaacccca gtttggtttg acacggaagg
     4261 tctggaaaaa caagctaaag aaaaagccga aaagaaaaaa ttgaaaaagg ctgacaagaa
     4321 agagaagaaa aaagctaaaa aagaaaagaa aaaatagaat tttttttctt aattcattct
     4381 tgactaatta tgcttaattt cggtttactt ttttcacctt aacttcataa ttaagtatgg
     4441 catttttagt tgatgatccg atagaaaact cccagaagaa taaagtcaca ttacttatca
     4501 ttgtagccgc attgggttat tttgttgatg tatatgattt ggttctgttt ggcgttgtgc
     4561 gtgtaagcag cctcaagggc ataggaatca ctgatgatgc cgctttggga gtcatcggta
     4621 aaatgctgtt caactggcaa atgggcggta tgcttatcgg tggcatttta tggggcattt
     4681 taggtgataa aaaaggccga ctttccgttt tgtttggttc cattttttta tactctttag
     4741 ccaatattct gaacggcatg attaccacag tagagcaata taaaatcttg cgttttttag
     4801 ccggtattgg tcttgccgga gagctgggtg caggcgttac actggtaagt gaaagtatga
     4861 gccgcgagaa acgcggatac ggcaccatga tcattgccag tgttggtgtt tttggtgcgg
     4921 ttgtagccag tttgattgcc agcaaattca actggcgaac cagttatttc atcggtggcg
     4981 gaatgggaat agtattatta ttaatgcgca ttggggtgca cgagtcggga atgtatgcca
     5041 atattaaagc aaatacaaac gtatcgaaag gcaatttgtt gcttttgttt agaaacctga
     5101 ccacatttgg aaaatatatc agcgtgatct tagtgggtat tcccatttgg tacacgatta
     5161 gttcttatat ttttttctcc aaagagatgg ggcaagcttt acacatggct gaaattccta
     5221 atcctgcaaa agcaattatg ttcaattata ttggcttaag ctttggtgat ttgtgcagtg
     5281 gctatatcag tcagcgttta cgaagccgga ataaaaccat atttttattt ctttgtttgt
     5341 ctatttgcgc tatcgcattc tatttattta tcggcaaaaa cagcatcaac ttattttata
     5401 cggcatgtac ccttattggg ttttctgcag gttattgggc tgttttcaat attacggcaa
     5461 cagaacaatt cggtacaaat atcagagcta cagtaactac ttctgtgcct aattttgtgc
     5521 gtggtgcagt acccgtaatc aatgtaatta ttgatatttt tcagcgagcc ggacaaacct
     5581 atatccttgc aacagtttac actggcatca tagtatttgg tttggcgttt attgtattaa
     5641 aattaatgca ggatacgttt ggcaaagacc tcaattatat tgagtaataa attaatctta
     5701 tttttctgct ttgaatttaa aaagcatttt actatcttta ttcaaaattt agaatttaat
     5761 gaaaaagagt tttgtacacg tattattttt tacgctgttg ctgatgtttt caacatctgt
     5821 ctttgcacaa aaaacggaga aaaaaacaac agataaatct accagtaaaa tgtataaagt
     5881 ggataccaaa aaatacctca aagactggtt attggttgca cactttggaa cgacaactcc
     5941 atttacagat atcagaagtt atgactgggt gcgtcaaact aaaaaaccca gtgagttaca
     6001 atgggcatgt ggtgcgggac ttactaaaat gtttaccagt gcatttggct tgaatatcga
     6061 ctatactctt ggaaaaatat taggaagaac agttgatcga ggtggttttg ctgaagaaag
     6121 acaatattgg aaagttttgg ggtttgacca accggtatac ttcaaaacca ctctttttca
     6181 tcaggcatct gttaacttct atatt
//
LOCUS       DAOQII010000143         6104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7313_length_6104_cov_4.536452, whole genome shotgun sequence.
ACCESSION   DAOQII010000143 DAOQII010000000
VERSION     DAOQII010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7313_length_6104_cov_4.536452"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..920)
                     /locus_tag="PLF48_07720"
     CDS             complement(171..920)
                     /locus_tag="PLF48_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49248.1"
                     /translation="MPFNKIFAFYFCLVAFCTSQHIGHAQNSIDVVDPYQIKVDAAQQ
                     ANKADTFYFIYQGTKLNRKLDTLRTYDVFLEERLTPYGIAYMCNGKEVGKDRYYEMRR
                     FWRANGACLPCLLYTYDANDNLKYQAFQYEDCMCGEYKEFYEDGRKKVEGQFRPNTSG
                     NWEPDRLNGKCNIRIGKWTYFTPEQNTEKIEIYNDEGKLIDIKYAQSSINPKLNNKNS
                     NDEQQEEADGNEKKSVFKKLKNKIKQEDSNE"
     gene            complement(1066..3396)
                     /locus_tag="PLF48_07725"
     CDS             complement(1066..3396)
                     /locus_tag="PLF48_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49249.1"
                     /translation="MKFLNKSVIIFLIQAFLFFSVKAQEVEGGLVGETWMGGKKKAED
                     LYNKLAYTDAIPLYEALLKKKDDPKAQAQLGDCYRLTAQFEKAAAAYDKAVASGNKDA
                     STLLNQAQMHQTIQDYAGAKAAYEKYLNLNPNDKRAQNELNAINNLAQLNTPNMRYAI
                     ENLSINTRNFEFAPTYFGDGIYFASDRDQEKSINREHSWTGSTFYDMYFSKGEKTNMV
                     KPDNIKKEGQTKYHDAAATFTPDGKTVFFTRNNYFDNKLLKSGEKIVKQGIYSATVDG
                     KKWKDEKQFPFNNQEYDVAHPALSADGNTMIFSSNKPGGRGGMDLYRTTKTGESWSAP
                     ENLSELNTEGDEVFPSLDKEGNLFFSSNGLGGLGGLDLYRTTASSDYTNPVNLGAPLN
                     SSYDDFGLTYGKETAYGYFTSNRKEGKGEDDIWYFTDNGIFLEGIVVDAITKKPICSS
                     QVEMLTDAVSKGTKTTECDGLFNYDVDTNKDYCFKAKAENYLENNAVCASTKNVTPGS
                     TVKVEIPLQPIQPAGYTVMVVDKATKKEIKDAKVDISSNKDITKYEQKITGQDGKVCY
                     TVLCSYQYLANASAKGYLAGSNMFDTKGDCNNYVVCDKGEAQKVIVELERDPNAKDVT
                     TDENGNPISDANLSKELKDIYYDFDKWYLRKESEPQLNKLLKFLQENPDAIVEISSYT
                     DARATDEYNKVLSQKRAKSVVDWLIQRGINKNRLKPVGYGETKLRNECSDGVKCSEYE
                     HQRNRRTEFKIIGGKINIESLERFDMDVDPCAICPF"
     gene            complement(3423..4427)
                     /locus_tag="PLF48_07730"
     CDS             complement(3423..4427)
                     /locus_tag="PLF48_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF49250.1"
                     /translation="MNTINKLLSSILLLLTIGISTQTYAQQEPQYTQFMYNKLPINAG
                     YTGGRGCLSMRALYRTQWVKLEGAPQTASFSIHSPLKKENSALGFFFVNDRLGVTNQN
                     WFDVTYAYRIPVGKKHKVRIGLGINAGVFWYKSNLRELSPNNISDAVYNQNVNRILPD
                     IGAGLYVYHPMWYVGVSVPNFLKSSLYNKDQASLIASSPLAHRSQHFFAMAGGVIPAH
                     RAFKIRPQVMGKYVFAKGQKVPFEMDFNLSFLIIDRINVGATYRTTFGKVSNIDKLNN
                     PESVDVNLEVWPTKQLMIGYAYDYTLSKLGAYNKGSHEIILGYDFAFKNKKFDTPRFF
                     "
     gene            complement(4452..>6104)
                     /locus_tag="PLF48_07735"
     CDS             complement(4452..>6104)
                     /locus_tag="PLF48_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF49251.1"
                     /translation="STSTLVEVGANGIARTLGTLVPPVTSGNAHIMLGLIGTADNSGH
                     YLVAAAEGQVNLADTSVSNFKLYLGKVTVPDTTVAWTQVTLDASCTQFQQAFIDGIKN
                     GEDHGPQDMVWDPATGNVWLYSGVDKIFGVLNTTTNTGTCYADNNGISAVGNLGGLAL
                     DSVGQLIGLEVQTGNVYRIDTRGCMDGNPATPCITSLNLINNYTINGNNDTRGDAASC
                     VSACTPPSLTKQNFITCENNPVELSVNVSPSGSYTYTWGVYAGNGTLISATTATPTYS
                     NNTAGTYKVYVTIESATGCQKSDSIYVTVAKKPDTTLINKGLCANDSILFNGQIIKQT
                     GTYIALFTGFAGCDSLVKLIVTDSICNQNLPPVAVNNDTITYGGGVVIPVIFNDTDPD
                     GDDLTLCGGTGGIITPPQNGTVQVNIDGTITYTPTITSGIDSFQYAICDGKGGADTAW
                     VYITIEDCKIPNTFSPNGDGVNDFFEIPCVTGKEHVVLCVYNRWGAEVFRNENYDNVS
                     GLFEGKYKGEDLPDGTYYYVLKYTNAKNEKVDKAGFVVIHRK"
BASE COUNT         1736 a         1225 c         1052 g         2091 t
ORIGIN      
        1 aaatagtttc tttctcaagt tcatattaga caattttaac gcaatatata caaaatgatt
       61 aacagagagt acgctttttt ttaacattcg tttaaaattc accaaataca atgaaaaagc
      121 ccgccaaata attgacgggc tatggaataa ttcacatccc ccaatatgaa ctattcattt
      181 gaatcttctt gcttgatttt attttttagc tttttaaaaa cagatttttt ctcatttcca
      241 tcagcttctt cttgttgctc atcattactg tttttattgt ttaattttgg attgatactg
      301 ctttgcgcat attttatgtc aattagtttt ccttcatcat tatagatttc tattttttct
      361 gtgttttgtt ccggcgtaaa atatgtccat ttgccaatgc ggatattgca ttttccatta
      421 agtctgtctg gctcccagtt tccactggta ttgggtctga attgtccttc tacttttttt
      481 ctgccatctt cataaaactc cttatactcg ccacacatgc aatcttcgta ctgaaaggcc
      541 tgatatttta aattgtcatt ggcatcgtag gtatataaca aacaaggcag gcaagctcca
      601 ttggctctcc aaaatcgtcg catttcgtaa tagcggtctt tgccaacttc tttaccatta
      661 cacatgtagg caataccata aggggttagt cgttcttcta aaaatacatc ataagtacga
      721 agcgtatcta atttacggtt cagtttagtg ccctgataaa tgaaataaaa ggtatccgct
      781 ttattcgctt gttgagcagc atccacttta atttgatagg ggtcaacaac atcaatacta
      841 ttttgagcat gcccgatatg ttgacttgta caaaaagcta ctagacagaa ataaaaagcg
      901 aatattttat taaacggcat gacctaaaga tatcagattt caaacagtca tttttacacg
      961 atatataaac agatgtgaat taactcaaat atcagcaaaa gttgtggact ttaaataaaa
     1021 agaatgctcc cctattggag agcattcttt gtagttagtt atatgttaga aaggacaaat
     1081 tgcacaagga tcgacatcca tatcaaatcg ctcaagtgat tctatattga tttttccgcc
     1141 tataatttta aactccgttc ttcggttgcg ctgatgttcg tattcactgc attttacgcc
     1201 atcagagcat tcgtttctca atttagtttc gccataacca acaggtttta gtctgttttt
     1261 attaatacct cgctgaatca gccagtccac tacactttta gctcgttttt gagaaagtac
     1321 tttgttgtac tcatcagtag ctcttgcatc tgtataggaa gatatttcaa caattgcatc
     1381 cggattttcc tgtaagaact tcaaaagttt gttcaattga ggctcacttt cttttctcaa
     1441 ataccattta tcgaaatcgt aatagatatc ttttaattct tttgacagat tggcatcaga
     1501 aattgggttt ccgttttcat cagtggttac atctttagca ttcggatcac gctcaagttc
     1561 aacgattact ttttgtgctt cgcctttatc acaaaccaca taattattgc agtcaccttt
     1621 ggtatcaaac atattgcttc cggccaaata accttttgca ctggcatttg ctaaatattg
     1681 ataactgcac aaaacagtat agcatacttt gccatcctgg cctgtaattt tttgttcgta
     1741 tttagtgatg tctttattag aactgatatc aacttttgcg tctttaattt cttttttagt
     1801 cgctttgtca accaccatta ctgtatatcc tgccggttgg atgggttgta aagggatttc
     1861 aacttttact gtagaacccg gggtcacatt tttagtcgat gcacaaactg cattattttc
     1921 cagataattt tctgcttttg ctttgaagca atagtcttta ttggtatcta catcgtaatt
     1981 aaacaaacca tcacattcgg ttgtctttgt tcctttagaa acagcatcag taagcatttc
     2041 tacctgactg ctgcaaattg gttttttagt aattgcatct acaacaatac cttccaagaa
     2101 aatgccgttg tctgtaaaat accaaatatc atcttcacct ttcccttctt ttcggttaga
     2161 ggtgaaatat ccataagccg tttcttttcc ataagttaat ccaaaatcat catatgaaga
     2221 attgagcgga gcgcccaaat ttacaggatt agtataatct gatgaagcag tagtacgata
     2281 taaatctaat cctcctaatc caccaagacc attgctggaa aagaataaat taccttcttt
     2341 atctaaactg ggaaatactt cgtcgccttc agtattcaat tcacttaaat tttcaggtgc
     2401 tgaccaactc tctccggttt ttgtggtgcg gtataaatcc atgcctcctc ttcctccggg
     2461 tttattgctc gaaaaaatca tcgtattacc atcagcactt agagcaggat gagcaacatc
     2521 atattcctga ttgttaaaag gaaactgttt ttcgtctttc cattttttac catcaactgt
     2581 agcagaatag ataccttgct taacgatttt ctcaccgctt tttaaaagtt tattatcaaa
     2641 atagttgttt ctggtaaaaa atacggtttt gccatccggc gtaaaagtag ctgcggcatc
     2701 atgatatttg gtttgtcctt cttttttaat attgtccggt tttaccatat ttgttttttc
     2761 acccttagag aagtacatat cgtaaaaagt agatccggtc cagctatgtt ctctgttaat
     2821 agatttttcc tgatctctgt cggatgcaaa ataaatgcca tcgccaaaat aagtgggagc
     2881 aaattcaaaa ttacgtgtat ttatagataa gttttcgata gcgtagcgca tgttcggcgt
     2941 atttaattgc gctaaattgt ttatcgcatt cagttcattt tgtgcacgct tgtcgtttgg
     3001 attcagattc agatattttt catacgcagc ttttgcacct gcataatctt gaatagtctg
     3061 atgcatttgt gcctgattta atagggtgga agcatctttg tttccgcttg ctacagcttt
     3121 gtcataagct gcagcagctt tttcaaattg tgcagtaagc cggtaacagt ctcccaattg
     3181 agcttgtgct ttcggatcat cttttttctt caagagtgct tcatataaag gaatagcatc
     3241 agtataggct aatttgttgt aaaggtcttc tgcctttttc tttccgccca tccatgtttc
     3301 gcctacaagt ccgccttcca cttcctgagc cttaacagaa aagaataaga atgcctgtat
     3361 caaaaagatt ataacagatt tattaagaaa tttcatacaa taatttttac gtgtttatgg
     3421 aattaaaaga atctgggtgt atcgaatttt ttattcttga atgcaaaatc ataacccaaa
     3481 ataatttcgt gcgaaccttt gttgtaagcc cctagttttg ataatgtgta atcgtaagca
     3541 taacctatca tcagttgctt ggttggccac acttccagat ttacatctac actttcagga
     3601 ttattcagct tatcaatatt gcttacttta ccaaaagtgg ttctgtatgt tgcacctaca
     3661 ttgattctgt caatgatgag aaagctaaga ttgaaatcca tctcaaatgg tactttttgt
     3721 ccttttgcaa acacatattt tcccatcact tgtggtctga ttttaaaggc acgatgtgca
     3781 ggaataacac ctcctgccat tgcaaagaaa tgttgtgaac ggtgtgccaa tgggctggac
     3841 gcaatcaaac ttgcctgatc tttattatat agactgcttt tcaggaaatt tgggacactt
     3901 actcctacat accacatggg gtgatataca tataaaccgg ccccgatatc aggcaggatt
     3961 ctgtttacgt tttgattgta aacggcatct gagatattat ttggtgataa ttctctcagg
     4021 ttgcttttat accaaaacac acctgcattg atacctaatc cgatgcgtac tttatgtttt
     4081 ttgcctacag gtatgcgata cgcataggta acatcaaacc agttttggtt agtaacaccc
     4141 agtctgtcat tgacaaagaa aaagcctaat gcgctatttt ctttctttag cggactgtga
     4201 atggaaaatg aggctgtttg cggagcacct tcgagtttaa cccattgtgt acggtatagc
     4261 gcacgcatgc ttaaacatcc tctaccgccg gtatatccgg cattaattgg caatttatta
     4321 tacataaact gcgtgtattg tggctcttgt tgtgcatatg tttgtgtaga aatgccaatg
     4381 gtaagaagca acaatattga tgataataat ttatttatag tattcataac tttattactt
     4441 tatgcggtga attatttacg gtgaatgaca acaaatcctg ctttatcaac tttttcattt
     4501 ttagcatttg tgtatttaag tacataataa taggtgccgt ctggtaaatc ctcaccttta
     4561 tatttacctt caaacaatcc gcttacattg tcatagtttt catttctgaa tacctctgca
     4621 ccccagcgat tatacacaca taatactaca tgctctttac ccgtaacaca tggtatttca
     4681 aagaaatcat ttacgccatc tccattaggg gagaacgtat ttggaatttt acagtcttca
     4741 atagtaatat agacccaagc cgtatctgca ccgcctttgc catcgcaaat agcatattgg
     4801 aaactgtcaa tacctgatgt gattgtaggc gtgtaagtta tagtaccatc tatattcact
     4861 tgaactgtac cattttgtgg aggagtaata atgcctcctg taccaccgca caaggttaag
     4921 tcatcgccat ctgggtcagt atcattaaat attaccggta taactactcc gccgccataa
     4981 gtgatggtgt cattatttac agcaaccggc ggaaggtttt gattgcagat tgaatctgtt
     5041 acaataagtt ttactaaact gtcgcaaccc gcaaagcctg taaacagtgc gatgtaagtt
     5101 ccagtttgtt ttataatctg tccattaaat aagatactgt catttgcaca aagccctttg
     5161 ttgatcaaag tggtgtccgg ttttttagca actgtaacat atattgaatc cgatttttga
     5221 cagcctgttg cactctcaat ggttacatat actttataag tacctgcagt attatttgaa
     5281 tatgtcggtg ttgccgtagt agcactgatc aatgtaccat tgcctgcata aactccccaa
     5341 gtataagtat agctgccact aggggaaaca tttacagaaa gttcaaccgg attattttcg
     5401 caggtgatga aattttgttt agttaatgac ggtggtgtac atgctgatac gcaactggcc
     5461 gcatcaccgc gtgtatcatt attaccatta atagtatagt tgttaattaa attgagggaa
     5521 gtgatacaag gcgtagccgg attgccgtcc atacaaccgc gagtgtcaat tctgtagaca
     5581 tttccggttt gtacttccag cccaattagt tgtccaacgc tatccaacgc taaaccgcct
     5641 aagttaccta cagcactaat gccgttatta tcagcataac aagttccggt attagtagtg
     5701 gtatttaata caccaaatat tttatctaca ccggagtaca gccaaacatt acctgtagcc
     5761 ggatcccata ccatatcctg aggaccatgg tcttcaccgt ttttaatgcc gtcgataaat
     5821 gcttgttgga attgtgtaca agaagcatct aaagtcactt gtgtccatgc tacggtagta
     5881 tccggcactg taactttacc aagataaagt ttaaagttgg aaacgctggt atcggctaaa
     5941 ttaacctgac cttcggcagc agccactaaa tagtgaccac tattgtctgc tgttccaatc
     6001 aagcccaaca taatatgagc attaccggaa gtaaccggag gaaccaatgt gccgagagta
     6061 cgagcgatcc cgttagcacc tacctctact aaagtagaag tgga
//
LOCUS       DAOQII010000144         6090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7331_length_6090_cov_5.730406, whole genome shotgun sequence.
ACCESSION   DAOQII010000144 DAOQII010000000
VERSION     DAOQII010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7331_length_6090_cov_5.730406"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLF48_07740"
     CDS             complement(<1..890)
                     /locus_tag="PLF48_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNF49252.1"
                     /translation="MTNYPSPLERFYHWEATQPNKTFLRQAYKGQWLHTSWSSAGDEI
                     RRMAAYLQSLNLPPQSNIAIISKNCANWIMSDMAIMMAGHVSVPLYPTIPAKDIRYCL
                     EHSEAKVCFVGKLDDWEKQRSGVPDDVICINFPMWTETGTNYVYWEDIIRKYEPITTN
                     YLPDYNDLITIIYTSGTTGLPKGVVHRYHAFGFAATNALDVIGGLDTSERFFSYLPLS
                     HIAERMLVEMGGIYTGGSLSFAESLDTFPANLKDTQPTIFLAVPRIWTKFQMGILNKM
                     PQSRLDLLMKIPIVSTLIKH"
     gene            complement(1012..1458)
                     /locus_tag="PLF48_07745"
     CDS             complement(1012..1458)
                     /locus_tag="PLF48_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HNF49253.1"
                     /translation="MKALLKTQFGHPKQPTFFDDFFGKDFFGYSPASVKTMPAVNVKE
                     DETNFTVEVAAPGMNKDNFNISLEDGILTISSESKSENTETDDKTKYTRKEFSYSSFK
                     RSFTIDEEQINTEAISARYENGVLFVALPKQEKVEPEKKSKTISIS"
     gene            complement(1756..3150)
                     /locus_tag="PLF48_07750"
     CDS             complement(1756..3150)
                     /locus_tag="PLF48_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49254.1"
                     /translation="MKNTLKLLLSFGIFLTGILQMNAQNCNRYVDGDSAKTTAPYSIY
                     REFFKKNLISEAYPYWKQIYTKAPLFRLQTMMDGEAIYTKFIQDTEDKSLKEKYADTL
                     FALYDKRIQCFGQEDYVLGKKAIDALKYKGNAAIPDARAWFEKSLKISGATPYPFVVQ
                     TYFKLLVNQVGNGDITTDLVKGKYDELSVLVDKGIAKNDKNSAAYKEVKTLMDEMYAQ
                     NFADKSDPADCAKLMEIYKKRYNEKPNDLENIRTVYNKVRGCNDSLFNVELLKKLNNL
                     EPSYTYAIRLGNLYMKNKKFDSAYILYQNAVVKEADSAKKADLYYIMASLKADKDDFP
                     AARDLAKQALQYSPTMGKASLLIGTLYAASGKMCGPGVGFQSQIVLWPAFDYFKKAKE
                     DPTTADEAQKMISSYTKYLPTTGDIKTKGLSQGQTYKIGCWINETTTVQAIAGPANSA
                     NAPKTKTSTKTKSK"
     gene            complement(3165..4553)
                     /locus_tag="PLF48_07755"
     CDS             complement(3165..4553)
                     /locus_tag="PLF48_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49255.1"
                     /translation="MRSSLLLIFTLIVLAAYGQDLTPYSRYGLGFAADNNHAQSAQMG
                     GLGAAFQSSESPNYLNPASYGAMVLTTFDAGFGGNFIRLKTNTMKAKQNNFALNYLSI
                     FVPVKKYWVTGVGLLPVSVKDYFVTQTSILDTNYSAQFEYEGSGNLYNAFWGNGFKYK
                     GLYLGFNFGYMFGKLNNNTFTYLVDPSGSYDPYTNTAWSFSNVKIGSLTYNLGAQYRL
                     DIKTKKDSSLYMVFGISGSSPLKVLKNSTIDNAAYAFSSTQLGYRSSDQGINDFLTDY
                     LIPKASYSDIMDTLKEQFGAKASIKLPGHINFGVIAGKSNRWKAGFDFKYQFWSKYQG
                     YESGAVSKLSNSFRIGLGGEYFPLGKGKTNFFKTLKYRAGFYYTRTNITIGNQSINEF
                     GTSFGIGIPLIIRVGNNEGLMEKYLVHPFNIGIEAGSRGTVRNNLIQETFIRLKFGVS
                     LNDRWFVKRKYY"
     gene            complement(4543..5265)
                     /locus_tag="PLF48_07760"
     CDS             complement(4543..5265)
                     /locus_tag="PLF48_07760"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNF49256.1"
                     /translation="MNLAVDIGNTRTKIGIFDGMVQKETLVFNHNQEIDFYSIITTYH
                     IRSAILSSTVEIPASYFNIAELLEQSFVLNKHTLLPFKNEYSSKDTLGNDRLALVAAA
                     QAQFPHQHNLVIGCGTCITFNFLNANDEFLGGSIHPGLKMRFKAMHHFTGKLPLAEIT
                     PDVGLVAANTISNLQTGVVYAAAKEIDGMIDEYLVKFPELNVIITGGDAGVLVNRLKN
                     KIFAISDFTLIGLNYILLYNAK"
     gene            complement(5265..>6090)
                     /locus_tag="PLF48_07765"
     CDS             complement(5265..>6090)
                     /locus_tag="PLF48_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF49257.1"
                     /translation="CNTTDTFYQKQKENLFYYSFVEYFDLFQTDNTKQTIAQYNAVYD
                     ALSETNKNKKCKVPHAPYSVSEELFEYLTQTESGKTMSIHNQETQAEQDLFQGKRGSF
                     ISFYDRMNLATRAIPYRNSPSINYALEYLSKNNRNLFVHNTLTTKEDILLTFDVLEKE
                     RTFWCTCPNANLYIENKLPDYRAFLAADATLCIGTDSLTSNWQLNILEEMKTILKYCS
                     YLQFEEVLKWATLNGAKALGADTVYGSFETGKTPGVVHISGVENQDISKAVSKRIY"
BASE COUNT         1790 a         1170 c         1095 g         2035 t
ORIGIN      
        1 ttatgtttga taagcgtact tacgatagga attttcataa gtaaatcaag acgactttgt
       61 ggcattttgt ttagaattcc catttgaaac ttggtccaga tgcgcggaac ggctaaaaat
      121 atagtaggtt gcgtgtcttt cagatttgcc ggaaaagtat ctaatgactc ggcaaatgaa
      181 agtgaacctc ctgtataaat accacccatt tcgaccagca tacgctctgc aatatgcgat
      241 aaaggcaaat aagaaaaaaa gcgctcggag gtatccagtc caccaattac atccagtgca
      301 ttggtagctg caaatccgaa agcatgatag cggtgcacca ctcccttggg taatcctgta
      361 gtacccgatg tataaatgat agtaatcaga tcgttataat caggaagata attagttgta
      421 atcggttcgt attttctaat aatatcttcc caataaacat agttcgttcc ggtttccgtc
      481 cacataggga aattgataca aattacatca tccggcacac cactgcgttg cttttcccaa
      541 tcatccagct tgccgacaaa acagacttta gcctcactgt gttctaggca ataacgaata
      601 tctttagccg gaatagtagg ataaagcgga acagaaacat gtcctgccat catgatagcc
      661 atatcgctca ttatccaatt tgcacagttt ttagaaatga tggcaatatt tgattgtggc
      721 ggcaaattta gagactggag atatgcagcc atcctgcgta tttcatcgcc ggcactgctc
      781 catgaggtat gcagccattg ccctttataa gcctgccgga gaaatgtctt attgggttgt
      841 gtagcttccc aatgataaaa acgctctagt ggagatggat agttggtcat agtcatattt
      901 tatgcagata atttctaaaa ttaaaaattt atcgcattaa tacaaggttt ttatacaata
      961 aaaaaaatcc cggcacaagt ccgggatcaa ctaactaact attctatgtc tttatgaaat
     1021 agaaattgtt tttgatttct tttccggttc taccttttct tgttttggca atgctacaaa
     1081 caacaccccg ttttcgtatc gggcactaat tgcttcggtg ttgatttgtt cttcatcaat
     1141 cgtaaaactg cgtttgaatg aagagtagga aaactcttta cgggtatatt ttgttttatc
     1201 gtccgtttct gtgttttcac ttttgctttc agaggagatg gttaaaattc cgtcttccag
     1261 actgatgtta aagttatctt tattcattcc gggtgcggct acttcaaccg taaagtttgt
     1321 ttcatcttct tttacattta ccgccggcat agttttaact gaagccgggc tgtaaccgaa
     1381 gaaatctttt ccaaagaaat catcaaagaa ggtgggttgt ttgggatggc caaattgtgt
     1441 ttttaataat gctttcattt tatttgagtt tagttgttaa tttaatttac aagctattaa
     1501 tcaaactaaa taccaataca caaatgctga tgaatttgct gaatacacaa ataaattacg
     1561 tcattttgtc tgtttttatt attaaaacat gacacaaatt ccgtttttta tgtctattaa
     1621 tagaataaca acctaaagtt gggttagaaa ataaagtgat aagatagctg atttctactt
     1681 tttataagga attggttgtt gtaaataaaa aaacggctca ccttcatgaa ccgttttttt
     1741 tataggcttt gctgattatt ttgatttagt tttagtgctt gtcttagttt taggagcatt
     1801 ggcagaattt gccggtcccg caatagcctg caccgttgtt gtttcattta tccaacatcc
     1861 tattttataa gtttgacctt gcgacaagcc ttttgttttt atatcgcctg tggttggtaa
     1921 atatttagtg tatgatgaaa tcatcttttg tgcctcatct gcagtagttg ggtcttcttt
     1981 agcttttttg aaataatcaa aagccggcca taatacaatt tgtgattgga agccaacgcc
     2041 aggaccacac attttgccgc tagcggcata tagtgtgcca attaacaaac tggctttacc
     2101 catggttggg ctatattgca atgcttgttt tgctaaatca cgagctgccg gaaaatcatc
     2161 tttatctgct ttcaaagagg ccatgatata atataaatct gcttttttag ctgaatcagc
     2221 ttcttttacc actgcatttt gatataagat gtatgctgaa tcaaatttct tatttttcat
     2281 atacaaatta cctaaacgaa tagcatacgt atagcttggt tctaagttgt ttagtttttt
     2341 cagcaattct acattaaaca aactgtcatt acagccgcga actttattgt agactgttct
     2401 gatgttttcc aaatcattcg gtttctcgtt gtatcttttc ttataaatct ccatcaattt
     2461 agcacagtct gccggatcgc ttttatcagc aaaattctgt gcgtacattt catccattaa
     2521 tgttttcact tctttataag cagcactgtt cttgtcattt tttgctatgc ctttatcaac
     2581 caacacagac aactcatcgt attttccttt tacaaggtcg gtggtaatat caccgtttcc
     2641 aacttgattt actaagagtt taaaataagt ctgaacaaca aatggatatg gagtagctcc
     2701 actgattttc aatgatttct caaaccaagc acgagcatcc ggaatagcgg catttccttt
     2761 atatttcaat gcatcaattg cttttttacc tagtacataa tcttcttgtc cgaagcattg
     2821 tatgcgttta tcgtacaagg caaataatgt atctgcatat ttttctttca gagatttatc
     2881 ttctgtatcc tgaatgaatt tggtataaat ggcttccccg tccatcatgg tttgcaaacg
     2941 gaagagaggt gcttttgtat aaatctgttt ccaataagga taagcttctg aaatcaaatt
     3001 cttcttaaaa aattctcgat aaatcgaata gggcgctgtg gttttagcac tgtcaccgtc
     3061 aacataacga ttgcaatttt gtgcattcat ctgtaaaatg ccagtcagaa agattccgaa
     3121 actcaatagt aattttagcg tgttcttcat tttgttcaaa ttttttaata gtattttctt
     3181 tttacaaacc atctgtcgtt tagagaaaca ccaaatttaa gtcgaatgaa agtttcctgt
     3241 atcaggttgt ttcgtactgt tccgcggctt ccggcttcaa ttccgatatt aaacggatgc
     3301 acgagatatt tttccattaa cccttcgttg tttcctaccc gaatgattag tggtatacct
     3361 attccaaaag acgtgccaaa ttcgttaatt gattgattac caatggtaat attagttcgg
     3421 gtatagtaga atccggcacg atacttaagt gttttaaaga aattggtctt tcctttaccc
     3481 agtgggaaat actctccgcc taagccaatt ctgaagctgt tgctcaattt ggacactgct
     3541 cccgattcat atccctgata tttactccaa aactgatatt taaaatcaaa accggctttc
     3601 catcggttgc ttttgccggc aattacacca aaattgatat gtccgggtaa ctttatactg
     3661 gctttagcac caaattgttc tttaagcgta tccatgatat cggaatagct cgctttggga
     3721 atcagataat ctgtcaaaaa atcattgatg ccctgatcgg aagaacggta gcctaattgt
     3781 gtagaagaaa atgcataagc ggcattgtca attgttgagt ttttcagcac ttttaacggg
     3841 gaggaacccg aaataccaaa taccatgtac aggcttgagt ctttcttggt ttttatatcc
     3901 aaacgatatt gtgctcccaa attataggta agactgccga ttttaacatt ggaaaaactc
     3961 catgccgtgt ttgtgtaggg gtcgtagctt cctgacggat ctactaaata agtgaaggta
     4021 ttattattca acttaccgaa catatagccg aaattaaaac cgagataaag ccctttgtat
     4081 ttaaagccgt taccccaaaa tgcattatac agattacccg agccttcata ttcaaactga
     4141 gcggaatagt tggtatctaa gatggaagtt tgtgttacaa aataatcttt aacacttacc
     4201 ggcaataaac caactccagt aacccagtat ttttttaccg gcacgaatat cgacaaataa
     4261 ttcaaagcga aattattctg ctttgctttc attgtattgg ttttcaggcg aataaagttt
     4321 ccgccgaaac cagcatcaaa ggtagtcaat accatagcac catacgaggc cggattcagg
     4381 taattggggc tctctgaaga ttggaatgca gcccccaagc cacccatttg tgccgactgt
     4441 gcatgattat tatctgcagc aaaccccaag ccgtagcggg aatatggagt taaatcctga
     4501 ccataagccg caagtacaat cagtgtaaaa attaataaaa gactacttcg cattgtataa
     4561 taaaatataa ttcaagccga ttaatgtaaa atcggaaatt gcaaatatct tatttttaag
     4621 ccgatttacc aatacaccgg catcaccacc agtaattatt acattcaatt caggaaattt
     4681 aactaaatat tcgtctatca tgccgtcaat ttctttggca gcggcatata caacacctgt
     4741 ctgcagatta gagattgtat tcgctgcaac taaaccaaca tccggtgtaa tttcagccaa
     4801 aggcaattta cccgtaaaat ggtgcatggc tttaaagcgc attttcaagc ccgggtgtat
     4861 actgccccct aaaaattcat cattggcatt aaggaaatta aacgtgatac aagtaccaca
     4921 acctatcacc aaattatgct gatgtggaaa ttgagcctga gcggcagcaa ctaaagccag
     4981 cctgtcgttg cccagtgtgt cttttgatga atattcgttt ttaaagggca ataatgtatg
     5041 tttattgagt acaaaggatt gctccagtaa ctcagcaata ttaaaatagg atgccggaat
     5101 ttctaccgta gaggaaagta ttgcactccg gatatggtat gtggtgataa tgctgtaaaa
     5161 atctatttct tggttatgat taaaaactaa agtttctttt tgcaccatgc catcaaaaat
     5221 gccgattttt gtacgcgtat tgcctatatc aactgccaga ttcatcaata aattctttta
     5281 cttacagcct tactaatatc ttgattttcc accccggaaa tatgcactac gccgggtgtt
     5341 tttcctgttt caaatgagcc atatactgta tctgcaccta atgcctttgc cccatttaga
     5401 gtagcccatt tcaacacctc ttcaaattgc aggtaagaac agtattttaa gatagttttc
     5461 atttcttcca gtatattcaa ttgccagttt gaggtgaggc tgtctgtgcc tatacacagt
     5521 gtggcgtctg cagctaaaaa cgcacgatag tcaggtagct tgttttctat atacaaattg
     5581 gcattggggc aggtacacca gaaagtcctt tctttttcca atacgtcaaa agtcagtaaa
     5641 atatcttctt ttgtagtcag tgtattatgt acgaatagat tgcggttatt tttactcaga
     5701 tattcaagtg cataatttat agatggtgaa tttcggtagg gtatagcacg ggttgccaag
     5761 ttcatgcgat cataaaacga gataaacgaa cctcttttac cctgaaataa atcttgctca
     5821 gcctgagttt cttgattatg aatcgacatc gttttcccag attcggtttg tgtcagatat
     5881 tcaaacaatt cttctgagac cgaataaggc gcatgcggta ctttgcattt tttgtttttg
     5941 tttgtttctg ataatgcatc atatactgca ttgtactgtg cgatagtttg ctttgtatta
     6001 tctgtttgga acaaatcaaa atactctaca aacgaataat agaaaagatt ttctttttgc
     6061 ttctgataaa aagtatcggt agtattacag
//
LOCUS       DAOQII010000145         6080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7349_length_6080_cov_5.760830, whole genome shotgun sequence.
ACCESSION   DAOQII010000145 DAOQII010000000
VERSION     DAOQII010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7349_length_6080_cov_5.760830"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..3204)
                     /locus_tag="PLF48_07770"
     CDS             complement(82..3204)
                     /locus_tag="PLF48_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF49258.1"
                     /translation="MSITEIAVKRPLLISVVFLVLILFGIMGYSKLNYNLLPKFEAPI
                     LSIQTIYKGASSEEVQNSVTKKIEDAASSIEGVDIVSSSSMENVSTVIVQLKQKTNTT
                     LAQQDVERKINQIKNDLPQEADEPVINKFSSSEIPVLRLTTFANMNDAELYDFVNLTV
                     KPLLLNLQGVGQVRLIGGIKREIEVKLNDDKLKAYSISSAQIYQTLAGSNMSYPAGNI
                     EDKELRYTIRLDEKFKSIDEIRNLIVRENRMGSRVTLGDVAMITDATSTPTSINRING
                     RVGLGMEISKQTDANAVEVSSLVKAKLDTIVKDYNTKGFKYDIAVDQSVYTLEAATSV
                     IHDLLLAVVIVALVMLFFLHSFRSSFFVLVALPSAMIPTFGLMYIMGFSLNLMTLLAL
                     SLVVGILVDDSIVILENIFRHMEMGKDRMKASLEGRSEIAFTALAITLVDVVVFVPLS
                     LAGGLIGNILREFALVVVFSTLMSLFVSFTLTPLLASRYAELVHLNEHSLWGKINLGF
                     EKFITNLTNNYGKLLAWALSHKRYVIIMVLALFGLIGFLGASGFIGSSFAGNGDRGEF
                     SLMIELEPQATLMQTNRVVQDVEKIILQYPEVKKVFSKVGAVGTQMGTTIESPNQADI
                     TVGLTDKDKRSISTDQLCNILRPRIDSIPGITFSIKPTNVTGNNQPQIQIVVMSPNDD
                     EMWAAAKKVKDIVYKTPGADYVEFSTKSAKTEIKISLDRDKIAKMGLSIPAVGNAIQL
                     AFRGNDQSKFKDKGEEYPINIVYEKSDKQSVTDIANTMLQTATGAVVRLGDVASINEV
                     QGQAILERYNRLNSVQILASTTGRPTGTVTAEIQEKIAKAGLPSTVKIDYLGEEKNQK
                     ESFGSLGMAMGIGILLVYLIMVALYESTLYPFVVMFSIPVALIGSVLALALTMESLSI
                     FGFIGFIMLMGLVAKNGILIVDFTNHLKARGMHLKEALIEAGRERLRPIMMTTFAMVF
                     GMLPIALSNGPGSEFKRSMAWVIIGGLTSSLLLTLFVVPTVYYLFDRLKEKISKKKDK
                     ADDTYLEMYS"
     gene            complement(3206..4261)
                     /locus_tag="PLF48_07775"
     CDS             complement(3206..4261)
                     /locus_tag="PLF48_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF49259.1"
                     /translation="MKSKITAGIILVAITALIAYKLTQNKKVIDKNNTPTDRTHVPVS
                     VSTFDAQYLPISTDYALPAVLDINKSGIITATQPGKLAAFNVEIGQHVSKGQLIGKID
                     SKQREIGIKSTDVTIKKLQDDLKRTQDLIAGDAAPANAVNDINYNIETSKIQKENLQQ
                     QIADNNIYAPISGIITQKNANAGEFANPGVPLATIMDVSVLKAVVFVSENNVYDLKTG
                     QAAQISSVVFPGKLTRGVIKYIAPKGDENHNYRVEVEIPNSGYKAGTYVSVKFAFKKP
                     AEALQIPKMALVEGTKNPYVYVVSGNTVQVRKLELGEENGQNIVVRSGLVPGEKVITS
                     GLININENSKVQIINNK"
     gene            complement(4268..5605)
                     /locus_tag="PLF48_07780"
     CDS             complement(4268..5605)
                     /locus_tag="PLF48_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF49260.1"
                     /translation="MKDVKYLLIALFITLFFTVNTKAQTQYNLQQCIAYTLKYNPSVI
                     MSNNDVDIAYARKREGQSAYLPQVYGQLKWDDNLKLQSTQFPSMSLGGITIPEQTLQI
                     GNKYNTAATLQLEQMIFNWSYIQGIKAIQPGYEIAKMKKEKTQQDLVYNTIAAYYTIL
                     LINENEKLIFQNEERLSKTISILKLQKEKGVIRQVDVDKVQVSLNTILSQEEILAANK
                     SVAFNNLKYAMGMPLDSIIAIDTVYNKEVIDLKTYSDTANVRNRIELQLMRKNIQLQG
                     ILQKRITAGYMPQISFYARYGANAFGSKFSKSFTNWHDFSALGFSVDIPIFDGLRTSS
                     SLRQSKLNIANLKEDLKQKEEGLKLQLINAQAQMDNAVENLNINKANYEMARNILDVT
                     TLQYQKGVISYTDWIGAEYAFKEAEQNYFTAMVKYIQSKLDVDRANNNIYIYK"
     gene            complement(5605..>6080)
                     /locus_tag="PLF48_07785"
     CDS             complement(5605..>6080)
                     /locus_tag="PLF48_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49261.1"
                     /translation="PNKADLVHFCVAMDLKEDEREIHAISEKTDNAIEELLQIGLHIQ
                     EELKIFHPAIINDLMKFFPESWTLVENHRDVFAHKNIARNIKKGIKQGYFRKEISIEI
                     TTLIQLQLSFLPLQPIQSEHKPTDIHLEVLKYNIYAIATPAGIELFQKLIKKMKL"
BASE COUNT         1706 a         1197 c         1097 g         2080 t
ORIGIN      
        1 cttcgtcatt ggaatgacgt acttaaaatt aagggattcc tctcttcgtt cggaatgacg
       61 tgtatttttc taactcgtaa attaactgta catttccaaa taggtgtcat cagctttgtc
      121 ttttttcttg ctgattttct ctttcaaacg atcgaataaa taatatacgg tgggtaccac
      181 aaacaaggta agcaatagtg aactggttaa tcctcctata attacccatg ccattgaacg
      241 tttaaattca gaacccggtc cgttagataa agcaatcggc aacataccga ataccatcgc
      301 aaacgtagtc atcataatgg ggcggagacg ctctcttcca gcttctatta atgcttcttt
      361 caaatgcata cctcgcgctt tcagatgatt cgtaaagtcc acaatcaaaa taccgttttt
      421 agcaaccaaa cccattaaca tgataaatcc gataaatccg aaaatactca aactctccat
      481 cgtcagtgct aaagccagaa cagagccaat caatgcaacc ggtatagaga acataactac
      541 aaacggataa agcgtgcttt catacaatgc aaccatgatt aaataaacca ataagatacc
      601 aatacccata gccataccta agctgccaaa cgactctttc tgattttttt cttctcctaa
      661 ataatcaatt tttaccgtag acggcaaacc cgctttggca atcttttctt gaatttcagc
      721 agttacagtt cctgtcggtc taccggtagt agatgctaat atttgcacag aattcaaacg
      781 attataacgc tccagaatgg cttgaccttg tacctcatta attgaggcta cgtctcccaa
      841 acgaactact gcacctgttg cagtttgcaa catcgtattg gctatatccg taacagactg
      901 tttatccgat ttctcataaa cgatattgat aggatattct tctcctttat ctttgaattt
      961 agactgatca tttcctctga atgcaagttg tatagcattg ccgactgcag gaatactcaa
     1021 acccattttt gcaattttat cacggtcgag tgagattttt atttctgttt ttgcagactt
     1081 cgtactgaat tccacataat cagcccccgg cgttttatat actatgtctt ttactttctt
     1141 ggcagccgcc cacatttcat catcattcgg gctcatcacc acgatttgaa tttgcggttg
     1201 gttgttacct gttacgtttg tcggtttaat ggagaaagta atacccggaa tactgtcaat
     1261 acgcggccgg agaatattgc acagctgatc tgtacttata ctgcgtttgt ctttatccgt
     1321 caaacctact gtaatatcag cctgattcgg tgattcaatg gtagttccca tttgcgtacc
     1381 tacggcacct actttagaga atactttttt tacttcggga tattgcagaa taattttttc
     1441 tacatcttgc actacacgat ttgtttgcat taaagtagct tgcggctcta attcaatcat
     1501 caagctaaac tcacctctgt caccattacc ggcaaaagag ctgccgataa agccggatgc
     1561 tcccagaaac cctattaatc caaatagggc gagcaccata ataatgacat atcttttatg
     1621 cgataatgcc catgctaata atttgccata gttattggta agattagtga tgaatttttc
     1681 aaatcccaag ttgattttac cccaaagtga atgttcattc aggtgcacta attctgcata
     1741 acgcgaggct aacaacggcg tgagtgtaaa cgatacaaat aaactcatca aggtggagaa
     1801 aacgacgacc aaagcaaact cacgtaatat attcccaatt aatcctcccg ctaatgataa
     1861 gggtacaaat accactacat ctactaaggt tatagctaat gccgtaaatg cgatttcact
     1921 tctgccttct aatgaggctt tcatccggtc tttgcccatt tccatatgac ggaaaatatt
     1981 ttcgagaata acgatactgt catccaccaa aatacccacg actaaggaga gtgccagtaa
     2041 agtcatgagg ttgagtgaga atcccatgat atacatcaga ccaaaagtgg gaatcatggc
     2101 tgagggtaag gcgaccagta caaaaaatga acttctgaaa ctgtgtaaaa agaatagcat
     2161 aaccaatgca acaataacaa cggcaagtaa taaatcgtgt atcaccgagg tggctgcttc
     2221 cagtgtgtag acagattgat caactgcaat atcatacttg aaacctttgg tgttgtaatc
     2281 ttttactatc gtgtctaatt ttgctttcac caggctgctc acttccacgg catttgcatc
     2341 tgtttgttta gagatttcca tacctaaacc cactctgccg tttatgcggt tgatggaggt
     2401 aggggttgat gtagcatctg taatcatcgc cacatctccc aaggtcaccc ggcttcccat
     2461 tctgttttct cttacaatca ggtttcggat ttcatcaatt gatttgaatt tttcatctaa
     2521 gcgtatggta taacgcaact ccttgtcttc aatatttccc gctggatagc tcatattgct
     2581 gcccgctaag gtttggtaaa tctgggcaga agaaatgctg tatgctttca gtttatcatc
     2641 atttaatttt acttcaattt cacgtttaat accaccaatt aatcgaactt gtccaacgcc
     2701 ctgcaaattg agtaaaagtg gctttacggt aagattgacg aagtcataca attcggcatc
     2761 attcatattt gcaaaagtcg tgaggcgtaa aacgggtatt tcactggagc tgaatttgtt
     2821 gatcaccggc tcatcagctt cttgaggcaa gtcatttttt atctggttta tctttcgttc
     2881 tacatcctgc tgtgccagtg tagtatttgt tttttgtttc aattgaacaa ttaccgtaga
     2941 cacattttcc atagaagaag aagaaacaat atccacgcct tcgatactgg atgctgcatc
     3001 ttcaattttt ttagtaacac tattttgaac ctcttcggaa gatgcacctt tataaatggt
     3061 ttggatagat aatatcggag cttcaaactt aggaagtaag ttgtaattca atttgctgta
     3121 acccattata ccgaataaaa tcagtactaa aaagactaca gaaattaata aaggtcgctt
     3181 tacagctatt tcggttattg acattttact tattgtttat gatttgaact ttactgtttt
     3241 cgttgatatt gattaatccg ctggtgatta ctttttcacc cggtactaaa cctgaacgca
     3301 ctactatgtt ttgcccgttt tcttcaccga gttctaattt tcttacttga acagtattgc
     3361 cagatactac ataaacatat ggatttttag tgccttccac taaggccatt ttcggtattt
     3421 gcagggcttc ggcaggcttt ttgaatgcga attttacact tacataggtg cctgctttat
     3481 agccgctatt gggtatttct acttctacgc gatagttgtg attttcatct ccttttggtg
     3541 ctatatattt aataacgcct ctggttagtt ttcctggaaa tactacagag gatatttgtg
     3601 cggcttgtcc tgtttttaaa tcataaacat tgttttcact tacaaataca acggctttca
     3661 atacagaaac atccatgatt gtggctaatg gaacacccgg attggcaaac tctccggcat
     3721 tggcattttt ctgagtaata attccgctta ttggcgcata aatattatta tctgctattt
     3781 gctgctgcag gttttctttt tgaattttag aggtctctat attgtaatta atgtcattca
     3841 cggcatttgc aggagcagca tcaccggcaa tcaaatcttg tgtacgcttt aaatcatcct
     3901 gcaatttttt aatcgttaca tcggtgcttt taataccaat ttcacgctgt ttcgaatcaa
     3961 tttttccaat aagctgtcct ttgcttacgt gctgccctat ttcaacattg aaagcagcta
     4021 atttgccggg ctgagtggct gtaatgatac cgcttttatt gatatccaat actgcaggaa
     4081 gtgcataatc tgtgctgatg ggcaagtatt gagcgtcaaa agtagataca gaaaccggca
     4141 cgtgtgtgcg gtctgtaggc gtattgtttt tgtcaattac tttcttgttt tgcgtgagct
     4201 tgtaagcaat taaagctgta attgccacta gaataatgcc tgctgtgatt ttacttttca
     4261 tcaatggtta tttgtagata taaatattat tgttagctct gtcaacgtcc agtttagact
     4321 ggatgtattt taccatagcg gtaaaataat tttgttctgc ttctttaaat gcgtactcag
     4381 cccctatcca gtctgtatag gagataaccc ctttctgata ttgtaaggtt gtaacatcca
     4441 gaatattgcg ggccatttca taatttgctt tattgatgtt caaattctcg acggcattat
     4501 ccatttgagc ctgcgcattt atcagctgta attttaatcc ttcttctttt tgttttaaat
     4561 cctctttcag gttggcaata ttgagtttgg attgtcgaag agatgagctg gtacgcaatc
     4621 cgtcaaagat ggggatgtct acactaaatc ccagtgcaga gaaatcgtgc cagttcgtaa
     4681 aagatttgga gaatttagac ccaaaggcat ttgctccata acgggcataa aatgagattt
     4741 gcggcatgta gcctgctgta atgcgttttt gtaaaatgcc ttgaagttgt atgtttttgc
     4801 gcattaattg caattcgatt cgatttctca cgtttgccgt atcagaataa gttttaaggt
     4861 caataacttc tttgttataa actgtatcaa tagcaataat actatcgaga ggcataccca
     4921 ttgcatactt gagattgtta aaagccactg atttgttagc cgctaatatt tcttcttgac
     4981 ttaaaatggt gttcagacta acttgtactt tatcgacatc aacctgacgg atgacacctt
     5041 tttctttttg taatttcaag attgaaatag ttttcgacag ccgttcttca ttttggaaga
     5101 ttaatttctc attttcatta attagcaaaa ttgtataata ggcagctatg gtattataaa
     5161 ccaaatcttg ctgtgttttc tcttttttca tcttggcaat ttcgtagccg ggctgtattg
     5221 cttttattcc ttgaatataa gaccagttga aaatcatctg ctcgagttgc aatgtagctg
     5281 ctgtattgta tttatttcca atctgtaatg tttgttcagg aattgttatt ccgcctaatg
     5341 acatagaggg aaattgtgta gattgtaatt ttaaattatc atcccacttc agttgtccgt
     5401 atacttgagg taaataagca gattgtcctt ctcgttttct ggcatatgct atatctacat
     5461 cattgtttga catgattacc gaggggttgt attttagtgt ataggcaatg cattgttgta
     5521 aattgtactg cgtttgtgcc tttgtattta cagtaaagaa caaggtgatg aataaagcaa
     5581 taagtaaata ttttacgtct ttcattaaag tttcattttt tttatcaatt tttgaaataa
     5641 ctcaatgccg gcaggtgtgg ctatggcata aatattgtat ttaagtactt ccaggtgaat
     5701 atcagtaggt ttgtgctcgc tttgaatggg ctgcaagggc aaaaaactaa gttgtaattg
     5761 aattagcgtg gtaatttcaa tagaaatctc ttttctgaaa taaccttgtt taatcccttt
     5821 tttaatatta cgggcaatgt ttttatgtgc gaaaacatca cgatgatttt ctacgagcgt
     5881 ccagctctcc ggaaagaact tcatcaagtc gttaataata gcaggatgga agatttttaa
     5941 ttcttcctga atatgtaagc cgatttgtaa taattcttca atagcattat ccgtcttttc
     6001 agagatggca tgtatttctc tttcatcttc ttttaaatcc atagctacgc aaaaatgtac
     6061 taagtcggct ttgttgggaa
//
LOCUS       DAOQII010000146         6061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7382_length_6061_cov_4.173660, whole genome shotgun sequence.
ACCESSION   DAOQII010000146 DAOQII010000000
VERSION     DAOQII010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7382_length_6061_cov_4.173660"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /gene="ilvB"
                     /locus_tag="PLF48_07790"
     CDS             <1..1607
                     /gene="ilvB"
                     /locus_tag="PLF48_07790"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNF49262.1"
                     /translation="MPIYDALWHYKDAITHVLTRHEQGATHAAQGWARIRGNAGVVFA
                     TSGPGATNLVTGITDALMDSTPMVCITGQVPRHLLGTDAFQETDIVGITMPVTKWNYQ
                     ITKAAEIPEIIAKAFFIANSGKPGPVLVDITKNAQFELMEWTDYKKCTHLRGYVNLPK
                     LNETAIEQAAQLLNTAKKPLILSGQGVIISQAREILESLSHKTGIPVATTMQGLGSFS
                     PEHDNFVGLPGMHGNYSVNIKMNECDVMFAIGMRFDDRVTGDVTRFAKQAKVIHIDID
                     EIEIGKVVKTDIGIKADAFDALKALLPKLNENNHKDWIASFKVLDKMEMDEVIENAIH
                     PKSGGIKMGEIMNLINEKTQGDAIIIPDVGQHQMYAMRYYQYHQLHSWIASGGLGTMG
                     FALPATIGAQMAAPHRTVVGIIGDGCFQMTIQELGTIKQQNLPVKIVIFNNNYLGMVR
                     QWQQLFFDNRYSQVELKNPDFVAIAKGYGIASAKVEKREDLGLALDVMFAHDGAYVLE
                     VTVEKEENLFPMVPSGAACSDVILKP"
     gene            1760..3250
                     /gene="mqo"
                     /locus_tag="PLF48_07795"
     CDS             1760..3250
                     /gene="mqo"
                     /locus_tag="PLF48_07795"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HNF49263.1"
                     /translation="MDNQEKQQFDIALIGAGIMSATLATLIKELQPDFNIIVYEKLDA
                     VASESSDAWNNAGTGHSGLCELNYTPENELGNIDISKAVKIMEQFEISKEFWAYLVEK
                     NYVEANDFIHSVPHMSCVFGQEDVDFLKKRFTALSQNPLFSQMKYSEDKAELHNWIPL
                     MMEGRSDDEIMAATRVEIGTDVNYGDLTRLLFKHLEKNHDVKVHLNRNVTKLSQQEDL
                     SWNIEVEDENDEGGFTLNAKFVFIGAGGGALPLLEEAEVEEAAGYGGFPVNGQWLICK
                     NRELIEQHHAKVYGQAANGAPPMSVPHLDTRIINGKKELLFGPFAGFSTKFLKHGSFL
                     DLPLSINRENLLPLIQAGLNNIELTEYLIRELLKSFDDKMDELSKFVPLANHTDWEIA
                     VAGQRVQIIKKDKEKGGTIEFGTEIITTDDKTLAALLGASPGASTSVYIMLKILKTCF
                     EPLFGSEAWQAKFKEMIPTYGTDLSDHELLAIKTRAASHAILELDN"
     gene            3641..4426
                     /gene="mazG"
                     /locus_tag="PLF48_07800"
     CDS             3641..4426
                     /gene="mazG"
                     /locus_tag="PLF48_07800"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNF49264.1"
                     /translation="MSAELSSFERLLQIMNELRAQCPWDKKQTIHSLRKLTIEETYEL
                     SEAILNDDWKNIKEELGDLFLHLVFYAKIADEQKQFTVDEMLNAVCDKLIHRHPHIYG
                     DVEVKNEEDVKKNWEQLKLKEGKKSILEGVPNGLPPVTKAQRIQEKVRQVGFDWDYKE
                     QVMDKIEEEIAELKEAVQTGNPDEIELEFGDVLFALINYARFIDVDAEKALDRTNKKF
                     MERFMWMEKYAKEKQLNLHEMNLAQLDEIWNLAKEAVKKTNSN"
     gene            4436..>6061
                     /locus_tag="PLF48_07805"
     CDS             4436..>6061
                     /locus_tag="PLF48_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49265.1"
                     /translation="MTWHFKKYSNFFALHKWKISIAIGLLLLIISIATDTIQTTQPSL
                     YRAKRMLEQKIRHKQEDAEKIVNDTIFLHSKFNYSAVEKQFSSFSENKDLFIYVYQDG
                     ILKYWSNNEFIPENIEKIAESKTNFIKQGNGFYCIVYKEYEKQKVQVFVFIPVYYHFL
                     INNQFLRNGFVFTHPFLERFVISSRENKNIVSVNNMQGNYLFSIRTSDNIRQIYNPYT
                     VFIELLGLLLLFWALYRFTKQLLKENRKSEAILLILCTVVFFEVLFNQLNIFSITKTS
                     QLFASTLFASPYLGDSLGVLLIRSFLVTWCLSFLRYFKSGGKSSYITVIFPILFYLLL
                     IFVIQSIILNSVISFNFYYVNSVNIYTFISLVIFGLCFSGFLFVQKWAGNFQDDKKFV
                     WIEIILTLVIFSIAYIFKLFGSLWYTLFLMLWFISFSLFYTNESSFFKRFSNYSFLSN
                     LILLAFISFLTSIIIIYNTNQKDIDKRKYRMKELIADRDHGEEFNLTEAESEINNDKF
                     IDNYFKNPYIYYVDIEDRITDKYFKRFQKRYSIN"
BASE COUNT         2047 a         1044 c         1138 g         1832 t
ORIGIN      
        1 ttatgccaat ttatgatgca ctttggcatt ataaagatgc tataacgcat gtacttacca
       61 gacatgaaca aggcgcaact catgctgcac agggttgggc tcgcataaga ggaaatgctg
      121 gtgttgtttt tgcaacttca ggtcccggtg ctacaaactt ggttacgggc attaccgatg
      181 ctttaatgga ttcaactcct atggtatgca ttaccggaca agttcccaga catttattgg
      241 gcacagatgc atttcaggaa acagatattg taggtattac gatgccggtt accaaatgga
      301 attatcaaat tacaaaagca gcagaaatcc cagaaattat tgcaaaggcg ttttttatcg
      361 ctaatagtgg caaacccggt cctgttttag tagatattac aaaaaatgca cagtttgagt
      421 tgatggaatg gacagattat aaaaaatgta cgcatctgcg tggttatgtc aatctgccga
      481 aattaaatga gacagcaatt gaacaggctg cacagctact caatacagca aaaaaaccat
      541 tgattctaag tggtcaaggg gtaatcatat ctcaagcaag agaaatactg gaatcgctgt
      601 cacacaaaac cggaataccc gttgcaacta ctatgcaagg tttaggaagc tttagtcctg
      661 agcatgataa tttcgttgga ttgcccggta tgcatggcaa ttattctgta aatataaaga
      721 tgaatgaatg tgatgtcatg tttgccatag gcatgcgctt tgatgacagg gtaactggtg
      781 atgtaacccg ttttgccaaa caagctaaag tcattcacat tgatatcgat gaaatagaaa
      841 ttggcaaagt ggtaaaaact gacataggca taaaagccga tgcatttgac gcccttaaag
      901 ctttattgcc taaactgaat gaaaacaatc ataaagactg gatagcaagc tttaaagtcc
      961 tggataaaat ggaaatggat gaagttattg aaaatgccat tcatcctaaa tccggtggta
     1021 ttaaaatggg cgaaatcatg aacttgatta atgaaaaaac acaaggcgat gctataataa
     1081 tacctgacgt gggacaacat caaatgtatg ctatgcgcta ctaccagtac caccagttgc
     1141 attcctggat tgccagtggt ggattaggta caatggggtt tgctttaccg gcaacgatag
     1201 gtgcacaaat ggcagccccc cacagaacag ttgtaggtat tatcggcgat ggttgtttcc
     1261 agatgactat tcaagaactg ggtactataa aacagcaaaa tttaccggtt aaaatagtca
     1321 tttttaataa taattatctg ggaatggttc gtcaatggca acagctcttt tttgacaatc
     1381 gctattcaca agtagaatta aaaaatccgg actttgtagc cattgctaaa ggctatggta
     1441 ttgcatcagc aaaagtagaa aaacgcgaag atttgggttt ggcattggat gttatgtttg
     1501 cacatgatgg ggcttatgtg ttggaagtaa ctgtagaaaa agaagaaaat ttatttccta
     1561 tggtgccgag cggtgctgcc tgtagcgatg ttattttaaa accatagaga taagaaactt
     1621 tgtatcatga acaatacact aatttcaaat tcttatcact attcacaata aaagtctaat
     1681 gccatttgtt ttgtgcctta tacctaataa attaactatt cacaatactc tattcacttc
     1741 ttataactca taaatataaa tggacaatca ggaaaaacag caatttgata tagccttaat
     1801 cggtgcagga attatgagtg ctacacttgc aactcttata aaagagctac aacccgattt
     1861 taatattatt gtgtacgaaa aattagatgc tgtggccagt gaaagttcag atgcatggaa
     1921 taatgccggc accggacatt caggcttatg tgaactaaac tataccccgg aaaatgaatt
     1981 gggcaatatt gatatttcta aagcagtgaa gattatggag cagtttgaaa tctccaagga
     2041 attctgggcc tatttagtag aaaaaaatta tgtagaagcc aatgatttta ttcactccgt
     2101 acctcatatg agctgtgttt tcggacaaga agatgtcgac tttttgaaaa aaagatttac
     2161 tgctttatct caaaatcctt tgtttagtca aatgaaatat tcggaagata aagctgaatt
     2221 gcataattgg atacctttga tgatggaagg acggagtgat gatgaaatca tggctgcaac
     2281 cagagtagaa attggtacag atgtcaacta cggcgatttg acaaggcttt tgttcaaaca
     2341 tcttgaaaaa aatcatgatg taaaagttca tctcaacaga aacgtaacta aactaagtca
     2401 acaggaagat ttgagctgga atattgaagt ggaagatgaa aatgatgaag gagggtttac
     2461 gctgaatgca aaatttgttt ttataggtgc cggtggtgga gctctgccct tgcttgaaga
     2521 agcagaagta gaagaagcag caggctatgg cggatttccg gtaaatggtc aatggctgat
     2581 ttgtaaaaat cgtgagttga ttgagcaaca tcatgccaaa gtatatggac aagcggcaaa
     2641 cggtgctcct cccatgtctg ttccgcattt agacactcgt attatcaacg gaaaaaaaga
     2701 attattgttc gggccttttg caggcttttc caccaagttt ctcaaacatg gatcatttct
     2761 tgatttaccc ttatcaatca atcgcgaaaa tttattgcct ttgattcaag ccggattaaa
     2821 caacatcgaa cttacggaat atctgattcg tgaattgctg aaatcctttg atgacaagat
     2881 ggatgaactc agtaagtttg taccactggc aaatcatact gattgggaaa ttgccgttgc
     2941 cggacaacga gttcagatta tcaaaaagga taaagaaaaa ggcgggacta tagaattcgg
     3001 cacagaaatc attacgacag atgataaaac tttggcagca ctcttaggag catctcccgg
     3061 agcatctaca tccgtttata tcatgctgaa aattctgaaa acatgctttg agcctctttt
     3121 tggcagcgaa gcatggcagg ctaaatttaa agaaatgatt cctacatacg gtacagattt
     3181 atctgatcat gagttattag caataaaaac cagagcagca tcgcatgcca ttttagaact
     3241 ggataactaa ggaaacatat aaaataaatt tacgattgta gttaattcta agcatatcgg
     3301 atattaattt attttttgta catggtgagg gttatgtatt gtacaaattt caaacattgt
     3361 tcaatgcagt aaatcacaca attcacgtat ttaaatgtaa atttcaacat ttcatgtgcc
     3421 ttgttattta catagtcaag ctctagaata acctacagct gccacaggtg atataaacat
     3481 ggggttgttt aatgtaattt taaatactaa tatatataca aaacacaaca tactaaaatt
     3541 aagtaatagt ttgcataatt caaaactgac tcctactgat gactcctttc aatcaaattc
     3601 ttcagttcaa aaaaatcagt agctttgcaa aaaactgtcc atgtctgccg aactatcttc
     3661 ttttgaacgt cttttgcaaa ttatgaacga actccgagcg caatgcccgt gggataaaaa
     3721 acaaacgata cactccttac gaaaactgac tatcgaagaa acatacgaac tttctgaagc
     3781 aatcctcaat gatgactgga aaaacattaa agaggaactc ggcgatttat ttttgcactt
     3841 agtattttat gcaaaaattg cagacgaaca aaaacagttt acagttgatg aaatgttgaa
     3901 tgcagtttgc gacaaactga ttcatcgtca cccacatatc tacggtgatg tggaagtaaa
     3961 aaatgaagag gatgtcaaaa aaaactggga gcaattgaaa ctcaaagaag gcaaaaaatc
     4021 aatactggaa ggtgtaccca atggcttgcc tcccgttacc aaagctcaac gcatacagga
     4081 gaaagtgcgt caggtaggtt tcgactggga ctacaaagag caggtgatgg ataaaataga
     4141 agaagaaata gctgaactga aagaagctgt acaaaccggc aaccccgatg aaatagaatt
     4201 ggaattcggc gatgtgttgt ttgctctgat caattacgct cgatttattg atgtggatgc
     4261 agaaaaagcc ttagaccgca ccaataaaaa atttatggaa cgctttatgt ggatggaaaa
     4321 gtatgcaaaa gaaaaacagc tcaatttgca tgaaatgaat ttagctcaat tggacgaaat
     4381 ctggaatttg gcaaaagaag cagtaaaaaa aacaaatagc aactagatat taagcatgac
     4441 ttggcatttt aaaaaatatt ctaatttttt tgctttacat aaatggaaaa tcagtattgc
     4501 cataggtttg ctattattaa tcatcagcat tgcaacagac actattcaaa ctacccaacc
     4561 tagtttgtac agagcaaaac gcatgctgga acaaaaaatt cggcataaac aagaggatgc
     4621 tgagaaaata gtgaatgaca cgattttcct acattccaaa ttcaattatt ctgctgttga
     4681 gaagcaattc agttcctttt cagaaaacaa agatttattt atatatgtct atcaggacgg
     4741 catactgaaa tactggtcga acaatgaatt catcccggaa aacattgaaa aaatcgccga
     4801 gtctaaaacc aacttcatta aacaaggcaa tgggttttat tgtatcgtct ataaagaata
     4861 tgaaaaacaa aaagtgcagg tatttgtctt tattcccgtt tattatcatt ttttaatcaa
     4921 taatcagttt ctgcgaaatg gcttcgtatt cacacatcca tttttggaaa gatttgtcat
     4981 ttcaagccgg gaaaacaaga atattgtttc tgtgaataac atgcaaggaa attatttatt
     5041 ctctattcgc acatcagaca atatcagaca aatctacaat ccctataccg tatttattga
     5101 actgctggga ctcttacttt tattttgggc actttatcgg tttacaaaac aattattaaa
     5161 agaaaacaga aaatctgaag ccattttatt gattttatgt acggttgttt tttttgaagt
     5221 cttgttcaat cagcttaata tcttttcaat aaccaaaacg tcccaactct ttgcttctac
     5281 cctctttgcc tcgccttatc tgggagattc tttaggcgtt ttgctaattc gttctttttt
     5341 ggtcacttgg tgtttgtcat ttttgcgata tttcaaatca ggcggcaaat catcttatat
     5401 cactgtgatc tttcccattt tattttattt gctccttatt ttcgtcattc aaagtatcat
     5461 actcaactct gtcattagct ttaattttta ctatgtcaat tcggttaata tctacacatt
     5521 tatcagcttg gtgattttcg ggctctgttt ttccggattc ttatttgttc aaaaatgggc
     5581 aggtaatttt caggacgaca aaaaattcgt ttggattgaa atcatactga cgctggtcat
     5641 tttcagtatt gcttatattt ttaaactatt cggcagctta tggtatacgc tcttcttaat
     5701 gttatggttt atttcatttt ctttatttta tactaacgaa agcagttttt tcaaaagatt
     5761 ttctaactac agtttcttat ccaatttaat acttctggca tttatttcct ttttaacttc
     5821 tatcatcatt atttacaaca ccaaccagaa agatattgac aagcgcaaat accgcatgaa
     5881 agagctgatt gccgacagag atcatggtga agaattcaac ctgacggaag ctgaaagcga
     5941 aattaataat gataaattta ttgataatta ctttaaaaac ccttatatct attatgtaga
     6001 tattgaagac cgcatcaccg ataaatattt caaaagattt caaaaacgat attctataaa
     6061 t
//
LOCUS       DAOQII010000147         6012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7471_length_6012_cov_4.661910, whole genome shotgun sequence.
ACCESSION   DAOQII010000147 DAOQII010000000
VERSION     DAOQII010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7471_length_6012_cov_4.661910"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLF48_07810"
     CDS             <1..309
                     /locus_tag="PLF48_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49266.1"
                     /translation="GITLSYPDNTTIFFGTSGMSFIALLFGIIVYISNHKPAKKMIGG
                     NLTAITLKYILSGLLLIVFIIFFKSKTTYHFFYMLILIFIYSIISYTGAYLTQPEKKK
                     "
     gene            complement(331..1104)
                     /locus_tag="PLF48_07815"
     CDS             complement(331..1104)
                     /locus_tag="PLF48_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF49267.1"
                     /translation="MYTAIIVEDELVFRDMLLEMLPKVSLPIRVVATCATLRQAREAI
                     LTYNPQILFLDVELPDGKSIELLENLDESNQGIFEAIFVTSFDKYAIEAIKKNAVDYI
                     MKPVELTGLEIALKKVVRRMQIYAQLEKTEELEKQIKRLESSSQQDKKIMIGTTEGTS
                     FIRIGDIVKLESDSNYTTIFLENKKKFLASKTLSIFEELLEPYNFFRIHRQWIINLTK
                     ISSVENNNGAYTALLTDGSKIEISRRKKKDFFEKIAVNE"
     gene            1161..3338
                     /locus_tag="PLF48_07820"
     CDS             1161..3338
                     /locus_tag="PLF48_07820"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF49268.1"
                     /translation="MHKIANIEEARAFIYHLTLTESKGMNQEELGELLLTCEKFAHTA
                     NDTALLCRTRIFLCNHFVQINDLQLALETGWSAKNMAYDHQLEEEELQSYSGLMATLS
                     ELGDYATLESLIFEYREKLENKQDFDRLVSSYIVSGIQLDNLKEIDKAIENYEKAFQY
                     LEKCTNKEIEIILLNNYGNILIGQNNKKAENLLKNAYDLHTQHNAKHSYYSLLILDNL
                     GKLYTSQLEYDKGITTFNTAIKISKKINHISYTVNLYINLSRLYLDINSISKAAYYLH
                     LAFNYLNQSKNKSLYSEYYLTLSNLYKKRNRYKQAYEFFVKHYEINAELFNESSNDKI
                     RKLQILQDVKNIEKERDHAKMMANLKHDFLANMSHEIRTPINSVLGISYLLQQDQLTD
                     KQLNYVRRLQFSGENLLHIINDVLDISKIEAGKMEICYEIFDFSAWLQLLYDQFLFRT
                     EAKQLAFHLEEQTVSPFYVSGDSHRLSQIMLNLLSNAVKFTESGCITLSSKTVKEEDN
                     KLWIKLSVQDTGIGISQDRINQIFMPYEQANADIKNKYGGTGLGLAICVKLCELMHGE
                     LTVNSKLNEGTTFSLTLPFGFTSNIKTDSKEPSKDICIKNLNLLVVDDLEENRQLFHD
                     LLLSINPEFTIQFAEHGKLAIDALAHFKADIILMDLEMPVMNGTDAAMQIKKKNPNQN
                     IIGHTASILALGKEEIKDLGFDYMLLKPFKIDELIQILDSLST"
     gene            complement(3389..3910)
                     /locus_tag="PLF48_07825"
     CDS             complement(3389..3910)
                     /locus_tag="PLF48_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49269.1"
                     /translation="MKNNMFKFGMSKILKNFSKGLMPLQIDTASGGSGKVIAVSEVDK
                     LISEYLTAVTPEQTVKVNYIVQVLGRDKIATDLEVKLSVNNQQYTAVLKTGESAAVFE
                     FDISTRATTLVTVNLSGKDFSGTLYDTFDINGTFSYNNSLYRITNYYCVSDVINPNNV
                     KLFGRGNGWPPTN"
     gene            4180..4854
                     /locus_tag="PLF48_07830"
     CDS             4180..4854
                     /locus_tag="PLF48_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNF49270.1"
                     /translation="MRIRLFFPFIACLYMLTACSSTKKLPTQIIYIGEHTLPCNAGVM
                     QKQCMQIKWTADQKDWQLFYDQIEGFNYEEGYEYELLISKEKIVNPPADASDTKYTLV
                     KEISKKKVAGNDVSLFTTWKLESFDEPGALMPVMDGMGITFTQKDNAFNGNASCNNIM
                     GNYTLSGNNLKFGTIASTRKMCEHMELESKMIKTLEKTTRYKITGCHLYLFEQDVKLL
                     TFSSCK"
     gene            5161..>6012
                     /locus_tag="PLF48_07835"
     CDS             5161..>6012
                     /locus_tag="PLF48_07835"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNF49271.1"
                     /translation="MHNENSKLLFEQAKLLMPGGVNSPVRAFQSVGGTPVFIKKGKGA
                     KIWDEDGNEYIDFCCSWGPLILGHANEKIEHAIIETVKNGTSFGAPTALENELAECII
                     SNHRFIEKIRFVSSGTEAVMSALRLARGITQRNKIIKFEGCYHGHADSLLVKAGSGLA
                     TLGTSSSAVVPASFVNETIVLPLNDETALRAAFENYTDDIAAVIIEPIPANNGLLLQS
                     HKFLLHLREICTEFGALLIFDEVISGFRVGFEGAAGLYHIQPDIITFGKIIGGGMPVG
                     AYAASAEI"
BASE COUNT         2012 a         1025 c         1016 g         1959 t
ORIGIN      
        1 gggattacac tcagttatcc tgataataca acaatctttt ttggaacttc aggcatgtca
       61 tttattgccc tgttgtttgg cataatagtt tatatcagca atcataaacc ggctaaaaaa
      121 atgataggcg gcaatttaac agccattacg cttaagtata tcctgagcgg attattgctc
      181 attgtattta ttattttttt caaatctaaa acgacctatc acttttttta tatgctgatt
      241 ttgattttca tttattcgat aatcagctac acgggtgcct atcttacgca acctgaaaaa
      301 aagaaataag gaaatcagga ttaacatttg ctattcgttc acggctattt tttcaaaaaa
      361 atcttttttc tttcttctcg aaatttctat tttgcttcca tcggtaagta atgccgtata
      421 agcaccgttg ttattttcta cactgcttat ttttgtcaga ttgataatcc actgacggtg
      481 tattctgaaa aaattataag gttctaataa ttcttcaaag atggatagcg ttttggatgc
      541 tagaaatttc ttcttgttct ctaaaaaaat agttgtatag ttgctgtcag attctagttt
      601 gacaatgtcc ccgattctga taaaactggt gccttcagta gtaccaatca taattttttt
      661 gtcttgctgc gagcttgatt ctaaacgctt tatttgcttt tcaagttcct cagttttttc
      721 aagctgggca taaatctgca tacgtcttac cacttttttc aacgcaattt ccaaaccagt
      781 gagctctaca ggtttcatga tataatctac tgcattcttt tttatggctt caatagcata
      841 tttatcaaat gaagtaacaa aaatagcttc gaatatacct tgattgctct catccagatt
      901 ttccagtaac tctatactct taccatccgg taattcgaca tccaaaaaca aaatctgtgg
      961 attgtatgtt aaaatagctt ctctggcttg gcgcaatgta gcacaagttg ccactactcg
     1021 aatgggcaaa ctgactttag gaagcatttc caaaagcata tcccgaaaaa caagttcgtc
     1081 ttcgacgata atagcagtat acatatccaa aacagtattt ggttaaaaat aagaattctt
     1141 ttacatttat caaattaact atgcataaaa tcgctaatat tgaagaagct cgtgcgttta
     1201 tttaccattt aacgctcacc gaaagcaaag gcatgaacca ggaagaactg ggtgaactgc
     1261 tgttgacatg tgaaaaattt gcccatactg ccaacgatac agccttgtta tgccgcactc
     1321 gaattttttt atgcaatcat tttgtgcaga taaatgattt gcaattggct ttagaaacag
     1381 gctggagtgc aaaaaacatg gcgtatgatc accagttgga agaagaagaa ctgcaaagct
     1441 attccggctt gatggctacc ctgagtgagt tgggtgatta tgcaacattg gagtctttaa
     1501 tttttgaata ccgggaaaaa ttagaaaaca aacaggactt tgaccgttta gtgagttcat
     1561 acattgttag tggcattcag cttgataatt tgaaagaaat tgacaaagca attgagaatt
     1621 atgaaaaggc atttcagtac ttggaaaaat gtacgaataa agaaattgaa attatcttac
     1681 tcaacaatta cgggaatatt ttaataggac aaaacaataa aaaagcggaa aatttgctaa
     1741 aaaatgctta tgatttacat acgcaacata atgcaaaaca tagttattat tcactgctca
     1801 tattagataa tttaggaaaa ttatatacta gtcaattaga atatgataag ggaattacaa
     1861 cttttaatac agctatcaaa atatcaaaaa aaataaatca tatatcatac actgtaaatt
     1921 tatacattaa tttatcaaga ttatatttag atataaattc tatttctaaa gctgcctact
     1981 atttacattt ggcatttaat tatttgaatc aatcaaaaaa taaatccctt tactcggagt
     2041 actatttgac actttctaac ctttacaaga aaagaaaccg gtataagcag gcatatgaat
     2101 tttttgtaaa gcattacgaa attaatgccg aactgtttaa tgaatcatcc aatgataaaa
     2161 tcaggaaact gcaaattttg caagatgtaa aaaatattga aaaagaacgc gaccacgcta
     2221 aaatgatggc gaatctgaag catgattttt tagccaatat gagtcatgaa attcgcacgc
     2281 cgatcaacag tgttttaggc atctcctact tattacagca agatcaatta actgataaac
     2341 agttgaatta tgtacgccga ttacaattca gcggtgaaaa cttgttgcat attatcaatg
     2401 atgtgttaga tatttccaaa atagaagccg ggaaaatgga aatttgttat gaaatatttg
     2461 atttttcagc ttggttacaa ttactctatg atcaattttt gttcagaact gaagccaaac
     2521 aacttgcttt ccatttagaa gaacaaacag taagtccatt ttatgtatca ggagatagtc
     2581 atcgtttgtc tcagattatg ctgaatttat tatcgaatgc cgtaaaattt accgaatcgg
     2641 gctgtattac cctttccagt aaaacagtaa aagaagaaga taataaatta tggattaaat
     2701 taagtgttca ggatacgggt atcggcatat cacaagacag gataaatcaa attttcatgc
     2761 cttatgaaca agcaaatgct gatattaaaa acaaatacgg aggcaccgga ctaggcttgg
     2821 ctatttgtgt aaagctgtgc gaactgatgc acggagaatt gaccgttaac agcaagctca
     2881 acgagggtac tacatttagt ctgaccttgc cgtttggttt tacatcaaac ataaaaacag
     2941 attcaaaaga accaagtaaa gatatttgta taaaaaattt aaatttatta gttgttgatg
     3001 atcttgaaga aaacagacaa ctttttcatg atttattgct tagtattaat cctgaattta
     3061 ctattcagtt tgcagaacat ggtaaattgg ccatcgatgc cttagcccat tttaaagcgg
     3121 atatcatttt aatggatctg gaaatgccgg tgatgaatgg aacagatgct gctatgcaaa
     3181 taaaaaagaa aaacccaaat caaaatatca ttggtcatac tgccagtata ctggcattgg
     3241 gaaaggagga aatcaaagac ttaggttttg attatatgct tttaaaacct tttaaaatag
     3301 atgaactaat ccaaatatta gatagtcttt ctacatagta ttcataataa aaaatgcggt
     3361 caactgaccg cattttattt taaaaaattt agttagttgg aggccaaccg tttcctctgc
     3421 caaacaactt aacattatta ggattaatta catctgacac acaataatag ttagttattc
     3481 tgtataatga attattataa gaaaaagtac cattaatatc aaaagtatca tacagagtac
     3541 ccgaaaaatc ttttccggat aaatttaccg ttaccagagt tgttgcacgt gtcgaaatat
     3601 cgaattcaaa tacagctgca ctttcacctg ttttcaggac agctgtatat tgctgattat
     3661 ttacactcaa tttaacttct aaatctgtgg caattttatc tctgcccagc acttgcacga
     3721 tgtaatttac tttcacggtt tgctcaggcg taactgctgt taagtattcg ctaatgagtt
     3781 tatccacttc tgagacggca atcactttgc cgctaccgcc agaagccgta tctatttgta
     3841 aaggcatcag ccctttgctg aaatttttca gtattttact cataccgaat ttaaacatgt
     3901 tgtttttcat atcgcttttt tattgaatta agaatagctt aaatttagga attatatttg
     3961 attatagtcg gcaacttgcg taaacggtat taattagtga gtaaacggta aatgctgctc
     4021 ttctacctaa caagtttcat aaattaattc tcttaaataa acaaaattct tgttgttgac
     4081 aattatcaaa tcgaaggctg aaaaaaaata aaatttttca gtattgagaa taactgtcta
     4141 tcttgtggct acttattcac atatcctaaa ttagctttga tgcgtatccg attatttttt
     4201 ccatttattg cctgcttgta tatgcttaca gcttgttcat ctacaaaaaa actgccaaca
     4261 caaataattt atattggcga acacactttg ccttgcaatg cgggcgtaat gcaaaaacaa
     4321 tgcatgcaga tcaaatggac tgctgaccaa aaagactggc aattatttta tgaccagatt
     4381 gagggattta attatgaaga aggttatgaa tatgaattgc ttatcagcaa agaaaaaatt
     4441 gtcaacccgc ctgcagatgc atctgatact aaatatactc tagtcaaaga aatcagcaaa
     4501 aagaaagttg caggtaatga tgtatcctta ttcacgactt ggaagttaga aagttttgat
     4561 gaacccggcg cactaatgcc tgtaatggac ggaatgggaa tcacatttac acaaaaagac
     4621 aatgctttta atggaaatgc cagctgtaat aatattatgg gtaattacac tttatctgga
     4681 aacaacttga aattcggtac gattgcatct acacgaaaaa tgtgtgaaca catggaattg
     4741 gaatcaaaaa tgataaaaac gctggaaaaa acaacccgtt ataaaataac aggttgtcat
     4801 ctctatcttt ttgaacaaga cgttaaacta ctcacctttt caagctgcaa gtaagcatta
     4861 aacggtgctg caactgttag gatatttact taagcaattc cacattacat ttaattgaaa
     4921 atttttcatt tgaatttagt aaactatatt tattattagt atattactaa taaaactact
     4981 atattcaagt aacaatgagt attattacta agtaaaagaa ttatctataa attcttattc
     5041 aaattataag ctcttaacac gtttgtctgt atatttaaat ttattctaac gcatgctgat
     5101 aaattcccat ttcacttaag ctcatttttt cctaacttta cagcttattc aagctatttt
     5161 atgcataacg aaaactcaaa attgcttttt gaacaagcta aattattaat gcccggtggt
     5221 gtcaattccc ctgtacgcgc attccagtcg gtgggcggaa ctcctgtatt tataaaaaaa
     5281 ggtaaaggtg ctaaaatttg ggatgaagac ggtaatgaat atatcgattt ttgttgttcc
     5341 tggggaccac tgattttggg tcatgccaat gaaaagattg aacatgccat cattgaaacg
     5401 gtaaaaaacg gtacttcttt cggtgcacct actgctttgg aaaatgaact ggctgagtgc
     5461 atcatttcga atcatcgctt tatcgaaaaa atccggtttg taagttccgg cacagaagcc
     5521 gtgatgagtg ctttgcgttt ggcaagaggc ataacccaac ggaataaaat tatcaaattt
     5581 gaaggatgct atcatggtca tgccgattca cttttagtaa aagccggttc aggtttagct
     5641 acactaggta cttcctcatc agcagtcgta ccggcgtcct ttgtcaacga aactatagtt
     5701 ttgcctctta atgatgaaac ggcattaaga gctgcttttg aaaattatac cgatgatatt
     5761 gccgctgtca ttattgagcc tattcctgcc aataacggct tattgttgca atcacataaa
     5821 ttcctgttgc acttgcggga aatatgtact gaatttggtg cgttattaat ttttgacgaa
     5881 gtcatttcgg gattccgtgt cggttttgaa ggagctgccg gtttatatca tattcagccc
     5941 gacatcatca cctttggtaa aatcatcgga ggcggtatgc ctgttggtgc ttatgctgct
     6001 tccgctgaaa tt
//
LOCUS       DAOQII010000148         6003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7490_length_6003_cov_6.290686, whole genome shotgun sequence.
ACCESSION   DAOQII010000148 DAOQII010000000
VERSION     DAOQII010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7490_length_6003_cov_6.290686"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..912
                     /locus_tag="PLF48_07840"
     CDS             76..912
                     /locus_tag="PLF48_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNF49272.1"
                     /translation="MKIFEKLPVHFIFSELRKRIQGTIFSDKRFIQLITLKPIELYQG
                     ETEVTIDTAVVKLKIDSHVENMMQRRIERTFDWLIMNGAGNSGNTFEYAFDVIIKACA
                     DYKDVSSFFNFHFAHKKYDIEPDKYALELGEFSFGNEATKMSVSLPFTFYARWWKFRR
                     VLSGVAHFRGSLNFHQPKFVIKTRNLNYTLETSNPLLRWIDQMYHAKLISFLHGFLQY
                     NFKSELETAQREAQEQIDKIQYQNSWIYGKIEELDLERITIEPDGLHGVFLAKGKLNL
                     LA"
     gene            986..1366
                     /locus_tag="PLF48_07845"
     CDS             986..1366
                     /locus_tag="PLF48_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNF49273.1"
                     /translation="MAKKIIYTDDAPEPIGPYSQAVYSNGMLFVSGQIPIEPFSGALI
                     KDGIAAETEQVMQNIQAILDEAEMDFSNVVKCSIFLTDMKDFPKVNEIYGKYFAVHPP
                     ARETVEVSCLPKNANVEISCIAVR"
     gene            1376..2056
                     /locus_tag="PLF48_07850"
     CDS             1376..2056
                     /locus_tag="PLF48_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49274.1"
                     /translation="MNLKVLLFTAFILGRYWVNAEPPKVPVANAGLNYNCVTAKDTLK
                     HRQNRGEFTGFYMPVKFTKDKFYYFYITPQNYTRQILSLSDISEEFMYCYNKANQKYM
                     DSVYRIDFFRKSDSVLAAYDDQGKGYRNAEFPGGAPALQKYMKKIDLPKDAKPEDTSS
                     AIKKIRVYYAFDVNTDGKISNIKLAKSNCKVCEQPVLDAILKMPDFIPATEAGKLKKV
                     KYILPFIK"
     gene            2130..3137
                     /locus_tag="PLF48_07855"
     CDS             2130..3137
                     /locus_tag="PLF48_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNF49275.1"
                     /translation="MNFNDRFRQVVFFIVLAGLAILIFIQAKSTIPGILSAITLYLLN
                     RVWFLKLIHRHKWNKTLTVIFFITIDTLVLFIPFLLTSVFIFPKLVYLTNNANELFQG
                     VQKLLEQLQNRFDIQIATKENLMALPKYISGVLPNVFSSAAGMITNLGIMYFTLYYML
                     QNAEQMEKKLTEFLPLRKENTKQLAKETKDIVISYSIGIPVLALAQGICATIGYAVFG
                     ISDPLLLGFLTGLCSVIPFVGSALVWVPLVVYLFAVGPQSAAIGLLAYCLIVVLNVDN
                     ILRMVLLKAFADIHPLITLFGVIVGLPLFGFLGLIFGPLLISYLLLLMKIYVNEFSNQ
                     N"
     gene            3147..4067
                     /locus_tag="PLF48_07860"
     CDS             3147..4067
                     /locus_tag="PLF48_07860"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNF49276.1"
                     /translation="MQVKENIELAKQSVLGVSIGDAFGDSFFGAIENIDEYIYRRKIP
                     CTRWEFTDDTVMSIAILEELETDGHINQDRLVKRFIKNLELDPNRGYGATLRMIMREI
                     ADGKMWQDVSPTVFDGQGSMGNGAAMRVCPLGAYYYNDLERVKNEAILASEITHSNTE
                     AINGAIAVAISTALATRNRINKQSMHPNEFLDSVCVFLDDTDTKSKIEKAKHIPISYH
                     IQTIKHILGNGTNMTAQDTVPFALWCVANYMQNFEEALWKAISILGDRDTICAIVGGI
                     TIMNANPSTIPNLWTESVEDYNYSVFRNNN"
     gene            4089..4397
                     /locus_tag="PLF48_07865"
     CDS             4089..4397
                     /locus_tag="PLF48_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49277.1"
                     /translation="MKPFSKSENQNIANHIFSGSTTMIGVSITIISFFKILKVNKETI
                     IDEFLGINTLFFIVSCLLSYITLRNPNMEKTERYADIFFFLGLSGIFIIGLVLLFLEI
                     "
     gene            complement(4401..5159)
                     /locus_tag="PLF48_07870"
     CDS             complement(4401..5159)
                     /locus_tag="PLF48_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNF49278.1"
                     /translation="MVSIETKEKLEYFYVKYNQRSFIENDPISIPHRFTKKQDIEISG
                     LFAATLAWGNRKSIINSSNKLLNLMDNAPYDFIRHHQPKDLIPFQSFVHRTFNTTDVL
                     YFISFLKNYYTQHQSLETIFVADGIHSGLIAFKREFISGEHFPIRTKKHVSSPETNST
                     CKRLNMYLRWMVRSDNNGVDFGIWKTIQPSQLYMPLDVHVENYARKLGLITRKQRDWK
                     TVEELTATLRNIDKDDPVRFDYALFGMGIEKVVL"
     gene            5253..>6003
                     /locus_tag="PLF48_07875"
     CDS             5253..>6003
                     /locus_tag="PLF48_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF49279.1"
                     /translation="MKTFKDKVVVITGAGSGIGRELAKALSKEGALLALSDINEEGLK
                     ETAALCKDQAVFTHVFDVSNKTAYQQFAQKVLQHYKRVDVVFNNAGVALGKAKITDIS
                     YEEFEWLMNINFWGVVYGIKEFLPSLMEQKVAAIINISSLFGLAGIAEQTPYCSSKFA
                     VRGLSEALRMELIGTPVKVYTVHPGGIKTNIANNARITEKDDAKKAKDVLDKFNQTAL
                     RHSADKAAKIIIDGVKNGEEKIMIGAETYMAD"
BASE COUNT         1915 a         1019 c         1197 g         1872 t
ORIGIN      
        1 tttttcgcgt gattttatta tttgattaca acggcataca aaaaatatta aaagtacgca
       61 gtatttttat gtcggatgaa gatattcgaa aaactgcccg ttcattttat attttccgaa
      121 ctcaggaagc ggatacaggg tactatattt tcggataaac gtttcataca gctcattacc
      181 ttaaagccga tagaacttta tcagggcgaa acggaagtaa ctattgacac ggcagttgtt
      241 aagttaaaga tagacagcca cgtagaaaat atgatgcagc gaagaataga acgcaccttt
      301 gattggctaa ttatgaatgg cgcaggcaac agcggaaata catttgaata tgcttttgat
      361 gttatcatta aagcatgtgc cgactataaa gatgtttctt cctttttcaa ttttcatttt
      421 gcccacaaaa aatatgatat agaaccggat aagtatgccc tggagctggg tgagttcagt
      481 ttcggaaatg aggccactaa aatgtcggtt tcattaccgt tcacctttta tgcccgttgg
      541 tggaaattcc gcagagtact cagcggagtt gcacatttca gaggctcatt aaattttcat
      601 caacccaagt ttgtgattaa gaccagaaat cttaattata ctttagaaac gagtaatccc
      661 ttattgcgat ggatagatca gatgtatcat gctaaattaa tcagctttct gcatggtttc
      721 ttacagtata atttcaaatc agaactggag actgcacaaa gggaagcaca agagcagatt
      781 gacaaaatcc aatatcagaa ttcctggatc tacggcaaaa ttgaagaact ggatttggag
      841 cgcatcacca ttgagcctga cgggttgcat ggcgtttttc tggcaaaagg taagttgaat
      901 ttactggcat aagtacacag gtattagctg ttaacatccg tttacatttt ggaattgatt
      961 tttttcttat ttttgaactc aactcatggc aaagaagatt atttacaccg atgatgcacc
     1021 cgagcctatc ggtccttatt cgcaggcagt atacagcaat ggtatgttgt ttgtatccgg
     1081 tcaaatccca attgagcctt tttcaggtgc tttgataaaa gacggtatcg ccgcagaaac
     1141 cgaacaggtg atgcaaaata tccaagctat actggacgaa gcagaaatgg atttttccaa
     1201 tgtggtgaaa tgcagcatat tcctgacgga tatgaaagat tttccaaaag taaatgaaat
     1261 atatggcaag tattttgcag tacatcctcc ggcaagggaa acggtagaag tatcttgttt
     1321 gcctaaaaat gccaatgtag aaatatcttg tattgccgta cgataaacta actatatgaa
     1381 tttaaaagtt ttacttttca ccgcatttat attgggtagg tattgggtaa atgctgaacc
     1441 accaaaagtt cctgtagcca atgcaggatt aaattataat tgtgtcacgg caaaagatac
     1501 cttgaaacac agacaaaaca gaggcgagtt taccggtttt tacatgcctg taaagtttac
     1561 caaagacaaa ttttactatt tttatattac tccgcaaaat tataccagac agatactttc
     1621 cctgtcagat atttccgaag agtttatgta ttgctacaat aaagccaatc aaaaatacat
     1681 ggactctgtg taccgtatag attttttcag gaagtcagat tcagtattgg cggcttatga
     1741 tgatcagggt aaaggctatc gaaatgctga attcccgggc ggtgctccag ctttgcaaaa
     1801 atacatgaag aagatagatt tgccaaaaga tgcaaaacct gaggatacgt catctgctat
     1861 aaagaagata cgggtgtatt atgcatttga tgtcaatact gacggcaaga tatcgaatat
     1921 caaattagcc aaatcaaatt gcaaggtatg cgaacaaccg gtattggatg ccatactgaa
     1981 aatgcccgat tttatccctg ctactgaagc cggaaagttg aaaaaggtaa aatatatcct
     2041 cccttttata aaatagaagg cgaataatta ttcggcaaat tcgcaatata ttccgaagat
     2101 ttattacttt tcgataaaca gatttcgata tgaatttcaa tgacagattc agacaggtag
     2161 tattttttat cgtactcgca ggattagcaa tcctgatttt tattcaagca aaatctacta
     2221 ttcccggaat tctcagtgcg attacactgt atttgcttaa tcgggtttgg tttttgaaac
     2281 ttattcaccg acacaaatgg aataaaacct tgacggtaat attctttatc acgatagaca
     2341 cacttgtttt gtttattcca tttttattaa ccagtgtgtt tatttttcca aaactggttt
     2401 atttgaccaa taatgcaaat gaattatttc agggagtaca aaagcttttg gagcagttac
     2461 aaaaccggtt tgatatacaa attgccacca aggaaaatct gatggcatta cccaaataca
     2521 tttcgggagt attgcccaat gtattctcat cagcagcagg catgattact aatttgggga
     2581 ttatgtattt caccctttat tatatgctgc aaaatgcgga acagatggaa aaaaaactga
     2641 cggagttttt gcccttgcga aaagaaaata caaaacaact tgccaaagaa acgaaagata
     2701 ttgtcatatc ttattccatc ggaattcctg tactggcttt agcacaaggt atttgtgcca
     2761 ctatcggtta tgctgttttt ggcatctctg atcctttgtt gttgggcttt cttaccggat
     2821 tgtgttcggt tatcccattc gtaggctcgg ccttggtgtg ggtgcctttg gttgtctatt
     2881 tgtttgctgt tggtccgcag agtgcagcca tagggttgct ggcgtactgt cttattgtgg
     2941 tactcaatgt agataatatt ttgcgtatgg tgttactgaa ggcttttgct gacatacatc
     3001 cgttaatcac attatttggc gtaattgttg gtttgccctt attcggattt ttaggtctga
     3061 tattcggtcc tttattgatt tcttatctcc tcttgctaat gaaaatatac gtaaacgagt
     3121 tttctaatca gaattaaaaa tcacgcatgc aagtaaaaga aaatatcgaa ttagctaaac
     3181 agtctgtact aggtgtttcg ataggagatg cattcggcga ttcttttttt ggggcaatag
     3241 aaaatataga tgagtatata tatcggcgta agattccgtg tacaagatgg gagtttaccg
     3301 atgatacggt tatgtccatt gcaatattag aagagctgga aacagatggt catattaatc
     3361 aagacagact tgttaaaaga tttataaaga atttagaatt agacccgaac cgaggatatg
     3421 gagctactct gagaatgatt atgagagaaa ttgcagatgg aaaaatgtgg caggatgtat
     3481 ctccgactgt ttttgatggg caaggttcca tgggaaatgg tgctgcaatg cgagtatgtc
     3541 cattaggtgc gtattattac aatgaccttg aaagagttaa aaatgaggca atattagcca
     3601 gtgagattac acattccaac acagaggcaa ttaacggagc aattgctgta gctatttcaa
     3661 cagccttagc tactagaaat aggattaata aacagtcaat gcatccaaat gaattccttg
     3721 acagtgtttg cgtttttctt gacgatactg acacaaaatc taaaattgaa aaggccaaac
     3781 atattccaat atcatatcac atacaaacaa ttaagcatat tcttggcaat ggaacaaata
     3841 tgacggctca agatactgtt ccatttgcat tgtggtgtgt tgccaactat atgcaaaact
     3901 ttgaagaggc tttatggaag gcaataagca tcttaggaga cagagataca atttgtgcaa
     3961 ttgttggagg tattactatt atgaatgcca atccaagcac aattccaaac ttatggacag
     4021 aatccgttga agattataat tatagtgttt ttagaaataa taattaataa agagattgca
     4081 aaataacgat gaagccattt tctaaatctg agaatcaaaa tattgccaat catatctttt
     4141 caggatcaac aacgatgatt ggcgtttcca ttacaattat ctcgtttttt aagattctga
     4201 aagtaaacaa agaaactata attgatgagt ttcttggaat aaatacactg ttttttatcg
     4261 tttcttgcct gctgtcttac atcaccttgc gaaacccgaa tatggaaaaa acagaacgat
     4321 atgccgatat attcttcttt ttggggctaa gcggtatttt tattatcggt cttgttttgt
     4381 tgtttcttga aatctgatgg ctacaaaaca actttctcta tgcccattcc aaatagtgca
     4441 tagtcaaatc gaacaggatc atctttgtct atatttctca atgttgccgt aagctcttcc
     4501 actgttttcc agtcgcgctg cttgcgagtt attaagccaa gttttcttgc ataattttct
     4561 acatgcacat ccagtggcat ataaagttgt gaaggttgta tagttttcca gataccaaag
     4621 tcaacgccat tattgtcact ccttaccatc cagcgcaaat acatattcag ccgtttgcaa
     4681 gtagagttcg tttccggaga ggaaacatgt ttttttgttc gtataggaaa atgctctcct
     4741 gaaataaatt cgcgcttaaa tgcaattaac cccgaatgaa taccgtctgc aacaaagatg
     4801 gtttccagtg actgatgttg tgtatagtaa tttttcagaa atgaaataaa atacaaaaca
     4861 tcagttgtat tgaaggtgcg gtgtacaaag gactgaaacg gaataaggtc tttgggctga
     4921 tgatgacgga taaaatcgta aggtgcatta tccatcaaat ttagcaactt gttactgctg
     4981 ttgataatag atttccggtt tccccaagcc agagttgccg caaatagtcc tgaaatttct
     5041 atgtcctgct ttttagtaaa acggtgcgga atagaaattg gatcgttctc aataaatgaa
     5101 cgttgattgt atttgacgta gaaatactct aatttttctt ttgtttcaat gctaaccatt
     5161 tttacaaatt taaacaagca ttttcttact tattaacttt agataaaccg taatatacgt
     5221 tcttcattgt atttttcagg aaaattaatt tcatgaaaac ttttaaggat aaggtagtgg
     5281 tcatcaccgg tgcgggttcg ggtattggca gagaactggc aaaagcactg agcaaagaag
     5341 gtgcgctgct tgcactatct gacataaatg aagaaggatt gaaagaaaca gcagcactat
     5401 gtaaagacca agcagttttt acacatgttt ttgatgtttc caataaaacg gcttatcagc
     5461 aatttgcgca aaaagtactg caacactaca aacgtgtgga tgttgtgttt aataatgccg
     5521 gagtagcctt aggcaaagca aaaattacag atatttctta tgaagagttt gaatggttga
     5581 tgaatattaa tttttgggga gtggtttacg gtatcaaaga gtttttgcca agcttaatgg
     5641 aacaaaaagt agccgctatt attaatattt caagtttgtt tggcttagcg ggtattgcag
     5701 agcagactcc ctattgttca tctaaattcg cagtgcgagg attatcagaa gcattgcgca
     5761 tggaactgat tggcacaccc gtaaaagtct atacagtgca tccgggtggt attaaaacca
     5821 atattgccaa taatgcacgc attacggaga aagatgatgc aaaaaaagca aaagatgtac
     5881 tggataaatt taatcagact gcacttcggc attcggcaga taaagccgct aaaatcataa
     5941 tagacggagt gaaaaacggt gaagaaaaaa ttatgatagg agctgaaacg tatatggctg
     6001 ata
//
LOCUS       DAOQII010000149         5974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7542_length_5974_cov_4.797094, whole genome shotgun sequence.
ACCESSION   DAOQII010000149 DAOQII010000000
VERSION     DAOQII010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7542_length_5974_cov_4.797094"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLF48_07880"
     CDS             complement(<1..261)
                     /locus_tag="PLF48_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49280.1"
                     /translation="MKKCFVFTFILFFSFTGLIAQNYAVGYKYETLRDVSRNRNIPVL
                     IYYPATATGANQPALHNGNKFPVVSFGHGFLMKGEEFTYLAQK"
     gene            699..1205
                     /locus_tag="PLF48_07885"
     CDS             699..1205
                     /locus_tag="PLF48_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49281.1"
                     /translation="MIKNFFSSIIIAILLIHTQNCIGQNLLSEVPKTKDEFIASEKNV
                     VATIDWLETTPFNQEENKRLKQKALLMVWITDSPTVTLEINAAILTFTKKNPDLLLTY
                     MGGWTKYSLLNNYSTDQIQGNLAGLKSVIRVYKNLSLKKDKEVEKLIELDNKGELENW
                     VNEKLIKK"
     gene            1445..2503
                     /locus_tag="PLF48_07890"
     CDS             1445..2503
                     /locus_tag="PLF48_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF49282.1"
                     /translation="MNHLFRFHTLLIFAGTMFIFSGCKPKTSDSGSTTADNTEVMTTA
                     KAVFQPLPKQADNPENPITPEKVALGKRLYYDAKLSNKGNNTCNSCHNLAEYGADKLP
                     FSVGDDGGNGGRNSPTTLNAALHIAQFWDGRAKDVEEQAGMPIMNPVEMHMGSEADVM
                     NRLQKDETYKKMFAAAFPNEKSALSFMNMKKAIGAFERTLITPSKFDEYLNGNKDALT
                     AEEQAGLNTFIKSGCTTCHSGAAVGGQMFQKFGLFGNYWDLTKSAKVDSGKILITKNA
                     ADLYVFKVPSLRNIAKTAPYFHDGSVSTLYDAIAIMGKAQLNKTLTDDEIKSIAVFLN
                     SLTGTVPADAQVNPFATK"
     gene            complement(2615..3952)
                     /gene="nuoF"
                     /locus_tag="PLF48_07895"
     CDS             complement(2615..3952)
                     /gene="nuoF"
                     /locus_tag="PLF48_07895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNF49283.1"
                     /translation="MSKKLLLQYDHIPGIQTLEVYRQNNGYASLKKAFSMKQEDIVEE
                     VKKSGLRGRGGAGFPTGMKWSFLAKPEGVPRHLVCNADESEPGTFKDRYLMERIPHLL
                     IEGMIVSSFALGANTSYIYVRGEYYYIIKILEKAIDECYKAGLLGKNIMGSGFDLDLY
                     VQPGGGAYICGEETALLESLEGKRGNPRLKPPFPAVAGVWGRPTVVNNVETIAAVVPI
                     INIGGEDYAKIGIGKSTGTKLISASGHLNKPGVYEIELGLPVEEFIYSEVYCGGIRNG
                     KKLKAVVAGGSSVPILPAELILKTANGEPRLMSYESLADGGFATGTMLGSGGFIAMDE
                     DTSVVKNLFTFARFYHHESCGQCSPCREGTGWMEKILKKFISGKAQMSDIDLLWDIQS
                     KIEGKTICPLGDAAAWPVASAIRHFRHEFEEYVRDPQSIKNVKHYYRLNNLAV"
     gene            complement(3986..4510)
                     /locus_tag="PLF48_07900"
     CDS             complement(3986..4510)
                     /locus_tag="PLF48_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNF49284.1"
                     /translation="MTDTATEIKFSEAAQKRADEIIKRYPEGKQKSALLPLLHLAQAE
                     FDGWLSVPVMDYVAGMLQLKPIEVYEVASFYTMFNLKPVGNYLIEICQTSSCWMCGSE
                     DITAYLQKKLDVGIDEPTADGMFHIKLVECLGSCGTAPMFQIGDKFYENLTLEKIDTI
                     LDDLRKENKRSRYC"
     gene            complement(4701..5912)
                     /locus_tag="PLF48_07905"
     CDS             complement(4701..5912)
                     /locus_tag="PLF48_07905"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNF49285.1"
                     /translation="MKSIDAINFKNERALIRVDFNVPFDKQFNITDDTRIRAALPTIN
                     KVLNDGGSVVLMSHLGRPLKKLKEDGSLDVDKFTLRHIVKHLSELLQRDVKFADDCVS
                     DASLEMSTNLKNGDVLLLENTRFHKEEEKGEEQFSKTLAKHGTVYINDAFGTAHRAHG
                     STTIVAHSFPADKKYLGYLMSAEVANADKVLHKAEKPFTAIIGGAKVSDKILIIENLL
                     NIADTIIIGGGMAYTFFKAQGGEIGNSLVEADKIDVALEVLEKAKAKGVQLLLPLDSV
                     IADKFADDAQTQSAESNAIPADCMALDIGEKAIAQFSDVIKHSKTILWNGPMGVFEME
                     KFQNGTKQIALAIAEATASGAFSLIGGGDSVAAINKFNLQEKVSYVSTGGGALLEFFE
                     GKTLPGVAAIL"
BASE COUNT         1909 a         1161 c         1023 g         1881 t
ORIGIN      
        1 tttttgtgct aaataggtaa actcctctcc tttcattaaa aagccatgcc cgaaagacac
       61 gaccggaaac ttgtttccgt tatgtagtgc cggttggttc gctccagtag cagttgcagg
      121 ataataaatc agcaccggaa tatttctgtt tctggaaaca tcacgtaatg tttcatattt
      181 ataacccacc gcataatttt gggcaatcag accagtaaaa gaaaagaata gaataaatgt
      241 aaagacaaaa cattttttca tacacggaat ttttacaaaa ttaaaacaag ttgattatat
      301 tccccaaata aaaatgatat tagaagactg aaatttttat gtgagatata gaaaagttca
      361 tatagttagt atgctaatat aaacacattc ataaaattca acttcattat caattctcta
      421 gtatacgtga atcgacaaat ggatagcata aattcattca tttgttaagt ttttaatagc
      481 aaataactgc aatatgtttc gacatttgta tcaagaaaac tggaatttta gtagaaaatc
      541 taaataatac atgctatttc tgatatatag tcattgtcat ttaggaaata aatatttatt
      601 tttaggtgaa aaattaaaga ttataaggga cacacgactt atactttagg ctttttgaat
      661 ttttatattt atacacgtaa accaaaataa gaactattat gattaaaaat tttttttcaa
      721 gtattatcat tgctattctt ttgattcata ctcaaaattg cattggtcaa aatttattaa
      781 gtgaagttcc caaaactaag gatgagttta ttgcaagtga gaaaaatgta gttgcaacga
      841 ttgattggtt ggaaactaca ccatttaatc aagaagagaa caaaagactc aagcagaaag
      901 cactgttaat ggtttggata acagattcac caactgttac tttagagata aatgctgcca
      961 tcttaacttt tacaaaaaag aatccggatt tattattgac ttatatggga ggctggacga
     1021 aatatagtct tcttaataat tattcaacag atcaaattca aggcaattta gcaggtctga
     1081 aaagcgtcat tagggtttat aaaaatttat ccttaaaaaa agataaagaa gttgaaaaat
     1141 taattgaact tgataataaa ggagaattag aaaattgggt aaatgaaaaa ctaataaaaa
     1201 aataagcagt cctttaatta tctctctata aattaatcag actatattat tttatatatt
     1261 attatttatt ataatacaat acccaacaca gtatacagtt tactttttaa atatattttc
     1321 ataaaaatca aatatttaaa ctgatagcta tactttcaaa tcgcttgatt tacatcaatg
     1381 acttttatca tggattatat ttaatttgtg ttttaaattt attgctaaac aaattgctcc
     1441 ttttatgaac cacttatttc gatttcacac acttcttatt tttgccggta caatgtttat
     1501 attttcaggt tgcaagccta aaacatctga ttccggcagt acgacagctg ataatactga
     1561 ggtaatgact acagcaaagg cagtttttca gcccctgccc aaacaagcag ataatccaga
     1621 aaacccgatt acaccagaga aagtagcatt gggtaagagg ttatattatg atgcaaaact
     1681 ttccaataag ggaaataata cctgcaacag ttgtcataat ttagctgaat atggtgcaga
     1741 caaattacca ttttctgtag gtgatgatgg tggaaacggt ggcagaaact ctccgactac
     1801 tctaaatgct gctttacata ttgctcagtt ttgggatgga agagcaaaag atgtagaaga
     1861 acaagccggt atgccgatta tgaatcctgt ggaaatgcat atgggttctg aagctgatgt
     1921 catgaacaga ttacaaaaag atgagactta taaaaagatg tttgcggcag catttccgaa
     1981 tgaaaaaagt gccctttctt ttatgaatat gaaaaaggct atcggtgcat ttgaacgtac
     2041 tttgattacg ccttcgaaat ttgatgagta tctgaatggc aataaagacg cacttactgc
     2101 tgaagaacag gcgggtctta atacatttat taaatcaggc tgtactactt gtcactccgg
     2161 tgcagcagta ggtggtcaga tgttccaaaa atttggctta tttggcaatt actgggattt
     2221 gactaaaagt gcaaaagtgg attctggtaa aatccttatc accaaaaatg cggctgattt
     2281 atatgtattt aaagtgcctt cactcagaaa tattgctaaa accgcaccct attttcatga
     2341 tggcagcgtg agcaccctat atgatgcaat tgctattatg ggtaaagcac aattaaacaa
     2401 aacgctgact gatgatgaaa ttaaaagcat agcagtattc ctgaattcac ttaccggaac
     2461 tgtacctgcg gatgcacaag ttaatccgtt tgctacaaaa taaattaact tacaaaaata
     2521 cataataagc cgggctagta agtccggctt attttattta tagaaaacca atcttaataa
     2581 agggggttac aactatcaaa gcaatagtag ttgcttatac tgccagatta tttaagcggt
     2641 agtagtgttt tacatttttg attgattgtg gatcacggac gtattcttca aattcgtggc
     2701 ggaaatggcg tatggctgat gctacaggcc aagcggcagc atctcccagc ggacatatcg
     2761 ttttgccttc aattttggat tgtatatccc acaacaaatc aatatcactc atctgtgctt
     2821 ttccgctgat aaatttcttc agaatttttt ccatccagcc cgtaccttct cggcatggcg
     2881 aacattgccc acagctttcg tggtgataaa aacgggcgaa agtaaacagg tttttcacta
     2941 cggaagtatc ttcatccata gcaataaatc cgccggaacc caacatagta ccggtggcaa
     3001 aaccaccatc agccaacgat tcgtagctca tcaatcgagg ttcaccatta gccgttttca
     3061 agataagttc agccggcaaa atcggaacgg atgaaccgcc cgctactact gctttcaatt
     3121 tcttaccatt tctgattccg ccgcaataga cttcgctata gataaattct tccaccggaa
     3181 gtcccagttc aatttcgtat acgcccggtt tgttcaaatg accggatgca gaaatcaatt
     3241 ttgtacctgt tgattttccg atgccgattt tggcataatc ttctccccca atatttataa
     3301 tcggcactac agccgctatc gtctccacat tatttactac agtgggtctg ccccatacac
     3361 ctgccacagc cggaaacggt ggtttcaaac gcggattgcc ccttttacct tcaagtgatt
     3421 ctaacaatgc tgtttcttcg ccacagatat atgcaccgcc accgggctgt acgtacaaat
     3481 ccaaatcaaa accggagccc atgatatttt tgcccaataa tccggcttta taacattcat
     3541 caattgcttt ttccaagatt ttgatgatgt aataatattc gcctctgaca tatatatacg
     3601 aagtgtttgc tcccagtgcg aaactggaaa cgatcatgcc ttcaatcaat aaatgcggaa
     3661 tgcgttccat caaatagcga tctttgaatg tgccgggttc tgactcatct gcattacaaa
     3721 ccaaatggcg aggcacacct tcaggttttg ccaaaaaact ccatttcatg ccggtaggaa
     3781 aacctgctcc gccgcgtcct cgcaatccag attttttcac ttcctccacg atatcttctt
     3841 gtttcatgga aaatgctttt ttcaaagatg cgtatccgtt attttgacga tagacttcga
     3901 gtgtttgtat accgggaata tggtcgtatt gtaataataa ttttttgctc atgggaataa
     3961 aagtttactg atgtaacatc ttcacttaac aatatctgct tcgtttgttt tcctttctca
     4021 aatcatctaa aattgtatct attttttcta gggtgagatt ttcataaaat ttgtcaccga
     4081 tctgaaacat tggtgcagtg ccgcaagagc ccagacattc caccagtttg atatggaaca
     4141 tgccatcggc agttggctcg tcgataccta catccagttt tttctgtaaa taagccgtta
     4201 tatcttccga accgcacatc cagcaactgc tggtctggca gatttctatc agatagttgc
     4261 caacgggttt aagattaaac atagtgtaaa atgaagcgac ttcatacact tctatcggct
     4321 tgagttgcaa cattccggca acataatcca ttaccggcac actgagccag ccgtcgaatt
     4381 ctgcttgagc caaatgcaac aagggtaata gagctgattt ttgtttccct tcgggatatc
     4441 ttttgatgat ttcgtctgca cgcttttgag cggcttcgct gaatttgatt tcagtagctg
     4501 tatcggtcat gtagttgact attgagtaca caaagatacg ctccaatcgt ttattttaaa
     4561 aataaaaagc aggaaaaggt aaataatgaa cgaagttgtt tataaacgat atctacgaca
     4621 tattctagtg agtaattagt aaatagtatt cgcaaatttc tgtaaatata gcatacttaa
     4681 tgtatttgaa tatagaacca ctaaaggata gcagctacgc cgggtaaggt ttttccttca
     4741 aaaaattcca acaacgcccc acctcccgta ctcacatagc tgactttttc ctgcaaatta
     4801 aatttattga ttgccgccac actatcgcca ccgccaatca aactaaacgc acctgaagca
     4861 gttgcttctg caatggctaa agcgatttgt tttgttccat tttgaaattt ttccatttca
     4921 aaaacaccca tcggtccgtt ccacaagatg gttttagaat gtttaatgac atcagaaaat
     4981 tgagctatag ctttttcgcc aatgtccaag gccatacaat ccgccggaat tgcattgctt
     5041 tcagctgatt gcgtttgggc atcatctgca aatttatcag caataacaga atctaaaggc
     5101 aataataatt gcacgccttt agcttttgct ttttcaagca cttccaaagc cacatctatt
     5161 ttatcagctt ctaccagaga attgccgatt tcgccgcctt gggctttaaa aaaggtatat
     5221 gccatgccgc cgccaattat aatcgtatct gcgatattca gcaaattttc aataatcaaa
     5281 attttgtccg acactttagc tccgcctata atggcagtaa acggcttttc tgctttgtgc
     5341 aatactttgt cggcatttgc tacttcggca gacattaaat agcccaagta ttttttatct
     5401 gccggaaatg aatgtgccac aattgtggtg ctgccatgtg cacgatgtgc agtgccaaaa
     5461 gcatcattaa tatatacagt gccatgcttt gccaatgttt tggaaaactg ctcttcacct
     5521 ttttcttctt ctttatgaaa acgggtattt tctaaaagca atacgtcgcc gtttttcaaa
     5581 tttgtagaca tctcaagact agcatcactt acacaatcat ctgcaaattt tacatctcgc
     5641 tgtaataatt cagataagtg cttaacaata tgacgaagtg taaatttatc gacatccaaa
     5701 gagccgtctt cttttagttt tttcagcgga cgacctaaat gtgacatcag caccacacta
     5761 ccaccatcat tcaatacttt attaatggta ggcaaagcgg cacgaatacg agtatcatca
     5821 gtaatattaa attgtttatc aaaaggcaca ttaaaatcca ctcggataag tgctctttca
     5881 tttttaaaat taatggcatc aatagatttc ataaataata atatttttaa taaattcttt
     5941 atctgtgcaa aaataatcta gacgccagcc aata
//
LOCUS       DAOQII010000150         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7562_length_5963_cov_7.314658, whole genome shotgun sequence.
ACCESSION   DAOQII010000150 DAOQII010000000
VERSION     DAOQII010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7562_length_5963_cov_7.314658"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLF48_07910"
     CDS             <1..764
                     /locus_tag="PLF48_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF49286.1"
                     /translation="DYCPISDVQIGRFKSDITIIDSISHNTDLFTEVDEIIAFIKKHL
                     MVEYIITGEPQRTERFDYPLDAIREVVINMIVHRDYRDSSGSVIKIFDNRIEFYNPGK
                     LFGGITIQDLLTGNYTSKSRNKLIAKAFKEAGLIERYGSGILRIQNICKDYGVVAPVI
                     EEAMNGFRVILFNERIKTEEDVGVNVGVNVGVNVGVNDLLDLVRNNPGMNAKWLYQHF
                     DVTNRTVERWIKQLREENKIEFRGAAKTGGYYVTK"
     gene            867..1289
                     /locus_tag="PLF48_07915"
     CDS             867..1289
                     /locus_tag="PLF48_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNF49287.1"
                     /translation="MFTFVCQAIDTILKEIYLTGDIEKYGSGFMRIRKAIKDYPTMKV
                     EYEEIGKGFMATISYTEQKTSSAKRVTENVTENVTERSARILSLMRETPTITTEQIAE
                     ILSVTKRTIIRDIEKMKKNNQIMYIGSAKSGSWKINLE"
     gene            1291..1992
                     /locus_tag="PLF48_07920"
     CDS             1291..1992
                     /locus_tag="PLF48_07920"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNF49288.1"
                     /translation="MTAAIMQPYFMPYIGYFQMVYAVDAFVFYDDVNYIKQGWINRNR
                     ILINGESRFITVPLIQAGSFKMIKDTEIDYRRNELVKLLKAIELAYKKAPFFEEIFPL
                     TAQILEKEYINIAELAKESIVRVSDYLSITTRFYTSSTDFPESRGLDKAERLQAICKK
                     INAEKYINAIGGQELYSKEEFFKENIQLQFIKSLPIAYKQFGNEFVPWLSILDVLMFN
                     SKEEVKKMLTQFELV"
     gene            2005..2586
                     /locus_tag="PLF48_07925"
     CDS             2005..2586
                     /locus_tag="PLF48_07925"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNF49289.1"
                     /translation="MNFKELISQLHEVNHRLRENAIRAVNTSLTIRNWFFGFYIVEFE
                     QNGSDKAGYGEKLLFNIAQELKNIHIPNTDERELRRFRQFYLSYRSAFHYIITQHSIR
                     GLLPPESAKLEKDGNRELSTPELEITDSYYEMHKGDNPPIGILLCTEKDAEHVAFATA
                     GIEDKIFVSKYLVELPDKKELEQFIISSLKKGK"
     gene            2692..3345
                     /locus_tag="PLF48_07930"
     CDS             2692..3345
                     /locus_tag="PLF48_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNF49290.1"
                     /translation="MRIAFYVSGRAGRIRKLLELNRESVFQSTVFVLCDNLQNKDLQA
                     LLKGKNCEYIEFDYNILADTNKARNIVLSDIILDKLTLNNIDYLFCFGEHILKGNLLQ
                     KYHNKIINFHPAILPLYPGLLAIDQARNANAFLYGNTAHFIDSGIDTGLIIMQNVVHR
                     DFFEKYGYDGILDQQLNMLEQIVKWLEADRIQVKENVVTIKDLKANDSVFFPQIEKE"
     gene            3342..4313
                     /locus_tag="PLF48_07935"
     CDS             3342..4313
                     /locus_tag="PLF48_07935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF49291.1"
                     /translation="MKQALVSISCITYNHQDFIEECLNGFFIQQCNFDVELVISDDKS
                     TDGTVKVIERCLQRKPDNFTIRFTAHETNMGAAPNFVWCMEQCAGKYIALCEGDDYWT
                     DPHKLQKQMDFLEANEGYSLVCGGYMSVNTVTGEKNVVLKESPDDSMMTDAGFDITIE
                     MFFQDWYTKTLTTVFRTELLDLGELANYRYTSDTQVFYHLLKKGNGYYIRDVLGAYNM
                     HGSGMHSMVSNEKRSLDGFRVANELFAQNRNDTLVRSYYFSSGLALYSIMPKIKYYEF
                     NKIELLFDLIVQIKNFEEIKRLLIAASGKPGRKLIDWLKIKFNLGKK"
     gene            4605..5222
                     /gene="hisH"
                     /locus_tag="PLF48_07940"
     CDS             4605..5222
                     /gene="hisH"
                     /locus_tag="PLF48_07940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNF49292.1"
                     /translation="MLTLIDYGTGNLLSIQNMFRRIGVPVKISGSAEDIAQADKLILP
                     GVGHFKYGMQQLQKSGLIDVLHERVCLQKVPILGICLGVQLFTQSSEEGDTPGLGWIE
                     GNTVAFNRNSLGKNLKIPHMGWSEIHGYEKSALFTGMPHEPRFYFVHSYHLQLKSESD
                     ALAYTDYGYKFAAGIEHDNILGVQFHPEKSHKFGMKLLENFVKYY"
     gene            5230..>5963
                     /locus_tag="PLF48_07945"
     CDS             5230..>5963
                     /locus_tag="PLF48_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HNF49293.1"
                     /translation="MAAIRVIPSLLIHKDGLVKSVRFKDYKYVGDPINAVKIFNDKEV
                     DELVMMDIDATKENRSPNITRIAEIAGEAFMPVAYGGGITTVQQIKDILFNGIEKVII
                     NKAAHTQPELITEAAALFGSQTIVVSIDVKKNLFGRYKVYTDNGRNNTGIDPAVFAIQ
                     VAQLGAGEILLNSIDRDGTYQGYDLPLIKSVSDAVSIPVIAIGGASSVDDFQQAINNG
                     ASATAAGSMFVFQRPHQAVLISYPDY"
BASE COUNT         2038 a          987 c         1270 g         1668 t
ORIGIN      
        1 cggactattg cccaataagc gatgtgcaga tagggcggtt taaaagtgat ataaccatta
       61 ttgattccat atcacacaat actgatttgt ttacagaagt ggatgagatt atcgctttta
      121 tcaaaaagca tttaatggtg gagtatatta ttacaggcga accacagcgc accgaacgtt
      181 ttgactatcc attagatgct attcgagaag tggtcatcaa tatgattgta caccgtgatt
      241 atcgggacag ttcgggtagt gtgattaaaa tttttgataa cagaattgag ttttataacc
      301 ccggaaaatt gtttggtggt attacaatcc aagatttact taccggaaac tatacctcaa
      361 agtcaagaaa caaactgatt gctaaagcct ttaaagaagc aggattgata gaacgttacg
      421 gttccggaat tctacgcata caaaatattt gtaaggatta tggcgtggtt gcacctgtta
      481 ttgaagaagc aatgaatggc tttcgggtca tactgtttaa tgaaagaata aagactgaag
      541 aggatgtcgg tgtaaatgtc ggtgtaaatg tcggtgtaaa tgtcggtgta aacgatttat
      601 tagatttagt taggaataat ccgggaatga atgctaaatg gctataccaa cattttgatg
      661 taactaatag aaccgtagaa cgctggatta aacaattacg ggaagaaaat aaaattgagt
      721 ttagaggggc ggcaaaaaca ggcggatatt atgttacaaa ataagtaaac aacaggctaa
      781 ataatgaacg atttgattaa agtgaaacgg ataataaagt gtgggcagtc aaaagtaata
      841 tctgcaagta ttgcaggcag aatattatgt ttacttttgt ttgtcaggca atcgatacaa
      901 ttttaaagga aatttattta accggagata ttgaaaaata cgggagtggc tttatgcgta
      961 taagaaaagc cataaaggat tatccgacta tgaaagttga atacgaagaa atcggcaaag
     1021 ggtttatggc tactatttct tatacggaac aaaaaacgag ctcagcaaag cgtgtcaccg
     1081 aaaatgtcac cgaaaatgtc accgaaagaa gtgctagaat tttgagctta atgagagaaa
     1141 caccaaccat tacaacagaa caaattgctg aaatactaag tgttacaaaa aggacaatta
     1201 ttagagatat tgagaaaatg aaaaaaaata atcaaattat gtacattggt tccgcaaaat
     1261 ctggtagttg gaaaataaat ttggaataaa atgacagcag ccatcatgca gccctatttt
     1321 atgccgtata tcggctactt ccagatggtg tatgctgtgg atgcctttgt gttttatgat
     1381 gatgtcaatt atataaaaca agggtggatc aaccgaaacc ggattttgat aaatggcgag
     1441 tctagattta ttaccgttcc attgatccag gcaggatctt tcaaaatgat taaggataca
     1501 gaaatagact atcgccggaa tgaactggta aagttgctca aagccattga gctcgcctat
     1561 aaaaaagcac cgttttttga agaaatattt ccgctgactg cacagattct tgaaaaagaa
     1621 tacattaata ttgcagaact ggcaaaggaa agcatcgtca gagtttcgga ttatttaagc
     1681 attacgacaa gattttacac atcttcaacg gatttccctg aatcaagagg gttggacaag
     1741 gcggagcggt tacaggctat ttgcaagaag atcaatgctg aaaaatacat caatgctatt
     1801 ggagggcaag aactttattc aaaagaagaa tttttcaaag aaaatattca acttcagttt
     1861 attaaatctc tgccgatagc atataagcag tttgggaatg aatttgtccc atggctttct
     1921 atcctggatg tgctgatgtt caatagtaag gaggaagtaa aaaaaatgtt aactcaattt
     1981 gaattagttt agccttctga ttttatgaat ttcaaagagc ttatatctca attacatgaa
     2041 gtcaatcaca gattacgcga aaatgcaatt cgtgcagtaa acacttcatt aactatacga
     2101 aattggtttt ttggattcta catagttgag tttgaacaaa atggcagtga taaagcagga
     2161 tatggggaga aattgctttt taatattgca caagagttaa aaaacataca tatcccgaat
     2221 acagatgaga gagaattaag acgctttcgt cagttttatc tcagttaccg ttctgctttt
     2281 cactacatta ttactcaaca ttcaatccgg ggtttattgc cccccgaatc agctaagttg
     2341 gaaaaagatg ggaatcggga gttatcaacc cccgaattgg aaataacaga ttcgtattat
     2401 gaaatgcata aaggagataa tcctccaatc ggtatattgc tttgtactga aaaggatgcg
     2461 gaacatgttg catttgcaac agccggaata gaagataaaa tctttgtttc taagtatctg
     2521 gttgaactgc cggataaaaa agaattggag caatttatta tcagtagttt gaagaaaggg
     2581 aaataaacga ttcgataacg aatttgtccc gtggctttcc atcctggatg tgctgatgtt
     2641 caatagtaag gaagaagtga aaaaaatgtt gggtaaattt gaattattgt catgagaata
     2701 gctttttatg tctcaggaag agccggaaga ataagaaaat tattagaact caatcgagaa
     2761 tctgtttttc aatcaacagt gtttgtgctt tgtgataatc tgcaaaacaa agatttacaa
     2821 gccttgctta aagggaaaaa ttgcgaatac atagagtttg attataacat tttggcagat
     2881 acaaataaag caagaaacat agttttatct gatattatac ttgacaaatt gactctcaac
     2941 aacattgatt atcttttttg ttttggagaa catattctaa aagggaattt attacaaaaa
     3001 tatcataata aaatcatcaa tttccatccg gcaattttgc ctctttatcc gggcttattg
     3061 gcaattgatc aggcaagaaa tgccaatgct tttttgtatg ggaatactgc acacttcatc
     3121 gattcaggta ttgacactgg cctaatcatt atgcagaatg tagtgcacag agattttttt
     3181 gaaaagtatg gttatgatgg cattttagat caacagttaa atatgcttga gcaaatagtg
     3241 aaatggttgg aggcagacag gattcaggta aaggagaatg ttgtaacaat taaggattta
     3301 aaagcaaatg atagtgtatt ttttccacaa attgaaaaag aatgaagcaa gcattagttt
     3361 ctatttcctg cattacatac aatcatcagg attttatcga agaatgtctg aacggcttct
     3421 tcatacaaca gtgcaatttt gatgtcgaac tcgttatttc tgacgacaaa tcgaccgacg
     3481 gtactgtcaa ggtcattgag cgttgcttac agcgaaagcc tgataatttc accatcaggt
     3541 ttactgccca tgaaacaaat atgggagcag cacctaattt tgtctggtgt atggagcaat
     3601 gtgctggtaa atacatagcc ctctgcgaag gggacgacta ctggacagac ccacacaagt
     3661 tgcagaaaca gatggatttt ttggaggcga atgaggggta cagtttggta tgtggcgggt
     3721 atatgtcagt caatactgtg acaggtgaga aaaatgtagt tttgaaagaa tctccggatg
     3781 attccatgat gaccgatgcg ggttttgata taaccattga aatgtttttt caggattggt
     3841 acacgaaaac tttgactacg gtttttagga ctgaattatt agacttggga gaattggcca
     3901 attacagata caccagtgat acccaggtgt tttatcattt attgaagaaa ggaaatggtt
     3961 actatatacg cgatgtatta ggtgcataca atatgcatgg aagtggtatg catagcatgg
     4021 tctcgaatga aaaaagaagc cttgatggat ttagggtcgc taatgagtta tttgcacaaa
     4081 acaggaatga cacccttgta aggtcatatt atttttcttc aggacttgcg ttatatagta
     4141 taatgcctaa aattaaatat tatgagttta ataaaataga gttgttattt gatttaatag
     4201 tgcaaataaa aaattttgaa gaaatcaaaa ggcttttaat cgcggcatca gggaagcctg
     4261 gaagaaagct aattgattgg ctaaaaataa aatttaatct tgggaaaaaa tagttttagt
     4321 tgttgatcat aatactgaat taagtataat ttgattgttg agtatgataa caaagactaa
     4381 aataataaat agaattaatt taaaaaacac caaataatga tcaacgtaat atccccttac
     4441 aacgaacagg ctctgaaaga aggcagagca ttcaaattca gtcgccatta tcagttgtaa
     4501 tagatagcgg tgatggacac catcacttac ccaacagttt aagaaagtgg acaaataatg
     4561 aatcataatg tctggtacaa tttattgaac ataaaccagc ctccatgctc accctgatag
     4621 actacggcac cggcaacctg ctctctatcc aaaatatgtt tcgacgtatt ggagtaccgg
     4681 ttaagatttc gggcagcgct gaagatatcg ctcaggctga taaactgatt ctgccgggcg
     4741 tgggtcattt taaatatggt atgcaacaac tacagaaaag cgggctcatc gatgtgctcc
     4801 atgaacgtgt atgcctgcaa aaagtgccga tattgggtat ctgtctcggc gtacagctat
     4861 ttacccaaag cagtgaggaa ggcgacacac ccggacttgg ctggatagaa ggaaacaccg
     4921 ttgcatttaa caggaatagc cttgggaaaa atttaaaaat tccgcacatg ggctggtccg
     4981 aaatacatgg ctacgaaaaa tctgccttgt ttaccggcat gccacacgag ccacggtttt
     5041 actttgtaca ctcataccac ctgcaattaa aaagcgagtc cgacgccttg gcatacacag
     5101 actatggtta taaatttgca gcaggaattg aacatgacaa catactcgga gtgcagtttc
     5161 accctgagaa aagccacaaa ttcggcatga aactattgga aaacttcgtc aaatattatt
     5221 gatccacaga tggcagccat aagagtaatc cccagcttgc tgatacacaa agacggactg
     5281 gtgaagtcgg taagattcaa agattacaaa tacgttggag atccgattaa tgcagtcaaa
     5341 atcttcaatg ataaagaagt agatgaactt gtcatgatgg atatcgatgc gacgaaagaa
     5401 aaccgttcac cgaatataac cagaatagct gaaattgctg gagaagcctt tatgcctgtg
     5461 gcttatggcg gcggaataac aacagtgcag cagataaaag atatactttt caacggtatc
     5521 gaaaaggtca tcatcaacaa ggcagcacac acacagccgg agttgataac cgaggcagca
     5581 gccttgttcg gcagtcagac catcgtggta tcgatagacg taaagaaaaa cctgtttggt
     5641 aggtataaag tatataccga caatggcagg aataacacag gaatcgaccc ggcagttttc
     5701 gcaatacaag tggcgcaact gggcgcaggc gagatactcc tcaacagcat tgaccgggat
     5761 ggcacctatc aaggttacga cctgcccctg atcaagtcag ttagtgatgc cgtttctata
     5821 cccgtgatag ccatcggcgg agcatcatca gttgatgact tccaacaggc aataaacaac
     5881 ggagcgtctg ccacagcagc aggcagtatg ttcgtcttcc aacgcccaca tcaggcagtg
     5941 ttgatcagtt atccggatta tga
//
LOCUS       DAOQII010000151         5942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7603_length_5942_cov_3.789366, whole genome shotgun sequence.
ACCESSION   DAOQII010000151 DAOQII010000000
VERSION     DAOQII010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7603_length_5942_cov_3.789366"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..2013
                     /locus_tag="PLF48_07950"
     CDS             136..2013
                     /locus_tag="PLF48_07950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNF49294.1"
                     /translation="MAEVKYTEENIRSLDWKEHIRLRPGMYIGKLGDGSAPDDGIYVL
                     IKEIIDNSIDEYVMGYGKQIDIDIKEKIVTVRDYGRGIPLGAVIDCVSKINTGGKYDS
                     SAFQKSVGLNGVGTKAVNALSSYFKVESVRDGQKKTAEFDKGELIKDHKVEKTSDKNG
                     TKMQFNPDNTIFRNFHFVLEFVEEQLWNYAYLNAGLTINFNNNKYFSKDGLKDLLNKK
                     TNESELRYPIIHLKDKDIEIALTHSNQYGEEYYSFVNGQYTTQGGTHLAAFKEAIVKT
                     LRDFYKKQFEASDIRQAVIGAISIRVQEPVFESQTKTKLGSNVMWPADISRKIEEGPS
                     LKSFVNDFVKSQLDPYLHKNPTVADALLKRIQQSERERKELSGIRKLANERAKSANLH
                     NKKLRDCRFHYTDEKNPKHTDTTLFITEGDSASGSITKARNPETQAVFSLRGKPLNCY
                     GMTKKVVYQNEEFNLLQHALNIEDTIDDLRYNNIVIATDADVDGMHIRLLLMTFFLQF
                     FPDLVKKGHLYVLITPLFRVRDKKETVYCYSEKEKQAAINKLKGKPEITRFKGLGEIS
                     PHEFEAFIGNDMRLEPVVLKSNQSIEQLLEYFMGKNTPERQQFIINNLKVEVDEVELV
                     S"
     gene            2131..2856
                     /locus_tag="PLF48_07955"
     CDS             2131..2856
                     /locus_tag="PLF48_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNF49295.1"
                     /translation="MSIKNHIPNSLTLLNLFCGCCGIVACLYDQYLAVPVFIGIALLA
                     DFLDGFVARALKVKSDLGAQLDSLADMTTFGVLPGMMLFVLIRQAYTDIACTGYQGKY
                     FPYIGFIYSIFACLRLAKFNIDTRQTVNFIGLNTPSGAMFVLGLYANLKFHIFQIPFL
                     YSTTALLIIAVGLAFMLVSELEIFSMKGNPFSWKQNKWRVLFLISCIPQLIFLKWLGL
                     TTCIVSYVLFALMHNATKQPESN"
     gene            complement(2853..3749)
                     /locus_tag="PLF48_07960"
     CDS             complement(2853..3749)
                     /locus_tag="PLF48_07960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF49296.1"
                     /translation="MSAIPILPCISWNDYELIDSGNFEKLERFGQYITIRPEPQAVWD
                     KHLTATDWNTQAHVRFVPQSSSNGNWDFLKKMPENWTLEFPLTAKKNLTFNLALTKFK
                     HVGIFPEQSVNWKFIYEKASAIQAITNDCKVLNLFAYTGGASLAARATHAETYHVDSI
                     KQVVSWANRNMESSQQHDIRWVVEDAVKFVKREVKRGKKYNGIILDPPAYGHGANGEK
                     WKLEDQINEMILDTLSLLDKEHHFYVLNTYSLGFSALIIENLLSDNLKKAGITNIQPE
                     IAEIYIPSTTNQKLPLGVCARC"
     gene            complement(3755..4279)
                     /locus_tag="PLF48_07965"
     CDS             complement(3755..4279)
                     /locus_tag="PLF48_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNF49297.1"
                     /translation="METLDSQLKESPGNNSNAVIKLGVISGLILVLISFILFFTNSTY
                     EPWAKWISTAVMFCLIVFSQKILSDGFDKISFGKIFGMGFKITLIITLLSMLYMVIYT
                     NVLDVNFNEKVVQVARESMEKKGTLSEEQIEAALEMSKKFMTPAFMYIMLIVSNLIFG
                     SLFSVIGAAVFKKE"
     gene            4468..4938
                     /locus_tag="PLF48_07970"
     CDS             4468..4938
                     /locus_tag="PLF48_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49298.1"
                     /translation="MKFKLFILFLIFTVLLNAQSKVFKGAWFDIQYPASFTEVCSLKS
                     ISSDGCESAFFKSSDGLVEFYIFSPQWKAAYTDIALKSTEKQTQSKIVHKCSKTIKFW
                     TIVDKSGQYTRSYQEIADESTNWVIGLKYKNQAAYNKYKSAYLAFKKSLKQYAD"
     gene            4953..5540
                     /locus_tag="PLF48_07975"
     CDS             4953..5540
                     /locus_tag="PLF48_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49299.1"
                     /translation="MELLNNENVILESGNNSNLKVILTDLRIMIYTNLSFIGNNNTKE
                     YVFHEDVSKIETSYVSKKIYLIIAGILVLFGISMFRYMFLNIYRLIILIFLVSLFIFF
                     YFFSRAHYLNIYTNGSTKLGFSIVNKSDQEVSEIIKLFQQAKFNRVTNLRGNNSKTVT
                     AQVVNLNSNKEYCRFCNNKIKENDIYCENCGNKII"
     gene            5543..>5942
                     /locus_tag="PLF48_07980"
     CDS             5543..>5942
                     /locus_tag="PLF48_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49300.1"
                     /translation="MYVYKCISVPEIVNTGNTGKNSHNAAINHYEKLINSLAVDGWEL
                     DMIDSVSSLQNPGCLGQLIGIFTGGAKSEMVTFKLLIFRKQNSSKDTGYVQSQKIEIP
                     KVSSVNVVSKFNTCPSCSNPIRQEDIFCENC"
BASE COUNT         1997 a          960 c         1089 g         1896 t
ORIGIN      
        1 aggtgcattt atatttggat gaagccggaa aattcagcaa tgaagaaatt gaaaagacca
       61 ttaaccaaca attagacctt ttcctcgaat tttaaagaaa atagttcata acttacaaac
      121 caactgaaaa ttaaaatggc agaagtaaaa tataccgaag aaaacatacg ctcactcgac
      181 tggaaagaac atatccgctt acgtcccggt atgtatatcg gcaaactcgg cgacggtagc
      241 gcacccgatg acggcattta tgtactgatt aaagaaatta ttgacaactc catagatgag
      301 tatgtgatgg ggtatggcaa acaaattgat atcgacataa aagaaaaaat tgtaacagta
      361 agagattatg gtcgcggtat accactaggt gcggtaattg attgcgtttc taaaataaat
      421 acgggcggta aatacgattc ttctgcattt cagaaatctg taggtttgaa tggtgtgggt
      481 acaaaagcag tgaatgcttt gtcttcttat tttaaagttg aatctgtacg cgacgggcaa
      541 aaaaagacgg cagaatttga caaaggcgaa ttgataaaag accataaagt agaaaaaaca
      601 tccgataaaa atggcacgaa aatgcagttt aatccggata ataccatttt cagaaatttt
      661 cattttgtac tagaatttgt tgaagagcaa ttatggaatt atgcctatct caatgccgga
      721 ttgaccatca atttcaacaa caataaatat ttttcaaaag atggattaaa agatttgttg
      781 aataagaaaa ccaatgaatc tgaattgcgt tatcccatta ttcatttgaa ggataaggat
      841 attgaaatcg cactcacgca cagtaatcaa tacggggagg aatactacag cttcgtcaac
      901 gggcaatata ccacacaagg tggtacacat ttggctgcat ttaaagaagc gatagtaaaa
      961 acattgcgcg atttttataa aaagcaattc gaggcatcgg atatccgtca ggcagtaatc
     1021 ggggcaattt ctataagagt acaagagccc gtttttgaat cgcaaactaa aactaagtta
     1081 ggttccaatg tgatgtggcc ggcggacatc agccggaaaa tagaggaggg accaagtttg
     1141 aaaagttttg tcaatgattt tgtaaaaagt cagctcgatc catatttaca taaaaatcct
     1201 acggtagcag atgcactgtt aaaacgcatt caacagtcgg agagggagcg caaagaatta
     1261 agcggtatac gaaaactggc taatgaaaga gctaaatcgg cgaacttgca caacaaaaaa
     1321 ttgcgcgatt gccgttttca ctatacagac gaaaaaaatc cgaaacatac cgataccaca
     1381 ttgtttatca ccgagggaga ttctgcaagt ggttctatta ccaaagccag gaatccagaa
     1441 acacaagcgg tgttttcatt gcgtggtaag ccacttaatt gctatggtat gaccaaaaaa
     1501 gtggtctatc aaaatgaaga atttaatttg ctgcaacatg ccttaaacat tgaagatact
     1561 attgatgatt tgcgctataa taatattgtg attgctactg atgccgatgt ggatggtatg
     1621 catattcgct tattgctgat gacattcttt ttgcagttct ttccggattt ggtaaaaaaa
     1681 ggacatctct atgtattaat aacgccattg ttcagagtgc gtgataaaaa ggaaaccgtt
     1741 tactgttatt ctgaaaaaga aaaacaagcc gccatcaaca agctaaaagg caagccggaa
     1801 ataacccgat ttaaaggctt gggtgaaata tcgccgcacg aatttgaagc ctttattggc
     1861 aatgacatgc gattagagcc ggttgttttg aaaagtaatc agagcataga acaattgctg
     1921 gaatatttta tgggaaaaaa tacgcccgaa cgccaacagt ttattatcaa taacctgaaa
     1981 gtagaagtag atgaagttga actggtgagc tagttgaaaa agggaatgct taatgtccac
     2041 agttcatagt ccacagttat aagtatcaga ccatttccca aattcaaata acaattcact
     2101 aatcaccaat cactaatcac caatgcaaca atgagcatta aaaatcacat ccccaattcg
     2161 cttacgctgc ttaatttatt ttgcggttgt tgtggaatag tggcttgttt atacgatcaa
     2221 tatctggctg tacccgtttt tatcggcatt gctttattag ccgacttttt agatggattt
     2281 gttgcaagag cattaaaagt aaaatcagat ttgggagcac agttagactc gctggcagac
     2341 atgactactt ttggtgtttt gcccggcatg atgttgtttg ttttaatccg acaagcttat
     2401 actgatattg cctgtacagg ttatcaagga aaatacttcc cgtatatcgg ttttatttat
     2461 tccatttttg cttgtttgcg tttggctaag tttaatatcg acacacggca aacggttaat
     2521 tttattggac taaatacacc ttcaggtgct atgtttgtat tgggtttata tgctaattta
     2581 aaatttcata tcttccaaat tccattttta tattctacta cagcactgct gataattgct
     2641 gtcggattgg catttatgtt ggtcagcgaa ttggaaattt tcagcatgaa aggcaaccct
     2701 ttttcatgga aacaaaacaa atggcgagta ctctttttga taagttgtat cccacagtta
     2761 atcttcttga aatggttggg attgactact tgcattgtat catatgtctt atttgccttg
     2821 atgcataatg caaccaagca acccgaaagc aattagcatc ttgcacaaac gcccaacggt
     2881 aatttttgat tagtagtaga tgggatgtat atttcagcta tctccggttg aatgtttgta
     2941 atacccgctt tcttcagatt atctgatagc agattttcaa taattaatgc cgaaaatcca
     3001 agtgaatagg tattcaatac atagaaatga tgttccttat ccaaaagaga aagtgtgtcc
     3061 aaaatcattt cgtttatttg gtcttccagt ttccattttt cgccgttggc accatgtccg
     3121 tatgccgggg gatctaaaat aatgccgttg tattttttac ctcttttcac ttcccgcttt
     3181 acaaatttca cggcatcttc taccacccaa cgaatatcat gctgctgcga agattccata
     3241 ttgcggttag cccagctaac tacttgtttt atggaatcta catggtaagt ttcggcgtgg
     3301 gtggcgcgag ccgccaggct cgcgccgcct gtataagcaa ataaattcaa tactttgcaa
     3361 tcgtttgtta ttgcctgaat ggcacttgct ttttcgtaga taaatttcca attgacactc
     3421 tgttccggaa aaatgccgac atgtttaaac ttggtaagtg ctaaattaaa agtcagattt
     3481 ttttttgcag ttaacggaaa ttccaatgtc cagttttccg gcattttttt taaaaagtcc
     3541 cagttgccgt tggatgaact ttgcggtaca aaacgaacat gtgcttgtgt attccagtcg
     3601 gtagctgtca gatgtttatc ccataccgct tgcggttccg gacgaatggt aatatattga
     3661 ccaaatcgtt ccagcttttc aaaattaccg gagtcaatca attcataatc attccaactt
     3721 atacatggca aaataggtat ggcagacata agcattactc ttttttaaac acagcagcgc
     3781 cgattacaga aaataagcta ccaaaaatta aattggatac aatgagcatg atgtacataa
     3841 aagcaggtgt catgaatttt tttgacattt ccaatgctgc ttctatttgt tcttcactta
     3901 aagtgccttt tttttccatt gattcacgtg ctacttgtac tactttttca ttaaaattta
     3961 catctaatac gttggtataa attaccatgt atagcatact tagcaatgtg atgattaatg
     4021 tgattttaaa acccatacca aatattttcc cgaaagaaat tttgtcaaaa ccatctgaga
     4081 gaattttttg agagaaaaca attaagcaaa acataacggc tgtacttatc cattttgccc
     4141 agggttcata tgttgaattt gtaaaaaata agataaacga tatcaaaacg agtatcaagc
     4201 ccgaaatgac acctaatttt atcacggcat tgctattatt gcccggactt tcttttaatt
     4261 gtgaatctag agtttccatt atttagaatt tttggaaaga tacggagtta acaacaaaat
     4321 ttataaaaat atttcgggat aaatacttct gcgatcttta ttaatacagc taatatctga
     4381 ttaagtatgc attttcaaga atggatattt tttgactaga aattttacaa ttgttgaagt
     4441 ataatccgta tatttgtagt aagtgccatg aaatttaaac tatttatttt atttttaatt
     4501 tttactgtat tacttaatgc acaatccaaa gtttttaaag gagcttggtt tgatattcaa
     4561 tatccggcaa gctttactga agtatgctct ctgaaatcaa ttagttctga tggttgtgag
     4621 agtgcatttt ttaaatcctc agatggcttg gtcgaatttt atattttttc accccaatgg
     4681 aaagcagcat atacagatat tgcattaaaa tctacagaaa agcaaacaca gtcgaaaata
     4741 gtacacaagt gttctaaaac gattaaattt tggactattg tagataaatc aggacaatat
     4801 acaagaagtt atcaggaaat tgctgatgaa agcacgaatt gggtaatcgg attaaagtat
     4861 aaaaaccaag cagcttataa taaatataaa tctgcttatc tggcttttaa aaaatcattg
     4921 aaacaatatg cggattaaat taaaatagtc ttatggaact cttgaataat gaaaatgtga
     4981 tacttgaatc aggaaataac tctaatttga aggtaattct tacagatttg aggattatga
     5041 tttatacgaa cctatctttt attggtaata ataatactaa agaatatgtt tttcacgaag
     5101 atgtttcaaa aattgaaaca agttatgtaa gtaagaaaat ctatcttatt attgcaggta
     5161 ttctagtatt atttggtata agtatgttta gatatatgtt tttaaatata tatagattga
     5221 ttatacttat atttttggta tctttattta tcttttttta ctttttttcc agagcgcact
     5281 atttgaatat ttatacaaat ggaagtacta aactaggatt ttcaatagtc aataaaagtg
     5341 atcaagaggt atctgaaatt attaaacttt ttcaacaagc aaaatttaac agggtcacta
     5401 atctaagagg taataatagt aaaactgtaa ctgctcaagt tgttaatttg aattcaaata
     5461 aagagtattg tcggttttgt aataataaga ttaaagaaaa tgatatctat tgtgagaatt
     5521 gtggaaataa aattatttaa atatgtatgt atataaatgt ataagtgttc ctgagattgt
     5581 taatacagga aacacgggaa agaattcgca taatgcagcc attaaccact atgaaaaact
     5641 aataaatagt ttagctgttg acggttggga acttgacatg atagattctg tatcttcttt
     5701 acagaaccca ggttgtcttg gacagctaat tggaattttt acaggtggcg caaagtcaga
     5761 aatggtaaca tttaaactct taatatttag gaaacaaaat agttcaaagg atactggtta
     5821 tgttcaaagt caaaaaatag aaatacctaa ggttagttca gtaaatgttg ttagtaaatt
     5881 taatacttgc ccgtcatgta gtaatccaat tcgtcaggaa gatatcttct gtgaaaattg
     5941 cg
//
LOCUS       DAOQII010000152         5931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7627_length_5931_cov_6.181586, whole genome shotgun sequence.
ACCESSION   DAOQII010000152 DAOQII010000000
VERSION     DAOQII010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7627_length_5931_cov_6.181586"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..1455
                     /locus_tag="PLF48_07985"
     CDS             433..1455
                     /locus_tag="PLF48_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNF49301.1"
                     /translation="MSYAKLEYIWLDGYKPTQSLRSKTRIERNFSGKLEDCPVWCFDG
                     SSTEQAPGGSSDCLLKPVFICPDPDRKFGYLVMCEVMNPDGTPHVSNGRATIDDDDND
                     FWFGFEQEYFIWNPKTNKPIGWGEYTTTGEPGPQGPYYCSVGAGKAVGRFIVEEHLDL
                     CLEAGLNIEGINAEVAIGQWEFQVFAKGAKQAGDQTWVARYLAERTAEKYNYAINWHC
                     KPLGDTDWNGSGMHANFSNDVLRNCGSKATYEVVCEAFRPFVKEHIAVYGADNHLRLT
                     GKHETQSIDKFSFGVSDRGASIRIPIATVDKGWKGWLEDRRPNSAADPYKVAARIVKT
                     VKTAEV"
     gene            complement(1651..2811)
                     /locus_tag="PLF48_07990"
     CDS             complement(1651..2811)
                     /locus_tag="PLF48_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49302.1"
                     /translation="MKNLFWTLLIMLVAFTQTNAGFINGTMTHQNKVRKYVIYVPSLY
                     YSQNKKVPLLIGLHGFGDDIANFSQICMSNIADTANYIVAYPEALADPVLGANAWNSG
                     AGALGIIVNNTIDDVGFITRLMDTIISKYSIDTTRMYMFGFSFGGFMTNRMACEKSSR
                     LAAVASVSGLKGNYVTATPQVPMPYMHFHGTADQTIGYYGTSTMGFLPGLGLSAENTT
                     KYWANHNHCNMTPIVDSMPNLKNDGLRFVRFSYLGGDNGSTAILYKVMNGEHKWYGTP
                     TNDISYCQTIWSFFSQYSRTATITAIRNNSSKTSIKIFPNPSSGSISIQSEAAVNSVQ
                     IWNMEGKMIVEDKIDNQRAFTLSKDLSNGAYIVRLLNNKNETLGEEKLLIQH"
     gene            complement(2808..3506)
                     /locus_tag="PLF48_07995"
     CDS             complement(2808..3506)
                     /locus_tag="PLF48_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNF49303.1"
                     /translation="MLALLLYLLDGVIHLISEYLHFPIVKYGSKILLMPLLALFLVSK
                     VKSNNKLILSALFFSWLGDIFLMFPRNEYSEQMQKLLFIFGLASFLIAHVFYIFTFLN
                     EINSVKKVSLIVENPYLVLPFLVFLFFLLYFLFPYLKDMKLPVVVYGSIINLMALMAL
                     NRKNLVIASSFYLVFIGALLFMISDTTIAVNVFYKAQPWHRIFIMSTYILAQALIVYG
                     WVQNQVLSNKSLKI"
     gene            3573..4376
                     /gene="proC"
                     /locus_tag="PLF48_08000"
     CDS             3573..4376
                     /gene="proC"
                     /locus_tag="PLF48_08000"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNF49304.1"
                     /translation="MNVLIVGGGNMGRSFAQSFLNAKILTHKQLTIIEKDAAQIAELN
                     RIKLGQVYNEFGDFITEKDLIIIAVKPQDAHAIYPKLSPFINEKHIILTIMAGVSIAT
                     LEEHLQTKKVVRAMPNLPCQIGMGVTGFMVSEKISQYDVDFIRKLLETTGGTIQVYDE
                     DKLNAITAISGSGPAYVFYFMDAMINKSMEMGFSHTEASKIVEQTFLGAVELYKVNNL
                     SCKEWIEKVASKGGTTEAAIRQFNQQTVSENIELGLEKALLRSRELSKN"
     gene            4391..5857
                     /locus_tag="PLF48_08005"
     CDS             4391..5857
                     /locus_tag="PLF48_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49305.1"
                     /translation="MRKLVGLFVGMISIFAQAQNFDFSPNCKQAYQSVFKLKINDGLT
                     FLSSEKKQNPKNLFPYFIENYVDFFKVYINDNDVQYNQFMSKAEAHLNKLKTGDKSSP
                     YYLYTQAEIYFQQALIKLKFGKYMSAVFDIKKSNELLEKNKTKFPAFLPNQKSLGLLH
                     IIFGNIPDNYKFGASLLGLKGSVNLGVQELKQVLQDENFPFREEALIEYILIQLHVEK
                     NKEAAWQWVADMKLPTDDNLLNSFIAASVASYNGKSDKVLELLAKRPTGKAFMAVPYL
                     DLLYGTTKLSRLDADADVYIKSFLQQNKGRSYQKEANRKLAWFYLVNGKPDLYKKYMQ
                     TVLSIKDAPTDEDKAAQHEAESGYMPNAQLLKARILNDGAYYDKALMVLNEIKPEKFT
                     RNKDKIEYLYRRARIYDDMGKTDNAVSFYLQTIDKGKNLDYYYIANACIKLGAIFERQ
                     NKNASAKKYYQMALDMDKDEYENSINAEAKAGLNRIDK"
BASE COUNT         2022 a         1065 c         1157 g         1687 t
ORIGIN      
        1 ttacaataat tttcattggc tattttttat gttgatagta atgacaatgc gaatatcaag
       61 catattacaa tgcccgaaat taacaaaaaa acagacaaaa atgatgcata ctattttcgg
      121 aagcatctct taaggtgagt tttttagaat tagtaaacga catatgtaaa ttacttcgat
      181 tcattttttc gccttcttct tactttttac taccaattca gctgcataaa aataaaatca
      241 gtcgaataat gctaaatgag atggactaaa gcgttttttt tagttgaaat aacagacttc
      301 ataatctgtt ataaaaacac ataataatct cttaaactaa aattaattag aaaattcttt
      361 gaaaatttca atcaaaatct aaactttaca taaaataatt aaaaaatttt tcacatttta
      421 aaacaattta atatgtctta cgcaaaatta gagtacattt ggctagacgg atataaaccg
      481 acacaaagct tacgcagcaa aacaagaata gaaaggaact tcagtggcaa actggaagat
      541 tgtccggtat ggtgttttga cggctcttct acagagcagg cgccgggtgg ttcgtctgac
      601 tgtttattaa agccagtttt tatttgtccg gatccggata gaaaatttgg ttatctggtg
      661 atgtgcgaag taatgaatcc tgacggaacg ccacatgtat ctaatggcag agccaccatc
      721 gatgatgatg ataatgattt ttggtttggg tttgaacaag agtatttcat ttggaatcca
      781 aaaacaaaca agcctattgg ctggggcgaa tatactacaa ccggggagcc cggcccacaa
      841 ggtccttact attgttctgt aggtgcaggc aaggcagtag gtcgttttat agtggaagaa
      901 catctggatt tgtgtttgga agcaggatta aacattgaag ggattaatgc agaagttgcc
      961 attggacaat gggagtttca ggtgtttgcc aaaggagcta agcaggcagg cgatcaaaca
     1021 tgggtggctc gatatctggc tgagcgtacg gctgaaaaat acaattatgc aatcaactgg
     1081 cattgcaaac cgctgggcga tacagactgg aatggttctg gtatgcatgc caacttctct
     1141 aatgatgttt tgagaaattg cggaagcaaa gcaacctacg aggtggtatg cgaggctttc
     1201 cgtccgttcg tgaaagagca tatcgccgtt tatggtgctg acaatcattt gcgcttaacc
     1261 ggaaaacacg agacacaatc tatagataaa ttttcttttg gcgtgagcga cagaggggca
     1321 tctatacgta ttccgattgc cacggtagat aaaggctgga aaggctggct ggaagaccgc
     1381 agacccaatt ctgcagctga cccgtataaa gtagcagcac gcattgtaaa aacagtgaaa
     1441 acagcagaag tttaaaaaat tgctttttgc acaatacagg aaagggacgg cttttatgct
     1501 gtcccttatt gtttatttgc aactatccgt ttgccactca gtatattatg tcaattatct
     1561 tctccttttt gccctttatt cctctacacc tccaatatag acaaaggctt ttgcttggtt
     1621 tagatacaac tattcctggc aaaaatcata ttaatgctgt attaacaact tttcttcccc
     1681 tagtgtttca tttttattat taagtaatct cacaatatac gcaccgtttg ataaatcttt
     1741 agaaagtgta aacgctcgtt ggttatctat tttgtcttct actatcattt tgccttccat
     1801 attccaaatt tgcactgagt ttacagctgc ttcagactgt attgagatac ttcctgatga
     1861 tggattagga aaaattttaa tgctcgtttt tgacgaatta tttctaatgg cagtaattgt
     1921 cgctgttcta gaatattggc taaagaaact ccatattgtt tggcaataag aaatgtcgtt
     1981 tgtaggcgta ccataccact tatgttcacc attcatcact ttatataaaa tagcagtact
     2041 gccattatca ccgcctaaat acgagaagcg gacaaaacgc aagccatcat ttttaagatt
     2101 tggcatagaa tcaactatag gagtcatatt gcaatgatta tggttagccc aatatttcgt
     2161 agtgttttca gcacttaatc ccaagcccgg caagaaaccc attgtagatg tgccataata
     2221 gccaatagtt tggtcggcag tgccatgaaa atgcatatag ggcatgggca cctgcggagt
     2281 ggcagttaca taattacctt tcaaccctga aacagaagca acagcagcca agcgagagct
     2341 tttttcacag gccatgcgat ttgtcataaa cccaccaaaa gaaaaaccaa acatatacat
     2401 gcgtgtcgtg tcaatggagt attttgagat aatcgtatcc atcagtcggg ttataaaacc
     2461 gacatcatct atagtattat ttactataat gcctaaagca ccggcaccac tgttccaagc
     2521 attagcccct aaaaccggat ctgctaaagc ctctggatag gcaacaatgt agttagccgt
     2581 atcagcaata ttagacatgc aaatctgact aaagttggca atatcatcac caaagccatg
     2641 caatccaatc agcaaaggca ctttcttgtt ttgagaataa tataaggacg gaacataaat
     2701 aacatacttc ctcactttgt tttgatgcgt catggtgcca ttgatgaagc cggcatttgt
     2761 ttgagtaaat gcgacaagca tgattagtaa tgtccaaaaa agatttttca tattttaagc
     2821 gatttatttg ataaaacttg attttgcacc catccgtaaa caatcaaagc ctgtgctaat
     2881 atataggtgc tcataataaa tatccgatgc catggttgtg ctttataaaa cacatttacg
     2941 gctatggttg tatcagaaat cataaaaagt aatgccccga taaaaactaa ataaaaggaa
     3001 gaagctatga ctaaattctt ccggtttaat gccatcaatg ccatcaggtt aatgatgctt
     3061 ccataaacaa ctacaggcaa tttcatgtct ttcagatagg gaaacaagaa ataaagcaag
     3121 aaaaacaaga aaactaaaaa gggaagtacc aaatatggat tttcaacgat taaagatact
     3181 ttcttaacgc tgtttatttc atttaagaaa gtgaaaatat aaaagacatg cgcaatcaag
     3241 aaagaggcta aaccaaaaat aaatagtagt ttttgcattt gctcactata ttcattacgc
     3301 ggaaacatca aaaaaatatc gcctagccaa gaaaaaaaca atgcagataa aatgagcttg
     3361 ttatttgatt ttacttttga aaccagaaag agcgcaagca agggcatcag caaaatttta
     3421 ctgccatatt ttactatcgg gaagtgcaga tattctgaaa taagatgaat aacaccgtca
     3481 agcagataaa gcagcagagc aagcatctat aaaaatacac attctacact aaaatggtga
     3541 tgcatgacac gaaaaaaaat tatcttcata ccatgaatgt attaattgta ggcggaggca
     3601 atatgggaag aagttttgcc caaagctttt tgaacgctaa aattttaacg cataagcaac
     3661 ttactattat cgaaaaagat gctgcacaga ttgcagaact taaccgcatt aagcttggtc
     3721 aggtttacaa tgaattcgga gattttatca cagagaaaga tttgattatt attgcagtca
     3781 aaccgcagga tgcacatgct atttatccca aactgagccc ttttataaac gaaaaacata
     3841 tcatcctgac cattatggca ggcgtttcta tcgcaacttt ggaagaacat ctccaaacga
     3901 agaaagtggt gcgtgcgatg ccgaatttgc cttgccaaat tggaatgggc gtaacgggat
     3961 ttatggtgtc agaaaaaatc agccaatatg atgtcgattt tatccgcaag ctgctggaaa
     4021 ctacaggtgg cacgatacaa gtttacgatg aagataagct gaatgccatc acggccattt
     4081 caggaagcgg acctgcgtat gtattttact ttatggatgc catgataaat aaatcaatgg
     4141 aaatgggctt ttcgcataca gaggcctcga aaattgtaga acaaaccttc ttgggtgctg
     4201 ttgaattgta taaagtcaac aacctctcat gtaaggagtg gatagaaaaa gtggcatcta
     4261 aaggtggaac aacagaggca gccatccggc agtttaacca gcaaacggtt tctgaaaaca
     4321 ttgaattggg tttggaaaaa gcgctgttgc gatctagaga attaagtaaa aactaaaaag
     4381 tggataggtc atgcggaaat tagtgggttt gtttgttggt atgatttcca tttttgctca
     4441 ggcacaaaat tttgattttt cgcccaactg caagcaggct tatcaatctg tttttaaatt
     4501 gaaaataaat gacggcttga cttttctttc atctgaaaaa aaacaaaatc ccaaaaattt
     4561 attcccttat tttattgaaa actatgttga cttttttaag gtgtatatca atgataatga
     4621 tgtgcaatac aaccagttta tgtccaaggc agaagcgcat ttgaataagc tgaaaaccgg
     4681 cgataaaagc tctccttact atttatatac ccaggcggaa atatattttc agcaagcact
     4741 tattaaatta aaattcggca agtatatgag tgcagtgttc gatataaaaa aatcgaacga
     4801 attactcgaa aaaaacaaaa ccaaatttcc cgccttctta cccaatcaaa aaagcctggg
     4861 actactgcat attatttttg gcaatatccc cgataattat aaattcggcg catctctgct
     4921 tgggctgaaa ggctcagtca atctgggtgt tcaagaactt aagcaagtct tgcaggacga
     4981 aaattttcct tttcgggaag aagctcttat agagtatata ttgatacagc tgcatgtaga
     5041 aaaaaacaaa gaagcagcct ggcaatgggt agccgatatg aagttgccaa cggatgataa
     5101 cttactgaat agttttatag cagcatccgt agcatcttat aatggcaaaa gcgacaaagt
     5161 gctggaattg ctggcgaagc gaccgacagg aaaagcgttt atggctgttc cttatttgga
     5221 tttattatac ggcactacca aactttcgcg cttagatgcc gatgccgatg tgtatattaa
     5281 atctttcttg caacaaaaca aaggacgttc ataccaaaaa gaagccaata gaaaattagc
     5341 ctggttttat ctggttaatg gcaaacctga tttatataaa aaatatatgc aaacggtgtt
     5401 gtcaataaaa gatgcaccta ccgatgaaga taaagcagcg caacacgaag ccgaatccgg
     5461 ctatatgccg aatgcgcaat tgctgaaagc cagaatactg aatgacggcg cttattatga
     5521 taaggccttg atggtattga atgagataaa gccggaaaaa tttacgcgca ataaagataa
     5581 aattgaatac ttgtaccgaa gggcacgtat ttatgatgat atgggaaaaa cagataatgc
     5641 tgttagtttt tatttgcaga ccatagataa aggaaaaaat ttagattact actacattgc
     5701 taatgcttgt ataaaattag gggctatctt tgaacggcaa aataaaaatg caagtgcaaa
     5761 gaaatactac cagatggcat tggatatgga caaagatgaa tatgaaaaca gcataaatgc
     5821 cgaagcaaaa gccggactaa acagaataga taagtaagct gcaactaaaa tatgctcagg
     5881 gcatgcacag caatacccca ctctcaggat gtgttttcga ttagcatagc c
//
LOCUS       DAOQII010000153         5930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7628_length_5930_cov_4.804426, whole genome shotgun sequence.
ACCESSION   DAOQII010000153 DAOQII010000000
VERSION     DAOQII010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7628_length_5930_cov_4.804426"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(606..1385)
                     /locus_tag="PLF48_08010"
     CDS             complement(606..1385)
                     /locus_tag="PLF48_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49306.1"
                     /translation="MKTNIITKLIQLFKPNKDWQSEPTNKDAISTINGIEITYESEAF
                     RIESLGSIYFSIQGISQEKLVELLQFENIRKLNAKDATDYLYNDEDFGKKKTDSSTLK
                     AYVTCELNSWVYLKWESGIFEVNKHLIEYLMKYTTGVIAYFYVDPYVDGYEWIIAQNN
                     NIIRAFKYDMGNLYIDEGKPVCYEEIEFLRLREKEKEKSFEEWIRTRENPFVSCEEVY
                     AGVVKNTSQHIEELNKDLSSLGDFYVGTVNLKKLQGTTSNS"
     gene            complement(1563..2051)
                     /locus_tag="PLF48_08015"
     CDS             complement(1563..2051)
                     /locus_tag="PLF48_08015"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HNF49307.1"
                     /translation="MQEELLDDQFKEEEYPNAQDYDHSLHGLNGWLAFLGFSLIASMV
                     LTAVGNFKNYATIFRQFSLFEPKFKFWLGVEFILILGVFIMQIVVLINYFGKKKLFPK
                     LFLIYLGIYLAVSLFDNLVIVKVLSGEFAIAGIIRPVIYALVWDLYITNSKQVNHTFL
                     YK"
     gene            complement(2071..3045)
                     /locus_tag="PLF48_08020"
     CDS             complement(2071..3045)
                     /locus_tag="PLF48_08020"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNF49308.1"
                     /translation="MPFLPKQNQLIENGKLLPFGIYQDIIDNTDTSYFDEKGSLLAKN
                     RRTERKAWIFFGVYSPEIICGIAVVDAGMAATAFCYFYSLKDSTFIEDKLTVPFGFEQ
                     AFNPNLYTDFKLKNYSISHKNGKWKMEYNGKFNLIIESENNANGFSVVSPSQNRPFNF
                     TYKNCCLPVSVQINAGTNKTYAVSGNYGCIDYTKGYPPRETRWNWLSFIGITENKEPV
                     GVNLVDKFNNNMENVLWLNGEKIILSEAEFSMQKPLDKSDWLIATRDGILSCHMSPEG
                     ARSENINALILKSNFTQPFGRIDGTITLNGITQKFTAFGVAEDHYAYW"
     gene            complement(3100..4371)
                     /locus_tag="PLF48_08025"
     CDS             complement(3100..4371)
                     /locus_tag="PLF48_08025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNF49309.1"
                     /translation="MNYNNLHTHLEARVLFIDMNSFFASCEQQTNYWLRGRPVAVCVY
                     TGKYGCVIAPSIEAKKRGIKLGLRLNEAMQICPDLVPLETNPEKYRSFHVKIMNVLRK
                     YSNEVIPKSIDEAVVDLTTYKKIYPDPIAVAHAIKNDIRNEVGDWLKCSIGIAPNAFL
                     AKLASDIQKPDGLTVIDPQNIDEVLGKLELTDLPGIAKAMSARLMKAGIYTPLQLRYS
                     KPEILKAACKSIIGLHWYYRLHFQEVDMLGTDEYKSMQAMRQVSKEQRENVGTLHEIM
                     MMLCLTLENRLVKKELYCKYVQFYVNYDDKTGWQDKFHTNQPLQDGMQIYHILQERQQ
                     SFEKKHEPLLPIINKHVTSIGLVVTEFINEMQALQLDIFDNSHIKNNKLRKAVYNIKD
                     KYGKSTILRASELKEDEFTHDIIGFGNIKDL"
     gene            5426..5653
                     /locus_tag="PLF48_08030"
     CDS             5426..5653
                     /locus_tag="PLF48_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49310.1"
                     /translation="MSSTIDTLREKLHHYLEVVEDKKIQAIYTLFEEEIIDIEQYNKD
                     IEEAEKEIEAGNYYTHEQVLAEIKTWKKKAV"
BASE COUNT         1901 a         1081 c         1041 g         1907 t
ORIGIN      
        1 ggacaggaga ttttcattct tcaaacaggg gttcgattcc cctacgggct acaaaggcat
       61 cttcggatgc ctttttttat tgggttttgg atgtcaaatt tgatacaata gtattgctga
      121 ttttttctga aatcataaca gcataaaaag atatgatttt gctttggaga atttgtgata
      181 ttttgagaag gaaaaggtgg atgattagtt taacttatta taatccgata ttttagttta
      241 ttccattcgg aactcattcc gagtaaaaag atgagttgaa cgtatttgtc catattataa
      301 tctctgtttg attttttaat ccatttttca aaaaaaacat atcaaattga ggagtatcaa
      361 attcgggaaa acagaaatta aaacgtccca gtatttacta aaatcattac aattgagttc
      421 tagtgcaata ggagatagtt gggaggccat gtctcaggct gcgccagttt ttattttttt
      481 tattttacca tgcttggtct aggtaagggt tcaatacggt tcgtggttgg taggtgataa
      541 cacctgctaa aggcgaggta ttatagtatt tatttaattt ttatgtttta aatatattag
      601 tataatcatg aatttgatgt agtgccttgt agttttttca gattgactgt cccaacataa
      661 aaatcaccta aagaacttaa atctttattt aattcttcta tatgttggct ggtgttttta
      721 acaacgccag cataaacttc ttcacaagaa acaaatggat tttctcttgt tctgatccat
      781 tcctcaaaag atttctcttt ttctttttcc cttagcctta aaaactcaat ttcctcatag
      841 catacaggct taccttcatc tatatacaaa tttcccatgt catatttaaa tgctctgata
      901 atattattat tttgtgcaat tatccattca tatccgtcca cgtatgggtc aacatagaaa
      961 taggcaatta caccagtagt atatttcatc agatattcaa ttaagtgctt attaacttcg
     1021 aaaattcctg attcccattt taaataaacc cagctattta attcacatgt tacataggct
     1081 ttcaaagtag atgaatctgt cttttttttg ccaaaatctt cgtcattgta caaatagtct
     1141 gtagcatctt tggcattcaa tttcctgata ttctcaaatt gcagtagttc tactaacttt
     1201 tcctgactta tgccctgtat tgaaaaataa atgctgccta aagactcaat tctaaatgct
     1261 tctgattcat aggttatttc aattccattt attgttgaaa tggcatcttt attcgttggt
     1321 tcactttgcc aatctttatt tggtttaaac aactgtatga gtttagttat aatgtttgtt
     1381 ttcatgatta tcgcattttt cgttatttga atctatcaaa aaaatagctc tctattatta
     1441 ttattcaaca gtaaatgcat ttactaacta gtataatcga caaatctata tttatttaaa
     1501 taaaatataa ttaaaataat attatttata ctattttact actaaacatt tagagctaat
     1561 cttcacttat acagaaaagt atgatttact tgtttggagt tggtgatata taggtcccaa
     1621 actaatgcat aaattacagg tcttattata ccggctattg caaattcgcc gcttagtact
     1681 tttacaataa ctaaattgtc aaacaggctt acggcaagat agatgcctaa gtaaatcaag
     1741 aataattttg gaaatagttt ctttttgccg aaatagttaa tcaatacaac aatctgcatg
     1801 ataaacaccc caagaatgag aataaactca acacccaacc aaaacttaaa tttgggttca
     1861 aaaagtgaaa actgcctgaa tatggtcgca taattcttga aattacctac agcggtaagc
     1921 accattgacg caataagtga aaatcctaaa aatgccagcc agccattcaa tccatgcaag
     1981 ctgtggtcat aatcttgtgc attgggatat tcttcttcct taaactgatc gtctaataat
     2041 tcttcttgca tatactgtta ctcttatttt ttaccaataa gcataatggt cttcagcaac
     2101 accaaatgcc gtaaattttt gtgtaatgcc attcaatgtt atcgtgccat ctatcctgcc
     2161 aaaaggttgc gtgaaattgc tttttaaaat caaggcattt atattttcac ttcttgctcc
     2221 ttcgggcgac atgtggcaac ttaaaattcc atcacgcgtt gctatcagcc agtcactctt
     2281 gtctaatggt ttttgcatac tgaattccgc ttctgaaagt atgatttttt cgccgtttag
     2341 ccagagcaca ttttccatat tattattgaa tttgtcaacc agattaacgc caacaggctc
     2401 tttgttttca gtaatgccga taaaagaaag ccagttccat cttgtttctc tgggcgggta
     2461 gcctttcgta taatcaatgc atccgtaatt gccgcttaca gcatacgttt tattagtgcc
     2521 ggcattaatt tgcacactta caggcaagca acagttttta tacgtgaaat taaatgggcg
     2581 gttttgtgac ggcgacacca cgctgaagcc attcgcatta ttttcagatt ctataattaa
     2641 gttgaatttg ccgttgtatt ccattttcca ctttccgttt ttatgtgaaa tactgtaatt
     2701 tttcagtttg aaatcagtgt acagattcgg attaaaggcc tgctcaaatc caaagggaac
     2761 ggttagctta tcttcaataa atgtggaatc tttcagcgaa taaaaataac aaaaagccgt
     2821 ggctgccatg cctgcatcta ccacagcgat gccacaaatt atttcgggtg aatacacacc
     2881 gaagaaaatc caggcctttc gctctgttcg gcggtttttt gccagcaatg agcctttctc
     2941 atcaaaatag gaagtatcgg tattatcaat aatatcctga taaattccaa atggcagcag
     3001 tttgccgttt tctatcagtt ggttttgttt aggtaaaaat ggcataatta tttagttcga
     3061 taaaaaacga atatacgatt ttagttggat taattgaact tataaatctt tgatattgcc
     3121 gaagccgata atatcatgcg taaattcatc ttctttcagc tctgaggctc tcaggatggt
     3181 ggacttgccg tatttatctt tgatattata aactgctttg cgcaatttgt tattcttgat
     3241 atgcgagttg tcaaaaatat cgagttgtaa agcctgcatt tcattgataa actcggtaac
     3301 caccaaaccg atgctggtta catgcttatt gataatgggc aataaaggct cgtgtttttt
     3361 ttcaaaactc tgctgtcttt cctgcaagat gtgatagatt tgcatgccgt cttgcaaggg
     3421 ctgattggta tgaaatttat cttgccagcc ggttttatca tcatagttga cataaaactg
     3481 tacatattta cagtagagtt ctttttttac taaccgattt tccaacgtaa gacacaacat
     3541 catcataatt tcgtgtagcg tacccacatt ttccctttgc tctttagata cttgccgcat
     3601 ggcttgcatc gatttgtatt cgtcggtgcc cagcatgtcc acttcctgaa aatgcaggcg
     3661 gtaataccag tgtaacccaa taatgctttt gcaggccgcc ttaagtattt cgggcttgga
     3721 gtagcgcaat tgtaaaggcg tgtaaatgcc ggctttcatc aggcgtgccg acatggcttt
     3781 ggctatgccc ggcaaatcag tcagttccag tttgcccaat acttcatcta tattttgcgg
     3841 atcaatgacc gtcagcccgt cgggtttttg tatatcactg gccagtttag ccaaaaaggc
     3901 attgggtgct atgcctattg agcatttcag ccagtcgccc acttcatttc tgatgtcatt
     3961 ttttatggca tgtgccacgg caatggggtc gggatatatc ttcttatagg tagtcaaatc
     4021 caccaccgct tcatctatac ttttgggaat tacctcgtta gagtatttac gaagtacatt
     4081 catgatttta acatgaaaag agcggtattt ctccggattg gtttccagcg gtaccaggtc
     4141 agggcatatc tgcatggctt cattcaggcg caagcccagc ttgatgcctc tttttttagc
     4201 ctcgatggaa ggggcaatta cacagccgta ttttccggta tatacacaga cggcaacagg
     4261 tctgccacgt agccagtaat tggtttgctg ttcgcaactg gcaaaaaaac tgttcatgtc
     4321 aataaataag acacgagctt ccagatgcgt atgcagattg ttataattca ccctttcaag
     4381 ttacaaaaaa aatgcctgcc gaagttgtca tgtggaaatc tattttatga gctaatgact
     4441 tgagtttaga acagaccttt ctgataaata agttgatccc actctgacgc gagagttagc
     4501 gcagcgctct cgtgtctagc tcactacgtt cgccgttgat ttccccgccc ttggcaggtg
     4561 ttatcatctg ccaaccacga accgtattga aaccggaaaa tattttttaa tttcaagtat
     4621 gattaatgaa gaaaatcatc ctcaattttt tacccccgct cttggcaggt gttatcacct
     4681 gccaacccag tggctcgccg catagcaaca tcaacaaaca atttacatta aactaatttc
     4741 ttaaactaat ccaaatacgg aaaaattata attacaaaaa aggcaagcca ttaaagttta
     4801 gcccgaacaa taaacagcat gaggcgataa aaaatcgtgg cgacttaaaa ctatcgtgaa
     4861 acgacatgga atacactatg ttttgttgta taaagcctac cgaaaataaa cacaaacagc
     4921 gagtgacaag atagttttca caagcagatt ttttagagct gaagccaata gcacaggcaa
     4981 aaatgccttt tattgttctt gattttttgc tccacttttg tatcaagaca aaagtggaat
     5041 aatacaaaaa cacggcaaca ggtataacaa agccctattg gctcgccctt ggcaggtgtt
     5101 taagcgcagc tcagtgagta tttgatcaaa aactaaatta cattcataat aatccaggat
     5161 aaagcaggtt tatgcagttt accggaagag tatgtatatt cgagtgcgga ttattatgaa
     5221 ttaaataaaa gtaaatggga ttttatagcc aactatgatg aataataagg tatatttatg
     5281 gtatggtagc cggcaggtga taacacctgc cgagggcgag ggaatagtaa taaacgagat
     5341 agacaagtat cacccgatag gataagcgaa aattaacaac tatcaaacga taaaaagatt
     5401 aaatttggta gaaaatacaa tcatcatgag ttcaacaata gatactttaa gagaaaaatt
     5461 acatcattat ttagaagtag tggaagataa gaaaatacaa gctatttata cattgtttga
     5521 agaagaaata atagatatag aacaatacaa taaggatata gaagaagcag aaaaagaaat
     5581 agaagcagga aattattata cacatgagca agtattggca gaaataaaaa catggaaaaa
     5641 gaaagcagta tgatagaaat aatttgggca aaacgagcat taaccggatt acaaaaaata
     5701 tatgaatata tagaagaaga ctcaccacaa aacgcagaaa taatatcaat gaaataacag
     5761 aaaaagtaac agaattataa gagcaaccag aacgatataa actagacgaa tacaaaaaga
     5821 ataatagcgg aaattacaga gcgtttgaac ataagaagat acgcatatca tacaaaataa
     5881 atgaaaagca aatagacata gtacgagtac gacataccaa tagaaagccg
//
LOCUS       DAOQII010000154         5882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7713_length_5882_cov_4.719410, whole genome shotgun sequence.
ACCESSION   DAOQII010000154 DAOQII010000000
VERSION     DAOQII010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7713_length_5882_cov_4.719410"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..2677)
                     /locus_tag="PLF48_08035"
     CDS             complement(209..2677)
                     /locus_tag="PLF48_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF49311.1"
                     /translation="MKSFFFIIFSSILFSFAQPPASGRISGKVVDASSGKPLDYSSIT
                     IQHLKDTSTILGSLVDEKGNFEISAIPYSVYKLTVSFLGYKDYIIPALKVFPPDNIDV
                     KLGTLKVNEDAKVLDEVEIKAEKSMMTLNAEKKVFNVDKNTLSAGGTAIDALKQVPTL
                     NVDTDGNLENRGSTNLKIYINGRPSGITAGNTKAILEAIPANSIESIEVINNPSAKYE
                     AEGDIGIINIILKKSANKGLNGNFNIGYATKYDFNTGVTLNFRNEKVSTSTNYSIRYN
                     ESFYGGNSQRFNFPNGISPYYLLSKDRTTSDNINNTLTNTTDWYIKEKNTWSFSTLLS
                     NNLGFSNGKSQSNLTDSAAAFLIGFNRYTKDKSINYNAELTTSYRRTFKSNTHDLVLT
                     GNYAYVNRGRNPDYTQLYFDVNNTEYVQVPLIQSNTIKNKNHTAYFQADYTQPFEKAK
                     GKFETGYRASYRNLLNDFYADSINHTLNKTETDSSITNTFRYKELINAAYAIYGGNYK
                     KFSYKVGMRFEYSLVNGKQYVQAITNKQRYFDYFPSASFSLKLPKNNDISISYSKRIN
                     RPQPEQLNPFGSREDVLNILTGNPNLKPAYSHNLEFTHTASFKKGVFLTSTIYYRYAQ
                     NMFTRYRLVDSTGLSIVTFDNLNKGQSAGGEFTVRAGIFKWWNIMANVNLFYNQMKGT
                     VPPETTATSSRSFQYNFRIMNTFTFWENASLQFMVNYRSPFNYLQGKINHMVFASIGF
                     KKDILKNKQGTIAINVNDVFHTMYFGVNTAGSTFSGSTKRYWESTIGTITFTYRFGKN
                     DNKPTTPAKKKNNFEDSAGDVIGG"
     gene            complement(2740..3135)
                     /locus_tag="PLF48_08040"
     CDS             complement(2740..3135)
                     /locus_tag="PLF48_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNF49312.1"
                     /translation="MKKYAILATLFGMTAVMLGAFGAHGLRKFVDDHGLEIWNKGVQY
                     QFYHTFALFIAFFLFEKYALPAFKTASMLFCTGILLFSGSLYILACKDILQISESLLK
                     VIGPITPLGGLSFIAGWSFLLVGLLKTAK"
     gene            complement(3144..3545)
                     /locus_tag="PLF48_08045"
     CDS             complement(3144..3545)
                     /locus_tag="PLF48_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNF49313.1"
                     /translation="MKPDITTRNDIEILVAYFYGKLLSDEVLQNIFQNTVMEHLRKHL
                     GTIADFWDSILLDSNVYRGNVTEAHYQVDKHFPMNKTEFEHWLLHWKSSVDELYEGEK
                     AEMAKYRAQTIADIMQYKLDYVRKIREEQTE"
     gene            complement(3595..4536)
                     /locus_tag="PLF48_08050"
     CDS             complement(3595..4536)
                     /locus_tag="PLF48_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF49314.1"
                     /translation="MLKRALITAYIHPYLQQQLENSGWQVSVQPDITYEELQLIIDHF
                     SLLIITTYLRIDKNIIDHAQHLKIIGRVGSGMENVDMAYCIEKGISCFNSPEGNANAV
                     GEHALALLLALYNRITIANNELKLGIFNREYNRGEELDGKTVGIIGFGHTGAAFAKKL
                     MGFDVSILAYDPNKEIKQTNIIQTSLHHLQQEADIISFHVPYSAETHHYCNAAFLQTC
                     IKKPTIINTSRGAVCDTQAILTALRQQTIYGFCVDVFEDEPLTKNKINPTVVYEEILE
                     FPNVIATPHIAGWSRQSKLKLVEVLWNKIAKHLAGQG"
     gene            complement(4529..5305)
                     /gene="rsmA"
                     /locus_tag="PLF48_08055"
     CDS             complement(4529..5305)
                     /gene="rsmA"
                     /locus_tag="PLF48_08055"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNF49315.1"
                     /translation="MKNVTALKSYGQHFLTDTNVLNDIIQLILSQNNGLEIIEVGPGT
                     GALTKLLLDKKASLTCIELDKRCVEYLYQHFDGTKLHIIEQDVLQTDFNLLIENEAML
                     VGNFPYNISSQIVFKVLDYKEKIPVCIGMFQKEMALRLAAAPGKKDYGITSILTQLFY
                     DVKVEFDIAPQSFSPPPKVMSSVLTMKRKENVQIDFNFKLFKTIVKAGFNQRRKMLRS
                     ALKGVVNPEILKEEYFDKRAEQLSISDYIALTKRIEKENA"
     gene            5368..>5882
                     /locus_tag="PLF48_08060"
     CDS             5368..>5882
                     /locus_tag="PLF48_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49316.1"
                     /translation="MIKRADKNTKRTLLVLTTKDKVMDLPYFDEQQKKFIQSKLEKKI
                     NVFFCSKEQMTTFVYIYGYTKEGNKYEQLEHLRNDGNLVYVQLKSILEKDIFLECNID
                     LDSKQVRAFLGGLIFSDYEFLKYRPKEKKFDLTVTIPTTMLTKEEVDHLNIQKEALKI
                     TKDFVNEPVSY"
BASE COUNT         1774 a         1027 c         1042 g         2039 t
ORIGIN      
        1 ggtctttttg tgcatagatt tttatggaat tcaaaaaaat tcctatggat aacagtatta
       61 tcttgggcaa aaatagtcgc acgggtgcaa gataggaaaa aaacaaaaaa aggaatgcgg
      121 tttaaggttt attaggattt ttttaaatct ttcaaaaatt cacaaaataa acaggtgagg
      181 gcgagctcta tttgttttat cttcattctt acccgccaat cacatctccg gcgctgtctt
      241 caaagttatt tttcttttta gcaggagtag ttggtttatt gtcattttta ccaaaacggt
      301 aagtgaatgt tattgttcca atcgttgact cccagtagcg ttttgtactt cctgaaaacg
      361 tgctacctgc agtattcacg ccaaaataca tggtatgaaa aacatcgttc acatttatag
      421 caattgtgcc ttgtttgttt ttcaaaatat cttttttaaa gccaatgctg gcaaatacca
      481 tatggtttat ttttccctgt aaatagttaa acggacttct gtaatttacc atgaactgta
      541 agctggcatt ttcccaaaaa gtaaaagtgt tcataatcct gaaattgtac tgaaaagaac
      601 gcgacgaggt tgctgtggtt tccggcggca cagtgccttt catttgattg taaaacaaat
      661 tgacattcgc catgatattc caccatttga aaatgccggc acgcacagta aattctcctc
      721 cggcactctg tcctttattt aaattgtcaa aagtaacaat cgaaagtccc gtactgtcta
      781 ctaatcgata acgggtaaac atattttgtg cgtatctata ataaattgtt gatgtcagga
      841 aaacaccttt tttaaacgaa gcggtatggg taaattccaa attgtgtgaa taggccggtt
      901 tcagattcgg atttccggtt aagatattca atacatcttc gcgacttcca aaaggattga
      961 gttgttcggg ttgtggtcgg tttatccgtt tgctgtatga aattgaaatg tcattgtttt
     1021 taggcagttt caaagaaaat gaagccgacg ggaaatagtc aaaatagcgt tgtttgtttg
     1081 taattgcttg cacatattgc ttgccgttta cgaggctgta ctcgaatcgc attcctactt
     1141 tataactgaa ctttttataa tttccaccat aaatggcata agcggcattg attagttctt
     1201 tgtatctgaa tgtattggtt attgaactgt cagtttctgt tttattaagt gtatgattga
     1261 ttgagtcggc ataaaaatca ttgagcaaat tgcgatagga tgcccggtat cctgtttcaa
     1321 atttgccttt tgctttttca aagggttgcg tataatctgc ctgaaaatag gccgtatgat
     1381 ttttattttt tatcgtgttt gactgaatca aaggcacttg aacatactcg gtattattga
     1441 catcaaaata caattgtgta tagtccggat ttctgcctct gttcacataa gcatagtttc
     1501 ctgtcagcac caaatcatga gtattgcttt taaaagttcg gcggtaagaa gtagtcagct
     1561 cggcattgta atttatactt ttatccttgg tgtatctgtt gaatcctatt aagaaagcag
     1621 ctgcagagtc tgttaagtta gactgcgatt ttccattaga aaatcctaaa ttattggata
     1681 ataaggtgct gaacgaccac gtgttttttt ccttaatata ccagtcagtg gtgttggtta
     1741 gtgtattatt gatattatct gatgtcgttc tgtcttttga taacagatag taaggcgaaa
     1801 tgccatttgg aaaattaaaa cgctgactgt ttcccccata aaaagattca ttataacgaa
     1861 tggagtaatt agtggaagta gaaacctttt cattacgaaa attaagggtt acaccggtgt
     1921 taaaatcata tttcgtagca tagccgatat taaagttgcc gtttaatcct ttattggctg
     1981 actttttcaa aatgatatta atgatgccaa tatctccttc agcttcgtat tttgcagatg
     2041 gattgtttat cacttcaata ctttcaatac tattagcagg aattgcttcc agtattgctt
     2101 tagtattgcc tgctgttata cctgaaggtc tgccattaat atatattttt aaattcgtag
     2161 atcctctgtt ttccaaattg ccgtctgtgt ccacgtttaa agtaggcact tgttttagcg
     2221 catctattgc agttccgcca gctgaaagtg tattcttatc gacattgaac actttctttt
     2281 ctgcatttag tgtcatcata cttttctccg cttttatttc tacttcatca agcacttttg
     2341 catcttcatt tactttgagt gtgcctaatt ttacatctat attatcagga gggaaaactt
     2401 taagtgcagg aataatataa tctttataac ctaaaaagct gacagtcaat ttataaacac
     2461 tgtaaggaat agcgctgatt tcaaaatttc ctttttcatc aaccagtgaa cccagtatag
     2521 ttgaagtatc tttcagatgt tgaatggtaa tggaagaata atccagtggt tttccggatg
     2581 aagcatctac cacttttcct gatattctgc cagaggcagg tggttgagca aatgaaaata
     2641 aaatactgct gaatatgata aagaaaaatg atttcatgcg gcaaagataa gatttgcaag
     2701 atgttgcagt gtgttaaatc ttattacacc tatttgaaat tatttagctg ttttaagtaa
     2761 gccaactagc aaaaagctcc agcctgcaat gaaacttaat ccgcctaaag gtgttattgg
     2821 tccgataact tttagcaagg attcacttat ttgtagaata tctttacaag ctaaaatata
     2881 caatgatccg ctgaaaagaa ggataccggt acagaaaagc atggaagctg ttttaaaagc
     2941 aggaagtgca tatttttcaa acaagaaaaa tgcgataaac agtgcaaaag tatgataaaa
     3001 ctgatattgc acacctttat tccagatttc caaaccatgg tcatctacga atttgcgtag
     3061 cccatgagca ccaaatgcac ccagcattac tgcagtcatg ccgaatagcg ttgccagtat
     3121 agcgtatttt ttcatattga atgttattca gtttgttctt ccctgatttt gcggacatag
     3181 tccagtttat actgcataat atctgctata gtttgggcac gatattttgc catctcagct
     3241 ttttctcctt catataattc gtctacgcta cttttccaat gcaacagcca gtgttcaaat
     3301 tctgttttat tcattgggaa atgtttatct acctggtaat gtgcctcagt aacatttcct
     3361 cggtaaacat tgctatccaa caaaatacta tcccaaaaat ctgcaatcgt tcctaaatgt
     3421 ttgcgcaggt gttccattac cgtattttga aaaatatttt gaagtacttc atctgaaagc
     3481 aatttgccat aaaaataagc aactaaaatt tctatatcat ttcttgtggt gatatccggt
     3541 ttcatgttgt aaaaatacca acctttgagc ttaacgttgt taatttaatg ctatttatcc
     3601 ttgacctgcg aggtgtttgg ctattttatt ccagagtact tcaactaatt taagtttgga
     3661 ttgtctgctc cagcctgcaa tatgtggcgt ggcaattaca ttgggaaatt ccagtatttc
     3721 ttcataaaca acagtagggt ttatcttgtt tttagtgagt ggttcatctt caaaaacatc
     3781 aacgcaaaag ccataaattg tctgttgtct caaggccgtt aagatggctt gagtatcaca
     3841 tactgctcct cgagaggtat tgattattgt cggttttttt atgcatgttt gcaaaaatgc
     3901 tgcattgcaa taatggtgtg tttcagccga ataaggtaca tgaaagctga tgatgtctgc
     3961 ctcttgttgc aaatggtgta atgatgtttg tataatattt gtttgcttaa tttctttatt
     4021 tgggtcataa gccagtatgc taacatcaaa gcccataagc tttttggcaa atgcggcacc
     4081 ggtatgccca aaaccgataa tgcctactgt ttttccatcc agttcttcac ctctgttata
     4141 ttctctgttg aaaattccca gcttaagttc gttgttcgct attgtaatcc tgttatacaa
     4201 ggctaaaagt aatgccaaag catgttcacc aacagcattt gcattaccct ctggagaatt
     4261 aaaacaactt attcctttct ctatgcaata tgccatgtct acattttcca ttccactgcc
     4321 tactctgcca ataattttta aatgctgtgc atggtcaatg atatttttat ctattcttag
     4381 ataagttgtt attattaata atgaaaaatg gtcaatgatt aattgtaatt cttcgtatgt
     4441 aatatccggt tgaacgctta cttgccagcc ggagttttcc aactgctgtt gaagataagg
     4501 atgaatataa gccgttatca gtgcacgttt aagcattttc tttttctatt cgtttagtta
     4561 gtgcaatata gtctgaaata ctaagttgtt cggccctttt atcgaaatat tcttctttga
     4621 gaatttcagg attaactaca ccttttaatg cagaacggag catttttctt cgttgattga
     4681 atccggcttt aacgatagtc ttaaataact taaaattaaa atctatctgt acgttttctt
     4741 tacgcttcat cgtcagtacg ctgctcatca cttttggagg aggagagaag ctctgtgggg
     4801 cgatatcgaa ttcaactttt acatcataaa ataattgggt aaggatagag gtgatgccat
     4861 aatctttttt tccgggagca gcagcaagtc gtaaggccat ttctttttga aacatgccaa
     4921 tacaaacggg aatcttttct ttataatcta gtaccttaaa aacgatttgt gaggatatat
     4981 tataggggaa attgccgacc agcattgcct cattttcaat aagcagatta aaatccgttt
     5041 gtaaaacatc ttgttctata atatgcaatt ttgtcccgtc gaaatgctga tacagatatt
     5101 ccacacatct tttatccagt tcgatacacg tcagggatgc ttttttgtct aataaaagtt
     5161 ttgtcaaagc tccggttccg gggccaactt ctattatttc gagcccgtta ttttgggata
     5221 gaataagctg aataatgtca tttaatacat tagtatcagt aagaaaatgc tgtccatagg
     5281 attttaaggc tgtcacattt ttcataaaat taatacattt attatgcctg ataactctct
     5341 atctttaccc acttataaaa aaattgtatg ataaaacgag cagacaaaaa taccaaaaga
     5401 accttacttg tactaacaac taaagataaa gtaatggatt tgccttactt tgacgaacaa
     5461 caaaagaaat ttatccaaag caaacttgaa aagaaaataa acgtattctt ttgctctaaa
     5521 gaacaaatga ccacgtttgt atatatctat ggatatacaa aagaaggaaa taagtacgag
     5581 caacttgaac acttacgcaa tgatggcaat cttgtatatg ttcaattaaa aagcatactt
     5641 gaaaaagata tttttttaga gtgcaatatt gatttggaca gtaaacaggt tagagctttt
     5701 ttaggcggtt tgatattttc tgattacgag tttttgaaat atcgacctaa agaaaaaaaa
     5761 ttcgacctta ctgttacgat tccaactacc atgttgacca aggaagaagt tgaccatttg
     5821 aatattcaaa aagaggctct aaaaattacc aaagattttg taaatgaacc cgtttcttat
     5881 tt
//
LOCUS       DAOQII010000155         5869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7745_length_5869_cov_3.517888, whole genome shotgun sequence.
ACCESSION   DAOQII010000155 DAOQII010000000
VERSION     DAOQII010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7745_length_5869_cov_3.517888"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLF48_08065"
     CDS             complement(<1..390)
                     /locus_tag="PLF48_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNF49317.1"
                     /translation="MISDHPIVLFDGVCNFCNSSVQFIIKRDKAHQLRFGSLQSASGM
                     QLKKQYAIPDDLDSVILIENGKYYAKSSAALHIADYFGGGWKLLKICFLVPRFIRDFF
                     YDMIAKNRYKWFGKKETCMLPSKAQRAL"
     gene            complement(387..1631)
                     /locus_tag="PLF48_08070"
     CDS             complement(387..1631)
                     /locus_tag="PLF48_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF49318.1"
                     /translation="MRISIIIIAIGLIIFIGHYLRGVFDRKSIPDVLGLMLLGFIIGP
                     VGQLVSPDSFGKFGALFSNLVLVLILFESGIDLKLEEVKSSLKSSSGITIMGFFVTWL
                     VLTLFVKLVFNSDWLTCLYIGATMGGTSSVVVAGLVKKINILPKTSATLIMESAQTDL
                     FTLAIPISILKLMTTGEIKPEIVISGFFASLILALFIGIAGGILWLFILNKIPSMKST
                     KFSTLAFLFILYGVCEYLNYSGPLTVLTFGIILGNTKSIKLNFLKSNNQMQSAVLSKT
                     EIDFLNELVFLFRTFFFIWIGMNIKIERLDWFLMGGAATSALYLARILLIRLIADKNN
                     PVLDNAAISIMIPKGLGAAVIATLPAQAGLADGTFIQSFCYSVIFFSTLLCVLFFFLI
                     KSGISMPIYNLIFSNKYNTKPS"
     gene            complement(1725..3620)
                     /locus_tag="PLF48_08075"
     CDS             complement(1725..3620)
                     /locus_tag="PLF48_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49319.1"
                     /translation="MKFYLTLIILCFFSHIGLAQYEKTGALPANPYLNQQTNADFLKQ
                     KHAYRISALALPFFDDFNQLEVHPNPLFWQDNNVYVNKHYPQHTVTLGVATFDGTDSV
                     GNPYSLTPDARGIADILTSNSIDLSGLTRDSLVFLSFFYANGSYGEEPESLQDNFLVQ
                     FLDGSGNWTDQWTVTPDGIHTPRQVFIKVDSAFLHSNFQMRFISVGSLTGANDAWHLD
                     YVRLDKNRDTSVDKNIREMAYEFAPSSLLKPYYSMPYSHFDSTFLKDTVAVYVKNSFI
                     NPTTDIKDHFTATVVNDGAVIANYDGPSRDFLPDTENEIKYPKFSIPTGLSYDTVVVS
                     VNYNFECSAEAGAPAAVLANNQVTYNQVFSNYFSYDDATPERGYWIDLNNAYIGVKYK
                     LSHPDTLQAIKLQAFPTRVNNNLGLFSICVWKKLTRNDYYNPSDLIYEQPLLRLSNLN
                     KEFGVDTLNNFLYAAIKPDFVKNGATFPLVLSDSFVVGIMVVDKETLSLGFDRNTNSS
                     TKTFYTDINEKWRESVISGSVIINPVMGKQLPDYLTPVIQHASNLQDIKIYPNPVSDF
                     LTLSKLQEPGRIEIYAINGALMSTVNVSSENVRLDMSALPSGSYYVRLTEEISKASAT
                     YKIQVLH"
     gene            complement(3659..4399)
                     /locus_tag="PLF48_08080"
     CDS             complement(3659..4399)
                     /locus_tag="PLF48_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNF49320.1"
                     /translation="MAKLIGAILSSLGISIALLFLIIFSLNAYTKHGQEQEVPNVKGK
                     SFYDAKKILSKSDLDYIVVDSSYTENLPPLAIIDQQPRLGNKVKKGRKIYLTVNTSIP
                     PSVKIPNLIDVSKRQAEIILSSWGLKVGQYMYVPDMALDAVQNMQIKGRDVKAGEVIP
                     KGTAIDLVLGDGFGNQMTEVPPLTDLTVIEAKEVLKAVQLNLGNIIAEGKITDTSSAY
                     VFDQDPKYGVPGKLGDNNSVTLYIRQEK"
     gene            complement(4549..4959)
                     /locus_tag="PLF48_08085"
     CDS             complement(4549..4959)
                     /locus_tag="PLF48_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49321.1"
                     /translation="MKRVYKILFIVLLQMFFIEQCVTADSLDVTPSKGKSTVRSIWKY
                     EQDFSKYNAEDGIAKKIFYLCLNDDGTYNQYVMVVPEKNYPYMKVQNYTLDGTWEEKN
                     GQLILEENGKKNAIAYTYFVSNFENIDNQIIYTK"
     gene            complement(5294..>5869)
                     /locus_tag="PLF48_08090"
     CDS             complement(5294..>5869)
                     /locus_tag="PLF48_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF49322.1"
                     /translation="EFAGLDIIKAKELAQRGHDIFFSVEHMPIPVIAVVNGFALGGGC
                     ELSMACHMRIATQNAKFGQPEVNLGIIAGYGGTQRLTQYIGKAKSIELHLTADMIDAQ
                     TALNLGLVNYVEPDKNAALDKAKSIIQKIATKGPVAVAKAIECINAYYKDGVDGFAYE
                     VSAFGDLFNTTDVKEGVDAFLNKRKPEFKGE"
BASE COUNT         1962 a         1082 c          974 g         1851 t
ORIGIN      
        1 taatgctcgt tgcgctttgg aaggaagcat acaagtttct tttttaccaa accatttgta
       61 tcggttttta gcaatcatat catagaaaaa atctcttata aaacgaggca cgagaaagca
      121 aatttttaac aacttccatc ctcctccgaa gtaatcagca atgtgtaatg ctgccgaaga
      181 ctttgcataa tattttccgt tttctatcaa gattacacta tccaaatcat ccggaattgc
      241 atattgcttt ttcaattgca tacctgatgc agactgcaag ctaccgaagc gcagttgatg
      301 tgctttatct cttttgatta taaactgaac acttgaattg caaaaattgc aaactccgtc
      361 gaataagaca atcgggtgat ctgaaatcat gatggcttag tattatactt attactaaaa
      421 atcaggttat aaataggcat actgattccg gatttaataa gaaaaaagaa gagtacacag
      481 agcaatgtac taaaaaaaat aacggagtaa caaaacgatt ggatgaatgt accgtcagcc
      541 aatcctgcct gagcgggcaa tgtggcaatt acagcagcgc ccaaaccttt tggaatcata
      601 atggaaattg cagcattatc taataccgga ttatttttgt cagctataag tctgattaac
      661 aagatacggg ctaaatacaa ggcagaagtt gccgctccgc ccatcaaaaa ccaatcgagt
      721 ctttcaattt ttatgttcat gccaatccaa ataaaaaaaa atgttctgaa taagaatacg
      781 agttcattta aaaaatcaat ttccgtttta ctaagtacag cagactgcat ttgattattc
      841 gattttaaaa aattgagttt aatgctttta gtgttcccca atatgatacc aaacgtcaaa
      901 acagtcaatg gtccgctata attaagatat tcgcatacac catataaaat aaacaaaaag
      961 gccagcgtgg aaaattttgt agatttcatg gaaggtattt tgttcaaaat aaacaaccat
     1021 aaaattccac cggctatgcc aataaagaga gctagtatca atgatgcaaa aaaaccggaa
     1081 atcactattt caggttttat ttctccggtg gtcatcagct tgagtataga tatgggaatg
     1141 gctaaagtaa ataaatcagt ttgtgcagac tccataataa gtgttgccga agttttaggt
     1201 aggatattta ttttttttac caaccctgcc actactacag aagatgttcc gcccatggta
     1261 gctccaatat aaagacaagt gagccaatcg ctgttaaaga ctaattttac aaataaagtt
     1321 aaaactaacc atgtaacgaa aaaacccatg atggtaatac ctgaagatga tttcaaagaa
     1381 gattttactt cttccaattt taagtctatt ccactttcaa ataaaattaa aacaagaacc
     1441 aaattgctga ataatgcacc gaattttcca aaagaatccg gactgaccag ttgaccgact
     1501 ggtccaatta taaaacccaa taacattaat cctaatacat ctggaatact ttttctgtcg
     1561 aatacacctc gcaggtaatg tccgataaaa ataatcaaac ctatcgctat gataataatc
     1621 gatatgcgca tcttagccgt attctaccat gaaaataata aaaaattagg caatattact
     1681 caataaaatt tcagataagc agtaacgaaa atgagaaatt gcaattaatg caaaacctga
     1741 atcttgtagg tagctgaagc ttttgatatc tcttcggtta atctgacata gtaacttcct
     1801 gatggcaaag cagacatatc cagtcgcaca ttttctgaag acacattaac agtactcatt
     1861 aatgcaccat taatggcata aatttcaata cgccccggct cttgcaactt agacaaagta
     1921 agaaaatctg aaacgggatt tggataaatt ttaatatcct gcaaattaga agcatgctga
     1981 ataacaggag ttaaataatc aggcagttgt tttcccatta ccggattgat tattacagaa
     2041 ccggatatta cagactcacg ccatttctca ttaatatctg tataaaaggt ttttgtagag
     2101 ctgtttgtgt ttctgtcaaa gcccaatgag agggtttctt tatcgacaac cataataccg
     2161 accacaaatg agtcggataa aacaagtgga aatgtagctc cgttttttac aaaatcaggt
     2221 tttatcgctg cgtataaaaa attattaagg gtatcaacac caaattcttt attcaggttt
     2281 gacagtctta ataagggctg ttcgtatatt aaatccgacg ggttgtaata atcattacgg
     2341 gtaagttttt tccagacaca tatactgaat aagcccaggt tattattcac tcgggtagga
     2401 aaagcttgta gtttaatagc ctgtaaagta tctggatgac tcagcttata tttcactcca
     2461 atataagcat tattaagatc tatccaatag cctctctcag gcgttgcatc atcatatgaa
     2521 aaataattgc tgaaaacttg attataagtt acttgattat tggctaaaac tgctgccgga
     2581 gcgccggctt ctgccgaaca ttcaaaatta taatttacag aaacgactac cgtgtcatag
     2641 gataatccgg ttggaattga aaatttcgga tatttgattt cattttccgt atcaggtaaa
     2701 aagtcgcgtg aaggaccatc atagtttgct attacagcac catcattaac cactgtagcc
     2761 gtaaaatgat ctttaatatc agttgtcggg ttgataaagc tgtttttaac ataaacagca
     2821 accgtatctt ttaaaaatgt agaatcgaaa tgtgaatagg gcatagaata ataaggcttc
     2881 aataagctgg aaggagcaaa ttcataggcc atttctctta tatttttatc tacagaagta
     2941 tctctgtttt tatccaagcg cacataatcc aaatgccagg catcatttgc tccggttaaa
     3001 ctaccgacag atataaagcg catttgaaaa ttactgtgta aaaatgcaga atctactttt
     3061 ataaatactt gtcttggcgt atgaatgcca tccggtgtaa ctgtccattg atctgtccaa
     3121 ttgccacttc cgtccaaaaa ttgaactaaa aaattatcct gtaatgattc aggttcttcg
     3181 ccatacgacc cgttagcata aaaaaaactc agaaatacca gactatcccg ggttaatcca
     3241 cttaaatcta ttgaattaga agttaaaata tctgcaattc ctcgagcatc tggtgttaaa
     3301 ctatatggat taccaacaga atctgttccg tcaaaagtgg caacacccaa ggttacggta
     3361 tgctgaggat aatgtttatt gacatatacg ttattgtctt gccaaaaaag aggattcggg
     3421 tgaacttcca gttgattgaa atcgtcaaaa aatggcagtg ccaaagcaga aatacgatag
     3481 gcatgttttt gtttcaaaaa atctgcattt gtttgctgat taagataagg gttagccggc
     3541 aaagcacccg tcttttcata ctgagccaag ccgatatgag aaaaaaagca gagtattatg
     3601 agcgtcaggt aaaatttcat gctattatta agttgtaaaa aaacgtcttt tggttgtttt
     3661 atttttcttg tctgatataa agagttacgg agttattatc ccctaatttg ccgggcacac
     3721 catatttcgg atcttgatcg aatacatacg cacttgatgt atccgtaatt tttccttcag
     3781 caataatatt tccaagatta agctgaactg cttttagcac ttcttttgct tcgataacag
     3841 ttaaatctgt aagcggtggt acttcagtca tttgatttcc aaacccatcg cccaaaacca
     3901 gatctattgc agtacccttt ggtattacct cccctgcttt gacatctctg ccttttattt
     3961 gcatattttg gacagcatcc aatgccatat caggcacata catatactgt cccactttca
     4021 agccccagct agacaggata atttctgctt gtcttttgga tacgtctatt aaattgggaa
     4081 tttttacgct gggtggaatg ctcgtattta ccgttaaata aatttttctg ccttttttta
     4141 ctttatttcc cagacgcggt tgttggtcga tgatggccaa aggtggtaaa ttttcggtat
     4201 acgatgaatc aactacgata taatccaaat cactctttga caatattttt ttagcatcat
     4261 aaaatgattt tcctttaaca ttggggactt cttgttcttg tccatgttta gtataagcat
     4321 tcaaagaaaa tataataaga aagagaagag caatgctaat tcccaaagaa gataaaatcg
     4381 cgccgattaa ttttgccata cagtagtgct tcgtggttca aatatattta attttgggta
     4441 aaacagcgag taaaaattta ttaaaaaagg ctaaaacaga cagaaaaaaa ttaagccacg
     4501 tgtataacta caggcggctt agtgtaaaca acaaaaattt aatgagaatt atttcgtata
     4561 tataatttga ttgtcaatat tttcaaaatt ggatacgaag taggtataag ctattgcatt
     4621 ttttttaccg ttttcttcta gtatcaattg tccgtttttt tcttcccaag ttccatccaa
     4681 ggtataattc tgaactttca tataggggta gtttttttcc ggtactacca tcacatattg
     4741 attatacgtt ccatcatcat ttaaacagag ataaaatatt ttcttagcaa taccatcttc
     4801 tgcattatat tttgagaaat cttgctcgta tttccatata cttctgactg tagatttgcc
     4861 tttgcttggc gtaacatcca aactgtcagc agttacacat tgttcaataa agaacatttg
     4921 caacagcaca ataaacaata tcttatatac ccgtttcata atctagctaa gtttctgtta
     4981 tatggttttt gatgaggatt ttagttctaa actttagagc cttagccgta aacaacaaat
     5041 tgagagaaga ttgtaagaac tctttatgat acaatattag atagtttttg atttacatca
     5101 ataaaaactt agtaaacggt aggttttacc acataagtgg cagaaatgaa ctaataaatg
     5161 gaaaaatttg acttcaaacg tcaaaattgc acatgaagat ataatttaaa tttatgttaa
     5221 aataaaataa aaatattaac atatttgcat gtaaacaaaa taaaaaagac caaaaaaact
     5281 gaggtaaaca gggctattca cccttaaatt ccggtttgcg tttgttcaaa aaagcatcta
     5341 ctccttcttt gacatcggtc gtattgaata aatcgccaaa tgcactgact tcataagcaa
     5401 agccgtctac accatcttta taatatgcat tgatacattc tatagcttta gccacggcta
     5461 caggtccttt agtggctatt ttttgaatga tagattttgc tttatccaaa gcagcatttt
     5521 tatccggttc tacataattg actaagccca ggtttaatgc agtctgtgca tctatcatat
     5581 ctgctgtaag gtgcagttca atagatttag ctttgccgat atattgtgta agccgttgcg
     5641 taccgccata tcctgctatt atgcccaaat taacttccgg ctgtccgaat tttgcatttt
     5701 gagtcgcaat ccgcatatgg caagccatac tcagttcgca acctccgcct aaggcaaaac
     5761 catttacaac agcaattacc ggaatgggca tatgttctac agaaaagaaa atatcatgac
     5821 cacgttgtgc cagttctttg gcttttataa tatccaaccc tgcaaattc
//
LOCUS       DAOQII010000156         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7888_length_5810_cov_4.582971, whole genome shotgun sequence.
ACCESSION   DAOQII010000156 DAOQII010000000
VERSION     DAOQII010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7888_length_5810_cov_4.582971"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..426
                     /locus_tag="PLF48_08095"
     CDS             235..426
                     /locus_tag="PLF48_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49323.1"
                     /translation="MKKMIFICMAAGFIATACNPDWFKKEPKNEEKEVVIPDDSVSDS
                     TLLSEDTSQVKTDSVQLKH"
     gene            569..946
                     /locus_tag="PLF48_08100"
     CDS             569..946
                     /locus_tag="PLF48_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF49324.1"
                     /translation="MQTHAISWFEIPVLDFPRAKKFYNTILNDEIHEMLMGEQLMGFF
                     PCNPADGGVGGALVLADGYEPSKKGTKVYLNAGDNLYTVLNRVEAAGGKIITDKTEIT
                     PEYGYYAEFEDTEGNYISLHSKG"
     gene            complement(1017..1553)
                     /locus_tag="PLF48_08105"
     CDS             complement(1017..1553)
                     /locus_tag="PLF48_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49325.1"
                     /translation="MKWIIFLGLIGYYLYKAYLEAAKKQAQQGNLPEKSGRNDSPSPK
                     KQKSFEDILKDLEDALEGKTTPPQPQTTAKQEFKPYAQQQKRTIYESKQDEDSIENET
                     MYMESVEDGTVDKYSNLGNYKSDISSIETMETRIEAVDVEGFEKQQPKPPKFMILNKK
                     KMNAKYLFTAQIILERKF"
     gene            1574..5182
                     /gene="smc"
                     /locus_tag="PLF48_08110"
     CDS             1574..5182
                     /gene="smc"
                     /locus_tag="PLF48_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNF49326.1"
                     /translation="MSINKQLTGKIRSAQILFLQAMRLRSLDIKGFKSFAEKTQINFE
                     KNITGIVGPNGCGKSNVVDAIRWVLGEQKTKQLRLEKMDNIIFNGSKDKAASNIAEVS
                     LTLDNTRNLLPTEFQSVTITRIVEREGDSEYKLNGVTCRLKDIRDLFMDTGISTDSYA
                     IIELKMIDQILNNVDNARRTLIEQAAGVSKYKTRKKETLNKLSATEADLNRVEDLLFE
                     IDKNLKSLETQAKKARQYKNYKEEYKIISIDLSKYELHGINESFIASNKEITEQEDLR
                     LEVNTQIEVLESGLEKEKLLVLEKEKSLSEHQKLLNHFLAEIQESESTKKMDEQKSAF
                     LRDKINQLNKQIEQTKSIITSLVTESESLEKYIADDSEKLEVVRLDFETAKQKLEDQR
                     NTNLDFRTKLDEARQKHQASAYQITNTEKQIAVKESQHENLMKRAEQSLFENEERLRT
                     LESLRNEIKQSETSVEKHKLLVDDLRKKEEENLEKIKNLEEKNEQLRQELTNKNRTLD
                     AKNNEYKLTKNMIDNLEGFPESIKFLKKNVSWLKDTPLLSDIIYTEEKYRIAIESVLQ
                     PYLNNFIVEKENEALNAIDILSQSSAGKAGFLILDYFKSQVAVKQHSAIEHAVTANDV
                     IQIEDKYKPLLTYLLQGIFIANEGADVNKLFQQLPDKKDVYIISNNGHLLKSDKQISG
                     GAVGLFEGKRLGRTKNLEILEKDIKKLETEVLELKKVTQDTHNELQTLKVNSYRNVIE
                     REDKELQNQEKQLVALHSKLESEENAVGKVNTQAQELRDAINALLEEINPLKENLAHH
                     IQTADADKNSMDVMELDFRKANEVYNQISQEYNQSNILFIQQENKLKSNQQTLQYKQS
                     QLEENNKRLETADMDITTAQAEISILQKNIAEAEELLVMSYKEKDDKMQTLSAQETDY
                     YKLKDEISNSEETIREKSKYKTQIEERIAGKKDLVSDLKLRLNVLKERLSIEFHINPD
                     EFTENLELPETPKDELQDRIDKVKRRIENFGEVNPMAEEAYNEMKDRYDFINTQKQDL
                     LDSKANLLETMLEIENTAKGQFMETFNAVRENFIEVFRSMFTEDDNCDLILVNPDDPL
                     ESEIDIIAKPKGKRPQSINQLSGGEKTLTALSLLFGLYLYKPAPFCILDEVDAPLDDT
                     NIKKFNDAIRKFSQNSQFIIVTHNKSTMASVDNIYGVTMVKQGISRVVPVDFSNLN"
     gene            5204..5611
                     /gene="mce"
                     /locus_tag="PLF48_08115"
     CDS             5204..5611
                     /gene="mce"
                     /locus_tag="PLF48_08115"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNF49327.1"
                     /translation="MKKIDHIGIAVNDLADSTALFNKLFNRTPFHIEILEEMHLEAAF
                     YQLDDTKVELLHPQNEQSTVHKYLENKGQGIHHVAFEVEDIYAEINRLKKEGFQPLTE
                     QPYKGALNKLVCFFHPKTTNGVLIELCQKIKDE"
     gene            complement(5626..>5810)
                     /locus_tag="PLF48_08120"
     CDS             complement(5626..>5810)
                     /locus_tag="PLF48_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49328.1"
                     /translation="QSGYCILENKRVAVINKFYDTEARINALVEIMQQIELTSDELDE
                     KQMEIFNKVAQHKLSL"
BASE COUNT         2088 a          972 c         1102 g         1648 t
ORIGIN      
        1 taatctattt acatattcta cattcacaaa atttaaaatt atataattat ttaagtataa
       61 atgtttttac gcattaataa taggtctgtt tttttaattt gtagtattgt gaagtattga
      121 taacatgaat tgcaattatc tttattttta ctggttaaaa tatcttaaaa ttccaggttg
      181 tagaaactat ttgcaacaga atagcgtatt aacaataacc aaatcaccaa ccaaatgaaa
      241 aaaatgattt tcatctgcat ggctgccggc tttatcgcaa cagcatgcaa cccggactgg
      301 ttcaaaaaag aaccaaaaaa tgaagaaaaa gaagtcgtta ttcctgatga ctcagtttca
      361 gactccacct tgttaagtga agacacgagt caggtaaaaa ccgacagtgt acaattgaaa
      421 cactaacatg cagtaaaagg ttggggcggc gcttcgcgcc gccccaactt attttttaag
      481 tctttaagcg ctgtaaaagt tattcgattt taaatttgca ttcaatttta ttctgaaatt
      541 actaaattta cttacaaaat aattaattat gcaaacacat gccatcagct ggttcgaaat
      601 tccggtattg gattttccca gagctaaaaa attttacaat acaatcttaa atgacgaaat
      661 ccacgaaatg ctgatgggtg agcaacttat gggctttttc ccttgcaatc ctgctgatgg
      721 tggagtagga ggcgctctcg tattggcaga tggatatgag ccatcaaaaa aaggaacaaa
      781 agtttatcta aacgcaggag ataatctata taccgtgcta aacagagtag aggctgccgg
      841 aggaaaaatt ataactgata agactgaaat tactcctgaa tatggctatt atgccgaatt
      901 tgaggataca gaaggtaatt atatcagcct gcactctaaa ggttgatact cgattaccgt
      961 acaacttgta tcttgtatat tgaatgctga agtttttgca aaatgaagaa gtatgattaa
     1021 aacttccttt ccaaaataat ttgtgccgta aacaagtatt tggcgttcat ttttttcttg
     1081 tttaaaatca tgaatttagg cggtttgggt tgttgttttt caaaaccttc tacatctaca
     1141 gcttcaattc tcgtttccat ggtttcaata gaactgatat cgcttttata attgcctaaa
     1201 ttagaatatt tatcaaccgt accatcttcc acactttcca tgtacatcgt ttcattttca
     1261 atgctgtctt catcttgttt tgactcgtaa attgtacgct tttgctgttg agcataaggc
     1321 ttaaattctt gtttagcagt agtttgtggc tggggaggag ttgtttttcc ttccaatgca
     1381 tcttctaaat cctttaaaat atcttcaaaa ctcttttgct tttttggcga tgggctgtca
     1441 tttcttcctg atttttcagg caaattaccc tgctgagcct gcttttttgc tgcttcaagg
     1501 taggctttgt ataaataata gccaattaga cccagaaata ttatccactt catacgttca
     1561 aaaataccaa aaattgtcaa taaataaaca attaacaggt aaaatacgtt ccgcacaaat
     1621 cttatttttg caggctatgc gattgagaag tctggatata aaagggttta agagttttgc
     1681 ggaaaagacg cagattaact ttgaaaaaaa cattacgggc attgttggcc ctaatggttg
     1741 cggcaagtcg aatgtagtgg atgccattcg ttgggtattg ggcgagcaaa aaaccaaaca
     1801 acttcgtttg gagaaaatgg acaatatcat cttcaacggc tctaaagata aagccgcttc
     1861 taacattgcc gaagtgtcac taactttgga taatacccgc aatttgttac ctacggagtt
     1921 tcagagtgta accattaccc gtattgtaga gcgcgaaggc gacagtgagt ataaactgaa
     1981 tggggttaca tgtcgtctga aagatatccg cgatttattt atggataccg gaatttctac
     2041 cgattcttat gcaatcatcg agttgaagat gatagaccag attctgaaca acgtagataa
     2101 tgcccgccga acattgatag aacaggctgc aggcgtttct aaatataaaa ccagaaaaaa
     2161 agaaacttta aataaactga gtgccacaga agccgacttg aaccgtgtgg aggacttatt
     2221 gtttgaaata gataagaatc tgaaatctct tgaaacacaa gcaaaaaaag ctcgacaata
     2281 taaaaactac aaagaagagt ataaaatcat ttctattgat ttatcaaaat atgagttgca
     2341 cggtattaat gaaagcttta ttgcctcaaa taaagaaata acggagcagg aggatttgcg
     2401 cttagaagtc aatacgcaaa ttgaagtatt ggagtcaggc ttggagaaag aaaaactgct
     2461 ggttttagaa aaagaaaaat cgctgtcaga acaccagaag ctactgaatc attttttagc
     2521 ggaaattcag gaaagtgaga gtactaaaaa aatggatgaa cagaaatctg cttttttacg
     2581 ggataaaatc aaccagttaa ataaacaaat tgaacaaacg aaatcaatta ttacttcctt
     2641 agttaccgag tctgaaagcc tggaaaaata tattgctgat gacagtgaaa aactggaggt
     2701 ggtgagattg gattttgaaa cagcaaaaca aaaattagaa gatcaacgta atactaatct
     2761 cgatttcaga actaagctgg atgaggcacg tcagaagcat caggcatctg cttatcagat
     2821 taccaatacc gaaaaacaga ttgccgtaaa agaatctcaa catgaaaatc tgatgaaacg
     2881 tgcagagcaa tctttatttg aaaatgaaga aagactgcga accttagaat ctttgcgcaa
     2941 cgaaattaag caatcagaaa cttcggtaga aaaacacaaa ttactggttg atgatttgcg
     3001 caaaaaagaa gaagaaaatc tggagaaaat taaaaatctg gaagagaaaa acgaacaact
     3061 tcgacaagaa ttaaccaata aaaacagaac actcgacgca aaaaataacg aatacaagct
     3121 taccaaaaac atgatagaca atctggaagg ttttccggag tctatcaaat ttttgaaaaa
     3181 aaatgtaagc tggttgaaag acactccttt attgtctgat attatttata ccgaagaaaa
     3241 ataccgcatt gctattgaaa gtgttttaca accttatttg aacaatttta tagtagaaaa
     3301 agaaaatgag gcattgaatg ccattgatat tttatcccaa agctcggcag gaaaagccgg
     3361 atttttgatc ttggactatt tcaaatcaca agttgctgtt aaacaacaca gtgcaattga
     3421 acatgccgta acagccaacg atgttatcca gattgaggat aaatacaaac ctttattgac
     3481 ttatttgttg caggggattt ttatagcgaa tgaaggtgcg gatgtcaata aattatttca
     3541 acaattaccg gataaaaaag atgtgtatat catctccaat aacgggcatt tactgaaatc
     3601 cgacaaacaa atttcaggcg gtgcagtggg tttgtttgaa gggaaaagac tgggaagaac
     3661 caaaaatctg gagatcctgg aaaaagatat caaaaaattg gaaactgaag tactggagct
     3721 caaaaaagta acccaggaca cacataatga gttgcaaacg ctgaaagtaa attcatacag
     3781 aaatgtaatt gaacgcgagg ataaagaact gcaaaatcag gaaaaacaat tagtggctct
     3841 tcattctaaa ttggaaagtg aagaaaatgc ggtaggaaaa gtaaacaccc aagctcagga
     3901 acttcgtgat gccatcaatg cattactgga agaaataaac ccgttgaaag aaaaccttgc
     3961 ccatcatatt caaacggcag atgcggataa aaactcgatg gatgtcatgg aactggattt
     4021 ccgcaaagct aatgaagtgt ataatcagat ttctcaggaa tataatcagt cgaatatttt
     4081 atttattcag caagaaaata aactaaaatc taaccaacag accctgcaat acaaacagtc
     4141 gcaactggaa gaaaataaca agcgtttgga aactgcagat atggacatca ccacagctca
     4201 agctgagatt agtattttac aaaaaaatat tgccgaagct gaagaattat tagtgatgtc
     4261 ttataaagag aaagacgata aaatgcaaac actttctgct caggaaactg actattataa
     4321 actgaaagat gaaatcagca attccgaaga aactatcagg gaaaaatcaa aatataaaac
     4381 acagatagaa gaacgcatcg ctggaaaaaa agatttagtc agtgatttaa aattgcgttt
     4441 gaatgtatta aaagaaagac tttcgataga gtttcacatc aatcctgatg aatttaccga
     4501 aaatctggaa ttgccggaaa cgcctaaaga tgaattgcaa gaccgaatag ataaggtgaa
     4561 acgccgtatt gaaaatttcg gtgaagtaaa cccgatggca gaagaagcct acaatgaaat
     4621 gaaagatcgc tacgatttca tcaatacgca gaagcaggat ttgttagact cgaaagcgaa
     4681 cttacttgaa acgatgctgg aaatagagaa cacggcgaaa gggcagttta tggaaacctt
     4741 taatgccgtg cgtgaaaact ttattgaagt tttccgcagc atgtttaccg aagatgataa
     4801 ttgtgatttg attttggtaa atcctgacga cccattggaa agtgaaatcg atattattgc
     4861 taagccaaaa ggtaagcgtc cgcaaagtat caatcagttg tcgggaggag agaagacctt
     4921 gacggccttg tcattattgt ttggattgta tttatacaaa ccggctccgt tctgtatttt
     4981 ggatgaggta gatgcaccgc tggatgatac gaatattaaa aaatttaacg atgctatccg
     5041 caaattttca caaaattcgc agtttatcat cgtaacgcac aacaaatcta cgatggcaag
     5101 cgtggataat atttatggcg ttaccatggt aaaacaaggt atttcgcgcg ttgtgcctgt
     5161 cgatttcagt aatttgaact agtttttaaa ttgttttttc cttatgaaaa aaatcgacca
     5221 tattggcatt gcagtaaatg atttagccga cagcactgca ttatttaata aactgtttaa
     5281 ccgcacacct tttcatattg aaatacttga agagatgcat ttggaagcgg cattttacca
     5341 actggatgat accaaagtag aactattgca tccgcagaat gaacaaagca ccgtacataa
     5401 atatttagaa aataaagggc agggcatcca tcatgtagcc tttgaggtag aggatattta
     5461 tgctgaaata aaccggctta aaaaagaagg atttcagcca ttaacagaac aaccttacaa
     5521 aggagcatta aataaattag tatgtttttt tcatcccaaa actactaatg gcgtactgat
     5581 tgaattatgt cagaaaataa aagatgagta gtagagcaac taaatttata aactcaattt
     5641 gtgctgtgcc actttattga aaatttccat ttgcttttca tcaagctcat cactggtgag
     5701 ttctatttgt tgcatgattt ccaccaaggc atttatgcga gcttcggtat cataaaattt
     5761 attgatcacc gccacacgtt tgttttctaa tatacaatag cccgactgaa
//
LOCUS       DAOQII010000157         5792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7928_length_5792_cov_3.511417, whole genome shotgun sequence.
ACCESSION   DAOQII010000157 DAOQII010000000
VERSION     DAOQII010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7928_length_5792_cov_3.511417"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLF48_08125"
     CDS             complement(<1..849)
                     /locus_tag="PLF48_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49329.1"
                     /translation="MKKIIINSLIAIFTIFIVNELIKARSAGIAGVTSSPGENNCHNC
                     HGDYTTNSGTGSVTISTTIPAAGYTLGQTYSVSVTVARTASTLFGFDCEALTTTNTNA
                     GTLAITDAASTKLMTSSGKTNVVHTKNGGLVSGSKTFTFSWTAPATNVGTVTFYVSGL
                     CCNNNGNESGDYVYTKSAAFSMAVATATVTTGTISGSPFCQGQTGISVPYTVSGTFNT
                     GNVFTAQLSDASGSFTTPNTLGTLTSTTAGTIVTTIGLPAVAGTGYRIRVISSNPAVT
                     GTDNGTN"
     gene            complement(871..1521)
                     /locus_tag="PLF48_08130"
     CDS             complement(871..1521)
                     /locus_tag="PLF48_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49330.1"
                     /translation="MSKSLLAVFFFVMLLFAQCKHEPEKITITNPPTNAKDSICFEEE
                     VLPMLASNCAMSGCHDADAANAGVVLDSYDHLKATISGSLLLQSVKDTGAFRMPLPPA
                     PALSSTQISVLENWVNQGMKIGIDCEGPCDTSNVTYSGVILPLLQNSCISCHSGNYEP
                     DLTNYNQVKSQVNNGKLSGSVNHLPGYDAMPYNQAKLSDCKLAQIRIWIAAGAPNN"
     gene            complement(1644..3779)
                     /locus_tag="PLF48_08135"
     CDS             complement(1644..3779)
                     /locus_tag="PLF48_08135"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF49331.1"
                     /translation="MDIYEIAKDFILNTAESVFLTGKAGTGKTTLLKYIQQHTQKNCV
                     IAAPTGVAAINAGGVTLHSLFQLPLNMYVPVKNRNYAVNNPATLFEHVRLNRQKIQLL
                     REMELLVIDEISMCRCDILDEIDLLLRSFRRRMNEPFGGVQVLFIGDLYQLPPVVKDE
                     EWRILNEFYESPFFFSAWVMKEFQVLHIELETVFRQTDEEFIDLLNAVRNNAVNKSDL
                     DELNKRYNPHAAQNAENTILLTTHNRKADKINQEELSKLASEIHTFEANIHGDFLESS
                     YPADKELMLKVGAQVMFTKNDLTQEKRYYNGKIGTVTRLNEEEIVIKCVEDNYEINVD
                     SHTWENLRYAYNDTTDKIDEDVIGTFEQYPLKLAWAVTIHKSQGLTFDKVIIDAGHAF
                     AAGQVYVALSRCRSLEGISLLTPVQADAIFTDDRILQYSADKSNPDALASLLPLAKLE
                     YSKKKLLTSFSWRKITDHILQFQGIIHEKQIPEKAEAMELAIQMEKSVRNLEEVSAKF
                     LIQLKDILNQVEDEKYRILLNERVIKSIQYFCSKINDELIKPLEAHIEKMRIKNKTKG
                     YVRQAKNLATVFVDKLQEIERLKFGDSLFYTGTSFYQEIQHETVQLKNTTEKGSTFRD
                     TLKLYQEGKSIEEIAGIRALAVSTIESHLLKWIQLGDLEAKEFISEADMDIIQAYVKN
                     NTVNSLTELKNHFEDEYSFFQLRICLNTN"
     gene            3859..4245
                     /locus_tag="PLF48_08140"
     CDS             3859..4245
                     /locus_tag="PLF48_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNF49332.1"
                     /translation="MDAFILQVPGRIQEILPILLMFIVAMGFVVLVMVLTHWMGPKRN
                     TKTKLESFECGIEQHGNARTPFSVKYFLVAVLFVLLDVEVIFMYPWAVNFRKLGILGI
                     TEMFTFIFIFLVGFYYILKKGALKWE"
     gene            4315..4866
                     /locus_tag="PLF48_08145"
     CDS             4315..4866
                     /locus_tag="PLF48_08145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNF49333.1"
                     /translation="MSSVIDIKEEYKPIGLEGQGFFATQLDKVVGLARKNSLWPLPFA
                     TSCCGIEFMATMAAHYDLGRFGSERLSFSPRQADMLMVMGTISKKLAPVLRQVYQQMA
                     EPKWVLCMGACACSGGVFDTYSVLQGIDNIIPVDVYIPGCPPRPEQVLDGILQIQKLA
                     ESEARNRRESPEYKAMLQRYGLE"
     gene            4882..5388
                     /locus_tag="PLF48_08150"
     CDS             4882..5388
                     /locus_tag="PLF48_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNF49334.1"
                     /translation="MQILTTFIESTLNEQFPGALLQIEEQYDFPVFIIANDQVKNVLK
                     FLKENKDTDFHFLTTLCCIHYNLNTADKEFAMMYQLHNMPKGWRIRIKTFLPKDELTV
                     PTVTDLWPAANWLERQEFDFFGIFFDGHPNLKRILNMDEMNYHPMRKEYALEDFGRTD
                     KDDKMFGR"
     gene            5478..>5792
                     /locus_tag="PLF48_08155"
     CDS             5478..>5792
                     /locus_tag="PLF48_08155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611072.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNF49335.1"
                     /translation="MSTPIYNHEEKQYTTLNLGPTHPATHGIFQNVLTMDGEIIVHAE
                     PTVGYIHRAFEKLAEHRPYNQITTLTDRLNYCSSPINNMGWWLTVEKLYGIDIPKRAE
                     YLR"
BASE COUNT         1716 a         1066 c         1040 g         1970 t
ORIGIN      
        1 atttgtacca ttatctgtac ctgttacagc agggttactg gatattactc ttattctgta
       61 tccagttccg gctacggccg gcagtcctat agtagttaca attgttccgg cagttgttga
      121 tgttagagtt cctagtgtat taggtgtagt aaatgaacca ctcgcatcgg ataattgtgc
      181 ggtaaaaaca tttccggtat taaaagttcc cgaaacagta tatggaacac ttattcctgt
      241 ttgtccttga cagaaaggac taccactaat cgtgccggta gtaacagtag ctgttgctac
      301 agccatacta aatgccgcag attttgtata aacataatct ccactctcat ttccattatt
      361 attacaacac aaacctgaaa catagaaagt aactgtacca acattggtag caggtgccgt
      421 ccaactgaat gtaaatgttt ttgaacctga aactaaacca ccgtttttgg tatgcactac
      481 attggtcttt ccgctggaag tcattaattt tgtagaagct gcatctgtaa tagctaatgt
      541 tcccgcattt gtatttgtag tagttaaagc ttcgcaatca aaaccaaaga gtgttgaggc
      601 tgtccgggca actgttacac taactgaata cgtttgccct aaggtatatc ctgctgccgg
      661 tattgttgta gaaatagtta cacttcctgt gcctgaattt gtagtataat ctccatgaca
      721 attgtgacaa ttattttctc ccggggatga ggtaactccg gcaatccctg cgcttctggc
      781 tttaatcaat tcatttacaa taaaaattgt aaaaattgca atcaatgaat ttattattat
      841 ctttttcata aaattagtta gggataaata ttaattattt ggtgctcctg ctgcaatcca
      901 aattcgtatt tgtgccagct tgcaatctga tagtttagct tgattgtatg gcatggcgtc
      961 atagcccggt aaatgattga cagatccgct gagttttccg ttatttacct gcgattttac
     1021 ctgattgtaa ttagtcaaat caggttcata attgccgctg tgacagctaa tacaactgtt
     1081 ttgcagcaat ggtaaaatta caccggagta ggttacattt gatgtatcac aaggtccttc
     1141 acaatcaatt cctattttca ttccctgatt cacccaattt tcaagaacag aaatttgtgt
     1201 ggaagacaac gcaggagctg gtggtaaagg catgcggaaa gcaccagtat ctttaacaga
     1261 ttgcaaaagt aaacttcccg aaatggttgc ttttaaatgg tcataactat ccagaactac
     1321 tccggcattg gctgcatcag catcatgaca gccggacata gcacaattgg atgccagcat
     1381 aggcaacact tcttcctcaa aacaaatgct gtcttttgca tttgttggag ggtttgtaat
     1441 ggttatcttt tccggttcat gtttgcattg ggcaaacaaa agcattacga aaaagaatac
     1501 agcaagtaaa cttttactca taaattaact tttaataaat caaatgtggt ttttgaattg
     1561 ttgtacgtac agtaaaggta tgcttaattt atagtaccca aactttaata ggtatttatt
     1621 ttgttaaaaa aaacctaatt agcttaattt gtatttagac aaattcttaa ttggaaaaat
     1681 gagtactcgt cttcaaagtg atttttaagt tcagtcaacg agtttacagt attatttttt
     1741 acataagctt gaataatatc catatcggct tcactaataa attcttttgc ttccagatct
     1801 cccagctgta tccatttcaa caagtggctt tcaatagtac taactgctag tgctcgtatg
     1861 ccggctattt cttctatact ttttccttcc tgatacaatt tcaatgtatc ccgaaaggtt
     1921 gatccttttt ctgttgtatt ttttagctga acagtttcat gttgtatctc ctgataaaat
     1981 gatgtgcctg tataaaatag actatctcca aattttaacc tttctatttc ctgcaattta
     2041 tcgacgaata ctgttgctaa attttttgct tggcgcacat agccttttgt tttgttttta
     2101 atacgcattt tttctatatg tgcttccaaa ggctttatga gttcgtcatt gattttacta
     2161 caaaaatatt gtatggattt aatgactcgc tcattcagta agattctgta tttttcatct
     2221 tccacttggt taagtatgtc tttcagttgt attaaaaatt ttgctgaaac ttcttccaaa
     2281 tttcgtactg atttttccat ttgtatggct aactccatag cttctgcttt ttccggaatt
     2341 tgtttttcgt gtattattcc ctgaaattgt aaaatatgat ctgtaatttt cctccatgaa
     2401 aatgatgtaa gcagtttctt tttagaatat tccagtttgg caagtggcaa taaagatgct
     2461 aatgcatccg gattgctttt atctgcactg tattgcaaaa tacgatcgtc tgtaaaaata
     2521 gcatctgcct gtacgggagt aagtaaacta atgccttcca gacttctgca tcggcttaga
     2581 gccacataaa cttgtccggc agcgaaagca tgcccggcat caattatcac tttatcaaat
     2641 gtcagtccct gacttttatg aatagtaact gcccaagcga gttttagcgg atattgttca
     2701 aatgttccta ttacatcttc atctatttta tcagtagtat cgttataagc atatcgcaaa
     2761 ttttcccaag tatgtgaatc tacattgatt tcgtaattat cttccacaca ttttattaca
     2821 atttcttctt cattcaaacg ggtgacagtt ccgatttttc cgttataata tcgtttttcc
     2881 tgtgttaaat cattttttgt aaacatcacc tgagcgccga ctttcaacat cagttctttg
     2941 tcggcagggt aggagctttc caaaaaatca ccatgtatat ttgcttcgaa agtatgtatt
     3001 tcagatgcca gtttactcag ttcttcctga ttgattttgt cagccttgcg gttatgtgta
     3061 gtaagcaaaa tagtgttttc tgcattttga gcggcatgtg gattgtatcg cttgtttaat
     3121 tcatctaaat cagatttatt tactgcatta ttcctgaccg cattcagcaa atctataaat
     3181 tcttcatcag tttggcgaaa taccgtttcc agttcaatat gcaaaacctg aaattctttc
     3241 attacccagg cactgaaaaa aaaaggactc tcatagaatt catttaagat tcgccattct
     3301 tcgtctttta ctaccggtgg caattgatac aaatcaccta taaatagcac ttgcacacct
     3361 ccaaaaggtt cattcatccg tcttcggaaa cttcttaaca gtaaatctat ttcatccaaa
     3421 atatcgcaac gacacatact gatttcatca ataactaaca actccatttc acgtaacaac
     3481 tgaatttttt gtcggtttaa tcgcacatgt tcaaataaag tagccggatt attgacagca
     3541 taatttctgt tttttaccgg aacatacata tttaaaggca gctgaaacaa agagtgtagc
     3601 gtaactcctc cggcattaat ggctgctaca cctgtgggtg cggcaatcac acaattcttt
     3661 tgcgtatgtt gctgaatgta ctttaaaagt gttgtttttc ctgttcccgc ctttccggtc
     3721 aaaaacacag attcagcagt attgagtata aaatctttag caatttcgta tatgtccatc
     3781 tctttgacaa gataagcaag aaaattcaaa acaaaaatca gtttttacaa aaaataaaag
     3841 ttttatcttt gcagtgcaat ggatgcattt attcttcaag ttccgggcag aattcaggaa
     3901 atattgccta ttttattaat gttcatcgta gcgatgggtt ttgttgtatt ggtaatggta
     3961 ctcacccatt ggatgggtcc caaacgcaac accaaaacga aattagaaag ttttgagtgt
     4021 ggtattgaac agcatggtaa tgcgcgtaca ccattttcag taaaatattt tttggtggcc
     4081 gtgttgtttg ttttgctcga tgtggaagtc atattcatgt atccatgggc agttaatttc
     4141 agaaaactgg gtatattagg catcaccgaa atgtttactt tcattttcat ctttttagtc
     4201 ggattttatt atattctaaa aaaaggtgca ttgaaatggg agtaaattaa aaaatggcaa
     4261 tttgcatcat tcaatctgca aatttcctta ttatactaaa ctgtaaacta aattatgagt
     4321 tcagtaattg acataaaaga agaatacaag ccgattgggt tggaagggca aggatttttt
     4381 gcaacacaat tggataaagt ggtaggatta gccagaaaaa attcactttg gcccttgccg
     4441 tttgctactt cgtgttgtgg cattgagttt atggctacta tggccgccca ctatgattta
     4501 ggtcgtttcg gctctgagcg cttatcgttt tcacctcgcc aggcagatat gttgatggtg
     4561 atgggtacta tttcaaaaaa attagctccc gttttacgcc aggtttacca gcagatggca
     4621 gaacccaaat gggtattgtg catgggagct tgtgcttgtt ccggcggtgt atttgatacc
     4681 tacagcgtgt tacaaggtat cgataatatt attccggtag atgtttatat accgggttgc
     4741 ccgccacggc ctgaacaagt actggatgga attttacaaa ttcagaaatt agccgaaagc
     4801 gaagccagaa accgcagaga atcaccggaa tataaggcca tgctacaaag gtacggattg
     4861 gaatagccct aaaatattat tatgcaaata cttaccactt ttatcgaatc tactttaaac
     4921 gagcaatttc cgggagcatt attacagatt gaagaacagt atgatttccc ggtttttatt
     4981 attgcaaatg atcaggttaa aaatgtgctg aaatttctta aagaaaataa agatacggat
     5041 tttcattttc tgaccacttt atgttgcatt cattataact taaatacggc agacaaggaa
     5101 tttgccatga tgtatcagtt gcacaatatg ccaaaaggct ggcgcattcg gattaaaaca
     5161 tttttgccta aagatgaatt aacagttcct acggtaacag atttatggcc tgcggcgaac
     5221 tggttggaaa gacaggaatt tgattttttt ggtatatttt tcgatggaca tcccaatttg
     5281 aaacgcattc tcaatatgga tgaaatgaat taccacccta tgcgaaaaga atatgcttta
     5341 gaagatttcg gaagaactga taaagacgac aaaatgtttg gacgatagtt tgtgttagtt
     5401 aattagtcta ataattaagt agtttaatag ttaatgagtt agtaatttaa ttttataatt
     5461 caaaatttat aatttctgtg agtacaccta tatataacca cgaagaaaag cagtacacta
     5521 cacttaatct gggacctacg catccagcta cacatggtat ttttcagaat gtactgacta
     5581 tggatggcga aattattgta cacgccgaac ctaccgttgg ctatattcac cgtgcatttg
     5641 aaaaattagc agaacatcgt ccttataatc agataactac gctaactgac cgcttaaatt
     5701 attgttcttc accgataaat aatatgggct ggtggctgac tgttgaaaaa ttatacggta
     5761 ttgatattcc aaaacgtgct gaatatcttc gt
//
LOCUS       DAOQII010000158         5783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_7949_length_5783_cov_5.365049, whole genome shotgun sequence.
ACCESSION   DAOQII010000158 DAOQII010000000
VERSION     DAOQII010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7949_length_5783_cov_5.365049"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLF48_08160"
     CDS             <1..840
                     /locus_tag="PLF48_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49336.1"
                     /translation="IKKLSEILLDTFRLFRKYASGYLQVFAYLLVISFIIGWSIGKHY
                     LLDNMYFNGANVYNSLLFIKVNWHSWFSIIPLLMLFSYSTYTAYIFYKEFAEQQEERA
                     MAFFQWMRFTWQQTLSNLLIQVVFLGIYSYIGFKWYILLFVLPVHFIATYALYYPYYY
                     QTTFFKRYFQTIKSEILSYIVLLLIFMFITFMIFTLVNSELYMMLYEAIALNLKETSV
                     NANMLYLTFFIFSNMVLFSFILILFNLYQAVFYFSQKEKNTAEGLLNQLEKIGTKRRI
                     YGF"
     gene            830..1561
                     /locus_tag="PLF48_08165"
     CDS             830..1561
                     /locus_tag="PLF48_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49337.1"
                     /translation="MVFKKQYNILLFILLVISLTAVAQSVTTADDAQNNSQQEYQSAP
                     VQPAHMDKKKWEALKKDLKIIEPKPEKQKETKEKTKKQKEVKPLHINPKVLAVLKWTS
                     FIMMITLLLFFVLKILGINPFRKKSDKNNLQFSLEEIEDNLDKIQLDPYIEEALKNKD
                     YRLAIRLYYLMIIQRLAFKDKIIWKKYKTNKHYLNEMRSVDSFKEFSNLTRDYERIWF
                     SEMFFQKNEYDFFQPKFVQYLHSIR"
     gene            1558..3078
                     /locus_tag="PLF48_08170"
     CDS             1558..3078
                     /locus_tag="PLF48_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49338.1"
                     /translation="MSSRRNIIILVVAVLLIFIFGLYLFATNLTEWMYTLDKDKKSPY
                     GLYVTNRLLKHQYGDNFIEIKNSVIETFRELDPKKKYNYVYISHEAFYDTAVVDTLMK
                     FVAEGNTAFIAVDDITGYLRGQILSKEYHLGLNIGYNFDDSLAFAPPDTSTVLAEETD
                     EEYIDEDEISYDEEDTADIYYDSTYAYADSADVASPDSVYYLVDSNSTDINTFFSITK
                     AMSDSLAFNFTHPAFKARHDYIYFKKFKSDTLPVRNLYFEPLTDTFHNYPLQAGDAIT
                     YGAYLRYKDTSRMNMVILKHGKGNLIILLTTLPFTNYFMRKNWGLDYAEKIFAHLPNK
                     NLIWDDVSNIYEGEEQEYHGNPELNESPLYFVLKNTQLKWAWYLILLGILIYALFRAK
                     RRQQIIPLIEPKQNTSLQYVETIGQLYFHENEHIEIAVEMRNQLLNYIRNRYQLKTND
                     MDDAFFAVWSLKSGIDEDKLRDLIADYDAVAKFKSIDNAKLADLNNKTEYFYKHCK"
     gene            3084..4088
                     /locus_tag="PLF48_08175"
     CDS             3084..4088
                     /locus_tag="PLF48_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNF49339.1"
                     /translation="MEPIENENQYESFKPQVNPDIAFVHDTSEKIKREIAKVIIGQHE
                     AVDLMLCALFTGGHILLEGVPGIAKTLIARMLAKTIHSEFSRIQFTPDLMPTDIIGTS
                     VFNMKTSEFSFRAGPVFANIILIDEINRSPAKTQSALFELMEEKQVTVDGTTYKMKYP
                     FMVLATQNPIEQEGTYRLPEAQLDRFVFRIIMNYPDLQEEKQILQRFNNDFNSRITQE
                     VNAVVNGNDILRCSNAIENIYIKDDLLNYIASIVYETRNHGDLFLGASPRASLALLKT
                     SKAMAAMSGRDFVTPDDIRSVSDAVLNHRIIPSPEKEMEGVTAQDIIKQIIQKIEVPR
                     "
     gene            4277..4666
                     /locus_tag="PLF48_08180"
     CDS             4277..4666
                     /locus_tag="PLF48_08180"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNF49340.1"
                     /translation="MINQIQKFTKNAIALELSDTFTEEDTRQIKQLFEEKINDGYDHV
                     NILIMVKDLSVLKDMSFKGFIDGEWWGIKNFGKIGRCAVVSHSGFMKTIVKVENKILH
                     FFNPSLEEKYFDHAQLDEALKFINPDT"
     gene            5081..5482
                     /locus_tag="PLF48_08185"
     CDS             5081..5482
                     /locus_tag="PLF48_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49341.1"
                     /translation="MKLEYLDDISAGGKYSGIITDELIRLYDFNNSEAKEFYNAISLE
                     IVQNENELSLESLYFIESLNCKLTLKLSNYDQGIITYDKLHFICNLSKTRYLIMLRLL
                     EPFCMDAVHGYQWLYDLDTNIDFLFSAGGTW"
BASE COUNT         2025 a          971 c          973 g         1814 t
ORIGIN      
        1 ataaaaaagt tatccgaaat attgcttgat acattcagac tgttcagaaa atatgcttcg
       61 ggatatttgc aggtatttgc ctatttacta gtcatcagtt ttatcattgg ctggagtatc
      121 ggaaagcatt acttattaga caacatgtat tttaatggag ctaatgtata caactcttta
      181 cttttcatta aagtaaactg gcatagttgg tttagtatca tccccttact gatgctcttt
      241 agttattcca cttataccgc ttatatattt tacaaagaat ttgctgaaca gcaagaggaa
      301 cgggcaatgg cattttttca atggatgcgg tttacatggc agcaaacact aagcaatctg
      361 ctaatacaag ttgtgttttt aggtatttat tcctacatag gattcaagtg gtatatcctg
      421 ctctttgtat tacctgtaca ttttattgct acttatgcac tgtactatcc ttattattat
      481 caaaccacat ttttcaaacg atactttcaa actataaaaa gcgaaatact tagttatatt
      541 gtactactgc tgatatttat gttcattact tttatgattt ttacacttgt aaattccgaa
      601 ctgtatatga tgctttatga agccattgcc ttgaacttga aagaaaccag tgtaaatgcc
      661 aatatgttat atctgacgtt ttttattttc tcaaatatgg ttctgtttag ctttatttta
      721 atcttgttta atttatatca agccgtattt tatttttcgc agaaagaaaa aaacacggcc
      781 gaaggattac ttaatcaact ggaaaaaata ggaacaaaaa ggagaattta tggtttttaa
      841 aaaacaatac aacatactcc tttttatttt attagttatc agcttaactg cggtagcaca
      901 atcggtaact actgcagatg atgcacaaaa taatagtcag caagagtatc agtctgcgcc
      961 ggtgcagcct gctcacatgg ataaaaaaaa gtgggaagca ttaaaaaaag atttgaaaat
     1021 aattgaacct aaacctgaaa aacaaaaaga aactaaagaa aaaactaaaa aacaaaaaga
     1081 agtaaaacca cttcatatca atcccaaagt actggcagtg ttaaaatgga cttcctttat
     1141 tatgatgatt acgttgttgt tgttttttgt attaaaaata ttgggaataa atccattccg
     1201 taaaaaatct gacaaaaaca atttgcagtt ctcactggaa gaaattgaag ataatctgga
     1261 taagattcag ctggatcctt atattgaaga agcgttaaaa aataaggatt atcgtttggc
     1321 tattcggctt tattatttga tgatcattca gcgcttggct ttcaaggata aaatcatatg
     1381 gaagaaatac aaaaccaaca agcactatct gaatgaaatg cgcagtgtgg actcattcaa
     1441 agaattcagc aatctcaccc gagattatga gcgtatttgg tttagcgaaa tgttttttca
     1501 aaaaaatgag tacgattttt tccagcctaa atttgttcaa tatttacatt ctatacgttg
     1561 agttcaagaa gaaatataat catactcgta gttgccgtat tgctcatttt catcttcggt
     1621 ctatatttgt ttgctacaaa tctgacagaa tggatgtaca ctttagataa agacaaaaaa
     1681 tcaccgtatg gactttatgt caccaacaga ctgctcaaac atcaatacgg agataatttt
     1741 atcgaaataa aaaattcggt cattgaaaca ttccgcgaac tcgatccaaa gaaaaaatac
     1801 aactatgtat atatcagcca cgaggccttt tacgatacag cagtggtaga cacgctgatg
     1861 aaatttgtag ccgaaggaaa tacggctttc attgcagtgg atgatattac gggatattta
     1921 cgcggacaaa tcctgtcaaa agaatatcat ctggggttga atatcggcta taactttgat
     1981 gattcacttg cttttgcacc acctgatacg tcaacggtgc ttgcagaaga aacagatgaa
     2041 gaatatattg acgaagacga aatcagctat gacgaagaag ataccgctga tatttattac
     2101 gattcaactt atgcctatgc tgatagtgcc gacgtggcat cccctgacag tgtttattat
     2161 ctggtagact cgaacagtac agatatcaat acctttttct ctataacaaa agcgatgtca
     2221 gactctttgg catttaactt cacccatcct gcattcaaag ccagacatga ttatatttat
     2281 ttcaaaaaat tcaagagtga tacgttgccg gttcgcaacc tctattttga accgcttact
     2341 gatacctttc acaactaccc tttgcaagcc ggagatgcca taacatatgg tgcatacttg
     2401 cgctataagg atacatcacg tatgaatatg gttatcttaa aacatggaaa aggcaatctt
     2461 atcatactgc tcaccacttt gccatttacc aattatttta tgcgaaagaa ttgggggctg
     2521 gattatgcag aaaaaatatt cgcacacttg ccaaataaaa acttaatctg ggatgatgtg
     2581 tcaaatattt atgaaggaga agaacaagaa tatcacggca accctgaact taatgaatct
     2641 cctctttatt ttgtactgaa aaatacacag cttaaatggg cttggtattt gatattgttg
     2701 ggcatactta tatacgcact tttcagggca aaacgccgtc agcaaatcat tccgcttatc
     2761 gaacctaagc aaaatacatc acttcaatat gtggaaacga taggccagct ttattttcat
     2821 gaaaatgaac atatcgaaat tgctgttgaa atgcgcaacc agttgctgaa ctatatccgc
     2881 aatcgttatc aactgaaaac aaatgatatg gatgatgctt tttttgccgt atggagtctc
     2941 aaatcaggaa tagatgaaga taaattgcgt gatttaattg ctgattatga tgctgttgct
     3001 aaatttaaaa gtattgataa cgcaaaactg gctgacctca ataataaaac tgaatatttt
     3061 tataaacatt gtaaataaat caaatggaac caatagaaaa cgaaaatcaa tacgaatcgt
     3121 tcaaaccgca agtgaatccg gatattgcat ttgtacacga caccagcgaa aaaataaaaa
     3181 gagaaatagc caaagtgatt atcggacaac atgaagccgt ggatttaatg ctctgtgcct
     3241 tgtttacagg cggacatatc ttgctggaag gtgtacccgg cattgcgaaa acattgatag
     3301 cccgtatgct ggcgaaaact attcattctg aattttcgcg gattcagttc acgcccgatt
     3361 tgatgccaac cgacattatc ggtacttctg tgtttaatat gaaaacttct gaattttcat
     3421 tcagagcagg gcctgtattt gccaatatca ttttaataga tgaaatcaac cgttctccgg
     3481 ctaagacaca gtctgcctta ttcgagctga tggaagaaaa acaagtaacc gtggacggca
     3541 ccacctacaa aatgaaatat ccgtttatgg tattggccac acaaaatccc attgagcaag
     3601 aaggtactta ccgtttaccc gaagcacagt tagaccgttt tgtatttcgt attatcatga
     3661 attatcctga tttgcaagaa gaaaaacaaa ttttgcaacg cttcaacaac gacttcaact
     3721 ctcgcattac gcaagaagta aatgcagtag taaacggcaa tgatattttg cgatgcagca
     3781 atgcgattga aaatatctac atcaaagatg atttgttgaa ttatattgcc agtattgttt
     3841 atgaaacacg caaccacggc gatttattct taggtgcatc acctcgtgct tcactggctt
     3901 tactgaaaac atcaaaagcg atggcagcca tgagtggcag agactttgta acacctgatg
     3961 atatacgttc ggtatcagat gctgtactca accaccgcat cattccatca cctgaaaaag
     4021 aaatggaagg cgtaaccgca caagatatca tcaaacaaat tatacaaaaa atagaagtgc
     4081 ctagataaga tgaaagtagt gagtagctag tattgggtat tgagtattga tattggaaaa
     4141 tgtgtaatgt gatataagtt gtaatatgct tcagaaataa attctattat agtaagacct
     4201 tcagtctgaa tggagtttga tgcaaattga gtaatttgaa acaaatcaaa caaataatat
     4261 aattaaaaat aaacacatga taaatcaaat tcaaaaattc actaaaaatg ctattgcatt
     4321 ggaattgtcg gatacattta cagaagaaga tactaggcaa atcaagcaac tttttgaaga
     4381 aaaaataaat gatgggtacg accatgtgaa tatattgatc atggtaaaag atttgtctgt
     4441 gcttaaggac atgagtttta aaggctttat agatggagaa tggtggggaa ttaaaaattt
     4501 tggtaaaatc ggtcgttgtg cagtggtatc gcattcagga tttatgaaaa ccattgtcaa
     4561 agtagaaaac aaaatacttc actttttcaa tccgtcatta gaagaaaaat attttgatca
     4621 tgcccaatta gacgaagcgt taaaatttat aaatcctgat acataaaaaa ctgattagcc
     4681 aattagaaat gatgcacatc cttatccaaa agttgggcac agtacaaaac atatctatat
     4741 aattacttac aagaataaaa taaattatta atatatattt attatatata tatatatacg
     4801 aagctctacc ctagattgat ttagttgtat atagcattaa atctaagtaa atttagcata
     4861 ctccataatg cattaattgc ttatgcttaa catataataa atgcaattta ttttcaatta
     4921 tttataagta ttaatatatt tatttatttt atgataataa aatttatcca cccaaaaatt
     4981 catcagcttt gctattcatt aactaaaaac taactaaatt taactaacaa atctatctca
     5041 ctgagtttcg tatcttaaat ttcagtttaa cagtttcaaa atgaaattag aataccttga
     5101 tgatattagc gccggaggaa aatattcggg tataattacc gatgaattga taagacttta
     5161 tgattttaac aattctgaag caaaggaatt ttataatgct ataagcctgg aaatagtaca
     5221 aaatgaaaat gaattatctt tggaaagttt gtattttatt gagtcattaa actgcaaact
     5281 gacactcaaa ctttcaaatt atgaccaagg cattataact tatgacaagc tccatttcat
     5341 ttgcaacttg tcaaaaacca gatatttgat tatgttaaga ttattggaac cattttgcat
     5401 ggatgctgta catggttacc agtggctata tgacctcgat accaacattg actttctgtt
     5461 ttcagcaggt ggtacttggt aataaacatt tcctcttgta attcagcatc ttacaacatt
     5521 attactgcct ctatctgttt cccaatgcta atttcgagca tttttttctc attatttttt
     5581 attataaaat tatctgaaaa tgccgtacct ttgccctctg aaaatttttg aataatggct
     5641 aaaaagaaac agattgctca gcaacaagag cacgatatta ttgaagaaag caacgaaaaa
     5701 ctaaccggtt ttgtcgaaaa tatcgaatat tttttagaac gctactataa aatcatattg
     5761 ggcgttgtgg ccggcatcgt att
//
LOCUS       DAOQII010000159         5745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8026_length_5745_cov_4.379613, whole genome shotgun sequence.
ACCESSION   DAOQII010000159 DAOQII010000000
VERSION     DAOQII010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8026_length_5745_cov_4.379613"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..3247
                     /locus_tag="PLF48_08190"
     CDS             104..3247
                     /locus_tag="PLF48_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49342.1"
                     /translation="MHPSNLKKLILFLGALLLLSSVFGAQKKKKSNSQYEKPNILKRA
                     WGDVTTRNNYYFNASELYKELVHNYEFSRVINYSNLLPFYYHDSSDFSAYQGDLETIE
                     KKVGIVLQLHDYSRWRDNAYLLLGKSQFLRKQYDTSLTTFQYIVTTMKPGMMNNKLEF
                     SNKDRLKYIRKRQKELEKAAGKKKQIIEFQYKKQQEEAKEKAEDARDKQKAAIDKKKK
                     ELEEIIKAKKKIIALQKKGKKIPPELIAKAKGKSSSLDTTVMKANTSVKKSDKPNFDP
                     KLPYVLMGDQYVKNPYYKDPNDDKQIQSGADSLTYKQEAKKDKMSFWEKIKHKPSRPD
                     AIVWMSKTLIQLHNYADARSMIAYGKSLRKLTKKQRREFHLVEAYYFTSRNDYLPATE
                     SLENALLYIKKKKNKAYYEYLLAQLYEKNNQALDALDYYKKASKHTKDDELHLYAQIQ
                     QARLYVIHPELGSEDVPKMLNKLVKRNKYSDQADEVYYALSRYYYYVKDTSGAITSLL
                     KAIKYGKDDLQQKGKSYLKLGEIYYDKEDYYTAARYYDSAVAYLPNTTPDYAEIVSRK
                     SVLGNLAQYKKTINEQDSLQRLGKMSPKELDEYLAQLAKQEKKKNRFSSDEAINNGTT
                     NTGLAATSDVSNGLWYFYNPEARSRGYNEFKQIWGDRKLEDYWRRSNKSLTIDESLQQ
                     LNTNNTVDTTKNKTKSTKDIAANPEMQIPKTPEEFMASDKLIAKALFGTGEIFKNKLN
                     NLPKAKAAFDELISRFPNSDNDAKAHYYLYLIYTEQNLNGLAQKEKDYILQNYPMSEV
                     AEVIQNMNKPKTQGESEETSAKFYASTYKSFLDGNYNDVIKNNSLAKLKYPSAFEIPQ
                     FEFLNAVSFGKLKQYNEYKKALSEIIAKYPTGEVKEKAQTYLMTYLQIENSLVDTTPE
                     IAVNTLQTYVDSSVSWGYDTTGLIFLIELKDPYIRINDVIKNVENFNNKNFKDDKVKV
                     NTHFIGNTAFIQIKRFDGLALAKKYSTLIQANPTLILGQGNEFKADYYIVAPSNFRKI
                     STMDKLKSYSKYYIENY"
     gene            3250..5457
                     /locus_tag="PLF48_08195"
     CDS             3250..5457
                     /locus_tag="PLF48_08195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNF49343.1"
                     /translation="MKVLHWLSYPKNWFKLTAFFWLAVILFFVLVRIGVFGKLPDISD
                     LDNPTTNVASEIYSADSVLIGKYFSSNRSNVNFNELSPWIEKALVATEDQRFYEHSGI
                     DYQRTVSALLFLGTRGGGSTITQQLSRLMFHNRSANKFVAIFQKAKEYIISIMLERRY
                     TKHEIMAMYLNYVDFLNNADGIKSAARIYFGKQPKDLNLEESAMFVGMLQNPAYFNPK
                     RFELRTRHRRATVLRLMAEQGVITQAQFEELKDKPIVLNFHPESPNDGIAPYFRASLA
                     EWLKKWVKDNKKPDGTEWNIYTDGLKIYTTLDTRMQMHAEKAAIDNIRRFGVGNYVSI
                     WDKPKGRRAILAAIKNSADYDKVKDKSQEEIINYYKTTRRKMTVFSYSKGEKDTTMSL
                     WDSIVYYKCFMQTAFIAMETNTGFVKAWVGGPNFEFFKYDHASPNVKRQVGSTFKPIQ
                     YALAVDNGWSPCMVIPTGPITIRYHGTVWTPRGSGSPGNMRHCLARSINTAAAYMMKN
                     FGPEALVDLAYKMGITTKLPVVPSLCLGSADLSLYEMLAVYSTFPNAGISTKPQFLMR
                     IEDKDGNIIQSFTPDMKEVLNDNTAYKMCQIMKGPVSAGGTAASLQGYGIGVKDMAGK
                     TGTTNGNTDAWFIGYTPQLVAGVWVGYDDPILKAGQGNHTAAPAWGRFMSYVYNDPKL
                     KLDKSMKFFEPQDKELLKDICDEGEEFLIGSDGGSYGEVDEIMEDKSEYDLGN"
     gene            5548..>5745
                     /locus_tag="PLF48_08200"
     CDS             5548..>5745
                     /locus_tag="PLF48_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNF49344.1"
                     /translation="MKFFIDTANLEQIKEGVSLGILDGCTTNPSLMAKEGITGEENVL
                     NHYRKICELVDGDVSAEVISTD"
BASE COUNT         2068 a          959 c         1126 g         1592 t
ORIGIN      
        1 gccagttcca ttttttcaac acaacacgta tttaaacaaa attcactatt gaatataaaa
       61 taattttaag aaattgcgtt aaaattagcg aattaaagcg agcatgcatc catctaatct
      121 aaaaaaactc atactcttcc ttggggcttt acttttattg tcttcagttt ttggtgcgca
      181 aaaaaagaaa aagtctaata gtcaatatga aaagcccaat attttaaagc gtgcttgggg
      241 ggatgtcact acacgcaaca actactattt taatgccagt gagctataca aagagctggt
      301 gcataattat gagtttagcc gtgtaatcaa ttattccaat ttgttgccgt tttattatca
      361 cgattcatcc gatttttccg cttatcaagg cgatttggaa accatagaaa aaaaggtggg
      421 aatcgttttg cagttgcatg attacagtcg ctggcgcgat aatgcctatt tgctgctggg
      481 caaatctcaa tttttgcgta agcagtacga cacctcactt accacctttc agtatatcgt
      541 taccacaatg aaacccggca tgatgaataa taagctggag ttttcaaata aagaccgatt
      601 gaagtatatc agaaagcgtc aaaaagaact ggaaaaagca gcgggtaaga aaaagcaaat
      661 tatagagttt caatacaaaa aacaacagga agaagccaag gaaaaagcag aagatgcacg
      721 cgataaacaa aaagcagcta ttgataaaaa gaaaaaagaa cttgaagaaa ttataaaagc
      781 caagaaaaaa attattgctt tacaaaagaa aggcaaaaaa attccaccag agttgattgc
      841 caaagcaaaa gggaagtctt cttcattgga tactaccgtt atgaaagcca acacatctgt
      901 gaaaaaatcg gataagccta attttgaccc gaaattacct tatgtactaa tgggtgatca
      961 gtatgtaaaa aatccttatt ataaagaccc taatgatgat aaacaaattc aaagcggtgc
     1021 cgatagttta acttataaac aagaggctaa gaaggataaa atgtcttttt gggaaaagat
     1081 aaaacataaa ccatcacgac ctgatgcaat agtatggatg tcaaaaacat taatacagct
     1141 tcataattat gccgatgccc gaagtatgat tgcttacggc aaatcgctga gaaaactaac
     1201 caaaaagcaa agacgagaat ttcatttggt ggaggcttat tattttacca gccgaaatga
     1261 ttatttgccg gcaaccgaaa gtttggaaaa tgcactgctc tacataaaga aaaagaaaaa
     1321 taaagcctat tatgaatatt tgcttgcaca actttatgaa aaaaacaatc aggctttaga
     1381 tgctttggat tactataaaa aagcatcaaa acatacaaaa gatgatgaac tgcatttata
     1441 tgcacaaatt cagcaagcac gtttatatgt tatccatccg gaattaggca gtgaggatgt
     1501 gcccaagatg ctgaataaac tggtgaaaag aaataaatac agcgatcagg cagatgaagt
     1561 atattatgca ctttcccgct attattatta tgtaaaagat acatccggtg ctattacatc
     1621 attgttgaaa gcaattaaat atggcaaaga tgatctgcaa caaaaaggca agtcgtattt
     1681 aaaattgggt gaaatttatt acgataaaga agactattat actgccgccc gttattatga
     1741 cagtgcagta gcctatttac ccaatacgac accggattat gctgaaatcg tgtcgcgcaa
     1801 gtcggtattg ggcaatttgg cgcagtataa aaaaacgatt aatgaacaag acagtcttca
     1861 acgtctgggc aaaatgtctc cgaaagaatt ggatgaatat ttggcgcagt tagcaaaaca
     1921 agagaaaaag aaaaatcgtt tcagttcaga cgaggctata aacaacggaa cgacaaatac
     1981 aggactggct gctacatcag atgtatcaaa cgggttgtgg tatttctata atccggaagc
     2041 aagaagcaga gggtataatg agtttaagca gatttggggc gaccgaaaac tcgaagatta
     2101 ctggcgacga agtaataaaa gtttgacaat tgatgaaagt ctgcaacagc tgaataccaa
     2161 taatacggtg gatacaacta aaaataaaac aaaatcgaca aaagatattg cagcaaaccc
     2221 tgaaatgcaa atacctaaaa ccccggaaga atttatggct tcggataaac tgattgcaaa
     2281 agcattattt ggtaccggcg agatatttaa aaacaaactc aataatttac ccaaagcaaa
     2341 agctgccttt gatgagttga tcagtcgttt tccgaatagt gataatgacg caaaagcaca
     2401 ctattattta tacttgattt acactgaaca aaacctgaat ggtttagcac agaaagaaaa
     2461 agattatatc ctgcaaaatt acccgatgtc agaagtagca gaagtcatcc aaaatatgaa
     2521 taaaccaaaa acacagggag agagtgaaga aacatcggct aaattttatg cttccacata
     2581 caagtcgttt ttagatggca attataacga tgtaatcaag aataattctt tagcaaaact
     2641 aaaatatccg tcagcatttg aaattccaca gtttgaattt ttgaatgccg tatcattcgg
     2701 taagctgaaa caatacaatg agtacaaaaa agcgctttct gaaattatag ccaaatatcc
     2761 tacgggtgaa gtgaaagaaa aagcgcagac gtatcttatg acttatttgc aaattgaaaa
     2821 ttcattagtc gataccactc cggaaattgc cgtaaatact ttacagacct atgtagattc
     2881 aagtgtgtct tggggttacg atacgacagg gctgattttc ttaattgaac tgaaagaccc
     2941 ttatatccgc attaatgacg tgataaaaaa tgtggaaaat ttcaataaca agaatttcaa
     3001 agacgataaa gtaaaagtga atacacattt tatcggcaat actgcattta tacaaatcaa
     3061 acgctttgac ggattggctt tagcaaaaaa atactccacg ctgatacagg ctaatcccac
     3121 gctaattttg ggtcagggca atgaatttaa agcagattat tatatcgtag caccatccaa
     3181 tttcaggaaa atcagcacga tggataaact aaaaagttac tctaaatact atatcgaaaa
     3241 ttattaagca tgaaagtttt gcattggtta tcatatccga aaaattggtt taaacttacc
     3301 gcattttttt ggttggctgt tattctgttt tttgttttag taagaatagg cgtatttggt
     3361 aaattacctg atatatctga tttagataat ccgactacaa acgtagcttc cgaaatttat
     3421 tcagctgatt ctgttttaat aggtaaatat ttttccagca acagaagtaa tgtcaatttt
     3481 aatgaattat caccttggat agagaaggca cttgttgcca ctgaagatca acgcttttat
     3541 gagcattcag gaattgatta ccaacgtacc gtttctgcct tattattttt agggactaga
     3601 ggaggtggca gtaccatcac tcaacaatta tcaaggttaa tgtttcataa tcgctctgct
     3661 aataaatttg tagccatttt tcaaaaagca aaagaatata ttatttctat catgttagaa
     3721 agacgatata ctaaacatga gataatggcc atgtacttaa actatgttga ttttcttaat
     3781 aatgccgatg ggattaaaag tgcagctcgt atttatttcg gaaaacaacc taaagatttg
     3841 aatttagaag agtcggcaat gtttgtgggt atgttgcaaa atccggcata tttcaatccc
     3901 aaaagatttg aattgcgaac acgtcatcgt cgggctaccg tattgcgcct catggctgag
     3961 caaggtgtaa ttactcaagc tcaatttgaa gaattgaaag ataaacctat tgtgcttaat
     4021 ttccatccgg aatctcctaa tgatggtatt gcaccctact tcagggcttc tcttgccgag
     4081 tggttaaaaa aatgggttaa agacaataaa aaaccggatg gcacagaatg gaatatttac
     4141 acagatggtt taaaaatata taccacatta gatacgcgta tgcaaatgca tgcagaaaaa
     4201 gccgcaatcg ataatattcg ccgttttggt gtcggtaatt atgtatcaat ctgggataaa
     4261 cccaaaggca gaagagctat actggctgcc attaaaaatt cagcagatta tgacaaagta
     4321 aaagataaat ctcaagaaga aattatcaat tattataaaa cgacgagaag aaaaatgacg
     4381 gtcttttcat actccaaagg ggagaaagat actactatgt cattatggga ttctatcgtg
     4441 tattacaaat gttttatgca aacggcattt attgctatgg aaaccaatac cggatttgtt
     4501 aaagcctggg taggtggtcc aaattttgaa ttttttaaat acgatcacgc cagcccgaat
     4561 gtgaaaagac aagtgggttc aacatttaaa cctattcagt atgcattggc agtagataat
     4621 ggatggtcgc cttgtatggt aatacctaca ggcccaatta ccattcgtta tcacggtacg
     4681 gtatggacac ctcgtggcag cggcagcccc ggtaatatga ggcattgcct tgcccgctct
     4741 atcaatacgg cagcggcata tatgatgaaa aatttcggtc ccgaagcctt ggttgatttg
     4801 gcttataaaa tgggtattac tacaaaattg cctgtcgtac cttccctttg tctcggttct
     4861 gcagatttat cactgtatga aatgttagcc gtttattcca cttttcctaa cgcaggcatc
     4921 agtaccaagc ctcaattttt aatgcgtatt gaagacaaag atggaaacat catacaaagt
     4981 tttaccccgg atatgaaaga agtactgaat gacaataccg cttataaaat gtgtcagatt
     5041 atgaaaggac cggtcagtgc aggcggtacg gcggcaagtt tgcaaggtta tggcattggt
     5101 gtaaaagata tggcggggaa aacaggaaca accaatggaa atactgatgc ctggtttatc
     5161 ggatacacac cacagttagt tgcaggggtg tgggtaggct atgatgatcc tattttaaaa
     5221 gccggacaag gtaatcatac ggcagctccg gcatggggaa ggtttatgag ctatgtttat
     5281 aatgatccta aattgaaatt agataaaagc atgaagttct ttgaaccgca agataaagag
     5341 ctgctgaaag atatctgtga cgaaggagag gagttcctta tcggatctga cggtggaagc
     5401 tatggcgagg tagatgaaat tatggaagat aaatcagaat atgatcttgg aaattaataa
     5461 actgtaatca attcaaacag gatgtatttc gatttaccaa gtttcaagaa ctaaaataat
     5521 cgtattattg cacaaaatta aaaaggcatg aaatttttta tagatacagc aaatttagaa
     5581 caaataaaag agggcgtgtc tttgggtatt ttagatggat gcactactaa tccttcgctt
     5641 atggcaaaag aaggaattac cggagaagaa aatgtactta atcattatag aaaaatatgc
     5701 gagctggtcg atggggatgt aagcgcagaa gtaatttcta cggat
//
LOCUS       DAOQII010000160         5731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8054_length_5731_cov_4.157505, whole genome shotgun sequence.
ACCESSION   DAOQII010000160 DAOQII010000000
VERSION     DAOQII010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8054_length_5731_cov_4.157505"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1057)
                     /gene="era"
                     /locus_tag="PLF48_08205"
     CDS             complement(173..1057)
                     /gene="era"
                     /locus_tag="PLF48_08205"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNF49345.1"
                     /translation="MAHKSGYINILGKPNVGKSTLMNAWVGEKISIITSKAQTTRHRI
                     LGILNGEDFQLVISDLPGIIQPAYKMQESMMDYVYTSLDDADVFIYMAEAGDKPDTQP
                     TVYQKIKDLKTPTVLLLNKIDLFQQKEVEDAIALWQADFPKAVVLPVSAQHKFNIESL
                     MHFVLEHIPESAPYFEKDALTDKPERFFVTEIVREKILLNYKQEIPYSCEVVCTSFKD
                     SDSIIRIHVDIYVERDSQKPILIGKNGEMLKRVGTQARKDLEAFFGKQVFLQTFVKVS
                     DDWRNNDSMLKKFGYKES"
     gene            1164..2765
                     /locus_tag="PLF48_08210"
     CDS             1164..2765
                     /locus_tag="PLF48_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF49346.1"
                     /translation="MNINKAYLPLFAALILAFGIYAGYKIQERNTARFSFLSSKTNQS
                     KLEYILSLIDSRYVDTVDTKKLYDETLNEMLAKLDPHSVYLPPVEMKHEEEIMDGNFD
                     GIGIEFSLQKDTIYVNTPISGGPSEKAGINAGDKIIFIDDSSAAGIKITNEMVMKRLK
                     GPKGTKVKVGVLKAGTRKIVNYTIVRDKIPIYSVDAGFMLNKEVGYIKINRFAKTTYQ
                     EFMQKLEALQKQGMNKLVLDLRQNPGGFMDQAIQIADEFLEGNKTIVVTQGRTMPKTT
                     DKAQNMGLFETGKLVILVDEGSASASEIVSGAIQDWDRGVIIGRRTFGKGLVQQEYEI
                     GDGSSVRLTVAKYYTPSGRSIQSPYTEGAEAYYMDAFKRANHEAFSADSIQQNKKQVY
                     HTLIKNRTIYGGGGITPDIFIALDTSMLNPFVQKVAETNALQEFVFNYYNNQKTALTV
                     YKNEQNFNTGFKVDDLLYQQFVTFAQSKGANAAELKYAAVSRNYLSEKIKSYLARQLY
                     NNEGYYYIAAQNDKMVQRAVQELMK"
     gene            3378..4613
                     /locus_tag="PLF48_08215"
     CDS             3378..4613
                     /locus_tag="PLF48_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF49347.1"
                     /translation="MKKRSILFVTVMVFSLAKLHAQTDAERIKALEEKVDLLTKQVQA
                     QQIQVQQEDTTIAIITKEEKKSMEEKWEEVKSRIPFKISGSVDAYVQTNFAGKHTNTV
                     LNEVFPTKANSFELGMLNLMMSKEIKKVGFMADIGFGPRAEAANNTLYANTILAIKQL
                     YMTYAPTDWVKFTLGNFSTYFGYELIEPQNNFHYSTSLAFQNGPFYHTGFKANFTKKN
                     WNVLLGFFDDTDTKSDDDRNKYVGAQVGYTGSKVSTCLNFIGGNEPDTLFQRRYKFAL
                     GSTTSATLGKNGKGTLAIDLAYYRWLLKVNKKDDAKSNAQFASVYLYGKYAITDKAAI
                     GARIGYLYNGDYAAPFVAGSSKQYADFTLTSSYTIGGMLRLVPEFRLDYAKDKVFIGH
                     NGEASNLQYRLLLAAIFTF"
     gene            4765..5652
                     /locus_tag="PLF48_08220"
     CDS             4765..5652
                     /locus_tag="PLF48_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNF49348.1"
                     /translation="MSKTTLVIMAAGIGSRYGGVKQADAFGPNGEWLMDYAIYDAIQA
                     GFSDVVIITRNDLKDLLDQHIQDVWQDKIKVQYAIQAAPEGVSSGRTKPWGTGQAILA
                     TKDLVNNPFVCINADDFYGREAYQSIHQFLSNLDNDAPTFCLVGYPLENTLSAIGTVS
                     RGVCTLNENNELVAITEMTKIDLADGKLRNENDDKSFTEIPFHSFASMNFWGFTCALF
                     PELESQWNSFFAENIDELKAEYLIPTVVSKMMNEGKIKVKLLPDGKDWCGVTYSEEKD
                     LVIAALRKKIADGVYPEKL"
BASE COUNT         1944 a          991 c         1079 g         1717 t
ORIGIN      
        1 taatatgctt gattgcagta ctgtacagag ctttaaaaat gaatactgct gaagttaata
       61 aggaaaaata ctatcaaaaa acactaaaat tatttatatt tggtatcagc atttcttttg
      121 cgggagtgct tatagatatg ttcacaaatt ttgttcgtga gtaaatcaca aactagcttt
      181 ctttatagcc gaattttttc agcatactgt cattattgcg ccagtcgtcc gatactttga
      241 caaaggtttg taaaaacact tgtttgccga aaaaggcttc caaatctttg cgtgcctgtg
      301 tacccacacg tttcagcatt tcgccatttt tgccaatcaa tatcggcttt tgcgaatcgc
      361 gttctacata aatatccaca tgaatgcgaa taatgctatc gctgtcttta aatgaagtac
      421 aaacaacttc gcaactgtaa ggaatttcct gtttataatt cagtaatatt ttttctcgca
      481 ctatttccgt aacaaaaaag cgctcaggct tatcagtcag tgcatctttt tcaaaataag
      541 gagctgattc agggatatgt tccaaaacaa aatgcatcag cgactcaata ttgaatttat
      601 gctgtgccga aaccggcaat accacagctt ttggaaaatc tgcctgccac aaagcaatgg
      661 catcttccac ttctttttgc tgaaacaaat caattttatt caataataaa acagtgggag
      721 ttttgaggtc ttttattttc tgatacactg taggctgcgt atcgggtttg tcgcccgctt
      781 cagccatata gataaataca tccgcatcat ccagtgaagt atagacataa tccatcatgg
      841 attcctgcat tttataagca ggctgaatga tacccggcaa gtcagaaatc accaattgaa
      901 aatcttcgcc attgaggatt cccaaaatgc gatgtcgggt agtttgagct ttagaagtga
      961 taatagaaat cttctctcct acccaggcat tcatcaaggt agatttgccc acattgggtt
     1021 tgcccaaaat attgatatat ccagatttgt gtgccatgat tttgtgtggc aataaaggta
     1081 cgtattttaa cttgtttgaa taaacaccga gcactttaaa aaatttaaga ttactttcat
     1141 ttttaaaatt ttacatttgc tcaatgaata tcaacaaggc ttatttacct ctttttgctg
     1201 ccctcatttt ggcgtttggc atctatgcag gctataaaat acaagaaaga aataccgctc
     1261 gtttcagctt tttatcgtct aaaacgaatc agtccaaact ggaatatatc ctcagcttaa
     1321 tagattccag atatgtagat acggtagata ccaaaaaact ctacgatgaa acgctcaatg
     1381 aaatgctggc aaaattagac ccgcacagtg tttatctgcc tcctgtagaa atgaaacatg
     1441 aagaagaaat tatggatggc aatttcgacg gtatcggcat cgaattttcc ttacaaaaag
     1501 ataccattta tgtgaatacc cctattagcg gcgggccttc cgaaaaagcc ggaatcaatg
     1561 caggcgataa aatcattttt attgatgact caagtgctgc aggaattaaa ataaccaacg
     1621 agatggtaat gaaaagacta aaaggaccca aaggtaccaa agtaaaagta ggtgttttga
     1681 aagccggcac tcgtaaaatt gtaaattaca ccattgttcg ggataaaatt ccaatttatt
     1741 ctgttgatgc cggatttatg ctgaataaag aagtcggata tatcaaaatc aaccgctttg
     1801 caaaaacaac ttatcaggaa tttatgcaaa agctggaagc cttgcaaaaa caaggtatga
     1861 ataaattagt cttggacttg cgtcaaaacc cgggtggttt catggatcag gctattcaaa
     1921 ttgcggatga atttttagaa ggtaataaaa ccatagtagt aacacaggga agaacgatgc
     1981 caaagactac agacaaagca caaaacatgg gactgtttga aacgggcaaa ctggtgattt
     2041 tagtagatga aggttcggca tcagccagtg aaatagtgag tggagccata caagactggg
     2101 acagaggtgt cattattggc agacgcactt tcggcaaggg tttggttcag caagaatatg
     2161 aaattggaga cggaagttct gtaagactta cagttgcaaa gtattataca cccagtggcc
     2221 gatctataca aagtccatac acagaagggg cagaagctta ttatatggat gcctttaagc
     2281 gtgccaatca cgaagctttc agcgccgaca gcattcagca aaacaaaaaa caagtatacc
     2341 atacgctgat aaaaaacaga acaatttatg gtggcggcgg tatcacaccg gatattttta
     2401 ttgccttgga taccagtatg ctcaatccat ttgtacaaaa agtggccgaa acaaatgcac
     2461 ttcaggaatt tgtctttaat tattacaaca atcagaaaac ggcattaact gtttataaaa
     2521 atgagcaaaa tttcaataca ggcttcaaag tagatgattt gctctatcag caatttgtca
     2581 catttgcaca atctaaaggt gcgaatgctg ccgaattgaa atatgctgct gtttcccgca
     2641 attatctttc tgaaaaaata aagagttatt tagcccgtca gctatacaac aatgaaggct
     2701 attactacat agctgcacaa aacgacaaaa tggtacaaag agccgtacag gaattgatga
     2761 aataaatcaa taacaacata ttattcataa ctcgttgtat ggtttaaacc acataaaaac
     2821 ataacaattt tattcataaa tagctgtctg gtgcaaatca gtatagatag tcagacaata
     2881 acgtagtatc gcttttggta attccgaaat ttttctatat ttcctcaatc ttttagagtt
     2941 aacattttta attctacaaa aacctattca catcattgtg gtaataacta taaatcaaac
     3001 tctacaagag gttattcata agtaatctct taacaactaa gggtttaaag aaatcagtaa
     3061 tttaacaaga ttatattcaa acgtaagcaa aatcgagata aggtttttgc tgcctattgt
     3121 attataatta atccagttta cataacattt cattacaaca tagggttttg ataactgatt
     3181 catcttaaaa aatattattg tattatttta aacaaaacag gcacttaaat gcattatgta
     3241 ctaaaattat taaataattt tacgcaatta aaccatttta gtaatgtatt gctcaaaatt
     3301 tattaacttt caaaaatagg attcagatta tttcatgctt agtattgcat atagttaatt
     3361 tttaaaatct tacgcttatg aaaaaaagaa gtattttatt cgttacggtg atggttttca
     3421 gcctggcgaa actgcatgca caaacggatg ctgaacgcat taaagctctg gaagaaaaag
     3481 tagacttact tactaaacag gttcaagccc aacagataca agtacagcaa gaagatacta
     3541 ctattgccat cataacaaaa gaagagaaaa aaagtatgga agaaaaatgg gaagaagtaa
     3601 aaagcagaat tcctttcaaa ataagcggct cagtagatgc ttatgtacaa actaattttg
     3661 caggaaaaca tacaaacact gtactaaatg aagtctttcc aactaaagcc aattcttttg
     3721 aacttggcat gttgaattta atgatgagca aagagataaa gaaagttggt tttatggcag
     3781 atattggttt tggtccgcgt gccgaagctg ccaataacac gttgtatgca aacaccattt
     3841 tagccatcaa acaattatac atgacatatg ccccgacaga ctgggtgaaa tttactttag
     3901 ggaatttcag tacctacttt ggttatgaat tgatagaacc gcaaaataat ttccactatt
     3961 ccacttcgct ggcatttcaa aacggtccgt tttatcatac cgggtttaag gccaatttta
     4021 ccaagaaaaa ctggaatgtc ttgttgggat tctttgacga tactgacacc aaatctgatg
     4081 atgacagaaa taaatatgta ggtgcacaag tcggatatac aggctcaaaa gtaagtacct
     4141 gtttaaattt tataggtggt aatgagcctg atacattgtt tcagcgtaga tataaatttg
     4201 cattaggctc cactacttct gctactttag gcaaaaatgg aaaaggtacg ctggccatag
     4261 acttggcgta ttacagatgg cttttaaaag ttaacaaaaa agacgatgcc aaatcaaatg
     4321 cacaatttgc ttctgtctat ttgtacggca agtatgctat tactgataaa gcggctatag
     4381 gtgccagaat aggctatttg tataatggtg attacgcagc gccatttgtt gcaggttctt
     4441 caaaacaata tgcagatttc acgctaactt cttcttatac tattggtggc atgttgcgtt
     4501 tagtaccgga attcagatta gattatgcga aagataaagt ttttatcgga cataatggcg
     4561 aggcaagcaa cttacaatat cggttgttat tagcagcgat attcacattt taatcatatc
     4621 atttaaaaag tataataatg ttagggggcg cacctgaggt gcgccccctg acattttatc
     4681 tacaaaccat tatacaagaa tatagtcata ttagatgtac ttttttttcg ataaacattt
     4741 tttcgatttt tttatctttg cgacatgagc aaaactacat tagttatcat ggctgcaggc
     4801 atcggtagcc gttatggtgg tgtgaaacaa gcagatgcat tcggaccaaa tggagagtgg
     4861 ctgatggatt atgccatcta tgatgccatc caagccggat tttcagatgt agtcattatt
     4921 acccgaaacg acttaaaaga cctattagac cagcatattc aagatgtttg gcaagataaa
     4981 ataaaagtac aatatgctat ccaagctgct ccggaaggag taagcagtgg gcgtactaaa
     5041 ccttggggaa caggtcaggc aatattagca actaaagatt tggtcaataa tccttttgtt
     5101 tgtattaatg ccgatgattt ttatggcaga gaagcctatc agtctattca tcagttcctc
     5161 tctaatttag ataatgatgc acctactttt tgcctggttg gatatccgct tgaaaacacc
     5221 ttgtctgcta ttggaacggt gtctcgcgga gtttgcacat taaatgaaaa caatgaactg
     5281 gtagccatta ccgaaatgac aaaaatagat ttagctgatg gtaaattgcg caatgagaat
     5341 gacgataaaa gctttactga aattccattc catagttttg cttctatgaa tttctggggc
     5401 ttcacctgtg ctttatttcc tgaactggaa tctcaatgga acagcttttt tgcagaaaac
     5461 attgacgaac ttaaagccga atatttaatc ccaacagtag tttctaaaat gatgaatgaa
     5521 ggcaaaataa aagtaaaatt attacccgat ggtaaagact ggtgtggtgt aacttattca
     5581 gaagaaaaag atttagttat agctgcattg cgcaaaaaaa tagcagatgg ggtatatccg
     5641 gaaaaacttt aggagtgagt gagtagtggg tagtatgtag taagtagtaa gtagtaagta
     5701 gtgagtagtg agtagtgagt agtgagtagt g
//
LOCUS       DAOQII010000161         5707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8111_length_5707_cov_6.058563, whole genome shotgun sequence.
ACCESSION   DAOQII010000161 DAOQII010000000
VERSION     DAOQII010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8111_length_5707_cov_6.058563"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PLF48_08225"
     CDS             complement(<1..1014)
                     /locus_tag="PLF48_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49349.1"
                     /translation="MSNPITYLRVFRTANNLPSITNWYKGDTHLHSIYTQNTAEQGLP
                     LPATKDAAKLMGIDWIITTDHTSDFDNYGDGNVNNNWSKLGTEVVALNAADPTMMYIR
                     GQEVALKNSIGKLVHFLGYPSYSNPTTAGFLGDGNGDATPTNVTIDNALNSLFLSNGF
                     CYAAHPFATNDELSPLVNGGIWNLGDDDFTINGNNFPQTGGNIICNDPTITSDVLVSN
                     GNDPNKFVKDALKGGQIWNERSSMLTTDSPDDPYNIGGGHTPFAAWPNDLTQHWNRFR
                     QGLEIINFINKKGLLLKNSNDAIQNWKFYLSAGTDAHGSFNYSTTNYVQSFLGNLGGS
                     IQNN"
     gene            complement(1077..1889)
                     /locus_tag="PLF48_08230"
     CDS             complement(1077..1889)
                     /locus_tag="PLF48_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49350.1"
                     /translation="MKKLLLLLFSISTFYIFAHDHHGENKVVLSDVYLEKQIDRYLIS
                     FKIHTLEKRPVDFAKINLVVNRKVIASKNIEYITKEKKDYQISFEKGIITSDTDQVLL
                     HVVNVDGYYGNWRTQPIKIDSLPETSTYQYDNVGAYELFADAPWMMKIYNSSGVKNSV
                     PVLLILHDGDGVSYPWIQYADIYIKKSTDAAFGTPLTFNGLTNAQFQALFTNKSTADA
                     TLNRKAFDQSLAVKDASHTIAFTESTYFSHLLNIPLFKISCGMPLLTFQPHH"
     gene            complement(1936..2562)
                     /locus_tag="PLF48_08235"
     CDS             complement(1936..2562)
                     /locus_tag="PLF48_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNF49351.1"
                     /translation="MKNHVSTLIFDLGNVIINLLDDQQWYQNLGAVCKEMELMRLKNA
                     GFFDEFEKGNLTELEFLHSLKTIAINTAVTETDIIAAWNTILLDIPPSRVDMLKKLKQ
                     KYRIILLSNTNSIHISKIEDYCVSTFGENCLQTCFHHAYFSYDLHERKPDAEIYEQVK
                     WLEDFLPNETYFFDDKPENLIYPSMMGIQTFLVDQEISHILSAIGIYE"
     gene            complement(2635..3291)
                     /locus_tag="PLF48_08240"
     CDS             complement(2635..3291)
                     /locus_tag="PLF48_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF49352.1"
                     /translation="MIKILIADAHFLIRTGLTFLLSEQKDFELVGETDQPSNLIALAK
                     ATQPDVVIFDYKSGNHFSIDDIKRLKIVLPDCGILIISDDQHKESIFTAIEMGAISFL
                     TKECDKEEIIGAVRATAKGEKFLCHKIIDIIIESHVHKDEDMCKALNLSARETEIIKL
                     TAKGWQAKTIAEHLFLSTHTVYTHKKNIMKKLKINTSSEMIVYAIQNKLITEEEFSEA
                     "
     gene            3394..4581
                     /locus_tag="PLF48_08245"
     CDS             3394..4581
                     /locus_tag="PLF48_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49353.1"
                     /translation="MNNNLSFHPTLLPLSPSKDNDKLWEEVLKKYDEKQYAESVRTLV
                     VYIDKALEDKYANADKTEYFIPHGSVTVGLKISEDTLFITAPFLDISTAKQVPLLRQV
                     AQINFSPLTLSRIDLTENQLTFNFSCPLTLCEPYKIYDVLREVCINADNYDDEFITKF
                     SATHIQTPKTESLTEEQKNLAYDRIQAYAAEALSGYEQLENKRLTAFLWDVLITTLLK
                     IDYYCVPQGNLRNELEKTISFLNSKEDFYQRMSTAKDFLKKLQTTDKEKLKKDLYKIE
                     IFVPYKYNANLDAVRNNLKYAYETAEKEMKAGDNIGATFSMSYGILNLFYSYNVDTSV
                     AALLTKAMTDASEKPMQESAKILYDAIHTIMTKDNFTVSVSKVADEAQKENKGFFKKI
                     FGL"
     gene            4578..5660
                     /locus_tag="PLF48_08250"
     CDS             4578..5660
                     /locus_tag="PLF48_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNF49354.1"
                     /translation="MNDNEIKQAVYKINTASGSGSGFYLSKQNLFVTNYHVIEGNKLV
                     SLENQNKDRYRAHVVYVNPDADIAFLRSEKVLSQAAIPFENVQEVRSRDKVFVLGYPF
                     GMPYTITEGIVSNEKQLLDGKNYIQTDAAVNPGNSGGPVVNENGELVGVTTAKFTEAD
                     NVGFAIPGKVVEEELASFQENKSDQFSLKCSSCKSLIFEKTDYCPNCGANINEKIFDD
                     KETGKLSVFVESAIENMGLDPVLSRNGAEFWEFYSGSALVRIFVYERNYLYATSPINN
                     LPTGNLENLFKFICNNPVSPYKLGVYQNQIFISYRVRISDVFSDQSETVKQHLSQLIL
                     KANEMDDYFTDIFGCEKTNFAKIEQA"
BASE COUNT         1789 a          996 c         1101 g         1821 t
ORIGIN      
        1 attattttgt atagatccgc caagatttcc taaaaatgat tgcacgtaat tagtggtaga
       61 ataattaaaa gagccatgtg catccgtacc tgcagaaaga tagaatttcc agttttgtat
      121 ggcatcgtta ctgtttttaa gcagtaatcc ttttttatta ataaaattaa ttatttccaa
      181 tccttgtctg aatcgattcc aatgctgtgt taaatcatta ggccaagccg caaatggagt
      241 atgcccacct ccaatattat acgggtcatc cggagagtca gttgttaaca tggatgaacg
      301 ctcattccat atttgcccgc cttttagtgc atctttgaca aacttattag ggtcgttccc
      361 attgctgact aaaacatcgg atgttattgt cgggtcatta cagataatat tgcctccggt
      421 ttgtgggaag ttattcccgt taattgtaaa atcgtcatca cccaaattcc agattccgcc
      481 atttaccaga gggcttaact cgtcattggt ggcaaaagga tgtgcggcat agcaaaaacc
      541 attgctcaga aacagactat tcagtgcatt gtcaatagtt acatttgtgg gtgttgcatc
      601 gccgttgcca tctcccaaaa atcctgcagt agtggggttt gaatagctgg gatatcctaa
      661 aaaatggaca agtttgccaa tagagttttt tagggctact tcctgtccac ggatatacat
      721 cattgtcggg tcggctgcat ttaaggcaac tacttctgtt cctaattttg accagttgtt
      781 atttacgttt ccatcaccat aattatcaaa atcagaggta tggtcggttg tgataatcca
      841 gtcaatgccc atgagttttg cagcatcttt agttgcagga agtggcaaac cctgttcagc
      901 tgtattttgc gtatatatgc tatgtaaatg tgtatctccc ttgtaccagt ttgtaatact
      961 gggcagatta ttggcagtcc gaaaaacacg taagtaagta atcgggttgc tcaatggagt
     1021 tatgcgtatg gtatatacta cttttatatc cagtacatca gtatcagtga atgtacttaa
     1081 tgatgaggct ggaatgttaa aagtggcata ccacaggaaa ttttgaacag aggtatattt
     1141 aaaagatggg aaaagtaggt tgattccgta aatgcaatag tgtggctggc gtcttttacg
     1201 gcgagacttt ggtcaaaagc tttgcggttt aacgttgcat cagcagttga tttattagta
     1261 aacagcgcct gaaactgtgc attagtcagc ccgttgaatg taagtggtgt gccaaatgct
     1321 gcatcggtag attttttgat atagatatcg gcatactgta tccaggggta ggaaacacca
     1381 tctccatcgt ggagaataag caaaacgggt acactgtttt ttactcctga agagttatat
     1441 attttcatca tccatggagc atcagcaaat aactcataag ctcctacatt gtcgtattga
     1501 taagtggatg tttctggaag gctgtcaatt tttataggct gtgtgcgcca gttgccataa
     1561 tagccgtcaa catttaccac atgcaagagc acttggtctg tatcgctggt gattatgcct
     1621 ttctcaaagc tgatttgata atctttcttt tcttttgtaa tatactctat attcttcgaa
     1681 gctattactt tacggttaac gaccaggttt attttggcaa aatcaaccgg tcttttttca
     1741 agtgtatgaa ttttaaagga tattaaatag cggtcaattt gcttttccaa atacacatcg
     1801 cttaagacta ccttgttttc gccatgatgg tcatgtgcaa aaatgtaaaa agtggatata
     1861 ctgaaaagta aaagtaaaag tttcttcata tctgaataca aatgtataca tttttcgttt
     1921 aattatcaat atgcatcatt cgtatatacc aatagctgat aagatgtgag agatttcttg
     1981 gtcaaccaaa aaagtttgaa ttcccatcat gcttggatag attaaatttt cgggtttgtc
     2041 atcaaagaaa tatgtttcat ttggcagaaa atcctcaagc cactttactt gttcataaat
     2101 ttcagcatca ggtttccgct catgcaaatc ataagaaaaa tatgcatggt gaaaacatgt
     2161 ttgcaggcag ttttctccga atgtagacac acaatagtct tcaatctttg agatatgaat
     2221 gctgtttgta ttgctgagta atatgatgcg atatttttgc ttcagttttt tcagcatatc
     2281 tactctcgaa ggcggtatgt ctaagagaat ggtattccaa gcagcaataa tatccgtttc
     2341 ggttacagct gtattaattg ctatagtttt gaggctatgc aaaaattcca gttcggtcag
     2401 atttcctttt tcaaattcat caaagaaacc tgcattttta aggcgcatta attccatttc
     2461 cttgcagacg gcacccaagt tttgatacca ttgttggtca tccagcagat tgataattac
     2521 attgcccaaa tcaaaaataa gtgtgcttac atggtttttc ataatagaaa ttttgttgga
     2581 gttaaataca ataaaaaata ctgtatttaa tagaataaaa acagatatgt ataattacgc
     2641 ttcggaaaat tcttcctctg taatcagttt gttttggatg gcgtaaacaa tcatttctga
     2701 agaggtatta atcttcagtt ttttcatgat atttttttta tgcgtataga cggtgtgtgt
     2761 cgataaaaat aagtgttccg ctattgtttt agcctgccag ccttttgcag taagtttgat
     2821 aatttctgtc tctcttgctg ataagtttaa ggctttgcac atgtcttcat ctttatgtac
     2881 atggctttca atgatgatat ctataatctt gtgacacaaa aatttctcac cttttgcagt
     2941 tgcccggaca gcaccgataa tctcttcttt gtcacactct tttgtcagaa aactgatggc
     3001 tcccatctca attgccgtaa aaatggactc tttatgctga tcatccgaaa taattaatat
     3061 tccacaatcc ggcaaaacaa tcttcagccg tttgatatcg tcaattgaaa aatgattgcc
     3121 gcttttgtaa tcaaaaataa ctacatcagg ttgtgtggct ttagccaaag caattaagtt
     3181 gctcggttga tccgtttctc ctactaactc aaaatctttc tgctctgata ataaaaaagt
     3241 caacccggta cggattaaaa aatgtgcatc agcaattaat atctttatca ttgtggtata
     3301 atcaggtata aatttaagca aaagtggcga agtatagatt aatttagtgt aaatgtgaaa
     3361 taagtagtaa tttagcacct cctaagaata attatgaata ataacctatc ttttcatcca
     3421 acattattgc ccttatctcc atcgaaagat aatgataaac tatgggagga agtgcttaaa
     3481 aagtatgatg aaaaacaata tgcagaaagt gtaaggactc tcgttgtcta tatagataaa
     3541 gcgttggaag ataaatatgc aaatgctgac aaaactgaat attttattcc tcacggctct
     3601 gttactgtag gtttgaaaat ttcggaggat acattgttta ttaccgctcc gttcctggat
     3661 atcagcacag ccaaacaggt gcccttattg cgacaggttg cccagattaa tttcagcccg
     3721 cttacacttt cacgcataga cttaactgaa aatcagctta cattcaactt tagttgtcct
     3781 ttgacattgt gcgaacccta taagatttat gatgttttac gggaagtatg tattaatgcc
     3841 gataattatg atgatgagtt cattaccaaa ttttcagcaa cgcatattca aacgcccaaa
     3901 acagagtcgc tgactgaaga acaaaagaac cttgcttatg acaggataca agcttatgca
     3961 gctgaagcac tttccggcta tgagcaattg gaaaataaaa gactgaccgc ctttttatgg
     4021 gatgtattaa taacaacgtt gcttaaaatt gattattact gtgtgccaca aggcaatttg
     4081 cgcaatgaac tggaaaaaac gatctctttt ttaaacagta aagaagattt ctatcagcgc
     4141 atgtctaccg ctaaagattt tctgaagaaa ctgcaaacaa cagataagga aaagctgaaa
     4201 aaagatttgt ataagataga gatttttgtg ccttataaat acaatgctaa tctggacgca
     4261 gttcgcaata acctaaaata tgcctacgaa acagctgaaa aagaaatgaa ggccggtgat
     4321 aatattggcg ctacattttc tatgtcatac ggaatactta atttgtttta cagttataat
     4381 gtggatacat ctgtagccgc attgctgaca aaagccatga cggatgcatc tgaaaagcct
     4441 atgcaggagt ctgcaaaaat attatatgat gctatacaca ctattatgac taaagataat
     4501 tttactgttt ccgttagtaa agttgctgat gaagcacaaa aagaaaataa aggattcttt
     4561 aaaaagattt tcggactatg aatgacaacg aaataaaaca agccgtttat aaaattaata
     4621 ccgccagcgg aagcggtagc ggattttatc tgagcaaaca gaacctgttt gtaaccaact
     4681 atcatgtgat tgaaggcaat aagttagttt cactcgaaaa tcaaaataaa gacagatacc
     4741 gggcacatgt agtgtatgta aatccggatg cagatattgc atttttgcga agtgagaaag
     4801 tcctttctca agccgctata ccatttgaaa atgttcagga agtacgcagc cgtgataaag
     4861 tatttgtact gggatatcct tttgggatgc cctataccat tacggaaggc atcgtttcca
     4921 atgaaaaaca attattagac gggaaaaatt atatccaaac agatgcggct gtgaaccccg
     4981 gaaacagcgg agggccggta gtaaacgaaa atggcgaact ggtgggagta acgacagcta
     5041 aatttacaga agccgataat gtgggttttg ccatacccgg caaagtggtg gaagaagaac
     5101 tggcatcatt tcaagaaaat aagagcgacc agttttcatt aaagtgcagt tcttgcaaat
     5161 cattgatttt tgagaagacg gattattgtc cgaattgcgg agctaatatc aacgagaaaa
     5221 tatttgatga taaagagacc ggaaaattgt ctgtatttgt agagtcggca atagaaaaca
     5281 tgggattaga cccggtactt tctcgcaatg gtgctgagtt ttgggaattt tattcaggct
     5341 ctgcattagt gcgtattttt gtttacgaac gaaattattt gtatgctact tcgccgatta
     5401 ataatttacc taccgggaat cttgaaaact tgtttaagtt tatttgtaat aatccggtat
     5461 caccctataa attgggggtt tatcaaaatc aaatttttat ctcctacagg gtgcgtattt
     5521 ccgatgtgtt ttcagatcag agcgaaacag taaaacaaca tttaagtcag cttattctga
     5581 aagccaatga gatggatgat tattttactg acatatttgg atgtgaaaaa acaaactttg
     5641 ccaagataga acaagcttaa taagcttttt tctgtcttac tccagcttaa ctaacaggat
     5701 gtcagat
//
LOCUS       DAOQII010000162         5635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8271_length_5635_cov_4.329570, whole genome shotgun sequence.
ACCESSION   DAOQII010000162 DAOQII010000000
VERSION     DAOQII010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8271_length_5635_cov_4.329570"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1145
                     /gene="aroB"
                     /locus_tag="PLF48_08255"
     CDS             93..1145
                     /gene="aroB"
                     /locus_tag="PLF48_08255"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNF49355.1"
                     /translation="MRQLQLHDYDIQFAQDGLVLSEFLSAKNYTGIYILTDENTSALC
                     LPLIQDKISHFTEIKTNSGESHKNLVTCASIWEKLNDTHADRKAVLLNLGGGVIGDMG
                     GFAASCYKRGIDFINIPTTLLSQVDSSIGGKLGIDLNYGKNLIGLFNNPELVIISSSF
                     FKTLPQRQYVNGWAEIFKHALIKSKSQWENFRAIDIFYCEIDEIVFQSLKIKKEVVEA
                     DPFEKGVRKILNFGHTIGHAIESYSLEKTNHPLLHGEAIVIGMITESWIATQLCGLKS
                     VELDEIKTVLLRHFPKQDISDFDFETLYKLILTDKKNEQLKIKMALINEIGNCLYDIE
                     VDKDLIRRALDYYAAL"
     gene            1188..2357
                     /locus_tag="PLF48_08260"
     CDS             1188..2357
                     /locus_tag="PLF48_08260"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNF49356.1"
                     /translation="MRPFLLFIFFYCMIHLSHAVDVITGAQRTADYLPYLKNKKVGLV
                     VNQTSVIGNTHLIDSLISLGVQIKTVFAPEHGFRGTADAGEHVDNGMDKKTGIPLISL
                     YGDNKKPKPNQLKDLDIVVFDIQDVGVRFYTYISTLQYVMEACAENNKPLIVLDRPNP
                     NGHYVDGPVLDTQFRSFVGMQQIPVVYGMTIGEYARMLNGEKWLAKKAVCKLTVISCK
                     NYNHTTFYSLPVKPSPNLPNMTAIYLYPSLCLFEGTDKISLGRGTDKPFQIYGSDLFS
                     DDMLYQFTPQSREGAKSPPLMNKLCYGYDLSELPIDSLRNQKFTLRYFLQAYNQMPDK
                     NKFLNSFFNKLMGSTAFPQQLEKGMSEKAIRASWQTKLNKFKLIRKKYLLYKDFE"
     gene            2457..3275
                     /locus_tag="PLF48_08265"
     CDS             2457..3275
                     /locus_tag="PLF48_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNF49357.1"
                     /translation="MYKKHLLFLLFTSLFAWKSNAQNASANINDLIQKSFTAMANVKT
                     MTYHFYASERMDDGTYSKSDVEFKVVASPLKIFANAHEPQSAKLSYDASKSKDVKVKK
                     GLSLSLSPTNKLLMKGVHNPITRAGFGRIKMILEKSLAQRKGQDLSKYVKLAGTVMYD
                     NKECLKVVISDPDYKIIDYTVKPGQTNVWQIGSDLAISEYKIKELNGIGDDVKPGQKL
                     KIPSSYAKVTTIYFDKATNLPVFHRMEDEKGVYETYEFKNLKTGMTFTDADFQF"
     gene            complement(3342..3689)
                     /locus_tag="PLF48_08270"
     CDS             complement(3342..3689)
                     /locus_tag="PLF48_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNF49358.1"
                     /translation="MAIRITDECINCGACEPECPNNAIYEGGIEWRIADGNTVKGNYT
                     LMDGRVVDAEAKQYPVSMDLYFIVTDKCTECKGFHEEPQCAAVCPVDCCIPDENHVET
                     EAELLAKKEKLHL"
     gene            3803..4816
                     /locus_tag="PLF48_08275"
     CDS             3803..4816
                     /locus_tag="PLF48_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNF49359.1"
                     /translation="MHLNERIAAFEQLGKYILSYDEELKNAIQQAYLNNNWFTEASCE
                     MQLQTIATDYLDGVKLYNWVKNYTLLYEDKLPPRIVGIVCAGNLPLVGFHDILSVLIC
                     GYTAQIKLSEKDKELPLFLLQKLIDIQPMFASLINIVSKLENFDAIIATGTNQTAAHF
                     RTYFNKYQHIIRNHRNGIAILTGTETEEDLFNLGKDVFSYFGLGCRNVSLLFVPKNYD
                     FTSLIKAWQAFKDVLMNHNSYRNNLDYQRTIYLMKQTPMIDIDFVNMTENDSMYSPVS
                     CLHYTYYDDINEVYTYINKYEAQIQCIIGKDYLAFGKSQLPKLDEYADNTDTLAFLTQ
                     LHG"
     gene            4893..5357
                     /locus_tag="PLF48_08280"
     CDS             4893..5357
                     /locus_tag="PLF48_08280"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF49360.1"
                     /translation="MNCKTVFDDTAFKMEPVEYELDLDSDLLNLKETKLTEPVPVNKR
                     VINYLVDSLSIALIIVFFQSFGIEILGNVFQIYLLFYTYFFFMEAAFGQTIGKFVTRT
                     KVVDLNGEKPTLGRIALRSICRFIPFDPISFLFQDRVFWHDSLSKTRVVDAA"
BASE COUNT         1890 a          969 c          978 g         1798 t
ORIGIN      
        1 ccaaaagcta gacggcggtt ttgttataga aaaatagtac ttacataata gctgatggtt
       61 gaaaatgaga atagctttcc ttattttgca tcatgcgaca attacagctt catgattatg
      121 acattcaatt tgctcaggat ggtctagtac tttccgaatt tttatctgca aaaaactata
      181 ccggtatcta catattaact gatgaaaata cttctgctct gtgcttaccc ttaattcaag
      241 ataagatttc acattttaca gaaataaaaa caaatagtgg cgaatctcat aaaaatttag
      301 tgacttgtgc atctatttgg gaaaagctaa atgataccca tgcagacaga aaagcggtac
      361 tcctaaattt aggaggcgga gtaattggcg acatgggcgg ttttgcagct tcatgctata
      421 aacgcggcat tgattttata aatatcccca ctacgctatt atctcaggtt gattcatcta
      481 ttggtggaaa gctgggtatc gatctgaatt atggaaaaaa tttaatcggt ctgtttaata
      541 atcctgaact cgtaatcata tcatcctcct ttttcaaaac attgccacaa agacaatatg
      601 taaacggttg ggcagaaata tttaaacatg cacttattaa aagtaaatcc caatgggaaa
      661 actttagggc tatcgacatt ttctattgtg aaatagatga aattgttttt caatcattaa
      721 agataaaaaa ggaagtagtt gaagctgatc catttgaaaa aggagttcgc aaaatactga
      781 attttggaca tactatcgga catgctattg aatcttattc gcttgaaaaa acaaaccatc
      841 cacttttaca tggagaagca atcgtgattg gcatgattac tgaatcctgg attgctactc
      901 aactatgtgg cttgaaaagt gtagaactgg atgaaattaa aactgtctta ctcagacatt
      961 ttcccaaaca ggatatttca gattttgatt ttgaaacttt gtacaagctg attttaaccg
     1021 ataaaaaaaa tgaacagctt aagattaaaa tggctttaat taatgagata ggaaattgcc
     1081 tctatgatat cgaagtagat aaagatttga ttcgaagagc attggactac tatgcagccc
     1141 tataaaatct gcaatttatt tagcacaata tttgatactt ttaccgcatg agaccattcc
     1201 tgttgtttat ttttttctat tgcatgatac acttatctca tgctgtagat gttattaccg
     1261 gagctcaacg cactgctgat taccttccct atttaaaaaa caagaaagtt ggtctggttg
     1321 tcaaccaaac ttctgttatt ggaaatacgc atcttattga ttctttaatt tcattaggtg
     1381 tacaaataaa aactgtattt gctcccgagc acggctttcg aggtacagca gatgccgggg
     1441 aacacgtgga taacggcatg gacaaaaaaa caggaattcc actgatttct ctttatggag
     1501 ataataagaa accaaaacct aaccagctga aagatctgga tattgttgtt tttgatattc
     1561 aggatgtagg ggtacggttt tatacctata tatccaccct gcaatatgtc atggaagcgt
     1621 gtgcagaaaa taacaaacca ttgattgtac tggacaggcc taatcccaac ggacattatg
     1681 tagatggtcc ggtattagat acacaatttc gttcatttgt aggcatgcaa cagattcctg
     1741 ttgtttacgg aatgactata ggtgaatatg cccgaatgtt aaatggcgag aaatggcttg
     1801 caaaaaaagc agtatgtaag ttgactgtca tttcttgcaa aaattacaat cacacaactt
     1861 tttattcatt accggttaaa ccttcgccga atctgcctaa tatgactgct atttatttat
     1921 atccgagttt atgtctgttt gaaggtacgg ataaaatcag cttgggcaga ggcactgaca
     1981 aaccatttca gatatatggc agtgatttat tttccgatga catgctatat cagtttaccc
     2041 ctcaaagccg tgaaggtgca aaatcaccgc cattaatgaa taagctttgt tatgggtatg
     2101 atttaagtga acttccaata gatagcctac gcaatcaaaa atttacattg cgttactttc
     2161 tgcaagcgta taaccagatg ccggataaaa ataaattcct gaactcgttt ttcaataagc
     2221 taatgggaag cactgctttt ccgcaacaat tagaaaaagg gatgagtgaa aaagcgatta
     2281 gggcaagctg gcaaactaaa ctcaataaat ttaagcttat ccgcaaaaaa tatttactgt
     2341 ataaagactt tgaataatgc agaataattt tttatcacaa gtaaacatgc ctttttttgg
     2401 catagttttt gtttatgctt acaaaaagct atttttattc agttaaacta ttacgcatgt
     2461 acaaaaaaca tttacttttt ctactcttta ccagcttgtt tgcttggaag agtaacgcac
     2521 aaaatgcaag tgcaaacatc aatgatttga ttcaaaagag ctttacagcg atggcgaatg
     2581 taaaaaccat gacttatcat ttctatgctt cagaacgcat ggatgatggt acgtattcta
     2641 aaagcgatgt ggaattcaaa gttgtggcca gcccattaaa aatatttgcc aatgctcatg
     2701 agccccaatc agcaaaacta agttatgatg cttcaaaatc aaaagatgta aaagtaaaaa
     2761 aaggactttc attaagctta agcccaacga ataaactttt gatgaaaggc gttcacaacc
     2821 caataactcg tgcaggtttc ggtcgaataa aaatgatctt agaaaagagc ttggcacaac
     2881 gcaagggaca agatttaagt aaatatgtaa aacttgcagg cactgtaatg tatgacaaca
     2941 aagaatgttt aaaagtagtt atctcagacc ctgactataa aataatagac tatacggtaa
     3001 aacccggaca aaccaatgtc tggcaaattg gatcagattt ggctatttca gaatacaaaa
     3061 taaaagaatt gaatggcatc ggtgatgatg taaaacctgg tcagaaactg aaaattccat
     3121 catcatatgc taaagtaacg accatttatt ttgacaaagc cactaatctt cctgtttttc
     3181 acagaatgga agatgagaaa ggggtatatg aaacttatga gtttaaaaac ttgaaaaccg
     3241 gcatgacctt tactgatgca gatttccaat tttaacagaa aaaattacaa cataaaaaaa
     3301 ggctgtccga tggacagcct tttttcgttt ttatatcact tttataagtg taacttttct
     3361 ttttttgcca aaagttctgc ttctgtttcg acgtggtttt catcaggaat acaacaatca
     3421 accggacaaa cagcagcaca ttgtggttct tcatgaaaac ctttgcattc tgtacattta
     3481 tccgttacaa taaaatacaa atccatggaa accggatatt gctttgcttc tgcatcaact
     3541 actctcccat ccatcaatgt ataatttcct ttaacagtat ttccatctgc aattcgccat
     3601 tctatgccgc cttcatagat agcattattc ggacattccg gttcacaagc tccgcagtta
     3661 atacattcat ctgttattcg aattgccata gtaatgaaat ttaattacaa atatagagat
     3721 ttgctcctaa gcaattatgt aactattttt acaatagtat taactttgta ttttataaat
     3781 tagttccgga aattgaattt ttatgcattt aaatgaacgt attgccgcat ttgagcaatt
     3841 aggtaagtat attttgtcat acgatgaaga gttgaaaaac gctattcaac aggcttattt
     3901 aaacaataac tggttcacgg aagcaagttg cgaaatgcag ctccaaacta tcgcaactga
     3961 ctatttagat ggggttaagc tgtataactg ggtaaaaaac tatacgcttc tgtatgaaga
     4021 caaattgccc cccagaatag tgggaattgt atgtgccgga aatttaccgc tggtaggttt
     4081 tcacgatatt ttgtctgttt taatttgcgg atataccgca caaatcaaat tatcagaaaa
     4141 agataaagag ctccctttat tccttctaca aaaactgatt gacatccagc ccatgttcgc
     4201 atctttaata aatatagtaa gcaagctgga aaattttgat gcaatcattg ctaccggcac
     4261 caaccaaaca gccgcacatt tcagaactta tttcaacaaa taccaacata ttattcgcaa
     4321 tcaccgcaat ggaatcgcca ttttaacggg aacagaaaca gaagaagatt tgtttaatct
     4381 gggtaaagat gtcttttcat attttggatt aggttgtcgg aatgtttctt tgttattcgt
     4441 gccaaaaaat tatgatttca cttcactgat aaaagcctgg caggctttca aagatgtatt
     4501 gatgaatcac aattcttatc gcaataatct ggattatcag cgaaccattt atctgatgaa
     4561 acaaactccg atgattgata ttgactttgt caatatgacg gaaaacgaca gcatgtattc
     4621 gccggtatct tgccttcatt atacttatta tgatgatatt aatgaagtat atacatacat
     4681 caacaaatac gaagcgcaaa ttcagtgtat tataggaaaa gactatctgg cattcggcaa
     4741 aagtcagttg cccaaactgg atgagtatgc tgacaacaca gacaccttag catttttaac
     4801 tcaattacat ggataactca acgaataaaa tagtatgccc tagttgtgga agtggttcat
     4861 atgaacaatt ttcacgttct caatttcgct gtatgaattg caaaacggta tttgatgata
     4921 cagcatttaa aatggagccg gtagaatatg aattagactt agacagcgac ttgctgaacc
     4981 ttaaagaaac aaaattaaca gaaccggttc ctgtaaataa aagagtaatc aattatttgg
     5041 tagactcatt gagcattgcg ctaattatag tcttttttca atctttcgga atagaaatac
     5101 ttgggaacgt atttcaaatt tatctgttat tttataccta ttttttcttt atggaagcag
     5161 cctttgggca gacgattgga aagtttgtta caagaactaa agttgttgat ttaaatggag
     5221 aaaaaccgac tcttggaaga attgctttac gttcaatttg tcgttttatc ccgtttgatc
     5281 ccattagttt tctgtttcaa gaccgtgtat tttggcatga ttcgctttca aaaacgagag
     5341 tggtggatgc ggcttagttt ttttgttaat caattagaga tgcattattt tgacattata
     5401 taatacaata attttatata tagtatatat tatattttat ttacaaaaca tactttttat
     5461 tatctaataa cttattgtag cttgggcatg tttatcataa aaagttgttt agtacaaatc
     5521 tctgcagata gctaagcaat aacagcgtac acccatatcg ttctatttat catttcttgt
     5581 ttcaattttt gtattaaaag cattgtaaat cctataaatc ctcgcatttc ttgtc
//
LOCUS       DAOQII010000163         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8501_length_5535_cov_4.557482, whole genome shotgun sequence.
ACCESSION   DAOQII010000163 DAOQII010000000
VERSION     DAOQII010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8501_length_5535_cov_4.557482"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLF48_08285"
     CDS             complement(<1..189)
                     /locus_tag="PLF48_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HNF49361.1"
                     /translation="MSDKKSTWDEQYMRRCFQLAELGESYVAPNPKVGAVLVFENRII
                     GEGFHRHFGQAHAEVNCIN"
     gene            228..1079
                     /gene="prmC"
                     /locus_tag="PLF48_08290"
     CDS             228..1079
                     /gene="prmC"
                     /locus_tag="PLF48_08290"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNF49362.1"
                     /translation="MNVLHYRQIIQPLLQEEFDEREAQSLFKYVLEEYYQQRFLNLQQ
                     LQLQDDDLENLNTIFEKVCQQYPVQYIFGEADFYGMRFNVNEHVLIPRQETEELVQLI
                     LAHYKDHKTSLNVLDIGTGSGCIAIALKKLHPAFELTAIDISDEALKVAKENGQLNKT
                     EIVFRQEDILHPSTWLLEKKWNLIVSNPPYIPYHEQDKMSASAILHEPTLALFVENDN
                     ALLFYDLIAKYAQSNLSPNGAIYFELNEYNAHDVAQLLSEKYHFKTEIIKDILGKNRM
                     LKGIPYI"
     gene            1084..1941
                     /locus_tag="PLF48_08295"
     CDS             1084..1941
                     /locus_tag="PLF48_08295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNF49363.1"
                     /translation="MNSLKKIFYLKLPLTLVERIQLFIQFALLKIKVRFFNQPNKIIS
                     LNIAGFKLNAYDYETAQYLLEEVWLNEDYYFKTNKKEPVIFDCGANIGVSVLYFKALY
                     PESTIIAFEPNPNLFALLKKNVQVNQLKDVYIHQVALNAKDEEISFFINENLGSFLSS
                     KFQHRGGNNEIKVKAEQLSGFMNNFANIDLLKMDIEGAEYEVIQELNEKKLLGTVHKY
                     IVEYHYFKDRDQGKIVTFINTFAQNGFAYTTDKPIDKSLSFQDVVFTFTKSEKHILSL
                     EQQTHSFPF"
     gene            complement(1903..3234)
                     /locus_tag="PLF48_08300"
     CDS             complement(1903..3234)
                     /locus_tag="PLF48_08300"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF49364.1"
                     /translation="MKYILKGGLLVNENKITEQDILIENERIAKIDRDITDDHATVVD
                     LNGKHILPGIIDDQVHFREPGLTHKATIFSESRAAVAGGVTSFMEQPNTKPAALTQSL
                     LEEKYRIAADTSWCNYTFFMGVSNDNYDEVMRTNPENVGAIKIFMGSSTGNMLVDDEH
                     ILSKIFANSRSIIVTHCEDEKTILAQTEKYKQLFGENIPMNFHPVIRNDEACFLSSKH
                     AVELAKKHQSRLHIFHVSTEKELQLFDNTIPLKDKKITAEVCAHHLYFDERDYARLGS
                     QIKCNPAVKYEQDKNALFAAVLDNRLDVIATDHAPHTWQEKQQNYFSAPSGVPLVQHS
                     INVMLEFHHQGKISLEKIVEKMCHAPADCFRIKERGYLRDGYYADIAVLDLEKKWTVS
                     KENILYKCSWSPFEQHEFKGAVEQTFVNGHLIYDKGKFASEGKGMRLLFEA"
     gene            complement(3218..4525)
                     /locus_tag="PLF48_08305"
     CDS             complement(3218..4525)
                     /locus_tag="PLF48_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HNF49365.1"
                     /translation="MSTNNKNVAFQITIIGLLFFIFGFITWANSSLIPYFKIACELGE
                     GARSFYVASAFFAAYFFMAIPSSYILKLTGFKNGMALGLFIMAIGAALFIPAANARSY
                     NLFLLGLFIIGTGLALLQTASNPYVTILGPIESAAQRISIMGICNKFAGILAVYVLGS
                     ITLKNADEVKAKLATLGEAEKNQMLDNFILRVKEPYTIIAAALLLLSIIILVIKMPDV
                     NEEENAPETKASAQRNSIFQFPYLLLGALAIFFYVGVEVISYDSFTNFGEFLGYKIDI
                     ARNFPTYTGYALLAGYIFNIIAIPKFISQQKALFYFVILSIFLVLLTVFTSGKTAIIC
                     FMLLGFSNSVMWPAIWPLAIEGLGKFTKIGSALLIMGIVGGAILPPLYGYIAGAIGNK
                     QTAYLMMIPAYLYILYYALSGYKVGKEITNNPKELDLTHEIHS"
     gene            4646..>5535
                     /locus_tag="PLF48_08310"
     CDS             4646..>5535
                     /locus_tag="PLF48_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49366.1"
                     /translation="MSKRFLFVFFLLNMSGIFAQGTYIPMGSPTYHFLERLEIKTGLI
                     NQFHTSNKPYDRKMVIDYINNLDSLSDMDVDDLTKMDKENIDYIWKDNFEFDDSHYEY
                     NTEAKLKFLFTHPAHFLSAKVPHFAVSVDPAIQIKLGGSLVDETGSKFKYVTSKGFEV
                     RGQVDDIFGFYTLFTSEQGTFPNYVNQYAKAHITPPGAGYWKDYKKGGYDYFLAKGYV
                     TISPSKHFHVQFGYDNNFIGDGIRSMFLSDFGANYLFLKLNTNVWKINYQNIFAEMTK
                     FYGHGGDGLLTKKYMAMHHL"
BASE COUNT         1813 a         1018 c          981 g         1723 t
ORIGIN      
        1 gttgatgcag tttacttctg catgtgcctg accaaaatgc cggtgaaagc cctcaccaat
       61 tatccggttt tcaaacacca atacagcacc tactttagga ttaggtgcca cgtagctttc
      121 acccagttcg gccagttgaa aacaacgcct catatattgt tcatcccatg tggatttttt
      181 atcgctcacg agtaaaagat aatttctatt ttcacaaaaa tacaaaattg aacgtactcc
      241 actacagaca aatcattcag cctttgcttc aggaagaatt tgatgaacgc gaagcacaat
      301 cattatttaa gtatgtactg gaagaatatt atcagcaacg ttttttaaac ttgcaacaac
      361 tgcaactaca agatgatgac ttagaaaact taaacactat ttttgaaaaa gtatgccaac
      421 aatatcctgt tcaatacatt ttcggtgaag ctgattttta tggcatgcgt ttcaacgtaa
      481 atgagcatgt actcattccg aggcaggaaa ctgaagagtt ggtacaattg attttagcac
      541 attataaaga ccacaaaacc agcttaaatg tgctggatat cggcaccgga agtggctgta
      601 ttgccattgc attaaaaaag ctgcatcctg catttgaact tactgccata gatatttctg
      661 atgaagcatt aaaagttgca aaagaaaacg gacaattaaa taaaacagaa atcgtgttca
      721 ggcaagaaga tattttgcat ccttcaactt ggttgcttga aaaaaaatgg aatctcattg
      781 tttccaatcc tccttatatt ccctatcatg aacaagataa aatgtcggca tctgccattc
      841 tgcatgaacc cactctagct ttgtttgtgg aaaacgataa tgcattgttg ttttatgatt
      901 taatagcaaa atatgcacag tctaacttaa gcccgaatgg agcaatttat tttgagttga
      961 atgaatataa tgcccatgat gtggctcaat tgttgtctga aaaatatcat tttaaaactg
     1021 aaatcataaa agatattttg ggcaaaaata gaatgctgaa aggaatacct tatatttgaa
     1081 accttgaaca gtttaaaaaa aatattttat ttaaagttac ccttgacctt agttgagcgt
     1141 attcagttat tcatacagtt tgcactactg aaaataaaag ttcgtttttt caatcaaccc
     1201 aataaaataa tttcactcaa tatagccgga ttcaaactaa atgcctacga ttatgaaact
     1261 gctcagtatt tgctggaaga agtttggctg aatgaagatt attattttaa aacgaacaaa
     1321 aaagagcctg taatttttga ttgcggtgcg aatatcggag tatcggtact ttatttcaaa
     1381 gcactatatc cggagagtac cattatcgca tttgaaccca atcccaattt atttgcttta
     1441 ctgaagaaaa atgtgcaggt taatcaactg aaagatgtgt acatacatca agttgcatta
     1501 aatgcaaaag atgaagaaat ctcctttttt atcaatgaga atttaggttc ttttttatct
     1561 tcaaaatttc agcatcgcgg tggtaataat gaaattaagg taaaggcaga acaactttcc
     1621 ggtttcatga ataactttgc taatattgat ttattgaaga tggatattga aggtgcagaa
     1681 tatgaagtca ttcaggaatt aaatgaaaaa aaattattgg gtactgtcca caaatatatt
     1741 gtagaatacc attattttaa agacagagac caagggaaaa tagtaacttt tattaatact
     1801 tttgcgcaaa acggatttgc ctatactaca gacaaaccaa tagacaaatc gctcagtttt
     1861 caggatgtgg tgtttacatt tacaaaatca gaaaagcaca ttttaagcct cgaacaacaa
     1921 acgcattcct ttcccttctg atgcaaattt tcctttatca taaataagat gtccattcac
     1981 aaaggtttgt tctactgctc ctttgaattc atgttgttca aatggcgacc aagagcattt
     2041 gtataaaata ttctctttgc ttacagtcca ttttttttct aaatctaaaa ctgcaatatc
     2101 tgcataatag ccgtctctca aataacctcg ttcttttata cgaaaacaat ctgccggagc
     2161 atggcacatt ttctccacga ttttttctaa gcttatttta ccttgatgat gaaattcaag
     2221 catcacattt atggaatgtt gaaccaaagg cacaccgctc ggtgcactaa aataattttg
     2281 ttgtttctct tgccaggtat gtggcgcatg gtcggtggca atgacatcca aacgattgtc
     2341 caatactgcc gcaaacaatg catttttatc ctgttcatat ttcacggcag gattgcattt
     2401 aatctgactc ccaagtcggg cataatctcg ttcatcaaaa tacaaatggt gggcacaaac
     2461 ttcagccgta atctttttat cttttaaagg gatagtatta tcaaaaagct gaagttcttt
     2521 ttctgttgaa acatgaaata tatgcagtct ggattgatgt ttttttgcta attccaccgc
     2581 atgttttgat gaaagaaaac aggcttcatc attgcgaatt accggatgaa aattcattgg
     2641 aatattttcg ccaaagagct gtttatattt ttcggtttgc gctaagattg ttttttcatc
     2701 ttcgcaatgc gtaacaatga tgctacgtga attggcaaaa atcttggata atatatgttc
     2761 gtcatctacc agcatattcc cggttgacga acccataaat attttgatgg cacccacgtt
     2821 ttccgggtta gtgcgcatca cttcatcata gttgtcattt gaaacaccca tgaaaaaggt
     2881 gtaattacac cacgatgtgt cagcggcaat gcggtatttt tcttctagca gggattgagt
     2941 taatgcggca ggcttagtat tgggctgttc catgaatgat gtaacaccac ctgctacagc
     3001 tgctctcgac tctgaaaaaa tcgtggcttt atgtgttaaa cccggctctc taaaatgcac
     3061 ctgatcgtcg ataatgcccg gtaaaatatg cttaccattt aagtcgacaa cagtagcatg
     3121 atcatccgta atatcgcgat ctattttagc gatgcgctcg ttctcaatta aaatatcctg
     3181 ctcagtaatt ttgttttcgt tgaccagaag gccaccttta agaatgtatt tcatgtgtca
     3241 aatccagttc tttagggtta tttgtaattt ctttccctac cttatatcca ctgagtgcat
     3301 aatacaaaat gtataaataa gctggtatca tcattaaata ggcagtttgc ttattgccaa
     3361 tggcacctgc aatatatccg tataatggcg gcagtatagc accgcctact atgcccataa
     3421 ttaataaagc cgaacctatt tttgtaaatt tccctaagcc ttctatagcc aagggccaaa
     3481 tagcaggcca catcaccgag ttggaaaagc ctaacaacat gaaacagatt atggctgttt
     3541 tacccgaagt aaatacagtc aatagtacta agaaaatact caaaataaca aaataaaaca
     3601 aagccttctg ttgcgaaata aatttcggaa tggctataat attgaatata taacccgcta
     3661 aaagtgcata acccgtgtaa gttggaaaat tgcgggcgat atcaatttta tagcctagaa
     3721 actcaccgaa gttcgtaaaa gaatcataac tgattacttc tacgcccaca taaaagaaaa
     3781 tagccaatgc ccctaacaac aaatagggaa attgaaaaat actatttcgc tgagcagaag
     3841 ctttcgtttc aggagcattt tcctcctcgt tgacatcggg catttttatc actaaaataa
     3901 taatagaaag aagtaacagt gccgctgcga taattgtata aggttctttt acacgcagta
     3961 taaaattatc cagcatttgg tttttttctg cttcgcccaa agtcgccagt ttggctttta
     4021 cttcatctgc atttttcaag gtgatagaac ctaaaacata tactgcaagt attccggcaa
     4081 acttattgca aatacccata atagagatgc gttgcgctgc actttcaata ggtcctaata
     4141 ttgttacata agggttggag gctgtttgta ataaggcaag tccagtgccg atgatgaata
     4201 aacccaataa aaacaagtta tagctacgag cattggcagc aggaatgaat aaggcagcac
     4261 ctattgccat gataaagagg cccaatgcca taccattttt aaaccctgtt aactttaaga
     4321 tataagaaga agggatggcc atgaaaaaat aagctgcaaa aaatgcggat gctacataga
     4381 atgaacgcgc cccctctccc agctcgcagg ctatcttgaa ataaggaatt aatgagctgt
     4441 ttgcccaagt gataaaaccg aaaataaaaa acagtagtcc gataatggta atctgaaagg
     4501 ctacgttttt gttgtttgtg ctcatagaga tgatttattt gtttaatgtg tgcaatcaaa
     4561 gatattcaaa tatagagaaa aatacgtcta tgcaaaccaa tattcttata tttaccgtgc
     4621 attctgttgc agttaactta tccatatgag caaacgattt ctatttgtat tttttttgtt
     4681 gaatatgtcc ggcattttcg cgcaaggcac ttacattcca atgggcagtc ccacttatca
     4741 ttttttggaa agactggaaa taaaaaccgg attaattaat cagtttcaca cctccaataa
     4801 accttacgac cgcaaaatgg tgattgacta tatcaataat ctcgattcat tgagcgatat
     4861 ggatgtggat gacttaacta aaatggataa agaaaatatc gactatatct ggaaagacaa
     4921 ttttgaattt gacgacagcc attacgaata caacacagaa gcaaaattaa aattcttgtt
     4981 tacgcatcct gcgcattttt taagtgccaa agtaccgcat tttgctgttt ccgtagatcc
     5041 tgccatacaa ataaaattag gtggttcttt agtcgatgaa acaggatcaa aattcaaata
     5101 tgtaacctct aaaggttttg aagtgcgtgg gcaagtggat gatattttcg gattttatac
     5161 tttgtttact tctgagcaag gaacatttcc gaattatgtc aatcagtatg ccaaggctca
     5221 tataactcct ccgggtgccg gctattggaa agattataaa aaagggggtt acgattattt
     5281 tttggccaaa ggatatgtga cgatttcacc aagcaagcat tttcatgtac agtttggcta
     5341 cgacaataat tttattggtg atggcattcg ttccatgttt ttatccgatt tcggagcaaa
     5401 ttatttgttc ttaaagctga atacgaatgt atggaaaata aactatcaga atatttttgc
     5461 tgagatgacc aaattttacg gacatggcgg agatggctta ttgacaaaaa aatatatggc
     5521 catgcatcat ctgag
//
LOCUS       DAOQII010000164         5515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8531_length_5515_cov_4.587179, whole genome shotgun sequence.
ACCESSION   DAOQII010000164 DAOQII010000000
VERSION     DAOQII010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8531_length_5515_cov_4.587179"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1356)
                     /locus_tag="PLF48_08315"
     CDS             complement(133..1356)
                     /locus_tag="PLF48_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF49367.1"
                     /translation="MRFCLSYLISVVFVCTTMQLQAETIIRTKKHKTSIPFELYNNIM
                     VVKMDINRSKTLHFIFDSGCRSNIVLHPKWLDSFQIVSDEKVFFSGLGLKDTIETIKA
                     TNCQIQLAALTDDKADIYFLNKDTLLLENYLGTPIDGIFGAELFKKYYIHINYKKRLI
                     ELFENKPSGKIKSDFKKIPLKIKMSKGYYTCIIMNANGELYESELLLDTGANLPLLIK
                     NTSPEQMGVLQYKEIEIGEGLAGPLLSKVGRTKTLFFDTLRFEQPVTCFSETPLSLHE
                     NNDAVLNGNIGNDILSRLDIYFAYPEQSLYYRTTKHTYEPFYFNISNIILLQNKSGNG
                     GFIVKNIIPNSPPSEMGLDKGDEILEINGVKTEKMEMSDALYLLNRKIGKKITVVFRH
                     LNQTKKITYKLESVI"
     gene            1401..3257
                     /locus_tag="PLF48_08320"
     CDS             1401..3257
                     /locus_tag="PLF48_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNF49368.1"
                     /translation="MNYLSVTNLTKYYGERCLFENITFYIDKGQKLALIAKNGTGKSS
                     LLKILEGQEPPESGEYKIHKDINCGFLAQEPEFDEQHTVFDAILYSDNPMLKASLEYE
                     HALAGIGNLEDAIDKMDKLFAWDYEKQVKQILSKFLITDFDQKVASLSGGQKKRLALA
                     RLLLIKPDLLILDEPTNHLDMDMIEWLETELSKQNITLLMITHDRYFLENICTDILEL
                     EDNKLYKHKGNYSVYLENKAIREENARVSVEHAQSVFRKELEWMRRQPKARTTKAKSR
                     IDNFEQVKEKAQQLVQEQQVQLELKMNRLGGKILELHNVSKQYGSKQVLDKFSYKFQR
                     GERVGIVGRNGFGKSTLLNILTGLEKPDSGNIVLGETVVFGYYNQQGLQLKADKRVLE
                     VVKDIAEYLPVNKGQISAAQLLERFLFAPSQQHQYVSTLSGGEKRRLYLLTILMKNPN
                     FLILDEPTNDLDILTLQALEDFLSEYKGCLLIVSHDRYFLDKLTDHIFIIEEEGKIRD
                     YLGNYKEYRAQKKAEEREKKNEEASRLNENKQAKNTDASKKKMTFKERQEFQLLENEL
                     QELADIKTSLTNALSSGVQTHEEITAASLELQRITELLDEKEMRWLELSEYQ"
     gene            complement(3297..4013)
                     /gene="bla"
                     /locus_tag="PLF48_08325"
     CDS             complement(3297..4013)
                     /gene="bla"
                     /locus_tag="PLF48_08325"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaB/IND/MUS family subclass B1
                     metallo-beta-lactamase"
                     /protein_id="HNF49369.1"
                     /translation="MKRILSFFILTLRLTFAFSQTEEKLLIKPLTDSIYIYTTYNFYE
                     NTKLPANGMYIVTEKGVVLFDTPWDTTQFQPLLDSIQLKHHQPVLLCIATHWHSDKTA
                     GLEYYRQLGIKTYTTALTDELSKINDKKRAEFIMTKDTAFRVGNLQFETYYPGEGHTA
                     DNIVIWLNNEKILYAGCLIKGAKSKNLGYLGDANVKAYYQTLKNVAEKYPESNIIVLT
                     HSNWDKGKSLKHSIKLAKKL"
     gene            complement(4021..4659)
                     /locus_tag="PLF48_08330"
     CDS             complement(4021..4659)
                     /locus_tag="PLF48_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNF49370.1"
                     /translation="MEFNKITRGQNRSSNTEESVYAILDAGFLCHIAFQHEGQTMMIP
                     TAYGRKDDALFIHGSKKNFMLNQIINGQTVCVGVTHLDGIVLARTLFDTSANYRSVVL
                     FGKAVQIIDYDERIEALKVITENIIKGRWSEVPIGEENKVDATMVVKIKIDRASAKIR
                     SGGPQGDENKINVVWSGEIPLMLKAGDPVQDLKFGVTLDMTNSVKTFWEEHK"
     gene            complement(4829..>5515)
                     /locus_tag="PLF48_08335"
     CDS             complement(4829..>5515)
                     /locus_tag="PLF48_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF49371.1"
                     /translation="PEKTDFEFEQVFKSWTDKGGEIRRLGKYWINDECLTNQKIAIYG
                     NQTFALVLAQVYNVKLVSPDDKTIAGLDQTWIKRKIEIKTIADITEKCFPLFIKPVIP
                     KSFIASVFTSKTDFEEATKGLDKLEEILTATIVDDIKAEARAYLKNGVIKDIALYEGK
                     ANIIEGELFLRQFAQKNSDKLPSVVVTDLAYSEKLGWFILEFNSCWGAGLNNCKADKV
                     IDCIIEATIN"
BASE COUNT         1771 a          920 c          993 g         1831 t
ORIGIN      
        1 tgtttttttg taaaattgat gttaaatata acaaaatatt gataaataat gataattttt
       61 tataacaact tttaaattgg ttgaattcaa gtaataaatg taattttgtt aaagaatagg
      121 tactcgttgt tatcaaataa cgctttccag tttatatgta attttcttgg tttgatttaa
      181 atgtcgaaaa acaacggtta tttttttccc gattttgcgg tttaataaat ataaagcatc
      241 cgacatttcc attttttcag ttttcactcc gttgatttcc aaaatttcat ctcctttatc
      301 taaacccatt tctgaaggtg gagaattcgg gataatattt tttacgatga agccaccatt
      361 tccagatttg ttttgcaaga gaatgatgtt tgaaatattg aaataaaaag gttcataggt
      421 gtgtttggtc gttctgtaat ataagctttg ctcgggatat gcaaaataaa tatccaacct
      481 gctcaatata tcattgccaa tgtttccatt tagtacagcg tcattatttt catgcaagga
      541 aaggggtgtt tcgctaaaac acgttaccgg ctgttcgaat cgcaatgtgt caaaaaataa
      601 cgtttttgtt ctgcctactt tagataatag tggtccggct aaaccttcac caatttcaat
      661 ttctttgtat tgcaaaactc ccatttgctc tggtgacgta ttttttatta aaaggggcaa
      721 gtttgctccc gtatccaaca gaagttcact ttcataaagt tcgccatttg cattcatgat
      781 gatacaagta taataccctt tgctcatttt aattttgagc ggtatttttt tgaaatcaga
      841 tttaattttt ccgctaggtt tgttttcaaa taattctata aggcgtttct tatagttaat
      901 gtgaatgtaa tattttttaa aaagttcggc accaaaaata ccgtcaatgg gtgtgcccag
      961 gtaattttcg agaagaagcg tgtctttgtt taagaaataa atatcagcct tgtcgtcagt
     1021 aagtgcagct aattgtattt gacagtttgt tgcctttatc gtttctatcg tgtcttttaa
     1081 ccccaaacct gaaaaaaaca ctttttcatc cgaaacaatt tgaaaagaat ctaaccactt
     1141 ggggtgcaaa acaatattag accgacagcc agaatcaaag ataaaatgca atgtcttact
     1201 ccggttgatg tccattttta ctaccataat attattatag agttcaaatg gtatggacgt
     1261 cttgtgtttt ttagttcgaa taattgtttc tgcttgcaac tgcattgttg tacaaacaaa
     1321 aacaacgcta atcagataag ataaacaaaa tctcatttta taaatgtact aaaaagcgct
     1381 aagtaattta cttttgcagg atgaactatt tatcagtaac caatttgacg aaatattacg
     1441 gcgagcgttg cttgtttgaa aatattactt tttatataga taaaggacaa aaactggcac
     1501 tgattgctaa aaatggcacc ggtaaaagtt cattattaaa aattctggaa ggccaagaac
     1561 cacctgaaag cggggaatat aaaatccata aggatattaa ttgcggtttt ttagcccagg
     1621 aacccgaatt tgatgaacaa cataccgtat ttgatgccat tttatacagc gataatccaa
     1681 tgctaaaggc aagtttggaa tacgagcatg cccttgcagg tatcggtaat ttggaagatg
     1741 ccatagataa aatggataag ctttttgcct gggattatga aaaacaagta aagcaaatct
     1801 tgtctaaatt tttaattacc gatttcgatc aaaaggtagc ttcattatca ggaggacaaa
     1861 agaaaagact ggcgcttgcg cgtttattat taatcaaacc ggacttgctg attttagatg
     1921 agccgactaa ccacttggat atggacatga tagagtggtt ggaaacagag ctttcaaaac
     1981 aaaatataac gcttctgatg ataacgcacg atcgttattt tttggaaaat atttgcacgg
     2041 atattctaga actcgaagac aataaattgt ataaacacaa gggaaattat tctgtctatc
     2101 ttgaaaataa ggctatccgg gaagaaaatg caagagtaag tgtggaacat gcccagagtg
     2161 tatttagaaa agaattggaa tggatgcgca ggcagcctaa agccaggacc acgaaggcaa
     2221 aatcaagaat agataatttt gagcaagtaa aagagaaagc acaacagctt gttcaggaac
     2281 agcaagtgca actggaacta aaaatgaacc gcttaggtgg caaaatcctt gaactgcata
     2341 atgtatccaa acaatatgga tcgaaacagg tactcgataa gttttcttat aaatttcagc
     2401 gaggtgaacg cgtgggtatc gtgggtagaa atggatttgg aaaatctact ttattgaata
     2461 ttcttaccgg attggaaaag ccggattccg gcaatatcgt attaggcgaa actgtcgtat
     2521 ttggttatta caatcagcaa ggcttacaac tgaaagcaga taagcgtgta ttggaagtag
     2581 taaaggatat tgcagaatac ctgcccgtaa ataaagggca aatttcagca gcacaattac
     2641 tggagcgttt tttatttgct ccgtcacaac aacatcagta cgtatctaca ttaagtggtg
     2701 gcgaaaaaag aagattgtat ttgctgacca tcttgatgaa aaatccgaat tttttgattt
     2761 tggatgaacc taccaatgat ttggatatat tgacgcttca ggctttggaa gattttcttt
     2821 ccgaatataa aggttgtttg ctcattgtat cgcacgacag atattttttg gataagctga
     2881 cggatcatat tttcattatt gaagaagaag gaaaaatcag agattattta ggaaattaca
     2941 aagaatacag agcacagaaa aaggcagaag aaagagagaa aaaaaatgaa gaagccagca
     3001 ggttaaatga aaataagcaa gctaaaaata cagatgcgtc taagaaaaaa atgactttta
     3061 aagaaagaca agaatttcag ctattagaaa atgagttaca ggaattagcc gatataaaaa
     3121 caagcttgac aaatgccctg agcagtggag tacaaacgca tgaagaaata acagcagcct
     3181 ctttagaatt acaacgcata acagagttat tagatgaaaa agaaatgcgc tggctggagc
     3241 ttagcgagta tcaataaaat taaacaaaaa aacaggtgca gaaaagaggg tggtttttat
     3301 aatttttttg ctaatttgat agagtgtttc agtgatttgc ctttatccca attgctgtgt
     3361 gtaagtacga taatattgct ttccggatat ttttccgcta cattttttaa tgtctgataa
     3421 taagctttca cattagcatc gcccaaataa cccaggtttt ttgatttggc tccttttatg
     3481 agacaaccgg catatagtat tttctcatta ttcagccata tcacgatatt atcagccgta
     3541 tgtccttcgc cgggataata ggtttcaaat tgtaaatttc ccactctaaa cgccgtgtct
     3601 tttgtcataa taaactcagc acgttttttg tcgtttattt tggataattc atcagttaat
     3661 gccgttgtat aggttttgat tccaagttgt cggtagtatt ccaaacctgc agttttatcg
     3721 ctatgccaat gcgtggcaat acatagcaat acaggttgat gatgtttgag ttgtatactg
     3781 tcgagtaacg gctgaaactg tgtggtatcc caaggggtat cgaaaagtac tacgcctttt
     3841 tccgtaacaa tatacatgcc attggccggt agtttggtgt tttcgtaaaa attgtatgta
     3901 gtataaatat atatactatc agtaagtggc ttaataagca acttttcttc cgtttgacta
     3961 aatgcgaatg taagtcgaag tgttaagata aaaaacgaga gtatcctttt catggcttat
     4021 ttatttatgt tcttcccaaa atgtttttac actgttggtc atgtcaagtg tgacaccgaa
     4081 ttttaaatcc tgaacaggat cacctgcttt tagcataagt ggaatttctc ccgaccaaac
     4141 tacatttatt ttattttcat caccttgcgg tccaccactc ctaattttcg ccgatgccct
     4201 gtctatcttt attttcacca ccatggtagc atctacttta ttttcttcac ctatgggaac
     4261 ttcgctccat ctgcccttga taatattttc ggtgatgact ttcaatgctt cgattcgttc
     4321 gtcataatca atgatttgta cggctttacc aaaaagcaca accgatctgt aattggcaga
     4381 agtatcaaat aaagtacgcg ctaatacgat gccatctaaa tgcgtaactc caacacaaac
     4441 tgtttgtccg ttgatgattt gattcagcat aaaatttttt ttagaaccat gtatgaaaag
     4501 tgcatcgtct ttgcggccat aagctgtcgg tatcatcatt gtttgccctt catgctgaaa
     4561 agcaatatgg cataaaaagc cggcatccag aatagcataa accgactctt cggtattaga
     4621 acttctgttt tgacctctgg ttattttgtt aaattccatt gtaataataa atttgattat
     4681 tatgcagtat agcaaaagtt gaggtgaaat tacatgttag cgttgaacta tcaaaagttg
     4741 ttgcaggttt ttatcatagt aggatgcata acctaaatga tggaggaaaa aatatactat
     4801 accgatttta cggccagtta ttatttgttt agtttattgt cgcttcaata atgcaatcaa
     4861 ttactttatc tgccttacaa ttatttagtc ctgcccccca acaagaatta aattcaagaa
     4921 taaaccatcc aagtttttca gaataggcta agtccgttac aacgacactt ggtagtttgt
     4981 ctgagttttt ttgtgcaaat tgtcttaaaa ataattcacc ctcaattata tttgctttgc
     5041 cttcatataa agcaatatct tttataactc cgtttttcag ataggctctt gcttctgctt
     5101 taatgtcgtc aacaatagta gcggttaata tttcttccaa tttatctaat ccttttgtcg
     5161 cttcttcaaa gtctgttttt gatgtaaaaa cagaggcaat aaaagatttt ggaataacag
     5221 gttttatgaa taagggaaaa cacttttctg ttatgtcagc aatcgtttta atttctattt
     5281 ttcttttaat ccaggtttgg tcaagcccgg caatagtctt atcgtctggt gatactaatt
     5341 ttacattgta aacttgagct aatactaaag caaaagtttg atttccgtaa atcgctattt
     5401 tttgatttgt caaacattca tcattaatcc agtatttccc cagtctcctt atttctccac
     5461 ctttatctgt ccaggattta aaaacttgtt caaattcaaa atctgttttt tcagg
//
LOCUS       DAOQII010000165         5506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8550_length_5506_cov_4.996881, whole genome shotgun sequence.
ACCESSION   DAOQII010000165 DAOQII010000000
VERSION     DAOQII010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8550_length_5506_cov_4.996881"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLF48_08340"
     CDS             <1..523
                     /locus_tag="PLF48_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNF49372.1"
                     /translation="AEDKDFEMNRFLKKETQIMVATTVIEVGVNVPNASVMLIENAER
                     FGLSQLHQLRGRVGRGADQSYCILMTSQKLTTEGKTRINTMCETNDGFKISEVDLQLR
                     GPGNMEGTQQSGNIDLRIANLATDGPIIEEARNCALSVLESDPDLSLPEHAQMKAYYL
                     QQKAQSGHWAKIS"
     gene            573..1253
                     /locus_tag="PLF48_08345"
     CDS             573..1253
                     /locus_tag="PLF48_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49373.1"
                     /translation="MNKIILFSIISLLIFSACNITKKTTPLQCASDTKYVEGKLKNLT
                     GLDGCGWVIALDDSTVLEPVNLADFTIQKSEGMPVQCCYKEVSAASICMVGKTVQLAD
                     IRKTTPNINCDSAICTMDYRTISLKINYVSVPPTDNRITSFKTINTATGAVINEKTNI
                     SVADQDLYALYGYPVVSDGQIPQGTAEMDLTFIGYRNGTEAVRTTIQAGHDCCHIFKV
                     SGVSEVNL"
     gene            complement(1290..1706)
                     /locus_tag="PLF48_08350"
     CDS             complement(1290..1706)
                     /locus_tag="PLF48_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNF49374.1"
                     /translation="MKTLLLTAALAGMLCISYAGGKPKNKSPLTGTWSFTNASKEVDF
                     AESKIFVAADKEQFIFNADFTFSHIFLSKDNQTIKSLKGTFILDGNKISMHYADVDFT
                     LNTQYFFIGNDLVFGSAFSHFIFTKDAGNNLEMAEK"
     gene            complement(1858..2271)
                     /locus_tag="PLF48_08355"
     CDS             complement(1858..2271)
                     /locus_tag="PLF48_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNF49375.1"
                     /translation="MIQRIQTVYLFLAALSAILFIVIPFGQVKTDTGVETWHIYQVMP
                     IMIAAIITALSSLVTIFLFGNRKLQLKFVLLDMFFGIVLLGLFIYGVTQHIGIQHYVF
                     GIGAIFPLLIFLFLFLARISINKDEKLVRSMDRLR"
     gene            2355..3095
                     /gene="truA"
                     /locus_tag="PLF48_08360"
     CDS             2355..3095
                     /gene="truA"
                     /locus_tag="PLF48_08360"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNF49376.1"
                     /translation="MRYFLKLAYHGKNYCGWQRQENAIAVQQVLEEKLQLVIGYPADI
                     TGCGRTDAGVHAHQYFAHFDVRDAISDTELFIYKFNALLPDSIAIYACFPVVDDLHAR
                     FSASSRSYKYFMHYGKNPFRDDFSFSLQNKRPDIQLMNQCCRHLLGEYDFSTFEKKGS
                     DNKTSVCNLTDAHFTETEDGIVFTITANRFLRNMVRRITGAMLMVGLNQLKSEELITL
                     VRNQQTLNVKIAVPAKGLFLWEIIYPNF"
     gene            3101..4870
                     /locus_tag="PLF48_08365"
     CDS             3101..4870
                     /locus_tag="PLF48_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF49377.1"
                     /translation="MQTANQNKSGDWNTLKRVIRLAIPFKWLFAWTFFLGIAVSIITP
                     IRPYLIQLTVDNNIIKQHGKGLTMMSLLLIGTLLIEFIIKYLFSLYTTQLGQNVMLKL
                     RTDLFRHVLNMRLQYFDKTPVGIITTRTINDIEAINNIFSEGLITIVADVLTLIFVVA
                     FMLYINWKIALVCLTTFPIVILATYIFKEKVRVSFNEVRDKVSLLNAFVQEHISGMRI
                     VQIFNAQEREYKKFETLNKEHYKANDKSVLYYSIFFPVVEVILALAISLLIWFGLRQV
                     MAHVAGIGVFASFILYLNMAFRPLRMLADKFNTLQMGIIAAERVFKLMDMDDRISDKG
                     KIVAKDIKGDIEFKQVWFAYNDDNYVLKDISFSVKAGETLALVGATGSGKSSIINILN
                     RFYEINKGEITIDGINTKDYRLENLRALIGTVLQDVFLFSGTIAENISLKNPSISLYE
                     IIEASKLVGAHDFIMDLPGGYDYNVMERGATLSVGQCQLISFIRALIYNPHILVLDEA
                     TSSIDTESEILIQQAIEKLVKGRTSIVIAHRLSTIQNADKIILLEKGVIKECGTHEEL
                     LKIDNGAYRYLYEMQFNSHHHFI"
     gene            complement(4858..5106)
                     /locus_tag="PLF48_08370"
     CDS             complement(4858..5106)
                     /locus_tag="PLF48_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49378.1"
                     /translation="MVYFLNSSGKPATINLTDKNIKNASYTVLETTKLYAAISKTYFE
                     AKHPEMNNIPVTEKRDIKVENNQIKLAPYAFGYVAYIK"
BASE COUNT         1782 a          971 c         1056 g         1697 t
ORIGIN      
        1 agcggaagat aaagattttg agatgaaccg ttttttgaaa aaagaaacgc aaatcatggt
       61 agccaccacg gtgatagagg tgggagtaaa cgtgccgaat gccagtgtga tgctgataga
      121 aaatgcagag cgattcggct tatcacaatt gcaccagcta cgcggcagag taggacgggg
      181 agccgaccaa tcttattgta ttttgatgac ttcacagaag ctgacaacag aaggaaagac
      241 acgtatcaat acgatgtgtg aaaccaatga cggctttaag atctcggaag tagatttgca
      301 acttcggggt ccgggaaata tggaaggcac tcaacaaagc ggcaatattg atttgcgtat
      361 tgccaactta gccactgatg gccctattat cgaagaagcc agaaattgtg cattaagtgt
      421 attagaaagc gaccctgatt tatcactgcc tgaacacgca caaatgaaag cttattactt
      481 acagcagaaa gcacaaagcg gacattgggc gaaaattagt taattccatt taaattcacc
      541 ttgtgtttgt ggtttttatg taattttgaa gtatgaataa aataatctta ttttctatca
      601 tctcactttt aattttctca gcttgtaata taactaagaa aaccacacca ctacaatgtg
      661 cttccgatac aaaatatgtg gaaggcaaac ttaaaaatct taccggatta gatggttgcg
      721 gatgggtaat tgctttggac gacagtactg ttttagaacc tgttaattta gcagatttta
      781 ctatacaaaa atcagagggc atgcccgtac aatgctgtta taaagaagtg tccgcagcta
      841 gtatctgtat ggttggaaaa acggtacaac tggctgacat tcgtaaaaca acaccgaata
      901 ttaactgcga cagtgctatc tgcaccatgg attacagaac tatcagttta aaaattaatt
      961 atgtatcggt gccgcctaca gacaaccgaa tcacttcttt caaaacaatc aatactgcta
     1021 ctggtgcagt aattaatgag aaaacaaaca tatccgtagc tgatcaagat ttatatgcac
     1081 tatatggtta tccggtagtc agtgatgggc agataccgca aggtactgcc gaaatggatc
     1141 ttacttttat cggatataga aatggcactg aagccgtgcg cactactata caagcagggc
     1201 acgactgttg ccatattttt aaggtaagcg gtgtaagcga agtgaatctt tgataaagaa
     1261 attctagatc aactatacca ttgcaagctt tacttttctg ccatttccag attattcccg
     1321 gcatcttttg taaaaataaa atggctgaat gcacttccaa atacaaggtc atttccaata
     1381 aagaaatact gagtattcaa cgtaaaatct acatcagcat agtgcatact gattttattt
     1441 ccatccagta taaaggtacc tttcaatgat tttatagttt gattgtcttt actcaggaaa
     1501 atatgcgaaa aggtaaagtc agcattaaaa ataaactgtt ccttatcagc agctacaaag
     1561 attttacttt cagcaaagtc aacctctttt gaagcatttg taaaactcca agttcccgta
     1621 agcggacttt tgtttttggg cttaccgcct gcgtatgata tgcagagcat accggcaagg
     1681 gcagcagtta ataataatgt tttcatagta gtttgtttta ttatttcaaa cttagtatga
     1741 aagacagaaa gatgacggat aaatgattaa atggtcatat tttatttgtg aatggtataa
     1801 ctcagaaaga aaacgaccga ataatatctg aaaataaatg aaagaatgta tattttatca
     1861 gcgcaatctg tccatggaac gtaccaactt ctcatcttta ttgatagaaa ttctcgcaag
     1921 aaataaaaac aaaaatatga gcagagggaa tatagcccct atgccgaaaa cataatgttg
     1981 aattccgata tgctgcgtaa ctccgtatat aaaaagtccg agcaagacaa taccaaaaaa
     2041 catatccagc aagacaaact ttaattgcaa ttttctgttg ccaaataaaa agatggtaac
     2101 caaactggaa agtgcagtaa taatagcagc aatcataatt ggcatgactt gataaatatg
     2161 ccaggtttct acacctgtat cggtttttac ttgcccaaaa ggaatgacaa taaacaaaat
     2221 agctgaaagt gctgccaaaa acaaatatac ggtttgtatg cgttggatca ttttttctaa
     2281 aatttaatag gacaaaatta aactttaact ctcaatatca aaaacgaaat ctttgtatct
     2341 tcatcgttta caagatgcgg tattttctca aattagctta tcacggaaaa aattattgtg
     2401 gctggcagcg gcaggaaaat gcgattgcgg tgcagcaagt gctggaagaa aaactgcaat
     2461 tagttattgg ctatccagct gatatcacgg gctgcgggcg cactgatgcc ggcgttcatg
     2521 cccatcagta ttttgcccac tttgatgtac gagatgcaat ttcggatacg gaattattta
     2581 tctacaaatt caatgccctt ctgccagata gtattgcaat ttatgcatgc tttccggtgg
     2641 ttgatgattt gcatgcacgt ttctccgcca gcagtcgttc gtacaaatat tttatgcatt
     2701 atggcaaaaa cccttttcgg gatgattttt ctttttcttt gcaaaacaaa cgccctgata
     2761 tccaactgat gaatcaatgt tgcaggcatt tgttgggtga atacgatttt tctacttttg
     2821 aaaaaaaagg cagcgataat aaaacgtctg tgtgtaatct gacagatgct cattttactg
     2881 aaacggaaga tggcatagtg tttacaatta ccgctaatcg ttttttgcga aatatggtgc
     2941 gccgtataac aggggctatg ctcatggtcg gtcttaatca gcttaagtcc gaagaactga
     3001 ttacactggt aagaaatcaa caaacattaa atgtaaaaat tgcagttccg gcaaaaggat
     3061 tatttttgtg ggaaattatt tatcccaact tttgagcctg atgcaaacag caaaccaaaa
     3121 taaatccggt gactggaata cgctcaagcg agttatccgt ttggcaattc cattcaagtg
     3181 gttatttgca tggacatttt ttttgggaat tgccgtttcg ataattacac caatacgtcc
     3241 gtaccttata cagctcactg ttgataataa tattatcaaa cagcacggaa aaggactgac
     3301 aatgatgagt ctgttgctca ttggaaccct gcttatcgag tttatcataa aatatttatt
     3361 cagcttatat acgacacaac ttggacaaaa tgtgatgcta aaactacgta ctgatttatt
     3421 tcggcatgtg ctgaatatgc gtctgcaata ttttgataaa acacccgtgg gcattatcac
     3481 aactcgtact atcaatgaca ttgaagccat caacaatatc ttttcggaag gattaatcac
     3541 tatcgttgcg gatgtgctga cattgatttt tgtcgttgct tttatgttgt atatcaactg
     3601 gaagattgct ttagtctgct taactacctt tcctattgtc attttggcta cttatatttt
     3661 taaagaaaaa gtaagggtaa gttttaatga ggtacgagat aaagtttcgt tgctcaatgc
     3721 ctttgtgcaa gagcatattt ccggtatgcg cattgttcag atttttaatg cacaagaacg
     3781 ggagtataaa aaatttgaaa cattaaacaa agaacattac aaagccaatg acaaatcagt
     3841 gctgtattat tccatatttt ttccggtagt agaagtcata ctggcattgg caatatctct
     3901 cttaatctgg tttggtttgc ggcaagtaat ggcacatgta gccggaatcg gtgtttttgc
     3961 ttcctttatt ctctatctca atatggcatt ccgaccattg cgtatgctcg ccgataagtt
     4021 taatacattg caaatgggca ttattgccgc agagcgtgtg ttcaagctga tggatatgga
     4081 cgataggatt tcagataagg gtaagatagt ggcaaaagat attaaaggtg atattgaatt
     4141 taaacaagtt tggtttgctt acaatgatga taattatgta ttgaaagata tttcttttag
     4201 cgtaaaagca ggggaaacct tagcactggt aggtgcgaca ggttccggaa aatcatctat
     4261 tatcaatata ctcaatcgct tttatgaaat taataaaggc gaaattacaa tcgacggcat
     4321 taatacgaaa gactatcgtc tcgaaaatct gcgtgcttta attggtacag tcttgcaaga
     4381 tgttttttta ttttccggta cgattgcaga aaatatatcg ctcaaaaatc cgtccatttc
     4441 cctatatgaa attattgaag cttccaaatt agtaggtgcg catgatttta tcatggattt
     4501 gcccggtggt tacgattaca acgtgatgga gcggggggca acattatctg ttgggcaatg
     4561 tcagctaatc tcatttattc gtgcattaat ttataacccg catattttag tgttggatga
     4621 ggcaacatca tcaattgata ccgaatctga gattttaata cagcaggcta tcgaaaaatt
     4681 ggtaaagggc agaacttcca ttgtaattgc tcatcgactt tctactatac aaaatgcgga
     4741 taaaattatc ttgttagaaa aaggtgttat aaaggaatgc gggacgcacg aagagttact
     4801 caaaatcgat aatggtgcct ataggtattt gtatgaaatg cagttcaaca gccaccatca
     4861 ttttatataa gcaacataac caaatgcata aggagccagc tttatctgat tgttttcaac
     4921 tttgatatct cttttttcag taaccggaat attgttcatt tccggatgtt tggcttcaaa
     4981 atatgttttt gaaatagccg cataaagctt agttgtttcc aaaaccgtat aacttgcatt
     5041 ctttatattt ttatcagtca gatttatcgt tgccggtttc ccgctgctgt ttagaaaata
     5101 aaccaaggtt cttttggagg atttatcata aaatgattta acaataattc cctcttgcgg
     5161 atttttctca aaattatcac ccagtctgac agactcaggt tgcagggtgg tgcttaagta
     5221 tttgaaagca taatattcta tcgtggttct gttatttcct attttttcta atggtgtatt
     5281 cttgctgggc caatcattta caaacccaac gatatcttca cttaagaaac ttaaatagga
     5341 gaatagtttg gtcagtagtc aagttaattc agcaacaatg ctacctgaaa tcaaaaatac
     5401 tcctccaatt cttaatatcc ccaggcctaa accaaaacta aagcttgaag taaagtaata
     5461 gttaactcca ggataaatga aaaagaaaca aatcttaatt cataaa
//
LOCUS       DAOQII010000166         5474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8607_length_5474_cov_3.670788, whole genome shotgun sequence.
ACCESSION   DAOQII010000166 DAOQII010000000
VERSION     DAOQII010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8607_length_5474_cov_3.670788"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLF48_08375"
     CDS             complement(<1..182)
                     /locus_tag="PLF48_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF49379.1"
                     /translation="MTFNNLLWQVENDILLLTINRPDKLNALNGETVLEIQKAFAHAR
                     TEKLNGIILTGAGEKA"
     gene            complement(189..1559)
                     /locus_tag="PLF48_08380"
     CDS             complement(189..1559)
                     /locus_tag="PLF48_08380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNF49380.1"
                     /translation="MKELLLLSGLGIFALVAELISVRKLIFPVVLIGLLVNIYFCVWD
                     FNQPQNVYNMLFVDKFSLMFTGLTSIVAFFWFIFARSHFGDTPHESDFFALTLFALVG
                     IFVLTSYSHFVMLFLGIEILSIPVYVLAGSDKWNVHSNESAFKYFILGAFASAFLLLG
                     IVFIYGAMGSFDVSRITSVPFLKIGLSKQLLYVGMLLILFAMSFKVAAVPFHFWSPDV
                     YTGAPTFVTAFMSTLVKIGAIAAFLRIISVFVSLNFFSFNKILLIIGILSIIVGNILA
                     VSQANIKRMLAYSGIGHVGFMILAICMLSPSTVYVVWYYMFAYVFANIGAFWVLSKLD
                     NNNDLSAFNGLVRRNPLLAGTLTICMLSMAGIPPLAGFFAKYFVLINLIGSQYVWIAI
                     IAILASLIGVYYYFKVIIAVFAGKETQTSKIKVPLLDRIALLLISIVLIVVGIFPDFF
                     FKLFFK"
     gene            complement(1581..3071)
                     /locus_tag="PLF48_08385"
     CDS             complement(1581..3071)
                     /locus_tag="PLF48_08385"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNF49381.1"
                     /translation="MLILLLLFIPVLFVPLIWLMNDGNAKRIALLGALVEMCVGFYAF
                     YMFSLHPDCACFKVDYPWIFSLNARFQFAMDGISAVMVLLTTLMVPFIIQSTLTESIK
                     RTKAFLSLILLMQFALVGVFLAKDALLFYFFWELALLPVFLLSLIWGGPDRKQITFKF
                     FVYTLFGSLFMLLAIIYIWLRTSGGTEGVTQSFNILAFYDAAQRLSANEQLFVFLAFY
                     LAFAIKIPIFPFHSWQADTYVNAPNQGTMLLSGLMSKMGTFALIIWLIPCAPLGWQLA
                     QKYVIIISVVSLLYGAFLAIAQPHFKRLLAYSSLSHVGLIAAGIFTMNLQGLQGGLYQ
                     MFSHGIYVIGLFFIVDILFKKVKTDSLFELGGIRNVSKVFAVYFLIILLGSVAMPFTN
                     GFVGEFLLLNGLFQYNYILVALAGLTVILGAVYMLRAYQRMMHGKTNDLTALFGDIEL
                     NDKILLACFSGLIIFFGLFPDIIFSVSDEPLKALAEHVQQVILMHK"
     gene            complement(3125..5020)
                     /gene="nuoL"
                     /locus_tag="PLF48_08390"
     CDS             complement(3125..5020)
                     /gene="nuoL"
                     /locus_tag="PLF48_08390"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNF49382.1"
                     /translation="MSLLMLIPLFPLISFLIIAVLGNKLPKSVTSLLAPGSILLSFIV
                     AVIVFFKILHTGESLTAHILPWINVGTFKSEIALTADSLSILFTLVITGVGFLIHVYS
                     VGYMHDEPDYNRYFSYLNLFVFFMLLLVLGNNFLLMFVGWEGVGLCSYLLIGFWFSNI
                     DYAGAAKKAFIMNRIGDLGFILGIILIFGVFGTIDYHTVFNLAGDVKVNGQLITWITL
                     LLFIGAMGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMVARCNVLFTLSHF
                     TMTFISIIGIATALLSATIAMRQNDIKKVLAYSTVSQLGLIFTALGCDAFSAGVFHVV
                     THAFFKALLFLGAGSVIHGMSGDQDIRNMGGLKDKMRITFITFLIGTVAISGIPPFAG
                     FFSKDQMLAEIFEHSKILFVLALFASLMTAFYMFRLLFLTFYGSFRGTAEQEHHIHES
                     PATMTFPLIVLAILSTIGGFLGFPHGIGHALGIHHWIDGFLSSSVTVTENDVNLNREL
                     MLIGITIVTILAVILLARNIFVTNKKVPVADGEPASLWREIVYHKFYVDEIYEAIIVK
                     PMNKFASLLNEWIEKSGIDTVVNGIGSAVLSWSSMMRYTQNGNLGFYVIAMVLGVAVF
                     LLLSVLI"
     gene            complement(5027..>5474)
                     /locus_tag="PLF48_08395"
     CDS             complement(5027..>5474)
                     /locus_tag="PLF48_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49383.1"
                     /translation="LQDVNDTDVNVNVDKGVVLISLSDGMLYKSGSYEILPRAGEILG
                     KIAKVINDYSTFDVLIEGNTDNVPIKNSCMLDNWDLSVKRATSIARALQLQYAVNPAR
                     IIAGGRSEFYPKVANDTPANKAINRRTEIIIMPKLDEFFKLLEIKK"
BASE COUNT         1985 a         1089 c          925 g         1475 t
ORIGIN      
        1 aatgctttct ctccagctcc tgttaatata ataccattta atttttcagt tctggcatgt
       61 gcaaaggctt tttgtatttc taatacagtt tcgccattta gggcattcag tttatccgga
      121 cgatttatgg taagcaatag aatatcgttt tctacttgcc aaagtaaatt attaaatgtc
      181 ataaaaaatt atttgaaaaa aagtttgaaa aagaaatcag gaaaaatgcc gaccacaatc
      241 aatacaatag aaattagcaa caaagcaata cgatctaaga gcgggacttt tattttactg
      301 gtttgagttt ctttacctgc gaaaacggca ataatcactt tgaaataata atataccccg
      361 attagagatg ctaaaattgc aataatagca atccatacat attgactgcc tattaaattg
      421 ataagcacaa aatatttggc aaaaaatcct gctaaaggcg gaatacctgc catagacaac
      481 atgcagatag taagcgtgcc tgctaataaa gggttgcgtc tgaccagtcc attaaatgct
      541 gacaagtcat tattattatc tagttttgac aacacccaga atgcgccgat atttgcaaaa
      601 acataagcaa acatatagta ccatactaca taaacagtag aagggctaag catacatata
      661 gccagtatca taaaaccaac atgcccgata ccggaatagg ccagcatgcg ttttatattg
      721 gcttgcgaaa cagccagtat atttcctact atgatagaca gtatgcctat tatcaataag
      781 attttattga aagagaagaa attcagagaa acaaaaactg atatgatacg caaaaatgca
      841 gcgattgctc caatttttac cagcgtagac ataaatgccg taacaaaggt tggtgcccct
      901 gtgtagacat ccggcgacca aaaatggaaa ggcaccgcag ctactttaaa tgacatggca
      961 aataagataa gcaacatacc cacatacagc aattgtttgc ttaagccaat tttcaaaaaa
     1021 ggtacgcttg taattctgct tacgtcgaaa gaacccatgg caccatagat aaatacgata
     1081 cctaataaaa gaaaggcact ggcaaaagct cctaagataa aatacttgaa tgctgattcg
     1141 ttggagtgta cattccattt atcgctgccg gctaaaacat aaacaggtat ggataaaatt
     1201 tcaataccga gaaacaacat gacaaaatga gaataagatg tcagaacaaa aatgccgact
     1261 aatgcaaata aagtcaatgc aaagaagtct gactcatgtg gtgtatcacc aaaatgagag
     1321 cgggcgaaaa taaaccagaa aaatgccact atggatgtca gtccggtaaa catcagtgaa
     1381 aatttatcga caaacaacat attgtaaaca ttttgcggtt ggttgaagtc ccaaacacag
     1441 aaatagatat taaccaacag accaatgagg acaacaggaa atatcagctt acgcacggaa
     1501 attaattcag ccaccaaagc aaaaattccc aatccggata acaataataa ttctttcata
     1561 atttctactc caggtatgtt ttatttatgc attaaaataa cttgttgaac atgctctgcc
     1621 aatgctttca acggctcatc actgacactg aatattatat cgggaaacaa tccgaaaaat
     1681 attatcaaac ccgaaaaaca agccagcaga attttatcat ttaattcaat atcaccaaaa
     1741 agcgcagtaa gatcatttgt tttgccatgc atcatccgct gataagcacg cagcatatat
     1801 acagctccca aaatcacggt taatcctgcc aaagcgacta atatatagtt gtactgaaat
     1861 aatccgttca gcaataaaaa ctctccgaca aagccattag taaacggcat ggctacactt
     1921 cccaataaga tgataagaaa ataaactgca aatacttttg aaacattacg aatgccgcct
     1981 aattcaaata aactgtctgt tttcactttt ttaaacaaga tatctacgat aaaaaacaaa
     2041 ccgatgacat aaataccgtg agaaaacatc tgatataaac caccttgtaa gccttgcaaa
     2101 ttcatggtaa aaatacctgc tgcaatcaat ccgacatgtg ataaggagga gtatgccaat
     2161 aatcgtttga aatgcggctg tgcaattgcc aaaaatgcgc catacagcaa actgactaca
     2221 ctgatgatta ttacatattt ctgagcgagt tgccagccta atggagcaca tggaatcaac
     2281 cagataatga gggcaaaagt acccatttta gacatcaatc ctgatagtaa catggtgcct
     2341 tgatttggtg cattaacgta cgtatctgcc tgccaggaat gaaatggaaa gattggaatt
     2401 ttgatagcaa aggcaaggta aaaagctaag aacacaaaca attgttcatt tgcagacaat
     2461 ctttgtgcgg catcgtaaaa tgccaaaata ttaaatgatt gtgttactcc ttccgttcct
     2521 ccacttgtac gcaaccaaat gtaaatgatt gccagcaaca taaataaact tccgaatagt
     2581 gtgtacacaa aaaatttgaa tgtgatttgt tttctgtccg gcccacccca aattaaagac
     2641 aataagaata cgggcaataa tgccaattcc cagaaaaaat aaaacaacaa tgcatctttg
     2701 gctaagaaga cacctaccaa tgcaaattgc atcaataata tcaacgataa aaaagcttta
     2761 gttcgtttaa ttgattctgt taatgtcgac tgaataataa acggcaccat taatgtggta
     2821 agcaaaacca ttactgcact aattccatcc atagcgaact gaaatctggc attgaggctg
     2881 aatatccaag gataatctac tttaaaacaa gcacaatccg gatgcaggga gaacatataa
     2941 aaagcataaa agcccacaca catttcaacc aatgcgccaa gcaatgctat tctttttgca
     3001 ttcccgtcat tcatcaacca aatcagagga acaaaaagaa cgggaataaa taataataac
     3061 aatatcaaca tacctattta cttataaact caaaagtggt gaaaaacatg ctctcatttt
     3121 ttaattaaat taaaaccgat aataataaaa atacagctac acccaatacc atcgctatca
     3181 cgtaaaaacc caagttgcca ttttgcgtat agcgcatcat gctgctccag cttagtaccg
     3241 cactgcctat tccgttaacg acagtgtcaa ttccgctttt ctctatccat tcgtttagta
     3301 atgatgcaaa tttattcatc ggtttcacaa ttatagcttc atatatttca tccacataaa
     3361 atttgtgata cacgatttct ctccataatg atgccggttc gccgtcagct acaggtactt
     3421 ttttgtttgt aacaaaaatg tttctggcta ataaaatcac ggctaaaatg gttactatcg
     3481 taatgccaat caacatcagc tctctgttga ggttaacatc attttcagtt actgttaccg
     3541 aagagcttaa gaaaccgtct atccagtgat gaatacccaa tgcatggccg ataccatgcg
     3601 gaaatcctaa aaaaccgcca atagttgata agatagccaa tactatcaga ggaaatgtca
     3661 tagttgcagg tgattcgtgg atatggtgtt cttgttctgc tgtacctctg aatgagccat
     3721 aaaaagtaag gaataacaat ctgaacatgt aaaatgcagt cattaatgag gcaaataatg
     3781 ccaatacaaa aagtatttta ctgtgttcaa aaatttctgc cagcatttgg tctttcgaaa
     3841 agaagcccgc aaacggaggt attcctgaaa tagcaacagt accgattaag aaagtgataa
     3901 aagtaatgcg cattttatct tttaaacctc ccatatttcg gatatcctga tcgccactca
     3961 tcccatggat gacgctacct gctccaagga acaacaacgc tttgaaaaaa gcatgggtaa
     4021 ccacatgaaa aactccggca gaaaatgcgt cacatcctaa agcagtaaag attaagccta
     4081 actgagatac ggtagaataa gccagtactt ttttaatatc attttgacgc atagcaatcg
     4141 tagcagacaa gagtgctgtg gcaataccga tgatggaaat aaatgtcatg gtaaaatgag
     4201 acaaagtaaa cagcacatta caacgggcta ccatataaat accagccgta accatagtag
     4261 ctgcatgtat caatgcagaa accggcgtag gaccagccat ggcatcaggc aaccaggtat
     4321 ataatggaat ttgagctgat tttcccattg caccgataaa cagcagcaag gttatccatg
     4381 ttattaattg accgtttact ttcacatctc ctgctaagtt gaaaactgta tgatagtcga
     4441 ttgtgccaaa tacaccaaaa attaagatga tacccaaaat aaatcctaag tcaccgatac
     4501 ggttcatgat aaatgctttc tttgctgctc ctgcataatc aatattgctg aaccaaaatc
     4561 caatcaacag ataagaacac aagcctacgc cttcccaacc gacaaacatc aataagaaat
     4621 tattgcctaa tacaagcaat aacatgaaaa agacgaataa attcaggtaa gaaaaatagc
     4681 ggttataatc gggttcatca tgcatatagc ctactgaata aacatgaatg agaaaaccta
     4741 ctcccgtaat aaccagtgtg aataatatgg ataaagaatc ggcagttaaa gcaatttctg
     4801 atttgaacgt accgacatta atccatggta aaatatgcgc agttagggat tcgcctgtgt
     4861 gcagtatttt gaaaaaaaca ataactgcca caataaaaga aagcaagata cttccgggag
     4921 ctaacaggga agtgacggat ttgggcaatt tatttcccaa tacagctata atcaaaaagc
     4981 tgattaaagg aaataaaggg attagcatta gcaatgacat tcgtttttac tttttaattt
     5041 ccagaagttt gaagaactcg tctaatttcg gcatgatgat aatttccgtt ctgcggttta
     5101 tagctttatt tgccggtgta tcgttggcta ctttaggata aaattcggag cgaccgcctg
     5161 caataatacg agcaggatta actgcatatt gcaattgtaa tgcccgggca attgaagttg
     5221 ctcttttcac gcttaaatcc cagttatcca gcatacaaga attttttata ggcacattat
     5281 ctgtattgcc ttcaattaat acatcaaatg tgctgtaatc gtttatcact ttcgcgattt
     5341 ttcctaaaat ttcgcctgct cgtggtaaaa tctcatacga accggatttg tataacatac
     5401 cgtctgataa agaaatcaga acgacacctt tatctacgtt aacatttaca tccgtatcat
     5461 ttacatcttg cagg
//
LOCUS       DAOQII010000167         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8631_length_5462_cov_4.252820, whole genome shotgun sequence.
ACCESSION   DAOQII010000167 DAOQII010000000
VERSION     DAOQII010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8631_length_5462_cov_4.252820"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..969
                     /locus_tag="PLF48_08400"
     CDS             208..969
                     /locus_tag="PLF48_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF49384.1"
                     /translation="MGQIKAILVDDEKNNRIALSKLIEKFCPNVTVSGECESVDEAII
                     QIANLNPDVVFLDVEMPSKNGFDLLAHYQYHCPFDVIFTTAYSQYAVKAFRYSALDYL
                     LKPVDPEELIQAVHKLAMKLDESIKHKQFELLENKINHSDIAKKQIAVSTLEGIYFAS
                     LDEIIHVDADSSYTKIFLSNGDMIMSSKPLKYFEEMLEDFDFVRVHQSHIINLKHIRR
                     YVRGDGGQVIMTNGTEIEVSRRKKEDLLSKISETL"
     gene            complement(1207..3096)
                     /gene="rpsA"
                     /locus_tag="PLF48_08405"
     CDS             complement(1207..3096)
                     /gene="rpsA"
                     /locus_tag="PLF48_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNF49385.1"
                     /translation="MTEELDQTHLANEDADVVNHEKDVVEQEVIDIELPVAKKKSVEF
                     DWNISKKGANQYSKEKTAELQNMYDSTFRQVEDSEIVSATVTGLTDSDVILNIGFKSD
                     GMIPRSEFKDLPDLKIGDEVDVYVESKENKKGQLILSRKKAKLTKAWDLICEAHEKDI
                     IVKGKVISKTKGGLIVELYGMETFLPGSQIDVKPITDYDQFVGKTMEFKVVKINHVIK
                     NAVVSHKALIESDLESQRQEIIGKLEKGQVLEGTIKNITDFGAFIDLGGVDGLLYITD
                     ISWGRINHPNEVLKMDQKLQVVVIDYDDNKKRISLGLKQLQPHPWDSLPEGIEVGSKV
                     KGKIVNIEDYGSFLEIVPGVEGLIHVSEVTWSNQPINSRDFFKLGDEFEALVVTLDKD
                     ERKMSLSLKQMTEDPWTSIASKYPVGSKHKGLVKNITPYGVFVELDDVVGGMVHISDL
                     SWTKRYNHPSEFTKVGNDLDVIVLEIDSDNRKIVLGHKQLEEDPWDTFENLFPLGSTH
                     EATVLKREDKGAVVQLPYGIEAFAPTRHIKKADGKLAEVEEVLPFKVLEFDRDDKRII
                     VSHTRVIEDAKLDDKKKVEGEKKKEAKKAKDSVKELNSKVEKSTLGDLDALSDLKAKM
                     EGKKD"
     gene            complement(3306..4250)
                     /locus_tag="PLF48_08410"
     CDS             complement(3306..4250)
                     /locus_tag="PLF48_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF49386.1"
                     /translation="MKNQFCFLLGISLLIACHTQSKAQTTGCQGTEFRQFDFWLGNWD
                     VYNSAGKKVGENKVVLMQDSCVIQENWTSGNQTGTSYSYYNTSNKTWSQVYIDNTGNI
                     LELKGKYKNGKMILRSKPAINPKSKKSTQNVITWFQDDSGFVHQKWDIVNSKDSIIAV
                     AFDGIYKKKKNNSESSAIATGIGGVFFKSKDPKTLKKWYQDNLGFKIDQYGVMFEWRE
                     GKDADRYGMTQWSLFNNKTNYFEPSAKDFMINYRVQQLDSLVEHLKQKGVQIIDTIET
                     YDGIGKFIHIMDPEGNKIELWEPEKADFDKVMEEKKKK"
     gene            complement(4271..5329)
                     /locus_tag="PLF48_08415"
     CDS             complement(4271..5329)
                     /locus_tag="PLF48_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNF49387.1"
                     /translation="MNTFSSEAVSDDMRTKIIRKEIQHASKEARAKYAFLKHQNTIGF
                     AIFIISCLMIVVGAFFYLQHKTNWLLLAFWIAFWTSILHELEHDLIHFMYFKKNKLMQ
                     DVMMLGVWIFRPMTINPWYRRELHFHHHRTSGTKTDVEERGVTNGEKWGLKRLLTTAD
                     ILFGGIVRAHTLKRDIIDAVKSGELEKKQALEFKKINLYGMLPFTLLLYFIWYFFLIH
                     YGIHYFTKSVGIPYQSPTWINAQFVWINPLVAILIFPNLLRMFCLHFITSNMHYYGDV
                     EPGNVMQQTQILNKWYFLPFQLFCFNFGSTHAIHHFVVSETFYIRQLTASRAHKIMKA
                     QGVRFNDLGTFRRANRFH"
BASE COUNT         1611 a         1035 c          938 g         1878 t
ORIGIN      
        1 tatgcacaca acaaatatac tgtttatctc attttttcgc gcatttttgt ataaccgaaa
       61 gtgtgaggtg gattagttgt gtcatctaat tattgtgcag tgaggatatt ctaaagtgtt
      121 atcgtgctgg aacatagctt atttttttta tacaagaaaa atccaggcta tttaaaattg
      181 cttatcttca tttattgaaa tttttatatg ggacagataa aagccatttt ggtagatgac
      241 gaaaaaaata accgcatagc gttaagcaaa ctcatcgaaa aattttgtcc taatgtaact
      301 gtttccggag agtgtgaaag tgtagacgaa gcaattattc agattgcaaa cttaaatccc
      361 gatgtggttt ttttagatgt agagatgcct tcaaaaaacg gctttgattt attggcacat
      421 tatcaatacc attgtccgtt tgatgtcatt tttactacgg catacagcca atatgcggtg
      481 aaggcatttc gctacagtgc actggattat ttgttgaagc cggttgaccc tgaagaactg
      541 atacaggcgg tacacaaact tgccatgaag cttgatgaaa gtataaaaca taagcaattt
      601 gaactgttag aaaacaaaat caatcattcc gatatagcaa agaaacaaat tgctgtttct
      661 accttagaag gtatttattt tgcctcttta gatgaaatta ttcatgtgga tgcagattcg
      721 tcttatacca aaatattttt gtcgaatggc gatatgatta tgtcttccaa gccgctgaaa
      781 tattttgaag aaatgctgga agattttgat tttgtacggg tacatcagtc gcatattatt
      841 aacctgaaac atatccgcag atatgtgcgc ggcgatgggg gacaagtcat catgaccaac
      901 ggtacagaaa tagaagtatc gcgaagaaaa aaagaagact tgctgagtaa gatatcagaa
      961 acgctttaat cttgtttata aaatcaaatt attgcattat tgtcatgctg aggaaatcgt
     1021 ataaataaga atttctcttt tagaaaacca tatatgggaa ctgcattttc tgaaaaagca
     1081 gtattgtcta tttgtgcgtg tgtggttaag gaatccttcc cttattcagt tgattttgat
     1141 gttgagtaag tccataaaaa aatcccgcca ttacagcggg attttctatt aaagattgtt
     1201 ctgagattaa tcttttttgc cttccatttt agctttcaaa tcagataaag catccaaatc
     1261 gccaagtgta gatttctcta ctttgctgtt caattctttt acagaatctt tagctttttt
     1321 agcttctttt ttcttctcac cttctacttt tttcttatca tccagcttag catcttcaat
     1381 tactcttgta tgagacacga taatgcgttt gtcatcgcgg tcaaactcca gtactttgaa
     1441 tggtaatact tcttccactt cagctaattt tccgtctgct tttttgatat ggcgggttgg
     1501 tgcaaatgct tcgataccat aaggcaactg tacaactgct cctttgtctt cgcgtttcaa
     1561 aacggtagct tcatgtgtag aacctaacgg gaacaagttt tcaaatgtat cccaaggatc
     1621 ttcttctaat tgtttatgtc ctaaaacaat tttgcggttg tcgctgtcaa tttccaacac
     1681 gattacatcc aaatcattac ctacttttgt aaactctgat gggtgattgt agcgttttgt
     1741 ccatgacaaa tcagaaatat gcaccatgcc gcctactaca tcatccagct caacaaatac
     1801 accataaggc gtgatgtttt tcactaagcc tttgtgcttg ctgccaacag gatatttaga
     1861 tgcaatactt gtccatggat cttcagtcat ttgttttaag ctcaatgaca ttttgcgctc
     1921 gtctttgtct agggtaacca ccaatgcttc gaattcgtcg cctaatttga agaaatcacg
     1981 agagttgatt ggttggttgc tccaggttac ttcagatacg tgtatcaatc cttctacacc
     2041 cggtactatt tccaagaaag aaccgtagtc ttcaatgttc acgattttac cttttacttt
     2101 agaacctact tcgatgcctt caggcaatga atcccaagga tgcggttgta attgtttcaa
     2161 gcctaaagaa atgcgttttt tgttgtcatc ataatcaata accaccactt gtagtttttg
     2221 atccattttc aatacctcgt tcggatggtt gatacgtccc caagaaatat cggtgatgta
     2281 caataatcca tctacgccac ccaagtcgat aaatgcacca aagtcggtga tgtttttgat
     2341 agtaccttcc aacacctgac ctttctccag tttgccaatg atttcctgac gttgagattc
     2401 caaatcgctt tcgataagcg ccttgtgtga aaccaccgcg tttttaataa catggttgat
     2461 tttcactact ttgaactcca ttgtttttcc tacaaattgg tcgtagtcgg taattggttt
     2521 tacatcaatt tgagaacccg gcaagaaagt ttccatgccg tacaactcca cgattaatcc
     2581 tccttttgtt ttgctgataa ccttaccttt aacgataatg tctttttcat gtgcttcgca
     2641 aatcaaatcc caggctttgg ttaatttcgc ttttttgcga gaaaggataa gttgaccttt
     2701 tttgttttct ttgctttcca cgtacacatc cacttcatca ccaattttaa ggtcaggaag
     2761 gtctttaaac tcagaacgtg gaatcatacc gtcagatttg aagccgatgt ttaaaataac
     2821 atctgagtct gtcaaaccgg taactgtagc acttacaatt tcactgtctt ctacttgtcg
     2881 gaatgtagaa tcatacatgt tttgcaattc agcagttttc tctttagagt actgatttgc
     2941 tccttttttg ctgatgttcc aatcaaattc tacggatttc ttttttgcaa ccggaagttc
     3001 gatatcgata acttcttgtt ctaccacatc tttttcgtgg tttactacat cggcatcttc
     3061 atttgccaaa tgtgtttgat ctaattcttc tgtcaaaatg actaatttta aaggttaaaa
     3121 aataatttat ctctccaaaa agtgcgcaat gcaaaatttt ggactgcaaa agtactaaat
     3181 ttatttgata attaactaat tagaccaaca gattaatggg atttgatgtt atgctgttgt
     3241 gaaaaaataa gtaggtttaa atggcttgga gggtatagaa aaacaatgac tgagagagat
     3301 ataggttatt ttttcttttt ttcctccatt accttgtcaa aatcagcttt ttccggctcc
     3361 caaagttcaa ttttgttgcc ttccggatcc ataatatgaa taaactttcc tatgccatcg
     3421 taggtttcaa ttgtatctat tatttgtaca cctttttgtt ttaaatgctc tactaaacta
     3481 tcaagttgct gtaccctgta gttaatcata aagtctttag ctgacggctc aaaataattg
     3541 gttttattat taaacaaact ccattgtgtc attccatatc ggtcagcgtc tttaccttca
     3601 cgccattcaa acattacacc gtattggtct atcttaaatc ctaaattatc ttgataccat
     3661 ttcttcaatg ttttaggatc tttactcttg aaaaacacgc caccaatgcc tgtagctatt
     3721 gctgaacttt ctgaattgtt tttctttttc ttgtaaatac catcaaatgc cacggcaata
     3781 atactgtctt tactattgac aatatcccat ttttgatgta caaaaccgga gtcgtcttga
     3841 aaccaagtaa tgacattttg agttgatttt ttgcttttgg gatttatagc aggcttgctc
     3901 cttaaaatca tttttccatt tttatatttc cctttcaact ccagtatatt tcctgtatta
     3961 tcaatataaa cttgactcca cgtcttgtta cttgtattgt aataggaata acttgttccg
     4021 gtttgatttc ctgatgtcca gttttcttgt atcacacaac tgtcttgcat cagcactact
     4081 ttattctcac ctactttttt tccggcagaa ttgtatacat cccagttgcc cagccaaaaa
     4141 tcaaactgac gaaattctgt gccttgacaa cctgtagttt gtgctttact ttgtgtatga
     4201 caggcaatga gtaaagaaat ccccaatagg aagcagaatt ggtttttcat cttgtaaaaa
     4261 taattgaatt ttagtgaaaa cggttcgcac gtctgaaagt accaagatca ttaaagcgaa
     4321 cgccttgggc tttcatgatt ttatgtgctc gtgaagcagt taattgccga atgtaaaaag
     4381 tttcactcac cacgaaatga tgaatggcat gcgtactgcc gaaattaaaa cagaataact
     4441 gaaatggcaa aaaataccat ttgttcaaaa tttgtgtttg ctgcatcacg tttcccggct
     4501 ctacatcgcc ataatagtgc atgttggatg tgataaaatg aagacaaaac atgcgtaata
     4561 aattcggaaa tatgaggatg gcaaccaatg gatttatcca aacaaattgg gcatttatcc
     4621 atgtgggact ttgataagga atgcctactg attttgtaaa ataatggatg ccatagtgta
     4681 ttaaaaagaa ataccagata aagtataaaa gcaacgtgaa aggcaacatg ccatagagat
     4741 tgattttttt aaattccagc gcttgtttct tttctagttc cccggatttc acggcatcaa
     4801 taatatctcg tttcaaggta tgagcccgca cgataccgcc gaataaaata tctgctgttg
     4861 tcagtagtcg tttcaatccc cacttttcac cattggttac accgcgttct tccacgtcag
     4921 ttttagtacc cgaagttctg tgatgatgaa aatgcaactc gcgtctatac cacgggttga
     4981 tagtcatagg tctgaaaatc caaacaccaa gcatcattac atcctgcatt aatttatttt
     5041 tcttgaaata catgaaatga attaaatcgt gttccaattc atgtaatatg gatgtccaaa
     5101 aggctatcca aaatgcaagc aacagccagt ttgttttatg ttgcaaataa aaaaatgccc
     5161 cgactacaat catcagacaa gaaataataa atatggcaaa gccaattgta ttttgatgtt
     5221 ttaagaatgc atatttcgca cgtgcttctt tgcttgcatg ttgaatttct ttgcgaatga
     5281 ttttggtgcg catatcatca ctgacagctt cagatgagaa cgtattcata cattaaaatt
     5341 acgaaatagc acaacaaaaa tcaatttact ttggtaaaga gatttaaggg atagattttg
     5401 taaaattgag gatactctgt taagtaataa tcagcagtta aaaaatctaa tcggtgggga
     5461 tt
//
LOCUS       DAOQII010000168         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8639_length_5459_cov_5.122132, whole genome shotgun sequence.
ACCESSION   DAOQII010000168 DAOQII010000000
VERSION     DAOQII010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8639_length_5459_cov_5.122132"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1577)
                     /locus_tag="PLF48_08420"
     CDS             complement(54..1577)
                     /locus_tag="PLF48_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNF49388.1"
                     /translation="MNKELVIHANSKGVEIALLENKKLVEYHLESYEKEGFGVGDIYL
                     GRVKKLNPGLNAAFVDIGHDKDAFIHYSDLSPYLRTLRKFSSAAFQGEQFHLLNQLEY
                     ELPIDKNGQIQDVLEKNDILIFQISKEAISTKGPRLTCELSIAGRYIVLTPFGNGIGI
                     SKRIEVKEERERLIQLIQKFKPANFGIVVRTNAEGLSEDELKRDIEVLLDKWKIMTTN
                     CHLKNPPHLLLKEMNKSFSLVRDLVNDSFENIVTDSESIYADLKAFIKEHAPEQKSIL
                     NHYKDTTPLFETYEVSRQVKTAFGKIVTLKSGAYIILEHTEAMHVIDVNSGPKIRKDI
                     PQDEQAFNINCDAAREIARQMRLRDIGGIIVIDFIDMKSSELRHKLFEVMHEAMKPDK
                     AKHVLLPLSKFGLMEITRQRMKQQISIDTTEPLLTNQKFKIDQPLQIIDAIKTDLRNL
                     KEKKEKRFVLFVHPFIYAYLNKGKVPFRMRSYVKYGKWVKLIQDSALTLSEYKIEAY"
     gene            complement(1730..2569)
                     /locus_tag="PLF48_08425"
     CDS             complement(1730..2569)
                     /locus_tag="PLF48_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49389.1"
                     /translation="MKKQYLVATGSILLIGLLFFFGKTKKPASSMGQTSNIASGSISF
                     EEYEMQKADKLVSTDKKNWEDLHSIYKETKESDTANYLKICSDLSLFWKSQQNGSLYA
                     YYNYQRQTSVNTAKSWMDAGDVLLSVYKNNPDSLISNNLLTFALRSLENASAKDPQNI
                     SLKIQIAEIYVQTAEPMKGITMLRTLGDSLPDNMDVQMSLARLSIQSGQLEKAKERLQ
                     KILQHQPQNTEALYFMAITEAQMGHTDEAVRLLELCKTLVNNDEFSNEINNLIQEIKN
                     KKV"
     gene            complement(2583..2888)
                     /locus_tag="PLF48_08430"
     CDS             complement(2583..2888)
                     /locus_tag="PLF48_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNF49390.1"
                     /translation="MRKADIITTISEKTGVPKVDVLVTMEAFFKEVKDTLAEGENLYV
                     RGFGSFIVKTRKAKIGRNIKKNIAIEIPEHQIPAFKPSKVFVEMIKEQSAKRLSDKA"
     gene            3170..4240
                     /gene="serC"
                     /locus_tag="PLF48_08435"
     CDS             3170..4240
                     /gene="serC"
                     /locus_tag="PLF48_08435"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNF49391.1"
                     /translation="MKKHNFGAGPSIMPQEVFEQAAQACVEYENIGLSLLEISHRSKE
                     FDAIMEEMVSLIRELLKVPNDYEILFIQGGANMQFAMVPYNLLPSDGKACYLETGVWA
                     AKAEAEARLYGQTEVVASSKASGFNFIPKEYTIDKQAAYFHITSNNTIYGTELFQDID
                     SPVPLVADMSSDIFSRPVDVSKYALIYAGAQKNLGIAGITLVIVRKDMLGKNVRSMSS
                     MWNYDVYAKNNSLYNTPPVFAIYTCLLTLRWLKKLGGLTEMEVRNKEKADLLYTEIDR
                     NPLFRGHSVVEDRSRMNITFVAENPEDETAFLDFVKPYQIHGIKGYRTVGGFRASCYN
                     ALPIDSVRVLVNAMQEFELSKK"
     gene            4317..4748
                     /locus_tag="PLF48_08440"
     CDS             4317..4748
                     /locus_tag="PLF48_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49392.1"
                     /translation="MLLLEKTYNEIYKKYFDSLPWVKIDSSISIYHFIPKTNTIDTTN
                     SFDYSYSSWIDTGIVILNSFHNSDKFDTEEYAEPLTVTETSIDENTNNVTPEFRLDSY
                     NFINYLKYIYKNDEVLIRVKFVLNKNKKTTGYMYFYVEAEI"
     gene            complement(4801..5283)
                     /locus_tag="PLF48_08445"
     CDS             complement(4801..5283)
                     /locus_tag="PLF48_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNF49393.1"
                     /translation="MNEFNGEIKKMMIILSKATLENVYAAFVLANGARMEGIEAELFF
                     TFFGLEAIHKEKLEGLHIATVGNPAMHMPTMLGGLPGMESLATYMMKKEMDKLDMPGV
                     KEFLEILSASGCKLWACKLAMDMFHYKKEDLIDELDGVLTVGDFYGRAKGLGTHMLFI
                     "
BASE COUNT         1602 a          998 c          965 g         1894 t
ORIGIN      
        1 tctacataaa aaatcaacta ttttttaatt gaaatgctta tcccgaactc aggttaataa
       61 gcctcaattt tgtattcgct tagtgttaaa gcagagtctt ggatgagttt gacccacttg
      121 ccatatttta catatgaacg catacgaaac ggaactttac ctttattgag gtaggcataa
      181 ataaaaggat gaacaaaaag aacaaaacgt ttttcctttt tttcttttaa gtttcgcaag
      241 tctgttttaa ttgcatcaat aatctgcaag ggttggtcta tcttgaattt ctgattggta
      301 agcaatggtt ctgtggtatc aatactgatt tgctgtttca tgcgctgacg ggtaatttcc
      361 atcaacccaa atttactgag tggcaataaa acatgcttgg ctttatcagg cttcatggct
      421 tcgtgcatca cttcaaaaag tttgtgacgc aattcactcg atttcatatc tataaagtca
      481 atcacaatga ttccaccaat atcacgcaaa cgcatctgac gagcaatttc acgagccgca
      541 tcacaattga tattaaatgc ttgttcatcc tgtggaatat cttttctgat ttttggtccg
      601 ctgtttacat ctataacatg catggcttcg gtatgctcca aaataatgta ggcacctgat
      661 ttcagggtaa ctattttccc gaatgccgtt tttacttgcc ggcttacttc atatgtttca
      721 aatagcggtg tcgtatcttt ataatggttt agtatggatt tttgctccgg agcatgttcc
      781 ttaataaagg ctttcaagtc ggcataaatg ctttcgctat ccgttacgat attttcaaaa
      841 ctatcattta ccaaatctct gactaaagaa aaggatttgt tcatttcttt taataacaaa
      901 tgaggtggat tctttaaatg acaatttgta gtcattatct tccatttgtc caacaatact
      961 tcgatatccc gtttcaactc atcttcgctc aatccttccg catttgtgcg cactacgatg
     1021 ccgaaatttg caggcttaaa tttttgaatt agctgaatca gtcgttctcg ttcttcttta
     1081 acttctattc ttttcgaaat gccgattcca tttccaaatg gagtaagcac gatgtatctg
     1141 ccggcaatag aaagttcgca agtcagacgt ggcccttttg tagatatagc ttctttggaa
     1201 atttgaaaaa taagaatgtc gtttttttcc aaaacgtctt gaatctgccc gtttttatcg
     1261 atgggcagtt catattcgag ttgattcaaa agatgaaact gttcgccttg aaaggctgcc
     1321 gaactgaatt tgcgcaaggt tctcagataa ggactcaggt cgctgtaatg aataaacgca
     1381 tctttatcgt gaccaatgtc aacaaatgcg gcatttaatc ccggatttaa tttctttact
     1441 ctgccgagat aaatatcacc tacaccgaac ccttcctttt cataggattc taaatgatat
     1501 tcgactaatt ttttgttttc aagaagcgca atttcaacac cttttgaatt agcatgtatt
     1561 actaattctt tattcatcgt agttgaaagt cattagttgc ctattgctat attgcaaata
     1621 agcaacggtt aatgttttac ttctttttgt gacgattttt tctcagtcgt ttttttcttt
     1681 tgtgcgtcgc gattttgtga cgttttcttt tttttccgca aggcataatt tatacttttt
     1741 tattttttat ttcctgaatg aggttattaa tctcattgct gaattcatca ttattcacca
     1801 acgttttaca gagttccagc aagcgtactg cttcatctgt atgccccatt tgagcttcgg
     1861 tgatagccat aaaatacagt gcttctgtat tttgaggctg gtgttgcaga attttctgca
     1921 ggcgttcttt agctttttct agttgcccgg actgaatact caatctggct aatgacattt
     1981 gcacatccat attgtcaggc aagctgtcac ccaatgtacg caacatcgtg atgcctttca
     2041 tcggttcagc cgtctgtaca tagatttcag ctatttgaat ctttaaactt atgttttgag
     2101 gatcctttgc tgaagcgttt tctaaagaac ggagcgcaaa ggtaagcaaa ttatttgaaa
     2161 ttaagctgtc cggattgttt ttataaacag aaagcagtac atctcccgca tccatccatg
     2221 attttgcagt gttaactgat gtttgacgct gataattata ataggcatac aagctgccat
     2281 tttgctgact tttccagaaa agtgacaagt ccgaacaaat tttgaggtag ttggctgtgt
     2341 cgctttcttt ggtttcttta taaatagagt ggaggtcttc ccagtttttt ttatccgtag
     2401 aaaccagctt atcagctttt tgcatttcat attcttcaaa tgaaatagaa ccggaagcaa
     2461 tatttgacgt ttgccccatt gaggaggcag gtttttttgt ttttccaaaa aagaacagaa
     2521 gacctatcag taaaatactt cctgtcgcta ccagatactg tttcttcaca acaagatttt
     2581 gattatgcct tatcgcttag acgcttagca ctttgttctt taatcatctc tacgaatact
     2641 ttagagggtt taaaagccgg aatttggtgc tccggaattt cgatagcaat attcttttta
     2701 atatttctac ctattttggc tttgcgggtt ttaacgataa agctgccgaa gccacgaaca
     2761 tataaatttt caccttcagc caacgtatct ttcacttctt taaaaaatgc ttccatagta
     2821 acgagtacat ccacttttgg cacacctgtt ttctcagaaa ttgttgttat aatatcagct
     2881 tttctcatta gttacgaata ttttatagtg caaaaatggt aaaaaaaaac tatatttttt
     2941 gcatttttat cacgagttat aatttagtta atatacgatt ctgcataaat cgggaataag
     3001 atgtgcttta tttccttaaa ctattaatct tcttaatgat gcctgcatgt aggtatttaa
     3061 aaatcgtccg gctcaaattc catattcggt acatttaaac gtattcttca cactacttta
     3121 atgtaatgct ataatcgaat gcgttatttt cgttatagaa aattaaggta tgaaaaaaca
     3181 caatttcggt gcgggacctt ctataatgcc acaagaggtt tttgagcaag ctgcacaagc
     3241 atgtgtagaa tatgaaaaca taggattatc cttactggag atatcgcatc gaagcaagga
     3301 atttgatgcc attatggaag aaatggtttc gctgattcgg gaattgttga aagtgccaaa
     3361 tgattatgaa attttgttta ttcaaggcgg tgccaatatg caatttgcta tggtgcctta
     3421 caatctcttg ccgtctgacg gcaaagcctg ctatttagaa acgggagttt gggctgctaa
     3481 ggcggaggct gaagcacgcc tttacggaca aacggaagtg gtggcttctt ctaaagcaag
     3541 tggatttaat tttattccga aagaatacac cattgataaa caggccgctt atttccacat
     3601 tacctcaaac aatactattt atggtacgga gctgtttcag gatatagact cgccggtacc
     3661 attggtagcc gatatgagtt ccgatatatt cagccgcccg gtggatgttt caaaatatgc
     3721 tttgatatat gccggcgcac agaaaaattt aggtatagct gggattacgt tagtgatagt
     3781 gcgcaaagat atgctgggaa aaaatgttcg gtctatgtct tctatgtgga attatgatgt
     3841 ttacgccaaa aacaattcgc tttataacac acctccggta tttgctattt atacgtgttt
     3901 attaacctta cgatggttga aaaagctggg tggactaact gaaatggaag tgcgcaataa
     3961 agagaaagcc gatttattgt atacagaaat agaccgcaat cctttgttta gaggacactc
     4021 tgtagttgaa gaccgcagtc gaatgaacat tacttttgta gcagaaaatc cggaagatga
     4081 aacggcattt ttagactttg taaaacctta ccagatacat ggcataaaag gctaccgcac
     4141 ggtaggcggg tttagggcat cttgttataa tgcattgccg atagacagtg tgcgagtact
     4201 ggtaaatgcc atgcaggagt ttgaactgag taaaaaataa tttacattat tttacccgta
     4261 aaaattattc ttaacaaaat tgcgtttatt acttgaattc agcgtataaa aaaaacatgt
     4321 tgttacttga aaaaacgtat aatgaaattt ataaaaaata tttcgactcg cttccatggg
     4381 tgaaaattga tagtagtatc tctatttacc attttattcc gaaaacgaat actattgata
     4441 ccacaaactc atttgattat tcatattctt cttggataga tactggaata gtaatcttaa
     4501 attctttcca taattcagac aaatttgata cagaagaata tgctgaaccg ctcaccgtaa
     4561 ccgaaacaag tatagatgaa aatacaaata atgttactcc tgaattcaga ttagattcct
     4621 acaattttat taattacctg aaatatattt ataaaaatga tgaagtactt attcgagtaa
     4681 agtttgtact aaataagaat aagaaaacga caggttatat gtatttttat gtagaagctg
     4741 aaatatagct attgcataaa atttgaaaat agtttgcaaa agaatggctt ttaatttctt
     4801 ttagataaac aacatgtgtg tacccaaacc tttagctctt ccgtaaaaat cgccaacggt
     4861 taatacgcca tctaattcat ctatcaggtc ttcttttttg tagtggaaca tatccatcgc
     4921 taatttacaa gcccatagct tacaacctga tgctgataaa atttccagaa attcttttac
     4981 gcccggcata tccaatttat ccatctcttt tttcatcata taggttgcca gtgattccat
     5041 acccggcaaa ccgcccagca tagtaggcat gtgcatggca gggttgccca ctgtagcaat
     5101 atgcaaacct tctaattttt ctttgtgtat ggcttccagc ccgaaaaagg taaaaaacag
     5161 ttcggcttct atgccttcca tgcgagcacc gttggctagc acaaatgcgg catatacatt
     5221 ttctaaagtg gctttagaca agatgatcat catcttttta atttctccat taaattcgtt
     5281 catgatatta gttattggtg attagtaatt agttatttgt tatttgttat ttgttatttg
     5341 ttatttgtta tttgtgttga gttagttatt gtagaataaa tctcactact tgctacttaa
     5401 tacttaatac tcactactta ctacttacta ctcattacaa tcttggttct tgattcttg
//
LOCUS       DAOQII010000169         5456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8651_length_5456_cov_4.427699, whole genome shotgun sequence.
ACCESSION   DAOQII010000169 DAOQII010000000
VERSION     DAOQII010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8651_length_5456_cov_4.427699"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /gene="rodA"
                     /locus_tag="PLF48_08450"
     CDS             <1..1058
                     /gene="rodA"
                     /locus_tag="PLF48_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNF49394.1"
                     /translation="FIQFISYPMYVFVCFLLLLVLFVGQKTKGDQNWINLGFFKLQPT
                     EFAKFATAMALAKYFDTPNMRFVTPREHLIAYAIVGLPLLLVAAQGDAGSCLVFLSFI
                     FVFNREGLSDVFIYLGLLAIGIAVLSLVVNKYIIIGICLAIGAILIYWNWLNKNMVKL
                     IGIITISACILSASVSLVFNNLLEKHQRDRINVILGKEISKEAKRGIAFNLNQSKIAI
                     GSGGIFGKGPLHGTQTRYNYVPETSTDFIFSAVGEEFGFVGSIILIGLFVTMMQQLLI
                     LANRQRSAYGRVYIYSVVAIIFAHFTINIAMTIGLLPVIGIPLPFMSYGGSSLISFSF
                     MIATALKLDSERRMTMR"
     gene            complement(1092..1511)
                     /locus_tag="PLF48_08455"
     CDS             complement(1092..1511)
                     /locus_tag="PLF48_08455"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNF49395.1"
                     /translation="MKNEKQISFLSGLIHRYFPETEIRVAALLADSKVLEFFSKRIYA
                     KIDSSVQFYADNAERLTYLIEIIGAWQQKEYTDIHVSKLFVGGFVLVYTASPIDIIPD
                     FIPFIGRLDDKLFLNKLMNIFDDEIEKYKEFKFRQEK"
     gene            complement(1508..1894)
                     /locus_tag="PLF48_08460"
     CDS             complement(1508..1894)
                     /locus_tag="PLF48_08460"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNF49396.1"
                     /translation="MQNFFDSLYKKAEQTITSGTAISELIDEVFLKIGEASESFYKIQ
                     DTVLAMARMLRSWFNGDYKNISAKSIIAVVAALIYFVNPFDLIPDFIPLIGQIDDILI
                     LGYLIKIVNKEIERFMTWEENQSAVK"
     gene            complement(1909..3039)
                     /gene="nspC"
                     /locus_tag="PLF48_08465"
     CDS             complement(1909..3039)
                     /gene="nspC"
                     /locus_tag="PLF48_08465"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNF49397.1"
                     /translation="MSINFQKIPSPCYVLSEELLLNNLRKLHTFQQDAGVHIICALKG
                     FSFHAVFPLVRQYLHGATASSLHEAMLVYEKMGLKAHTYCPIYLPKEFDAIQNISSHI
                     TFNSLSQLEQYRSHMRTDISYGIRVNPGYSEIKTDLYNPASPNSRLGIPLEQLGDALP
                     DGIDGLHFHAMCENNSYTLERLLDTFSKKYHKYILQAKWINMGGGHLITHREYDIAHG
                     IRVLKDFKAKYPHLQDIILEPGSAIGWKTGYLVSTIIDIVPNGKIPTAMLDVSFTCHM
                     PDCLEMPYQPKVLGETENGKFEYNLGGMSCLAGDVMGTWKFDTELKVEDRIVFDDMIH
                     YTMVKTTTFNGINLPDIGIWKTDDSFELLETYGYEDYKKRLS"
     gene            3391..4737
                     /gene="argH"
                     /locus_tag="PLF48_08470"
     CDS             3391..4737
                     /gene="argH"
                     /locus_tag="PLF48_08470"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNF49398.1"
                     /translation="MAKIWQKKASGTNELSQKVEKFTVGNDREADLKLAKYDVLGSLA
                     HCKMLNDIKLLTKMEYQAIKKALQAIYHEITEGDFSISDTCEDIHSEIEFRLTQQLGD
                     VGKKIHSGRSRNDQVLLDLKLYSRDALLQIALETITLFNTLCELSEKHKNNLMPGYTH
                     LQLAMPSSFGLWFGAYAESLVDDVQQLYSAFLLCNKNPLGSGAGYGGSFPLKRKTTTA
                     LLGFQELNYNSVYAQMNRGKMEKTVLTAIAAVASTLSKLAMDCCLFMNQHFGFISFPD
                     ELTTGSSIMPHKKNPDVWELIRARCNKIQTLPNQVQTIINNLPSGYHRDFQILKTDYL
                     AAFDEITECLDMTNFMLNHIQVSKNILNDKFYDYLFTVETVNELVLKGMSFRDAYIKV
                     GLDVEQGKFKPKRKLNHTHEGSIGNLNTMEIKAMMKTTFAQFPLFEIEEKFNSLLS"
     gene            complement(4844..5308)
                     /locus_tag="PLF48_08475"
     CDS             complement(4844..5308)
                     /locus_tag="PLF48_08475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49399.1"
                     /translation="MANKATIHFTIKHFDELSSKEVYELLLLREEVFQLEQNCLYKDI
                     DGKDIYCYHVLLYVNEELAAYTRIVPEGISYRDFVSIGRVVSSSKWRNEGMGRNVMQK
                     TMEVMDELFPNQDIKISAQAYLQKFYEDFGFVKISEPYLEDDIPHIAMVYKK"
BASE COUNT         1721 a         1006 c          952 g         1777 t
ORIGIN      
        1 tgtttattca gtttatttct tacccgatgt atgttttcgt ttgttttctg ttattgctgg
       61 tattatttgt cggacaaaaa actaaaggag accaaaactg gataaatctg ggatttttca
      121 agttgcaacc caccgagttt gccaaatttg ccacagcaat ggctttagcc aaatattttg
      181 acacgcctaa tatgcgcttt gttacaccgc gcgaacatct tattgcctat gctattgttg
      241 gtttgccttt attattagta gctgcacaag gtgacgcagg ctcgtgttta gtttttttat
      301 ccttcatctt tgtattcaac cgcgagggat tatctgatgt ctttatctat ttgggtctgt
      361 tggcaattgg catagctgta ctctctttag tcgtcaataa gtatatcatc attggcattt
      421 gcttggcaat tggggcaatt cttatttatt ggaattggtt aaacaaaaac atggtaaaat
      481 tgattggcat tattactata tcggcttgca tactctcggc atctgttagt ttggtgttta
      541 ataacttact ggaaaaacac cagcgcgacc gtatcaatgt tattttgggt aaagaaattt
      601 ctaaagaagc taagcgtggt atagcgttca atttaaatca atcgaaaatt gctattggct
      661 caggcggaat ttttggaaag ggtcctttgc atggtacaca aacacgttat aactatgtac
      721 cggaaacatc tacagatttt attttttcgg ctgttggaga agaattcgga tttgtaggtt
      781 ccatcatact aattggcttg tttgtaacca tgatgcagca acttttgata ttggccaacc
      841 gccagcgaag tgcttacggg agagtatata tttattcagt agtcgctatt atttttgcgc
      901 atttcacaat taatattgct atgaccatcg gcttattgcc cgtgatagga attcctttgc
      961 catttatgag ctacggaggc tcgtctttga tttcattttc atttatgatt gcaactgcat
     1021 taaaactcga cagtgaacgc agaatgacga tgagataaat ataaatgaat taatctcact
     1081 taactcaaca actacttttc ttgacggaat ttaaattctt tgtacttttc aatttcatca
     1141 tcaaagatat tcatcaattt attcaagaat agtttatcgt ctaagcgacc tatgaatgga
     1201 ataaaatcgg gaatgatatc tattggacta gctgtataaa caagtacaaa tccaccgaca
     1261 aataattttg agacatgaat atcggtatat tctttttgct gccaggctcc tataatttca
     1321 atcaagtagg ttaaccgctc ggcattatcc gcataaaact gaacagaaga atctattttt
     1381 gcataaatgc gttttgaaaa aaactccagt acttttgagt ctgctaaaag agccgcaact
     1441 cttatttccg tttcgggaaa ataccgatga attagtcccg acaaaaaact gatttgtttt
     1501 tcatttttca tttaactgcg gattgatttt cttcccatgt cataaatctt tcgatttctt
     1561 tattgacaat tttaattaaa tagcccaaaa tcaagatatc atctatttga ccgattaaag
     1621 gtataaaatc aggtatcaaa tcgaatggat ttacgaaata aatcaatgct gcaacaactg
     1681 caattatcga tttggctgat atgtttttat agtctccatt aaaccaggaa cggagcatcc
     1741 tagccattgc caaaacagta tcttgtattt tatagaaaga ttctgaggcc tctcctattt
     1801 ttaaaaacac ctcgtcaatc aattctgata ttgccgtacc agaggttatt gtctgttcgg
     1861 cttttttata taaagaatca aaaaaatttt gcatattcct atattcaatt aactcaatct
     1921 tttcttataa tcttcgtagc cgtatgtttc cagtaactca aagctgtcat ctgttttcca
     1981 aatgccgata tccggaagat taattccgtt aaaagtagta gtttttacca tagtataatg
     2041 tatcatgtca tcaaacacaa tcctgtcctc tacttttaac tccgtatcaa atttccatgt
     2101 tcccattaca tcgcccgcca aacaactcat tccgcctaaa ttatattcaa acttgccatt
     2161 ttctgtttca ccaagtactt taggctgata gggcatttcc agacagtccg gcatatgaca
     2221 tgtaaatgac acatcaagca tagcagtcgg tattttccca tttggcacta tatctattat
     2281 agttgatacc aaatatccgg ttttccaccc aatggcgctt cccggttcta aaataatgtc
     2341 ttgtaaatgt gggtattttg ccttgaaatc cttcaacaca cggataccat gagcaatatc
     2401 atattctctg tgagtgataa gatgtccacc acccatattt atccatttcg cttgcagaat
     2461 atatttatga tattttttag aaaaagtatc cagcaaacgc tccagtgtat acgaattatt
     2521 ttcacacatg gcatgaaaat gcaagccgtc tataccatcg ggtaaagcat cacccaattg
     2581 ttccaaaggt atgccaagtc gggagtttgg agatgccgga ttatataaat ctgtttttat
     2641 ttcagaataa cccgggttta cacgaattcc atagctgata tccgttctca tatgagagcg
     2701 atattgttct aattgagata aagaattgaa tgtgatatga ctggatatat tttgtatggc
     2761 atcaaattct ttaggcagat atatcgggca gtaagtgtgt gcttttaagc ccattttttc
     2821 atagaccagc atggcttcat gcaacgagct tgccgtggca ccatgcaagt attgtctaac
     2881 taatggaaac actgcatgaa aggaaaatcc tttcaaagcg caaataatgt gtacacctgc
     2941 atcttgctga aatgtatgta gcttgcgtaa attgtttaat agcaattctt cagacaagac
     3001 ataacaggga gaaggaattt tttgaaaatt gatggacata gttggctgta aattagaaat
     3061 aattctgctt cacaacaaaa atattttcta aaaaaaatgc gtattgacta cttttttacg
     3121 gctaactcgc aaacgtgact tcatgatacg attttactta aaaattattt tgaataaatc
     3181 cgttacagat ttctcgaaag tcagaaataa agtttgcatc aactattttt cagcatgtta
     3241 tgattcagaa atcaatttta ttagtcaact tcacggttca ttcctcactt caataattgc
     3301 ataaaaatta aaataggttt aacagaaaat tgtactaatc attttccatt tacatagtag
     3361 ctctcttttg cgaattttgt atatttagcc atggcaaaaa tatggcaaaa aaaagcttcc
     3421 ggcacgaatg aactcagtca gaaagtagaa aaatttacgg taggcaacga cagagaagcc
     3481 gacctgaaac tggcaaaata tgatgtatta ggttcactgg cacattgcaa aatgttgaat
     3541 gatatcaaat tgcttactaa aatggaatat caagccatta aaaaagcatt acaagccatt
     3601 taccacgaaa tcactgaagg agattttagt atttcggata catgcgaaga cattcactca
     3661 gaaatagaat tcagactcac tcagcaatta ggtgatgtcg gaaaaaaaat acattcgggc
     3721 agaagccgga atgatcaggt tttattagat ttgaaattgt attcgagaga tgccctgttg
     3781 cagattgcac ttgagaccat tactttattc aatacacttt gtgaactcag cgaaaagcac
     3841 aaaaataatt taatgcccgg ctatacacac ttacagttgg ctatgccatc ttcatttggg
     3901 ctatggtttg gagcttatgc tgaaagttta gtagatgatg tacaacaact atattccgcc
     3961 tttttgcttt gcaataaaaa tccgctagga tcgggtgccg gttatggcgg ttcatttccg
     4021 ctaaaaagaa aaacaactac ggccttatta ggctttcaag aactgaatta taattccgtt
     4081 tatgcacaaa tgaacagagg caaaatggaa aaaacggtac tgacagctat tgctgcagta
     4141 gcttccactt taagcaaact ggccatggat tgttgtttat ttatgaatca gcattttggt
     4201 tttatttcgt ttccggatga actgaccaca ggctcatcaa ttatgccaca taaaaagaat
     4261 ccggatgttt gggagctaat acgtgcacgg tgcaataaaa tacagacttt acccaatcaa
     4321 gtgcaaacca ttatcaacaa tctgccctca ggttatcatc gagattttca aatcttaaaa
     4381 acagactatt tagcggcatt tgatgagata acggaatgcc tagatatgac taattttatg
     4441 ctcaatcata ttcaggtaag caaaaacatt ttgaatgaca aattctacga ttatttgttt
     4501 acagtggaaa cggtgaatga actggtacta aagggaatgt cattcagaga tgcttatatt
     4561 aaagtaggtt tggatgtgga acagggtaaa ttcaaaccca aacgaaaatt gaaccataca
     4621 cacgaaggca gtatcggcaa cctgaataca atggaaataa aagcgatgat gaaaacgacc
     4681 tttgcacaat ttccactatt tgagattgag gaaaaattca atagcctttt gagttaatac
     4741 agaagaaatt attctaccta cccctgcccc ctccttttct aaggaggggg aattaaaggg
     4801 ggtggttatt attactaaca aacctgaagc aagtaatgta caattacttt ttatacacca
     4861 ttgcaatatg tggaatatcg tcttctaaat agggctcact gatttttaca aatccgaaat
     4921 cttcataaaa cttttgcaag taggcttgtg ccgatatttt tatatcttga ttcgggaata
     4981 attcgtccat tacctccatc gttttttgca tgacatttct tcccatgcct tcatttcgcc
     5041 attttgatga tgacaccaca cgacctatgg aaacaaaatc ccggtaagaa atgccctcgg
     5101 gaacaatgcg tgtatatgcc gctaattctt cattcacata caacaaaaca tgataacaat
     5161 aaatgtcttt gccgtcaata tctttgtaca agcaattttg ttcgagctga aaaacttctt
     5221 ctctaagtag taataattcg taaacttctt ttgacgacaa ctcgtcaaaa tgttttatag
     5281 taaaatgtat agttgcttta ttcgccaaaa tattcagctg taatatgttc ggtctcctga
     5341 attttaacag catgcaaaat gcatcctttt tcagtaagta tgggatgcaa ttcattccag
     5401 attgccacaa ctaaagtctc gcaggtaggt tgtgcatttt tgggcaagaa attatc
//
LOCUS       DAOQII010000170         5439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8690_length_5439_cov_4.085253, whole genome shotgun sequence.
ACCESSION   DAOQII010000170 DAOQII010000000
VERSION     DAOQII010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8690_length_5439_cov_4.085253"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2574
                     /locus_tag="PLF48_08480"
     CDS             <1..2574
                     /locus_tag="PLF48_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNF49400.1"
                     /translation="KIINPLMDAGEMPALQSLVENGVCGNIATIDPPLSPMLWTSIAT
                     GMRPHKHGILGFLEPDQSGKNLRQTHITSRKVKAIWNILNQNDKKCNVISWWPSHPAE
                     PVNGTIISNFYQTANFAEDAEHWELKKGCVYPENMHDFYASLRVHPTELTPNHLLPFV
                     PKINEINQEKDASLLKLAKNIAETASIHNAFTYLLENDKEWDFFGVYYEAPDLIAHGF
                     MKFHPPKMDHIPQHYFDIYKDVVNGIYRFMDMCLDRTLQLIDEDTTVILLSDHGFHSD
                     HLRPKSLPKEPAAIALEHSPYGIFVAKGPGIKKDERVYGASVLDITPTILQIFNLPVG
                     ADMDGKPLNTIFDHAVKSKVIPSWEFVEGNTGQHPKDLNENSYEVKDDKALKQLADLG
                     YIEAPGESSNDDVNFNNNLLENKFYLARSYNNAFQYSQAIDILEEIYIQKPVKLRFAK
                     YLLECLLNINHTERAWEIYHDLKTLKESNEAEQKADENNKTKKTSDIISENTLLQIEA
                     ALFMQEKKYSKALSILESIYKHAKMTDSKLPLADAYFKTKNYDKAISVYENEIVLNAQ
                     NSHAYYQIGLCYYFKEDYSIAIDKFLDSIGLLYFNPIAHYYLGEAFYKEKYIEEATQA
                     FEVAIRMSPRMYKARKRLKEIYRDDLKNEARLNEIFKDDIYENSPVIYIVSGLPRSGT
                     SLMMQMLEAGGLSIYTDENRKADENNPKGYYEHDAVKSIQKNNKWLGEAVGKTVKIVS
                     HLLPFIPMQYKYKIIYMERELDEVLNSQTKMLQHLGKLPENTIHISLEQNFKRNELKT
                     KEWLEDKLNVDVLYINYNELIEDSIEITAAISDFLAIPLDLEKMILKIDKSLYRNKKN
                     V"
     gene            2612..3214
                     /locus_tag="PLF48_08485"
     CDS             2612..3214
                     /locus_tag="PLF48_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49401.1"
                     /translation="MKTNTKKIAGYSAMALAAAAVLPISCKKDKAISDDPEIDVITPN
                     LSLTAPSGAIDSSTTLLGNEIDLIVGKYYSDDVVYIDFDGNSYFQKDNDVTVNGTTWD
                     FLPILNEGATIDTASAAWLDGSDNDDGYFFVKDNVPAPGASHGINGQGDKFVAFRQGS
                     GSSFKYGWMKVNVSSDGKTFVLKEMAMSNVFNKVIKVGAK"
     gene            3430..3906
                     /locus_tag="PLF48_08490"
     CDS             3430..3906
                     /locus_tag="PLF48_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49402.1"
                     /translation="MQVASTLDFTKYTPDELCHYLEHKHFEHIKHQFEAALVYFQELH
                     DEHLYIETEHLLVLLFKKLKEEVLQLIRRDTRIIFPLISNNENQPSFVSSLQAHKRII
                     DILQKIRNLTNNYIQQPEWSKTYKFCCIELLELEEQIQEVIYIKENFILQKPMLHE"
     gene            3899..5425
                     /locus_tag="PLF48_08495"
     CDS             3899..5425
                     /locus_tag="PLF48_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNF49403.1"
                     /translation="MSNAKPDLSTAKLNCFHCGDECTTKSIHLQEKYFCCNGCKTVYE
                     ILNNNGLCTYYDINEHPGTSQKIEVRKDKFAYLDDEQILQKIIQFQDEKQIHLTLYLP
                     QMHCSSCLWLLENLHTINKGIVTSKVDFEKKEVFIVLHKETITVRQVAELLTGIGYEP
                     YISFDTINNTKVSTYNKSRLYKIGVAGFCFGNIMLLSFPEYFSINGEEIDGYKHIFRT
                     INLILALPVFFYSATEFFQTAWGGIKNKYLNIDVPIALAIIITFVRSLYELYTNTGPG
                     YFDSMTGIVLFMLTGRILQDRTYQSLSFDRDFKSFFPITVNVIKDNLSAPTTLDKLKY
                     GDVFEVFSNELIPVDAILTKGNAEIDYSFVTGESIPVHKQIGEIIYAGGKQIPGKIEL
                     MVVKEVTQSYLTNLWNKSAFKKSDEYKDSYVDFIGKYFTWIVFGIALISCTYWLLHHE
                     YKLAFNVLTTILIVACPCAVALAAPLVYSIALDRLASKDLYLESVDVFTKIKKIKNRL
                     "
BASE COUNT         1896 a          906 c          961 g         1676 t
ORIGIN      
        1 aaaataatca atcctttaat ggatgccggt gaaatgcctg cactacaatc attggtagaa
       61 aatggagttt gtggcaatat tgcaactatt gaccctccac tttctcccat gttgtggacc
      121 tctattgcaa ccggtatgcg tcctcataaa cacggtattt tgggatttct agaaccggat
      181 caaagtggaa aaaacttacg gcagacacat atcacctcca gaaaagtgaa ggccatctgg
      241 aatattctaa atcaaaacga taagaaatgt aacgtcatca gctggtggcc atcacatccg
      301 gcagaacctg taaatggtac aattatttcc aatttttacc agacagccaa ttttgcggaa
      361 gatgctgaac attgggaatt gaaaaaaggc tgtgtttatc cggaaaatat gcatgatttt
      421 tatgcatcat tgcgtgttca tcctacagaa ttgacaccaa atcatttatt gccttttgtg
      481 ccaaaaataa atgaaatcaa tcaggagaaa gatgcctctt tattaaaatt agccaaaaac
      541 attgctgaaa ctgcctctat ccataatgca tttacatatt tactcgagaa tgacaaagaa
      601 tgggatttct ttggcgttta ttacgaagca ccagatttga tagcgcatgg atttatgaaa
      661 tttcatccac ccaaaatgga tcacattccg cagcattatt ttgatatcta taaagatgta
      721 gttaatggta tttatcgttt tatggatatg tgcttagaca gaacactgca attaatagat
      781 gaggatacaa cagtaatttt gttatcagat catggatttc attccgatca tctgcgccct
      841 aaatccttgc ctaaagaacc ggcagcaatt gctttagagc attcacctta tggtattttt
      901 gttgcaaaag gtcctggaat aaagaaagat gaacgtgtat atggagcttc agtgctggat
      961 attacgccaa caattttaca gattttcaac ctgcctgttg gtgctgatat ggatggcaaa
     1021 cctttgaata ctatttttga tcatgcagta aaatctaagg ttatacccag ttgggagttt
     1081 gtagaaggca atacaggaca acatcctaaa gatttgaatg aaaacagcta tgaagttaag
     1141 gatgacaaag cgctgaaaca actggcagac ttgggataca tagaagcccc cggagaatca
     1201 agcaatgatg acgtaaactt taataacaat ctgcttgaga ataaatttta tttagcccgt
     1261 tcgtataaca atgcctttca atattctcaa gctattgata ttttagaaga aatttatata
     1321 caaaaacctg taaaattacg ttttgctaaa tatcttttag aatgtctgtt aaatatcaac
     1381 catacggaaa gagcctggga aatttaccat gatttgaaaa cactgaaaga atcaaatgaa
     1441 gcagaacaga aagcagatga aaataataaa accaaaaaaa catccgatat tatttcagaa
     1501 aatacactct tacaaataga agcagctttg ttcatgcaag aaaaaaaata cagtaaggca
     1561 ttatctatac tggaaagcat ttataagcat gcaaaaatga ccgactctaa gcttccttta
     1621 gcagatgctt atttcaaaac gaagaattat gataaggcta tttccgttta cgaaaatgaa
     1681 attgtattaa atgctcaaaa tagccatgct tattaccaga ttggactgtg ttattatttt
     1741 aaggaagatt attcaatcgc catagataaa tttttagatt cgattggact tttatatttt
     1801 aatccaattg cacattatta tttaggtgag gctttttata aagaaaagta tatagaagaa
     1861 gctactcagg cttttgaagt tgcaatcagg atgtcgccac gaatgtataa agcacgcaaa
     1921 agattaaaag aaatttatag ggatgatttg aaaaatgaag caagattaaa tgaaattttc
     1981 aaagatgata tttatgaaaa ctcgccagtt atttatatcg tttcaggatt accaagaagt
     2041 ggtacgtctt taatgatgca aatgctggaa gccggaggat tatcaattta taccgatgaa
     2101 aaccgaaagg cagatgaaaa taacccaaaa ggatattatg aacacgatgc tgtaaaatct
     2161 attcaaaaaa ataataaatg gctgggtgag gctgttggca aaacagtaaa aattgtttct
     2221 catcttttgc catttattcc aatgcagtat aaatataaaa ttatttatat ggaaagagag
     2281 ttggatgaag tgttaaattc tcaaactaaa atgctgcaac atttgggaaa acttccagaa
     2341 aacacaatac atatttcact ggaacaaaat tttaaaagaa atgaactcaa aaccaaagaa
     2401 tggctcgaag ataaactaaa cgtagacgta ttgtatataa attacaatga acttatagaa
     2461 gacagcattg aaattacagc tgctatatcc gattttttag ctattccatt agatcttgaa
     2521 aaaatgatat taaaaattga caaaagcctt tatagaaata aaaaaaatgt ttaactttat
     2581 aaaactcaaa ttaactttat aaactcaaat tatgaaaaca aacacaaaaa aaatcgcagg
     2641 ctactcagcc atggcattag cagcagcagc agtgcttcca attagttgta aaaaagacaa
     2701 ggcaatttca gatgatccgg aaattgatgt tattacacct aatcttagcc ttactgcacc
     2761 ctcaggagct atagacagct ctacaacttt attaggcaac gagatagatc ttattgttgg
     2821 gaaatattat agtgatgatg tagtatatat tgattttgat ggcaattctt actttcaaaa
     2881 agataatgat gttacggtga acggtacaac ttgggatttt ttaccaatcc ttaatgaagg
     2941 tgcaactatt gatactgctt ctgctgcttg gttggatggt agtgataatg atgatggcta
     3001 ctttttcgta aaggataatg tccctgcgcc cggagccagt catggtatca atggccaagg
     3061 cgataagttt gttgctttca gacaaggttc gggaagcagt tttaagtacg gatggatgaa
     3121 agtaaatgtt tccagtgatg gaaaaacatt tgtactaaaa gaaatggcaa tgtcaaatgt
     3181 attcaataaa gttattaaag taggtgctaa ataattttca gcttaaatta caatataaag
     3241 gttgcacaag gtgtaacctt tttttttata tacccggcaa atatttcatt tcttgaaaca
     3301 gcttactgtc tcatcctgaa acaggattac attttttact caaaaaactg ataaaaatca
     3361 tgttttccat tgatgtaact caattacaac taattacttc gctacttact tttacctaaa
     3421 ataagtatag tgcaagtagc ttctactttg gattttacaa aatatactcc ggatgaactt
     3481 tgtcattatc tggagcataa gcattttgaa catatcaaac accagtttga ggcggcactc
     3541 gtttattttc aggaactgca tgacgaacat ctgtatatcg aaaccgaaca cttgttagtc
     3601 ttattgttta aaaaactaaa agaagaggta ttacagttga ttcgtagaga tacacgcatt
     3661 atttttcctt tgattagtaa taatgaaaac caacctagtt ttgtatccag cttacaagca
     3721 cacaaacgca ttattgatat ccttcaaaaa ataagaaacc ttactaataa ctatatccaa
     3781 cagccggaat ggagtaaaac ctataaattt tgctgtatag aattgttaga actcgaagaa
     3841 cagattcagg aagtcattta tatcaaagaa aacttcattc tgcaaaaacc aatgttgcat
     3901 gagtaatgcc aaaccagatt tatcgaccgc taaactcaac tgcttccatt gcggcgatga
     3961 atgcaccaca aaatccattc accttcaaga aaaatatttt tgctgtaacg gatgtaagac
     4021 tgtttacgaa attttaaata ataacgggct ttgtacgtat tatgacatca atgaacatcc
     4081 gggtacttca caaaagattg aggttcgcaa ggataagttt gcatatttag atgatgaaca
     4141 aattcttcaa aaaattattc agtttcaaga tgaaaaacaa attcatctta ccttgtattt
     4201 gccacaaatg cattgcagct cttgtttatg gcttttagaa aatctgcata cgatcaataa
     4261 gggtatcgtg acttccaaag tagattttga aaaaaaagaa gtttttatcg tattgcacaa
     4321 agaaacaatc acggttcgtc aggttgcaga gttgctcaca ggtataggtt atgagcctta
     4381 tatttcattt gataccatca acaacaccaa agtatcaact tataataaga gcagactata
     4441 taaaattggt gttgccggtt tttgttttgg taatattatg ctgttgagtt ttcccgaata
     4501 ttttagtatc aatggcgaag aaatagacgg ctataagcat attttcagaa cgataaattt
     4561 gattttagcc ctacctgttt ttttctattc agctactgag ttttttcaaa cggcatgggg
     4621 aggtattaaa aataaatacc tcaatattga tgtgccgatt gccttggcca taatcatcac
     4681 ttttgtcaga agtttgtatg aactttatac caataccggt cccggctatt tcgatagtat
     4741 gaccggaata gtcttattta tgctcaccgg acggattttg caggacagaa cctaccaatc
     4801 gctttctttt gatcgtgatt ttaaatcctt cttccctatt accgtcaatg taataaaaga
     4861 caatctttcc gcaccaacca cattagacaa gctgaaatac ggcgatgtat ttgaagtatt
     4921 ttccaatgag ctgattcctg ttgatgctat attaactaaa ggaaatgctg aaattgatta
     4981 cagttttgta acgggcgaaa gtattcccgt acataaacaa attggtgaaa ttatatatgc
     5041 aggcggaaaa caaataccgg gtaaaattga gctgatggtg gtaaaagaag ttacccagag
     5101 ttatctaacc aatttatgga ataaatcggc tttcaaaaaa tcagatgaat acaaagattc
     5161 ttatgttgat tttatcggca aatatttcac ctggattgtg tttggtattg ctttaatttc
     5221 ctgtacctac tggcttttgc accatgaata taagctggcg ttcaatgtac tgactaccat
     5281 actgatcgtc gcatgtcctt gtgctgtcgc acttgctgcg ccacttgtgt attcgattgc
     5341 ccttgatagg ttggctagca aggatctcta tttagagtct gtcgatgtct tcacaaaaat
     5401 taagaaaatc aaaaatcggc tttaacagat ttgtagagc
//
LOCUS       DAOQII010000171         5426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8718_length_5426_cov_5.438093, whole genome shotgun sequence.
ACCESSION   DAOQII010000171 DAOQII010000000
VERSION     DAOQII010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8718_length_5426_cov_5.438093"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3523)
                     /locus_tag="PLF48_08500"
     CDS             complement(<1..3523)
                     /locus_tag="PLF48_08500"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNF49404.1"
                     /translation="MKKHQPTNWLISVFLILFGLMHSTYASAQVTEFKKLSDEDQRVK
                     DILHQYDQKLFFTQNKGQWDNMVLYKVDFKFGQAIATQNGMLMGTYDPKSMSDIYEWA
                     DKGEENMHKGIPFNEEKPELKGHNWLMHFLQASPKMTIEAKEAHEDKFSYFMEREESK
                     YAALSNNYQEIWYKNVYDKVDVRYYPSEEGHLEYDIICKPGFSKEQIAVQFDGIDNME
                     VDDKGNLVLKTSVQDVQLPAPYAYQVINGVKNKIEIYYTIEKNVLTFQIGVFDKSAPL
                     IIDPIALRWASWLTNNSAGDNHGHGVWVDETDGAIYVLARIVGNGLITSVGAYQTSSA
                     GNLDIVVGKYLEPSAVNQSGTRVWQTYLGGSSDDNPYAMEMGEDGNIYITGYTNSSNF
                     PLLGGTAFNGTGASLDNRSQSASNIFVTKINKAGNSIKSAVIGGNGGGEQSYDLRTTK
                     TDVLVCGQTNSTNLATLYPGSGATNSNAGGTDVLIFKINQNLTAISWMSNYGGSGDDR
                     ANIMLSNPINGDIFVAGQTASSNFPTLNARQTSRGGSQAGFLQKISLSGVTAWRSYFS
                     SASSASASILCMEFNMTKDKLYFGGLTTGLAAANISSSGVYDNSLNGGTDLFVCRMDT
                     GQLFNMSTYLGGTSSEDNMMGLNTDANNDVYVFAYTPSTNFPVTADALQSSNQGQSDK
                     TFSKLNANLNALLFSTYYGGTSEDYDPVGERGIKFANCRIYTLVTAQSNNIPLTQNAL
                     TTNKLSSTSIYEPGIVIWANPPDLLGNSITPNQQICLGATPANMTGSVPAYSVPTIVR
                     NGITYTYPPITTPITYQWQISTDSINWTDISGATGQNLNGNMIGPVFQNTYIRRIISG
                     DACVLSGASDQIISLKILALSGAVTPVTCAPDRNGAINLTVTNGTVPYSYLWNDGVIT
                     KDRSGLSAGTYSVTVTDAHGCSAVKSFDVVNSSTKPEIVATAVPPILCNGQSSILSFS
                     PANDIQTVNWYNGCGTLGAFLGNANTVTVSPSINSQYSVIVQSTSGCKDTACVSVMIE
                     NTPPLITNVPSNLNVSCSSDVPQADISTITVTDNSSGNITVTVQDVISNQTCANRYTI
                     TRTWTATDVCGNSSTSTQIITVDDQTAPSITGTPQNITVTCASDVPTATISAVTATDN
                     CAGAVSITVQDAISNQTCANRYTITRTW"
     gene            4117..4398
                     /locus_tag="PLF48_08505"
     CDS             4117..4398
                     /locus_tag="PLF48_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49405.1"
                     /translation="MQTKKLLLMAICLSVFTAFAKPPQGNMPNLGNEGNHYCWQNNKG
                     CSGQHRQDEHHHGGHHKKKGGFHGQYNGNSQNHQNDKPLDDKNKGQNFK"
     gene            4501..>5426
                     /locus_tag="PLF48_08510"
     CDS             4501..>5426
                     /locus_tag="PLF48_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF49406.1"
                     /translation="MQTTIIRNGIYFDGLGNKGIKKDIIIKGNTIADVLDKAPFIEHA
                     KEIDATDHWITPGFIDLHTHYDAEVEVMPSLDESVRHGVTTVVFGNCSLSAALGKDKE
                     IVDLFARVENIPAKILSDWIIGNIKWTNVKEYYEHLETLPLGPNIATFLGHSNLRIDA
                     MGLERAFLEPKANEQELEQMQKRLEAALAVGYLGLSIDMLPFHRWAGVYDKKYLGISV
                     PSQRAAKSEYFFLADILRNTDSVLQITPNAVDKTSFVTIMRLSQGGFFKKSLKTSIVA
                     ALDLKSNERIYKLLKVLGFIGNDILGAKMMFQT"
BASE COUNT         1644 a         1043 c          944 g         1795 t
ORIGIN      
        1 tccaagtgcg tgttatcgta tagcgattag cacaggtttg attgctgatg gcatcctgta
       61 ccgttatgct tactgctcct gcacaattat ctgttgccgt tactgcgctt attgttgctg
      121 tgggtacatc gcttgcacat gtcaccgtta tattttgtgg tgtgcctgtt atagatggtg
      181 cagtttgatc atctactgtt attatctgag tggaagttga tgaattgcca catacatcag
      241 tggctgtcca agtgcgtgtt atcgtatagc gatttgcaca agtttgattg ctgattacat
      301 cttgtactgt tacagtaata ttgcccgaag aattatctgt aactgttatt gtacttatat
      361 cagcttgtgg cacatcactt gaacaactta catttaagtt tgaaggcaca ttagttatta
      421 aaggcggtgt gttttcaatc attacagaaa cacaagcagt atctttacaa cctgaggttg
      481 attgtacaat tacactatat tgactattta tagaaggtga gacagtaact gtattcgcat
      541 ttccaagaaa tgctcctaaa gtaccacatc cattatacca attaactgtt tgtatgtcat
      601 ttgccggact aaaactcagt attgaagatt gtccattgca tagtattggc ggaacggctg
      661 tagcaacaat ttctggtttt gtactcgaat ttactacgtc aaatgattta actgcactac
      721 atccgtgtgc atcagttacc gttacactat acgtacctgc cgataatcca cttctgtctt
      781 ttgtaattac tccatcattc cataaataag aataaggtac tgttccattt gttactgtca
      841 gattaattgc accattccgg tctggtgcgc aagtgacagg agtaacagca ccactaagtg
      901 ccaaaatttt taacgatata atttggtctg atgcaccgct tagcacacat gcatcgccac
      961 taattattcg tctgatataa gtattttgga ataccggacc aatcatattg ccattcaaat
     1021 tttgtcctgt tgctccagaa atatctgtcc aatttatact atccgtacta atttgccatt
     1081 gatatgtaat cggtgttgtg ataggagggt aagtataagt gattccattt ctaacgattg
     1141 tcggaacgga ataagcaggt acagaaccag tcatattagc cggtgtagcc cctaagcaaa
     1201 tttgttggtt tggcgtaatt gaatttccta acagatctgg aggatttgcc caaatgacta
     1261 tacccggttc ataaattgaa gttgaactta atttattagt tgttaaggca ttttgtgtaa
     1321 gtggtatatt gtttgattgt gctgtaacta acgtatatat tctacaattt gcaaacttga
     1381 tacctctttc tcctaccgga tcataatctt ctgaagtacc tccataataa gtactgaata
     1441 ataatgcatt caaatttgca tttaattttg aaaatgtttt atcggattgc ccttgattac
     1501 tgctttgcaa agcgtctgct gtaacaggaa aattagtact cggcgtataa gcaaaaacat
     1561 aaacatcatt attagcatcc gtatttaaac ccatcatatt gtcttcagat gaggtacctc
     1621 ccaaataagt tgacatatta aatagttgtc ctgtatccat tcgacataca aataaatctg
     1681 tacctccgtt aagtgaatta tcgtaaacac ctgaactaga gatattcgca gcggccaatc
     1741 ccgtagttaa ccctccgaaa tatagtttat cttttgtcat attaaattcc atacacaaga
     1801 tggatgcgct ggcactgctt gccgagctaa aataggatct ccaggcagtt acacctgaca
     1861 aacttatctt ttgcaaaaat ccggcttgag aaccacctct ggaggtttgt ctggcattga
     1921 gggtaggaaa attagatgaa gctgtctgac ctgctacaaa aatatctcca ttaataggat
     1981 tgctaagcat aatattcgcc ctgtcatccc cagaaccgcc ataatttgac atccaagaga
     2041 tggcagtaag attttgattt attttaaaaa tgagtacatc tgttccgccg gcattagaat
     2101 tagtagcccc cgagcccgga tatagcgtag caagatttgt gctgttagtt tgaccacaaa
     2161 ctaaaacatc tgttttggta gtacgtaaat catatgattg ctcgcctcca ccatttcctc
     2221 ctattacggc actcttaatt gaatttcctg ctttattgat tttggtaaca aaaatattgg
     2281 aggcagattg acttctgtta tctaaacttg ctcccgtacc attgaaagca gtacctccca
     2341 acaacgggaa attagatgaa tttgtataac ctgttatata aatattgccg tcttcaccca
     2401 tttccattgc ataaggatta tcatcactac tacctcctaa ataagtttgc catactcttg
     2461 ttccgctttg attcacagct gacggttcca aatatttccc tactactata tccagatttc
     2521 ctgcgctgga agtttgatat gcgccaaccg aagttattaa tccattacca acaatacggg
     2581 ctaatacata tattgcgcca tccgtttcat ctacccaaac accatggcca tggttatctc
     2641 ctgcagaatt atttgtcaac caacttgccc atcgcaatgc tatcgggtca ataattaatg
     2701 gtgctgattt atcaaaaaca ccaatttgaa aggtcaagac atttttttca attgtataat
     2761 atatttctat tttattttta actccgttaa taacttgata tgcataaggg gccggaagtt
     2821 gaacatcctg cacactggtt ttcaaaacaa gatttccctt gtcatcgact tccatattat
     2881 caataccatc aaactgaaca gcaatttgtt ctttactgaa tccgggctta caaatgatat
     2941 catattccaa atggccttct tctgaaggat aataacggac atctacttta tcatatacat
     3001 ttttatacca aatttcctga taattgtttg acaaagctgc atacttggac tcctctcttt
     3061 ccataaaata actaaacttg tcttcatgtg cctcttttgc ttctattgtc atttttggag
     3121 aagcttgtaa aaaatgcatt aaccagttat gcccttttag ttctggtttt tcttcattaa
     3181 atggaattcc tttgtgcata ttctcttctc ctttatctgc ccattcatag atatccgaca
     3241 ttgatttcgg gtcgtatgtt cccatgagca tcccgttctg agtagcaatt gcctgaccaa
     3301 atttaaaatc aaccttatat agaaccatat tatcccactg tcctttattt tgtgtgaaga
     3361 agagtttttg atcatattga tgaaggatat cctttacccg ctggtcttca tcacttagtt
     3421 ttttaaattc agttacttgg gcagatgcat aagtactatg cattaatcca aagaggataa
     3481 gaaatactga aatgagccaa ttagtaggct gatgtttttt caagaatctt aatttgattc
     3541 ctttactgaa gcaatacact gctgtcagga aacacaatgg ttttctcata gttagatagt
     3601 ttttggtttt agcttaagct gtttttgaag cagctttttg ggtatttatg tctaaagact
     3661 ttagaatctt cagttgtctt tgttatgatg gattaaaagt ataactttat tatgtgctct
     3721 atcagatgac ttagtaagtg gttcatttta cttagtaagc ggtaatattc agctgttttt
     3781 cggatttgac ctacatcaaa atataccaaa tattactatt ttaatctaat tagtttaaaa
     3841 tccaatcaaa atacacacaa ccacttaaca ttcaataact taatacaata aatcacatta
     3901 atgtttattt tcatgtgata aatattacaa gatttagcat tgttctttta tttgcatttg
     3961 ataaaatcag ttgtatttat caatctgatt tcagcttggc tgcatagaaa tatgcttgca
     4021 aacttgtaat tctaaaaaat gttagtttat catgtattca gctatctttt aaaaatctgg
     4081 catgctattt tcataactta agaaaaacta attatcatgc aaactaaaaa attattatta
     4141 atggcaattt gcttaagtgt gtttactgca tttgcaaaac ctcctcaagg caacatgcct
     4201 aatcttggca atgaaggaaa tcattactgc tggcaaaaca acaagggatg ttccggacaa
     4261 catcggcaag atgaacatca ccacggtggc caccataaaa agaaaggtgg atttcatggt
     4321 cagtacaatg gcaattcaca aaatcatcaa aatgacaaac cgcttgatga taaaaacaaa
     4381 ggacaaaact ttaaatagcc tttcacctga aaacaacttc ttaaaaaatg cgaggttaat
     4441 ccctcgcttt tttctttttt atactttgcg tatcttgtca gggataataa aaattgaatc
     4501 atgcaaacta ctatcatacg caacggaatt tattttgacg gattgggtaa caaaggcatt
     4561 aaaaaagaca tcatcataaa aggcaacacc atagctgatg ttttagataa agcccctttt
     4621 attgaacatg caaaggaaat tgatgcaact gaccattgga ttacaccggg ttttattgac
     4681 ctgcatacac attatgatgc agaagtagaa gtgatgccct ctttagatga atcggtgcga
     4741 catggcgtaa cgacggtagt atttggcaat tgctcactat cagccgcact tggaaaagat
     4801 aaagaaatcg tggatttatt tgcccgcgta gaaaatattc cggcaaaaat tttgagcgac
     4861 tggataatcg gcaatatcaa gtggacgaat gtaaaagaat attacgaaca cttagaaaca
     4921 ttaccgcttg gtcccaatat agccacattt ttaggtcatt ccaacttgcg cattgatgcg
     4981 atgggcttag agcgggcatt tcttgagcct aaggcaaatg aacaagaact ggaacaaatg
     5041 caaaaaaggt tggaagcagc attagcagtt ggttatctcg gattgtctat tgatatgctg
     5101 cctttccatc gttgggccgg agtgtacgac aaaaaatatt taggcatttc cgttccatca
     5161 caacgtgccg cgaaatctga atactttttc ctggcagaca ttctccgaaa taccgacagc
     5221 gttttacaaa ttacacctaa tgcggttgat aaaacttctt tcgtaactat catgcgttta
     5281 agccaaggtg gtttttttaa gaagagtttg aagacttcca tcgtggctgc attagattta
     5341 aaatcaaacg agcgcattta taaattgctg aaagtgctgg gcttcattgg caatgatatt
     5401 ttgggtgcta aaatgatgtt tcaaac
//
LOCUS       DAOQII010000172         5416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8743_length_5416_cov_5.213953, whole genome shotgun sequence.
ACCESSION   DAOQII010000172 DAOQII010000000
VERSION     DAOQII010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8743_length_5416_cov_5.213953"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1311)
                     /locus_tag="PLF48_08515"
     CDS             complement(259..1311)
                     /locus_tag="PLF48_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNF49407.1"
                     /translation="MTIDDLKSQNLILFECISGSKAYGLDTATSDTDIKGVFLLPKED
                     FYGLNYIPQVSNETNDIVYYELGRYFELLLLNNPNILELLSTPENKILYKHPFLNAIN
                     AEMFLSKLCFNTFGQFAISQIKKAKGLNKKILNPIEAEKKNILNFCTINYNQGSISLN
                     KFLEIKNWTQNNCGLVNIPNMKNVYGLYYSDKIKYNGIIRDENSNEVLLSSIPKEEKQ
                     EAILYFNKEGYTTYCKEYKEYWSWVGKRNTTRYENTLSHGKNYDAKNMMHVLRLLDMA
                     IEIANEKKIFVKRPNRDFLLMVKSGELEYDEIMEMANKKKQEMEMAFENSSLPDMPDV
                     TILKKILVDLRCKFYA"
     gene            complement(1316..2074)
                     /locus_tag="PLF48_08520"
     CDS             complement(1316..2074)
                     /locus_tag="PLF48_08520"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNF49408.1"
                     /translation="MEKLILSYLNEIESKYEIEILLACETGSRAWGFPSPDSDYDVRI
                     IYKHNVDWYLSLSEKKDYIEWMAEGNILDITGWDLRKTLRLLYKSNASILERIQSPII
                     YKHNEEFLSQINELAQSCFSPIATFYHYFGLAKNTFSDLDGCKELKLKKLFYALRATL
                     ACNWIIQKDSIPPISFITMVNELAFDENIRAEIKNLITLKLTKDESYIHQADENMLQF
                     IRENLNAATADAARLTGAKNKQTDLDRFFKNWIK"
     gene            complement(2239..>3546)
                     /gene="cysN"
                     /locus_tag="PLF48_08525"
     CDS             complement(2239..>3546)
                     /gene="cysN"
                     /locus_tag="PLF48_08525"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNF49409.1"
                     /translation="TNDQSTNAQLNNEHFDSKAYLDMELLRFTTAGSVDDGKSTLIGR
                     LLYDSKSIFEDQLEAIESASEKRGEDYVNLALLTDGLRAEREQGITIDVAYRYFSTPK
                     RKFIIADTPGHIQYTRNMVTGASTANLAIILVDARKGIIEQTFRHSYIASLLQIPHIV
                     VCINKMDLVEWDEKTFNKIVHDYKAFASKLDVKDVHFIPISALNGDNVVDRSTNMNWY
                     NGPTLLYLLENIHIGSDLNHIDARFPVQYVIRPMKNEYHDYRGYAGRIAGGVFRVGDR
                     VKALPSGFTSKIKSIKTMDGDLDEAFAPQSVTLLLEDEIDISRGDMIVRENNVPHVEQ
                     EFDVMLCWMNDKKLVPRGKYILRHTSKECKCIVKEIKYKLDINTLTRLEGEIEIGLND
                     IGRVSLRTTTPLFFDAYRKNRNTGSIILVDEATNETIAAGMIV"
     assembly_gap    3547..3646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3652..4029)
                     /locus_tag="PLF48_08530"
     CDS             complement(3652..4029)
                     /locus_tag="PLF48_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF49410.1"
                     /translation="MGIEELGVYNKALAFLNKIWEIIIPLSYFEKNTIGNQICRSADS
                     ISANIADGFGRYYYKDSKLFYYYARGSVFETKDWLHKLKDRNIINEITYTMLIDELNS
                     IGKMLNNYIKSIGKLNQTTTNDQ"
     gene            complement(4124..5029)
                     /gene="cysD"
                     /locus_tag="PLF48_08535"
     CDS             complement(4124..5029)
                     /gene="cysD"
                     /locus_tag="PLF48_08535"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNF49411.1"
                     /translation="MTDYHLNHLRELESESMYVLREVAAQFENPCILFSGGKDSIVVT
                     HLAIKAFYPASVPFTLLHVDTGHNFQETLDYRDTLVQKNGLRLLVASVQDAINKGLVR
                     EEKGYNASRNALQTAPLLEAIEVYKFDCAIGGARRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNLFNGRKHVGEHFRVFPISNWTEMDVWQYIYQEKIEMPSLYFSHKRNVFL
                     RDGVWYADSEYMQKKPDEVVEEQIVRFRTIGDITCTGAVFSTASTLEDIIQEVAASRT
                     TERGTRADDKRSEAAMEDRKKAGYF"
     gene            complement(5062..>5416)
                     /locus_tag="PLF48_08540"
     CDS             complement(5062..>5416)
                     /locus_tag="PLF48_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49412.1"
                     /translation="IMDEYGIKISKWYEIKYFLIGKCISWSCYVMGWFMPMYFAGRLE
                     SGNVNEYYRMKELFNSVSITKYDEILVEMGIKEKEHEIYFLNKIKQHKWLPCFEKIFN
                     WGNNKSFNTINLNEE"
BASE COUNT         1585 a         1013 c          808 g         1910 t
ORIGIN      
        1 ttaacaaggt ggaggaatta aaaggaggtg gttctacata aaaaatcaac tatttattaa
       61 ttgaaatgat tatcccgaac ttaggttaat agaattttaa tttccattgt aattgttaaa
      121 tacttcgcat taaacattgt agataaatta gcttttatgc caaattcaat cataacaaga
      181 aaaggcaaaa ccatttcata atactgctta acaaaattga aatagttctt actatagaac
      241 tataataata tctcacaatt aagcataaaa cttacacctt aaatcaacta atattttctt
      301 taaaattgtt acatccggca tgtcaggaag gctagagttt tcaaatgcca tttccatttc
      361 ttgctttttt ttattagcca tttccataat ttcatcatac tccagctccc ccgattttac
      421 cattaataaa aaatctctat tcggtcgctt cacaaatatt ttcttttcat ttgcaatttc
      481 aatagccata tctaataacc ttaaaacatg catcatattt tttgcgtcat agtttttgcc
      541 atgacttagt gtattttcat atcgtgttgt atttctttta cctacccaac tccaatactc
      601 tttatattct ttacaataag tggtgtaacc ttctttgtta aaatataaaa tcgcttcttg
      661 tttttcttct tttgggattg aagacaaaag cacttcatta gaattttcat ccctaattat
      721 tccattgtat tttattttat ctgaataata gagtccatac acattcttca tgtttgggat
      781 attaactaac ccacagttat tttgagtcca attttttatt tcaagaaatt tattcagact
      841 aattgagcct tgattatagt ttattgtaca gaagttgaga atattttttt tttctgcttc
      901 aattggatta agaatttttt tattaagtcc ttttgccttt tttatttgag aaatagcaaa
      961 ttgtccaaag gtattaaaac acaattttga gagaaacatc tcagcattta ttgcattcaa
     1021 aaacggatgt ttataaagaa ttttattttc aggtgtagat aaaagctcca agatatttgg
     1081 gttgttcagt agtaataatt cgaagtatct tcctaattca taataaacaa tatcatttgt
     1141 ttcattactt acctgaggta tataatttaa tccgtaaaaa tcttctttag gcaataaaaa
     1201 tacaccctta atatccgtat ctgaagttgc agtatccaaa ccatacgctt tacttccgct
     1261 aatacattca aacagaatta agttttggga ttttaaatcg tcaatggtca tttttctatt
     1321 ttatccaatt tttaaaaaat cgatccaaat ctgtttgttt gtttttagcc cctgtcaatc
     1381 tcgcagcatc tgctgtagct gcatttaaat tctctctgat aaattgaagc atattttcgt
     1441 cagcctggtg aatataagat tcatctttag taagcttcaa tgtaatgaga ttcttaattt
     1501 cagctctaat attttcatcg aaagctaatt catttaccat tgtaataaaa ctaattggcg
     1561 gaattgaatc tttttggata atccaattac aagctaatgt tgcacgaagt gcataaaata
     1621 attttttgag tttaagttct ttacatccat ctaaatctga aaatgtattt tttgctaaac
     1681 caaaataatg atagaacgtg gctattggtg aaaaacaaga ttgagctaat tcattaattt
     1741 ggcttaaaaa ttcctcattg tgcttataaa ttattggcga ctgtattctt tctaatatag
     1801 aagcatttga tttatataac aatcttaatg ttttacgtaa atcccatcca gttatatcca
     1861 atatgtttcc ttctgccatc cattctatgt agtctttttt ttcagaaaga gataaatacc
     1921 agtctacgtt gtgtttataa ataattctta catcataatc actatcagga gatggaaacc
     1981 cccatgctct tgaacctgtt tcacatgcca atagtatctc aatttcatat ttactctcaa
     2041 tttcatttaa atacgataaa attaattttt ccataaatgt aaatatataa aaataattta
     2101 aaaatcaaaa cgcttattta ttgatggaac aaattaagtg atagaagact actgaatgaa
     2161 gcaatacaaa aacccattgc cttatttgct tagtaacaaa taaaaagaat ctacaatcaa
     2221 accactaatt tacaaaactt atactatcat ccctgctgcg atggtttcgt tagtagcttc
     2281 gtctaccaag atgatacttc ccgtatttct gtttttgcga taggcatcaa aaaataaagg
     2341 tgtggtcgta cgtagcgata ctcgacctat atcattcaat ccaatttcaa tttctccttc
     2401 taatctggta agggtgttga tatccaattt gtatttaatt tctttcacga tacacttgca
     2461 ttctttggag gtatggcgca aaatatattt acctctcggc accagttttt tgtcattcat
     2521 ccagcatagc ataacatcga attcttgttc tacatgtggc acattgtttt cgcgtacaat
     2581 catgtcacca cgtgaaatat cgatttcatc ttccagcaac aaggttacac tttgcggggc
     2641 aaatgcctca tccaaatcgc catccatagt tttaatggat tttatttttg aagtgaatcc
     2701 acttggcaag gctttcactc tgtctcctac cctgaaaacg ccgcctgcaa ttctgcctgc
     2761 ataaccgcgg taatcgtggt attcattttt catcggacga attacatatt gtaccggaaa
     2821 acgtgcatct atatgattca gatccgagcc gatatggata ttttccaaca agtataaaag
     2881 tgtgggtccg ttataccagt tcatgtttgt gctgcggtca acaacgttat cgccattcag
     2941 tgcagaaatt ggaataaaat gcacatcttt tacatctaat tttgatgcga aggctttgta
     3001 gtcatgcaca attttattga atgttttttc atcccattct accaaatcca tcttgttaat
     3061 acagactacg atatgcggga tctgcaacaa ggaggcgata taagaatggc ggaaagtctg
     3121 ctcaattatt ccttttctgg catccactaa tatgatggca agatttgccg tagaagcacc
     3181 ggtcaccatg ttgcgggtgt attggatatg tccgggcgta tcggcgataa taaattttct
     3241 ttttggcgta gaaaaatagc ggtatgccac atctatcgtg attccttgtt cgcgttcagc
     3301 acgcaagccg tcggtcaaca atgccaggtt tacatagtct tcgccacgtt tttcagatgc
     3361 tgattctatg gcttcgagct ggtcttcgaa gatggactta ctgtcataca gtaatcttcc
     3421 gattaacgta gattttccgt catcaacaga tcctgctgtg gtaaaacgca gcagttccat
     3481 atccagatat gccttactgt caaagtgttc attgtttagt tgggcattgg ttgattggtc
     3541 attggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattg gtcattggtc
     3661 attggtagta gtttgattaa gttttccaat tgattttata tagttgttta gcatctttcc
     3721 tatgctattt aactcgtcta ttagcattgt gtacgttatt tcattaatta tatttctatc
     3781 ttttagttta tgaagccagt ctttagtttc aaatacagaa cctcttgcat agtagtaaaa
     3841 tagcttactg tccttataat agtagcgacc aaatccgtca gctatatttg ctgaaataga
     3901 atcagctgat ctgcatattt ggttaccaat tgtatttttt tcaaaataag acagaggtat
     3961 tattatttcc caaattttat ttaaaaatgc taatgcttta ttatacaccc ctaattcttc
     4021 tattcccatt ttctgtctaa tctatttatt tcaactcaaa aaacatacga aattagttat
     4081 acatttatca ccaaataatc actaatgact aattaaccaa ttactaaaaa tatcctgcct
     4141 ttttacggtc ttccatggcg gcttcagagc gtttgtcatc ggcgcgcgtg cctcgttcgg
     4201 ttgtacgact tgctgccact tcctgaataa tatcttctaa agtagatgct gttgaaaaca
     4261 ccgcacctgt acacgtgata tcgccgatag ttctgaaacg cacgatttgc tcctcgacca
     4321 cttcatccgg ttttttctgc atgtattcgc tatcggcata ccatacgcca tcccgcaaaa
     4381 aaacatttct tttgtgtgaa aaatacaaac tcggcatttc aattttttcc tgatatatat
     4441 attgccacac gtccatttct gtccagttgg aaatcgggaa cactcgaaaa tgctcgccta
     4501 catgttttct gccgttaaac agattccaca gttcgggacg ctgattttta ggatcccatt
     4561 gtccgaattc atcacgatgt gagaagaatc tttctttggc acgtgctttc tcttcgtccc
     4621 tgcgtgctcc gccaatagca caatcgaatt tatacacttc aatagcttca agcagtggtg
     4681 ctgtctgcaa tgcattgcga gaagcattgt atcctttttc ttcacgcacc agacctttat
     4741 tgatggcatc ttgcacagat gctaccaaca aacgcagtcc gtttttttgc actaaggtat
     4801 ctcgataatc aagtgtttcc tgaaaattgt gcccggtgtc cacatgcaat aatgtaaagg
     4861 gcacagatgc cggataaaat gctttgatgg caagatgtgt aacgacgata gagtctttac
     4921 cgcccgaaaa caggatacac gggttttcaa attgtgccgc cacttcacga agtacataca
     4981 tggattcgga ttctaattcg cgcaagtgat ttaagtgata gtcggtcata ttacttactt
     5041 attagtgatt ggtgattagt gttattcttc attaagattg atagtattaa agcttttgtt
     5101 gtttccccag ttgaatattt tctcaaagca tggcagccat ttatgttgtt ttatcttgtt
     5161 taaaaaatag atttcatgtt ctttttcttt tatgcccatt tcaactaaaa tttcatcgta
     5221 cttagtaata gaaacggaat taaaaagttc tttcattcta taatattcat ttacatttcc
     5281 gctttctaat ctccccgcaa aatacatggg catgaaccaa cccatcacat agcaactcca
     5341 gcttatacat ttgccaatca ggaaatattt tatttcatac catttcgaga ttttaattcc
     5401 atattcatcc attatt
//
LOCUS       DAOQII010000173         5415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8747_length_5415_cov_5.111007, whole genome shotgun sequence.
ACCESSION   DAOQII010000173 DAOQII010000000
VERSION     DAOQII010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8747_length_5415_cov_5.111007"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="PLF48_08545"
     CDS             <1..1440
                     /locus_tag="PLF48_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNF49413.1"
                     /translation="KLGKILLATVKGDVHDIGKNIVGVVLACNNFEIIDLGVMIPADK
                     ILDEAQKQNVDIIGLSGLITPSLDEMVDVAKEMERRGMKTPLLIGGATTSKLHTAVKV
                     EPHYSGATIHVLDASRSVPVATQLMNKADALQLEKDIRAEYEVMRDRHNNKGVIKDYI
                     SIEEARKNKTPIDWTTYSPPKPTFLGVKVFDDYDLSELRNYIDWTPFFQAWELHGKYP
                     AILEDKIVGDEAKKLFADANAMLDKICTEKWLTAKAVIGFFKANTISDDDIEIVADNK
                     IKVVHHLRQQNKKAEGLPNISLADFIAPVQTQKEDYIGAFAVTTGIGIEAKIKDFEEK
                     HDDYNAILLKVLADRLAEALAERMHERVRKEFWAFDKNEKLTNDELIKEKYTGIRPAP
                     GYPACPDHTEKITLFDILNATQNAGITLTESMAMYPTAAVSGWYFSHPDSKYFGIGKI
                     SKDQVESLAKRKEMTTESVEKWLGSVLNY"
     gene            complement(1642..2274)
                     /locus_tag="PLF48_08550"
     CDS             complement(1642..2274)
                     /locus_tag="PLF48_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49414.1"
                     /translation="MIVYPGGTFMHTEYTHYYFLENFFSDLGRTQTPNGSMNLFSLIC
                     FDTALIFQAVALYVFNIQFLKEVKAKELHTVFYYIAIVCGILFPVFLYGIAITPCNLF
                     LKAHMFCVRWGFGILVPLSLAYTILVRKHHLLPNKYGNVMQVIVLACFLYFILIMFGP
                     NTRENIYIQPVAQKVIVYAMVFSLVYLTSGCMRYLHIPTKQEIREAQESA"
     gene            complement(2366..3634)
                     /gene="glyA"
                     /locus_tag="PLF48_08555"
     CDS             complement(2366..3634)
                     /gene="glyA"
                     /locus_tag="PLF48_08555"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNF49415.1"
                     /translation="MERDKKIFSLINDELHRQTVGIELIASENYTSKQVMEAMGTCLT
                     NKYAEGYPGKRYYGGCEIVDQIEQLAIDRLRTLFGAEYANVQPHSGAQANAAIFLACL
                     KPGDVVMGFNLSHGGHLSHGSPVNFSGKHFRPVFYGVEQETGTIDMDKVEALALQEKP
                     KLIICGASAYARDWDYKRFRAIADQVGALLLADIAHPAGLIAKKKLNDPMPHCHIVSS
                     TTHKTLRGPRGGIIMLGKDFENPWGEKTLKGETKMMSVILNSAVFPGMQGGPLEHVIA
                     AKAVSFGECLTDEFGQYADQVMLNARVMANEFMKRGYNLVSGGTDNHLMLIDLRNKNI
                     SGKDAEKALVQADITVNKNMVPFDDKSPLVTSGIRIGTPAITTRGLNEQDCLQVVDWI
                     DTILKAPTNVALIADIKGEVNKFMSDFPLY"
     gene            4194..5408
                     /locus_tag="PLF48_08560"
     CDS             4194..5408
                     /locus_tag="PLF48_08560"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HNF49416.1"
                     /translation="MDFIDFLREMLGITKDYGIIKIEKIEHPEKIIRIYLSYTSSVYK
                     SGTSTYPLYDLAQEREWQHLSWFEYKCYLVCRLPRYVDKDKKVKTVVPDFASPGKSYT
                     HLFSHVAIQYLQKVRVQKTVAELMQTSPYIIRSIMEHAVEAALETRGFVTNLEQISID
                     EKAYAKGHEYATIVIDSKREYVVDMQEGRKEKSLKSLLYTITGEETQPQLRRVNIDMW
                     QPYMKVIEELAPRALVVHDKFHLIKKLSEAIDKTRRKEISESGLLKRQKYTVLKNADN
                     WTKEQKSCFEQIERANLKTAQAWHVRENFKLLFQWRCTQQAEYLLKEWMQQSREKGIF
                     HVNRVVATFEAHLNGIINAITTKTDSSMHENMNGKIQSVIAKAKGFINFERFRINILF
                     YFGNLQLLPQKI"
BASE COUNT         1861 a          980 c         1021 g         1553 t
ORIGIN      
        1 aaattaggta aaatattatt agccaccgtg aagggtgatg tgcacgatat tggtaaaaat
       61 attgtaggcg ttgtactggc ttgtaataat tttgagataa tagatttagg cgtgatgata
      121 cccgccgata aaatattaga tgaagcacaa aaacaaaatg tggacatcat tggtttgagc
      181 ggattaatta cgccatcttt agatgagatg gtcgatgtag ccaaagaaat ggagcgcaga
      241 ggcatgaaaa cgcctttatt aattggcgga gctactacat ccaaattgca tactgccgta
      301 aaagtagaac ctcactattc aggtgctacc atacatgtat tagatgcctc tcgaagtgtt
      361 cctgttgcta cacaattgat gaataaagca gatgcgctgc aactggaaaa agacatacgg
      421 gcagaatacg aagtgatgcg cgacaggcac aataacaaag gcgtgattaa agattatatt
      481 agcatagaag aagcgcgcaa aaataaaact ccgatagact ggacaactta ctcaccgcct
      541 aagcctactt ttttaggtgt taaagttttt gatgattacg atttaagcga attaagaaat
      601 tatatcgact ggacgccgtt ttttcaggct tgggaattac acggcaaata tccggcaata
      661 ttagaggata agattgttgg agatgaagcc aaaaaattat ttgcagatgc gaatgctatg
      721 ctggataaaa tttgtacaga aaagtggttg acagctaaag cagtaatcgg cttctttaaa
      781 gccaatacca tcagcgatga tgatatagaa attgtagcag acaacaagat aaaagtagta
      841 catcatttgc gccagcaaaa caaaaaagcg gaaggcttgc caaatatttc tttagccgat
      901 tttattgcac ctgtacaaac acaaaaagaa gattatatcg gtgcttttgc tgttacaact
      961 ggcattggca tcgaagctaa aattaaagat tttgaagaaa aacatgatga ttataatgcc
     1021 atcttattaa aggtactggc agacagatta gcagaagctt tggcagagcg gatgcacgag
     1081 cgtgtgcgaa aagaattttg ggcttttgat aaaaatgaaa agctgacgaa tgatgaatta
     1141 attaaagaaa aatatactgg catacgtccg gcaccgggct atccggcttg ccccgaccac
     1201 acagaaaaaa ttacgttgtt tgacatcttg aatgctaccc aaaatgccgg tataaccctt
     1261 accgaaagca tggctatgta cccaacggca gcggtgagtg gttggtattt ctcacatcct
     1321 gacagtaaat attttggtat aggcaagata agcaaagacc aagtggaaag cctcgccaaa
     1381 cgcaaagaga tgaccactga aagtgtagaa aaatggctgg gaagtgtttt aaattattga
     1441 caatatagta attataaact taaattttat aatgtactaa aataatatat atttaaatta
     1501 gtatttaaaa aaataagcac gctgattaag cagataaaac tgatttacaa agataaaaaa
     1561 aacaatccgt ttatttcatc aatttttcct ttagcctgat tgccagcaaa tatcagaaag
     1621 ctgaactatt cataatagaa attacgcact ctcctgcgct tcacgtattt cttgcttcgt
     1681 cggtatatgc aaataacgca tgcatcccga tgtaaggtag accaatgaaa acaccatagc
     1741 atacacgatt actttttggg ctaccggctg gatataaata ttttctcttg tgttaggacc
     1801 aaacatgatc agtataaaat aaagaaaaca cgccagtaca ataacctgca ttacattgcc
     1861 atatttgttt ggcagcaaat gatgcttgcg tactaaaata gtatatgcca aactaagcgg
     1921 caccaagata ccaaaacccc aacgcacaca aaacatatgg gctttcagaa ataaattgca
     1981 aggtgtaatg gcaattccat ataagaatac cggaaataaa atgccgcaaa ctattgcaat
     2041 ataataaaac acagtatgta attctttggc tttcacttct tttaaaaact gaatattgaa
     2101 aacatataag gctaccgcct gaaaaatcaa tgccgtatca aagcatatta gtgaaaataa
     2161 attcatgctg ccattcggag tttgagttct gcctaagtcg gaaaagaaat tttctaagaa
     2221 ataataatgc gtgtattccg tatgcataaa tgtgcctccc ggatagacaa tcatgcccac
     2281 catagttaat actaaaaaag aaatgcaact tacatagagt aatttaaagc caacgacttg
     2341 gctcttgtta aaatattggc tcatcttaat ataaaggaaa atctgacata aatttattaa
     2401 cctctccttt tatatctgct ataagtgcca cattagtagg agctttcaga atggtgtcta
     2461 tccaatccac tacttgcaaa cagtcttgct cattcagtcc gcgtgtagtg atggctggtg
     2521 taccaatacg aataccggat gtaaccaatg gcgatttatc atcaaacggc accatatttt
     2581 tgtttacagt gatgtcggcc tgcaccaaag ctttttctgc atctttgccc gaaatatttt
     2641 tattgcgcaa atcaatcagc ataagatgat tgtctgtacc accggaaact aaattgtagc
     2701 cacgtttcat aaattcgttt gccatcacac gagcattcag catcacttgg tcggcatact
     2761 gcccaaattc atcagttaaa cattcaccaa aagaaactgc ttttgctgca atgacatgtt
     2821 ctaaaggacc accttgcata cccggaaaca cagcagaatt caaaatgaca gacatcatct
     2881 tggtttcgcc tttcagcgtt ttttctcccc aaggattttc aaaatcttta cccaacatga
     2941 taattccgcc ccgtggccct ctcagtgttt tatgagtagt agatgaaaca atatgacaat
     3001 gtggcattgg gtcgttgagt ttctttttgg caattaagcc ggcgggatgt gcaatatctg
     3061 ccagaagcaa tgctccaact tgatcggcaa tggctctgaa gcgtttgtaa tcccagtctc
     3121 gtgcataagc agatgcgccg caaatgatta atttgggttt ttcctgcaat gccaaagctt
     3181 ctactttatc catatctatt gtgccggttt cttgttctac gccatagaat acgggtctga
     3241 aatgtttacc tgagaaattt acgggagagc catgcgaaag atgacctcca tgcgataaat
     3301 taaagcccat tactacatct cccggtttca aacaggctaa gaagatggca gcattggctt
     3361 gtgcaccgga gtgcggttgc acattggcat actcagctcc aaaaagtgta cgaagcctgt
     3421 cgatagctaa ttgctcaatc tgatctacaa tttcgcagcc gccatagtag cgtttgcccg
     3481 gatagccttc cgcatattta tttgtcagac aagtgcccat agcttccatg acttgtttag
     3541 atgtataatt ttcagaagct atcagctcta tgccaactgt ttggcggtgt aattcatcat
     3601 tgattaatga gaatattttt ttgtcgcgtt ccatgatgtg aaaatactta gtcttacagt
     3661 tttttgaaaa ccctttagaa agatttttat aaaattaatc taaagcagga taggcatatt
     3721 cataatcacc tgaatttagg cctaagttca agtgttaaat gcaatttttt ggggcataaa
     3781 ataacatttt ttcaaaacag agaggaggaa gtttttgata caaaatcaat tttttttaaa
     3841 ttaaaacact tacctcaaac ctaggttagt atttattgaa ataccttttc caaaagccgg
     3901 tcaataaaat gcttttatct ctaaaatgaa aactttttta atcgttttgt aataaaacta
     3961 aattaaattc agaattaaac tggaattatt gcattacttt ttttcgtatg atttgataat
     4021 tatcaatttt ctctcataaa taatcctttt tttagcactt tgattgataa atctcaattg
     4081 atacttgtat atacaaataa atcattgtat ctttgagttc ataacttacc tcttcagtag
     4141 tacttttttg gctaaactaa aaattaagtg gtaaaaattt tatactttcc ggtatggact
     4201 ttatagattt tttaagagag atgctaggaa ttacaaaaga ctatggcatc ataaagattg
     4261 agaaaataga gcatcccgag aaaattatca ggatatatct gagctatacc tcaagtgttt
     4321 ataaatcggg tacatccact tatccacttt atgatttagc tcaggagcgt gaatggcagc
     4381 acttaagttg gtttgaatac aaatgttatt tggtttgtcg tttaccgcgt tatgtagaca
     4441 aggataaaaa agtcaagaca gttgtgccgg attttgcttc tccgggtaag agttacactc
     4501 atttatttag tcatgtcgcc atccagtatt tacaaaaagt acgcgttcaa aagacagtag
     4561 cggaattgat gcaaacgagt ccatatatca ttcgcagtat aatggaacat gccgtggaag
     4621 ccgcattgga aaccagagga tttgttacta atttagagca aatcagtata gacgaaaaag
     4681 catatgccaa aggacatgaa tatgccacca tcgtgataga tagtaaaaga gaatatgtag
     4741 tggatatgca agaagggaga aaagaaaaaa gtttaaagtc cttattatac acgattactg
     4801 gcgaagaaac gcaaccacaa ttaagacgag taaacataga tatgtggcaa ccatatatga
     4861 aagtcataga agagctagcc ccacgggcat tggttgtaca cgacaagttt catctaatca
     4921 agaaattatc ggaggctata gataaaacaa gacgaaaaga aatctcggaa agtggtctgt
     4981 taaaaagaca aaaatacacg gtgcttaaga atgcggacaa ttggactaag gaacaaaaat
     5041 cttgttttga acagatagaa agagcaaatc taaaaacagc ccaggcatgg catgtaagag
     5101 aaaacttcaa actcttgttt caatggaggt gcactcaaca ggcagaatac ttgctaaaag
     5161 aatggatgca gcaatctcgt gaaaaaggga tattccatgt aaacagggtc gttgccacct
     5221 ttgaagcaca tctaaatgga atcataaacg caattacaac taaaacagat agtagcatgc
     5281 atgaaaacat gaatggcaaa attcaatccg taattgccaa agctaaaggc tttataaatt
     5341 tcgaacgctt tagaatcaat atcctctttt atttcggaaa tctccagctt ttaccacaaa
     5401 aaatttagct ttgcc
//
LOCUS       DAOQII010000174         5411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8757_length_5411_cov_4.014190, whole genome shotgun sequence.
ACCESSION   DAOQII010000174 DAOQII010000000
VERSION     DAOQII010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8757_length_5411_cov_4.014190"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLF48_08565"
     CDS             <1..460
                     /locus_tag="PLF48_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNF49417.1"
                     /translation="IFVIPGFGVVGISGILCIIVGLTLSLIDNHNFDFPHGFSETIGY
                     ALFRVTVTLIAGITLAALFGGSIFNFPFFNKMVLKDVQQSDKGFTIKQEDQVEMIGKH
                     GVVISDLRPSGKVEIESNLYDAISDAAFISAGTEVVVTKVQGYSVKVKKA"
     gene            463..1185
                     /gene="kdsB"
                     /locus_tag="PLF48_08570"
     CDS             463..1185
                     /gene="kdsB"
                     /locus_tag="PLF48_08570"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNF49418.1"
                     /translation="MRILGIIPARYASSRLAGKPLAMIAGKSMIQRVYEQCTQAEKLT
                     EVIIATDDERILQHVLQFTDKVIMTSASCRNGTERCAEVVSQYVPEFDFIVNIQGDEP
                     FINPKQIDELCALFDVYKNDIYTQVKPETDEALYSNKNVVKAILDEQTYALDFFRETV
                     ELFVSPFYKHIGIYGFKPQTLLDLVKLPPSQREQQLHLEQLRWLDNGYAIKAGITAYH
                     SMSIDTADDLAKAIKYAVDFQN"
     gene            complement(1343..2161)
                     /locus_tag="PLF48_08575"
     CDS             complement(1343..2161)
                     /locus_tag="PLF48_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HNF49419.1"
                     /translation="MKKLLLLSLLAGIFFIRCQSPREKMIATDTLLLDTIKIDTTDIT
                     CKDAKNENCPNLHFGYPDFKTKDDSINQYLNNETKQILLSHFDSATTSVSAYIQSFFQ
                     LYHQDKLSIHQYDDLPGEYYSTIDITPAYKNKQYYTLVYTSDIFLGGAHPFGSQDYYV
                     YDLSKLKKVVLSDILKTEDENLWKLAEKYFRKELQISDSVSLADAGYFVLGENIEDED
                     SPDYGKFKLSQNFAITEKGILFLYNHYDIAPYAAGRQSFEIPFTELKPYLKIKL"
     gene            complement(2219..2782)
                     /locus_tag="PLF48_08580"
     CDS             complement(2219..2782)
                     /locus_tag="PLF48_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49420.1"
                     /translation="MIGFIAGLITANAFEWHFHKYILHDQGKKKHSMWRYHWHDHHKT
                     VLQQEYRDPAYEKRIFDELNPQSHEVLSLASAAAIVAPLFPLAPGFVSALWLSQYNYY
                     RVHKKSHLDPEWGYKYLPWHYDHHMAPDQDKNWCVTFPLWDYVMGTRLIYKGTEREKL
                     DNERRKRKREQKAAATVQTNTSVVALN"
     gene            2884..4038
                     /locus_tag="PLF48_08585"
     CDS             2884..4038
                     /locus_tag="PLF48_08585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49421.1"
                     /translation="MKFSLTPDYTMTVFSCIAHIQKDDWQSIKKDMQFYQSYNFLQII
                     EALHPELRMRYAFIYKQEKLFGIVYTQQMEFSYRQVFTYSESKENIFIRTIKKYLSGK
                     QVYLLNLGDVFFTGDKGILSENESDLLPLMPEILNQINNTFTENRSGAFLMANITLSD
                     ESKCVFYKSQKYHPFITEPDMYMHIRDEWHSFQDYLNALSAKYRTRAKKVMSTASPII
                     CRALIDEEALLYKNDMAQLYANVMDKTSFNLARLNPDFFIRVRQEYPNNCTLKGYFLE
                     GKMVAFVCLFACNDKQLHIHYIGLNYEVNAQYKLYNRMLFDFLQYAIENGFDKIHFGR
                     TATEIKSTVGAQALQLHAYLKMKNRLMNLILPDMLEKIKPQPYTLRNPFK"
     gene            4151..5383
                     /locus_tag="PLF48_08590"
     CDS             4151..5383
                     /locus_tag="PLF48_08590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNF49422.1"
                     /translation="MKNLLILGAGTSGTMMVNHLNKELYEDDWKLTIVDEREKHYYQP
                     GYLFLPFDIYEPEDIIKPIQEFIPDRAELIRKKIDKVVPENNFVLLADGTKLSYDILI
                     IATGNDIAPEEIEGMKGAEWQKSVFDFYTFEGALALRDKLRTWEGGKLVVHITEMPIK
                     CPVAPLEFSFLADAYFKEKNLRDKVEITYVTPLSGAFTKPKATATLEHLLVEKEIKIE
                     PDFAIEHVDNENKKIVDYGGKEISFDLLVTVPTNKGSEMVARSGLGDELNYISTYKST
                     LQSKKHANIFVIGDATDVPASKAGSVAHFEAEILTENILRFIKGEPLKEEFDGHANCF
                     VETGGGKALLIDFNYTHEPVEGEFPFPGVGPLRLLKESRMNHMAKLAFRWIYWHMLLK
                     GTHIPFIPTQMSEKGKHY"
BASE COUNT         1786 a          900 c          999 g         1726 t
ORIGIN      
        1 aatatttgtg atacccggtt ttggtgtagt tggcataagc ggtattttat gcattattgt
       61 tggactcacc ttaagcctga tagataatca taattttgac ttcccacacg gtttttctga
      121 aacaatagga tatgcactgt ttagagttac agtaacgctt attgcaggca taacgcttgc
      181 tgctcttttt ggtggcagta tttttaattt tccgtttttc aataaaatgg tcttgaaaga
      241 tgtacaacaa tcagataaag gttttaccat caagcaagaa gatcaagttg aaatgattgg
      301 aaaacatgga gtagtaatca gtgatttgcg tcctagcgga aaagtagaaa ttgagagcaa
      361 tttatatgat gccatttcgg atgccgcttt tataagtgca ggtactgaag tcgtggtcac
      421 gaaagtgcag ggctattctg ttaaagtgaa aaaagcataa atatgcgcat cttaggtatt
      481 atcccggccc ggtatgcctc gtcgcgttta gccggtaaac cactggcaat gattgccgga
      541 aaaagcatga tacaaagagt atatgaacaa tgtactcaag cagaaaagtt aactgaggtt
      601 attattgcaa ccgatgatga acgcatactt cagcatgtat tacaatttac agataaagta
      661 attatgacct cagcatcttg cagaaatgga acggaacgat gtgcggaagt cgtttcacaa
      721 tatgtccctg aatttgattt tattgtcaac atacagggag atgaaccatt tatcaatcca
      781 aaacaaattg atgaattgtg tgctttattt gatgtttata aaaatgacat ttatactcaa
      841 gtaaaaccag aaacagatga ggcattatac agcaataaaa atgtggtaaa agcaatttta
      901 gatgaacaaa catatgcctt agattttttc agagaaacag tcgagttatt cgtttcgcct
      961 ttctataagc atattggcat ttatggtttt aaaccacaaa cacttttgga tttggtaaaa
     1021 ttgcctccat ctcagcgaga gcaacaactt cacttggagc aattgcgttg gttagataat
     1081 ggctatgcta taaaagccgg tatcacagcc tatcacagca tgagtataga cacggctgat
     1141 gatttagcaa aagcaataaa atatgccgtt gattttcaaa attaagacta tagtttttac
     1201 ccgttgtacg gtttaaacca catagaagca tagcattttt aatcataata gttgttcact
     1261 gaaatttgta gtgtaagtat tgttatctta aataaactat acatctatgt ggtgaaaatt
     1321 ataaatcaaa ctgcacacga gattagagtt ttattttcaa atagggtttt aattcagtaa
     1381 acgggatttc aaagctttgt cttccggcgg cataaggtgc aatatcatag tgattataca
     1441 aaaacaaaat gcccttttca gtaatggcaa aattttgaga cagcttaaat ttgccgtaat
     1501 ccggactgtc ctcgtcttca atattttcgc ctaatacaaa ataacctgca tctgctaaac
     1561 ttacagaatc gctgatttgc aattcctttc tgaaatattt ttctgccaat ttccataaat
     1621 tctcgtcttc agttttcagt atatcagata aaactacttt ttttaattta cttaaatcat
     1681 acacataata atcctggctg ccaaaagggt gcgcaccgcc taaaaatata tctgacgtat
     1741 aaaccaatgt atagtattgt ttgtttttgt aggcaggagt gatatcaata gttgaataat
     1801 actcccccgg caaatcatcg tattgatgta tggatagttt atcctgatga tagagttgaa
     1861 aaaaagattg aatataagct gaaacagatg tcgtagccga atcaaaatgc gataacaata
     1921 tttgttttgt ttcgttgttt aaatattgat ttatactgtc gtcctttgtt ttaaaatcgg
     1981 gatacccaaa atgtaagtta ggacaatttt cattcttcgc atctttacag gtaatgtctg
     2041 tcgtgtcaat tttaattgta tctaaaagca atgtatccgt tgcaatcatt ttttcgcgag
     2101 gcgattgaca acgaataaaa aagatgcccg ctaaaagact taataagagt agttttttca
     2161 taaatatgaa ttcgatacaa aagtagtatc ttattccaaa aaaactaaaa aaaagaattt
     2221 aattgagtgc cactacagac gtatttgttt gaacagtcgc agctgctttt tgctctcttt
     2281 tgcgtttgcg tcgttcattg tctagttttt cgcgttctgt gcctttataa atcagtcgcg
     2341 ttcccattac ataatcccat agtggaaatg ttacacacca gtttttatcc tgatcgggtg
     2401 ccatgtgatg atcataatgc caaggcagat atttatatcc ccattccgga tccaaatgag
     2461 attttttatg tactctgtaa taattgtatt gcgagagcca aagtgcactt acaaatccgg
     2521 gagctagcgg aaaaagcggt gctactatag ctgctgctga agccagcgac aatacttcgt
     2581 gtgattgcgg atttaactca tcaaagatgc gtttttcata agccggatct cggtattctt
     2641 gttgcagtac tgttttgtgg tggtcgtgcc agtgatagcg ccacatactg tgttttttct
     2701 tgccctggtc gtgtaagata tacttgtgaa aatgccattc aaaagcatta gcggtaatca
     2761 atcctgcaat aaatccgatc atagttgaat tttattttgt ttacctctaa tgtcgtaaat
     2821 aagccttaaa atttgcttta caaaagataa tttcaaagat tgctttatct ttagctcctc
     2881 gtgatgaaat ttagccttac accggattat acgatgactg tttttagctg tattgctcat
     2941 atacaaaagg acgactggca aagtataaag aaggatatgc aattctatca gtcttataat
     3001 tttttgcaaa ttattgaagc gttgcatccg gaacttcgta tgcgttatgc atttatttat
     3061 aagcaggaaa aacttttcgg aattgtttac acacaacaaa tggaattttc ctaccggcaa
     3121 gtttttactt actccgaatc aaaagaaaat atttttatca gaactattaa aaaatatctt
     3181 tccggcaaac aagtttattt gctcaatctc ggtgatgttt tttttaccgg agataaaggt
     3241 attctatctg aaaatgaaag tgatttattg cctctcatgc ccgaaatatt aaatcaaatt
     3301 aataatacat ttactgaaaa tagaagcggt gcatttttaa tggcaaacat tacactctca
     3361 gatgagtcga aatgtgtatt ttataaaagt caaaaatatc atccatttat tactgagccg
     3421 gatatgtaca tgcatatccg cgatgaatgg cattcttttc aggattatct gaatgcactt
     3481 agtgcaaaat acagaacacg agctaagaaa gtgatgagca cggcatcacc cattatatgc
     3541 cgagctctta ttgatgaaga agccttgctg tataaaaatg acatggctca actctatgcc
     3601 aatgtgatgg ataaaacaag ctttaatctc gccagattaa atcccgattt tttcatcagg
     3661 gtacgtcaag aatatccgaa taattgtaca ttgaaaggct attttctgga aggtaaaatg
     3721 gtcgcctttg tgtgcttgtt tgcgtgtaat gataaacaac ttcacatcca ttatatcgga
     3781 ttaaattatg aggtaaatgc tcaatataaa ctatataacc gtatgctttt tgactttttg
     3841 caatatgcca tagaaaacgg atttgataaa attcattttg gtagaacagc aacagaaatc
     3901 aaatcaactg ttggtgctca agctctccaa ttacatgctt atctaaaaat gaaaaacaga
     3961 ctcatgaatc tgatattgcc ggacatgctt gaaaaaatta aacctcaacc ttatacttta
     4021 agaaatccgt ttaaatgaca tataatagac atgaataatg ttttatatat gacgaaaatc
     4081 ttcctgttgc ttgtgtaaaa tcatttagaa agtgtaatta ttgattgaac tttacactag
     4141 aatttgaagt atgaaaaatt tattgatttt gggagcaggc acctccggaa caatgatggt
     4201 caatcatttg aataaggaat tatatgaaga tgactggaaa ctaaccattg tagatgaacg
     4261 cgaaaaacat tactatcagc ccggttatct ttttcttcct tttgatattt atgaacctga
     4321 agatatcata aaaccaatac aagaatttat tccggataga gcagaattga ttcgtaaaaa
     4381 aattgataaa gtagtacctg aaaataattt tgttttattg gcagatggca ctaaactgtc
     4441 ctacgatatt ttgattattg caacaggaaa tgatattgct cctgaagaaa ttgaagggat
     4501 gaaaggtgca gaatggcaaa aatcggtttt tgatttttat acattcgaag gagctttggc
     4561 attacgcgat aaattgcgca cttgggaagg cggaaaatta gtggtgcata taacagaaat
     4621 gccgattaaa tgcccggtag ctccattgga gttttcattt ttagcagatg cctattttaa
     4681 agaaaaaaac ctgcgcgata aagtggaaat cacttatgta acaccactaa gcggagcatt
     4741 tactaaacct aaagccaccg ctactctgga gcatttactc gtagaaaaag aaattaaaat
     4801 tgaaccggat ttcgccatcg aacacgtgga taatgaaaat aaaaaaattg tagattacgg
     4861 tggcaaagaa atttcatttg acttgctggt aactgtaccc accaataaag gcagtgaaat
     4921 ggtagcacgt tccggtttag gggatgagtt gaactatatt tctacttata aatcaacatt
     4981 gcaatcaaaa aaacatgcaa atatttttgt aattggtgat gctactgatg tacccgcttc
     5041 caaagccggt tcggtagcac attttgaagc agaaattctg acagagaata ttttgcgttt
     5101 tataaaaggc gaacctttaa aagaagaatt tgacggtcat gccaactgtt ttgtagaaac
     5161 aggaggaggc aaagctttgc tcattgattt taattataca cacgaaccgg tagaaggtga
     5221 gtttcctttt ccgggtgttg gtccgttgcg tttattaaaa gaaagccgta tgaaccacat
     5281 ggcaaagtta gccttccgct ggatatactg gcatatgttg ctgaaaggta ctcatattcc
     5341 atttattcct acacaaatga gcgaaaaagg caaacattat taatcaacta aaatttatat
     5401 aaaaatggaa a
//
LOCUS       DAOQII010000175         5395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8801_length_5395_cov_4.621161, whole genome shotgun sequence.
ACCESSION   DAOQII010000175 DAOQII010000000
VERSION     DAOQII010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8801_length_5395_cov_4.621161"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..646
                     /locus_tag="PLF48_08595"
     CDS             356..646
                     /locus_tag="PLF48_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49423.1"
                     /translation="MKKLILICVVAILGFSACSKEETILPAKTTSTSDAEKLMNENLT
                     SLEMQKPVSHLPLLEGEEKFVRCGSEIPSIKNPANLPQPGPPIVIIPEGEIR"
     gene            complement(772..1281)
                     /locus_tag="PLF48_08600"
     CDS             complement(772..1281)
                     /locus_tag="PLF48_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF49424.1"
                     /translation="MKFKEGKEHFIQTWGALGSQWGINKTIAQIHALLLVSNEPLSTE
                     DIMEQLAISRGNTNMNIRELIDIGLVDKVFKLGERRDYFVAEKDVWKVARQVAKTRKK
                     RELDPIIRLLEQIENLDVAKDDEEGKRFIKLVHDVKKLALNVEKLVDVLIKSEENWFW
                     DKIFKLMSK"
     gene            1901..5380
                     /gene="dnaE"
                     /locus_tag="PLF48_08605"
     CDS             1901..5380
                     /gene="dnaE"
                     /locus_tag="PLF48_08605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNF49425.1"
                     /translation="MCDFAPLHCHTQYSLLDGAADIDAMVKKAKEDNIPAVAITDHGN
                     MFGVFKFYSACMKHGVKPILGCEVYVVEDRFNKKFTRENRDKRYHQLLMAKNTVGYQN
                     LMKIVSLGYIDGLYMDFPRVDFELIKQYKEGIIATSCCIGAEVPQTIIHKGVEEAEKV
                     LKKWLDVFEDDYYIELQRHSIPDIDETGWSQEAVNQELIKLADKYELRVIATNDSHYV
                     NQKDSEAHDILLCLQTGKDFTDPDRFKFYNDQFFFKTQHQMKELFKDVPQAIENTLHL
                     AEKVEMLKLERDVLLPAFQVPFGFSNQNDYLRFLTFDGAKKRWGEILPIDIIERLDFE
                     LATIERMGFPGYFLIVQDFIREGRQMGVSIGPGRGSAAGSAVAYAIGITNVDPIKYNL
                     LFERFLNPGRVSMPDMDIDFDDEGRERVINYVIDKYGKQQVAQIITYGTMAARSSIKD
                     VGRVLKLPLSETDKMSKMVPEGPKVTLASAFKEVEELRKIRDQKGDLRARTLELAEVL
                     EGSVRNRGIHAAGVIIAPENIMNYVPVCTAKDADLLVTQFDGKYIESAGMLKMDFLGL
                     KTLTIIKDALSLIEKNHKIKIDIDNIPLDDKKTYELFQKGDTIGIFQFESAGMQKYMK
                     ELKPDCFEDLIAMNALYRPGPMGYIPNYIARKHGKEPVTYDLQEQDTILKDTYGITVY
                     QEQVMLLSQHLAGFSKADADNLRKAMGKKDRAKLDSLKESFIEGCKKNNLEIEKCFKI
                     WVDWEKFAEYAFNKSHSTCYAYLAFQTAYLKSHFPAEFMASVLSHNMNDIKQVNFFLS
                     ECRRIGVPALGPDINESDVKFSVNKPGEIRFALSAVKGCGEAAVCDIIEERNKNGAFA
                     SIFDLTKRMNSRTVNKKILEALAQSGAFDCFDNIHRAQYFYQYKDDTTFIERALRWGN
                     NVKEREADNSMSLFGNTSDAVIPEPEVPHTNPWATFEKLQKEKDVTGIYISGHPLEEY
                     QLVTNHYANCSFDSLEQAKDKDIAITCMVAGVNRRVDKNGRPWAIVQFEDLESMQECF
                     FFSEDYNKFLPFLEQGNRLLIKGIYRPSYRDKTQYELKIQSIEFIQDVLEKYCRGIRV
                     ILNYKELDEAKVNAIYKVVQKHSGKRVFEVYLVDDNDQPLSFRSEKSGIKVSKDLFDS
                     LSEIEGLRVRLII"
BASE COUNT         1744 a          947 c         1098 g         1606 t
ORIGIN      
        1 aaaaatctgg acatctgccc ggcagtaaaa cgaatttttg atgctttatt gtctgctaat
       61 ttttgaatga aaaaaatcac attttagcat tccctttact tatcattctc gaagtagtat
      121 attttgattt ccttgtgatt tcaaaaagaa agatgctttt taaaaaatgc atccaagatg
      181 cttagcaagc gactaagcca ataaacatta taactgtcat ttgtttttca gtaggtttca
      241 aaatctgaca tgtttctaaa attaccactt acacaaaaat aaataccatt cgctaattaa
      301 aaatacacgg tttgaatgga aggaaataca tttgaattac aaaaacaata aaactatgaa
      361 aaaattaatt ttaatctgcg ttgtagccat acttggtttc agcgcctgca gcaaagaaga
      421 aaccatcttg cctgccaaaa caacatcaac ctctgatgca gaaaaattga tgaatgagaa
      481 cttgacaagc ctggaaatgc agaaacctgt aagtcatctt cctcttcttg aaggtgaaga
      541 aaaatttgtt cgttgcggta gtgagattcc gagcatcaaa aatccagcaa atttgccaca
      601 gccgggtcca ccaattgtta taatacctga aggagaaata cgataaagaa agaatatgcc
      661 tttgaggcag actatgaaaa caatgatgcg acgtcctctc tgaacaacaa actagttgga
      721 attggagagg gcgttatttt gcttagaaat tatcagcagg aaatttttat cttatttaga
      781 catcagtttg aaaattttat cccaaaacca gttctcttca cttttaatca gtacgtctac
      841 taatttttcc acattaagcg ctagtttttt tacatcgtga accagcttaa taaagcgttt
      901 gccttcttca tcatctttcg ctacatccaa attttcgatt tgttccaaca accggatgat
      961 aggatctagt tctctttttt ttcgggtttt ggcaacttgt ctggccactt tccatacatc
     1021 cttttctgcc acgaaataat ctcgtcgttc gcccagcttg aatactttgt ccactaatcc
     1081 gatatcgata agctcccgga tattcatatt cgtattgcct ctcgaaatgg ctaactgctc
     1141 catgatatct tctgtgctga gaggttcatt gcttactaaa agcaaagcgt gaatttgcgc
     1201 tatagtttta ttgatacccc attggctgcc taaggcaccc caggtttgaa tgaaatgttc
     1261 ttttccttcc ttgaatttca tatacaaaat tagcgtttac ttttcattat ttcaataaat
     1321 tctgaaacca tttttaaaga taaattaccg aatactcaat gaaacatacc gaatactcat
     1381 ttctttatgc actattgaca aagaaattta cttttaccat gcaagatcac agaaaccaat
     1441 aatacagaag aatccaagaa gacctttcta ttaatattta gttgcgtgtt tagagtcccg
     1501 ggtgcaaggc ccgggcattt ttttatagct ttttgatttt tccaaaaact taaacaaatc
     1561 tggtatcgaa gttttttgac cacttaatca ctcagattgc cgtttaatca atggatttct
     1621 ttatcccgtt tatttttagg aatattcgtg cataaccgtg aggttatatt ccaacttaag
     1681 tttagtttag caaaaaaagg ctgctctcca cgtgaggcag ctttttactt ttttgggcat
     1741 tctatgtttt taaaagaaga agatgatccg ttacataaca atccgtaatt tcagactgaa
     1801 attgaatttt gttgaaaaat taaatggtag atttatccgg ttatttcccg catcatcgta
     1861 tttttataca gattttccac ctttttaaat tcattttctg atgtgtgatt ttgcccctct
     1921 ccattgtcat acccaatatt cattattaga tggcgctgcg gatattgatg ccatggtaaa
     1981 aaaggcaaaa gaagacaata tcccggctgt agctattact gaccatggga atatgttcgg
     2041 tgtatttaag ttttacagtg cctgcatgaa gcatggcgtg aaaccgatat taggttgtga
     2101 agtctatgta gtggaagatc gttttaataa aaagtttacg cgagaaaata gagataaaag
     2161 ataccaccag ctcctgatgg caaaaaatac tgttggttat caaaatctga tgaagatagt
     2221 ttcattaggt tatattgatg gtctatatat ggattttccc cgtgtggatt tcgaacttat
     2281 caaacaatat aaagaaggta taattgctac atcctgctgt attggagcgg aagtaccaca
     2341 aaccatcata cacaaaggag tggaagaagc agaaaaagtg ctgaagaaat ggctggatgt
     2401 tttcgaagat gattattata tcgaattgca acggcacagt attccggata ttgatgaaac
     2461 aggatggagt caggaagccg ttaatcagga gctgataaag ctggccgata aatatgagtt
     2521 aagagtaatt gcgacaaacg attcgcatta tgtcaatcaa aaagacagcg aagcgcatga
     2581 tattttattg tgtttacaaa caggcaaaga ttttaccgat cccgaccgtt tcaagtttta
     2641 taacgaccag ttctttttta aaacacaaca ccaaatgaag gaactcttta aagatgttcc
     2701 gcaagcaatt gaaaacacac tgcatctggc agaaaaagtg gagatgctga aattggaaag
     2761 agatgtttta ttgccggcat ttcaagtgcc ttttggtttc tccaatcaaa atgactacct
     2821 gcgtttccta acttttgatg gtgcaaaaaa acgctgggga gagatattac ctattgacat
     2881 cattgagcgg ctggattttg aactggccac catcgaacgc atgggtttcc cgggatattt
     2941 tctgattgta caagatttta tcagggaggg cagacaaatg ggcgtatcca ttggtccggg
     3001 cagaggctct gcagccggct cagcggttgc ttatgccatt ggtattacca atgttgaccc
     3061 tataaaatat aatctgctgt ttgaacgctt ccttaatccc ggtagggtga gcatgcccga
     3121 tatggatatc gattttgatg atgagggcag ggaacgtgtc atcaattatg tgattgataa
     3181 atatggcaaa caacaagtag cccagattat tacctatggc acaatggcgg cacgaagctc
     3241 gataaaagat gtgggtcgcg tattgaaact gccactttca gagacagata aaatgtcgaa
     3301 aatggtaccc gaagggccaa aagttacctt ggcatccgct tttaaagaag tagaagaatt
     3361 gcgcaaaata agagatcaaa aaggggattt gagagcacgg acgttagagc ttgctgaagt
     3421 tcttgaaggc tccgtgcgca acagaggcat ccatgctgcc ggagtaatca tcgcacctga
     3481 aaatatcatg aactatgtgc cggtgtgtac tgccaaagat gcggatttat tggttaccca
     3541 attcgacggg aaatatatag aaagtgctgg catgctgaag atggattttt taggattgaa
     3601 aacacttacc attattaagg atgccctttc actcatcgaa aaaaaccata aaataaaaat
     3661 agatatcgac aacatcccgc tggatgataa gaagacgtat gagctgtttc aaaaaggaga
     3721 taccattggt atatttcagt ttgaaagtgc gggtatgcaa aagtatatga aagagctgaa
     3781 acccgactgt tttgaagatt taattgcgat gaatgccctt tacagaccgg gtccaatggg
     3841 gtatatcccg aattatattg ccagaaaaca tggcaaagaa ccggttactt atgatttgca
     3901 ggagcaagat accattttga aagatactta tggcatcacg gtataccaag agcaggtcat
     3961 gctactttca caacatcttg cagggttttc taaagcagat gccgataatt tgcgcaaggc
     4021 catgggaaag aaagaccggg ctaaactgga ctcactgaaa gagagcttta ttgaaggatg
     4081 caagaaaaat aatttagaaa tagagaagtg tttcaaaatt tgggtggatt gggagaaatt
     4141 cgcagagtat gcattcaata aatcacattc tacttgctat gcctatctgg catttcaaac
     4201 agcctattta aaatcgcatt ttcctgctga atttatggca tcggtattgt cgcacaatat
     4261 gaatgatatc aaacaagtta atttcttttt gagtgaatgc cgaagaatag gggtaccggc
     4321 attaggaccg gatatcaacg aaagcgatgt gaagttttct gtgaacaaac caggagagat
     4381 tcgatttgca ctaagtgcgg taaaaggttg cggagaagct gcagtttgtg atatcattga
     4441 agaacgtaat aaaaatggtg catttgcttc tatttttgat ttgactaaaa gaatgaattc
     4501 acgtacagtg aataaaaaaa tactggaagc actggcacaa tcgggagcat tcgattgttt
     4561 tgacaatata caccgggcac aatattttta ccaatataaa gacgacacta cctttattga
     4621 aagagccttg cgttggggaa ataatgtaaa agagcgtgaa gctgataatt cgatgtcgct
     4681 gtttggcaat acgtccgatg cagtcattcc tgaacccgaa gtgccgcata ctaatccgtg
     4741 ggctactttt gaaaaattgc aaaaagaaaa agatgtaacc ggtatttata tttccgggca
     4801 tccgttggaa gaataccagt tggttactaa tcattatgcc aattgttctt ttgatagttt
     4861 ggaacaagca aaagataaag acattgcaat tacgtgtatg gtggcaggcg ttaatcggcg
     4921 agtggataaa aacggcagac catgggctat cgtgcagttt gaagacttgg aaagcatgca
     4981 ggaatgtttt tttttcagtg aagattacaa caaattttta ccgtttttag agcaaggcaa
     5041 ccgattgctg ataaaaggta tctatcgtcc cagttacaga gataaaacac aatatgaact
     5101 gaagatacaa agcatagaat ttattcagga tgtactggaa aagtattgca gaggcattcg
     5161 ggttatttta aactacaaag agttggatga ggcaaaagta aatgccatct ataaggttgt
     5221 acaaaagcat agtggtaaaa gagttttcga agtgtatctg gttgatgata atgatcagcc
     5281 cttatcattt cgttcagaaa agtcgggcat aaaagtcagc aaagacctgt ttgattcact
     5341 cagcgaaatt gaaggattgc gggtaaggtt gattatttaa acaaatctta cttgt
//
LOCUS       DAOQII010000176         5357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8890_length_5357_cov_4.277254, whole genome shotgun sequence.
ACCESSION   DAOQII010000176 DAOQII010000000
VERSION     DAOQII010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8890_length_5357_cov_4.277254"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLF48_08610"
     CDS             <1..400
                     /locus_tag="PLF48_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49426.1"
                     /translation="GDKKLFYINRIIIVCLALISIYFSIQQIQHPKLSVSLFALVGVY
                     GYFATAFVPILFGIFIKYTIPIYIPFTASIVALVTHFGMYYGKMTKYLQGPIGNPAVS
                     CATALVLSFLTGLLLLQLNKSKNQTKLPVS"
     gene            397..1407
                     /locus_tag="PLF48_08615"
     CDS             397..1407
                     /locus_tag="PLF48_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNF49427.1"
                     /translation="MKKWIKNKIYLLTIFLFIACIPSGSTTEVFDTNYSVSKLSSDNS
                     ANTSAVSITDDFKNWLNANGYGSYHFEYNGTYGNSYGGKQTTSTAVINQPVIFIHGNG
                     DIAVGSTSLQAGWTNSINYFIQKGYTSAELYAVSWGSGNIAQTALEYHSKTNVMRIRK
                     FIEAVIAYTGATKVDIITHSMGVTLTRKAIKGGTANDLLAGGSYQVGTALTSKVDAFV
                     GIAGANYGLTSCYASGPTTPTCGATNGFYPGTAPGGIGLSAYLADLNASSGYEGAFRA
                     TMWSSVDEIIGLGCLVWGQITCRIPGQNTEKKYTAAPYGHYGLKDQTCPVQYNLVKNH
                     TL"
     gene            1409..2899
                     /gene="menE"
                     /locus_tag="PLF48_08620"
     CDS             1409..2899
                     /gene="menE"
                     /locus_tag="PLF48_08620"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HNF49428.1"
                     /translation="MFDWVGKWALTTPNKTALKEAETGRTLSYSEWNNQANTVVHYFS
                     SIGLKKGDRIAILAENCLEYFILFSVCQKMGTILVPLNYRLTSAEINYLLNNASPDLL
                     IVEDKFSQLATATEYYSKLKHGISLSAFTAYCTQSETDLSKVQYPEAVSMDDSLFIIY
                     TSGTTGHPKGALYTHKMLFWNSINTTLRMDNTSESRSLVCMPLFHTGGWNVIGTPFIH
                     RGAFICVQKTFDPALTLQLMQDEKISIFMAVPTMLKILADQPDFENADLSSVEYFMVG
                     GESLPIPLIELWDKKGIPIRQGFGMTEAGPSLTSLHQTDCIRKIGSIGTPNFYVQIKI
                     VDENGMEVGVNETGEMLVKGDMVLPGYWNNPEASKEAFTDGWFHTGDILMKDEEGFLY
                     VKDRKKNMFISGGENVYPAEIEKYIYTHPQVAEVAVVAMKDERWGEVGHAYVVLKENI
                     QLTADEIIEYCKKGLAKFKIPKAITFLNELPKNAAGKTDKKLLTCN"
     gene            2938..3480
                     /locus_tag="PLF48_08625"
     CDS             2938..3480
                     /locus_tag="PLF48_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49429.1"
                     /translation="MNTISKKLFATLLLFVAITALVSSCKKDDDTTTTKTPEQVIAGT
                     WLSQGADVAPILLGAPLKTKKITATFNSNSTYSVVATDSSNANTTYEGTFDADTTNVS
                     GIYKITTTTSKVNGVAYVIESKGIFRIDEAATPDYMTYEVAQTTPVIAGVTPPTAQAG
                     FGSTSGSAFGTLNVQHFKKQ"
     gene            3552..5126
                     /locus_tag="PLF48_08630"
     CDS             3552..5126
                     /locus_tag="PLF48_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49430.1"
                     /translation="MRLKYLIFGLILQLSFTSFAQDMWTGRKTDFPVKEAKDLKFYGY
                     FINQFVVNNIYATNDFLKGQTVGRLYGGNTTTSSDTNRSIYAEQRFIPFFEYLPSLLN
                     RKVAIRAAFEIDWTWGDVAYGAGGNLGGGFSADQVNIQTQLAELEFSPKPNIQINLGM
                     TRLFDTPKNPFAVSFNNMTKTGTRMHLFGSEAVGTSINYAGDDWCTKAGFYWLYSNNI
                     KDRDDVTVFEAQGQKDFKKNWSLGGSFYYLRDRSHSLSKNNINGGVSILGQGLRGSLA
                     DYNGTYNFNVVGSEVKGDFFWLSTFWGKNEEYNTGRFMHTGYITANLGIVDTNRAKWT
                     KDFSIAGVAANAKIGYRYGQTYEDRIELDAFFSTGDNNTNDGRYSGVITGNQWGSPGA
                     LFLGTEALLLFPQPNVVNRYFAAVTDISNQGLGLIGTSLHAHKDIIPNKLSAKIGTAT
                     ALSVISPKNGTNYMGTEISAAISWQPKILMNLELHGGYLMLGNFYKDFKMNGIADGVK
                     PTNPWTLFLVYKWLIF"
     gene            5145..>5357
                     /locus_tag="PLF48_08635"
     CDS             5145..>5357
                     /locus_tag="PLF48_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49431.1"
                     /translation="MKSTFFIFILISPFFLFAKKEKNKPTAKEKMEFSELSYPFETKF
                     QQLDSFNINLAYVDEGKGDKTIVFIHG"
BASE COUNT         1742 a          938 c         1080 g         1597 t
ORIGIN      
        1 tggggataaa aaattgtttt atattaatag aataatcatt gtctgcttgg cacttatttc
       61 tatatatttc tccattcagc aaatccagca tcctaaatta agcgttagct tatttgcatt
      121 ggtaggagta tatggatatt tcgcaactgc atttgtcccc attttatttg gaatttttat
      181 taaatacacc attcccatct atatcccctt tacagcttca atcgtagcat tagttacaca
      241 ctttggaatg tattacggaa aaatgaccaa atatttgcaa gggcccatcg ggaatcctgc
      301 tgtatcttgt gcaacagcct tagtgctttc ttttttaact ggcttactct tattacaatt
      361 gaataaaagc aaaaatcaaa ctaaattgcc cgtgtcatga aaaaatggat taaaaataaa
      421 atatatttgc tgacaatctt tctttttatt gcctgcattc cttcaggaag tacaacagaa
      481 gtgtttgata cgaattattc tgtttccaaa ttatcttcag ataattctgc caatacaagt
      541 gcagtaagca ttactgatga ttttaaaaac tggctgaatg caaatggtta cggtagttat
      601 cattttgaat acaatggtac ttatggaaat tcttatggcg gaaaacaaac aacatccact
      661 gccgtcatca accaaccggt tatatttatt cacggcaatg gagatatagc tgttggaagc
      721 actagtttgc aagcaggctg gacgaattct ataaactatt tcatacaaaa aggatacaca
      781 tccgcagagt tatatgcagt ttcctggggt tcgggtaata ttgctcagac tgcattggaa
      841 taccattcga aaacgaatgt gatgcgcatc cgtaaattta tagaagcagt aatagcctat
      901 accggagcaa caaaagtaga tatcatcacc cattctatgg gagtaacctt aactcgtaaa
      961 gcaattaaag gaggcacagc aaatgattta ttagcaggag gaagttatca ggtaggcacg
     1021 gctcttactt ctaaagtaga tgcctttgtc ggtattgcag gagcaaatta cggacttaca
     1081 tcatgctatg cctctggacc aactacccct acttgcggtg caactaatgg gttttatccg
     1141 ggtacggcac ccggtggaat cgggctttct gcttatttgg ctgatttgaa tgcaagctct
     1201 ggctatgaag gtgcattccg tgcaactatg tggtcgtcgg tggatgaaat tatcggatta
     1261 ggatgtttag tctggggaca aattacttgt cgtattccgg gacaaaatac tgagaaaaaa
     1321 tatactgcag caccttacgg acattatgga ctgaaagacc aaacttgtcc ggtacaatat
     1381 aatttggtaa aaaatcatac gttataagat gtttgactgg gttggaaaat gggcattaac
     1441 tactccgaat aaaacggcac tgaaagaagc cgaaacgggc agaactttgt cctactcaga
     1501 atggaataat caagcgaata ctgtagttca ttatttttcc agtattggtt taaaaaaagg
     1561 tgaccgcatt gccattttag cagaaaattg cttggagtat tttatcttgt ttagtgtgtg
     1621 tcaaaaaatg ggaactattt tagtcccatt aaattacaga cttacttctg ccgaaatcaa
     1681 ttatttattg aataatgcat ctcccgattt gttgatagta gaagataaat tcagtcagct
     1741 tgctactgca accgaatatt acagcaaatt aaaacatggc atttccttgt ctgcttttac
     1801 ggcatattgt actcaatcag aaacggattt atcaaaggtg caatatccgg aggcagtgtc
     1861 catggatgat tcactattta taatttatac cagtggaaca acaggccatc ccaaaggggc
     1921 tttatacaca cacaaaatgt tgttttggaa cagtatcaac accacgctcc gaatggataa
     1981 cacatcagaa agccgatcac tggtttgcat gccactgttt cataccggcg gatggaacgt
     2041 catcggaacg ccttttatac atagaggagc ttttatttgt gttcaaaaaa catttgatcc
     2101 ggctttgact ttgcaactta tgcaggatga gaagatttcc atttttatgg cagtgcctac
     2161 catgctgaaa atattagcag accaaccgga ttttgaaaat gcagatttat cgtctgtaga
     2221 atattttatg gtaggaggtg agtcgttgcc cattccgctt attgaattgt gggataaaaa
     2281 aggaattcct atccgacaag gatttggtat gacggaagca ggtcccagcc tgactagttt
     2341 gcatcaaact gattgcattc ggaaaattgg ttcaatcggt acccccaact tttatgtaca
     2401 aataaagatt gtggacgaga atggtatgga agttggtgtg aatgaaacag gagaaatgct
     2461 ggtaaaagga gatatggtac tgccgggcta ctggaataat cctgaagctt ctaaagaggc
     2521 attcacggat ggttggtttc ataccggcga cattttaatg aaagatgagg agggatttct
     2581 gtatgtaaaa gacagaaaga aaaatatgtt catttcggga ggcgaaaatg tttatcctgc
     2641 tgaaattgaa aaatatatct atacgcatcc acaagtagca gaagtggctg tagtggctat
     2701 gaaagatgaa cgttggggcg aagttggtca tgcatatgtg gtattaaagg aaaatataca
     2761 acttacagca gatgaaatta ttgaatattg taagaaagga cttgcaaaat ttaagattcc
     2821 taaagcaatt acattcttga atgaattgcc aaaaaatgca gcaggaaaaa cagataagaa
     2881 attactaaca tgtaattaat aaacaaattt attattaaca ctaaaaagat caaaaaaatg
     2941 aacacaatct caaaaaaatt attcgcaaca ttacttttat ttgttgctat tactgcactg
     3001 gttagcagct gcaagaaaga tgatgacacg acaacaacta aaactccgga acaagtaatt
     3061 gcaggaacat ggttgtctca aggtgctgac gttgcaccta tcttattagg agctccacta
     3121 aaaacgaaaa aaattacagc tacatttaat tccaacagta cctatagtgt tgtagctaca
     3181 gacagctcaa atgcaaatac tacttatgaa ggaacttttg acgcagatac gaccaatgtt
     3241 tcaggtattt acaaaattac aactacaaca agcaaagtaa acggtgtagc atacgtaatt
     3301 gaatcaaaag gtatattcag aatagatgaa gctgcgactc cggattatat gacctatgaa
     3361 gtagcacaaa cgactcctgt cattgcaggt gttactcctc caacagctca agcaggtttt
     3421 ggcagtacaa gtggcagcgc atttggcact ttgaatgttc agcattttaa aaaacaataa
     3481 tttattcaac aggaaacagg ctaacaattt agcctgtttc ccattttttt tgccccttaa
     3541 aatctttagt tatgcgtttg aaatatttaa tattcggact gatattacag ctgagtttca
     3601 cttcttttgc tcaggatatg tggacaggaa ggaaaacgga ttttccggtt aaagaagcaa
     3661 aagatttaaa attttacggc tattttatca accagtttgt tgttaataat atctatgcaa
     3721 cgaatgattt tctaaaaggg caaaccgtgg gaagattata tggtggcaat accactacat
     3781 cttccgatac gaatcgttct atctatgcag aacaacgatt tattccattt tttgaatacc
     3841 tgccttctct gttaaaccgg aaagttgcta tccgggcggc ttttgaaatt gactggacct
     3901 ggggcgatgt cgcctacggt gcaggtggta atttaggggg aggtttctcg gcagatcaag
     3961 tcaacattca aacccaattg gctgaattag aattttcacc caaaccgaat atccagataa
     4021 atctgggtat gacacgctta ttcgatacgc ccaaaaatcc ctttgccgta tcgtttaaca
     4081 atatgacaaa aacgggcaca cgtatgcatc tttttggctc agaagctgta ggcacgtcta
     4141 taaactatgc aggtgatgac tggtgtacta aagcaggttt ttactggctg tattccaata
     4201 atataaaaga ccgcgatgat gttacggttt ttgaagcaca aggacagaaa gattttaaaa
     4261 agaactggtc gttaggcggt tctttttatt atttgcgaga tcgttctcat tcgctttcaa
     4321 aaaataatat caatggcgga gtatccatcc tcggtcaggg gctacgagga agtttggcag
     4381 attataacgg aacttataac tttaatgtgg taggctcaga agtcaaagga gacttcttct
     4441 ggctgagtac tttttggggt aaaaatgaag agtataacac aggtagattt atgcataccg
     4501 gatatataac ggcaaactta ggaatagtag ataccaacag agctaaatgg accaaggatt
     4561 tttcgattgc aggtgttgca gcaaatgcaa aaataggata ccgctacggg caaacatatg
     4621 aagaccgcat tgagttggat gcattctttt caaccggtga taataacacc aatgacggca
     4681 gatacagtgg tgtcattact ggcaatcaat ggggctcacc gggggcgtta tttttaggta
     4741 ctgaggcttt gcttttgttt cctcagccta atgtagtcaa cagatatttc gcggcagtta
     4801 cagatatttc caatcaagga ttaggattaa tcggtacttc gctacacgca cacaaagata
     4861 ttattccaaa taaactttct gcaaaaattg gtacagcaac tgcattatcc gtgatatcac
     4921 cgaaaaacgg gacaaattac atgggtacag aaatcagtgc ggccatttct tggcagccaa
     4981 aaattttaat gaatttagaa ttgcatggcg gctatttgat gctggggaat ttttataaag
     5041 atttcaagat gaatggtatt gcagacggag taaagcccac aaatccatgg actttgtttt
     5101 tagtttataa atggttgatt ttttaatact tttaattcta tttgatgaaa agcacgttct
     5161 ttatttttat tttaatcagt cctttttttc tgtttgcaaa aaaagaaaaa aacaaaccga
     5221 cagctaaaga aaaaatggaa ttttctgaat taagttatcc attcgaaact aaattccagc
     5281 aactggattc attcaacata aatttagctt atgtggatga aggaaaagga gataaaacca
     5341 ttgtttttat acatggt
//
LOCUS       DAOQII010000177         5328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_8957_length_5328_cov_4.898919, whole genome shotgun sequence.
ACCESSION   DAOQII010000177 DAOQII010000000
VERSION     DAOQII010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8957_length_5328_cov_4.898919"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="PLF48_08640"
     CDS             complement(<1..1675)
                     /locus_tag="PLF48_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNF49432.1"
                     /translation="MEETTEKIYTDIHPQDEDANRQFEEYVEQKPSSLIVRLFVTTRH
                     LTGLALGGGYAYMRYRRKTKAKFSILQDVFLRLYLWLNWPFLKKKLIKQPFSVQLRRR
                     LEMLGATYIKLGQILSLREDMLPKDITDELKKLLDTLPAVKSDRFMELVEIQINQPIH
                     KVFSEVKQNPLGSASIAQTHRATLRSGEEVVLKLLKPGTRELIQTDSKIIKAMGSFLQ
                     LFLPHLQPKNMFTEFCDYTNREVDFRLEADNAEEFAANFAKHPAIHFPKIYREYTTSD
                     LICMEFIRGIKPDKRAYERYNEEEREKIVDYGAYAIIQMLYHDGFFHADLHPGNLLIV
                     DSPNGPSVGFIDLGMVGRFEENTRKIMLYYFNSLVMGDPGGAARYLTLLARPGKGADI
                     EGFRKEFVSVGNRWLKAPNFKEFSLARLILESVALGSKYRLYYPLELVLMVKAIITYE
                     GVGNVILPGFDIQKVTKKHIRRMVLGEVNPLDAMKAQLQNVPAGEHGFHIHEFGDCGN
                     VGANAGSHYNPQNTPHGLVSKDGVKKAHGGDMGNITAKADGTATLDILLP"
     gene            complement(1790..3496)
                     /locus_tag="PLF48_08645"
     CDS             complement(1790..3496)
                     /locus_tag="PLF48_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF49433.1"
                     /translation="MKKLFFLGLFFACVWNSINAQDPHFSQIQYNPLYLNPSYAGFGD
                     KDNRFVGMYRDQWRTVPVPFMTAWGSYDRCFICKPQKYFRFGGGLSFLFDRAGDGLLS
                     TFNPNITLAVSKGFAKGRFWTSFGATAGMTIKSLDYGRLTFDNQYIVGTGYVPGSATG
                     ETFSNNNVSFANFGLGLNFRYKFKKISYVDLGGTIWNPHQPKQNFLYFTESRLPMRYG
                     AYTKARIELGKKEMWNLQPGFFFNRQNKVNNMLLNAIAEMQFAKSNVGLGFGAGYRFE
                     SNDAAIAYLSVLYKGLRVGASYDINVSKFTKATRFNGAFELLLSYEFGEKACSKKCPE
                     LPKCPACPPYENTEVEIKVKQRDNDSLLATFKKSFEADTIPQTYLDSLYVSIPKVEKP
                     MISEIVLKMLPLTLYFHNDIPKTGDNTNYEDLYTAYVARVPEYKEKSIDDDARVDEFF
                     TKVETNYNNLNKVIEEINAVIKKGYTVDIEFRGYASPLAPASYNKALTERRISSIMNL
                     FKERMGANYDKISKTTLPLGKSVAKTGISDKLSEPSKSIFGIDASSERKVEIVGIRLY
                     KE"
     gene            complement(3521..>5328)
                     /locus_tag="PLF48_08650"
     CDS             complement(3521..>5328)
                     /locus_tag="PLF48_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF49434.1"
                     /translation="PTAGQCATTTTMSIVVNAIIRTSTDITICQGQSYTFNGQELTES
                     GTYFDTLTSAYGCDSFSTLNLTVTDALTLNIDNSICEGQSVTIGTQTFNASGDYTVVL
                     QTTGGCDSIVHLHLVVNSPTSSQLEKTICEGSSYTLGSQTFDQSGNYTVHFTNSAGCD
                     SSVYLNLTVNPVSRTEINTAICQGDTYTVGTQTFNQSGTYTVTLQNSVLCDSIITLNL
                     SVNPTYLIEDTRTICEGDSLIYNGIKYMEAGTYPQHFYTSAQCDSIVNLVLQVTPKSR
                     STVNTTICSGQSYTIGTQTFNQSGTYFITLTAASSCDSIVTLNLTVNPPLTSTINKSI
                     CAGDSYTIGSQTFNASGTYTVTVQNQSGCDSIITLNLSVSEPLVSNINRIICRGEEVV
                     IGDSTFTNAGMHHAILQSSAGCDSTVNLDLTVIDGPDVTISTSLTNPVAGQEFQLHAS
                     SPLATTFNWTPVSNVTSPTDSITAATIIETTWFYVTVTGSNTRCATTDSILIELKEIE
                     CTFDNLYIPNAFTPNGDEKNDLFIVRGPLLKSMSMLIYDRWGTVVFETNDLGTGWDGT
                     YKGKLVQADVFGFIIRGECSNGTIITKKGNVTVLR"
BASE COUNT         1621 a         1051 c          925 g         1731 t
ORIGIN      
        1 cgggaagcag aatatcgagg gtggccgttc catcggcttt cgcggtgatg ttgcccatgt
       61 caccaccatg cgcttttttc acaccgtctt tggaaacaag gccatgaggc gtgttttgcg
      121 ggttgtagtg ggagcccgca ttggctccga catttccgca gtccccgaat tcatggatgt
      181 gaaaaccatg ctcgccggcc ggaacatttt gcaattgtgc tttcatggca tccaaaggat
      241 ttacttcgcc cagcaccatt cttctgatat gttttttggt tactttttga atatcgaatc
      301 cgggtaatat gacattgcca actccctcat aagtaatgat ggcttttacc attaaaacca
      361 attccaaggg ataatacaaa cgatatttag aacctaatgc aacagattcc agtatcagcc
      421 gtgcgagtga gaattctttg aaattaggtg ctttcagcca tctgtttcct acggaaacaa
      481 attcttttct gaaaccttca atatctgctc ctttacccgg gcgtgccagt aatgtaaggt
      541 atctggctgc tccacccgga tcacccatca ctaaactatt aaaataatag agcataattt
      601 ttctggtatt ttcttcaaaa cggccaacca tacccaagtc aataaagcct acactgggac
      661 catttggaga atctacaatc agcaagttgc cagggtgtaa gtcggcatga aagaacccat
      721 cgtgatacag catttgaata atggcataag ctccgtaatc aacaattttt tctctttcct
      781 cctcattata tctttcgtaa gcccttttat ccggttttat tccccgaata aattccatac
      841 atatcaaatc actggttgta tattctctgt atattttagg gaaatgaata gcaggatgtt
      901 tggcaaaatt tgctgcaaat tcttctgcat tatctgcttc caagcgaaaa tcaacttctc
      961 tgtttgtata atcacaaaac tcagtaaaca tatttttggg ctgcaaatgc ggaagaaaaa
     1021 gctgcaaaaa tgaacccatt gcttttataa ttttgctgtc cgtctgaatc aattctctcg
     1081 ttcccggttt tagtaatttt aatactactt cttctccgga acgcaaagtt gctctgtggg
     1141 tttgtgcaat tgaggctgaa cctaaaggat tctgttttac ttcagaaaat actttatgta
     1201 taggttgatt aatctgaatt tctaccagtt ccataaaacg gtcagatttc acagcgggta
     1261 aggtatctaa tagtttttta agttcatcag tgatgtcttt tggtaacata tcttcacgta
     1321 aactcagtat ttgtcctagt ttaatatagg tggcacccaa catttccagt cttctgcgaa
     1381 gttgaacaga aaagggttgt ttaatcagtt ttttctttaa aaatggccag ttaagccaca
     1441 aatacaagcg caaaaacaca tcctgaagaa tgctgaattt tgctttagtt ttacgacgat
     1501 agcgcatata agcataacca ccgccaagtg ccaaaccggt aagatggcgt gtagtcacaa
     1561 acaagcgaac aattaaagaa ctgggttttt gttcaacata ttcttcaaac tgtctgtttg
     1621 catcttcatc ttgtggatgt atgtcagtat atatcttttc ggttgtttct tccatgctat
     1681 cgggataatc gtctaagata gtaaaaacag ttaacttttc atataactga ctatataaaa
     1741 aaaaagcagc atatcagata tgctgctttt tagtatctaa gatattttat tattctttat
     1801 acaatctgat acctacaatt tccactttac gttcagaaga tgcatcaata ccaaaaatag
     1861 atttagaagg ctctgaaagt ttatcactaa ttccggtttt tgcaactgat ttacccaacg
     1921 gcaatgtagt tttggaaatc ttgtcataat tagcccccat tctttcttta aataaattca
     1981 tgatagaact gatacgtctt tctgttagtg ccttattgta agatgccgga gctaatggag
     2041 aagcatatcc tctaaattca atatctacag tatatccttt tttaataacg gcatttattt
     2101 cttcaattac tttattcaga ttattataat tcgtttctac tttggtaaag aattcatcta
     2161 ctcgagcatc atcatcaata gatttttctt tatattccgg tacgcgagct acataggctg
     2221 tataaaggtc ttcataattc gtattgtcac ccgttttagg aatatcattg tggaaataca
     2281 aggttagtgg taacattttc aatacgattt cagaaatcat aggtttttca actttaggga
     2341 tacttacgta taaggagtct aagtaagtct gaggtatagt atccgcttca aaagatttct
     2401 tgaatgttgc caataagctg tcattatcac gttgcttcac tttgatttcc acttctgtat
     2461 tttcataagg cggacaagca ggacatttag ggagttcagg acattttttg ctgcatgctt
     2521 tttcaccaaa ttcgtaagaa agcaataatt caaaagctcc gttaaatctg gttgcttttg
     2581 taaatttcga cacattaata tcgtagcttg cacccactct caatccttta tacaatactg
     2641 ataaataagc tattgcagca tcgttgcttt caaagcgata tcctgcacca aagcctaagc
     2701 ctacattaga ttttgcaaac tgcatttcag caatggcatt caataacata ttatttacct
     2761 tgttttgtcg gttaaagaaa aaacccggct gcaaattcca catttctttt ttacccaatt
     2821 caatacgggc cttagtatat gcaccataac gcataggcaa acgtgattca gtaaaatata
     2881 agaaattttg tttcggttga tgcggattcc aaatcgttcc acctaaatcc acataagata
     2941 ttttcttaaa cttatatcta aagttcaaac ccaaaccaaa gtttgcaaat gaaacattat
     3001 tatttgaaaa tgtttcacca gtcgcagaac caggcacata acctgtaccc acaatatatt
     3061 gattatcaaa agtaagtctt ccataatcca acgattttat cgtcataccg gcagtagcac
     3121 caaagctagt ccaaaacctt cctttggcaa aacccttgga aactgccagc gtaatattcg
     3181 gattaaatgt acttaacaaa ccatcaccgg ctctgtcaaa caagaaactc aatccgccgc
     3241 cgaaacgaaa atatttttga ggtttacaaa taaaacaacg atcataggaa ccccatgccg
     3301 tcataaaagg caccggcaca gtacgccatt gatctctgta cataccaaca aagcgattgt
     3361 ctttatcgcc aaatcctgcg taagaaggat tgagatacag gggattatac tggatttgtg
     3421 aaaagtgagg atcttgagca tttatgctat tccaaacaca tgcaaaaaag agtccaagaa
     3481 agaaaagctt tttcatattg ctaatatagt tatttatttg ttatcttaat actgttacgt
     3541 tacctttttt tgtaataatt gttccatttg aacattcacc cctgataatg aagccaaaaa
     3601 catcagcctg aaccagctta cctttataag ttccgtccca acctgtgccc aaatcgtttg
     3661 tttcaaaaac gacagtaccc cagcgatcat atatcagcat agacatagat tttagcaagg
     3721 gtcctctcac tataaataaa tcattttttt cgtccccatt tggagtaaag gcgttaggaa
     3781 tatacaaatt atcgaaagta cattcaattt ctttcaattc aattagaata gaatctgttg
     3841 ttgcacatct cgtattgctt ccggttactg ttacatagaa ccaagtagtt tctataatag
     3901 ttgctgctgt tatactatct gtaggtgagg ttacattact tacaggtgtc cagttgaaag
     3961 tagtagccaa aggagatgat gcgtgcaatt ggaattcttg tcctgctacc ggattggtca
     4021 aactagttga aatagtgaca tccggaccat caatgactgt caagtctaaa ttaaccgtgc
     4081 tatcacatcc ggctgaagac tgcagaattg catgatgcat gccagcatta gtaaatgtac
     4141 tatcaccaat tacaacctcc tccccacgac atattattct gtttatattc gatacaagcg
     4201 gttctgatac agataggttt aaagtaatta tactatcgca acccgattga ttttgaacag
     4261 taaccgtgta tgttccggaa gcattaaaag tttggcttcc aatcgtgtaa ctgtctccgg
     4321 cgcaaattga cttattaatg gtggatgtta gtggtggatt tactgtcaag tttaatgtaa
     4381 caatactatc acaagaactt gcagcggtaa gtgtaataaa gtaagttcca ctttgattaa
     4441 atgtttgcgt tcctatggta tatgactgtc cgctgcaaat tgtagtattc actgttgatc
     4501 tggattttgg tgtaacctgt aaaacgaggt ttacaatact gtcgcattgt gcagatgtat
     4561 agaaatgctg aggataagta cctgcctcca tatacttaat accgttataa atcaatgaat
     4621 caccttcaca aattgttctt gtatcttcaa ttaaatacgt tggattaacg gataaattca
     4681 aggtaatgat actatcacaa agcacactat tttgaagtgt aacggtatac gtaccacttt
     4741 gattgaaagt ttgtgtgcct actgtatatg tatcaccctg acaaattgcg gtattaattt
     4801 cagtacgtga aacaggattt actgttaggt ttaaataaac agaactgtca catcctgcag
     4861 agttggtaaa atgtacggta taatttccgc tttggtcaaa tgtctgactt cctaaggtat
     4921 agctacttcc ttcacaaatc gttttttcta attgggaaga tgttggacta tttacaacca
     4981 aatgcaggtg cactatactg tcgcaccccc ctgtagtttg caaaacaaca gtgtaatcac
     5041 cggatgcatt aaatgtttga gttccgatag ttacactttg accttcacaa attgaattat
     5101 caatgtttaa agttaatgca tcagttactg taaggtttaa tgttgaaaag ctatcgcaac
     5161 catatgcaga agtcaatgta tcaaaatatg ttccggattc agttaattct tgtccattaa
     5221 aagtatatga ttgaccttga caaatggtta tatcagttga cgttctaata attgcattca
     5281 ctacaatact catggttgtt gttgtggcac attgtcctgc tgttggag
//
LOCUS       DAOQII010000178         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9035_length_5297_cov_3.636971, whole genome shotgun sequence.
ACCESSION   DAOQII010000178 DAOQII010000000
VERSION     DAOQII010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9035_length_5297_cov_3.636971"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..1120
                     /locus_tag="PLF48_08655"
     CDS             488..1120
                     /locus_tag="PLF48_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49435.1"
                     /translation="MSKYLKIRILPFFTFFLTTFIVGISSCNHNKQSSPADINAESNL
                     LPLLIKQSLIGKELFNWGKVEDKQIVNCRILNANSENNQVNCTAVLDLDNGAKNKPGY
                     FFDCSFKDGQILSFIINKIEYNNPLAVGDFDAYSFRPLAGCKIYISTFGKKILVRTCS
                     NCDFNAISTKEEEMKEIEVADIISIKATDKSTDRIRFTYIPVSQQQIQVQ"
     gene            1120..2805
                     /locus_tag="PLF48_08660"
     CDS             1120..2805
                     /locus_tag="PLF48_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNF49436.1"
                     /translation="MRRKLFSYLFLYLLTSFHVAGQPPVDPKTGKEIYKDKHDTKAKA
                     KDKENKQKDSNQLPQSDTKTQKTANKTELKKDAVQVITEPVLPDRVDEIYIDPIEDFQ
                     RIHFHYKGSEESELLGDSVIIGMKLEKHTSYILQIRQEEYIHPEAKTNAIRYILVKQI
                     FPETTFSVSNPVTPIDVYPVDESIILNIDYRLDDNNMLRIKQSGDESYIAYNGKIFGF
                     DYNENHLYIIKVEKVGSDYKLNKIESDKYVGVIIPEPKNKSFPKFLEYDDPSTGQSVS
                     NIYSPNTENIRVDYLTENNHIRIRKENEGKYSEFFWSIFGFTYTEGHSYMLEVEKYGM
                     NYKLVKVNNDTKQDFFNSNSFLVIHFPINEYGKAEIETRFNSNGFAPANFKDENRENN
                     QESYQNNIKKQVDFNSYQNNTDNADKKSTTTDIIPQEQNTQTKENPLSKLKPKEIIND
                     KEDEVIYERTDENPVFPGGKVELMRFIENNLVYTDEIINNKIEGKVTVKFIIDKEGSI
                     NNPIVVKDISGCASCSDEAKRVCYKMPKWSPGINNGQAVKSYYYLQIFFRKKE"
     gene            2998..>5297
                     /gene="secA"
                     /locus_tag="PLF48_08665"
     CDS             2998..>5297
                     /gene="secA"
                     /locus_tag="PLF48_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794846.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNF49437.1"
                     /translation="MLNFLQKGLSKIFGSKSSRDVKTLEPIVQQVNLYFEQFQILNND
                     ELRNKTQDFKARIKEYLADIDAKIAELSASAENDDLELEQKEAIFTEVDKLKKQRDEA
                     IEEVLEEILPQAFAVVKETARRFSTNETLTASATQLDRDLAVKNKFIQINGDKVSYPN
                     SWLAAGGEIKWNMVHYDVQLLGGMVLHRGKISEMATGEGKTLVSTLPAYLNALAGCGV
                     HIVTVNDYLAKRDSEWNGLLFNFLGITVDCIDKHEPNTDARRNAYLADITYGTNNEFG
                     FDYLRDNMVHDASQQVQRKLHYAIVDEVDSVLIDDARTPLIISGPVPRGEEQMFYELK
                     PRIERLVSAQKKMVQDFLVTAKKQIAEGNTGVKEGEGGLALLRAHRGLPKNPALIKFL
                     SEVGVKQILMKTENYYLQDQQKEMPNCDRELYFTIDEKNNQIDLTEKGIELITASGEE
                     KDFFVMPDIGADIAEVENADLTHEEKLEQKDEVLKNFAIKSERLHTVNQLLKAYCLFD
                     KDVEYVIIEDKVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKVEAATQTFATITLQN
                     FFRMYHKLAGMTGTAETEATEFWEIYKLDTVVVPTNKATSRDDKEDLVFKTKREKYNA
                     IIEEIGRLKDAGRPVLVGTTNVEVSELLSRMLNMRKIPHNVLNAKQHAREAEIVAEAG
                     KNGQVTIATNMAGRGTDIKLGLGVNDGGGLAIIGSERHDSRRVDRQLRGRAGRQGDRG
                     STQFYVSLEDELMRLFGSDRIAGLMD"
BASE COUNT         1872 a          861 c         1037 g         1527 t
ORIGIN      
        1 gcaaacagta aatcttgctc ttctaaaact tttttaccat ttacatatac accatctttt
       61 agttcagctt caaatctttt taaattttct ggtgaattaa caaaatcaaa actatcactt
      121 tgcaattgga aattaattca tcaataacgc cattaaaacc attgcttcca taatacccag
      181 cattgcttac tgaattgtct aaataaaaat tcactctccc tatttcaact gctttttcag
      241 tattctcatg tttcgggtca cttttacaag atgtgcaaaa cattagtgcc aaaataaatg
      301 cgcttaatat gaagagaaaa gataatggtt ttttcataat gaggatattg cgactaatat
      361 atgagtttat tcacatttct ccaactatta agcaaaaaaa aattctattt tcttaaaaaa
      421 tctgtcttat tttgaagaat ctttctatta ttgtatcttt atacctcgct tgttttacct
      481 atattttatg agtaagtatc tgaaaattag aatattacct ttttttacct tttttttgac
      541 gacgtttatt gtaggcatct cgtcatgtaa tcataataag cagagttctc cagctgatat
      601 caatgctgaa agtaatttgc tacccctcct tattaaacaa tcacttatag gtaaggagtt
      661 atttaactgg ggtaaagtag aggataaaca aatcgtaaat tgcagaatac tgaatgcaaa
      721 ttctgaaaac aatcaagtga actgtactgc agttttagat ttagacaatg gggcaaaaaa
      781 taaacccggt tatttttttg actgcagctt caaagatggg caaattctgt catttataat
      841 taacaagatt gaatataaca atccattggc agtaggggat tttgatgcat actcattcag
      901 accattagca gggtgtaaaa tatatattag cacttttggt aagaaaatac tggtaagaac
      961 atgctctaat tgtgatttca atgctatttc cactaaagaa gaggaaatga aagaaataga
     1021 ggttgcagat attatttcga taaaagcaac ggataaatca actgacagaa tccgatttac
     1081 ctatattcct gtttcacaac aacagattca agtacagtaa tgagaagaaa attattttca
     1141 tatttatttc tttatctttt gacttccttt catgtggcag gacaaccacc ggttgaccct
     1201 aagacaggaa aagagattta taaagataag catgacacaa aagcaaaagc aaaggataag
     1261 gagaataaac agaaagatag caatcaactc ccccaatcag acactaagac tcaaaaaacc
     1321 gccaataaaa ctgaacttaa aaaggatgca gtgcaggtaa ttactgaacc tgtattacca
     1381 gatagggtag atgaaattta cattgatccg attgaggatt ttcagcgtat ccattttcat
     1441 tataagggga gtgaagaatc tgagctactg ggagattctg ttattatagg aatgaaactg
     1501 gaaaagcata caagttatat tttacaaatc aggcaagaag aatatataca cccagaagcc
     1561 aaaactaatg caatccgtta cattttggta aaacagattt ttcctgaaac aactttttca
     1621 gtaagtaatc cggttacacc cattgatgtt tatcctgttg atgaaagcat tatactaaat
     1681 attgattaca gattggatga taataatatg ttgcgcataa aacagtcggg ggatgaatct
     1741 tatatagcct ataatggcaa aatctttgga tttgattata atgaaaatca tttgtatatt
     1801 ataaaggttg aaaaagtggg tagtgactac aagttaaata aaatagaaag cgataaatat
     1861 gtaggagtaa ttattcctga acctaaaaac aaaagttttc caaagttctt agaatatgat
     1921 gatccatcaa cagggcagtc tgtaagtaat atatacagtc caaatactga gaatattaga
     1981 gttgactatt taacagagaa caatcatata agaattagga aagaaaacga aggaaaatat
     2041 tctgagtttt tttggtccat ttttggattt acctatacag aaggacacag ttacatgtta
     2101 gaagttgaaa agtatggcat gaattataaa ctggtcaaag tgaacaatga tactaaacaa
     2161 gactttttta actcaaattc atttttagtt attcactttc caataaatga atatggaaag
     2221 gcagaaattg aaactcgttt taatagcaat ggttttgcac cagctaattt taaagatgaa
     2281 aatagggaga ataaccagga aagctatcaa aataacataa aaaaacaggt tgattttaat
     2341 tcgtatcaga ataatactga caatgctgat aaaaagagta caacaacaga tataattcca
     2401 caagagcaaa atactcagac taaagaaaac cctttgtcta aacttaaacc taaagaaatt
     2461 attaatgata aagaagatga agtcatatat gaacgaactg atgaaaatcc tgtttttccc
     2521 ggaggaaaag tagagctcat gagatttatt gaaaacaatc tggtctatac cgatgaaatc
     2581 attaacaata aaattgaagg aaaggttact gttaaattta taatcgacaa agaaggcagt
     2641 ataaataatc caattgtagt taaagatatc tcaggttgtg cttcatgctc tgatgaggcg
     2701 aaaagagttt gctataaaat gcccaaatgg tcacccggca taaataacgg gcaagcagta
     2761 aagtcatatt actatctgca aatctttttt agaaagaaag aatagatttt cagtttatgt
     2821 tgttgtaatt ctataaatat atatttactt actactttcg aatcacaact tattgccagc
     2881 ctttatccgc cattttgtcg tactgttatt tttagcatat attttgctga ttcacgttta
     2941 ttagcactcc aaatggggct aaaatgctaa atttgcaatc tattttaacg acatatcatg
     3001 cttaatttct tacaaaaagg gctttctaaa attttcggca gcaagtcctc ccgcgatgta
     3061 aaaacactgg aacccatcgt acaacaagta aatttgtatt tcgaacagtt tcaaatactg
     3121 aataatgacg aactcagaaa taaaacgcag gatttcaaag cccgtattaa agagtattta
     3181 gccgatattg atgccaaaat tgccgaattg tcagctagtg ccgagaatga tgatttggaa
     3241 cttgagcaaa aagaagcaat ttttactgaa gtagataaac tcaaaaaaca acgcgacgaa
     3301 gctattgaag aggtgttgga agaaattttg ccccaagctt ttgctgtggt taaagaaacc
     3361 gctcgccgtt tttcgaccaa tgaaactttg actgcttcag ctactcagct tgacagagat
     3421 ttggccgtaa aaaataaatt tatccaaatc aatggcgata aagtgagcta tccaaattcg
     3481 tggctggctg ccggtggcga aataaaatgg aatatggtgc attatgatgt gcaattgcta
     3541 ggcggtatgg tgttgcaccg cggcaagatt tcggaaatgg cgacaggtga agggaaaacc
     3601 ttggtttcca ctttgccggc atacttaaac gccttggcag gatgtggtgt acatattgta
     3661 acggtgaatg attatctggc taaacgcgac agcgaatgga atggtttatt gttcaacttt
     3721 ttgggtatta ctgtagattg tattgataaa catgaaccga ataccgatgc cagaagaaat
     3781 gcatatttgg cagatattac ctatggtacg aataatgagt tcggctttga ttatttaaga
     3841 gataatatgg tacatgatgc cagccagcaa gtacaacgca aattgcatta tgccatcgta
     3901 gatgaggtcg attcagtact tattgacgat gctcgtacac ctttgattat ctcaggacct
     3961 gtaccccgtg gcgaagaaca gatgttttat gaactcaaac cacgtataga acgactggtt
     4021 tccgcacaga aaaaaatggt acaggatttt cttgtaaccg ctaaaaaaca aattgctgaa
     4081 ggtaataccg gtgtaaaaga aggcgaaggc ggactagcct tgttgcgggc acatcgggga
     4141 ttgcctaaaa atccggcact tatcaaattt ttgagtgaag tgggagtgaa gcaaatcttg
     4201 atgaaaaccg aaaactacta cctgcaagat cagcaaaaag aaatgcctaa ttgcgaccgg
     4261 gaactttatt ttaccataga tgaaaaaaac aatcagattg acttaacgga aaagggaatt
     4321 gagttgatta cggcatcggg tgaagaaaaa gatttttttg tgatgccgga tatcggagca
     4381 gatattgccg aggtggaaaa tgcagattta actcatgaag aaaaactgga gcaaaaagat
     4441 gaagtattga aaaacttcgc cataaaatct gaacgcttac acacggttaa ccaattgctg
     4501 aaagcttatt gtttgttcga taaagatgtg gaatatgtca ttatagaaga caaggtaaaa
     4561 attgtagacg agcagacggg tcgtattatg gaaggcagac gttacagcga tggccttcac
     4621 caggcaattg aggctaaaga aaatgtaaag gttgaagcag ctacccagac ttttgcaacc
     4681 attaccttgc aaaatttctt ccgtatgtat cacaaattag caggtatgac gggtacagct
     4741 gaaacggaag caacagagtt ctgggaaatc tataagttag atacggtagt tgtgcctacc
     4801 aataaagcga catctcgcga tgataaagaa gacttggtat tcaaaaccaa aagagaaaaa
     4861 tacaatgcca ttattgaaga aataggcaga ctgaaagatg ccggtcgccc tgtattagtg
     4921 ggtacgacga atgtggaagt gtctgagctt ttgagccgga tgttgaatat gcgcaaaatt
     4981 cctcacaatg tactcaatgc caagcaacat gcccgtgaag cagaaattgt agccgaggcc
     5041 ggtaaaaacg gacaagtaac aattgctacc aatatggctg gtcgtggtac ggatataaaa
     5101 ttgggattag gtgtaaacga tggcggcggt ttggcaatta tcggttcaga aagacacgac
     5161 tcccgccgtg tggacaggca gttgcgcggt cgtgccggtc gtcaggggga cagaggttct
     5221 acccagtttt atgtgagcct agaagatgag ctcatgcgct tgtttgggtc tgaccgtatt
     5281 gcaggcttga tggacaa
//
LOCUS       DAOQII010000179         5252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9151_length_5252_cov_4.484895, whole genome shotgun sequence.
ACCESSION   DAOQII010000179 DAOQII010000000
VERSION     DAOQII010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9151_length_5252_cov_4.484895"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLF48_08670"
     CDS             <1..359
                     /locus_tag="PLF48_08670"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49438.1"
                     /translation="NFFANGFRHAHDHISGNNQWTERTVVKKDFAVPYVMAMPGIMQS
                     HYFNTSTRELKCAFLTNDSEYGPVVYINPDWYPDGYSVYVNGIKTGTEADTYHRVSIA
                     WSSANSGGILLEVKPD"
     gene            complement(356..979)
                     /locus_tag="PLF48_08675"
     CDS             complement(356..979)
                     /locus_tag="PLF48_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF49439.1"
                     /translation="MNEINTTKICSHYKKGDSLFKEGSYASGIYCINNGKIKVSKIGE
                     EGKEQIVRLAKPGDIIGYKALLTGDRYSATAIALEDSNVCFIPREIFLNVLQKDAALS
                     FEMMKILSNELKSAEEKIMHLAQKPVRERVAETILFLRETYGIDADNNINIALSREDI
                     ANLVGTATETTIRLLSEFNKENIIELNGKKIKIVNNNKLVKTANLYD"
     regulatory      1260..1449
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1475..3454
                     /locus_tag="PLF48_08680"
     CDS             1475..3454
                     /locus_tag="PLF48_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNF49440.1"
                     /translation="MKRLIYISLFLGWNTFSSIFSFSQSVIYPDTINGGVLDSVLIND
                     NKSEPDLLTIIHNFSRVEHLIPEKYVKQIPLSFMEMLQRYSSLQVNEYGMGNIASLTY
                     RGAADDHTKIMWNGVVLNSASLGGTDLSLIPVASINQILLENNVNSIGGVLDLQTSAD
                     WNNKYGLNFQSDIGSFDAFKNSMSLYAGNKKIQYHATGIYQTAKNNFPYYDVYKDGMP
                     LDTVSHNKLKLWSASNEFFIKLSKNSELAFGNLLLSKDKQIPQLMGIFAASSKYQEDL
                     DSRTYLRYTGKLRKRIMLELLLAHSFEQLLYQDKFHPDDTIYSVNTLAKTNRLTHSFL
                     VRKDFSKYYLQLTTGYNYSFLSGNVPQYANVAKEHQGQVFGNLYFQKRFWASGIKIEF
                     PFTSYKIIRPQFSIHVALVNLKNKYDLKISYKDTYRFPDLNDRYWSPGGNPDLKYESG
                     WELELRHALHLIKSEKLRFNFSTLCYYAQIKNNIVWLPVNAVVWTPQNIAAVRRYGLE
                     NEFDLSIAPIMKFTWNLSGIYLFNRTQIIEDENNADLKNKYLAYRPQHIFKLNTDFDF
                     QYFDLGLNYSYTSFRFTDKENYSFFSLKPYHLVNMFLKFSGTYKSFSGAFVFKINNLT
                     NTQFESVRAYAQPLRNYSISILLSFQNNLKNENTQ"
     gene            3438..4520
                     /locus_tag="PLF48_08685"
     CDS             3438..4520
                     /locus_tag="PLF48_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49441.1"
                     /translation="MKTLSKLKLVVISCVILIACNKEKSTIITNYVEPGSAFVINEGN
                     FGHNNGNISYISHNKNVVNGIYETANRPSVLGDVIQSFERVGNTGIICANNSNKVVMV
                     DVSTFKQTAVITQSITYPRYALGINSNKAYISNWSFPGLLAVIDLNTKSVIKTITVGN
                     GPEEMIQASGKVFVANSGGFGNDNTLSIINPGSDVEEEKVTVRDYPSEMVLDAQGFIW
                     ILCKGHVEYPAPDYYPHRLSASYLVRFNPATKTIVKEIMLIPASADFSSSDNLAINGT
                     GTQLYFSVDDKIYTIPVTATQLPAQAFLQKYAYGLNVHPYTGDVWVCDAKDFASSGTI
                     YRYNQSAVAQDSFKVGLIPNSIYFNE"
     gene            4577..5134
                     /locus_tag="PLF48_08690"
     CDS             4577..5134
                     /locus_tag="PLF48_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNF49442.1"
                     /translation="MRIISGSLRGKRFYPPKNIPARPTTDFAKSALFNILQNHIDFEK
                     VSFLDLFAGTGSLDMEFFSRGCTDITSVDMSSISIGFIKKMSEEVKIPNHKIIKGDAL
                     HYIRTCRQQYDVIFAGPPYALESINEIPDMVIDGGLLKPMGIFILETSPAHNFLNHKH
                     LKDVRHYGQTHFWFFQPEINENIQA"
BASE COUNT         1735 a          864 c          935 g         1718 t
ORIGIN      
        1 ataatttttt cgcgaatggc ttcaggcatg ctcatgacca tatcagtggc aataatcaat
       61 ggacagaacg aacggtagtc aaaaaagatt ttgccgtacc ttatgtaatg gcaatgccgg
      121 gcatcatgca gtctcattat ttcaatacaa gcacacgtga gttgaaatgt gcctttttga
      181 ccaatgatag tgagtatggt cctgttgttt atatcaaccc ggattggtat cccgatggtt
      241 attctgttta tgtaaacggg ataaaaacag gaactgaggc tgatacttac catagagtaa
      301 gcattgcgtg gagtagtgca aattcaggtg gaatattgct ggaagtaaaa ccggattaat
      361 cgtataagtt agcagttttc actaatttat tattattgac aatttttatt ttcttgccat
      421 tcagttcaat gatgttttcc ttattaaatt cagataataa acgtatagta gtctccgttg
      481 ccgtacccac cagattggca atatcctcac gagataatgc aatattgata ttattgtcgg
      541 catcaatgcc atacgtttca cgcaaaaata agatggtttc agcaactctt tctctaacgg
      601 gcttttgtgc cagatgcata attttttctt ctgcactttt cagctcatta gacaatattt
      661 tcatcatctc aaaagaaaga gcagcatctt tctgcaaaac attcaaaaat atttcacgag
      721 gaataaaaca aacattactg tcttccaaag ctattgccgt agcactgtat ctgtcgcctg
      781 taagtaaagc tttgtagccg atgatatcac cgggtttggc aagacgaact atttgttctt
      841 ttccttcttc gccaatttta gagaccttaa tttttccatt attaatacag taaattcctg
      901 atgcgtaaga accttcttta aaaagagaat ctcctttttt ataatgcgaa caaatttttg
      961 tagtgttgat ttcgttcatt ctgtcatatt ctgttttgca aaaaacagaa ttgaatctat
     1021 tggaacaatg atgacaatcg atatgctcaa aagctttcat agggaatata agtgcgaata
     1081 ttgggaaaaa aacggaatta aacaattttc agtagaataa attatgatta atatcagctt
     1141 ttttgtggat atctgaatga ggaattttta taaaaatttg aagctgaata tgtatagcat
     1201 caattatttg atgactatta tattgtaatg aaaacttagt ttgtgttgct tattttaatg
     1261 tatctttgtt tcacaaaggt tctttttagt tattgctaaa cagattaaaa gggaatcggg
     1321 tgtaaatccc ggacaatccc agttgctgta aatacacgca tggcgtactg caaataccac
     1381 tgttcttcgg aatgggaagg tgcagtaaaa gtataagtca gaagacctgc ctttgattat
     1441 caaaaaattt aagtgttctg aggaaacacc aaatatgaaa agattaattt acataagcct
     1501 gtttttagga tggaatactt tttccagcat attttcattt tcacaatccg tcatttatcc
     1561 agataccatc aacggaggtg ttttggattc agtattaatt aatgacaata agagcgaacc
     1621 tgatctttta actatcatac ataattttag tcgggtagag catttgattc ccgaaaaata
     1681 tgtaaaacaa attccattaa gttttatgga aatgctccag cgttattcca gtttacaggt
     1741 taatgaatat ggaatgggaa atattgcatc cctcacatac cgtggtgcag ctgatgacca
     1801 cacaaaaata atgtggaatg gtgtagtgct aaattcagca tcattgggag gtactgattt
     1861 atcgcttatt ccggtagcat caataaacca aattctactt gaaaataatg tcaactcaat
     1921 aggcggagtt ttagatttgc aaacaagtgc agactggaat aataaatatg gtttgaattt
     1981 tcaaagtgat ataggctcat tcgatgcgtt taaaaattcg atgagcttgt atgccggaaa
     2041 taaaaaaata caatatcatg cgacaggaat ttatcaaaca gctaaaaata attttccata
     2101 ctacgacgtt tacaaagacg gaatgccatt ggataccgtt tctcataata aactaaaact
     2161 atggagtgca agcaatgaat tttttattaa actaagtaaa aacagtgaac ttgcttttgg
     2221 caacttgctt ctatcaaaag ataaacaaat tccacaactt atggggattt ttgcagcaag
     2281 cagcaagtat caggaagatt tggatagcag aacttatttg cgttatacag gtaaactcag
     2341 gaaaagaatc atgctggaat tactacttgc tcatagcttt gagcaacttt tatatcaaga
     2401 taaatttcat ccggatgaca caatatattc cgtaaataca ttagctaaaa caaaccgact
     2461 gacacatagc tttttggttc gtaaagattt tagcaaatat tatctgcaac tcacaaccgg
     2521 atataattat tcatttttat cagggaatgt accgcaatat gccaatgtgg ctaaagaaca
     2581 ccaaggtcaa gtttttggca atctgtattt tcaaaaacgt ttttgggcat ccggaataaa
     2641 aattgaattt ccttttactt cctacaaaat catcagacca cagttttcca tacatgtagc
     2701 acttgttaat ctcaaaaata aatacgattt gaagatttca tataaagata cttatcgttt
     2761 tcccgatttg aatgatcgct actggagtcc gggcggaaat ccggatttga aatatgaaag
     2821 tggatgggaa ctagaattac gtcatgcttt gcatttgatc aaaagtgaaa aattgcgatt
     2881 taatttttca acgttatgtt attatgcaca aataaaaaac aatattgttt ggttgcctgt
     2941 aaatgctgtt gtttggacac ctcagaatat tgccgctgtc agacgatatg gtttggaaaa
     3001 tgaatttgat ttatcaattg caccaataat gaaatttact tggaatcttt caggtattta
     3061 tttgtttaac cgcacacaaa ttattgagga tgaaaacaat gcggatttga aaaataaata
     3121 tttagcctat cgcccgcagc acatttttaa gctcaacaca gattttgatt ttcagtattt
     3181 cgatttaggt ttaaattaca gttatacctc attccgattt actgataaag aaaactattc
     3241 cttcttttca ttaaaaccgt atcatttagt caatatgttt ttgaaatttt cggggacata
     3301 taaaagcttc tcgggtgctt ttgtatttaa aatcaataat ctcaccaata cccagtttga
     3361 atctgtcagg gcatatgcgc agccgctgcg caactattca atttccattc ttttatcatt
     3421 tcaaaataat ctaaaaaatg aaaacactca gtaaattaaa attagttgta atcagttgtg
     3481 taatactgat tgcctgtaat aaagaaaaat caacaatcat taccaattat gtcgaaccgg
     3541 gatctgcttt tgtaattaac gaaggcaact tcggtcataa taatgggaat atatcctaca
     3601 tttctcacaa taaaaatgta gtaaacggta tttatgaaac agcaaacaga ccatcggttt
     3661 tgggagatgt cattcagtct tttgagcgtg taggaaatac gggcattatt tgtgcgaata
     3721 attcgaataa agttgtcatg gttgatgtgt ctacatttaa acaaactgcg gttattacac
     3781 aatcaattac atatccgcgt tatgcattag gcataaacag taataaagca tatatcagca
     3841 actggagttt tccgggtttg ctggctgtta tagatttgaa tacgaaatcg gtaattaaaa
     3901 cgataacagt aggaaatggt cctgaagaaa tgatacaagc tagtggaaaa gtatttgttg
     3961 ccaatagcgg aggctttgga aatgacaata cacttagcat cattaatccg ggcagcgatg
     4021 tggaagaaga aaaagttacc gttcgtgatt atccatctga aatggtactg gatgcacaag
     4081 gatttatttg gattttgtgt aaaggtcatg tagaatatcc tgctcctgat tattatccac
     4141 accgtttatc tgcatcttat ttagtccgat ttaatcctgc aacaaaaaca atagttaaag
     4201 aaattatgct tattccggct tctgcagatt tcagttcatc cgataattta gctattaacg
     4261 gtaccggtac acaattgtat ttttctgtag atgataaaat ttatactata cctgttacgg
     4321 caacgcaact gcctgctcaa gcctttttgc aaaaatatgc ctatggacta aatgtacatc
     4381 cttacacggg agatgtatgg gtttgtgatg cgaaagattt tgcttcttca ggtactatat
     4441 atcgatataa tcaaagtgcc gtagcacaag acagttttaa agtcggcttg atacccaaca
     4501 gtatttattt taatgaatag ttcagtttaa tttattctgt ctatttttgt tgtcattaat
     4561 gatgtaagaa aagtatatgc gtattatctc aggttcttta agaggcaaac gattttatcc
     4621 gccaaaaaac attccggctc gtcccactac ggattttgcc aaatcggcat tattcaatat
     4681 tctgcaaaat catatcgatt ttgaaaaggt cagttttctg gatttatttg caggtacagg
     4741 cagcttggat atggagtttt tttcgagagg ctgtacggat atcacttctg tagatatgag
     4801 cagtatcagt atcggtttta ttaagaaaat gagtgaggaa gtaaaaattc ccaatcacaa
     4861 aattatcaaa ggcgatgcgt tgcactatat acgcacttgc agacaacaat atgatgttat
     4921 ttttgccgga cctccctatg ctcttgaaag catcaatgaa ataccggata tggttattga
     4981 tggcggtttg ctaaagccca tgggaatatt catattagaa acatcaccgg cacataattt
     5041 tttaaaccac aaacatctca aagatgttcg acattatgga caaacgcatt tttggttttt
     5101 ccaaccagaa ataaatgaaa atattcaagc ttaatattag cgaataattg aacttaactt
     5161 aactcacttg caatagaagt ttagttacat ttggttttta taaacctgag ttcgggttaa
     5221 gaaaattaag gtgaaaaaaa tagttgattt ct
//
LOCUS       DAOQII010000180         5248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9161_length_5248_cov_4.861544, whole genome shotgun sequence.
ACCESSION   DAOQII010000180 DAOQII010000000
VERSION     DAOQII010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9161_length_5248_cov_4.861544"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLF48_08695"
     CDS             <1..1157
                     /locus_tag="PLF48_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49443.1"
                     /translation="VSLGCKKLNTTAGVVITASHNPPSYNGFKLKSNFGGPTIPADIA
                     IVESLIPEKTEIPKTSLEEWQAKGLFEYHDLEKMYIDHVEASFDMDAIRNCGMIFGYD
                     AMYGAGQNVVRKLLPDNLVYLHCEYNPGFHGQAPEPLHKNLLEFSELIKHSTDIEFGF
                     ANDGDADRIGCYDDEGHFIDSHHVILLLIHYLHKYKGMSGKVVISFTVSEKIKNLCAY
                     YKIPVEVTAVGFKYICEIMVNEDVLVGGEESGGIAIKGHIPERDGVWMALVLMEFMAK
                     TGKSIKELIQEVYDIVGSFDYDRYDLHISEEIKQKVITHCKNDDYKAFGKYTIERKED
                     LDGWKYHLPNEAWTMIRASGTEPVLRVYGEAKDRAAVVELLDTVKGVLLG"
     gene            complement(1165..1626)
                     /locus_tag="PLF48_08700"
     CDS             complement(1165..1626)
                     /locus_tag="PLF48_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49444.1"
                     /translation="MNEEFKVAYDIRFDNSKNEVLNYKEEDIRKIITTDKEFEKIFRC
                     PFWIAMDSARYCKFIPELIMKLTDTTTVGLSDAGDVTIWCRVKSKQLKQNSLNYQIDD
                     DAFRICGRASWILKRLTKNEFGIISCNPTKAELIEIQKKWIKWFNNSLKVK"
     gene            complement(1751..3019)
                     /gene="ricT"
                     /locus_tag="PLF48_08705"
     CDS             complement(1751..3019)
                     /gene="ricT"
                     /locus_tag="PLF48_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNF49445.1"
                     /translation="MSCNGCGTIGGMPKGCKNNGHCATGGCSKLEVHDWLSDITYDDY
                     ARCTIFEVSFKNGSRKGFYRNDKNLDVQTGNYVIVEAAQGYDLGQITLSGELVRLQMR
                     KKRVREDSEDIKRIQRIAKESELTLMHEARAKEKETMLKARVLARNLGLDMKLGDVQY
                     QADGKKATFYYFADGRVDFRELIKVLASEFKIKIEMRQIGARQEAGIVGGIGSCGREL
                     CCSTWMTSFKSVNTAAARYQNLSINQTKLSGQCGRLKCCLNYELDTYLDALRFVPENA
                     EKLKLQDGEARLMKTDVLKGVMYYQINHSGTFYPISFENVKMVLDMNAQGKFPTDLTE
                     LAEEEVKKSKDEDVLEFADVVGQISLASLDKKDKNKRHKHHGNKQSKDSIRQNQNQQN
                     QQNKRPRNENKGNNPHHQNKPRPNNPEKPK"
     gene            3651..4058
                     /locus_tag="PLF48_08710"
     CDS             3651..4058
                     /locus_tag="PLF48_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49446.1"
                     /translation="MEFTQKFRDAVESFQTEAKTNLEELKYNVENTYKNAVSEGKEEV
                     EILKNHFKAASERIQAKLKGPFTMEKVQSDLKEEADILVADVKTTFNRNRDRVNGLVG
                     SFTQKANHVAKEVKKEVKTATSKAKTASKKAQA"
     gene            4130..4378
                     /locus_tag="PLF48_08715"
     CDS             4130..4378
                     /locus_tag="PLF48_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49447.1"
                     /translation="MRIAGYIDHPIIKITVLQLSERYAIKFEWNMLEQTYKFHDDDRL
                     RTFADVEKLVDEQFLQEVIERFKSMVQTKMESYKRNIM"
     gene            4835..5014
                     /locus_tag="PLF48_08720"
     CDS             4835..5014
                     /locus_tag="PLF48_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49448.1"
                     /translation="MVFDPIDFFKNECANTELLTPYAKKDYLGVTNTLRLKKKKPAPS
                     ELEQQHITLSERIKN"
BASE COUNT         1735 a          918 c          905 g         1690 t
ORIGIN      
        1 tggtttcttt gggttgcaaa aagttaaata ccacagccgg agtggttatt acagcttcac
       61 ataatccgcc tagttataat ggatttaagc taaaatcaaa ttttggtggg cctaccattc
      121 ccgccgatat tgctatagta gagagtttaa tccccgaaaa aacagaaatt cctaaaactt
      181 cattggaaga atggcaggct aaaggtttgt ttgaatatca tgacttggaa aaaatgtata
      241 tcgaccatgt ggaagcttca tttgacatgg atgcaatcag gaactgcggt atgattttcg
      301 gttatgatgc catgtatgga gcaggtcaaa atgtggtaag aaagttattg cccgataatt
      361 tggtctatct gcattgcgaa tacaaccccg gatttcacgg tcaagctccg gagcctttgc
      421 ataaaaattt gctggaattt tctgagttga taaagcattc cacagatatc gagtttggat
      481 ttgccaatga cggcgatgcc gaccggatag gttgctatga tgatgaaggt catttcattg
      541 attctcatca tgttatttta ttactcattc actatttgca taaatacaaa ggaatgagtg
      601 gaaaagtggt tatttctttc acggtttctg aaaaaatcaa aaatctgtgt gcatattata
      661 aaattccggt agaggttacc gctgtcggat ttaagtatat ctgcgaaatt atggtgaatg
      721 aagatgtgct ggtgggtggt gaagaaagcg gcggtattgc cattaaaggt catattccgg
      781 aacgagacgg tgtttggatg gcattggtgc ttatggaatt tatggcaaaa acaggaaaat
      841 ccattaaaga actgatacag gaagtttacg acatcgtggg ttcttttgat tatgaccgct
      901 acgatttgca tatttcagaa gaaataaaac aaaaagtgat aactcattgt aaaaatgatg
      961 attataaggc ttttggtaaa tatactattg aacgcaaaga agatcttgac ggctggaaat
     1021 atcatttgcc aaatgaagcc tggacgatga taagagccag tggaactgag ccggtattgc
     1081 gtgtatatgg cgaagccaaa gacagagctg cagtggttga gttattagat acggtaaaag
     1141 gtgtcttatt gggataaaat cagcctattt caccttcaat gaattattga accatttaat
     1201 ccatttcttt tgaatttcaa tcaattctgc tttagtagga ttgcaagaaa taattccaaa
     1261 ctcatttttc gtcagtcttt ttaatatcca actggctctg ccgcaaattc gaaaagcgtc
     1321 gtcatctatt tggtaattga gcgaattttg tttcaattgc ttgcttttta ccctgcacca
     1381 tatcgttaca tctccggcat ctgaaagtcc aactgttgtg gtatctgtca atttcattat
     1441 cagttcaggt ataaacttac aatagcgggc actgtccata gctatccaaa acgggcaacg
     1501 aaatatcttt tcaaattctt tgtcagtggt aattatctta cggatatctt cttctttata
     1561 gttcagcact tcattttttg aattgtcgaa gcgtatatca taggcaactt taaattcttc
     1621 gttcacaatt tttatttgct ttctgaattc ccatggcatt ttggaaacag gcaggtaatg
     1681 ttttaaactt aaagtttgag cagatacact taatgagctt ataatcaata aaataaaaaa
     1741 caaaaacaaa ctattttggc ttttccgggt tattcgggcg aggtttgttc tggtggtgag
     1801 gattatttcc tttattttca tttcttggtc gcttattttg ttgattctgt tgattttgat
     1861 tctgtcttat tgaatcttta ctttgtttat ttccgtgatg tttgtgtctt ttgtttttat
     1921 cctttttgtc cagcgaagct aaagatattt gacctaccac atccgcaaat tccaatacat
     1981 cttcatcctt agattttttc acttcttctt ctgccagctc agttaaatca gttgggaatt
     2041 taccttgtgc attcatatcc aataccattt tcacgttttc gaatgaaatg ggataaaaag
     2101 taccgctgtg attaatctgg tagtacatca cccctttcag tacgtcggtt ttcatcaggc
     2161 gagcttctcc atcctgcagt ttcaattttt cagcattttc cggcacaaat cgaagggcat
     2221 caagataggt atccaactcg tagttcaaac aacattttag tcggccacac tgtccactca
     2281 acttagtttg gttgatagac aaattctgat aacgggctgc agccgtattt accgacttaa
     2341 agctcgtcat ccaggtagaa cagcacaact ctcttccgca agaaccgatt ccgccaacaa
     2401 tacctgcctc ttgacgggct ccaatttgac gcatttcaat cttaatctta aattcggaag
     2461 caagaacttt aattaactca cgaaagtcaa ccctgccatc agcaaaatag taaaaagtag
     2521 cctttttgcc gtcagcctga tattgaacat cgccaagttt catatccaaa cccaaattgc
     2581 gtgccaatac acgcgctttg agcatggttt ctttttcttt ggctcttgct tcatgcatca
     2641 aagtcagttc actttctttc gcaatgcgct ggatacgttt tatgtcttcc gaatcttctc
     2701 ttactctttt tttgcgcatt tgtagacgaa ccaactcccc gctcagtgta atttgtccca
     2761 gatcatatcc ctgtgcggct tctacaatta cataatttcc ggtttgcaca tccagatttt
     2821 tgtcgtttct gtagaaacct ttgcgagatc cgtttttaaa actcacttca aatatggtgc
     2881 atctggcata gtcatcatag gtaatatcgc tgagccagtc gtgcacttcc agctttgagc
     2941 agccccctgt tgcacaatga ccattatttt tacatccttt tggcattccg cctatcgttc
     3001 cacatccgtt acaactcatt atataagatt agttttcgtt aaaaaatatt gataaacaga
     3061 taattatatt ctgtttgatt gtaaaacata caacaagaac ataaaaacta cctatttttt
     3121 acaaaaatac gaattaatga ttgaaatccg ttttgtaaga aatgaagata tgttaataga
     3181 tactgaaata atctgctatt tttactaata tttatgttgc ttttgaataa atctctaaat
     3241 tttataattt cccttaaatt tatttatctg atctgcgtta aaaatcattc cagtacacat
     3301 taattacgga agtgtgtcaa ttcaatgcat attcaatatt taaagcatta tgttacatta
     3361 ttagaaaact actcatcaat tttgatgaaa tttcaataat ttctgaaaat ttgattttat
     3421 cgaaaatttt taatttaaca tgtccaacaa aaaagacatt acaaattaac gccattttca
     3481 taattaagct gacaatattg atttaagttt aagtttcata attctgtaac aaagcattca
     3541 actaaaatac atttaatcgt caattacaga taaccttaaa aatttgacaa gcaacagaat
     3601 caggtataac tttgcagtat caaaattatt cacaattaaa ctttttaaaa atggaattca
     3661 cacaaaaatt cagagatgct gtagaaagct ttcaaacaga agcaaaaact aacttggaag
     3721 agttaaagta caatgtagaa aacacttaca aaaacgcagt aagcgaaggt aaagaagagg
     3781 tggaaatctt gaaaaaccat ttcaaagctg ccagcgaaag aattcaagct aaattgaaag
     3841 gtccttttac tatggaaaaa gtacaaagcg atttgaaaga agaagctgac attttagtgg
     3901 ctgacgtaaa aactacattc aacagaaacc gtgacagagt aaacggttta gtaggaagct
     3961 ttacacaaaa agccaaccac gtagctaaag aagtaaaaaa agaagtgaaa actgcaactt
     4021 ctaaagccaa aacagcaagc aaaaaagcgc aagcttaatt tctgtttaaa catttttaaa
     4081 aagagccgcc aatattttac agagcggctc ttttttattt tatttgtgta tgagaattgc
     4141 aggatatatc gatcatccca ttattaaaat taccgttttg caacttagtg aacgctatgc
     4201 tataaaattt gagtggaata tgctggaaca gacctataaa tttcacgacg acgatcgttt
     4261 gcgtactttt gcagatgttg agaagttggt agatgaacaa tttttgcaag aggtaattga
     4321 gcgatttaaa tccatggtgc aaactaagat ggaaagctat aagcgaaata taatgtagtt
     4381 tatcaatggt gatatcccag ctttgcagta tatgggtatg actttacatg gatagataca
     4441 aaaaataaaa aaccaactct aacccaaaaa tcaaagaata gtaaaaacta ttccatatac
     4501 ttcagattca caatcctcaa tatgcgtgct aattttatgc atagaatagc ataagcaaca
     4561 tttgcaacta ttatcactca atatttcaaa tcctgatttt taatatagaa ataaaatgta
     4621 tacattttac ttaagtatat ctcgtcctaa aataagatta cacatttata acttaaagaa
     4681 gtgtaaacat aaacaagaaa ataaacaaca aaaatgcagc gtgtgctgtt aaaaacagaa
     4741 gacaattcct agtcatttct ctgaggagtt taagaaaaaa atcattgccg aatatttcga
     4801 atccgatctg actaaatgta aattttggat tagtatggta ttcgatccaa tagacttctt
     4861 caaaaatgaa tgcgcaaata cggaattgtt gaccccctat gcaaaaaaag attatcttgg
     4921 tgttactaat actttgcgtt tgaaaaaaaa gaaaccggca ccatcagaat tagagcagca
     4981 acacatcact ctttctgaaa gaattaaaaa ttagaaaagc aactcttgga cgaaaaaatc
     5041 cgtgcagata tgtttgcccg tgtaattgaa attgctgaaa gcgaacttaa atatttcgat
     5101 tagaaaaaag tatgacacca aataatccgt gagactaaag aaatgtatta gcatgtatat
     5161 ttatatatgg atgactatca attttggtaa actattatta acctgagttc gggataagcg
     5221 tttcaattaa aaaatagttg atttttta
//
LOCUS       DAOQII010000181         5240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9182_length_5240_cov_6.101832, whole genome shotgun sequence.
ACCESSION   DAOQII010000181 DAOQII010000000
VERSION     DAOQII010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9182_length_5240_cov_6.101832"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLF48_08725"
     CDS             complement(<1..525)
                     /locus_tag="PLF48_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNF49449.1"
                     /translation="MIKTIVIYTGLILSVFLNSCKKDKNLLEQNISIRIDSIFPNEGN
                     YRTEVLIFGNNFDQLPSSAFVTLNNKHCTILSKSNTLLKLKLPERAGSGAFQIHAGAQ
                     VFSAYTFSYHFIYATSTVAGDGTIGLRNGPGNQAQFYYPVGIVKDSKGDYFITDYYNS
                     VVRKMDKNYNITTYA"
     gene            complement(959..1684)
                     /locus_tag="PLF48_08730"
     CDS             complement(959..1684)
                     /locus_tag="PLF48_08730"
                     /inference="COORDINATES: protein motif:HMM:NF040452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HNF49450.1"
                     /translation="MKNVSLFILSLFLIFGTTNFTLAQKNHGQKLNQNKQQNNKPQKQ
                     QYNKADQLKSPQQKTQGQNKTYTNKGENKSNNKNGYYHSQNVHKQVYVHHYSRNKVVV
                     VKPRVVKTYTVLPVGYTTLYWGNRNYYFHNGWYYHWHKGIYTVVPPPFGIRIKVLPIG
                     YRTIYVGGLPRYYYSGVYYQQIDNEYETVEPAEGTIVPNLPDDAEEITMDGTTYYTYD
                     DILYKPIVTNDGMQYEVAGKLDD"
     gene            complement(1757..2866)
                     /locus_tag="PLF48_08735"
     CDS             complement(1757..2866)
                     /locus_tag="PLF48_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF49451.1"
                     /translation="MKNAFLILLLSYLVFCNQHVNADPVKRYYDKMDLAKDIIDAVLK
                     NNAFRLEAITQSLVIPFTEYKSIVFPLYIEDSLATFESFPKNEVYPVSLLLKDSLNYN
                     HFIADFYFGEYIGKLLNDPDNLNPFGKNIYIAFDKEEDSISTQVNKNYLGILYETDGY
                     VKVINLEALITYKHTLYSMRQTNYHSYKKDDLPAYLFKRKLPDNIAVYKYENKKFIKY
                     TNDTLLFDDVDYSNYTNETQVSNIEENDIIPVTIDTIAYESPKESEVYDRASEMPEFP
                     GGTAEMLKYVSRSIMYPKAAKEKGLEGKVIIKFYVDTDGTVKDPVVLKDGVGGGCAEE
                     ALRIVRSMPKWKPGYDNSGSKVKVYYIIPITFKLQ"
     gene            complement(2946..3485)
                     /locus_tag="PLF48_08740"
     CDS             complement(2946..3485)
                     /locus_tag="PLF48_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49452.1"
                     /translation="MKSQRLLSLVLLIVLTVFVLSSCKYSKEPKQFKLDHLSLSYPSY
                     LQQAQDVYPSAQTIFQAKNGYRDVYFILVDWGNNSGDSMFNVISDSVVNQLKANLKEV
                     NIEKSDSSFTINDMKAREAQVSGILSQHGMDIRFLFDLVIFQTKDGHIYQTAGWLMRH
                     KQQLWLKDLQKAAFSLRIN"
     gene            complement(3534..4265)
                     /locus_tag="PLF48_08745"
     CDS             complement(3534..4265)
                     /locus_tag="PLF48_08745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNF49453.1"
                     /translation="MSVHQYQMESLPVPVKIIVETRNTSRVSLGKDAVIVRLPSHISI
                     AEKEKMVKQFLDWAKSTISQNKYYAEHTHKLDYYQNRELRIFGKSFFVRIYISTNTQH
                     KLNYRGDDQLHISLNENIDEQKQVKEIQRFLLRFSERYFLKSMQERVLYWNDVYFKER
                     IEKVVLKHTISSWGSCTAQRKITLSTKLLLMPLQVIDYVIIHELAHLKEMNHSPKFWK
                     HVEAVMPDYKLQRKWLKAYGNSVMF"
     gene            complement(4379..5029)
                     /locus_tag="PLF48_08750"
     CDS             complement(4379..5029)
                     /locus_tag="PLF48_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49454.1"
                     /translation="MMRLFVKYRFYGSIVLLIIGILIQIFVHKGYTWSIFIYIISVSI
                     ILFDIFIGPMRYLQKQVEAGDIEGAKETMSWIKFPNLLIKPVRQGYYMLKSNFAMANQ
                     NLDEAEADIRKSLASKSDLLGGSQEGLQYLQLGTIALQKGDQKEALRNLKTALQKGVP
                     DDDNLATIYLQLCTIYAQRREIKVAKEYLRRANNCKVKSEELKKHLKDINKQMARA"
BASE COUNT         1658 a          931 c          872 g         1779 t
ORIGIN      
        1 tgcataagta gttatattat aatttttatc catcttacgt acaacactat tataataatc
       61 tgtaataaaa taatcgcctt tagaatcttt tacaattcca accgggtaat aaaattgggc
      121 ctgatttccc gggccattcc gaagtcctat tgttccatct ccagcaaccg tactagttgc
      181 atatataaaa tgataggaaa aagtataagc agaaaaaacc tgagccccgg catgtatttg
      241 aaatgcaccg gaacctgccc tttcaggaag tttcaattta agcagcgtat tgctctttga
      301 aagtattgtg caatgcttat tatttaatgt tacaaaagcg gaagaaggta gttggtcaaa
      361 attatttcca aaaattaaaa cttctgttct gtaatttcct tcattaggaa aaatactgtc
      421 tattctgatg cttatatttt gttcaagtag atttttatcc tttttacacg aatttaaaaa
      481 aacagatagg attaaacctg tatagattac tattgtcttt atcatttaat aaattatagt
      541 gtttacctgt aaatttacaa aactaattca caaagaatga gtatattttt agagtaatga
      601 tatttaaaat agaagaataa aatacacaaa agacaggtta tcaatacatt acaggataat
      661 ttgtttaaaa aaagattagc atcctaaaac atgtcaaaat tcagcgaaac aataatctga
      721 cagaaatatt gtatctgttg tggtttaaga aaattcatta tttttcatca ttcgtaaacg
      781 aaaatcttaa ctaattattt taacaggggg acataaagtc aataacaaaa aataccaatt
      841 gcagtttttt ttgaaatcat atagagaaac agacttttat caaatgatga cgaatgaaaa
      901 acgaataaag ttttttcata gaaatcaaag agatcgtgct aggaaaaggt atttgtaatc
      961 aatcatccag cttgccagcc acttcatatt gcataccatc attcgtaacg atgggtttgt
     1021 ataaaatatc atcataggtg taataagtag ttccatccat ggttatttct tcagcatcat
     1081 ccggaagatt gggaactatt gttccttcgg ctggctcaac ggtttcatat tcattgtcaa
     1141 tttgttggta atatacccct gaatagtaat agcgaggcaa accgcctaca tatattgttc
     1201 tgtagccaat tggcaaaacc tttatacgaa tgccgaatgg tggtggcact acggtatata
     1261 tacctttgtg ccaatgatag taccatccgt tatggaaata ataattgcga ttaccccaat
     1321 ataaagtagt gtagccaacc ggcaaaaccg tatatgtttt taccactctg ggttttacca
     1381 ccaccacttt atttctggaa taatggtgta catatacttg cttgtgtacg ttttgtgaat
     1441 gatagtaccc gttcttatta ttagacttat tttctccttt attcgtgtac gttttatttt
     1501 gcccttgtgt tttttgttgt ggcgacttaa gttggtctgc tttgttgtac tgctgtttct
     1561 gtggcttatt gttctgttgt ttgttttggt tcagcttttg cccgtgattt ttttgtgcca
     1621 atgtaaagtt ggttgtccca aaaatcaaaa ataaagacaa gatgaataat gaaacatttt
     1681 tcataatgga tattttcttc ctttgactaa ataaaatgaa aaaagtttaa tcagaaattc
     1741 tctaaacgaa aatatttcat tgtaatttaa acgtaatggg tatgatgtaa tatactttaa
     1801 ctttgctccc actattatca tatcccggct tccacttggg catacttctt acaatgcgca
     1861 atgcttcttc ggcacagccg ccaccaacac catcttttaa tacgacagga tcttttaccg
     1921 tgccatccgt gtccacataa aacttgataa ttacctttcc ctctaagcct ttttcttttg
     1981 ccgctttcgg atacattatt gatctagaga catacttcaa catttcagct gtaccgcctg
     2041 ggaattctgg catttctgag gctcggtcat aaacttcaga ttctttgggc gattcatatg
     2101 ctatggtatc aattgtaacc ggtattatat cattttcttc aatattacta acttgtgttt
     2161 cattagtata attactataa tctacatcat caaatagcag ggtgtcgttt gtatacttta
     2221 taaatttctt gttttcgtac ttatatactg caatgttgtc cggtaatttc cttttaaata
     2281 aatatgccgg cagatcatct ttcttgtaag aatgataatt tgtttgtcgc atggaataaa
     2341 gtgtatgctt atatgttatg agcgcttcca gattaattac ttttacatag ccatcggttt
     2401 cgtataatat acccaaatag tttttattca cttgggtaga tattgaatcc tcctctttgt
     2461 caaatgcgat ataaatattt ttaccaaagg gatttaaatt atccggatcg ttcagaagct
     2521 tgccgatata ttcaccaaaa taaaaatctg caataaaatg attgtaattc aaagaatctt
     2581 taagcaataa cgacaccgga taaacttcat ttttggggaa tgactcaaac gtagccaaac
     2641 tgtcttcaat gtataaagga aaaacaatac tcttgtattc agtaaatgga atcaccaagg
     2701 attgagtaat agcttccagc ctgaaagcat tgtttttcaa tacggcatct attatatctt
     2761 ttgctaaatc catcttgtcg tagtagcgtt ttacaggatc agcattgaca tgctgattgc
     2821 agaataccag ataagataaa agtaaaatca ggaatgcatt tttcattggt gattattgat
     2881 gtttttttta taaaaatata atattcttgt ccatttagac tgaattcaac taaaaaacgc
     2941 aatttttaat ttatacgtaa tgaaaacgct gctttttgca gatcttttag ccatagctgt
     3001 tgtttatggc gcatcagcca gcctgcagtt tgatagatgt gcccgtcttt ggtctggaaa
     3061 ataacaagat caaacaaaaa gcgaatatcc ataccgtgct gtgataatat acccgatacc
     3121 tgcgcttcac gagccttcat gtcgtttatt gtaaaagagg aatctgattt ttcgatattc
     3181 acttctttca aattcgcttt gagctggttt acaacagaat cacttattac attaaacata
     3241 ctgtcacccg aattgttgcc ccagtctact aaaataaaat agacatcccg atagccgttt
     3301 ttagcctgaa aaatcgtttg ggcagaaggg taaacatctt gagcttgttg caaatatgat
     3361 ggataggaca aagacagatg atccaacttg aattgctttg gctctttact gtatttgcat
     3421 gaggacaaaa caaaaacagt taaaacaatt agcaatacaa gacttaataa acgctgagat
     3481 ttcatgttgt caaaagtaga aaaaataagc gtaacccgag aatgcatcct ttatcaaaac
     3541 atcacagagt tgccgtaagc ttttaaccat ttacgctgta gtttataatc cggcattacg
     3601 gcttctacat gcttccagaa tttgggcgag tggttcattt ctttaagatg ggcaagttcg
     3661 tgaatgatga catagtcaat aacctgtagc ggcatcagga gcaatttggt agacaatgta
     3721 attttacgtt gtgcagtaca gcttccccag ctggaaatcg tatgctttaa cactactttt
     3781 tcaatacgtt ctttaaaata aacatcgttc cagtacaaga ctctttcttg catacttttt
     3841 aaaaaatatc gctcagaaaa acgaagcagg aatcgctgaa tttcttttac ttgtttttgc
     3901 tcatctatat tttcattgag ggaaatatgc aactggtcgt ctcctctgta attgagctta
     3961 tgctgagtgt ttgtgcttat atagattcta acaaaaaaag atttgccaaa tatgcggagt
     4021 tctctgtttt gataataatc aagcttgtga gtatgttctg cataatactt gttttgactg
     4081 atggttgatt ttgcccaatc cagaaattgt tttaccattt tttctttttc tgcaatggaa
     4141 atatgactgg gcaacctaac aattaccgcg tctttaccca aagaaacacg agaagtgttt
     4201 cgggtctcca cgataatttt taccggcaca ggcaggcttt ccatttgata ttgatgcacg
     4261 ctcacctgat aaaaataagg ggttctaaat aagcggaagc taaaatttat aaacaataac
     4321 cggatattgt tatcagcaat aaaaaacagt aggtttttat attagaactt ttcctgcatc
     4381 aggctctcgc catttgtttg ttgatgtctt taagatgttt tttcagctcc tcgcttttta
     4441 ctttgcagtt gttagcacgg cgcaaatatt ctttggcaac tttaatttcg cggcgctgtg
     4501 cgtaaatggt gcaaagctgc agatagatgg tagccagatt atcatcatcc ggtacgcctt
     4561 tttgcaaggc ggttttcaaa ttgcgcaagg cttctttttg gtctcctttt tgtaaggcaa
     4621 tagtgccgag ttgcaaatat tgtaatcctt cctgagagcc tccaagcaaa tcacttttac
     4681 tggccaggct cttgcggata tctgcctctg cctcatccaa attctggttg gccatagcaa
     4741 aattcgattt cagcatataa taaccttgtc tgaccggttt tattaataga ttcggaaatt
     4801 ttatccagga catggtttct tttgctcctt caatatctcc ggcttccacc tgcttttgca
     4861 aataacgcat aggtcctatg aaaatatcga acaagataat agacacagag ataatgtaaa
     4921 taaatataga ccaggtatac cctttatgaa caaatatttg gattaagatt ccaataatca
     4981 gcaaaacgat agagccgtaa aaacggtatt taacaaacaa acgcatcata ttcataatat
     5041 aaaaattgta gcgcaaaaat agcgcaatac ctacagatat aaaaatatag gagcagaata
     5101 tctttactaa tgcgcaaata tgcaaactat gccaatttgc tataagttgc ttcttattag
     5161 taataaataa aatctctaaa acatatccgg caattttatt tatcttgcaa aataataatt
     5221 acagcataat taggaataat
//
LOCUS       DAOQII010000182         5200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9313_length_5200_cov_3.761322, whole genome shotgun sequence.
ACCESSION   DAOQII010000182 DAOQII010000000
VERSION     DAOQII010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9313_length_5200_cov_3.761322"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /gene="guaA"
                     /locus_tag="PLF48_08755"
     CDS             <1..1520
                     /gene="guaA"
                     /locus_tag="PLF48_08755"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNF49455.1"
                     /translation="FDFGSQYTQLIARRIRELDIYCEIHPYYAHSILEKTTFSGIILS
                     GSPFSVLEENALQFPVKDIVNTYKKPLLAVCYGAQYIAHIHNGKVERSQKREYGRANL
                     NYIESENKLFSYIHRGSQVWMSHGDSILQLPDDFELLASTDAIPVAAYKHKTDEVYGI
                     QFHPEVTHSLDGKQLVKNFAIDICHCSQDWTSGAFADETIVEIKDTIGTDHVVLGLSG
                     GVDSSVAALLLHKAIGSQLTCIFVNNGLLRLNEFDNVLDSYKHLGLNVIGVDASEKFY
                     AELVGKTDPEAKRKAIGKVFIDVFQEEAEKLDNVKWLGQGTIYPDVIESVSVHGPSAK
                     IKSHHNVGGLPEKMHLKIIEPLKKLFKDEVRKVGAYLGLPEEILHRHPFPGPGLSIRI
                     LSEITPEKVLMLQKADAIFIDGLKKENLYDSVWQAGVMLLPVQSVGVMGDERTYENVV
                     ALRAVTSVDGMTADWCHLPYEFLARISNEIINNVKGINRVVYDISSKPPATIEWE"
     gene            1540..2832
                     /locus_tag="PLF48_08760"
     CDS             1540..2832
                     /locus_tag="PLF48_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF49456.1"
                     /translation="MLFALNACAAKKITTTKNAEIVAIEKPSPSAKESGDSITVKPVI
                     FDIKDNTVKETKPIVAEVSAVNDPYKKYNIAILLPFNLEQIPIGKFIVDSTKILSTES
                     TDAIEFYLGAQLAKQRILNNSLTANVYFLDVKNDSASVQKALSSKPFPKVDFIIGPLY
                     SKTLKMVADYALQNHIPMISPLVNSMYIKNNPYYFNANASLKSQYSFLLDEMKKNHPY
                     KTIEVLYDGTDSTAESITILKDLAENNLKVSNIKYTPVKSWDDAAKLLYAADTLSERM
                     VLIYSSKEPYIKSLLGKLKPLKNKLNIYTSSSLRYSKGLADLKLTDNVFTAYPYNSDN
                     INFRQFTDRYTLKYVKKPDETAYQAYDILMHLLYMIDKHQTLTDNNYINTLDFDNTQN
                     KFNFKPIINKENSVDYYDNTHLNLYKLVNGTFLLNPVE"
     gene            2883..5132
                     /locus_tag="PLF48_08765"
     CDS             2883..5132
                     /locus_tag="PLF48_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HNF49457.1"
                     /translation="MHKIYTFLLLTYLGITLVDAQYNVQLLARKTYSGQNLSGSWGYV
                     DPVTQKEYGLIGTSKGLSIVDVSIPTSPVEVKFIPGSNGLWRECQTYRSFAYITQDNN
                     GNTNSEGILIYDLSTLPAGKVDTFRGTTINDSIFRTHSLYIDTIQGFLYLNGGRFKVG
                     NSGQNGVAIYDLKPNPKKPVFVGYTPSAAGTSLNYVHDCYVRDNIMYQAHVYNNRFTV
                     WDVTNKANPIKLQDFTTAYSTVHNMWLSKDSKTLFVTHEVYNYPVEAFDVSNLNNIRK
                     LSEFKVNPTNQEIAHNVHVIDENFAYTSYYSDGLAIFDISDPNNVITVGYYDTQPGST
                     RTENGVWGAYGYYHSGIFTLSDMIAGMFVVKPTLVRAARIVGTVTDTNTNAAIIAATM
                     TIVDTSITATTDFSGKYRTGTAKAGSTTLKFEKTGYITKYVTLTLTNGKIDTVNVKLR
                     QIPFVKNTVNTQFCEGNSYLLPDGRAVSLPGVYESVVATSSSGLDTVIITNLTQNPSY
                     QFTQSAEICQGENFTLPWGGTANTSGLYQHNYQTTKGCDSVLAINLTVNPAYNIEFNP
                     VICIGESYNLPWGGTAATQGTYQHNYAAVSGCDSVVKVYLSVSPVYDITKFDTILQGE
                     TFTLPDGNQTSIAGTYQSNLLTTSSCDSVIHTVLFVKDTSTVTAIKANFQKFNFYYSS
                     QQNAVIIQPSTTIAIAQLEIFNAVGELIYKNEQAESNYVLPDLAKGLYLVKVLSKNMD
                     LYSGKFIKQ"
BASE COUNT         1716 a          950 c          960 g         1574 t
ORIGIN      
        1 tttttgattt cggttcccag tatacccaac taattgcccg tcgaatccgc gaactggata
       61 tctactgcga aattcatcct tattatgcgc atagtatatt agaaaaaacc acattcagcg
      121 gtattatttt atccggctct ccattttctg ttttagagga aaatgcactt cagtttcctg
      181 tgaaagatat tgtcaatacc tataaaaaac cgctcttagc tgtttgctat ggtgcgcaat
      241 atattgccca tattcataac ggcaaagttg agcgttctca aaagcgggaa tatggcagag
      301 caaatcttaa ttatatcgaa tcagaaaata aacttttttc ttacatacac agaggcagtc
      361 aggtttggat gagccatggc gattctattt tgcagttgcc cgatgatttt gaacttttag
      421 ccagcacgga tgccatcccg gtcgccgctt ataaacataa aacagatgag gtttatggca
      481 ttcaattcca tccggaagtt actcattctt tagacggaaa acaactcgta aaaaattttg
      541 caatagatat ctgtcattgt tcgcaagact ggacttcggg tgcatttgct gatgaaacca
      601 ttgttgaaat caaagacact attggaacag accatgttgt actcggttta tcaggcgggg
      661 tagattcttc tgtagctgcg ttattattgc ataaagccat tggcagtcag ctcacttgca
      721 tttttgtcaa taacggtttg ttgcgtttaa atgaatttga taacgtgctc gattcttaca
      781 aacatttagg actgaacgtg attggcgtgg atgcttcaga aaaattttat gctgaattag
      841 taggtaagac agatcctgaa gcaaaacgaa aagccattgg caaggttttt atagatgtat
      901 ttcaggaaga agctgaaaag ctggacaatg taaaatggct ggggcaagga acaatctatc
      961 ccgatgttat cgagtcggtt tctgtacacg gtccgtctgc aaaaataaaa tcacatcaca
     1021 acgtcggtgg gctaccggaa aaaatgcacc tcaaaataat agagccactt aaaaaattat
     1081 ttaaggatga agtgcggaaa gtaggtgcgt atctgggttt acccgaagaa attttacatc
     1141 gccatccgtt tccggggccc ggtttgagca tacgtattct cagcgaaatt acacctgaaa
     1201 aagtgctgat gctgcaaaaa gctgatgcaa tattcattga tggcttaaaa aaagaaaatt
     1261 tatatgacag tgtatggcaa gccggcgtca tgttgcttcc ggtgcaaagt gtgggtgtaa
     1321 tgggtgatga aagaacttac gaaaatgttg tagctttgag ggcagttacg tcggtggacg
     1381 gcatgacggc agactggtgt catttgccct acgagttttt ggcacgaatt tcgaatgaaa
     1441 taatcaataa cgtaaaagga ataaacagag ttgtctatga tatcagttca aaaccacctg
     1501 caactattga gtgggaataa gatatggatg ttacttttta tgctgtttgc attaaatgct
     1561 tgcgctgcaa agaaaattac aaccactaaa aatgcagaaa ttgtagccat tgagaaacct
     1621 tctccatctg ctaaggaaag cggtgattct attactgtaa aacctgtcat ttttgacata
     1681 aaagataaca cagtgaaaga aacaaagcca attgtggctg aggtttcggc ggtaaatgat
     1741 ccgtataaaa aatacaatat tgccatactt ttgccattta atctggaaca aattcctatt
     1801 ggtaaattta ttgtggactc tacaaaaata ctgagtacag aatctacaga tgccattgag
     1861 ttttatctgg gagcacaact ggcaaaacag cgcattttaa acaattcgct tacggcaaac
     1921 gtgtattttc tggatgtaaa aaacgattcg gcttcggtgc aaaaagcgtt gtcctccaag
     1981 ccatttccaa aagtagattt tatcattggt ccactttatt caaaaacgct aaaaatggta
     2041 gctgactatg ctttgcaaaa tcatattcct atgatttctc cattagtcaa ttctatgtat
     2101 atcaaaaaca atccttatta ttttaatgca aatgcctcat tgaaaagcca gtacagtttt
     2161 ttattggacg aaatgaaaaa aaatcatcca tataaaacca tagaagtgct ttacgatggt
     2221 acagattcga cagctgaaag tattactatt ttgaaagacc tagcagaaaa taaccttaaa
     2281 gtatccaaca ttaaatacac gccggtaaaa agctgggatg atgctgccaa actattatat
     2341 gctgcagata ctttgagtga gcgcatggta ttgatttatt catctaaaga gccttatata
     2401 aaaagtctgt tgggcaaatt aaaaccgcta aaaaacaaac tcaatatcta tacttcgtca
     2461 agtttgcgat attccaaggg tctggctgat ttaaaactca cggataatgt gtttacagcc
     2521 tatccatata attcagacaa tatcaatttc agacaattta cagacagata taccttgaaa
     2581 tatgtaaaaa aaccggatga aacggcatat caggcttatg atatactgat gcatctgctt
     2641 tatatgatcg acaagcatca aaccttaacc gataataatt atatcaacac gcttgatttt
     2701 gacaacacgc agaacaaatt caactttaag cccattatta ataaagaaaa cagcgtagat
     2761 tattacgaca acacccatct caatttatac aaattggtaa acggtacttt tttgctcaat
     2821 ccggtggaat aagagtattg atgtaagaaa aaattgtatt atttttatgc attactaaat
     2881 ttatgcataa aatatacact tttcttttac tgacttactt aggtattaca ttagtagatg
     2941 cgcaatataa tgtgcaatta ctcgccagaa aaacttattc gggtcaaaat ctatccggtt
     3001 cttggggata tgtagatcct gttactcaaa aagaatacgg actaataggt acgtcgaaag
     3061 gactttctat tgtggatgta agcataccga cctctccggt agaagtcaaa tttattccgg
     3121 gttctaatgg cttatggcgt gagtgtcaga cttacagatc atttgcctac attactcaag
     3181 acaataatgg aaatactaat agcgaaggta tattgatata cgatttatct actcttccag
     3241 caggaaaagt ggatactttc agaggtacta cgataaatga ttcaattttc aggacacatt
     3301 ctttatatat agataccata cagggatttt tatacttgaa tggtggcaga tttaaagtgg
     3361 gaaattccgg tcaaaatggt gtggctattt atgacttaaa acccaaccct aaaaaaccgg
     3421 tatttgtcgg atatactcct tccgcagcag gcacctcact taattacgta catgattgtt
     3481 atgtgagaga caatatcatg tatcaagcac atgtctacaa taacagattt accgtttggg
     3541 atgtaactaa taaagctaac ccaataaaat tgcaagattt tacaactgcc tactcaacag
     3601 tacataatat gtggttatca aaagacagta aaacactttt tgttactcat gaggtttata
     3661 attatccggt ggaagctttt gatgtcagta atttaaacaa tatccgcaaa ttatcggaat
     3721 ttaaggttaa cccgactaat caggaaattg cacacaatgt acacgtaatt gatgaaaact
     3781 ttgcgtatac atcatactat tcagatggtt tagcaatttt tgatatttca gaccccaata
     3841 atgtaattac cgttggttat tatgatacac aaccaggttc aaccagaaca gagaatggtg
     3901 tgtggggcgc ttatgggtat tatcactctg gcatttttac acttagtgac atgatagctg
     3961 gtatgtttgt cgtaaaacct actttggttc gagctgcccg tatagtagga actgtaaccg
     4021 atacgaatac caatgccgcc ataatagctg ctactatgac aatcgtggac acgagtatta
     4081 ccgctactac tgatttttcc ggaaaataca gaaccggaac agcaaaagca ggctctacta
     4141 ctctgaaatt tgaaaaaaca ggctacatca ctaaatatgt aacactcacc ttgactaacg
     4201 gaaaaattga caccgtaaat gtaaaattaa gacaaatccc atttgttaaa aacacggtca
     4261 atacacaatt ttgtgaaggc aacagctacc tacttccgga tggacgtgcc gtttcacttc
     4321 cgggtgttta cgaatctgta gtggctacaa gttcaagcgg tcttgatact gtcattatca
     4381 ccaatcttac acaaaatcca tcttatcaat ttacgcaatc agctgaaatt tgtcaaggag
     4441 aaaattttac acttccttgg ggtggaacag caaatacatc aggcttatat caacacaact
     4501 accaaactac taaaggatgt gattctgttt tagcaattaa tcttacagta aatcctgctt
     4561 acaatatcga atttaatcca gtgatttgta ttggcgaaag ttataatttg ccttggggtg
     4621 gcactgctgc aacacagggt acctatcaac acaattacgc cgctgtgtcc ggttgcgatt
     4681 ctgttgttaa agtatatctg tcggtttctc cggtttatga catcaccaaa tttgacacca
     4741 ttttacaagg tgaaacattc accctgcctg atggaaatca aacaagcata gcgggtactt
     4801 atcaaagtaa tttactgact acttcatctt gcgactctgt tattcatact gtgttatttg
     4861 taaaagatac gtccactgtt acagcaatca aagctaactt ccagaagttt aatttttatt
     4921 attcttctca acaaaatgct gttataattc aaccatcaac tactattgca atagctcaac
     4981 ttgagatttt taatgccgta ggagaattaa tttacaaaaa tgagcaagct gaaagcaatt
     5041 atgttttacc tgatttagca aaaggcttat atcttgtaaa agtgctttca aaaaacatgg
     5101 atttgtattc ggggaaattt attaagcaat agaatttaat aacatactac agctaaagtg
     5161 ggataaattt ttccgctgat taagtttata tctcattttt
//
LOCUS       DAOQII010000183         5160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9415_length_5160_cov_5.141234, whole genome shotgun sequence.
ACCESSION   DAOQII010000183 DAOQII010000000
VERSION     DAOQII010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9415_length_5160_cov_5.141234"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLF48_08770"
     CDS             complement(<1..654)
                     /locus_tag="PLF48_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49458.1"
                     /translation="MKKLNLKLASKSLILLIGMSIFFVGCKKDKTTPDTNNYGSIKTD
                     NWRSNIGTEYTVEGYYDEENGIGKIYANTKDRLMDGLMQQENYILIEKPDAITYVPDF
                     RPYIGKKIKITGKLKAAIEPSLVNSAQMSGIGDKSLATIKTSLVSVSDSVSLFAPATR
                     LSFCSLYPILCSGIQFNYNKVAFLYSGGIDASNAHTRYYNDIKVLYWLLKNKYGFSDA
                     "
     gene            1005..2081
                     /gene="gcvT"
                     /locus_tag="PLF48_08775"
     CDS             1005..2081
                     /gene="gcvT"
                     /locus_tag="PLF48_08775"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNF49459.1"
                     /translation="MKTTPLTAIHESLGAKMAEFAGFYMPIRYTSDIEEHNCVRQKVG
                     VFDVSHMGEFVIKGEKALPFLQKIISNDVAKLERGQAQYAYLPNKNGGIVDDLLVYHL
                     DDGNYLCVVNAGNIDKDWAWFNENNTDGAELHNISDDTALLAIQGPKAEEILQKLTSV
                     DLSAIPYYRFARGNFAGVDNVIISNTGYTGAGGFELYFHKSHAVDIWNKIFESGKDAG
                     IQPIGLGARDTLRLEKGYCLYGNDINDTTSPLEAGLGWVTKFNKDFIAKEILLEQKQN
                     GLKHRLVGFVMIDRGIPRHGYLIKDEHGNQIGEVSSGTQSPSLGYGIGMGYVDIIYSA
                     QDAVIYIDIRGKLLKAKVIKLPFL"
     gene            2083..3297
                     /locus_tag="PLF48_08780"
     CDS             2083..3297
                     /locus_tag="PLF48_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNF49460.1"
                     /translation="MVRWFLLSISLLLIFSCKKGSEIQQSLFTSSTLESYRFVQQLSA
                     QQISNLIDTTGASTGIACIYPVNIYEVTYRTTNYKDEACLAKGIICFPVISRMLIPVI
                     SLQHGAIFSKYNIKADTTEDIFNMAALLCSESGAAVCVPYYIGIGDVTATHHFFNAKE
                     ETNAVIDITRTLTDLSAKTNSAVTIMNELILAGYSQGAHASLATLNKIERLYSRDFNV
                     KLTILISGIYSFKASQQLSYITDSAAYTYTSVFPYLIRSIQETKQLYPYYSSYLKHPY
                     DSLCSSVFSSHLPEDTILTRFPLYATNMLQPDFRNELQTNPQHPFWNALFENDLLNNP
                     NPITPIYFYYSAADEVAKPQNSTLAYNQFRTTNNNVYIENVGNVSHLTSCFAGLKRMR
                     RWVYKQVKLIPY"
     gene            complement(3284..4990)
                     /locus_tag="PLF48_08785"
     CDS             complement(3284..4990)
                     /locus_tag="PLF48_08785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNF49461.1"
                     /translation="MEIFTRKTRQFVNDDFVLKNWQDIESYYTELSNRALHNTDDILA
                     WLSDRSETDSIIDEEYRWRYIRQTCDTENEQYKHEYEDFIQHVMPNWMLLTNDLNKKL
                     ANNPHTEQLDKDRFFIYLRGLKKQIEIFREENIPLTQQIQLLSQEYGATIGAMTIEHN
                     GQEYTMPQASVLLQNQDRKLRKDIFDKIAGRRLKDKQKLNDLFDELLKLRHQMALNAG
                     YANFRDYMFDALGRFDYGVKESEQFHQAVLEEVTPMLNSFYDERKQALDISSLQPYDL
                     EVDIHNEAPLKPFATEKELIQKSVQCLHETDAYFAECIEVMNNMKHLDLESRKGKAPG
                     GYNMTLPELGVPFIFMNAAGTQSDVETIVHEAGHAVHSFLTRDLPYNFDQEITSENAE
                     LASMSMELMSFDGLSYFYDEHNKKRAIRNHLKRIIGIMPWIALVDKFQHWIYLHPGQT
                     AEERESKWQEMHGQFYGNVLDWKDYDELQKIFWHKQLHIFEVPFYYIEYGIAQLGAIG
                     VWRNYKQDKQKAIQQYKEALALGYTKTLPEIYRTAGVAFHFDRAYVKELMQFIMDEIS
                     KV"
BASE COUNT         1541 a          998 c          924 g         1697 t
ORIGIN      
        1 agcatcagag aagccgtatt tatttttcaa aagccagtac aatactttaa tatcattata
       61 gtaacgagta tgagcatttg aagcatctat tccgccactg tataaaaatg caaccttatt
      121 atagttgaac tggataccac tgcacaatat gggatataag ctgcaaaatg aaagacgtgt
      181 tgccggtgca aacaagctca cactatcact tactgaaact aaagacgttt taattgttgc
      241 caatgattta tcgccaatac cagacatttg tgcgctgttt accagactag gctcaatagc
      301 ggctttcaac ttgcctgtaa ttttaatttt tttaccgata tagggtctga aatcaggcac
      361 gtaggtgatt gcatccggtt tctcaatcag gatataattt tcctgctgca tcagcccgtc
      421 catcagcctg tctttcgtgt tggcgtatat ttttcctata ccgttttctt cgtcgtaata
      481 accctctacc gtatactcag taccaatgtt tgaacgccag ttatcggttt ttatactgcc
      541 gtaattgttc gtgtcgggtg tggttttatc ttttttacat ccgacaaaaa agatactcat
      601 tcctataagc aggataagag atttgcttgc tagttttaaa tttagttttt tcattagaat
      661 ttattttgat gtaaaactaa atctgttctc tattatgatt tattttaatg agtattcggt
      721 aacttttaat tagaattcgt taaaattagt cgttaaatta agtatgactt aatgtcgaat
      781 attcaaaata gttgatttta ttgttttaga accacctcgt tttatcccga actcaggtta
      841 ctaaagtcaa cgtagctcca taggcaagcg agattgattc ttaatcaata gtcatgcgta
      901 tgctatgccg gtcattagca ctaatttatc ttgtttttat cgttattttg atatcgaaag
      961 agtaatgact tttcaggcga ttaaatattc cttactttta cagtatgaaa actactccgc
     1021 ttacagcaat acacgaatca ctcggtgcta aaatggctga atttgcaggc ttctatatgc
     1081 ctatccgcta tacctctgat attgaagaac acaattgtgt acgacaaaaa gtgggtgtat
     1141 ttgatgtatc tcacatgggc gaatttgtga taaaaggaga aaaagcactt ccttttttac
     1201 aaaaaataat ttctaatgat gtagccaaat tagaacgtgg tcaggcacaa tatgcttact
     1261 tgcctaataa aaatggggga attgtagatg atttattggt ttatcattta gacgatggca
     1321 attatctttg tgtagtcaat gcaggaaata tagataaaga ctgggcatgg tttaatgaaa
     1381 acaataccga tggggcagaa ttgcacaata tttctgacga tacagctttg ctggctattc
     1441 aaggacctaa agctgaagag atattacaaa aacttacgtc tgtagatttg tctgcaattc
     1501 catattatcg ttttgcccga ggcaattttg ccggagttga caatgtcatc atttccaata
     1561 ccggctatac cggagcaggt ggatttgaac tatactttca taaatcacat gccgttgata
     1621 tttggaataa aatttttgaa tctggcaaag atgccggcat acagcctatc gggttgggtg
     1681 cacgcgatac cctaagactg gaaaaagggt attgtttata tggaaatgat attaacgata
     1741 caacttcgcc attagaagcc ggtttaggct gggttacaaa attcaacaag gattttattg
     1801 ccaaagaaat tctattggaa caaaaacaaa acgggctaaa acatagatta gtcggctttg
     1861 taatgataga cagaggcatt ccccgccatg gttatttgat aaaagatgaa catggaaatc
     1921 agattggcga agtaagctcg ggcactcagt ctccaagttt aggatatggg atcggtatgg
     1981 gatatgttga tattatttat tctgcacaag atgctgtcat ttatattgat atccggggaa
     2041 aattgcttaa agcaaaggtg ataaaactgc cttttttata agatggttcg ctggttttta
     2101 ctttcgattt cgctactact catctttagc tgtaagaaag ggtctgaaat tcagcagagt
     2161 ttatttacct cttcaacttt agaaagctac cgctttgtac aacaattatc cgctcagcag
     2221 atttccaacc tgattgatac cacgggtgcg tctaccggta ttgcttgcat atatccggta
     2281 aacatttatg aagttaccta tcgtactacc aactataaag atgaagcatg cctggcaaaa
     2341 ggaataattt gctttccggt aatcagcaga atgctgatac ctgtaatttc tttgcagcat
     2401 ggtgccatat tttctaaata caacataaaa gccgatacta cagaagatat attcaatatg
     2461 gctgcattat tatgttctga atcaggcgca gcagtctgtg tgccttatta cattggtatt
     2521 ggcgatgtaa ctgcaacaca tcactttttc aatgcaaaag aagaaacaaa tgctgtcatt
     2581 gatattactc gtacactcac cgatttatct gctaagacga actccgcagt aactatcatg
     2641 aatgagctta ttttagccgg ctattcacag ggggctcatg ccagtttagc tactttaaac
     2701 aagatagaaa gattgtatag ccgcgatttt aatgtgaagc taactatcct gatttccggg
     2761 atatactcct ttaaggccag tcagcagctg tcatatatta cagattctgc ggcttacact
     2821 tatacatcgg tttttccata tttaataaga agcattcagg aaacgaaaca gctttacccc
     2881 tattattcat cttaccttaa acatccttac gactcgttgt gttcatctgt tttttcctca
     2941 cacctgcccg aagatactat cttaacacga tttccacttt atgcaacaaa catgctgcaa
     3001 cccgatttca gaaatgagct tcagacgaat cctcaacatc ctttttggaa tgcattattt
     3061 gaaaacgatt tactcaataa tccaaaccct ataacgccga tttattttta ttacagcgct
     3121 gctgatgaag ttgctaaacc tcaaaattcg acactcgcct ataatcagtt cagaacgaca
     3181 aataacaatg tctatattga aaatgtagga aatgtttcac atttgacttc ttgctttgca
     3241 ggattaaaaa gaatgcgccg atgggtttac aaacaggtaa aacttatacc ttactgattt
     3301 catccataat aaactgcatc agttctttta cataagcacg atcaaaatga aaggcaaccc
     3361 cagctgtacg ataaatttcg ggcaaggtct tcgtataacc taaagctaag gcctctttat
     3421 attgctgaat ggctttttgt ttatcttgtt tatagttgcg ccagacgcca attgctccta
     3481 attgtgctat gccatattcg atgtaataaa aaggcacctc aaagatatgc aattgtttat
     3541 gccaaaatat tttttgcagt tcatcatagt ccttccagtc gagtacattg ccataaaact
     3601 gaccatgcat ttcttgccat ttactctcac gttcctcagc cgtttgcccg ggatgtaaat
     3661 aaatccagtg ctgaaactta tctactaagg caatccaggg catgatgcca atgatacgct
     3721 tcagatgatt gcgaatagcc ctttttttat tatgttcatc gtaaaaatac gataatccgt
     3781 cgaaactcat caattccata ctcatcgaag ccaattccgc attttctgag gtgatctcct
     3841 gatcaaaatt atagggtaaa tcacgggtta aaaaagagtg cactgcatgt ccggcttcat
     3901 gaacaatggt ttctacatca ctttgtgtac ctgcagcatt cataaaaatg aatggcacac
     3961 ccaattccgg taacgtcata ttataaccac cgggagcctt gcctttgcgc gattccaaat
     4021 cgagatgttt catgttgttc atcacctcta tgcattctgc aaaataggca tctgtttcat
     4081 gcagacattg tactgatttc tgaattagtt ctttctctgt tgcaaatggt tttagcggag
     4141 cttcattatg aatatccact tctaaatcgt agggttgtaa ggaagagata tccagagctt
     4201 gtttgcgttc gtcataaaat gaattcagca tcggagtaac ctcttccaat actgcctgat
     4261 gaaactgttc gctttctttt actccataat caaatctgcc caaagcatca aacatataat
     4321 ctctgaaatt agcatatcct gcatttaatg ccatttgatg gcgcaatttc aacaactcgt
     4381 caaacaagtc attgagtttt tgtttatcct ttagtcgccg cccggcaatc ttgtcgaaga
     4441 tatctttgcg aagtttcctg tcttgatttt gaagcaaaac ggaggcttga ggcatggtat
     4501 attcctgccc gttatgttct atggtcatcg ctccgatagt ggcaccatat tcttgtgaaa
     4561 ggagttgtat ctgttgtgta agcggaatat tctcttctct gaaaatctcg atttgttttt
     4621 ttaaccctct caggtaaata aaaaatctgt ctttatcaag ttgctccgta tgcggattgt
     4681 ttgccagttt cttattcaaa tcattcgtca acaacatcca gttgggcatg acatgctgaa
     4741 tgaaatcttc atactcatgt ttgtattgct cattttcggt gtcgcaggtt tggcgaatat
     4801 accgccatcg atattcttca tcaataatgg aatctgtttc acttcggtct gatagccaag
     4861 ctaagatatc atctgtattg tgcaacgctc tgttgctgag ttctgtataa tacgattcaa
     4921 tatcttgcca gttctttaat acaaaatcat cattaacaaa ttggcgtgtt tttcttgtaa
     4981 atatttccat aaagcgattt taaaagtgac agaaaattaa agcaaacgaa tataacttac
     5041 tgtagtaagc tatattatat tctacgataa acttctaagc aaatgtacaa ttttttttca
     5101 gcgtattttt tgatataaat acaagtattt gcctaaatgg ctataaagca taagccgttt
//
LOCUS       DAOQII010000184         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9589_length_5099_cov_4.383624, whole genome shotgun sequence.
ACCESSION   DAOQII010000184 DAOQII010000000
VERSION     DAOQII010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9589_length_5099_cov_4.383624"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(409..1047)
                     /locus_tag="PLF48_08790"
     CDS             complement(409..1047)
                     /locus_tag="PLF48_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49462.1"
                     /translation="MAKKKVLITLKTYPAISSKYDELVCTAGFLEDGTWIRIYPIQFR
                     KKSFEEQYKKYDWIKIDLVKNTSDFRKESYRPVSYDTEIKILDHLDTKSNWLLRKEVV
                     YKNKVYTNITELITEAKNKEIMTSLAVFKPTEIIDFTIEQVEREWDKKKLEKLKKDRS
                     SNLFAQENEDLFEAVQKLPYKFSYILLDCNGTKSKMMIEDWEIGQLYWNCLA"
     gene            complement(1050..1934)
                     /locus_tag="PLF48_08795"
     CDS             complement(1050..1934)
                     /locus_tag="PLF48_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNF49463.1"
                     /translation="MFYRRKIILALLQLFDRQIDKISLQKLLFLFTKIQKKAEYDFIP
                     YRYGCYSYSANADLIAMVKRGILSETTSHFINNETTNYFNALKSDDKKLMLYVKDKYG
                     KLNADALMKHTYLNFPYTAINSVRAKEILTAEQFEKVNNSRPKSNKTILFTIGYEGIS
                     LEEYLNRLLKNDVKVLVDVRNNPLSMKYGFSKSQLQRYCISLGIEYVHYPEVGIKSEQ
                     RQELKSQADYDKLFKVYRDNNLTKTTSSQEKILNLLKENKRIALTCFEANICQCHRKH
                     LAEAIENLPNFNYEVKHI"
     gene            2340..5018
                     /gene="alaS"
                     /locus_tag="PLF48_08800"
     CDS             2340..5018
                     /gene="alaS"
                     /locus_tag="PLF48_08800"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNF49464.1"
                     /translation="MKNTLSANDIRQAFLDFFKSKGHLIVPSAPIVVKNDPTLMFINS
                     GMAPFKDYFLGIGKPKSARIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFG
                     DPANPSAGYFKKEAVAWSWEFLTEVLKLDKDRLYVTIFEGNAEENIPRDEEAYNEWKK
                     WIAEDRILLGNKKDNFWEMGDTGPCGPCTEIHVDCRTDEERANTDGKTLVNNDHPQVI
                     EIWNNVFIQFNRKKDTSLELLPAKHVDTGMGFERLVRVIQGKTSNYDTDVFSGTIAHI
                     EKLVQIQYDRSDSKQAIAFRVLADHIRAVAFTIADGQLPSNTAAGYVIRRILRRAVRY
                     YFSYLNRKEPLLHLLVPVLATQFENVFPELKAQQDFVAKVILEEENSFLRTLENGLKR
                     IDIILKETSGKIVSGKSAFELLDTYGFPIDLTNLIATENGFTVNETEFDEELQKQKER
                     SRAATELNVDDWIILAEGESKFIGYDSLSVKSKVLKYRKIKNKDIEQYQIVLELTPFY
                     AESGGQVGDVGVLEFVNPNNPTDITLLPITDTKKENNLVVHFSDAIPANLNGEVFAKV
                     NPAIRKKTTIHHSVTHLMHAALRHVLGTHVAQKGSLNNSEYLRFDFSHFQKMSDEEIA
                     RVEQIVNVKIREDIPVVIKEMPKEDALKLGAMALFGEKYGDTVRVVVMDENYSVELCG
                     GTHVGRTGELGLFKITGESAVAAGVRRVEAVCGEAAFEYVQAKINELKTIHQELKNPK
                     DSIKAIQSLMDNNTALKKEIEKYQGEKVESIKQSLRTKVRNQNDVNVLIEKIDGVNAE
                     GLKKIQFDLKAEFDDFYFLAVTVINDKPLISLIIADELIKSKNLHAGNTVKELAKAIR
                     GGGGGQAFYATAGGTDSSGVDTVLKQAEELFLVQTS"
BASE COUNT         1610 a          837 c          992 g         1660 t
ORIGIN      
        1 ctttatctgt ccaggattta aaaacttgtt caaattcaaa atctgttttt tcagggatga
       61 gcaggacagt tttgttcatt atttggtctt aatatgagtg aattaatacg aatagttata
      121 tagcttgtta atataataaa ataaatatta aaaataaatg gtatatagtg aagttgtgaa
      181 atggatatgg atgttattgg tattacctct tgtcatttgc tatcaaaaaa atgatgtttg
      241 tttgtctatt tttgggtgaa aagtgccgat aatcataaac ggatttttgc ttgttagatg
      301 atgaagttga gatgtgccta gaaataaatg taagtctttt gtttttgcaa aatcatcaaa
      361 atatttcttc ttaacatctg caactgcatt agactcgttg ccctcatgtc atgcaaggca
      421 attccaataa agttgtccta tttcccaatc ttcaatcatc attttacttt tcgttccatt
      481 acagtcgagt aaaatataag aaaatttgta cggaagtttt tgtactgctt caaataaatc
      541 ttcattttct tgggcaaata aattgctact tctgtctttt tttaactttt caagtttctt
      601 tttatcccat tctctctcca cttgttcaat agtaaagtca attatctcag ttggtttaaa
      661 tacagccaat gaagtcataa tttctttgtt cttggcttct gtaataagtt cggtaatgtt
      721 tgtataaact ttatttttgt acacaacttc ttttcttagt aaccaattgc ttttagtgtc
      781 taagtggtcc aggatcttta tttccgtatc gtaagaaaca ggtcggtagc tttcttttct
      841 gaaatcactt gtgtttttca caaggtcaat ttttatccaa tcgtattttt tgtattgttc
      901 ttcaaaagat tttttgcgaa attgaatagg ataaattcta atccaagttc cgtcttcaag
      961 aaatcctgct gtgcaaacca attcatcata tttacttgaa atggctggat aggtttttag
     1021 agtgattaaa acttttttct ttgccatttt taaatatgct ttacttcata attaaaatta
     1081 ggcaaattct caattgcttc agctaaatgt ttacggtggc attggcaaat gttcgcttca
     1141 aaacaagtta aggcaatacg tttgttttct tttagcaagt ttaaaatttt ctcttgagag
     1201 gatgttgttt ttgtcaaatt attatcacga taaactttaa ataatttgtc gtaatctgct
     1261 tgtgatttta attcttgtcg ttgttcagat tttattccaa cttcgggata atgtacatat
     1321 tcaataccca aactaatgca atagcgttgt agttgacttt tgctaaaccc atatttcata
     1381 cttaagggat tgttgcgaac atctaccaaa acttttacat catttttcag caaacgattt
     1441 aagtattctt ccaatgaaat gccctcgtag ccaatggtaa acaaaattgt tttattgctt
     1501 ttggggcgac tgttatttac tttttcaaat tgctcagccg ttaaaatttc ttttgctctt
     1561 acgctgttta tggctgtata gggaaagttt aaataagtgt gcttcatcaa agcatctgcg
     1621 ttcaatttac cgtatttgtc tttcacatac aacatcaact ttttatcatc ggattttaat
     1681 gcattaaaat aattcgttgt ttcattgttt ataaaatgac ttgttgtttc gcttagtata
     1741 cctcttttga ccatcgctat caaatcggca tttgcggaat atgaataaca tccgtatcga
     1801 tagggtataa aatcatattc agcctttttc tgtattttag taaacaaaaa cagcaacttt
     1861 tgaagactaa tcttgtcaat ttgtctatca aataattgca gtagtgctaa tataattttc
     1921 cttctgtaaa acattcttca aagttagcaa aatagactag attaactatg ttactttatc
     1981 taatttcaat ataaacataa ggttttgtct gtctgacgag agtgttgaga gagaattgct
     2041 cataacgtag tcactgaaaa aaacaccggt ttgcctattt ttcatttttc taaaggtaat
     2101 tatttgtttc atcaatgaca atgcaaaatg tatgttttta tcaacatatt tatatttgtc
     2161 tattcttttt tttacttttt aaagaggctg actttttact tcttttcatg ttattcttcc
     2221 cttttttatc tccattttac tcaaaaatac ttaaacccaa tgcatgaatt ttgttgctgg
     2281 tgcttgcttc tgtttatggt aacaatacta cttgcaaaag gaattattat cttcgcacca
     2341 tgaaaaatac gctttccgct aacgacatcc gacaagcttt tctcgacttt tttaaaagca
     2401 aaggtcatct aatagtgcct tctgctccca tagtggttaa aaatgatcct acgctcatgt
     2461 ttatcaattc gggaatggca ccgtttaaag attatttttt aggcataggt aaacccaaat
     2521 ctgcccgtat tgccgatacg caaaaatgtc tgcgtgtttc cggaaaacat aatgatttgg
     2581 aagaagtagg agtggacact tatcatcaca cgatgtttga gatgttaggc aactggagct
     2641 ttggagaccc tgcaaatcca agtgctggtt atttcaaaaa agaagctgta gcctggagtt
     2701 gggagttttt gacggaagtg ttgaaattgg ataaagaccg cttgtatgtt acaatttttg
     2761 aaggcaatgc ggaagaaaat atccctcgtg acgaggaagc ctacaacgaa tggaaaaagt
     2821 ggatagcaga agaccgcatt ttactcggca ataagaaaga taatttctgg gaaatgggcg
     2881 ataccggtcc ttgtggtccg tgtaccgaaa tacatgtcga ttgccgaacg gatgaagaac
     2941 gggcaaatac agacggaaaa acattggtga ataatgacca tccgcaagta atagaaatct
     3001 ggaataatgt atttattcag ttcaacagga aaaaagatac ctcattagaa cttttgcccg
     3061 ccaaacatgt agatacaggc atgggctttg aacgcttggt gcgggtgata caaggaaaaa
     3121 cctccaacta cgatacggat gtattttcgg gtacgattgc gcatatagaa aaactggtac
     3181 aaatacaata tgaccgaagc gacagcaaac aagctattgc atttcgcgtg ttggccgatc
     3241 atatcagggc ggtggctttc accattgcag acggtcagtt accaagcaac acggcagcag
     3301 gctatgtgat acgccgtatt ttgcgcagag cagtgcgtta ttatttttct tacttaaaca
     3361 gaaaagaacc gctgttgcat ttgttagttc cagtattggc tacacaattt gaaaatgttt
     3421 ttcctgagtt gaaagcacag caggattttg ttgcgaaagt aattctggaa gaagaaaatt
     3481 cctttctgcg cacactcgaa aacgggctta agcgtattga tatcatactg aaagaaacaa
     3541 gtggaaaaat agtaagcgga aagtcggcat tcgagttgtt ggatacgtat ggtttcccga
     3601 ttgacttaac gaatctgatt gctacggaaa atggttttac cgtaaatgaa accgaatttg
     3661 atgaagaact gcaaaaacaa aaagaacgca gtcgcgcggc aaccgaacta aatgttgatg
     3721 actggataat attagcggaa ggcgagtcta aatttattgg ttatgattca ttaagcgtaa
     3781 aatcaaaggt tttaaaatac cgcaaaataa agaacaaaga tatagagcaa tatcaaatcg
     3841 tattggagct aacaccgttt tatgctgaaa gcggcgggca agtaggggat gtgggcgtat
     3901 tagagttcgt caatcccaac aatcctacgg atattaccct gttgcctatt accgatacga
     3961 aaaaagagaa taatctggtt gtacatttca gtgatgctat tcccgcaaat ttaaacggag
     4021 aggtgtttgc aaaagtaaat cctgctatca gaaagaaaac gaccatacat cactccgtta
     4081 cgcatttgat gcatgccgct ttgcgacacg ttttgggaac tcatgtagcg caaaaaggtt
     4141 cattaaataa tagcgaatac ttacgattcg acttttcaca tttccagaaa atgagcgatg
     4201 aggaaattgc gcgagtagag caaatcgtca acgtaaaaat acgcgaagat attccggtag
     4261 tgataaaaga aatgccgaaa gaagatgccc tgaaactcgg tgcaatggct ttattcggtg
     4321 aaaaatacgg cgacacggtg cgtgtggtgg tcatggatga aaattattct gtggagcttt
     4381 gcggaggtac acatgtaggc agaaccggtg aattgggttt gtttaagatt acgggtgaaa
     4441 gtgctgttgc agcaggtgtt cgtcgggtag aagcagtttg tggggaggct gcatttgaat
     4501 atgtacaggc aaaaataaac gagctgaaaa cgatacatca ggaattaaaa aatccgaaag
     4561 acagcattaa ggctattcaa tcattaatgg ataataatac ggcgcttaag aaagaaatag
     4621 aaaaatatca gggcgagaaa gtagaaagca ttaaacaaag tttgcgtact aaagtgcgca
     4681 accaaaatga tgtaaatgtg ctgatagaaa aaattgacgg tgtgaatgct gaagggctga
     4741 aaaaaattca gtttgattta aaagcagagt tcgatgattt ctatttttta gcggttaccg
     4801 ttataaacga taaacctttg atttcgttaa tcattgcgga tgagttgata aaaagtaaaa
     4861 atcttcacgc cggaaacacg gtaaaagaat tagccaaagc gataagaggc ggcgggggag
     4921 gacaagcatt ttatgccaca gccggtggaa ctgacagcag tggagttgat acagtactca
     4981 aacaagcaga agaattattt ttagtgcaga ccagctgatt gttttaatat agaattggat
     5041 actaattttt agccagttcg agcatcattt cccagatatt ttcaaatggg ataacttcc
//
LOCUS       DAOQII010000185         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9629_length_5086_cov_4.853906, whole genome shotgun sequence.
ACCESSION   DAOQII010000185 DAOQII010000000
VERSION     DAOQII010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9629_length_5086_cov_4.853906"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLF48_08805"
     CDS             <1..494
                     /locus_tag="PLF48_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNF49465.1"
                     /translation="KVYPLQKMLGAICSPFEAFLLNNGIKTLPLRMEQHQENALALAK
                     FLHQHEKVNFVNYIGLEEHKDHQLAKKIMTGYGGMLSFELKGGLESGMNFLRNIRFCT
                     LTASLGTADTLVTHPASTSHVNVSIEQRLTYNITDGLIRISVGMENIEDILDDFNQAL
                     SKV"
     gene            complement(491..1039)
                     /locus_tag="PLF48_08810"
     CDS             complement(491..1039)
                     /locus_tag="PLF48_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49466.1"
                     /translation="MNIRLYSFILIALFAWQTENVHAKPKKKKAPITDTITTELSVQS
                     VQDTVSQKLTEPEAAPPVPGVIIIDNFTEQLYDSTPAPNGQFKLEQHAEKIASVYQLK
                     VDLTDQKMIINAPVFDSLSNFFYVIFSDKNGKILINSKLNREQPKMEINMPALINTDY
                     VLNIVNADFTKSNLYYFKRQKH"
     gene            complement(1052..3031)
                     /locus_tag="PLF48_08815"
     CDS             complement(1052..3031)
                     /locus_tag="PLF48_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49467.1"
                     /translation="MKFIYTILLSYFFLTAVAQPITTAVIPPPNQLRCSDVPVRLNGV
                     VFGAVPPNSQDKPVVVFVHGWFDNGYAWFMSKNQWYNLTYNEGYRSAYFFHHYSDAFE
                     KNGKVIADMIRETCRHFNTSKVIAVCHSKGGLDIDWALYNYGIWDSVQGVVTLSSPYY
                     GAPVADLIANPLIRLVTENIPIVGPIFKGKGTYQMQTAYMMSTVRPMLDNHPNNNPQK
                     FHCFGAWGMEHPTRLPSAISDDILKVVFPQYRPLCIEIPGFGDLAGTLMSGFMYATGF
                     LTKLVPVQERYQNPYHNTIINDGLVPYYSSQRPGSTEISPRPNAQESFLNHIDVFRSS
                     ETWDNVKPELEYFTLHPQLRTAPAEQQNLKPEAVEAVSYVALFQSDIISFEKNENSEF
                     ALVGNYDQVKADIFNESNEVVKSFTIQHQTQFMFDFISPLDLKDLANGHTYTLKTSSP
                     LTLFIKDGSGATLQLNTRSNEDLYKNRPLDFEVELANWNEDLYPVSVEGTLSRNMDEN
                     GEVVYDKLIPVHFKFDPQSQKFICKDAPDLATGIYNVSVYAKGNKTQRFATTSMAIAM
                     KEHTAVNNRNLKIYPNPAAQNLNISFTSDFKNITLQITDVLGKVQYQKLLFADETFMH
                     ELQLDLNELNLASGLYALSIVENEKIAASKTFVKQ"
     gene            complement(3169..4449)
                     /locus_tag="PLF48_08820"
     CDS             complement(3169..4449)
                     /locus_tag="PLF48_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49468.1"
                     /translation="MNYTKKEIYRYWWTIAIMMYIMYINVMHKWGHQGDMTCWMDWAK
                     YMRINGLTKIYDNTETRCDYFPAYLYILWLHGKIQVSMQNLQDNLYTIKWFTLIFDFL
                     GAFLAVWYVREPIKKVALFIFLMLNIAYIYNTVQWAQVDAIFTFFGFAAVIAALEKKV
                     SLSIVSFMLCINFKLQAIVFIPVLGLILLPQFLSKKGVQQLFIGLFFGLLIQLAILFP
                     FIVKGKLDKVWHVFTHLAGQYPYPSLCAFNIWFWFFPNTTIEGMYELNDGVKLGFLTL
                     RQIGNLMFLVMMLATVFPLMKYEYQTLIEKKILSFPLEKIFLMCALVPLCFFYFNTEM
                     HDRYTHPALIALAAYAFCSVNKLRYIPLILGSVAYLLNLEKPLRVMALTNYETLIFDP
                     KFGAVLWGILIMLLFYLLYSRKIDTNEELILHIQ"
     gene            4524..>5086
                     /locus_tag="PLF48_08825"
     CDS             4524..>5086
                     /locus_tag="PLF48_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNF49469.1"
                     /translation="MENSLSEIVRNLPQLPGVYRYYDTERKLLYIGKAKSLKNRVSSY
                     FVNKNHSNRIKQMVRKIAQIEYTVVATEKEALLLENNLIKELQPRYNIMLKDDKSYPY
                     IKITNEAYPRVLFTRQLLEDGAEYFGPYTSVHHVRSITELIKKIYPTRTCTLPLTQKN
                     IERHKFKVCLEYHIGNCLGPCENHQSYV"
BASE COUNT         1668 a          897 c          952 g         1569 t
ORIGIN      
        1 caaaagttta tccactccaa aaaatgttgg gagcaatatg ttctcctttt gaagcttttt
       61 tgttgaataa tggcattaaa acattgccct tgcgcatgga gcagcatcag gaaaatgcat
      121 tggctcttgc gaaattcctt catcaacatg aaaaagtaaa ttttgtgaat tacattggct
      181 tggaagaaca taaagatcat caactggcaa aaaaaataat gacgggatat ggtggtatgc
      241 tgagctttga actaaagggc gggcttgaat ccgggatgaa ttttttaaga aatatccgtt
      301 tttgtacact tactgcttct ttgggtactg cggatacatt ggttacacat cctgcttcaa
      361 cttcgcatgt caatgtaagc atagaacaac gattgacata caatattaca gacggcttaa
      421 tacgcatatc ggttggtatg gagaatattg aagatattct ggacgatttt aatcaggctt
      481 tgagtaaggt ctaatgtttt tgtcgtttga aataatacaa attagactta gtaaaatcag
      541 cattaacgat gttcagcaca tagtccgtat ttattaatgc aggcatattg atttccattt
      601 tgggttgctc acggtttagc ttagagttga ttagtatttt cccattttta tctgaaaaaa
      661 taacatagaa aaaattggat aaagaatcaa aaaccggagc gttaataatc attttttggt
      721 ctgttaaatc aactttgagt tgatataccg atgctatttt ttctgcgtgt tgttccagct
      781 tgaactggcc atttggagca ggtgtactat cataaagttg ttcagtgaaa ttgtcaataa
      841 ttattacacc cggaacaggt ggggcagctt caggttcagt cagtttttga gaaacagtat
      901 cttgtacact ttgaactgaa agttccgtag tgattgtatc tgtaatgggt gcttttttct
      961 ttttgggttt tgcatgcaca ttttcagttt gccatgcaaa tagtgcgatt aagataaagg
     1021 aatatagtcg aatgttcatg gttttatatc tttattgttt tacgaaagtt ttggaagctg
     1081 caattttttc attttcaaca atagataaag catacagtcc tgaagccaag ttgagctcat
     1141 tcaaatcaag ttgcagttcg tgcataaatg tttcatcagc aaacaacagt ttctgatatt
     1201 gcacctttcc caatacgtcc gtaatctgta aggtgatgtt cttgaaatca gaagtgaagc
     1261 tgatgtttaa gttttgggct gccggattag gataaatttt taaatttcga ttattaacgg
     1321 cagtatgttc tttcatggca atagccatac tcgtggtggc aaaacgttgc gttttatttc
     1381 ctttagcata gacagaaaca ttataaatcc cagttgccaa atctggagca tctttgcata
     1441 taaacttttg gctttgcgga tcaaatttaa aatgaaccgg aataagttta tcatatacta
     1501 cttctccgtt ttcatccata tttctgctca atgtgccttc cactgaaaca ggataaaggt
     1561 cttcattcca gttagctaat tctacttcaa aatcaagcgg tctgttttta tacaaatctt
     1621 cattcgagcg ggtatttaat tgaagtgttg cacccgatcc gtctttgatg aaaagcgtaa
     1681 gtggtgatga tgtttttaaa gtataagtat gtccgtttgc taagtctttt aaatccagcg
     1741 gtgaaataaa atcaaacata aattgtgttt gatgttgtat ggtaaatgat tttactactt
     1801 cattactttc attaaaaata tcagctttta cttgatcgta atttcctact aaagcaaatt
     1861 cagaattttc gtttttctca aaagaaatta tgtcgctttg gaataatgca acataagaaa
     1921 cagcttcaac agcttcgggc tttaaattct gctgctctgc aggtgcagtt cgtaattgag
     1981 gatgtagagt gaaatattct aactcaggct tcacattatc ccatgtttct gaggaacgga
     2041 aaacatcaat atggttcaga aatgattcct gcgcattggg tctcggagaa atttcagtac
     2101 tgccaggacg ctgactggaa tagtagggca ccaatccgtc attgattatt gtattgtgat
     2161 agggattttg gtagcgttct tgtaccggta cgagctttgt taaaaatccg gtggcataca
     2221 taaaaccgga catcagtgtg cctgccaaat caccaaatcc gggaatttct atgcatagtg
     2281 gtcgatattg tggaaagact actttcaaaa tatcatctga tatggctgat ggaagccggg
     2341 ttggatgttc catgccccaa gctccaaaac aatgaaactt ttgtgggtta ttattcggat
     2401 gattatccag cattgggcga accgtactca tcatgtaggc ggtttgcatt tggtaagttc
     2461 cttttccttt aaaaattgga cccactatcg gaatattttc agttaccaag cgaattaaag
     2521 gattggctat taaatcggca acgggtgcac cataataagg cgaacttagc gtaacaacac
     2581 cctgtaccga atcccagata ccataattgt ataaggccca atcaatatcc aaaccacctt
     2641 tactgtgaca aacggcaatg actttacttg tattgaaatg gcggcaagtt tcacgaatca
     2701 tgtctgctat aactttgccg ttcttttcaa atgcatcact gtaatgatgg aagaaatagg
     2761 cagaacgata accttcatta taggtcaggt tataccattg atttttggac ataaaccaag
     2821 cataaccatt gtcaaaccag ccatggacga aaacaactac aggtttatct tgcgaatttg
     2881 gaggaacagc accaaacact actccgttta acctaacggg aacatctgaa cagcgcaact
     2941 gattgggtgg aggtataact gccgtagtga taggttgagc tactgccgta aggaaaaaat
     3001 aactcaataa gatggtgtag ataaatttca ttccatgtaa ttaaagatga aaaataagcg
     3061 aaatatttga tttaaacaag cgttgagatt agcgaataat aaatttttcg cacctacaat
     3121 atattgcaaa taaaactaat ttaaggcata atgaataaac tgattaagtt attgaatatg
     3181 cagtatgagt tcttcattag tatcaatttt tcgggaatat aagagataaa aaagcagcat
     3241 aatcagaatc ccccaaagta cggcaccaaa tttcggatca aaaatcaggg tttcataatt
     3301 ggtgagtgcc atcacacgca ggggtttttc aaggtttaat aaataagcca ccgaacctaa
     3361 aattagagga atataacgca atttgtttac actgcaaaat gcataagctg ccagcgcaat
     3421 taaagccgga tgtgtatatc ggtcgtgcat ttctgtatta aaataaaaga aacacaatgg
     3481 cactaaagca cacatcagga atattttttc caatggaaaa gatagaattt tcttctcaat
     3541 caatgtctga tattcatatt tcattaaagg aaagacagtt gcaagcatca tcactaagaa
     3601 cattaaattg ccgatttgtc tcaatgttaa aaatccaagt tttactccat cattcagttc
     3661 gtacatgcct tcgatagttg tattagggaa aaaccaaaac cagatattaa atgcgcacaa
     3721 actcgggtaa gggtattgcc ctgctaaatg tgtaaataca tgccacactt tatctaattt
     3781 tccttttact ataaatggaa acaagatggc aagctgtatg agtaatccga aaaataaacc
     3841 gataaataac tgttgaaccc cttttttaga taagaattga ggcagtaata ttaatcccaa
     3901 tacagggata aaaacgatag cctgtagttt gaaattgata caaagcatga aagaaactat
     3961 acttagactt actttctttt caagagcagc aatgaccgcc gcaaaaccga aaaatgtaaa
     4021 tattgcatcc acctgtgccc actgaactgt gttatagata taggcaatat ttagcataag
     4081 gaagataaat aaagccactt tttttatcgg ttctcttaca taccatacag ccagaaatgc
     4141 acccagaaaa tcaaaaatta aagtgaacca ttttattgtg tataaattat cttgcagatt
     4201 ttgcatgctg acttgtatct tgccatgtag ccataagata tacagatagg caggaaaata
     4261 atcacaacgc gtttcggtat tgtcataaat tttagtcagt ccgttaatgc gcatatattt
     4321 agcccaatcc atccagcaag tcatatcgcc ctgatggccc catttatgca taacattaat
     4381 atacatgatg tacatcatta ttgcaattgt ccaccagtaa cggtatattt ccttttttgt
     4441 ataattcatg tgataaagat aatatcctta tatgtaacta ggataaaata aagtatttaa
     4501 tccagaaaaa ttatctttac aaagtggaaa attcgttaag cgaaatagtt agaaatttgc
     4561 cgcagctgcc gggtgtatac cgctattacg acacggaacg aaaactctta tatatcggca
     4621 aggcaaagag tttgaagaac agagtatctt cttattttgt caataaaaac cactccaaca
     4681 gaataaagca gatggtgcgc aaaatagcgc agatagaata caccgtagtt gccacagaaa
     4741 aagaagcatt actgctcgaa aataatttga ttaaagaact tcagcctcga tacaatatca
     4801 tgctgaagga tgacaaaagc tatccctata taaaaataac gaacgaagct tatccaagag
     4861 tgctgtttac ccgacaactg ctggaagacg gggctgagta tttcggacct tatacctctg
     4921 tacatcatgt gcgttccatc actgagttaa taaagaaaat ctaccccacc cgtacatgta
     4981 ccttgccatt gacgcaaaaa aatattgaac gacataagtt taaagtttgt ttggaatatc
     5041 atattggcaa ttgtctgggg ccatgtgaga accatcagtc gtatgt
//
LOCUS       DAOQII010000186         5079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9641_length_5079_cov_5.137341, whole genome shotgun sequence.
ACCESSION   DAOQII010000186 DAOQII010000000
VERSION     DAOQII010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_5079_cov_5.137341"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLF48_08830"
     CDS             <1..547
                     /locus_tag="PLF48_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49470.1"
                     /translation="NYTDGIKTGKWNYWNKSGQLTTKEESDVLLEESDFYNMRNIDVD
                     FTDYFSAHTHIDILSFPDDIRLYTVRVSPERYPLSSFLPDTIHIKAITAYDHQYMLDQ
                     ATNYHPAENECMLLYELIKPPELNIKPILTFLLGSSVKNKLKSISESSIDDIINIYEY
                     SIINDDYLGFSAMKQALYNAK"
     gene            629..937
                     /locus_tag="PLF48_08835"
     CDS             629..937
                     /locus_tag="PLF48_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49471.1"
                     /translation="MKAFLIKILESHWLHNLLDLSVIGLIVYFFYHSYTSADEIRKLQ
                     ILYLLAILLAVHVLVLLARVAFRIKRKRIVSGFSNSVYLLIQIGFLFAVLFNIHLQRG
                     "
     gene            940..2298
                     /locus_tag="PLF48_08840"
     CDS             940..2298
                     /locus_tag="PLF48_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF49472.1"
                     /translation="MEKQVLHTDVAIIGAGYAGIAAAKLLQANQTDFIVLEARDRIGG
                     RTHSATLASGAQVDLGAQWIGPTQQLIWQWVKETNTETYETYDIGKNILSYKNKTSTY
                     TGTIPKIDPISLLDLGLAIEKINKLCKQIDLEKPWQHPRALEYDSMTFHTWIEKNMHT
                     AKAKHLFHIGVETVFAEEASSLSFLHALFYCRSGENMESLISIKNGAQQTLLKGGTQA
                     LLQKIAQPFSGKIKLNQVVSHIYQSDSGVQIETSDFIVHAKKSILTLPPALLNTIKFS
                     PLLPQRKAQLIQRVPMGAAMKCFVIYKSPFWRKMGYSGQIVSDIHPVRVTFDCTHKDT
                     DLGVLLVFVEGKNARFFLDLTQEERKKQVVSMLVKFFGEDAASYVEYTDKCWTEEEYS
                     RGCYAGNMPPGVLTQFGDELRKPFMHLHFAGTETAYKWNGYMDGAIESGNRATEEVLK
                     LI"
     gene            complement(2356..2985)
                     /locus_tag="PLF48_08845"
     CDS             complement(2356..2985)
                     /locus_tag="PLF48_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNF49473.1"
                     /translation="MGIFGSKEEKTDKFIMQLIEQTQKTNEAIVFLEHSLDEEDIASR
                     LEHLKTITKQIEEIRRVMIDDLLNTFITPIDREDIYNISNALFDMVDYAQTTTDEMRI
                     LNVRADEYIRKMVVLVRRQTEELLMAMERLSKNPRVAHEHALKTKKLEKDVEKQYRKA
                     LRDLFIHPVTPENLPQFFYKREVYRHLSNMADRADTAANIFGIVIMKLT"
     gene            complement(2988..3980)
                     /locus_tag="PLF48_08850"
     CDS             complement(2988..3980)
                     /locus_tag="PLF48_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNF49474.1"
                     /translation="MTPEIWLIILLVLSLTFDFLNGFNDSANVVATIISSRAMSARAA
                     LTLAAVANLIGPFIFGVAVAKTIGHNVADTSSINIYVVIAALMSASMWNVTTWYFGIP
                     SSSSHALIGGIIGAVIMDSGFGAIKLKGIYVILIALFTSPVIGFTMGWLIMTITKRLL
                     KNASPKADTALKVGQLPTAIALALSHGTNDAQKTMGIITMGLVVLGFQKEFVVPTWVI
                     LCSACAIGLGTALGGWRIIQTLGGKFYKIRPIHSFTSQFASALVIITASFLGGPVSTN
                     QVVSMSIIGAGASDKVSKVRWFVLKEIFTTWILTIPITATLSAIIYFIIDLIFK"
     gene            complement(4095..>5079)
                     /gene="galE"
                     /locus_tag="PLF48_08855"
     CDS             complement(4095..>5079)
                     /gene="galE"
                     /locus_tag="PLF48_08855"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNF49475.1"
                     /translation="GSHTIVDLLENGYEVICVDNLSRSSDYSLAALEKITGKNIRFYK
                     LELCDKEATAKFFEEHADAKGVIHFAALKSVPESVEKPLLYYENNLNSLLHVLEGIAR
                     HRIPSFVFSSSCSVYGNADELPVSESTPIKEAESPYAHTKQIGEEIIRHWNKSTRCNS
                     ILLRYFNPIGAHPSALIGEMPIDKPNNLVPYITQSVIGKLGALTVYGGDYPTRDGSCI
                     RDYIHVCDIAHAHTLALQYALQLHQSACEVFNLGTGKGVTVFEVINAFERATGKKLNY
                     MVGDRRAGDVVAVYADNTKAQTKLNWHCAYSLEDAMLHAWKWEQTMQSFKQ"
BASE COUNT         1595 a         1005 c          937 g         1542 t
ORIGIN      
        1 aaattataca gatgggatta aaaccggcaa atggaattat tggaataaat caggccaact
       61 aacaactaag gaagaatctg atgtgctatt agaagaatct gatttttata atatgcgtaa
      121 tatagatgtt gattttacag attacttttc agcacacaca catatagata tactgagctt
      181 tccggatgat attcggttgt atactgttcg agtttcgccg gagaggtatc cattaagttc
      241 atttttgccc gatacgattc atataaaagc tatcacagca tatgaccatc aatatatgct
      301 tgaccaagcc acaaactatc atcctgctga aaacgaatgt atgctactct atgaattgat
      361 taaacctccg gagttaaata tcaaacctat actgactttt ttattaggaa gttctgttaa
      421 aaacaaactg aaatctatta gcgaaagttc aattgatgat attataaaca tatatgaata
      481 ctcaataata aacgacgact atttaggttt ttcagctatg aaacaggcac tatataatgc
      541 aaaatagtta caactaaatc tagtagttac tacacaattc ttaataattc tttcttttaa
      601 gctcaagttt tctttatttc tttgttgtat gaaagcattt ctaatcaaaa tattagaaag
      661 ccactggttg cacaatttat tggatttgtc tgtaatcggg ctgattgtct atttctttta
      721 ccacagttat acatcagcag acgaaattcg gaagctacaa attctgtatc tgctggctat
      781 actgctggct gttcatgtac tggttcttct tgctcgtgta gctttccgta taaaaagaaa
      841 acgcattgta agcggatttt ccaattcggt atatttgctt atacaaatcg gctttttatt
      901 tgccgtattg tttaatattc atctgcaaag agggtagtta tggaaaaaca agttcttcat
      961 actgatgttg ccattattgg tgcaggatat gcgggtattg ctgccgcaaa actactgcaa
     1021 gcaaaccaaa ctgactttat cgtactggaa gcccgtgaca gaattggagg tcgtacgcac
     1081 tctgctacgt tggcatccgg agctcaggtt gaccttggtg cccaatggat cggccccact
     1141 caacaactta tttggcaatg ggtaaaagag accaataccg aaacgtatga aacttacgat
     1201 attgggaaaa acatcttgtc ttataaaaat aagacctcca cgtatacagg cacgattccc
     1261 aagattgatc ccatttcatt acttgatctg ggtttggcaa ttgaaaagat aaacaagctt
     1321 tgcaaacaga ttgatctcga aaaaccctgg caacacccga gagccttgga atacgactcg
     1381 atgactttcc acacctggat tgaaaaaaac atgcatacgg ccaaagccaa acatttgttt
     1441 catattggtg ttgaaacggt atttgctgaa gaagcttcct ctctttcttt tttacatgca
     1501 ttattttatt gccgtagtgg agaaaacatg gaatccctga tttctataaa aaacggggcc
     1561 cagcaaacac tactcaaggg cggcactcaa gctttattac aaaaaatagc tcagcccttt
     1621 tccgggaaaa taaaattaaa tcaagttgtc agccatattt accaatctga ttccggagtt
     1681 cagatagaga catcagattt tatcgtgcat gcaaagaaaa gcatccttac attaccccct
     1741 gctttattga atacaataaa attttcgcct cttctgccac aaaggaaagc acaactcata
     1801 cagcgtgtac ccatgggtgc tgcaatgaaa tgttttgtga tttataaaag tccgttttgg
     1861 agaaagatgg gctattccgg acaaattgtg agtgatatac acccggtgag ggtaactttt
     1921 gattgcactc acaaggatac tgacttgggt gttttgcttg tttttgtaga aggcaaaaat
     1981 gcccgttttt ttctcgattt aactcaagaa gaaagaaaaa aacaagtcgt ttcaatgctt
     2041 gtcaaatttt tcggggagga tgcagcatct tatgtagaat atactgataa atgttggaca
     2101 gaggaagaat acagccgggg ctgctatgca ggaaacatgc cacccggcgt gctaacacaa
     2161 tttggtgacg aattgagaaa acctttcatg catttgcatt ttgcaggtac agaaacggca
     2221 tataagtgga acggctatat ggatggagcg attgagagtg gtaatcgtgc aacggaagaa
     2281 gtactgaagc tgatataaaa aatgctcaat cataaagatt gagcataata tttaaagatt
     2341 ggttgttgta ttagtctaag tcagtttcat aatcacaata ccaaaaatat ttgctgccgt
     2401 atctgcacgg tctgccatgt tagataagtg acgatatact tcgcgtttgt aaaaaaactg
     2461 aggcaggttt tccggtgtaa ccggatggat aaataaatcg cgcaaggctt tgcgatattg
     2521 tttctccaca tctttttcca gcttcttcgt tttcaaggca tgctcatgag ctacgcgcgg
     2581 atttttgctg aggcgctcca tggccatcaa caactcttct gtctgtctac gaaccaaaac
     2641 aaccatttta cgtatatatt catccgccct tacattcaaa atgcgcatct catctgtagt
     2701 agtttgtgca taatccacca tatcaaacaa tgcatttgat atattataaa tatcctcacg
     2761 gtctatcggc gtgataaatg tattcaacaa atcatcaatc atcaccctgc gaatttcttc
     2821 aatttgtttg gtgatggtct ttaaatgttc cagcctggaa gcaatgtctt cttcatctaa
     2881 tgaatgttct aaaaaaacaa tagcttcgtt cgttttttgt gtttgttcga taagttgcat
     2941 gataaactta tctgtttttt cttctttaga gccgaaaatt cccataacta tttaaatatt
     3001 aaatctatta taaaatatat aattgccgat aaggtagccg tgattgggat ggttaaaatc
     3061 catgttgtaa aaatttcttt caaaacaaac catcttactt tcgacacttt atcactggca
     3121 ccagcaccaa taatggacat actcacaact tggttggtac ttacaggccc tcctaaaaat
     3181 gatgccgtaa taatgaccag tgcagaagca aattgtgaag tgaagctgtg aataggtctg
     3241 attttgtaaa atttaccgcc taaggtctga ataattctcc aaccgcccaa tgcagtaccc
     3301 aaaccgattg cacacgcact gcataagatc acccaggtgg gaacaacaaa ttctttctga
     3361 aaacctaata ccaccaagcc cattgtgata atccccattg ttttttgtgc atcattcgta
     3421 ccatgactta gggctaaggc tatggctgta ggtaactgtc ctaccttaag agcagtatct
     3481 gcttttggag aggcattttt aagcagtctt ttagtgatag tcatgatgag ccaacccata
     3541 gtgaagccga ttacaggcga agtaaataag gcaatcagaa taacataaat acctttaagt
     3601 tttatagcac caaatcccga atccataata acggcaccga taataccacc aattaacgca
     3661 tgagatgaac tggatggaat accaaaatac caagtggtta cattccacat gctggcactc
     3721 attagcgccg caataactac ataaatatta atggaactgg tgtctgccac attatgacct
     3781 atggttttag ctacggccac accaaaaata aacggaccga taaggttggc aactgcagcc
     3841 aacgtaaggg cagcccttgc agacatggct ctggatgaaa tgatagtggc cactacattg
     3901 gcactgtcat taaaaccatt taaaaaatca aaggtcaggg ataaaaccaa taatatgatt
     3961 agccaaattt caggtgtcat atgtgaatat ctgtgtgaaa cgaaagtgca aaaatacggt
     4021 ataagtcaaa taatttaatg acgagtatca ccaaaaaata caatttttat cattgatttt
     4081 tttctatatg aaatctactg cttaaaactc tgcatagttt gttcccattt ccatgcatgt
     4141 agcatggcgt cttctaagct ataagcacaa tgccagttta attttgtttg ggcttttgta
     4201 ttatccgcat ataccgccac tacatctcca gcccgacgat cgccgaccat ataatttaat
     4261 tttttaccgg tggctctttc aaaagcatta attacctcaa acacagtaac gcctttaccc
     4321 gtacccaaat taaatacctc acatgctgat tgatgtagtt gtaatgcata ttgcaatgcc
     4381 agcgtatgcg catgggcaat gtcgcatacg tgaatataat cgcggatgca actaccgtca
     4441 cgggtaggat aatcacctcc ataaaccgtt agtgcgccta gtttaccaat gacactttgg
     4501 gtgatatacg gtaccagatt attcggttta tcaatgggca tttcaccgat taatgcactt
     4561 ggatgtgcac ctattgggtt aaaataacgc aacagtatgc tattacatct ggtcgatttg
     4621 ttccaatggc gtattatttc ttcccctatt tgtttggtat gtgcataagg gctttctgct
     4681 tcctttatgg gtgttgattc tgaaaccggc aattcatctg cattgccata aacggaacaa
     4741 gaagatgaaa acacaaaact tggaatccgg tgtcgggcta taccttctaa tacatgcagt
     4801 aacgaattca gattgttttc ataatacaac aatggttttt ctacagattc aggaactgat
     4861 tttaaagcag caaaatggat aactcctttt gcatctgcat gttcctcaaa aaatttagcg
     4921 gtagcttctt tatcacacaa ctccaattta taaaagcgga tatttttgcc cgttattttt
     4981 tctaaggctg caagactata atcactggag cgcgacaaat tatctacgca aataacttcg
     5041 tagccatttt ccagtaaatc aactatggta tgcgaaccg
//
LOCUS       DAOQII010000187         5055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9721_length_5055_cov_4.933200, whole genome shotgun sequence.
ACCESSION   DAOQII010000187 DAOQII010000000
VERSION     DAOQII010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9721_length_5055_cov_4.933200"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PLF48_08860"
     CDS             complement(<1..1488)
                     /locus_tag="PLF48_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNF49476.1"
                     /translation="MTRLFDYLIENKNQLPEQEFLSYKSAKTGNWESYTFLETYKTVL
                     KTVQLLLDLGVKPKDNIALIATNCPEWNFVDMAVQMIDAVCVPMYPTISSSDYAYIFN
                     DAEIKIAFVGNESIYKRVEAVSGQLPLLRKIISFDKIKATDQYFSLLIGIKPDEKRIL
                     KIADAVSENDLATIIYTSGTTGNPKGVMLTHCNIMSNLEAVQQDLPLVEGKRSLSFLP
                     LCHSFERTVFYAYLANKIHIHYAESLEKIADNLKEVKPYCFTTVPRLLEKVYDKIMDK
                     AKDLDTVKRVLFDWSLKLGNEYEYGRDKNELVYGLQIILARAFVFSKWREALGGELEF
                     IVTGAAPIQPRLIKMFTAAGINVLEGYGLTETSPVLAFNRIEINDRKIGTVGIPVRGV
                     TIQLADDGEILAKGPNIMKGYYKRPDLTAEVIDKEGWFHTGDIGEWVENKFLKITDRK
                     KELFKTSGGKYIAPQVIENKMKESKYIEQIMVTGEYQKFISALIVP"
     gene            complement(1644..2186)
                     /locus_tag="PLF48_08865"
     CDS             complement(1644..2186)
                     /locus_tag="PLF48_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNF49477.1"
                     /translation="MASGKLKKEDCLLLVIDIQERLMPVIFHQKQVIRNTNVLLKGMQ
                     ILGVPVVVTEQYPKGLGNSCKEIELPEGQEIYEKITFSCVATEAVIEKLKAKKQIILL
                     GVEAHICVLKTALDLIEQGHEVHVVADAVSSRTTENKLYALQRLRQSGVYIETTEMVL
                     FQLLDQAGTEEFKAISKLIK"
     gene            complement(2370..4100)
                     /locus_tag="PLF48_08870"
     CDS             complement(2370..4100)
                     /locus_tag="PLF48_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNF49478.1"
                     /translation="MNGGEIIAHLLKKYDVEYMFTLCGGHISPIFVGAKDIGINVIDT
                     RHEANAVFAADAYARLTGKMGVAAVTAGPGVTNTITAIKNAQMAQSPVLLLGGAAATV
                     LKGRGSLQDIEQLAVMQSITKYCTSVRKVKDFVPVINTAIQIAMEGVPGPVFVECPID
                     LLYDEELVKSWYLASGNKKVSLTEQAVQWYLKRHVDSIFDGTKNIRIKEEKILPAKDN
                     HTDAHIRVVKSAISKSRKPLLLVGSGSLHDASHANDLANAVNKLGIPTYLSGMARGLL
                     GKDNKLQQRHNRRNALKQADLIILAGVPCDFRLDYGRHLSSKAKTVFINASKTDLFKN
                     KSPDIAVNSNPALFLSDLAKKFQPKNDWSEWFAILEKSDADKEVSIADHAKEKGEYIN
                     PVELFKTLDTLLPDNTTLVADGGDFVATASYILKPRHTLSWLDPGVFGTLGVGGGFAL
                     GAKIVNKEDEVWVIYGDGSSAFSIAEIDTMVRHNLPAIILIGNDASWAQIARDQVDIL
                     KSDVATVLSYSSYEKIGEAFGASGKTVRNLEEFTAAVKEAQACVKNGKPYVINALLNK
                     SDFRKGSISM"
     gene            4307..4816
                     /locus_tag="PLF48_08875"
     CDS             4307..4816
                     /locus_tag="PLF48_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49479.1"
                     /translation="MFDWVYIEQIPERDIHTLNLNLNEDLQGYRFFSYLFMGGALLFL
                     LIALLKVRILFPYIAISVLFFIGFFMYKKATNTYHYRISCFLNGDAITAIVLKQTRTF
                     NPFSSKRKYAIVLQYQNQLITVEHNSEKLWTEFPVKKEVIGLSYNNHFLFGPELGVDF
                     KFFLNKNNK"
     gene            4865..>5055
                     /locus_tag="PLF48_08880"
     CDS             4865..>5055
                     /locus_tag="PLF48_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49480.1"
                     /translation="MEEPAYLKKIFYACMMLVMRFIRYRENLNSQKILSVGETPTDID
                     SSLQDILLEVNYFAVISIV"
BASE COUNT         1493 a          995 c          834 g         1733 t
ORIGIN      
        1 agggacaatc aacgctgaaa taaatttttg atattcgccg gttaccataa tctgttcgat
       61 atatttcgat tctttcatct tgttttctat cacttgcggc gctatatatt taccacccga
      121 agttttaaac aattcttttt tgcggtcggt aattttcagg aatttatttt ccacccactc
      181 gccaatatct ccagtatgaa accaaccttc tttatctatt acttccgcag tcaaatcagg
      241 gcgtttgtaa tagcctttca tgatatttgg tcctttagcc agaatttcgc cgtcgtctgc
      301 cagctgtatt gtaacaccac gaacaggtat acctacggtt ccaatttttc tatcgtttat
      361 ttcgatacga ttaaatgcca gcacgggcga cgtctcagtc aaaccataac cttccaaaac
      421 attgatgccg gctgcagtaa acatcttgat tagccttggt tgaatcggtg ccgcccctgt
      481 aacgataaac tccaactctc ctcctaatgc ctcgcgccat tttgagaata caaaggcacg
      541 tgccaaaata atttgcaatc catagactaa ttcattttta tctcgtccgt attcgtactc
      601 atttcccagt ttcaatgacc aatcaaacaa cacgcgtttc accgtatcca gatctttggc
      661 tttatccatg attttgtcat acactttttc caacaagcgc ggtactgtcg tgaaacaata
      721 gggttttact tcttttaaat tgtctgcaat tttctctaaa ctttcagcat agtgaatatg
      781 tattttatta gccaaatatg cataaaaaac agtacgctca aagctgtggc acaacggcaa
      841 aaaactcagc gaacgctttc cttccacaag aggcaagtct tgttgtaccg cttcaagatt
      901 gctcataata ttgcaatgag tcagcattac ccctttagga tttcctgtag taccggatgt
      961 gtaaattatt gtagccaaat cattttcaga gacagcatct gcaatcttta gaattctctt
     1021 ttcatcaggc ttaatcccta taagcaaaga aaaatactga tctgttgctt ttattttgtc
     1081 gaatgaaatg attttgcgca gcaaaggcaa ttgaccactt accgcttcta cacgtttata
     1141 aatagattcg ttgccgacaa aggcaatttt aatttcagca tcattaaaaa tataggcata
     1201 gtcggatgaa ctaatggtag gatacatggg cacacaaaca gcatctatca tttgtactgc
     1261 catatccaca aaattccatt ccgggcagtt ggtggctatc agggcaatat tatctttggg
     1321 ttttactccc aaatctaata atagctgtac cgtctttaat actgtcttgt atgtttccaa
     1381 aaatgtatag ctctcccagt tcccagtttt agctgattta taactcagga attcctgttc
     1441 tggcagttgg tttttatttt ctatgaggta gtcaaataat cgagtcatgg aataatgttg
     1501 aatgatgaat tatgaatgtt gaaagataaa ttttaaatat agaattatga attacaatag
     1561 ctaaatatta gtatatatgt aataaattat attttattgc ttactttcaa atttactcat
     1621 ttacacattt gcacattgcc taattatttt atcaatttac tgatcgcttt aaattcttct
     1681 gttccggctt ggtctaacaa ttggaataag accatttctg ttgtttctat ataaacgccg
     1741 gattgcctca aacgctgcaa agcatacaat ttattttcag ttgttctcga tgaaacggca
     1801 tccgccacta catgtacttc atgtccttgt tcaattaagt ccaaagctgt tttcaaaaca
     1861 caaatatgtg cttctacacc aagcaatata atttgttttt tagctttaag tttttctata
     1921 acagcttctg ttgcaacaca actaaacgtg attttttcat atatttcttg accttcaggc
     1981 agttcaattt ccttacagga attgcccaat cccttgggat attgttctgt aactacaact
     2041 ggaacaccca gtatttgcat acctttcaat aaaacattcg tgttgcgtat cacttgtttc
     2101 tggtgaaaaa ttacaggcat caatctttcc tgaatgtcga taaccaagag taaacaatct
     2161 tcttttttta attttccgga agccatggga tgaatttgat aatgtgataa tgtgaaaatt
     2221 tgatgattaa gtatgaaaga taaacaatga atgatgaatg atgaatgatg aatgatgaaa
     2281 ttattagtga ttggtcatta gtcattggta actatggtct tgattcttgg ctcttggttc
     2341 ttgattcttg tttttctact tatgacctac tacatactta tcgaaccttt tctgaaatct
     2401 gatttattca gcaaggcatt aatcacatac ggctttccat ttttcacaca agcctgcgct
     2461 tctttcacgg cagctgtaaa ctcttccaag tttcgaaccg tttttccact cgcaccaaat
     2521 gcttctccaa ttttctcata agaagaatag gacagaaccg ttgccacgtc gctttttaaa
     2581 atatccacct gatctcgggc aatctgcgcc catgatgcat catttccgat aagaataatt
     2641 gccggtaaat tgtgtcttac catcgtatct atttctgcaa tggaaaatgc actcgaacca
     2701 tcgccataaa tcacccaaac ttcatcctct ttgtttacaa ttttcgcacc taaagcaaaa
     2761 cctccaccca cacccaaggt accaaaaacg ccggggtcta accaagataa agtatgtctg
     2821 ggtttaagaa tataagatgc cgtggccaca aaatcgccac catctgccac taaagtcgta
     2881 ttatcaggta acagcgtatc taatgtttta aacaactcga cagggttgat atattcgcct
     2941 ttttctttag catgatcggc tatgcttact tctttgtcgg cgtcactttt ttctaatatg
     3001 gcaaaccact cactccaatc atttttgggt tgaaattttt tagctaaatc acttaagaat
     3061 aaagcaggat ttgaatttac tgcaatatca ggcgacttat ttttaaataa gtctgttttt
     3121 gatgcattga taaaaactgt tttggcttta gaagaaagat gccgtccata atctaatctg
     3181 aaatcacatg gaactcccgc caaaattatc aaatcagctt gcttaagcgc atttctccgg
     3241 ttatgccgct gttgtagttt gttgtctttg cccaataaac ctcgagccat tcctgacaaa
     3301 tacgttggaa tacccaattt attcacggca tttgccaaat cattcgcgtg cgaagcatca
     3361 tgcaagctac cacttccgac cagcaacaat ggttttcgcg atttcgatat ggcagatttt
     3421 accactcgaa tatgtgcatc cgtgtggttg tcttttgctg gcaatatttt ttcttctttg
     3481 atgcggatat tttttgtgcc gtcaaaaatg gaatctacat gtctttttag ataccattga
     3541 acagcctgtt cggtaagtga gactttttta tttccagagg cgaggtacca gcttttcacc
     3601 aactcttcat cgtatagtaa atcaattgga cactcaacga acacggggcc cggcacacct
     3661 tccattgcaa tttgtattgc agtattgatt accggaacaa aatcttttac ttttcgcact
     3721 gaagtgcagt attttgttat tgattgcatc acggcaagct gttctatatc ttgcaaagat
     3781 cctctgcctt tgagtacggt agcggctgca ccacccagca acaaaacggg cgattgtgcc
     3841 atttgcgcat ttttgatagc tgtgatggta ttggtaacac cgggacctgc tgtaacggca
     3901 gccactccca tttttccggt aagccgagca taggcatctg cggcaaaaac ggcattggct
     3961 tcatgtctag tatctattac atttattccg atatcttttg cccctacgaa aataggcgat
     4021 atatgcccgc cacagagtgt gaacatatat tccacatcat attttttgag taggtgcgca
     4081 ataatttcac cgccattcat attttataat tttcttgtaa gttaagaaaa agaaagcaca
     4141 tattttatga tgttagtcat gctatttaat tgcaataaat agtatatagt attattttat
     4201 cagtttttta ttaaattatt atacgaacca cgaaagtatt ttgctaagca taaaaaaata
     4261 ttatcttagg tgtagtttaa tttctttatt taaaattgaa aaaatcatgt ttgactgggt
     4321 atatatagaa cagattccag agagagatat tcacactctt aatttgaatt taaatgaaga
     4381 cttacaaggc tatagatttt ttagttattt atttatgggt ggggcattac ttttcttatt
     4441 gattgcatta ctaaaagtac gtatcttatt tccgtacatt gcaatttccg ttttgttttt
     4501 tatagggttt tttatgtata aaaaagcaac taacacttat cattacagaa taagctgttt
     4561 tttaaatggc gatgctatta cagcaattgt attaaaacaa acaaggacat ttaacccatt
     4621 ttcgagtaaa cgaaagtatg ctatcgttct acaatatcaa aatcaactca ttacggttga
     4681 acataattct gaaaaattgt ggacagaatt tcccgtcaag aaggaagtta tcggattgag
     4741 ttacaataat cattttctct ttggacctga gttgggtgta gattttaaat tctttctgaa
     4801 taaaaacaat aagtaagatt ttgttttgta gaattgaaat gtatataaaa tctatttact
     4861 gaaaatggaa gaaccagctt atcttaaaaa gattttttat gcatgtatga tgttggtcat
     4921 gcgatttatt cgatatcgtg aaaatctgaa ttcacaaaaa atactttcag tcggtgagac
     4981 accgaccgat attgatagtt cattacaaga tattttgctt gaagtcaatt attttgcagt
     5041 aatctcaatc gtaat
//
LOCUS       DAOQII010000188         5009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9851_length_5009_cov_3.487687, whole genome shotgun sequence.
ACCESSION   DAOQII010000188 DAOQII010000000
VERSION     DAOQII010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9851_length_5009_cov_3.487687"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLF48_08885"
     CDS             <1..834
                     /locus_tag="PLF48_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49481.1"
                     /translation="LGWGLNSGADEMSFIPQKSKDDSVLISGYLVSNRKGGKGLDDIY
                     YVEKRMTPKEKLPPPVYVLRGTVQENSYEIENNPNSKVLGVKPIENAIVKLPENIKTN
                     TEGKFEYWMKQKQNFTYEVGKEGYLSRDVQVNMDNIQANDGDTIVINQTILLSKIYKN
                     VEIVLNNIYYDYDKWNIRKDAAQSLDTLVEILQKNPTIKIELASHTDCRGSDTYNQTL
                     SQKRAESVVAYLIEKEIDKNRLTARGYGESVPVEKCECTKCTEEQHQRNRRTTFKILS
                     E"
     gene            839..1288
                     /locus_tag="PLF48_08890"
     CDS             839..1288
                     /locus_tag="PLF48_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNF49482.1"
                     /translation="MSKSLFNPADQKEIFARLNQLNANSTRNWGKMTVSQMLKHMEIA
                     FSVPINKIQLPKDKLYYLAANPFARFFMIKMSSKWPKNMATVEDFKVKTDPDFVQAKT
                     AFLKIYQEFLKATDFSGCHPAFGVMDKDMWGIAMYTHLNHHLEQFGA"
     gene            1288..2463
                     /locus_tag="PLF48_08895"
     CDS             1288..2463
                     /locus_tag="PLF48_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNF49483.1"
                     /translation="MENQKRYDLRGVSATKDDVHAAIKNADKGLFPKAFCKIFPDFLG
                     KDEAFCNIIHADGAGTKSVLAYLYWKETGDLSVWKGIAQDAIVMNTDDLICVGATSDF
                     LLSSIINRNKHLIPGEVIKAIIDGTNEIVELFHQNGIEIHAMGGETADLGDSVKTIVV
                     DAVMTSRMKKSEVISNEKIKAGNVIVGLCSYGQATYESAYNGGMGSNGLTSARHDVFS
                     KIYAEKYPEAFDSEMPYDLVYSGSKLLTDEIEDSPLDAGKLVLSPTRTYAPVIKQIIK
                     EIGAEQINGMIHCSGGAQTKILHYIDHLHIIKDNLFEIPPLFKMIQEESNTDWKEMYQ
                     VFNCGHRMELYVDEKFADTIIRISNSFNIPAQIVGRVENAAAKQVSIKSKFGEFVYI"
     gene            2485..3198
                     /locus_tag="PLF48_08900"
     CDS             2485..3198
                     /locus_tag="PLF48_08900"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNF49484.1"
                     /translation="MPNAYFNFKQFTVYHDKCALKVCTDSCLFGAWAVAFTKEAYTIL
                     DIGSGSGLLSLILAQKTNAEITGVELDESSFLQAQVNVKQSPWPDKIKLVHGDCKNFF
                     PTEKFDFIICNPPFYEQMLSSEKNNEKQAKHDATLLLSDLISIFNRLLSPKGKLAILF
                     PYYRKVELLKAMSMNGYYLHAGVDIRPAPQKDNFRFAGIFGHAESVMPSEIAISIKDE
                     LQHYTPEFKALLKDYYLYL"
     gene            3240..3695
                     /gene="purE"
                     /locus_tag="PLF48_08905"
     CDS             3240..3695
                     /gene="purE"
                     /locus_tag="PLF48_08905"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNF49485.1"
                     /translation="MKVLILMGSSSDETVMQESVNYLNWFGIVNEMHVASAHRDPHKV
                     FELTSTAQENGFACIIAAAGMAAALPGVVAAHTSLPVIGVPLEGGLPGGIDALYSIVQ
                     MPAGLPVATVAVGKTGARNAAVLAARIIALQDKNTASKLEEFKAKGYKL"
     gene            complement(3887..4720)
                     /locus_tag="PLF48_08910"
     CDS             complement(3887..4720)
                     /locus_tag="PLF48_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF49486.1"
                     /translation="MAVRKFNYQFDEVKYYRCSVYRLGSNVQTVYVYVIDDILIDTAQ
                     RHNRENIDKILTENKINKILLTHFHEDHSGNVNYIVNMLNIDAYAHPKACEILRKGYK
                     MSPLGSLLSGHVENAHLKPLTENETVMTAHFKLQPVYTPGHCDDHYCYFEKDKGYLFS
                     GDIYVADKIKYFAPYENIFEQIRSLEKLLGLDFDVLFCSHNPKTQNGKQHIANKLQFF
                     QDFTGTVSNYFRQGHNTSKSILELMQLKENHFYKVITLGNFTTENMIKSVLKGLKQPV
                     K"
     gene            4814..>5009
                     /locus_tag="PLF48_08915"
     CDS             4814..>5009
                     /locus_tag="PLF48_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49487.1"
                     /translation="MKKYITAGLLLAALIAGITFFITKTMMQMEIDKAVQIHINDKVP
                     LSAQIDTSILISLMNDLQTKI"
BASE COUNT         1681 a          849 c          998 g         1481 t
ORIGIN      
        1 ttgggatggg gattaaacag cggagcggat gaaatgagtt ttattccaca aaaatctaaa
       61 gatgattcgg tactgatttc aggttatttg gtcagcaacc gcaaaggcgg aaaaggcttg
      121 gatgatattt attatgtaga aaaacgcatg acaccaaaag aaaaactgcc gcctccggta
      181 tatgtattgc gtggtacggt gcaagaaaac agttatgaaa ttgaaaataa tcccaattca
      241 aaagtgttag gggtaaagcc cattgaaaat gcaattgtaa aattaccgga aaatataaaa
      301 acgaacacag aaggaaagtt tgaatactgg atgaaacaaa agcaaaactt cacctacgag
      361 gtgggcaaag aaggatatct gagccgtgat gtgcaggtaa atatggacaa tattcaagct
      421 aatgatggtg ataccattgt catcaatcag acgatattgc tttctaaaat ttataaaaat
      481 gtcgagattg tgttgaataa tatttattat gattacgaca aatggaatat ccgcaaagat
      541 gctgcgcaaa gcctggatac actcgtggaa atattacaaa aaaatccgac tataaaaatt
      601 gaactggcat ctcatacgga ttgccgcggt tcggatacgt ataatcaaac cttatctcaa
      661 aaacgtgcag aaagtgtggt ggcgtatttg attgagaaag agatagataa aaaccgactg
      721 actgcaagag gctatggcga atctgtacct gtagaaaaat gtgagtgtac aaaatgtacg
      781 gaagaacagc accagagaaa cagaagaaca acttttaaaa ttctaagcga ataaatttat
      841 gtcaaaatcc ctatttaatc ctgctgatca gaaagaaatt tttgcacggc taaaccagct
      901 taatgccaat tcaacccgaa attggggtaa aatgactgtt tcgcaaatgc tgaaacacat
      961 ggaaattgca ttttctgtcc caattaataa aatacaattg cctaaagaca aattgtatta
     1021 tctggctgcc aatccttttg cccgtttttt tatgataaaa atgtcgagca aatggcctaa
     1081 aaatatggct acggtcgaag attttaaagt gaagaccgat cctgattttg tacaagccaa
     1141 aaccgctttt ttgaaaatct atcaggaatt tttaaaggca acagattttt cgggttgtca
     1201 tcctgccttt ggcgttatgg ataaagacat gtggggcatt gccatgtaca cgcacctaaa
     1261 ccatcattta gaacaatttg gcgcataatg gaaaatcaaa aaagatatga cctgcgaggt
     1321 gtttctgcta caaaagatga tgtacatgct gccataaaga atgctgacaa agggcttttc
     1381 ccgaaagcat tttgcaaaat atttcccgat tttttaggta aagatgaggc cttctgtaat
     1441 attattcatg cagatggagc cggtacaaaa tctgtgttag cttatctata ttggaaagaa
     1501 accggcgatt tgtcggtttg gaaaggtata gcacaagatg ccatcgtaat gaatacggac
     1561 gatttaattt gtgtgggagc aacatccgat ttccttttat cttctattat caacagaaat
     1621 aagcatttaa ttccgggtga agtcataaaa gctattattg acggaacgaa tgaaattgtc
     1681 gaactatttc atcaaaatgg gattgaaatt catgccatgg gaggtgaaac tgctgattta
     1741 ggcgatagtg taaaaaccat tgtggttgat gcagttatga caagcagaat gaaaaaaagc
     1801 gaggtgattt ccaatgaaaa aataaaagcc ggaaatgtca tcgtgggatt gtgttcttat
     1861 gggcaagcaa cctacgaatc agcatacaac ggaggcatgg gcagcaatgg actcacttca
     1921 gcccgacatg atgttttttc aaaaatttat gccgaaaaat atccggaagc cttcgacagt
     1981 gaaatgccgt atgatttggt ttacagtggc tcaaaattgc tgaccgacga aattgaagat
     2041 agtccactgg atgcaggaaa attagtgctt tcaccaacaa ggacttatgc ccctgtaatt
     2101 aaacaaataa ttaaagaaat aggagcggag caaatcaacg gaatgataca ttgcagcgga
     2161 ggcgcacaaa ctaaaatttt gcattatatc gaccatttgc acattataaa agacaattta
     2221 tttgaaattc cgccactttt caaaatgata caggaagaaa gcaatacaga ctggaaggaa
     2281 atgtatcagg tgtttaattg cgggcaccgc atggagcttt atgtagacga aaaatttgct
     2341 gacactatca tccgtatttc caattcattt aatattccgg cacaaatagt cggaagggta
     2401 gaaaatgctg ctgctaaaca agtaagcata aaaagcaaat ttggcgagtt tgtttacata
     2461 taattttgat aaacaattga ttatatgcca aatgcatact ttaactttaa acaatttacc
     2521 gtctatcatg ataaatgtgc actaaaagtt tgcaccgatt cttgtttgtt tggtgcgtgg
     2581 gcagttgcat ttaccaaaga ggcgtatacg attttagata ttggcagtgg cagcggatta
     2641 ttaagcctca tattagctca aaaaactaat gctgaaatca caggagttga gctggatgaa
     2701 agttcatttc tacaggcaca ggtaaacgtt aaacaatcac cttggccgga taaaattaag
     2761 ctggttcatg gggattgcaa aaactttttt ccgacagaaa aatttgattt tatcatttgc
     2821 aacccgcctt tttatgagca aatgttgtca tctgaaaaaa ataacgagaa gcaagcaaaa
     2881 catgatgcca ccttattgct aagtgattta atctcaatct tcaacagatt actttctcct
     2941 aaaggcaagc tggctatttt atttccatat tacagaaaag tagaattgct gaaagcaatg
     3001 agtatgaacg gctattattt acatgcaggt gtagatatcc gtcctgcgcc acagaaagac
     3061 aatttccggt ttgcaggtat ttttggtcat gctgaaagtg taatgccatc cgaaattgct
     3121 attagtataa aggacgaact gcagcattat acacctgaat tcaaagcctt gttgaaagat
     3181 tattatctct acctctgaaa aataaaaatc atagattatc ttcgccaaaa atcagaaaaa
     3241 tgaaagtatt gatattaatg ggctcatcaa gcgatgaaac agttatgcag gaaagtgtaa
     3301 actatttgaa ttggtttggg atagtaaatg aaatgcatgt tgcttccgcc caccgtgacc
     3361 cgcataaagt atttgaactg acgagtactg ctcaagaaaa cggatttgct tgcattattg
     3421 cagctgcagg catggcggct gctttgcctg gcgtggtggc agcacatacc tcacttcctg
     3481 ttatcggggt gccgttagaa ggtggtttgc ccggtggcat cgatgcactt tattccattg
     3541 tgcaaatgcc ggcaggacta ccggtagcta ctgtggctgt aggcaaaaca ggtgcaagaa
     3601 atgccgcagt gctggccgcc agaattattg cacttcaaga taaaaataca gctagtaaat
     3661 tagaagaatt taaagcaaaa ggatataagt tataaaggag cataatttat tattaacctg
     3721 agttcgggtt aagaaaaata agctgataaa atagttgatt tctcattttt tcctccactt
     3781 taagaggtgg aggaattaaa aggaggtgct ttctgataaa aaatcaatta attttaattg
     3841 aaactcttat actgaactca ggttattata tttctttaat ttatactcac ttgaccggct
     3901 gttttagtcc tttcagcaca gattttatca tgttttctgt ggtaaaattg ccgagtgtaa
     3961 tcactttata aaaatgattt tctttgagct gcatgagttc cagaatagat ttgctggtgt
     4021 tatgcccttg tcggaaatag ttgcttaccg ttccggtaaa atcttgaaaa aactgtaatt
     4081 tgtttgcaat atgttgtttg ccgttttgtg ttttgggatt atgcgaacag aacagcacat
     4141 caaaatctaa acctagcagt ttctccaatg aacgtatttg ttcaaatata ttttcatagg
     4201 gagcgaagta tttgatttta tcggcaacat aaatatcccc tgaaaacaaa tagcctttat
     4261 ctttctcaaa ataacagtaa tgatcatcac aatgacctgg tgtatatacg ggttggagct
     4321 tgaagtgtgc agtcataacc gtttcatttt cagttaaagg ttttaaatgg gcattttcta
     4381 catgtccgct caataaggag cccaacggcg acattttata accctttcta agaatttcac
     4441 aggctttggg atgagcgtaa gcatcaatat tcagcatgtt gacaatataa ttgacatttc
     4501 cgctatggtc ttcatgaaaa tgagtgagca gtatcttatt tatcttgttt tctgtcagta
     4561 ttttatctat gttttctcgg ttatgccgtt gagccgtatc aattaaaatg tcgtcaatca
     4621 catatacgta aacggtttgt acatttgaac ctaaccggta tacactgcat ctgtaatact
     4681 ttacttcatc aaactgataa ttgaattttc ttaccgccat gatgcaaaac taagtatcgc
     4741 aaagcagaat ttactaaaca caagttatca ttttacaaaa aagattttta acttgttaga
     4801 caattttatc actatgaaaa aatatattac agctggatta ttgttagctg ctttgattgc
     4861 agggattacg ttttttatta caaaaaccat gatgcaaatg gaaattgaca aagctgtgca
     4921 aatacatatc aacgacaaag tgcctttgag tgctcaaatt gatacctcca tcttaatttc
     4981 actgatgaat gatctgcaaa ctaaaattc
//
LOCUS       DAOQII010000189         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9893_length_4996_cov_5.370370, whole genome shotgun sequence.
ACCESSION   DAOQII010000189 DAOQII010000000
VERSION     DAOQII010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9893_length_4996_cov_5.370370"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLF48_08920"
     CDS             complement(<1..1018)
                     /locus_tag="PLF48_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49488.1"
                     /translation="MRILISLTIALLGFSNIMTGQTISLTQHGNNSFCYSGIDSFLVS
                     WSNIPAGSNIVFYQSTNPNFNPYAGQGDSIGFIHIDNSNTSNGQPITSTCPRIFGIFI
                     DACNDNGRSEPANEYILMTSGKGFKVSNLQIDIPNNTDINFGTNPCTFATPNAATMNL
                     FYQQSCNAANILPAGQADSIPPDALVIVFTGNGVDFAYNFNELCNTGQKVYILQSSCT
                     HSPTSGSFANNAPNQTPCVSSYRTTTIYNRNCTDKLAYDRCGLPVFDNANPNAGDGDY
                     VIHLDNTDTSSVANGGVLNNTTNKCNGVILDSVVGAKYIAYTVPNGQNGNPLNFCNTG
                     THYIK"
     gene            complement(1022..4297)
                     /locus_tag="PLF48_08925"
     CDS             complement(1022..4297)
                     /locus_tag="PLF48_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HNF49489.1"
                     /translation="MKNLFLASFLSFIAFFVSAQTQINLSPSTSVICSTGHDTLFYNV
                     SASGLPPNTNVVIYQSRDSTFNPYLQQGDSIGYIPGNALPVDTINFGSCIKTLGIFID
                     ACGATGQEPKNEYMVLTSGSGIRVTNLAINFDPANSNSGNNNDNDINLGTNPCGFKTP
                     DAALIANLQVGSCNAANIIPVSPTDSIPPNAIILCFTSDSVNTIYNVNGLCNLGYPIY
                     VVQSSCTRTIGAFTNAPSCSSTATTRYRKTIAIDTRQNCQDNFVYDRCGIFNLDGTYA
                     IRQQGTDTARVSNNGIRRNTVDSCGGIDYTKLIFNSDTLLKLPLSTNLCNAGKYFIKA
                     ITHPEGTQPISNTISYELKCVDVNTNTTRQEFCSGNTTNIQIDGTDPNATYSWTVSGG
                     AGISGATNGTGTSIQQTLTYTGTSKDSLTYTITASDGNCTKSTQVNVVVKNCDTCNLG
                     FQINGNTSICNGQSTILSVAVQADSLRWNTGASSNSITVSQAGTYSVTVYKNGCSKTE
                     AVTVNVNQLSVAISGNRTFCSGQSTTLVAEGAYDSLRWSNGISTTFNTISQPGTYTVV
                     AYQNGCLASTAVTVSVQQVDVNITGDTILCNGESTTISIAGQFDSVRWSTGETTNSII
                     ISSAGTYSVLGYLNGCSGVDIVTVESSNLSVNITGTDVICGGTTATLTAIGVYDSLRW
                     NTNETSASINVTESGRYSVTAFLGACSISASFYVARVSIDYFLPKKVDSICQGDTARF
                     ILFAGDLGAAPVDTFYFTQPGRYLVSYTTTICGVFYDSVFVYEKHIQKPNITGNLQIC
                     GQQTTTLDAGLGYETYQWLPNSETTQTITTANTGTYIVTGFKGRCFASDTVVVQSAPL
                     PPSFHLGNDTTYCNNFSRTLSTGDATTVWSTGATAATITVTGAGTYVATITNACGFTS
                     DTIIIKLANSPILNLGNDTTICNSNLILHAPMNMESYLWSTQETTSSITVNTDGTYWV
                     VVTDSNHCQAVDSISIGNNCMHDVWIPTAFSPNGDGINDVFMVRSSGNFITVKKMLIY
                     DRWGQLLFSAYDVLANDVSKGWDGTYKGKIAQLESYGFVIETEDTSVGMKKTFKGNVT
                     IIR"
     gene            4483..4971
                     /locus_tag="PLF48_08930"
     CDS             4483..4971
                     /locus_tag="PLF48_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49490.1"
                     /translation="MKKLFFLLLFCPVIALHAQNKSKTIAFPTFKLLAMDSTAFVENN
                     QLNDTLITLFINFSPECDHCERTIKSILDNISKFQQTQIVLTSFEPFDKIRKFYLDNS
                     LYAYKNLFVGQEIDYQLTKQIHYAGFPSVVMFDKNKQWIKSIHEETNAKTILKALKLK
                     YK"
BASE COUNT         1561 a          941 c          877 g         1617 t
ORIGIN      
        1 ctttgatata atgcgtacct gtattgcaaa aattcagcgg attaccgttt tggccgtttg
       61 gtacggtgta agctatatat tttgcaccaa caacagaatc taaaataaca ccattgcatt
      121 tatttgtggt attattcaaa actccgccat tagcaacact ggatgtatct gtattatcga
      181 gatgaatgac ataatcgcca tctccggcat ttggattagc attatcaaaa acaggcaatc
      241 cacatctgtc ataagccaat ttatctgtac agtttctgtt gtaaatggta gtggtacgat
      301 aactgctgac acaaggtgtt tgattgggag cattattagc aaaagacccc gaagtaggac
      361 tatgtgtaca agaactttgc agaatataaa ctttttgccc ggtattgcag agttcattaa
      421 agttgtaggc aaagtcaacg ccattaccgg taaatacaat tactaatgca tcaggtggaa
      481 tagaatccgc ttgtcccgct ggtaagatat tagcggcatt gcaggattgc tgataaaaca
      541 agttcatcgt agctgcattg ggtgttgcaa aagtacatgg attagtacca aagttgatat
      601 ctgtattgtt cggaatatct atttgtaaat tactgacttt aaaacctttc ccggaggtca
      661 ttaatatata ttcgttggca ggttcagacc gaccgttatc attgcatgca tcaataaaaa
      721 taccaaatat tcgagggcat gttgatgtaa tcggttgtcc attactggta ttactgttgt
      781 caatatgtat aaatcctata ctgtctccct gtcctgcata tggattaaaa tttgggttag
      841 tagactgata aaacacgata ttgctgcctg ccggaatatt cgaccaggaa accagaaatg
      901 agtcaattcc tgaatagcag aaactgttat tcccatgttg tgtaaggctg atagtttgtc
      961 ctgtcataat attgctaaat cccagcaatg caattgtcag gcttattaaa attctcatcg
     1021 tttatctgat tattgtaaca tttcctttaa acgttttttt cattcccaca ctggtatctt
     1081 ctgtttcgat tacaaaacca taagattcca gctgagctat tttaccttta tatgttccat
     1141 cccaaccttt acttacatca tttgccagaa catcataagc actgaaaaga agttgtcccc
     1201 agcgatcata aatcagcatt tttttaacgg ttataaaatt cccggaactt ctgaccataa
     1261 atacatcatt gatgccatca ccattgggcg aaaatgcagt tggtatccag acatcatgca
     1321 tacaattatt tccgattgaa attgaatcaa ccgcttgaca atgattggaa tctgtaacaa
     1381 ctacccaata cgtaccgtcg gtattaacag taatagaaga agttgtttcc tgcgtactcc
     1441 acaaatagga ttccatattc ataggtgcat gcaaaataag attgctgtta cagatagtcg
     1501 tatcatttcc taaattcagg attggactgt tagctagttt tatgataatt gtatcgctgg
     1561 taaaaccaca agcattagtg attgtagcaa catacgtacc tgcaccggtt acagtaattg
     1621 ttgctgctgt tgcaccagta ctccacacag tggttgcatc accggtagaa agtgtgcggg
     1681 aaaaattatt acagtatgtt gtatcatttc caagatgaaa tgaaggagga agtggagctg
     1741 attgaacaac gactgtatcg cttgcaaaac aacgcccttt aaatcctgta actatgtacg
     1801 tacctgtatt tgcggtagtt attgtttgag tagtttcact attcggcagc cactgataag
     1861 tttcatatcc tagaccggca tctaaggttg tagtctgttg accgcaaatt tgcaagttac
     1921 ccgtaatatt tggtttttga atatgctttt cataaacaaa aacactgtca taaaaaacac
     1981 cacaaattgt agtagtatag gaaactaaat atctaccggg ttgggtaaaa taaaaagtat
     2041 caaccggagc tgcacctaag tctccagcaa ataaaatgaa tctagctgta tcaccttggc
     2101 aaattgaatc aacttttttg ggtaaaaaat aatcaatgct tacacgggct acataaaaag
     2161 aagcactaat agaacaagcc cctaaaaatg ctgttacact atacctgcca ctttctgtaa
     2221 cattaataga agcactggtt tcatttgtat tccaacgtaa tgaatcgtaa acaccaattg
     2281 cagtgagcgt tgcagtagta ccaccacaaa tcacatctgt tccggtaata ttgacactta
     2341 aatttgaact ttcgacagta actatatcca cacccgaaca accattaagg tagcctaata
     2401 ccgaatatgt acctgcactt gatatgataa tagaattagt tgtctctcca gtactccagc
     2461 gaacagaatc aaattgtcca gcaatagaaa tggttgtact ttctccgtta caaagtatcg
     2521 tatctccagt aatattgaca tcaacttgtt gtacacttac cgttacggca gtacttgcca
     2581 aacatccatt ttgataggca actacagtat aagttcccgg ttgtgaaatg gtattaaatg
     2641 tcgttgatat accattgctc catctcagtg aatcataagc accttctgca actaatgtag
     2701 tgctttgacc actacaaaaa gtgcggtttc ctgatattgc aacactcaac tgatttacat
     2761 taacggttac cgcttcggtt tttgaacatc catttttata aaccgttacg gagtaagtac
     2821 ctgcttgaga aactgtgata gaattagatg aagctcctgt attccatcgc aaggaatctg
     2881 cctgcacggc aactgacagt atagtagatt gcccattgca aatactcgta tttccgttaa
     2941 tttgaaatcc cagattacaa gtatcacaat ttttcaccac cacatttact tgtgtagatt
     3001 tggtgcaatt gccatcagaa gctgttatgg tataagttaa actgtcttta cttgttccag
     3061 tataagtaag tgtttgttgt attgatgtac cagttccgtt tgttgctcct gaaatacctg
     3121 ctcctccact gaccgtccag ctataggttg cattcggatc tgtgccatca atttgaatat
     3181 tggttgtatt tccactgcaa aattcctgac gtgttgtatt tgtatttaca tctacacatt
     3241 tcaattcgta gctaatcgta tttgatatgg gttgagtgcc ttccggatgg gtaatggcct
     3301 taataaaata ttttccggca ttgcacagat tagtacttag cggtaatttt aatagtgtat
     3361 ctgagttaaa aatgagtttt gtataatcta taccgccaca gctgtcaacc gtatttcttc
     3421 tgataccatt attgctcact ctggcagtat ctgttccttg ctgacgtatg gcgtatgttc
     3481 cgtctaaatt aaaaatgcca catctgtcat acacaaaatt atcctgacaa ttttgtctgg
     3541 tgtcaatagc aatggttttt cggtaacgtg tagttgcagt agatgaacaa cttggggcat
     3601 ttgtaaatgc accgatcgtt ctggtacaac tgctttgcac tacatagatt ggataaccta
     3661 agttacacaa tccattcaca ttgtatattg tattgacaga atcactggta aagcacaaaa
     3721 taattgcatt gggcggaata gaatcagttg gtgaaaccgg aataatatta gcagcattgc
     3781 atgaacctac ttgcaaattt gcaattaatg cagcatccgg agttttaaat ccacatgggt
     3841 tagtacctaa attaatatca ttatcattat tgttaccgct attgctgtta gccgggtcaa
     3901 aatttatggc cagattagtc actctaattc cagaaccgga agtcagaacc atgtattcat
     3961 ttttaggctc ttgccctgta gcaccacaag catctataaa aatacccaat gtttttatac
     4021 aagagccaaa attaattgta tctaccggaa gtgcgttgcc cggaatatat cctatactat
     4081 caccttgttg taaataggga ttaaatgtac tgtccctgct ttgatagata actacattgg
     4141 tatttggtgg caatccactt gctgacacat tataaaataa ggtatcgtgg cctgtagagc
     4201 aaattacaga agtagatggt gataagttta tctgagtttg tgcagatacg aaaaatgcaa
     4261 taaaactcaa aaaacttgct aaaaacaaat ttttcataaa tgtaattcta taacccgtaa
     4321 agttacgaat tgctatccat ttatttgtaa tgacgcatat tgaaacgagt attttcggaa
     4381 ttaaataaca tctttatgcc agatatccac aatgtttcat ccaatctaat gccttatttg
     4441 atttatatct aatttttggt ttgatttttg tattttacag gtatgaaaaa attatttttt
     4501 ctcctcttat tctgtccggt aattgcactt catgctcaaa ataaaagtaa gacaattgcg
     4561 tttcctacgt tcaaactttt ggcaatggac agtactgctt ttgtagaaaa caatcagctc
     4621 aatgatacat tgattacttt gtttatcaat tttagccctg aatgcgacca ttgcgaacgc
     4681 acgattaaaa gcattttgga taatatatct aaatttcagc aaactcaaat cgtattaact
     4741 tcatttgagc cttttgataa aatcagaaaa ttttacttgg ataattcatt gtatgcatat
     4801 aagaatttat ttgtagggca agaaatagat tatcagttaa ctaaacaaat tcattatgcc
     4861 ggatttccat ctgttgttat gtttgacaaa aacaaacaat ggataaaaag tatacatgaa
     4921 gaaacgaatg ccaaaactat tttaaaggcg ttaaagctta agtacaagta atttcttttt
     4981 tctctaaatc tttatt
//
LOCUS       DAOQII010000190         4986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9922_length_4986_cov_4.849523, whole genome shotgun sequence.
ACCESSION   DAOQII010000190 DAOQII010000000
VERSION     DAOQII010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9922_length_4986_cov_4.849523"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..1080
                     /locus_tag="PLF48_08935"
     CDS             34..1080
                     /locus_tag="PLF48_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNF49491.1"
                     /translation="MQHLSQEEKDFYRRQLILPGFGESAQQKLKSATVLIVGAGGLGC
                     PVAQYLSAAGVGKIVIADGDTVAMHNLHRQVLYTIDDIGKNKALAAVEKLQKQYPHVQ
                     LIAFPYMLSNDNINSLINDATAVCDCSDNFSTRYLVNDQCVQYKKPLISAAVAEYSGQ
                     LSVFSHKTNINYRDVFAEEDATSQTCANAGIWGALCGIIGSMQANEVIKIITEIGDTL
                     EGKLLTYDALTHKMHIFKISHNKEQYIYASITDNATHIEKNTKILNSATFHQLKQQKQ
                     EIYLIDVREAWEYEESNFGGINIPLVELTSHMHEFEKEDVLVFYCHSGKRSQTAVRLF
                     LSKGYDQLYIFLPD"
     gene            1399..3822
                     /gene="lon"
                     /locus_tag="PLF48_08940"
     CDS             1399..3822
                     /gene="lon"
                     /locus_tag="PLF48_08940"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNF49492.1"
                     /translation="MLEFSNIIEQDVEMIPLLSIEEDDNIKSSKLPKILPILPLRNNV
                     LFPGVVIPISVGREKSIKAVKKAYKENKYIGVIAQRDPKVEEPEPGDLYSVGTTAKII
                     RILNMPDGGNTVILQGVHRFIAKTLISSDPIIQTEVEYLEDVPLSDKTQEKALIASMK
                     DMAGRIIELSNHIPNEAKFILNNINGLKFLANFITSNIPIELAQKQQILETNSVEERV
                     QQVLKYLNQELQLQEIKANIQEKTKTDIDKQQRDYFLNQQMRAIQEELGGDMYQEEIN
                     RLKEKAKEKKWSEAVAKQFDKEIGKLQRINPAAAEYSVILNYLDLMVELPWNEITKDN
                     FDLKRAGKILDEDHYALDKIKDRIIEYLAVLKLKGDMKSPILCFVGPPGVGKTSLGKS
                     IARSLDRKYVRVALGGLHDESEIRGHRKTYIGAMPGRIIQSLKKVQTANPVFVLDEID
                     KVGKDHRGDPSSALLEVLDPEQNTTFYDNYLEQEYDLSKILFIATANSLNEIPHALRD
                     RMEVIQLSGYSLEEKTEIAKRHLVAKQREAHGLKASQIKFNDKVIQFIIHHYTYESGV
                     RELDRMIASMMRNIAKCIATDESYNVQVTTEDVARILGKFRYNHDKYTQNNPCGVAIG
                     LAWTPHGGDILFFESTVSRGKGNFTLTGNLGNVMKESFTTAFTYLQSKADSLQIPVKA
                     FSDWNLHVHVPEGAIPKDGPSAGIAIYSALASLFTQRKLKPFTALSGEITLRGKVLPV
                     GGIKEKILAAKRAGIKEIILCKENQKDVDDVPQDFVKGLKFTYVEDMDEVIELALEKK
                     KIPDAIHFE"
     gene            4165..4515
                     /locus_tag="PLF48_08945"
     CDS             4165..4515
                     /locus_tag="PLF48_08945"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF49493.1"
                     /translation="MLYRIFTINKISIMLYHESDYYEEDIQLAEVCKAIAHPARIQIL
                     RIIVAKQNCTVSHLVKKMNLAQSTISQHLKALKDAGIIKTSNLGVSTVLSVKIKSLKN
                     AEKAMKFLTQALDK"
     gene            complement(4598..>4986)
                     /locus_tag="PLF48_08950"
     CDS             complement(4598..>4986)
                     /locus_tag="PLF48_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49494.1"
                     /translation="EMTVIFWIEKNWSRQRAVTIGVLMHVIAYLTAVVFHLDFFGAWV
                     MMSFITLAEMFSFSVLFNYWVSRTTDSTRGQYAGIWTMTWAFSSSLGPFGGSWIAEQY
                     GFTVLWSIVAGISLTAAFLYNRLITD"
BASE COUNT         1705 a          912 c          971 g         1398 t
ORIGIN      
        1 tatttctata cattcattgc ttacctttaa gccatgcaac atctgtcgca ggaagaaaaa
       61 gatttttacc gacgccagct tatcctcccg ggatttggcg agtctgcaca acagaagtta
      121 aaatctgcta ctgtattgat tgttggagca ggtggcttag gctgtccggt agcgcaatac
      181 ctaagtgctg caggagttgg taaaattgta atagcagacg gcgataccgt tgcgatgcat
      241 aatctgcacc gtcaggtctt atacactatt gatgatattg gcaaaaataa agcgctggca
      301 gctgttgaga agttacaaaa gcaataccct catgtgcagc ttattgcctt tccctatatg
      361 ctgagcaatg acaatataaa ctcactcatt aatgatgcta cagcagtctg cgattgcagc
      421 gataacttta gtactcgcta tctggtgaat gatcagtgtg tgcaatataa aaaaccactt
      481 atcagtgcgg cagtagccga atacagcgga caattatctg tttttagcca taaaacaaac
      541 atcaattacc gggatgtctt tgcagaagaa gatgccactt cacaaacttg tgcgaatgcc
      601 ggtatatggg gtgctttatg cggtatcata ggcagtatgc aagctaatga agtcattaaa
      661 attattactg agattggtga tacgctggaa ggtaaactgc tcacgtatga tgcacttacc
      721 cataaaatgc acattttcaa aatcagccat aataaggaac aatatatata tgcaagtatt
      781 accgacaatg ctacacatat cgaaaaaaac acaaagattt tgaattcagc cacttttcat
      841 caacttaaac aacaaaaaca agaaatatat ttgattgatg tgcgtgaagc ttgggaatac
      901 gaagaatcga attttggggg aatcaatata cctttagtgg aattgacaag ccatatgcat
      961 gaattcgaaa aagaagatgt actagtgttt tattgtcatt cgggaaaaag aagccaaaca
     1021 gcagtccgct tatttttaag caaaggctat gatcagctct atatttttct tcctgattga
     1081 ttgaactgtt cttttggctt tatttctgtc ggtgagaaca attaccaatc tgagttcggg
     1141 ttaagaaaaa atagggagaa aaaaatagtt gatttctcct tttttcctcc tacttaaaaa
     1201 ggaggaggaa ttaaaaggtg gtggttcttg ataaaaaatc aactattttt aaattgaatc
     1261 gtttattcag aactcatgtt attagcatat aaatgatact gacaaagttg ccttatttct
     1321 gttcggcatt aaagttgcat ttacataaaa acctatcttt acggatagaa ttgtgtcaat
     1381 ttgtctaaat ttatcgaaat gcttgagttt tccaatatta ttgaacaaga tgtagaaatg
     1441 attccgctgt tatctatcga agaagatgac aatataaaaa gcagcaaact tcctaaaata
     1501 ttacccatat tgcccttacg caataatgtg cttttcccgg gagtagtcat ccccatttct
     1561 gtcggcagag agaagtccat caaagccgtc aaaaaggcat ataaagaaaa taagtatatc
     1621 ggcgttattg cccagcgcga cccaaaagtg gaagaacccg aaccgggcga tttatacagt
     1681 gttggtacta cggccaaaat catccgcatt ctcaacatgc cggatggtgg taatactgtt
     1741 attttgcaag gagtacatcg ttttattgct aaaacactca tatcctcaga cccaatcata
     1801 cagactgaag ttgaatatct ggaagatgtg ccattatctg acaaaacgca agagaaagca
     1861 cttattgcct ctatgaaaga tatggcagga cgcattatcg aactttccaa ccatataccc
     1921 aatgaggcta aattcatctt gaataatatc aacggattga agtttttagc caactttatc
     1981 acctccaata ttccgattga gcttgcacaa aaacagcaga ttttagaaac caattccgtt
     2041 gaagaacggg tgcagcaggt actaaaatat ctgaatcagg aattacagtt gcaggaaata
     2101 aaagccaata tacaagagaa aacaaaaaca gatatagaca agcagcagcg tgattatttt
     2161 ctcaaccaac agatgcgtgc catacaggaa gaattgggtg gcgatatgta tcaggaggaa
     2221 attaacaggc tgaaagaaaa agcgaaagaa aaaaaatggt ctgaagctgt tgccaaacaa
     2281 tttgacaaag aaataggaaa attacagcgc atcaacccgg ctgctgccga atattccgtt
     2341 atattgaact acctggattt aatggtagag cttccctgga atgagattac aaaagataat
     2401 tttgatttga aaagagccgg aaaaattctg gatgaagatc attatgccct ggataaaatc
     2461 aaagaccgca tcattgaata cctggcagtt ctgaaattaa agggtgatat gaaatccccg
     2521 attttatgtt ttgtaggccc tcctggagtt gggaaaacat cattgggaaa aagtattgcc
     2581 cgttcattag acagaaaata tgtgcgtgtg gcattgggtg gtttacacga tgaaagtgaa
     2641 atacggggtc accgcaagac ctatatcggt gctatgccgg ggcgtattat ccagtctttg
     2701 aaaaaagtac aaacagcaaa tcccgttttc gtgctggatg aaattgataa agtgggaaaa
     2761 gaccatcgcg gcgatccgtc atctgccttg ctcgaagtgt tagaccctga acaaaatact
     2821 accttttacg ataactatct ggaacaagaa tacgacttgt caaaaatttt gttcattgca
     2881 acagctaata gcttaaacga gattccgcat gccttgcgcg acaggatgga agtaattcaa
     2941 ttgagtggct attctttaga ggaaaaaaca gaaattgcca aacgccattt ggtagcaaaa
     3001 caacgagaag ctcatggatt gaaagcatcg caaattaaat ttaatgataa agttatccag
     3061 tttattattc accattatac ttatgaaagt ggcgtgcgtg agttagacag aatgatagcc
     3121 tctatgatgc gcaatattgc caaatgcatt gcaacggatg aatcctataa tgtacaagta
     3181 acgacagaag atgtggctcg tattttgggc aaattcagat ataaccacga taagtatacg
     3241 caaaacaatc cttgcggggt tgcaatcgga ttagcatgga cacctcatgg cggtgatatc
     3301 ttgttttttg aatcgacagt aagcagagga aaaggcaatt ttaccttaac aggaaatttg
     3361 ggcaatgtca tgaaagaatc attcactacg gcatttacat atttgcaatc caaagcggat
     3421 agtttacaaa ttcctgtaaa ggcattttct gattggaatc tgcacgtaca tgtacctgaa
     3481 ggagccattc cgaaagacgg tccaagtgcc ggtattgcca tttacagtgc acttgcttct
     3541 ttgtttaccc aacgcaagct taagccgttt acggcactca gcggcgaaat taccttacgc
     3601 ggaaaagtac tgcctgtcgg cggcattaaa gaaaagatat tggctgctaa acgtgcggga
     3661 attaaagaaa ttattttgtg taaggaaaat cagaaagacg ttgacgatgt tccacaggat
     3721 tttgtaaaag gcttgaagtt tacttatgta gaagatatgg atgaagtgat tgaactagcc
     3781 ttggaaaaaa agaaaattcc agatgctatc cattttgaat aataatactt tttaggtcaa
     3841 ctgaaaattt ccgtaagtac ttttgtctgt ttaaaagaga atcaaatttg tggtttccta
     3901 taagataaat actcatttag agtaacacat tgtgcagttt aaaccacata aaaacatagc
     3961 atatttaaac ataaataatt gtctggtgca aatcagtaaa gatagccaag caaaaacata
     4021 gtatcaccat tggcgattcc tgtattttct attattacct caatttttgt atgctaaact
     4081 tttttaaaat aaaaaagctt atatatctat gtggtaaaaa aaattagatg atactgcaca
     4141 aaaaggttag tatatttttt tttcatgctt tatcgtatat ttacgataaa taaaatcagt
     4201 attatgcttt atcatgaatc tgactactac gaagaagata tacaacttgc tgaagtatgt
     4261 aaggcaattg cgcatcctgc acgtattcaa atcttgcgca tcatcgtggc aaaacaaaat
     4321 tgcacggtaa gtcatttggt gaaaaaaatg aatctggcac agtctacaat ttcgcaacat
     4381 ctcaaagcac taaaagacgc aggtataata aaaacaagca atctgggtgt gagtacagtg
     4441 ctttctgtca aaatcaaatc gcttaaaaat gcagaaaaag ccatgaaatt tttgacacag
     4501 gcattagaca aatagtcaat gtttaataca tattgctaaa tttatttcaa ctttcttata
     4561 gaagtagtaa ggaatgcctt tcagtaacta ttacaactta atcggtaatt aaccggttat
     4621 ataaaaatgc agctgttaag cttatcccag caacaatact ccataaaacg gtaaagccat
     4681 attgttcggc tatccacgaa cctccaaagg ggcctaaact gctggaaaaa gcccaggtca
     4741 ttgtccagat acccgcatat tgccctcgtg tagaatcggt agtgcgactc acccagtaat
     4801 taaacaacac cgaaaaactg aacatttctg ccagcgtaat aaagctcatc atcacccatg
     4861 ctccgaaaaa atctaaatga aatactaccg ctgtaaggta ggcgataaca tgcatcagta
     4921 cgcctatggt aacggcacgc tgcctcgacc agttcttttc tatccaaaat atgacagtca
     4981 tttcaa
//
LOCUS       DAOQII010000191         4979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_9947_length_4979_cov_5.174045, whole genome shotgun sequence.
ACCESSION   DAOQII010000191 DAOQII010000000
VERSION     DAOQII010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9947_length_4979_cov_5.174045"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            596..1183
                     /locus_tag="PLF48_08955"
     CDS             596..1183
                     /locus_tag="PLF48_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49495.1"
                     /translation="MLKLITPFILVLNVNFLYSQFCTGKINCFDKLGNKYGYWKEKRV
                     FPYDSLNKMYIRSNAGIIIDGKYQVYTTAEGNYINNIKLPGWIYYSHHEFPCEGYMFD
                     ITEVYPDGSFNVKKYDSEILYDKDSNIIFGEIYNLLDTIDIYCYKNNCDFCYKNETIK
                     EVYSDNYNRIFFEILMFANGHRKYKRTNPSKKNWQ"
     gene            1319..1801
                     /locus_tag="PLF48_08960"
     CDS             1319..1801
                     /locus_tag="PLF48_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49496.1"
                     /translation="MEIKGVPVFQLKKLIIPEQWKVVKNDFVDLEPDNSYPIDQVFYH
                     FDEDILNVTYNDWCIDLGFYGGYLDNNRSGFFKIVVFKGDFNDGEFFEFFISRSTDKI
                     VDKLNSYLKIIPTGQLENLSGVKYDDEYDFDTFHIYSAIENIDYRLSNDELEEIAKAK
                     "
     gene            complement(2042..3583)
                     /locus_tag="PLF48_08965"
     CDS             complement(2042..3583)
                     /locus_tag="PLF48_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HNF49497.1"
                     /translation="MKLEIENVELQELHFDDYHDLKEIMIEAYPVLDEAYWKKHQIKT
                     LIDKFPEGQVVVKVNGQLAGCALSIIVDYAKYGDKHTYKEITGNYTFNTHDDDGDILY
                     GIDLFVKKEFRGLRLGRRLYDYRKELCEQHNLKGIVFGGRIPNYHTHAKKLSPKEYIE
                     HVKRKEIDDPVLNFQVSNDFHPSKILKGYLEGDSASNEYAVLLKWDNIYYEKPTEKII
                     QNKTSVRVGLVQWQMRPYKDLDELMQHAEYFLDAVSGYRSDFALFPEFFNAPLMAEYN
                     HLSEPEAIRKLALYTDEIVDRFSALSVSYNINIITGSMPEIKDGLLYNVGYLCQRDGS
                     VDRYVKLHVTPDEAKVWGMQGGNELKIFDTDCGKIGVLICYDSEFPELSRMLADEGMD
                     ILFVPFLTDTQNGFSRVKICAHARAVENECYVVTAGSVGNLPNVHNMDIQYAQSMVFT
                     PCDFAFPTNGIKAEATPNTEMILIADLEIDLLRELHKFGSVRNLKDRRKDLFNIKKTS
                     NLITN"
     assembly_gap    3797..3896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3974..4876
                     /locus_tag="PLF48_08970"
     CDS             3974..4876
                     /locus_tag="PLF48_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNF49498.1"
                     /translation="MKSLMNVKTIPFLWMLSILLLTQCRSKREGVAVESAEPAAVSVG
                     SVAKVDYATTETDASVATDLAAPPPPIPPAAPTVDKSISDSIGKKIIKDGTITWETGN
                     LKSTNAWLKKLASQHQAYIANESSSSNDYRTDYSMEVRLPAEQFDQFINALENEVSDF
                     DTKNITAQDVTEEYIDVYARIKTKKELEQRYFAILNKANKVDDILKTEEYLNNVRNEI
                     ESAEGRMRYLNDRINLSTLHLNFYQTSKVSHGFGRDIFESFSDGWKGILNFILVLIRL
                     WPILLIIGAIVFWFIRRRKNRLIT"
BASE COUNT         1660 a          808 c          825 g         1586 t
ORIGIN      
        1 catatgagca tggatattga acatcgttta aatgattaaa aactacacat aacaagaaaa
       61 ttagttacag ttttggtaga tttggtaaaa aaacaatatt tgtattatat ttgaatttat
      121 attatcgtta ggtgttctca cctaacgaaa gtatctatta atatgttgag ctgtttaaac
      181 cacatagaag catagcattt ttaatctcaa gtagttgttt ggtgcatgtt agtttagaaa
      241 gtcaagcaaa acatagtatt gcttttggcg atttctgcat tttcaatttt atctcaattt
      301 aaatcatcgt tagttgcttt acctaatgta agtatctttt aattccattt gtttaagctg
      361 tactttctac ctgagtttgg gataagcatt tcagtttaaa aattgttttg caggtgttgg
      421 ataaataaaa ttcaaaaacc aaaaaaatga gattatacaa aaatgctatg cgaattttct
      481 aatcttattg ttaatatcaa tattataaat gtttttaata aaaacgaata aaaaaaagta
      541 aaaagttgtt tggacgaata aaaatagtgt atatttgggt agaagcagac attttatgct
      601 gaaactaatt acaccattta ttctagtact taatgtgaac tttctttatt ctcaattttg
      661 tacaggtaaa ataaactgtt ttgataaatt aggcaataaa tatggttatt ggaaagaaaa
      721 aagagtattc ccatatgata gtttaaataa aatgtatatt agaagtaacg caggaataat
      781 aatagatgga aaatatcagg tatatacgac tgcagaagga aattatataa acaatattaa
      841 attaccaggt tggatatatt attctcatca tgaatttcca tgtgaaggtt atatgttcga
      901 tatcacagag gtatatcctg atggttcatt taatgttaaa aaatacgatt ctgaaatttt
      961 atatgacaaa gatagcaaca taatatttgg tgaaatatac aatctattag atactattga
     1021 tatatactgt tataaaaata actgtgattt ctgttacaag aatgaaacca ttaaagaggt
     1081 atattcagat aactataata gaatattttt tgaaatatta atgtttgcaa atggacatag
     1141 aaaatacaaa agaacgaacc ctagcaaaaa aaattggcaa tagttgtggt tgatttggta
     1201 attaaagttt tgagtaataa atttaatttg taaataagtg ttggttggtg ctaaacatgc
     1261 acaaccatcg ccaattttca accgttataa gcaaagccag catgaacaat agaacaaaat
     1321 ggaaataaaa ggtgtcccag tttttcagct taaaaagctt ataattccag agcaatggaa
     1381 agtagtaaaa aatgactttg tcgaccttga gccagataat agttatccta ttgaccaagt
     1441 cttttatcac tttgatgaag atatacttaa tgttacttat aatgactggt gtattgactt
     1501 aggtttttat ggtggatatt tagacaataa cagaagcgga ttttttaaaa tagttgtttt
     1561 taagggagat ttcaacgatg gagaattttt tgaatttttc atttctaggt caactgataa
     1621 aatagtggat aaattaaact cctatttgaa aattatacca acaggacaat tagagaatct
     1681 ttccggagtg aaatacgatg atgaatatga ctttgacact tttcacattt attcagcgat
     1741 agaaaatatt gattacagat tatctaatga cgaactggaa gaaattgcaa aagcaaagta
     1801 agacctgtaa aaatggctct gcttataacg cgggtagcca ttcctcccaa gtccgacagg
     1861 tgcacaactt cactttacta aaggttattt tttaaaatat aataaaatat atttgcaatt
     1921 atagaaacta ttccatttta gccatttatt tcttttgcat actgtggagt agttcagctg
     1981 ataaactaaa aaaaactatg catctatgtg gtgaaaatta taaatcaaac cgctcaggtg
     2041 cttaattcgt aattaaattc gatgtttttt taatattgaa caaatctttt ctgcggtctt
     2101 tcaaattgcg cacactgccg aatttatgca attctctaag caagtctatt tccaaatcag
     2161 caattaaaat catttccgta ttcggggtgg cttccgcctt aatgccattt gtaggaaagg
     2221 caaaatcgca aggggtaaac accatagatt gcgcatattg aatatccata ttgtgtacat
     2281 ttggcaaatt gcccaccgag ccagcagtta ccacgtagca ttcgttttct acagccctgg
     2341 catgcgcaca tatttttacc ctcgaaaaac cgttttgcgt atccgtaagg aatgggacaa
     2401 acaagatgtc cataccttca tcggcaagca ttctgctcaa ctcaggaaat tcggaatcat
     2461 aacaaatcaa tacgccaatt ttgccgcagt cggtatcaaa tattttcagt tcattaccac
     2521 cttgcatacc ccatactttt gcttcatcag gcgttacatg cagttttaca tatcgatcta
     2581 cagagccatc gcgttggcat aaatagccaa cattatacaa taatccgtct ttaatttccg
     2641 gcatacttcc cgtgatgata ttgatattgt aactgactga aagtgcagaa aagcgatcta
     2701 cgatttcatc cgtgtataag gcaagtttcc gtatggcttc cggctctgat aagtggttat
     2761 attcagccat cagcggtgca ttgaaaaact cgggaaacaa agcaaaatct gaacgataac
     2821 ccgaaacagc atccaggaaa tattcggcat gttgcatcaa ttcatccaag tctttataag
     2881 gccgcatttg ccattgcact aagcctactc tgacagatgt tttgttttgg ataatttttt
     2941 ctgtcggctt ttcataataa atattatccc atttcaaaag tacggcgtat tcattcgatg
     3001 cagaatcgcc ttctaaatag ccttttaata tcttggacgg atggaaatca ttggacacct
     3061 ggaaattcaa aacaggatca tcaatttctt ttcgttttac atgttcgata tattctttgg
     3121 gcgaaagttt ttttgcatgt gtatgataat tcggaattct gccaccaaaa acaatacctt
     3181 tcagattatg ttgctcacac aattcttttc ggtaatcata cagcctcctt ccgaggcgca
     3241 atccgcgaaa ttcttttttg acaaacaaat caatgccgta taaaatatct ccatcatcat
     3301 catgggtatt gaacgtgtaa tttccagtga tttctttata cgtatgcttg tcgccatatt
     3361 ttgcataatc tacaataatt gacagcgcac atcctgctaa ctgcccgttt acttttacta
     3421 cgacttgccc ttcgggaaat ttatctatca gcgttttgat ttgatgtttt ttccagtaag
     3481 cttcatccaa aacaggataa gcctctatca tgatttcttt taaatcgtga taatcatcaa
     3541 aatgaagttc ctgcaattcg acattttcaa tttctaattt catatctgtg tgggttgaaa
     3601 ttttagactg caaaattacg gaggatgcac tttttataca aattatcgaa ttgttgtaag
     3661 agaaataaat tcatgatagt tatcatcatt tcaggcgtaa atacgtcaat taccatccga
     3721 ttttatttaa ggagctgtag aataacaaac attaacaata cacgacttta caaattccaa
     3781 aactttgtgc aactttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnataa
     3901 caaacattaa caatacacga ctttacaaat tccaaaactt tgtgcaactt tattgcaatt
     3961 aaaccaacca gtcatgaaat cattaatgaa tgttaagacc atcccgtttt tgtggatgct
     4021 aagtatttta ttgcttacgc aatgtagaag taagcgtgaa ggagttgctg tagaatctgc
     4081 tgaacctgct gccgtgtctg taggcagtgt ggcaaaagta gattatgcaa ctactgaaac
     4141 tgatgcttcc gtagcaactg acttagccgc ccccccacct cctatacctc cggcggctcc
     4201 tactgtggat aaaagtatat cagacagtat tggaaaaaaa attatcaaag atggcacgat
     4261 tacctgggaa accggaaatc tgaaatcgac gaatgcatgg ctaaaaaaat tggcatcaca
     4321 acatcaggca tatattgcaa atgaatcttc aagttctaat gattatcgca cagattactc
     4381 catggaagta cgattgccgg ctgagcagtt tgaccaattt attaatgctt tggaaaacga
     4441 agtcagcgat tttgacacta aaaatattac ggcgcaggat gttacggaag aatatattga
     4501 tgtttatgcc aggataaaaa caaagaagga actggagcag cgctattttg ccatattgaa
     4561 caaagccaac aaagtagatg atattttaaa aaccgaagaa tacctgaata atgtgcgcaa
     4621 tgaaattgaa agtgctgaag gcagaatgcg ttatctgaat gacaggataa atttatcgac
     4681 actgcactta aacttttatc agacttcaaa agtatctcac ggttttggaa gagatatttt
     4741 tgaaagtttt tcagacggat ggaaaggcat actaaacttt atattagtct tgattcggct
     4801 atggccaatt ttactgatta tcggtgccat tgttttttgg tttatcagac gccgaaaaaa
     4861 tcgattaata acctgagttc gggataaaaa tagttaattt tttatataga actacctcct
     4921 tataattcct ccaccttgtt aaagtggagg aagaaatgag aaatcaacta ttttatccc
//
LOCUS       DAOQII010000192         4956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10032_length_4956_cov_5.650275, whole genome shotgun sequence.
ACCESSION   DAOQII010000192 DAOQII010000000
VERSION     DAOQII010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10032_length_4956_cov_5.650275"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="PLF48_08975"
     CDS             <1..1477
                     /locus_tag="PLF48_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNF49499.1"
                     /translation="CIYVFFPSRQLKKLRFKNKYGHILIILLFYIILTVVFVYFIHLF
                     EKINYLKYGVKGSIMNLTKFDTLKWLIVFIFTGYEDEIFPISTGAKIMTTLSFFLAPA
                     TLIITFAWQYLTNNRLKKISMGEIKILDKDHDVICGWNMKTADLIEKLYNQNSASGFE
                     NRKIVLLNEQLKCIDNLSENIRVLIERDIVRYVNGDPTDIEMLKGIRIQDAHSVIIVN
                     QGYEKIHDENVLLIAYAISTYCKELKGNHLSNIYMMAEITNPVYINKLKAADVNVVIC
                     SGDLINSLMIQSIHCEGIYNPIKSLIDYGEGNDFYMIHCKDYPVTLDKNYDELLMLLR
                     KFRIQLIGIKTNFLNEVKQVIIDDDLIKLKLREKRIANMREVITDSLLSYLPAAIQER
                     LALELVDLCETNKLNDLSNIDEAIVQKSIINNLPDDLKKLIPIKFSQYIRDATGFSSQ
                     YLINPQCKTECAYKTQNEDELILLALDNKSLNMISASNLKG"
     gene            1531..2811
                     /gene="purD"
                     /locus_tag="PLF48_08980"
     CDS             1531..2811
                     /gene="purD"
                     /locus_tag="PLF48_08980"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNF49500.1"
                     /translation="MRILLLGSGGREHAFAYKLVQSPLCSRLFIAPGNPGISALGTCV
                     PISATDFPAIKEFVLTNQIEFVIVGPEAPLVEGIYDYFKNDTQLRSIPVFGPSKMGAL
                     LEGSKAYSKAFMQRHQIPTAKYKEFTTDNFEEGVHYIQQQQTPIVLKADGLAAGKGVL
                     ICNTHAEAQQEFEAMLGGKFGDAGNKVVIEEFMDGIEFSVFVLTNGKEYKILPSAKDY
                     KRIGEGDTGLNTGGMGAVSPVPFVTNEMMQYVEKEIIQPTIQGLAAENIDYFGFIYVG
                     LMWLKTREIKVVEYNCRMGDPETEAVFPRIKSDLVELFQSTVNHQLSTAEIEIDSRSA
                     VTIILASGGYPEDFVKGKEISGIEAADENAIIFHCGTKLDDGKLLTNGGRVLAVTALA
                     ADLQEALTSANGIAEKIQFDGKYFRCDIGYEFKK"
     gene            2867..4951
                     /locus_tag="PLF48_08985"
     CDS             2867..4951
                     /locus_tag="PLF48_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49501.1"
                     /translation="MGKKKAHINPPVVKKSETVQLQKKEWKPLAEKWFYILLAAFSFL
                     LYINTINHKYVLDDDMALGKNKIVTKGISAIPEIMTTAYRAGAWQREEGLYRPLSLVF
                     FAVEWQIAPDKPFIGHLVNILLYVLTALVLFRLLRKWFSTYTIFIPFLISMLYIVHPL
                     HTEVIANIKSRDELLGALFAFLSLYAFHDYAITNSIKKISLGGLFFLLSVLSKESSLT
                     LFAVIPLSIYFFSPNTSKKTYLYTMATLVALVVLYFLMRKMALGNLVNFKSIDLYNNS
                     IVATDNKLYRFATAIVYLGFYLWKFILPHPLAFDYSYNTIPFATPGDWRFLLSFTILI
                     AMGAYAIYTIKKRDSIGFGFLFFILTISLVSNLVILIEATTADRFMYLPSLGCCIVFI
                     FLLEKLFHFSTVQLPNFAYFFSQKKIAAVILAIILVLFSIKTFTRNLDWKDNFTLFTK
                     DKDNNPKSYRTIGAYANELYNVRIKDLPDYAPEKQALCLEMMDYAKRSLAILSDNFKM
                     WTLLAFGYTQLKDYPQVIQVFEEGKKHYNNDIDWDKFHIMGIDAYYKTQQFQEALTVC
                     DTALKTMPDNAVLWNSRGMVLLDLNRREESMSDLQHALKLDSTVSSAWYNLGNWYARG
                     NDFTRAITYYEKSVSLDSTQLQAYNNMGNCYAVTQQFDKAILQYKKVLSLQPDNPDAL
                     RNLAIAEKQIGK"
BASE COUNT         1653 a          843 c          894 g         1566 t
ORIGIN      
        1 ttgcatatat gtgtttttcc cctcaaggca gttaaaaaaa ttacggttta aaaacaaata
       61 tggacatata ctaataatac ttttatttta tattatatta actgtcgtat ttgtttactt
      121 tatacatcta tttgaaaaaa taaattattt aaaatatggt gttaaaggaa gtataatgaa
      181 tcttactaaa tttgacacat taaaatggtt aattgtcttt atttttacgg gctatgaaga
      241 cgaaattttt ccaataagta ccggagcaaa aattatgaca accttgtcgt tcttcttagc
      301 acctgcgaca ctgataatta cttttgcttg gcaatattta acaaacaata gattaaagaa
      361 aataagtatg ggtgaaatta aaatattaga taaagaccat gatgtaattt gtggatggaa
      421 catgaagaca gctgatttga ttgaaaaatt atacaatcag aattcagcat ctggatttga
      481 aaatcgaaaa atagttctgc tgaatgaaca gctaaaatgt atagataatc taagtgaaaa
      541 cattagggtt ttaatagaaa gagatattgt aagatatgta aatggtgatc ctactgatat
      601 tgaaatgctt aaaggcatac gtattcaaga tgcacactca gtaataatag ttaatcaagg
      661 atatgaaaaa atacatgacg aaaatgtatt gctaattgcc tatgcaattt caacctattg
      721 taaggaactt aaaggaaatc atctgtcaaa tatatatatg atggcagaaa ttacaaatcc
      781 tgtttatatc aataaactaa aagctgcgga cgtaaatgta gtaatatgct ctggagattt
      841 aattaacagc ctgatgatac aaagtattca ttgtgaagga atatataatc caataaaatc
      901 tttaattgat tatggagaag ggaatgattt ttatatgata cactgcaaag attatcctgt
      961 tactcttgat aaaaattatg atgagttatt gatgttatta agaaaattca gaatacaact
     1021 tataggaatt aaaacaaatt ttttaaacga agttaaacaa gttatcattg acgatgattt
     1081 aattaagctc aaattgcgtg aaaaaaggat tgcaaacatg agagaagtga ttactgatag
     1141 cttattatct tacttacctg cggctataca agaacggctc gctcttgaat tagttgattt
     1201 atgtgaaaca aataaattaa atgatttatc taatattgac gaagctattg tgcagaagtc
     1261 aattattaat aatttgccgg atgatttaaa gaaattaatc cccattaaat tttcacagta
     1321 tataagagat gcaaccggtt tctcatctca atatcttata aacccccaat gtaaaacaga
     1381 gtgtgcatat aaaacgcaaa atgaagatga attaatttta ttagcccttg ataataaaag
     1441 cctgaatatg atttcagctt ctaatcttaa aggataagaa atttttcgac taattgtttt
     1501 tcttgaatta cttttgcaaa aaaatcaact atgcgtattc ttcttttagg cagtggcggc
     1561 agagaacatg cttttgctta taaattagtt caaagtcctc tttgctctcg cttatttatc
     1621 gctccgggta accccggcat atcagcactg ggtacttgcg ttccgatatc tgctacggac
     1681 ttcccggcaa taaaagaatt tgttttgact aatcaaattg agtttgtaat cgttggtcca
     1741 gaagctccat tggtggaagg catttatgac tatttcaaaa atgatactca acttcgttcg
     1801 attccggtat ttggtccttc taaaatgggt gcacttttgg aaggaagcaa agcttattca
     1861 aaagcattta tgcaacgcca tcaaatcccc acagcaaaat ataaagagtt taccactgat
     1921 aatttcgaag aaggagtgca ttatattcaa caacagcaga cacccattgt tttaaaagct
     1981 gatggattag cagccgggaa aggtgtactg atttgcaata ctcacgcgga agcgcaacaa
     2041 gaatttgagg ctatgctggg aggcaaattt ggcgatgccg gaaacaaagt agttatcgaa
     2101 gaatttatgg atggtattga gttttctgta tttgtactga cgaatggaaa agaatataaa
     2161 atactgccat cagccaaaga ttacaagcgc atcggcgagg gcgacaccgg gctgaatacg
     2221 ggtggcatgg gtgccgtctc tcctgttcca tttgtaacta atgaaatgat gcaatacgtg
     2281 gaaaaagaaa tcatacaacc tactattcag ggtttggctg ctgaaaacat agactatttc
     2341 gggttcattt atgttggatt gatgtggctg aaaaccagag aaataaaagt agtggaatac
     2401 aactgccgta tgggagatcc ggaaacagaa gccgtttttc ccagaataaa atcagatttg
     2461 gtcgagcttt ttcagtcaac tgtcaaccat caactgtcaa cggctgagat tgaaattgac
     2521 agtcgttcgg ctgtaactat tattttggcc agtggcggct atcccgaaga ttttgtaaaa
     2581 ggaaaagaga tttccggaat tgaagcagct gatgaaaatg ctattatctt tcactgtgga
     2641 acaaaattgg atgacgggaa attgctgaca aatggcggac gagttcttgc tgttactgca
     2701 ttagcagctg acttgcaaga agcactgact tcggcaaacg gcattgctga aaaaatacaa
     2761 tttgacggca agtattttcg ctgtgatatc gggtacgagt ttaaaaaata agtatcaata
     2821 aaactgctga tatttaaata cctttaccta atcttatttt cttagaatgg gcaaaaaaaa
     2881 agcgcatatt aatcctccgg ttgtaaaaaa atcagaaact gttcaattgc aaaaaaaaga
     2941 atggaaacca ttagccgaaa aatggtttta tatacttctg gctgcgttca gttttttatt
     3001 atacatcaac accatcaacc ataaatatgt actggatgat gacatggctt taggcaaaaa
     3061 taaaatcgta accaaaggca tctctgctat tcccgaaatt atgactacgg cataccgtgc
     3121 cggtgcatgg cagagagaag aaggtttata tcgcccttta tcattagtgt tttttgccgt
     3181 agagtggcaa atcgctcctg acaaaccatt catcgggcat cttgtaaata tactgttata
     3241 cgtgttaacg gcacttgtgt tatttcgttt actcagaaaa tggttttcaa cctacactat
     3301 ctttatcccg tttcttatct cgatgctcta tattgtacat cccttgcata cagaagtaat
     3361 tgccaatatc aagagccgcg atgaactatt aggtgcactc tttgcatttt tatctttata
     3421 cgcttttcac gattatgcga ttacaaattc aatcaaaaaa ataagcttag gcggtctgtt
     3481 ttttttgttg tctgtattgt caaaagaaag ctcgcttaca ctatttgccg tcattcctct
     3541 aagtatttat tttttcagtc caaatacttc aaaaaaaaca tatctctaca cgatggcaac
     3601 actcgttgcc ttggttgttc tttatttcct gatgcgaaaa atggcacttg gcaatctggt
     3661 taatttcaaa tccattgatt tatataacaa ctcaattgtg gctaccgata ataaactata
     3721 ccgctttgct actgccatcg tatacctcgg tttttatctg tggaaattta tcctgcctca
     3781 tccacttgca ttcgattatt cttataatac catccctttt gcaacacccg gcgactggcg
     3841 ttttttatta agttttacaa tattgattgc tatgggagct tatgccattt acaccattaa
     3901 aaaaagagac agcatcggtt tcggtttcct atttttcatc ctcaccattt cattagtaag
     3961 caatttagtc atcttgattg aagccactac agccgaccgt tttatgtatt taccttcttt
     4021 aggttgctgt attgttttta tctttttatt ggaaaaactt tttcatttct caacggtaca
     4081 gcttcccaac tttgcttact ttttttcgca gaaaaaaata gccgctgtaa tacttgcaat
     4141 cattctagtg ctgttttcca taaaaacatt tacacgtaat ttagactgga aagataattt
     4201 tactttattt accaaagaca aagataataa tcccaaaagc tacagaacca ttggtgctta
     4261 tgccaatgaa ttatataatg tgcgcataaa agatttgccc gactatgcac ccgaaaaaca
     4321 agctctttgt ttggaaatga tggactatgc caaacgaagc ttagccatct tgtccgacaa
     4381 tttcaagatg tggacgctcc ttgcttttgg ttatacgcag ttaaaggatt atccgcaagt
     4441 gatacaagtc tttgaagaag gcaaaaaaca ttataataat gatatcgact gggacaaatt
     4501 tcatattatg gggattgatg cttattacaa aacacaacaa tttcaggaag cattaactgt
     4561 atgtgatact gcattgaaaa caatgccgga taatgccgtt ttgtggaaca gccgaggtat
     4621 ggttttattg gatttaaaca gaagggaaga atcaatgagc gacttgcaac atgcactaaa
     4681 attagacagc acagtaagct cagcctggta caatctgggc aactggtatg cccgcggaaa
     4741 tgattttacc cgagctatta cttattacga aaagtcagtt tctttagaca gcacacaatt
     4801 gcaggcttat aacaatatgg gtaattgtta tgccgttaca caacaatttg acaaggctat
     4861 tttacagtat aaaaaagtac tttcgcttca gcccgacaat ccggatgctt tgcgaaattt
     4921 agccattgcc gaaaaacaaa tcggaaaata agtgca
//
LOCUS       DAOQII010000193         4901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10183_length_4901_cov_4.876187, whole genome shotgun sequence.
ACCESSION   DAOQII010000193 DAOQII010000000
VERSION     DAOQII010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10183_length_4901_cov_4.876187"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLF48_08990"
     CDS             complement(<1..328)
                     /locus_tag="PLF48_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF49502.1"
                     /translation="MEKIPCLDLKKQNQQVKAEIFELFDKVFENTAFSGGSFVEEFEQ
                     QFASYCQTNYAIGVSNGTTALHLAMLALGIGQGDEVIIPANTFIATVWGVSHAGATPV
                     FVDCTAD"
     gene            complement(371..859)
                     /locus_tag="PLF48_08995"
     CDS             complement(371..859)
                     /locus_tag="PLF48_08995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF49503.1"
                     /translation="MELISENSPKRSLNEVAVGSNVKIFDFVNAYKCTIDDNSKVGAF
                     VEIQKGATIGKNCKISSHTFICEGVHIADNCFIGHGVMFTNDLFPRATNPDGSAQTDA
                     DWELIETYVQKGASVGSNATILCGITIGENALVGAGAVVTKDVPANTVVAGNPAKVIK
                     QI"
     gene            complement(861..1865)
                     /locus_tag="PLF48_09000"
     CDS             complement(861..1865)
                     /locus_tag="PLF48_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF49504.1"
                     /translation="MINIALIGYGYWGPNLLRNFSSNPNCTVKVVADFKSERLDLAKK
                     FLPGITTTQQIDEVFSDSSIDAVVIATPVFTHFGLAKKALDNDKHVLIEKPMTSTVEE
                     ASALIELANKRNKLLMVDHTFLYTGAVQKMKSMIASDEIGKVKYFDSTRINLGLFQPD
                     VNVLWDLAPHDISILTYLINDKPLSVQATGISHTHNDIENIAYLTVNYASDFIAHFNC
                     SWTSPVKIRMMLIGGDKKMLVFNDLEPTEKIKVYDTGYSHKTDEEKQRILVDYRAGDI
                     FIPKIETKEALSGMAADFINCIINGKTPIASAASGLEVIKILEASQASIKNKGKEIII
                     "
     gene            complement(1995..2336)
                     /locus_tag="PLF48_09005"
     CDS             complement(1995..2336)
                     /locus_tag="PLF48_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF49505.1"
                     /translation="MSAIRNQVQLIGNLGKKPEIKSYGDDKKFAVFSLATSDYYVDQK
                     GEKQQQTQWHNIVVWQKNLVTVAEKYLDKGMEIVLSGKLQYREYNDKDGIKRTVTEIS
                     ADEILMLTKKN"
     gene            complement(2458..3207)
                     /locus_tag="PLF48_09010"
     CDS             complement(2458..3207)
                     /locus_tag="PLF48_09010"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF49506.1"
                     /translation="MLLENQIIYRNRRLLKGYRFTSIFPYDEYFHEFKINQDKFLINA
                     LHLKAEQPKGLIFFMHGSFKHLQFHIPLCKEFLLNDFDVCLFDYPKYGKSRGKLHEHL
                     LYKIADKILYLTLKNERAEEIKYKRLILCGRSLGTAIAAHTALHTQAERLLLMTPYFN
                     MKELIQLHLKRNDLPRLTQVFPNNQYLPKVQMPVTILHGDKDKLIPLAFAQRLTPLLK
                     TNDEFVIIKDANHFNLHQQDQYKKHIKKLLS"
     gene            complement(3224..3814)
                     /locus_tag="PLF48_09015"
     CDS             complement(3224..3814)
                     /locus_tag="PLF48_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNF49507.1"
                     /translation="MKNFNKYFLLLSLVIFSLNACKANEGQKNTTSTTAQSIYDFTLN
                     DIDGKPVSLSAYKGKVVVIVNTASQCGLVGQLGEIQKFYEKYKDKGVVVLGFPANNFL
                     GQEPLSNADIKSFCSKNYGVTFPMFEKISVKGDDMHPLYKYLTEKAENGITDAPVKWN
                     YQKFIIDKNGHVVCAISPRTTVNDQEFIQNIDDLLK"
     gene            4033..4704
                     /locus_tag="PLF48_09020"
     CDS             4033..4704
                     /locus_tag="PLF48_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF49508.1"
                     /translation="MKIAVLTNDKQSLETLNKIISKEHYKLYELKQHKDAIKLLKADK
                     PACIITDVELGDLDGIELCSELKSNDELKGIPVVFYADKADDYTQIAAFQSGAEEFIA
                     KSHKSKLIEARLKLIFKRFFDKDSIEKNIRTFGNIVIDEEQMVITKKGDPLKVSKKEF
                     QLIALLTSKPGKVFKRNNILLKVWGDDIIVGDRNIDTHIKKIRKKLGKNHIETIRGIG
                     YKFIP"
BASE COUNT         1557 a          893 c          779 g         1672 t
ORIGIN      
        1 tatcagctgt acaatctaca aaaaccggag ttgctccggc atgagaaacg ccccagactg
       61 tggctataaa tgtatttgca ggaatgatga cttcgtctcc ttgtccgatg cccaatgcaa
      121 gcattgccag atgcaatgcg gtagttccat tactaacacc aatggcataa ttcgtctggc
      181 aatatgatgc aaactgttgc tcaaactcct caacgaaaga acctcccgaa aaagcagtat
      241 tctcaaatac tttatcaaac aactcaaata tttcagcttt aacttgctga ttttgctttt
      301 ttaaatctaa acaaggtatc ttttccataa atcaatgagt aaaaataaga aaaaaaatcg
      361 agagctcgac ttatatctgt ttgataactt tagccggatt tccagctact accgtatttg
      421 ccggaacatc tttggttacc acagctcctg cgcctaccaa tgcattttcg ccgatagtaa
      481 tgccacacaa aatagttgca ttactcccga cagacgcacc tttttgtaca taagtctcaa
      541 taagctccca atctgcatcc gtttgtgctg aaccatctgg attagttgct cgtggaaaca
      601 aatcattcgt aaacattaca ccatgcccaa taaagcagtt atctgcaata tgcacacctt
      661 cacatataaa agtatgtgaa gatattttac agttttttcc aatcgtagca cctttttgga
      721 tttctacaaa tgcgcctact ttagaattat catctatcgt acatttatag gcattgacaa
      781 aatcaaatat cttgacattg ctgcccacag ctacttcgtt taacgacctt ttgggtgaat
      841 tttctgaaat aagttccatt ttaaatgatt atttcttttc ccttattttt aattgatgcc
      901 tgagatgctt ctaatatttt tataacctct aaccctgatg ctgcagaggc tatcggtgtt
      961 tttccgttga taatacaatt tataaaatct gctgccattc ctgaaagtgc ttctttggtt
     1021 tcaattttag gaataaaaat atcccctgct ctatagtcaa ctaagattct ttgtttctct
     1081 tcgtccgttt tatggctata gcccgtatcg tacactttaa ttttttcagt aggttctaaa
     1141 tcattaaata ccagcatttt tttatcgcca cctatcagca tcatcctgat ttttacaggt
     1201 gatgtccagg aacagttaaa atgtgcaata aaatcagatg cataatttac ggttaggtag
     1261 gctatatttt caatgtcgtt atgtgtatgt gaaatgcctg ttgcttgtac actaagcggt
     1321 ttgtcgttta ttaaataggt taggatagaa atatcatgcg gtgctaaatc ccataataca
     1381 tttacatccg gctgaaacag ccctaaatta atacgtgtag aatcaaaata ctttacttta
     1441 ccaatctcat cagatgctat catagacttc attttttgta ctgctccggt gtacaaaaaa
     1501 gtgtgatcaa ccattaaaag tttatttctt ttatttgcaa gttctatcaa cgctgaagcc
     1561 tcttcgactg tagaagtcat tggtttttct atcaatacat gcttatcgtt atccagggct
     1621 ttctttgcca agccaaaatg tgtaaaaaca ggtgttgcaa ttaccaccgc atcaatagaa
     1681 gaatcggaaa aaacctcatc aatttgttgt gttgtagtga tgccgggcaa aaacttttta
     1741 gccaagtcca aacgttcaga cttaaaatct gccacaacct ttactgtaca attgggattg
     1801 gaagagaaat tcctaagtaa gtttggtccc caataaccat aaccaatcaa tgctatattt
     1861 atcacctttc aaaaataata aaaaatgaca aaaataactt atcctccatc ttggcttgtc
     1921 cacattaatg aaaatataaa aaaagccgct caaatgaacg gcttttaaaa ttggaagttt
     1981 gatttttatc taaatcagtt cttcttggtc aacatcaata tttcgtctgc actaatttct
     2041 gttaccgttc ttttaattcc gtctttatcg ttatactcgc ggtattgcag cttgcctgat
     2101 aaaacgattt ccatgccttt atcaaggtat ttttcagcta ctgttaccag atttttttgc
     2161 caaactacaa tattgtgcca ttgtgtttgt tgttgttttt ctcctttctg atctacataa
     2221 tagtctgaag tggctaaact gaatacggca aactttttgt cgtccccata agatttgatt
     2281 tccggttttt ttcctaagtt tccaataagc tgtacttggt ttctaattgc tgacataaca
     2341 tttcattttt ttgtttaaaa atttgtttcg actatataga gtacgattgc atacaatttc
     2401 cataaaaaaa atgaaaaaaa aatagtacaa attcataagc cacacaaaac caaccagtta
     2461 acttaacaac ttttttatgt gttttttata ttggtcttgt tgatgcagat taaaatggtt
     2521 ggcatcttta ataattacga attcatcatt agtttttaag agtggcgtta aacgctgagc
     2581 aaaggctaag ggaattaatt tgtccttatc gccgtgcaaa atagtcaccg gcatttgtac
     2641 tttaggcaaa tactgattgt ttggaaaaac ttgtgtcagt ctgggcaggt catttctttt
     2701 taaatgcaat tgtattaact ctttcatatt aaaatacgga gtcatcagca ataatctctc
     2761 tgcttgcgtg tgtaaggcag tatgtgctgc aattgccgta cccaaagatc gaccacataa
     2821 aattaatcgc ttatacttaa tttcctccgc ccgttcgttt ttcagtgtca agtaaagtat
     2881 tttatctgca attttatata acaagtgttc gtgcaatttg cctctgcttt tgccatattt
     2941 cgggtaatca aacaaacaca catcaaaatc attaagcaga aattctttgc ataaaggtat
     3001 gtgaaattgc aaatgcttga atgacccgtg cataaaaaag atcaggcctt tgggttgttc
     3061 tgcttttaag tgtaaagcat taatcagaaa cttgtcctga ttaattttaa attcatgaaa
     3121 atactcgtca taggggaaaa tggacgtaaa acgatatccc ttgagtaatc tgcgattccg
     3181 gtaaataatt tggttttcta aaagcacagc tcaaacatgg aatttatttc aataaatcat
     3241 caatgttttg tatgaactct tgatcattta cagtagttct gggtgaaatt gcacaaacca
     3301 catgaccatt tttatcaatg ataaattttt gataattcca ttttacaggc gcatcagtaa
     3361 tgccattttc agctttttca gtcagatatt tataaagcgg atgcatgtca tcccctttta
     3421 cagatatttt ttcaaacata ggaaatgtta cgccatagtt tttagaacaa aatgatttaa
     3481 tgtctgcatt acttaatggc tcttgtccta aaaaattatt ggcaggaaat cccagcacta
     3541 caacgccttt atctttgtat ttttcataaa acttctgaat ttcacccaat tgccccacta
     3601 aaccacattg gcttgctgta tttacgatga ccactacttt gcctttataa gcggaaaggc
     3661 ttacgggttt gccgtctata tcattcagcg tgaagtcata aatagattgt gcagttgtac
     3721 ttgttgtgtt tttctgtcct tcgtttgctt tacaggcatt taaagaaaag atgaccaaag
     3781 acaacaacaa gaaatatttg ttaaaatttt tcatacttaa atttactaca tttttcattg
     3841 gatttactcc tttatgtatt tttttattta attaacagca agaattgttc caaaaacggg
     3901 tatttgtatt ttgtattttt agtgaagatt agatgtattt aaaatgaatc ctcatttctt
     3961 tagcttcaga atgtgtaact ttataaaaat acatttattg taacatcagt ttttctgata
     4021 taaacctgaa ttatgaaaat tgcagtattg acaaatgaca agcagtcttt agaaacatta
     4081 aacaaaatta tttctaaaga acattataaa ctttatgaac ttaaacagca taaagatgcc
     4141 ataaaattac taaaagcaga taaacctgca tgtatcatca ccgatgtgga attgggtgat
     4201 ttagacggaa ttgaattatg cagcgagctg aaaagcaatg atgaattaaa aggtattcct
     4261 gttgtttttt atgcagacaa ggctgatgac tatacccaaa ttgctgcttt tcagtctgga
     4321 gctgaagaat ttatcgccaa aagccataaa tcgaaactga ttgaagcccg gttaaaacta
     4381 attttcaaac gattttttga caaagacagt attgaaaaaa acatccgcac ttttggcaat
     4441 atcgtgattg atgaagaaca aatggtcatt accaaaaaag gtgatcccct gaaagtttca
     4501 aaaaaagagt tccagttaat cgccttattg acttccaaac caggcaaggt ttttaaacgc
     4561 aataatattc tgctcaaagt atggggtgat gatattattg tgggcgacag aaatattgat
     4621 acacacatca aaaaaatcag aaagaaactg ggaaaaaacc atatcgaaac gatacgaggc
     4681 attggttata aatttattcc atagtttatt tttcagtata ttactaaaat aaaaatcctg
     4741 tttttttata gtacaacttt aatcgaatgg tcacaatttt agcgatgagc tgagtcttaa
     4801 tttctgttac agctaatgaa aaaagcctgc tacttggaat aattcccgta tttttgcagg
     4861 gcagtgttga agactgtatt ttattatgga aaacaaaatt g
//
LOCUS       DAOQII010000194         4829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10395_length_4829_cov_5.479891, whole genome shotgun sequence.
ACCESSION   DAOQII010000194 DAOQII010000000
VERSION     DAOQII010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10395_length_4829_cov_5.479891"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLF48_09025"
     CDS             complement(<1..199)
                     /locus_tag="PLF48_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49509.1"
                     /translation="MKSTLEYLFNGNELSEREAVAILTDIVNQKYNAVQVAAFLTIYN
                     TRLPSTVELKGFRKAIMNLSEK"
     gene            288..791
                     /locus_tag="PLF48_09030"
     CDS             288..791
                     /locus_tag="PLF48_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNF49510.1"
                     /translation="MLNLQEQKKFQSIMTNALLFKGAMAVQLPFAGLSGMQISVLNED
                     ECKVKVPYKFLNKNPFGSTYWAVLGMAAEMASGALLVMFCRGVEPTVSTFVTGCSAQF
                     IKQAKGITTFVCHDGQKIKSAVEGAIESKKAMTFSTEVNGYDKNGELLCAFTFDWGVK
                     QRSKNKE"
     gene            complement(867..1163)
                     /locus_tag="PLF48_09035"
     CDS             complement(867..1163)
                     /locus_tag="PLF48_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNF49511.1"
                     /translation="MIVRIVKLTFREDSVATFEAYFPTVSTTIRNQQGCNLLQAWHDI
                     NNPQIFFTYSLWDSEADLNAYRASEFFGIFWKTVKPWFGEKAEAWSFEKIIDLK"
     gene            complement(1160..1930)
                     /locus_tag="PLF48_09040"
     CDS             complement(1160..1930)
                     /locus_tag="PLF48_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNF49512.1"
                     /translation="MALITLLTDFGERDYDVGIFKGYLYSASPSSKVIDISHTIPPYD
                     IVTGGFFLKNIFRQFPQGTIHIARVYEQGLNSQRILAAKFQNHFFIAPDNGILTLVFD
                     DKPDLMVEVDIKQAKLNTIDEYYCRVVKEITFNNNIGSIGVATQKYLERKAMIPVIQA
                     DRIVGTVAYIDNFGNAITNIHKEEVDRIAKGKKFSINYKKNDFLDTIVQNYSDVPLSY
                     SLARFNNLGFLELCINCGHAGDLLGLMKGDSIQIIFEA"
     gene            1972..2943
                     /locus_tag="PLF48_09045"
     CDS             1972..2943
                     /locus_tag="PLF48_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNF49513.1"
                     /translation="MSELIIKLETVDPVEFFGFNNNKLKILKREFSRLNINARGNKLM
                     VAGNAEELEAFNEKINQLVDYMERYGKISEQHMEEILAGRNPYETEFPSFKDNVLVHG
                     NNGLLVKARTANQQKMVDLSEGNDILFAIGPAGTGKTYTAVALAVRALKNKWVKKIIL
                     TRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPPDKLAYFMQNRVIEVAPLAY
                     MRGRTLDNAFMILDEAQNATQLQLKMFLTRIGPSAKCIITGDLSQIDLPKNQHSGLKH
                     TMRILDNIDGIGIIRLSEEDVVRHRLVKAIIRAFDNDRKEKEHLGEE"
     gene            complement(3000..3449)
                     /locus_tag="PLF48_09050"
     CDS             complement(3000..3449)
                     /locus_tag="PLF48_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNF49514.1"
                     /translation="MKKKILYVLLAVFIVIQFFHPKKNISAQVADYSYMPADVQTIVK
                     KSCFDCHSNNTAYPWYNNIQPVAWWLNRHIVEGKEKLNFDDFQNSKKKDDIAESIEKG
                     EMPLSSYTLIHRDAIMNDQQKQIVINWAKQFEHEKGGNQERGEENEH"
     gene            complement(3544..>4829)
                     /gene="aceK"
                     /locus_tag="PLF48_09055"
     CDS             complement(3544..>4829)
                     /gene="aceK"
                     /locus_tag="PLF48_09055"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNF49515.1"
                     /translation="AIKQIMKSANFNVKFENLERDAQYIFDEMAPVIIFQKSNLFLDR
                     IEVLKSVFYRNRGAFIIGRLFYKNWTMPIVIPLLNEENGIFADSVIYIPADVSILFSF
                     TRSSFMVNTAYPAQLIDYLKAMLTHKAVGELYDSIGYYRHGKTILYRDLMNYIHNHED
                     KFILAPGIKGMVMSVFTLKYFNFVFKVIRDKFEPPKNCSREFVIRKYEEVEINDRVGR
                     MAYAHLFENLEFPRKLFTFELAQELQETCKESVEFKGDKVLIKHVYLERRMTPLNLYL
                     QEANPIDACSVILDYGFCVKELAAANIFPGDLLLKNFGVTRHGRVIFYDYDEIAKVTD
                     CRFRRIPQYTDEDDTRGNFEMIAAEQNDIFPEEFKAFMVPDGPIGDIFLAEHEEIFDP
                     KYWRDIQKNIEAGEYIRFYAYDQYKRFAIKYLTAE"
BASE COUNT         1531 a          887 c          893 g         1518 t
ORIGIN      
        1 ctttttctga aagattcatt attgcctttc taaaaccttt aagttcaact gtagacggaa
       61 gccttgtatt gtatatggtt aagaatgctg ccacctgcac agcattatat ttttgattaa
      121 caatgtcagt caagatagct accgcttctc gttctgacag ttcatttcca ttaaataagt
      181 attcgagtgt agatttcatc cacaaatgta cttattcata ttcaatcatc acattttatt
      241 atcatgcagt cagtaatcat tttgtatttt tagtaaaatt tttatttatg cttaatctgc
      301 aagaacaaaa gaaatttcaa tccattatga ctaatgcctt gttgtttaaa ggggcaatgg
      361 ctgtacagct acctttcgca ggattgagtg gcatgcaaat ttccgtactg aatgaagatg
      421 aatgcaaagt gaaggtgcct tataaatttt taaataaaaa tccgttcggg tcaacctatt
      481 gggcggtgtt gggtatggct gctgagatgg caagcggtgc attattggtt atgttttgca
      541 gaggtgtgga acccactgta tctacttttg taacgggttg ttctgcacaa tttatcaagc
      601 aagccaaggg tattactacc tttgtttgcc atgacggaca aaaaataaaa tctgccgtag
      661 aaggtgcaat tgaaagcaaa aaagcaatga cattctcaac agaagtaaat ggctatgata
      721 agaatggcga attattatgc gctttcacat ttgactgggg ggtgaagcaa agaagtaaga
      781 ataaggagta agggcttagg tgtaaggtat aagtagtaag tataaagcta cttcgcttta
      841 tctgtttgca agatgaaaca cttttgttat ttcaggtcaa tgattttttc aaagctccag
      901 gcttccgctt tttcaccaaa ccaaggtttt accgttttcc agaaaatacc aaaaaattcg
      961 gatgcccgat aggcatttaa gtctgcctcg ctatcccata aactgtatgt aaaaaagatt
     1021 tggggattat tgatgtcatg ccaggcttgt aataaattac agccttgttg atttcttatt
     1081 gtagtgctta ctgttggaaa ataggcttca aatgtagcga cactatcctc tctgaatgta
     1141 agtttgacta tacggactat catgcttcaa atattatttg tatggaatcg cctttcatta
     1201 atcccagcaa atctccggca tgaccacaat tgatgcagag ctccagaaat cccaaattat
     1261 taaaacgagc taaactatat gacaaaggta catcgctata gttctgaact atcgtatcta
     1321 aaaaatcatt tttcttgtag ttgatgctga atttttttcc tttagctatc ctatccactt
     1381 cttctttatg gatattggta atggcatttc caaaattatc aatataagca actgtaccta
     1441 ctatacggtc ggcttgaata acaggaatca tggctttgcg ctctaaatat ttttgagtag
     1501 ccacgccaat cgatccaata ttattattga atgtaatttc ttttaccact ctgcaataat
     1561 attcgtctat cgtattgagt ttagcctgtt ttatatctac ctccaccatc aaatcaggtt
     1621 tatcgtcaaa tactaaagta agtatgccgt tatccggagc aataaaaaaa tgattttgaa
     1681 atttggccgc taaaatacgc tggctgttta agccttgctc gtaaactcga gcaatatgaa
     1741 tagtgccttg cggaaactga cgaaaaatat ttttaagaaa aaaaccgccg gttacaatat
     1801 cataaggagg aatggtgtgg ctgatatcaa ttaccttgga tgaggggctg gcagaataca
     1861 aataaccttt aaaaatgcca acatcataat cccgttctcc aaagtctgtt aataacgtaa
     1921 tcagcgccat agttttaaaa atagtatttt ggcagtaaaa ataagaaata attgagtgaa
     1981 ctgataatta aattggaaac tgtagatccg gtagaatttt tcggatttaa caacaacaaa
     2041 ctaaaaattc tcaaaagaga attcagcagg ttgaatatca atgcccgagg aaataaactc
     2101 atggtagcag gcaatgcaga agaactggag gcatttaatg aaaagataaa ccagctggta
     2161 gattatatgg aacgctatgg taaaatatca gagcaacata tggaagaaat tttagccggg
     2221 cgtaatccgt atgaaaccga atttccaagt tttaaagaca atgtactggt gcatggcaac
     2281 aatggattgc tggtaaaagc aagaacagcc aatcaacaaa agatggtgga tctttccgaa
     2341 gggaacgata ttttatttgc cattggccct gccggaaccg gtaaaaccta cacggctgtt
     2401 gcacttgcag tgcgtgctct gaaaaacaaa tgggtgaaga aaatcatcct gactcgtccg
     2461 gcagttgaag ccggtgaaag ccttggtttt ttgcccggag atttgaaaga aaaaattgat
     2521 ccttatctgc gacctcttta tgatgcactc gatgatatga taccacccga caagctggct
     2581 tatttcatgc aaaaccgtgt tattgaggtg gcgcctttgg cttatatgcg gggcaggacg
     2641 cttgacaatg cttttatgat tttggatgaa gctcagaatg ctacacaatt acaattaaaa
     2701 atgtttttaa cgagaattgg tccatctgca aagtgcatta ttacaggtga tttatcccag
     2761 attgatttac ctaaaaatca gcattcggga ttaaaacata ccatgcgtat attggataat
     2821 atagatggca tcggcatcat ccggttgagc gaagaggatg tggttcgcca tcgtttagta
     2881 aaagctatta tacgcgcctt tgataatgac cgaaaggaaa aagaacatct gggtgaagaa
     2941 tgagctaatc cggcaatgag ctgtgtgaga atgagcaaat atgctaaagc agatgagaat
     3001 taatgttcat tttcctctcc tctttcttgg tttcctcctt tttcatgctc aaactgtttt
     3061 gcccaattaa taacaatttg tttttgctga tcattcataa tcgcatcacg gtgaataagc
     3121 gtataacttg acaatggcat ttctcctttt tctattgact cagcaatatc atctttcttt
     3181 ttagagttct ggaaatcatc aaaattcagt ttttctttac cttccacaat atgtctgtta
     3241 agccaccaag caacaggttg tatattattg taccatggat acgctgtatt attactatgg
     3301 caatcaaagc aagatttttt tacaattgtt tgcacatcag caggcatata actataatca
     3361 gctacttgag cagagatatt ttttttgggg tgaaaaaact gaattacaat aaatacagca
     3421 agcaacacat acaagatttt ctttttcata aaaactaatt tagtacttga agttaaagaa
     3481 aaattctgat ttatcttatt agaaataaaa tttgtattca atccaattaa aaactagtca
     3541 ttattattct gcagtaaggt atttaattgc aaaccgtttg tattgatcat aggcatagaa
     3601 acgaatatat tcaccggctt cgatattttt ctgaatatcc cgccagtatt tggggtcaaa
     3661 aatctcttca tgttctgcta aaaaaatatc cccgatgggt ccatccggca ccatgaaggc
     3721 tttgaattct tccggaaaga tgtcgttttg ttcagcagct atcatttcga aatttccacg
     3781 cgtatcgtct tcgtcggtgt actgcggaat gcgtctgaaa cggcaatccg tcacttttgc
     3841 aatttcatca taatcataaa aaatgacacg gccgtgtcgc gtaacaccga aatttttcaa
     3901 caataaatcg ccggggaaaa tattggctgc cgccaactct ttaacacaaa atccataatc
     3961 taaaattaca ctgcacgcat ctatggggtt agcttcttgc aaatagagat ttaaaggcgt
     4021 cattcttcgt tccagataca catgcttgat taagacttta tctcctttaa attctacact
     4081 ttctttacag gtttcttgca gctcttgtgc cagctcaaaa gtgaataatt tgcggggaaa
     4141 ctctaaattc tcaaacaagt gagcatacgc cattctgcct acccggtcgt ttatttctac
     4201 ttcttcatat tttcttatga cgaactcacg cgaacaattt tttggcggtt caaatttatc
     4261 ccggataact ttaaatacaa aattaaaata tttgagcgta aatacagaca ttaccatccc
     4321 tttgatgccg ggtgctaaaa taaatttgtc ttcatgattg tgaatataat tcatcaaatc
     4381 gcggtacaaa attgttttgc catgccggta ataaccaata gaatcgtata attcgcctac
     4441 tgctttatgt gtcaacatgg ctttgagata atcaatcagc tgcgcaggat aggccgtatt
     4501 gaccataaat gatgaacgtg taaaagagaa caaaatagat acatccgccg ggatataaat
     4561 aacggaatcg gcaaaaatgc cgttttcttc attgagcaag ggtataacga ttggcatcgt
     4621 ccagtttttg taaaacaaac gcccgataat gaaagcccct ctgttgcgat aaaagacaga
     4681 ctttagtact tctattctgt ctaaaaataa attggatttc tggaaaataa taaccggcgc
     4741 catctcatca aaaatgtatt gtgcatcgcg ctccagattt tcaaatttga cgttaaaatt
     4801 cgcacttttc ataatttgct ttatagcta
//
LOCUS       DAOQII010000195         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10449_length_4810_cov_5.162776, whole genome shotgun sequence.
ACCESSION   DAOQII010000195 DAOQII010000000
VERSION     DAOQII010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10449_length_4810_cov_5.162776"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLF48_09060"
     CDS             complement(<1..650)
                     /locus_tag="PLF48_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNF49516.1"
                     /translation="MRSTPSPTIISLLGSLAVASSSTALFLIIATVFRLNYAVASIFV
                     FCTIGFFISFIVFRFVLIRYIYNKIRIIYKTIGKPLKFEQEIKEGSGLMVKTVERDVA
                     EWALNKNKRIRELQKLEQYRKEFVGNVSHELKTPIFNAQGYIETVLDSDMDDPEFIRQ
                     FLEKANSNIDRLESIVSDLLEISKFETGRIQLEKSAFDIVALIKKVFFQYQHLAEQ"
     gene            complement(664..1350)
                     /locus_tag="PLF48_09065"
     CDS             complement(664..1350)
                     /locus_tag="PLF48_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF49517.1"
                     /translation="MAEKTFKILVVDDEPDILEFLSYNLEKDGFEVETAENGKVALEK
                     AKKSQPDIILLDVMMPEMDGIEACRSLREMPQFENTIIAFLTARTEDYSQIAGFETGA
                     DDYISKPIKPRVLVSRLKALLRRYEAKEAKSTFLEVGNIQIDRERYLIIFNGKEMAVP
                     RKEFELLYLLASKPGKVFKRDEILNEIWGRDIIVGDRTIDVHIRKLREKLGEELIKTV
                     KGIGYKFEEV"
     gene            1469..2329
                     /gene="rfbA"
                     /locus_tag="PLF48_09070"
     CDS             1469..2329
                     /gene="rfbA"
                     /locus_tag="PLF48_09070"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNF49518.1"
                     /translation="MKGIILAGGSGTRLYPITYAISKQIMPIYDKPMIYYPMSVLMQA
                     GINEILIISTPHDMPNFQRLLGDGKKYGCNFQYAVQEVPNGLAQAFVIGKEFIGSDKC
                     ALILGDNIFYGTGLEDLLTKNYNPDGGVIYAYHVSDPERYGVVEFDNGFNAVSIEEKP
                     EQPKSNFAVPGLYFYDNEVIDIAENLQPSPRGEYEITDVNKEYLRRGKLKVGILDRGT
                     AWLDTGTFDSLMQASQFVQVIEQRQGLKVGCIEEVAYRKGFINKEQLNEIAQPLLKSG
                     YGQYLLDLMR"
     gene            2501..3790
                     /locus_tag="PLF48_09075"
     CDS             2501..3790
                     /locus_tag="PLF48_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNF49519.1"
                     /translation="MLHPFHYVSAKEALAYIESGNRVFVQGGAQTPLYMLRELAMEKD
                     RLKFVELVFISVQGDIVVNKPEYNGHFDINCMFVSTSVRGAVNTGHADYIPVFLSDIP
                     DLFKKGYLPLDVAIVQVSPPDKHGYCSLGVSVDIARSAVNMAKYVIAQVNPRVPRTHG
                     DSLIHTDRFSAMVYHDEELPEVDYAQKIGQDELKIGKYIAEMIDDGSTLQMGIGTIPD
                     AVLKSLYNHKDLGVHTEMCSDGIIDLFERDVINNKKKVIHPNKTVTGFALGTKRLYNY
                     LDDNPAFVFLDIDYVNDPSVIRRNPKVIAINSAIEVDLTGQVCADSIGTYQYSGIGGQ
                     MDFMRGAALSEGGKPIIALTSRTNKGVNRLVPFLKPGAGVVTSRGHVHYVVTEYGVAN
                     LFGKNLRQRAKALIDIAHPDDREMLEKACHERFKVFV"
     gene            complement(3816..>4810)
                     /locus_tag="PLF48_09080"
     CDS             complement(3816..>4810)
                     /locus_tag="PLF48_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HNF49520.1"
                     /translation="GKVTVDINAKPFEKLSEYHFFKGDIKNLMPNDRVLPYDLITALF
                     TDYAHKSRFVWMPEGISAKYSKDEALDLPVGAVLIKNFYYPHDFRDESKGRKIMETRL
                     LVHRKDGWDNITYVWNDEQTDAELNIVGDSKPVEFTNLQGEHVSTSYGYPNKNQCKNC
                     HNLNNAAMPIGPKVRYLNKDFTYADGVKNQLDKWVEVGYLSGYNKTDNANNKIVNAFD
                     VNAASVEDRAKAYLEVNCAHCHRKEGSASNTGLYLLLSDKNPESWGVMKSPVSAGKGS
                     GNCLYDIVPGKPDESIVTYRMESVEPEIRMPQFGRNLVHKEAVALIRQWISEMK"
BASE COUNT         1380 a          950 c          976 g         1504 t
ORIGIN      
        1 aattgttctg ccaagtgctg atactgaaaa aatactttct taattaaagc aacgatatca
       61 aatgctgatt tttcgagctg aattcgtccg gtttcaaact tagagatttc cagtaagtca
      121 ctgacgatgc tttccagacg gtcaatattg ctgttggctt tttccaaaaa ctgccggata
      181 aattcaggat cgtccatatc cgaatccagt acggtttcaa tatatccttg cgcattaaat
      241 ataggcgttt tcaactcatg cgatacatta ccgacgaact ctttgcgata ctgttccagt
      301 ttctgtaatt cgcgaatgcg tttattctta ttgagtgccc attctgccac atctcgttct
      361 acggttttca ccattagtcc gctgccttct tttatttcct gttcaaattt caagggcttg
      421 ccgatagtct tatatataat tcttatttta ttataaatat accgaataag cacaaatcgg
      481 aaaacgataa atgagataaa aaagccgata gtgcaaaaca caaagataga tgcaacagca
      541 tagttcagcc tgaaaaccgt ggctatgatt aaaaataagg cagtggacga tgatgccact
      601 gccagacttc ctaaaagact tataattgtt ggagaaggtg tacttctcat atgctatttc
      661 ttgctaaact tcttcaaatt tatatccgat gccttttacc gtcttgatta gttcttcgcc
      721 taatttttca cgcagtttgc ggatgtgtac atcgatagtt cggtcgccta ctatgatatc
      781 tcttccccat atttcattga gaatttcatc tcgtttgaat actttcccgg gtttggaggc
      841 aagcagatat aaaagttcga attccttacg cggaacagcc atttctttac cgttgaagat
      901 aatgagataa cgctctctgt cgatctgaat attgcccact tccagaaagg tagattttgc
      961 ttccttagct tcatatctgc gcaataatgc tttaagacgg gaaaccagta ctctcggttt
     1021 aatcggcttg ctgatataat catctgctcc tgtttcaaaa cctgcaattt gtgaatagtc
     1081 ttcagtgcgt gcggtcagaa aagcgatgat agtgttttca aattgtggca tttcccgaag
     1141 gctgcggcaa gcttcaatac cgtccatttc cggcatcatc acatccagca aaataatatc
     1201 cggctggctt tttttggctt tttccagagc cacctttccg ttttctgccg tttcaacttc
     1261 aaagccatcc ttttccaggt tataacttaa aaattccaga atatccggct cgtcatctac
     1321 gactaagatt ttaaacgttt tttctgccat ataatgtagt tgtttaatgt attaacacga
     1381 aattaaccta aaaaaatcaa tctgcaaaat ttcagggcaa acatattcaa ataatgttgt
     1441 ttaactgtgt atttttgttt tttattttat gaaaggaata attttagcag gtggttcggg
     1501 tacgcgttta tacccgatta cctatgccat ctccaagcaa atcatgccga tttacgacaa
     1561 acccatgatt tattacccga tgtcagtact gatgcaggca ggtatcaacg aaatattaat
     1621 tatttccaca ccacacgata tgccaaactt tcaacgctta ttgggtgatg gaaagaaata
     1681 cggatgcaat tttcagtatg cagtgcaaga agtaccaaat ggtttagcac aagcattcgt
     1741 gattggcaaa gaatttatcg gcagtgataa gtgcgcattg atattgggag ataatatctt
     1801 ttatggtacg ggtttggaag atttacttac taaaaactat aacccggacg gtggtgtgat
     1861 ttatgcatat catgtctctg acccggaacg atatggtgtt gtagaatttg ataatgggtt
     1921 taatgctgtt tctattgaag aaaaacccga acagccaaaa tcaaattttg ccgtaccggg
     1981 attatacttt tatgacaatg aagtaattga tattgctgag aacctgcaac ctagcccacg
     2041 gggcgaatat gaaattacgg acgtaaacaa ggaatatttg cgcagaggaa agttaaaagt
     2101 aggcatactc gacagaggta cagcctggct ggatacgggt actttcgatt cgctcatgca
     2161 ggcatcacag tttgtgcagg taatagaaca acggcagggg ttaaaagtag gttgtattga
     2221 ggaagtagcc tatcgcaagg gttttattaa caaagaacaa ctcaacgaaa tagcccagcc
     2281 cttgcttaaa agtggctatg ggcaatacct gctggatttg atgaggtagc tgttttgtta
     2341 tagctgcatt ccgtttatgt tcgggttatg atatatgaat tgaatccctg tctttgataa
     2401 gattgattat ttgtgagagc agaatttcac ccaaaattca ttttatattg atatgcatag
     2461 ccggattttg atgtaaactt gcaacctcaa ttttattgct atgctacatc cttttcacta
     2521 tgtatctgcc aaagaagcac ttgcttatat cgagagtggt aatcgtgtat ttgttcaggg
     2581 tggtgcacaa acgcccttat acatgttgcg agaacttgca atggagaaag accggctcaa
     2641 attcgtagag ttggttttta tttcggttca aggtgatatt gtagtgaata agcctgaata
     2701 taacggtcat tttgatatca actgcatgtt tgtgagtacg agtgtgcggg gtgctgtcaa
     2761 tacaggtcat gccgattata ttcctgtttt tctgagtgat attcccgatt tgttcaaaaa
     2821 agggtactta cctctggatg ttgccattgt gcaggtatca ccgcccgaca aacatggcta
     2881 ctgctcgtta ggagtaagtg tagatatagc acgcagtgcc gtaaatatgg caaaatatgt
     2941 aattgcgcag gtcaatcctc gtgtacctcg tacgcatggc gacagtctta tacataccga
     3001 caggttttca gccatggtat atcatgatga agaattaccg gaagtagact atgctcaaaa
     3061 aattggtcaa gatgaactta aaatcgggaa gtatattgcc gagatgatag atgatggcag
     3121 caccttgcaa atgggcatag gcactatacc cgatgccgtg ttgaagtcgc tttataatca
     3181 taaagattta ggtgtgcata ctgaaatgtg cagcgatggt attatcgatt tatttgagcg
     3241 ggatgtcata aacaataaaa agaaagtaat ccatcccaat aaaacagtta ccggctttgc
     3301 ccttggtacc aaacggctat acaattattt agatgataat cctgcttttg tttttctaga
     3361 tattgactat gtaaatgacc catccgttat caggcgcaac ccaaaggtaa tagctatcaa
     3421 cagtgcgata gaagtggact taaccgggca agtatgtgcc gacagtatcg gcacgtatca
     3481 atatagtggc atcggcgggc aaatggactt tatgcgaggt gctgctttga gcgaaggagg
     3541 aaagccgata attgcattga cttccagaac caataaagga gtaaaccgtt tagtgccttt
     3601 tcttaaaccg ggagcaggcg tggttacttc aagaggacat gtacattatg tggtaacaga
     3661 atatggcgtt gccaatttgt ttggcaaaaa tctcagacag cgggcgaagg cattgataga
     3721 tattgcacat cctgatgaca gagagatgct ggaaaaagcc tgccacgaaa ggtttaaggt
     3781 atttgtgtaa tattttaagt ttgtgcaatc agatattact tcatctcgct aatccattgg
     3841 cgtatcagtg ccacggcttc tttatgcact aaatttctac caaactgagg cattctgatt
     3901 tctggttcta cactttccat tcgataggtt acaatagatt catccggctt gccgggtact
     3961 atatcataca gacagtttcc gctacctttg cctgcagaaa ccggactttt catcaccccc
     4021 cagctttcag gatttttatc actcagcaat aaatataaac cggtattact ggcactgcct
     4081 tctttgcggt ggcaatgggc gcagttgact tccagatatg ccttagcccg gtcttctaca
     4141 gaggctgcat ttacatcaaa tgcatttaca atcttgttat tcgcattatc agtcttgttg
     4201 tagccggata aatagccaac ttccacccat ttatccagct ggttttttac tccatcggca
     4261 taagtaaaat ctttatttaa gtatcgcact tttggtccaa tcggcatggc ggcattattg
     4321 aggttgtggc agtttttaca ttgattttta ttggggtagc cataactagt actcacatgt
     4381 tcgccttgca aatttgtaaa ctcaacaggc ttggaatcgc caacaatatt gagctcggca
     4441 tcagtttgtt catcattcca aacataagta atattgtccc aaccgtcttt tctgtgtacc
     4501 aaaagccggg tttccataat ctttctgccc ttagattcat ctctgaaatc gtgggggtaa
     4561 taaaaattct taattaaaac ggcacccacc ggcaaatcca gtgcttcgtc tttactatac
     4621 tttgcagata tgccttccgg catccacaca aaacgagatt tgtgggcgta atcggtaaaa
     4681 agtgcagtaa ttaaatcata aggaagaact ctgtcattgg gcatcaggtt ttttatatca
     4741 cctttaaaaa aatgatactc cgataatttt tcaaaaggct tggcatttat atctaccgtt
     4801 acttttccgg
//
LOCUS       DAOQII010000196         4809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10451_length_4809_cov_5.214346, whole genome shotgun sequence.
ACCESSION   DAOQII010000196 DAOQII010000000
VERSION     DAOQII010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10451_length_4809_cov_5.214346"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PLF48_09085"
     CDS             <1..1474
                     /locus_tag="PLF48_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF49521.1"
                     /translation="GNSGVSVCSLDDAKKLYSGFDLCSPNTSVSMTINGPAPTICAFF
                     MNAAIDQQCEKYIRENNLVDEVNSKIDKIYQDKKAIRPSYNGTLPEGNDGLGLLLLGV
                     TGDQVLPEEVYEKIKTFALSQVRGTVQADILKEDQAQNTCIFSTEFSLKMMGDVQEYF
                     VHHKVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTFIEYYLSRGMHIDDFAPNLS
                     FFFSNGIDAEYAVIGRVARKIWAKALKYLYKANDRSQKLKYHIQTSGRSLHAQEIDFN
                     DIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENP
                     LQGSFIIEELTDLVEEAVYAEFDRITERGGVLGAMETMYQRSKIQEESMYYEMKKHTG
                     ELPLIGVNTFLSSKGSPTILPQEVIRSTEEEKQLQISNLNALKVRNKSEASVYLQQLQ
                     LAALENKNMFDVLMETVKYCSLGQITNALFDVGGQYRRNM"
     gene            2476..2682
                     /locus_tag="PLF48_09090"
     CDS             2476..2682
                     /locus_tag="PLF48_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49522.1"
                     /translation="MNDFLHPAFLIRLAIAIAYIALAAVLFFLPVTKLPILSDKTKLA
                     LSVLLFAYGLFRLYRAFKFKKEME"
     gene            2679..3599
                     /locus_tag="PLF48_09095"
     CDS             2679..3599
                     /locus_tag="PLF48_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF49523.1"
                     /translation="MKIWLQLLYSLIFFVLVSCNSSQKNEKQKNLNDWESGTARIHAD
                     TNLRYLLDQIIPVYEHFHPKSKLIVEYNNEDALISDFKADKNKIILVSTAFDPEELAY
                     CEQIQKAKIIQYTFGYDAIAVIASRQNPDSVFYLNQLAEMLQNKKTPLVFDHVQSGIA
                     KALMQQVKINPALFKNALVVNNLKDVVNYVVAKPESIGFIPFNLVSNDEDVVSKRLLE
                     KIKLLPVHLNDTTFYLSQNDLANGQYPLYRPISIIIGNTDEVNALGFADFLMNQQVSK
                     IMIHSGVIPTLMPERLYQYTEEFHPEGKKK"
     gene            complement(3602..4540)
                     /locus_tag="PLF48_09100"
     CDS             complement(3602..4540)
                     /locus_tag="PLF48_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNF49524.1"
                     /translation="MNFSLTQLEYVLAIDNYRHFAKAADACLVTQPTLSMQLKKLEEE
                     LGFELFDRTKQPIIPTDAGKIIIEQARTILRETKKLEHIAQTFDNSFDGNFSVGIIPT
                     IAPYLLPYFLGDFIKKYPNIRLHLQELKTEEIIFKLNKDELDAGILATPLHESNLIEE
                     PVFYEEIKLYAHYKHLLSNKKNLKPDDIMRPDIWMLTEGNCFRNQIINICTQPQEITK
                     HLHYDSGSLETLKKMVDMEGGFTLLPELAVLELSNKKQEQISEFRKPKPLREISLVYA
                     RSVAKMKYIHVLKKLIQESIPDYLQNKKRGTVVEWK"
     gene            4624..>4809
                     /locus_tag="PLF48_09105"
     CDS             4624..>4809
                     /locus_tag="PLF48_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HNF49525.1"
                     /translation="MALTKIGLDQQQSEKLADGLNDLLASFQIYYQNLRGLHWNIKGK
                     HFFELHVKFEELYTDAQL"
BASE COUNT         1535 a          874 c          888 g         1512 t
ORIGIN      
        1 cggcaatagc ggcgtgtctg tatgcagttt ggatgatgca aaaaaactgt attccggttt
       61 tgatttgtgt tcgcccaata cctctgtatc catgacgatt aacggccctg cacccacaat
      121 ttgtgctttt tttatgaatg ccgccattga ccagcaatgc gaaaaatata tccgtgaaaa
      181 taatttagta gacgaggtaa acagcaagat agataaaatc tatcaagata aaaaagcgat
      241 aagaccaagc tataatggta cattgcccga aggaaatgac ggcttgggtt tacttctgct
      301 gggcgttacg ggcgaccagg tattgcctga agaggtctat gaaaaaatta aaacgtttgc
      361 attatctcaa gtgcgtggta cggtgcaagc ggatatactg aaagaagatc aggcgcaaaa
      421 tacctgtatt ttttctactg aattttcatt gaaaatgatg ggcgatgtac aagagtattt
      481 tgtacatcat aaagtccgca atttttattc ggtttctatt tccgggtatc acatcgcaga
      541 agccggagcc aacccgattt cacagctggc gtttaccttg gcaaatggct tcacgtttat
      601 tgagtattac ttatcgcgag gcatgcatat tgatgatttc gctccaaatt tatctttctt
      661 cttttctaac ggcatcgatg ctgaatatgc ggtgattggt cgtgttgccc ggaaaatatg
      721 ggcaaaagca ttgaagtatt tatacaaagc caacgacaga agccagaaat taaaatacca
      781 tatccaaact tcgggtagaa gcttacatgc acaagaaata gattttaacg acatccgcac
      841 aactttgcaa gccctgtacg ccatttatga caattgcaac agtttgcaca ccaatgcgta
      901 tgacgaagcc atcaccacac caacggaaga aagtgtacgc cgggcaatgg cgatacagct
      961 gatcatcaat aaagaattag gcttggcaaa aaatgaaaat ccactgcaag gttcatttat
     1021 tatagaagaa ctgaccgatt tggtagaaga ggccgtatat gcagagtttg accgtattac
     1081 ggagcgcggt ggtgtgttgg gcgctatgga aaccatgtat cagcgcagca aaatacagga
     1141 agaaagtatg tattatgaaa tgaaaaaaca taccggagag ttgccgctga ttggtgtcaa
     1201 tactttttta tcttccaagg gttctcctac tattttgccg caagaagtaa tacggagcac
     1261 ggaagaagaa aagcaattac aaatcagcaa cctgaatgcg cttaaagtac gcaataaatc
     1321 tgaagcctct gtttatttac aacaattaca actcgcagcc ttagaaaata aaaacatgtt
     1381 tgacgtgctg atggaaaccg taaaatattg cagtttagga caaatcacca atgccttgtt
     1441 tgacgttggc ggacaataca gaaggaatat gtagaattaa tttggcaatt tgaagatgtg
     1501 caaatttgct cattagcttg ttccatttta tttgccgtaa atagtactga cttcttttta
     1561 tttctgaaaa gatactcgtt attttgattt tagcgaatgt gaaaagaatt tacaaattgg
     1621 caaattcaca caaaaatttg gaagtacaag tacgccacac tttttgcatt actgcatgtt
     1681 tgttttgatt cttcggaata gtgttttttt tgacatactt gcattagttg agtcagagct
     1741 aatgtaagcg actaaataga aagaatagaa aaaaccatgg agttaaagct cctaataaat
     1801 ttaaaagaaa aaatttaaca aaagtttcag ttggtgtctt ttgtaaaaaa aacaatacaa
     1861 cactatacaa aaacaagata acaaatattg aagagaaaag aactattgtt aaaggtaaaa
     1921 aaacaagaag tttgtctttt ccaaaaacaa cacacaaata atgaaacaat agtgccacaa
     1981 tttcaattat tattaaaagt atgatatata aagttttatt catttttaat attttctttt
     2041 ccttttgttg gtttgcctca aataaaccat aatccgaatt gtttcgcaat tttatttcac
     2101 ttcttttata aaattacaat atttaaatta aaaaatttct gtactataga gtgtagcagg
     2161 ttttttaaga acacgaaatg cacagttgat acggattttt tatccgagtt tatcacctta
     2221 tccggttaat ctgtgttcta ttctttctgt gtgaaaactt aatttgtgtt taaaattgag
     2281 atctttagcc taaatgctta tttattgaac aaagtgtact agtctaccgt atcgcatttt
     2341 tctttatcct aaaaaaagca atggatgaag cgactgtata atacctcatt aactctttag
     2401 cagattatcc ggttaattaa tttaaatcca agttttgtcg tctgtggact gattacaatt
     2461 ccttattttt acagcatgaa tgattttttg catcctgctt ttctcatccg tttggcaata
     2521 gccattgcct atattgcatt ggcagctgta ttgttttttc tgcctgtcac aaaactaccc
     2581 atactttcag ataaaactaa attagcgctt tcggtgttac tgttcgcata cggattgttc
     2641 aggctttacc gtgcttttaa gtttaaaaaa gaaatggaat gaaaatctgg cttcaacttc
     2701 tgtactcgct aatatttttt gttttagtat cctgtaattc ttctcaaaaa aatgagaaac
     2761 agaaaaacct caacgactgg gaatcaggca cggcacgcat acatgccgat acaaatttgc
     2821 gctatctttt agaccagatt attcctgttt atgagcattt ccaccccaaa tcaaaactca
     2881 ttgttgaata taataacgaa gatgctttaa tcagtgattt taaagctgac aaaaataaaa
     2941 taattctagt cagcactgcc tttgacccgg aagaactggc ttattgcgaa caaatacaga
     3001 aagctaagat aatacaatat actttcggct acgatgccat tgcggttatc gcatcccgcc
     3061 aaaatcccga ttcggttttt tatctcaatc agttagcaga aatgctgcaa aataaaaaaa
     3121 caccgctcgt ttttgatcat gtacaatccg gtattgccaa ggcattgatg cagcaagtga
     3181 aaataaatcc tgctttgttt aaaaatgcat tagtggtgaa caaccttaaa gatgttgtaa
     3241 actatgtggt ggccaaaccc gaaagcatcg gttttattcc gtttaatctg gtaagtaatg
     3301 atgaggatgt agtatccaaa cggctcttgg aaaaaattaa attactgcct gttcacttaa
     3361 atgatactac tttctatttg tcgcaaaacg atttggcaaa tggacaatac ccactctacc
     3421 gaccaatctc tattatcatc ggcaacaccg atgaagtaaa tgcccttggt tttgccgatt
     3481 ttcttatgaa tcagcaagtt tctaaaataa tgatacacag cggtgtaata cctaccctta
     3541 tgcccgaaag attatatcag tacacagaag aatttcatcc tgaaggaaag aaaaaataag
     3601 cttatttcca ctctaccacg gtgcctcttt tcttgttttg taaatagtcg ggaatgcttt
     3661 cttgtatcag ttttttcaaa acatgaatat atttcatctt ggccacgctg cgggcatata
     3721 ccaggctgat ttcgcgcagt ggttttggct tgcggaattc agaaatctgt tcttgttttt
     3781 tattggataa ttctaaaacg gcaagttcgg gcaacaaagt aaaaccgcct tccatgtcca
     3841 ccattttctt cagcgtttca agactgccac tgtcgtagtg taaatgtttg gtaatttctt
     3901 gcggttgcgt gcagatattg ataatctggt tgcgaaaaca atttccttct gtcagcatcc
     3961 agatatccgg gcgcattata tcatcaggct tcaggttttt tttattgctc aatagatgct
     4021 tataatgcgc atagagtttt atttcttcat aaaaaacggg ttcttctatc aaattgcttt
     4081 cgtgtaaggg cgtagctaaa attccggcat ccagctcgtc tttattcagc ttaaaaataa
     4141 tttcctctgt tttcagttct tgcaaatgca aacggatgtt cgggtatttt ttgataaaat
     4201 cacctaaaaa atagggcaac aaatatgggg caatggtggg tataatacca acgctaaaat
     4261 tgccgtcaaa gctattatca aatgtctggg ctatatgctc cagcttcttg gtttcgcgca
     4321 aaatggtgcg ggcttgttct attataattt tgccggcatc tgttggaatg atgggttgtt
     4381 tggtccggtc aaaaagttca aaccccagtt cttcttccaa ttttttgagc tgcatgctta
     4441 acgtgggttg ggttaccaga caagcatcag cggctttggc aaaatgacga taattgtcaa
     4501 tcgccaaaac atattctaat tgtgtaagcg aaaaattcat aaacaaattc tattgaatca
     4561 taaaattaat caattttatt tattaaaaca agtggtgtac ttttgtaatc taaaataacg
     4621 aacatggcac ttacaaaaat aggcttagac caacaacaat ccgagaaatt agccgacggg
     4681 ctgaatgatt tattagcctc ttttcaaatt tattatcaaa atttgcgagg tttgcactgg
     4741 aatataaagg ggaaacactt tttcgaattg catgtcaaat ttgaagagct atacaccgac
     4801 gcacagctg
//
LOCUS       DAOQII010000197         4798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10484_length_4798_cov_4.137887, whole genome shotgun sequence.
ACCESSION   DAOQII010000197 DAOQII010000000
VERSION     DAOQII010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10484_length_4798_cov_4.137887"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1087
                     /locus_tag="PLF48_09110"
     CDS             209..1087
                     /locus_tag="PLF48_09110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNF49526.1"
                     /translation="MATKTKTLEQSATKAYNAEEIKVRRVNFQFNNKTPRFYYKNNPF
                     STHFINVLHIVFPTGERLFVNSVLKNAKQIEDEKLKKQVRNFCGQEGIHSSMHERFWK
                     ILKDNNYDISGYEKHIDDVLHKVVAKVKLPMKKNDFDLVATVCLEHFTALFGHAIFNH
                     VETNKEVVPEDIAELFQWHAAEEIEHKHVAFDVLKTVDDQEYIKRVIAMPLVTALLYG
                     YIAGGMGMLFYQDRKKLEYRKLPKQFFEFATGLFSQLHGDMSTEFLKFFKKDFHPSDL
                     NDYYLAEEFFKDKSYA"
     gene            complement(1252..1842)
                     /locus_tag="PLF48_09115"
     CDS             complement(1252..1842)
                     /locus_tag="PLF48_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49527.1"
                     /translation="MNLNALEKLKTVFAYCHKQAQFYTSWGHVDILQIAIKENDGTIF
                     FTKENSNLSSLNNDEVIQDKENASLFNQIFQSRKNINCIFITRQEYASQIKAEIPPIL
                     DDQAQLLGVSIRVAKNEKEILHALKNRFAAILPDGNSICIGVNYEDAYVAAQLLEKTS
                     KAFIEAQYAGGAKSINKIEAWLMQKFYQLKYAKQAK"
     gene            complement(1842..2423)
                     /locus_tag="PLF48_09120"
     CDS             complement(1842..2423)
                     /locus_tag="PLF48_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNF49528.1"
                     /translation="MNITEAKTAVCHAGVQLLHEGLVARTWGNVSIKVNETQMVITPS
                     GRKYEELTPDDMVLVDFFTLKYEGKHKPSSELKLHAEVYKTRPHINAVIHTHQMNASI
                     LAAAQCDVLVLNEEHQQILGAKVIKAAPYALPNTKKITVETAKAIEKSNAALMSNHGV
                     VCIGINLTDAFLVSKILEAASINYLASLKNKSA"
     gene            complement(2420..3946)
                     /locus_tag="PLF48_09125"
     CDS             complement(2420..3946)
                     /locus_tag="PLF48_09125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNF49529.1"
                     /translation="MIKNFLAYDVGTSSVKAVLLNSRGVILATASADYPLYTPQPGWV
                     EQVPTDYWNAICKTTNELIQTSGIDKHAIKAMAFSTQAMGIIPVDKNGNVLYNNISWV
                     DGRAEEYALIAMKRCGGKGVFKAIAGVELTGKDVIPKLLWMKEKQAAVFQNTYKFLDV
                     NGYLKYKCTGKLVAEWSGACSYAFDVKKKDWERLLFRLTKIGLDKLPDLVRSTDLVGC
                     LTEQAATELGLTTSVEVYGGCDDTQAAAVGTTAIGEGEAHIYVGTSAWVGVSTKNALK
                     FKNGAFCLQSADPAKNIVVCITESAGINTQWLLDNFYKQEKVNTAVSVFDILENDMRS
                     VPAGSDYLIVTPWFLGERCPVSTTTTRSTIFNLTHQHTRAHITRAHFEGIAYNLRWSM
                     QNLERDFGFKIETLKITGGGSVNKVWMQLLADITQRKISTTSQPLNAGAFGAAMCAMV
                     GSKTCISFAAIQELIQIEHTFEPNKENYAIYDELFVAYQDIFHQLNKTYQTINKPRFQ
                     "
     gene            complement(3980..>4798)
                     /locus_tag="PLF48_09130"
     CDS             complement(3980..>4798)
                     /locus_tag="PLF48_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF49530.1"
                     /translation="MMHWHTQAVPPNDIDALERTLKWNRLRGGTAAVILEPVGPESGT
                     YPVYKEYAQQARELCNKYGALLIFDEVVTAFRIGMSGAQGYFGVEPDLTIFGKVVAGG
                     YPSAGGIGGKAIYMDGLAAGLQGGKKVKVGGTMAANPLSCCAGYHTLVEIEKNNACEK
                     AGRAGDRITQGLNMLIDKYGLPFVAYNQGSICHLEAAGTLFVKIKLMKIKRVLAEINE
                     RKKLMEEYGAAYMAEGIVTLAGSRLYCSMADTDEVVDDALNRFEKVFKNVKLAN"
BASE COUNT         1443 a          973 c          802 g         1580 t
ORIGIN      
        1 caaattgata attatgaact ggcagaagaa ttactgaaag gcaaatccta tgcttaaagt
       61 aatgttgaat gatgaatgat gaatgatgaa tgttgaatga taaatgataa atgatgaatg
      121 ataaatgatg aattttagat tttaaattac ttcgttcgat taaccaatca ccaattacca
      181 atctctaatc accaataacc tacataaaat ggctactaaa actaagacac ttgaacaatc
      241 tgctactaag gcttacaatg ctgaagaaat aaaagtacga cgggtaaact ttcagtttaa
      301 caataaaacg cctcgttttt attataaaaa caatccattc agcacacact tcatcaatgt
      361 gttgcatatc gtatttccta cgggcgagag attatttgta aattctgtac tcaagaatgc
      421 caaacaaatt gaagatgaaa aattaaaaaa gcaagtgcgt aacttttgcg gacaagaagg
      481 tatacattcc agtatgcacg agcgattttg gaaaatctta aaagataata attacgatat
      541 ttcagggtat gaaaagcaca ttgatgatgt tttgcataag gtcgttgcca aagtaaaatt
      601 acccatgaaa aagaatgatt ttgatttggt tgctacggta tgtttagaac attttacggc
      661 attattcgga catgcaattt tcaatcatgt ggaaacgaat aaagaagttg tgccggaaga
      721 tattgctgaa ctttttcaat ggcatgctgc cgaggaaatt gaacataagc atgttgcttt
      781 tgatgtattg aaaaccgtag atgaccaaga atacatcaag cgtgtaattg ctatgccatt
      841 ggttacggcg ttgctgtatg gatacattgc aggcggtatg ggcatgctgt tttatcaaga
      901 cagaaaaaaa ttagaatacc ggaaattacc taaacaattt tttgaatttg ccacaggatt
      961 attcagtcag ttgcatggtg atatgtctac agagttttta aaattcttca aaaaagattt
     1021 tcatccttca gacttaaatg actattattt agcagaagaa ttttttaaag acaagtctta
     1081 tgcttagtca tgttgaatga ttaatgatta atgttgaatg ttgagtgatg aatatttaat
     1141 acgctataaa atcttgaaag attaagacaa taaacaaatt aatatacttc acattttatt
     1201 aactacgtct taagtactta ctacttgaga cttacacctt actactatca ctcacttcgc
     1261 ttgcttggca tatttcagct ggtaaaactt ttgcatcagc caggcttcaa ttttgttgat
     1321 agattttgct ccgcctgcat attgagcttc tataaatgct tttgaggttt tttctaataa
     1381 ctgtgcagcg acatatgcat cttcgtaatt tactcctata caaattgaat tgccatcagg
     1441 cagtatggcc gcaaacctat tttttaaagc atgtaaaatt tccttttcat tttttgctac
     1501 tcgaatactg acacccaaaa gttgagcttg atcatctaag ataggtggaa tttcagcttt
     1561 aatttgtgat gcatattctt gtcgggtgat aaaaatgcaa ttaatatttt ttcttgattg
     1621 aaaaatctga ttaaacaatg acgcgttttc cttatcctgt atgacttcat cgttgtttaa
     1681 cgatgaaaga ttggagtttt cttttgtaaa aaaaatagtt ccgtcatttt ccttaattgc
     1741 aatttgcagt atatctacat gaccccaaga tgtataaaat tgagcttgtt tatggcaata
     1801 agcaaataca gttttcagtt tttcaagtgc attaagattc attatgccga tttatttttt
     1861 aaggatgcta aataatttat actcgccgct tccaagattt ttgaaaccaa aaatgcatct
     1921 gttaaattta tacctataca aactacacca tgattagaca tcagtgcggc attagatttt
     1981 tcaatggctt tcgctgtttc tacagttatc ttcttagtat tgggcaatgc ataaggagca
     2041 gctttaatca ccttagctcc taatatttgc tgatgttctt catttaagac gagcacatca
     2101 cattgagctg ctgccaagat agatgcattc atttgatggg tatgaataac ggcattgata
     2161 tgtgggcgag ttttatacac ctcggcatgt aatttcaact ctgatgatgg tttatgcttg
     2221 ccttcatatt tcaacgtaaa aaaatcaacc aataccatat catccggtgt tagctcttca
     2281 tattttctgc cactgggcgt aatcaccatt tgcgtttcat ttacttttat gctcacattt
     2341 ccccaagtcc gagctactaa tccttcgtgt agtaattgta cacctgcatg gcatacagca
     2401 gttttggctt cagttatatt cattggaatc tgggtttatt tatagtttga taggttttat
     2461 tcagctgatg gaatatatcc tgataagcaa caaacagctc atcataaatt gcataattct
     2521 ccttgttcgg ctcaaatgta tgctcaattt gaattagttc ttgaattgct gcaaatgaga
     2581 tacatgtttt gcttcccacc attgcacaca tggctgctcc aaatgctccg gcattaagcg
     2641 gttgagaagt tgtgcttatt tttctttggg taatatctgc caataattgc atccaaacct
     2701 tattgacact gccgcctcct gttattttca gggtttcaat tttaaaaccg aaatctcgct
     2761 ctaaattttg catagaccaa cgtaaattat aagctatgcc ttcaaaatga gctcttgtaa
     2821 tatgtgcacg ggtatgttga tgcgtcaaat taaaaatcgt agagcgtgtt gtcgtggtac
     2881 tcaccggaca tctttcgccc aaaaaccaag gtgttactat caaataatct gaacccgccg
     2941 gaacagaacg catgtcattt tctaaaatat caaaaacaga aactgctgta tttacttttt
     3001 cttgcttata aaaattatcc agcaaccatt gcgtattgat acctgcagat tcagtaatac
     3061 aaaccactat atttttagca ggatcggcac tttgcaaaca gaatgctcca tttttaaatt
     3121 tcaatgcatt ttttgtactg acacccaccc atgcagaagt ccctacataa atatgagctt
     3181 caccttcacc gatggctgta gtacctaccg cagctgcttg tgtatcgtcg cagccgccat
     3241 aaacttcaac tgaagtagtt agtcccaatt cagtagctgc ttgttctgta aggcaaccta
     3301 cgaggtcggt agagcgaact aaatccggta atttatctaa tcctattttt gtcaagcgaa
     3361 acagcagtcg ctcccagtct ttctttttta catcaaaggc atacgaacaa gcacccgacc
     3421 actccgccac caatttgccc gtgcatttat atttcagata gccgtttaca tccaaaaact
     3481 tgtacgtatt ttggaaaact gcagcttgtt tttctttcat ccaaagtaat ttcggaatca
     3541 catcttttcc tgtaagctcc acgcctgcaa tggctttaaa tacccctttc ccgccgcagc
     3601 gtttcatggc aattaatgca tattcttctg ctcttccatc tacccaagaa atattgttat
     3661 acaatacatt tccatttttg tctaccggaa taatgcccat cgcctgcgta gaaaatgcca
     3721 tggcttttat agcatgttta tctataccgg atgtttggat aagttcattt gtcgttttac
     3781 aaatggcatt ccagtaatcc gttggcactt gctccaccca acccggttgg ggtgtatata
     3841 aaggataatc tgccgaagca gtagccaata ttaccccacg gctattaagc aaaacagctt
     3901 taacactact cgtaccgaca tcgtaagcta ggaagttttt aatcattgta tgttattagt
     3961 agctgattag ctgatttgat taattagcta atttaacatt tttaaatacc ttttcaaatc
     4021 tgttcaatgc atcatctacc acctcatccg tatctgccat actgcaatac aaacgacttc
     4081 ccgccagtgt aacaatgcct tcagccatat atgccgctcc gtactcttcc atcagttttt
     4141 tgcgttcatt aatttcagct aaaacacgct ttattttcat caattttatt ttcacaaata
     4201 aagtacctgc tgcttccaag tggcatatag atccttgatt gtaagcgaca aatggaagtc
     4261 catatttatc aatcagcata ttaagacctt gagtgattct gtcgcctgcc ctgcccgcct
     4321 tttcacaggc attgtttttt tctatttcta ctaaggtatg atagccggcg caacaactca
     4381 atggattggc agccattgta ccgcccactt ttactttttt tccgccttgc agtccggcag
     4441 ccaagccgtc catataaata gctttaccac ctatcccacc tgccgacgga taaccaccgg
     4501 ctacgacttt tccaaaaatc gttaaatcag gttcaacacc aaaataacct tgtgcgccac
     4561 tcattccaat tctgaaagca gtaacgactt catcaaatat caagagcgca ccatatttgt
     4621 tacacaactc cctagcttgc tgtgcatatt ctttatatac gggataagtg ccactttcgg
     4681 gaccaacagg ctctaagatt acagcagcag tgccacctcg taatctattc cattttaatg
     4741 ttctttccaa agcatcaata tcgtttggtg gaaccgcttg cgtatgccaa tgcatcat
//
LOCUS       DAOQII010000198         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10494_length_4795_cov_5.516245, whole genome shotgun sequence.
ACCESSION   DAOQII010000198 DAOQII010000000
VERSION     DAOQII010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10494_length_4795_cov_5.516245"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLF48_09135"
     CDS             complement(<1..778)
                     /locus_tag="PLF48_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNF49531.1"
                     /translation="MLNYQQHPHFTVHNLPYGIYSTAIKIKRAGVAIGDFIIDLSILY
                     KSGKLPFIQTDVFLQDSLNDFIALGKSIHHQTRSTIQHLLENEPDFISTYDGLIDKQS
                     DAKMYVPVKVGDYTDFYASEEHASNVGKMFRPTMPPLMPNWKHMPIAYHGRSSSIITS
                     NTPIKRPSGQILLGETPVYAACKTLDIEIELGYIIGKENKLGEPVDIQNATEYIFGAV
                     MLNDFSARDIQRWEYQPLGPFLGKNFATAISPWIITYEALQ"
     gene            complement(791..1138)
                     /locus_tag="PLF48_09140"
     CDS             complement(791..1138)
                     /locus_tag="PLF48_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF49532.1"
                     /translation="MAELVKEFNDYRSKMNDVILEQKNLTLNRFFNLDMHAYADGALD
                     AKTKEMLGLVASMVLRCDDCIKYHLGKCHDLQISTEQIYEIFAIANLVGGSICIPHTR
                     RAAEYWQALISEN"
     gene            complement(1209..1547)
                     /locus_tag="PLF48_09145"
     CDS             complement(1209..1547)
                     /locus_tag="PLF48_09145"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF49533.1"
                     /translation="MENFELNTEKIERAAELLKAVAHPLRLKIIKMINDKKEVNVNVI
                     YNTLRIEQSITSQHLKVLRGVDVVKTRRDGKKIFYSLNYDRLDAMNRGIDLFEDWNTV
                     AKKAPAKKKK"
     gene            complement(1896..2243)
                     /locus_tag="PLF48_09150"
     CDS             complement(1896..2243)
                     /locus_tag="PLF48_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF49534.1"
                     /translation="MKINKTKEMSANINSIMQPELKIDYQVLRKSVLLLRAINHKMRQ
                     EMIRIIDETGKITVTDLYVRMRLEQSVASQHLALLRRSGVVFTTRDGKYIYYSVDQSR
                     MEEINRLLGELVR"
     gene            complement(2343..3026)
                     /gene="tsaB"
                     /locus_tag="PLF48_09155"
     CDS             complement(2343..3026)
                     /gene="tsaB"
                     /locus_tag="PLF48_09155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNF49535.1"
                     /translation="MPDVLLLHIEAAYGNTSFAISKNGICTDVLFSAQSNQTADVAHS
                     MVQQILDNNKLKFSDIHAVSVSSGPGSYTGLRIVAAMSKGICFAQNLPLIAIPTLKAM
                     AYGCIHRYGQTGYDWYIPMIDARRMEVFTTYYNQDLKAEKSFSSLILDEEWLAGLGCE
                     KKIIFFGNGINKLQLPDEFKDILLFHDFIISAEDQSLLAFQRYTARQFEDIAYFEPNY
                     FKAFYTPSN"
     gene            complement(3125..>4795)
                     /locus_tag="PLF48_09160"
     CDS             complement(3125..>4795)
                     /locus_tag="PLF48_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF49536.1"
                     /translation="IELLYKFGMGSAVYQGANRYNVKNITFHQPKLQVDYKGLSLKYY
                     STFENAGNAYDMVFAAINLSKIGIPRYVSNFISEYFTKLGEYNNDYHDEPTLANVQEA
                     KAYALEQAYNNAYLQPGTYSFDSAYHIIVNDANLRNGAKFQDKSSLHHAEASYTHNFK
                     YNINLIGGLAFREYIPRSFGSIFHDTLVNRYDTLADGSPDLKAKFTKLNTYEVGAYTQ
                     VSVELFKKKLKLIGSLRIDKSQNFKIQVSPRLSAIFTQKNHTVRISYQQAFRNPTLQN
                     QYISLDLGAITLLGNLKGVYGYEANSVKAFQEAYQADPININFNLLKIIKYEKLRPEQ
                     VNSVEVGYRGAVKAKLYIDVSAYFSRYFHFIGDIRYYTPSADTVYVGTQPGADAMIDP
                     YYTKLFQQPVNARKKVDAYGASVGLNYYIWKSLVASFNYTYSGLNTKNLTDPIIPGFN
                     TPAHKFNIGLSAQRIWKGLGFAMNFKWVDKYMWESTFGDGIVPSYHTLDMNVSYEFEK
                     WFTLMVGGSNIYNNKHIEAYGSPKVGGLVYGSLLFDLDRNPNKKEKQR"
BASE COUNT         1448 a          902 c          871 g         1574 t
ORIGIN      
        1 gctgaagtgc ttcatatgta attatccagg gcgaaatagc tgtggcaaaa tttttaccca
       61 aaaacggtcc caggggctgg tactcccagc gttggatatc gcgggctgag aaatcattca
      121 acatgacggc accaaaaata tattctgttg cattttgtat atcaacaggt tcacccagct
      181 tattttcttt gccgattata taaccgagtt ctatctcaat atccaaagtc ttgcaagcag
      241 cataaacagg ggtctcgcct agtaaaattt gtccgcttgg gcgttttatc ggcgtattgg
      301 atgtgataat agatgaactt ctgccatgat atgcaatggg catgtgtttc cagttgggca
      361 tcagcggagg catagtgggt ctgaacattt tacccacgtt tgaagcatgt tcttctgatg
      421 cataaaaatc ggtataatcg cctaccttta ccggtacata catttttgca tcactttgtt
      481 tatctattaa gccatcataa gtgctaatga aatccggttc attttctagt aagtgttgta
      541 tggtgcttct cgtttgatga tgaatgcttt tccccaatgc tataaaatcg ttgaggctat
      601 cttgcaaaaa aacatccgtt tgaataaaag gaagttttcc ggatttgtat aagattgata
      661 aatcaataat aaaatcgcca atggccacgc cggctcgttt gatttttatt gcagtagaat
      721 aaataccgta aggcaagttg tgaacagtaa aatgcggatg ttgctgataa tttagcatgt
      781 tcgatttttt ttagttttca gaaattagcg cctgccagta ttcagcggct ctgcgtgtat
      841 gaggaatgca aatactgcca cctacgaggt tggctattgc gaaaatttca taaatctgtt
      901 cggtagaaat ttgtaaatca tggcatttgc ccaaatgata ttttatacaa tcatcacaac
      961 gcaataccat agaggctaca agccccagca tttctttagt ttttgcatct aaagcaccgt
     1021 ctgcataggc atgcatgtcg agattaaaaa agcggttgag cgtcaggttt ttttgttcaa
     1081 gaatgacgtc gttcatctta gagcgatagt cattaaattc ttttacgagt tcagccataa
     1141 aaaaagacta ttatctgcca aaagtacgac aaataatagt ctttgtaaat taattcggag
     1201 atccggaatt attttttctt tttagcagga gcttttttcg ctactgtatt ccagtcttcg
     1261 aataaatcga taccacggtt catagcatcc aatctgtcat aattcaaaga atagaagatt
     1321 tttttgccat ctctgcgtgt tttaaccacg tcaacgcctc ttaatacttt aaggtgttga
     1381 gaggtgatag actgctcaat acgaagtgtg ttgtagatta catttacgtt cacttctttt
     1441 ttgtcgttaa tcattttaat gattttcaga cgcagtggat gagcaactgc tttcagcagc
     1501 tccgctgctc tttcgatttt ttcggtgttt aattcgaaat tttccatata aattgtagtt
     1561 tagtttcaac aaaattataa aaaaattata atatcaaaac attttctgat tttttttctg
     1621 tgtagattaa tatattttca cacagataat tgatttatgt taaatttttc tgaaaaaatg
     1681 atatatttag tgtgcatatt tacacaaatg tgcataaaaa aattcccctt agtcgcaggg
     1741 gaatctcctt ttcagtgcta aaatcttaaa gaatctgcta attagttagt tagtctatat
     1801 taaaattggt tggtgtttct caaatgatga catattcact ccaaaatggc ttatcattaa
     1861 aatataaatt tcagcattaa aacgaacagc acgaattacc ttaccaattc gcctaacaaa
     1921 cggtttattt cttccatgcg gctttgatct acagaataat agatgtattt gccatctctg
     1981 gtagtaaaaa caactcctga acgacgcaaa agagctaaat gctgtgatgc tactgactgc
     2041 tccaggcgca tacgcacata caaatctgta acggtgattt tacctgtttc gtcaataatc
     2101 ctaatcattt cctgacgcat cttgtgattg atggcacgca gtaaaagcac tgattttctt
     2161 aaaacctgat aatcaatttt taactcaggt tgcatgatgg agttgatgtt tgctgacatt
     2221 tctttggttt tatttatttt caaatgttga gatttacgtt tatatagtgt aactatttga
     2281 aaatttggtt gcatcaaaat gcccaatttg tgtaaaatta atattaagta tctgtaaaat
     2341 acctaatttg atggggtgta aaaagcctta aaatagttgg gctcaaagta ggcaatatcc
     2401 tcaaactgcc gggctgtata tcgctgaaat gctaataaag actggtcttc agcactaatt
     2461 ataaaatcgt gaaaaagtag tatgtcttta aattcatcgg gaagttgcaa tttgttgata
     2521 ccgttgccga agaaaattat ttttttttcg caacccaacc cggccagcca ctcttcatca
     2581 agtattaaag acgaaaatga tttttcagct ttcaagtctt gattatagta tgttgtaaat
     2641 acttccattc ttcgggcatc tatcatcgga atataccaat cataacccgt ttgtccatat
     2701 cgatgaatac aaccatacgc cattgccttt aacgtgggaa tggcaatcaa gggaagattt
     2761 tgtgcaaagc atatcccttt tgacatggca gcaactatac gcaatccggt atatgaaccc
     2821 ggaccactgc tcacagacac ggcgtgtata tcgctaaact tgagcttatt attatccagt
     2881 atctgttgca ccatactgtg tgctacatca gcagtctgat tgctttgcgc tgagaaaagg
     2941 acatccgtgc aaatgccatt tttgctaatg gcaaatgacg tgttcccata agcagcttcg
     3001 atatgtaata gcaaaacatc aggcatctga ctgcaaaaat aaaaaattaa ttgcgttgaa
     3061 ataacggcta tttaagatag ctgatgctgt agtgtgtcac gatttccgtg acaaatctaa
     3121 ttacctatcg ttgtttctct ttcttattcg gattcctgtc caaatcaaac aacaaactgc
     3181 catataccaa ccctcctacc ttaggcgagc cataagcttc gatatgtttg ttgttgtaaa
     3241 tattagaacc accgaccata agcgtaaacc atttctcaaa ttcgtaactg acattcatgt
     3301 ccagtgtgtg ataactgggg actatgccat cgccaaaagt actttcccac atgtacttat
     3361 ctacccattt aaaattcatg gcaaatccta atcctttcca gattcgctgt gcactcagtc
     3421 cgatattaaa tttgtgagcc ggtgtattaa aacctgggat aatgggatct gtcagatttt
     3481 ttgtattcaa gccggaataa gtataattaa acgaagccac caatgatttc cagatataat
     3541 aattaagtcc gacagatgcg ccgtaggcat ccaccttttt gcgtgcattc acaggttgct
     3601 ggaacaattt ggtataatat gggtcaatca tcgcatcagc acctggctgt gtgcccacat
     3661 aaacagtatc agccgaaggc gtataatacc gtatatcacc gataaagtgg aaataacggc
     3721 tgaaatatgc acttacatct atatacaatt tggctttcac agcacctctg tagcccactt
     3781 ctactgagtt cacctgttca ggacggagtt tctcgtattt aataatttta agcaaattaa
     3841 agttgatatt aatcggatcg gcttgatatg cttcctgaaa tgcttttaca gaattagctt
     3901 catatccata tacgcctttt aaattaccaa gtaatgttat tgcacccaaa tcaagtgaaa
     3961 tatactgatt ttgcagagtc ggattgcgga aagcttgttg gtaagagatg cgtaccgtat
     4021 gatttttttg tgtaaatatt gctgacaaac gaggagatac ttgtattttg aaattctggc
     4081 ttttatcaat cctcaaagaa cctattagtt tcagcttttt cttaaacagt tccacactta
     4141 cttgtgtata tgcccccacc tcgtaggtgt tgagtttggt aaatttagcc tttaagtccg
     4201 gagaaccatc agcaagcgtg tcatatctgt ttactaatgt atcgtggaag atagaaccaa
     4261 aagagcgagg aatatattcc cggaatgcca aacccccaat caggttaatg ttatatttga
     4321 aattatgtgt gtaagatgct tctgcatgat gcaaagatga tttatcctga aattttgctc
     4381 catttcttag attggcatca ttgacaataa tatggtaagc agaatcaaag ctgtatgtac
     4441 cgggttgcag ataagcatta ttgtaggcct gttcaagtgc ataagcttta gcttcctgta
     4501 cgttggctaa agtaggctca tcatgatagt cgttattata ttcgcccagt ttagtaaaat
     4561 attccgatat aaaattagat acatagcggg gtatcccaat cttagataaa ttaatagccg
     4621 caaataccat atcataagca ttgcctgcat tctcaaatgt agaataatat ttcagtgaaa
     4681 gtcctttata atctacttgt aatttgggtt gatgaaaagt aatatttttc acattatagc
     4741 gattggctcc ctgataaaca gcactgccca tgccaaactt atataataat tctat
//
LOCUS       DAOQII010000199         4763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10610_length_4763_cov_4.984707, whole genome shotgun sequence.
ACCESSION   DAOQII010000199 DAOQII010000000
VERSION     DAOQII010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10610_length_4763_cov_4.984707"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1222
                     /locus_tag="PLF48_09165"
     CDS             32..1222
                     /locus_tag="PLF48_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNF49537.1"
                     /translation="MLARIINNIKGDKYVWYIIAALSSISLLLVYSATRSLAYKNMHG
                     NTEYYLIRHGFMLFVGLFIIYIVHQIDHRYFSRIAQMMLILSVPLLLYTKFFGVRINE
                     AARWVRIPVIGLTFQSSDFAKLALIMYTSRMLSKKQGVIKDIKEVTLHIMLPIVVICG
                     LIMIDNFSSAGILLMTCIVVLFIGRVSTKFILAIGGAGVVMIGLIFAFSWFLLPDHGR
                     ADVWVQRIENWIKPDVKSDDFYQQKQAYIAIAKGGLVRVAPGKSTQSNFLPDAFSDFI
                     YATLIEEYGLLGGAVVLFLYLFLMYRLILLVKDSPRAFGALMAVGLGVSMVLQAIIHM
                     GVNVHLLPNTGITLPLISWGGTSIWFNAFALGIILSVSAYVDERKQDKSGKKKDAIID
                     EIHG"
     gene            1502..2626
                     /gene="murG"
                     /locus_tag="PLF48_09170"
     CDS             1502..2626
                     /gene="murG"
                     /locus_tag="PLF48_09170"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNF49538.1"
                     /translation="MKTQTKHKGRFIISGGGTGGHIFPAVAIAEAIKQKMPDAAILFV
                     GAVGRMEMEKIPLAGYDIVGLEVMGLERKFTLKNFSILFKMIKSFFKARKIIKDFQPD
                     ACIGVGGYVSASVLFVASMMGIKSFIQEQNSYPGITNKILSKFASKIFVAYDNLADFF
                     PKDKLVLTGNPVRQDICGVLPDKNAALDFFGLNQTKKTILIIGGSLGARTINNAIEQH
                     LDVYTQAGYQLLWQTGKGYYDGIIERTKNKDLNAVHIHQFIKEMNYAYAAADVIISRA
                     GALSISELCIVGKPCILIPSPNVTEDHQTKNAMALVNKQAALLITDKEADEKLANAVL
                     NLLNNKNEQEQLGVNLKQLAKTNAAEDIVNKIFKEINDER"
     gene            2627..2998
                     /locus_tag="PLF48_09175"
     CDS             2627..2998
                     /locus_tag="PLF48_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF49539.1"
                     /translation="MMIENKDILKSKSFSFAVKIINLYKILTVEMKEFVISKQILRSG
                     TSVGANVRESKNAESAMDFIHKLSIAQIECDESLYWLELLYTTEFINKEMFDSLSNDA
                     TEILKIIRSSILTKKKNLKRN"
     gene            3104..4474
                     /gene="murC"
                     /locus_tag="PLF48_09180"
     CDS             3104..4474
                     /gene="murC"
                     /locus_tag="PLF48_09180"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNF49540.1"
                     /translation="MINLHKLKNIYFLGIGGIGMSALARYFNLHGKTVKGYDKTPTTL
                     TDELIAEGMHITFDDSLETLDKNADLVVYTPAIPKDSIQLNYYLQSGIPMQKRAQVLG
                     AVADEAFNISIAGSHGKTSTSSITAHILKSANKDVAAFLGGICINFNSNFINGTAYAI
                     AEADEFDRSFLNLHPNIALITSVDTDHLDIYGNLKAIEDSFSLFCENIRNNGVCIANA
                     TVKQEILNQKVKNLRYSLHDRSADFYAENIRIENGSYIFNVIHPDGKLVDIVSYYGGR
                     HNIENAIGAIAIALEVGVNETDIRNAIETFRGVKRRFETHIKNEKHIYIDDYAHHPRE
                     LDAALLSAKELYPDKKITAAFQPHLFSRTRDLCDEFAASLSKIDQLILLDIYPAREKP
                     IEGVTSKIIFDKVTIADKHLMTKNELTDFIRNHKDDIEVLLTVGAGDIDTKVREITEI
                     LNEPMN"
     gene            4539..>4763
                     /locus_tag="PLF48_09185"
     CDS             4539..>4763
                     /locus_tag="PLF48_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49541.1"
                     /translation="MNAATKNIIFRISILLGLVLFVFLVVNTIIHRKSTLIKKTEITI
                     DDLDGNFMLDKKQISALLNKNFEIRNNKLNG"
BASE COUNT         1627 a          735 c          944 g         1457 t
ORIGIN      
        1 atctttctcc ctaatcacca atcactaaaa aatgctcgct cgcatcatca ataacatcaa
       61 aggagacaaa tatgtgtggt atataattgc cgcattgagt tctatttctc tgttgttggt
      121 atatagtgct acgcgttctt tagcatataa aaacatgcat ggcaataccg aatattattt
      181 gatacgtcat ggctttatgt tgtttgtcgg tttatttatt atctatatcg tacatcagat
      241 tgaccatcgc tatttttcca gaatagcgca gatgatgctg attttgtcag taccattatt
      301 gttatatacc aagttttttg gtgttcggat aaacgaagcg gcacgttggg tgcgcattcc
      361 tgtaatagga ttaacctttc aatcttcaga ctttgccaag ctggcattaa tcatgtatac
      421 ttcgaggatg ttgtcaaaaa aacaaggtgt tattaaagat atcaaggaag taacattaca
      481 tatcatgttg ccgatagtcg tcatttgtgg tttgattatg atagataatt tttcgagtgc
      541 cggcatttta ttaatgacat gcattgtggt tttgtttatt ggtcgtgtaa gtacaaaatt
      601 tatactggca atcggaggag ccggtgtagt gatgataggg ttgatatttg cattctcctg
      661 gtttctgtta cccgatcatg gcagagcaga cgtatgggtg cagcgaatag aaaactggat
      721 aaagcccgat gtaaaatcag atgattttta ccagcaaaaa caagcctata ttgccattgc
      781 aaaaggtggt ttggtacgtg ttgcaccggg caagagtaca caaagcaatt ttttaccgga
      841 tgctttttcg gattttatct atgctacact tattgaagaa tatggattgc tgggcggagc
      901 tgtagtattg ttcctctatt tatttttaat gtacaggctg atattattag ttaaagattc
      961 accgcgcgcc ttcggtgcgc tcatggctgt cggcctggga gtcagcatgg tcttgcaagc
     1021 catcattcac atgggtgtaa atgtgcatct gttgcctaat accggcatca cacttccatt
     1081 aatcagttgg ggcggaacaa gtatatggtt taatgcattt gcattaggta tcatccttag
     1141 tgtaagtgct tatgtagatg aacgcaaaca agataaatca ggcaagaaaa aagatgccat
     1201 tatagatgaa atacatggtt gatagaaatg ataaataatg aatgataaat aatgaattat
     1261 aaatgatgaa tgttgaatgt ttaatgatga ataaaagaaa atagttgcca cgaagtaagt
     1321 cccctgttaa ggagatttag gggtgaagta aaataatgaa ttataaatga tgaatgttaa
     1381 atgtttaatg atgaataaaa gaaaatagtc gccacgaggt aagtcccctg caaaggggat
     1441 ttaggggtga aacgaaacga tgaaagatga acaaaatttg aaactttaga agatattaat
     1501 tttgaaaact caaacaaaac ataaaggcag atttataatt tccgggggtg gaacaggtgg
     1561 acatattttt ccggctgtcg ctattgcaga ggctatcaaa caaaagatgc ctgatgcggc
     1621 tatccttttt gtgggtgccg tcggcagaat ggaaatggaa aaaattccat tggcaggtta
     1681 tgatattgtt ggtttagagg taatgggact tgagcgaaag ttcactctga aaaatttcag
     1741 cattctgttt aaaatgataa agagcttttt caaggcaaga aaaattatta aagactttca
     1801 gccggatgcc tgtattggcg taggcggtta tgtgtcagca tctgtgttgt ttgtcgcttc
     1861 gatgatgggc atcaaatcct ttatacagga acaaaattca tatccgggca ttaccaataa
     1921 aatactgtca aaatttgcca gtaagatttt tgtagcctat gataacttag ctgatttttt
     1981 cccaaaagac aaactggttt taaccggaaa tccggtaagg caagatattt gtggggtact
     2041 ccctgataaa aatgcagctt tagatttctt tggtttaaat caaactaaaa aaacaatctt
     2101 aatcatcggt ggaagtttag gtgcaagaac tatcaataac gccattgaac aacatttgga
     2161 tgtatatacc caagcaggtt atcagttgtt gtggcaaacc ggaaaaggat attacgatgg
     2221 tattattgaa cgaactaaaa acaaggatct aaacgcagtg catattcatc agtttataaa
     2281 agaaatgaat tatgcctatg ctgcagcgga tgtcattatt tcgcgtgcgg gtgcattgtc
     2341 tatttcagaa ttatgtatcg tcggcaagcc ttgtatttta attccatctc cgaatgtaac
     2401 cgaagatcat caaacaaaaa atgcaatggc attggtcaac aaacaagcgg ctttattaat
     2461 tacagataaa gaggctgatg aaaaattagc aaatgcagtg ttgaatttat tgaataataa
     2521 aaatgaacaa gagcaactag gtgttaactt aaaacaatta gcaaaaacaa acgctgcaga
     2581 agatattgtg aataagattt ttaaagagat aaacgatgaa cgataaatga tgattgaaaa
     2641 taaagatata ttaaaatcaa aatccttctc atttgcagtt aagattatta atctatataa
     2701 aatcctgaca gttgaaatga aagagtttgt aattagtaaa caaatattgc gttcaggtac
     2761 atcagttgga gctaatgttc gcgaatctaa aaatgcagag agtgcaatgg attttattca
     2821 taagttatca attgctcaaa tagagtgtga tgaatcatta tactggttgg aattgttata
     2881 tactactgag tttattaata aagaaatgtt tgattcactt tcaaatgatg caacagaaat
     2941 tttaaaaatt attagaagct caatattaac aaagaaaaaa aacttgaaaa gaaattgaaa
     3001 aaactaatta ttaagccaaa tacagaatca gttaaaacca ataggtttta ttgtgtacca
     3061 tatattattc ataattcatc tcttatcgtt tatagttcat acaatgatta atttacataa
     3121 actaaaaaac atctacttcc tgggtattgg cggcattggc atgtcggcac tggctcgtta
     3181 ttttaatttg cacggaaaaa cagtgaaagg ttatgataaa acgccgacaa cacttacgga
     3241 tgaattaata gcagaaggta tgcatatcac gtttgatgat agtttggaaa ccttagacaa
     3301 gaatgctgat ttagttgttt atactccggc aattcctaag gacagtattc agctcaatta
     3361 ctatttgcaa agcggtattc ccatgcaaaa acgggcgcag gtattagggg cagttgcaga
     3421 tgaggctttc aatatttcta ttgcaggctc gcatggcaaa acatctactt cttccataac
     3481 ggcgcatatt ttaaaatctg ccaacaaaga tgtcgctgca tttttgggcg gaatttgtat
     3541 caacttcaat tctaatttta tcaatggtac tgcttatgca attgccgaag cagatgaatt
     3601 cgaccgatcg tttttaaatc ttcatccaaa cattgcgttg attacttcgg tagatacaga
     3661 tcatctggat atttatggca acctgaaagc tattgaagat tctttcagtt tgttttgtga
     3721 aaatatccgc aacaacggag tatgtattgc taatgcaaca gtgaaacaag aaatattaaa
     3781 tcagaaggtg aaaaatctgc gctatagtct gcatgatagg agtgctgatt tttatgcaga
     3841 aaatattcga atcgaaaatg gaagctatat tttcaatgtc attcatcctg acggaaaatt
     3901 agtagatatc gtatcgtatt atggcggacg gcataacata gaaaatgcca tcggtgccat
     3961 agccatagcc ctggaagttg gtgtgaatga aacagatata cgaaatgcga tagaaacatt
     4021 cagaggtgta aaacgtcgtt ttgagacgca tataaagaac gaaaagcata tttatataga
     4081 cgattatgcg caccatcctc gtgagttaga tgcggcattg ctttctgcaa aagaactgta
     4141 tccggataaa aaaataacag ccgcttttca gccgcacttg ttttcacgta cccgcgattt
     4201 atgtgatgaa tttgcagcat cgctatctaa aatagatcaa ttgattttgc tggatattta
     4261 tcccgcaaga gaaaaaccga tagaaggcgt aactagtaaa ataatttttg acaaagtaac
     4321 catagcagat aaacatttaa tgacaaagaa tgaacttacc gattttatac gaaatcataa
     4381 agacgatata gaagtattac ttaccgttgg tgccggcgat atcgatacca aagtgagaga
     4441 aattacagaa attttaaatg aaccaatgaa ttaatatagc aatttaccaa tgactaattt
     4501 accaatgacc aatacctaaa agctaaatct tacaaatctt gaacgcagct actaaaaata
     4561 tcatcttccg catttccatc ttattgggat tggtattgtt tgtgtttttg gtagtaaata
     4621 caattattca tcgtaaatct acgctgatta agaaaacaga aattacgata gatgatttag
     4681 acggaaattt tatgttggat aagaaacaaa tcagtgcatt actcaataaa aattttgaaa
     4741 tcagaaataa caagctgaat ggg
//
LOCUS       DAOQII010000200         4759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10627_length_4759_cov_4.734269, whole genome shotgun sequence.
ACCESSION   DAOQII010000200 DAOQII010000000
VERSION     DAOQII010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10627_length_4759_cov_4.734269"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PLF48_09190"
     CDS             <1..781
                     /locus_tag="PLF48_09190"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNF49542.1"
                     /translation="DMSNDAIGYAVENMEMVNKTLNGLLKKYSTTGKSYHELRNAYLT
                     LTGNYNRSLNVVNRYIGGVYINRNMVGQDSLSKPYIPVSLADQKRAMQTLVKYAFAVN
                     AFKTPQDLYNYLQQQRRGNERKENEDPKIHDRILNIQKSILDELLNGAVMQRLIDTKL
                     YGNTYSLNMMLEDLTNGIFTEDLNSAVSSLRQNLQIEYVNRILAVMDNKSAYANNIKS
                     AAFGEADKIKTWMKMNPGIDASTKSHRKYIGFLIDKAMKLD"
     gene            853..1752
                     /locus_tag="PLF48_09195"
     CDS             853..1752
                     /locus_tag="PLF48_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNF49543.1"
                     /translation="MLLRVLKTYIASNLFIATAAVAMMWVSCIWLHIPLFHSPWLIIE
                     VFFSTWFIYQFSRYIYYKKGIFTDESELVLQSFKQYPLLNLWSIVISALLSAIAFFFL
                     HTTTKAGLIFTGLISILYPAPIGKLWSSNMRLRDIPIAKIFLIMLVWSVSCVLLPVWE
                     NGISLNERKDVYILFLIQCVFLLFITLPFDIHDYATDKQAELKTIPVLTGIRKAKWIC
                     FLLGALYVIGLLYLFMLENWRSLASIYLSETGILLIILLVILLQVFTFYRSDKTEKWI
                     IKLVYDGSMLLYFFIMILTCKTA"
     gene            1749..2528
                     /locus_tag="PLF48_09200"
     CDS             1749..2528
                     /locus_tag="PLF48_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNF49544.1"
                     /translation="MNSFGLIETMKVANGRMVFKNLHIERLHQSLKTINIEESIFKLE
                     DRLLRLMQYECIAHGLKNFRLRVEVQKSNIKEYAKDFSGLKWNCTIKQSDDIRYKWNE
                     NGLSLTILPKYQKQTDLFSNLKLTERSIYIKAQAYAASTGFDHALVLNENNTIADADI
                     YNVFVVKDDIIYTPSLACAPVVGVFRRLLLEKIPQLQIIEKPISVAEIYQADELFLTN
                     AFRGIQWVNQLDEKYFTPTKTRAVFERVSQFISTHFGEHLV"
     gene            complement(2578..3000)
                     /locus_tag="PLF48_09205"
     CDS             complement(2578..3000)
                     /locus_tag="PLF48_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF49545.1"
                     /translation="MKRREPVSHIMTKEVISVDYTASLKDIKHIIEDKHIRHIPVLNG
                     NKVVGIISKTDYLQLTLGAVYPNQEDANEALMEMFKLEDLMVKNPICVQPDTTIREVT
                     EIFILNEFHALPVTENDKLVGIVSVKDVLKFLDELYYN"
     gene            3149..3550
                     /locus_tag="PLF48_09210"
     CDS             3149..3550
                     /locus_tag="PLF48_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNF49546.1"
                     /translation="MTALEIVKHYYDNFNNKNWQGMMELLSDDIRHDANQGATYFGKE
                     NFEKFLQKMDECYDEELKDLVLFQATLTERIAAEFTVHGYYKKTDCDLPPAKNQHYIL
                     PAGAFLEIQNGKITRITTYYNLPLWISLVSA"
     gene            3526..4125
                     /locus_tag="PLF48_09215"
     CDS             3526..4125
                     /locus_tag="PLF48_09215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49547.1"
                     /translation="MDQLSFSIKHGKAILSVLDDLAALRIRIFRAFPYLYEGTIDYEL
                     TYLQTYTDCPQSFLFCVYDGNKMIGATTCIPLVYETKEVQLPFIKSGMDIANIMYYGE
                     SMLDETYRGRGIGKLFFEEREKYSTSLKSINTACFCAVERPDNHPLKPDYYTPHDIFW
                     TKMGFSKNPDIFSCFEWQDLHEDSPTSKKMVYWFKKITK"
     gene            4138..>4759
                     /locus_tag="PLF48_09220"
     CDS             4138..>4759
                     /locus_tag="PLF48_09220"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF49548.1"
                     /translation="MRHFIFILILFPLLSYAQTGVIEGRVYDEINNEPIIGANIGIKD
                     STIGTSSDLDGNYRIEGLKAGLYNIEISYLGYLTVVKYEIQVFNSKPVRLDVAMKLSA
                     AMIDSVVVSAKAFSKKEETPLSLISIGVNEIQRNPGGNRDISRSIKNLPGVSSGVGFR
                     NDLLVRGGAPNENRFYLDDIEIPTINHFQTQGASGGSNGLLNVDFIN"
BASE COUNT         1560 a          842 c          860 g         1497 t
ORIGIN      
        1 tgacatgagt aatgatgcaa ttggttatgc cgttgaaaat atggaaatgg tgaataaaac
       61 actaaacgga ctcttaaaaa aatacagtac taccggaaaa tcttatcacg aattgcgcaa
      121 tgcttatctg acacttaccg gaaattacaa tcgttcgctg aatgttgtaa accgctatat
      181 cggtggcgta tacatcaacc gaaatatggt ggggcaagac agcctcagca aaccatatat
      241 ccctgtttca ttagccgatc aaaaacgtgc tatgcagact ttagtgaaat atgcttttgc
      301 tgttaatgct tttaaaacac cacaagattt atataattat cttcaacaac aaagacgggg
      361 taatgaacgc aaagaaaatg aggatccgaa aatacacgat cgcattttaa atattcagaa
      421 atccatactg gatgaattat tgaatggagc tgttatgcaa cggcttattg atactaaatt
      481 atacggcaat acgtacagct tgaatatgat gctggaggac ttaacaaatg gaattttcac
      541 agaagactta aacagcgctg taagtagttt gcgtcagaat ttacagattg aatacgtcaa
      601 ccgaatatta gcagtcatgg acaacaaatc tgcctatgcc aataatataa aatctgcagc
      661 atttggcgaa gccgataaaa taaaaacatg gatgaaaatg aatcccggta tagatgcatc
      721 aactaaatca catagaaaat atatcggctt cctgattgac aaagccatga agctggatta
      781 aagtaacttg atattttact tacacttaaa ttactgctcc ttacagcagc taattttatt
      841 gtatattcgg ttatgctttt gcgtgtttta aaaacttata tcgcatccaa tttatttatt
      901 gccacagctg ccgtggcaat gatgtgggta agttgtatct ggctgcatat tcctttattt
      961 cattcgcctt ggctgattat tgaagtcttt ttttcaacat ggtttatcta tcaatttagc
     1021 cggtatatct attacaaaaa gggcatattc accgatgaat cagaactggt attgcaatcg
     1081 tttaaacaat atcctctact caatttatgg tctatcgtca taagtgcatt actatcagcc
     1141 attgctttct tttttctgca tacaactacg aaagcaggac taatcttcac aggattaatt
     1201 tccattttat atcctgcacc aataggcaaa ctttggtcaa gcaatatgag gctcagggat
     1261 attccaatag cgaaaatatt tttgattatg ctcgtttgga gtgtaagctg tgtactgtta
     1321 cctgtatggg aaaatggcat ttctctgaat gagcgcaaag atgtgtatat actctttttg
     1381 attcagtgtg tgtttttact ttttattact ttgccgtttg atattcacga ctatgcaacg
     1441 gacaaacaag ccgaattaaa aacaattcct gttttaacag gtattcgcaa ggcaaagtgg
     1501 atttgctttc ttttaggagc actttatgtt atagggttac tttatttatt tatgcttgaa
     1561 aactggcgca gtttagcatc tatttatttg tcagaaaccg gtattctttt aatcatactg
     1621 cttgttatat tattgcaggt ttttactttc tatcgttcgg ataaaactga aaaatggatc
     1681 atcaaattag tttatgacgg aagtatgctg ctctattttt tcatcatgat tttaacttgt
     1741 aaaacggcgt gaacagcttc ggactgatag aaacgatgaa agtggcgaac ggcagaatgg
     1801 tatttaaaaa tctgcatata gagcgtttgc atcaaagtct gaaaacgatt aatattgaag
     1861 aatcaatttt taaattagaa gatcgactat tgagattaat gcaatatgaa tgtattgctc
     1921 atggactgaa aaacttcagg ctgcgtgtgg aagtgcagaa aagcaacatt aaagaatatg
     1981 caaaagattt cagtggacta aaatggaatt gcaccatcaa acaaagtgac gatatacgat
     2041 ataaatggaa tgaaaacgga ctctcactca ccattcttcc taaatatcaa aaacaaacag
     2101 atttattttc caatttaaaa ctgaccgaaa gaagcattta cataaaagca caagcatatg
     2161 ctgcctccac aggatttgac catgccctgg ttttaaacga aaacaatacc atcgcagatg
     2221 cggatattta taatgtcttt gtggtgaaag acgatatcat ttatacacct tctttagcat
     2281 gcgctccggt agtcggcgtt ttcagaagat tattactgga gaaaataccc caactacaga
     2341 ttattgaaaa gccaattagc gttgcagaaa tttatcaggc tgatgagcta tttcttacta
     2401 atgctttccg aggcatccag tgggttaatc aattggacga aaagtatttt acacccacaa
     2461 aaacgagagc tgtatttgaa agagtttccc aatttatatc cacacacttt ggcgaacatc
     2521 tggtataaaa aaaagcttcc gtttccggaa gccctctctt caatttgtag ttggtggtta
     2581 attataatag agttcgtcta aaaatttcaa gacatccttt acggatacta taccgaccag
     2641 tttatcgttt tccgtaacag gcaacgcatg aaactcattc agaataaaaa tttcagttac
     2701 ttctctgata gtagtatccg gttgcacgca aataggattt tttaccatta agtcttccag
     2761 tttaaacatc tccataagtg cctcattggc atcctcctga ttgggataca ctgcgcctaa
     2821 agtcagctgc aagtagtcag ttttactgat gatgcccacc actttatttc catttaatac
     2881 cggaatatgg cgaatgtgtt tatcttcaat gatatgtttt atatctttta atgatgcagt
     2941 gtaatctacg ctgattactt ctttagtcat aatatgggaa acaggttctc ttcttttcat
     3001 gattttattt tttacaaatt taaatcgtaa attatttaag tgtattgaca aacttcatca
     3061 ccttatgtga tttttatcaa ggcaaatatt attatattaa ttccggcttg cagaaattat
     3121 acaagtttct aactttgcag aaaaaatcat gacagcactg gaaattgtaa agcactatta
     3181 cgataatttc aataataaaa actggcaagg catgatggaa ctgctatccg atgatatcag
     3241 acatgatgct aatcagggag ctacttattt tggaaaagaa aactttgaaa aatttcttca
     3301 aaaaatggac gaatgctatg atgaagaact caaagatttg gttttgtttc aagccacttt
     3361 aacagaaaga atcgctgcag aatttactgt acatggttat tataaaaaaa cagactgcga
     3421 tttacctcct gccaaaaacc agcattatat acttcctgca ggtgcatttt tagaaattca
     3481 aaacggaaaa atcacccgca ttacgaccta ctataatctg ccattatgga tcagcttagt
     3541 ttcagcataa aacacggcaa agccatactt tcggtattgg atgatttagc agcattgcgc
     3601 attcgcattt ttcgtgcttt cccctacttg tacgaaggca ctatagatta tgaactaacc
     3661 tatttacaaa cctatacaga ctgcccgcaa tcttttttat tttgtgttta tgatggcaat
     3721 aaaatgatag gtgctaccac ttgtatacct ttggtttacg agacaaaaga agtgcaactg
     3781 ccttttataa aatccggtat ggatatagca aacattatgt attacggaga gagtatgctg
     3841 gatgaaacat atcggggaag aggtataggc aaactctttt ttgaagaaag ggaaaaatac
     3901 agtacgtcat taaagagcat aaatacagcc tgtttttgcg ctgtagaaag acctgacaat
     3961 caccctttga agcctgatta ttacactccg catgatattt tttggacaaa aatgggtttc
     4021 tcaaaaaatc cggatatctt ttcctgtttt gaatggcaag atttacatga agacagccca
     4081 acttcaaaaa agatggttta ttggtttaaa aaaataacta aatagaacat tcaatttatg
     4141 agacacttca tctttatttt aattctattt cccttattaa gttatgccca aaccggagta
     4201 atcgaaggca gagtttatga tgaaattaat aacgaaccaa taattggagc taatattggt
     4261 ataaaagatt ccaccatcgg cacctcatct gatttggatg gcaattatcg catcgaagga
     4321 ttgaaggcgg gtttatataa tattgagatt tcgtatttgg gctacctcac tgtagtaaaa
     4381 tatgagattc aggttttcaa ttccaagccc gtacgtctgg atgtggccat gaaactgagt
     4441 gcagcaatga tagattctgt tgttgtatca gcaaaagcat tcagcaaaaa agaagaaacc
     4501 cctttatctc tcatcagcat tggtgtaaat gaaattcagc gaaacccggg tggcaacaga
     4561 gatatttcgc gttctataaa aaacctgccg ggtgtttcat caggtgtggg cttccgcaat
     4621 gatttgctgg tacgaggcgg tgcacctaat gaaaatcgtt tttatttaga cgacatagaa
     4681 attcctacta tcaatcattt tcaaacacaa ggcgcaagcg gtggaagcaa tggcttactt
     4741 aatgtagatt tcatcaata
//
LOCUS       DAOQII010000201         4734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10698_length_4734_cov_4.770891, whole genome shotgun sequence.
ACCESSION   DAOQII010000201 DAOQII010000000
VERSION     DAOQII010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10698_length_4734_cov_4.770891"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /gene="feoB"
                     /locus_tag="PLF48_09225"
     CDS             <1..1892
                     /gene="feoB"
                     /locus_tag="PLF48_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNF49549.1"
                     /translation="ITSQVLTNLHDKDYPDVIVYIADVTHLERHFLLLTQLMDLDFPI
                     ILALSMNDVAEQRQLTCDENVLKKKLGIPVVKINSRKSTGIKELKEELFNLLQSGESS
                     NKLQLKKWQASELQLATAIADELPVKNNYHAFLLAHHLSKNSFLADHEKQKINQLIQE
                     NNTDILSAQLEEIMERYNAFTPIVNASLRRTRQHAQTLSDKIDNFITHPILGPILFFL
                     ILFLFFQAIFSWSVVPMEAIDIFFSRIAEKLHTTLPDSIFSRILTEGIIPGISGVLVF
                     VPQISILFFFIAVLDEIGYMSRAIFLFDNIMLKFGLNGRSIVSLISGGACAIPAIMSA
                     RTIGNWKERLITIMVTPWISCSARIPVYAILIGLVIPVKKIAGFNLQGIVFMLLYLTG
                     IFAALSAAFVFKKVLKTEEPSFLMMELPDYKFPHWKNVVLNVYDKVKSFVLGAGKIIL
                     IVSVLLWFMASFSPFPTKTDHLIKEEIAAKNFSNPTELQHFNEEKKLEYSYAGYMGKM
                     IEPVIKPLGYDWKIGIALITSFAAREVFVGTISTLYSIGKDASDLTIKQKLAKEKKED
                     GTAVFSFATAISLLIFYVFAMQCMSTLVTVNRETNSWKWPIIQLIFMTSTAYICSFLV
                     YHILR"
     gene            complement(1919..3541)
                     /locus_tag="PLF48_09230"
     CDS             complement(1919..3541)
                     /locus_tag="PLF48_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF49550.1"
                     /translation="MKRLFTLLVFFAVLHVQAQLLRGPYLQSMTNKSVKILWRTAAAT
                     PAVVRYGTALNNLTNSVADTTQKTNHILQISGLIANTKYYYQIEDGNMQILAGNDEQH
                     HFFTAPVIGDSTIHRFWVTGDFGAGNTDQVLVKQWFQNYLTTTKVDAWLWLGDNTYTN
                     GTDAEYSRNVFSKDYGYDSLFRFLPFYPIPGNHDYNSVNGSNPVNPYGPYFDMVEVFK
                     NAEMGGYPSNTEAFYSYDYGNIHFVAMNSELYPWLLFNLPNAYKTWLENDLRNTTQPF
                     KIAYWHQPPYSKGSHDSDDWFEFFMKAMREQYLPILERYGVDLVLNGHSHVYERSYLI
                     NKHYGLSPTFKRSTMMVDSTSGNPDSNRMYIKYTYGVNKNKGTVYAVVGNSGKSEAEN
                     NKMHPVMYKKYAASGGVGSMILEVSGHTLVCKYYKANGELLDKFSIVKMDSTVISAIK
                     ENKLINQMQVYPNPFNQSLTVDFSAQKSGEISIYIQNITGQLLTQKLWDGNSKAGANS
                     VFIDHLDELPKGEYIISITQNGEILSKTIVKL"
     gene            complement(3538..>4734)
                     /locus_tag="PLF48_09235"
     CDS             complement(3538..>4734)
                     /locus_tag="PLF48_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNF49551.1"
                     /translation="RGKQKIISGDRHQMPPSAYFASEVVFYEQQTEEESEVENFLAES
                     NSLLEYADSSDFINTYLSFHYRSEHPDLIRFSNAAFYDNRLIPMPETHAYQPLFLHQI
                     NGVYGEGLNESEADAITDFVFRDENMLADKSVGIATFNIFQRDLILDKLYEKAAQSKE
                     NQDKLNALLQNGLFVKNLENIQGDERDIILISTTFGKDASGKFRQFFGPLSTDKGYQL
                     LNVIITRAKKQLHVFTSFPEEYFTQYETELKQKGNTGKAIVYAFLSYVKWCDKQETEI
                     KTGLLEALVINKNAIQQQNINAAYKNEIEQYLHQHFSNYTIVKNMPFGGFILDFVLKK
                     GEYIVLCIDLENDLPNHAATNYRLKLHKEKMLNKFQIPVFHLWLYDFWKNKDSLEIEI
                     KNKIKS"
BASE COUNT         1394 a          916 c          855 g         1569 t
ORIGIN      
        1 gcattacatc tcaggtctta accaatctgc acgacaaaga ttatcctgat gtaattgtct
       61 atatcgctga tgttacccat ttggaaaggc atttcttatt acttacccag ctgatggatt
      121 tggatttccc tattatactt gcccttagca tgaatgatgt ggcggaacaa agacaactga
      181 cctgtgatga aaatgtatta aaaaagaaat tgggcattcc ggttgttaaa atcaacagca
      241 gaaaatcaac cgggataaaa gaattgaaag aagaactgtt caacttgtta cagtcgggtg
      301 aatcatccaa caaattacag ctaaaaaaat ggcaagctag cgagttacag ttagccacgg
      361 caattgctga tgaattacct gttaagaaca actatcatgc atttttgctg gcacatcacc
      421 tcagcaaaaa cagtttttta gctgatcatg aaaaacaaaa aataaatcag cttattcagg
      481 aaaacaatac cgatatttta tctgcacaac tagaggagat tatggagcgt tataatgctt
      541 ttactccgat tgtaaatgct tccttaagac gcacccgaca acatgcccaa actttgtcag
      601 acaagattga taattttatc acccatccga ttttgggacc tattttattt ttcttgattt
      661 tgtttctttt ttttcaggct atattttcct ggtcggttgt tccgatggaa gccattgata
      721 tctttttttc aagaatagca gaaaaactac ataccacgtt gccggattct attttctcac
      781 gcattctgac agaaggtatt ataccgggca ttagtggtgt actggttttt gttccgcaga
      841 ttagcatctt gttctttttt attgccgtgt tggacgaaat cggatatatg tcgagagcca
      901 ttttcttgtt tgacaatatc atgctgaagt ttggactaaa cggccggagt atcgtatcac
      961 tgatatcagg cggggcttgt gccatacctg ctatcatgag tgctcgtact attggcaact
     1021 ggaaagaaag acttatcacc attatggtta ccccatggat tagttgttcg gctcgcatac
     1081 ctgtttatgc cattttaatc ggcttggtta ttccggtaaa aaaaattgca ggcttcaact
     1141 tgcaaggcat agttttcatg ttgctttatc tgacaggcat ttttgcggct ttgagtgcag
     1201 catttgtttt caaaaaagta ttgaaaacgg aagagccttc ttttctgatg atggaactgc
     1261 cggattataa atttccgcat tggaaaaatg tagtgttgaa tgtgtatgat aaagtaaaat
     1321 catttgtact gggtgccgga aaaatcatct taattgtttc tgttttgttg tggtttatgg
     1381 caagtttcag ccccttccct actaaaacag atcatctgat aaaggaagaa attgctgcaa
     1441 agaatttcag caacccgacg gagttgcagc atttcaacga agaaaaaaaa ttagaatatt
     1501 cttatgccgg atatatggga aaaatgattg agcctgtaat aaaacctctg ggttatgact
     1561 ggaaaatagg cattgcctta attacctctt ttgctgcgcg tgaggtattt gtaggcacca
     1621 tctccacact ctattccatc ggtaaagatg ccagtgactt aacgataaaa caaaaactgg
     1681 caaaggagaa aaaagaagac ggtactgcag ttttttcatt cgccacagcg atcagtttat
     1741 taattttcta tgtatttgcc atgcaatgca tgagtaccct tgtaaccgta aaccgcgaaa
     1801 caaactcttg gaaatggcca attattcagc ttatattcat gacctcaact gcatatattt
     1861 gttcgttttt agtctatcac attttaagat agcacaatat ttcaacatca acctacaact
     1921 acaatttcac gatagtctta cttaaaattt ctccgttttg tgtgatgctg atgatatatt
     1981 cccctttggg aagctcatcc aaatgatcta taaacactga atttgctcct gctttgctat
     2041 tgccgtccca taatttttgg gttaataatt gcccggtaat attttgtata taaatggaaa
     2101 tctctccact cttttgtgca gagaaatcaa ccgtaagtga ttgattaaac ggatttggat
     2161 aaacttgcat ttggtttatc agtttgtttt cttttatggc tgaaatcact gttgaatcca
     2221 tttttacaat agaaaattta tccagcagtt ctccattggc tttgtaatat ttacatacca
     2281 aagtatgtcc gcttacttcg agtatcatac tgcctacacc tccgcttgct gcatatttct
     2341 tatacattac aggatgcatt ttattatttt cggcttctga ttttccgctg ttccccacta
     2401 ctgcataaac ggtgcctttg tttttattta cgccataggt gtatttgatg tacatacggt
     2461 tactgtcagg attgcctgat gtactgtcaa ccatcatcgt acttcttttg aatgttggcg
     2521 acaatccata atgtttgttt attaaataag agcgttcata tacatggcta tgaccattca
     2581 acactaaatc aactccatat cgctcaagga tgggcaaata ttgctcacgc atggctttca
     2641 taaaaaactc aaaccagtca tccgaatcat gtgaaccttt tgaatacgga ggctgatgcc
     2701 agtaggcaat tttaaacggc tgtgttgtat tccgcaaatc attttcgagc caggttttat
     2761 acgcatttgg caaattaaac aacaaccaag gataaagttc tgaattcata gcaacaaagt
     2821 gaatattgcc gtaatcataa gaataaaaag cttctgtatt agaagggtag ccgcccattt
     2881 ctgcattttt aaaaacctct accatatcaa aataaggtcc gtacggatta acaggattgc
     2941 ttccgtttac actgttataa tcgtggttgc ccggaatggg ataaaaaggc aaaaaacgaa
     3001 ataaagaatc atagccataa tctttgctga atacattgcg gctgtattct gcatcagttc
     3061 cgtttgtgta agtattatca cccagccaca gccaggcatc aacttttgtt gtagtcaaat
     3121 aattttgaaa ccattgcttt accaatacct gatcggtatt tccggcaccg aaatcgcctg
     3181 taacccaaaa gcggtgtatg gtagaatcgc ctataacagg ggcagtaaaa aaatgatgct
     3241 gttcatcatt tccggctaat atctgcatat tgccgtcttc aatttgataa tagtattttg
     3301 tatttgcgat taatccgctt atctgcaaaa tatgattcgt tttttgagtt gtatcagcaa
     3361 cactgttcgt caaattattg agtgctgttc catatctcac aactgccggt gtagctgcgg
     3421 ctgtacgcca aagtattttc acacttttat tggtcatgct ttgcaaataa ggcccgcgca
     3481 acaattgtgc ttgaacatgc agtaccgcaa aaaaaacaag aagagtaaat agtcgtttca
     3541 tgatttaatt ttgtttttta tttcaatttc taacgagtct ttatttttcc aaaaatcata
     3601 gagccataaa tggaaaacag gaatctggaa tttgttcagc attttttctt tatgcaattt
     3661 caaccggtag tttgtagctg catggtttgg caaatcgttt tccaagtcaa tacaaagtac
     3721 aatatattct ccttttttca aaacaaaatc caggataaaa ccgccgaaag gcatattttt
     3781 aactatggta taattgctaa aatgctgatg cagatactgc tcaatttcat ttttataagc
     3841 agcatttatg ttctgttgtt gtatcgcatt tttgtttatt accagtgctt caagcaaacc
     3901 tgttttaatt tctgtttctt gtttgtcgca ccattttaca tagctcaaaa atgcatacac
     3961 tatcgcttta cccgtattgc ctttttgttt taattctgtt tcgtactgcg taaaatattc
     4021 ctccggaaat gaggtgaaaa catgcagttg ctttttagcc cgggtaatga ttacatttaa
     4081 taactgataa cctttatccg tagatagcgg accaaaaaac tgtctgaatt ttccggatgc
     4141 atcttttccg aatgttgtgg aaataagaat aatatcccgt tcgtcacctt gtatattttc
     4201 gagatttttt acaaacaaac cattttgcaa aagtgcattc agcttatcct ggttttcttt
     4261 tgattgtgct gctttttcat acagtttatc caaaatcaaa tcccgctgaa aaatattaaa
     4321 tgtggcgatt ccgacagatt tatctgccaa catattttca tctctaaaaa caaaatcagt
     4381 tatcgcatcc gcttcacttt cattcaagcc ttcaccataa accccgttga tttgatgtag
     4441 aaataggggt tgataggcat gcgtttcagg cattggaatc aagcgattat cataaaaggc
     4501 tgcatttgaa aaccggatta aatccggatg ttcggagcgg taatgaaagc tgaggtaagt
     4561 gttgataaaa tcagatgaat ccgcatattc caaaagagaa tttgactccg ccaggaaatt
     4621 ttccacttcg ctttcttctt ctgtctgttg ttcataaaag accacctcac tggcaaaata
     4681 ggcacttggc ggcatctgat gtctgtcgcc cgaaatgatc ttttgtttcc cccg
//
LOCUS       DAOQII010000202         4703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10803_length_4703_cov_4.465361, whole genome shotgun sequence.
ACCESSION   DAOQII010000202 DAOQII010000000
VERSION     DAOQII010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10803_length_4703_cov_4.465361"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLF48_09240"
     CDS             complement(<1..262)
                     /locus_tag="PLF48_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNF49552.1"
                     /translation="MNNLSKRINNYLKQRDDNHLFRKLTLSTDLIDFSSNDYLGFSRS
                     AFIRQKVEQDLQRLSGLKFGSTGSRLLNGNTALHEELENKLAK"
     gene            complement(255..1271)
                     /gene="bioB"
                     /locus_tag="PLF48_09245"
     CDS             complement(255..1271)
                     /gene="bioB"
                     /locus_tag="PLF48_09245"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNF49553.1"
                     /translation="MNNSLKTNWTHAEIAAIYHQPLMQLMYKAAAEHSKYHQPGEVQV
                     CTLLSVKTGGCPEDCAYCPQSARYQTGVKVHKLLEIEDVVNAAKEAKANGSSRFCMGA
                     AWRELRENKDFPKVLEMVKQVNALDMEVCCTMGMLTENQAQQLKEAGLHAYNHNLDTS
                     AEEYSKIITTRDFHDRLNTIGNVRKAGISVCSGGIIGMGESHDDRIGLIFSLATLPEH
                     PESVPVNALVAVKGTPLEGMKRVAVFDMVRMIATVRLVMPKTKIRLSAGRLDMPVAEQ
                     ALCFLAGANSIFAGDKLLTTPNPDFNSDKEMFEILGLSPSVMEKAIEKDDNIVSRPVL
                     IESE"
     gene            complement(1264..1809)
                     /locus_tag="PLF48_09250"
     CDS             complement(1264..1809)
                     /locus_tag="PLF48_09250"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNF49554.1"
                     /translation="MIILQKYASRFVFFAVSFIFISACLQHAKNDNAKKILDSNKMRA
                     ILTDAFLAEGYLVEQATIQQKNTTASGGYMDSVVYPLIFNQHHVSAADFYSTFNYYQQ
                     NPDKFLPILDSVNAILDKIIPKDTTKIKPLQNDADIMPHAVDSQLSFKKLQEAQQEIF
                     LNNHPELKERIGKSRKNKKNE"
     gene            1834..2511
                     /locus_tag="PLF48_09255"
     CDS             1834..2511
                     /locus_tag="PLF48_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNF49555.1"
                     /translation="MQDITKKAKEKGVVLVAISKTKPIEDILSVYQNGHNVFGENKVQ
                     DLVSKYEQLPKDISWHMVGTLQTNKVKYIAPFIDLIHSVDSLKLAIEINKQAQKLNRT
                     INVLLEIHIGAETSKAGFSEDELKDLLDIQAFSALKNINIRGLMGMATNTKSQVLIKN
                     EFSGLADYFHEIKSHYFENQSEFDTLSMGMSGDYETALQCGSTMIRVGSAIFGEREIK
                     KNPTIPL"
     gene            2517..2999
                     /locus_tag="PLF48_09260"
     CDS             2517..2999
                     /locus_tag="PLF48_09260"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF49556.1"
                     /translation="MHKLNSAISLNRTMEKIHFVQDYKAPLKNVFSFYSDHNRWSEIM
                     PAVIRRIVDSPNPSNCNDAGSQRIIISLGGIFAETITKYIENKQIDYAITFGSPLKNH
                     KGTMKFIELSPTSCRLDYTIVFEPKIPKTGFILRNILEKQVGNGIRELARKFEKNPNF
                     "
     gene            complement(3011..3664)
                     /locus_tag="PLF48_09265"
     CDS             complement(3011..3664)
                     /locus_tag="PLF48_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNF49557.1"
                     /translation="MAKTAIIAGATGLTGGLLLDLLLKDDEYEKVIVLTRKNLYATNP
                     KISEIICDLTTLPQVENEIKGDVLFCCLGTTIKKAGTQDAFKQVDYNYPVELAKVAKK
                     NEIPEFIVISALGAKPDSLIFYNKVKGEMERDIAALNLPAFHIIQPSLIIGERKESRM
                     GEGIAQKLAPLYDKLLIGGLRKYKSIEAGQIARAMLAISKSKTKGVIRHENDELHTY"
     gene            complement(3699..>4703)
                     /locus_tag="PLF48_09270"
     CDS             complement(3699..>4703)
                     /locus_tag="PLF48_09270"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49558.1"
                     /translation="GAYWTGTKDYQSQIAMGNEFAKRGYVLASATYRQEPSFISLIFQ
                     DLMLKAVGRGVQDTKQELRYFYNSARDSANPFRIDTNRIFLCGASAGAFNVLHSVYLD
                     QQDSLPAQWLTWLNQIGGVFDTYDFKDFGSSLAGIVNINGALGNQSFLDNEHLPFLSV
                     HNIWDPEIPFNRGRPYNIASLMYVDGSNILHQKAQQLQIYNPFYIIPDNGHTSYSSDF
                     FGTVVQPFFDSTVWYMNKFFTHQLGCDLATPVFYDKIKQAKIYPNPTTDFFNIDADFS
                     DAKSIQIFDLYGKLLITLPFKTGYYTTGDLHLNNGIYILSLIDDKGEILSTGKLTITK
                     "
BASE COUNT         1471 a          869 c          812 g         1551 t
ORIGIN      
        1 atttagccag tttgttttcc agttcttcat gcaatgccgt attgccatta agcagccgtg
       61 aacctgtcga accaaatttt aatccgctta atcgttgtaa gtcttgttct actttttgcc
      121 tgataaatgc agaacggcta aatccaagat aatcatttga agaaaaatct attaaatcag
      181 tgcttaaagt taatttccta aacaaatgat tatcatctct ttgtttaaga taattattaa
      241 ttctttttga taaattattc actctcgatt aatacgggtc ggctgacaat attatcatct
      301 ttctcgatag ccttttccat aactgaaggg cttaatccaa gtatttcgaa catctcttta
      361 tctgaattaa aatcagggtt tggtgtagtt aagagtttat ctccggcaaa tatggaattt
      421 gctccggcta aaaagcagag cgcttgttct gcaactggca tatctaacct gccggcactc
      481 agtcttattt ttgtttttgg catgactaat cttacagtag ctatcattct gaccatatcg
      541 aaaacagcta ctcttttcat accttccaaa ggtgtacctt ttacagctac taatgcatta
      601 accggcacag attcaggatg ttctggcagt gttgctaaac taaaaattaa acctattcta
      661 tcatcatgac tttctcccat tccaataatt ccgccactgc aaacactgat tccggctttt
      721 cgaacattgc caatcgtatt taatcggtca tgaaaatctc gggtagtgat aattttgctg
      781 tattcttctg cagaagtatc taaattgtgg ttataggcat gaagtccggc ctctttaagc
      841 tgttgagcct gattttctgt cagcatgccc attgtacagc atacttccat atcaagagca
      901 ttaacttgct tgaccatttc caatactttg ggaaaatctt tattttctct aagttctcgc
      961 caggccgctc ccatacaaaa acgactggaa ccatttgctt tggcttcttt agcagcattc
     1021 acaacatctt caatttcaag aagtttatga actttaaccc ctgtttgata tctggcagac
     1081 tgtggacaat aagcacaatc ttccggacaa ccacctgttt tgacagaaag cagggtacat
     1141 acttgcactt caccaggttg gtggtatttg gaatgttcgg cagcagcttt atacatcaac
     1201 tgcatcaaag gctgatgata tatggcggca atttctgcat gtgtccaatt ggttttcaag
     1261 ctattattca ttttttttat tttttctgct ttttcctatt ctttctttta attcaggatg
     1321 attattgaga aaaatttcct gttgtgcttc ttgtaacttt ttgaaactga gctggctatc
     1381 cactgcatgc ggcatgatat ctgcatcgtt ttgaagtggc tttatttttg tagtgtcttt
     1441 tggaataatt ttatccaaaa ttgcattaac actatccaaa ataggcagga atttatcggg
     1501 gttttgctgg taataattaa acgtagaata aaaatcagca gcagagacgt ggtgttggtt
     1561 gaaaattaat ggataaacga cggaatccat atatcctccg gaagctgttg tatttttttg
     1621 ctgaattgtc gcttgttcta ctaaataacc ttcagctaaa aatgcatctg tcagaatagc
     1681 tctcatctta ttactgtcta atattttttt tgcattgtca ttttttgcat gctgaaggca
     1741 tgcagaaata aatataaagg aaacagcgaa gaatacaaac cgagatgcat atttttgtaa
     1801 aataatcacg ttgctatggt caatttaagt gctttgcaag atataacaaa aaaggctaaa
     1861 gaaaaaggtg tagttttagt ggctatttcc aaaaccaaac ccattgaaga tattttatct
     1921 gtctatcaaa atgggcataa tgttttcggc gaaaataaag tacaggattt agtgagcaaa
     1981 tatgaacaat tacccaaaga catcagttgg cacatggtgg gtacactgca aaccaataaa
     2041 gtgaaatata ttgctccttt tatagattta atccattcgg ttgacagtct gaaattagct
     2101 attgaaatca acaagcaagc acagaaacta aaccggacaa ttaatgtttt actggaaatt
     2161 catatcggtg cggaaacatc caaagccgga ttttcggaag atgaattgaa agatttactg
     2221 gatatacaag ctttttcagc attaaaaaac atcaatatta gagggttgat gggtatggca
     2281 acgaatacta aaagtcaagt gctgataaag aatgaatttt caggattggc agattatttt
     2341 catgaaataa aatcacatta ttttgaaaat caatctgaat ttgatacctt atctatggga
     2401 atgagtggtg attatgaaac ggcattgcaa tgcggatcaa caatgataag agtgggttca
     2461 gctatttttg gagaacggga aatcaaaaaa aatccgacta ttccactata aaatgtatgc
     2521 ataaactaaa ttcagctatt tcattaaatc gtactatgga aaaaattcat tttgttcaag
     2581 attataaagc accgctaaaa aacgtttttt cattttacag tgatcacaat cgctggagtg
     2641 aaattatgcc ggccgttatt cgccgtatag tggacagtcc aaatccatct aattgtaatg
     2701 atgcaggctc acaacgaatt ataatttctt taggtggtat ttttgcagaa acaattacga
     2761 agtacattga aaacaaacag attgattatg caatcacatt cggtagtccg ctaaaaaacc
     2821 acaaaggcac gatgaaattt attgagcttt caccaacaag ctgtcgcttg gattatacga
     2881 ttgtatttga acctaaaatt ccgaaaacag gatttattct gcgtaacatt ttagaaaaac
     2941 aagtaggtaa cggcatccgc gaacttgcaa gaaagtttga aaaaaatcct aacttttaaa
     3001 ggatatcttt tcaataggta tgcagttcat cattttcatg tcgaatcacg cctttcgttt
     3061 ttgatttaga aatagcgagc atagctcttg cgatttgtcc ggcctcaatc gatttgtatt
     3121 ttcgcagacc gcctatcagc agtttgtcat aaagaggagc cagcttttgg gcaatcccct
     3181 ctcccattct cgattcttta cgttcgccaa tgatcaaact gggttgtata atgtgaaagg
     3241 caggtagatt taaggcagca atatcccgtt ccatttcacc ttttactttg ttataaaaaa
     3301 taagtgaatc gggtttagcg cctaatgccg aaatgacaat aaattcaggt atttcgttct
     3361 tttttgcaac ttttgccaat tcaacaggat aattgtaatc tacttgttta aatgcatctt
     3421 gtgtacctgc tttttttatc gtagtcccca ggcagcaaaa taatacatca ccttttattt
     3481 cattttctac ttgaggcaac gtagttaaat cgcatataat ttctgatatt tttggatttg
     3541 tagcatacag atttttgcga gtcaatacta ttaccttttc atattcatca tcttttagca
     3601 ataaatccaa taataatccg ccggtcaggc ctgtagctcc tgctatgatt gctgttttag
     3661 ccatttttaa tctcgtttta gtttaaaatt atgcaaagtt atttagtaat tgtcaactta
     3721 ccggtagata aaatttcacc tttatcgtcg attaacgata atatataaat tccattgttt
     3781 agatgcaaat ctccggtagt ataatatcct gttttgaaag gcaaagtaat taaaagttta
     3841 ccatataaat caaaaatctg aattgattta gcatcactga aatctgcatc gatattaaaa
     3901 aaatcagtag ttggattcgg atatatttta gcttgtttta ttttgtcata aaaaacagga
     3961 gttgccaaat cacatcctaa ttgatgcgtg aaaaatttat tcatatacca taccgtactg
     4021 tcaaaaaaag gctgaacgac cgtgccgaaa aaatcggaag aatatgaagt atgaccatta
     4081 tccggaataa tgtaaaacgg attataaatt tgcagttgct gtgctttctg atgcaagata
     4141 ttacttccat ctacatacat caaagaagca atgttataag gtctacctcg gttaaatggt
     4201 atctccggat cccaaatatt gtgtacactt aaaaagggca aatgttcatt atccaagaat
     4261 gattgattac ccagtgcccc gttaatattg acaatacccg ctaaacttga tccaaaatct
     4321 ttaaaatcat aagtatcaaa tactcctcct atttgattaa gccaggtaag ccattgagcg
     4381 ggcaggctgt cttgctgatc aagatatacg gaatggagta cattaaaagc tccggcactt
     4441 gcaccacata aaaatatacg gttggtatca attctaaagg gattggcact atctcttgcc
     4501 gaattataaa aatagcgcaa ttcctgcttg gtatcttgta cccctctgcc tacggctttc
     4561 agcattaaat cctgaaaaat caaagatata aaactcggct cctgacgata agttgcagat
     4621 gccagtacgt atcccctttt ggcaaactcg tttcccattg ctatttgact ttgatagtct
     4681 tttgtgcccg tccaataagc acc
//
LOCUS       DAOQII010000203         4660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_10946_length_4660_cov_4.408686, whole genome shotgun sequence.
ACCESSION   DAOQII010000203 DAOQII010000000
VERSION     DAOQII010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10946_length_4660_cov_4.408686"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLF48_09275"
     CDS             <1..196
                     /locus_tag="PLF48_09275"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNF49559.1"
                     /translation="INPTAGIFELIPDNIPLIGNLDEAAAVFVLISALKYFGFELPNI
                     FKKEVKQDSTNKPTIIIDKK"
     gene            complement(262..801)
                     /locus_tag="PLF48_09280"
     CDS             complement(262..801)
                     /locus_tag="PLF48_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNF49560.1"
                     /translation="MQAHYKYCSNCGNEVVIKFVEGRDRIVCMHCQTIHYTNPKIIVG
                     ALCYWEDKVLLCKRAIEPRQGFWNIPAGFLEDNEQAEAGALREVWEEAGAAAKIIQPY
                     VVYNLPQANQVYIHFLAGLIDGIVRNGEESIESKLMTEEEIPWKEMAFTSSTFALRHF
                     FNERKSGKFGIHLGTFPQK"
     gene            868..1317
                     /locus_tag="PLF48_09285"
     CDS             868..1317
                     /locus_tag="PLF48_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF49561.1"
                     /translation="MLTVNCQILTFGSDLQKESIDLRYDVLRKPLGLHYTPEQLAAEA
                     NQMHFAAIYDNKVIGILLIEKLNDTACKMRQVAVATEFQNKGIGKKLVEFAELELENR
                     RINSIILHARDTAIPFYLSLNYSIISDTFYEVGIPHKKMSKKIQLFS"
     gene            1414..1971
                     /locus_tag="PLF48_09290"
     CDS             1414..1971
                     /locus_tag="PLF48_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49562.1"
                     /translation="MKPKFFALAVAVLFISKIFAKDEAYYPALMYNYFPKNLAEKNWH
                     AVVYGKVIKVENFGKTDVEEASQKLTIKMDTILMDKGFDDEQLNDYSYLIMHGFQNMQ
                     TGDKVLLFLVEYDEDYSMPNQYYLKVQNKQDITYQTMLSYISNYYKTKQVHNAYLMNN
                     TLLTTLLSTPFNYENQYYSYNSTKK"
     gene            2065..2640
                     /locus_tag="PLF48_09295"
     CDS             2065..2640
                     /locus_tag="PLF48_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNF49563.1"
                     /translation="MSVFSIIKQRRSIDPDDFNGQLVDKHIIDEMLESANWAPTHGLT
                     EPWRFVVITQEFLSDFGLFHADLFKQKTAPEHFLEKKYNKIKNRSALCSHVIICVNKR
                     GNKTNIPELEELAATSCAIENMLLVATAHHIGTFWSTGGMCYDADFKKHFGFDEQDQV
                     MGILYIGHYDIPQPTGIRLTDWQDKVRYYPA"
     gene            2717..3817
                     /gene="ychF"
                     /locus_tag="PLF48_09300"
     CDS             2717..3817
                     /gene="ychF"
                     /locus_tag="PLF48_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNF49564.1"
                     /translation="MGLQCGIVGLPNVGKSTLFNAVSNNAKAQASNYRFCTIEPNVGL
                     VNVPDPRLNKLAELVNPNRIVPTTIEFVDIAGLVKGASKGEGLGNKFLANIREVDAII
                     HVIRCFEDDNILREEGAINPINDKDIIDTELQLKDLESVEKKIAKIEKQARVGGDAKA
                     KHELDVLLRCKACLDNGKNVRSLNLPKEEKPFIADICLLTDKPVLYVANVDEASILTG
                     NAYSKALEAVGKEENAEVIVLNNSVEAQLSEMAEDDRTLFQSEFGLTEPGLNKLIRSA
                     YKLLNLITYFTAGVQEVRAWTIHEGWKAPQAASVIHTDFEKGFIKAEVIAYEDFIHYG
                     SESACRDHGKFKIEGKEYTVKDGDVMHFRFNV"
     gene            complement(3895..>4660)
                     /locus_tag="PLF48_09305"
     CDS             complement(3895..>4660)
                     /locus_tag="PLF48_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49565.1"
                     /translation="GHKAQTPLFNQMSAHYIELAPDSLTMLGLGDVLYRAPETTAGGE
                     NAIDFSKSVLAGDNETFFSIPIKDVTPGTYKWLRVSLAYQNYDIKVQYRTAAYNGGAP
                     ILADANVASFVGFNTYITSLNVKGENLTLNANKKQGYGACKVFNLPFGYNYPLFDFQS
                     PAGATTVPNPIFFTSPVPPGSCVVTGRFDAAGFKITGNETSDVIVTVSISTNNSFEWK
                     DKNGDGRYEPVDGLNGNAPMDSVVDMGVRGLIPIVN"
BASE COUNT         1530 a          846 c          866 g         1418 t
ORIGIN      
        1 gataaacccg actgcgggaa ttttcgaact catcccggat aatattccgc ttattggaaa
       61 tctggatgaa gctgcagctg tattcgtgtt aatttctgcc ctgaaatatt tcggctttga
      121 attgccaaat attttcaaaa aagaagtaaa acaagacagc acaaacaaac cgactatcat
      181 tattgataag aaatagtgtt tgtaatttgc ctgttataaa ttttggtttg cgccacctta
      241 cggtggcgca tttttatttg cctacttttg agggaatgta cccaggtgaa taccaaattt
      301 ccccgatttt cgttcattaa agaagtggcg caaggcaaat gtagatgacg taaatgccat
      361 ttctttccag ggtatttctt cttctgtcat taatttactt tctatacttt cttcaccatt
      421 gcgaacaatt ccatctatta gaccagctaa aaaatgaata tatacttgat tggcttgcgg
      481 caaattataa actacataag gctgaataat tttagctgct gcaccggctt cttcccatac
      541 ttcgcgcagt gcaccggctt ctgcttgttc attatcttct aaaaaacctg ccggtatatt
      601 ccaaaagccc tggcgcggct caatggcacg cttacacaac aacactttat cttcccaata
      661 gcaaagtgct ccaacaatta ttttaggatt tgtatagtga atggtttggc aatgcatgca
      721 aacaattcta tcgcggcctt ctacgaattt tatgactact tcatttccac aattactgca
      781 atatttataa tgagcttgca tacgataaag ataattatat tttggtgctg tgctaatttc
      841 aattattttt gaatgcaatt ctcaagtatg cttacagtaa attgtcaaat attaacattc
      901 ggctctgacc tacaaaagga atccatagac ttgaggtacg atgtattacg caaaccttta
      961 gggttacact atactcccga acaactggca gcagaagcca atcaaatgca ttttgctgca
     1021 atttatgata ataaggttat cggcatttta cttattgaaa agttaaatga tactgcttgc
     1081 aagatgcgcc aggtagccgt agccactgaa ttccaaaata aaggaattgg taaaaaactg
     1141 gtggaatttg cagaactgga attggaaaat aggagaataa attcaatcat cctgcatgcc
     1201 agagatactg caatcccctt ttatttgtcc ttaaattaca gcatcatctc tgatacattt
     1261 tatgaagtgg gcatccctca caaaaaaatg tcaaaaaaaa ttcaactttt ctcttgacaa
     1321 ttcgataaag tttagctaac tttaaattgt aatttaaata tcacgagata ctaagttaca
     1381 aaacccttgt agaaacataa taaaataacc ctcatgaaac ccaaattctt tgcattggca
     1441 gttgccgtgc tattcatttc taaaatcttt gcaaaagatg aagcttacta tccggctttg
     1501 atgtacaatt atttcccaaa aaatctggct gaaaaaaact ggcatgcagt tgtttatggc
     1561 aaagttatta aagttgaaaa tttcggaaaa acagatgtag aagaagcctc acaaaaactg
     1621 actattaaaa tggatacaat tttaatggat aaaggttttg atgatgagca gcttaatgat
     1681 tacagttact taattatgca tggctttcaa aatatgcaaa ccggagataa agtactgctt
     1741 tttctggtgg aatatgatga agattattct atgccgaatc aatattacct gaaagtgcaa
     1801 aataaacaag atatcactta tcagactatg ctatcgtata tcagcaatta ttacaaaaca
     1861 aaacaagtac ataatgccta tttgatgaac aatacattac tcactacttt attatctact
     1921 ccgtttaatt atgaaaacca gtattactcc tataattcta ccaaaaaata aactttcata
     1981 gctatttgtt ttctgccggc gtatttctta cgtcggctat ttttttgttc agtctaaatt
     2041 gatgtatttt tgtaaaaaag aagtatgtct gtattctcca ttatcaaaca acgcagaagc
     2101 atcgatccgg atgattttaa tggtcagctg gttgataaac acatcataga tgaaatgctg
     2161 gaaagtgcca attgggcacc cacacacggt ttgacagaac cctggaggtt tgtagttatc
     2221 actcaggaat ttttgtctga ttttggtctt tttcatgctg atttatttaa acaaaaaaca
     2281 gcgcccgaac attttttgga gaaaaaatat aacaaaataa aaaacagaag tgccttatgt
     2341 tcacatgtga ttatttgtgt caacaaaaga ggcaataaaa caaatatacc tgaacttgaa
     2401 gagctggcag ctacgagttg tgcgattgaa aacatgttgt tagtagctac tgctcatcat
     2461 atcggcacat tctggagcac aggcggtatg tgctatgatg ctgatttcaa gaaacatttc
     2521 ggatttgatg aacaggacca agtgatgggg attttataca ttggtcatta tgatattcca
     2581 caacctaccg gcatacgctt aactgactgg caagacaaag tgcgatatta tcctgcttaa
     2641 taactggaaa aatgtatctt tgcacccgaa ttgcaatttt gctttcatat taatatttaa
     2701 aataaataag taaaacatgg gtttacaatg tggtattgta ggtttgccaa acgtaggaaa
     2761 atcgacactg tttaatgccg tttctaataa tgccaaagca caggctagta attatcgctt
     2821 ttgtacaata gagccaaatg taggcttggt aaatgtacca gatccccgac taaataagtt
     2881 ggcagaactg gtgaatccca atcgtatcgt gcctacaaca attgagtttg tagatattgc
     2941 cggtttggta aaaggagcca gcaaaggtga aggcttgggc aacaaatttt tagccaacat
     3001 acgagaagtg gatgccatta ttcacgtaat ccgctgtttt gaagatgata atatcttgcg
     3061 tgaagaagga gctatcaatc ccataaacga caaagatatc attgatacag aattgcagtt
     3121 gaaggatttg gaaagcgtgg aaaaaaagat agccaaaatt gaaaaacaag cccgggtagg
     3181 cggcgatgcg aaagcaaaac atgaacttga tgtattattg cggtgcaaag cttgcttaga
     3241 taatggaaaa aatgtgcgtt ctttaaattt accaaaagaa gaaaaaccat ttattgcaga
     3301 tatttgttta ctgacagata agcctgtatt atatgttgcc aatgtggatg aggcctctat
     3361 cttaactgga aatgcatact ccaaggcatt ggaagctgta ggcaaagaag aaaatgcaga
     3421 agtcattgtt ttaaataaca gtgtggaggc ccagctttct gaaatggctg aagatgacag
     3481 aacattgttc caatcggagt ttggattgac agagcctggg ttaaacaaat tgattcgctc
     3541 ggcttacaag ttgttaaatc tgattaccta ttttacagcc ggagtacaag aggtgcgagc
     3601 atggactata cacgagggat ggaaagcccc acaggcagcc agtgttatcc atactgattt
     3661 tgagaaagga tttattaaag ctgaagttat tgcatacgaa gattttattc attacggctc
     3721 agaaagtgct tgtcgcgatc atggcaaatt taagatagaa ggcaaagaat ataccgtaaa
     3781 agacggcgat gtcatgcact ttaggtttaa tgtataatcc ggttaatgat gaatgctgaa
     3841 tgatgaatgg ttattttatc atcatttgct aactaaacaa ctgccaactg cttactaatt
     3901 cactatcgga attaatccgc gtacacccat atccactaca gagtccatgg gggcatttcc
     3961 atttaatcca tctacaggtt catatctgcc gtcgccattt ttatctttcc attcaaaact
     4021 attattggta gagattgaaa ctgtaacaat cacatctgat gtttcgttcc ctgtaatttt
     4081 aaatcctgct gcgtcaaatc tgcctgtaac tacgcaagaa cccgggggca caggcgatgt
     4141 gaagaaaata ggatttggaa cggttgtcgc gcctgccgga gattgaaaat cgaataaagg
     4201 atagttataa ccaaatggaa gattaaatac tttgcatgca ccatagcctt gttttttgtt
     4261 tgcatttaaa gttaaattct ctccttttac attcaatgat gtaatataag tattgaaacc
     4321 aacgaatgaa gccacgttgg catctgccaa aataggcgca ccaccattat aagctgcagt
     4381 acgatattgt actttaatat catagttttg ataagccaga gaaacacgta accacttgta
     4441 tgtgcccgga gttacatctt ttatcgggat agaaaaaaat gtttcgttat cacctgctaa
     4501 tacagattta ctaaaatcaa tggcattttc gccacctgct gtcgtttcag gtgctctata
     4561 caaaacatct cccaaaccta acatcgttaa tgaatcagga gccagttcga tataatgtgc
     4621 actcatttga ttaaataaag gagtctgtgc tttatgacca
//
LOCUS       DAOQII010000204         4631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11036_length_4631_cov_4.707168, whole genome shotgun sequence.
ACCESSION   DAOQII010000204 DAOQII010000000
VERSION     DAOQII010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11036_length_4631_cov_4.707168"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1883
                     /locus_tag="PLF48_09310"
     CDS             375..1883
                     /locus_tag="PLF48_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF49566.1"
                     /translation="MTLKRINYLLFLTILAGTLAVGCKKGSSGGSSSTTGWNYNDKKW
                     GGFEKSNNTNQITGPNLVLIEGGTFAMGATEQDLLYDNNNVQRRISVSSFYMDETEVT
                     NIDYNEYVYWLERVFGTDHPQYVKAAKPDSMCWRRNLAYNEPYVKYYFTHPAYNTYPV
                     VGVNWLQANEYCKWRTDRVNEMILVKEGYLDLNTNQKNEDNFNTEAYAYGMYQGATKK
                     QKEDLNPNGAGQRNVNFKDGILLPDYRLPTEAEWEYAALGLRGNMPLKGEEVVTDRRI
                     YPWDGATFRYQKHNKSQGDFMANFMRGRGDYMGVAGALNDNAEITSDVHANMPNDFGL
                     FNMAGNVNEWTMDVYRPMTESDAQDLNSFRGNVFTTMSTEEGLDSMGRIKYVRENDED
                     LTNRRNYRKSYAIDYNDGDSSSEVEYQYGISTLINNKARIYKGGSWRDRGYFLSPGTR
                     RFLNEDQSTDDIGFRCAMVRVGSPDGNFFGGKAFSETNKVVNKNKKSRKYSK"
     gene            1941..3227
                     /gene="murF"
                     /locus_tag="PLF48_09315"
     CDS             1941..3227
                     /gene="murF"
                     /locus_tag="PLF48_09315"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNF49567.1"
                     /translation="MQISAIYQLFLKSNGVTTDTRNIQDNQLYFALKGSSFNGNTFAM
                     QALECGACYAIIDEAEYAFNERCILVENVLETLQQLGLYHKIQIQPKHTIAITGSNGK
                     TTTKELVNAVLATTYKTHYTKGNLNNHIGIPLTLLQMPAGTEIAIIEMGANHQHEIEA
                     YCTYVEPDFGIITNCGKAHLEGFGGVEGIRKGKGELYDYLKSNNGHVFVNGDDKQLLS
                     MLEERSITAFTTYGSSEINNYYATILNENPYLKIRFENTEIDANLFGSYNFTNMMCAI
                     AVGKYFGVANPEIKQGIESYTPQNARSQVVEFNGAEIILDAYNANPSSMQHALESFSK
                     PDVKDKWVILGDMFELGEYAANEHQFIADLCKQLHFEKIVLVGNEFFRTTVTSSEMII
                     FPTTIEAKNWFLQQPLSGKTILLKGSRGMKMESLLA"
     gene            complement(3234..3839)
                     /locus_tag="PLF48_09320"
     CDS             complement(3234..3839)
                     /locus_tag="PLF48_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49568.1"
                     /translation="MLTTSLKIHVADTLGQNVIGAKVYLHKNKDSWASWRNPIDSLYT
                     NAYGDALFNNLSDTVRYYFNITSGESTNRFTKYKLTNKLLAFEENKLTVAIRPYTEWE
                     KIMGGNAHLKWKLLKLKNINGVPFFDYPVITDMYMDGRWYDSNGRLGLWWFSADNKKI
                     YYDYAATGAVVESEMINLTPALFEAKINFFGIIMLIDMVPV"
     gene            complement(3932..>4631)
                     /gene="prfB"
                     /locus_tag="PLF48_09325"
     CDS             complement(3932..>4631)
                     /gene="prfB"
                     /locus_tag="PLF48_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131330388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNF49569.1"
                     /translation="NAGAGGTEACDWGSMLLRMYTMWANKNGYKVTELDYQSGDVAGI
                     KSVAIEIDGDYAFGMLKGENGVHRLVRISPFNAQGKRMTSFCSVFVHPVVDDSIEINI
                     NPADLEWDVFRAQGAGGQHVNKTESAVRVRHLPSGIVCECQTQRSQIQNRETALKMLK
                     SKLYEEELRKKLEARDAIEAQKMKNEWGSQIRSYVLDDRWVKDLRTGFKSTNPDNVLD
                     GDLNGFFKAYLLHH"
BASE COUNT         1537 a          833 c          859 g         1402 t
ORIGIN      
        1 tggaagaaaa ttccacgcac tgtttgtatt acagtttgta aaggttaaga gcttagtatt
       61 taaattccag tttaattgaa tattttgtga tttcctgaaa gcatcttgtg caaatacttg
      121 aatagtaaaa gtaaaacaga ttaaaaatag cgtaaatatt ttgcgcaaaa aactcatgcc
      181 gtcaattgaa attcagtatt aattagattt aaaatgtaga attcagatca tttttcgcat
      241 ttatcttttg caagtttaca acaattcgca atattaagta ataaattccg ttaaataagt
      301 tgcacgaaat gtttggtaaa atgaaaataa aactttattt ttggtcttta tatctaaata
      361 taaagccaac aacaatgact ttaaagagaa ttaattactt attgttcctg acaatactcg
      421 caggaacttt agccgtagga tgtaagaaag gcagtagtgg cggttcttca tcaacaaccg
      481 gctggaatta caatgacaaa aaatggggcg gttttgaaaa atcaaacaac accaatcaga
      541 ttacaggacc aaacttggtg cttattgaag gtggtacgtt tgctatgggc gctactgagc
      601 aagatttgct ttacgacaac aacaatgtac aacgtagaat tagtgtatct tctttttata
      661 tggatgaaac ggaagttacc aatatcgact ataatgaata tgtttattgg ttggagcgcg
      721 tattcggtac cgaccacccg caatatgtga aagctgcaaa gcccgactct atgtgctgga
      781 gaagaaatct ggcatacaat gagccatatg taaaatatta ctttacacac cctgcataca
      841 atacttaccc tgtagtaggt gtaaattggt tgcaagccaa tgaatactgc aaatggcgta
      901 cagaccgtgt aaatgaaatg attttagtaa aagaaggcta cttagatttg aatactaacc
      961 aaaaaaatga agataacttc aatactgaag cttatgcata cggcatgtat caaggtgcaa
     1021 ctaaaaaaca aaaagaagac ttaaacccga atggtgccgg tcaacggaac gtaaacttca
     1081 aagatggtat cttattaccg gattaccgac ttccaacaga agctgaatgg gaatatgctg
     1141 ctttaggctt gagaggcaat atgccattga aaggagaaga agttgttact gacagaagaa
     1201 tctatccttg ggatggtgct acattccgct atcaaaaaca caacaaatca caaggtgact
     1261 tcatggctaa ctttatgaga ggcagaggtg actatatggg tgttgctggt gcattaaatg
     1321 acaatgcaga aatcacctct gatgtacatg ccaatatgcc aaatgacttt ggtttattta
     1381 atatggcagg taatgtaaat gagtggacta tggacgttta tcgcccgatg acagaatctg
     1441 atgcccaaga cctgaacagc ttccgtggta atgtatttac tactatgtca actgaagaag
     1501 gattagacag tatgggtcgt attaaatacg ttcgtgaaaa tgacgaagat ttgacgaaca
     1561 gaagaaatta ccgcaaatct tatgcaatag attataatga cggtgattca tcttctgaag
     1621 ttgaatatca atatggtatt tctactttga taaacaacaa agcaagaatt tacaaaggtg
     1681 gttcttggag agacagaggt tatttccttt ctccgggtac tcgtcgtttc ttaaatgaag
     1741 accaaagtac tgatgatatt ggtttccgtt gtgcaatggt tcgtgtaggt tcacctgatg
     1801 gaaatttctt tggcggaaaa gctttcagtg aaacgaataa agtagtaaac aaaaacaaaa
     1861 aatcaagaaa atacagtaaa taagataata agctaaataa aaaagtcctg attcgttcag
     1921 gactttttta tttttacacc atgcaaattt cagcaatcta ccaactcttt ttaaaatcaa
     1981 acggagtaac tacggatacc cgtaatatac aagacaatca actttatttt gctttaaaag
     2041 gcagttcttt caatggcaat acttttgcta tgcaagccct ggaatgcggt gcatgctatg
     2101 caattattga tgaagctgaa tatgcattca atgaacgttg tattttggta gagaatgtac
     2161 tggaaacatt gcaacagctc ggattgtatc ataaaattca aattcagccc aagcatacaa
     2221 tagccattac cggatcaaat ggcaaaacaa cgactaaaga attagtgaat gctgttttag
     2281 cgactacata taaaacacat tatactaaag gtaatctgaa taaccatatt ggaattccgc
     2341 tcacgttatt gcaaatgcct gccggcactg agatagccat catagagatg ggtgccaatc
     2401 atcagcatga aatagaagct tattgtactt acgtagaacc tgattttggg ataataacaa
     2461 actgcggaaa agcacatttg gaaggatttg gtggggtaga ggggataaga aaaggaaaag
     2521 gcgaattgta tgattatcta aaatcgaata acggacatgt ttttgtcaat ggagatgaca
     2581 agcagttgtt gtctatgttg gaggaacgca gtattacagc ttttacgaca tatggcagtt
     2641 ctgaaataaa taattattac gccactattt taaatgaaaa tccctatttg aaaatacggt
     2701 ttgagaacac ggaaatagat gcaaatttat ttggttctta taatttcacc aatatgatgt
     2761 gtgccatagc tgtcggcaaa tattttggag ttgccaaccc tgaaataaaa cagggcatag
     2821 aaagctacac accacaaaat gcacgttcgc aagtcgtaga atttaatggt gctgaaatta
     2881 ttttggatgc gtataatgcc aatccttcca gtatgcaaca tgcattagaa agtttttcaa
     2941 aaccggacgt aaaagataaa tgggtgattt taggcgatat gtttgaactg ggtgaatatg
     3001 cagctaatga acatcaattt atcgcagacc tttgcaagca attacatttc gagaaaattg
     3061 ttttagtagg aaatgagttt ttcagaacaa ctgtcacatc atccgaaatg attatttttc
     3121 ctacaacaat tgaagccaaa aactggtttt tgcaacaacc gctttcaggc aaaacaatac
     3181 tgttaaaggg ttccagagga atgaaaatgg aaagtttatt agcataaact attttatacc
     3241 ggtaccatgt caattaacat gataatgccg aaaaaattta tcttagcttc aaacaaagcc
     3301 ggagtcagat ttatcatttc actttcaact actgcgcctg ttgcagcata gtcataataa
     3361 atctttttat tatctgcact aaaccaccac aagcctaacc tgccgttgct gtcgtaccat
     3421 ctgccgtcca tgtacatatc tgtaataaca ggatagtcga aaaatggaac tccgtttata
     3481 ttctttaatt ttaataattt ccatttcaga tgagcatttc cacccattat cttttcccat
     3541 tcagtgtaag gacgaatggc tactgttaac ttgttttctt caaatgcaag taacttatta
     3601 gttaatttat acttagtaaa acgattagta ctttctcctg aagtgatatt aaaatagtag
     3661 cgcacagtat ctgataaatt attgaaaagt gcatcaccgt atgcattcgt gtataagcta
     3721 tctatcggat tcctccatga tgcccagcta tctttatttt tgtgtaaata cacttttgca
     3781 cctatcacgt tttgccctag tgtgtctgca acatgaattt ttagtgatgt agttaacaac
     3841 tcagcctttt tacaagctat tatgcttata caataacaga caattactgt aaattttatt
     3901 ccggataaaa atcgcatttt agtttcttta cttaatgatg taacaaataa gccttaaaaa
     3961 atccgtttaa atcgccatcc aatacattat ccggattggt ggacttaaat cctgtacgca
     4021 aatcttttac ccaacggtca tccagcacat aactgcgaat ttgtgaaccc cattcattct
     4081 tcattttttg tgcttctata gcatcgcgtg cttccagttt cttgcgcaat tcttcttcat
     4141 ataattttga tttcagcatt tttaaggctg tttccctgtt ttgtatttga gaacgttgcg
     4201 tttggcattc gcaaacaatt cccgaaggaa gatggcgcac acgtacagca gattctgtct
     4261 tgtttacgtg ctgtccaccg gcaccttgtg ctctgaaaac gtcccattcc aaatcggcag
     4321 gattgatatt gatttcaatc gaatcatcga ctacgggatg aacaaacacc gaacaaaaag
     4381 atgtcatgcg tttgccttgt gcattaaacg gagagatgcg caccaaacgg tgtacaccgt
     4441 tttcaccttt tagcatacca aaagcataat cgccgtcaat ttcgatagca acacttttta
     4501 taccggcaac atcacccgat tgataatcca gttcagttac tttatagccg tttttgtttg
     4561 cccacatggt gtacatccgt aagagcatac taccccagtc acaagcttca gtaccgccgg
     4621 ctccggcatt t
//
LOCUS       DAOQII010000205         4620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11074_length_4620_cov_4.673384, whole genome shotgun sequence.
ACCESSION   DAOQII010000205 DAOQII010000000
VERSION     DAOQII010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11074_length_4620_cov_4.673384"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLF48_09330"
     CDS             complement(<1..386)
                     /locus_tag="PLF48_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNF49570.1"
                     /translation="MQDTFRHQGLRKQLVKELREQGIQDEAVLSVIEKIPRHFFIDDS
                     FEFKAYENIPFPIGKGQTISNPFTVAYQTELLAIEPGDKVLEIGTGSGYQAAVLAEMD
                     ALVYTIERHESLKLKTEKLLQDLGYN"
     gene            564..1556
                     /locus_tag="PLF48_09335"
     CDS             564..1556
                     /locus_tag="PLF48_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF49571.1"
                     /translation="MKNSFICLLVFVYSILTVKSNTISGSILDELQKPLEFCNVLLHQ
                     YNDSIFLEGTTSDSTGSFDFKNIKPGNYFIEIIYIGYKTDTLNNIQVTQDKDVDLAPV
                     TMQPDATVLGNVEIKSTRPVIERKADRLVYNVENSTKSSGENSFDLLRSVPGVSITGN
                     DQINVNGKSGVQVMINGRVENLSGDQLVTFLKSIQSSNIKKIEVISNPSAKFDAEAKG
                     GIINIQLKNNSRSGVNGSVYVNYRQNKYASTEAGFNLNVNYKKWVVSMAYNNSYGKGY
                     QDHIFTRDFTFNDSIQQYDEKGKDVNRFMGHFGNLQINYNINDKHTIGITSETF"
     gene            1632..2990
                     /locus_tag="PLF48_09340"
     CDS             1632..2990
                     /locus_tag="PLF48_09340"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNF49572.1"
                     /translation="MQKTNNTTKTSGVSPTVNFHYIGNLDTSGIKLEFNYDFAFFRFF
                     TNSSLMTDDYDKQENYISNLYNFTQNNPFIVHLHTSNLNYYKPLKKGHNIETGFKFTW
                     TKTGNDVQFKNWDGTQYILDAGKSNKFEYIENINALFGIWSKEWKKNWSTNLGLRIEQ
                     TNTNQYSETVNSRTKKHYVDFFPSLFIQKTIKEKHSLNINFSRKIQRPNFEDLNPFEY
                     YLSPYSIWTGNANLKPQLVNLTEITYTFKNTYSFFINHENLNNNSTHLVFQDDSTKIS
                     TYRASNFRVRNNLNIGLSINKDLFKWWSISANATYTFFRYNSIVNDEEFKVSSHKGNF
                     SVDNTFRLPKDFQINVFAFYTTPHLDATDIMKSDGMVNISISKSFLKKALKIKLSGND
                     IFRTKNFSYTTDFFNVHSVSINKFSSAFVGLTVSYNFNKGKQFKNTVISKSNEDEKSR
                     IH"
     gene            3181..4272
                     /locus_tag="PLF48_09345"
     CDS             3181..4272
                     /locus_tag="PLF48_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HNF49573.1"
                     /translation="MSNLNILPLKDWGFNFERPIIISGPCSCETEEQMLETAKRLADK
                     GIDVLRAGIWKPRTRPGMFEGIGKDGLTWLIEAGKQIGKPTTVEVATGQHVEDALKAG
                     VDILWIGARTTVNPFSVQEVADALKGVDIPVLIKNPINPDLQLWLGSMERINAVGIKR
                     IGAIHRGFSSYEKSKYRNKPMWEIPVEMKRLYPHMPIIVDPSHICGARELIPSVAQKA
                     LDLDFDGLMIESHRDPDQAWSDAQQQVTPERLGEIISGLIVRHSKLDDYEENNLEALR
                     AQIDRIDNYMLEIMGERMDIARKIGEFKRDNGITILQTNRWDEIINDRIKKASKKELT
                     EDFVREMMDAIHQESIRHQTKIMNNILAE"
BASE COUNT         1642 a          799 c          836 g         1343 t
ORIGIN      
        1 ttattgtaac ccaaatcctg aagcaatttt tcagttttca gtttcaagga ttcatgacgt
       61 tctatcgtgt aaaccaatgc atccatttct gccagtactg ctgcctgata gcccgaaccg
      121 gtaccaattt ccagcacttt atctcccggc tctatggcta acagttcagt ttgataagca
      181 acagtaaacg ggttggaaat ggtttgtcct tttccaatag gaaagggtat attctcgtaa
      241 gctttaaact caaaagagtc atcaataaaa aagtgccggg gtattttttc tataacactc
      301 aaaacagcct catcttgtat tccttgctca cgcaattctt ttaccaattg cttgcgcaaa
      361 ccctgatgtc taaacgtatc ctgcattatg caaaggtaga atgcagtttc ttcataaacg
      421 aaagtgagat atcctgattt gttaataaaa gtgaaagcgg aaaatagtac tggtttccca
      481 taaattccgt tttattttta ctttataaaa aatcattgta agattagcag gctttgtgct
      541 acctatttca gaaatataga attatgaaaa acagctttat ttgcttactc gtttttgtgt
      601 acagtatttt aaccgtcaaa agcaatacaa tcagtggcag tatattagac gaactacaaa
      661 aacctttgga attttgcaat gtacttttac atcaatataa tgacagtatt tttttagaag
      721 gtactacatc agacagtaca ggaagttttg atttcaaaaa tattaaaccg ggaaattatt
      781 tcatcgaaat aatttatatc gggtacaaaa cagatacgtt aaataatatc caagtaacgc
      841 aagataagga tgtggattta gccccggtaa cgatgcaacc ggatgctaca gttttaggca
      901 atgtagaaat caaatctact cgtccggtga tagaacgcaa agcagaccga cttgtctata
      961 atgtagaaaa cagcacaaaa tcaagtggtg aaaattcatt tgatttatta cgcagtgtgc
     1021 cgggtgtcag cattacaggg aatgaccaga ttaatgtaaa cggaaaaagt ggagtgcaag
     1081 tgatgataaa tggcagagtg gaaaatttga gtggcgacca gctggttact ttcttgaaat
     1141 ctatccagag ttctaacatt aaaaagattg aagtcatcag caatccgtca gcaaaatttg
     1201 atgctgaagc aaagggcggc attattaata ttcagcttaa aaacaacagc cgttcgggag
     1261 taaacggttc tgtttatgtt aactacagac aaaataaata tgcatccact gaagcaggat
     1321 ttaacttaaa cgtgaattat aaaaaatggg ttgtgagtat ggcatataac aatagctatg
     1381 gcaaaggtta tcaggatcac atattcacca gagattttac ctttaatgac agtatacagc
     1441 aatatgatga aaagggtaag gatgttaacc gttttatggg acattttggc aacctgcaaa
     1501 tcaactataa catcaatgac aaacacacaa ttggcatcac ctcagaaact ttttgattct
     1561 cagacaacca tattggcaat tcaatattaa aaatatatga taatattgtc aaaaacaatg
     1621 aattttcgga tattcaaaaa acgaataata ccacgaaaac atccggtgtg agtcccacag
     1681 taaattttca ttatatcggc aatttggata catccggcat caagctcgaa ttcaattatg
     1741 actttgcatt tttccgtttc ttcacaaaca gctcattaat gacagatgac tatgataaac
     1801 aggaaaatta tataagtaac ttatacaatt tcactcaaaa caatcctttt attgtgcatc
     1861 tgcatacttc taatttaaat tattacaaac cgctcaagaa aggacataat attgaaaccg
     1921 gtttcaaatt tacctggacc aaaaccggaa atgatgtaca gtttaaaaac tgggatggga
     1981 ctcaatatat attggatgcc gggaaatcta acaagtttga gtatatcgaa aatataaatg
     2041 ccctttttgg aatatggagt aaagaatgga aaaagaactg gtcgactaac ttaggcttgc
     2101 gtatcgaaca aaccaatacc aaccagtatt ctgaaacagt aaacagcaga acgaaaaaac
     2161 attatgtcga tttttttcca agtttattta ttcaaaagac cataaaagaa aaacacagtc
     2221 ttaatatcaa tttcagccgg aaaattcagc gacctaattt tgaggatttg aatccatttg
     2281 agtattatct aagcccttat tccatctgga ccggaaatgc caatttaaag cctcaattag
     2341 tcaacttaac cgaaataact tacaccttca aaaatactta cagctttttt atcaatcatg
     2401 aaaacctgaa taacaattca acccatttgg tgtttcagga tgacagtaca aaaatatcca
     2461 cctaccgtgc ttctaatttc agggtgcgca ataacctgaa tattggacta agtatcaata
     2521 aagacttgtt taaatggtgg tcaatttctg ccaatgcgac ctatacgttt ttcagataca
     2581 attccattgt gaatgacgaa gaatttaaag tcagttctca taaaggcaac ttttctgtag
     2641 acaatacatt cagactacct aaagattttc agatcaatgt ctttgccttt tatactacgc
     2701 cacatttgga tgccaccgat ataatgaaaa gtgatgggat ggtcaatatt tccatttcaa
     2761 aatcattttt gaaaaaagcc cttaaaatta aactatccgg aaatgatatt ttccgcacga
     2821 aaaacttttc ttacaccact gatttcttca atgtacattc tgtatccatc aataaattca
     2881 gttcagcctt tgtaggatta acagttagtt acaattttaa taaaggcaaa caatttaaaa
     2941 acacggtaat cagcaaaagc aatgaagatg aaaaaagcag aattcattaa tttgttttgc
     3001 aactaatttc ataatctaaa cgaaatctaa tagtaatatt gataaaaact gaatttcgaa
     3061 cgaatgctgt aatttcattt ttttaaaaac aaggatatca tgctatattg aatcagataa
     3121 tcaggcggca taactgatgc tgattttaca acatctggta tttaaactaa aaaaaagatt
     3181 atgagcaatt taaatatttt accgttaaaa gactggggtt ttaattttga aagaccgatt
     3241 attatttcag gtccatgttc gtgtgaaacc gaagagcaaa tgctggaaac tgctaaaaga
     3301 ctagcagata aaggtattga tgtactccgt gcaggtatct ggaaaccgcg cactcgcccc
     3361 ggcatgtttg aaggcatagg aaaagatggt ctgacttggc tgatagaagc cggtaaacaa
     3421 attggcaaac caaccacggt ggaagtggct accgggcaac atgtggaaga tgcactgaaa
     3481 gccggtgttg atattctatg gattggcgca cgtactacag ttaacccgtt ttctgtacaa
     3541 gaagttgctg atgcattgaa aggcgtagat attcctgtat tgattaaaaa tccgataaac
     3601 cccgatctgc aattatggct gggcagtatg gagcgcatta atgccgtagg aataaaacgc
     3661 atcggtgcta ttcatcgcgg attttcttct tatgaaaaat caaaatacag aaacaaaccc
     3721 atgtgggaga ttccggtgga gatgaagcgt ttatacccgc acatgccaat tattgtagat
     3781 ccttcacata tttgcggagc tcgagaatta attccttcag ttgcacaaaa ggcactcgac
     3841 cttgactttg acggattgat gatagaatct caccgcgacc ctgaccaagc gtggagtgat
     3901 gcccaacaac aagtaacacc ggaacgactg ggtgaaataa tttcgggctt gatagtaaga
     3961 cacagcaaac tggatgatta tgaagaaaac aatctggaag ctttgcgtgc tcagattgac
     4021 agaatagata attatatgtt ggaaattatg ggcgagcgca tggatattgc ccgcaaaatt
     4081 ggtgaattta aacgcgacaa cggcatcacc attttacaaa caaaccgttg ggatgaaatc
     4141 atcaacgaca ggataaaaaa agcctctaaa aaagaattga cagaagattt tgtacgagag
     4201 atgatggatg ccatacatca ggaatctata cgtcatcaga ctaaaatcat gaataatatt
     4261 ttggctgaat aaagaaaaaa gcaggaataa attgagatat aatttatcgc ttggtgtctt
     4321 caccaagcga ttttgttttt agtcaaactg cagtagcctt agtttggtta aagtcacagc
     4381 aataaaaagc tctgcctttg ttggacaaat taaaatggga aagtagttaa atttaattgc
     4441 attcgaaaga tagcaacaat ataaaatgcg aagcaccaaa aatactgaaa tatacggaat
     4501 gcgcatacgc attttgtata tttttttaga taatgtcaag attatcaacg ttttttgtaa
     4561 aaataaataa aaaaaagtaa aaagttgtat tgacgaataa aaattgcgta tatttgggag
//
LOCUS       DAOQII010000206         4612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11107_length_4612_cov_6.280228, whole genome shotgun sequence.
ACCESSION   DAOQII010000206 DAOQII010000000
VERSION     DAOQII010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11107_length_4612_cov_6.280228"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1171
                     /locus_tag="PLF48_09350"
     CDS             314..1171
                     /locus_tag="PLF48_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49574.1"
                     /translation="MSIFKNMRWVAEDFLYLLQSHTKVFVPFVFWCAGIAYAVYKSVQ
                     YMNTHAHVEDFIGFILNSSFAFILFNLLLCPILVISNLWVLNILKQANNQEEISVGSA
                     FLHAIGFKMIMLLPFVLIWMGLQFLFICIFAIVMFLAGLSRGNIGGSIIGKLIGGLFT
                     IVLFMTFFLYMVTPGMVLDDKNMFSSARQMMRYIKKYAAVFKAKFFAVLSLYLLLTLG
                     LYGLLNLFTGDNGDFDSVFVGIYLPIVSLIFIYYLQIKAGQFYIWSIIQDSKGVVTFE
                     PINQINDAH"
     gene            1158..2348
                     /locus_tag="PLF48_09355"
     CDS             1158..2348
                     /locus_tag="PLF48_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNF49575.1"
                     /translation="MMLTNRQLFLQHIAQTSDAPLALEIVNANGMYLTDADGKKYMDL
                     ISGISVSNLGHCHPRVVEAVQTQAATYMHTLVYGEFVLSPQVKLAEYLCSLLPKNLNS
                     VYFTNSGAEATEGAMKLAKRYTGRTELISCIHSYHGSTQGALSILGDEYFKSAFRPLL
                     PDTRLIRYNSEEDLSFISKRTAAVFIEPVQAEAGILVPDKFYLQKVRQRCNETGTLLV
                     FDEIQTGCGRTGTLFRFEKENVIPDILLLAKGLGGGMPVGCFISDKKIMNTLTYQPVL
                     GHITTFGGNAVCCAAALASLKAIQEDRLYASVEAKEKLFVELLKHTAIKKVTHCGLML
                     AVHLDNFENLRKVIQLCIENGVITDWFLFCDNALRIAPPLIITEQEIRQACVIILEAL
                     DKIY"
     gene            2407..3171
                     /locus_tag="PLF48_09360"
     CDS             2407..3171
                     /locus_tag="PLF48_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNF49576.1"
                     /translation="MKKLFFIICMTMMFNNLFSQNLLAANSGSSTEKYIEVQVTDTMM
                     VIPDMVTLMVKIDEEEEEVNYFDENFDDKKNKKTKPTETNNSQSSKDKAEAVFKKYNL
                     TEFTFHEKSEGKNPFDKEFSIYENAYEVKVKNLAILENLMKDLKEIENTESLVTGSQL
                     TDKFSYELILIEKLMQKAEREAKAIAKAMNVTLGKPLNVSNQSMTNINSSMFNNTENM
                     GGLGAMFNMLGNMFKSQNGETKVTISKSLVVRYSYQ"
     gene            3222..4334
                     /locus_tag="PLF48_09365"
     CDS             3222..4334
                     /locus_tag="PLF48_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF49577.1"
                     /translation="MLYKRMPIEVESPEEMGYSTILYNLAESSVRDVYFKDLKLDLNE
                     LFLCYGEHRGDIKLRKAIVKSEKDLNENHVLVCPSAATALFIVSTTLLTERDHLIVLR
                     PNYATNIETPRAISCEMDIVDLYFENGYALSADVILSRIKPNTKLISLTTPHNPTGVV
                     LDDTVVKEIIDVARSKNVIVLVDETYRFLNFQSDLIPYYAAYADNVMSVCSLSKAHGV
                     PGIRTGWLLCKNEQLMYSFLAAKEQVIITNSVVDEAIALHILQQNEDYLKPVHAHIRN
                     NFSIIKTWMKEHEFLDWVEPGAGVVAFPRIKNAKMLDLKKFYTLLYENYKTLVGAGHW
                     FEQSDACFRLGFGYPSKEELIQGLENIDNALRDSVL"
     gene            4365..>4612
                     /locus_tag="PLF48_09370"
     CDS             4365..>4612
                     /locus_tag="PLF48_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49578.1"
                     /translation="MTKSITYTSKMDTALMDELTTYSKRLNKNKKELIEAALRNYFIQ
                     IKKLQYAESYKRANADAEIKEWADLGLDDYQQQLEKTEG"
BASE COUNT         1534 a          729 c          898 g         1451 t
ORIGIN      
        1 cgacagaggc agttttgctt tatacttgcc atagtattca aggagatcat tactgatgtc
       61 gagttttgct gcaatctctt tgatgtgaat aataggattt tttctgacga tttcgagatc
      121 ggtaggaaat tctggcatac tttatttttt ataaaggtaa agttttaagc aagttcagga
      181 aatgacagaa agcatatttt tatctgtttt ttaaatgtaa aaatgggaat gattcagaat
      241 caaacaagca gatttatttc attcaaaaaa tgtaatataa gattttatat tgagaatatt
      301 gtacatttac aaaatgagta tttttaagaa tatgcgttgg gtagccgaag attttctgta
      361 tctgctacaa agtcatacta aggtttttgt accttttgta ttttggtgcg caggtattgc
      421 ttatgcagtc tacaaatcag tacaatatat gaatacacac gcccatgtcg aagattttat
      481 aggatttata ttgaattctt ctttcgcttt cattctcttt aatctgttgt tatgccccat
      541 cttagtcatc agcaatttat gggtattgaa tatcttaaag caagcaaata accaagagga
      601 aatatctgtc ggatctgcat tcttacacgc tatagggttt aaaatgataa tgcttttgcc
      661 atttgttctt atttggatgg gcctgcaatt tctattcatc tgcatattcg caattgtcat
      721 gtttcttgcc ggtttgtcaa gaggaaatat tggcggcagt ataattggaa agttgattgg
      781 cggtttgttt accatcgttc tttttatgac atttttcttg tacatggtaa cgccgggcat
      841 ggtgttggac gataaaaata tgttttcatc agcaaggcaa atgatgcgat atatcaaaaa
      901 gtatgcagcc gtgttcaaag ctaaattttt tgcagtattg tccttatatc tattactcac
      961 ccttggatta tatggtttat taaatctttt tacgggtgat aacggagatt tcgatagtgt
     1021 tttcgtgggg atttatttac cgatagtatc tttgatattt atttattact tacaaatcaa
     1081 agcaggtcaa ttttatattt ggtctataat acaagattca aaaggcgttg ttacctttga
     1141 gccgataaat caaattaatg atgctcacta atcggcagct ttttttacaa catatcgcac
     1201 aaacatcaga tgcgccttta gccttagaaa tcgtgaatgc caatggtatg tatttaaccg
     1261 atgccgatgg aaaaaaatac atggatttaa tttccggcat ttcagtaagt aatttaggtc
     1321 attgtcatcc cagggtagta gaagcagtcc aaacacaagc agccacttat atgcatacgt
     1381 tggtttatgg cgagtttgta ttatcgcctc aagtaaaact ggctgaatat ttatgcagtt
     1441 tgttgcccaa aaacttaaac agtgtatatt ttacaaacag cggtgccgaa gccacagaag
     1501 gcgctatgaa attagctaaa cgctacaccg gaagaaccga attaatttca tgtatccact
     1561 catatcacgg cagtacgcag ggagctttga gtatattggg cgatgagtat tttaagtctg
     1621 cattcagacc acttctaccc gatacaagat tgatacgtta taatagtgaa gaagatttat
     1681 ctttcatttc aaaaagaaca gcagctgtat ttatagagcc ggtgcaagcc gaagccggca
     1741 tacttgtacc tgataaattt tatttgcaaa aggtaagaca acgctgtaat gaaacaggga
     1801 cactgctcgt atttgatgag atacaaacgg gatgtggcag aacaggtact ttattcagat
     1861 ttgaaaaaga aaatgtaata ccggatatat tattgctggc aaaaggctta ggtggaggta
     1921 tgcctgtagg ctgttttatt tcagacaaaa aaattatgaa caccttaaca taccagcctg
     1981 tacttgggca tattaccact ttcggaggca atgcagtatg ttgtgctgca gcattggcaa
     2041 gtttaaaagc tattcaagaa gatcgtttat atgcttctgt tgaagcgaaa gaaaaattat
     2101 tcgttgaatt attaaaacat actgctataa aaaaagtaac acattgcgga ttgatgctgg
     2161 cagtacacct tgataatttt gaaaatctca ggaaagtgat acagttgtgt attgaaaatg
     2221 gtgtcattac agactggttt ttattttgtg ataatgcttt gcgaattgca ccaccactta
     2281 ttattacaga acaagaaatc aggcaggctt gcgtaattat tttagaggcg ttagataaaa
     2341 tttattgaat ttggtgtttt atttaattat atactttata tatttgtaca taaatttctt
     2401 tcaaaaatga aaaaactatt ctttataatc tgcatgacta tgatgtttaa caacctgttt
     2461 agtcaaaatc ttcttgctgc aaactcaggc agttcaacag aaaaatatat cgaagttcag
     2521 gttacagata caatgatggt tattcctgat atggtaacat taatggtaaa gatagacgaa
     2581 gaagaagagg aagttaatta ctttgatgag aattttgatg ataagaaaaa taaaaaaacc
     2641 aaaccaacag aaacgaataa ttcacaatca agtaaagata aggctgaggc cgtttttaag
     2701 aaatataatt taacagagtt tacctttcat gaaaaatctg aaggtaaaaa tccatttgac
     2761 aaagaatttt caatttatga gaatgcttat gaagtaaaag tcaaaaattt ggcaatactc
     2821 gagaatctaa tgaaagattt aaaagagatt gaaaatacag aatcgttagt aacaggttca
     2881 cagcttactg ataaattcag ttatgaacta atattgattg aaaaattaat gcaaaaagct
     2941 gagcgagaag cgaaagcgat tgcaaaagcg atgaatgtaa ctttaggcaa gcccttaaat
     3001 gtcagcaatc aaagtatgac taatatcaac tcttcaatgt ttaacaacac ggaaaatatg
     3061 ggtgggctgg gcgctatgtt caatatgctt ggtaacatgt ttaaatctca aaacggtgaa
     3121 acaaaagtta ccatctccaa gtcattagta gttcgttaca gctaccaata attataaaca
     3181 aggcaaacta ttatttcttt gcatttacgt acctttagaa aatgctctat aaacgtatgc
     3241 cgattgaagt agaatcgccc gaggaaatgg gctattctac catcttatac aatcttgccg
     3301 aaagttcggt acgcgatgtg tactttaaag atttgaaact ggatttgaac gaattgtttc
     3361 tttgttatgg agaacatcgt ggcgatatca aactccgcaa ggcaatagta aaatcagaaa
     3421 aagatctgaa tgaaaatcat gtgttggttt gtccaagtgc tgctactgct ttatttattg
     3481 tgtccaccac attattaaca gaacgcgatc atctgattgt attgcgacct aattatgcta
     3541 caaatattga aacaccacgg gcaatttctt gtgagatgga cattgtagat ttatactttg
     3601 aaaacggata cgcactttcc gctgatgtta ttttatccag gataaaaccg aatactaaac
     3661 tgataagttt aaccacgcca cacaacccaa ccggagtggt tttggacgat actgtagtaa
     3721 aagaaattat tgacgtggca cgctccaaaa atgtaattgt tctggtagat gaaacgtatc
     3781 gcttccttaa ttttcaatcc gatttgattc cctattatgc ggcttatgcc gataacgtga
     3841 tgtcggtttg ttccctttct aaagcgcatg gtgtgcccgg catacgcacg ggttggttgc
     3901 tgtgtaaaaa tgagcaattg atgtattcct ttttggcagc caaggaacaa gttatcataa
     3961 cgaattcagt agtagatgaa gcgatagcct tgcatatttt gcaacaaaat gaggattacc
     4021 tgaagcctgt tcatgcgcac atacgaaaca atttttccat tataaaaaca tggatgaaag
     4081 aacatgaatt cttggattgg gtagagcctg gcgcgggagt cgtagccttc cctcgcataa
     4141 aaaatgcgaa gatgcttgat ttgaaaaaat tctatacgtt attgtatgaa aattataaaa
     4201 ctttggtcgg tgccggacac tggtttgaac aatccgatgc atgtttccgc ttaggtttcg
     4261 gatatccgtc aaaagaggaa ctaatacagg gattggaaaa tattgataat gccttgcggg
     4321 attctgtttt ataaactata tttacagatt gtaaatacta aataatgact aaaagtatta
     4381 cctataccag caagatggat actgcactca tggatgagtt gacaacgtat tcaaagcgac
     4441 ttaacaaaaa taaaaaagaa ctgattgagg ctgccctaag aaactatttc atacaaataa
     4501 aaaaactgca atatgcagag agctataagc gggcaaatgc ggatgcagaa ataaaagaat
     4561 gggctgattt gggtttggat gactatcagc aacaacttga aaaaacagag gg
//
LOCUS       DAOQII010000207         4562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11287_length_4562_cov_4.071001, whole genome shotgun sequence.
ACCESSION   DAOQII010000207 DAOQII010000000
VERSION     DAOQII010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_4562_cov_4.071001"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3632
                     /gene="porU"
                     /locus_tag="PLF48_09375"
     CDS             <1..3632
                     /gene="porU"
                     /locus_tag="PLF48_09375"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNF49579.1"
                     /translation="FYAFTLENDGQAYDFSISDEVFATIDQNKLTENQKKIIKNQVLI
                     QQFSGKSGLKTIHTFQVLPFRLNNGNYEILTSFNLHYQASASTQKNSISNERIYPTHS
                     ILQQGTFYKIGIEKEGIYKLDYNFFKNLGINIDQVNPNNIRIFGHRGGMLPELAGADC
                     ETDVVELPIKIVVANSSRVQNGDYVLVYLPGPDTWKYNTSSQLFTATKHLYSDKQYFF
                     ITTDNGTNKIISSMGSSAQTPSQTFTTYNDYAFIEEEKVNIAHSGRIFLGDEFGSTPS
                     KNYTFSFPNINTAYPLKANLAVASNSMTYGSSFSLNFNGNVQTLNMGNVIEPYPGIFN
                     PAVYKQQLFTYPNPVSSPQFTLSFERNGDFNTKGWLDFLQVNAISDLKYNGSSFLFRN
                     AASAGTGNVSKFIIQNMSPNVEIWDITNPFEVKKINFNVSGNIADFTIATDSIKQFAV
                     VDNSGLTPTAYGKINNQDLHAMPQQDMLIITRKSFLTQAEQIAQFHRSKQGLSVAVVE
                     LEKIFNEFSSGTTDITAVRNFIKMFYDRANGNTNLMPKYVLLFGDGTYDNKNLGDYFL
                     PTYESKSTFEALSTYVSDDYFGCLNDNEGADITNTALNIMDVAVGRIPVDNTDKANNA
                     YAKILSYSSKAAMGEWRNQSTFIADDEDYNLHFDDAEDIANSYTSQYKCTNTDKIYLD
                     AFNQQSGTGGARYPDVNATINRKFYSGSLFFNYMGHGGPLGLAQEGILSTVDIDSWDN
                     PLKLPFFITATCEFTPFDDFHIYSAGERMLLRSGGGAIGLLSTTRLVIATQNKLINQN
                     FMQKMVDATQNINMTIGDITREAKKITTSTYEGNRKFSLFCDPALRPAFPFHHCVVSM
                     IDSVPVTLPTDSIKALSKIRISGEVQDRVSHTLLSRFNGVVSVIVYDKPKTYYTLQND
                     PQSSVDDFIMQKNILYKGRTKVVNGKFEVQFLAPRDIDYTYGLGKISLYAYNDSTDAC
                     GYYDQIVVGGAYDTTIADNVGPDVDLYMNDDKFVFGGITDNNPLLFAKLYDENGINTS
                     GSGIGHDITAVIDNDMRNVYTLNDFYETDADAISKGSVKYPFAGLKEGRHTLKLTAWD
                     ILNNSGSGYTEFIVEKSADLALAHVLNYPNPFTTGTNFMFEHNKPGQDLTVKIEIFSV
                     SGKIVKTILKNINTEGYRVNDISWDGKDDFGDKIGRGVYIYRITVKDDTGKKSSQYQK
                     LVVLN"
     gene            3692..>4562
                     /gene="porV"
                     /locus_tag="PLF48_09380"
     CDS             3692..>4562
                     /gene="porV"
                     /locus_tag="PLF48_09380"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNF49580.1"
                     /translation="MMKLKNFNKNVLLIVGVMACFSLSAQVNYKKGGINTVNSAVPFL
                     RIVPDARGGAMGDVGIATSVDPNTSYYNASKLVFAKRKLGLSLSYAPWLRALGITDIY
                     LAHFSMYYKVSRNDVVHTSLKFFSLGQIDYTDIDGNSLGTGRPNELTVDAGYSRRLGK
                     GIGLGVAFRYIYSNLAKGQTTPGSSEIIRAGHAFGADVSFTYTKDLKMKNEKMKSNIM
                     VGAAVTNIGTKISYLKSSKEKDYIPTNFGVGIGYRIQPNKYHEVGIYADFNKLLVPTP
                     DTLDNNNDGIADFK"
BASE COUNT         1485 a          868 c          863 g         1346 t
ORIGIN      
        1 cattttatgc atttactctg gaaaatgacg gtcaagcata tgatttttct atttcagatg
       61 aagtttttgc cacaattgac caaaataaat taacagaaaa tcagaaaaaa atcatcaaaa
      121 accaggtgct tatccaacag ttctccggaa aatccggact taaaacaata catacctttc
      181 aggtattgcc atttagattg aataatggaa attatgaaat actgacctct tttaatctgc
      241 attatcaagc atcagcttca actcaaaaaa acagcatttc taacgaaaga atttatccta
      301 cgcactccat tttgcagcaa ggcacttttt ataagatagg aatagaaaaa gaaggcattt
      361 ataaactgga ttataatttt tttaaaaatt taggcatcaa tattgaccag gtcaatccca
      421 ataatatcag aatattcggg catcgaggtg gtatgctgcc cgaacttgcc ggggcagact
      481 gcgaaaccga tgtagtagaa cttccgatta aaatagtggt agccaacagc agtcgtgtac
      541 aaaacggcga ttatgtattg gtttatttac ccggtccgga tacatggaaa tacaatacgt
      601 cctctcaact ttttacagcc accaaacatc tttacagtga taaacaatat tttttcatca
      661 cgacagataa cgggactaac aaaataatca gcagtatggg ttcatctgca caaacaccct
      721 cacaaacttt tactacatat aatgattacg cttttataga agaagaaaaa gtgaatatag
      781 cacactccgg cagaatattt ctcggtgatg aatttggcag tactccttct aaaaattata
      841 ccttttcttt tcccaatatc aatactgctt atccgctaaa agccaattta gcagtagcat
      901 caaattcaat gacatacggc tcatcctttt cattaaattt caacgggaat gtacaaacac
      961 tcaatatggg taatgtaatt gaaccctatc ccggaatttt taatcctgcg gtgtataaac
     1021 aacagctatt cacatatcct aatccggttt ccagccctca gtttactttg agttttgaac
     1081 gcaacggtga ttttaataca aaaggttggc tcgatttttt acaagtgaat gccatctccg
     1141 atttaaaata taacggctcc tcatttttat tcagaaatgc tgcttctgca ggcacgggta
     1201 atgtgtcaaa atttatcata caaaacatgt cgccgaatgt tgaaatttgg gacatcacca
     1261 atccatttga agtaaaaaaa attaacttca atgtgtccgg taatatagct gattttacta
     1321 ttgcaaccga ctccataaaa caatttgcag ttgtagataa ttcgggacta acacctacag
     1381 cttacggcaa aattaacaat caggatttac atgcaatgcc acagcaagac atgctcatta
     1441 ttacccgtaa aagttttctt acccaagctg aacaaattgc tcaatttcac cgaagcaaac
     1501 aaggcttatc agtagcagtt gttgaactcg aaaaaatatt caatgaattt tcatccggaa
     1561 ctacagatat cacagccgta cgcaatttta taaaaatgtt ttatgacaga gcaaacggca
     1621 ataccaactt gatgccaaaa tacgtcttgc tttttggcga tggcacgtat gacaataaaa
     1681 atttaggcga ttattttttg cccacttatg aaagcaaaag tacgtttgaa gccttatcta
     1741 cttatgtctc tgatgattat tttggctgtc tgaatgataa tgaaggagcc gatattacta
     1801 atacggcatt aaatatcatg gatgttgccg taggacgtat tccggtggat aatacagata
     1861 aagcaaataa tgcttatgct aaaattttat cttattcttc caaggcggca atgggtgagt
     1921 ggcgaaatca atctacattt atcgctgatg atgaagatta taacttacat tttgatgatg
     1981 ctgaggatat tgccaacagc tacacttcac aatataaatg tacgaatacc gataagattt
     2041 atctggatgc tttcaaccag caatcgggta caggtggcgc acgctacccg gatgtaaatg
     2101 caactatcaa ccgcaaattt tactccggct cattattttt taattatatg ggacatggtg
     2161 gcccgcttgg attggcacaa gaaggtattt tgtctaccgt ggatattgac agttgggata
     2221 atccgcttaa acttccattt tttatcacag ccacttgtga gtttactcct tttgacgatt
     2281 tccatatcta ctctgccgga gaaagaatgc tgctgcgtag cggtggtggt gctatcggtt
     2341 tgttatctac tacacgactg gttattgcca cccagaataa actaatcaat caaaacttca
     2401 tgcaaaagat ggtggatgca actcaaaata tcaatatgac gattggagat atcacaagag
     2461 aggcaaaaaa aataacgaca tccacctatg agggcaaccg caagttttct ttgttttgtg
     2521 atccggcttt gcgtccggct tttccatttc accattgcgt agtatcgatg atagacagtg
     2581 tgcccgttac actgccaact gacagtatca aggccttgtc aaaaatcaga atcagcggtg
     2641 aagtacagga tagagtaagt catactttgt tatccagatt taatggggtg gtgtctgtaa
     2701 ttgtctatga taaaccaaaa acatattata ctttgcaaaa tgatccgcaa agtagtgtag
     2761 atgattttat catgcaaaaa aatatattgt acaaaggcag aacaaaagta gtaaatggca
     2821 aatttgaagt acagtttctt gctccccgtg atattgacta tacatatggc ttaggcaaaa
     2881 tcagtttgta tgcctacaat gacagtacgg atgcatgcgg atattacgat cagatagtag
     2941 taggtggtgc ttacgacact accatagcgg ataatgttgg acctgatgtt gatttataca
     3001 tgaatgacga caagtttgtc tttggcggca tcacagataa caatcctttg ttgtttgcca
     3061 aattatatga tgaaaatggg attaacacct ccggttcggg catcgggcat gatattacag
     3121 cggtaattga taatgacatg cgcaatgtgt atacactcaa tgatttttat gaaacagatg
     3181 cagatgccat ttccaaaggc agtgttaaat atccgtttgc cggcttgaaa gaaggaagac
     3241 atacgttaaa actaaccgct tgggatatat taaataattc aggttcggga tataccgaat
     3301 ttatagtaga aaagtctgca gatcttgcac tggcgcatgt actcaactat ccgaatccgt
     3361 ttactaccgg tacaaatttc atgtttgaac acaataagcc cggtcaggat ttgacagtaa
     3421 agattgaaat tttcagcgta agcggcaaga ttgtaaaaac aattttaaaa aatatcaaca
     3481 cagaaggtta tcgtgtgaat gatatttcct gggacggtaa agacgatttt ggcgataaaa
     3541 tcggcagagg tgtgtatatt tacagaataa cggtaaaaga tgataccgga aaaaaatcat
     3601 cacaatatca aaaattagtg gttctaaatt agtttattta aaataaattt ggcaatttaa
     3661 cgttttaaaa ctcgacaata aaattaggtt catgatgaag ttgaagaatt tcaataaaaa
     3721 tgtgttgctc atagttggtg taatggcatg tttttctctc tctgcacagg taaactataa
     3781 aaaaggcggc attaatacag tcaacagtgc agtacccttt ttgcgtattg ttcccgatgc
     3841 ccgtggcggt gccatgggag atgtgggaat tgctacatct gtcgacccga atacttcata
     3901 ttataatgcc tctaaacttg tatttgccaa acgtaaatta ggcttatcgc tatcgtatgc
     3961 accctggctt cgtgcactgg gcattaccga tatttattta gctcattttt cgatgtatta
     4021 taaagtatcc cgcaatgatg tggtacatac ctctctgaaa tttttcagct taggacaaat
     4081 tgactatacc gatattgacg gtaattctct tggcacaggc agaccaaatg agctgactgt
     4141 tgatgccggc tactcgcgtc gcttgggtaa aggcataggc ttgggtgtgg cattccgcta
     4201 tatttattcc aatctggcta aagggcaaac tacaccgggc agttcagaaa ttatcagagc
     4261 cgggcatgca tttggagctg atgtcagctt tacttacacc aaagacctga aaatgaaaaa
     4321 tgagaaaatg aaatccaata tcatggtggg cgctgccgtt accaatatcg gaactaaaat
     4381 ctcttacctc aaaagctcta aagaaaaaga ctatatacct actaactttg gtgtcggcat
     4441 tggttaccgc atacagccca acaaatatca cgaagtgggt atttacgccg atttcaataa
     4501 gctcctggta cctacaccgg ataccctcga caataataat gatggaattg ccgatttcaa
     4561 ac
//
LOCUS       DAOQII010000208         4561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11288_length_4561_cov_5.669108, whole genome shotgun sequence.
ACCESSION   DAOQII010000208 DAOQII010000000
VERSION     DAOQII010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11288_length_4561_cov_5.669108"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLF48_09385"
     CDS             complement(<1..350)
                     /locus_tag="PLF48_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF49581.1"
                     /translation="MQTNNEIKDMVKQKYSEIAMQNKTENASSCCGSSISSTEVYNIM
                     SDEYCHVEGYNPDADLKLGCGLPTSFAKIKQGDTVVDLGSGAGNDCFVARHETGDRGK
                     VIGIDFTENMITKA"
     gene            complement(367..696)
                     /locus_tag="PLF48_09390"
     CDS             complement(367..696)
                     /locus_tag="PLF48_09390"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF49582.1"
                     /translation="MGASKTDHFSAEHNRMANLAKAMGHPARIAIIEYLLQTEGCICG
                     DIVNVLPLSQPTVSQHLRELKNAGIIQGSIDGNAICYCVNETTIATLKHFIDQISKKI
                     IEKKSCC"
     gene            1004..2722
                     /gene="rho"
                     /locus_tag="PLF48_09395"
     CDS             1004..2722
                     /gene="rho"
                     /locus_tag="PLF48_09395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNF49583.1"
                     /translation="MIYHADNLNDLALADLKSIAEQLLIPKTKIAKTKKPDLIELILK
                     KQEMNKTDNQELLQDQTAETKTEENTETDTTESGEKRKRARIPKPKVEKVEAVSKPQQ
                     TEIKGHDTFPWKEKTNETTEQKTTEEEVKSTLDNEEKVSIFHREDAVPVKKQHKFEKK
                     EKFQKHRDKPEPTENSAEEGKEAANTSEMVSEEKKDEFNIELDGIISGEGVIEIIPEG
                     YGFLRSSDYNYLSSPDDVYVSPSQIKSIGLKTGDTIVGTIRPPKEGEKYFALLKVDLI
                     NGKSPSEIRDRIPFDYLTPLFPNEKLDLFMDASTYTTRMVDLFAPIGKGQRGMIVAQP
                     KVGKTMLLKDIANSIAKNHPEVYLIILLIDERPEEVTDMQRSVNAEVIASTFDEPAER
                     HVKVSNIVLQKAKRLVECGHDVVILLDSITRLARAHNTVAPSSGKVLSGGVEANAMQK
                     PKQFFGAARKIENGGSLTIVATALIETGSKMDEVIFEEFKGTGNMELQLDRRLANKRI
                     FPAIDLVASSTRRDDLLLDKDTQSKMWILRNHIADMNSEEAMKFLLNRMKGTRSNEEF
                     LLTMNR"
     gene            2815..3483
                     /locus_tag="PLF48_09400"
     CDS             2815..3483
                     /locus_tag="PLF48_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49584.1"
                     /translation="MKKCLSISLILFLMLAVNANAGENKLTKALNDGIEQEDIEATVV
                     DNLLSVEFLLTNTGLRYERKLADRHALAVSAGLGFPFSVSASKVYYSNGTSKSSTSAY
                     IAVLPQVGIAYRFYYNLQRRINRDKSVYANSANYFTVLSSFGFTPIYSNYPTKFSFIT
                     NIAPAWGMHRTFIKSNMDINFNIGLGYGYNNGSGSYVAPILNFTFGYVVLPKHKKIKS
                     ATTE"
     gene            4088..4276
                     /locus_tag="PLF48_09405"
     CDS             4088..4276
                     /locus_tag="PLF48_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49585.1"
                     /translation="MAEPILVIKELEYWGMLSFQFTYTSLMMINFQKLMILENKSEFT
                     FNHYDSWLMMNFKLTEKH"
BASE COUNT         1561 a          810 c          849 g         1341 t
ORIGIN      
        1 ctagcttttg ttatcatgtt ttctgtaaag tcgatgccta tcactttgcc tcgatcccct
       61 gtttcgtgtc gggcgacaaa gcagtcatta ccggctccgc ttcccaaatc aactacggta
      121 tcaccttgct taattttcgc aaaagaagtt ggcagaccgc atcctaattt taaatctgca
      181 tctggattgt acccctccac gtggcaatat tcatcactca ttatgttata cacttcagta
      241 ctagagatgc tgctgccgca acaagaggat gcattttcgg ttttgttctg catggcaatt
      301 tcgctgtatt tttgtttcac catgtcttta atttcgttat ttgtttgcat tgttttatat
      361 ttataattaa caacaagatt tcttttcaat tattttttta gagatttggt caataaaatg
      421 cttaagtgtt gcaatagtag tttcatttac acaatagcaa atagcatttc catcaatact
      481 accctgaatt atacctgcat ttttcagctc acgcaaatgc tgagatacag ttggctgaga
      541 taacggcaac acattaacga tatccccaca gatacagcct tctgtttgta aaagatattc
      601 aatgatggca attcttgccg gatgtcccat tgccttggcc aaattagcca tcctattgtg
      661 ctcagctgaa aaatgatcgg ttttagaagc gcccatatct ttattttaat attgcaatat
      721 tacgataaag atttttaata aacaactttt tatggaacat taatactttt ttttgaaaat
      781 gggaatacta tacagatttg atacattctc gttaagtatc cgtttctgta atcaatttga
      841 cagtaaaata aagccagaat ctaaaaaaaa ctcaatttat ataaacaatt cagaaaatta
      901 ttgtttattt gcactatcaa tcaatctccc cgctctttat tgagcaacaa agttaaataa
      961 tccgactact taatgttttt ttttcataac taaagatgaa tgtatgatat atcatgccga
     1021 taatttgaat gatttggcat tggcagattt aaaatcaatt gccgaacaac ttttaatacc
     1081 caaaactaaa attgccaaaa ccaaaaagcc agaccttata gagttaatct tgaaaaaaca
     1141 agaaatgaat aagacagaca atcaagaact gctgcaagac caaacagctg aaacaaaaac
     1201 agaagagaat acggaaacag acaccaccga aagcggagaa aaaagaaaac gtgcacgtat
     1261 accaaaaccc aaagtagaaa aggtagaagc tgtttctaag ccgcaacaaa cagaaattaa
     1321 aggtcatgac acttttccat ggaaagaaaa aacgaatgaa actacagaac agaaaaccac
     1381 agaagaagaa gtgaagtcaa cattggataa tgaagaaaag gtatctattt ttcatcgtga
     1441 agacgcagtt ccggtaaaaa aacaacataa attcgagaag aaagagaaat ttcaaaaaca
     1501 cagagataaa cccgaaccaa cagaaaacag tgcggaagaa ggtaaagaag cagcaaacac
     1561 ttctgagatg gtatcagaag agaaaaaaga tgagtttaac atagaactgg acggcataat
     1621 ttcgggagaa ggagttatag aaattattcc tgagggttat ggcttcttgc gctcatccga
     1681 ttataattat ctttcatcac ctgacgatgt ttatgtttcg ccgtctcaaa ttaaatccat
     1741 aggactaaaa acaggcgaca ccatagtagg caccatcaga ccaccaaaag agggcgaaaa
     1801 atattttgcc ttgttaaagg tggatttgat taacgggaaa tctccaagtg aaatccgtga
     1861 cagaattcct tttgattacc tcacgccact tttccccaat gaaaagttag atttgtttat
     1921 ggatgccagc acctatacca cccgtatggt agatttgttt gctcctattg ggaaagggca
     1981 gcgcggtatg atcgtagcgc aacctaaggt gggtaaaacc atgctattga aagatattgc
     2041 caactcgata gccaaaaacc atcccgaagt ttatctgatt attttattga tagacgaacg
     2101 tcctgaagag gttaccgata tgcagcgttc agtaaatgcc gaagtaattg cttctacttt
     2161 tgatgagcct gcagaacgac atgtaaaagt atctaatatt gtattgcaaa aagccaaacg
     2221 tctggtagag tgcggacacg acgtggtgat attattagat tccattactc gtttggcacg
     2281 tgcacataat accgtagcgc cttcatcggg caaggttttg tcaggaggtg tggaggctaa
     2341 tgccatgcaa aaaccaaagc aattttttgg cgctgcccgt aaaatcgaaa atgggggttc
     2401 tttaacgata gtagccacgg cacttattga aaccggctcg aaaatggacg aggtgatatt
     2461 tgaagaattt aaaggtacgg gcaacatgga gttacaacta gaccgaagat tagccaataa
     2521 aaggatattc ccggccatag atttggtggc atcgtctacc cgtcgcgatg atttactatt
     2581 ggataaggac acgcaaagca aaatgtggat tttacgcaat catatagcag atatgaacag
     2641 cgaagaagcc atgaagtttt tactgaaccg aatgaaaggc acacgcagca atgaagaatt
     2701 cttgctgacg atgaacagat aatccaaacc ggatataaaa tagtataggg tatgatatga
     2761 atcatatatt tatatccaat aaaagcgtta tttttgataa actaaatatt tatcatgaag
     2821 aaatgcttat ctatcagctt gattttattt cttatgctgg cagtaaatgc aaatgccgga
     2881 gaaaataaac ttacaaaagc actcaatgac ggcatagagc aagaggacat agaagctacg
     2941 gtagtagata atttgttatc tgtagagttt ttattaacga atacaggact gcgctatgaa
     3001 cgtaaactgg cagaccgtca cgcactggca gtttcagcag ggcttggttt cccgttttct
     3061 gtaagtgcat ccaaagtata ctattctaat ggcacgtcga aatcatcaac cagtgcatat
     3121 attgccgttt tgccgcaagt aggtatagcc tatcgctttt attacaattt gcagcgcaga
     3181 ataaacagag ataaatctgt ttatgccaat tcagctaatt attttacggt gctttcttct
     3241 tttggtttta cgcccatcta ctccaactat cctactaaat tttcatttat taccaatatt
     3301 gcaccggctt ggggcatgca ccgcactttt ataaagagca atatggatat caattttaat
     3361 attggtttgg gatatggata caataatggc tcgggtagct atgtagcacc aatacttaat
     3421 tttacgttcg gttatgtggt attgcccaaa cataaaaaga taaaatcagc tactacagaa
     3481 taattgtaca ttttaaaagc tgaacagtag catgcttttt ttaattgata tgcaatggtt
     3541 ttatacttgg tttcgtttgg tggggacacc aaccgacgaa agaatataaa atgtgtggac
     3601 aataggaata ttacgcaaat agtgtagatt tatgttttgg tcatatttat gaagactctt
     3661 ttatatatac cattcctgtt tttatttagt tgtagtaatc atacaaagcg gacagacatt
     3721 aactcaaatt tagaatcagt cagctattca caaataacaa gtgacagttc aaaatatcat
     3781 actacagagg actcagtgct gattacaaca gagattggtg agagtttgaa gtatagcaaa
     3841 gtagaatata accttatagt tgacaatcat ccagaatttt ttagtgacat tgtacaagat
     3901 cccgatgctt cttactattg tacgtcggat aagaatggtt ttgagagcga gttaggtaag
     3961 gatgaatatt atatgctata tgcttacttt ctatagcaaa aaaatggagt tggcaaatac
     4021 gctgaacgca ggcaaaaact aattgacatt tacacggatc ttaatttctt ataccaatat
     4081 tttcagcatg gcggaaccta ttttggtcat caaagaactc gaatattggg gtatgctgag
     4141 ttttcagttc acatatacaa gtcttatgat gataaatttt caaaaactta tgatattaga
     4201 aaacaaaagc gaatttacat tcaatcatta cgacagttgg ttgatgatga acttcaaatt
     4261 gacggagaaa cattaaggca agataaggca aaaagcaaac aagagttgaa tatgaagatt
     4321 gacaacatcg acaaagctat tatagataat ttttatttgc gacgagcgca ggaatttcag
     4381 tatgtacact atcaatacta ttgacgaaaa aacgacgagt tttggtattc ggcaggtgtc
     4441 cacaccagcc gatagaaaaa ttaacccatt cgtttttgtt tagcgtcttc accaaaatat
     4501 tgaaataaaa cctattacac accggctggc aattttagta tttaacaatt tacacattaa
     4561 t
//
LOCUS       DAOQII010000209         4557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11309_length_4557_cov_5.545980, whole genome shotgun sequence.
ACCESSION   DAOQII010000209 DAOQII010000000
VERSION     DAOQII010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11309_length_4557_cov_5.545980"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PLF48_09410"
     CDS             complement(<1..1365)
                     /locus_tag="PLF48_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNF49586.1"
                     /translation="MANRAIKKVAVLGSGVMGSGIALHFAGIGCEVLLMDIVPFDLKE
                     EEKNNPKARNRIVTDMFNAALKSKPAQAYSAEVTKRVTLGNFDDDMPKIKDCDWIIEV
                     VVERLDIKQSVYQRVEKFRKPGTLITSNTSGIPIHLLEEGLSEDFQKNFCGTHFFNPP
                     RYLRLLEIIPTPRTSPEVVDFLMHYGDKFLGKETVLCKDTPAFIANRVGVYAMAKVFQ
                     LTAELGLTIEEVDALTGPAIGRPKTGTFKLSDLVGNDTGTKVMMGIKENCPNDEQAGA
                     FAVPNYVQFLLDNKFFGNKSKKGYYYKDDKRNSFSLRLDTLQYEPTGKPKFASLDAAK
                     KAESLKEKVNAFIKADDKGAQLVKRSLAGLFAYVSNRVPEISDVLYAIDDALRAGFAW
                     EVGPFQYWDMVGVQKGIDLAEADGLKVADWVKEMLAAGNTSFYKVENGVQQYYDQQSK
                     TYKAI"
     gene            complement(1623..2291)
                     /gene="deoC"
                     /locus_tag="PLF48_09415"
     CDS             complement(1623..2291)
                     /gene="deoC"
                     /locus_tag="PLF48_09415"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNF49587.1"
                     /translation="MNIATYIDHTLLKPTAQETDIITLCEDAKQYGFYAVCVNGCWIE
                     TCNHLLEDSDVKLAVVAGFPLGAGGKFSKIYEVKNAVINGADEVDMVMNIGFAKNGKW
                     DFILNEIKLCKADIGVNVLKVIIETCFLTENEIKKATEIILQSGADFIKTSTGFGTGG
                     ATLSDIAIMKSVIGDAPLKIKASGGIKDYETAMRFIEAGVQRIGTSNGIAIVQHKPTL
                     GNEY"
     gene            complement(2583..3185)
                     /locus_tag="PLF48_09420"
     CDS             complement(2583..3185)
                     /locus_tag="PLF48_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49588.1"
                     /translation="MMNYNFYKDTFLINYNGSKLVDSIKHFNGIDNRHFFRNVSNHLD
                     SCRYTESGKSLFYSNFAESGGQPFTYDHSNVYTCGVFNTCYQTNKDTILYNTRINQAN
                     LPGQFLTMPLSEGISLLDLNPLFLVQQSSIFLYQPHANLISNLYTSYSILVSQSPPKR
                     LHFRYEYSFDSKGRITLIYQYDDLLSTSIPFKTYALTYFD"
     gene            complement(3430..4239)
                     /locus_tag="PLF48_09425"
     CDS             complement(3430..4239)
                     /locus_tag="PLF48_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49589.1"
                     /translation="MNNCLKALCIVFIMMTISCKKEDNSGNTPVDYSKFRIKQQIHDG
                     TLQAYSDTTTYYYSGDDIQTRQNRSNNPTYFYTTNYVKVGSQYNYSTIYGLNNTHEGF
                     LKVNAMGYIDTSRVTVISSGAYNNRSKYYYNAAGYPIREINNYNTYESDIKRYYNSSN
                     DYSYQIYDFTHFTNPALSYKDSSVYEFYTDKDFLVNYDIVLPYGMHTAHLLKKKSNYR
                     LTSGGALVSTREYFYELDAIGLVKKLIVNVYNQPGNVLYSSDTTTYSYIQL"
     gene            complement(4251..>4557)
                     /locus_tag="PLF48_09430"
     CDS             complement(4251..>4557)
                     /locus_tag="PLF48_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49590.1"
                     /translation="VNSATIKGESYGTLKDFAAVMTENPDVKVKIVGHTDSDGDDAKN
                     MELSKKRAESVKSALNKEFGIDNSRMETEGKGETQPADNNNTPEGKANNRRVEFIKL"
BASE COUNT         1361 a          852 c          796 g         1548 t
ORIGIN      
        1 aattgctttg taagttttag actgttggtc gtagtattgt tgaactccgt tttctacttt
       61 ataaaaagaa gtatttcctg ctgccagcat ttctttcacc caatctgcaa cttttaatcc
      121 atctgcttcg gccaaatcaa tgcctttttg aacgcccacc atatcccaat actggaacgg
      181 accaacttcc caggcaaaac ctgcgcgcaa ggcatcgtca atggcataga gtacatcaga
      241 tatttccggc acacggttag atacataagc aaacaaaccg gctaaagaac gctttactaa
      301 ctgagcacct ttgtcgtcag ctttgataaa tgcatttact ttttctttaa gagattctgc
      361 ttttttagca gcatctaatg aagcaaattt tggtttgccg gtaggctcat attgcaacgt
      421 gtctaaacgc agtgaaaacg aatttcgttt atcgtcttta tagtaatatc cttttttaga
      481 tttgtttccg aaaaatttat tatctagtag gaattgtaca taatttggaa cggcaaatgc
      541 accggcttgt tcatcattcg ggcagttttc tttgatgccc atcataactt ttgttccggt
      601 atcattacct actaagtcag aaagtttgaa tgtacctgtt ttaggacgac caattgcagg
      661 acctgtaagg gcatccactt cttcaatagt caagcctaat tctgcagtaa gctggaatac
      721 ctttgccata gcataaacac ccactcggtt ggcaataaat gccggagtgt ctttacataa
      781 aacagtttct ttacccagaa atttgtcgcc gtaatgcatc aggaaatcga ctacttcagg
      841 agatgttctt ggtgtaggaa tgatttctaa taagcgcaga tagcgcggtg ggttgaaaaa
      901 gtgcgttccg cagaagtttt tctgaaagtc ttcacttaat ccttcttcca aaaggtgaat
      961 aggaataccg gaagtgtttg acgtaattaa tgtacccggt ttgcggaatt tctctacacg
     1021 ctgatagaca gattgtttga tatctaatcg ttctacaacc acttcgataa tccagtcaca
     1081 atctttgatt ttaggcatat cgtcatcaaa gtttccgaga gtaactctct ttgtaacttc
     1141 ggcactgtat gcctgagccg gtttggattt taatgcggca ttgaacatat ccgttacaat
     1201 gcggttgcgt gcttttgggt tgtttttttc ttcctctttc aggtcaaaag gaacgatatc
     1261 catcagcaag acttcgcagc caatgccggc gaagtgcaat gcgatacccg aacccatgac
     1321 acccgagcca agaactgcta cttttttaat tgctctgttt gccataatta taataaatta
     1381 aattttttct atagaataaa gttagccaaa agaacatcaa ctctaagaag ctagccatgg
     1441 aagtaataaa aacttaacaa ttcaatgcat gcattggaat ttaattggga gaattggtta
     1501 attagttaat tcgttgatta gttaattcgt tgattggtta attagctgat tggttaatat
     1561 tcaaactatt aaactactaa actactaaac tattaaacta tctaactact aaactatata
     1621 aattaatatt cgtttcctaa agttggttta tgttgcacga ttgcaattcc attgcttgta
     1681 ccaatgcgtt gtacgcctgc ttctataaag cgcatggcag tttcataatc tttgatgccg
     1741 ccactggctt taattttcaa gggtgcatca ccgattacgg atttcatgat ggcaatatcc
     1801 gaaagtgttg ccccgccggt gccaaaacct gtcgatgttt ttataaaatc agcaccgctt
     1861 tgcagtatga tttcagttgc tttttttatc tcattttccg ttaaaaaaca agtttctata
     1921 ataaccttca aaacatttac accaatatct gctttacata atttaatttc attcaaaata
     1981 aaatcccatt tcccattttt tgcaaaaccg atattcatca ccatatccac ttcatcagct
     2041 ccgttaataa ctgcattctt cacttcatat atttttgaaa atttaccacc tgcacctaat
     2101 ggaaatcctg caactactgc gagctttaca tcactatcct ccagtagatg attgcaagtt
     2161 tcaatccagc atccattaac acaaacggca taaaatccgt attgtttggc gtcctcacaa
     2221 agtgtaataa tatccgtttc ctgtgcagtt ggctttaaca acgtgtggtc aatataagtg
     2281 gctatattca tacagcaaag gtaggcagat tttgggagtt tgcaatttgt ttgacctgtt
     2341 cttttgtact catttaaccc gtagtgcggt ttaaaccacg tagaagtata gcatttttaa
     2401 tcataaatcg ttgtttgaag caaatcagtt taagatagtc cggctaaaac atagtattgc
     2461 ccttggcgat tcctgtattt ttctattctt tcctcaattt ttgtatgcta tgtattgtta
     2521 atcctaaaaa atctatgcat ctgagtggta aaattataaa tcaaaccgaa taagagatta
     2581 atttaatcaa aataggttaa tgcatatgtt ttgaacggga tgcttgttga taataagtca
     2641 tcatattgat aaataagcgt aatccttccc ttgctgtcaa aagaatattc atacctgaaa
     2701 tgcaatcttt tgggaggtga ttgtgaaaca agaatagagt aagaggtata caagttcgaa
     2761 attaagtttg catgaggctg atatagaaat atgctactct gctgaaccaa aaacaagggg
     2821 tttaaatcaa gtaagctaat tccttcagaa agaggcattg ttaaaaactg ccccggcaga
     2881 ttggcttggt tgattctggt attataaaga atcgtgtctt tgttggtttg ataacaggta
     2941 ttaaatacac cgcaggtata aacattagaa tggtcataag taaacggctg tccgccactt
     3001 tctgcaaaat tactgtaaaa caaggatttg ccggattctg tatatcggca tgagtctaaa
     3061 tgattggaaa catttctaaa aaaatgccga ttatctatcc cgttgaaatg ttttatagaa
     3121 tcaaccaact tactaccgtt gtaatttatc aggaacgtgt ctttgtaaaa attataattc
     3181 atcataaccc aggctttatt gtctgcatag gtaatattcg cgtaattgcg tccatctctt
     3241 gtaatgctat ttaaatttgt gccatcatat gaaaaagagt aattagctgt atcgtcatta
     3301 aataaatggt taactctttt attttgtaat taatagcagg cgcgggtgaa gtcggatcac
     3361 aactaaccat cgcgataaat gcaacaagca agtaactaat aaataaggat gttttcattc
     3421 tagtttacat cagagttgaa tataactata cgttgtcgta tcggacgaat ataaaacatt
     3481 gcccggctga ttgtacacat tgacaattag tttttttacg agtccaatgg catctaactc
     3541 ataaaaatat tctctggttg atactaatgc accaccggaa gtcaatctgt aattactttt
     3601 ctttttgagc aaatgtgcgg tatgcatgcc ataaggcaat acaatatcat aattcactaa
     3661 aaaatcttta tcggtataaa attcgtagac gctggaatct ttataagata aagccggatt
     3721 tgtaaaatgc gtgaaatcat aaatctggta ggagtaatca ttcgaactgt tgtaatagcg
     3781 cttaatatca ctttcgtagg tattataatt attaatttca cgaattggat aaccggcggc
     3841 attataataa tatttagaac gattattata ggcacctgaa gaaattaccg taacacggct
     3901 ggtgtctata tagcccattg catttacttt cagaaagcct tcatgtgtat tatttaatcc
     3961 gtaaattgta ctataattat actgactgcc cacttttaca tagttggtgg tataaaaata
     4021 tgttgggttg ttactgcggt tttgtctagt ctgtatatca tccccagaat agtagtatgt
     4081 cgtggtatca gaataagctt gaagtgtacc atcatgtatt tgttgtttta tgcgaaattt
     4141 tgaataatca acaggggtgt tccccgagtt atcctctttt ttacaagaaa tagtcatcat
     4201 tataaacaca atgcatagcg cttttaaaca attgttcatt tgtaatatat ttatagttta
     4261 ataaattcaa ctcttctgtt attggctttg ccctctggcg tattattatt atcggcaggt
     4321 tgcgtttcgc ctttgccttc tgtttccatg cggctgttgt caataccaaa ttctttattc
     4381 aaagcacttt taacagattc tgctcttttt ttagagagtt ccatgttttt agcatcatca
     4441 ccatcgctgt cagtatgtcc cactattttt actttcacat ccgggttttc tgtcattaca
     4501 gctgcgaagt cttttaatgt tccataagat tcgcctttaa tggttgcact gttcacg
//
LOCUS       DAOQII010000210         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11394_length_4537_cov_4.557117, whole genome shotgun sequence.
ACCESSION   DAOQII010000210 DAOQII010000000
VERSION     DAOQII010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11394_length_4537_cov_4.557117"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..307)
                     /locus_tag="PLF48_09435"
     CDS             complement(158..307)
                     /locus_tag="PLF48_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49591.1"
                     /translation="MLRCAPHPINPKEKKKKNGKKKEKKKATFLGGEGGLSRFFGIKI
                     LPHNE"
     gene            328..750
                     /locus_tag="PLF48_09440"
     CDS             328..750
                     /locus_tag="PLF48_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49592.1"
                     /translation="MRTLQIKSFSKDQNYQGHFFFILCKGMNSGKPLKSPCPNCFVCI
                     CQSEQEKELLYWLSFGLWHAKAFHSYLRGSVIPFLTISDVQQQLSKSLVKAVANPQSL
                     QESIQLFQKLDNLEALYEKNLQLIRDTRRAMFYRRSFL"
     gene            complement(952..1752)
                     /locus_tag="PLF48_09445"
     CDS             complement(952..1752)
                     /locus_tag="PLF48_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49593.1"
                     /translation="MAKQKGPLKYVGTIGDIRHFKIKGQEGYFAGMVGGPTDTQVKTA
                     PEFARTRENMNEFGGCAKAGKSVRVALSEVLNGMTDPQCTGRLTSIMKKINLEDGTEA
                     RGVRKVEISTQRTYLHGFGFDKNISFASVSYVPYASTHSVDRLTGTLTTYAANPANSI
                     NAPAGATHFRFINALGVVSDFAFNSSTGTYEPTSPELNELSNTTFGAYSPLSAPYAGE
                     VLDVLLPATVLTADVSVLHCIGIEFFQEVNGNYYRFSSGNCLTIDNVY"
     gene            complement(1778..2287)
                     /locus_tag="PLF48_09450"
     CDS             complement(1778..2287)
                     /locus_tag="PLF48_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNF49594.1"
                     /translation="MRRTYSKSVIIILLLITSITYAKETARVITVKDGDTYVLKNNST
                     EFTVRLKNVDAPESKQHFGFQSTLYVSKLILGSIVQYESSGTDIYNRVLADVWVGNKR
                     LDSIIIRNGWGWLYVQYSNDLVLKNCMQLAIDEKIGLWKCGTMAVCPPSLWRKYSKKD
                     KNRYCNGCR"
     gene            complement(2741..2824)
                     /locus_tag="PLF48_09455"
     tRNA            complement(2741..2824)
                     /locus_tag="PLF48_09455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2789..2791),aa:Leu,seq:taa)
     gene            complement(2866..2938)
                     /locus_tag="PLF48_09460"
     tRNA            complement(2866..2938)
                     /locus_tag="PLF48_09460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2903..2905),aa:Gly,seq:gcc)
     gene            complement(3463..4053)
                     /locus_tag="PLF48_09465"
     CDS             complement(3463..4053)
                     /locus_tag="PLF48_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF49595.1"
                     /translation="MKRLFLAFTMMASTFGVFAQETVPSAVAPDPNAPTMNFAKDAID
                     YGTIKQDADGYRTFSFTNEGKTPIVISEAHGSCGCTVPTYPKEPIMPGQKGEIKVHYD
                     THRVGPFSKSVTVNSNAKNSPVVLKISGTVEAAATDPTATEPNSIIPAPATAPAKVAP
                     APAKTATATKAATTTKKPATSTAKKSTHSHSNTKKK"
     gene            4196..>4537
                     /locus_tag="PLF48_09470"
     CDS             4196..>4537
                     /locus_tag="PLF48_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49596.1"
                     /translation="MLRAVLLISICFFSVYTSTAEVNVAVGGRAGLNISHLRKFSTPT
                     DYKKRANLGSDLAAFLRIDFNKYVGLQTEVEFIQKGQRWNRTLDSAKFTSKSVLNYIQ
                     FPILAVGRVGNE"
BASE COUNT         1321 a          934 c          838 g         1444 t
ORIGIN      
        1 ggttagttca accatccgtc tatggctgta tccttcatac ccaaaaaata aaaaaagaaa
       61 gcaaagataa gggtgctcgg gattgtcctg tcaaggttaa aatgaatttt ttgtaaaaaa
      121 atctccacct caatcagcat accaccccaa ccacttctca ttcgttatga ggtagtattt
      181 ttattccaaa aaaccttgac agtccaccct cgccccctaa aaaagtggct ttcttttttt
      241 cttttttttt gccgtttttt ttcttctttt cttttgggtt gatagggtgc ggagcgcagc
      301 ggagcatatc atttaaatgc tctcattatg cgtacactac aaatcaaatc gttcagcaaa
      361 gaccaaaatt atcaaggtca ttttttcttc atcctctgca aaggaatgaa cagcggaaag
      421 cccctgaaat ctccctgccc gaattgtttc gtctgcatct gccagtccga gcaggaaaaa
      481 gaactcctgt actggctttc tttcggatta tggcacgcaa aagccttcca cagctacctg
      541 cgaggctctg taattccatt tctcaccatc agcgatgtgc agcagcagct ttcaaaatcg
      601 ctcgttaaag ccgttgcaaa cccgcaaagc ttgcaagaat ccatccaact ctttcagaaa
      661 ctcgataacc tggaagcact ctatgagaaa aatctccaac tcatccgcga cacccgacgc
      721 gccatgtttt accgaaggtc ctttttataa ggatgggtaa ggcatatatg agccatacat
      781 aaggcatacc tattttaccc ctgattcgcc tatataaata aatacgctgt aaataaaaaa
      841 tccctgccac acgggcaggg attccaatta gtagtcaata atcaagcacc agtcgatggg
      901 ttgcgcccat cacgctttgc tctgcgatgt acatttacag gttttaccat cttagtacac
      961 gttatcaatc gttaaacaat taccggagga gaatctgtag taattcccgt tcacttcctg
     1021 aaaaaactct atgccgatac aatgcagtac agacacatcc gcagtcaaga cggtagcagg
     1081 aagcaaaaca tccaacacct cgccagcata cggagctgaa agcggactat acgccccaaa
     1141 agtggtattg ctcagctcat tcaattccgg actggttggc tcataagttc cggtagaact
     1201 gttaaaggca aaatccgaaa caacacccaa cgcattgata aaccggaaat gcgtagcacc
     1261 tgcaggcgca ttgatagaat tggcaggatt tgccgcataa gttgtcaggg tacctgtcag
     1321 tcggtcaaca gaatgcgtgg acgcatacgg cacgtatgac acgctggcga aagaaatatt
     1381 cttatcgaac ccaaagccat gcaggtaagt gcgctgcgta ctgatttcca ctttacgcac
     1441 accccttgct tctgtgccat cctccaggtt gattttcttc atgatcgaag tcaaacgacc
     1501 tgtacactgc gggtctgtca tgccattcag cacctcagac aaggcaacac gcacggattt
     1561 acccgcctta gcgcaacctc caaactcatt catgttttcc cgcgtccttg caaattcagg
     1621 agcagtcttt acttgcgtat cggtaggacc gccaaccatg ccggcgaaat atccctcttg
     1681 acccttaatc ttaaaatgtc gtatgtcacc gatagttcca acatacttca agggtccttt
     1741 ttgtttagcc ataataaata atttaattag ttaattttta cctgcaacca ttgcagtatc
     1801 tgtttttgtc tttttttgag tattttctcc aaagggatgg tggacaaaca gccatagtgc
     1861 cacatttcca taatcctatt ttttcgtcaa ttgcgagctg catacagttc ttcaagacta
     1921 aatcattgct atattgtaca tatagccacc cccatccatt tcttattata atgctgtcaa
     1981 gacgtttatt tcctacccaa acgtctgcaa gtactctatt gtatatatct gttccagacg
     2041 attcatattg tacaatactg cctaatatta gtttactcac gtatagagtg ctttggaatc
     2101 caaaatgttg tttgctttcc ggggcatcca cattctttag ccttactgta aattctgtac
     2161 tgttattctt taatacatat gtgtcaccat cttttactgt tattactcta gctgtttctt
     2221 tagcataggt aattgatgtg attaataata gaatgattat tactgattta gagtatgttc
     2281 gtctcatcag tataatttaa agaagatatc ataggtgtgc aagcaggtac ttccgatgta
     2341 tttttttctt gccgatattt ccattaattt tcttttattc caagctaaaa gttagtatat
     2401 tataagtaga ggtgttaagc tcgccactaa ctaatataaa aaagcatgta attatggata
     2461 gaatttgtat ttaccctaaa gatgttcagc tgataacagg aaagagcgaa cggtggggaa
     2521 gggaaatcat caaaaaaatt aaggagaagt ttaataaaga agatcatcaa ttagtaacta
     2581 ttgatgaatt atgcagctat ttaggaatag agccggatgt gataagaaag cttttgagat
     2641 aaaatttagg aaaaagcgag ttttgttact tatttgttac tcgtcttaat aaaaaatgac
     2701 ataataaatt gattttcaat cttttatgcc atttaaaaag tacccggggc gggactcgaa
     2761 cccgcacagc cgcgatggcc acaggatttt aagtcctgcg tgtctaccat tccaccaccc
     2821 gggcgcaatt ttaaataaaa aaaggagtcg gaaaactcct gaatgagcga aagacgagac
     2881 tcgaacccgc gaccctcacc ttggcaaggt gatgctctac caactgagct actttcgctt
     2941 gtgttttaaa gaacatgtcc gcattttgct aacggattgc aaagatagta atctttttat
     3001 ttatgtcaat aggatactta aaaataattt tcaaaattat ccacagtggt ataactctat
     3061 ttgcttttat cttagaacat tttggcatgg cgtctttctc ttgctttcgt tttgataatt
     3121 gctcaaattc agcccacttg tctttttgct tgttattgct aaaacggttc tgccttcagc
     3181 atttttcact cttaagtatc ctcaagtact tctttataat gacaaggata tgctggcatt
     3241 aaaatgtttg cttatgcgtt ttcgtatttt tcaaaatgaa acgtatcgta gattgcctca
     3301 aaataccgtt gagcaaataa aatctcaatt agatttgtag gatgtgaaat tagaagcagc
     3361 caccttttca agaacttttt agcgaatatg ttagtctttc aagataaaaa aagcgccgat
     3421 ttatcaaaga taaatcggcg ctttaaacca aaatactaag tattattttt tcttagtatt
     3481 gctatgagag tgtgtgcttt ttttagctgt tgaagttgcc ggttttttag tggtagttgc
     3541 tgctttagtg gcagttgcag ttttagcagg agccggagct acttttgccg gtgcagttgc
     3601 cggagcagga attattgaat taggttcagt tgcagttgga tcagtagcag ctgcttcaac
     3661 agtaccgctg atttttaaaa ctacaggtga gttttttgca tttgagttca ccgttacact
     3721 tttagagaac ggacctacac ggtgtgtgtc gtaatgaact ttaatttctc ctttctgtcc
     3781 cggcataatt ggttccttag ggtacgtagg cacagtacag ccacatgagc cgtgtgcttc
     3841 acttattaca attggtgttt tcccttcatt cgtaaaagaa aatgttctat atccatctgc
     3901 atcttgtttt attgttccat aatcaattgc atcttttgca aaattcattg taggtgcgtt
     3961 aggatctggt gctacagcag aaggaactgt ttcttgtgca aatacgccaa aagtacttgc
     4021 catcattgta aatgctaaaa ataatctttt catgatagta atttttttat tagtgtaaca
     4081 aatgtaatgc cttttgattt tacccatgca gatagcaact atttttaagt atagtttaac
     4141 aaatttcaat aattcttaca aaaactaaag tatatttgcc caaaatacat gtataatgtt
     4201 gcgtgctgtc ttattaattt caatttgttt tttcagcgtc tatacctcaa cggcagaagt
     4261 aaatgtcgct gtgggcggta gagccggatt aaatatttct catttacgca agttttctac
     4321 tcctacagat tataaaaaac gtgcaaattt aggctctgat ttagcagcat ttttacgcat
     4381 agattttaat aaatatgttg gcttgcaaac ggaagtggag tttattcaaa aagggcaacg
     4441 ctggaaccgc actttagatt ctgcaaaatt tacgtctaag tccgttctaa attatataca
     4501 attcccgatt ttggctgtag gaagagttgg aaatgaa
//
LOCUS       DAOQII010000211         4516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11471_length_4516_cov_4.296570, whole genome shotgun sequence.
ACCESSION   DAOQII010000211 DAOQII010000000
VERSION     DAOQII010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11471_length_4516_cov_4.296570"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLF48_09475"
     CDS             complement(<1..513)
                     /locus_tag="PLF48_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49597.1"
                     /translation="MKRIGFVIIATVLIAFACKSGKRQKNSPVTNENKRTAKLETLTK
                     NGITLTELYSPEQFENATLQQEINSSYEDLDTNTIALKFTTTNYEFGVPTDTTGLKNC
                     ANSAKGQHVHTLVDNGPYNALYTPMAKVKAGNDGQHILLSFLSRSYHESIKNKNAYVL
                     TSAITGKSRIY"
     gene            complement(510..2006)
                     /locus_tag="PLF48_09480"
     CDS             complement(510..2006)
                     /locus_tag="PLF48_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49598.1"
                     /translation="MIDIQLKYHLHEKWFTKNQLSVIGTAFIGEKLLHDKALYDFFDG
                     ELNEAVFKQKLNQLSGHFAVVIKKENEVYFAVDYLRTYPLFYFISNKEVLICDNIVQY
                     KIWNENTSDYFSKLFCTPENETLLKNWKQLQAGAYGIFQTENGNTVFKQDFYYKHQAV
                     HTQAFDKNLLKQLEDKLIYQIKTIAQDKTILLPLSGGYDSRYLLSLLYQHNIKSVVCF
                     TYGKKDSYEVVFARNSAEKLKYPWHFIDYDDNLLSLFLGEEWNSYAELNHHYTSLPHE
                     QDFFALHYLKSKNLLPENAIVMNGFCQDLHGGSIFESGRRFDATSYIQSKYKVVAPAS
                     VTTSKNDYQAYQDWFIRNRVSKFIINAVHVYAYFGLSFYLPFWDKTWIQFWYNLPIKE
                     RIGQQVYNDYIFSDYFKPLFVDFKKPLFDTTLALHSLKQTAKKILPKKIVEAIQNQNA
                     NRDEYDVNNTRFLYEFIYKKLKNTPPQKNYKINEIHALFLLESLKNEL"
     gene            complement(1999..3189)
                     /locus_tag="PLF48_09485"
     CDS             complement(1999..3189)
                     /locus_tag="PLF48_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF49599.1"
                     /translation="MLKGKFIKQVSVLFSGLLMVQIINFIFSLLLPKYVTPPDFALFG
                     IFTAVVFILAEIVNAKLDVAVMLGTDDKEAERLVTAAHTFAAGFVLLLIFISLFLVFY
                     VDKIYVLLPFAVAAYGIHQPVLVRFNQQEEYKKINTFRFIQVSTTALMTLTLAILKIK
                     YALIIGFCIGLGIATIYTMYFMPVKLNVQTLIFCLKKYKQFPTYGTWSSLLNNLSKNS
                     IPILLKRFFSANEVGYYAYATRLLNAPTGMYSSAISQVYFKNAVSLNPAELKQHTYQL
                     IRMSVLLGIIPSILILFFGKSIFLYLFSPEWANAGVVAQYLILWYFIGIITGPISVLL
                     DIKYKLKAELFYNLLLLLLRVMAILLGAYLHNFLLAILTLSLVGFVMNLILLNYLLYL
                     VNHD"
     gene            complement(3391..>4516)
                     /locus_tag="PLF48_09490"
     CDS             complement(3391..>4516)
                     /locus_tag="PLF48_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49600.1"
                     /translation="DKYTGYFTSNRPDGHGLDDIYSFTDNGIELEGVVVDSLTGKPIC
                     NSSVQMTNDGITLGTKKTKCDGYFEFSVLRDMDYCFPAKAEGYYPNNSICATTKGVKP
                     GEKVFVRIPLLPERPASLTVQVIDKQTKKPIPHAGILLSANCDGTIQNDTANENGKKC
                     FKVKCDCDYVAVGNAEGYLPNQSFYTTKDKCDKLKECDNPNGEVVIVELDKFRQPEQP
                     KEPEVVLPKGYTVDSAGYIELKDIYYDFDKWYIRKESETELYKLLAFLIFNPDAIVEI
                     ASHTDSRAPFDYNIKLSQRRAQSVVNWLAAKGIDRDRMKPVGYGETRLRNKCADDVPC
                     TEEEHQRNRRTEFKVIGKKINLSSEEKKGVKVDPCLKCPF"
BASE COUNT         1439 a          805 c          773 g         1499 t
ORIGIN      
        1 atatattctg gatttacctg taattgctga ggtcaagaca taggcatttt tatttttaat
       61 actttcgtga taggaacgcg ataaaaatga caataggata tgctgtccat cattaccggc
      121 tttcactttt gccataggcg tatacaatgc attataagga ccattgtcaa caagcgtatg
      181 cacatgctgt ccctttgctg aattagcaca gtttttcagt cctgtggtgt cggttggcac
      241 accaaactca tagtttgtcg ttgtaaattt caatgcgatg gtgttcgtat ccaaatcttc
      301 ataagatgaa ttgatttctt gttgcagcgt tgcattttca aattgttcag gagaatataa
      361 ttctgttagc gtaatgccgt ttttagtaag cgtttccaat tttgcagtgc gtttattttc
      421 attagttaca ggactgtttt tttggcgttt tcccgattta caagcaaatg caattaatac
      481 agtagcgatg atgacaaatc ctattctttt cataattcat tttttaaaga ctctaacaaa
      541 aataaggcat gaatttcatt tattttataa ttcttttgag ggggggtatt ttttagtttt
      601 ttatatataa attcatataa aaatcgtgta ttgtttacat catactcatc tctatttgca
      661 ttttgatttt gtatagcttc gacaattttt tttggtaata ttttttttgc agtttgtttt
      721 aggctatgca aggccaaggt cgtgtcaaat aagggctttt tgaaatcaac aaaaagtggt
      781 ttaaaataat cagaaaatat ataatcatta tatacctgct gaccgatgcg ttcttttatc
      841 ggcaaattat accaaaattg tatccatgtt ttatcccaaa aaggcagata aaatgacaat
      901 ccgaaatatg cataaacatg tacagcgttt ataatgaatt ttgaaacacg atttctgata
      961 aaccaatcct gataagcttg gtaatcattt ttagaagttg taactgaagc cggagctact
     1021 accttgtatt tcgattgaat ataagaagtt gcatcaaatc ttcttccgga ttcaaatata
     1081 ctgcccccat gcaaatcctg acaaaagcca ttcatgacaa ttgcattttc cggcagtaag
     1141 ttttttgatt ttaaatagtg taaggcaaaa aaatcttgtt cgtgtggtaa ggaagtataa
     1201 tgatgattta gttcggcata actattccac tcctctccta aaaacaaact caacaaatta
     1261 tcgtcatagt caataaaatg ccaggggtat tttaattttt ctgcagagtt acgggcaaac
     1321 acaacttcat agctgtcttt tttgccataa gtaaaacaga caacagattt aatattatgc
     1381 tgatatagta aagacaataa ataacgtgaa tcatagccac ccgaaagcgg caataagata
     1441 gtcttatctt gtgctatggt tttgatttga taaattagtt tgtcttccag ttgcttcaat
     1501 aagtttttgt caaaagcttg agtgtgaact gcttgatgtt tataataaaa atcttgttta
     1561 aaaactgtat ttccattttc agtttgaaat attccataag ctcctgcttg aagctgtttc
     1621 cagtttttaa gtaatgtttc attttccgga gtgcaaaaca gtttagaaaa ataatcactc
     1681 gtattttcat tccatatttt atactgaaca atgttatcgc aaatcaatac ttctttatta
     1741 gaaataaaat aaaacaacgg ataagtacga agataatcaa cggcaaaata aacttcattt
     1801 tcttttttga tcacaacagc aaaatgcccg cttagctgat tgagtttttg tttaaagacg
     1861 gcttcgttta attccccatc aaaaaagtca taaagtgctt tatcatgtaa taatttttct
     1921 cctataaatg ctgttccaat tacagaaagt tgattttttg taaaccattt ttcatgcaga
     1981 tgatatttaa gctgaatatc aatcatgatt aacaagatac aataagtaat tcaacaagat
     2041 taaattcata acaaaaccaa ccagagataa agttaaaata gccagcaaaa aattgtgtag
     2101 gtatgcgcct agtaaaattg ccatcacacg caataatagc aataagagat tataaaagag
     2161 ctctgctttg agcttgtact ttatgtctaa caagacacta atcgggccgg taataatgcc
     2221 aataaaatac cataagataa ggtattgagc tacgactccg gcatttgccc attcaggact
     2281 aaataaatat aaaaagatac ttttaccaaa aaataaaata agaatactgg gtattattcc
     2341 cagcagtacg gacatacgaa tgagctgata tgtatgttgt tttaattcag caggattgag
     2401 cgaaactgca tttttgaaat aaacctgact aatagcagaa ctgtacatgc ccgttggagc
     2461 atttaataat ctggtggcat aagcataata gccgacttca ttagccgaaa aaaatcgttt
     2521 gagtaaaatg ggaatgctgt ttttggacaa gttgtttagt aacgaagacc acgtaccata
     2581 agtgggaaat tgtttgtatt tttttaaaca gaaaatcagg gtttgtacat ttaacttaac
     2641 gggcataaaa tacatagtgt aaatcgtagc tatacctaat cctatacaaa aaccaataat
     2701 cagtgcatat tttattttca atatggccaa ggttaatgtc attaatgctg tagtacttac
     2761 ctgaataaaa cggaaggtat taattttttt atactcttct tgctgattaa atcgcactaa
     2821 aacaggttga tgaatgccgt aagcagcgac tgcaaaaggt agcaagacat atattttatc
     2881 gacgtaaaaa accaggaaca gactgatgaa aatgagtagc aagacaaacc ccgctgcaaa
     2941 tgtatgtgct gcagttacca gtcgttcagc ttctttgtca tcagttccta acatcactgc
     3001 aacatcaagt ttggcattta caatctcagc taagataaat acgacagccg taaatattcc
     3061 gaacaaagca aaatctggtg gtgtaacgta tttgggcagc agtaaggaga aaataaaatt
     3121 gatgatttgc accatcagca aaccggaaaa caaaacggaa acttgtttaa taaactttcc
     3181 cttcagcatt gctacaagat acggatttta acctatcgga tgacgattcg ataataaaca
     3241 agttttgtta taagttgtat taaattaatg aaatagcatt tattacaaga cttcactgta
     3301 taaataaaga ctgcctgttc caatatgaac aggcagtttt tcaacggggg ttgtcatctt
     3361 cagtttttca atatatagct atttggtttt ttaaaacgga catttaagac atggatcaac
     3421 ttttacccct tttttctctt ctgacgataa gttgattttc ttgcctatta ctttaaattc
     3481 tgttctgcgg ttgcgttggt gttcttcttc ggtacatggc acgtcgtctg cacatttatt
     3541 gcgcaatcgg gtttcaccat agcctacagg tttcatgcgg tcgcggtcta taccttttgc
     3601 tgccagccag ttcacaacag actgcgctct gcgttgcgat aatttgatat tgtaatcaaa
     3661 tggtgcacgt gagtcggtgt gtgaagcaat ctctacaata gcatccggat tgaaaattaa
     3721 aaatgccaaa agtttataca attccgtttc agactctttg cggatatacc atttgtcaaa
     3781 atcataataa atatctttca actcaatata acctgccgag tcaacggtat aacctttagg
     3841 caagactacc tccggctctt taggttgttc cggttgacgg aatttgtcta attctactat
     3901 caccacttcc ccattcgggt tgtcacattc tttcagctta tcgcatttat ctttcgtcgt
     3961 ataaaaagat tgattgggca aatagccttc agcgttgcca acagccacat aatcgcaatc
     4021 gcatttcact ttaaagcatt ttttaccatt ttcattggcg gtatcattct gtatggttcc
     4081 atcgcaattt gccgataata aaataccagc atgcggaatc ggctttttag tttgtttatc
     4141 tataacttgt acggtcaagg aagcaggtcg ttcaggcagc aacggaattc tcacaaatac
     4201 tttctctccc ggttttacac cttttgtagt agcgcaaatg gaattgtttg ggtaataacc
     4261 ttcggcttta gccgggaaac aatagtccat atctcgcaaa acgctgaatt caaaatagcc
     4321 gtcgcatttt gttttcttcg tccccaaggt aataccatca ttagtcatct gtaccgagct
     4381 gttacaaatt ggttttccgg tgagggaatc tactactact ccttccagtt caatgccatt
     4441 atccgtaaat gaataaatat catccaatcc atgaccatca gggcgattgg atgtaaaata
     4501 accggtgtat ttatca
//
LOCUS       DAOQII010000212         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11523_length_4504_cov_4.625534, whole genome shotgun sequence.
ACCESSION   DAOQII010000212 DAOQII010000000
VERSION     DAOQII010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_4504_cov_4.625534"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PLF48_09495"
     CDS             complement(<1..1260)
                     /locus_tag="PLF48_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49601.1"
                     /translation="MSLSTATYGLLIIYVIITLTIVIRGALKIKTMSDYAVGNIVFKP
                     WMVGLSLAASTTSAATFIINPGYIALYGWSAFISFAVVLPIGVFLSLYFMTKRFREHG
                     VTIKALTLSQWIGTRYNSKAYAVFFAFLSLLLITFIVLICVGLASILSKALNLDIIYV
                     VVGIIVFVFGYMMFGGANSMVYTNTIQASIKLIVAFIMLGSGIHFFKNGFNETLSSID
                     TNLVSVVNPSSPMFRDFFEIFICQFIVGIAIVCQPHIITKSLLLKKDEDVNKYLTIGI
                     IVLAIFFSVVFTGFYARLSFPDLMVNGTKLKMDMIIPTYVVQTFSGNIGVFVGVFVIL
                     GLMSSGIATLEGLIQSLSTTISSDIIKPLLNIDESETNGDKKLFYINRIIIVCLALIS
                     MYFSIQQIQHPKLSVSLFALVGVYGFFA"
     gene            complement(1263..2252)
                     /locus_tag="PLF48_09500"
     CDS             complement(1263..2252)
                     /locus_tag="PLF48_09500"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNF49602.1"
                     /translation="MRKAVITGSGAYVPEKIVPNSFFNEVLQEDVDTWLRENVHIYNR
                     RWCGENESVVDLCLSAAKNAIEDAAITADDIQLIIVATDTPEYISPSTASKLQHLLQA
                     NHTGAFDINTACAGFVTALDMASKYIIADKQYKNILVIGAYAMSKYLNIEDKKTATLF
                     ADGAGAVILSAQHDTDSGYIAGDLMTQGQYCDWMGIYAGGTFKPLSQEVLDKKEHLLQ
                     FVKKFPKELNPTMWTSMIQKLCKENKVEVNEVKGFFITQININSIWETMDRLGVDRKL
                     APTVMHEFGYTGSAAIPIAFHIARKSGKVKQGDYVVFVGSGGGLSFASVLLKL"
     gene            complement(2249..2992)
                     /gene="fabG"
                     /locus_tag="PLF48_09505"
     CDS             complement(2249..2992)
                     /gene="fabG"
                     /locus_tag="PLF48_09505"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNF49603.1"
                     /translation="MQQLQNKTAIITGAARGIGRRAAEVFAANGANVVIWDVNEEAGM
                     NVEEQIKAQGFTAYFQKVNTVDVNEVIAATAQVSEKFGAIDILINNAGITRDATLQKM
                     SPEQWQQVIDVNLTGVFNCTKAVSPYMIQQQSGRIINTSSVVALYGNFGQSNYVATKA
                     GLIGLTKVWARELGKYNITVNAVAPGFIGTEMIQTVPEKVLETMKDKSALKRIGEPED
                     IANAYLFLASDAAKFITAHTLSVDGGLTI"
     gene            3148..>4504
                     /locus_tag="PLF48_09510"
     CDS             3148..>4504
                     /locus_tag="PLF48_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49604.1"
                     /translation="MKIKFQQLTTYIEALLPHEIHLLVNLADKKDKLNYALIKKIEGY
                     VLKKQPLNFDTAIDKRRYANLKKWMQQRLLEYNVDYKYEYLLHIEELIYTDSISVDEE
                     RWIFTAISHNKYNTFNFIKWYETIEKFMQYLLIRLRYDDYNKVEHFIRTHQKRYNKCV
                     STRNKISAASIDIVHHYMGKLKNSNQWEDFLIDVMYDVEVEGYLRYMALVRLTFMYSN
                     YNNFKNLLEHYTYFDKLFSTQQLFFSKRFLLNFYANRSLILIRNKAYKQAEYYARLSV
                     KIPLNDHLLYLNNLSSILLKQHRITEALQLMNGALPIMHKTHNFHNKFSFISFFVKCL
                     IAEKEYKKAAQFAESILAVYGKEIIRYRWHLFFTVYVESLFMQQKWQQVLTIIKKYKL
                     LILDKQQKMRAGYLPWIPIYYLTAAYMEGNLSKEKFTAEYHTWRKVYKKQNKLHALFD
                     QV"
BASE COUNT         1486 a          880 c          738 g         1400 t
ORIGIN      
        1 tgcaaaaaaa ccatatacac ctaccaacgc aaataagcta acgcttaatt taggatgctg
       61 aatttgctgg atggagaaat acatagaaat gagtgccaag cagacaatga ttattctatt
      121 aatataaaac aattttttat ccccattcgt ttccgattca tcaatattta ataagggttt
      181 aataatatcg gagctgattg ttgtagatag agattgaatt aaaccttcca aagtagcaat
      241 acctgaagac atcaacccta ggataacaaa aacgcctaca aacacgccga tatttccgct
      301 aaatgtttgt accacatagg taggaataat catatccatt tttagtttag tgccatttac
      361 catcaaatct ggaaatgaca gacgtgcata aaaaccggtg aaaacgacac taaagaaaat
      421 tgctaacacg ataattccta ttgtcagata tttatttaca tcttcgtctt ttttcaacaa
      481 taatgatttg gtgattatat gcggctggca aacgatggca atacccacta taaactgaca
      541 gataaatatc tcaaagaaat ctctaaacat gggactgctg ggattcacta cggataccag
      601 atttgtatct attgaagaca atgtttcatt aaaaccgttt ttaaaaaaat gaatacctga
      661 acccagcatg atgaatgcaa cgattaactt aatggatgct tgtatggtat tagtatacac
      721 cattgaattg gcaccgccaa acatcatata tccaaataca aaaacaatta ttcccaccac
      781 tacatagata atatctaagt tgagtgcttt ggataaaata gaagccaaac ctacacaaat
      841 caaaacaatg aacgtaatta acaacagcga taagaaagca aagaaaacag catacgcttt
      901 gctgttatag cgtgtgccta tccattgtga gagcgtcagt gcttttatcg taacgccatg
      961 ctcacgaaat cgcttggtca taaaatacaa agacaagaat acgccaattg gcaacacaac
     1021 tgcaaatgat ataaatgcac tccatccgta aagtgctata taacccggat taataataaa
     1081 agtagcagcg ctggtagtag atgctgccaa agacaagcct accatccagg gtttaaaaac
     1141 aatattacca acagcgtaat cactcatggt ttttattttc aaagcaccac gtattacgat
     1201 ggtcagcgtg atgatgacat agataatcaa taatccgtat gttgccgtgg ataatgacat
     1261 gtctataatt ttaataatac acttgcaaat gataatcctc cgcctgaacc cacaaaaact
     1321 acataatccc cttgtttgac ttttccgctt tttcttgcaa tatgaaaagc tatcggaata
     1381 gccgctgagc ctgtatatcc aaactcatgc atcactgtgg gtgcaagttt acggtcaacc
     1441 cctagcctgt ccatcgtttc ccaaatgctg tttatattaa tttgtgtaat gaaaaaacct
     1501 tttacctcat ttacttcaac tttattttct ttgcacaact tctgtatcat gctcgtccac
     1561 atggtggggt tgagttcttt cgggaatttc tttacaaatt gcaacaagtg ttctttttta
     1621 tccaacacct cctgagaaag cggcttaaaa gtgcctcctg catagatacc catccagtcg
     1681 caatattgtc cttgagtcat caaatctcct gcaatatatc cgctgtcagt atcatgctga
     1741 gcagaaagaa taaccgcacc ggctccgtct gcaaataaag ttgctgtttt tttatcttcg
     1801 atatttaagt acttactcat agcataagca ccaataacca gtatgttttt atactgctta
     1861 tcggcaataa tgtatttaga tgccatatca agcgcagtaa caaagccggc acacgccgta
     1921 ttgatatcaa atgctccagt atggttagcc tgaagtaaat gctgaagctt agatgcagta
     1981 gatggcgaaa tatattcggg cgtatctgtt gccacaataa taagttgaat atcatctgcc
     2041 gtgatagctg catcttcaat ggcatttttt gcagcactta agcacaaatc cactacggat
     2101 tcattttcgc cacaccatct tctgttataa atatgtacat tttcgcgaag ccaagtatcc
     2161 acgtcttctt gcaatacttc attgaaaaaa ctgtttggaa caatcttttc aggcacataa
     2221 gcaccgctac ctgttattac tgcttttctc atatcgtcaa tcctccatct acacttaagg
     2281 tatgtgccgt gataaattta gcggcatcgc tcgccaagaa caaataggca ttggcaatat
     2341 cttcaggttc tccaattctt ttaagcgcac ttttatcttt catggtttcc agcacttttt
     2401 caggaactgt ttgtatcatt tcggtaccaa taaaaccggg tgctactgcg tttacagtga
     2461 tattatactt acccaattcc ctagcccata cttttgtcaa accgattaat ccggctttgg
     2521 tagccacata attagactga ccaaaattgc catataatgc cactacggaa gatgtattga
     2581 tgattctgcc agattgttgc tgaatcatat aaggcgaaac ggccttagta caattaaaca
     2641 ctccggtcag gttgacatca ataacctgtt gccattgctc cgggctcatt ttttgcaaag
     2701 ttgcatcgcg ggtaattcct gcattattga ttaaaatatc tatagcacca aatttttcag
     2761 aaacctgtgc tgtggcggct attacttcat ttacatcaac tgtattcact ttttgaaaat
     2821 aggcagtaaa gccttgtgct ttgatttgtt cttcaacatt cattcctgct tcttcattaa
     2881 catcccagat gaccacgttc gctccatttg ctgcaaatac ttctgccgct cttcttccaa
     2941 ttcctctcgc tgcacctgtg ataatggcag ttttgttttg taattgttgc atgtatttca
     3001 attatactat aaaattaacc tgcgtaattt taatctaata tttagtttat gattttttta
     3061 ctattgactt aaaatgtaaa ttatgcataa atctgtaaaa atagtagtat tttatggcta
     3121 attttagtaa ttaatttaat attgactttg aaaataaaat tccaacaact aacaacctat
     3181 attgaagctt tgttgcccca tgaaatacat ctgcttgtca acctggccga caaaaaagat
     3241 aaactcaatt atgctttaat caaaaaaatt gaaggatatg ttttgaagaa gcaaccctta
     3301 aactttgata ctgcaattga caaacgacga tatgctaatt taaaaaaatg gatgcagcag
     3361 cgtttgctcg aatacaatgt cgattacaag tacgaatatt tgctacatat tgaagagctt
     3421 atttatacag acagtatttc tgtagatgag gaaagatgga tatttactgc aataagtcat
     3481 aacaagtata atacatttaa ttttatcaaa tggtacgaaa ctattgagaa gtttatgcaa
     3541 tatctgctta tccgactgcg atatgatgac tataataagg ttgaacattt tatacgaacc
     3601 caccaaaaga ggtataacaa atgtgtatca acccgaaata aaatatcagc tgccagtatt
     3661 gacattgtgc atcattatat gggaaaattg aaaaactcta atcaatggga agactttttg
     3721 attgatgtca tgtatgatgt agaggtggaa ggatatttgc ggtatatggc attggtgagg
     3781 ctcaccttta tgtattccaa ttacaataat tttaaaaatc tgcttgagca ctatacttat
     3841 ttcgacaaat tattttcaac gcaacaactg tttttttcta agagattctt attgaatttt
     3901 tatgccaacc ggtctctcat tttaatacga aacaaagcat acaaacaagc agagtactat
     3961 gctcgtcttt cggtaaaaat tccattaaat gatcatctct tatacttgaa taatttatcg
     4021 tccatcttac tgaaacaaca caggataacg gaagcattac aattgatgaa tggcgcattg
     4081 cccattatgc ataaaaccca taatttccac aataagtttt cgttcatctc attttttgta
     4141 aaatgtctca tagctgagaa agaatataag aaggcagccc aatttgcaga aagcatactc
     4201 gctgtatatg ggaaagagat aatccggtac agatggcatt tattttttac agtttatgtg
     4261 gaatcgttgt ttatgcagca aaaatggcaa caggtgttga ctattataaa aaaatacaaa
     4321 ctactcatct tagataaaca acagaaaatg agagcgggat acctcccctg gattcccatc
     4381 tattatctta cggctgccta tatggaagga aatttgagta aagaaaaatt cacggcggaa
     4441 tatcatacct ggcggaaagt gtataaaaaa caaaataaac tgcatgcttt attcgaccaa
     4501 gttg
//
LOCUS       DAOQII010000213         4485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11611_length_4485_cov_4.739503, whole genome shotgun sequence.
ACCESSION   DAOQII010000213 DAOQII010000000
VERSION     DAOQII010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11611_length_4485_cov_4.739503"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLF48_09515"
     CDS             <1..459
                     /locus_tag="PLF48_09515"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNF49605.1"
                     /translation="INAGKQTVTILPGGSFFDSATSFAMIRGGHIDLTILGAMEVSED
                     GDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLPKCTLPITGLKCV
                     KKIVTELAVLDVTPDGFKLLERAPGISIEEIKNATAGKLIIEGDIPEMQL"
     gene            complement(515..1054)
                     /locus_tag="PLF48_09520"
     CDS             complement(515..1054)
                     /locus_tag="PLF48_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNF49606.1"
                     /translation="MKTKKLTKTFLVLLVALIAVTSCVGIPDGVTAVQNFQSEKYLGQ
                     WYEIARFDYKYEKNMDNTTAQYSLNEDGTIKVENKGYDYVKKEWKLATGKAKFVGSDK
                     EARLKVSFFGPFYAGYNVVAIDDAYQNALVYGSSKKYIWVLSRNKTLDETTKAKFIEK
                     AKKDGYDISKLIWVAHDKD"
     gene            complement(1245..1703)
                     /locus_tag="PLF48_09525"
     CDS             complement(1245..1703)
                     /locus_tag="PLF48_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNF49607.1"
                     /translation="MHTIDDIDLKILSILMKDARKPYTEVAKELFVSAGTVHVRMRKL
                     EEMGLVKSYNVQLDHQKLGYDITAFLGIYLNKSSQYDVVAKQLAAIPEIVDVHYTTGI
                     YSMFVKLICRDTEHLRKVLMEKIQKIEEITRTETFISLQESISRPLKLDE"
     assembly_gap    1855..1954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2043..2534)
                     /locus_tag="PLF48_09530"
     CDS             complement(2043..2534)
                     /locus_tag="PLF48_09530"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNF49608.1"
                     /translation="MDELFCHSYITSPIGTICIGANNHGIEYISFQDKNQEKADKENV
                     ITKEAARQLQAYFNKELKQFSLPLNMHGTPFQQKVWTSLHQIAYGKTISYLDLAKQLG
                     DAKCIRAAGTANGKNPFAIVVPCHRVIGKNGSLVGYAGGLHRKKWLLDFEQNTKLLFK
                     DEF"
     gene            complement(2537..3469)
                     /locus_tag="PLF48_09535"
     CDS             complement(2537..3469)
                     /locus_tag="PLF48_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF49609.1"
                     /translation="MESKKKLLVTGAAGALAKKVIDKLKIHHKIVAVDFRQKVDLGIP
                     VETYKVDFNKRIFEDIFREHQFEGIIHLGRISAHEMNRFSRYNANVMGTRKLFDLALK
                     YNVPKVLVLSSYFVYGASPYNPSLLDESTPLKASELTMDLVDSVELENLSNIYLYKYP
                     QLNITILRPCNIAGPGVRNTFSLLLSEKRAPVLWGFSPLMQFIHIDDMRDAIELAFEK
                     NKPGVYNVAPADYIAFQDAVKAAGCKTIQIPSVPPQLTESIARFLKWKTFPPYLINYF
                     KYPVILDGSSFDKTFGFKPKKTLSDIFNYYKSLK"
     gene            complement(3477..4337)
                     /locus_tag="PLF48_09540"
     CDS             complement(3477..4337)
                     /locus_tag="PLF48_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF49610.1"
                     /translation="MAKKKKNIIEKIIDTSKVDEQVRVFPNKVGSYGFDAWGFNMKDM
                     KNSMRFAKFLYEQFYKVEAFGLENIPREGRCLIIANHSGQLPFDGMLIGYAMLSNPNG
                     PRAPKAMMERFLPTVPFIGNWLNSMGAVVGDMLNAERMLENEEAIIVFPEGVRGSNKV
                     YKDKYKLQRFGNGFMHLAMKHKTPIIPVSVVGMEETIRSYADIKPLAKLLGMPVAPLV
                     IPWIFPSKVVLHFGTPMYFDNDVHKEHDIKYRVDLVKDEINNLMQSGLTHRKQAAVQQ
                     KISSKEKTKS"
BASE COUNT         1291 a          811 c          796 g         1487 t
ORIGIN      
        1 ataaacgccg gaaaacaaac cgtaacgatt ttgccgggcg gctctttttt tgattcggct
       61 accagctttg ccatgattcg cggaggtcat attgatttaa ctattttagg agcaatggaa
      121 gtaagtgaag atggagatat cgctaattgg aaaatacccg gaaaaatggt aaaaggtatg
      181 ggtggggcga tggatttagt agcttcggca aaaaatataa ttgtggcgat gcaacatgtg
      241 aacaaggcag gtgaatctaa gcttttacct aaatgtacac taccgattac cggattaaaa
      301 tgtgtaaaga aaatagttac agaactggca gtattggatg taacaccgga cggatttaaa
      361 ctgttagaac gcgccccggg aatcagtata gaagaaataa agaatgccac agccggtaaa
      421 ttaatcattg aaggggatat accggagatg cagctttaac tgcatctccg tggatatatt
      481 ctagatgtac ttttttctaa aatgtgagat ttgattaatc tttgtcatga gcaacccata
      541 tgagttttga aatatcatag ccatcttttt ttgccttttc tataaacttt gcttttgtag
      601 tttcatccaa agttttattt ctggacaaga cccaaatgta ttttttactg ctgccataaa
      661 caagtgcatt ttggtaggcg tcgtcaatag ccacaacatt atatcctgca tagaatggac
      721 caaagaatga aactttaagt ctggcttctt tgtcgctgcc tacaaatttt gctttgcctg
      781 tagctaattt ccattctttc tttacatagt catatccttt attttcaact tttattgtgc
      841 cgtcttcatt aagactatac tgagcggtgg tgttatccat gtttttttca tatttgtaat
      901 caaagcgagc tatttcatac cactgaccaa ggtatttctc actttggaag ttttgaacag
      961 ctgttacgcc atcaggaatg cctacacacg atgttacggc aattaaggct acgagaagca
     1021 ctaaaaatgt ttttgttaat ttttttgttt tcatgatttg taaattttat aatgcaaaag
     1081 tagcatcatc ttctgctatg cggttacaag attattttca attgttacaa aatgaacatc
     1141 aatactattt tgccaaaatt aaatgtaaga gtaagcactt aaacttagga tttgatactg
     1201 attgcttacc tgtgcgtatt aaactaattt aatataaact attcttattc atccagcttc
     1261 agcggtcggc tgatactttc ttgcaacgat ataaaggttt ccgttctggt aatttcttct
     1321 attttctgaa ttttttccat cagcactttt cgcaaatgtt cagtatctct gcaaatcagc
     1381 ttgacaaaca tcgaataaat tccggtagta taatgcacat cgacaatttc cggaatagcg
     1441 gctaattgtt tggcaacgac atcatattgt gaacttttat tgagataaat tcccagaaat
     1501 gccgtgatgt cgtatcccag tttttgatgg tcgagttgca cattatacga ttttaccaga
     1561 cccatttctt ccaattttcg catacgcaca tgcactgttc cggctgaaac aaaaagctct
     1621 tttgctactt ctgtataggg tttgcgcgca tctttcatga gtatagaaag aatctttaaa
     1681 tcaatatcgt caatagtatg catgatttat ttagttggca gttgacaaat tttaagttgg
     1741 caaaatgaaa ttaatcataa caatttattt ttcttttaag gcaagtgtat tagttattgg
     1801 tcattggtga ttagtcattg gttattggca attagtaaat agtgaatgtg ttttnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcatt ggtgattagt cattggttat
     1981 tggcaattag taaatagtga atgtgtttta ggtaagttat tgattcataa tttaaatttt
     2041 tttcaaaatt catccttaaa cagaagtttc gtgttttgtt caaaatcaag cagccatttt
     2101 tttcgatgca aacctcccgc atagccaact aaactcccgt tttttccaat tactctgtgg
     2161 caaggcacaa caatggcaaa tggatttttg ccgtttgctg ttccggcagc acgtatgcat
     2221 ttagcatcgc ccagttgctt tgccaaatct aaatatgaaa tagttttccc atacgcaatc
     2281 tgatgtaatg atgtccaaac cttttgctga aaaggcgtgc catgcatatt gagtggcaac
     2341 gaaaactgtt taagctcttt attaaaataa gcctgtaatt gtcttgccgc ttctttcgta
     2401 atgacatttt ctttgtcagc tttttcctga tttttatcct gaaaagaaat atactcgatg
     2461 ccatgattgt ttgcgccaat gcatatagta ccgattggag atgtaatata agaatgacaa
     2521 aataattcgt ccacttttat tttaaacttt tgtagtagtt gaaaatatca ctgagtgtct
     2581 ttttaggttt gaatccgaaa gttttatcaa acgaacttcc atctaaaatt acaggatatt
     2641 taaaatagtt tatgagatag ggcgggaagg ttttccattt caagaaacgg gcaatactct
     2701 cagtaagttg cggaggaaca gatggaatct gtatcgtttt acatcccgca gcttttacag
     2761 catcttgaaa agcaatatag tctgccgggg ctacgttgta gactcccggt ttgttttttt
     2821 caaacgccag ttcaatagca tcgcgcatat catcaatatg aataaattgc atcagcggtg
     2881 aaaaccccca taagacaggt gctcgttttt cagaaagtaa taaagagaaa gtatttctga
     2941 caccgggtcc tgctatatta catggacgca aaatagtaat attgagttgt gggtatttat
     3001 ataaataaat gtttgacaaa ttttccagct ctacggaatc tactaagtcc atggtaagtt
     3061 cagaagcttt tagcggagta ctttcatcca gcaaggaagg attatatggc gaagcaccat
     3121 aaacaaaata ggaggagagt accagcactt ttggaacatt atattttaat gccagatcaa
     3181 acaatttccg cgtgcccatt acattagcat tataacgaga aaaacggttc atctcatgtg
     3241 ccgaaatacg acctaaatgg atgatgcctt caaactgatg ttcacggaat atatcttcaa
     3301 aaatgcgctt gttaaagtct accttatatg tttctaccgg aatacccaaa tccacttttt
     3361 ggcgaaaatc tacggctact atcttatgat gaatttttag cttgtcgatt actttttttg
     3421 ccagcgctcc tgcagcacct gttactaata atttcttttt tgattccata ctgtgcttat
     3481 gatttagttt tttctttgct ggatattttt tgttgaactg ctgcctgttt gcgatgagta
     3541 agaccacttt gcattaagtt gttgatttcg tcttttacaa gatcgactct gtatttaata
     3601 tcatgttcct tatgtacatc attgtcaaaa tacatgggag taccaaaatg caaaactact
     3661 tttgacggaa atatccacgg tatgaccaag ggcgcaacag gcattcccag tagcttagca
     3721 agcggtttaa tatcggcgta agaacgtatg gtttcttcca tgccgactac gcttaccgga
     3781 attatcggtg ttttgtgctt cattgccaaa tgcataaatc catttccaaa acgctgtaat
     3841 ttgtatttgt ctttatatac tttattagaa ccccttacac cttcgggaaa tacaatgatt
     3901 gcttcttcat tttccagcat ccgctctgca ttaagcatat cccctacaac agctcccatc
     3961 gaattgagcc agttgccaat aaatggtacg gtgggcaaga aacgttccat cattgcttta
     4021 ggagctctcg ggccgtttgg attactcagc atggcatagc caataagcat gccgtcaaac
     4081 ggcaattgcc cgctgtggtt ggctattatc agacagcgtc cttctcttgg aatattttca
     4141 agtccgaagg cttcaacttt atagaactgc tcatacagga attttgcaaa gcgcatgctg
     4201 tttttcatgt ctttcatgtt gaatccccag gcatcaaaac cataagaacc tactttattt
     4261 ggaaaaacgc gaacttgttc atctactttg gacgtgtcaa taattttttc aataatattt
     4321 tttttctttt ttgccatatt tatcccgaaa atatttggat tgtaaaataa gagaacttta
     4381 tctaaattta accaaaaatc ttaaatgcaa cgaaatctca acctaaactt tacaaaattt
     4441 atttttgcgc tagctatact agtcagttta gtcccagcct gcaag
//
LOCUS       DAOQII010000214         4479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11629_length_4479_cov_4.810579, whole genome shotgun sequence.
ACCESSION   DAOQII010000214 DAOQII010000000
VERSION     DAOQII010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11629_length_4479_cov_4.810579"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLF48_09545"
     CDS             complement(<1..245)
                     /locus_tag="PLF48_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49611.1"
                     /translation="MFLHASTFVLIQKWSKKSRTIKGIFACTSGFSSKKSACENYLVA
                     CYLCLILVGFSAFELIAFHFILHDSFKSPRFFIASCC"
     gene            complement(521..841)
                     /locus_tag="PLF48_09550"
     CDS             complement(521..841)
                     /locus_tag="PLF48_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49612.1"
                     /translation="MKKLRGRMLLSSAKVMGKHHLFYGIDIDLVCVHMTAINLFLNGI
                     FHAEIMCADALRPGDFQVSYRTSLHPFGIFRITEKEQSPLWQMHQMEFKKTETQTGKQ
                     LSIF"
     gene            984..1526
                     /locus_tag="PLF48_09555"
     CDS             984..1526
                     /locus_tag="PLF48_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNF49613.1"
                     /translation="MAFLKNTSKNTINAVIFAISVVVPALVIAMLKFSPPEFTHSLNL
                     SFFPKFNAIVNTLTFFCLLAGFYFIKNKNIKAHKTMMLTALVLSCIFLLSYVIYHTLK
                     AEDSRYLGKTFLRYIYYFILITHIFLSLIVLPLVLKTFAFAFQDKIIHHRRWAKYTFP
                     LWLYVALSGVLVYVFMAPYY"
     gene            complement(1815..2789)
                     /locus_tag="PLF48_09560"
     CDS             complement(1815..2789)
                     /locus_tag="PLF48_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49614.1"
                     /translation="MQKLFLLALSSVLLTSCVSQKEYSKALDDYLNEAVKNKYLQLKL
                     DSVRQENIASQQQLQDKITQLRQDSATLTVEMAKQQAQITDMEDKFQSAQISFMKQLQ
                     QASSSNQKSNAELLQMQLDLEKQKLALDAKQATLNKLTEDVALRETRIQELEKLLNEK
                     NEKTQQLQLAVQQALSGFNAGELSVYQKDGKVYVSMSDNLLFKSASVKVEAKGEEALG
                     KLAEIIQKNPDLNIMIEGHTDNVPYKSNSSCMKDNWDLSLMRSSSVVHILTEKFKVNP
                     VQLIASGRGEFHPVSSNTTTEGKAQNRRTEIILTPNLDKLNQLLNSGK"
     gene            complement(2961..3395)
                     /locus_tag="PLF48_09565"
     CDS             complement(2961..3395)
                     /locus_tag="PLF48_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49615.1"
                     /translation="MKKVILISMLFAMTASYAQTNGISSFNSDLKGVWSLTISHVYNG
                     KVLSTTSNVYYFFSDFTFLVAPLAQSYSDILQVRPKLSSKWKIINNNLILFNPHGEQI
                     MAVFSKNNTEKIISGSKVIKGTHELNENDILKETSNWLVSLL"
     gene            complement(3503..>4479)
                     /locus_tag="PLF48_09570"
     CDS             complement(3503..>4479)
                     /locus_tag="PLF48_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49616.1"
                     /translation="LSDFGANYLFLKLNTNVWKINYQNIFAELTKFYGHGGDGLLTKK
                     YMAMHHLSINIGKHVNLGIFESVMFRRENNFELQYLNPIIFYRSIEQALGSPDNAFIG
                     FDMKANFAKHFQIYTQFLLDEFNFSKEFSRPGKSKLYSLFHPNKWWGNKFAAQLGLKY
                     IDAFGVDHLDLQLEGNIARPYTYSHYDTTTAWTHYNQPLAHPLGANFTELIFNVSYQP
                     MKQLYLNARFIYDRYGEDNDTTNWGGNPMKVYGTFEREYGNTTGQGIQARQFFVNFLA
                     TYEPWHNVFVDLEYTYRKKKSQDAVRNMQTHFLNLNLRWTLPYRRNEF"
BASE COUNT         1392 a          792 c          796 g         1499 t
ORIGIN      
        1 aaacagcatg aggcgataaa aaatcgtggc gacttaaaac tatcgtgcaa aataaaatga
       61 aaagcaataa gttcaaatgc gctaaatcca accaaaatta aacacaaata gcaagcgaca
      121 agatagtttt cacaagcaga ttttttagag ctgaagccac tggtacaggc aaaaatgcct
      181 tttattgttc ttgatttttt gctccacttt tgtatcaaga caaaagtgga agcatgcagg
      241 aacacggcaa caggaataac aaagctctat ttacttttaa taaatgccgg tgattacaga
      301 ccatctctat cgttttaaaa agcttttggt tgttttgata ccaatcaaac ccaaataaat
      361 gttccggctt tttatgcttc cagtcaaact tggtagttcg gggaatacgg aggagcatgt
      421 ctttaggtaa acaattcaaa taaaaggcta caataatagc aggatgatag gaggttttca
      481 tagtataaaa atagaaaaaa ccgcatagct gcggttgaag ttaaaagatg ctgagttgtt
      541 tgcccgtttg tgtttctgtt ttcttaaact ccatttgatg catctgccac aaaggagact
      601 gctctttttc ggtaatgcgg aatatcccaa aagggtgtag ggaagtgcgg tagctgacct
      661 gaaagtcgcc cggacgtagt gcatctgcac acattatttc tgcatgaaaa atgccattga
      721 gaaagagatt gattgccgtc atgtgcacac aaaccaaatc gatatcaata ccgtaaaaaa
      781 gatggtgttt gcccatcacc tttgcacttg agagcagcat tctaccccta agcttcttca
      841 taaccaagac ctaactttat taattcctgt tgctttaccc gaactcaagt taataaagtt
      901 aattttttca gcaagtattt aattaaagaa gtacggaaat aagtgtaagg aagttcgtaa
      961 tttgcgcctt ttaaatttcg gatatggcgt ttctaaaaaa tacatcaaaa aatacaatca
     1021 acgcagtaat ctttgccata tcggtagtag tgccggcatt ggtgattgcc atgctcaaat
     1081 tctctccacc tgaattcact cattctttaa acttatcatt ttttccaaag tttaatgcaa
     1141 ttgtcaatac actcactttt ttttgcctct tagccggatt ttattttatt aaaaataaga
     1201 acattaaggc gcacaaaacc atgatgctga cggcattggt attatcttgt atatttttac
     1261 tttcttatgt aatttatcac acgctcaagg ctgaagattc acgttattta ggaaaaactt
     1321 ttttgcgtta tatctattat tttattttaa ttacgcatat ctttttgtcg ttaatcgtgt
     1381 tgcctttggt tttgaaaact tttgcctttg cctttcagga caaaattatt catcaccgac
     1441 gctgggcaaa atacacattt ccgttgtggc tctatgtcgc actttccgga gtgttggttt
     1501 atgtatttat ggcaccttat tattaacctg agctcaggta aaaaatgaga agctaaaaaa
     1561 aatagttgat ttctcatttt cctccaactt ttaaaggagg aggaattaaa aggtggaggt
     1621 tctcaataaa aaatcaacta ttttgtaaaa atcaatctga gttcgtgtaa gaaaaagaaa
     1681 gtaaaaagaa tagttgattt ctcagtttcc tccaacttaa caaagaagat gaattaaatg
     1741 aaaaaaaatc aactatttac ttatccctat ctcaggttat taatttgggc tttgatttca
     1801 gtaagttaaa caatttattt tccggagttc aacagttgat tgagcttgtc taaattaggc
     1861 gtcaagataa tttctgttct tctgttttga gccttgccct cagttgttgt attgctggaa
     1921 acgggatgaa attcacctct gccggaagct attaattgta ccggattaac cttgaatttc
     1981 tctgtgagaa tatgcaccac cgagctggaa cgcataaggc ttaaatccca gttgtctttc
     2041 atacatgagc tgttcgattt atagggtaca ttatcggtat gtccttctat catgatattc
     2101 agatccggat ttttttgtat gatttcagca agtttaccta atgcttcttc gccttttgct
     2161 tctactttta cagaagccga tttaaataat aaattatcgc tcatcgacac atatactttt
     2221 ccgtcttttt ggtaaacaga caattcgccg gcattaaatc cggaaagtgc ctgttgtacg
     2281 gcaagctgta attgttgtgt tttctcgttt ttttcgttta ataatttttc gagttcttgt
     2341 atgcgggttt ccctcaatgc tacatcctct gtcagtttat ttagcgtggc ttgtttagca
     2401 tccagagcaa gcttttgttt ttctaaatcc aattgcattt gcaacaattc agcgtttgat
     2461 ttttgattgc ttgaagaggc ttgttgcaac tgtttcataa agctgatttg tgccgactgg
     2521 aatttatctt ccatgtcagt tatttgagct tgctgcttcg ccatttcaac agtaagtgtt
     2581 gctgaatctt gtcgcaattg tgtaatttta tcttgtagct gttgctgcga agctatattt
     2641 tcttgacgaa cggaatcgag ttttaactga agatatttat tctttaccgc ctcattcaga
     2701 taatcatcga gtgcctttga atattctttt tgagaaacac atgaagtaag taatacacta
     2761 gaaagtgcaa gcaaaaaaag tttttgcata atttattttt ctgaaaaata gttatgatgt
     2821 tgctgtatta ataactgaaa ctataaacga atgtggattt aaagcaagag aatatttatc
     2881 aaataaaata aagtagtagt gtttgttaaa ataaaaaatg gggattgcga agcaatcccc
     2941 accgacgact aactaagtaa ctaaagcaag gagactaacc aattgcttgt ttctttcaat
     3001 atatcatttt cgtttagttc atgagtaccc ttgattacct tagaaccgga aataatcttt
     3061 tctgtattat tctttgaaaa aacagccata atctgctctc catgaggatt aaacagaata
     3121 agattgttgt tgattatttt ccattttgat gaaagtttag gacgtacttg caaaatgtct
     3181 gaataacttt gagccaacgg agcaacaagg aatgtaaaat cagaaaagaa gtaataaaca
     3241 ttacttgtgg tagaaagtac ttttccattg taaacatgtg aaatggttaa actccatact
     3301 ccttttaaat cggaattaaa agagctaatg ccattggtct gcgcatagct cgctgtcata
     3361 gcgaacaaca tgctgattaa aattactttt ttcatagtta tttgttttgt tggtttaaaa
     3421 ttatgctaaa cccaactcaa caacaacttt acttcgttaa tggtagaaaa aaatgagtaa
     3481 acggtaaaaa gcagtatcct aattaaaatt cattccttct gtatggaagt gtccagcgca
     3541 aattcagatt tagaaaatgt gtttgcatat ttctgacggc atcctggctt ttctttttgc
     3601 gataagtata ttccagatcg acaaatacat tatgccaggg ttcgtaggtg gcaaggaagt
     3661 ttacaaaaaa ttgtcttgct tgtatacctt gtcctgtagt atttccgtac tctcgctcaa
     3721 aagttccgta aactttcatc gggttgccac cccagttggt cgtatcgtta tcttcaccat
     3781 atctatcata aataaagcgg gcattcagat acaattgttt catgggctga tagctgacat
     3841 taaaaatcaa ttcagtaaaa ttagccccaa gtggatgagc taaaggttga ttgtagtgcg
     3901 tccacgccgt tgtagtatca taatgcgaat aggtataagg tcgggcaata tttccttcca
     3961 gttgtaagtc cagatggtca accccaaatg catcaatgta ttttaatcct aactgtgcgg
     4021 caaatttatt tccccaccat ttattcggat gaaacaagct gtacagtttg ctttttcccg
     4081 gacgactaaa ctcttttgaa aaattaaatt cgtctaataa gaactgggta taaatttgaa
     4141 aatgtttggc aaaatttgct ttcatgtcaa aaccgataaa tgcgttatcg ggagaaccta
     4201 aagcctgctc gatggaacgg taaaaaataa tcggattcag gtattgtaat tcaaaattat
     4261 tctcgcgtct gaacatcacg ctttcaaata tcccgaggtt tacatgtttg ccaatattta
     4321 tactcaggtg atgcatggcc atgtattttt tagtcagcaa gccatcgcca ccatgaccat
     4381 aaaatttagt caattctgca aaaatattct ggtagtttat tttccaaaca ttcgtattca
     4441 gctttaagaa taaataattt gctccaaaat cagataaga
//
LOCUS       DAOQII010000215         4458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11700_length_4458_cov_4.098569, whole genome shotgun sequence.
ACCESSION   DAOQII010000215 DAOQII010000000
VERSION     DAOQII010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11700_length_4458_cov_4.098569"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLF48_09575"
     CDS             <1..230
                     /locus_tag="PLF48_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNF49617.1"
                     /translation="CQTCHGKVEEYEVMKQFAPLSMGWCINCHRQTEVKFAENKYYSV
                     FQKYQDELKKGTKAKVTVEDIGGTECQKCHY"
     gene            261..3356
                     /locus_tag="PLF48_09580"
     CDS             261..3356
                     /locus_tag="PLF48_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNF49618.1"
                     /translation="MSNKKYWTSLDELHETPRYKEQAAKEFNFEVDTTETDDNNGTSR
                     RDFLKAMGFSVSVAALASACKIPTHKAIPYTIDTRKAVPEVVPGVADYFASTFYDGND
                     CINILVKTREGRPIKIEGNNDSPLTKGGTSARAQASVLGLYDTTRLATPTANNKKTDW
                     KTVDAEITQKLDTIVKGGGKIALVTGTVASPITKKAIEKFVAKYPSTTVVAYDAISNY
                     ATRKANQISLGAAIIPSINYDKADIIVGFNADFLGTGISSIENTKKYIANRVPTQENP
                     KMSRHHQFQSTMTITGASADYRYPINPSEEKQALINLLAAIGGTISGSKSLAGKDANE
                     GIAKVAKELTAAKGKSVVVSGTNDVEIQLLVNAINSTLGNYGAVIDTANAYNLKSGND
                     EALATLANDLKSGAYKGIILLGTNPVYDTQYADIFKDAIKAAEVSVSTADRADESAQF
                     CKYICPNAHYLESWDIINPKPGFYGFVQPTINPLFDTRQTQASLLTWAGDTTPIGQFV
                     QNEISTIVGGNYQDAVQLGYFGTVATGSGASANAASAIAAVKTDVAAPKGLEVLFYEK
                     VGLGDGRYANNPFLQEMPDPIAKVTWDNYVCISYSYAKAKGIDPQPSEKRVPIAKVTI
                     NGKSIELPVVVQFGQPANTLAIALGYGREKAGKAGDMVGKNVFPFLKNINGATSYTLD
                     GATIEMTGKVYELGLNQTYHTLMEDNNLPNRKKHYRGEIVKETTLAAYQKNEHAGNED
                     REKIKEEFGHSLYDGYQPFDYKQGHHWGMTVDLNACIGCGACVVSCNIENNIPVVGKR
                     QVFRSQEMHWMRIDRYYNGNPDNPDVSFQPMMCQHCNNAPCENVCPVNATNHSSEGLN
                     QMAYNRCIGTRYCANNCPYKVRRFNWLDYQGVDSFGKFNDGKRSFTDTEAQSYMFEDL
                     TRMVLNPDVVVRSRGVIEKCSFCVQRIQEGKLNAKKAGRPLQDGEIKTACQTACPTNA
                     ITFGDQNNKEGAVAKTFNTNGRNYFVLEEQHFIPAVGYQVKVRNKDEEPAEYFIFKPE
                     A"
     gene            3387..>4458
                     /gene="nrfD"
                     /locus_tag="PLF48_09585"
     CDS             3387..>4458
                     /gene="nrfD"
                     /locus_tag="PLF48_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNF49619.1"
                     /translation="MASHFESPLREPLIFGNKTYRQITQDIVRPTTGKIQPKWLFWTM
                     ISASVAGLFVFCITWEVCFGIGTWGANKTVGWAWDITNFVWWIGIGHAGTLISAILLL
                     FRQKWRTGVNRAAEAMTIFAVICAGQFPVFHMGRVWDAFFIFPYPNTRGPLWLNFNSP
                     LLWDVFAISTYFTVSLLFWYTGLVPDFGTIRDQSKTPGRWKFYNFLSFGWVGSAKHWE
                     RWESLSLILAGLSTPLVLSVHTIVSFDFATSVVPGWHTTIMPPYFVAGAIFSGFAMVL
                     TLMLVARHVMDLKEYITLAHIESMNKIMLLTGTMVGIAYLTELFTAWYSGNLFESYAF
                     ANRAFGPYWWAYWSMMFCNLVSP"
BASE COUNT         1497 a          819 c          911 g         1231 t
ORIGIN      
        1 aatgccaaac ttgccacggt aaagtagaag agtacgaagt aatgaaacaa tttgctcctt
       61 tgtcaatggg atggtgtatc aactgccacc gacaaacgga agtgaaattt gcagaaaata
      121 aatattattc tgtattccaa aaatatcaag acgaattgaa aaaaggtact aaagcgaaag
      181 taaccgtaga agatatcggt ggtactgaat gtcaaaaatg tcattattaa tattcgagaa
      241 ctcaattaac ctttatataa atgagtaaca aaaaatattg gacgagctta gatgagctgc
      301 acgaaactcc tcgatacaaa gaacaagcag cgaaagaatt taattttgaa gtagatacta
      361 cagaaacgga cgacaacaat ggcaccagcc gtcgcgattt cttaaaagca atgggattta
      421 gtgtttctgt tgctgcattg gcatctgctt gtaaaattcc aacccataag gcaatccctt
      481 acactattga cactagaaaa gcagtaccgg aagtagtgcc cggagtagca gattattttg
      541 cttctacatt ctatgacggt aatgactgca ttaatatctt agtaaaaaca cgtgaaggcc
      601 gccctattaa aattgaagga aataatgatt ctccgttaac aaaaggagga acaagtgcaa
      661 gagcgcaagc ttcagtatta gggttatacg atactactcg cttagcaacc cctactgcca
      721 acaataaaaa aacagattgg aaaacagtag atgctgaaat tactcaaaaa ttagacacaa
      781 tagtaaaagg cggaggtaaa attgctttag taaccggtac agttgcatcg cctattacca
      841 aaaaagcaat agagaaattt gtagcaaaat atccttctac aacagtagtt gcgtatgatg
      901 ctatctctaa ttatgctacc agaaaagcca atcaaatcag tttaggagca gccattatcc
      961 catctatcaa ttatgataaa gcagatataa ttgttggttt taatgcagat ttcttgggta
     1021 ccggaatttc ttctattgag aatacaaaaa aatatatcgc aaacagagtt cctacccaag
     1081 agaatcctaa aatgtctcgc caccaccaat tccaatctac gatgacgatt acaggtgcaa
     1141 gcgcagatta cagatatcca attaacccat cagaggaaaa acaagcatta atcaatttat
     1201 tagcagccat aggcggtacg ataagcggct ccaaaagcct tgccggaaaa gacgctaatg
     1261 agggtattgc aaaagtagca aaagaactaa ctgctgctaa agggaaatct gttgttgttt
     1321 cagggacaaa tgacgtagaa atacaattat tagtgaatgc gataaacagt acattaggaa
     1381 actatggtgc tgtaatagat actgcgaatg cctataattt aaaatcgggt aatgatgaag
     1441 cattagctac attagcaaat gacttaaaat ccggcgcata caaaggtatt atcttgttag
     1501 gtacaaatcc tgtgtatgat acccaatatg cggatatttt taaagatgcg attaaagccg
     1561 cagaagtttc tgtatctaca gcagatagag cagatgaatc agcgcagttt tgtaaatata
     1621 tctgtccaaa cgcacactac ttagagtctt gggatatcat taatccaaaa ccgggtttct
     1681 atggctttgt acaaccaaca attaatccat tattcgacac gcgtcaaact caagcttctt
     1741 tattaacttg ggctggagat accacaccaa tcggacaatt tgtacaaaat gaaatttcta
     1801 ctatcgttgg cggtaattat caagatgccg ttcaattagg ttatttcgga actgtggcaa
     1861 ccggtagcgg tgcatccgca aatgccgctt cagcaattgc cgcagtaaaa acagacgttg
     1921 ctgctccaaa aggattagaa gttctctttt atgaaaaagt aggtttaggc gatggtcgtt
     1981 atgcaaataa tcctttttta caagaaatgc ccgatccaat cgcaaaagta acttgggata
     2041 actatgtatg tatttcatat tcttatgcaa aggctaaagg tatcgacccg caaccttctg
     2101 aaaaaagagt accaattgca aaagttacta taaatggaaa atcaattgaa ttaccggtag
     2161 tcgttcaatt tggtcagccg gcaaatacat tggcaatagc tttagggtat ggtcgtgaga
     2221 aagcaggaaa agccggcgac atggtgggta aaaatgtatt cccattcctg aaaaatataa
     2281 atggtgcaac ttcttatacg cttgatggtg caaccatcga aatgacaggc aaagtttatg
     2341 aattaggatt aaaccaaacc tatcatacgt taatggaaga caacaacctt ccaaaccgta
     2401 aaaagcacta tagaggggaa atcgttaaag aaacgacatt agcagcatat caaaaaaatg
     2461 aacatgcggg taatgaagat agagaaaaaa taaaagaaga atttggacat tctctatatg
     2521 atggctatca accattcgac tataaacaag gtcaccattg ggggatgact gtcgatttga
     2581 atgcttgtat cggttgtgga gcttgtgtcg tatcttgtaa tatagaaaac aatattccgg
     2641 tagtaggtaa acgtcaagta ttccgctctc aagaaatgca ttggatgcgt atcgaccgtt
     2701 actataacgg caacccggat aatccggatg tatccttcca accgatgatg tgtcagcatt
     2761 gcaacaacgc accatgtgaa aacgtgtgtc cggtaaatgc aacgaaccat agttcagaag
     2821 gattgaacca aatggcatac aacagatgta tcggtacaag atattgtgct aataactgcc
     2881 catataaagt acgccgcttc aactggttag attatcaagg agtagatagc ttcggtaaat
     2941 ttaatgacgg caaacgctca ttcaccgata cagaagcaca gtcttacatg tttgaagatt
     3001 taacaagaat ggtgttgaac ccggatgttg tagtacgctc tcgcggggta attgaaaaat
     3061 gttctttctg cgtacaacgt atccaagaag gaaaattaaa tgcaaaaaaa gcaggacgcc
     3121 ctttacaaga tggcgaaatt aaaactgctt gccaaacagc atgccctaca aatgccatca
     3181 cttttggaga ccaaaataat aaagaaggag ctgttgcgaa aacattcaat acgaacggta
     3241 gaaactattt tgtgttagaa gaacaacatt ttattccggc tgtaggatac caagtgaaag
     3301 ttagaaataa agatgaagaa ccagcagaat atttcatttt taaaccagaa gcataatcat
     3361 taataaaagt tgtaaagtaa gatataatgg caagtcattt tgaatcacca ctacgtgaac
     3421 cgttaatatt cggtaataaa acgtacagac aaattaccca agacatcgtt agaccaacga
     3481 cgggtaagat tcaacctaaa tggttgtttt ggacgatgat atcggcatct gttgcagggt
     3541 tatttgtttt ctgtattaca tgggaagttt gcttcggtat tggtacttgg ggcgcaaata
     3601 aaacagtagg ttgggcttgg gatattacca acttcgtttg gtggatcggt atcggccacg
     3661 ccggaacatt aatttcagct atcctattat tattccgtca aaaatggcgt accggtgtga
     3721 accgtgctgc tgaagcgatg acaatatttg ccgtaatatg cgccggtcag tttcctgtct
     3781 tccacatggg acgcgtatgg gatgctttct ttattttccc ttaccctaat acaagaggcc
     3841 cgctttggtt gaattttaac tcaccacttt tatgggacgt gtttgccata tctacatatt
     3901 tcactgtttc actattgttt tggtacacag gtttagtgcc tgattttggt acgattcgcg
     3961 atcaatccaa aacaccggga agatggaaat tctataattt cttaagcttt ggttgggtag
     4021 gaagtgcaaa acattgggaa cgttgggaat cattgtcttt aattttagca ggtttatcca
     4081 ctccattagt attatccgta cataccatcg tatcatttga ctttgctaca tcagtagtgc
     4141 cgggctggca taccactatc atgcctccat actttgtggc aggagcgatt ttctccggat
     4201 ttgcaatggt attgacattg atgttggtag caagacatgt gatggatttg aaagaatata
     4261 tcacattagc gcatatcgaa tcgatgaaca aaatcatgtt gctaaccggt accatggtag
     4321 gtatagctta tttaacggag ttatttacag cttggtactc aggtaatctt ttcgagagtt
     4381 atgcattcgc caatagagct ttcggaccct attggtgggc atattggtca atgatgttct
     4441 gtaacttagt atctcctc
//
LOCUS       DAOQII010000216         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11790_length_4438_cov_3.224504, whole genome shotgun sequence.
ACCESSION   DAOQII010000216 DAOQII010000000
VERSION     DAOQII010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11790_length_4438_cov_3.224504"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="PLF48_09590"
     CDS             <1..1301
                     /locus_tag="PLF48_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49620.1"
                     /translation="LKSRFKQRLYNTLYFLDLNQTNLADLAKKDYAFCLNKLYLSNLL
                     LRYADNRNASITLVMEAYPIAQKYNFYDLLKEFSFKLLIFYGMNGKTKQYNEEIIRYE
                     NFKKEYELEQSAQLIYSKIYLMSNMTKYDPEILGQQMLVEIDKLIPLYQASSSINTYF
                     SYIRSKLFYFEKFADNVNMIAVCDEFLKNYKNYKSSLLLQNYTDVISLHKLKSLFDVR
                     ELQKSNELAMQLESNARSLNWLTVKEFQFKANLNNFNRHVLNLAEEVIGSMHYKSASN
                     AIKERWLLYQAYAMLMLQYINQTPYKINIKKLDNQLETISQDKTGFNLSFRILEIVYA
                     ISLNDLDMAADKITLLEAYQKRHIKQEKYDRFILFLSLLIQAEKRGFNYKELARLKPY
                     LSLKENFRMQYINMNEIVFFDKLWEIILDMLKTNDNKLFA"
     gene            complement(1313..2860)
                     /gene="hutH"
                     /locus_tag="PLF48_09595"
     CDS             complement(1313..2860)
                     /gene="hutH"
                     /locus_tag="PLF48_09595"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNF49621.1"
                     /translation="MAEVFHYGTDQLTVRICMALTEGKITGVLSPAAKEKIVKSQHDV
                     LEIVENDETVYGINTGFGILANKKISKADAELLQYKILQSHSVGIGNPIPKKIAKTML
                     ITKLHALAQGFSGVQLTTLERILWMIDEDIIPLVPEKGSVGASGDLAPLAHLFLPLIG
                     MGEVYYKEKITNTQAVLQEKNMEPVLLGPKEGLALINGTQFILSYAVHDVYRLHYCLE
                     AADIAGAMSLEALQGSKRPFETKLHELRPYAGSLHVAKHLQSLLENSEIMEAHEDCER
                     VQDPYSLRCMPQVHGASRNAWLHLKELTEIELNSVTDNPVIFSADDTISGGNFHGQPL
                     ALPLDYACFAASELGNIADRRCYLMLEGKFGLPMLLMNDVGLNSGFMIPQYTTAALVT
                     ENKTLCFPASADSIPTSLGQEDHVSMGSISGRKLYQILDNIETILAIELMCAAQAIEF
                     RRPLKSSSILEAAHNFIRDKVSFANEDRIFANDIKVLKSCLEDFSFVDYISKKSSIEK
                     EKISFQP"
     gene            complement(2903..3382)
                     /locus_tag="PLF48_09600"
     CDS             complement(2903..3382)
                     /locus_tag="PLF48_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49622.1"
                     /translation="MRHKIWFGFLSSLLLFNACYKTPEYPDTPAIEFDSYTVAQPYTV
                     GDPGYIRINFTDGGGDFGRLNNSTVDSSCFITNSIFHTTLKYVIPVIPKNGTSDNISG
                     TIDIDLKSVLDSLGCEGILYQYDYPFDTVSYEIVVKDRSGHYSNTITTPPLIFKCRN"
     gene            3404..4387
                     /locus_tag="PLF48_09605"
     CDS             3404..4387
                     /locus_tag="PLF48_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNF49623.1"
                     /translation="MAEAILENIFDSVNFSFEKTALSVFRYQYTHNPVYKQFVDLLKV
                     NPDDVQTMEKIPFLPIEFYKSHKIITDNAEIQKVFESSGTTDSIRSKHFVADLNLYKQ
                     AAVSIFEQTYGDLSQWTILALLPSYLERDNSSLVYMVDYFIKKTQAVDSGFYLDHPEQ
                     LVQKLLSLKEQGKKVLLIGVTFALLDLAEQFPADLSGITIMETGGMKGRREDMQRSEI
                     HAILSNAFRVNVIHSEYGMTELLSQAYSKGNGIFEDSRYLKVLKREINEPTKVSASVG
                     RGGLNVIDLANIYSCSFIATEDVVNILPNNQFEVLGRLDYAEMRGCNMLVI"
BASE COUNT         1446 a          807 c          782 g         1403 t
ORIGIN      
        1 gactgaaatc gcgtttcaaa cagcgattat ataatacctt atattttctc gatttaaacc
       61 agaccaattt agccgatttg gctaagaagg attatgcatt ttgccttaat aaactctatc
      121 tcagcaatct ccttttgcgt tacgccgata accgcaatgc atcaattacg cttgtaatgg
      181 aagcctatcc gattgctcaa aaatataatt tttatgactt gttaaaagag ttttcattca
      241 agttactcat cttctacggg atgaatggta aaactaaaca atacaatgaa gaaataatcc
      301 gatatgaaaa ttttaaaaaa gaatatgagt tagaacaaag tgcccagctg atttactcga
      361 aaatctatct gatgagtaat atgaccaagt acgaccctga aattttagga cagcaaatgt
      421 tagtcgaaat agataaactg ataccacttt atcaggcatc atcttctata aatacttatt
      481 tcagctatat ccggagtaaa ctattttatt ttgaaaaatt tgctgataat gtcaatatga
      541 ttgctgtttg tgatgagttt cttaaaaatt acaagaacta caaaagttca cttttgctcc
      601 aaaattatac ggatgtaatt agcctgcata agctaaaatc actgtttgat gtgcgcgaat
      661 tacaaaaaag caatgaactt gccatgcaac tcgagtcgaa tgccagaagt ttaaactggc
      721 ttacggtaaa agaatttcaa tttaaagcga acttaaacaa ttttaaccgg catgtgctga
      781 accttgccga agaagtgatt ggcagtatgc actataaatc tgcatcaaat gccataaaag
      841 aacgctggtt actctatcag gcttatgcta tgcttatgct gcagtatatc aaccaaactc
      901 catataaaat aaatataaaa aaactggata accagcttga aaccatctcc caagataaaa
      961 ccggatttaa tctttctttc cgtattcttg aaatagttta tgccatctca ttaaatgatt
     1021 tggatatggc agccgacaaa attacactgt tagaggctta tcaaaaacga catatcaaac
     1081 aagaaaaata tgaccgcttt atcctgtttt taagtttatt gatacaagct gaaaaacgcg
     1141 gttttaacta taaagaactt gccagattaa agccgtattt gtcacttaaa gaaaatttca
     1201 gaatgcagta tatcaacatg aatgagattg tcttttttga taaattatgg gaaataattt
     1261 tggatatgct gaaaactaat gataataagc tgtttgccta aaggtgtttc tatcacggtt
     1321 gaaaactaat cttctctttt tctatacttg atttttttga aatataatca acaaagctaa
     1381 agtcttccaa acaagatttt aaaactttta tatcattggc aaaaatgcgg tcttcattag
     1441 caaatgaaac tttatcacga atgaaattat gcgcagcttc taatatagat gagcttttta
     1501 atggtcgtct gaattctatg gcttgtgctg cacacatcaa ttctatagct agtatggttt
     1561 caatattatc tagaatctga tacagtttgc gtccactgat actgcccatg ctgacgtgat
     1621 cttcttgccc taatgatgtt ggaatactgt cagcacttgc cggaaaacag agcgttttat
     1681 tttcagtaac caaagcagct gtagtatatt gcggaatcat aaagccgctg tttaggccta
     1741 catcattcat taacagcata ggtaatccga atttaccttc cagcatcaaa taacatcttc
     1801 ggtctgctat attacccaac tcagaagcag caaaacaagc ataatctaat ggcaatgcca
     1861 aaggttgtcc atgaaagttt ccgccactga tagtatcatc agctgaaaaa ataaccggat
     1921 tgtccgttac cgaatttaat tcgatttcgg taagctcttt caagtgcagc caggcatttc
     1981 tcgaagcacc gtgcacttga ggcatacagc gcaaactata ggggtcttgc acacgctcac
     2041 agtcttcatg tgcttccatt atttcagaat tttccaagag cgattgcagg tgttttgcta
     2101 cgtgcagact tccggcataa gggcgaagtt catggagctt agtttcaaat ggacgcttcg
     2161 agccttgtaa agcttcgagg ctcatcgctc cggctatatc agctgcttcg aggcaataat
     2221 gcaaccgata cacatcatga acggcgtagg ataaaataaa ttgtgtgcca tttatcaatg
     2281 caagtccttc tttaggaccc agtaataccg gttccatatt tttttcctgt aatacagctt
     2341 gcgtatttgt aatcttttct ttgtaataaa cttcgcccat gcctatcagt ggcaaaaaca
     2401 aatgggctaa aggagctaag tcaccactgg cacctacact gcctttttcg ggtactaaag
     2461 gaataatgtc ttcatcaatc atccataaaa tgcgctctaa tgtggtaagt tgcacgcccg
     2521 aaaaaccttg cgctaaggca tgcaatttag taatcagcat ggttttagct atttttttcg
     2581 gaatgggatt gccgatacct acactatgcg attgtaaaat tttatactgc agtaattcag
     2641 catctgcttt agatattttt ttattagcca aaatgccgaa accggtattg atgccatata
     2701 ccgtttcatc attttctaca atttcaagta catcgtgttg agattttaca attttttctt
     2761 ttgcagcagg acttaaaact cctgtgattt tgccttccgt taaggccatg caaatgcgaa
     2821 cagtaagctg atctgttcca taatgaaata cttctgccat gaagtaaaag tacaaaaaaa
     2881 gtaaatgttg cttaaatgtc ttttaattgc ggcatttaaa aattaacggt ggtgttgtta
     2941 tggtattgct gtagtgacct gagcgatctt tcaccactat ttcataactt acggtatcaa
     3001 aaggataatc gtattggtat aaaatacctt cgcaccccag tgaatctaaa acggatttta
     3061 aatcaatatc gatagtaccc gaaatattgt ctgatgttcc gtttttagga attaccggaa
     3121 tgacatattt aagcgtagta tggaaaatac tattcgttat aaaacaagat gaatctaccg
     3181 tagaattatt gagcctgcca aaatctccgc ctccgtctgt aaagttgatt ctgatatatc
     3241 ccggatcgcc aacagtataa ggttgtgcta ccgtataaga atcaaattca atagccggag
     3301 tgtccggata ttcaggtgtt ttataacaag cattaaacaa aagcaaactg cttaaaaaac
     3361 cgaaccaaat tttgtgtcgc atagttgtat ctttatgcct tttatggcag aagcaatttt
     3421 agaaaatatt tttgacagtg tcaatttttc ttttgaaaaa actgcacttt ctgtatttcg
     3481 atatcaatat acgcataatc ctgtatataa gcaatttgtc gatttgttaa aagtaaatcc
     3541 ggatgatgta caaacgatgg aaaaaattcc gtttttacct attgagtttt acaaatcaca
     3601 caaaatcatt acagataacg cagaaattca aaaagtattt gaaagttcgg gtacgactga
     3661 cagcatcaga agcaaacatt ttgtagcaga tttaaatttg tataagcaag cagcggtttc
     3721 catatttgaa caaacttatg gtgatttaag tcaatggact attttagcct tattgccttc
     3781 ctatcttgaa cgagataatt cttcgctggt ttatatggtt gattatttta tcaaaaaaac
     3841 acaagctgtt gattccggat tttatcttga tcatcccgag caattagtac aaaaactatt
     3901 atccttaaaa gaacaaggca aaaaagtgct actgattggt gtcacctttg cacttttgga
     3961 tttagccgaa caatttcctg ccgatttatc aggaattacc atcatggaaa ccggagggat
     4021 gaagggcagg agagaagata tgcagcgttc tgaaattcat gcaatactgt ctaatgcatt
     4081 tcgggtaaat gtcatacatt cagaatatgg catgaccgaa ttattatcgc aagcctattc
     4141 gaaaggaaat ggaatttttg aagattctcg ttacctgaaa gttttaaaac gagaaatcaa
     4201 tgaacctact aaagtttcag catcagttgg cagaggaggc ttaaatgtaa ttgatttggc
     4261 aaatatctat tcctgtagtt ttatcgccac agaagatgtt gtgaatattc ttcccaataa
     4321 ccagtttgaa gtattgggca gactagacta tgctgaaatg cgaggctgta atatgcttgt
     4381 aatctgagtt cgggataagc atttcaatta aaaaatagtt gattttttat gtagaacc
//
LOCUS       DAOQII010000217         4418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11861_length_4418_cov_4.537245, whole genome shotgun sequence.
ACCESSION   DAOQII010000217 DAOQII010000000
VERSION     DAOQII010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11861_length_4418_cov_4.537245"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLF48_09610"
     CDS             <1..949
                     /locus_tag="PLF48_09610"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNF49624.1"
                     /translation="EKDIKPSDIMTFKAFENAIRMVIVLGGSTNAVMHLIAMAKTIGV
                     QLTLVDFTRIANETPVLADFRPSGKYVMEDLHKIGGVPAVMKLLLKNGLLHGDCLTVT
                     GKTLAENVADLPDLKAGQDLIFPLDQPIKKTGHIRILYGNLATEGAVAKITGKEGERF
                     EGNARCFDNEQAAIDAMQRKEIKAGDVVIIRYIGPRGAPGMPEMLKPTSVLIGEGLGK
                     EVALITDGRFSGGSHGFVVGHVTPEAQNGGNIALIKDGDKIKIDAVNNTIDVLISVEE
                     MSMRRANWKAPELKAKNGVLYKYAQSVSSASEGCVTDRT"
     gene            complement(972..2906)
                     /gene="dnaG"
                     /locus_tag="PLF48_09615"
     CDS             complement(972..2906)
                     /gene="dnaG"
                     /locus_tag="PLF48_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNF49625.1"
                     /translation="MITRDTIQRIYDTVQIEDVVRDFVPLKKVGSIFKACCPFHQEKT
                     PSFAVNPNKNIYKCFGCGKGGDSVNFVMEHEKMTFPEALRYLAQKYNIQVEETFSENE
                     NNKEVQDDRESLLIVLNYATQFFKLQLFETEDGKSIGLPYFKERGFTENTINEFQLGY
                     AIDSFDNLYKDAVSKGYNKDLLLKAGLIKEKNEKYYDFFRDRVMFPIHNVSGKTIAFG
                     GRILKKAENQPKYINTPETDVYHKSQIVYGIFQAKAEIRKHDVCFLVEGYTDVISLHQ
                     AGIKNVVASSGTALTKEQVLLIKRFSENMVILYDGDAAGVKAALRGLDIVLEQGMNVK
                     IVLLPDNEDPDSFVKHRGADETLRFIETHQQDFIFFKTNIGIKETENDPAKKAEVIRD
                     IVESIALIGDNIKRQLYIKQCADIVDVPEAILVNEINKIRTQQLKKNRIINEDEKAQL
                     NEHITEEIEHKQMHTKIPFLYYQERDIIRILLEYGDKEMTEENNVAEYVIFEMFDVEL
                     KNNVFGKIIRLYIDAYQNEIPFEDIKKPQNIEDPEVKETLIELMSSPHELSPNWFNKH
                     EIIVRDANRTYKTDVVSVMNRYRYYMLMEVMKILDERIKTAETSGNFEEMIASLLKKM
                     EYMETRKTLAKEIQTVIHPF"
     gene            complement(3289..3489)
                     /locus_tag="PLF48_09620"
     CDS             complement(3289..3489)
                     /locus_tag="PLF48_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49626.1"
                     /translation="MDKSKKIVWSQKAEDDLMQILEYWFNRNKSIVFTEKLTVEIFEA
                     IQILKTHPNFGRMINQAEFRRV"
     gene            complement(3468..3686)
                     /locus_tag="PLF48_09625"
     CDS             complement(3468..3686)
                     /locus_tag="PLF48_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49627.1"
                     /translation="MTIKDDIILKIQNSDDVQLLAEVFRILKADEYVFSDEEIASFYV
                     ADTAIDEGRYIDNELLNSKVKAWINQKR"
     gene            complement(3919..>4418)
                     /locus_tag="PLF48_09630"
     CDS             complement(3919..>4418)
                     /locus_tag="PLF48_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNF49628.1"
                     /translation="LTIRPTLDVNGIWGGYIGEGAKTVLPAKASAKISMRLVPNQSSK
                     KIAELFTKYFEKIAPETVTVKVTEHHGGEPYVTPVDSVEFVAAYKALEHTFGKKPLAM
                     RNGASIPIVALFEDILGLKSVLMGFGLDSDNIHSPNEHYGLFNYFKGIETIPYFYKYY
                     AELRG"
BASE COUNT         1327 a          825 c          769 g         1497 t
ORIGIN      
        1 tgaaaaagac attaagccca gcgatattat gacgtttaaa gcatttgaaa atgccattcg
       61 catggtcatt gttttaggtg gaagtacaaa tgccgtgatg catcttattg ccatggcaaa
      121 aaccattggc gttcaactca cacttgtcga tttcactcgt attgccaatg aaactccggt
      181 gcttgccgac ttcagaccga gtggaaaata cgtgatggaa gacttgcata aaatcggtgg
      241 tgttccggca gtgatgaaac tacttttgaa aaacggactt ttgcatggcg actgcttaac
      301 tgttacagga aaaacactgg cagaaaatgt ggctgactta ccggatttaa aagcaggtca
      361 ggatttaatt tttccattag accaaccaat caaaaaaaca ggacatatcc gtattttata
      421 tggcaactta gctactgaag gtgccgtggc aaaaataacg ggaaaagaag gcgaaagatt
      481 tgaaggcaat gcccgttgtt ttgataacga gcaagctgct attgatgcta tgcaacgcaa
      541 agaaattaag gcaggtgatg tcgttattat tcgctatata ggcccgagag gtgcacccgg
      601 tatgcctgag atgctaaaac cgacatcagt acttattggt gaaggcttgg gcaaagaggt
      661 ggcattaatc acagacggca gatttagcgg aggttctcat ggctttgtag tcgggcacgt
      721 tactcctgaa gcacaaaacg gtggaaatat cgcactgata aaagatggcg acaaaataaa
      781 aattgatgct gtcaataata cgatagacgt attgataagt gttgaagaaa tgagcatgcg
      841 aagagccaac tggaaagcac ctgaattaaa agctaaaaac ggtgtgctgt acaaatatgc
      901 acagtcggtt tcatcggcca gcgaaggctg tgtaactgat agaacataat tgatataagt
      961 ttatatttcc ttcaaaaggg atgaatgacc gtttgaattt ctttggctaa ggttttgcgg
     1021 gtctccatat attccatttt tttcagtaaa gaagcaatca tttcttcaaa attgccgcta
     1081 gtttctgctg ttttaatacg ttcatccaaa attttcatca cttccatcaa catataataa
     1141 cggtagcggt tcatcactga aactacatcc gttttataag tgcggttggc atcacgcaca
     1201 ataatttcat gtttattaaa ccagttagga ctcagttcat gcggtgaact catgagttca
     1261 atcaatgttt cttttacttc cggatcttca atattttgag gttttttgat gtcttcaaaa
     1321 ggtatttcat tttgataggc atcaatatat aaacgaataa tttttccgaa cacattattt
     1381 tttaattcca catcaaacat ttcaaaaatc acatactctg ccacattatt ttcttccgtc
     1441 atttctttat ctccatactc gagtaaaatg cgaataatat ctctttcctg ataatacaaa
     1501 aatggaattt tagtatgcat ttgcttgtgc tcaatttctt cggtaatatg ttcgtttaat
     1561 tgagcttttt catcctcatt gataattcgg tttttcttga gttgttgtgt gcgtatttta
     1621 ttgatttcat ttactaaaat cgcttccgga acatctacaa tatccgcaca ttgtttgata
     1681 tacaattggc gtttgatatt atcgccaatc aatgcaatac tttccacaat atcccgaatg
     1741 acttccgctt ttttagccgg atcattttct gtttccttta taccgatgtt tgtcttgaag
     1801 aaaataaaat cctgctgatg tgtttcaata aaccgtaagg tttcatcagc gcctcggtgt
     1861 ttgacaaaag agtccgggtc ttcattatcc ggcaacaata cgattttcac attcatgcct
     1921 tgctccagca cgatatccaa tccgcgtaat gcggctttca cgccggcggc atcaccgtcg
     1981 tagaggataa ccatattttc tgaaaatcgt tttatcagca aaacttgttc tttggtcaat
     2041 gccgtaccac ttgatgccac tacattttta atgcctgcct gatgcaaaga aataacatcg
     2101 gtataacctt ctaccaaaaa acaaacatcg tgtttgcgta tctccgcttt agcctgaaaa
     2161 atgccataaa caatctgact tttatgataa acatccgttt ccggcgtgtt tatatatttt
     2221 ggctggtttt ctgctttttt gagaatacgt ccgccgaaag caatcgtttt gccgcttaca
     2281 ttgtggatgg gaaacattac acggtcgcgg aaaaaatcgt aatatttttc gtttttttct
     2341 tttatcaatc cggctttcag cagcagatct ttattatagc cttttgaaac agcatcctta
     2401 tataaattat caaaactatc aattgcataa cccagttgaa attcgtttat cgtattttca
     2461 gtaaatcctc tttctttaaa ataaggcaag ccgatggatt tgccgtcttc tgtttcaaag
     2521 agttgcagtt tgaaaaattg cgtggcataa tttaatacga tcaataaact ttctctgtca
     2581 tcctgaactt ctttattgtt ttcattttcg gagaatgttt cttcgacttg tatattgtat
     2641 ttttgggcga gataacgcag tgcctccgga aaagtcattt tctcatgttc catcacaaag
     2701 ttgacagaat cgccaccttt gccacaacca aaacatttat aaatattttt attcggatta
     2761 acggcgaaag acggagtttt ttcctgatga aaaggacaac aagctttaaa tatagaaccg
     2821 acttttttga gtggaacaaa atcgcgcacc acgtcctcta tttggacggt atcgtaaatt
     2881 cgttgtatgg tatcgcgcgt aatcaaagtt taagaagcgt gttaattagt taattagttt
     2941 aggagtttat taatttaaga gttaattagt ttgacagttt gttaagtatt caagtatttc
     3001 ttagtttatt agagagccac gtagtggcgc gaccttttta gccaataaaa acaattgtaa
     3061 acaagccacg tagtggcgac acctttattg gagattggtt tattaattta atagttaatt
     3121 agtttaagag tttttgaatt aataatttaa aatttagaat ctaaaatcta ttatcttggc
     3181 tcttgattct atgctttata tatccatatt atcttgtctg ccgtctataa tgcgaataat
     3241 ttttatctgt tcaatttctg gtttatatat tatcaaataa taacgtactt atactcgtct
     3301 gaattctgct tggtttatca ttctgccaaa attggggtgc gttttcaata tttgaattgc
     3361 ctcaaaaatt tcaaccgtta acttttccgt aaatactatg gatttgttgc ggttaaacca
     3421 atattccaaa atttgcatta aatcatcttc tgctttttgt gaccaaacta tcttttttga
     3481 tttatccatg ctttcacctt agaattcaat aattcattat caatataccg gccttcatct
     3541 atggctgtat ctgcaacata aaacgaagca atttcttcat ccgaaaagac atattcatca
     3601 gcctttaaaa tgcgaaacac ctcagccaat aattgcacat catctgagtt ttgaattttt
     3661 aaaataatat cgtcttttat agtcatgtat taaagttagt aaaaattttg tgtttagagg
     3721 ttggaagtca agagttaatc agcttaggag tatattaatt taatagttaa ttagtttgta
     3781 atttaaaaat ctaaaattta gtatctaaaa tctcttgtct tggctcttga ttcttgtatc
     3841 taaaatctca ttactcaata cccactacta tatcttgatt cttggttctc gtatctattc
     3901 atcatcaact acaaataact agcctctcag ttctgcataa tacttataaa aataaggtat
     3961 cgtttcaatg cctttaaaat aattaaacaa accataatgt tcattgggtg agtgtatatt
     4021 atcactgtct aaaccaaatc ccatcaaaac agatttcaag cctaaaatat cttcaaacaa
     4081 ggctacaatt ggaatacttg caccgtttcg catcgccaaa ggttttttgc caaaggtatg
     4141 ttccagtgcc ttgtaagcag ccacaaattc tacagaatct accggcgtta cataaggctc
     4201 accgccatgg tgttcggtta ctttcacagt cacggtttcc ggagctattt tttcaaaata
     4261 cttggtaaac aactcagcaa ttttctttga agattgattg ggtactaaac gcatggaaat
     4321 ttttgcagat gctttagcag gcaatactgt ttttgctcct tcgccgatat agccgcccca
     4381 aataccattg acatccaatg taggacgaat agtgagtt
//
LOCUS       DAOQII010000218         4415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11876_length_4415_cov_7.230734, whole genome shotgun sequence.
ACCESSION   DAOQII010000218 DAOQII010000000
VERSION     DAOQII010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11876_length_4415_cov_7.230734"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..659)
                     /locus_tag="PLF48_09635"
     CDS             complement(51..659)
                     /locus_tag="PLF48_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF49629.1"
                     /translation="MGRKSTDKKRNTNELKREKYLNLLTQTFKVHGLRKYTMDSIAKE
                     LNITKATLYQYFKSKDEMVEIILERVIFQLRDFEKIAQNQNLSFEERYYQIIELFAGV
                     ISDISNIFLEDLRNDYPKLWKDVHNFIEYTSEVLFVFYDSGKKAGVFKDINSAVLAMT
                     DRMFFNAVSDVEFLNKNKLTLKQAFEEYYKVKCFGFIHAGKS"
     gene            776..1870
                     /locus_tag="PLF48_09640"
     CDS             776..1870
                     /locus_tag="PLF48_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNF49630.1"
                     /translation="MKKLWREGKYVLPIIVLPLIVLISFYGRGFWTFFAGIFAFGFIP
                     VMDLIFEGNTDNLSEEEEKAERGKRYYDYLLYITVPLQFGLLGIYLYRISHVDLQWYE
                     YIGMTWAALLLCGVIGINIGHELGHRTKKYEQFMAKSLLMTSLYMHFFIEHNRGHHKN
                     VASPLDPATSKINEPLYLFYFRSVFGGWMSAWHLEKRRLEKQNLSFWNPVHNEMLRFQ
                     LIQFALCIGIFLAFGWIGLLGFLASATGGFLLLETVNYIEHYGMLRHEISPGRFERVR
                     PQHSWNSNHELGRIFLFELTRHSDHHYNAGRKYQILRHFDNAPQMPTGYPGMMIISLI
                     PPLWFSVMNPRVQNHLALLQTMPTETTVAV"
     gene            complement(1885..2481)
                     /locus_tag="PLF48_09645"
     CDS             complement(1885..2481)
                     /locus_tag="PLF48_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HNF49631.1"
                     /translation="MHTSLQQNVFFVKEHVGLFKVASNYDVYHPESRELLIECREPNI
                     GFFTKLFRILGYKVMTPFNIEVRATDGTLITRITRGTVFFRSVVEVFDEKEQLIGKFR
                     QRMLSLGGKFDVLDANDQVICTLKGKWASWEFSFMKDNAELAKVTKKWAGLGKEMFTT
                     ADNYALEINPAVGKDSQLRTLILAAVLCIDMVLKENNG"
     gene            complement(2573..3478)
                     /locus_tag="PLF48_09650"
     CDS             complement(2573..3478)
                     /locus_tag="PLF48_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNF49632.1"
                     /translation="MKKIRKIYEIIRDAYRYYRHDSVSKFCAALSYYTLFALPPLLLL
                     LLKITGFFINPADIETELLKDLTELFGKSTATEINNVIQQMNVFNSNGWITLFSTITL
                     IFGASGIFSEIQSTLHIIWEPIYMQQRNWKSTLLNRMFSLLMIILCSALVLVFMLLNT
                     VLNFAHQYLTVLLKSSTVTILSLTNSSLIFMMTTILFLFIYRILPSVKLKWKYIITAS
                     LSASVLFLIGKYIIGLYLAKMKIISIYGAAGTIIVLLVWVYYMSALLYFGAEVAKAYY
                     LADKVKTDKEAEKKLASVYENTEHQ"
     gene            complement(3505..>4415)
                     /locus_tag="PLF48_09655"
     CDS             complement(3505..>4415)
                     /locus_tag="PLF48_09655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF49633.1"
                     /translation="INDMEKIVQCVVCNHTDFRIYTEAKDNYSNESFTVQQCNACGFV
                     FTNPRPEEAAIGKYYNMPDYMSHTSHSKGMVQSVYRFARNYMKKRKLKLIQNLIQKEN
                     QFDLLDYGCGTGDFLSFMKQNKINTIGIEPDEMARKVAHEVNQIQVYALDELKKIEEN
                     KFDVITLWHVLEHIYPVHEQLDFFKLWLRNKGKLIIAVPNIESYDSAYYGKYWDALDV
                     PRHIYHFSPSTVQKLVEQHGFALTQMTPLPLDAYYVSMRSEWHKGTPKWLSFPKAVIR
                     GFLSNRSAKKDGNYSSLIYVFEKLQG"
BASE COUNT         1332 a          815 c          784 g         1484 t
ORIGIN      
        1 ctgaaaccaa atgaaaactt tgtagcgagt tttagtattc gctttttaag ttaactctta
       61 cctgcgtgga taaacccaaa acattttact ttgtaatatt cttcaaaagc ctgttttaat
      121 gtaagtttgt ttttatttaa aaattcaacg tcagaaacag cattgaaaaa catcctgtcg
      181 gtcatggcta acacggcaga attaatgtct ttgaatacgc ctgctttttt tcccgaatca
      241 taaaaaacaa ataagacttc agaagtgtat tcaataaaat tatgaacatc cttccataat
      301 tttggataat cgttacgcaa atcttccaaa aaaatattgg agatatcaga aattactcct
      361 gcaaatagtt caataatctg ataatagcgt tcttcaaaag acagattttg gttttgggca
      421 attttttcaa aatcacgcaa ctgaaaaatg acacgttcta aaataatttc caccatttca
      481 tctttcgatt taaaatattg atacaaagta gcctttgtta tatttaattc tttggcaata
      541 ctatccattg tgtactttcg caaaccatgc accttaaatg tctgtgtaag cagattcagg
      601 tatttttccc gctttagttc atttgtattg cgttttttat ctgtggattt tcttcccatt
      661 tttcaaaaat aacaagtttt ttttacgctt cttcggatta acaaaagata attcttttac
      721 taaattaaac tatttattgt aaatagttta attttataca tctaaattta taggtatgaa
      781 aaaactatgg cgagaaggca agtatgtttt accaataatt gtattgccac ttattgtttt
      841 aatttcattt tacgggagag ggttctggac tttctttgcc ggtatatttg cctttggttt
      901 tattccggta atggacttga tatttgaagg aaatactgat aatttaagcg aagaagaaga
      961 aaaagctgaa cgaggtaagc gttactatga ttatctcctt tatatcactg tgcctttgca
     1021 attcggctta ttggggatat atttgtatcg catttctcat gttgatttgc aatggtatga
     1081 atatatcggg atgacttggg cggcgttgtt attgtgtggc gttatcggaa taaatattgg
     1141 acatgaactt ggacacagaa ctaaaaaata tgagcaattt atggcaaagt cattactgat
     1201 gacttcactg tatatgcatt tttttatcga gcacaaccgc gggcatcaca aaaatgtagc
     1261 ctctccttta gaccctgcaa catcaaaaat caacgaacct ttgtatttgt tttattttcg
     1321 cagtgttttc ggtgggtgga tgagtgcctg gcacttggaa aaacgcagac tggaaaaaca
     1381 aaatttgtct ttctggaatc cggtacataa tgagatgctg cgctttcagc tgattcagtt
     1441 tgcgctctgc atcggtatct ttttagcttt cggctggata ggcttgctag ggtttttggc
     1501 atctgccacc ggaggatttt tattgctcga aacagttaat tatatcgaac attatggtat
     1561 gctccgtcac gaaatttctc ccggaagatt tgagcgtgtt cgtccgcagc atagctggaa
     1621 ctcaaatcac gaactgggaa gaattttttt atttgagttg actcgccaca gcgaccatca
     1681 ttataatgca ggaagaaaat accagatttt gcgtcatttt gataatgctc cgcaaatgcc
     1741 caccggatat ccgggtatga tgatcatctc cttaattccg ccattgtggt ttagtgtgat
     1801 gaatcctcgt gtgcaaaacc atttagccct gctgcaaact atgccaacag agacaacagt
     1861 tgctgtttag tttagtttat ttttctatcc attgttttct ttcagcacca tatcaataca
     1921 taacacggct gcaagaataa gtgtgcgcaa ttggctgtcc ttgcctacag caggattaat
     1981 ttccagtgcg taattatcgg cggtggtaaa catttctttt cctaaacctg cccatttttt
     2041 ggttacttta gccaactccg cattatcttt cataaaagaa aattcccagc ttgcccattt
     2101 accttttagt gtgcatatta cctggtcgtt tgcatcaagt acatcaaact tgccgcccag
     2161 acttagcatg cgctgtctga attttccgat aagttgttct ttttcatcaa atacttctac
     2221 cacagaccta aaaaacacag tgcctcttgt tatacgggta atcagcgtgc catcggtagc
     2281 tctgacttca atattaaaag gggtcattac tttataaccc aatattctga ataatttggt
     2341 aaagaaaccg atattcggtt cgcggcattc tataagcaat tcccttgatt cgggatggta
     2401 aacatcgtag ttgctggcta ctttaaataa cccgacatgt tctttgacga aaaacacatt
     2461 ttgctgaagt gaggtatgca tagatttaaa ttagtcactt ttcttttacg aaataataaa
     2521 atataacaac tgatttgctt tataagttcc atcagaattt actgaatgaa aattattgat
     2581 gttcagtgtt ttcatatact gaagctaatt tcttttctgc ttctttgtcc gtctttactt
     2641 tatctgccag ataatatgct ttggccactt cagcaccgaa atacaacaat gcagacatgt
     2701 aatataccca aaccagcaaa acaatgatgg ttccggctgc gccgtaaatg ctgataattt
     2761 tcattttagc gaggtatagt cctattatat acttaccaat caaaaacaat acggatgcac
     2821 taagggatgc agtaattatg tatttccatt ttagttttac cgaaggcaat atccgataaa
     2881 taaataaaaa caagatggtc gtcatcataa aaataagact ggagttggta agcgataata
     2941 tcgttacggt tgatgatttt aataagacag taagatactg atgtgcgaaa ttgagtactg
     3001 tatttaataa cataaatact aaaactaatg ctgaacatag tatgatcatc aataatgaaa
     3061 acattcgatt gagcaatgtc gatttccagt ttcgttgttg catataaatg ggttcccaaa
     3121 taatatgcaa agtgctttgt atctcagaaa aaatgccgga tgcaccaaaa ataagtgtaa
     3181 tggtgctgaa cagggtaatc catccgtttg aattaaatac attcatttgt tgaattacat
     3241 tattgatttc tgtggcagta gatttgccga acaactctgt caaatctttc agtagctccg
     3301 tttctatatc agcaggatta ataaagaaac cggttatctt gagtagtaaa agcaatagtg
     3361 gaggcaatgc aaataaagta taataagaaa gtgctgcaca aaatttcgat accgaatcat
     3421 gccggtaata acggtaagca tctctgatta tctcatatat tttccggatt ttcttcattt
     3481 gggatatccg ctttttaaac tagactaacc ctgtagtttc tcaaaaacat aaatcagact
     3541 actgtagttg ccgtcttttt tagcagagcg attactcaaa aaacctctaa ttacggcttt
     3601 ggggaatgac aaccacttgg gtgttccttt atgccattca cttcgcatcg aaacataata
     3661 ggcatcaagt ggcagcggtg tcatttgtgt taacgcaaac ccatgttgtt ccactagttt
     3721 ctgcaccgta gaaggtgaaa aatgataaat atgtcggggt acatccaagg catcccaata
     3781 cttgccataa taagccgaat cataactttc gatatttgga actgcgatga tgagtttacc
     3841 tttgtttcgt aaccaaagtt taaagaaatc cagttgctca tgcaccggat agatatgttc
     3901 caatacatgc cacaacgtaa tgacatcaaa tttattttct tctatttttt ttaactcatc
     3961 aagggcatat acttgtatct gattaacctc atgggctact tttctggcca tctcatccgg
     4021 ctctatgcca atggtattta tcttgttttg cttcatgaaa gaaagaaaat ctccggtgcc
     4081 gcaaccataa tccaacaagt caaattgatt ttctttttga ataaggtttt ggataagctt
     4141 tagctttctt tttttcatgt aattgcgcgc aaaccgataa acagactgta ccatgccttt
     4201 agaatggctg gtatgcgaca tgtaatctgg catattatag tactttccaa tggctgcttc
     4261 ttccgggcgg ggattagtaa aaacaaatcc gcaagcgttg cattgctgaa cggtaaagct
     4321 ttcgttagaa taattgtcct tggcttcggt ataaattctg aaatcggtat ggttgcaaac
     4381 cacacactgc actatttttt ccatatcatt aatta
//
LOCUS       DAOQII010000219         4406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_11917_length_4406_cov_5.004367, whole genome shotgun sequence.
ACCESSION   DAOQII010000219 DAOQII010000000
VERSION     DAOQII010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11917_length_4406_cov_5.004367"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="PLF48_09660"
     CDS             <1..1876
                     /locus_tag="PLF48_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49634.1"
                     /translation="DELKKQQQVAQQQKPLPTVVTQTPSSQQINQQPKSNNTPVEPTT
                     YKQQQQVLTQEVDPTTFSSPAPVYNAPLKKADPVQDDLQAKLDELKKQQQNNKINAST
                     NTTDYNKPYDPNANTNYYPNPKPVTPTTNANTEALDQKLAELKQQQYQPQQSTRINDP
                     NATKKYDVTTVKERVPDKATEEYDDFQKKLDELKNQQKNPTVKTTTAPANTTPPVSNP
                     VASANPTHTVSTPVASNTPDTKPETPKTSKVSNPDLSKYEEKLKKLKEEMAAMGMPEK
                     PVEATPATATIQPKTTTAENTPTKPLDNYSTPTDELQKKLNEFKQEQTTSPITNNITP
                     DKTDAKISTPVMEAPLPVAQKTNYQLELEKAKLDSLQQAQKNAEKQLADMLDKMGNNK
                     LSLEKDITDLKDKVAQFEKDKNNLTQQNTQLVDEKKKLEDQKKQMDDMLALMQTERDR
                     LAMEKVKMEQDKKLLDQLKKQQELEVFAMKRAIDSLAKIKAKAQYRADSILNLDPAKS
                     PTLEVGTIIIADKILFMADASYLAINSYPEVDKIAAFLQKNKTLKVEIGGHTNGLCDE
                     AFCNNLSTNRAKTIVDYLVSKGIDRNRLTYKGYGKQFLVAPAGSPMNQRVEIKILAIK
                     "
     gene            1901..2794
                     /locus_tag="PLF48_09665"
     CDS             1901..2794
                     /locus_tag="PLF48_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF49635.1"
                     /translation="MNVLQQTMATFPDISEEVWKQKIEKDLKGKSFNDLILHSAEGIE
                     VMPFYTKETISNQRLLTTPFRNGDCKNAVYYAVNNPAETNNQALRDLQYGIDALLFFA
                     DDISINEKNGISENIDKSAVELIYLNTENCISIPHQHSCTDALVVAIKKLTEADIFAD
                     KTMYFKFSSSQNYFFEIAKYRAFRWLIARIQEVQHLPFDYRIIVQLETGKTDDDFIYN
                     EILRNTTATMAAVLGGADYVITHPYILNDENAKRIAINTMHILKYESHFTKLPDTGKG
                     SYYIEYLSYHMAKNVWDKFQQ"
     gene            2822..3211
                     /locus_tag="PLF48_09670"
     CDS             2822..3211
                     /locus_tag="PLF48_09670"
                     /inference="COORDINATES: protein motif:HMM:NF018826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HNF49636.1"
                     /translation="MFTIEQIEKAHDKVKTGAAFPEFIASIKAMGVIAFETWVVDRST
                     EYIGENNYKVNSLPQGEMLKVANSSNAENFMVCLNRHQHGETDFMTFCKDCAENGIER
                     WKVSLSDMTCTYYDTSNNIVLTEKIPG"
     gene            complement(3262..3624)
                     /locus_tag="PLF48_09675"
     CDS             complement(3262..3624)
                     /locus_tag="PLF48_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HNF49637.1"
                     /translation="MTDIKFYISAISKVVELIGVITIFIGILYSLAIFIARINKSNSV
                     SFDNLRQTLGKSILLGLEILIAADIMATVATDPTLQSVIVLGVIVLIRTLLSFSLQIE
                     IDGKLPWQKAETAQSKKE"
     gene            complement(3755..>4406)
                     /locus_tag="PLF48_09680"
     CDS             complement(3755..>4406)
                     /locus_tag="PLF48_09680"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49638.1"
                     /translation="GKIARNANKSTVTASIMSNGKEIARFDGKNVVNISSSQIVNNNI
                     AIKTSGSGKLYYFWETEGLPAQADNKQEDNFLEVRRTYKDRYGKVFNNYFNQNDLVVV
                     EISIRSTNGIEVPNVAITDLLPAGFEIENARITDIPQLEWMKQGATPDYKDIRDDRMN
                     IMVTATPYIQKYYYLCRAVSLGMFNVGPVAADAMYRGEYHSYHGAGKISIGRKTVM"
BASE COUNT         1613 a          847 c          760 g         1186 t
ORIGIN      
        1 agatgagttg aaaaagcaac aacaagtagc gcaacaacaa aaaccactac caacagttgt
       61 tacacaaact ccatcttcac aacaaatcaa tcaacaacct aaatcgaata acacacctgt
      121 tgaaccaaca acttacaaac aacagcagca agtactgaca caagaggttg acccaacaac
      181 tttcagctct ccggctcctg tatataatgc tccacttaaa aaagcagatc cggtacaaga
      241 tgatttacag gcaaaattag atgagctaaa aaaacaacaa caaaacaaca agataaacgc
      301 atcaacaaac accacagatt ataacaagcc ttatgacccg aatgccaata ccaattatta
      361 tcccaacccc aaaccggtta cgcctactac aaatgccaat acagaagcct tagatcaaaa
      421 actagccgaa ttaaaacaac aacaatatca gccacaacaa agtacacgca tcaatgatcc
      481 gaatgcgacg aaaaaatatg acgtaacaac tgtaaaagaa agagtgccgg ataaagcaac
      541 agaagaatat gatgattttc agaaaaaact ggatgaactg aaaaaccagc aaaaaaatcc
      601 aactgtcaaa acaactacgg ctcctgcaaa tacgacacca cctgtttcta atcctgtagc
      661 aagtgcaaac cctactcata ccgtatcgac tcctgttgca tcaaacacac ctgatacgaa
      721 gccggaaact ccgaaaactt caaaagtttc caatcctgat ttaagcaaat atgaagaaaa
      781 attaaaaaag ctgaaagaag aaatggctgc gatgggcatg cctgaaaagc ctgtggaggc
      841 aactcctgct actgcaacta ttcagccaaa gactacaact gcagaaaaca caccaacaaa
      901 accattggat aattactcca ctcctaccga tgagttgcaa aaaaaattaa acgaatttaa
      961 acaagaacaa actacaagcc ctattacaaa taatattact cctgataaaa cagatgctaa
     1021 aatttctacc ccggtaatgg aagccccact gcctgttgcg caaaaaacaa actatcaact
     1081 ggaactggaa aaagcaaaat tagattcact gcaacaagcg caaaaaaatg ccgaaaaaca
     1141 attggctgat atgctggata aaatgggcaa caataagctt tctcttgaaa aagacattac
     1201 agatctaaaa gataaagtgg cacaatttga aaaagacaaa aacaacttga cacaacaaaa
     1261 tacacaattg gtagacgaga agaaaaaact ggaagatcag aaaaaacaaa tggacgatat
     1321 gctggccttg atgcaaacgg aacgtgaccg attagccatg gaaaaagtga aaatggaaca
     1381 ggataaaaag ctattggatc aattgaaaaa acaacaagag cttgaagtat ttgccatgaa
     1441 gcgagctata gattctttgg caaaaataaa ggcaaaagcg cagtaccgtg ccgattctat
     1501 attgaattta gatcctgcga aaagccctac tttggaggta ggcacaatca ttattgctga
     1561 taaaatattg tttatggcag atgcttctta tttggctata aattcatatc ctgaagtaga
     1621 taaaattgct gctttcttac aaaaaaacaa aaccttaaaa gtggaaatcg gcggacatac
     1681 caacggactt tgcgatgagg cattttgcaa caatttatct actaaccgag ccaaaacaat
     1741 tgttgattat ctggtttcaa aaggaataga cagaaaccgg cttacctaca agggctatgg
     1801 caaacaattc ttggttgctc ctgccggatc gccaatgaac cagcgggtag agataaaaat
     1861 attagcaata aaatagtact tccattcgta tctttgcaaa atgaatgtac tacagcagac
     1921 aatggctact tttcctgata tctcagaaga agtatggaag caaaagattg aaaaggattt
     1981 aaaggggaaa tcctttaatg atttgatatt gcatagtgca gaaggaattg aagtaatgcc
     2041 tttttacacc aaagagacca tttccaatca acggctgctc accactccat ttagaaatgg
     2101 agattgcaaa aatgctgttt attatgcggt aaataatccg gcagaaacca acaaccaagc
     2161 tctacgggat ttgcaatatg gtatagatgc cttattgttt tttgctgatg atatttcaat
     2221 aaatgaaaaa aatgggataa gcgaaaatat tgataaaagt gctgttgaac ttatttattt
     2281 gaatacagaa aattgcataa gcattccaca tcaacatagc tgtactgatg ctttagttgt
     2341 tgcaataaaa aaacttactg aagctgatat ttttgcagat aaaacgatgt atttcaaatt
     2401 ttcgagtagt caaaattact tttttgaaat cgctaaatac agggcattca gatggctgat
     2461 tgcacgcata caggaagttc aacaccttcc atttgattat cggataattg ttcaattaga
     2521 gaccggaaag accgatgatg attttatcta taatgaaata ttgcgcaata ccactgcaac
     2581 gatggcggca gtactcggtg gagcagacta tgtcattaca catccttata ttttaaatga
     2641 tgaaaatgcg aagcgcattg ccatcaacac gatgcatata ttaaaatacg aatcgcattt
     2701 caccaaactt cccgataccg gaaaaggctc atactacatc gaatacttat cttatcacat
     2761 ggcaaaaaat gtctgggata aatttcaaca ataagcaaag ataaatcata aaaaagttgg
     2821 catgtttaca atagaacaaa ttgaaaaagc acatgataaa gttaagaccg gtgctgcctt
     2881 tcctgaattt attgcatcaa tcaaagcaat gggcgtgatt gcgtttgaaa cttgggtagt
     2941 tgacagaagt actgaataca ttggagaaaa taactataaa gttaattcac tcccgcaagg
     3001 cgaaatgctg aaagttgcca attcaagcaa tgcagagaat tttatggtat gtctaaatcg
     3061 gcatcaacat ggtgaaacag actttatgac attttgcaag gattgtgctg aaaatggaat
     3121 tgagcgttgg aaagtcagtt tatcagatat gacctgtaca tactatgata catcaaacaa
     3181 cattgtgttg acagaaaaaa ttccgggata aatacccagt actgatacga aaatataaac
     3241 ccggtaaatc ttctgatttg tttattcctt tttgctttga gcagtttctg ctttttgcca
     3301 aggtaatttt ccgtctattt ctatttgcag agaaaaactg agtaatgttc ggattaacac
     3361 tattacaccg agtacaataa cggactgtaa tgtggggtcg gttgcaacgg ttgccatgat
     3421 atctgctgct attaaaattt caagacctaa taaaatagat ttacctaaag tctgtcgtaa
     3481 attatcaaaa ctgacactat tgcttttatt aattctggca atgaaaatag ctaatgaata
     3541 gagaatgcct ataaaaatgg taatcacacc aatgagttcc accacttttg aaattgcact
     3601 tatataaaat ttaatgtctg tcacgctgta taaatttatt acaggagtaa gatataaaat
     3661 taatacgttt atttaaagta ttaaataact ctggttattt ataaaaaaac ccttattaca
     3721 ataatttgta acaagggttt taatctactt tgcattacat gacggttttt ctgcctatac
     3781 taatttttcc ggcaccgtga taagaatgat actccccgcg atacatggca tcagcggcaa
     3841 cgggacccac attgaacata cccaatgata cggctctgca tagataataa tatttctgga
     3901 tatacggtgt agccgttacc atgatattca ttctgtcatc acgaatgtct ttgtagtctg
     3961 gtgtagctcc ttgtttcatc cactccagtt gagggatatc tgtgatacgg gcattttcaa
     4021 tttcaaagcc ggccggtaat aaatcggtaa tggcaacatt aggcacttca atgccatttg
     4081 tactgcgaat actgatttct acaaccacca aatcattctg attaaaataa ttattaaaca
     4141 cttttccata gcggtctttg tatgttctgc gtacttccaa gaagttgtct tcctgtttgt
     4201 tatcagcctg tgcaggcagt ccttctgttt cccaaaaata gtagagcttg ccgcttccgc
     4261 tagtttttat tgcaatattg ttatttacaa tttgtgaaga tgaaatattg acaacatttt
     4321 taccatcaaa cctggctatt tcttttccgt tcgacataat acttgccgtt acggtagatt
     4381 tattggcatt tctggcaatt ttaccc
//
LOCUS       DAOQII010000220         4343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12184_length_4343_cov_5.547108, whole genome shotgun sequence.
ACCESSION   DAOQII010000220 DAOQII010000000
VERSION     DAOQII010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12184_length_4343_cov_5.547108"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLF48_09685"
     CDS             <1..393
                     /locus_tag="PLF48_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF49639.1"
                     /translation="HTKLKVHGDEHTYFVFADEKRIMQVLENLISNSIKYGKEGGKTD
                     IYIHDLDEQYLIEIADDGPGIPQQHLARVFERFYRADKSRSRKVGGTGLGLSIVKNII
                     EAHEQTINVSSEEGKGTTFNFTIQKYIE"
     gene            complement(427..1251)
                     /locus_tag="PLF48_09690"
     CDS             complement(427..1251)
                     /locus_tag="PLF48_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF49640.1"
                     /translation="MNTILIPVDFSETSLKAISYAEIIAERLGAEMHLCHMYSPMMYS
                     SVYGADFVFTESVFKDMEENARQQFVPIEEALAKKNIKVKTHVIFGNLTEDLNRIAKE
                     LKCELLVAGTTGSSTVLNKIFGSNASYILENCECPVILVPKDYEQFEFNHIVFADDFS
                     KDELPVIDKALEFAHVMGVNKLDLLKVDTHSFNVFNDDSKIEKIINTYSQKEVKLNFV
                     LAGSFEKGFEDYCKEHSTDLLIMSTEKKSFIQQLFSHSNTHYMAMHTKVPMMVFHV"
     gene            complement(1341..2363)
                     /gene="murB"
                     /locus_tag="PLF48_09695"
     CDS             complement(1341..2363)
                     /gene="murB"
                     /locus_tag="PLF48_09695"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNF49641.1"
                     /translation="MLSFYQNYSLKKYNSFGLDIRAKLFVSIKSLEDFLLLLKTSEYL
                     ENKHLILGGGSNILFSSHYDGLVIKNEIVGIRILKEDEKDIWLTAFSGTNWHSFVMYG
                     VEHSYGGLENLSLIPGTVGAAPIQNIGAYGVEMKDCFEELEAIELATGDLYKFDKELC
                     AFGYRDSIFKREAKGQYFIYSVTFKLSKHPQVNTKYGDIQKTLQEENKISPTVKDVSD
                     AVVAIRKSKLPDPNEMGNAGSFFKNPELSQQQFELLFQQYPEIPHYHLPENKIKIPAA
                     WLIEQCGWKGKKSGNTGNHAKQALVIVNYGNASGLEIWQHAQHVQQSVLDKFGITLEA
                     EVNIVG"
     gene            2658..3022
                     /gene="rnpB"
                     /locus_tag="PLF48_09700"
     ncRNA           2658..3022
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLF48_09700"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1284 a          840 c          837 g         1382 t
ORIGIN      
        1 catactaaac tgaaagttca tggtgatgag catacttatt tcgtttttgc cgatgaaaaa
       61 cgcatcatgc aggtgttgga aaatcttatt tcgaactcaa taaaatatgg caaagagggt
      121 ggcaaaactg atatttacat tcacgatctg gatgaacaat atctgataga aattgcagat
      181 gacggaccgg gcataccgca acagcatctg gcaagggtct tcgagcgttt ctatcgggca
      241 gataagagcc gcagccgaaa ggttggaggt acagggttgg gtctatctat cgtaaaaaat
      301 attatagagg cacacgaaca aaccatcaac gtgagcagcg aagaaggcaa aggcacgacg
      361 tttaatttca ctattcaaaa gtatattgaa taaatagccg gcagactcta tcaatatcag
      421 attcacttat acatgaaaga ccatcatcgg cactttggta tgcatggcca tataatgtgt
      481 attgctatga gaaaagagtt gttgtataaa tgattttttc tctgtagaca taattaataa
      541 atctgtactg tgctctttgc aatagtcttc aaagcctttc tcgaacgatc ccgccaaaac
      601 aaaattcaat ttcacttctt tttggctgta agtattgatt attttctcaa ttttagagtc
      661 gtcattaaac acattaaaac tatgcgtatc tactttcagt aaatctaatt tattaacgcc
      721 catcacatgt gcaaattcaa gggctttgtc aataaccgga agctcatctt tactgaaatc
      781 atcagcaaat acgatatggt taaattcgaa ctgctcatag tctttaggca ctaaaataac
      841 aggacattca caattttcca ggatatatga tgcattgctg ccaaatattt tattaagcac
      901 tgtgctgcta cctgtagtac cggctaccaa caattcacat ttcagttctt tggcaatgcg
      961 gtttaaatct tccgtcaggt ttccaaaaat gacatgagtt tttactttaa tatttttttt
     1021 ggcaagcgcc tcttcaattg gtacaaattg ctgacgggca ttctcttcca tatctttaaa
     1081 aacggactcg gtaaaaacaa aatctgcacc ataaacggag ctgtacatca ttggagaata
     1141 catgtgacac aaatgcattt ctgctcctaa gcgctctgca ataatttctg cataactgat
     1201 tgccttgaga gaagtttcag aaaaatcaac cggaataaga atagtattca tggaaatatt
     1261 ttgcttaaag ttaataagat attcgaatgg tacgtaatga tttttatcag ggtagggaaa
     1321 aaattagcat caattaataa ctaacccact atattgactt ctgcttccag tgtaatgccg
     1381 aatttatcta aaacggattg ttgtacatgt tgtgcgtgct gccagatttc caaaccactg
     1441 gcgtttccat aatttacaat gaccaaagcc tgcttggcgt gattgcccgt attgcccgat
     1501 tttttccctt tccacccaca ttgttctatc agccatgccg ccggaatttt tattttgttt
     1561 tcgggcaaat gataatgcgg tatttccgga tattgctgaa aaagtagctc aaattgctgt
     1621 tgggatagtt ccggattttt aaagaaactg cccgcattgc ccatttcatt tgggtcgggt
     1681 aatttacttt ttctgatggc aaccactgca tccgatacat cttttaccgt tggacttatt
     1741 ttgttttctt cttgcaatgt tttctggatg tcgccgtact tggtatttac ttgtgggtgt
     1801 ttggataatt tgaaagtaac cgaataaata aaatattgtc ctttagcttc tcgtttaaaa
     1861 atactgtcgc ggtagccaaa agcgcatagc tctttatcaa atttatacaa atcgcccgtc
     1921 gctaattcaa tggcttccag ttcttcaaaa cagtctttca tttctacccc gtaagcaccg
     1981 atattttgta tgggtgcagc acccaccgta ccgggtatca acgataaatt ttccaatcca
     2041 ccataactat gttctacacc atacatcacg aaactatgcc agttggtgcc cgaaaatgca
     2101 gtaagccaaa tatctttctc atcctctttt aatatgcgga taccaactat ctcattctta
     2161 atcactaatc cgtcataatg gctgctaaat aaaatattgc tgcctccgcc aagaatcagg
     2221 tgtttgtttt cgaggtattc ggacgttttt aaaagtaaaa gaaaatcctc cagagattta
     2281 atgcttacga agagtttggc tcttatatcc aacccaaagg agttgtattt tttgagtgaa
     2341 taattttgat agaaagatag catataaggc aataaatata agcgtaaaat cagacatcag
     2401 ataatgacac caaatatttt tgtacgctat ttcaagttaa tttttaatgc cactcatcta
     2461 atttgatatc ctgcgtttgg gttaagaaaa ataaggggat aaaatagttg atttcacaca
     2521 tttttctcca acttaacatg taggaggaat ttaaaggagg tggtttttga taaagaaatc
     2581 aactattttt ttacattgaa acacttatcc cgtacttagg ttgatagttc acctgtcaaa
     2641 aaatagctaa atttgcagtg gcaggttgcg ctatcgctcc gagcaatcgg ggaggaaagt
     2701 ccgggcagca cagagcagtt tgacggataa cttccgtaag ttgaaagact tggaaagtgc
     2761 cacagaaaat ggtctcgttt tttagcgaga gtgaaaaagt gcttttgcag caatgcataa
     2821 gacgggcaaa ccccaaacgc tgaaacacca tgtaaaccaa cgctgatata gggtggctcg
     2881 cctaatgttg gagggtaggt ggatagatct cgtcagcaat ggcgagacca gataaatgat
     2941 agtggctgca gttgagaata ggcgcataag tgtccaattt cagcaagcag tacagaatcc
     3001 ggcttacaga cctgccgttt tttttatggt tactttttcc aaggccttaa attcctgata
     3061 atagcctttt atgataaaat cgggatcctc tacaatatgt tcgtccgtta caatgcctaa
     3121 gcatacacgg ttgttatagc tgataatact gataccaata cccaaagggc ctgtttgcgg
     3181 cacccaaaat acgatatcat ttatttgttg ccctccgata taaatcggat ggttgggacc
     3241 cggcacattg gtaatcacgc cggtaatttg tgtgcctaaa taggtggcta aaatgtcttc
     3301 aattttagta ggtaaaacat cagccataat attcattaga ttgtatatca aaaaaggctc
     3361 gaaagaatgt tttagcaatt ctgttttggc atgtatatat gcaatgcgtt tttcgatttg
     3421 gcgtacatgc acgggaagtt ctattgacaa catcccgatt ttattgtgta cttttatttg
     3481 ttcgtccttt ttgcggatat ttaccggaac tacaatacgg ataggctcac tcagcttttg
     3541 tttatgtctt agcaaatgcc tgcgtatggc tccggttatc aaggcaagta aaatatcatt
     3601 tactgttacc ctgtatattt ttcctatggc tttgatttca tctaaaggca aagcctcaga
     3661 ccatgctgct tttttgcgaa acgaaagctc gcctttatac aggctgtttt ttactgtttt
     3721 gccgaataat agtttgatta cttctttaga aacatgagca gtctcggtga gggtttcttc
     3781 aattttgttt ctgtttttaa aaaaatcttc cgcttggtga taccactctt ttcctgtttg
     3841 caacaggtga gaaaattcat ccataacggt atggctgtgt atcacatcag gcttgacatc
     3901 aatagcagga ggtggcagca gagaagctgt ttttgtttta gcggttaatg aaaacaatac
     3961 ttttatcaaa gcaatgccgt ctgctatagc atgatgaatg cgccaaagca atacacttcc
     4021 gccattataa ttgtctataa gatgcacttg ccacaaggct tttccttcgg gcaacggttc
     4081 gctgatcagc tcactgatga tttgctgaag accaatgtat tttgttttat cgggtaaaac
     4141 tatgcgctga atatgcacat cgatatcaaa attattgtca gcacgccagt aaggtttccc
     4201 cagcttatga acaacttttt tgctgaaacg ctgatgaatg agtaaccgtt cttgcaccac
     4261 ctttttaagc tggtcgagac ttaggggagt agctaaaacc atttttccgg tgattgttac
     4321 aaggctgttg cgtttattca tat
//
LOCUS       DAOQII010000221         4309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12327_length_4309_cov_4.401034, whole genome shotgun sequence.
ACCESSION   DAOQII010000221 DAOQII010000000
VERSION     DAOQII010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12327_length_4309_cov_4.401034"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLF48_09705"
     CDS             complement(<1..480)
                     /locus_tag="PLF48_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNF49642.1"
                     /translation="MVILLATFSWKWITVPLIGAFIGWITNWLAIKMLFRPRQPVKIL
                     WFTFQGIFPKNKPRIANKLGEIVQRDLINFTDIKDRLKDPDALKNFEEEIAQRVDVAI
                     RERIERSPILDVIVPDQLISNVHKTIVEEIGKNLPNVIDTSLTKIEQKLDIHQLVKNK
                     "
     gene            complement(484..1125)
                     /gene="pyrE"
                     /locus_tag="PLF48_09710"
     CDS             complement(484..1125)
                     /gene="pyrE"
                     /locus_tag="PLF48_09710"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNF49643.1"
                     /translation="MENIAFKEKLAEYLLQIKAIQINVQNPFTWTSGWKSPVYCDNRK
                     ILSYPDVRTFVKEGFIGLINAYYPQVECIAGVATAGIPHGAIIADALNLPFIYVRSKA
                     KEHGMGNTIEGSLLHGQQVLVIEDTISTGGSSLKAVHDLREAGADILGMLAIYKYGFA
                     STKKRFEDEGVQLKTLTNYDILINAAIKQDDISSEDMETLKLWKQDPAEWHNS"
     gene            1198..1842
                     /locus_tag="PLF48_09715"
     CDS             1198..1842
                     /locus_tag="PLF48_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF49644.1"
                     /translation="MYKFNINGKKVFIVANPTQVKQALQDDYILQAYTNKGELQNIIT
                     ILQSNDNNSNWIIFGKDPETIKEVFSSFFQPIIAAGGVVLNENHHVLLIFRRGFWDLP
                     KGKVDEGESIDEAALREIEEETGLNQLNILQPVTFGFSNQDCTYHSYVLNGVNCLKQT
                     YWFMMENNGNDLLLPQTEEDIEQAIWVHKDNLHNYYDNMYESIKDVLQVAITHF"
     gene            2035..3414
                     /locus_tag="PLF48_09720"
     CDS             2035..3414
                     /locus_tag="PLF48_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF49645.1"
                     /translation="MIYDFIVVGSGSGGGALAYYLHQGGAKVLLIEAGKFYRKNTFPK
                     TEAETSAYLYWGGGIEFAKDGRMGFLRTKMVGGSSIVYQCLMDRFDDIAFNDWKAESG
                     VDWFNSAEMDKHYNQVESDLVLHKFTENERNGNARLYTKACDQLGYKWKYLRRGQSDC
                     GAEHGNDCIGCLGGCFRDSKQSSLVAFIQKAEKTGLEISTETEIHQVAPNADTCKISG
                     IQNGNKVSFEGKKVILAGGAFGTTRMMTMSGFKQKFPVLGKKFATHPQFMWLGFQNEI
                     VDAHKLMFQSVASDDPEFRKNGFKLENVFASPISIGMLMNVTGAKHQYFMKQYRYMQC
                     IEVAVRDENVGEIQCDNKGKLTIVKPMTQQDKDRKNKGLQAIKNILTTAGAKEVFESD
                     FYFGLHLMGGCVMGTDAAKSVVSPEFNVHGYNTLYICDSSIYPNAPGINPGLTIKSLG
                     HRLSTQLLK"
     gene            3557..4063
                     /locus_tag="PLF48_09725"
     CDS             3557..4063
                     /locus_tag="PLF48_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49646.1"
                     /translation="MKNALILFFISSMLFLSCKKENDNNGDFSVLTYNVAGLPQGFNA
                     DQFPVLHMSDVGKLLNKVDIVQVQEDFCYHDRLLVQNYAHPFVTPTKGCVPNGDGLNT
                     FSIYPINDLFRQKWNACNGFDCLTPKGFSFTRIAINKKVSIHFYNVHANAGSDNADMA
                     ANETILFS"
     gene            4060..>4309
                     /locus_tag="PLF48_09730"
     CDS             4060..>4309
                     /locus_tag="PLF48_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49647.1"
                     /translation="MMRYIQTHSANMPVIVMGDFNSRYTRMGDTIGLFYDNGFKDVWI
                     EKIRNGVIPSKGASLMDCTGDKSNPECEVVDKIFYRSSA"
BASE COUNT         1412 a          765 c          844 g         1288 t
ORIGIN      
        1 tttgtttttt actaactgat gaatgtctag tttctgttca attttagtta acgacgtatc
       61 aataacattt ggtaaatttt tgccaatttc ttctacaata gttttatgca cattactgat
      121 taactgatca ggtacaataa catccaaaat agggcttcgt tcaatgcgct cccgaatggc
      181 cacatctacg cgttgggcta tttcttcttc aaagttttta agtgcatccg gatcttttag
      241 cctgtctttt atatcagtaa aattaattaa atcacgctga acaatttcgc ctaatttgtt
      301 ggcaatcctg ggcttgtttt taggaaaaat gccctgaaag gtaaaccata atatctttac
      361 aggttgtcgt ggacgaaata gcattttaat agccaaccag ttagttatcc aaccgataaa
      421 cgcaccaatt agtggtacag ttatccattt ccatgaaaag gttgccagta gtattaccat
      481 aaactaagaa ttatgccatt ctgcaggatc ttgtttccat aacttcagtg tttccatatc
      541 ttccgatgag atatcatcct gtttaattgc cgcattgatt aaaatatcgt agttcgtcag
      601 tgttttaagc tgaacgcctt catcttcaaa tcgttttttc gtagacgcaa atccatattt
      661 gtaaatggct aacataccca gaatatctgc accggcttca cgcaaatcat gcacggcttt
      721 taacgaactg ccacccgtag agatcgtatc ttcaatcact aaaacttgtt gtccgtgcag
      781 aaggctgcct tctatcgtat tgcccatacc atgctctttg gccttagaac gcacatagat
      841 aaagggtaaa ttaagcgcat ctgcaataat ggcaccatgc ggaatgccag ctgtagccac
      901 tccggcaatg cattctacct gtgggtagta ggcatttatt aatccgataa atccttcttt
      961 tacaaaagtg cgcacatcag gataagataa aattttgcgg ttatcacagt aaaccggaga
     1021 tttccaaccg ctagtccatg taaaaggatt ttgtacgttg atttgaattg cttttatttg
     1081 cagtaaatat tcagctaatt tttctttaaa ggctatattt tccataagta tttagtcagg
     1141 caagatagaa gtattttgtt aaacaaaaaa tgataaacgg cataaattaa aattcccatg
     1201 tataaattca acatcaacgg caaaaaagtg tttattgttg ccaatcccac gcaagtaaaa
     1261 caagccttac aagatgatta tattttgcaa gcctatacta ataaaggcga actgcaaaat
     1321 atcattacca tattacagag caatgacaat aacagcaact ggattatatt tggcaaagac
     1381 cctgaaacga ttaaagaggt tttcagctca ttttttcagc ccattattgc tgcaggtgga
     1441 gtagtgctaa atgaaaatca tcatgtattg ttaattttca gaagaggttt ttgggattta
     1501 cccaaaggaa aagtggatga aggtgaaagt atagatgaag ccgcacttcg tgaaattgaa
     1561 gaagaaacgg gattaaacca actcaatata ttacaacctg tcacatttgg cttctccaat
     1621 caggattgca cctatcattc atatgtgcta aacggagtga attgccttaa acagacatat
     1681 tggtttatga tggaaaataa tggcaatgat ttattgttgc ctcaaacaga agaagatatt
     1741 gaacaagcca tttgggtaca caaagacaat ttgcataatt attacgataa tatgtacgaa
     1801 agcatcaaag atgtcttgca agtagccatt acccattttt agtaacaggc ataattgatt
     1861 aaataatata gcgtggatct gtaaatgatg aggacattgg acttacttca tcttgcagta
     1921 aaaccagatg acacatacaa cacatactta agcggtcagt taaaatttca ttttttatct
     1981 tctatatatt ttgtacttgt tttttggtat attgtgagat aaatttaatt tctcatgatt
     2041 tacgatttta tcgtggtggg ttcaggttcc ggtggcggag cattagccta ttatttacat
     2101 caaggcggag caaaagtact actgattgaa gcaggaaaat tttacagaaa aaacaccttt
     2161 ccaaaaacag aagcagaaac atcagcttat ttatactggg gcggaggaat tgaatttgcc
     2221 aaagatggca gaatgggttt tctgcgaact aaaatggtgg gcggttcatc tatcgtgtat
     2281 caatgtctga tggacagatt tgatgatatc gcttttaatg actggaaagc tgaaagcggt
     2341 gtggactggt ttaattctgc cgaaatggat aaacattaca atcaggtaga aagtgattta
     2401 gtgttgcata agtttaccga aaacgaacga aatggaaatg ctcgattata tactaaagct
     2461 tgcgaccaac tcggttataa atggaaatat ttgcgaagag gacaaagtga ttgtggagca
     2521 gaacatggta atgactgcat cggatgcctt ggcggctgct tccgtgattc taagcaaagt
     2581 tcattagtag cttttattca aaaagcagaa aaaacaggat tggaaattag cacagaaaca
     2641 gaaatacatc aagtagcacc caatgctgat acgtgtaaaa tatccggaat acaaaacggc
     2701 aacaaggtaa gttttgaagg aaagaaagta atattggcgg gtggtgcatt tggaactaca
     2761 cgcatgatga ccatgagtgg atttaaacag aaatttcctg ttttgggtaa aaaatttgcc
     2821 acacatcccc agtttatgtg gttgggtttt caaaacgaaa tagtagatgc tcataagctg
     2881 atgttccaat cagtagcatc ggatgatcct gaatttcgca aaaatggctt taagctcgaa
     2941 aatgtatttg catcgcccat ttctatcggt atgcttatga atgtaaccgg ggcaaaacac
     3001 cagtatttca tgaaacaata ccgctacatg caatgtatag aagtagcagt acgcgatgaa
     3061 aacgtgggcg aaattcaatg tgataacaaa ggcaaactga ccatcgtaaa accgatgact
     3121 caacaagata aagaccgcaa aaacaaagga ttacaagcta taaaaaatat attgactact
     3181 gccggagcca aagaagtttt tgaatccgat ttttatttcg gattgcattt aatgggcggc
     3241 tgtgtgatgg gaacagatgc tgcaaaatct gttgtcagtc cggagtttaa tgtacatggt
     3301 tacaatacat tgtatatctg cgattcgagt atttacccaa atgcgccggg catcaatccg
     3361 ggattaacga ttaaatcttt aggtcatcgt ctttccactc aattattaaa ataatgcaca
     3421 aacgccatct ttttttgttt tgcattgcaa gggtatttcc ggtacgacaa ataattctga
     3481 agagaagtaa ccgcattgta agtattgaaa ataaaatcaa atgaatccaa tacgatacta
     3541 attaaaatat ctccaaatga agaacgctct tattttattt tttataagct ccatgctgtt
     3601 tctctcctgt aaaaaagaga atgacaacaa cggagatttt tcggtgctga cctacaatgt
     3661 tgccggctta cctcaaggat ttaatgccga ccagtttccg gttttacata tgagtgatgt
     3721 cgggaaattg ctaaataaag tcgatatcgt acaagtacag gaagactttt gctaccatga
     3781 caggttgctg gtgcaaaatt acgcacatcc gtttgttaca cccaccaaag gatgtgttcc
     3841 caacggcgat ggactcaata cgttttcaat ctatccgatt aatgacttat ttcgccaaaa
     3901 gtggaatgcc tgcaacgggt ttgattgttt gacgccaaaa ggatttagtt ttacccgtat
     3961 cgcgattaat aaaaaagtta gtattcattt ttataatgta catgccaatg caggaagtga
     4021 taacgccgac atggcagcca acgaaacaat attgttcagt tgatgcgata tattcaaacc
     4081 cactctgcaa atatgcccgt tattgtaatg ggcgatttta acagcagata tacacgtatg
     4141 ggtgatacga taggattatt ttacgataac ggatttaaag atgtgtggat agaaaaaatc
     4201 agaaacggtg taataccttc gaaaggcgca tcgctcatgg attgcacagg tgataaaagc
     4261 aacccggaat gcgaagtagt cgataaaata ttttacagaa gcagtgcgg
//
LOCUS       DAOQII010000222         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12447_length_4282_cov_4.717294, whole genome shotgun sequence.
ACCESSION   DAOQII010000222 DAOQII010000000
VERSION     DAOQII010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12447_length_4282_cov_4.717294"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..930
                     /gene="kdsA"
                     /locus_tag="PLF48_09735"
     CDS             163..930
                     /gene="kdsA"
                     /locus_tag="PLF48_09735"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNF49648.1"
                     /translation="MAEFFLIAGPCVVESEDLCFLIAERLKTICTSLKIPFIFKASYK
                     KANRSTLNSFSTIGEDKALQILANIKSKLAIEILTDVHETTDCNKVKDIVDVIQIPAF
                     LCRQTELLIAAGETGKIINIKKGQFADANTMIAAAEKVKSTGNNKVWITERGNMFGYH
                     DLIVDFRNIAQLISQKMTTILDITHSVQQPNSSTGITLGLTQYIETLAKCGIVSGVNG
                     IFLETHPEPEKALSDKHTMLALDKVEELLNILKNISV"
     gene            complement(1039..2076)
                     /locus_tag="PLF48_09740"
     CDS             complement(1039..2076)
                     /locus_tag="PLF48_09740"
                     /inference="COORDINATES: protein motif:HMM:NF007912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HNF49649.1"
                     /translation="MEYTHLSDTGVSVSKICLGTMTWGQQNTEQEAHEQLNYAIENGI
                     NFIDTAEMYAVPANPATQGLTEKYIGSWLKTQDRSKQIVATKIAGPSAATKHIRGGSK
                     FNSEHITAAINDSLTRLQTDYIDIYQLHWPERPTNYFGALGYTHHADDAVTDFKYVLE
                     ILTGFIEQGKIRYIGISNETPWGMMQFLTTAQQYDLPKIVSIQNPYNLVNRTFEIGLA
                     EMSIREKVGLLAYSPLAGGLLSAKYLQGKRPENCRYTLWPNYFGRYKHPNTMRAVEAY
                     AKLADDAGMTLPQMALAFVNSRKFLSANIIGATNLQQLKENISSINVKLSEDILKKIE
                     EIHLEFPNPAP"
     gene            complement(2080..2994)
                     /locus_tag="PLF48_09745"
     CDS             complement(2080..2994)
                     /locus_tag="PLF48_09745"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNF49650.1"
                     /translation="MNTCALTILGCNSALAEHGRNPTAQVLQMCNHTFLIDCGEGTQM
                     RIQQYNVKWFNIDHIFISHLHGDHYYGLIGLLTTFNLLKRQADLTIYAPKELKAIIDI
                     QLAASNTKLNYNLHYIETHSETPQLIFEDDTLTVHTIPLQHKIPTTGFLFREKIGNRK
                     LLIEKLEGRSYQKADLKLLKRNTDILDDDGNPIFAREVTADPNPAKSYAFCSDTMYNE
                     TMFDQIRGVDLLYHESTFLHDSLQRAEETRHSTALQAGLTAKEAQVKQLLIGHYSSRY
                     ANLTPLLEEAQTAFGNTILAVEGQTYQF"
     gene            complement(3021..3377)
                     /locus_tag="PLF48_09750"
     CDS             complement(3021..3377)
                     /locus_tag="PLF48_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF49651.1"
                     /translation="MKFSVDKQPDYVAYELLDDKLTSLNAPVLKSELVVANAEGFKNM
                     IVDLGNVKFVDSSGLSSLLIGHRLCKDEKGKFCLCGLNDTVKSLIKISKLDDVLHIYS
                     TQHDAIDALSKKKKAH"
     gene            complement(3517..>4282)
                     /locus_tag="PLF48_09755"
     CDS             complement(3517..>4282)
                     /locus_tag="PLF48_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HNF49652.1"
                     /translation="DKTYVSEVCTSIDKLDKVAWTEVEKELSEKNIPTASIQKLHDLI
                     SKEITLEAIKTELSGIEIAQEGIRELEEIFNYLQVLQVPATSYTFDISLARGLDYYTG
                     PIYEIKLPNHPHIGSLGGGGRYNGLIAYFGGRDLPSVGTSLGLDRIYTALEQLNLLTT
                     EKTSTKVLVCNFGKSTLQASLEWTAELRKNGIASELYPDATKMGKQFTYCDKKQIPFV
                     CIIGEEEVQSGKIGFKNMHTGTQEKLTKEEILALLK"
BASE COUNT         1321 a          788 c          747 g         1426 t
ORIGIN      
        1 agaaatcaac tatttttttc accttaattt tcttaacccg aactcaggtt tataactaag
       61 aaatcaaatt acgaaaacta ctcatttgaa cattttaatg tttcaaatta tttctgaaat
      121 ttatccttat tttagcaaag aatgataaat taaaacttcg atatggcaga atttttttta
      181 attgctggtc cttgtgttgt agaaagcgaa gacttgtgtt ttttaattgc tgaaagacta
      241 aaaacaatat gcacatcctt gaaaattcca tttattttca aagcatctta taaaaaagcg
      301 aatcgcagta cattaaactc attttctaca ataggggagg ataaagcttt gcaaatactc
      361 gcaaatataa aatcaaaact ggctattgaa attttgactg atgtacatga aacgacagac
      421 tgcaacaaag taaaagacat cgtggatgta attcagattc ctgcattttt gtgcaggcaa
      481 actgaactct taatcgctgc cggagaaaca ggaaaaatta tcaatatcaa aaaaggacaa
      541 tttgctgatg ctaatacgat gattgccgct gccgaaaaag taaaatctac cggcaataat
      601 aaagtctgga ttactgaacg tggcaatatg tttggctatc acgatttaat tgtcgatttc
      661 agaaatattg ctcaactaat atcacagaag atgactacca ttttagatat cacgcattcg
      721 gtgcaacagc ctaattcttc gacaggaata actttaggtc tgacacaata cattgaaacg
      781 cttgccaaat gtggtatagt ttcaggggta aatggtattt tcttagaaac tcatcctgaa
      841 ccggaaaaag cacttagcga taaacatacc atgctcgctt tagacaaagt ggaagagttg
      901 ttgaacatat taaaaaacat atctgtctaa atggtaagaa ttatatttgc gataagtttc
      961 tttatgatta gctgaattca aataataaat actacaaacg gagttttatt caaaattcaa
     1021 aatctataat ttcaaatttt atggcgcggg gttcggaaac tccaaatgga tttcttctat
     1081 tttctttaaa atatcttctg atagttttac gttgatgctg gaaatatttt ctttcaactg
     1141 ctgtaaattg gttgctccaa tgatatttgc acttaaaaat tttctggaat ttacaaaagc
     1201 caaagccatt tgcggcaatg tcattccggc atcatctgcc agcttcgcat acgcctctac
     1261 tgcacgcatg gtattcggat gtttatatct tccaaaataa tttggccata aggtataacg
     1321 acaattttcc ggacgcttgc cctgcaaata tttagcactc aacaagcctc cggctaaagg
     1381 cgaataagcc agcaagccca ctttttcacg tatcgacatt tcagctaatc caatttcaaa
     1441 agtgcggttc actaaattat acggattttg tatagaaact attttaggta aatcatattg
     1501 ttgcgcagta gttaaaaatt gcatcattcc ccagggcgtt tcattggaaa taccgatata
     1561 acgaattttc ccttgctcaa taaaaccggt caaaatttca agcacatatt tgaaatcggt
     1621 aactgcatca tcagcatgat gtgtataacc caaagcccca aaataattgg ttggtctttc
     1681 cggccagtgt agctgataga tgtcgatata atcggtttgt aatctggtta agctgtcgtt
     1741 tattgcggca gtaatatgtt cgctgttaaa cttagaacca cctcgtatat gcttagttgc
     1801 tgcacttggt cctgcaattt ttgtagcaac aatctgtttg cttctgtctt gagtttttag
     1861 ccaagaacca atatattttt cagtcaaacc ctgcgtagca ggattggcag gaacggcata
     1921 catctcggca gtatcaataa aatttatccc attttctatg gcataattaa gttgttcatg
     1981 tgcctcctgt tcggtgtttt gctgtcccca tgtcattgtt cccaaacaaa ttttactgac
     2041 actcacaccg gtatctgaaa gatgagtata ttccataaat taaaattgat aagtttgtcc
     2101 ttcaacagct aaaattgtgt ttccaaatgc tgtttgtgct tcttccagca aaggtgttaa
     2161 attagcatat ctggacgaat aatgaccaat taagagttgt tttacttgtg cttcttttgc
     2221 tgtcaatcct gcctgcaagg ccgtactgtg tctggtttct tcggcacgct gcaaggaatc
     2281 atgcagaaaa gtagattcat gatataataa gtctacacca cgaatttggt caaacatagt
     2341 ttcgttatac atcgtatcgc tgcaaaatgc ataactctta gccggattag gatcagcagt
     2401 tacttcacgg gcaaaaatag gattgccgtc atcatccaaa atatccgtat ttcgtttcaa
     2461 tagttttaag tcagcttttt gataactgcg tccttctaat ttctctatca gcaactttct
     2521 gttaccaatt ttttctctga ataaaaatcc ggtcgtcgga attttatgtt gtagagggat
     2581 ggtgtgaaca gtcagggtat catcttcaaa aatcagctga ggcgtttctg aatgtgtttc
     2641 gatataatgt aagttgtaat ttaattttgt gttgctggct gctaattgaa tatcaataat
     2701 agctttcaat tctttgggtg catatattgt caaatctgct tgccgcttta ataaattaaa
     2761 agtagtcagt aaaccaatta atccgtagta atggtcgccg tgtaaatgag aaataaaaat
     2821 atggtctata ttaaaccatt ttacattgta ttgctgaatg cgcatttgtg ttccttctcc
     2881 acaatctatc agaaacgtat ggttgcacat ttgcaaaacc tgtgccgtag ggtttctacc
     2941 atgttctgcc aaagcggagt tgcaaccgag tatggtcaac gcacaggtat tcataataag
     3001 cagtggtatt caatgatcaa ttagtgtgct tttttctttt tgctcaatgc atcaatagca
     3061 tcatgctgag tagaatagat atgtagcaca tcgtctaatt tggaaatttt aatcaggctt
     3121 tttacggtgt catttaagcc acaaagacag aattttcctt tttcatcttt acacaaacga
     3181 tgaccgatta ataatgaact caatcctgat gaatctacga attttacatt gcccagatcg
     3241 acaatcatat ttttaaaacc ttcggcattt gccaccacca attctgattt taagacaggt
     3301 gcatttaagg aagtcagttt atcatccaga agctcatagg ctacataatc aggctgtttg
     3361 tcgacactaa atttcatatc caatttttta caaaaataag ataatttgct taaaagattg
     3421 ccttttgata ataaaatgtc agcatacatt tatgaggtta atgatatata ttgttatatt
     3481 gaattttatc tacgtaaatt caaattgaac tgtaaattat tttaataagg cgagtatctc
     3541 ttcttttgtc aatttctcct gagtacccgt atgcatgttt ttaaaaccaa tttttccgct
     3601 ctgtacttct tcttctccga taatacagac gaaagggatt tgttttttat cgcaataggt
     3661 gaattgcttg cccatcttgg tagcatcggg gtaaagctcg gaagcaattc cgttttttct
     3721 caactcggca gtccattcca gcgaagcttg caaagtggat ttgccaaaat tgcatactaa
     3781 aacttttgta gacgtttttt ctgtagtcaa taaattcagt tgttcaagag cagtataaat
     3841 gcggtctaaa cccaaggaag tgcctacaga gggtaaatct ctgccaccga aataagcaat
     3901 cagcccatta tatctgccac caccgcccaa ggagccgata tgcggatgat taggtagttt
     3961 tatctcataa atgggacctg tgtaataatc aagtccgcgg gctaatgaaa tatcaaaagt
     4021 atagcttgta gcaggcactt gtaaaacctg caaataatta aaaatttctt cgagctctcg
     4081 gatgccttct tgtgcaattt caatgccgga gagttcggtt tttatagcct ctaaagtaat
     4141 ttctttggag attaaatcat gcaatttttg aatggatgca gttggtatat ttttttctga
     4201 aagttctttt tctacttctg tccaagccac tttatccagt ttatcaatag aagtacatac
     4261 ctcagaaaca taggttttgt ct
//
LOCUS       DAOQII010000223         4258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12558_length_4258_cov_6.216274, whole genome shotgun sequence.
ACCESSION   DAOQII010000223 DAOQII010000000
VERSION     DAOQII010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12558_length_4258_cov_6.216274"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..898)
                     /locus_tag="PLF48_09760"
     CDS             complement(89..898)
                     /locus_tag="PLF48_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNF49653.1"
                     /translation="MEKKRLLFVTHEMDPYLVLTDAAHIVKKLAPFVNDNGLEVRVLM
                     PRFGVINERRNRLHEVVRLSGINIIIDDDDYPLILKVASLPGARIQVYFLDNEEFFKR
                     KFVFHNEEEKFYEDNTERMIFFNKATMETIKKFGWAPHLIHAHGWMSSLIPLYLKTQY
                     AKDPVFANTKLIYSVYEKEINDKLGKNFIKKAMIGDNIKEKDMDTYKALTNEAFTLGA
                     VEYADAVIQGSENIKDIAAIKKAAGKKPFLGYVGLDDVTTAYLDFYKQLLQ"
     gene            998..1849
                     /gene="panC"
                     /locus_tag="PLF48_09765"
     CDS             998..1849
                     /gene="panC"
                     /locus_tag="PLF48_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNF49654.1"
                     /translation="MKVFQTVAELQSYLLEIKNLANSSVGFVPTMGALHAGHISLIEK
                     SNAQTDITVCSIFVNPTQFNDKKDFERYPIQVDRDIEMLIQANCDVVFIPGVEEIYPA
                     GAENKVLLDLGFVGQTLEAQHRPGHFEGVVQVVKRLLEIVQPDFLFLGQKDYQQCMVI
                     TKLVEAFHFPVKVIICDTLREQDGLAMSSRNVRLSREARQAAVKLSKSLYLIKNELKQ
                     ADLNSLLHEETEKLNADPLISVEYLKIINGKTLEVVDEYTAELPLAVLIAAKVGEVRL
                     IDNMILQ"
     gene            1937..2299
                     /locus_tag="PLF48_09770"
     CDS             1937..2299
                     /locus_tag="PLF48_09770"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNF49655.1"
                     /translation="MFVHVFRAKLHRVVVTEANLNYMGSITVDEDLLDASGILPHEKV
                     QIVNINNGERFETYVIKGERGSGQICLNGPAARKVHLGDKLIVIAYAMLSAEEAKEHS
                     PKAVYVNESNAIVNVNNL"
     gene            2317..3339
                     /locus_tag="PLF48_09775"
     CDS             2317..3339
                     /locus_tag="PLF48_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNF49656.1"
                     /translation="MKSVKSVLKILIPLALGIGLIIWFWNAMPENDKTETINAFKRAN
                     YFWVLLAPLVGFISNFIRTQRWRLMLRPLGYYPGYWNTFFSVMIMYFFNLFVPRLGEV
                     TRCSILAKYEKVPVEKSIGTMVTERLIDVICLGIVVLITLLLMGKANFLAMYDTFKAK
                     TAGMGGSTIMTVLKWSIPFIIIFGLLAYSLLFIRKHGVDALKEQMKNKIKSLMISVFS
                     VKDIKEMPQFLLLTVGLWVTYLVMFYMNYKALPETSNLPVVSALVCLIFGSMAVVLTP
                     GGIGIFPIIIQTILMSFGVNPNSALAIGMIAWAVQTLGMLIGGMLSLTLLSVLNKDAN
                     HETTAR"
     gene            3320..3787
                     /gene="rfaE2"
                     /locus_tag="PLF48_09780"
     CDS             3320..3787
                     /gene="rfaE2"
                     /locus_tag="PLF48_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNF49657.1"
                     /translation="MKQQHAKIFSDLSLLQENIPDNKTIVFTNGCFDLLHEGHLYLLS
                     EAGKLGDVLIVAVNSDASVKRLKGDNRPIEELAIRCKHLAELDDVQYIISFEEDTPIQ
                     LIRHLHPHVLVKGGDYAADKIAGAEFVIQCGGEVVIIPLLEGYSTTKIIAEKK"
BASE COUNT         1279 a          743 c          837 g         1399 t
ORIGIN      
        1 tcttacatgc gccgatataa agggaaatca tggcaactaa aaccggaagt aatttcttca
       61 tgttgtataa aactgctgaa tgggatgatt attgtaatag ttgtttgtaa aaatctaaat
      121 aggctgtagt tacgtcgtct aatccaacat aaccgagaaa tggcttcttg ccggctgctt
      181 ttttaatagc ggcaatatcc ttaatatttt cactgccctg aatgacagca tcggcatatt
      241 caactgcacc taaagtgaat gcttcattag tcaatgcctt gtaggtatcc atgtcttttt
      301 ctttgatatt atcgccgatc atggcctttt tgataaagtt tttacccagc ttgtcattta
      361 tctctttttc atacacacta tatatcaact tcgtattggc aaacacggga tctttggcat
      421 attgagtttt tagataaagc gggattaaac tgctcatcca gccatgtgca tgaataagat
      481 gaggtgccca tccaaatttt ttgatggttt ccatcgtggc tttgttaaag aaaatcatac
      541 gctcggtatt atcttcataa aatttttctt cttcgttatg aaaaacgaat tttcttttga
      601 aaaactcttc attatccaga aaatacacct gaatacgggc accgggcaaa gaagccactt
      661 ttaatattaa aggataatca tcatcatcaa taatgatatt tataccggaa aggcgcacca
      721 cttcgtgaag tctgtttctt ctttcgttga taacaccaaa tctgggcatc aacactctga
      781 cttccagtcc gttatcattt acaaatgggg ctaatttctt aactatatgt gccgcatcgg
      841 tcaataccag atacggatcc atttcgtgag taacaaatag taatcgtttc ttttccatac
      901 gccgctgagt caataattta taaaaatttc aaaaaatatt gtgcaaagat acggattaat
      961 agtggtaaaa ttgtttaaga aatgtaaaat atactatatg aaggtttttc agacagttgc
     1021 cgaattacaa tcttatttac tggaaatcaa aaatttagca aattcttctg taggtttcgt
     1081 gcctaccatg ggagcattgc atgcaggcca tatctcgctg atagaaaaat cgaacgcgca
     1141 gacagacatt acggtttgca gcatttttgt caacccaaca caattcaacg ataaaaaaga
     1201 ttttgaaaga tacccgattc aggtagatcg agatattgaa atgctgattc aggcgaactg
     1261 cgatgttgtg ttcattcccg gagtggaaga gatatatccg gcaggtgcag aaaataaagt
     1321 gctactggat ttgggatttg tggggcaaac acttgaagca cagcaccgtc ccgggcattt
     1381 tgaaggcgtc gtacaagtcg ttaaacgctt attggaaatc gtgcaacccg attttttgtt
     1441 tttggggcaa aaagattacc agcaatgcat ggtcattact aaactggtgg aggcttttca
     1501 ttttccggta aaggttatta tttgcgatac cctgcgagag caggatggtt tggcgatgag
     1561 cagcaggaat gtgcgtcttt cacgtgaagc caggcaagca gcggttaaac tttccaagtc
     1621 actttatctc atcaaaaatg aacttaaaca agccgactta aatagtttgt tgcatgaaga
     1681 aactgaaaag ctgaatgctg acccgcttat atcagtagaa tatctgaaaa tcatcaatgg
     1741 aaaaacactg gaagttgtgg atgaatatac agcagaactg cctctcgctg tactgattgc
     1801 cgccaaagta ggcgaagtaa gactaattga caatatgatt ttgcagtaag cccaatttcc
     1861 ggcaattaca atttaactat ccggtaattg caatgccgat tattaaccct atttttacac
     1921 cgaaaattta tgacttatgt ttgttcatgt ttttagagca aaactgcatc gtgttgtagt
     1981 aacagaagcc aaccttaatt atatgggaag cattaccgtg gacgaagatt tactggatgc
     2041 ttccggcata ttgccacacg agaaggtgca gattgtcaat attaataacg gcgagcgatt
     2101 tgaaacttat gttatcaaag gcgagcgcgg ttcggggcaa atttgtttga atgggcctgc
     2161 tgcacgtaag gttcatcttg gcgacaagct tattgttatt gcttatgcca tgctcagcgc
     2221 agaagaagcc aaagaacatt ctccaaaagc cgtttatgtt aatgaaagca atgcaattgt
     2281 gaatgtaaat aacctttaac ggttctatca acgcttatga aatctgtaaa atccgtactt
     2341 aaaattttaa ttccattagc cttaggtatc gggttgatta tatggttctg gaatgccatg
     2401 cctgaaaatg ataaaacaga aacgataaac gcctttaaac gtgccaatta tttttgggta
     2461 ttgcttgctc ctcttgtcgg ctttatcagc aattttatcc gtacacagcg atggagacta
     2521 atgctccgtc cacttggcta ttatccgggt tattggaata ccttcttttc tgtgatgatt
     2581 atgtattttt tcaatctctt tgttccgagg ttaggtgaag ttacccgttg ttcaattctg
     2641 gcgaaatatg aaaaagttcc ggtagaaaaa tctatcggta ctatggtaac agagcgcctg
     2701 attgatgtaa tctgtctagg aattgttgta ttaattactc ttttgctcat gggtaaagcc
     2761 aactttttgg caatgtatga tacttttaaa gctaagacag ccgggatggg tggcagcaca
     2821 atcatgacag tattaaaatg gtcaattcca tttattatca tattcgggtt acttgcatat
     2881 tcattattat ttattcgcaa acatggtgtt gatgccttga aagaacaaat gaaaaacaag
     2941 attaagagcc taatgatttc tgttttttcg gtgaaagaca tcaaagaaat gccgcagttt
     3001 cttttgctta cagttggatt gtgggttacc tatttggtta tgttttacat gaattataag
     3061 gcactacccg aaacgtcaaa tttacctgtt gtatcagctt tggtatgtct tattttcgga
     3121 tcgatggcag tcgtactcac accgggaggt ataggcattt ttcctatcat tattcaaact
     3181 attttgatgt cttttggcgt gaatccaaac agtgcattag caatcggtat gattgcctgg
     3241 gcggtgcaaa cactcgggat gctgataggg ggtatgctct ccttaacttt attgagtgtc
     3301 ttaaacaaag atgctaacca tgaaacaaca gcacgctaaa atctttagtg atttatctct
     3361 tttacaagaa aatattcctg ataataagac tattgttttc acgaatggtt gttttgattt
     3421 gttgcacgaa ggacatttgt atttattaag cgaagccgga aaattaggtg atgttttaat
     3481 tgtggccgtt aattcggatg catctgtaaa aagactaaaa ggagataatc gtccaattga
     3541 agaactagcc attcggtgca agcatctcgc tgaattggat gatgtccagt atattatttc
     3601 atttgaagaa gatacaccca ttcaacttat cagacacttg catccccatg ttttggtaaa
     3661 agggggtgat tatgcagctg ataaaattgc aggagcagaa tttgtgattc aatgcggcgg
     3721 agaggtcgtt atcattccgt tgctggaagg ttattcaacg acaaagataa ttgcagaaaa
     3781 aaagtaacgc ttatcgttta aagaccatct actttattct ccagagttcg gtaaaagtat
     3841 ttcaatttta aaatagttga ttttttatat agaaccacct cctattaatt cctcctcctt
     3901 gttaagttgg aggataaaaa aggttaaatc aattatttta cagttgacta aacttaacga
     3961 ctttgtattt aaatatacaa tttttatctt gtttttgggt tagctttttt ggttattcat
     4021 gttgccgtgt ttttgtattc ttcccctttt gtcttgatac aaaagtggag caataaatca
     4081 agaacaataa aaggcctttt ttagtaatta taatttttct gtatttgtgt actagtttag
     4141 gaattcagtt taatgtaaat tgtttgtttt tgtcatagaa atcaactgcg aacgtagtga
     4201 gctagatttt caaatttgac gttaaaattc gcacttttca taatttgctt tatagcta
//
LOCUS       DAOQII010000224         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12605_length_4246_cov_6.183488, whole genome shotgun sequence.
ACCESSION   DAOQII010000224 DAOQII010000000
VERSION     DAOQII010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12605_length_4246_cov_6.183488"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1219
                     /locus_tag="PLF48_09785"
     CDS             257..1219
                     /locus_tag="PLF48_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNF49658.1"
                     /translation="MSLQQITFTDKKDTRSGFGVGIYEAGLANPNVVALCADLIGSLK
                     LEKFIEKFPDRFIQTGIAEANMIGIAAGLTIGGKIPVATTFANFGTGRVYDQIRQSVA
                     YSDKNVKICCSHAGLTLGEDGATHQILEDIGLMKMLPNMTVVVPCDYNQTRLATKTII
                     EREGPTYLRFGRPVWPIFTPAEDPDFQIGKAQYLSEGKDVTIIACGHLVWHAVLAAQE
                     LERRGVSVELINMHTIKPLDEAAILQSVKKTGCVVTAEEHQQAGGLGESVASVLSRKH
                     LAPIEMVAVQDTFGESGTPDQLLEKYGLMPKDIIEKVEKVLERK"
     gene            complement(1268..2116)
                     /locus_tag="PLF48_09790"
     CDS             complement(1268..2116)
                     /locus_tag="PLF48_09790"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF49659.1"
                     /translation="MSNQPIVILGMHRSGTTMVTKLLENLGLFVGAEKEPNNEAMFFW
                     EINNWIFDLHTATAEKPYNMQFTNPACRKVIEESLHYHVQSRRKKKFLGALNNQFSSI
                     TELDIPWGWKDPKNTFTLDFWKSVFPNPKIIHIYRNPVDSIASYIERDLVLKNKFEWN
                     WKKKLKRNLLVSKNFHENFRIYDFEQGYLVWKAYVEKASSLKYDNTDFIEIKYEDFLS
                     EPEIYLAQLLDFCGLSPDENARYIQIKQLKKDRAYAFTQNEKLYAFYQQIKEDNIVKY
                     LGYGDL"
     gene            2157..2846
                     /locus_tag="PLF48_09795"
     CDS             2157..2846
                     /locus_tag="PLF48_09795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNF49660.1"
                     /translation="MSIDILYEDNHLLALNKNSGDIVQADITGDAPVSDEIKAYLKSK
                     YTKPGNVFLGVIHRLDRPVSGAVLFARTSKGLSKMNELFKTKAVKKTYWAVVKNKPPK
                     NEDTLVHYHIKDEKAKRARLFDKEVAHSKICKLHYSILCSSDNYHLLEIQLETGRFHQ
                     IRAQLAKIGSPIKGDIKYGFDRPNEDKRSIHLHARRLDFLHPVKQEPVSITAPVPKEK
                     LWQYFEEKLKT"
     gene            complement(2854..2988)
                     /locus_tag="PLF48_09800"
     CDS             complement(2854..2988)
                     /locus_tag="PLF48_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49661.1"
                     /translation="MRNVLENLLHGQGELFQKEQGVISLFPAFISCFIAIQLVYSPAD
                     "
     gene            3343..>4246
                     /locus_tag="PLF48_09805"
     CDS             3343..>4246
                     /locus_tag="PLF48_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNF49662.1"
                     /translation="MQNPTPYIPKNKIRIVTAASLFDGHDAAINIMRRIIQASGAEVI
                     HLGHNRSVAEIVDCAIEEDANAIALTSYQGGHMEFFKYMYDLLREKNSGQIKIFGGGG
                     GTILPSEIEQLQAYGINRIYSPDDGRAMGLQGMINDLLQQCDFPTGKDTAFDSKLLEQ
                     KNPKQIAQLISCAENYPDEFNKLFSDHSSPATDKSGGLSTDDRIPILGITGTGGAGKS
                     SLTDEIVRRFILDFPDKYVAIISVDPSRRKTGGALLGDRIRMNSIQSEHIFMRSMATR
                     QMNASISKDVTDAIQIFKAANYDLI"
BASE COUNT         1344 a          838 c          807 g         1257 t
ORIGIN      
        1 gtttgttttg tttttttgat gttacaaaag tatatggctt atacgttgtg ctacttatag
       61 aatttcacct atttcttaca tgatttatag ttgttgattt taataatcag ctacaagaaa
      121 gtatacagct tatagaataa tcatcaaaca gcataattat tttatattgt ctgcatttag
      181 cccttctttt attttgaaaa atgctgatat agccttattt ttcaaattga aattttagcc
      241 acatcaacat taaaaaatga gcttacaaca aataactttt actgataaaa aagacacgcg
      301 ctccgggttt ggagtaggta tatacgaagc agggttggcc aatcctaatg tggttgcctt
      361 atgtgccgat cttatcggtt cgcttaagct tgaaaagttt attgaaaagt ttccggaccg
      421 ttttatccaa accggtattg ccgaagccaa catgataggc atagcggcag gactgaccat
      481 cggcggaaaa atcccggtgg caaccacttt tgccaacttc ggaacaggac gtgtttacga
      541 tcaaattcgt caaagtgtag cctactcgga taagaatgta aaaatttgct gttctcatgc
      601 cggacttact ttaggtgaag acggagcaac acatcaaatt ctggaagaca tcgggctgat
      661 gaaaatgtta cccaatatga ccgtcgttgt accctgtgat tataatcaaa cgcgattggc
      721 taccaaaacg atcattgaac gcgagggacc cacttatctg cgttttggcc gaccggtatg
      781 gcccattttt acaccggcag aagaccctga tttccaaatt ggaaaagcac aatatttatc
      841 tgaaggtaaa gacgtaacta ttatagcctg cgggcatttg gtatggcatg ccgttttagc
      901 agcacaagag ctggaacgac gaggcgtttc tgttgagctg ataaatatgc ataccatcaa
      961 acctttggat gaagcagcta tcttgcagtc ggtgaaaaaa acaggatgtg tggtgactgc
     1021 tgaagaacat cagcaagcag gagggttagg cgaaagtgtg gcctctgttt tatccagaaa
     1081 gcatctggca cctattgaaa tggttgcggt gcaagataca tttggtgaaa gtggcacgcc
     1141 tgaccaatta ctcgaaaaat atggtcttat gcctaaggat attattgaaa aagtggagaa
     1201 agtactggag agaaaataaa gtaattttct aactatacta agacaaacgc aggattttag
     1261 ccttcaacta caagtcgcca tatcccaaat attttacaat attatcttct tttatttgct
     1321 gataaaaagc atatagtttt tcattttgtg taaaggcata cgcgcggtct ttttttagtt
     1381 gcttgatttg gatgtatctc gcattttcat ccggagatag tccgcaaaaa tccaacaatt
     1441 gagccagata aatttcaggt tctgataaaa agtcttcata ttttatctct ataaaatctg
     1501 tattatcgta ttttaacgat gaagcctttt ctacataagc tttccagacc aaataacctt
     1561 gttcaaaatc ataaatcctg aaattttcat ggaagttttt actgaccagc aaattgcgct
     1621 tcaacttttt tttccagttc cactcaaact tattctttag cactaaatcc cgctcaatat
     1681 aactggcaat actgtcaacc ggattacggt aaatgtgaat gatttttgga tttgggaaaa
     1741 cggacttcca gaaatcaagt gtaaacgtgt ttttaggatc tttccagccc cacggaatgt
     1801 ccagttctgt aattgatgaa aactgattat taagtgcacc aagaaacttt tttttccttc
     1861 gggattgaac atgataatgc agactctctt caattacctt gcgacaagca ggattggtga
     1921 actgcatatt atacggtttc tcagctgtag ccgtgtgtaa atcaaaaatc cagttattaa
     1981 tttcccaaaa aaacatagcc tcattattcg gctctttctc agcacctaca aacaaaccca
     2041 agttttccaa taactttgta accatagtag taccactgcg gtgcatgcct aaaatgacta
     2101 ttggctgatt cgacatgttg taaaattagc ctaactttgt taaactaaag aattttatgt
     2161 caatagatat cctttatgaa gataatcact tgttagccct aaacaaaaat agtggagata
     2221 ttgtgcaagc tgatattacc ggcgatgctc ctgtaagtga tgaaattaag gcctacctga
     2281 aatccaaata cacaaaaccg ggcaatgtat tcctgggggt tatacatcgc ctcgaccgtc
     2341 ctgtgtcggg tgctgtattg tttgcccgca ccagcaaggg cttgagcaag atgaatgagt
     2401 tgtttaagac taaagcagtt aagaaaacct attgggcggt agtaaaaaat aaaccgccaa
     2461 aaaatgaaga cacattagta cattatcaca taaaagacga aaaagcaaaa cgtgcaaggt
     2521 tgtttgataa agaagtggct catagtaaaa tttgtaaact gcattattca atactttgta
     2581 gttcggataa ttatcatttg cttgaaattc aacttgaaac aggtcgattt catcagatta
     2641 gagcccagtt agcaaaaatt ggcagcccaa taaagggtga tatcaaatac ggttttgacc
     2701 gcccgaatga agataagcga agcatacatt tacatgcccg tcggctggat tttttacatc
     2761 cggtaaaaca agaaccggta agcataaccg cacccgtgcc gaaagaaaaa ctatggcagt
     2821 attttgaaga aaagttgaag acataaatac aattcaatcg gctggtgagt acaccagctg
     2881 aatagcaata aaacaactaa taaaggcagg aaaaagtgaa attacacctt gttctttttg
     2941 aaaaagttca ccttgcccat gtaggagatt ttctaataca tttctcattc gtcatcattt
     3001 tataaaagtc tattcctcta tttgaattca aaaaactaca attggtttta tattcatctt
     3061 gctaaaataa aatattttct atctgatatt tgctattcta caattgtatc acttatttaa
     3121 gaacacggaa tacacaaacg gtactgattt ttatctgtat ttatctgtcc tatccgtttt
     3181 ttctgtgttc aatattaatt tatgattcat aacgactacc agaacgtagt tttcttatct
     3241 attccttata gaaaatgtta tttcaataac gcccactcgc tatgcattat gttattttaa
     3301 actctaaggc ggatttagta aatttgttca atagttcagc ttatgcaaaa tccgacaccc
     3361 tatattccaa aaaacaaaat ccgcatcgtt accgcagcct ctctgttcga cggacacgat
     3421 gccgccatca atatcatgcg acgcatcata caagccagcg gggcagaggt catacatctc
     3481 ggtcataacc gctctgtagc cgaaatcgta gattgtgcca ttgaagaaga tgccaacgcc
     3541 attgccctca cctcttatca gggcggccac atggagtttt ttaaatacat gtatgatttg
     3601 ctgcgcgaaa aaaacagcgg acaaattaaa atcttcggcg gcggcggcgg tactatcctg
     3661 ccttccgaaa ttgaacaatt acaggcttat ggcatcaacc gcatttactc acccgatgat
     3721 ggcagagcga tgggtttgca aggcatgata aacgatttgt tgcaacaatg tgatttcccg
     3781 acaggaaaag atacagcttt tgacagcaaa ttactcgaac aaaaaaatcc taaacaaata
     3841 gcccaactga tttcctgtgc tgaaaattac cctgacgagt ttaataaact gttttcagac
     3901 cacagctcac cagctacaga taaaagtggt ggactgtcaa ctgacgaccg aataccaata
     3961 ctgggtatca ccggcacggg cggagccggc aaatcctcgc tgaccgatga aattgtacgt
     4021 cgttttatcc tggattttcc ggataaatat gtggctatta tttctgttga cccatcacgc
     4081 agaaaaacag gaggggcact actgggcgac cgtattcgca tgaacagcat tcaaagcgaa
     4141 cacattttta tgcgctccat ggctacccgg caaatgaatg cttcgatatc taaagatgtt
     4201 acggatgcta ttcagatttt taaagcggca aactacgatt tgatta
//
LOCUS       DAOQII010000225         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12644_length_4238_cov_5.132680, whole genome shotgun sequence.
ACCESSION   DAOQII010000225 DAOQII010000000
VERSION     DAOQII010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12644_length_4238_cov_5.132680"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1447
                     /locus_tag="PLF48_09810"
     CDS             197..1447
                     /locus_tag="PLF48_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49663.1"
                     /translation="MSYFSNLAADFVQHWYVYISMPFIAALIGYITKIVAIEMMFRPI
                     EFFGIKPFLGWQGIIPRKAAIMSGIACDTLTSRLIKPEEIFSRLDPDRVAKELEKPML
                     PIVEKITHEVMNHYQPDLWESIPENIKRMLIRRIQQESPEMVRELMQQTKDNLNEIFD
                     LKDMVITNLTKDKTLLNKIFLESGAKEFQFIRNSGIYFGFLIGLVQMIAYLLTKNAWV
                     MPIFGLFTGWFTDWLALKMIFTPRTETKYLGIFKWQGLFFKRQQEVSAAYGALIAKEI
                     LTPSNMIESILKGKLSDNLFTMIHKNIQKMVDEQAGALKPVVVFAVGSQRYREMKDVI
                     TEKMVLQLPVALKHIEKYAEDAMDIQNTLVNKMQELTPEEFEGVLRPAFKQDEWILIT
                     VGAVLGFLVGLLQDFTVEHFLHNM"
     gene            1458..2516
                     /locus_tag="PLF48_09815"
     CDS             1458..2516
                     /locus_tag="PLF48_09815"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49664.1"
                     /translation="MSELLSEGKTTWRSPSNIALIKYWGKHGRQLPRNASISFTLSQA
                     YTETSLLFEKKKETSDAISLEFLFEQKKQEAFEIKLKKYLTEMLEFIPVLSQYHLRIQ
                     SSNSFPHSTGIASSASSMSALAMCLTDMENEILHPETETSLRLQKASKLARLGSGSAC
                     RSIYPSMAVWGKHPHIPDSADEYAIPYTDHIHPVFKTYKDAILIVSSTEKEVSSRAGH
                     ALMENNYFASSRYRQADDNMSEIIIALKSADLEHFCEIVENEALTLHALMMASQPSFM
                     LLKPNTLKIIDLLKTFRKENNVPLCFTLDAGPNLHLLYPQEYALQAETFIEQELLGYC
                     ENRKVIYDSVGIGPEKIN"
     gene            2519..3466
                     /locus_tag="PLF48_09820"
     CDS             2519..3466
                     /locus_tag="PLF48_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49665.1"
                     /translation="MTSKEQTYNSKILLFGEYSIIQDSMGLTVPFSEFQGQLTFVADE
                     NNPEFSQKSNQHLAAFSNYIEKCLDNQSLAIDFDIQAFKKDIEQGIYFKSSIPQGFGV
                     GSSGALVAAIYGKYTRNKIEFNDKPTKEEVFKLKQILAKLESYFHGNSSGVDPLICYL
                     NLPLLINGKENIEPVSVHLKNNGKGAIFLIDTGISRSTEPLVKYYMQKMEEPTFREFV
                     DKQLKPFANNCIHAFLDGDKETLFAYLKLLSKFQFEHFKPMIPSLFNKLWKRGLDTGE
                     YYLKLCGAGGGGYILGFTRNFEHTKKQLKNQTLELLCHI"
     gene            3912..>4238
                     /locus_tag="PLF48_09825"
     CDS             3912..>4238
                     /locus_tag="PLF48_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49666.1"
                     /translation="MAQAKTNNKSVGLGAGMVVLISVIFTNVLFYTVFKGQVDEKGHP
                     KNIIGLFYQGGVIVPFLMAIFIICITFFIERLLTISRAAGTGSLDTFLQSIKTRLNNN
                     DIEGAIQ"
BASE COUNT         1370 a          776 c          778 g         1314 t
ORIGIN      
        1 aattttactc aataaactaa atctatctat tacctaaaaa attttagggt ttgagtttaa
       61 ttgaaacgct tatcccgaac tcaggttagt aaagggtaaa agggaaattt atttgacatg
      121 aatgtacttg ttttcatata aataatcatt ttgacaggta gcagttttta ccatactatt
      181 tttctacttt tgcaggatga gttatttttc caatctcgct gctgattttg ttcagcattg
      241 gtacgtatat atctccatgc ctttcatagc tgctttaatt ggttatatta caaaaatagt
      301 tgctatagaa atgatgtttc gaccgataga attctttggc atcaaaccat ttcttggctg
      361 gcaaggcatt attccgcgca aagcggctat catgtcgggt attgcctgcg atactttgac
      421 atcccgatta ataaaaccgg aggaaatctt ttcgaggctg gatcccgacc gcgtagccaa
      481 ggaactggag aagcccatgt tgcctatcgt ggaaaaaatc acccacgagg taatgaacca
      541 ctaccaacct gacttgtggg aatctattcc tgaaaacatc aaacgtatgc tgattcgtcg
      601 tattcaacaa gaatctcctg aaatggtgcg tgagttgatg caacaaacca aagataatct
      661 caacgagatt tttgacttga aagatatggt gattactaat cttaccaaag ataaaaccct
      721 gcttaacaag atatttttag aaagtggtgc taaagagttt cagttcatcc gcaattccgg
      781 aatttatttc ggctttctta ttggtctggt gcaaatgata gcctatctgc ttactaaaaa
      841 tgcctgggta atgcccatat ttggtttatt taccggatgg tttaccgact ggctggcttt
      901 gaaaatgatt tttaccccaa gaactgaaac aaagtatttg ggtattttta aatggcaagg
      961 tctttttttt aaacgccagc aagaagtttc cgcagcatat ggtgcattaa ttgccaaaga
     1021 aattctcact ccaagcaata tgattgaatc tatattaaaa ggtaagttat ctgacaatct
     1081 ttttactatg attcacaaaa acattcaaaa aatggtagat gagcaagcag gggctctcaa
     1141 accggttgtc gtttttgctg taggttcgca acgctatcgc gaaatgaaag atgtcattac
     1201 agaaaagatg gttttacagt tgcctgtagc tctaaaacat attgaaaaat atgctgaaga
     1261 tgccatggat attcagaata cactggtgaa caaaatgcag gaactcactc ctgaagaatt
     1321 tgaaggcgtt ctgcgaccgg ctttcaaaca agatgaatgg attttaataa cagtcggtgc
     1381 cgtattgggt tttcttgtag gcttgttgca agatttcacg gtagagcact ttttacataa
     1441 tatgtaatat ataggaaatg tccgaattgc tatctgaagg aaaaacaaca tggcgcagcc
     1501 catctaatat tgcactgata aaatactggg gaaaacacgg caggcaactt ccccgaaatg
     1561 ccagcatcag cttcacgctt tctcaggctt ataccgaaac cagtctttta ttcgagaaaa
     1621 agaaagagac atctgatgcc atctctcttg agtttttatt tgagcaaaaa aagcaagaag
     1681 cctttgaaat taaactaaaa aaatacctga cagaaatgct ggagtttatt cctgtattat
     1741 cacaatatca tctccgtata caaagttcca attctttccc gcattctacc ggtatagcca
     1801 gttctgcttc atccatgtcg gcattggcca tgtgcctgac ggatatggaa aatgaaattc
     1861 ttcatcccga aactgaaaca agtttacgat tgcagaaagc aagtaaactg gcaagattag
     1921 gaagcggttc agcatgtcgt tccatttacc catctatggc agtttggggt aagcatccgc
     1981 atatcccaga ttctgctgat gaatatgcca tcccttatac cgatcatatt catcctgttt
     2041 ttaaaactta taaagatgcc attttaatag tttccagtac ggaaaaggaa gtttccagcc
     2101 gggcaggaca tgctttgatg gaaaataatt attttgcatc cagccgatat aggcaggctg
     2161 atgacaatat gtcggagatt attattgctt taaaatcagc cgatttagag catttttgcg
     2221 aaattgtaga gaatgaggca cttaccttac atgccctcat gatggcttca cagcccagtt
     2281 ttatgttatt aaagcctaac acactgaaaa ttattgattt attaaaaaca ttccgtaagg
     2341 aaaataacgt acctttgtgc tttactttag atgcaggacc aaatctgcat ttattgtatc
     2401 ctcaggaata tgctctgcaa gcggaaactt ttattgaaca ggaattgtta ggttattgtg
     2461 aaaacaggaa agtcatttat gatagtgtgg gaattggacc tgaaaaaata aactgaaatt
     2521 gacttcgaaa gaacaaacat ataactccaa aattttactc ttcggagagt atagtatcat
     2581 tcaggattcg atgggactta ccgtaccgtt ttccgaattt caaggacagc ttacttttgt
     2641 tgcagatgaa aacaatcctg aattctcaca aaaatccaat caacatttag cagcgttttc
     2701 aaactatatc gaaaaatgtc tggataatca atctttagca attgattttg atatccaggc
     2761 cttcaaaaaa gatattgagc aaggcattta ttttaaatct tccattccgc aaggatttgg
     2821 tgtaggaagc agcggtgcgc tggtagccgc aatttatggc aaatacacta ggaacaaaat
     2881 tgaattcaac gacaaaccca cgaaagaaga agttttcaag ctcaaacaaa tactcgctaa
     2941 gttagaatct tatttccatg gcaacagcag cggagtagac ccgcttattt gttatctcaa
     3001 tttaccttta ctcattaatg gtaaagaaaa tattgagccc gtttccgtac atctgaaaaa
     3061 caatggcaaa ggggctatct ttctgataga taccggcatt tcgaggagca cagaacctct
     3121 ggtaaaatat tatatgcaga aaatggaaga acccactttc cgcgaattcg tagacaaaca
     3181 actcaagcca tttgccaaca actgtatcca tgccttttta gatggagata aagaaaccct
     3241 ctttgcctat ctgaaactgt tatctaaatt ccagtttgag catttcaaac ctatgattcc
     3301 atctttgttt aataaacttt ggaagcgcgg attagatacc ggagagtatt acctgaaatt
     3361 atgtggtgcc ggcggtggtg gctatatatt gggttttacc cgcaattttg aacacactaa
     3421 aaaacaactc aagaaccaaa ccttagagtt gttatgtcat atttgacagc aatactaatc
     3481 ctttttttat tttccattac agactttatt ttcagcattt ttcaatagat taagcttacc
     3541 caatttatca acctgagttc gggttaagaa aaaaagctga taaagatagt tgatttctca
     3601 atttttcctc cactttaaca aggtggagga attaaaagga ggtggttcta cataaaaaat
     3661 caactatttt tcaattgata cgcttaaccc gaactcaggt tacccaattt atcaggtata
     3721 ttctagttta attcaggtta aattttaatt ttgaggctat cagaaaaaat attcacattt
     3781 ttttgcaact tagttgcaat tttttattta tctttacgtt ggttaaaagg atgcaggatt
     3841 aaaaaaaagt tctattttta cagccaacgt aaaattaaag taaacatcat tttaaactca
     3901 aatttttaat tatggctcag gcaaaaacaa acaacaaatc tgtcggctta ggcgcaggga
     3961 tggtagtatt gatttcggta atctttacca atgtactttt ctacacagta tttaaaggac
     4021 aagtagatga aaaaggtcac ccgaaaaaca ttatcggact cttttatcag ggtggggtaa
     4081 tcgtaccatt tctgatggca atcttcatta tctgtattac gtttttcatc gaaagattac
     4141 taactatcag cagagcggct ggtacaggtt ctttagatac atttttacag tctattaaaa
     4201 ccagattaaa caataatgac attgagggtg caattcaa
//
LOCUS       DAOQII010000226         4214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12758_length_4214_cov_4.281318, whole genome shotgun sequence.
ACCESSION   DAOQII010000226 DAOQII010000000
VERSION     DAOQII010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12758_length_4214_cov_4.281318"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLF48_09830"
     CDS             complement(<1..529)
                     /locus_tag="PLF48_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF49667.1"
                     /translation="MKQIIITIFNLTIFIFVLGQTPHNMVFIKGGTYVPLYSLDSGKA
                     VVNSFYMDKYPVTNAEFEKFVLQNPQWHKNKVKAIFADKNYLKNWKTDSTFGIANKNE
                     RNSPVIYISWFAAKKYCECQGKRLLTVKEWEFVALASETKKDASGDAKFSQKILDWYG
                     KPTPKQFAEVGKGFRN"
     gene            complement(531..2012)
                     /gene="nirK"
                     /locus_tag="PLF48_09835"
     CDS             complement(531..2012)
                     /gene="nirK"
                     /locus_tag="PLF48_09835"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNF49668.1"
                     /translation="MKSKSALRLSVGIIILMSLNLFFTSCKTNSNEQKEIWSSDPKEK
                     IVGEEKAILTDPPMVPPQITRTHATKVIVELEVIEKEMEIMDGVKYNFWTFGGTVPGK
                     FIRVREGDFVEFHLKNNASSKLPHNIDLHAVTGQGGGASSTFTAPGHETVFSFTAINK
                     GLFIYHCATAPVGMHIANGMYGLILVEPKEGLPKVDKEFYVCQGDFYTKGAYGESGTQ
                     PFDMAKALEENPDYVLFNGRVGALTGDNTLKAKAGEKVRLFVGNGGPNLLSSFHVIGE
                     VFDNVRVEGGDLLNHNVQTTAIPAGGSAITEFRCEIPGNFILVDHAIFRTFNKGALGI
                     LSVTGNDNKTVYSGQLSDNVLHSEGSALQTIPEQTQLAPVAKNKDERIAKGKTVYTSV
                     CQACHQPDAKGIPNAFPPLAASDYFKGNPNKAISAVVHGLSSPITVNGKTFNGIMPQQ
                     TLSDEEVANVITYILNNFGNEGGEVTPQQVAGVRKTSAPSAAK"
     gene            complement(2049..3344)
                     /locus_tag="PLF48_09840"
     CDS             complement(2049..3344)
                     /locus_tag="PLF48_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNF49669.1"
                     /translation="MLRTFKKSIVYFLLIGSCNAVFAQHKIELSGQFIPRLEQRHGYR
                     TLAPKDSKSSVFISERIRLNFDYQNKYVRVFTSIQDVRVWGNEPQAQNYGSFGLHEGW
                     AEIFIKDKASVKLGRQELVYDDHRLLGNLDWVQAARSHDAILIKVKHKNAQLHMGGGF
                     NQSSENLLGTVYKVKNYKALAFAWYNQKFDSSRSALSVYAIADGMQINDSVPTTKFRF
                     TIGPRAEFKYKHVNANIGAYVQTGKTISDKTILAFMANVYAEYTHPKFNIGAGYDYIS
                     GQNASKTTNTKYNSFNTLYPTNHKFYGHMDYFLDLPSDTKLGGLQDVYLRLNYRPKPK
                     GMLGLDAHYFLSGNKVANPTSAGSYLKLPLGSELDAYGTYKPYDFLEVRAGYSIMAAT
                     SSMEVIKPTGSRKAYQGWSFVMISLKPSFFKWEKEAKAE"
     gene            3496..3924
                     /locus_tag="PLF48_09845"
     CDS             3496..3924
                     /locus_tag="PLF48_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNF49670.1"
                     /translation="MIFSKSCEYALRSVIYICTKSNEGINLSVKEIAAEIESPEPFTA
                     KILQQLARQKIISSIKGPNGGFYIEKQNKPVTIYQIVEAIEGPDFFDRCVLGLKNCSE
                     RKPCPLHNDFIQHRKEIKELFKTKTISDLILNQKGKINLK"
     gene            3975..>4214
                     /locus_tag="PLF48_09850"
     CDS             3975..>4214
                     /locus_tag="PLF48_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49671.1"
                     /translation="MKRKTFKWFTAICGIGFTLWMVSCNPSKTESTSSDQTTPPAATE
                     QPAATAGAGNAEAGKAIFMDPAKCSMCHKLTDEKLI"
BASE COUNT         1252 a          785 c          758 g         1419 t
ORIGIN      
        1 cattccgaaa tccttttcct acttctgcaa attgtttggg tgttggtttg ccataccagt
       61 ctaatatttt ctggctaaat tttgcatcgc cagaagcatc tttctttgtt tccgaagcca
      121 atgccacaaa ttcccattct ttcacggtta ataatctttt accctggcat tcgcagtact
      181 ttttcgcggc aaaccaagaa atataaataa caggactatt tcgttcattt ttgtttgcta
      241 taccaaatgt agaatcagtc ttccagtttt ttaagtaatt tttatctgca aaaattgctt
      301 ttactttgtt tttatgccat tgaggatttt gtaaaacaaa tttttcaaat tcagcattcg
      361 taacaggata tttatccatg taaaatgaat taaccacggc ttttccagaa tctaaagaat
      421 agagcggcac atatgtgccg cccttgataa acaccatatt atgaggcgtt tgccccaaca
      481 caaaaatgaa aattgttaaa ttaaaaatcg ttataataat ctgtttcatg ttatttagct
      541 gctgacggag ctgaagtttt tctgacacct gctacttgct gaggggtaac ttcaccgcct
      601 tcgtttccaa aattatttaa aatataagta ataacattgg caacttcttc atcacttaat
      661 gtttgttgtg gcatgatgcc gttaaaggtt ttgccattta cagtaatagg tgatgataaa
      721 ccgtgcacaa ctgcgctgat agctttatta ggattgcctt taaaataatc agaagcagct
      781 agtggaggaa atgcatttgg aattcctttg gcatccggtt gatggcatgc ctggcataca
      841 ctagtatata cagtttttcc tttcgctatt ctttcgtctt tgtttttagc tacgggtgct
      901 aattgtgttt gttcaggtat ggtctgtaga gcacttcctt cactgtgcag tacattgtcg
      961 gaaagttgac cggaataaac tgttttgtta tcatttcctg taactgacaa tatcccgagt
     1021 gcccctttat taaatgtacg gaaaatggca tgatcaacca agatgaaatt gcccggtatc
     1081 tcacatctga attctgtaat ggcagatcca cctgcaggta ttgctgtagt ttgtacattg
     1141 tgatttaata agtctccgcc ttctacccgc acattgtcaa acacttcacc aattacgtgg
     1201 aaagatgaca atagattagg tccaccgtta cctacaaata atctgacctt ttctcccgct
     1261 ttagctttca gtgtattgtc gcctgttaat gcgccaactc ttccgttaaa taaaacataa
     1321 tcagggttct cttccagtgc ttttgccatg tcgaatggtt gggtgccgct ttcaccataa
     1381 gccccttttg tataaaaatc accttgacat acataaaact ctttgtcaac ttttggcaat
     1441 ccttctttcg gctctactaa tatcaagcca tacataccat tggcaatatg catacccaca
     1501 ggtgctgtgg cacaatggta tatgaataag cctttgttta ttgctgtaaa cgaaaataca
     1561 gtttcatgtc cgggtgctgt aaaagtagat gaagcaccac caccttgtcc ggttacggca
     1621 tgtaagtcaa tattatgagg cagtttgctg ctcgcattat tttttaagtg aaattctaca
     1681 aaatcacctt cacgtacacg gataaacttg cccggtactg tgcctccgaa agtccagaaa
     1741 ttatatttta cgccatccat aatttccatt tctttctcaa tgacttccag ctctactatg
     1801 actttagtag catgagttct tgtaatttga ggcggcacca tgggagggtc ggtcagtata
     1861 gccttttctt cccctacaat tttttctttc ggatctgaag accagatttc tttttgttca
     1921 tttgagttgg ttttacagga tgtaaaaaaa aggtttaatg acatcagaat aataattccg
     1981 acggataacc ttaatgctga ttttgatttc attttgttga gtttattgtg ataaaattgt
     2041 ttatttaatt attcagcttt tgcttctttt tcccatttga aaaaacttgg ttttaatgat
     2101 atcatcacaa atgaccaacc ctgataagct ttgcggcttc cggttggttt tatgacttcc
     2161 atggaagaag tagctgccat gatggaatat cctgctcgaa cttccaaaaa atcgtatggt
     2221 ttataagtcc catatgcatc aagctcagat ccaagtggca gttttaaata gcttcctgct
     2281 gacgtgggat tggcaacttt gtttccactt aaaaaataat gtgcatcaag ccctaacatg
     2341 ccttttggtt ttggacgata gttcaaccgc agatatacat cctgtagacc gcctaattta
     2401 gtgtctgatg gtaaatccaa gaagtaatcc atatgtccgt aaaatttgtg attagtaggg
     2461 tataacgtat taaatgagtt gtatttggtg tttgtggttt tagacgcatt ttgccctgat
     2521 atataatcat agcctgcacc aatattgaat ttaggatgcg tgtattcggc ataaacattt
     2581 gccataaagg caagtattgt tttatcggaa attgtttttc ctgtttgtac gtaggcgcca
     2641 atattggcat ttacatgttt atatttaaat tcagcacgag gaccaatcgt gaaacggaat
     2701 tttgtcgtgg gaacagaatc gtttatttgc ataccgtctg caatagcata tacggaaagt
     2761 gcagatctgc tactgtcaaa tttttgatta taccaggcaa aagctaatgc tttgtaattt
     2821 tttactttgt atacagtacc taataagttt tcactgcttt gattaaatcc gccacccata
     2881 tgcaactgcg catttttatg ttttactttt attaatatgg catcgtgtga gcgggcagcc
     2941 tgtacccagt ctaaatttcc taataaacga tggtcatcat atactaattc ttgccgacct
     3001 aatttgacgg atgctttgtc tttaataaaa atttctgccc agccttcatg taaaccaaag
     3061 cttccataat tttgagcctg tggttcattt ccccaaacac gtacatcctg aatgcttgta
     3121 aatactcgta catatttatt ctgatagtca aagttaagcc ggattctttc ggagataaaa
     3181 accgagcttt ttgaatcttt cggagcaagt gttctatacc cgtgtcgttg ctctaagcga
     3241 ggtataaact gaccggataa ttcgattttg tgttgagcga atacagcgtt gcaacttcca
     3301 attaatagga agtaaacaat tgattttttg aaagtgcgta acatgtttat tttttaaaaa
     3361 agataaaagg atatttatat cccaaaatta aggtcttcaa aaattattga aactgatagt
     3421 actcaatgta aaaaatgaca tttctcatat tctgttattt cgggagtaat tgtattttta
     3481 cagctaaatc atggtatgat attttcaaaa agttgtgaat atgcccttcg ttcggtcatt
     3541 tatatttgca caaagagcaa tgaaggcatc aatttaagcg ttaaggaaat agcggctgaa
     3601 atagaatcac cggaaccatt taccgcaaaa attttacagc agctggctcg tcaaaaaata
     3661 atttcttcaa taaaaggtcc caatggtggt ttttacatcg agaagcaaaa taaacctgta
     3721 actatatatc agatagtaga ggcaatagaa ggtcctgatt tttttgatcg ttgcgtgtta
     3781 ggtttgaaaa attgttcaga aaggaaacct tgtcccttgc ataatgattt tattcagcat
     3841 cgaaaagaaa ttaaagaatt atttaaaaca aaaacgatta gtgatttaat tttaaatcaa
     3901 aaaggaaaaa taaatttaaa ataatatcgg gaattcagaa aaaaagatat ttttatccta
     3961 aaattcaatt ttatatgaaa agaaaaacat ttaaatggtt tactgctata tgcggtatcg
     4021 gattcacctt atggatggtg tcttgtaatc cgtctaaaac agaaagtaca tcctctgatc
     4081 aaactacacc gccagctgct actgagcaac ctgcagctac agcaggggca ggaaatgcag
     4141 aagccgggaa agcaatcttc atggatccgg ccaaatgctc catgtgtcat aagttgacgg
     4201 atgagaaatt aata
//
LOCUS       DAOQII010000227         4206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12798_length_4206_cov_5.157071, whole genome shotgun sequence.
ACCESSION   DAOQII010000227 DAOQII010000000
VERSION     DAOQII010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12798_length_4206_cov_5.157071"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1681)
                     /locus_tag="PLF48_09855"
     CDS             complement(296..1681)
                     /locus_tag="PLF48_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49672.1"
                     /translation="MKTFFDSFTPEDTLLGYSPLTSKSAMFFDALNSADSSKRDEAIA
                     SLDVMKFKDKNDAANYIRFLESPNYYKYNVADREDLIIKFGKIQDKSVLPKLIKMYAQ
                     SGDTSNLQFAVLDAIALQKNSKGFKFIDSIFTNEAPIPSDEDEMSDFFTDLYDTLKLS
                     AILYPGLLKLTKYDEYKDEVNDLLVELIDSNAIKPEAYAGYKNDILREANETLKKLNS
                     KEQKNQIQKNINEEDEDDYSYGSSVSTYSEKSDLLDYATMLAPFYKEANVKSFFDKLV
                     AVNDKPTRIKTYAVLLKNKIPFADTIFTNFAKNTETRYELYDELKDNKLLSAFPKQYL
                     NQLDIATSDLKSDDYKEDKDSVIFLEKVMVHTKSDSGWVYFFKCFIAKDKKYYLDYIG
                     LMPADTNKVELLSLKSKNNYSLFRSYDAAEKWKESKLPEMKDKIKDKFRLSGRLRVKD
                     PEETNSSIFDY"
     gene            complement(1678..4014)
                     /locus_tag="PLF48_09860"
     CDS             complement(1678..4014)
                     /locus_tag="PLF48_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNF49673.1"
                     /translation="MINKKLILVVTYLLLLLPTTLNAQKKYQSRFWEITGNGLSKPSY
                     LYGTMHVSKKLAYYLPDTFFVAMQRADVVALESDLNIYLDEMIKSKMFQLSTALMNQS
                     GTENLYKDAFSMKVPERNDYAELISDDPDIINEMMYRFSDLYKGQSDFEESTYLDLFI
                     FQCARRLNKKTIGLETFDGSMKYYLKAINPQNERDEEKEKKNDSIISMEDESENILDN
                     IESKLIEKVEDAYRKGDLDMLDSLDHKQYPSKLYGKYLIEDRNLVMLHSMMEVYKTGQ
                     SLFTGVGAGHLPGKNGLIELLRKEGYTVRPISLDKMKTATQTKDKLEKVKLPLTFKNF
                     TSDDKIFSVDVPGNMYEIYVFDELKQYLCIDAANAAYYTVYRIKSHNELGGYSVDVNR
                     QRIDSLFFEYIPGKILKKTEISKFGYPGFDITSKTQRGDIRRFIFIITPLEIFIFKAA
                     GAGDYFSNKENDRFFNSISLNIQPDKWSTYAPDYGDYTASLPDYRIFDKQTEHIVSNA
                     HFDKTFAYDKEGIYYSLMRANYSDYKFIEEDTFELAYPIEVMNKDWGFDVINRQFSTY
                     EKHPAFETTLKTKEGKYLFIKTIIKGPHYYFLSAVSSANKKPEQYFNSFHFVPEVYNT
                     PFFTYQDTALHYSVKTCVKPALDELVEYAGYNTQQNKYSAKEEIKPYESNIINKTYFN
                     PSTKEQIDITYEKLHDYLSIQNIDSFWNTDIDYYTKKCGLKISSYSKKEQNQITIIDY
                     VLTDTNSTRGILLKTVLRKAYLYYMASVIDTIDGQVNL"
BASE COUNT         1215 a          758 c          723 g         1510 t
ORIGIN      
        1 attttagttg atgaacatct gcttgtttgt gataggaaag tcctacaaaa tataggctca
       61 gaccatctgc ctcttgtaat taagtttgga ctaaagcatt aaaaagaatt gatatattta
      121 atgattatat ttattataaa tacggagttg attgctcaaa cgatgttcta cttatacgag
      181 taaactgagt tcgggataag cgtttcaatt ataaaatagt tgatttttta tatagaacca
      241 cctcctttta attccacccg acttgttaat gtggaggaaa aaatgcgaaa tcaggttagt
      301 aatcaaatat tgaagaattg gtttcttcgg gatctttaac cctcaaccga cctgaaagtc
      361 tgaacttgtc tttgattttg tccttcatct ccggcagctt gctttctttc catttttcgg
      421 cagcatcata agacctgaac agactgtaat tgtttttgct tttcaaactc aaaagttcca
      481 ccttgtttgt atcagccggc attagcccta tataatccaa gtaatatttt ttgtctttgg
      541 caataaagca cttgaaaaaa taaacccaac ctgaatcgga cttggtatgt accataactt
      601 tttcgaggaa aatgacagag tctttgtctt ctttgtaatc atcacttttt aggtcagacg
      661 ttgctatatc gagctggttt aagtattgtt ttggaaatgc tgaaagcagc ttattgtctt
      721 tcaattcatc atataattca tatctggttt ccgtgttttt ggcaaagttg gtgaagatag
      781 tgtctgcaaa aggaattttg ttttttaaca gcacagcata ggttttaatc cgcgttggtt
      841 tgtcgtttac agcaacgagt ttatcaaaaa atgacttcac attggcttcc ttataaaatg
      901 gagccaacat ggtggcataa tccaataaat cggatttttc tgagtaggta gatacacttg
      961 atccgtaact ataatcatcc tcatcttctt cgttaatatt tttttgaatc tgattttttt
     1021 gttctttaga attcagtttt ttcaacgttt cattggcttc tcttaagata tcatttttat
     1081 agccggcata ggcttcgggt ttaattgcat tactatcaat taattcaact aataagtcat
     1141 ttacttcatc cttatactca tcatacttag tcagctttag taatcccgga tataaaatgg
     1201 ccgacagctt gagtgtatca tataaatctg taaagaaatc agacatttca tcttcatcag
     1261 aaggtatagg tgcttcattt gtaaatatgc tatctataaa tttaaatcct ttactgtttt
     1321 tttgaagtgc aatcgcatcc aatacggcaa actgcaaatt gctggtgtca ccactttggg
     1381 catacatttt tatcaattta ggaagtacgc ttttatcctg aatcttgccg aattttataa
     1441 ttaagtcttc tctgtcagca acattgtatt tgtaataatt cggactttcc aggaaacgga
     1501 tgtagttagc cgcatcattt ttatctttaa atttcatcac atccaacgag gctatagctt
     1561 catcgcgctt gctgctgtct gcagagttta gggcgtcaaa aaacatcgca gactttgatg
     1621 tgagaggact atatcccagt agcgtatctt caggagtaaa tgaatcaaaa aaagttttca
     1681 caaattcact tggccgtcta tcgtatcaat aactgatgcc atgtagtaca agtatgcttt
     1741 gcgtaatact gttttcagta aaatgcctct ggtgctattg gtgtcggtaa gtacatagtc
     1801 aatgattgtt atctggtttt gttccttttt agagtaggaa gatattttca aaccgcattt
     1861 cttcgtataa tagtcaatgt cagtattcca gaaactgtca atattttgta tacttaaata
     1921 atcgtgcagt ttttcatacg taatatctat ctgttccttc gtggaaggat taaaataggt
     1981 tttgtttata atattgcttt catatggttt gatttcttct ttggcgctgt atttgttttg
     2041 ttgggtattg tatcctgcgt attctactaa ctcatctaat gctggtttta cacaggtttt
     2101 tacactgtaa tgtaatgcag tatcctgata ggtaaagaat ggggtgttgt atacctccgg
     2161 aacaaagtgg aaagaattaa aatattgttc cggctttttg ttggctgaac ttactgcact
     2221 caagaaataa taatgtgggc ctttgattat ggttttaata aagaggtatt taccttcttt
     2281 tgttttcagc gtggtttcaa aggcaggatg tttttcataa gtggaaaatt gtcggttgat
     2341 aacatcaaat ccccagtctt tattcattac ctcaatagga taagccagct caaaggtgtc
     2401 ttcttcaata aatttgtaat cactgtaatt tgctctcatg agcgaataat agatgccttc
     2461 tttgtcgtaa gcaaatgttt tatcaaaatg tgcgttgctg acaatatgtt ctgtttgctt
     2521 gtcaaatatc ctgtagtcgg gcaagcttgc cgtatagtcg ccatagtccg gggcataggt
     2581 actccactta tcgggctgta tattcagtga gatagaatta aagaaacggt cattctcttt
     2641 attgctaaaa taatcacccg cacccgctgc cttgaaaata aagatttcaa gcggtgtgat
     2701 gatgaaaata aatctacgga tatctcctcg ttgcgtttta gaagtaatat caaaacccgg
     2761 atagccaaac ttactgattt ctgttttctt taaaattttt ccgggtatat attcaaagaa
     2821 taaactgtca atgcgttgac gatttacatc aacgctataa ccacctaatt cgttatggga
     2881 tttgatgcga tataccgtat aataggcagc attagccgca tctatgcaca agtattgttt
     2941 caactcgtca aacacataaa tttcatacat attccccggc acgtctaccg agaaaatttt
     3001 gtcgtctgaa gtgaagtttt taaatgtgag tggcaatttt actttttcta atttatcttt
     3061 tgtctgtgta gccgtcttca ttttatccaa actaatcggt cttactgtat aaccctcttt
     3121 tcttagtaat tcaatcaaac cgtttttgcc gggtaaatgc cctgcgccaa ctcccgtaaa
     3181 aagagattgt ccggttttgt aaacctccat catactatgc agcatgacaa ggttccggtc
     3241 ttcaatcaga tatttgccat ataatttgga ggggtattgt ttgtgatcca aagaatcgag
     3301 catatccaga tcgccttttc tatatgcatc ttctactttc tctatgagtt tactttcaat
     3361 attatccaaa atattctcag attcatcttc catagaaatg atagagtcgt tctttttttc
     3421 tttctcttca tctctttcat tttgcggatt aatggctttg agataatatt tcatcgaacc
     3481 gtcaaaagtt tcgagtccaa tcgttttttt atttaatcgc ctcgcgcact ggaaaataaa
     3541 taaatcgaga taggtacttt cttcaaagtc actttgtcct ttatacaaat ccgaaaatcg
     3601 gtacatcatt tcattaataa tatcggggtc atcgcttatc aactcagcat aatcatttcg
     3661 ttcaggcact ttcatggaaa atgcatcctt atatagattt tctgttcctg attgattcat
     3721 caatgctgta gagagttgaa acatctttga cttaatcatt tcatccagat aaatatttaa
     3781 gtcagactca agagccacca catctgcacg ttgcatggca acaaaaaacg tatcgggcag
     3841 ataataagcc aatttcttgc tgacatgcat cgtgccgtac aaataagaag gcttgctcag
     3901 accgttacct gtaatttccc aaaagcggct ttggtatttc ttttgcgcat tcagcgtagt
     3961 gggcagcagt aaaagcaagt aagtaactac gaggataagt tttttattta tcatattaac
     4021 aaggtgatat ttagatttac gttcaattct ctaagtaaaa aattattcat ttgttttaga
     4081 ccatcaattg tcaaacttca gcattatata ttatcctgca attatactgt atacaacgta
     4141 aattattccc caagttcaag tgttaaatgc aattttttgg gggcataaaa taacattttt
     4201 tcaaaa
//
LOCUS       DAOQII010000228         4178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_12945_length_4178_cov_7.189910, whole genome shotgun sequence.
ACCESSION   DAOQII010000228 DAOQII010000000
VERSION     DAOQII010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12945_length_4178_cov_7.189910"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..3304
                     /locus_tag="PLF48_09865"
     CDS             182..3304
                     /locus_tag="PLF48_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNF49674.1"
                     /translation="MLKSSELIVYKASAGSGKTYALAREFLKIVIANPYDYKRILAVT
                     FTRKATEEMKSRIIENLAQLKNKDPKSQHLRKEIIHSLQPIFPDISMHIDNNAALALA
                     LILHDYSNFNISTIDSFFQSIIRAFAKELDLPIGMEIELDTQSALAVAVERVLKAYES
                     GKDEISQWIEDFVSDRIDEDKNWKIEDNLLQFSRELFNEDFRNIANEFALSDEKPDYK
                     KVVGQLKEIIRNYTAFFDYHLEEINEYIVQNQLDINGYKGRSTIRSFLNKLTDYNLEI
                     TKTLQDFLDTDIIFPAKTNLTASEKIKLEQAWHSVFKPFILECITHVTQHERQYISAK
                     AVLKNMYAFVLLDAINDELKKYKAEENLVLISDTNNIISLLSEKDAVPFIFEKSGSFI
                     KYILLDEFQDTSLLQWKGLSKLVLEILDNANGIVLIVGDPKQSIYRWRGGKMNLIIDG
                     IQQDLQHHWKQRNETPLNDNWRSAKEIVTFNNAFFKQLKAAKLLENDLYEAAYLDVEQ
                     NNKKQDIQGLVRFHFFDADNKKKLAEGEIPVKAKILEDLAKTITSFKQKKLSDIAILT
                     RTNTEGILVAQYLSEQHIPVVSAESLLLYSHRHIRLLIAALYYINFPDEQLYAVQLNY
                     LLAQHEGAEDIQNELKREPDFNYSFILHYPVFGRTIIHEQAQKDVWEIVQMLVSAFNL
                     LRNENEYVLRFQDIVLQHCSRYSNKLNEFLEFWETQKTRFAIMPPDGVNAVKIMTIHK
                     SKGLQFPVVILPFADWDIKPKPTQKIWIKSEVEPFNQLQAFSIETKKENLKSVFAAEI
                     QKETDAAFIDNINLLYVALTRAESELHVISVKPAGDPHELNKLNKIIYPVIQELNLDG
                     SHADADEFYFGKGDGIFPHDKKTWETEQLHVPSWSDYKKTLQLKQHDVFNKEQETGTQ
                     IHELLAENMHGNMSDILKNFATDERLSFEQLFTSIQFIFEQKGWNADKWQQLNERPIV
                     FEEQLLIPDKLLISDDEIIVLDYKTGVKNKKHHQQILTYIAVLGSMYKQPIKGYLLYL
                     KTMELETVSK"
     gene            complement(3297..3707)
                     /locus_tag="PLF48_09870"
     CDS             complement(3297..3707)
                     /locus_tag="PLF48_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF49675.1"
                     /translation="MRDKIELLQKIGQKIRLLRDMRKLSQENMADALHVSQKTYSNIE
                     KGSADIPISTLYNISEILEISVSNILEFDEENFIKNIFNNGNGNKGVNVMNQNIHNKD
                     FETNTLLLQTLQAEVDFLKEQNKQFIEMLKNKII"
     gene            3884..>4178
                     /locus_tag="PLF48_09875"
     CDS             3884..>4178
                     /locus_tag="PLF48_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49676.1"
                     /translation="MKRINLFSLIVIFTYLSCSKQEQITKESNQASSYITKPEDSIKK
                     LVTIIDETPDNEDNTSNWLNCNYYPPTDPRSCNYVVSDGEPTGISANATYANGM"
BASE COUNT         1448 a          712 c          740 g         1278 t
ORIGIN      
        1 tgatttctca ttttttgcct ccaactttat atggtgaaag aataatagag tggtggtttt
       61 tgataataaa atcaactaat tttatcccga actcaaattt atacaactag taatcataga
      121 gtttgcccct aagaaaatga caaattttaa gataactgat aatagtgtta atttagcctg
      181 catgttaaaa tcgtctgagc tgattgtata taaggcttct gcaggaagcg gaaaaacata
      241 tgcactcgcc cgcgagttcc tgaaaatcgt tatagccaat ccctatgatt ataaacgcat
      301 cttagcggtt acttttacgc gcaaggctac ggaagagatg aagtcgcgta ttattgaaaa
      361 tttggctcaa ctaaaaaata aagacccaaa atcacaacac cttcgcaagg agattatcca
      421 ctccctccaa ccgatttttc cggatatctc tatgcatatt gacaataatg ccgctcttgc
      481 gttagcgtta attttacacg attattccaa tttcaatatc agtaccatcg atagtttttt
      541 tcagtccatt attcgcgctt ttgccaaaga actggattta cctataggta tggaaataga
      601 actggacaca cagtcggcat tggctgttgc tgtagaacga gtgctcaaag catatgaatc
      661 aggaaaggat gaaatttcac aatggataga agacttcgtc agtgacagaa ttgatgaaga
      721 caaaaactgg aaaatagaag acaatttatt gcagttttca agagaacttt tcaatgagga
      781 tttcaggaat atcgccaatg aatttgcatt gagtgatgag aaaccggatt ataaaaaagt
      841 ggtaggtcag cttaaagaaa ttatccgaaa ttataccgca tttttcgatt atcatttgga
      901 agaaataaac gaatatattg tccaaaacca actcgatatt aacggataca aaggcagatc
      961 gacaatacgc tcttttctta ataaactaac agattataat cttgaaatta ccaaaaccct
     1021 gcaagacttt cttgatacgg atattatctt cccggctaaa accaacctta cagcatctga
     1081 aaagattaaa ctggaacagg catggcattc cgtttttaag ccctttattt tggagtgtat
     1141 aacccatgta acacaacatg agcgtcaata tatttctgct aaagccgtgc ttaagaatat
     1201 gtatgccttt gtgttattgg acgctattaa tgatgaactc aagaagtaca aagccgaaga
     1261 aaatctggtc ttaatttctg acacgaataa tattatcagc ttgctttctg aaaaagatgc
     1321 ggtgccgttt atttttgaaa aatcaggttc atttataaaa tacatattgc ttgatgaatt
     1381 tcaagatact tcattattac agtggaaagg attatctaaa ctggtgctcg aaattttaga
     1441 taatgcaaac ggtatcgtct tgatagtagg cgacccgaaa caaagtattt atcgctggcg
     1501 agggggtaag atgaatctga ttattgacgg cattcagcaa gatttacaac atcactggaa
     1561 gcaaagaaat gaaacccctt tgaacgacaa ctggcgcagt gcaaaagaaa tcgtaacttt
     1621 taataatgcc ttctttaagc aattgaaagc agcaaaattg ctggaaaatg atttatatga
     1681 ggcggcttat ctcgatgtgg aacaaaacaa caagaaacaa gatattcagg gcttggtgag
     1741 atttcatttt tttgatgctg acaataaaaa gaaactggct gaaggcgaaa ttccggtgaa
     1801 agccaaaata ttagaggatc tggcaaaaac tattacttca tttaaacaaa aaaaactaag
     1861 cgatattgcc atactcaccc gcacgaatac agaaggaatt ttagttgcac aatacttaag
     1921 tgaacaacat atacctgtag tgtctgctga atcattactg ctatatagtc ataggcatat
     1981 acgactcttg atagcggcat tgtattacat caattttcct gacgaacagc tttatgccgt
     2041 gcaattgaat tatttgcttg cacaacatga aggtgctgaa gatattcaaa atgagcttaa
     2101 acgtgaaccg gatttcaact attctttcat tttgcattat cctgtatttg ggcgcactat
     2161 tatacatgaa caggcacaaa aagatgtgtg ggaaattgtg caaatgctgg tttcagcttt
     2221 taatttattg agaaatgaga acgagtatgt gttgcgtttt caggatatcg tattacaaca
     2281 ttgcagccgc tactctaata agctgaatga gtttctggaa ttttgggaaa cacaaaaaac
     2341 ccgctttgcc attatgccac ccgatggggt gaatgcggtg aaaataatga ccatacacaa
     2401 atcaaaggga ttacagtttc ctgtagtcat actgcccttt gccgattggg acattaagcc
     2461 caaaccgacg caaaaaatat ggataaaaag cgaggttgaa ccattcaatc aactgcaagc
     2521 attttccata gaaacaaaaa aagaaaattt aaaatcagta tttgccgcag aaattcaaaa
     2581 agaaaccgat gccgctttta tcgataatat caatttgttg tatgtggcac ttacccgggc
     2641 tgagagtgaa ctgcatgtga taagtgtaaa gcctgccgga gatccgcatg aattgaataa
     2701 gctcaataaa attatttatc ctgttataca agaattaaat ttggacggca gtcatgctga
     2761 tgccgatgaa ttttattttg ggaaaggaga cggcattttt ccgcatgata aaaaaacttg
     2821 ggaaacggag cagttgcatg taccgtcttg gagtgattat aagaaaacat tgcagcttaa
     2881 acaacatgac gtctttaata aagaacagga aacaggcact cagatacacg aattacttgc
     2941 tgaaaatatg catggaaaca tgagtgacat tttgaaaaat ttcgctactg atgaacgttt
     3001 gtccttcgag caattgttta caagcattca atttattttt gagcaaaaag gctggaatgc
     3061 agacaaatgg caacagctta atgaacggcc tattgttttt gaagagcaat tattaatacc
     3121 cgataagctc ttgatttcgg atgatgaaat catcgtgctg gattacaaaa caggagtaaa
     3181 aaacaaaaaa catcatcagc aaatactaac ctatatagca gtattaggaa gcatgtataa
     3241 gcaacctata aaagggtatt tgctttattt aaaaaccatg gagctggaaa cagtatctaa
     3301 ataattttat tttttagcat ttcaataaat tgtttatttt gttcttttag aaagtctact
     3361 tcagcttgaa gagtctgtaa taaaagagta tttgtttcaa aatctttatt atgtatgttt
     3421 tggttcataa catttacccc tttatttcca ttgccattat taaaaatatt ttttataaaa
     3481 ttttcttcat caaattctag aatatttgaa acagaaatct ctaaaatctc actgatatta
     3541 taaagagtag aaatgggtat atccgcactt cctttttcaa tattagaata cgttttttga
     3601 gaaacatgta aagcatctgc catgttttcc tgagacaact ttctcatatc cctgagtagt
     3661 cttatttttt gaccaatttt ttgtaataac tctattttat ctctcatgta tcatgaattt
     3721 tttctatgta taaaaataga aaaatatttc catatttagt agctaaaaaa ttagattata
     3781 taccatacat ccatatacat ttgtagtata atagtgcacg aattatgggt ggcatttcga
     3841 aaaaccctta atagagtaga attaactttt taaccccctt aaaatgaaaa gaattaatct
     3901 tttttcacta attgttattt tcacttatct tagttgtagc aaacaagagc aaattactaa
     3961 ggaatcaaac caagcttctt catatatcac taagcctgaa gatagtatta agaaacttgt
     4021 aacaattatt gatgagacgc ctgataatga agacaatacc tccaattggt tgaattgcaa
     4081 ttattatcca cctaccgacc caagatcctg caattatgta gtctcagatg gtgagcctac
     4141 cgggatttcc gctaatgcaa catatgcaaa cggaatgt
//
LOCUS       DAOQII010000229         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13016_length_4161_cov_5.157574, whole genome shotgun sequence.
ACCESSION   DAOQII010000229 DAOQII010000000
VERSION     DAOQII010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13016_length_4161_cov_5.157574"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLF48_09880"
     CDS             <1..470
                     /locus_tag="PLF48_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49677.1"
                     /translation="NIDPNLLKVVGGSLDYGGSNLKENVSVAGVYSLSGAIVQPSVLQ
                     KSSKTPLLLIHGTQDDLVNPNVGAVFKCDGKNPTFPKVAGGNAIYEVMHTRVPIRLEL
                     VCKGGHGMGTVGYPKLLDLISTFTYSVLQGNPITGKEIIEAAVPVRQDNSLCK"
     gene            complement(471..1106)
                     /locus_tag="PLF48_09885"
     CDS             complement(471..1106)
                     /locus_tag="PLF48_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49678.1"
                     /translation="MRKFILILLLYGTHAASAQLFKTDTFAYSINLDASVNNYLKTLP
                     IDLLKGYCSGKWNAFYPKYEYNQCLWNDYLAHYQTANNNEQNLYCIDDYCSQPYFKAL
                     YDNFTRKIGYREIYYFDAKHSVIKRELLSIQLYYSFQDFKGEWIHVPSLIFWLKEINA
                     IPNAILISNKQIRWEAWTLEKEFATPGFIRNENPQKTNLQKETKIQDIEER"
     gene            complement(1108..2145)
                     /locus_tag="PLF48_09890"
     CDS             complement(1108..2145)
                     /locus_tag="PLF48_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49679.1"
                     /translation="MKTYRFILFAILLPLISFAKIPEQLTATAFTEYTFPSNTCKYID
                     HQGTKIFIQANSFYLGEKLYEGEVVFKFRQFTDQLDILLNHIPMTYASADNKYTLESG
                     GMFECYAYGNGKLLSFAPGKNMNIQLKTNFDIAGGELYKLNPTTKMWEKKTIFANNIN
                     ANENLPDSKADYWFDNFNNQEDAGFSGIQTNNTVAVVDPKTGNVVYELVQMTNADEIF
                     KTMNINEMGFYNCDKILDEQNAVDITVDFDLKGYNKKLASAVYVVYKNRNAVFTYYPD
                     KTTYSIKLLAGEECTIFSFGTDGKIALADQSKLMNTDLKNMKNIQLTIPMTVIKNTPN
                     SKAELAQLTGL"
     gene            2237..3025
                     /gene="argB"
                     /locus_tag="PLF48_09895"
     CDS             2237..3025
                     /gene="argB"
                     /locus_tag="PLF48_09895"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNF49680.1"
                     /translation="MKELVILKIGGNVIDDMDKCNLFLENFAQIKQAKILIHGGGKVA
                     TSLLTKLGIETKMIDGRRITDRQTVDIVTMVYAGLINKKIVAKLQAENCNAIGLSGAD
                     ANAVLAHKRVHPTIDYGFVGDIDSVNIELLNQFLQQSLTPVLAPITHDKQGNLLNTNA
                     DTMAAALAIAMAKYYKVTLIYAFEKNGLLKDVEDENSVIKLVNVKEVQQLKEKQIISG
                     GMIPKVDNIVYVLLHDVEKVILCKAENIADVLNGNPIFGTTFIK"
     gene            3025..4095
                     /locus_tag="PLF48_09900"
     CDS             3025..4095
                     /locus_tag="PLF48_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNF49681.1"
                     /translation="MQKAANETTEQIIALLKQLIAVESFSREEDKAAAVLRKFLKSHK
                     IAFETRQHNTWIKNKHYNFLKPTILLCSHIDTVKPVQNWTKNPFEAVEEDGKLYGLGS
                     NDAGASLVCLLGAFLHFYENEDLKYNLLYCAVAEEEVSGKHGMEMLEDITSACDFAIM
                     GEPTDMQMAIAEKGLLVLDGVIKGKSGHAARNEGNNAIYLAISDIVWFQNFQFKKINQ
                     LLGPVKNTVTVINAGTQHNVIPDICKYVVDVRTIPEYSTEQLLEIIRDNVKAEITPRS
                     VRLQPSQIDKEHILVKTAEKLGIELFGSSTLSDQALLKIPSVKIGPGKSERSHTADEF
                     VFLNEIELGLKGYVKLLQNICL"
BASE COUNT         1375 a          694 c          765 g         1327 t
ORIGIN      
        1 gaaatattga tccgaattta cttaaagttg tcggaggtag ccttgattat ggtggaagta
       61 atttaaaaga aaatgtaagt gttgccggtg tgtattcatt gtcgggtgca atcgttcaac
      121 caagtgtatt gcaaaaatca tcaaaaactc cattattact cattcatggt actcaagatg
      181 atcttgttaa tccaaatgtg ggagcggttt ttaaatgcga tggtaaaaat cctacttttc
      241 caaaagtagc aggtggtaac gctatatacg aagtaatgca taccagagta ccaatacgtc
      301 tagaattagt atgcaaaggt gggcatggca tgggtacagt tggctatcct aaattactgg
      361 atttaataag tacatttaca tacagtgtgt tacaaggcaa cccaataaca ggcaaagaaa
      421 ttattgaagc tgcagtacca gtacgtcaag ataattcact ttgtaagtaa ttaccgctcc
      481 tcgatatcct gtatttttgt ttctttttgt aggtttgttt tttgtgggtt ttcatttctg
      541 ataaatccgg gagtagcaaa ttctttttcc agtgtccaag cttcccatcg aatttgtttg
      601 ttgctgatta atattgcgtt tggaattgca ttaatttctt tcagccagaa tataaggctg
      661 ggcacatgta tccattctcc tttgaaatcc tgaaacgaat aatataattg aatggaaaga
      721 agctcacgct ttatgactga atgcttagca tcaaaataat aaatttctct gtagccgatt
      781 tttctggtaa aattatcata caatgcttta aagtaaggct gactgcaata atcatctatg
      841 caatatagat tttgttcatt attattggct gtttgataat gtgctaagta gtcattccac
      901 aggcattggt tatattcata tttaggataa aaggcattcc attttcctga acaatacccc
      961 tttaataaat caataggcag ggttttcaga taattattta cacttgcatc caaattaata
     1021 ctataagcaa aggtatctgt tttaaataat tgtgcagatg cagcatgagt gccatatagc
     1081 aatagtatca agataaactt tctcatttta caatccggtt agctgtgcca attctgcttt
     1141 agaatttggt gtatttttaa taactgtcat gggtatggtc agctgtatgt ttttcatgtt
     1201 ttttaaatcc gtattcatca gtttgctttg gtcagccaat gcaattttcc cgtctgttcc
     1261 gaatgaaaag atggtacatt cttcaccagc cagcaattta atagaatatg tagtcttatc
     1321 cggataataa gtgaatacgg cattcctgtt tttataaacc acatacaccg cagacgctaa
     1381 tttcttatta taacctttca aatcaaaatc tacagtaata tcaaccgcat tttgttcgtc
     1441 taagatttta tcgcaattgt aaaaccccat ttcatttata ttcatcgttt taaaaatctc
     1501 atcagcattt gtcatctgca ctaattcata taccacattg cccgttttag ggtcaacaac
     1561 agcaactgta ttgttagttt gaatacccga aaatccggca tcttcctgat tattaaaatt
     1621 atcaaaccag taatcggctt tgctgtcggg aagattttca tttgcattta tattattggc
     1681 aaatatagtt ttcttttccc acatttttgt agttggatta agcttataca actcccctcc
     1741 tgctatatca aaattagttt tcagctggat attcatattt tttccgggag caaaagaaag
     1801 taattttcca tttccatagg cataacattc aaacatgccg ccactctcca gtgtatactt
     1861 attatcagct gatgcataag tcatggggat atgattgagt aaaatgtcta actggtcggt
     1921 gaattgtctg aatttgaaaa cgacttcacc ttcatatagt ttctctccca aataaaaact
     1981 gtttgcctga ataaaaattt tggtgccttg gtggtcaata tatttgcagg tgtttgatgg
     2041 gaatgtatat tccgtaaaag cagttgctgt cagttgctcc ggtatttttg caaatgaaat
     2101 taaaggcaat aaaatagcga ataaaataaa acgataggtt ttcatagtta gcatttgtaa
     2161 tagaacgtca ggcgttaaaa aatattgtta cttaaaaata caaattttat cattctgatt
     2221 catgtaaatt tgcataatga aagaactggt aattctgaaa atcggcggta atgtaataga
     2281 cgatatggat aaatgcaatc tctttttaga aaattttgcc caaataaaac aagctaaaat
     2341 tttaattcat ggtggcggaa aagtggcaac ttcattgctg accaaactgg gtattgaaac
     2401 aaaaatgatt gacggcagga gaattactga ccggcaaacc gttgatattg ttaccatggt
     2461 atatgcaggt ttgattaata aaaaaattgt cgccaagctg caagctgaaa attgtaatgc
     2521 catcggatta agcggcgctg atgctaatgc cgtactagca cataaacgtg tccatccaac
     2581 aatagattac ggctttgtcg gagatattga cagcgtaaat attgaattat taaatcagtt
     2641 tttacaacaa tcgctaacac cggtattagc acccattaca cacgacaaac agggaaattt
     2701 gctgaatacc aatgccgata caatggccgc agcgttggct attgctatgg ctaaatatta
     2761 taaggttact ctcatttatg cctttgaaaa aaacggatta ttgaaagatg tcgaagatga
     2821 aaacagtgta attaagctcg tcaatgtaaa ggaagtacag caactcaaag agaaacaaat
     2881 cattagtggg ggcatgattc caaaagtgga taacattgtg tatgttttat tgcatgatgt
     2941 ggaaaaagta atattatgca aagctgaaaa tatagcggac gttttaaacg gaaatcctat
     3001 ttttggcact acatttataa aataatgcaa aaggcagcga atgaaacaac agaacaaata
     3061 attgctttac tgaagcaact aattgctgtt gaatcattca gcagagaaga agataaagca
     3121 gctgcggttt tgcgtaaatt tttgaagtca cataaaattg catttgaaac cagacaacac
     3181 aatacctgga taaaaaacaa gcattataac tttttaaaac ccaccatctt actttgttct
     3241 catatcgata ccgtgaagcc tgtacaaaac tggactaaaa atccgtttga agccgtagaa
     3301 gaagatggta aattatatgg tttgggaagc aatgatgccg gtgcttcatt ggtctgctta
     3361 ttaggtgcat ttttgcattt ttatgaaaat gaagatctca aatacaactt gctctattgc
     3421 gcagtggcag aagaagaagt atcgggaaaa catggtatgg aaatgctgga agatataaca
     3481 tcagcttgcg attttgctat catgggcgag cctaccgaca tgcaaatggc cattgcagaa
     3541 aagggtttat tggtgctgga tggtgtgata aaaggaaaat ccggacatgc tgccagaaat
     3601 gaaggaaata atgcgatata tctcgcaata tcagatatcg tttggttcca aaatttccaa
     3661 ttcaaaaaga ttaatcaact attgggtcct gtaaaaaata cggttactgt catcaatgca
     3721 ggtacacaac acaatgttat tccggatata tgtaaatatg tggttgatgt tcgcactatt
     3781 ccggagtaca gtactgaaca attactggaa ataatccgcg ataatgtgaa agcagaaata
     3841 actccacgct cagttcgttt acaaccctca caaattgata aggagcatat cttagtaaaa
     3901 actgctgaaa aattaggcat tgagttattt ggctcatcca ccttatccga tcaagctcta
     3961 ttaaaaatac ctagtgtcaa aatcggccca ggcaaatcag aacgttcaca tacagcagat
     4021 gaatttgttt ttctgaatga aattgagttg ggattaaaag gctatgtcaa actattgcaa
     4081 aatatttgct tataatttta tacaactaat aacctgagtt cgggataagc gtttcaatta
     4141 aaaaatagtt gattttttat a
//
LOCUS       DAOQII010000230         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13047_length_4156_cov_5.304316, whole genome shotgun sequence.
ACCESSION   DAOQII010000230 DAOQII010000000
VERSION     DAOQII010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13047_length_4156_cov_5.304316"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..359)
                     /locus_tag="PLF48_09905"
     CDS             complement(54..359)
                     /locus_tag="PLF48_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49682.1"
                     /translation="MNKFIQRYTGFFRNMRWVHFLYNVFHKKGLNHNLHLYPYYNLKK
                     SIFSSISHKDFEGKRAKLPWLDTINSGKDIENHPVFCHFPKSIEEQLLGWHKNGYLV"
     gene            458..649
                     /locus_tag="PLF48_09910"
     CDS             458..649
                     /locus_tag="PLF48_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49683.1"
                     /translation="MKKPEQQKTFPKNNSKKHIVSNFNTKETPLTLPIDYSNATESLM
                     PELEELIKKNPNILIGCGG"
     gene            674..1237
                     /locus_tag="PLF48_09915"
     CDS             674..1237
                     /locus_tag="PLF48_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNF49684.1"
                     /translation="MNKLYIKNMVCNRCITAVRQELDKLQFQIKSITLGEVLLENDIT
                     ETEKNKIENVLVPLGFELIDDKKSRIIEQIKNKIIEIVHYQNNAAKYNLSEILSKETH
                     HEYNYLSNLFSEIEGTTIEKYFIAQKIEKVKELILYDELSLSEIADRLNYSSVAYLSN
                     QFKKVTGLTPSFFKQLKTVQRKPLDEV"
     gene            1316..3523
                     /locus_tag="PLF48_09920"
     CDS             1316..3523
                     /locus_tag="PLF48_09920"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNF49685.1"
                     /translation="MPTILKKSFPVLNMSCASCAVSAESIVKSTEGVVSASVNYANAM
                     LTVEYQPAITNPSAIQHAVQSVGYDLILEEDEQQQETVAALYEKKYQALKTKTIWAVL
                     LALPVMLIGMFFMHVKYANEVMWLLSTPVVLWFGKDFFINAWKQAQHRSANMDTLVAL
                     STGIAYLFSLFNLFFPEFWLKRGLEPHVYFEAAAVVIAFILVGKLLEEKAKGNTSAAI
                     KKLMGLQPKTVTVVQADGSELQTTIENVKIGDLLIVKPGERIAVDGIVSSGSSYVDES
                     MLTGEPIPVLKVAAEKVFAGTINQKGSFQFAATKIGKDTLLAHIIKMVQDAQGSKAPI
                     QKLVDKIAAIFVPTVMGFAVATFLLWVILGGENALIHGLLTAVSVLVIACPCALGLAT
                     PTAIMVGVGKGAESGILIKDAESLEWTKKINAVVLDKTGTITEGKPQVTDIQWLNNDS
                     TDRSILYSIEKKSEHPLAEAVVNYIKEEKPITLSSFESITGKGIRATYTNENYWVGTK
                     KLLLENHCSIDAELLQFAEQWSAQAKTVIWFADAKKALAVLAIADKIKDTSAKAIQQL
                     QAENIEVYMLTGDTMPTAATIARQTGIQHYQAEMLPQQKADFIKKLQQQGKIVAMVGD
                     GINDSTALATADVSIAMGKGSDIAMDVAKMTIVSSDLMKISQAIRLSKQTVATIKQNL
                     FWAFIYNLIGIPIAAGILFPINGFLLNPMIAGAAMAMSSVSVVSNSLRLKWKK"
     gene            3625..>4156
                     /locus_tag="PLF48_09925"
     CDS             3625..>4156
                     /locus_tag="PLF48_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF49686.1"
                     /translation="METGKINVQTENIFPIIKKFLYSDHEIFLRELVSNAVDATKKLK
                     GLAQLGEAKGELGDLTIDVSVNKDKNTLTIKDKGLGMTADEVKKYINQIAFSSAGEFV
                     EKLKNTDSNAIIGHFGLGFYSAFMVAEKVEILTKTYKDEPAVKWVCDGSTEFTLEEIE
                     KEQRGTEIILHIAEDSK"
BASE COUNT         1512 a          726 c          780 g         1138 t
ORIGIN      
        1 tcatcaattt cttggttaat ggtatcaaca gtctcgtcgt ctaaaaactg ctctcatacc
       61 aagtatccgt ttttatgcca tcccaacaat tgctcttcaa tggattttgg aaaatggcaa
      121 aataccggat gattctcaat atccttccct gagttaatcg tatctaacca cggcaattta
      181 gcccgtttgc cctcaaaatc cttatgcgaa atactactga aaattgactt ttttaaatta
      241 taatacggat ataaatgcaa gttgtgattt agaccctttt tatgaaatac attgtataaa
      301 aaatgtaccc aacgcatgtt gcgaaaaaaa ccggtatagc gttgtataaa tttattcata
      361 ctcgttaggc tcgtgttcaa aaatacatca caaatataaa ctctattcaa agataaacgt
      421 tattgattat tataaggata aatgattaaa tttaagtatg aaaaaacctg aacaacaaaa
      481 gacatttcca aagaacaata gtaaaaagca tattgtaagt aattttaata ctaaggaaac
      541 accactcaca ctgcctatag attatagtaa tgctacagaa agtttaatgc cggagttaga
      601 agagttaata aagaaaaatc cgaatatatt aatcgggtgt ggcggataaa tgtacaatag
      661 aaatgatgca caaatgaata aattatacat aaaaaatatg gtgtgcaatc gttgtattac
      721 tgctgtacga caagagttag acaaactaca atttcaaata aaaagtataa ccttgggtga
      781 agtactactt gaaaatgaca tcacggaaac agaaaaaaat aaaatagaaa atgtattagt
      841 tcctcttgga tttgaattga ttgacgataa aaaaagcaga attatagagc agataaaaaa
      901 taaaattatc gaaattgttc actatcaaaa taatgctgct aaatataatt tatcggaaat
      961 attaagcaaa gaaacccacc acgaatacaa ttatctttca aatctatttt ctgaaataga
     1021 aggcactaca atagaaaaat attttatagc acaaaaaatt gaaaaggtaa aagaactcat
     1081 cctatacgat gaactttcac tcagtgaaat agccgaccga ttgaattact ccagtgttgc
     1141 ttatttaagc aatcagttta aaaaagtaac aggacttacc cccagttttt ttaaacaatt
     1201 aaaaacggtt cagcgaaaac cattggacga agtgtaaatc ttataaatca tatccaaaat
     1261 actgtaataa tattccagca atgcattcgt attttcgtgt attaaaacca gcagtatgcc
     1321 taccatccta aaaaaatctt ttcccgtatt aaatatgtcg tgtgcttctt gtgcggtaag
     1381 tgcagaaagt atagtaaaat ctaccgaagg cgttgtttct gcttctgtaa attacgccaa
     1441 tgcgatgctc actgtagaat atcagccggc tattaccaat ccatccgcca tccaacatgc
     1501 agtacaatcg gttggctatg atttgatcct agaggaagac gaacaacaac aagaaacagt
     1561 agcggcatta tacgaaaaaa aatatcaggc attaaaaaca aaaacaatat gggcggtttt
     1621 gcttgccctt ccggtgatgt tgattggtat gttctttatg catgtaaaat atgcaaacga
     1681 agtaatgtgg ctgttatcca cgccggtagt gctatggttc ggaaaagatt tttttatcaa
     1741 tgcttggaag caagctcaac atcgatctgc taatatggat acgttggttg cattaagcac
     1801 aggtattgcc tatcttttta gtttattcaa tttgtttttt cctgaatttt ggctcaaaag
     1861 aggtttagag cctcatgtat attttgaagc agcggcagta gttattgctt ttattttagt
     1921 aggaaaatta ttggaagaaa aagcaaaagg aaatacctcc gcagccataa aaaaactgat
     1981 gggattgcaa cccaaaactg taactgtagt acaagctgac ggcagcgagc tgcaaaccac
     2041 aatagaaaat gtaaaaattg gagatttatt aattgtaaaa cccggtgaac gcattgccgt
     2101 agatggaatt gtaagcagtg gaagctctta tgtagatgag agtatgctca ccggagagcc
     2161 tattccggtt ttgaaagtag cagcagaaaa agtatttgcc ggcaccatca atcaaaaggg
     2221 aagctttcag tttgccgcaa caaaaatagg caaagatacc ctactggcac acattataaa
     2281 aatggtgcaa gatgcacaag gcagcaaagc accgatacag aaattagtag ataaaattgc
     2341 cgctattttt gtaccgacag tgatgggatt tgccgtcgct acctttttac tttgggtaat
     2401 tttaggaggc gaaaatgcat taatccatgg gctcttaact gccgtttctg tattagtaat
     2461 tgcctgccct tgtgcgctcg gattagctac gcccacagca ataatggtag gggtaggaaa
     2521 aggggcagaa agtggaattt taataaaaga tgcagaaagt ttggaatgga caaaaaaaat
     2581 aaatgcagtc gtattagata aaaccggtac cattaccgaa ggaaaaccac aagtaaccga
     2641 tatacaatgg ctgaataacg atagcacaga ccgttcaata ttatacagca tagaaaaaaa
     2701 atccgaacac cccttagcgg aggcggtggt taattatatt aaggaggaaa aaccaattac
     2761 gttatcttct tttgaaagta ttaccggaaa aggaattcga gccacttata ctaatgaaaa
     2821 ttattgggtt ggaactaaaa agctgctttt agaaaaccat tgcagcatag atgccgagtt
     2881 attgcaattc gctgaacagt ggagtgcaca agcgaaaaca gtaatctggt ttgccgatgc
     2941 aaaaaaagca cttgcagtac ttgcaattgc cgataaaata aaagacacct ctgccaaagc
     3001 catccaacaa ttacaagcag aaaatataga agtgtatatg cttaccggcg ataccatgcc
     3061 caccgctgca acaatagccc ggcaaaccgg catacaacac tatcaggcag aaatgctgcc
     3121 gcagcaaaag gcagatttta taaaaaaatt acaacagcaa ggaaaaattg tagccatggt
     3181 aggcgatggt ataaacgaca gtaccgcatt agccaccgcc gatgtaagta ttgctatggg
     3241 caaaggttcg gatattgcca tggacgttgc caaaatgacg attgtttctt ctgatttaat
     3301 gaagatttcg caagccatcc gactgtccaa gcaaactgtt gcgaccatca aacaaaatct
     3361 gttttgggca tttatctata acctcatcgg aatacctatc gccgcaggta ttttatttcc
     3421 tataaatgga tttttattaa acccgatgat tgccggtgcg gcaatggcaa tgagtagcgt
     3481 cagcgtagtg agcaacagtc ttaggttaaa atggaaaaaa taattccata caaaaatgtc
     3541 aacttgtcag caaaattcaa tcggtacaac ttttgacttc ttatgaaaaa tcttaattct
     3601 taaattataa atcttaattt ctttatggaa accggaaaaa ttaatgtaca aacagagaac
     3661 atctttccaa tcatcaaaaa attcttatac tctgaccacg aaatattttt acgtgaatta
     3721 gtttcaaatg ccgtagatgc tactaaaaaa ctgaaaggac ttgcccaatt aggcgaagca
     3781 aaaggagaac tcggcgattt aacgatagac gtatcggtaa ataaagacaa aaatacgttg
     3841 accattaaag ataaaggatt gggaatgaca gccgatgagg tgaaaaaata catcaaccag
     3901 attgcttttt cttcggcagg cgaatttgta gaaaaattaa aaaatacaga ttctaatgcc
     3961 attatcgggc atttcgggtt agggttttat tcggcattta tggtggctga aaaagtggaa
     4021 attttgacca aaacatataa agacgaacct gccgtaaaat gggtgtgtga tggctctacc
     4081 gaatttactt tagaagaaat agaaaaagaa caacgcggca cagaaatcat tttacacatt
     4141 gccgaggatt ctaagg
//
LOCUS       DAOQII010000231         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13076_length_4149_cov_6.118955, whole genome shotgun sequence.
ACCESSION   DAOQII010000231 DAOQII010000000
VERSION     DAOQII010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13076_length_4149_cov_6.118955"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLF48_09930"
     CDS             <1..321
                     /locus_tag="PLF48_09930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNF49687.1"
                     /translation="ELYYADLNPVKGTEQGGLRPVLVISGNTLNTYMDICIICPLTTK
                     IKNFHGCLVLKKNKENKLKTDSEVLTFQIRTISKDRLVKKIGNITAKELEQIRVYLNE
                     ILTL"
     gene            325..648
                     /locus_tag="PLF48_09935"
     CDS             325..648
                     /locus_tag="PLF48_09935"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNF49688.1"
                     /translation="MHWYPLSNEIKQAFLQLKMQQLAKFTYQEKDICVVRLEDGWHGI
                     SNRCPHAGASLHHGHCNKKGIVSCPLHGYKFDIKTGLSADGNNYKIQRYNFKLENDIL
                     YIGIK"
     gene            656..3133
                     /locus_tag="PLF48_09940"
     CDS             656..3133
                     /locus_tag="PLF48_09940"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNF49689.1"
                     /translation="MNYTLTEIAEIVNGNISITHIQTKLTQVCIDTRQISNPASSLFF
                     CLVGKADGHDFIATAYQKGIRHFVISKEINTADVQDASFILVKDTLVALQQLATYHRQ
                     QFSIPVVGITGSNGKTIVKEWLYQLLSDELNVVKSPKSYNSKIGVPLSVLNLQPEHQF
                     AIFEAGVSTVNEMDVLQKIIQPTLGIFTNIGSAHNSGFSDLTQKTKEKLNLFSSCTKV
                     IYTSKYAEIKDAVIGNPVYISWGFRSTDDYVITHFVKEKTKSTLQLNGISYTLPFTDD
                     ASVENAIHCIVFLKELNYTDENIQAKINALHNLPMRLELKYGINNCSIIDDSYSADMH
                     SFKVALDFLNQQESNKSRTLIISEFEESGLTEDAFIKQLIRIIQEYRFSKLIGIGPFF
                     KKAIESINGKVKEWYIYETTEQLISQFHLQHFENEFILLKGARRFGFEKISALLVGQT
                     HETVLEVNLNALLNNLNVYKAYLPTNCGVIAMVKAFSYGSGTVEVASLLEKNHVDYLA
                     VAYADEGVVLRKSGIRTPIMVMNPDVKDFDRMLEYQLEPEIYSVYILQQLIDFFSNRK
                     EEMKPFAVHLKIETGMNRLGLKQQDLTEFIEVLKTNKAIEVKTIFSHLSGSEDAQFDE
                     FTQQQIDLFQSFSQQISHELKQVYGNLYTEPKRHILNSAGIIRFPDARFDYVRLGIGL
                     YGVDGSQNIQDKLLPIGRLKTRIAQIKIVEKGETIGYSRKGIANETKKIAVLAIGYAD
                     GYDRRFGNGVGEVFISGKRAPIIGNVCMDMCMADITHIDEVKEGDDAEIYGSHISIIE
                     QAKKIGTIPYELLTSISPRVKRLYFLD"
     gene            3395..3844
                     /locus_tag="PLF48_09945"
     CDS             3395..3844
                     /locus_tag="PLF48_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF49690.1"
                     /translation="MNENQNLIHKVCRIYCRDKEAHNDLFQEIVLQLWKSFPTFRGES
                     KLSTWMYRIALNTTISGFRKKRITTTELSSISYQLQDIATPLQEEEMKALYAAIEKLT
                     DVEKSIVLLYLEDKPYDEIADITGITANYVAVKMNRIKEKLRKLIKI"
     gene            3875..>4149
                     /locus_tag="PLF48_09950"
     CDS             3875..>4149
                     /locus_tag="PLF48_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49691.1"
                     /translation="MWQKSDPVFTHEDINRQELAVMIRQRSVSIVERMKRNVFTEIIV
                     SCFCMLLIGYIPFIYRNATISIITVVVLLFVFVPYLIYYTRKYGELKR"
BASE COUNT         1389 a          700 c          796 g         1264 t
ORIGIN      
        1 gaattgtatt atgcagattt gaatccggta aaaggcactg agcagggtgg cttaagaccg
       61 gtgttggtga ttagcggaaa taccctgaat acctatatgg atatttgtat aatttgccct
      121 ttaacaacta aaatcaagaa ctttcatgga tgcttggtct tgaagaaaaa taaagaaaat
      181 aaacttaaaa cagattctga agtgctgact ttccaaatcc gcactatctc caaagaccga
      241 ttagtaaaaa aaataggaaa tattacagct aaggaattag aacaaatcag ggtatatctg
      301 aatgaaattt taaccttgta acacatgcac tggtatccgc tttctaacga aataaaacaa
      361 gcttttttgc agcttaaaat gcagcaactt gccaaattta cctatcagga gaaagacatt
      421 tgtgtagtgc gtctggaaga cggatggcat ggtatatcca acagatgccc acatgccggc
      481 gcttctttgc atcatggaca ttgcaataaa aaaggcattg tttcttgtcc gctgcatggc
      541 tataaatttg atatcaaaac aggattaagt gcagacggga ataactataa aattcaacga
      601 tataatttta aattagagaa tgatattttg tatatcggca ttaagtaatt tatacatgaa
      661 ctacacactt acagaaattg cagaaatagt aaatggcaac atttcaataa ctcatattca
      721 aacaaaactt acccaagttt gtatagacac ccgacagatt tccaatcctg cttcatcttt
      781 gtttttttgt ttggtaggaa aagcagacgg tcatgatttt attgctacag cttatcaaaa
      841 aggcatacgc cattttgtta tttccaaaga aataaatact gctgatgtgc aggatgcgag
      901 ttttattttg gtaaaagata cattagttgc acttcaacaa ttggcaactt atcacagaca
      961 gcaattttct attcctgttg taggtattac cggcagtaat ggcaaaacta ttgtaaaaga
     1021 atggctgtat cagttattgt ctgatgaact taatgtggtg aaaagcccta aaagttataa
     1081 ctctaaaata ggagtgcctt tgtctgtgct caaccttcag cccgaacatc agtttgctat
     1141 ttttgaagcc ggtgtttcaa ctgtaaacga gatggatgta ttgcaaaaaa ttatacaacc
     1201 cacactcggt atttttacga atatcggttc ggcacacaat tccggttttt ctgatttaac
     1261 tcaaaaaaca aaagaaaaac tcaacttgtt ttcatcttgt actaaggtga tttatacttc
     1321 taaatatgct gaaataaaag atgcagtaat tggcaatcct gtttatattt cctggggttt
     1381 tcggtcaaca gatgattatg taattaccca ttttgttaaa gaaaaaacga aatctacatt
     1441 gcaattgaat ggcatctctt acacccttcc atttacagac gatgcctctg ttgaaaatgc
     1501 gattcattgt attgtttttc tgaaagaact aaactatact gatgaaaaca ttcaagctaa
     1561 aatcaatgcg ttgcataatc tcccgatgcg tttggaatta aaatacggca ttaataattg
     1621 ctcgattatt gatgattctt acagcgcaga tatgcattcg tttaaagtag cattagactt
     1681 tctcaatcag caagaatcca ataaaagccg cacactcatc atctcggaat ttgaagaatc
     1741 gggcttgacg gaagatgcat ttatcaaaca acttattcgc atcatacaag aatatcgatt
     1801 ctccaaatta attggaatag gtccgttttt taaaaaagcc attgagtcta taaatggtaa
     1861 agtaaaagaa tggtatatct acgaaacaac agaacaattg atcagtcagt ttcatttgca
     1921 gcatttcgaa aatgaattta ttctgttaaa aggtgcacgc cgatttggct ttgaaaaaat
     1981 atctgcctta ttagtcggac aaacgcacga aaccgtgctt gaagtcaacc tgaatgcact
     2041 gctgaataac ttgaatgttt acaaagcata tctgccgaca aattgcggtg ttattgccat
     2101 ggttaaagca ttttcgtatg gcagcggtac cgtggaagta gctagtttat tagagaaaaa
     2161 tcatgtagat tatttagcgg tggcctatgc agatgaaggt gttgtattgc gtaaaagcgg
     2221 catccgtaca ccgattatgg tgatgaatcc ggatgtaaaa gattttgacc gcatgctgga
     2281 atatcaactt gagcccgaaa tttattcggt gtatattttg cagcaattaa tcgatttttt
     2341 tagtaaccga aaagaggaga tgaagccctt tgccgtgcat ctgaaaatcg aaaccggtat
     2401 gaatcgattg ggattaaaac agcaagattt aactgagttt attgaagtgc tgaaaacaaa
     2461 taaggctata gaagtaaaaa ccattttctc ccatctcagc ggaagtgaag atgcgcagtt
     2521 tgatgaattt acacaacaac aaatagattt atttcagtcc ttttcacaac aaattagcca
     2581 tgaactaaaa caagtatatg gaaatttata tacagaacca aaacggcata tcttaaactc
     2641 ggcaggtatt atccgtttcc ccgatgcaag atttgattat gtgcgtttgg gaataggttt
     2701 atatggtgta gacggcagtc aaaacattca ggataaatta ttgcctatcg gtaggttgaa
     2761 aacacgtatt gcacaaatca aaatcgtcga aaaaggagaa acgattggct actcgcgcaa
     2821 aggaatagca aacgaaacaa aaaaaattgc cgtgcttgcc attggttatg ccgacggcta
     2881 cgaccgccgt ttcggtaatg gcgttggcga ggtttttatt tcaggcaaaa gggcaccgat
     2941 tatcggcaat gtctgtatgg atatgtgtat ggctgatatc acgcatattg acgaagtgaa
     3001 agaaggcgat gatgccgaaa tttatggcag tcatatttct atcattgagc aagctaaaaa
     3061 gattggtact attccttatg aattgctcac gagtatatct ccgagagtaa aaagattata
     3121 ctttttagat taattgtgcc gtcagaagag aattactgac ataacgcaaa ttgtaagcat
     3181 gcttacaact atatgctgct attttggtct tttgttttac tatagatttc atttactgtt
     3241 tctatctctc tatgtggtta acgattccaa attctattca aaaaaatgtt ctgtatgcat
     3301 cgtgttgcta agtttttgtt gttgtctgta atattttgct gaactttact acttatataa
     3361 aaacacattt ctggagcagc agtttttaaa ggtcattaat gaaaatcaaa acctgattca
     3421 caaggtatgt cgaatctatt gcagagataa agaagcgcac aatgatttgt ttcaggagat
     3481 tgtattacag ttgtggaagt cgtttcctac tttcaggggc gaatccaaat tgagtacttg
     3541 gatgtaccgc atagccttaa acacgaccat atcaggattc agaaaaaagc gcattacaac
     3601 tacagaatta agcagtatca gttaccagtt gcaggatata gcaacaccac ttcaagaaga
     3661 agaaatgaaa gccttgtatg ccgccataga gaaattgact gatgtcgaga aatcaattgt
     3721 attgctttat ctcgaagata aaccttatga tgaaattgcg gatattacgg gtataaccgc
     3781 caattatgta gccgtaaaaa tgaacaggat aaaagaaaaa ttacgtaaac tgataaagat
     3841 ataatatatg gttgaattag aggaattaaa atcaatgtgg cagaaatcgg atccggtttt
     3901 tacacatgaa gatataaatc ggcaagaatt ggctgtaatg attcgccaac gctccgtatc
     3961 tattgtcgaa aggatgaagc gcaatgtgtt tacagaaata atcgtttcct gtttttgtat
     4021 gctgctgata ggttatatcc cttttatcta tcgcaatgct acgataagca taattacagt
     4081 agtcgttttg ctatttgtct ttgttccgta tctgatttac tatacccgga aatatggaga
     4141 acttaaacg
//
LOCUS       DAOQII010000232         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13489_length_4059_cov_4.589910, whole genome shotgun sequence.
ACCESSION   DAOQII010000232 DAOQII010000000
VERSION     DAOQII010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13489_length_4059_cov_4.589910"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLF48_09955"
     CDS             <1..1220
                     /locus_tag="PLF48_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNF49692.1"
                     /translation="IANENNLPCINLVESAGANLPDQAQVFNLGGNNFKEITRRSKNG
                     IPTISIVFGNSTAGGAYIPGMSDYTIFVKNQAKVFLAGPPLVKMATNEIVDDETLGGA
                     EMHSKVSGVSDYLAEDELDGIRIARELMANLNNLQSNSEFQPSTKYKEPLYNIDELLG
                     IISTDVKIPFDCREVIARIVDGSEFHEFKKDYGSTLICGFAKIHGYPIALISNNGVLF
                     NDSANKGAHFIQLCNMNKTPILFLQNITGFMVGKDYERNGIIKDGAKMINAVSNSEVP
                     MFTIMMAASYGAGNYAMCGRAYNPRFIFSYPNSMIAVMGAEQLAGVMQMVAKEGALKS
                     GKPWDENQAEQMKAFMKLEIEKQSNAFFATGQLWDDGIIDPRDTRHVMGLILALTYLQ
                     NVKGTNAWGVYRM"
     gene            complement(1255..1647)
                     /locus_tag="PLF48_09960"
     CDS             complement(1255..1647)
                     /locus_tag="PLF48_09960"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNF49693.1"
                     /translation="MAKKVPFTVERTFRSSPAILYSFLTTPSGLVQWFADYVDLDPNG
                     EVFTFEWNGEEQNAEVLAYEEEEFVKLRWEDDDDDRAYFEYRIRVTDITKETILTVTD
                     YAYKEDVESSKALWMSQLNDLAKALGNN"
     gene            1888..2154
                     /gene="rpsO"
                     /locus_tag="PLF48_09965"
     CDS             1888..2154
                     /gene="rpsO"
                     /locus_tag="PLF48_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNF49694.1"
                     /translation="MLSKEKKLEIFKEFGGEATNTGSVESQVALLTNRINHISGHLKT
                     NRKDFSTTRSLYKLVGQRKKLLKYLANKDIVRYRALLDKLGLRK"
     gene            2224..>4059
                     /locus_tag="PLF48_09970"
     CDS             2224..>4059
                     /locus_tag="PLF48_09970"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNF49695.1"
                     /translation="MQFNIHTQIVDYGDGRTVTVETGKLARQADGAVTVRCGNCIILA
                     TACASEEPKVGIDFFPLTVEYQEKFASAGRIPGSFMRREGKLSDHEVLISRLVDRAIR
                     PMFADNFKNDTQVLIYLISNDDNVDPDTLVGLAASSALSVSSIPFEGPISEVRVVKAN
                     GQFIVNPYRNVKDSLELDIILAGTAENILMVEGEAKECSEADLVEAIKLGHEIIKKQC
                     TAQLELIQKAGKTKMEVTAPEEDEELQAKITAFAKDRILTIAKSALGKHERKTGLKQI
                     KADFIDAIPEEELETFNKDLFSKYYAKLEKETIREMILNEKMRLDGRKTDEIRPLFME
                     VDVLPSPHGSALFTRGETQSLTTVTLGSKQDEQLIDKAEDLSYNKFLLHYNFPPFSTG
                     EVKPMRGTSRREVGHGNLAMRSLKQMLPAEESNPYTLRVVSDILESNGSSSMATVCAG
                     TLALMDAGVQIKKHVSGIAMGMISKDEKYAILSDILGDEDHLGDMDFKVTGTRDGICG
                     VQMDLKIDGLSYVVLAEALEQARKGRLHIINAMEICIPAARPDIKPHAPRVENIIIPK
                     EFIGAVIGPGGKVIQEMQEVTNTTITIEENKEKGIGNVIIFANNKE"
BASE COUNT         1396 a          735 c          796 g         1132 t
ORIGIN      
        1 aaattgccaa tgaaaacaat ctgccatgta tcaatctcgt ggaaagtgct ggagcaaacc
       61 tccccgatca ggcacaagta tttaatttag gaggcaataa ttttaaagaa attacccgcc
      121 gatctaaaaa tggcatacca actatttcta ttgtgtttgg caactctact gcaggcggcg
      181 cttatatccc gggcatgagt gactatacca tatttgtaaa aaatcaagca aaagtatttt
      241 tagcggggcc accattggta aaaatggcta caaatgagat tgttgatgac gaaacattag
      301 gaggtgctga aatgcattcc aaagtttcag gagtatcaga ttatttagcc gaagatgaat
      361 tagacggcat acggatagct cgagagctga tggcaaattt gaataactta caatcaaatt
      421 ccgaattcca gccctcaacc aaatataaag aaccgcttta taatattgat gaattacttg
      481 gaatcatttc tactgatgta aaaataccat ttgattgtcg ggaagtaatt gcaagaattg
      541 ttgacggttc agaatttcac gaattcaaaa aagattacgg ctcaacgctc atttgtggtt
      601 ttgcaaaaat acatggatat cctattgctt taattagtaa taatggtgtc ttattcaatg
      661 attctgccaa taaaggagcc catttcatcc aactttgcaa catgaataaa acgccgattt
      721 tgtttttaca aaatataaca ggattcatgg taggaaaaga ttatgagcgc aatggcatca
      781 taaaagatgg tgcgaaaatg ataaatgccg tttctaattc tgaagttccg atgttcacga
      841 tcatgatggc tgcctcttat ggtgcaggca attacgccat gtgtgggcga gcctacaacc
      901 ctcgatttat attctcttat cctaatagca tgatagctgt aatgggcgct gaacaactgg
      961 caggcgtgat gcagatggta gcgaaagaag gcgcactaaa aagtggaaag ccctgggatg
     1021 aaaatcaggc tgaacaaatg aaagccttta tgaaattaga aattgaaaaa caaagcaatg
     1081 cattttttgc aaccggacag ctgtgggatg atggtatcat agatccacgt gatacccgtc
     1141 atgttatggg attaatttta gccctaactt atttacaaaa tgtaaaaggt accaatgcat
     1201 ggggtgttta cagaatgtaa gccaaaaaat gcaccaggtc taaggcagaa atgtttagtt
     1261 attacccaga gccttggcta aatcatttag ctggctcatc cataatgctt ttgaactttc
     1321 tacatcctct ttataagcgt aatctgtaac agtaagaata gtttctttgg taatatccgt
     1381 aactctgatg cgatattcaa aataagcacg gtcatcatca tcatcttccc aacgcagctt
     1441 tacaaattct tcctcttcat aagccagcac ttcggcattt tgttcttctc cattccattc
     1501 aaaggtaaaa acctctccgt ttggatctaa atcgacataa tcagcaaacc actgtaccaa
     1561 accggagggc gtagtgagga agctgtataa aatagcaggc gacgatcgaa aggtgcgctc
     1621 aacggtaaat ggtactttct ttgccataat cttacattaa tttgcgcaat ttaaacaaaa
     1681 aaaacagttc gccaaatttt tatcattttt tttcaaaaaa actcaaattc tatgtttaag
     1741 gctaaaaata ttatgctaac tttatcaata tcagctgact gtcttttagt tggaactagt
     1801 attttgtatt tcaaataaaa aaacttattt tcgcacacta aaatttttga cgacaaaaaa
     1861 attggatgat ttttaaacat tattaagatg ctatcaaaag aaaaaaaact agaaattttt
     1921 aaagaattcg gcggagaagc tactaatacc ggctctgttg aatcgcaagt ggccttgttg
     1981 acaaaccgta tcaatcacat atccggtcac ttaaaaacaa acagaaaaga cttttctact
     2041 acacgctcac tttacaaatt agttggtcag cgtaagaaac tcttaaaata tcttgcaaac
     2101 aaagatatcg taagataccg tgctttatta gataaactcg gattgagaaa ataatatact
     2161 taacagaaag cgaggtttga gcctcgcttt tttcattttt tacttcactt aatatttaat
     2221 tttatgcaat tcaacataca tactcagatt gttgattatg gtgacggcag aaccgtaacc
     2281 gtagaaaccg gaaaactagc tcgtcaggca gacggagctg ttaccgtgcg ttgtggcaac
     2341 tgtatcattt tagctacagc ttgtgcaagc gaagaaccta aagttggtat tgactttttt
     2401 ccacttaccg tagaatatca ggaaaaattt gcctctgccg gcagaatacc gggtagcttt
     2461 atgcgtcgcg aaggcaaatt atccgatcac gaagtgctga tttcaagact agttgaccga
     2521 gccattcgtc ctatgtttgc cgacaatttt aaaaatgaca cgcaagtatt gatttacctg
     2581 atatccaatg atgataatgt agacccggat acattagtag gcttagcagc ttcatctgcg
     2641 ttatccgttt cttctattcc gtttgaaggc cccatctccg aagttcgtgt agtaaaagca
     2701 aatggtcaat ttattgtcaa tccttatcgc aatgtgaaag acagtcttga actggacatc
     2761 atattagcag gaactgcaga aaatatttta atggtggaag gcgaagcaaa agagtgttca
     2821 gaagctgatt tagtagaagc catcaaattg ggacatgaaa ttattaaaaa acaatgtaca
     2881 gcacagttgg agctgataca aaaggccggc aaaaccaaaa tggaagttac agcaccggag
     2941 gaagatgagg aactgcaagc taaaattact gcttttgcca aagacagaat cttgacaatt
     3001 gccaaatcag ccttaggcaa acatgaaaga aaaacaggat taaaacaaat taaagctgat
     3061 tttatagacg ctatacctga agaagaactg gaaacattca acaaagattt attttctaag
     3121 tattatgcca agttggaaaa agaaaccata cgcgaaatga tactgaatga aaagatgcgt
     3181 ctggatggca gaaaaacaga tgaaattcgt ccgttattta tggaagttga cgtattgcct
     3241 tcgccacacg gttcggcatt atttacccgt ggcgaaacac aatctttaac aacagttacc
     3301 ttaggctcaa aacaagatga acagcttatt gacaaggcag aagatttgag ctataataaa
     3361 tttttactgc attacaactt ccctcctttt tcaacaggtg aagtaaaacc gatgcgtggt
     3421 acatccagaa gagaagtagg tcatggaaat ttggcaatgc gctcgttaaa acaaatgttg
     3481 cctgccgaag agagcaatcc ttacacatta cgtgttgttt ctgatatctt agaatcgaac
     3541 ggttcatctt ctatggcaac ggtatgtgca ggtacattag ccttgatgga tgcaggtgta
     3601 caaataaaaa aacacgtttc aggtattgct atgggtatga tttcgaaaga cgaaaaatat
     3661 gccattttat ctgatatact gggagacgaa gatcacttag gggatatgga ctttaaagta
     3721 acgggtacaa gagacggaat atgcggagta caaatggact taaaaattga cggactttca
     3781 tatgtagtac ttgctgaagc attagagcaa gcccgcaaag gaagattaca tatcataaat
     3841 gcaatggaaa tctgtattcc tgccgcaaga cctgacataa aaccacacgc tccaagagta
     3901 gaaaatataa ttattccgaa agaatttatc ggagcagtaa tcggtcctgg cggaaaagtg
     3961 atacaggaaa tgcaggaagt gacgaatacc acaataacca tagaagaaaa taaagaaaaa
     4021 ggcataggta acgtaattat ttttgccaat aataaagag
//
LOCUS       DAOQII010000233         4033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13598_length_4033_cov_5.949472, whole genome shotgun sequence.
ACCESSION   DAOQII010000233 DAOQII010000000
VERSION     DAOQII010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13598_length_4033_cov_5.949472"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLF48_09975"
     CDS             complement(<1..641)
                     /locus_tag="PLF48_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49696.1"
                     /translation="MRTNRTYLLLLLLLQTHLFYSSAMAQTNYDNLWKSIEKLENDGK
                     TKDALTSAASILDKAKTEHNTAQIVKASLYTYKYKMILEENSELSIVNDLKQNIQSTS
                     GAEKAILQSILAELYWQYYNSNSWKFSNRTETQEKQNDDFRTWDLKTLFKEINIYYMA
                     SLENKTLLQQTSLESIKPILEEQKNSKIYRPTVYDLLAHRALSYFNDDKSDIAS"
     gene            773..1684
                     /locus_tag="PLF48_09980"
     CDS             773..1684
                     /locus_tag="PLF48_09980"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HNF49697.1"
                     /translation="MSVFKANGKLLLTSEYFILDGAVGLCLPAKLGQTLKVTLSENIC
                     SSWQSFNADGSLWFACQFDVDLNCMESTDAAIAERLMQIFKAAQNLNPEIKPESQHFE
                     THLQFPNNWGLGSSSTLISLVAQYFSCNPYELLAATFGGSGYDIACATANSPILFNRN
                     NENQINIQTADFFPIFYENLYFIHLNRKQNSREGIAHYKQLPLSDRLAIIPALNEQSN
                     RILHAKDLNHFISLLEKHESLIAENLDLPKVKTLHFSDFPGAVKSLGAWGGDFVLAAS
                     HLTQEELTAYFNTKGYFTIISYNSLIL"
     gene            1771..3330
                     /locus_tag="PLF48_09985"
     CDS             1771..3330
                     /locus_tag="PLF48_09985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF49698.1"
                     /translation="MKEVVTNEMKISGIKERSESAKKLYPYQENYIRHIFEEIQQLDD
                     HSQKENIIFQLPTGGGKTVIFSEIARKYIEQYHRKILILTHRIELLKQTSQAIEDAGI
                     HCKLITSEVEDIPDQEQYMCFLAMVETLNNRLKDDDGYLDDISLVIVDEAHYNSFRKI
                     FHYFRNAILLGFTATPLSSNITLPLRQNYKKLIIGDSIKELIKDGYLSDATTYTYDVH
                     LGGLKVGIDGDYTVSSLDRIYMGFEMHEKLLYAYREKAKDKKTLIFNSSVATSKSVEK
                     FFTDQDVPIKHLDSTSNKQERKDVLQWLKETPNAIVTSVGILTTGFDEPTVECIILNR
                     ATRSLTLYHQMIGRGSRRLPHKNHFTIIDLGNNAKRLGLWQDFIDWRDVFVNPEKFLQ
                     HLNEREIQMEKGLLYELPNTVRELFPNTQHFEFDMEREYYALYNRGKKTLDSLDISLE
                     NHYKRIFENTTNYLDALPLINALQDEIQYRLNVYISCLSKATRNYFNYLLENYNEKLH
                     QRLKSTLPFSE"
     gene            complement(3334..>4033)
                     /locus_tag="PLF48_09990"
     CDS             complement(3334..>4033)
                     /locus_tag="PLF48_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49699.1"
                     /translation="NEKSTIFFTLIFLTRTQVYKIIQQKFTNVLSLQENTMKTVLKVI
                     GFLLLFILIIPSFRQKALNLFFPPKYGKGISYKELEKASEKYKKEHGLPIKTFTSPNM
                     KVNIFSIEEWTPKSSENLLQAPYAHYLVVKTSVENTSDSEAQTGMFNTTFFLEDDAGN
                     LSHAKLENFVGYYSENNISPQEEVINAYYTGKTPPHTALQPKLFFFPMHGNSKVVKVH
                     FDDWISKDQMKFDL"
BASE COUNT         1287 a          680 c          764 g         1302 t
ORIGIN      
        1 gatgcaatgt cacttttatc atcattgaaa tacgataaag ctcgatgtgc taagaggtca
       61 tatactgtag gtcgatagat tttggagttt ttctgttctt ctaaaatcgg cttgatagac
      121 tccaaagatg tttgttgtaa aagcgtttta ttttctaagg aagccatgta atagatgttg
      181 atttccttga acaaagtttt taaatcccaa gtacggaaat cgtcattttg tttttcttgt
      241 gtttcggtgc ggttggagaa tttccaggag ttgctgttgt aatattgcca atatagttcc
      301 gctaatatag attgtaaaat tgctttttca gcaccggaag tgctttgtat gttttgtttt
      361 aaatcattga caatggacaa ttcggaattt tcttccaata tcatcttata cttgtaggta
      421 tatagtgatg ctttgacaat ttgagccgta ttgtgttcgg ttttcgcttt gtctaaaatt
      481 gaagctgctg acgtcagggc gtcttttgtt tttccgtcgt tttctaattt ctcaatagat
      541 ttccagagat tgtcgtaatt cgtttgtgcc atagcagaag aataaaataa atgggtttgc
      601 aataacagga gcaaaagcaa ataagttcga ttggttctca tatcgttaag attaataatg
      661 ttaaattaca ctttctaaca acaaaaagtg tactaaagat gagaattaag gaattttaag
      721 gtttaatttt cattttgaat taacttgata aagtgtatgt ttgtcattct gcatgtcagt
      781 atttaaagcc aacgggaagc tattgctcac atccgaatat tttattttgg atggcgctgt
      841 cggactatgt ttgcctgcca aactaggtca aacccttaaa gttactctat cggaaaatat
      901 ctgcagttca tggcagtcgt ttaatgcaga tggcagtcta tggtttgcct gtcaatttga
      961 tgtggacttg aattgcatgg aaagtaccga tgccgcaata gcagaaaggc ttatgcagat
     1021 ttttaaggcg gcacaaaatc tgaatccgga gataaaacca gaaagccaac atttcgaaac
     1081 tcatctgcaa tttcccaaca actggggttt gggcagcagt tcaactttaa tcagtttagt
     1141 ggctcaatat ttttcgtgca atccctatga gctgctcgca gccactttcg gaggttcggg
     1201 ctatgatatt gcatgtgcaa cggctaatag cccgatatta tttaatagaa ataatgaaaa
     1261 tcaaattaac atacaaacag cagatttttt tccaatattt tatgaaaacc tgtattttat
     1321 tcatctcaac agaaaacaaa attcgagaga aggaatagca cattacaaac aattgccttt
     1381 atcagataga ttggcaatta ttcctgcttt aaatgaacag tcgaaccgga ttttacatgc
     1441 aaaagatttg aaccatttta tatcgctttt ggaaaaacat gaaagcctga ttgcagaaaa
     1501 tttagatttg cccaaagtaa aaacattaca tttttcagac tttcccggtg ctgtaaaatc
     1561 attaggtgcc tggggtggcg atttcgtatt ggctgcaagt catcttactc aggaagaatt
     1621 gacagcctat tttaatacaa aaggctattt tacaattatt tcatacaata gccttatttt
     1681 ataatctcaa tttaagttct acaaggaata atatgcttat attacgcagt tcgattttga
     1741 acagctaaaa agtattggta tagtatttgt atgaaagaag tagtaactaa tgaaatgaag
     1801 atttctggaa taaaagagcg ttcggaatca gcgaaaaaat tatatcccta ccaggaaaat
     1861 tatattcgtc atatttttga agaaatacag cagttggatg atcattcgca aaaagaaaat
     1921 atcatctttc agttgcctac aggtggcggc aaaacagtca ttttttctga aattgcccga
     1981 aaatatattg aacaatatca ccgcaaaatt ctaattctta ctcaccgtat tgagctgctc
     2041 aaacaaactt ctcaagcgat tgaagatgcc ggcattcact gcaagctgat tacctcggaa
     2101 gtggaagata tccctgatca ggaacaatat atgtgctttt tggcaatggt ggaaacgctg
     2161 aacaacaggc ttaaagatga cgacggatac ttagatgata tcagtttggt tatagtggat
     2221 gaggcacatt acaattcttt ccgcaagatt tttcattatt tcagaaatgc cattttactc
     2281 ggtttcaccg ctacaccact gagttctaat atcaccttac cgcttcgtca aaattataaa
     2341 aaactgatta tcggcgattc gattaaagag ttgataaaag acggctatct cagcgatgcc
     2401 actacttata catatgatgt gcatctaggt gggctgaaag tgggtatcga cggcgattat
     2461 accgtcagct cgctcgaccg tatttatatg ggttttgaga tgcacgagaa attattgtat
     2521 gcttaccggg aaaaagcaaa ggataaaaaa acacttatct tcaacagttc tgttgctacc
     2581 tctaaatccg tagagaaatt tttcactgat caggatgtgc ctatcaaaca cctcgacagt
     2641 acgagcaaca aacaagagcg caaggatgta ttgcaatggc tgaaagaaac gcccaatgca
     2701 attgttacct ccgttggcat tttaactacc ggatttgatg agccgacagt agaatgtatc
     2761 attttaaaca gagctacccg ctctttgact ttatatcatc agatgatagg gcgaggcagc
     2821 agacgcctgc cgcataaaaa tcattttacc ataatagact tgggaaataa cgccaagcgt
     2881 ttgggtcttt ggcaggattt tattgattgg agagatgtat ttgtcaatcc cgaaaagttt
     2941 ttgcagcacc tgaatgagcg tgaaatacaa atggagaaag gattactgta tgaattgccg
     3001 aatacagtga gagagctttt tcccaataca cagcattttg agtttgatat ggagcgggaa
     3061 tattatgcct tatataaccg tggcaaaaag accctcgatt cgctggatat ttctcttgag
     3121 aatcactaca aacgcatttt tgagaatacg acaaattatt tggatgcatt acccttgatt
     3181 aatgcattac aagatgaaat ccaatatcgc ttaaatgtgt atatcagttg cctgagcaaa
     3241 gctacgcgca attattttaa ttatctttta gaaaactaca acgaaaaatt gcatcaacgg
     3301 ttaaaatcta ccttaccttt tagtgagtaa ggtttacaaa tcaaatttca tctgatcttt
     3361 tgaaatccag tcgtcaaaat gaactttaac gacttttgaa tttccatgca tcggaaagaa
     3421 aaataattta ggctgaagtg ctgtatgcgg aggcgttttt cctgtatagt aagcattgat
     3481 aacttcttct tgaggcgaga tattattttc ggaataatag cccacaaaat tttcgagttt
     3541 cgcatgggat aaatttccgg catcatcttc cagaaaaaaa gtggtattaa acatgccggt
     3601 ttgtgcctca ctatcagatg tattttctac ggatgttttt accacaaggt aatgcgcata
     3661 aggagcttgt aataaatttt cagatgactt tggtgtccat tcctctatag aaaaaatatt
     3721 taccttcata ttgggtgaag taaatgtttt aattggcaaa ccatgctcct ttttgtactt
     3781 ttcagaagct ttttcaagct ctttataaga tattcctttg ccatatttag gagggaaaaa
     3841 caaattcaat gctttttgtc gaaaggatgg aattataaga ataaataaaa gtaaaaaacc
     3901 aattactttt aaaacagttt tcatagtatt ttcttgtaaa gataaaacat tagtgaactt
     3961 ctgctgaatt atcttataaa cctgagttcg ggttaagaaa attaaggtga aaaaaatagt
     4021 tgatttctca ttt
//
LOCUS       DAOQII010000234         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13656_length_4022_cov_5.422486, whole genome shotgun sequence.
ACCESSION   DAOQII010000234 DAOQII010000000
VERSION     DAOQII010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13656_length_4022_cov_5.422486"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..738)
                     /locus_tag="PLF48_09995"
     CDS             complement(40..738)
                     /locus_tag="PLF48_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNF49700.1"
                     /translation="MSKVFKNAEEALFDIQDNMTIILGGFGLCGIPENAIAALVKKGT
                     KGLTCISNNAGVDDFGLGLLLQGHQIKKMISSYVGENAEFERQLLNGELEVELIPQGT
                     LAERCRATAAGIPAFYTPAGYGTEVAEGKETREFNGKMYIMERSFDADFAIIKAWKGD
                     ANGNLIFRETTRNFNMPMAGAGKVTIAEVEELVGIGELNPDQIHVPGVFIKRIFQGEQ
                     YEKRIEQRTVRQKV"
     gene            complement(865..3090)
                     /locus_tag="PLF48_10000"
     CDS             complement(865..3090)
                     /locus_tag="PLF48_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49701.1"
                     /translation="MKNILITLLLTFVLFQTGFAQKKMKFEEAKELYSRLAYSEAITL
                     LSDYLKGADNIEAKQLLAQCYVHTNNYEQAEKTLAEIVKDTSNTNNMYILCYAKTLQT
                     LGRYNEAVGWYAKYLSQNPNSSFAKNQEFACKNKITYFGDSLLFLSEHLPFNTDGLDF
                     GACIRKDVFIYTSTGGKTNSKNKNIDLYTDEPFMDIYEVKFDDTALNFSTAQIFNAGI
                     NTKYHEGPLAFSPLGKEVYFTRNDYNPYKSVKKVGCDNDKIINLKIFKAYLENGKWVR
                     DEELSFNSSEYSCGHPTVDFKKQIMYFTSDMPGGYGGTDLWYSKFNGTRWMQPINAGP
                     KINTDGNEMFPYLATDGILYFSSNGQAGLGGLDIFAATLKNNEVVEVKNLNSPINSSY
                     DDFAFLLRDNGRVGFYSSNRPGGNGNDDIYLIYKNVFKLEGKVINSRTNLPLANAEVK
                     MSSNNQQLHTFKTDKNGNFITEVRGLMTYDFWADASNYIPSTSQISIPATKQDQTYKI
                     TIPLQPIVAEITYFDKLTKLPIATAHSDVTTCQEVKTSISSADNGKAYMDVLENCKYK
                     LLSNAKGYFPEQSAFEIGELQDTFRTVVELKPINYDIMTLNNIYYDFDKCYIRPDAVP
                     DLSMLLSFIKTNPDAQVELSSHTDARGDDDYNMKLSQCRADAAVDWLINHGVAKAQIT
                     AVGYGESKLLNMCANDISCTEEEHQKNRRTEFRVTNAGVITSSQKKDNIYTDRCKNCK
                     F"
     gene            complement(3111..>4022)
                     /locus_tag="PLF48_10005"
     CDS             complement(3111..>4022)
                     /locus_tag="PLF48_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF49702.1"
                     /translation="QEPMNTMFAYNKLQTNPAYTGGKDLLSMRAVYRTQWVKLPGAPR
                     TINFNIHSPVKKERIAVGLSVLNDIAGSVNQTNLTPSFAYRIPFKNDTKLSFGISAGI
                     KFYKINVTTLTATDVNDPLLGTNYSGVAPTMGFGLYYYGRKFYVGLSSPNILPMDLIR
                     KNKELAQMTTDRFQQVGHMYLSGGYTFEIGKKKNFWIQPQLLFKYLMTSKYKIPFEID
                     ANLTFTLYKIANIGLTYRTGFANIYDNRESIDIMTMFNLPKGFSVSYSYDITLANSKT
                     FHNNSHEILLGYDVDIRKKGVRTPRYF"
BASE COUNT         1191 a          797 c          659 g         1375 t
ORIGIN      
        1 ttttctttct ccctactact tattacgtat tactttctct tatacctttt gtctcactgt
       61 tctttgttcg attcttttct catattgctc accttggaag atgcgtttaa taaaaacacc
      121 cggcacatgt atctgatccg gattaagctc gccaatgcct accagttctt ctacttcggc
      181 aatggttact ttgccagctc cggccatggg catattaaaa ttgcgcgtag tttctctgaa
      241 aatcagattg ccgttggcgt ctcctttcca tgctttgatg atcgcaaaat cagcatcaaa
      301 actgcgctcc atgatataca ttttgccgtt aaactctctg gtttctttgc cttctgctac
      361 ttcggtgcca tagcctgccg gtgtgtaaaa agcgggaatg ccggcagccg tagctctgca
      421 cctttcggct aaagtacctt gcggtatcaa ctctacctcc aattcgccat ttaataactg
      481 acgttcaaat tctgcatttt ctcctacata ggatgatatc atttttttga tctgatgtcc
      541 ttgcaatagg agtcccaagc caaaatcatc cacacctgca ttattggaaa tacacgtcaa
      601 gcctttcgtt ccttttttta caagcgccgc tatagcattt tcaggaatgc cgcacaaacc
      661 aaatcctcct aatatgatgg tcatattatc ttgtatatca aacaaggctt cttctgcatt
      721 tttaaatact ttactcatgg attttataat aaagtttaaa gctacaaaaa aatgaagaat
      781 attaaagtga aatacttttt gaatgaataa aaaaaacgcg cctttacgac gcgtcccaag
      841 aaaataatta ctatatgaaa gtagttaaaa cttacaattt ttacatctgt ctgtataaat
      901 attatctttc ttctgagaag aagtaatcac accggcatta gtaacacgaa actccgttct
      961 tctgtttttt tgatgttctt cctctgtaca gctaatatca ttggcacaca tattcagcaa
     1021 ttttgattcg ccataaccta ctgcagtaat ctgtgctttt gctacaccat gattaatcaa
     1081 ccaatctact gctgcgtctg cgcggcattg tgacaatttc atattataat catcatcgcc
     1141 tcgtgcatct gtatgcgaac tcaattcaac ttgcgcatca ggattggttt tgataaaact
     1201 caataacatc gataggtcag gaacggcatc aggacgtata taacatttat caaaatcata
     1261 gtagatatta ttcaatgtca tgatatcgta gttaataggt ttaagttcaa caaccgttct
     1321 aaaggtatct tgcagttcgc caatctcaaa tgctgattgt tcgggaaaat accctttggc
     1381 attcgacaat aatttgtatt tgcaattttc taaaacatcc atataggctt taccattatc
     1441 agctgaagaa atagaagttt tcacttcctg acacgtagta acatcactat gggcagttgc
     1501 aatgggcagt ttagttaatt tatcaaaata ggtaatttca gccacaatgg gttgaagtgg
     1561 aatagtaatt ttataggttt ggtcttgttt ggttgcagga attgaaatct gactagtact
     1621 cggaatataa ttgcttgcat cagcccaaaa atcatacgtc atcaggcctc ttacttctgt
     1681 tataaaattt ccgtttttat ctgttttaaa agtatgaagt tgctgattat tgctgctcat
     1741 tttcacttct gcatttgcaa gtggtaaatt agttcgcgaa ttaattactt taccttccag
     1801 tttaaaaaca tttttataaa tcaggtagat atcatcgttg ccattaccac cgggtctgtt
     1861 actcgaataa aatccaactc tgccgttatc acgcaataaa aaagcaaaat catcataaga
     1921 agaattgatg ggagaattca ggttttttac ttcaaccact tcattatttt tcaaggtggc
     1981 agcaaatata tccagtccgc ctaaaccggc ttgtccattg ctcgaaaaat ataaaatacc
     2041 atcggtagcc aaataaggga acatttcatt gccgtccgtg ttaatcttgg ggcctgcatt
     2101 tattggctgc atccaacggg tgccattaaa tttggaatac cataaatctg ttccaccata
     2161 accacccggc atatcggagg tgaagtacat aatttgcttt ttaaaatcta ctgttggatg
     2221 cccgcaacta tattcactgc tgttaaaaga cagctcttca tctctcaccc attttccgtt
     2281 ttccagataa gctttaaaaa ttttcaggtt aatgatttta tcattgtcac aacccacttt
     2341 cttaacggat ttatacggat tgtaatcatt acgggtaaaa tacacttctt ttcctaatgg
     2401 actaaatgcc aatggtcctt catggtactt cgtattaata cctgcattaa atatttgcgc
     2461 tgtagaaaaa ttcaacgcag tgtcatcaaa tttaacttca taaatatcca tgaaaggttc
     2521 gtcagtatac aagtcgatat ttttgttctt actatttgtt tttcctccgg tagaagtata
     2581 gataaataca tcctttcgga tacatgcacc aaaatcaagg ccatcagtat taaacggaag
     2641 atgttcagat aaaaaaagca gtgaatcgcc aaagtaggtg attttatttt tacaggcaaa
     2701 ttcttgattt tttgcaaatg aactattcgg attttgagat aagtatttag cataccaacc
     2761 tacggcttca ttatatcgtc ctaaagtctg tagcgtttta gcatagcaca gtatgtacat
     2821 gttatttgta ttacttgtgt cctttacaat ttcagcaagt gttttctccg cttgttcata
     2881 gttatttgtg tgcacatagc attgtgcaag caattgtttt gcttcaatat tatctgcacc
     2941 tttaagataa tcacttaaaa gagtaatagc ctcactgtac gccagtctgg aataaagttc
     3001 tttggcctct tcaaatttca ttttcttttg tgcaaatccg gtttggaaga gtacaaaagt
     3061 cagcaacagt gtaatcaata tatttttcat agttttttaa atttagcagt ttaaaaatat
     3121 ctcggtgttc ttacgccttt ttttctaata tcaacatcgt atcctagtaa aatctcatgt
     3181 gaattattat ggaaagtttt actgtttgcc agtgtaatat catatgaata cgatacacta
     3241 aatcccttgg gcaaattaaa catggtcata atatcaatac tttccctgtt atcataaata
     3301 ttggcaaaac cggttctata tgttaagccg atattggcaa ttttatataa ggtgaatgtt
     3361 agattggcat ctatttcaaa aggaatcttg tattttgaag tcatcaaata cttgaacaat
     3421 aactgtggtt gtatccagaa attctttttc ttgcctatct caaaagtata tccaccagat
     3481 aaatacatgt gtcctacttg ctgaaaccgg tcagtagtca tttgcgccag ttccttattt
     3541 tttcttatca aatccatggg caaaatatta ggagaagaca acccgacata aaattttctt
     3601 ccataataat ataagccgaa tcccatcgtt ggagctactc ctgaataatt agttcccaga
     3661 agcgggtcgt taacatctgt tgcagttagc gtagtaacat ttattttata aaatttgata
     3721 ccagcactaa tgccaaaact aagtttagta tcatttttaa aaggtatgcg atacgcgaaa
     3781 ctgggtgtca ggttagtttg atttacagaa ccagcgatat cattcaatac acttaatcct
     3841 acagcaattc gttctttttt aacaggagag tgtatattga aattaatagt acgaggtgcg
     3901 cctggtagct ttacccattg cgtcctgtaa actgcccgca tgctcagcag atctttgcca
     3961 ccggtatagg cagggtttgt ttgcaattta ttataagcaa acatagtatt catcggttcc
     4021 tg
//
LOCUS       DAOQII010000235         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13657_length_4022_cov_4.853290, whole genome shotgun sequence.
ACCESSION   DAOQII010000235 DAOQII010000000
VERSION     DAOQII010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13657_length_4022_cov_4.853290"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..395)
                     /locus_tag="PLF48_10010"
     CDS             complement(6..395)
                     /locus_tag="PLF48_10010"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNF49703.1"
                     /translation="MTFEERVKLSETHIFKAVFPNTTNHMDTLFGGTAMQLMDEVAFI
                     TATRFARKKMVTVSTDKIDFNVAIPSGTIIELIGKVTKMGNTSLHVSVEIFMENMFND
                     QRILAITGEFIFVALDENKKPVKIILE"
     gene            929..2065
                     /locus_tag="PLF48_10015"
     CDS             929..2065
                     /locus_tag="PLF48_10015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF49704.1"
                     /translation="MKTKFDNINSIRLLLALVVLCLHFEAYKKHNGLKSILNDSSNLF
                     VIGEIAVYFFFTISGFLIGYNLISEKAQTNTVSLKRFYTNRALRIWPLYYLAIVLYWF
                     VLPHTVFGNILDQFPAHIYANSIIMQNKLTEHTALLLYLFFLPQLASLNAQFSGSSMY
                     PAPHLWSIGVEEVFYLLIPLFILFIRNILKKLTQVTIFYFAFPILLIIIYKLHNSKII
                     FALLFAIFMHPFCCMLLGVISSYLYYYHSDKIVRYCNKKSAAVCCMLILIMLYKSLYF
                     YWFVPAIYCLLFSVVLLYLVQHKINILSLSFIDYLGKISYGIYIYHNISIAIIIYLIK
                     YAISPKLKFMLSYPIVFVILLTVLMSVISYEFIEKRFLQLKRKG"
     gene            2065..2922
                     /locus_tag="PLF48_10020"
     CDS             2065..2922
                     /locus_tag="PLF48_10020"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF49705.1"
                     /translation="MKPTPFISRLLPFLMILFISMQLYIAQNPYYTTSNLPVLLSIGF
                     ILMVTLFEIIFPYRNDWHFWNDRQSFNDIILYLVNTIVIIKCVPSLTILKNKLHLANV
                     TIWPEELPLLIQLLLAFIIFDFFYYWFHRLSHTISYLWIFHAVHHSAEKIHFLKGNRS
                     NLFFDALPYFVLTVFPLAFLNPPNQIFIMMGIILRDFSFMIHSNISLQFPSWWEKLFV
                     SPKLHQMHHDKFIDNGMGYNFSTLTLIWDKIFGTFKPFDNLKTISTGIENDPVSKNVF
                     WQIFFPFKK"
     gene            3040..3639
                     /locus_tag="PLF48_10025"
     CDS             3040..3639
                     /locus_tag="PLF48_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF49706.1"
                     /translation="MLIRHTSMTTMLSLNMAKENEVSIRNTISQYGKRLFGYIRSKVK
                     TNEDAEDILQDVWYQLSNFGNLSEIENLSAWLYRVAGNKVTDAYRKKSNDLLDDFVIE
                     DEDGSFDFKEILLLDDSKNPDMVFFKELFWDELIRALDELPEKQREVFVLNEIQDMTL
                     QEIADQSGENLKTIISRKGYAVKHLRERLHYLYLELKNN"
     gene            3749..3967
                     /locus_tag="PLF48_10030"
     CDS             3749..3967
                     /locus_tag="PLF48_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMG-box domain-containing protein"
                     /protein_id="HNF49707.1"
                     /translation="MPLLFHLTVINFWQALGLLVLSKILFGGFRFCGKHRHRPPFANG
                     GMKEKWANMTDEEKSAFKQQWKARCGKE"
BASE COUNT         1332 a          649 c          653 g         1388 t
ORIGIN      
        1 tataattatt ctaaaataat tttaaccggt tttttgtttt catccaaagc cacaaaaata
       61 aattcgcctg tgatagccaa aatacgttgg tcattaaaca tgttttccat aaaaatttcg
      121 acactgacat gcaaactggt atttcccatt ttagttacct tgccaatgag ttcaataatt
      181 gtgccactgg gaatggcaac attaaagtca attttgtcgg tagatacagt caccatcttt
      241 ttgcgggcaa atcgggtagc ggtaataaat gccacttcat ccataagctg catagcagta
      301 ccgccaaaca aagtatccat atggttggtc gtattgggga aaacagcctt aaaaatgtgc
      361 gtttcagaga gttttactcg ttcttcaaaa gtcatggagt gaagatacgg aaataaaatt
      421 ttacaaagat gaatctaatg aatgctcagg tattgacaga attagagctg caaaatttga
      481 aaagcgttta tccttctatt agccttaaaa tactaagctg ttagctgtgc tcgactttga
      541 ctatattctg tacaaataac cgtataaatt actgacaatc tgtattatgg attctataaa
      601 cttactagtc atttcaattt tgtcaaaaat atgtattaga taaacctaaa tttaatactt
      661 aattttattg gacagaatgc tataaaaagg catcactcct aatgaaatca atcaagatac
      721 ataaaaagac atcaacggcc gttggtatat tctgttttat attagtattt ttagttcaag
      781 cttattttag catacaattt tgcacgcctc caaaaacaga cagtcatttg gcagaatgcg
      841 agattcaaat gaagcaaaaa acaaggtatt ttaatctggt tggatttgca gtaaacattt
      901 tccaaaaaac aatgagcaaa cagatgttat gaaaacaaaa tttgataaca tcaacagcat
      961 tagattatta ctggcactgg tggtattatg cttacacttc gaagcataca aaaaacataa
     1021 tgggctaaaa agcatattga atgatagcag caatttattt gtgattggtg aaatcgctgt
     1081 atatttcttt tttactatca gtggttttct aattggctat aatctgattt ctgaaaaagc
     1141 gcaaacgaat acagtttcat taaaaagatt ttacacaaac agggcattgc gcatttggcc
     1201 cttatattat ttagccatcg tgttatattg gtttgtcctg ccacatactg tattcggcaa
     1261 tatattagat cagtttccgg cacatattta tgccaatagt attatcatgc aaaacaagct
     1321 tactgaacat accgccttat tattatatct tttcttttta ccacaactag cttctttaaa
     1381 tgcacaattt tcaggctcaa gtatgtatcc tgcgcctcac ctttggagta ttggcgttga
     1441 agaagtattt tatttactca tacccctatt tatcttgttt atccggaaca ttttaaagaa
     1501 actgacacaa gtaaccattt tctattttgc attcccgatt ctgttgataa ttatatacaa
     1561 actacacaac agcaaaataa tttttgcact tcttttcgca atattcatgc accctttctg
     1621 ctgtatgttg ttaggagtta ttagtagtta tctgtactat tatcattcgg ataaaatagt
     1681 tcgatattgc aataaaaaat ctgctgcagt ttgctgtatg ttgatcttga taatgttata
     1741 taaaagcctc tatttttatt ggtttgtccc tgcaatttac tgtttgctct ttagtgtagt
     1801 tttattatat ctggtacagc ataaaatcaa catcttgagc ctgagtttta ttgattattt
     1861 aggcaaaatc tcttatggca tctatatcta tcataacatt tcaatagcaa taatcatata
     1921 tttaatcaaa tatgctattt cccctaaact aaaatttatg ctttcttatc ctatagtatt
     1981 tgtcattttg cttactgtat tgatgtccgt tatttcctac gaattcattg aaaaaagatt
     2041 tttgcaactt aaacgaaaag gataatgaag cctactccgt tcatcagccg cttgttgcca
     2101 tttttgatga ttttatttat ctcaatgcag ctctatatag cacaaaaccc atattatacc
     2161 acatcaaatt tgccggtatt attatcgatt ggatttattt tgatggtaac gctttttgag
     2221 attatttttc cttatcgaaa tgactggcat ttttggaatg accgccaaag ctttaatgat
     2281 attattttat atctggtaaa tacaatagtc attataaagt gtgtaccaag tttaactatc
     2341 ttaaaaaaca aattacatct cgccaacgta accatctggc cggaagaact accgctttta
     2401 atacaacttt tattggcatt tatcatcttt gatttttttt actattggtt tcatcgttta
     2461 tctcatacta tcagctactt gtggatattt catgcggtgc atcacagtgc cgagaaaatt
     2521 cattttttaa aaggcaatcg aagcaattta ttttttgatg ccttacctta ctttgtttta
     2581 actgtttttc cattggcttt tttaaatcct ccaaatcaaa tttttataat gatgggcatt
     2641 atattgagag attttagttt tatgattcat tccaatatta gcttacagtt tccatcctgg
     2701 tgggaaaaat tatttgtatc tccaaaatta catcaaatgc atcatgacaa atttattgat
     2761 aatggaatgg gctataactt tagtacgcta accttaatct gggataaaat ttttggaact
     2821 tttaagcctt ttgacaactt aaaaaccatt tctacaggga tagaaaatga ccctgtaagc
     2881 aaaaatgtat tttggcaaat cttctttcca tttaaaaaat aaatagtaca caccgcatta
     2941 aataaataaa ttctactagc cagtaattca tcagatgtaa ttttctcttt tcatttagca
     3001 gaaatacaag ataaaattga tagatttaaa gtaatttcct tgcttatacg tcatacaagt
     3061 atgacaacta tgctgagttt gaatatggca aaagaaaatg aagtttcgat acgcaatact
     3121 atcagtcaat atggcaaaag actgtttggc tatatccgtt caaaagtaaa aacgaatgaa
     3181 gatgctgaag atattttgca agatgtttgg tatcagttga gtaacttcgg aaatttgagt
     3241 gaaatagaaa acctgagtgc gtggctttat cgagtagccg gaaacaaagt gacagacgct
     3301 tatcgaaaaa aaagcaatga tttattagac gatttcgtga tagaagatga agacggaagt
     3361 tttgatttca aagaaatatt actgcttgat gacagcaaga atcctgatat ggtttttttt
     3421 aaagaattgt tttgggatga acttatcaga gctttagatg agttgccgga aaagcaaaga
     3481 gaagtatttg tgttaaacga aattcaggat atgactttac aagaaatagc tgaccaaagt
     3541 ggggagaatc ttaaaacgat aatcagcaga aaaggttatg cagtgaagca tttgcgtgaa
     3601 agattacact atttatattt agaactaaaa aacaactgac atgtttaaaa acaataaaaa
     3661 caacccccga atgctatttt tcctgcttat atttctgtgt gggatttcag ctttgacagc
     3721 tcttacaatg ttattatgga attggctgat gccgttatta tttcatttga ctgtcatcaa
     3781 tttttggcaa gccttagggt tactggtgtt gagtaaaatt ttattcggag gatttcgttt
     3841 ttgcggcaaa cacagacatc gtccgccttt tgcaaatgga ggcatgaaag aaaaatgggc
     3901 caatatgaca gatgaagaaa aatccgcctt taagcagcaa tggaaagcca gatgcggaaa
     3961 agaataacag tttaagattt tactttggag aagaagaaat ttctcaatca ttacaaaagt
     4021 tt
//
LOCUS       DAOQII010000236         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13774_length_3999_cov_4.645538, whole genome shotgun sequence.
ACCESSION   DAOQII010000236 DAOQII010000000
VERSION     DAOQII010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13774_length_3999_cov_4.645538"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..621
                     /locus_tag="PLF48_10035"
     CDS             154..621
                     /locus_tag="PLF48_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49708.1"
                     /translation="MAVFFYFKNITNQKKIENLMLNEFQEAISEPIISYSLFTVDNTT
                     DCRFINQASADFPHHFGILKNQFIEIDWNIINADWISIEGVGYVQSIGKMAFYPVENT
                     VYKLKAKNKEIEMEQAIYVRVFPVNINAELFEKVPEIRFKTELQIPKMVIGDW"
     gene            788..>1977
                     /locus_tag="PLF48_10040"
     CDS             788..>1977
                     /locus_tag="PLF48_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HNF49709.1"
                     /translation="MTEFKNNPSYTSPLPGGFTKFLWWCSGANADILRDSPQSEHNKY
                     QGIGATVFFTGVLAALSGAYALYSVFNNLYAAMAFGILWGLVIFNLDRFIVSTIRKED
                     NFWNEFKLALPRIVLAIILAIVIAKPLELKIFEKEIAQKIQEQQQEFAMQSQKKIQTQ
                     FNPIADSLRIEIDYYKNEIAKVAAARDTLYQAFIGEAEGTSGTHKLGKGPVFKEKKIQ
                     YDKIEQEFKSVQEKYQPLIDEREKQILAIKSQRDTAYAKSVPVISNYDGLMAKLDAEN
                     KLPQLPVFFITLLFICIETAPVMAKLLSKKGPYDEKLRNIEHEIELNTIANINQRNHD
                     LNKKLTLYTNIDKAKVEQEIKQNEDALKIIANAHLQLTQDYVDDWLEEERAKLNRNKY
                     KSNR"
     assembly_gap    1978..2077
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2303..>3999
                     /gene="pheT"
                     /locus_tag="PLF48_10045"
     CDS             2303..>3999
                     /gene="pheT"
                     /locus_tag="PLF48_10045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNF49710.1"
                     /translation="MKISYNWLKSHIDIPFSIEELKDTLTDIGLEVEAVEPFESIKGG
                     LKGLITGHVIEKEKHPDADKLSVCKVDTDTEILQIVCGAPNVAAGQKVIVATVGSTVY
                     PTNGEAFAIKKAKIRGVESFGMICAEDEIGLGTAHDGILILDKQTEVGKQVAELYNVE
                     NDYLFEIGLTPNRTDAFSHIGVARDLAACLTFRTKKAYKLSEAETVNYLENCKNLHND
                     FSVEIENKAACPYYSGIILTDIQVKESPQWLKNRLLCIGQKPINNVVDITNFILHEYG
                     QPLHAFDLNEIKGNKIIVKNLPEKTKFLALDNSTIELHQEDLMICDGDENGMCIAGVY
                     GGLESGVKNSTTAIFLECAYFDPKTIRRTSMRHTLRTESAIHFEKGIDPNIQEKALNH
                     AIHLLTALANAQPASPVYIAQSEQIRFDGKNYKAPFDVTLALSDIHKLTGAAISKKEA
                     EEIAGLLDIQVKDISTDDSLHLAVAPYRTDVYRPADVIEEILRIYGFNNVPIPTKLNA
                     SIQFSKHDFKDKLYNKTADFLVARGFFEMMNNSIVKSKQQAALKNENEENWVRLLSS"
BASE COUNT         1348 a          660 c          717 g         1174 t
ORIGIN      
        1 ttctcaaaat ttgctttttc ttaaaaattc ttaaagttta ctatttcgga actgttcgta
       61 ataatttttc gttaatgtag acgtattaaa acaaagtatc cgacattgat aaatacgcta
      121 ttcattttcg ctttaatttc cttgacggta atcttggctg tattttttta tttcaaaaat
      181 ataacgaatc aaaaaaagat agagaatttg atgctcaatg aatttcaaga agccatttca
      241 gagcctatta tttcttattc tttatttact gttgacaaca caactgattg tagatttatc
      301 aatcaagcat cagcagattt tccgcatcat tttggtatac tcaaaaatca gtttatcgaa
      361 attgactgga atattatcaa tgccgactgg atttctattg aaggtgtagg ctatgtgcaa
      421 tcgataggaa aaatggcatt ttatcctgtt gaaaatacag tttataaact aaaagcaaaa
      481 aataaagaaa tagaaatgga acaagctatt tacgtacgag tttttccggt aaatattaat
      541 gctgaactgt ttgaaaaagt accggaaata agatttaaaa cagaattgca aattccgaaa
      601 atggtgattg gtgattggta attagttatt ggtcattggg gattaattgt tggcgattgg
      661 tcattagata attgataata ctaacaacat cttgattctt ggctctcaat actcaaatct
      721 caatacttac tactcaaatc tcaatactca ctacttacta ctcactactc acatctaaaa
      781 aaacaacatg acagaattta aaaataatcc atcctacact tctcctctac ccggagggtt
      841 tactaaattt ttgtggtggt gcagtggtgc caatgccgat attttgcgtg atagtccaca
      901 atctgagcat aataaatatc agggtattgg ggctaccgta ttttttacgg gagtgctggc
      961 tgctttatcc ggtgcttatg ccctttattc tgttttcaat aatctatacg ctgctatggc
     1021 atttggcatt ttatggggac ttgttatttt caatctcgac cgttttattg tttctaccat
     1081 tcgcaaagaa gacaattttt ggaatgaatt taagttagct ttaccgcgta ttgttttagc
     1141 catcattctt gccattgtca ttgcgaagcc tttagaatta aaaatatttg aaaaagaaat
     1201 tgcgcaaaaa attcaggaac aacaacaaga atttgccatg caatcgcaga agaaaataca
     1261 aacgcaattt aatccgattg ccgacagtct gcgtatagaa atagattatt ataaaaatga
     1321 aatagctaaa gttgctgctg ctcgcgacac gttatatcag gcatttattg gcgaagcaga
     1381 aggaacaagc ggcactcata aactgggcaa gggaccggtt tttaaagaga aaaaaataca
     1441 gtacgataag atagaacagg aattcaaatc cgtgcaagaa aaatatcaac ctttgattga
     1501 tgaaagagaa aaacaaatac tcgcaattaa gtcacagcgt gatactgcct atgcaaaatc
     1561 agtgcctgtc atcagtaatt acgatggatt gatggcaaag ctggatgccg aaaataagtt
     1621 gccacagttg cccgtatttt ttatcacctt gttattcatc tgtatagaaa cagctccggt
     1681 aatggctaaa ttactttcta agaaaggacc ttatgatgaa aaactcagaa acattgaaca
     1741 tgaaatcgaa ttgaatacta ttgccaatat caatcaacgc aatcacgact taaataaaaa
     1801 actgacgctc tacaccaata ttgacaaagc aaaagtggaa caggaaatca aacaaaatga
     1861 agatgcgctg aaaatcatcg ctaatgccca tcttcagctt actcaagact atgtagatga
     1921 ctggctggaa gaagaacgag ctaaattaaa ccggaataaa tacaaaagca atagggannn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc tggaagaaga acgagctaaa
     2101 ttaaaccgga ataaatacaa aagcaatagg gagtgagtag taagtagtaa gtagtaagtt
     2161 ggcagttaaa tcacaacttt aaatattttt gtcttgattc ttaaaatctt gactctcctg
     2221 tctaattatt cttttcttaa gtttaacctt cgtatttgtc cgaacctgtc tttatttgta
     2281 ttttcatcgg ataaattagg atatgaaaat ttcgtacaac tggctaaaat cacatattga
     2341 tattccgttc tctattgaag aacttaaaga tacgctcaca gatatcggct tggaagtgga
     2401 ggcggtagag ccttttgagt ctataaaagg cggactgaaa ggactgatta ccggacatgt
     2461 catcgaaaaa gaaaaacacc ctgatgctga taaattatct gtttgcaaag ttgatacgga
     2521 tactgaaatt ttgcaaattg tgtgtggcgc acccaatgta gcagccggtc aaaaagtaat
     2581 tgtagccact gtcggcagca cagtatatcc tacaaatggc gaggcatttg ccatcaaaaa
     2641 agcaaaaatt cgcggagtag aatcttttgg aatgatatgt gccgaagacg aaatcggatt
     2701 aggcacagca catgatggca ttttaatatt agacaaacaa actgaagttg gcaagcaggt
     2761 tgctgaactt tataatgtgg aaaatgacta tttatttgaa ataggtttaa ctcctaaccg
     2821 tacggatgca ttttctcata ttggcgtagc cagagattta gcagcttgtc ttacattcag
     2881 aactaaaaaa gcatataagc tgagtgaagc agaaacagtc aattatttgg aaaattgtaa
     2941 aaatctgcat aacgatttta gtgtcgaaat agaaaataaa gcggcttgtc catattacag
     3001 cggtataata ttaacggata ttcaggtaaa agaatcgccg caatggctga aaaacagatt
     3061 gctttgtatc ggtcaaaagc ctatcaacaa tgtagtagat attaccaatt ttattttgca
     3121 tgaatatggc cagccacttc atgcttttga tttaaatgag ataaaaggaa ataaaatcat
     3181 tgtaaaaaat cttcctgaaa aaactaaatt tcttgcatta gacaacagca ccattgaatt
     3241 gcatcaagaa gatttgatga tttgcgatgg tgatgaaaat ggcatgtgta ttgctggcgt
     3301 atatggcgga ttggaatcag gtgtaaaaaa cagcacgaca gccatctttt tagaatgtgc
     3361 ttattttgat cctaaaacaa tacgtcgtac ttcgatgcgc catactttgc gtaccgaatc
     3421 tgccatacat tttgaaaaag gaattgatcc gaatattcag gaaaaagcac taaaccatgc
     3481 tattcatttg cttacagcat tggctaatgc ccaacctgca tcgcctgttt atattgcaca
     3541 atccgaacaa atccgatttg acggaaaaaa ttacaaagct ccatttgatg ttaccttagc
     3601 cttgagtgat atacataaac ttaccggagc tgctatttcc aaaaaagaag ctgaagaaat
     3661 tgccggtttg ttagacattc aagtgaaaga catcagcacc gatgatagtt tgcatttggc
     3721 tgttgcacct tacagaacag atgtctatcg ccctgctgat gtcattgaag aaattttgcg
     3781 catttacggg ttcaacaatg tgccaattcc aactaaatta aatgcctcta ttcagttttc
     3841 taagcatgat tttaaagaca aactttacaa taagactgct gattttttag ttgctcgtgg
     3901 attctttgaa atgatgaata attctatcgt gaaatcgaaa caacaagcag ctttaaagaa
     3961 tgaaaatgaa gaaaactggg tacgcttatt aagttcgat
//
LOCUS       DAOQII010000237         3983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13859_length_3983_cov_4.644603, whole genome shotgun sequence.
ACCESSION   DAOQII010000237 DAOQII010000000
VERSION     DAOQII010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13859_length_3983_cov_4.644603"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..460)
                     /locus_tag="PLF48_10050"
     CDS             complement(20..460)
                     /locus_tag="PLF48_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49711.1"
                     /translation="MKKLFILLTVFGLVSCVTKKKFQATQLEKDKIEQTLRQTTSDLA
                     DCEKKFAGKSTDYNNLMDELTKAKSTIDGLNSEVNFQKTQNIKLLDQLTNLSVISKEG
                     AESIKKSLETIDKQSRELSELNSQIRTKDSLNLVLVMNLKLIIS"
     gene            complement(471..791)
                     /gene="nuoK"
                     /locus_tag="PLF48_10055"
     CDS             complement(471..791)
                     /gene="nuoK"
                     /locus_tag="PLF48_10055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNF49712.1"
                     /translation="MNEVIIGIPIQYYIFLSVILFSLGALGVMFRRNAIIIFMCIELM
                     LNAVNLLMVAFSRYFGDSGAQVFVFFIMVVAAAEVSVGLAILMMVYRNVKSSDIDVLN
                     TLKG"
     gene            complement(832..1224)
                     /locus_tag="PLF48_10060"
     CDS             complement(832..1224)
                     /locus_tag="PLF48_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF49713.1"
                     /translation="MGTYRDLTVYKKSFTLALDIHKICSGFPKDETFGIISQIKRSSK
                     SVSASIAEGYRKRQYEAYFVSKISDADMENTETQVWLEFAKELNFIDLSDYEILLERS
                     NEVGKLLNHMINHPLKYMGSIEIMKTKK"
     gene            complement(1274..1774)
                     /locus_tag="PLF48_10065"
     CDS             complement(1274..1774)
                     /locus_tag="PLF48_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNF49714.1"
                     /translation="MLLSQYIFFFLAFLAVISALMVILDKNPVHSVLYLIVTFFAIAG
                     HYVLLNAQFLAVVHIIVYAGAIMVLFLYVIMMLNLNKEIEPHKKNLPKFIAVVTACLL
                     LVSLVSLLKGAEHSMLPALKFSNVGLVENLGNTLFNEYMLPFEVSSVLFLSAMVGAVF
                     LSKKEN"
     gene            complement(1778..2389)
                     /locus_tag="PLF48_10070"
     CDS             complement(1778..2389)
                     /locus_tag="PLF48_10070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNF49715.1"
                     /translation="MKLTNRSKLVVKKEMSLTEKLYLPAITAGMSITLKHFFKKPATI
                     RYPEVKREIAPVYRGQHVLKRDDAGAERCTACGLCAIACPAEAITMEAGERKKGEEHL
                     YREEKYATVYEINMLRCIFCGLCEEACPKEAIFLTDRIMPVSVTRNKMNYGKDLLVED
                     MNNRIDVSKRQTPQVLAFKKDKTKKHNQTWEDKQALIQENKHH"
     gene            complement(2451..3485)
                     /gene="nuoH"
                     /locus_tag="PLF48_10075"
     CDS             complement(2451..3485)
                     /gene="nuoH"
                     /locus_tag="PLF48_10075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNF49716.1"
                     /translation="MGIEVFLLYKILLVLIVFLIALGIAAYSTYGERKVAAFMQDRIG
                     PDRAGPYGVLQPLADGLKMFMKEEIIPAVSNKYLFILGPSLAMLTALMTGVVIPWASP
                     LHINGIVFPLQITDINIGILYVFGVVSFGVYGIMIGGWASNNKYSLLGALRASSQMIS
                     YEIAMGLALVALIMKTGTLSLGGIVAQQGEGFWNIIYQPLGCLIFVICAFAETNRAPF
                     DLPECETELVGGYHTEYSSMKLGFYLFAEYINMFISSAIIASLYFGGYNIPFQSHFAL
                     DGNMLSIFQFLFFFGKIIFFIFFFMWVRWTIPRFRYDQLMNLGWKILIPLAIFNILLT
                     GVGMYAFGKV"
     gene            complement(3505..>3983)
                     /locus_tag="PLF48_10080"
     CDS             complement(3505..>3983)
                     /locus_tag="PLF48_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49717.1"
                     /translation="YRGDHAEISTYIQKAIDNEFSGNIIDVCPVGALTDKTFRFKSRV
                     WFTRPVDAHRDCPKCSGKVRLWFKGEDILRVTARKDRWGEVEEFICNECRFDKKHITD
                     WVIEGQSKINDHSVIAQNHYAKNFNAERKVFQHEVEAEKSKKAELGEGALNPNNHF"
BASE COUNT         1231 a          818 c          624 g         1310 t
ORIGIN      
        1 aatttcagat tttcttatgt taagaaatga taagtttcaa attcatgact aaaaccaaat
       61 tcaatgaatc tttagtcctg atttgtgaat ttaattctga aagttctctg gattgtttat
      121 ctatagtttc cagtgatttt ttgatactct ccgctccttc tttactgata acggacaaat
      181 tagtaagctg atccagtaat tttatatttt gcgttttctg gaaatttact tcggaattga
      241 gaccatcaat tgtacttttg gctttggtga gttcatccat caaattgtta taatcggtgg
      301 actttccggc aaatttcttt tcgcaatcag ccaagtccga agtagtctga cgcaaggttt
      361 gctcgatttt atctttttct aactgagtag cctgaaattt ctttttcgtt acgcaagaaa
      421 caagcccgaa cacagttaat aaaataaaca gctttttcat tttataattt ttatcctttt
      481 aaagtattca gcacatcaat atctgatgat tttacatttc gatagaccat catgagtatg
      541 gctagtccga cagaaacctc agcagctgca acaaccatga taaaaaacac gaacacttgc
      601 gctccgctgt cgccgaaata tcttgaaaat gcgaccatta ataaatttac ggcattcagc
      661 atcagctcaa tacacataaa aatgataatg gcattacggc ggaacatcac acctaatgca
      721 cccaaactaa acaagattac acttaagaaa atgtaatact gaatcggtat gcctataatt
      781 acttcgttca tcttaatagt tttcagtttg caaaaaatca gtttgcatca tttatttttt
      841 agttttcatt atttcaatac tacccatgta ttttaatgga tgattaatca tgtgattcaa
      901 tagtttacca acttcatttg atctttctaa aagtatttca taatctgaca agtcgataaa
      961 atttagttct ttagcaaatt ctaaccacac ttgtgtttct gtattctcca tatctgcatc
     1021 agaaatttta cttacaaaat aggcttcata ctgtcttttt ctatatcctt ctgctatact
     1081 tgcacttaca gatttagaac tgcgctttat ttgggatatg attccaaaag tttcatcttt
     1141 aggaaaccct gaacaaattt tatgtatgtc taatgccaac gtaaagctct ttttataaac
     1201 tgttaaatct ctatatgttc ccatttatca tttctgtttt gccaactgaa taattgtcaa
     1261 ttgccaactg aattcaattt tctttcttac tcaaaaatac tgctcctacc attgctgata
     1321 aaaataaaac actggacacc tcaaaaggca gcatgtattc attgaataat gtatttccta
     1381 aattctctac caagcctaca ttactgaatt tcaaagcagg caacatcgaa tgttcagccc
     1441 ccttaagtaa actgacaagg cttactaata acagacatgc tgttactact gcaataaact
     1501 tcggcaaatt ctttttatgc ggttctattt ctttgttgag attaagcatc atgatgacat
     1561 ataaaaataa aaccatgatg gctcctgcgt aaacaatgat atgtactacg gccaaaaact
     1621 gggcattaag taaaacataa tgaccggcta ttgcaaaaaa ggtaacgatc aggtataaca
     1681 cagagtgtac cggattctta tccaaaatga ccatcagcgc actgatcacc gctaagaaag
     1741 ccaaaaagaa aaatatgtat tgtgagagta acataagtta atggtgcttg ttttcttgaa
     1801 tcaaagcttg tttgtcttcc caggtttgat tgtgtttttt tgttttatct tttttaaaag
     1861 ccaacacttg aggagtttgt ctttttgaca catctatcct gttgttcata tcttctacca
     1921 gtaaatcctt gccataattc attttatttc tggtaacact taccggcatg attctgtcag
     1981 taagaaagat ggcttcttta gggcatgctt cttcacacaa tccgcaaaaa atacaacgca
     2041 gcatatttat ttcgtagact gttgcgtatt tttcttcacg ataaagatgc tcctctcctt
     2101 ttttacgctc acctgcctcc atagtaatag cttcagcagg acatgcgatg gcacacaaac
     2161 cgcacgcagt gcatctttct gctccggcat catctcgttt caaaacatgt tgtccacgat
     2221 atacgggggc aatttcgcgt ttaacttcgg gatatctgat tgttgcaggt tttttaaaga
     2281 agtgctttaa tgtaatactc ataccggcag taattgcagg caaatagagc ttctctgtaa
     2341 gagacatctc tttttttacc actaatttgc ttctgtttgt cagtttcata taatttgcaa
     2401 tttgcagaat tctgtttgca aactattcga ttgcaaactg aaaaatctga ttatactttc
     2461 ccaaatgcgt acatgcctac tccggtgagt aatatgttga atatagctaa tggaatcaga
     2521 attttccaac ccaaattcat caattggtca tatctgaatc ttggtatcgt ccatcgtacc
     2581 cacatgaaga aaaatataaa gaatattatt ttgccaaaga agaataaaaa ctgaaatata
     2641 gagagcatat taccgtcaag ggcgaaatgg gattgaaatg gtatattata tccaccaaaa
     2701 taaagcgatg caatgattgc cgatgaaata aacatattga tatattcggc aaacagataa
     2761 aatcccaatt tcatggacga atattccgta tgatagccac ccaccaattc tgtctcgcac
     2821 tccggcaagt caaatggtgc tctgtttgtc tctgcaaaag cgcaaatcac aaaaatcaaa
     2881 cagcccaagg gttgataaat aatattccag aatccttctc cttgctgagc tacaatacct
     2941 cctaaactca gcgttcctgt tttcattatc agtgcaacaa gtgccaagcc catggctatt
     3001 tcatagctaa tcatttgtga ggaagcgcgt aatgcaccta acagtgaata tttattattt
     3061 gatgcccatc ctccaatcat gataccatat acaccgaatg aaaccacacc gaatacatat
     3121 aaaataccaa tattgatatc tgtaatttgc agaggaaata cgatcccgtt tatatgtagt
     3181 ggactagccc aaggaattac gacaccggtc attaaagccg tcagcatagc caaactcggc
     3241 cctagaataa agagatattt attggatact gccggaataa tttcttcttt cataaacatt
     3301 ttcaaaccat cagcaagtgg ctgaagcacg ccataaggcc ccgctctgtc cgggccgata
     3361 cgatcttgca taaatgctgc tactttacgc tcgccgtaag tagaataagc tgctatgcct
     3421 aatgcaatca ggaagactat taaaaccaac aatatcttat acaataaaaa aacctcgatt
     3481 cccatatgct taattagttc tgctttagaa atgattattc ggattgaggg caccttcgcc
     3541 cagttccgct tttttacttt tttccgcttc tacttcatgc tgaaatactt ttcgttcagc
     3601 attaaagttt ttggcataat gattttgagc tattacagaa tggtcgttaa ttttactttg
     3661 tccttctatt acccaatctg taatgtgctt tttatcgaat ctgcattcat tacagataaa
     3721 ctcttctacc tcgccccagc ggtctttgcg ggcagtaact cgcaaaatat cttctccttt
     3781 gaaccacaat ctcacttttc ccgaacattt tgggcaatct cggtgagcat ctacagggcg
     3841 tgtaaaccaa actcggcttt taaagcgaaa tgtcttatcg gtcaaagcac ctacagggca
     3901 tacatcaatt atatttcccg aaaattcgtt gtcaattgct ttttgaatat aagtggagat
     3961 ttctgcatga tcacctctgt ata
//
LOCUS       DAOQII010000238         3975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13899_length_3975_cov_4.992602, whole genome shotgun sequence.
ACCESSION   DAOQII010000238 DAOQII010000000
VERSION     DAOQII010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13899_length_3975_cov_4.992602"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..1179)
                     /locus_tag="PLF48_10085"
     CDS             complement(397..1179)
                     /locus_tag="PLF48_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNF49718.1"
                     /translation="MHASSPLIDTHTHLYERQFQSDRDAMIQRAFAAGVSALLIPNVD
                     SSTITPMLEVCEKYPLQIFPMMGLHPCYVKPDTYIKELALVYEYLQKQKIVAVGEIGI
                     DMYWDKSTLEIQKEAFHQQCDWALEFDLPIAVHARESNYILIELLKNRGSNPSGVFHC
                     FTGSVEEAKEILKLGFYLGIGGVVTYKNTNLRDTLKHIPLENIVLETDAPYLPPVPHR
                     GKRNESAYTQLVAEMLCTVYEMSYEEIAMQTTANAKRLFKLG"
     gene            complement(1193..1825)
                     /locus_tag="PLF48_10090"
     CDS             complement(1193..1825)
                     /locus_tag="PLF48_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF49719.1"
                     /translation="MYLVTLKSQTLYFVQLPQFLNQISPKNITWCKPNTNRFVYLTFD
                     DGPHPEITPWILSELKNHDAKATFFCLGENALKYPFLIKNILDDGHTIGNHGYLHLKA
                     SWFNTKEYLHNFEKGKAVLKDVSGKDINYFRPPYGAFYMQTPAQIILWTLMPGDFDTR
                     QTKEKCLSNLLKCQSGDIIVLHDNEKSFPHLKYSLPLFLEQNTAFNFQAL"
     gene            1924..3423
                     /gene="gltX"
                     /locus_tag="PLF48_10095"
     CDS             1924..3423
                     /gene="gltX"
                     /locus_tag="PLF48_10095"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNF49720.1"
                     /translation="MSVRVRFAPSPTGTLHVGGVRTALYNYLFAKKNKGTFILRIEDT
                     DQNRFVPGAEEYIMQSLAWCGIKIDEGVAEGGQFGPYRQSDRKQLYKQYADELVKNGH
                     AYYAFDTPEELDVLRKSNANFSYNIHSRLNLKNSLVLTAEETQAKLDANQPYVIRIKV
                     PQDEEIRFNDIVRGLVTFQSEQIDDKVMFKSDGMPTYHLANVVDDHLMQITHVIRGEE
                     WLPSTPVHVLLYRFFGWDETMPQFAHLPLILKPDGNGKLSKRDGERLGFPVVPLDFEN
                     PVTHEKTSGFRDKGFLPEAFVNFLALIGWNPGTEQEIFSMDELIELFDIHKLHKSGAR
                     FDFEKAKWFNAEYIKLLPDTEIAKWLQNHQPDTYSEINLAYLTQAVGLVKERLVFLQD
                     VNHFEYLFSRPEKYDAVQSGKLSSFITAFNPDLFESDLDKIVDFNIENIEATIKNYAQ
                     EKQLKTGELMKFLRLSLVGELSGPAIPELILLFGKAETLKRIKNMMDKV"
     gene            3455..>3975
                     /locus_tag="PLF48_10100"
     CDS             3455..>3975
                     /locus_tag="PLF48_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNF49721.1"
                     /translation="MSANNFIEEIIETDLGAGKYTSILTRFPPEPNGYLHLGHAASIC
                     LNFGVAQKYGGKTNLRFDDTNPTTEETEYVESIKNDVKWLGFDWASVFYASDYFEQLY
                     QFAVRLIQQGDAYVCDLTADEIAAQKGSLTEPGINSPYRERSTEENLQLFADMRAGKY
                     KEGEKTLRAKIDMA"
BASE COUNT         1267 a          674 c          798 g         1236 t
ORIGIN      
        1 ctcgaacccg cgaccctcac cttggcaagg tgatgctcta ccaactgagc tacttccgca
       61 tattttttta aagaacggac tgcaaaaata gcatgttttt tctaaaatac aatacttcca
      121 atgtaaattt tatgagaatt tgaattatcc taacccgttg tgctttttaa aacatactaa
      181 tggtaaataa aataataaaa gtgtttggtt ggcagtatgt aaagaagcgt gggttggcaa
      241 aacgcgtagc taagcgtagg caagcaacgt gggacgattg ttttgccttg tctttttggg
      301 ttactttttt tgactaagaa aaaaagtgac ttgagtttaa tattaacaat caatggaata
      361 agaaagatga attacaaatt caaaccgcac aacggtttat cccaatttaa aaagtctttt
      421 cgcattcgct gtcgtttgca tggctatctc ttcatagctc atttcataaa ctgtacatag
      481 catttctgct accagttggg tataagcact ttcattgcgc ttaccccgat gcggaacagg
      541 aggcaaatat ggcgcatcag tttccaatac aatattttcc agagggatgt gctttaatgt
      601 atcgcgcaga ttggtatttt tataagtaac tacgccaccg attccaagat aaaacccaag
      661 cttcagaatc tctttagctt cttccacact tccggtaaaa caatgaaata cacctgaagg
      721 attgctgcct ctgtttttta gcaattctat caaaatataa tttgactccc gtgcgtgaac
      781 tgctatcggt aaatcaaatt caagtgccca gtcacattgt tgatgaaatg cctctttttg
      841 tatttcgaga gtagatttat cccaatacat atcaattccg atttcgccaa cggcaacaat
      901 tttttgtttt tgcagatatt catataccag tgcaagttct ttgatatatg tgtcgggttt
      961 tacataacaa ggatgtaagc ccatcatggg gaaaatctgt aacggatatt tttcacatac
     1021 ttcaagcatg ggtgtaatag tactgctgtc tacattaggt atcagcaatg ctgaaactcc
     1081 tgcagcaaag gcacgctgta tcatggcatc tctgtcggat tgaaactgac gttcgtataa
     1141 atgagtgtgt gtatctatta gtggtgatga agcatgcata agggttgtaa atttataatg
     1201 cctgaaagtt aaaggctgtg ttttgttcta aaaacaaagg taaactatat ttcagatgcg
     1261 gaaaactttt ttcattatca tgcaatacaa taatatcacc cgattggcat tttaataaat
     1321 tagataaaca tttttctttc gtctgccggg tatcaaaatc gccgggcatc agcgtccaga
     1381 gtataatttg tgcaggagtt tgcatgtaaa aagctccata gggaggtctg aaatagttga
     1441 tgtctttccc tgaaacatct tttagcacag cttttccttt ttcaaaatta tgtaaatatt
     1501 cttttgtgtt aaaccaactt gcttttaagt gtaaataacc gtgattacca attgtatgtc
     1561 catcgtccag aatatttttt attaaaaacg gatatttgag cgcattttca cctaagcaaa
     1621 aaaaagtggc ttttgcatca tgatttttta attcggataa gatccacggt gttatttcgg
     1681 gatgcggacc atcatcaaat gttagatata caaagcggtt agtattaggt ttgcaccatg
     1741 taatattttt tggtgaaatc tgatttaaaa actgtggcaa ttgtacaaaa tacaaggtct
     1801 gtgatttgag tgttacaaga tacaaaataa caatttctga aaaatatcaa ttaaaattaa
     1861 actgacattg cgctaaactg ccttatttct tagtattttt gaggtttgtt aaaaattcaa
     1921 tttatgtctg ttcgcgttcg ttttgctcca agtcctaccg gtacattaca tgtcggaggt
     1981 gtccgtacgg ctttgtataa ttatttgttt gcaaaaaaaa acaagggaac ttttatactc
     2041 cgtatcgaag ataccgatca gaaccgattt gtacccggtg cggaggaata tatcatgcaa
     2101 tcactggcat ggtgtggaat aaagatagat gaaggtgttg cagaaggcgg tcaatttggt
     2161 ccctatcgcc aaagcgacag aaagcagttg tacaagcagt atgccgatga attggtgaaa
     2221 aacggacatg cttattatgc tttcgatacg cccgaagaac tggatgtatt gcgtaaatca
     2281 aatgcaaatt tttcatacaa tatacatagc aggttaaatc tcaaaaacag tctggtgctt
     2341 actgcagaag aaactcaggc aaaactggat gcaaatcagc cttatgttat tcggataaaa
     2401 gtaccgcaag atgaagaaat tcgatttaat gatatcgtgc ggggtttagt aacttttcaa
     2461 tcagaacaaa ttgatgataa agtgatgttt aagagtgacg gtatgcccac ttatcatttg
     2521 gcaaatgtgg tagatgatca tctcatgcag attacccatg tcattcgtgg tgaagaatgg
     2581 ttgccgtcaa cacctgtaca tgtattgctc tatcgctttt ttggatggga tgaaacgatg
     2641 ccacaattcg cacatttgcc cttgatatta aaaccggacg gaaacggaaa attatcgaaa
     2701 agggatggag agcgtttagg ctttccggta gtgccgcttg attttgaaaa tcctgtgaca
     2761 catgagaaaa cttccggatt tagagataaa ggatttttgc cggaagcgtt tgtgaatttt
     2821 ctggctttga ttggatggaa tcccggtacg gagcaggaaa ttttcagtat ggatgaatta
     2881 attgaattgt ttgatattca taaactgcat aaaagcggag cccgcttcga ttttgaaaag
     2941 gcaaaatggt ttaatgcaga atatataaaa ttactgcccg atacggagat cgcgaaatgg
     3001 ttgcaaaatc atcagcctga tacatattca gaaataaatc ttgcctattt aacacaagct
     3061 gttggattag ttaaagagcg attggttttt ctacaggatg tcaatcattt cgagtatttg
     3121 tttagtagac ctgaaaaata tgatgctgta cagtcaggaa aattaagttc atttataacc
     3181 gcttttaatc cggatttatt tgaatcagat ttggataaaa ttgtagattt taatatagaa
     3241 aatattgaag caacaattaa aaactatgcg caagaaaagc aactgaaaac cggagagctg
     3301 atgaagtttt tgcgactgag tttggtaggt gaactttctg gtcccgcaat tcccgaatta
     3361 attttgctct ttggaaaagc tgaaacacta aaacggataa aaaacatgat ggacaaagtc
     3421 taattcccaa tcactaatca cttaaagaaa caacatgtca gcaaataatt ttatagaaga
     3481 aataattgaa acggatttag gagccgggaa atatacatct attttaacgc ggtttccacc
     3541 cgagcctaat ggctatttac atttaggaca tgcggcatcc atttgtctta attttggagt
     3601 ggcacaaaaa tacggaggca aaaccaattt gcgttttgat gataccaatc ctaccaccga
     3661 agaaaccgaa tatgtagaaa gtataaaaaa tgatgtgaaa tggttgggtt ttgattgggc
     3721 gagtgttttt tatgcatctg attattttga acaattatat cagtttgccg ttcggctgat
     3781 tcagcaagga gatgcgtatg tttgtgattt gacagcagat gagattgctg cacaaaaagg
     3841 ctctttgaca gaacccggaa taaattctcc gtatcgtgaa cgcagcacag aagaaaacct
     3901 tcaattattt gctgatatgc gtgccgggaa atataaagaa ggcgaaaaaa cattacgcgc
     3961 taaaattgat atggc
//
LOCUS       DAOQII010000239         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13949_length_3965_cov_4.695652, whole genome shotgun sequence.
ACCESSION   DAOQII010000239 DAOQII010000000
VERSION     DAOQII010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13949_length_3965_cov_4.695652"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLF48_10105"
     CDS             <1..296
                     /locus_tag="PLF48_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNF49722.1"
                     /translation="VRFGRMEHRFQESTFSTLVELIEKNTGRKISAKEKEIIHGAQEI
                     DIVRSGLEDTMIHSYEAIHEIWKKKKIKTIRTAAFVNALNKIGTAYLELGVFP"
     gene            307..699
                     /locus_tag="PLF48_10110"
     CDS             307..699
                     /locus_tag="PLF48_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF49723.1"
                     /translation="MKKILLLIGIGASFLLFAQDKTKPQFPGGEQQLVAFLSKNISYP
                     PEANKNRWQGKTLIAFFVNTDGSLSDFAVQKSSWPVLDAEALRVVKLMPKWIPATENG
                     KTIKEKVVLPVMFNLKMRDKGNNNQVSE"
     gene            696..1472
                     /locus_tag="PLF48_10115"
     CDS             696..1472
                     /locus_tag="PLF48_10115"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNF49724.1"
                     /translation="MKSKDEIVKNWLPRYTGRALEDFGEYIILTNFSNYIHLFASWNQ
                     VEVMGLDKPMQTATADNITIINFGMGSPLAATMIDLLSAIQPKAAIFLGKCGGLKKKN
                     EIGDFVLPIAAIRGEGTSNDYFPPEVPALPSFALQKAISTTIREFQQDYWTGTVYTTN
                     RRVWEHDEEFKEYLRKIRAMAIDMETATLFSVAFANEIPIGALLLVSDQPMIPEGVKT
                     EQSDKAVTSNYVELHIKIGIESLRQLMNRSTTIKHLRFED"
     gene            complement(1469..2161)
                     /locus_tag="PLF48_10120"
     CDS             complement(1469..2161)
                     /locus_tag="PLF48_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNF49725.1"
                     /translation="MPGIYLISILIFGVSFLASTMLKRRFQQYSEIPLNLTGKEVAER
                     MLYENGIHDVKVVAVGGALTDHYNPATKTVNLSDWVYAQANVAAAAVAAHECGHAVQH
                     ATAYNWLTMRSKLVPVVQFSSNIVQWIIMGGLAVLAFTKGQVPWVLLLGIILFAITTL
                     FSFITLPVEFDASARAIQWIDRSGIMPSKDLGKAKNALNWAASTYVIAALGSLVTLLY
                     YISIFMGGRRRD"
     gene            complement(2166..2453)
                     /locus_tag="PLF48_10125"
     CDS             complement(2166..2453)
                     /locus_tag="PLF48_10125"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNF49726.1"
                     /translation="MLVYNITFKVDLDSSVSFLDFLEQYTAEHALDFELFQLANVDDS
                     DGFTYCLHQKFSDLGTYNRHIVVIDRKFKEQIIEKYGDKVLFFGSILNKVK"
     gene            complement(2541..2879)
                     /locus_tag="PLF48_10130"
     CDS             complement(2541..2879)
                     /locus_tag="PLF48_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49727.1"
                     /translation="MTKINIPELNRDVVIWRSLVELKNTSNDDVAVNRPSYFGYYYAY
                     LNPMQIEIVQHAVPQYVLATAYKNYVVEKVKMLEANQSLLSEKYYATFDNIDLNNEIA
                     AWDMSYIFNR"
     gene            3146..3721
                     /locus_tag="PLF48_10135"
     CDS             3146..3721
                     /locus_tag="PLF48_10135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNF49728.1"
                     /translation="MNQNAYIQDIAQALHNGKTILYPTDTIWGLGCSALNEVAVNQVF
                     SIKNRPKQKSMILLVSDWEMLARYIHLPKLLSEELQHLLQESTVPTTIIFPYTQHLPK
                     FILAENGSIGVRIPNHRFCHQVIRYLDAPLVSTSANLSGQTSPFQFSDISDDIKKQVD
                     IIVDSAFDTSDYKKPSRILLLSEQGEIEEIR"
BASE COUNT         1313 a          745 c          750 g         1157 t
ORIGIN      
        1 acgtgcgttt cggccgtatg gaacaccgtt ttcaggaatc aacattttct accttggtag
       61 aattgattga aaaaaataca ggtagaaaaa tttcagccaa agaaaaagaa atcatacatg
      121 gtgcacaaga aattgatatt gtacgttcgg gattggaaga cacgatgata cattcttatg
      181 aagccatcca cgagatttgg aaaaagaaaa aaatcaaaac tatacgtacg gctgctttcg
      241 tgaatgcact caataaaatc gggacagctt atttggaatt gggcgttttc ccttaaaaat
      301 cgaaacatga aaaagatatt gcttttaata ggaataggtg cttctttctt actctttgca
      361 caagataaga ccaagccaca attcccgggt ggagaacagc aactagtagc ttttctttct
      421 aaaaatatca gttacccgcc tgaggcaaat aaaaatagat ggcaaggcaa aacattgatt
      481 gcattctttg taaatacaga tggcagcctt agtgatttcg ccgttcaaaa atcatcatgg
      541 cccgtgttag atgcagaagc attacgcgtg gtaaaattga tgccgaaatg gataccggcc
      601 acagaaaatg gaaaaacaat caaagaaaaa gtagtgctgc cggttatgtt taatttgaaa
      661 atgcgggata aaggaaataa caatcaggta agcgaatgaa atctaaagat gaaatagtaa
      721 aaaactggct gcctcgttac actggcagag cattagagga ttttggagaa tatattatcc
      781 tcacaaattt ttccaattat attcatcttt ttgcttcctg gaatcaagtt gaagtaatgg
      841 gtttagataa acccatgcaa acggctactg cagataatat cactattata aattttggca
      901 tgggaagccc gcttgcagcc acgatgatag atttactctc agctatccag ccaaaggcag
      961 caatattttt aggcaaatgc ggaggattga aaaagaagaa tgaaattggt gattttgtgt
     1021 tgcccattgc agctatcaga ggtgagggca cttccaatga ttatttcccg cccgaagtgc
     1081 cggctttgcc gtcatttgcc ttgcaaaaag caatttctac taccatcaga gaattccagc
     1141 aagactactg gacgggaacg gtttatacca ccaaccgtcg ggtatgggag catgatgagg
     1201 aatttaaaga atacttgcgc aaaatacgtg ctatggccat tgatatggaa actgccacgt
     1261 tattttctgt agcctttgcc aatgaaattc cgataggtgc attattgctt gtttccgatc
     1321 agcctatgat accggaaggg gttaaaactg aacaaagcga taaagcagtt acgtctaact
     1381 atgtagaatt acacataaaa ataggcatcg aatccttgcg tcaattgatg aatcgcagca
     1441 caacaatcaa gcacctgcgt tttgaagatt agtcccttcg gcgaccaccc ataaagatag
     1501 aaatgtaata cagcaaggtt actaatgagc ctaatgctgc gattacatag gttgatgctg
     1561 cccagtttaa tgcattttta gctttaccca aatctttaga tggcattata ccggatctgt
     1621 ctatccactg tatggcacgt gcagaagcat caaactctac aggcaaagta ataaagctga
     1681 agagtgtagt aatggcaaaa agaataatac ctaatagcag aacccaaggc acttgacctt
     1741 tagtaaatgc caaaacagcc aacccgccca tgataatcca ctgcactata ttagaactga
     1801 attgtactac tggcactaat ttggagcgca tggtcaacca attataagca gtagcatgtt
     1861 gcacggcatg tccacattca tgagcagcca ctgcagcagc agctacatta gcttgtgcat
     1921 atacccagtc gctcaggttt acagtttttg ttgccggatt gtaatggtcg gttagagcac
     1981 cacctacagc aaccactttg acatcatgaa tgccattttc atacagcatt cgctctgcta
     2041 cttcttttcc ggtcagattc aacggaattt cagaatattg ctggaaacgg cgtttcagca
     2101 tggtgctggc aagaaaacta acaccgaaaa tcaagataga aataagataa attcctggca
     2161 tataattatt ttactttgtt taaaatgcta ccgaaaaata agaccttatc gccatatttt
     2221 tcaataattt gttccttaaa tttcctgtct ataacaacaa tgtgccggtt ataagttccc
     2281 agatccgaaa atttttgatg cagacaatag gtaaatccgt cactgtcatc cacattggct
     2341 aattggaata attcaaagtc aagagcatgt tccgccgtgt attgttctaa aaaatcaaga
     2401 aatgaaacgc tactgtccaa atcgacttta aacgtgatat tataaacgag cataacacaa
     2461 aggtaaaaaa aagagagtaa aaaaaatttg gcggcgccga aggcgccgcc aaatgatctc
     2521 attccccaat gatactgatt ttatctgtta aagatataac tcatgtccca tgcagcaatc
     2581 tcattattca agtcaatatt gtcgaaagtt gcataatatt tctcactcag taagctctgg
     2641 tttgcttcca gcatttttac tttttctacc acgtagtttt tataagccgt tgccagtaca
     2701 tattgtggta cggcatgctg cacaatttca atttgcatag ggttgaggta ggcatagtaa
     2761 tacccaaaat aactggggcg gtttactgcc acatcgtcat tactggtgtt ttttagctcc
     2821 accagactcc tccaaatcac cacatcacgg ttgagttccg ggatgttaat ctttgtcact
     2881 ctggtaattt gctgcctgaa aataattcct tctcgcttta ttacaggtga agcagcaaaa
     2941 ctataagaaa ttaagaaaaa acttaatata aaaattgatt ttttcattat taaacttttc
     3001 attatagttg ttatttaaac taaaagcgtt gcatgtacca tatattttat tttacagttt
     3061 ttcgataaaa ttgatgtgag tcaaaactaa aatagttagt attaaacctt taatatgtaa
     3121 acctttatct ttgatgatat tgtttatgaa ccaaaatgca tatatacagg atatcgctca
     3181 ggctcttcac aatgggaaaa caatattata tcctacggat actatttggg gacttggttg
     3241 ctctgcatta aatgaagtag ctgtaaatca ggtattttcc ataaaaaaca gaccaaagca
     3301 aaagagcatg atcttactgg taagcgattg ggagatgctt gcaagataca tccatttgcc
     3361 gaagctgtta agtgaagagc tgcaacactt attacaggaa tcaacagtgc ctacaacaat
     3421 aatttttcct tatactcaac atctgccaaa atttatactg gcagaaaatg ggagtattgg
     3481 agtacgtatt cccaatcatc gtttttgtca tcaggtaatc agatatcttg atgctccttt
     3541 ggttagtaca tcagcaaatt tatccgggca aaccagtccg tttcagtttt cagacattag
     3601 tgacgatatt aaaaagcaag tggatattat cgtagattct gcttttgata catcagatta
     3661 taaaaaacct tcgcgtattc ttctcctgag tgaacaagga gaaatcgaag aaatccgttg
     3721 acaaaatttg attttgaaca aataatttac tatgtacttg tgaatatgaa aatatttaca
     3781 tatctttgta tgaacatttt acaatatgca aacagtccaa ttaccttttt tagaagacga
     3841 taagctggag aaagctgctg ttattttaaa aacaattgct catccggctc ggctggctat
     3901 aataaatatg ttgcgcggca acaataaaat gagtgtgaat gatatttgcg aaaccttgga
     3961 aatgg
//
LOCUS       DAOQII010000240         3959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_13985_length_3959_cov_4.511014, whole genome shotgun sequence.
ACCESSION   DAOQII010000240 DAOQII010000000
VERSION     DAOQII010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13985_length_3959_cov_4.511014"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PLF48_10140"
     CDS             complement(<1..1237)
                     /locus_tag="PLF48_10140"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNF49729.1"
                     /translation="MSKKQDKETPLMGQYNRIKQKYPDAILLFRVGDFYETFGADAVK
                     AAEVLGIVLTKRANGSQADLELAGFPYHAVDTYLPKLVKAGYRVAICEQLEDPKMTKT
                     IVRRGVTELITPGVAINDKILDHKSNNFLASVFFKDENCGIAFLDISTGEFLVAEGNE
                     AYLDKLFQSFSPTEVVFPKYQQKRFAGLYANKYYTFAIDDWAYQDNYLEQKLLSHFQV
                     QTLKGFGLDEVHSGIIAAGACLHYLAEAEHKNIRHITSIARIQNENYVWIDRFTARNL
                     ELISSLHEKGVPLIQILDKTISPMGARLLKRWIVLPLKDITLINERLDAVEYLIREQD
                     KLHELQDYIKQIGDLERLISKVPLGKINPREVIQLQRALTAIVPIKEICQNSQNNGLR
                     KIGEQLQPCSFIKDKIQQTI"
     gene            1487..3856
                     /locus_tag="PLF48_10145"
     CDS             1487..3856
                     /locus_tag="PLF48_10145"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNF49730.1"
                     /translation="MKNLLFTLLALATWFSGAQTTFAQNSKSIVLCETYNSSDGTPSG
                     VGASWDIDKSTGGNVYVVYRNGSSKITENLLLYVDKLNSSSKYVAYATENLSLGANKK
                     WAMFDFKFTESGKYKLSAVTPTGSTLASVYADINYKNSYSTTDNDDNSSSIYESLQLS
                     DNSSSSSGKSIVLCESYNDYGKATGIYSTWNIKSVGGFVYILYNNNGKNIKNTLWLYV
                     DKKNENGKYVPFETKTFDINKNKSWSAYDFKFTETGNYKISAVSSNGEEAVTYATIKL
                     ADGEKEKTEKTIVTCESYDDYGKPVGKGNEWDITADKGYVYILFDNGEPITNSVSLFI
                     DKKNAKGEYAIYDTKTFETSDSKDWSVYDYKFKEGGDYKLSAYSNGEYVATTFVKMNY
                     KTSSSSTSSTTSTKDKVDTWYYENSTVSFADNADANGKMSNSNTTFFLSGGKKSIAVV
                     YKDAKAIKTTKVYMDVYSIGTDNQEDFIETITLYTSATQKVCVGNYTFKNSGTYKVSF
                     YNADNIFMNMGYVTVLGSISESTTKYTNTNSGSSTQTYLNSLMLPESSTSDNNPRVAL
                     VIGNGNYVSSGTLPNPVHDAEEMSKALKNCGFTVTTVINGNKKQMNEAVNKFGQMLSS
                     NKNATGMVYYAGHGIQSKGENYIIPIDAEIGVEEDLQYECLNVGKILAKMEAAKNPIN
                     IIALDACRNNPFERSWNRSTSNGNGLTGMNAPEGTIISFSTNPGNVASDGDGYNSPYT
                     TAFLNQLKKSGLSIEQVLKGVAAEVKKNQPGQLPWYSSSIVGEFYFRAK"
BASE COUNT         1331 a          712 c          729 g         1187 t
ORIGIN      
        1 caatagtttg ctgtatttta tctttaataa aactgcaagg ttgcaattgt tcaccaattt
       61 ttcgcaaacc gttattttgt gagttttggc aaatttcttt tatgggcaca atagccgtta
      121 atgcccgttg caattgtatg acttctctcg ggttaatttt acctaagggt actttagaaa
      181 tcaaacgctc taaatcgccg atttgtttaa tgtaatcttg cagctcgtgc aatttatctt
      241 gttctcgtat caaatattct accgcatcta atcgttcatt aatgagcgta atgtctttta
      301 atggcaaaac tatccaacgc ttcagcaagc gtgctcccat cggagaaatc gtcttatcta
      361 atatttgaat aagtggtaca cctttttcat gcaaagaaga aatcagctct aaattgcgtg
      421 cggtaaaacg gtctatccag acatagtttt cattttgaat gcgggcaatg gaagtaatat
      481 ggcggatatt cttatgttca gcttcagcca gatagtggag acaagcacct gctgcgatga
      541 taccagagtg tacttcatcc aaaccaaaac ctttgagtgt ttgcacctga aaatgcgaga
      601 gcaatttttg ttctaaataa ttatcctgat aagcccagtc gtcaatggca aatgtgtaat
      661 atttgttggc atacaatccg gcaaaacgct tttgctggta ttttggaaat actacttctg
      721 tcggagaaaa actttggaat agtttatcta aatacgcttc atttccttct gccactaaaa
      781 attcgcctgt ggaaatatcc aaaaatgcaa taccgcaatt ttcatcttta aaaaaaacgg
      841 acgctaaaaa attattggac ttatgatcta atattttatc attgatagca actcccggtg
      901 ttattagttc cgttactcca cgcctcacga tggttttcgt cattttcggg tcttccaact
      961 gttcgcatat cgctacacgg tatccggctt ttaccaattt gggtaaataa gtatctactg
     1021 catgatacgg gaagcctgcc agttccaaat ccgcttgcga gccgtttgct ctcttcgtta
     1081 aaacgatgcc taatacttct gcggctttaa ccgcatctgc cccaaatgtt tcgtaaaaat
     1141 cgccaactcg aaaaagcaaa atcgcatccg ggtatttttg tttgatacgg ttgtattgcc
     1201 ccatcagtgg cgtttcttta tcttgttttt ttgacattgc cttttgcgta ctttttaacc
     1261 tattttactc atttcaaata agaaaataaa aaaagaattt tttatcataa ttgcagacga
     1321 aattatttct ccttaaaaag caaaaataca atataagcct tgtacaatat gcgtttacaa
     1381 aagcatctta tccacaagca aaatgatgca ttattgaaaa aataccataa ttcacgtatg
     1441 gcgcattaaa aaaaatctgt atattttagc attaaaaatt atcactatga agaatttact
     1501 gtttacgtta ctagcattgg caacatggtt ttcgggtgct cagactacct ttgcccaaaa
     1561 cagcaaaagt attgtattat gtgaaaccta taattccagt gatggcacac caagcggtgt
     1621 tggtgcttca tgggacattg acaaaagcac gggaggcaat gtatatgttg tttatagaaa
     1681 cggaagttca aaaattacgg aaaacctatt gctttatgta gacaaactta acagcagcag
     1741 caaatacgtt gcttatgcta ctgagaacct gagtttgggc gcaaacaaga aatgggccat
     1801 gtttgatttc aaatttaccg aaagcggaaa atataaatta tcagcagtta cacccactgg
     1861 ttctacgctg gcatcggtat atgctgatat caactacaaa aattcatata gcaccactga
     1921 caatgacgac aatagcagct ctatttatga gagccttcaa ttatctgaca attcaagctc
     1981 ttcatccggc aaaagcatcg ttttatgtga gagctataat gattacggca aagccaccgg
     2041 catttattct acttggaata tcaaaagcgt tggagggttt gtttatattc tatacaataa
     2101 caatggtaaa aacataaaaa acaccctatg gctatatgta gataaaaaga atgaaaatgg
     2161 gaaatatgtg ccatttgaaa cgaaaacatt tgatatcaac aaaaacaaat catggtcggc
     2221 ttatgatttt aaatttacgg aaacgggaaa ttataaaatt tcagcagtaa gttctaacgg
     2281 cgaagaggcc gtaacttatg ccactattaa gctggctgat ggagaaaagg aaaaaacaga
     2341 aaaaactatt gtaacctgcg aatcttatga tgattatgga aagcctgtag gcaaaggtaa
     2401 tgaatgggat attaccgccg ataaagggta tgtctatatc ctattcgaca atggagagcc
     2461 tataacaaat agtgtatcgt tgtttatcga taagaaaaat gctaaaggcg aatatgcaat
     2521 ctatgacaca aaaacttttg aaaccagtga tagtaaagat tggtctgttt atgattacaa
     2581 atttaaagaa ggcggtgatt acaaactcag tgcatatagc aatggcgaat acgttgcgac
     2641 cacttttgta aagatgaact ataaaacatc cagttcttcc acatcttcca ctactagtac
     2701 taaggacaaa gtggacacct ggtattatga aaattcaacc gtaagttttg cagacaatgc
     2761 ggatgccaat ggaaaaatgt cgaatagtaa tacgacattc tttttatcag gtggcaagaa
     2821 aagtattgcg gtagtataca aagatgcaaa agcaataaaa acgacaaaag tgtatatgga
     2881 tgtgtatagt atcggtaccg acaatcagga agattttatt gaaaccatta ctttgtatac
     2941 atcagctact caaaaagtat gcgtaggtaa ctatacattt aaaaattcgg gcacttacaa
     3001 agtatcattc tacaatgctg acaatatttt catgaatatg gggtacgtaa ccgtgttggg
     3061 ttcaataagt gaatcaacta cgaaatacac taatacaaac tcagggtcaa gcacacaaac
     3121 atatctgaac agtttaatgt tgccagaaag ttcaacatcc gacaataatc cacgggttgc
     3181 cttagtaatt ggtaatggaa attatgtatc atccggaaca ctgcctaatc ctgtgcacga
     3241 tgcagaagaa atgtcaaaag ctttgaaaaa ttgcgggttt acagttacaa cagtaattaa
     3301 tggtaataaa aaacaaatga atgaagcggt aaataaattt ggtcaaatgc tgtcatccaa
     3361 taagaatgct accggtatgg tgtactatgc cggacatggt attcagtcaa aaggagaaaa
     3421 ttatatcata ccaattgatg ctgaaattgg cgtagaagaa gatttacaat atgaatgtct
     3481 gaatgtaggc aagatattag caaaaatgga agctgctaaa aacccaataa atataattgc
     3541 acttgatgcc tgccgtaata acccttttga acgcagctgg aatcgttcta catcaaatgg
     3601 gaatggactt acgggtatga atgctcctga aggaacaata atttcgtttt ctactaatcc
     3661 cggtaatgta gcttctgatg gggatggtta taattcgcca tatacgactg catttttaaa
     3721 ccaacttaaa aaatcaggac tctctattga acaagtatta aaaggagttg ccgcagaagt
     3781 aaagaaaaac caacccggac agttaccttg gtatagttct tccatcgtag gtgaattcta
     3841 tttcagagct aagtaattgc ttttatttat ctgagttcgg gataagcgtt tcaattaaaa
     3901 taatagttga ttttttatac agaaccacct ccttttaact cctccacctt gttaaagtg
//
LOCUS       DAOQII010000241         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14053_length_3947_cov_4.666495, whole genome shotgun sequence.
ACCESSION   DAOQII010000241 DAOQII010000000
VERSION     DAOQII010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14053_length_3947_cov_4.666495"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLF48_10150"
     CDS             complement(<1..595)
                     /locus_tag="PLF48_10150"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HNF49731.1"
                     /translation="MKKYFSLFVFILSITFANAASTNTYDKQFDTYKGNFILRLWKQY
                     PEWATAIGFHKYDSVLNVPSQKKIQQDNAFTLKEKSKLAVFNVNKLSDLNKIDYYLID
                     NFLNKCIWENNQLKRWEWNPADYNVGEGFAYILSENYAPLKKRVSDLYMKMKNVPAYY
                     EAAKANIKNPSVEHLNLAIDQNEAIISIFENDFADSVK"
     gene            679..1578
                     /gene="hisG"
                     /locus_tag="PLF48_10155"
     CDS             679..1578
                     /gene="hisG"
                     /locus_tag="PLF48_10155"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNF49732.1"
                     /translation="MVYSLFLIQYVILGMTKIKLAIQKKGRLSDESIALINECGINFP
                     SSIGKLIATCYDFPMEILFLRDDDIPDYVQDGVADIGIVGENVLIESGKEVDEMMKPG
                     FGKCRLSLAVPRGMDYQSIVDIRGKSIATSYPKILQNFLDKQHIEAEIHVISGSVEIA
                     PGIGLADAICDIVSTGSTLLSNGLKEVEQIFQSEAVLIVNKHLSVEKSELLNQLIFRL
                     QAVKKAKKNRYILLNAPNEKLTEIIQLLPGIKSPTVLPLAQSGWSSVHSVITETDFWR
                     IIHALKNAGAEGILVMPIEHIID"
     gene            1604..2893
                     /gene="hisD"
                     /locus_tag="PLF48_10160"
     CDS             1604..2893
                     /gene="hisD"
                     /locus_tag="PLF48_10160"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNF49733.1"
                     /translation="MQTAKYPEKGLEWTSIMERPIWNRQELSLQVQPIINEVFNKGDE
                     AVFHYTALFDGVELEDLNVYEDEVEVAIQQVSQELRDAIKSAYDNIYKFHASQQEEIK
                     KIETNTGVECWRKSVPIESVGIYIPGGSAPLFSTVLMLAIPAQIAGCRQVILCSPPNE
                     NGEIHPAILYAAELCGVHEIYKVGGAQAIAAMAYGTESIPKVHKIFGPGNQYVTIAKQ
                     LVNVSGTAIDIPAGPSEVLIIGDDEARPDFIAADLLAQAEHGEDSQVIFVTTSEAHLN
                     MVHFEIDKQLVHLPRKAIAEKALKNSWMVLLNDLEDAITFSNQYAPEHLILQTRNAED
                     LAEKVINAGSVFIGHYSPESAGDYASGTNHTLPTNGYAAAYSGVSLDSFVKKITFQII
                     SKEGLEKLANTIEIMAAAEGLDAHKNAVSIRIRIDEG"
     gene            3095..>3947
                     /locus_tag="PLF48_10165"
     CDS             3095..>3947
                     /locus_tag="PLF48_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF49734.1"
                     /translation="MINIHDLVCPHVKNMKAYSSARMEYSGKEGIFLDANENSLGSVS
                     SEIHNRYPDPMQLELKAELVKHYPAYIEQLFLGNGSDECINVLIQCFCIPGQDSILQF
                     PPTFSMYEHASHVMNVKVDEILLHPQTFQLERAKVKAYVESHLHCKIIFLCSPNNPTG
                     NLLNSDDIKFILENFKGIVVVDEAYQDFAGNVSWLHSLQDYPNLVVINTFSKAWGMAD
                     IRLGMLFANEEIIRYMNTIKMPYNISQHTINTAIEALHKTIKKHAFSYLFIFIILVVF
                     VILFILIV"
BASE COUNT         1316 a          678 c          718 g         1235 t
ORIGIN      
        1 atttaacaga gtctgcaaaa tcgttttcaa aaatggaaat gatggcttca ttttgatcaa
       61 tagccaaatt cagatgttct actgaaggat tttttatgtt tgccttcgcc gcttcgtaat
      121 aagccggaac atttttcatt ttcatataca aatctgaaac tcgttttttt agcggtgcat
      181 aattctcaga taagatataa gcaaaacctt cacctacatt ataatctgcc ggattccatt
      241 cccatctttt caattggtta ttttcccata tgcatttatt caaaaaatta tcaataagat
      301 agtagtcgat tttatttaaa tcagacagtt tgttcacatt aaaaactgct agtttgcttt
      361 tttcttttag ggtaaaggca ttgtcttgtt gtattttctt ttgcgacgga acattcaata
      421 ctgaatcata tttatgaaaa ccaatcgcag tagcccattc cggatattgt ttccacaaac
      481 gcaaaataaa gtttcctttg tacgtatcaa attgtttgtc gtaagtgttc gtacttgctg
      541 catttgcaaa tgttattgat aaaatgaaaa caaacagact aaaatatttc ttcatactcg
      601 aatttttatc caaaggtacg attttcatca ggtatgcgaa atggcatttt agcaagttta
      661 attttatgtc aaacttttat ggtttactct ttattcctga ttcaatatgt aattttaggt
      721 atgactaaaa taaaactggc catacaaaaa aaaggtagac ttagtgatga atcaatagca
      781 ttaattaatg aatgcggtat taatttccca agcagcattg gtaaacttat tgcaacttgt
      841 tacgattttc caatggaaat tttgtttttg cgagatgatg atattccgga ttatgtgcaa
      901 gacggtgtgg cagatatagg catagtagga gagaatgtac tgatagagtc tggtaaagaa
      961 gtagatgaaa tgatgaaacc cggttttggg aaatgccgtt tgtcattagc tgtacctcgt
     1021 ggtatggatt atcagtcaat agttgatatc agaggaaagt ctattgccac ttcttatcca
     1081 aaaattttac aaaacttttt agacaagcaa catatcgaag cagaaattca tgttatttcc
     1141 ggttcagtgg agattgcacc cggcattggt ttagcagatg ccatttgtga tattgtttct
     1201 acaggctcta cactattaag caatggactt aaagaagtgg aacaaatatt tcaatcagaa
     1261 gctgtattaa ttgtcaataa gcatttatcc gtagaaaaaa gtgaactttt aaatcaactg
     1321 atattcagat tgcaagctgt aaaaaaagct aagaaaaaca gatatatttt attgaatgca
     1381 ccaaatgaaa aattaactga aatcattcag cttctgcccg gtataaaaag cccaacagtt
     1441 ttgcctttgg cacaaagtgg ttggagttcc gttcattctg ttattacaga aactgatttc
     1501 tggaggatta tacatgcact taaaaatgca ggtgctgaag gcatattggt tatgccgata
     1561 gagcatatca ttgactaaat catatatata aaaacttaac attatgcaaa cggcaaaata
     1621 ccccgaaaaa ggactggaat ggacaagtat tatggaacgc cccatatgga acagacagga
     1681 attgtcgctg caggttcaac cgataattaa tgaagtattc aacaaaggag atgaagcagt
     1741 atttcattat accgccttat ttgacggcgt agaattagaa gatttaaatg tttacgaaga
     1801 tgaagtagaa gtggccatac agcaagttag ccaggaattg cgagatgcca tcaaatcggc
     1861 ttacgataat atttacaagt ttcatgcaag tcaacaagag gaaataaaaa aaatagagac
     1921 caatacaggc gttgagtgct ggcgaaaaag cgtacctatt gaaagtgtag gaatctatat
     1981 tcccggcgga agtgcacctt tattttctac ggtgctaatg ctggctattc cggcacaaat
     2041 tgcaggttgt cgtcaagtca ttttatgttc tcctcccaat gaaaatggag aaatacatcc
     2101 tgcaatttta tatgctgcag aattatgcgg tgttcatgaa atttataaag taggcggtgc
     2161 acaagcaata gctgctatgg cttacggcac ggaaagtatt cctaaagtgc ataaaatttt
     2221 tggtcccggt aatcagtatg ttactattgc caaacaactg gttaatgttt cgggaacggc
     2281 tattgatatt ccggccgggc caagtgaagt tttgataatt ggtgatgatg aagcacgtcc
     2341 tgactttatt gctgccgact tattggcaca agcggagcac ggtgaagaca gccaggttat
     2401 ttttgttact accagcgaag ctcatttgaa tatggtgcat tttgaaatag acaaacaact
     2461 ggtgcattta cctcgtaaag ctattgcaga aaaagcatta aaaaatagct ggatggtctt
     2521 gttaaatgat ttagaggatg ccattacctt tagcaatcaa tacgcaccgg agcatttaat
     2581 tttgcaaacc cgcaatgcag aagatttagc cgaaaaagta atcaatgccg gatctgtttt
     2641 tatcgggcat tattcaccgg aaagtgcagg agattatgcc agtggaacga accatacctt
     2701 acccactaat ggctatgctg cggcatacag tggtgtatct ttagatagct ttgtgaaaaa
     2761 aattactttt cagataattt caaaagaagg cttagaaaaa ctggcaaata cgattgaaat
     2821 catggcagca gcagaaggtt tagatgcaca taagaatgca gtttctataa gaataagaat
     2881 cgatgaagga tgaatgctta atgcttaatg atgaatttta aattttaaat ttttttcaat
     2941 acttaatcca ccaattaaaa attaccaatt actaataacc aataaccaat aaccaataac
     3001 caataaccac ctattctcta cttaatactt aatacttaat acttaatact taatacttaa
     3061 tacttaatac ttaatactct caactcacta tcacatgata aacatacacg atttagtttg
     3121 cccgcatgtg aaaaacatga aagcttattc atctgcccga atggaataca gtggcaaaga
     3181 aggcatattt ttagatgcca atgaaaacag tttgggctca gtttcgtctg aaattcataa
     3241 ccgttatccc gaccccatgc agctggaatt aaaagcagaa ttagtaaaac actatcctgc
     3301 atatatcgag caattgtttt tgggtaatgg cagtgatgaa tgtatcaatg tgctaataca
     3361 atgtttttgc atacccggac aagatagcat tttgcaattt ccacctactt tctccatgta
     3421 tgaacatgca tcacatgtaa tgaatgtcaa agtggatgaa atcttattac atcctcaaac
     3481 cttccagcta gaacgtgcaa aagttaaagc atacgttgaa tcgcatctgc attgtaaaat
     3541 tatctttctt tgttcaccta ataatccaac cggcaatttg ttgaacagtg acgatataaa
     3601 atttattctc gaaaatttta aaggcatcgt ggtagtagat gaagcctatc aggattttgc
     3661 aggaaatgtg tcatggctgc acagcttaca ggattatccc aatttagtgg tcatcaatac
     3721 attttcaaaa gcatggggca tggcagatat ccgcttgggt atgctgttcg caaatgaaga
     3781 aatcatccgt tatatgaata cgataaaaat gccttataat atttcacaac acaccattaa
     3841 tacggcaata gaagcattac ataaaacaat taaaaaacat gctttcagct acctttttat
     3901 ctttattatc cttgttgtct ttgttatcct ttttatcctt attgtct
//
LOCUS       DAOQII010000242         3924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14180_length_3924_cov_5.760662, whole genome shotgun sequence.
ACCESSION   DAOQII010000242 DAOQII010000000
VERSION     DAOQII010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14180_length_3924_cov_5.760662"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..918
                     /locus_tag="PLF48_10170"
     CDS             88..918
                     /locus_tag="PLF48_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNF49735.1"
                     /translation="MLLQQLPTQAGFDFLAKIKDMAISYAPKLIGAILVYLIGSFLIS
                     RLVSVLRKIMDKKQYDKSLQGFLVSLVKVLLTVLLLLSIAGMIGIDITSFAALLAGAG
                     LALGTALNGSLGNFAGGVMLLIFKPFKVGDLIEAQSVAGTVTEMGVFATTILTADNKT
                     VIIPNGPLSTGIITNYTTHGNLRVDLNMAIAPSQDIDKARKVAIETMLTHPKVLKNPA
                     PSVNVAKVADGMCTLVLQPYTVQADYWDVYFGVQEIVKKAWDAHNIEGPTPTRLIINK
                     "
     gene            complement(972..1331)
                     /locus_tag="PLF48_10175"
     CDS             complement(972..1331)
                     /locus_tag="PLF48_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49736.1"
                     /translation="MKFGCFILIFLLSFAHVSKAGNDKLVANIADEICKCLTSTHDSI
                     IKNTEYTTCVVEKVSPFKKAIMKILRKSMKEAMNEDSFSDQEIENFNKKLDAALYKTC
                     PNKAKAFIDIVDLLSDE"
     gene            1431..1853
                     /locus_tag="PLF48_10180"
     CDS             1431..1853
                     /locus_tag="PLF48_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49737.1"
                     /translation="MQTYGSLKHQVKSINELIFGCGFYYYWADEHKRAMRENSSANKN
                     GKTLVMYAMFDVCYPHTIELYFKKCTEHNLPKDYFIPDHWHIPPLELESIDFETQKET
                     QDDNLIRQFTMRLYTSSSFFEEPFFRIKAKSFYFEKVK"
     gene            1952..2596
                     /locus_tag="PLF48_10185"
     CDS             1952..2596
                     /locus_tag="PLF48_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49738.1"
                     /translation="MDKIFTISCFYFLCYLFVLSGKAKANTFAVLAKADSFFQADYYK
                     QALAIYESVPVADYSTKQLSNRTQCELSLGKYDAAETNSSILLQTDSFNAKLYIMRAK
                     SLLFQNLKHPQYDCIIADYKKAISLQHNADDYLKLLQQQIAALQQFIQDYKLKKLDWT
                     NAITQNILYCKDFLENSNFAKQQFKALIPKIEVLKNAYMNLLNPKPLPTNTTHE"
     gene            2589..>3924
                     /locus_tag="PLF48_10190"
     CDS             2589..>3924
                     /locus_tag="PLF48_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HNF49739.1"
                     /translation="MNSKQTHKIRFLFILLILQVLPLFAQVYNNNPCSLTYPICADGL
                     TNTITIASTDNNDNTAPTPASCTGGSGGNGGYVTTPTAGGDVWVYYQVQNPVSTADTL
                     VIETFAGTLTDIACAVYKVSNPCNFATYTQIACSRDKSGTNYMTKVALTSGVVPGDIL
                     LIRMWDESENQLGNFNLVVRTTNTLDEMDELNGLTVYASPTCYRDLFDSGGPYVDATN
                     QGFYANNENYTVTYCGEKTNDHVYFYTNFIDPAIFPTNTIYQQLLLGTTTTGNDYLTF
                     YDGPNTSSPQIGAYTGNTPNYPQPGTIISTGRCLTINFKSDPTLNTYGFSASILSISD
                     NVLNDSYNTYNVTCGSSLVYTDNGGTGANYQDFSNDVKIYCPSDNSKAIWASFDPNSE
                     LETNADYLYVYDGNSTSAPLINAYTGGFTGTGSNLANFSPNDLNSLGIIKATT"
BASE COUNT         1279 a          745 c          688 g         1212 t
ORIGIN      
        1 tatttataat atttaaattt aaaaaaaata ctgaaaacga cttaatcggc ataaattttg
       61 ttgaaaatag acaaaaaaaa ttaccttatg cttttacaac aacttcccac acaagccggt
      121 tttgattttt tagctaaaat caaagatatg gctatttcgt atgcacccaa actgattggc
      181 gccatactgg tttatctgat tggttctttt cttattagtc gccttgtcag tgtattacga
      241 aaaatcatgg acaaaaaaca atatgacaaa tcgttacagg gctttttggt ttctctggtg
      301 aaggtattgc ttactgtctt gttgttattg agcatcgcgg gtatgatcgg aatagatatt
      361 acctcttttg cggcacttct tgccggcgcc ggcttggcat tgggcacggc gctaaatggt
      421 tctttgggca attttgctgg tggagtaatg ctgctgatat ttaagccttt taaagtcggt
      481 gatttaatag aggctcagtc cgtagcaggt acggtaactg aaatgggcgt attcgcaact
      541 acaatattaa cggcagacaa caagacagta attataccaa acgggccctt gtccacaggt
      601 attattacca attatactac ccacggcaat ttgcgtgtag atttgaatat ggcaattgca
      661 ccttcacagg atatagacaa ggcgcgtaaa gttgccattg agaccatgct aactcatcct
      721 aaagttttaa agaatcctgc gcctagtgtc aatgttgcaa aagtagcgga tggtatgtgt
      781 acactggttt tacaacctta caccgtgcag gcagattact gggatgttta ctttggtgta
      841 caggaaattg taaaaaaagc atgggatgct cacaatatag aaggccctac gcctacccga
      901 cttatcataa acaaataact ataaatgaaa taaaatttat aatcagccgc atgtaaaagt
      961 gcggcttttt tttattcatc agaaagtaaa tcaacgatat ctataaatgc ttttgctttg
     1021 ttgggacaag ttttatataa ggcagcatcc agtttcttgt taaagttttc tatctcctgg
     1081 tcagaaaaag aatcttcatt cattgcctct ttcatggatt tgcgtagaat tttcatgata
     1141 gcttttttaa agggagatac cttctcgact acacatgttg tatattccgt gtttttaatt
     1201 atactgtcgt gtgttgaagt gagacactta cagatttcat cggcaatatt tgcaactaat
     1261 ttatcatttc ctgcttttga aacgtgagca aatgataata gaaatatcaa aataaaacaa
     1321 ccaaacttca taaactaaaa ctaacatttt ttttaaaggt tgagataccc tgaattgcgt
     1381 aatttgactt gccaacttta gctaagtttt ttagatttga ataaaatttt gtgcagactt
     1441 atggttcact aaaacaccag gtaaaaagta tcaatgaact tatctttggt tgcgggtttt
     1501 attattactg ggctgatgaa cataaacgag ctatgcgcga aaatagcagc gcaaacaaaa
     1561 acgggaaaac gctggtaatg tatgctatgt tcgatgtttg ttatcctcat acgattgaac
     1621 tctattttaa aaaatgtacg gaacataatt tgcctaaaga ctattttatt cctgatcact
     1681 ggcatattcc acctttggaa ctagaaagta ttgatttcga aacacaaaaa gaaacacaag
     1741 acgataattt gattaggcaa tttacgatgc gactatacac gagttcttcg ttctttgaag
     1801 aacctttctt tcgaataaaa gccaagtcgt tttattttga gaaagtaaaa taggagggta
     1861 aaaccctcaa acaaaataat gtgccataat ataaatattg aataagtatt actatcttta
     1921 ttccagtcta ttatgaatct aataaaaata catggataaa atttttacaa tttcctgttt
     1981 ttatttttta tgttatttat ttgttctgtc tggcaaagca aaagcaaata cctttgctgt
     2041 cttagccaaa gcagattcat tctttcaagc cgattattat aaacaagcct tggctattta
     2101 tgaatcggtt cctgttgctg actattcaac aaagcaatta agtaaccgca cacagtgtga
     2161 gctcagcctt ggaaaatatg atgcagcaga aacaaacagt tctattttat tacaaacaga
     2221 ttcttttaat gctaagcttt atataatgcg tgcaaaaagc ctgctcttcc aaaacctgaa
     2281 acatccacag tatgattgca tcatagcaga ttataaaaaa gccatttctc tccaacacaa
     2341 tgccgatgac tatttgaaat tattacaaca gcaaatagca gcattacagc aatttattca
     2401 agattataaa ctgaaaaaat tagactggac aaatgcaatt actcaaaata tactctattg
     2461 taaggatttt ttggaaaaca gcaacttcgc aaaacagcaa ttcaaagcac tgattccaaa
     2521 aattgaagtg ctgaaaaatg cctatatgaa tttattaaac cccaaaccac tcccgaccaa
     2581 taccacgcat gaatagtaag caaacgcata aaattcgctt cctctttatc ttgcttattt
     2641 tgcaggtctt gccattgttt gcccaagtat ataacaataa tccatgcagt ctgacatatc
     2701 ccatttgcgc agatggttta accaatacaa ttaccattgc ctccacggat aataatgaca
     2761 atacagcacc aactcccgca tcttgtactg gaggctcggg tggtaatggc ggttatgtaa
     2821 caacaccgac tgctggtggt gatgtatggg tttattacca agtacaaaat ccggtatcaa
     2881 cggcagatac tttagtgata gaaacatttg ccggcacttt aacggatatt gcttgcgctg
     2941 tttataaagt aagtaaccct tgcaattttg caacatacac gcagatagca tgcagccgcg
     3001 ataaatcagg aacaaactac atgacaaaag tggcactgac ttccggtgtt gtacccggtg
     3061 atattttgct gatacgcatg tgggatgaaa gtgaaaacca attgggaaat tttaatttgg
     3121 ttgtcagaac aacaaataca ctggatgaaa tggatgaact caacggtctg accgtatatg
     3181 cttctccaac gtgctaccgg gatttatttg acagcggcgg tccttatgta gatgcaacca
     3241 accagggatt ttatgccaat aatgaaaatt atactgttac ctattgtggc gaaaaaacaa
     3301 atgaccacgt ctatttttat accaacttta ttgatcctgc tatttttcca acaaatacca
     3361 tctatcagca attattatta ggaacgacta caaccggaaa tgattatctg actttttatg
     3421 atggtccgaa tacttcatct ccgcaaatag gtgcttatac aggaaacacg cccaactatc
     3481 cgcagcccgg cacgattatt tccaccggaa gatgtcttac gatcaacttt aaatctgacc
     3541 caacactgaa tacatacgga ttttcggcat ccattttatc aatatcggat aatgtgttga
     3601 atgattctta taacacttac aatgtaacct gtggtagctc cctagtctat acagacaacg
     3661 gaggaacggg tgctaattac caagattttt ctaatgatgt aaaaatatat tgcccttctg
     3721 ataattcaaa agccatttgg gcatcttttg accccaattc agaactggaa accaatgccg
     3781 attatttata tgtttatgat ggaaacagca ccagtgcacc tttaattaat gcatataccg
     3841 gcgggtttac aggtaccggc tccaacttgg caaatttttc tccaaacgat ctaaactctt
     3901 taggcattat aaaagcaacc acaa
//
LOCUS       DAOQII010000243         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14205_length_3919_cov_4.796843, whole genome shotgun sequence.
ACCESSION   DAOQII010000243 DAOQII010000000
VERSION     DAOQII010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14205_length_3919_cov_4.796843"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLF48_10195"
     CDS             <1..247
                     /locus_tag="PLF48_10195"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF49740.1"
                     /translation="TPGSPVAETCNGIDDNCNGLVDESLTRSCYSGPSGTAGVGKCRA
                     GTQTCASGAWGLCIGEVLPSPEIIGNGIDDNCDGIVQ"
     gene            complement(407..1177)
                     /locus_tag="PLF48_10200"
     CDS             complement(407..1177)
                     /locus_tag="PLF48_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF49741.1"
                     /translation="MINSIIVEDEPIFQEVLHKAIKASGLPVKVCGICNSKRDAKKLL
                     AEHKPQLVFLDVELADGKGIDLLAELNPVEFETIFTTSHDKYAINAIKNNAADYLLKP
                     IKEKDLKTALLKVQTRLNEKESLKQIAQVQTYLENLKTKEQQDYKLMVTTKEGVHLLK
                     VNDIIRLESESNYSLVYLVNHKKYMVSKTLKYFEEKLLPYNFMRIHQSHLINLLHMKE
                     IDHADNICVMSDGAKLEISKRKKKDFMDSVSGSKTTIA"
     gene            1218..3323
                     /locus_tag="PLF48_10205"
     CDS             1218..3323
                     /locus_tag="PLF48_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF49742.1"
                     /translation="MFRFIQQSIVKIKMTAHSKKISLPDLTALTEKAISLREQKKYLK
                     SLQLFNKVLDAENLDKDSIACATMHKETGDTYYELKEYAKAFEHHSIALDLYLQNKEL
                     DNYLEQLKRMGTMQQAVWQYPKAQELFQQGLNTAVRMGSMKSIVEFEILLGNCFNWAD
                     KFEEAERYLKSAIQKEQHIRMPVFEIRAKVSYAILLRKMKKYDEAEKYFKIGYKLSKE
                     NGNIMFMDITKSYGIMHYELGNYDTALSLLSESIKSKEIREKPIVKAVINEYLSLVYE
                     TKGDYKNALKHARDFYEARMTLIEQGFSDENNKLQAKIGLNDARREQHIAEETAQAKS
                     LFIATISHEIRTPMNIILSTTALMQNDHPKPEHIKYLDTLRRSGENLLGIINDILDIS
                     KIEAGKLEIEYEPVVLQDVFENIYQTMLQPAKDKNLKLFYTLDNKLTFPIYSDALRLT
                     QIITNLISNSIKFTAKGEIHFQAKIKNKNTLFLQIHDTGIGIPKDKLPTIFDQYEQVR
                     TQVQKKYKGTGLGLAICKKLVEMMNGQISIKSKVNEGTCFCIELPFQKAKIQQTKAST
                     IQPFEDTSFLADKHILIADDFEDNRFVFKETLRLFNPTVRISEAENGSLALDFCKTTS
                     DIDLIIMDLDMPEMNGFEALTEIRKLKKHKKTKVIASTASLVSNQEDEFIAFGYSGFL
                     PKPFTLEILYNILRKQFNA"
     gene            complement(3345..>3919)
                     /locus_tag="PLF48_10210"
     CDS             complement(3345..>3919)
                     /locus_tag="PLF48_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNF49743.1"
                     /translation="SSTALVVTAGIAEIAAGSIAMGLGGYLAGQTEIDHYGTEEKREY
                     YEVDNLREKEIGETEDIFRSMGLSENTIKYAVHELAEDKDKWVKFMMKYELGLEEPDP
                     KRARKSAFTIGISYIIGGLVPLVPYFFFEDPKQGLVYSAIITLISLFVFGYFKSKLTG
                     QPLFKGALKVTAIGAVAAGTAYLVAKLIGG"
BASE COUNT         1283 a          748 c          708 g         1180 t
ORIGIN      
        1 tacgcctgga agtcctgtag cagaaacttg taatgggatt gatgataatt gtaatggtct
       61 agttgatgag agccttacca gatcttgtta ttcagggcct tcaggtactg ccggagtggg
      121 taaatgcaga gcaggcaccc aaacttgtgc ttcaggagct tggggcttat gtattggaga
      181 agtgctacct tctccggaaa ttattggtaa tggaattgat gataattgtg atggaatagt
      241 ccaatagcgt ctatttaaac agcctcttag tagtgagtgg tttagcagct ttattaaaat
      301 aattcattca ctttcaagaa tcccatcttg actcgacttt ctttttaaag tgatgagatt
      361 gtgtaaaacc aattctctta ttattgttct tgtcttgtaa aattgcttac gcaatagttg
      421 tcttactgcc acttaccgaa tccataaaat ctttcttttt acgtttggat atttcgagtt
      481 tagccccatc actcatcaca caaatattat cagcatggtc tatttctttc atgtgtaata
      541 aatttatcaa atgcgattga tgaatgcgca taaaattgta gggcaacaac ttctcttcaa
      601 aatactttaa tgttttggac accatatatt tcttatgatt taccagatag actaaggaat
      661 aattactctc cgattccagt ctgataatat catttacctt taacagatgt acgccttctt
      721 tggtcgttac cattaattta taatcctgct gttctttagt ctttagattt tctaaatacg
      781 tctgtacttg tgcaatttgt ttcagagatt ccttttcatt caaacgggtt tgtactttca
      841 gtaatgcagt ttttaaatct ttctctttaa taggttttaa taagtaatca gcagcattat
      901 tttttatggc attaattgca tatttatcat gagaggtggt aaatatggtt tcaaattcaa
      961 cgggattaag ctccgcaagc aaatcaatgc ctttcccgtc ggctaattct acatctaaaa
     1021 aaacaagttg tggcttatgt tcagctagca gctttttggc atcgcgtttg gaattacaaa
     1081 ttccgcaaac cttcaccgga agaccactgg ctttgatagc cttatgcaag acttcctgaa
     1141 agatgggttc atcttccaca attatagaat ttatcatagt tagtttcgta tttagtagca
     1201 tataaattta agaataattg tttagattta tacagcaatc tattgtaaaa attaagatga
     1261 ctgcccactc taagaaaata agcttaccgg acttaactgc attaacggaa aaagccatca
     1321 gcctccgcga gcaaaaaaaa tatctgaaat ctttacaact gtttaataaa gtactggatg
     1381 ctgaaaattt agacaaagac agcatcgctt gtgccactat gcataaagaa actggcgaca
     1441 cctattacga actgaaagaa tatgctaagg catttgagca ccactccatt gcgcttgact
     1501 tatatttgca aaacaaagaa ttggacaact atttggagca actgaagcgt atgggtacca
     1561 tgcaacaggc agtttggcag tacccaaaag ctcaggagtt gtttcagcaa gggctgaata
     1621 ctgctgtcag aatgggcagt atgaagagca tagttgagtt tgagatttta ttgggtaatt
     1681 gctttaattg ggcagataag ttcgaagaag ccgaacgcta tttaaaaagt gccatacaga
     1741 aagaacaaca tatccggatg cctgtatttg aaattcgggc aaaagtaagt tatgccattt
     1801 tgcttcgcaa gatgaaaaaa tatgacgaag cagagaaata ctttaaaata gggtataaac
     1861 tatccaaaga aaatggcaat atcatgttta tggatattac caaaagctat ggtattatgc
     1921 attacgagtt aggcaattac gacaccgcgc tttctttact ttcagaatca ataaaatcaa
     1981 aagaaatcag ggaaaaacct atagtaaagg cggttatcaa cgaatatctt tctctggttt
     2041 acgaaacaaa aggcgattac aagaatgcac ttaagcacgc tcgcgatttc tatgaagcac
     2101 gcatgacctt gattgaacaa ggattttcag acgaaaacaa caaactgcaa gcaaaaatcg
     2161 ggttgaatga tgccagaaga gaacagcata ttgccgaaga aactgcacaa gctaaaagtt
     2221 tgtttattgc taccatctcg cacgagatac gcacaccgat gaatattatt ttatcgacaa
     2281 ctgctctgat gcaaaacgac caccctaaac cggaacatat caaatacctg gacacgctaa
     2341 gacgcagtgg agaaaatttg ctgggtatca tcaacgatat tttagatatc tcgaaaatag
     2401 aggccggaaa gttagaaata gaatatgaac ctgttgtgct gcaggatgtt ttcgagaata
     2461 tatatcaaac gatgttgcag cctgctaaag acaaaaacct aaaactattc tacacgttag
     2521 acaataagct gacatttccc atttattcag atgcactccg acttacccaa atcattacta
     2581 atctgatttc caattccata aaatttacgg ctaaaggaga gattcatttt caggcaaaga
     2641 taaaaaacaa gaacacgctc tttctacaga ttcatgacac cggaattggc atacctaaag
     2701 ataaactgcc aaccatcttt gatcaatacg agcaagtacg aacgcaagta caaaagaaat
     2761 ataaaggaac aggtttgggc ttggctattt gtaagaaact ggtagaaatg atgaatggac
     2821 aaatcagcat aaaaagcaaa gtaaatgagg gtacatgttt ttgtattgag ttaccatttc
     2881 aaaaggctaa gattcaacaa actaaggcaa gtacaataca gccctttgag gatacttcat
     2941 tcttagccga caaacacatt cttattgccg atgactttga agacaaccgt tttgtgttta
     3001 aagaaacgct gcggctattt aatcctacag tccgcatcag cgaagcagaa aatggcagtc
     3061 tggcattgga tttctgtaag acaacttcag atatagacct cattattatg gatcttgaca
     3121 tgcctgaaat gaatggattt gaagcactta ccgaaattcg caaacttaaa aaacacaaaa
     3181 aaaccaaagt cattgccagc acggcaagct tagtcagcaa tcaggaagat gaatttattg
     3241 ccttcggtta ttccggtttt ttaccaaaac cttttacctt agaaattttg tacaatatac
     3301 ttaggaaaca atttaatgct taagttttac atcaatatta agtcttatcc acctatcaat
     3361 ttcgcaacta aataagctgt ccctgctgct accgcaccaa tagctgttac ttttaatgct
     3421 cctttgaata gcggctgtcc ggttagtttt gacttaaaat aaccaaaaac aaacaagctg
     3481 attaaggtga taatggcaga atagaccaac ccttgtttgg ggtcttcaaa aaagaaataa
     3541 ggaacgaggg gcactaatcc gcctatgata taagatatgc ctatagtaaa tgccgatttt
     3601 ctggcacgtt tcggatccgg ttcttccaac cccaattcat atttcatcat aaactttacc
     3661 cacttgtctt tatcttcagc taattcatgc acggcatatt ttatagtgtt ctcactcaag
     3721 cccatggagc ggaagatatc ttcggtttct ccaatttctt tttctctaag attatcaact
     3781 tcataatatt cccgcttttc ttcggtgcca taatggtcta tttcagtttg tcctgccaaa
     3841 taaccgccta aacccatcgc aatagatcct gcagcaattt ctgctattcc ggcagtaacc
     3901 accagtgcag ttgatgaaa
//
LOCUS       DAOQII010000244         3916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14221_length_3916_cov_4.391867, whole genome shotgun sequence.
ACCESSION   DAOQII010000244 DAOQII010000000
VERSION     DAOQII010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14221_length_3916_cov_4.391867"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLF48_10215"
     CDS             complement(<1..474)
                     /locus_tag="PLF48_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HNF49744.1"
                     /translation="MKKTVFIVSCAIVVLMASCNTGSNGNVHAGKIKKDVDSASYTIG
                     VVLGKNLRNGEVDSTFNLDLIYKGIYDELNSKKLAIPFDQSTSLMQVQRFVADRNLVS
                     GKKFLDEMAKKSGVKKTASGLEYEVIKEGTGEKPVESSIVKVHYKGTFPDGTEFDN"
     gene            complement(500..1312)
                     /locus_tag="PLF48_10220"
     CDS             complement(500..1312)
                     /locus_tag="PLF48_10220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNF49745.1"
                     /translation="MKHYYLFLLSFVCLSTASFAQKTTTVKPKTTKAPTKPVTVKPPI
                     NPFKTKFDSISYFIGIQIGNDMKNNGATELQPSQISKGIKDAMSGTKPQLDMQATMML
                     AQNYFMQKQQEKAMMKMLQNQQFFAQNATRPGVKTTASGLQYEILKDTAGPKPLATDM
                     VKVHYHGTFLDGKVFDSSVERGQPAQFPLNGVIPGWTEGLQLMSVGSKYRLYVPAKLA
                     YGEQGMPEGGIGPNEPLIFDVQLLDIVKQQPQQQPDIQVVPQTPTNLPSGGQ"
     gene            complement(1417..2544)
                     /locus_tag="PLF48_10225"
     CDS             complement(1417..2544)
                     /locus_tag="PLF48_10225"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNF49746.1"
                     /translation="MSTRISFLKVFTVVIVALLSILVLSLNHTLRVPSIFDGKLYTQL
                     EIKKAQKPFYIPEAAIDLPPLSNWADSSIAQQYGYIDLLLKNSNTTSFLVLRNDTLIF
                     KRYLNGVKEGDVTQLFSVTKVFVTAMAGIAVKEKYFESVEDPVTKYLKKLQEQEFNKL
                     TLLQLMQMQSGLNYDEYGQWYKTLAFYYEPNLTNAIQHAKFIKQPGTEYKYKSIDTQI
                     LGECINAAIGKHKKITDYFYDEIWNKIGAQDSASWSLDSKIHRSPKYYGGLNMSARDL
                     AKFGMMVAHNGKFKNKQVLPKNWFHYCDNTLHRSSEEEKYCMGWYYAHDDDSSNVYYA
                     AGFGGQIMLINETKNVIVIRLGTDRGGVSWYSMMKKLSEII"
     gene            complement(2544..3293)
                     /locus_tag="PLF48_10230"
     CDS             complement(2544..3293)
                     /locus_tag="PLF48_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49747.1"
                     /translation="MRFFLGILLVFAFQVVMAQDSSLVVQQTSKPKKIKYDFSRVHFM
                     IGMDYGYISPIITERDRFNDSYYPVLDSAPVWVNRNTTTNFYTTNKIGNIQFWLSGNF
                     WNNLYIGMSYQFFTIKQYKKDKNMGNLLSTRSSMFFLVSARFSYVFELLKNKCLQIEP
                     ALKIGGYTADDYYDRGLGKKFYMGADVRIRYLIKKKFGFSLGMDYDYIRYKRSGTNAL
                     FQQDYNQKTTFNNLHFNVGICGNITINTRKK"
     gene            complement(3353..>3916)
                     /locus_tag="PLF48_10235"
     CDS             complement(3353..>3916)
                     /locus_tag="PLF48_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49748.1"
                     /translation="LPVELTSFTGYNDGQINVLDWITASEINTSKFEVEKSTDGYSFS
                     YLGERPAAGNSTMPRTYQLVDNNPVIGNNYYRLKMIDIDGTYDYSDKILIKVADLQTN
                     EGIVNIYPNPTKGEVNIVYQAAADHSAMLTIFNTIGQLMTDNSYQMKAGVNTITISAT
                     DYAKGLYIINLQNTDAGIKYQSKFVKE"
BASE COUNT         1114 a          772 c          659 g         1371 t
ORIGIN      
        1 gttatcaaat tcagttccat ctgggaaagt gcctttgtaa tgtactttca cgatactgct
       61 ttccaccggt ttttcgccag taccttcttt tattacttca tattccaagc cactggctgt
      121 ttttttaacc cctgatttct tagccatttc atccaaaaat ttctttccgc ttaccagatt
      181 tctgtcagct acaaaacgct gtacttgcat taagctagta ctttggtcaa aaggaattgc
      241 taattttttg ctgttcagtt cgtcataaat tcctttataa atcaggtcaa gattaaaagt
      301 actatccact tcgccattgc gcaggttttt tcctaaaacc acaccaatag tgtatgacgc
      361 agagtctaca tcttttttaa tttttccggc gtgtacattt ccattactac cggtgttgca
      421 tgatgccatc aatacgacga tggcacacga tacgataaat actgtttttt tcattgattt
      481 attaataata tttaaaaatt tattgtcctc cacttggtaa attagtcggt gtttgcggca
      541 caacctgaat atcgggttgc tgttgtggtt gttgttttac aatatcaagt aattgcacat
      601 caaaaatcaa aggttcgttg ggtccaattc caccttcggg catgccttgt tcaccatagg
      661 ctaatttagc aggcacatac agtctgtatt ttgaaccgac agacattaac tgcaatcctt
      721 cagtccaacc cgggataaca ccgtttaacg ggaattgtgc aggttgtcct ctttctacag
      781 agctgtcaaa tactttgccg tctaaaaatg ttccgtgata atgcactttt accatatcag
      841 tagcaagcgg tttaggccct gctgtatctt tcagtatttc atattgcaag ccactggcag
      901 tcgtttttac tccgggacgg gttgcgtttt gtgcaaagaa ctgctgattt tgcaacattt
      961 tcatcattgc tttttcttgc tgtttttgca tgaaataatt ttgcgccaac atcatcgtag
     1021 cctgcatgtc taattgaggt tttgtaccgg acatggcatc ttttatccct ttgctgattt
     1081 gcgaaggttg caattctgtc gctccgttat ttttcatatc gtttccaatc tgtatgccta
     1141 taaaatagga aatagaatcg aattttgttt taaacggatt aattggaggc tttaccgtca
     1201 ccggctttgt gggtgccttt gtagttttag gctttaccgt cgtcgttttt tgagcaaatg
     1261 atgcagtgct caagcaaacg aaagaaagaa gaaataagta gtaatgtttc attttttcaa
     1321 ttgagccaca aaaataaggg tttcctatgg attaatccgg taattaacta tttttaaaac
     1381 aatatttatg ttctgtagtc agtattgtgc gcaatttcaa atgatttctg aaagtttttt
     1441 catcatagag taccaggaaa ctcctcccct gtcggtgccc agccggatga caatcacatt
     1501 tttcgtttca tttatcaaca taatctgccc tccgaaaccg gctgcataat agacatttga
     1561 actatcatcg tcatgtgcat aataccaacc catgcaatat ttttcttctt cactgcttcg
     1621 gtgtaaagta ttatcacagt aatgaaacca atttttaggc agtacttgtt tatttttgaa
     1681 tttaccgttg tgcgcaacca tcatcccgaa tttagccaaa tcacgtgccg acatattcaa
     1741 acctccataa tattttgggg agcgatgaat tttgctatcc aaactccacg atgcggaatc
     1801 ttgagcgcct attttattcc agatttcatc gtaaaagtaa tccgtgattt ttttgtgttt
     1861 gccaattgct gcattgatac attcacctaa aatctgcgta tcgatggatt tgtatttgta
     1921 ttccgtaccg ggctgcttta taaatttggc gtgttgtatg gcattggtca gattaggttc
     1981 ataataaaat gccagtgttt tataccattg tccatattca tcataattta agcccgattg
     2041 catctgcatc agttgtaaaa gtgtcaattt attaaattct tgctcctgca gttttttcaa
     2101 atattttgta accggatctt caacactttc aaaatacttt tctttcactg caataccagc
     2161 catggctgtt acaaatactt tcgttacaga aaaaagctgg gtaacatccc cttcttttac
     2221 accgttaagg tatcgtttaa aaatgagggt atcattgcga agcacaagaa aagaagtggt
     2281 attgctattt ttcagcaata agtcaatata gccgtattgc tgtgctatgc tgctgtcagc
     2341 ccaattactc aacggtggta aatcaatggc tgcttccgga atataaaatg gtttttgtgc
     2401 ttttttaatt tctaactgtg tatataattt gccatcaaat atagatggta cgcgtaaagt
     2461 atgatttaat gaaagaacta atatactcag cagtgcaaca atgacaactg taaatacttt
     2521 aagaaacgaa atccgtgttg acattatttt ttacgtgtat ttatcgtaat atttccgcaa
     2581 attcccacgt taaaatgcaa attattaaat gtggttttct ggttataatc ttgttgaaat
     2641 aatgcatttg taccactgcg tttataacgt atatagtcat aatccatacc cagactaaag
     2701 ccaaatttct tttttatcag ataacgtatt cttacatctg cacccatata aaatttcttc
     2761 cccaaacctc tgtcataata atcgtcggcc gtatatcctc cgattttcaa agcaggttca
     2821 atttgcaggc atttattttt cagcaattcg aaaacataag aaaatctggc agatacaaga
     2881 aaaaacatgg agcttctggt tgacaacaga tttcccatat tcttatcttt cttgtattgt
     2941 ttaatcgtaa aaaactgata actcattccg atatacaggt tattccagaa attaccggac
     3001 agccaaaact gaatattacc aattttgttt gtcgtataga aattggtagt ggtatttctg
     3061 tttacccata caggagctga atccaaaacg ggataatagc tgtcattaaa acgatctctt
     3121 tcggtaataa tcggtgagat atatccgtaa tccattccta tcatgaagtg cactctggaa
     3181 aaatcgtatt ttattttttt aggtttgctt gtctgctgta caactaaaga agaatcttgt
     3241 gccatgacaa cttgaaaagc aaaaacaagt aatattccta aaaaaaatct cattgggata
     3301 aaaataaaaa atcccgacga ataagtcggg atttttttct gtttcaggct tattattctt
     3361 tcacaaattt cgactggtat ttaatgccgg catctgtgtt ttgcaaattt ataatataca
     3421 atccttttgc gtaatcggtt gcagaaatgg taatggtatt gactcctgct ttcatttgat
     3481 agctattatc cgtcatcaac tgaccgatag tattaaatat cgtcaacata gcactatgat
     3541 cagcagcagc ctgatatact atatttactt ctccttttgt cggatttgga taaatattta
     3601 caataccttc attggtttgc aagtcagcca cttttatcaa tattttatcg gaatagtcat
     3661 acgtgccgtc aatatctatc atttttagtc tgtaataatt attcccgatt accggattat
     3721 tatcaacgag ttgatacgta cgtggcatgg tgctgttacc cgcggcaggt ctttctccca
     3781 agtagctaaa actataacca tctgtcgact tctctacttc aaatttagac gtatttattt
     3841 cagatgccgt tatccaatct aacacattta tctgaccatc attatagccc gtgaaggacg
     3901 tgagctctac cggcaa
//
LOCUS       DAOQII010000245         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14456_length_3880_cov_4.774379, whole genome shotgun sequence.
ACCESSION   DAOQII010000245 DAOQII010000000
VERSION     DAOQII010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14456_length_3880_cov_4.774379"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLF48_10240"
     CDS             complement(<1..816)
                     /locus_tag="PLF48_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNF49749.1"
                     /translation="MEAIKGNIKTEYALYPKEVLAELEQSKKSLFLGIPKENDYQEKR
                     VPLTPNSVAALINYGFRVAIEKDAGTASHFSDEEYVRAGADVVYTKEDVYKADIIVKS
                     GPLSPNDITLLHPGQMIFSPILLPTQTKSMVKSMMEKRITAIAFEYVKGDYGTYPFMR
                     SMSEIAGNYAITIAARYLSNEYGKGILLGGIAGQPPARVVILGAGTVGESAAKSALAL
                     GASVQVFDDNIYRLSRLQNDLGLKIYTSVLDPINMAKNIERADVVIGAMKPKNG"
     gene            complement(833..1261)
                     /gene="tsaE"
                     /locus_tag="PLF48_10245"
     CDS             complement(833..1261)
                     /gene="tsaE"
                     /locus_tag="PLF48_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNF49750.1"
                     /translation="MIKLFEAVDLEGYDEVSDFILSFLPQTNTFCFYGDLGAGKTTLI
                     KTICSKLSVVDTVSSPTYPIINEYTTKNGDTIYHLDLYRLQSINEARQAGVEEVLYSD
                     KTCLIEWPDNFEEILPIPHLKINIGKLDDGKRSVETILTT"
     gene            complement(1312..2868)
                     /locus_tag="PLF48_10250"
     CDS             complement(1312..2868)
                     /locus_tag="PLF48_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNF49751.1"
                     /translation="MSEIKILWADDEIELLKPQILFLEEKGYIVDPVSNGHDALDKFK
                     SEHYDLIFLDEHMPGISGLETLSRIKTISKDTPIVMITKNEEENIMEEAIGSQIADYL
                     IKPVRPNQILMSVKKLTENKKLVSEKTMVNYRQEFQNIMLELNNVNTHEEWAELYKKL
                     IYWELELDHANNTDMKGILDMQKSEANKEFFKFISKNYLKWIKDLNNTATPTLSATLI
                     KNKLLPHLQQDTTTFFILIDNLRYDHFRVIESLIKQDFKKVSEEHFFSILPTTTQYSR
                     NAIFSGLMPLEIDKKYPDRWFYDDENMGKNLSEEFFLNEQLQRLGFTGKTSYTKITTN
                     KDGKSLEDNMLNLMHNSLNVIVYNFVDMLSHARTEMEVLKELAGDESAYRSLTLSWFD
                     HSPLLNAIQKIKDKKVRLIFTSDHGTIRVQNPSKIIGDKATTTNLRYKTGKNLNYNEK
                     EVLAFRNPEEAMLPKSNVSSSYVFAKEDYYFCYPNNYNHFVNYYRNTFQHGGVSLEEI
                     IVPVAVYESK"
     gene            3272..>3880
                     /locus_tag="PLF48_10255"
     CDS             3272..>3880
                     /locus_tag="PLF48_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF49752.1"
                     /translation="MNKRKIFNDPVYGFITIPYEIVFDVIEHPYFQRLRRIQQLGMSS
                     MVYPGATHTRFHHSLGAMHLMHRSLEVLKSKGIQITEQESQAAILAILLHDLGHGPFS
                     HALEGILLKNYHHEDISILLMDKLKMIATEPIEMAIAIFKNTYPKKFLHQLVSGQLDM
                     DRMDYLNRDSFYTGVSEGVIGYDRIINMLHVHHDNIVVEEKGI"
BASE COUNT         1144 a          726 c          692 g         1318 t
ORIGIN      
        1 gccgttttta ggtttcattg cgccaataac gacatcggct ctttcaatat ttttggccat
       61 attaattggg tctaaaacgg acgtgtaaat ttttaacccc aaatcatttt gtaaacgcga
      121 cagtctataa atattatcat caaatacctg cacactcgcc cctaacgcca aggcactttt
      181 tgctgcagat tcgccgactg tacccgctcc gagtataact accctggcag gaggctgacc
      241 tgcaatacca cccaacaaaa ttccttttcc atattcgttg ctcaaataac gggctgctat
      301 agtaatggca taattccctg ctatttcact catgctgcgc atgaacgggt aagtaccata
      361 gtcgcctttt acatattcga aagcaatagc cgtgatgcgt ttttccatca tgctttttac
      421 catgcttttg gtttgcgttg gcaataaaat aggagagaaa atcatttgcc cgggatgcaa
      481 gagcgtgatg tcgttcggac ttaaaggacc ggatttcacg atgatatctg ccttatatac
      541 atcttcttta gtatacacta catctgcacc ggctcgtaca tattcttcat cagaaaaatg
      601 gctggcagtt ccggcatctt tttcaatagc cacccgaaat ccataattta taagtgccgc
      661 tacagagttt ggtgtaagtg gcactcgttt ttcttgataa tcattttctt taggtattcc
      721 taaaaacaag gattttttag actgttctaa ttctgcaagc acttctttcg gatagagtgc
      781 atattctgtt ttgatattgc cttttatagc ttccatgttg cccgcttttc gttcaggttg
      841 tcagtattgt ttctactgat ctttttccat cgtctaattt accaatattt attttaagat
      901 gtggtattgg aagtatttct tcaaaattat caggccactc tatcaggcaa gttttatcag
      961 agtataatac ttcttccacc ccggcctggc gagcttcgtt gatactttgc aagcgatata
     1021 aatcgaggtg ataaattgtg tcgccattct tggtcgtgta ttcgtttatt attggatatg
     1081 ttggagaaga aaccgtatca acaactgata atttgctgca tattgtttta attaatgttg
     1141 ttttccctgc acctaaatct ccataaaaac aaaatgtatt ggtttgaggt aaaaaagata
     1201 agataaaatc ggacacttca tcatatcctt ctaagtctac tgcttcaaaa agtttaatca
     1261 taaatcaaaa actgatttaa atagatttaa aataaacaaa taattatttt tctacttgga
     1321 ttcataaact gcaaccggaa cgataatttc ttctaaggaa acacctccgt gctgaaaagt
     1381 gtttctgtaa taattgacaa aatgattgta gttgttcggg taacagaaat agtaatcttc
     1441 tttggcaaaa acataagaag aactcacgtt agacttaggt aacatggctt cttccggatt
     1501 gcggaaggcc aatacttctt tctcgttata attcaggttt ttaccggttt tataacgcaa
     1561 gtttgttgta gtggctttgt cgccaattat ttttgaagga ttttgaacgc gtatcgtgcc
     1621 atggtcagag gtgaaaatca accgcacttt tttatctttt attttttgta tggcattcaa
     1681 taaaggagaa tgatcaaacc aggataaggt gagtgaacga taagccgatt cgtctccggc
     1741 taattctttc agaacttcca tttccgtgcg cgcatgcgat agcatatcta caaaattata
     1801 gacgattaca tttaaactgt tatgcattaa attgagcata ttgtcttcta atgacttgcc
     1861 atctttattc gtcgtgattt tagtataact cgtttttcct gtaaatccca ggcgttgcaa
     1921 ctgttcattt aagaagaatt cctcgctcag atttttgccc atattttcat cgtcataaaa
     1981 ccatctgtcg ggatattttt tatcaatttc gaggggcatt aatcccgaaa aaatagcatt
     2041 gcgagagtat tgggtagtgg taggtaaaat cgaaaaaaaa tgttcttcac ttactttttt
     2101 gaaatcttgt ttaatcagcg actcaattac acggaaatgg tcataacgca aattgtcaat
     2161 cagaataaaa aacgtagtgg tatcttgttg cagatgtggt aatagcttat tctttataag
     2221 tgtggcagac aaagttggcg ttgcagtgtt gtttaagtct tttatccatt tgaggtagtt
     2281 tttagaaata aacttgaaaa attccttgtt ggcttcactt ttttgcatat ctagtatgcc
     2341 tttcatatct gtgttgttgg cgtggtcaag ttcaagttcc caataaatca gttttttgta
     2401 taattcagcc cattcctcgt gtgtattcac gttattgagc tccaacatga tattttgaaa
     2461 ctcctgacga taatttacca tcgttttttc agaaaccagt tttttgtttt ctgtcagctt
     2521 ctttaccgac atcaggattt gattcgggcg taccggtttt atcaggtaat ctgctatttg
     2581 agaaccgata gcctcttcca taatattctc ttcttcattc tttgtaatca tcacgatggg
     2641 cgtatcctta cttatcgttt tgatacgaga taaagtttcc aacccggaga tgccgggcat
     2701 gtgttcatct aagaatatca aatcataatg ttcggattta aatttatcta aagcatcatg
     2761 tccgttgctt acgggatcaa cgatataacc tttttcttcc aaaaacagga tttgcggttt
     2821 tagcaattct atttcatcat ctgcccaaag tattttgatt tcgctcattt ttatataatt
     2881 attgaacttt atgtttattt aaaaatcatg cctttgtaat ttatttatta cctagctgat
     2941 ttccggacaa attaagtaat tttattcttt attaaatgtg aaattatcca aaaatgcaga
     3001 tttaatttta ctagaatata gaattaagag tcaagaacca agacgagagt aataggtaga
     3061 gagtattgag atatgagtag caagagccgg gaataaagac gagagtagta agtagagagt
     3121 attaagatat aagatttgag tattgagtag catgagaacc aatctctaat aaccaataac
     3181 caatccacca atctaccaat gcccaattac taataaccaa tctctaatga cctatcacta
     3241 atgactaatc accaaacacc aatcaccaac aatgaataaa cgcaaaatat ttaatgaccc
     3301 tgtttatggt tttataacta tcccctatga gattgttttt gatgttatcg aacaccctta
     3361 ttttcaacgg ctgcgcagaa ttcaacaatt agggatgagt tcgatggttt atccgggggc
     3421 aacgcatacc cgttttcatc attcactcgg tgccatgcat cttatgcatc gtagtctgga
     3481 agttttaaaa tcaaaaggta tccaaattac tgaacaagaa tctcaagctg ccatactggc
     3541 tattttattg cacgacttgg gtcatgggcc tttttcgcat gcgctggaag gtattttgct
     3601 gaaaaattac catcacgagg atatttctat cttgttaatg gacaagctaa aaatgatagc
     3661 aactgagccg atagaaatgg ctattgccat ctttaaaaat acttatccca aaaaatttct
     3721 gcaccagtta gtaagcggtc agcttgatat ggacagaatg gattacctca atcgggattc
     3781 cttttatacc ggagtcagtg aaggtgtaat cggctatgac cgtatcatca acatgttaca
     3841 tgtacatcat gataatatcg tggtggaaga aaaagggatt
//
LOCUS       DAOQII010000246         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14593_length_3861_cov_4.351025, whole genome shotgun sequence.
ACCESSION   DAOQII010000246 DAOQII010000000
VERSION     DAOQII010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14593_length_3861_cov_4.351025"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1686
                     /gene="ccoG"
                     /locus_tag="PLF48_10260"
     CDS             286..1686
                     /gene="ccoG"
                     /locus_tag="PLF48_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNF49753.1"
                     /translation="MDLNELEHAETYRDRISTVSEKGERKWVYASKPSGKFYTYRKIV
                     VFIYVATLIILPFIKVNGMPFVQINLPEGRFILFSKIFWPQDFYIFAIGMIAFIMFIA
                     LFTVIYGRLFCGWVCPQTIFMEFIYRPIEWMVEGNPASQRLLNNAPWSGSKIAKKITK
                     HVLFLIVTVLISNIFLSYIFGVEKLWDYMKHPADHISLLIGIGVFSLFFYFVFAFVRD
                     IVCTTICPYGRLQGTLFDKDTMQIAYDYKRGEPRGKINKKAERTIGDCIDCKHCVHVC
                     PTGIDIRDGLQMECVGCTACIDACDEMMEKVHLPKGLIRYASENEIASGEKWHFTNKV
                     KAYSVVLAILSIAMFVLLLTRKDLDVNVARAAGQLYNIEADGKLSNLYNLRFLNKLEK
                     QYPVTLKLENMDGKLEVVGGNDIVLKKADITNATLIIHLARKDLNGYKTKVLIGVYHG
                     DKKIRTIKASFIGPMM"
     gene            1696..2133
                     /locus_tag="PLF48_10265"
     CDS             1696..2133
                     /locus_tag="PLF48_10265"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNF49754.1"
                     /translation="MSWGYRILILYVVFVLGIVFMIYKANTYKVELEDKDYYAEELKF
                     QSVLDATKNAKALSTHPVMRDSSANIVVVLPHEFSSVAASGDLRFIRLDDQKKDRAFK
                     INTSSAQQLIPKAGLASGLYKVKLRWKANNTDYYYEDSYTVLP"
     gene            2130..2849
                     /locus_tag="PLF48_10270"
     CDS             2130..2849
                     /locus_tag="PLF48_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF49755.1"
                     /translation="MISSITTGFLIGLMGSFHCAGMCGPLALALPIHTDNKWNRFFLS
                     LSYNLGRVFTYSFLGLIFGLLGKKLFIGKGQQYFSIVLGVLILLAVIKIYLLPKVNIP
                     LPLYNKFNAFIKKNIARYIKNDDAISLLIIGVLNGFLPCGLVYVAIATALAAGSLSGS
                     ILIMAFFGLGTLPMMSFIVFVGKSISFNWRLRIQKLIPVFISAMAVILILRGLNLGVP
                     YLSPKLEKSAEGTEAECCHRK"
     gene            complement(2837..3790)
                     /gene="queG"
                     /locus_tag="PLF48_10275"
     CDS             complement(2837..3790)
                     /gene="queG"
                     /locus_tag="PLF48_10275"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNF49756.1"
                     /translation="MMDFFILNKNEFSRLIKQKASQLGFTACGIAKSGFLDEDAPRLE
                     NWLSNNLHGEMKFMENHFDKRLNPSLLVDNAKSVISLSYNYYSGKKQLADAPKISKYA
                     FGEDYHHVVKDKLKILYAYMQEIYGEFHGRLFVDSAPVMERSWAQKSGIGWIGKHSLL
                     IQKARGSFFFLAEIICDIELEYDEPFHTDHCGNCTACMDACPTEAIITDKVVDASKCI
                     SYLTIELKGDLPIAFQDKLEDWMFGCDICQDVCPWNRFAIQHQEEKFEPQELLLKMNR
                     KEWTDLKEETYNLLFQKSAVKRTKYLGLKRNIDFVNKAYFR"
BASE COUNT         1174 a          684 c          735 g         1268 t
ORIGIN      
        1 aggatgtagc ctcttatgtg aaaacattgc aaggcactaa tcctccggga gctaaagaaa
       61 aacaaggtac agaatgctct gatgctggtg cacctacagc tgctgccggt gctgataaag
      121 cggctgaacc tgctaaagat tcagttactg ctgctaagta gttaacagaa attataacta
      181 aaccacataa ccccttatct ttatgttgta catatcgtaa gagtgtgtta tgtggttttt
      241 ttattgaaat agtattgcta aattaaacgc ttaaataatt gcaaaatgga cttaaatgaa
      301 ttagaacatg cagaaaccta cagagacaga attagtacgg taagtgagaa aggagaacgc
      361 aaatgggtat atgcatcaaa accgtcaggt aaattttata cctaccgaaa aatagttgtc
      421 tttatttatg tggcaacatt gattatactt ccatttatta aagtaaacgg aatgcccttc
      481 gtgcaaatca atttacctga agggcgcttt atcttgtttt caaaaatatt ctggcctcag
      541 gatttctaca tttttgccat tggcatgatt gcatttatca tgtttattgc actctttaca
      601 gttatttatg ggcgtttgtt ttgtggttgg gtatgtccgc aaacgatttt catggaattc
      661 atttaccgcc caatcgaatg gatggtggaa ggaaatcccg cttcgcagcg acttttaaac
      721 aatgcacctt ggtcggggtc taaaattgcg aaaaagataa ccaaacatgt cttatttctt
      781 attgttactg ttttaatttc aaacatattt ttatcatata ttttcggagt agaaaaacta
      841 tgggattata tgaaacatcc tgctgatcat atttctttac tcatcggaat aggagtattc
      901 agtttgttct tttattttgt atttgctttt gtccgggata tcgtgtgtac taccatttgt
      961 ccttatggtc gtttacaagg aaccttattt gataaagata ctatgcagat tgcttatgat
     1021 tacaaacgcg gtgaacccag aggtaaaatc aataaaaaag cagaacgcac tatcggagat
     1081 tgtattgatt gtaaacactg tgttcatgtt tgtcctaccg gaattgatat cagagacggt
     1141 ttacaaatgg aatgtgtggg ttgtacggca tgcattgatg catgtgatga aatgatggaa
     1201 aaagtacatt tgcctaaagg attaattcgc tatgcatctg aaaatgaaat tgcatccggc
     1261 gaaaaatggc attttaccaa taaagtaaaa gcatacagtg ttgtacttgc catactgagt
     1321 attgccatgt ttgtcttatt attaacaaga aaggacttgg acgtaaatgt ggctcgtgcc
     1381 gcaggacagt tatataatat tgaagcagac ggcaaattaa gcaacctgta caatttacga
     1441 tttttaaaca aactggaaaa acaatatccg gttactctga aattggaaaa tatggatggc
     1501 aagctggaag ttgtgggtgg aaatgatata gttttgaaaa aggctgatat aaccaatgct
     1561 actttaatta tacatttagc gagaaaagac ctcaacggat ataagacaaa agtattgatt
     1621 ggtgtatatc acggagataa aaaaataaga acaattaagg cttcctttat cgggccaatg
     1681 atgtaaattt atgctatgag ctggggatat cgcattttaa ttctttacgt tgtttttgta
     1741 ctgggcatcg tatttatgat ttacaaagcc aatacatata aagtagaact tgaagataaa
     1801 gactattacg cagaggaatt gaaatttcaa tctgttttgg atgctaccaa gaatgccaaa
     1861 gcattgagta cacacccggt aatgagagac agctcagcaa atatcgttgt tgttttgcca
     1921 cacgaattct caagcgtagc ggcttcaggt gatttgcgtt ttattcgttt ggatgatcag
     1981 aagaaagacc gtgcttttaa aataaatacg tcatcagctc agcaactcat accaaaagca
     2041 ggcttggctt ccggattata taaggtaaaa ctgcgatgga aagccaataa cacggactac
     2101 tattacgaag acagttatac cgttcttcca tgattagcag tattaccaca ggttttctta
     2161 tcgggttaat gggtagtttc cattgtgcag gcatgtgcgg gccattagca ttggcattac
     2221 ccattcatac tgacaataaa tggaatcgat tttttttgag tttgtcatat aacctgggca
     2281 gagtatttac ctattctttc ttgggattaa tatttggatt actcggtaaa aaattattca
     2341 ttggcaaagg tcagcagtat ttttctattg ttttgggcgt actgatatta ctggcagtca
     2401 tcaagattta cttgcttcca aaagttaata tcccgttacc attgtataat aaattcaatg
     2461 cctttattaa gaaaaacatt gcacgatata ttaaaaacga tgatgctatt tcactattaa
     2521 ttataggcgt actcaacggt tttctgccat gtggtttggt ctatgttgct attgcaacag
     2581 cattagccgc cggttcactt tcgggaagta ttttaatcat ggcatttttc ggactcggaa
     2641 ctttaccgat gatgagtttt attgtttttg taggaaaatc tatttctttc aattggcgtt
     2701 tgcgcattca aaaactcatt cctgttttta tttcagcgat ggctgtgatt ttaattttgc
     2761 gcgggttaaa cttaggcgtt ccctacttat ctcctaaatt agaaaaatct gcagaaggaa
     2821 ctgaagcaga atgttgtcat cgaaaataag ctttattcac aaagtcaata ttccgtttta
     2881 atcctaaata ttttgttctt tttaccgctg atttctgaaa taacagatta taagtttctt
     2941 cttttaaatc cgtccattct tttctgttca ttttgagtag tagctcttgt ggttcaaatt
     3001 tttcttcttg gtgttgtatg gcaaaacgat tccacggaca aacatcctga cagatatcac
     3061 aaccaaacat ccagtcttcc aatttatctt gaaatgcaat tggcaaatct cctttcagtt
     3121 ctatggttaa atatgaaata cacttggacg catctactac tttatctgtt ataatagcct
     3181 ctgtaggaca agcatccata caagcggtgc aattgccaca gtggtcggta tgaaagggtt
     3241 catcgtattc cagttcgata tcacaaataa tttcagctaa aaagaaaaaa gaacctctcg
     3301 ctttttgaat gagtaaactg tgttttccta tccagccaat acccgatttt tgtgcccagc
     3361 tcctttccat taccggcgcc gaatcaacaa acaaacggcc atgaaattcg ccatatattt
     3421 cctgcatata tgcatacaag attttcaatt tgtcttttac cacatgatga taatcttctc
     3481 caaaggcata cttggaaatt ttcggtgcat ctgcaagttg ttttttaccg gagtaatagt
     3541 tatagctcaa ggaaatgact gactttgcat tatcaactaa caaacttggg tttaatcttt
     3601 tatcgaaatg attttccata aacttcattt cgccatgcag attattgctt agccagtttt
     3661 ctaaccgggg tgcatcctca tccaagaatc cggatttggc aataccgcaa gccgtaaagc
     3721 ctaattgcga ggctttctgt tttatcagcc tggaaaactc atttttgttc aaaataaaaa
     3781 agtccatcat tactgatgga cttgctttgg ttagtcggga tggctggatt cgaaccaacg
     3841 acctcctggt cccaaaccag g
//
LOCUS       DAOQII010000247         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14651_length_3851_cov_4.443625, whole genome shotgun sequence.
ACCESSION   DAOQII010000247 DAOQII010000000
VERSION     DAOQII010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14651_length_3851_cov_4.443625"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLF48_10280"
     CDS             <1..618
                     /locus_tag="PLF48_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF49757.1"
                     /translation="KGTPGMEVVRLEKKLGIKASDTAAIVFTECKVPAANLLGSPDID
                     IKKGFGGVMETFDNTRPFVASMANGVAEAALERTKELLVDKVNLDYKTPINNVKAAEA
                     ILYRMEADYEAARLLTLKAAWMADNKMPNTLEASICKAKAARITNELTLKCVELCGTL
                     GYSYDELLEKWSRDSKILDIFEGTQQIQQLIIARRILNKSSSELK"
     gene            632..2458
                     /locus_tag="PLF48_10285"
     CDS             632..2458
                     /locus_tag="PLF48_10285"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HNF49758.1"
                     /translation="MSKLSPAKKNISLFEIISKLPGLTKDIPKFATALFYNYTIQPDT
                     EISFGTFLRDAANRFPHHICLRYHHKTWTYTAFNLWVNRLAHQFTSIGIRKGDVVALL
                     IENRPEILAIPMALAKIGAVTALINTSQRNQTLLHSFQLSKAKLILIGDELLSHFNDI
                     AQDKSLHDIPAYSIKHPAYHPKKNSLPVFDSYAHTYFEHEPQASERVTAKDACLYIYT
                     SGTTGLPKASVISHGRWIKGYAAFGLVNLRLTSTDILYVPLPFYHATAMVVCWSSVIA
                     GGASIVIKNKFSVSDFWQDIKQYRATAFAYVGEMCKYLLNASVSPLEKNNTLIKMIGN
                     GLRPDIWNAFKMRFGIEQIAEFYGSSEGNIAFFNVFNLDNTMGFSNTEYAIIEYDAEN
                     EVPVLDKRGFHKKVKKGGTGLLLGEISKRYPYDGYTENAKTEKTILRNVFKQGDAWFN
                     TNDLVRDLGFGHTQFVDRLGDTFRWKGENVSTQEVEGIINQFEGIAECTVYGVEIPNY
                     NGKAGMANLILKDNVAAEFNLEHFYQQLAKQLPAYAIPVFLRFSSTVDKTDTFKHKKQ
                     QLKQEAYRPNLMQEEVYLLNNKHYEILDAALLKKIEYGKFRI"
     gene            complement(2475..3485)
                     /locus_tag="PLF48_10290"
     CDS             complement(2475..3485)
                     /locus_tag="PLF48_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF49759.1"
                     /translation="MNNKYLTIFLIILFIKINDMNSQSNLKLLDIQLANYDYGYTSKT
                     LTFESQNQTLEMSYIYEKPDNFNGQHVMLLHGKNFNGAYWQTTIQALLKEGFAVVVPD
                     QIGFGKSTKPLHFQYTFQELAKNTKLILEHEHIDKISVLGHSMGGMLATRFTLMYPET
                     VDKFIMENPIGLEDWKLKVPYRPINFWYANELKANYESLKNYQLVSYYDNHWKPEYDE
                     WVNILAGWTLNSQYPLIAWNNALTFEMIFTQPVVYEFPNIKNKTLLIIGTRDRTAMAT
                     FLVSKEVASTMGRYDLLGKSTQKAIAGSKLVEIPGVGHLPHIEAFDKFIQPLIDFLEG
                     KN"
     gene            complement(3650..>3851)
                     /locus_tag="PLF48_10295"
     CDS             complement(3650..>3851)
                     /locus_tag="PLF48_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNF49760.1"
                     /translation="MPYGAFVEFMPGKQGLLHISEVSHKRLENLDGVLKEGDKIEVKL
                     LGIDNKTGKYKLSRKVLLPKEG"
BASE COUNT         1243 a          685 c          712 g         1211 t
ORIGIN      
        1 aaaggaactc cgggtatgga agtggtgcgt ttagaaaaga aactgggcat caaagcatct
       61 gacaccgccg caattgtttt tacggaatgc aaagtaccgg ctgcaaatct gttgggaagt
      121 ccggatattg atatcaaaaa agggtttggc ggtgtgatgg aaacattcga taacacacgt
      181 ccttttgtgg cgtccatggc taacggtgta gcagaagccg cattagaacg cacaaaagag
      241 ttgctggtgg ataaagttaa tttagattac aaaacaccca taaataatgt aaaagcggca
      301 gaagctatac tatatcgcat ggaagccgat tatgaagctg cgaggttact tactttaaaa
      361 gcagcttgga tggcagataa taaaatgccc aatacacttg aagcatctat atgcaaagca
      421 aaggctgccc gcatcacgaa tgaacttacc ttaaaatgtg tggagttgtg cggtacactc
      481 ggttattcgt atgatgagtt attggagaaa tggtcgagag attcaaaaat tctggatatt
      541 tttgaaggca ctcagcaaat ccagcaatta attatagcaa gaagaatttt gaataaatca
      601 tcttctgaat taaagtaaaa aagtacaggc aatgagcaaa ctttctccgg caaaaaaaaa
      661 tatcagtctt tttgaaatta tctcgaaact acccggttta acaaaagata ttccaaagtt
      721 tgccacagct cttttttaca attatactat tcagcccgat actgaaattt cattcggtac
      781 tttcttgcga gatgcagcca accggtttcc gcatcatatt tgtttgcgtt atcaccataa
      841 aacatggact tatactgctt ttaatttatg ggtaaatcgc ttagcgcatc agtttacttc
      901 aataggcata cgcaaaggtg atgtggttgc tttgttaatt gaaaatcgac ctgaaatatt
      961 agctattccg atggcacttg caaaaattgg tgctgttaca gcattaatta atacatctca
     1021 acgcaatcag acattgttac acagttttca gttatccaaa gctaaactga ttttaattgg
     1081 cgatgaatta ttgtctcatt tcaatgatat tgcacaagat aaaagtttgc atgatatacc
     1141 tgcatacagc attaagcatc cggcatatca tccgaaaaaa aacagcttac cagtttttga
     1201 cagttatgca catacttatt ttgaacatga accacaggct tctgaacgtg taactgctaa
     1261 agatgcatgt ttgtatattt atacttccgg cactaccgga ttgcccaaag cctccgtgat
     1321 tagtcatggc agatggataa aaggctatgc cgcttttggt ttggtcaatt tgcgtttgac
     1381 atccaccgat attctctatg tgcctttgcc tttctatcat gctacagcta tggtagtatg
     1441 ctggtcatcg gttattgcag gaggtgcttc cattgtaata aaaaataaat tcagtgtttc
     1501 agatttttgg caagatataa aacaatatcg cgccactgct tttgcctatg tcggtgaaat
     1561 gtgtaaatac ttactaaacg catctgtttc gcccctcgaa aaaaataata ccttaataaa
     1621 aatgattggc aacggcttgc gaccggatat ctggaatgca tttaaaatga gatttggtat
     1681 agaacaaata gcagagtttt acggatcgag tgaaggcaat attgcgtttt tcaatgtctt
     1741 taatttagac aacacaatgg gattttccaa taccgaatat gctatcatag aatatgatgc
     1801 ggaaaacgaa gtgccggttt tggacaaaag aggttttcat aaaaaagtaa aaaagggcgg
     1861 tactggtttg ttgttaggcg aaatctctaa acgctatcct tacgatggtt atacagaaaa
     1921 tgcaaaaact gaaaagacta ttttgcgtaa tgtttttaaa caaggcgatg catggtttaa
     1981 taccaatgat ttagttcgcg atttgggttt cggacatacg cagtttgtag atcgcttagg
     2041 agatactttc cgatggaaag gggagaacgt ttctacgcaa gaagtagaag gtattattaa
     2101 tcaatttgag ggcattgccg aatgtacggt gtatggtgta gaaataccta actacaatgg
     2161 aaaagcagga atggcaaatc tgatactaaa ggacaatgtt gctgctgagt ttaatttaga
     2221 acatttttat cagcaattgg caaagcaact gccggcttat gcaataccgg tattcctgcg
     2281 ttttagttca acagttgata aaacagatac attcaagcat aaaaaacagc aacttaagca
     2341 agaagcatac cgaccaaatt taatgcagga agaagtttat ttactaaata ataaacacta
     2401 tgaaatctta gatgctgctc ttttaaagaa aatagaatat gggaaattca gaatttagaa
     2461 attagcctga taaattaatt cttgccttct aaaaaatcaa taagtggttg tataaattta
     2521 tcgaatgctt caatatgcgg gagatgccct acacccggaa tttctacaag tttactgcca
     2581 gctatagctt tttgtgtgga tttacccagc aaatcatatc gccccattgt cgatgctact
     2641 tctttagaaa ctaaaaatgt tgccatggca gtccggtcac gtgtgccaat aatcagcagc
     2701 gtcttatttt tgatattagg aaattcgtaa actacaggtt gtgtgaaaat catctcaaat
     2761 gtaagcgcat tgttccaggc aattaacgga tattgtgaat taagtgtcca accggctaaa
     2821 atatttaccc attcatcata ttccggtttc caatgattat cataataact tactaattga
     2881 taatttttca gtgactcata atttgctttt aattcattag cataccaaaa attgataggc
     2941 cgataaggca ctttcagttt ccagtcctcc aagccaattg gattttccat aataaattta
     3001 tctactgttt caggatacat taacgtaaac ctagtcgcaa gcatacctcc catagaatgt
     3061 cccagtacgg aaatcttatc tatatgttca tgttctaaaa tgagcttggt gttttttgct
     3121 aattcttgaa aagtatattg aaaatgtaaa ggcttggttg attttccaaa accaatctga
     3181 tctggaacga ctacagcgaa tccttctttt aaaagtgctt gaatagttgt ttgccagtat
     3241 gcaccgttga aatttttccc atgcaaaagc atgacatgct gaccattgaa attatcaggc
     3301 ttttcataaa tatatgacat ttccaatgtc tgattttggc tttcaaaagt cagtgtttta
     3361 ctggtatagc cataatcata atttgcaagt tgaatatcta gtaattttag atttgactgt
     3421 gagttcatat cgtttatttt tataaataag ataattaaaa atatggttaa atatttgtta
     3481 ttcataatct gaatttatta gttcagaaat aaaaatacaa cttttttgct tacattttta
     3541 tacagtacaa atacaccatg cataatgact tctttagttc aaatatctac aaaaacaaac
     3601 atggcgcgcc gaaggcgcgc catgactgtc tctattcttt caaaaaatat tatccttctt
     3661 taggtaataa taccttgcgg cttagtttgt acttccctgt tttattgtcg atgcccaaca
     3721 attttacttc tatcttgtcg ccttctttca aaacgccatc caaattttct aagcgcttgt
     3781 gagatacttc tgatatatgc aacaagcctt gtttgcccgg cataaactca acaaatgcgc
     3841 catacggcat t
//
LOCUS       DAOQII010000248         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14724_length_3838_cov_4.849062, whole genome shotgun sequence.
ACCESSION   DAOQII010000248 DAOQII010000000
VERSION     DAOQII010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14724_length_3838_cov_4.849062"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PLF48_10300"
     CDS             <1..914
                     /locus_tag="PLF48_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49761.1"
                     /translation="PNKWVKVLDGVKSVTAGYQETFIIKNDNTVWATGRNLSGNLGDG
                     TKDMRTSFVKVADNGKAVFACRYNTFILKNDNSLWATGKNEDGMLGIGATPAETSTFV
                     KVTDNVKSVAVGTWHTLILKTNGTVWAVGFNNGGRLGDGTYVTKTELVKVADNITAVA
                     AGYSYSLLLKNDKTVWACGWNDVGQFGITTSDLGVPTFIKVAENVNYIAAGVHSEYSV
                     IIKTDNTLWVTGSNGYGQLATGNTNTLLTWVKAADSVKAMSAGTAHLTIQKTDGTLWA
                     AGANDASQLGTDEATAPKTSFVKVVIP"
     gene            976..1581
                     /locus_tag="PLF48_10305"
     CDS             976..1581
                     /locus_tag="PLF48_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49762.1"
                     /translation="MKAKLHKLKKLLSNLLSKKTIIIFYITSFLFLSCNNSKSKSTTV
                     EDVIQKAKETTAVVAKEGRGNISLQCSGKTYEINGVCGALTSMGSVSIVVKDDSTKLK
                     VFTIDFSTDQLPTSSSSYKIIKNADDKDAHYVSIGYNDMRQPQMIWESDNATGKLDFT
                     VNGDEIKCSFSNLKLQANEFFNKGELNNPAIVSGELTLYKN"
     gene            1719..2129
                     /locus_tag="PLF48_10310"
     CDS             1719..2129
                     /locus_tag="PLF48_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49763.1"
                     /translation="MNRIILLFAMTILLAIGCKKDKVQTELPANYTNQGTITVAKKNI
                     SICIYDNSVEDGDIVDLIFNGKALVTNYELKNAEKCFNVTLENGNNWIGIIAKNEGTN
                     PPASVTVKINDGVSEQSFDIDGEIDHPGGYIIKL"
     gene            complement(2306..3760)
                     /locus_tag="PLF48_10315"
     CDS             complement(2306..3760)
                     /locus_tag="PLF48_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49764.1"
                     /translation="MKEIRLVQLIHALSSSDIDFVKTQIKAQQNKMYLPLFTAIHLLT
                     QTPFNREEIYLRVYKKKWTPKGDAIFRTDQSRLADFIEDILMQKRWKAQIKNDEEWQQ
                     EERINLYLDLKLFKEAEYVYNEISTAVSYSFSGKNRINDKYADYLMKSHLPLKEKERL
                     MGVITKNYLQNSQQEAEIRQAKSWYLKCMHNYYHKQLTNTFFEEIVVEEVLKIADEFV
                     NEEAKLIILNGIAYLKIDSPATDISLHIYEETVRLATILVNYHPRFLHYKAKALHLIG
                     TRYSILGDFKNGNKYFEQAIIIIPKEQYLNYKTIFLNYATNCSKQKQFAKAIELIHLL
                     EEEAARDSKLKTECAIRSLSCYLFMENAPAIHALVNSQDYNLIQPHEKIYFRLCQCNA
                     FLMENEFEFAHSEITNLLRSKLMQEIDVNFIPATELMNFMIAAVFKNGKIKLTPAQVQ
                     RYEKVKAALNIQQFPYLRHYSPFLWLEQKLLPQH"
BASE COUNT         1264 a          646 c          711 g         1217 t
ORIGIN      
        1 atcctaataa gtgggtaaaa gttttagatg gtgtaaaatc tgttaccgca ggttaccaag
       61 aaacgtttat cattaaaaat gataataccg tttgggcaac aggacgtaat ttgtccggta
      121 atttgggcga tggcactaaa gatatgagaa cttcttttgt aaaggttgcg gataacggca
      181 aagccgtttt tgcatgtcgt tacaacactt ttattcttaa aaatgataat tcactttggg
      241 caacaggcaa aaatgaagat ggaatgttag gcattggtgc aacacctgcc gaaacaagca
      301 cttttgtaaa agtaacggac aatgtaaaat ctgtagccgt gggaacttgg catacactta
      361 ttttgaaaac aaatggtact gtatgggctg ttgggtttaa taatggtgga cgattaggcg
      421 atggcactta tgtaactaaa acagaattag taaaagttgc tgataatata acagcagttg
      481 cggcaggata tagttatagt ctgttactta aaaacgataa aacagtttgg gcttgcgggt
      541 ggaacgatgt agggcaattc ggcattacca cctcagacct tggggtacca acatttatca
      601 aagtagcaga aaatgttaac tatattgcag caggcgtaca tagtgaatac agtgttatca
      661 taaaaacaga caatacactc tgggttacgg ggtcgaacgg ttacggacaa ttagccacag
      721 gaaataccaa tactctactt acttgggtaa aagcagcaga cagtgtaaaa gcaatgagtg
      781 ccggtaccgc tcatcttact atccaaaaaa cagacggcac actatgggca gccggtgcaa
      841 atgatgcttc tcagttaggc acagatgaag ctactgcccc aaaaacctct tttgtaaaag
      901 ttgttattcc ttaattattc atcctgaaat ataaaacaga gaaaataaat taatgcacaa
      961 aaaaaatcac agaaaatgaa agcaaaactt cacaaactca aaaaattact ttctaattta
     1021 ttgagtaaaa aaacaatcat tattttttac ataacaagtt ttcttttctt gtcgtgcaat
     1081 aatagcaaaa gtaaaagcac tacggtagaa gatgttattc agaaagccaa agaaacaacg
     1141 gcagtcgtag ccaaagaagg acgtgggaat attagtttgc aatgcagtgg aaaaacttac
     1201 gaaatcaacg gcgtatgtgg tgcattaacc agtatgggca gtgtttccat tgtcgttaaa
     1261 gacgatagca ccaagctaaa agtatttact attgatttct caaccgacca gttgccaacc
     1321 tcttcctctt cttacaaaat tataaaaaat gctgacgata aagatgccca ttacgtatcc
     1381 ataggctaca acgacatgcg acaaccacaa atgatttggg aatcggacaa tgccaccgga
     1441 aaattagatt ttacagtaaa tggcgatgaa ataaaatgct cgttttccaa cttgaaatta
     1501 caagcaaacg aatttttcaa taaaggtgaa ctaaataatc cggctatagt ttccggcgaa
     1561 ctaacattgt ataaaaatta aaaaagagaa tctaaaatgc gagccgtctt ttcgagcgag
     1621 gtacgagtga gtttcgaacg ttcggaatga cggttctcat taaagtgtga gattcaataa
     1681 cgacaaaaaa attaataact tgctaaaaaa caaaaagaat gaacagaatt attttattat
     1741 ttgcaatgac aatcttgtta gcgattggct gcaaaaaaga caaagtacaa acagagttac
     1801 ctgccaacta cactaatcaa ggtactatca cagttgccaa gaaaaacatt tctatctgca
     1861 tttatgataa cagcgtagaa gatggcgata ttgttgattt aatatttaac gggaaagcat
     1921 tggtaacaaa ttatgaattg aaaaacgcag aaaaatgctt caatgttact ttagaaaacg
     1981 gcaataattg gattggcatt attgctaaaa atgaaggcac aaatcctccg gcatctgtta
     2041 ccgtcaaaat aaatgatggt gtaagtgaac agtcattcga cattgatgga gaaatagacc
     2101 atccgggtgg ttacatcatt aaactataag aagaattttt attcaataat atctgtcttt
     2161 ggtagaatgt aaaatagttt gatagctgat attgagtatt gcctgttttt actcattttt
     2221 cattttttgt aagcgaagcc ttgcggcttc gcttttttat aaaaaaaatc cgaaacagtt
     2281 tgtttcggat tctattctat cattctcagt gctgtggtag gagtttttgt tctaaccaaa
     2341 gaaacgggga ataatgtctt aaatatggaa attgttggat attgagtgct gctttcactt
     2401 tttcatatcg ttgtacttgc gccggcgtta gctttatttt tccattttta aaaaccgcag
     2461 ctatcataaa gttcatcaat tctgttgccg gaataaaatt gacatctatt tcctgcatca
     2521 acttggaacg caacagattg gttatttcgg aatgggcaaa ctcaaattca ttttccatca
     2581 aaaaagcgtt gcattgacac agtcggaaat aaattttttc gtgcggttgt attaaattat
     2641 aatcttggct attcactaag gcatgaatgg ctggcgcatt ttccataaaa agataacaac
     2701 tcaaactgcg tatggcgcat tctgttttta attttgaatc tcttgcggct tcttcttcca
     2761 ataaatgtat taactcaatc gctttagcaa attgtttttg cttgctgcaa ttcgttgcat
     2821 aattaaggaa gatagtttta taattcagat actgttcttt ggggattatt attatggctt
     2881 gttcaaaata tttatttcca tttttaaaat ctccgagtat agaatagcgc gtgccgataa
     2941 gatgaagtgc ctttgcttta taatgcaaga agcgcggatg ataattaacc aaaatggttg
     3001 ccagccggac ggtttcttcg taaatgtgca atgaaatatc cgttgccggg ctgtcgattt
     3061 tcaggtaggc aatgccgtta agtataatca atttagcttc ttcatttaca aattcatctg
     3121 caattttcaa tacttcttct acaacaattt cttcgaaaaa agtatttgta agttgcttgt
     3181 gataataatt atgcatgcac tttaaatacc acgattttgc ctgccgtatt tctgcttctt
     3241 gctgagaatt ttgcaaataa ttttttgtaa tcactcccat tagccgctct ttttctttga
     3301 gtggcaaatg gcttttcatc aaatagtctg catatttatc gtttatccta tttttgccgg
     3361 aaaatgaata tgaaacagca gtcgaaattt cgttgtacac gtactcggct tctttaaata
     3421 attttaaatc gaggtacaaa tttattcttt cttcttgctg ccattcttca tcatttttta
     3481 tttgcgcttt ccaacgtttt tgcatcagta tatcttcaat aaaatctgct aagcgacttt
     3541 gatctgtgcg aaaaatggca tcgcctttgg gtgtccattt ttttttatac actcttaaat
     3601 atatttcttc acggttaaat ggtgtttgtg ttagcagatg gattgcggta aataatggca
     3661 gatacatttt attttgctgc gcctttattt gggttttaac aaaatcaata tctgagcttg
     3721 acaatgcgtg aataagttga acaagacgta tttctttcat accatttgga aaaatacaat
     3781 ttaatagtaa atattccaaa gtccgttttg gtgttattca ccaaaacata aactaaag
//
LOCUS       DAOQII010000249         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_14975_length_3793_cov_4.479401, whole genome shotgun sequence.
ACCESSION   DAOQII010000249 DAOQII010000000
VERSION     DAOQII010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14975_length_3793_cov_4.479401"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..736
                     /locus_tag="PLF48_10320"
     CDS             113..736
                     /locus_tag="PLF48_10320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNF49765.1"
                     /translation="MMELSKTYKIVTERLIIRCYEPCDASKFKQAIDSSIAHLLPWMP
                     WAKDEPEDISVKVKRLRLYRGQFDLGIDYTFGIFDKNEKVLIGSTGLHTRLGINAREI
                     GYWIAYDYINKGFATESTMALTKVGFELEYLEKIEIHCSVDNLKSQNIPRKIGYKLEK
                     KLKMDILVQDDAVVSKMIWTMHKEDYENSELKNFELKAFDNCGNRLI"
     gene            1000..2145
                     /locus_tag="PLF48_10325"
     CDS             1000..2145
                     /locus_tag="PLF48_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49766.1"
                     /translation="MKTTLKLMTAAIALLLLSNQHAQAQFGKKLKEALVGKPAQNETK
                     SDANASSATTEKAVDNSYVLTDFQKANVGKVVIFNDYLGQRLDESAADAASITELADH
                     FILRAYFDKNVRDTYDKGFDIVFSCEDVKVSLNQLRYYSTENFMKAGGPTGKTPGILA
                     LYYDMADLKYKNLWKNEKIFSTGLMPKKMDSEYDAAQYKYYPVENAFRFFLNKLSSKI
                     AVGKTLTIKIQFMGINSSAADGWKATDAEKTVLAEGTHNIKITANTTKLTNNVYRFEE
                     EDYINKPTVNENIKKALKLKYGAEVAEVYKVQMVSDFTTKSNVYGPEYQWIQGRVLLK
                     AKADGSYWACKFEAHFKYNGSGFDNAPFEIIFTRWALVPSVGIPGAK"
     gene            2629..2985
                     /locus_tag="PLF48_10330"
     CDS             2629..2985
                     /locus_tag="PLF48_10330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF49767.1"
                     /translation="MKKTTKLLSIVALVATLFFAACKKEDTPAPVADFTILPNDTIEF
                     FDTLRLTNTSTNADTYLWNFGDGTTSTLTNPIKASFNRPGTSTGCTETYTITLTATKN
                     GKSSTKSKTVLVGYCL"
     gene            complement(3168..>3793)
                     /locus_tag="PLF48_10335"
     CDS             complement(3168..>3793)
                     /locus_tag="PLF48_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF49768.1"
                     /translation="EGNIGLIKAAKKFDETKGFKFISYAVWWIRQSILQALIEQPRVV
                     RVPMNKATIYNKIQRAIVDFEQQNQREPSNDELAELLDMKPEELSMLRATLSYHLSVD
                     TPIGDDDDTTSLDLMTDNTLGNPEKNLIDESLHDELEHALQSLTDRERDILKLYFGIN
                     EFNQSYSLDEIGLKLNLTRERVRQLKDKAIRKMRRFAISKELKAYLS"
BASE COUNT         1362 a          599 c          647 g         1185 t
ORIGIN      
        1 aagacgcagt tttgtgatat gtgtgtttat ggagaacttt tttgggttat tttacttata
       61 tttctccaat atgacttaaa ttttctaatt tagtaattat ataattaaac agatgatgga
      121 attatctaaa acttacaaaa ttgtgacaga aagattaata ataagatgct acgagccctg
      181 cgatgcatct aaatttaaac aggctattga ttcaagtatt gcgcatttac ttccgtggat
      241 gccttgggca aaagatgaac cagaagacat tagtgtcaag gtaaaaagat taagactgta
      301 tcgtggtcaa tttgatttgg gaattgacta cacttttggc atttttgata aaaatgaaaa
      361 agtactaatt ggttcaacag ggcttcatac aagactaggg ataaatgcca gagagattgg
      421 gtattggatt gcttatgatt acattaataa agggtttgct actgaaagca caatggcatt
      481 aacaaaagta ggatttgaac tggaatatct cgaaaagatt gaaatacatt gtagtgttga
      541 taatctgaaa agccaaaata ttccaaggaa aattggttat aaattagaaa aaaaattaaa
      601 aatggacatt ttggttcaag atgatgctgt tgtttctaag atgatatgga ctatgcataa
      661 agaagattat gagaacagtg agctcaaaaa ctttgaatta aaagctttcg ataattgtgg
      721 taacaggctg atttgaaaaa taatcaaata gtaaaaatgg tatctctgat attttccatt
      781 aaaattacaa caagcatatt ggtgtcagtt cctaattaga aaaaaggacg ttcagtatgc
      841 acatttcttg ttcaaaatac caatcgtatt ggttgcaata tactatgtgt cttatcccat
      901 aattgttatt tatgaaatga aatcaacagt ttatatagtt gcttgaaaat atgggcgcac
      961 gctcaggcaa ttttgcagtg taataaaata aagacaatta tgaaaactac acttaaacta
     1021 atgaccgcag caatcgcatt gctattatta agcaatcagc atgcacaagc tcaatttggc
     1081 aaaaaactaa aggaagcact ggtaggcaaa ccagctcaaa atgaaactaa aagtgacgca
     1141 aatgcatcat cagctactac tgaaaaagca gttgataatt cgtatgtact tacagatttc
     1201 caaaaagcaa atgttggaaa agtcgtcata tttaatgatt atttaggaca aagacttgat
     1261 gaaagtgcag ctgatgcagc aagcataacc gagcttgctg atcattttat tttgcgtgca
     1321 tatttcgata aaaatgttcg tgatacttat gataaggggt ttgatattgt attcagttgc
     1381 gaagatgtaa aagtaagttt aaaccaactt cgatattatt caactgaaaa tttcatgaaa
     1441 gcaggtggac caactggtaa aactcccgga attttggctc tttattatga tatggcagat
     1501 ttgaaatata aaaatttatg gaaaaacgaa aaaatatttt ctacaggatt gatgcctaaa
     1561 aaaatggatt cggagtatga tgcagcccaa tataaatact atcctgttga aaacgcattc
     1621 cgttttttcc tcaataaact aagcagtaaa atagcggttg gaaaaacgct aaccattaaa
     1681 attcaattta tgggaatcaa ttcatctgct gcagacggat ggaaagcaac agatgcagaa
     1741 aaaacggttt tggcagaagg aacacataat ataaaaatta cggcgaatac gactaagctg
     1801 accaataatg tatatcgttt tgaagaggaa gattatatca ataaaccaac tgtgaatgaa
     1861 aacataaaaa aagcactgaa actaaaatac ggtgctgaag tagcagaagt ttataaagta
     1921 caaatggttt cagattttac aaccaaatca aatgtatatg gcccggaata ccaatggata
     1981 caaggaagag tacttctaaa agccaaagct gacggtagtt actgggcttg taaatttgaa
     2041 gctcatttta agtataatgg ctccggtttt gacaacgctc catttgaaat catttttacc
     2101 agatgggcat tagtaccttc tgtaggaata ccgggtgcaa agtaaatgaa ctgaatttat
     2161 ccattcgaat aactaaaacc ataaaactat taaaaaaata attgttcttt tcattaacaa
     2221 ttgttttgga gcgatactga aaggtattgc ttttttattt attataaaaa tattaaaata
     2281 atactatggt atatatataa ttaactgtat ttcaattaat gtagtttctt tgaatgacag
     2341 cacaagccac attttataaa attacttttc tctactggct atttaagctt aaacaaaaca
     2401 tttttcgttt gcaagatgta acttttattt ttttaccgga tagtccttaa aaaacttcat
     2461 gcaaattata gatagatata gaaatgacag aatgaacttc aaaataataa atctgcttcc
     2521 gtttgtgaat tgaaacctgc cattcgtgaa gtgcaataaa atacagactc atacaatctc
     2581 tatttttgta ctatcaaatt aattaaaaac aatttaaatc aaaacaaaat gaaaaaaaca
     2641 accaaacttc tcagcatcgt agctttagta gctacacttt tctttgccgc ttgcaaaaaa
     2701 gaagacacac ctgcgccggt ggcagatttt actatcttac ccaatgatac catcgaattc
     2761 tttgacactt taaggttgac aaacacttct acaaatgcag acacttactt gtggaatttt
     2821 ggtgacggta caaccagcac actgactaat ccaataaagg cttcttttaa tcgtcccggc
     2881 acaagtacag gatgtactga aacctatacg attacgttga ctgctacaaa aaacggaaaa
     2941 tcttcaacta aatctaaaac agtattagta ggttactgct tgtaaaattt aattcaaaaa
     3001 ctttttagag cgatgacgaa agttgtcgct ctttttattt tataacggca taaatttaaa
     3061 taaattgaac aaagcaaatc tcttagctta agaataatac aagcaaataa aagataaaat
     3121 gagatgaata ttttggaaaa atgaaattta tttttgcgaa atcagtgcta actcaaataa
     3181 gctttcaatt ctttactgat tgcaaaacga cgcattttac gtatcgcttt atctttgagc
     3241 tggcgaaccc gctctctagt caaatttaat tttaagccga tttcatccag cgaataagac
     3301 tggttaaact cattgatgcc aaagtaaagt ttcagaatat cccgttctct gtcagttaaa
     3361 ctttgtaagg catgttcaag ctcatcatgc aatgattcat ctatcaaatt cttttccgga
     3421 ttgcctagtg tattatccgt cattaaatca agactggtgg tatcatcatc atctccgatt
     3481 ggagtatcta cagaaagatg ataggaaagt gtagcacgca gcatagaaag ttcttccggc
     3541 ttcatgtcca gcagttctgc cagttcatca tttgatggtt ctcgctgatt ctgttgttca
     3601 aaatcaacta tagcccgttg tattttgtta taaattgttg ctttattcat gggcacacgc
     3661 accactctgg gctgttcaat cagtgcttgt aagattgact gacgaatcca ccatacggca
     3721 taagaaataa atttaaatcc tttggtttca tcaaattttt ttgctgcctt tatcaatccg
     3781 atattgcctt cat
//
LOCUS       DAOQII010000250         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_15031_length_3784_cov_4.732368, whole genome shotgun sequence.
ACCESSION   DAOQII010000250 DAOQII010000000
VERSION     DAOQII010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15031_length_3784_cov_4.732368"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PLF48_10340"
     CDS             <1..565
                     /locus_tag="PLF48_10340"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNF49769.1"
                     /translation="MYCIAVSILIGLFSHKKEIIALMYPNTDSTYSAKVFGLLLFNFF
                     PIALLYVIGTLLTANKNFKQMIIVLSVAVLINVGLNFILIVKSGAEGAAIATLITQVF
                     MLIAYSVLIMRIFNISFNINYVLRLLSFSVLCLLLVLLTKQLQLQNSKLQLLFQLCGF
                     FICIPILGWISGLLNKEMLHLTFSRMD"
     gene            complement(590..1177)
                     /locus_tag="PLF48_10345"
     CDS             complement(590..1177)
                     /locus_tag="PLF48_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNF49770.1"
                     /translation="MLKIISRILLIAKGWSYNVDLVKPIDKCVLIVAPHTSAWDFALG
                     RAVFTLAGIPAKFAIKKEAFFWPLNWLLRYLGAVPVDRTPKSGNEKKRISLVEAMVNT
                     IKKEKKICMVITPEGSRGKRQQWKTGFYYIALQAHVPIALSYLDFDTKEGIVGKILYP
                     TGNLDNDMREIMDFYRDKTHLGKYPNRCTLDERWA"
     gene            complement(1339..1572)
                     /locus_tag="PLF48_10350"
     CDS             complement(1339..1572)
                     /locus_tag="PLF48_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49771.1"
                     /translation="MESIFTPELLVKFLYNETTPAETLEIKLLLEKDNALKAELEQMR
                     FVKHAMEDFGSDNPSPESISAITAYAKQKEMVG"
     gene            complement(1643..2176)
                     /locus_tag="PLF48_10355"
     CDS             complement(1643..2176)
                     /locus_tag="PLF48_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49772.1"
                     /translation="MTDFALGTKVEHPKYGKGVIIDDSEEYTYTIYFGTEHGEKEISK
                     SFDGLVVIRIAEKDKNSVSLDEIKSVLQDLIEPLTEPLEDCKIAEKWLGGNLIMQPKD
                     RSLQSKEIPIEGFFHKIVMTRDRLRVMEQKINASKLSDAEKVELQQYITRIYGSLTTF
                     NVLFRFKDDQFKGTGGE"
     gene            2425..>3784
                     /locus_tag="PLF48_10360"
     CDS             2425..>3784
                     /locus_tag="PLF48_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF49773.1"
                     /translation="MKTKLLFLAICSACMFTAFAQWGTIGDRILDNMSRKAQNKVENE
                     VDQAAEKSYNKAKEGVKEDVKNSNKNKNSKTKTSDDSSDDDTSTGNDNSSSSKSNSKS
                     NSANSSLKAYGKYDFIPGEKVIAEENFSQDAIGDFPAKWNTNGSGELVTIDGQQGKWL
                     KFGPESVIYPEFVTNLPENFTVEFYVACTQPYSFYSSAFNFFFAPLANPAKNYINWKQ
                     YGSNKNNGVLIAIHPEAAGGAQGQKFLNTYDESGQEIIRNQSDFPDFYHKNGKNVVKV
                     SIWRQKQRLRVYVNETKIWDIPKAFNATAKYNFMGFRTDGYHNEENAYFLSNIRVAVG
                     APDTRHKFLELGKYSTTGIKFDVNSATIKGESYGTLKDFASVMTENPDVKVKIVGHTD
                     SDGDDAKNLELSIKRAEAVKSALNKEFGIDNSRMETEGKGETQPADNNTTPEGKANNR
                     RVE"
BASE COUNT         1224 a          670 c          679 g         1211 t
ORIGIN      
        1 gatgtattgc attgctgttt ccatcttaat aggtcttttt tctcataaaa aagaaattat
       61 tgcactgatg tatcccaata ccgacagtac ttattctgcg aaagtttttg gtttattatt
      121 atttaatttt ttcccgatag ccttgttata tgtgattggc actttattga cggcaaacaa
      181 aaatttcaaa caaatgatta ttgttttgag tgttgccgta ttaataaatg ttggattgaa
      241 ttttatctta atagtaaaat caggcgctga aggtgctgcc attgccacgt taatcactca
      301 ggtatttatg ctgattgcct attctgtatt gataatgcgc attttcaaca tttcatttaa
      361 tatcaattac gttttgcgct tactgagctt cagtgttctc tgcttgctgt tggtattgct
      421 taccaaacag ttgcagttac aaaattcaaa attgcaacta ttatttcagt tgtgcggatt
      481 ttttatttgt ataccgatat tagggtggat aagcggacta ttgaataaag aaatgctgca
      541 tcttactttt tctcgcatgg attaaaattt tcaaaggata ccaattgcct tacgcccatc
      601 tttcatccag ggtgcatctg ttggggtatt tgcccaaatg tgttttatcc cggtaaaaat
      661 ccatgatttc acgcatgtca ttatctaaat tgccagtagg atataggatt ttgcccacga
      721 tgccttcttt agtatcaaag tccagataag ataaagctat gggaacatgc gcctgcaaag
      781 cgatataata aaatccggtt ttccattgtt gtctttttcc tcgagagcct tcaggcgtaa
      841 tcaccatgca tatttttttc tcttttttaa tggtgtttac catagcctca accaagctga
      901 ttcgtttttt ttcattgcca gattttggtg tgcggtctac aggaacagct cccaaataac
      961 gcaagagcca gttgagtggc cagaaaaagg cttctttttt tatggcaaat tttgcaggta
     1021 tacctgcaag ggtaaatacg gctctgccta gggcaaaatc ccatgcggaa gtatgcggtg
     1081 caacgatgag gacacatttg tcaatgggtt ttaccaagtc tacattatat gaccatcctt
     1141 tagctataag taaaatacgg gatattattt tgagcataat ttaaaatatg cggcaaaaat
     1201 aatggaaatt tacgatatgg aattcaccat aaaatattag taaaatatgc ttaagtatgt
     1261 taaatcctaa aaaaataaac tatagttctc aatttggtat aaagtcatta tacctaatag
     1321 tgaaaaaatg agtaaatact accctaccat ttctttttgc tttgcataag ccgtaatggc
     1381 agatatgctt tccggcgacg ggttgtcgct accgaaatct tccatggcgt gttttacaaa
     1441 acgcatttgc tccagttccg ctttcaaagc attgtctttt tctaacagca gtttgatttc
     1501 cagcgtttca gcaggtgtag tttcgttgta caaaaatttg actaacaatt cgggtgtaaa
     1561 gatggattcc ataaaaaaat cgttttatac agttaacgaa aaatagtcat aaatattgta
     1621 catgcaaaat aattctaata ttttattcgc cacccgtccc tttaaactgg tcgtctttga
     1681 aacgaaaaag cacattaaaa gttgtcaaac tgccgtaaat tcttgttata tattgctgta
     1741 attcaacttt ttctgcatca ctaagtttag aagcgtttat tttttgctcc attacacgca
     1801 gtcggtcacg ggtcatcaca attttatgaa aaaaaccttc aatcggaatt tctttgctct
     1861 gcaaacttct gtcttttggc tgcatgatta aatttccacc caaccatttt tctgcaattt
     1921 tgcaatcttc caaaggttcg gtcagcggtt caatcaggtc ttgcaacacg gattttatct
     1981 catcaaggga tactgaattt ttgtctttct cggcaattct tatcacgacc aagccatcaa
     2041 aagatttgga tatttctttt tctccgtgtt ctgtaccaaa gtaaatcgta tatgtgtatt
     2101 cttcactgtc atctataatt acacctttgc catattttgg gtgttccacc ttagtgccta
     2161 atgcaaaatc tgtcatatgc ttaatttttg gctaaaataa gaaaggaaat gctcttttcc
     2221 aatgaccgaa tactaattag taatggacga atactcatta cataaaacca attcacgctg
     2281 acacaacaat ataacccatt gattttttga tatttatgaa ataatataaa aatctctcat
     2341 caggctaaca ttattttggt acttgtgcta tacagaaaat tagtgcaatt ttgaattatc
     2401 attttaaaca caaataaact aacgatgaag actaaattac tttttcttgc catttgttca
     2461 gcttgtatgt tcactgcatt tgcccaatgg ggaactatcg gcgatcgtat cctggataac
     2521 atgagccgca aagcacaaaa taaagtagaa aatgaagttg accaagctgc tgagaaatca
     2581 tacaataaag caaaagaagg ggtgaaagag gatgtgaaga acagcaataa aaataaaaat
     2641 tcaaaaacca agacttctga tgacagttca gatgatgata cctcaacggg caatgacaat
     2701 tcatcttctt cgaaatcaaa cagtaaatca aattctgcta attcttcttt aaaagcttat
     2761 ggaaaatacg atttcattcc cggtgaaaaa gtaattgctg aagagaattt ctcacaagat
     2821 gccattggcg atttccctgc gaaatggaat actaatggta gtggcgaatt ggtaactatt
     2881 gatggacaac aaggcaaatg gcttaagttt ggcccggaaa gtgtaatcta tcccgaattt
     2941 gttaccaatt taccggaaaa cttcactgta gaattttatg tggcttgtac gcaaccttac
     3001 agtttttatt cttctgcctt taatttcttt tttgcacctt tagctaatcc tgcaaaaaat
     3061 tatattaact ggaagcaata cggatccaat aaaaataatg gtgtgttaat tgcaatacat
     3121 cctgaagctg caggcggagc acaaggtcag aaattcttaa atacctatga tgaaagcggg
     3181 caagaaatta tcagaaatca gtcagacttt ccggactttt atcataaaaa tggtaaaaat
     3241 gtagttaagg tttcaatatg gcggcagaaa caacgactgc gggtttatgt aaatgaaacc
     3301 aaaatttggg atattccaaa ggcattcaat gccacagcaa aatataattt tatgggattc
     3361 cgtacagatg gttatcataa tgaagaaaat gcttattttc tgagcaatat cagggttgct
     3421 gtaggtgcac ctgataccag acataaattt ttggaattgg gaaaatattc tacaacagga
     3481 attaaatttg acgtgaacag tgcaaccatt aaaggcgaat cttatggaac attaaaagac
     3541 ttcgcatctg taatgacaga aaacccggat gtgaaagtaa aaatagtggg gcatactgac
     3601 agcgacggtg atgatgctaa aaacttggaa ctgtctataa aaagagcaga agctgttaaa
     3661 agtgcattga ataaagaatt tggtattgac aacagccgca tggaaacaga aggcaaagga
     3721 gaaacccaac ctgcagataa taatacaacg ccagaaggca aagccaataa cagaagagta
     3781 gaat
//
LOCUS       DAOQII010000251         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_15110_length_3772_cov_6.688997, whole genome shotgun sequence.
ACCESSION   DAOQII010000251 DAOQII010000000
VERSION     DAOQII010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15110_length_3772_cov_6.688997"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..717
                     /locus_tag="PLF48_10365"
     CDS             202..717
                     /locus_tag="PLF48_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49774.1"
                     /translation="MIRKIIATQSLLLCSATFLFAQHTLVLKSGEKMTGTVQQIKDGN
                     ISFLFKGNPMTFKVSEASAIYFDAATETKADNTEMSADMRGVKYEMPGRTITKPPKIE
                     NLTMEKGVVVVEISIDKYGHVTKASPGAEGTTTTSSYLLTKAKQAAESVMFNNCPKCP
                     LDTKGTITLTF"
     gene            744..1643
                     /gene="miaA"
                     /locus_tag="PLF48_10370"
     CDS             744..1643
                     /gene="miaA"
                     /locus_tag="PLF48_10370"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNF49775.1"
                     /translation="MPKDKKLIIIAGPTASGKTQIAIEVAQLLKTEIVSFDSRQIFRE
                     MTIGTAVPDTQQLKAIKHHFIHSHSIAEDMNASRYANEATHTINQLLEKNNTVVVTGG
                     TGMYMHAFLFGLDATSNTDYQLRASLEKECAEKGLEYLAHQLKTLDKKSYHEIDIHNP
                     RRVIRALEIIYSSGKTRSSFIDETIKTPAYSFTLYVLNPDRQLLYENINNRVDLMIEK
                     GLVDEARQLLPYRHLTALHTVGYKELFEYFDRNSTLDQAIEKIKQHTRNYAKRQLTWF
                     RQYDDAIWITQEAMKKKIEEKSL"
     gene            complement(1640..3715)
                     /locus_tag="PLF48_10375"
     CDS             complement(1640..3715)
                     /locus_tag="PLF48_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNF49776.1"
                     /translation="MEVILATPYWYILLCLLAGLGCAWWLYGGNSSFNESPWLRRILF
                     FLRFLSVSVIAFLLLSPFIKSAIREVEKPAIAIAFDNTSSILLDSTHLSADAINQLAE
                     KLTSTFKNDYDANRYSFGSTVENEVTKNDFSEKSTNISAMFDYLKSLYAGRNLGAVIV
                     LSDGIYNKGSNPVYAAEKLKVPVYTIAVGDTAINRDLLISDVVYNKTAFLGNNFPVEI
                     GVTMRQAAGASVTLTVKEDSLTLFSRMITVSGNNYFTKIPVILSANKKGIHKYKIRLS
                     AIDGEKNLLNNEAEIYVDVSERKMKILLLAQSPHPDIAAIKSVLDKSNLYETKVLFQE
                     PTDENFGNYNLVVLQQIPSAKSATLLQKIKTAEVPYFIIAGAQMNIATFNNTGTGVTI
                     NQPVAKMNEVYAKVSDQFVLFSLSDNVQTVLSQLPPLYAPFGNYKMEKETNTLLTQKI
                     GNIQSSMPLWYFSSTGTMRVGVLLGEGLWRWQLNEYALSGNHDVTDELILKSVQYLTS
                     KEKKEPFMIQYKRSYTEGENVEFNATMYNAAGELINEPEVRLNIYNDEKKEFRYTFNR
                     SNNTYQLNAGMLSPGRYTFSAETKTGTKVYKETGEFSVVPLLLENFESTANHQLLNTI
                     SSNTGGIMVSADKINLLIDAIQKNETVKPVSYYHKKLIELISLKSLLIICLLLIATEW
                     FLRKRFGSY"
BASE COUNT         1248 a          737 c          672 g         1115 t
ORIGIN      
        1 aagcaagatt cctaatgctg ttttacgctg actttgatag gttactaatt tcataattct
       61 aatactttat ggtgccaaaa ttatcattct gtattgaaga tgcctaatat cgtttctatt
      121 gttgaaaact gttctcatat ttcaattcaa attcattctt acaacagcaa caaaaattta
      181 tttttgaaaa aagtttaaat tatgatcagg aaaattattg caacccagtc acttttgtta
      241 tgcagtgcta ctttcttatt tgcacaacat acccttgttt taaaaagcgg agaaaaaatg
      301 actggcactg tacagcagat taaagatgga aatatcagct ttttatttaa aggcaaccct
      361 atgactttta aagttagcga agcctctgca atatattttg atgcagcaac agaaacaaaa
      421 gcagataaca ctgagatgag tgccgacatg cgtggtgtaa aatatgaaat gcccggacgc
      481 actatcacca aaccgccaaa gattgaaaat cttaccatgg agaaaggtgt tgttgtagtg
      541 gagatttcta ttgacaagta tggccatgta accaaagcca gcccgggtgc agaaggaacc
      601 accactacaa gcagctattt actgactaaa gcaaagcagg ccgcagaaag tgttatgttt
      661 aacaactgcc ccaagtgtcc gttagatact aaaggcacta tcacgcttac cttttaactt
      721 tgccttaagc aaaggataaa aaattgccaa aggataaaaa gttaatcatc attgccgggc
      781 ctacagcatc aggcaaaaca caaattgcca ttgaagttgc ccaactgctg aaaacagaaa
      841 tcgtttcttt tgattcgagg caaatttttc gtgaaatgac tattggcact gccgtgcccg
      901 atacccaaca actcaaggca ataaaacatc acttcattca ttcgcactct attgcagaag
      961 acatgaatgc aagtcgctat gccaatgaag caacccacac catcaatcaa cttttagaaa
     1021 aaaacaatac tgttgttgtc acaggcggaa caggcatgta tatgcatgct tttctctttg
     1081 gtcttgatgc tacttcaaac accgactatc aacttagagc aagtctggaa aaagaatgcg
     1141 cagaaaaagg attggagtat ctggctcatc agctaaaaac cctcgacaaa aaaagttatc
     1201 atgaaataga cattcacaat ccacgcaggg tgatccgtgc cttagaaatt atttacagct
     1261 ccggaaaaac acgctcgtct tttattgatg aaacaatcaa aacaccagca tacagcttca
     1321 cactttatgt tttaaacccc gacagacaat tactttatga aaacatcaac aatcgtgtgg
     1381 atttgatgat agaaaaagga ctggttgacg aagccaggca actactaccc taccgccatc
     1441 ttaccgcatt gcatacagta ggctacaaag agttgtttga gtatttcgac agaaacagta
     1501 cacttgatca ggcaattgaa aaaataaaac aacatacacg caactatgcg aaaagacaat
     1561 taacatggtt caggcagtat gatgatgcca tttggattac acaagaagca atgaagaaga
     1621 aaattgagga gaaaagtctt taataagaac cgaaacgttt tcttaaaaac cactctgtgg
     1681 caatcaacaa caaacagatt ataagtaaac ttttcaagct tatcaactca atcagtttct
     1741 tatgataata cgatacaggt ttaactgttt cgtttttctg aatggcatca attaataaat
     1801 taattttatc ggcagaaacc attatacctc ctgtgtttga tgaaatggta ttgagcagtt
     1861 gatgattggc agtagattca aaattctcca gcaacaatgg taccacagaa aactctccag
     1921 tttctttata aaccttagtg cccgtttttg tttcagcact aaaggtataa cgtcccggtg
     1981 acaacattcc ggcattcaac tgataggtgt tgttgcttct gttgaaagta tatctaaact
     2041 cctttttctc atcgttataa atattcagtc gaacctccgg ttcattaatt aattcaccgg
     2101 ctgcattgta cattgtggcg ttaaactcaa cattctcacc ttcagtatag gagcgcttat
     2161 attgtatcat gaaaggctcc tttttttctt tcgaagtcag atactgaacc gactttaaaa
     2221 ttaattcatc tgtaacatca tgatttccgg agagtgcata ttcattcaac tgccagcgcc
     2281 acaatccttc gccaagcaaa acaccaacac gcatagttcc tgttgaagag aaataccaaa
     2341 gtggcattga cgattgtatg ttaccgattt tttgtgtcag caatgtattg gtctctttct
     2401 ccattttata atttccaaaa ggtgcatata atggcggtag ttgagacagc accgtttgca
     2461 cattgtcaga caagctgaac aacacaaact gatcgcttac tttagcatat acttcattca
     2521 tcttggcaac aggctgattg atggttacac ctgtgccggt attgttgaat gttgcaatat
     2581 tcatttgcgc accggcaatg ataaaataag gcacttctgc tgtcttaatt ttttgtaaaa
     2641 gtgtagcaga ctttgccgat ggaatctgtt gcaggactac aagattgtag ttgccaaagt
     2701 tttcatcagt tggctcctga aaaagcactt tggtttcata caaattactt ttatcaagta
     2761 cagattttat tgccgcaata tcaggatgcg gtgattgtgc cagaagcaat attttcattt
     2821 tgcgttcgga aacatcaaca taaatttctg cttcattatt aagcaaattc ttttcaccgt
     2881 caatagcaga tagtcgtatt ttatatttat gaataccttt tttattagca gaaaggatga
     2941 caggaatttt tgtaaaataa ttattgcctg atacagtaat cattctgctg aaaagtgtta
     3001 atgaatcttc tttaacggtt agtgtaacag atgctcccgc agcctgcctc atggtaacgc
     3061 ctatttcaac agggaaatta tttccaagaa aagccgtttt attgtacacc acatctgaaa
     3121 ttaacaaatc acgattgatg gctgtatcgc caacagcaat tgtataaaca ggtactttaa
     3181 gtttttctgc agcataaaca ggattgctgc ctttattata aattccatcg gacaatacaa
     3241 ttacagcacc aagatttcgt cctgcataaa gcgattttaa ataatcaaac atcgctgaaa
     3301 tgtttgtact cttttcagag aaatcatttt tggtaacttc gttttcaacc gtgctgccaa
     3361 aagaataacg attggcatca taatcgtttt taaatgttga agtaagcttt tctgccagtt
     3421 gatttattgc atctgctgac agatgtgttg aatcaagtaa tatggaagaa gtgttatcaa
     3481 atgcaatggc tatggcaggt ttttcaactt cgcgtatagc agattttata aaaggcgaca
     3541 ataacagaaa tgcaatgact gaaacggata aaaatctaag aaagaataat atacgcctca
     3601 gccagggtga ttcattaaag gacgaattgc ccccatagag ccaccatgca cagcccaggc
     3661 ctgccagcag acaaagcaaa atataccaat atggtgttgc aagtattact tccaaagcaa
     3721 aacgatgagg ggcaaatata atagtattgg aatgatactt attttagctg cg
//
LOCUS       DAOQII010000252         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_15591_length_3700_cov_4.912757, whole genome shotgun sequence.
ACCESSION   DAOQII010000252 DAOQII010000000
VERSION     DAOQII010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15591_length_3700_cov_4.912757"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="groEL"
                     /locus_tag="PLF48_10380"
     CDS             complement(<1..677)
                     /gene="groEL"
                     /locus_tag="PLF48_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNF49777.1"
                     /translation="MSSKLISYSTESRDKLKAGVDALANSVKVTLGPKGRNVIIEKKF
                     GAPAITKDGVTVAKEVELEDPFENMGAQLVRDVASKTNDVAGDGTTTATVLAQAIIAP
                     GLKSITAGANPMDLKRGIDKAVGAVVAELKSISEKVGKDFSKIKQVGTISANNDEEIG
                     QLIAQAMEKVTTEGVITVEEAKGTETYVDVVEGMQFDRGYISPYFVTNADNMECEMEK
                     PYILIYD"
     gene            complement(723..1007)
                     /locus_tag="PLF48_10385"
     CDS             complement(723..1007)
                     /locus_tag="PLF48_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNF49778.1"
                     /translation="MAKAKLNIQPLADRVIVKAAEAETKTKSGIIIPDSAKEKPQKGE
                     VMAVGSGKKDEPMTVKVGDTVLYGKYSGTEINIDGADYLIMRESDILAIV"
     gene            1187..2161
                     /locus_tag="PLF48_10390"
     CDS             1187..2161
                     /locus_tag="PLF48_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF49779.1"
                     /translation="MGYAKSFLGKELYEISYADVEYFFKDVKEESDKIEFKSFYSNDE
                     KHFNHKESGIIRAICGMLNSDGGLIIWGAPMGKSISGRKEKIYEGSLSPLEISIEKDA
                     FISKISNVISPSPKGIKFQRLEKNGKYVYLVEIPQSEYAPHQYNNVYWMRLDGQTRAA
                     PHHYIEALFKKITYPNLQGKLILDSVTTSESSYVQAKIKIEIANKSLFQNEHDIFIKL
                     IAENSFIVSNDFGKPVKDKGDKQGSEMIVEQAKSILYHLSPLRNYFSILLMKEKDENK
                     KFSFILIFGGKASPLKMSKYILKTGNELLKETTLQTEIENMYLHELNY"
     gene            complement(2179..2742)
                     /gene="pth"
                     /locus_tag="PLF48_10395"
     CDS             complement(2179..2742)
                     /gene="pth"
                     /locus_tag="PLF48_10395"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNF49780.1"
                     /translation="MKFLIAGLGNIGNEYQHTRHNIGFDMADAFAAKLGIPFELAKLA
                     IYGKGTFKGHQIHIIKPTTYMNLSGRAVKYYKTQLAIPTENCFVMVDDLALPFGQLRI
                     RTKGSSAGHNGLKSIEDELQTQEYPRLKFGLGNDFPRGRQVEFVLGKWTKDELEKLPE
                     RIQTATEIITSFCTAGIQHTMNLYNNK"
     gene            complement(2807..3169)
                     /gene="rplS"
                     /locus_tag="PLF48_10400"
     CDS             complement(2807..3169)
                     /gene="rplS"
                     /locus_tag="PLF48_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNF49781.1"
                     /translation="MHKVKEITEKFVTKKDVPVFKAGDNINVHYKIIEGNKERIQIFR
                     GDVIQRNGKGLTETFTVRKISNGVGVERIFPLHSPNIDKIEVNKIGKVRRAKIFYIRE
                     LKGKASRIKERISNSVKK"
     gene            complement(3226..>3700)
                     /locus_tag="PLF48_10405"
     CDS             complement(3226..>3700)
                     /locus_tag="PLF48_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNF49782.1"
                     /translation="CIEKLKEERSYDEIIFTTPDGELLNQKMVNSLSVKKNLIILCGH
                     YKGIDERLREHVITKEISIGSYVLSGGELPAAVLADAIGRLIPGVLNDETSALTDSFQ
                     DNLLSPPVYTRPSDYKGWKVPDVLLSGNDKLIDEWRYQQSLERTRKRRPDLLEND"
BASE COUNT         1115 a          736 c          609 g         1240 t
ORIGIN      
        1 ttatcgtaaa taagaatata cggtttttcc atttcgcatt ccatattatc tgcattggta
       61 acgaaatacg gagagatata acctctgtcg aactgcatac cctctactac atctacatac
      121 gtttctgtac ctttagcttc ttcaacagta attacacctt ctgtcgttac tttttccata
      181 gcttgtgcta tcaactgccc aatttcttca tcattattgg ctgaaatagt acctacttgt
      241 ttgattttgc tgaaatcttt tcccactttt tcagaaatag attttaactc tgcaactact
      301 gcaccaacag ctttatcaat accgcgtttc aaatccatag gattagcacc tgccgtaata
      361 gattttaagc ctggtgcaat gatagcctgc gccaaaacag tggcagttgt tgtaccgtca
      421 cctgcaacat cattggtttt agaagccaca tcacgcacta attgtgcacc catgttttca
      481 aacggatctt ccaactctac ttctttagca acggttactc cgtctttagt gatagccggt
      541 gctccgaatt ttttttcaat gataacattt cttcctttag gacctaatgt tactttcacg
      601 gagttagcta atgcatcaac gccggctttt aatttatctc ttgattctgt actgtaactg
      661 attaattttg aactcatttt ttatataatt tttaatgtta aatgattaat gttttcaagc
      721 cattatacga tagccaaaat gtcagattct ctcataatta agtaatctgc accatcgata
      781 ttgatttctg ttccgctgta ttttccgtac aataccgtat cacctacttt tacggtcatc
      841 ggttcatctt ttttgccgct gcctactgcc attacttctc ctttttgtgg tttttctttt
      901 gcagaatccg gaataatgat gcctgatttt gttttagttt ctgcttctgc cgctttcacg
      961 ataacgcggt cagccaatgg ttgaatgttt aacttagctt ttgccataat tatactgatt
     1021 ttgattaaaa aataagattt accctttttt tacgaattat atgccaattc aaaaaagcgg
     1081 acaaaaatac atgttttgat tttattcttt catttttagt tgtgtacaat agaaaaattg
     1141 acagttatta acctttaatt aattataaga atgcttacat tcgtatatgg gctatgccaa
     1201 atcatttctg ggcaaagaac tttacgaaat cagttatgct gacgttgaat actttttcaa
     1261 agatgtaaag gaagaatcgg ataaaataga attcaaatcc ttttattcta atgatgaaaa
     1321 gcattttaac cataaagaaa gtggcattat acgggctatt tgcggcatgc tcaactccga
     1381 tggtgggctg attatttggg gagcacccat gggcaaaagt atcagcggga gaaaagaaaa
     1441 aatttatgaa ggcagtttga gtccgcttga aatctctata gaaaaagatg catttatcag
     1501 taaaatcagc aatgtaattt cgccctcacc aaaaggtata aaatttcaac gcctggaaaa
     1561 aaacggcaaa tatgtttatc tcgttgaaat accgcaaagc gaatatgccc ctcatcagta
     1621 caacaacgta tactggatgc gtttagatgg acaaacccga gctgcaccgc atcattatat
     1681 tgaggcatta tttaagaaaa tcacctatcc caatttacaa ggaaaattaa ttttagattc
     1741 tgttaccaca tcagaaagca gctatgtgca ggcaaaaatt aaaattgaaa ttgccaacaa
     1801 aagcctgttt caaaatgagc atgatatctt tatcaaactg atagccgaaa acagcttcat
     1861 cgtcagtaat gattttggta agcctgtaaa agacaaaggt gataaacaag gcagcgagat
     1921 gattgtagag caggcaaagt ccattttata tcacctgtcg ccattacgca attatttctc
     1981 tatattattg atgaaagaaa aggacgaaaa caagaaattt tcgtttattc tcatatttgg
     2041 aggaaaagct tctccactga aaatgtcgaa atatatctta aaaaccggaa acgaattgct
     2101 caaagaaacc acacttcaga cagaaattga aaacatgtat ctgcatgaac tgaattatta
     2161 atctcatcaa actttctttt atttattatt ataaagattc atcgtatgct gaatacctgc
     2221 cgtgcagaaa gaggtaataa tttctgttgc tgtttgaatg cgttcaggta atttttctaa
     2281 ctcatcttta gtccattttc ccaatacaaa ttccacttgt cttccacgag ggaaatcatt
     2341 gcccaaccca aattttaaac gggggtattc ttgtgtttgc aattcatctt ctatactttt
     2401 caatccatta tgccctgcac tgctaccctt ggtacgtata cgcaattgcc cgaatggcaa
     2461 tgctaaatca tctaccatca cgaaacaatt ttcagtcgga atagctaatt gagttttata
     2521 atatttcact gccctgccac tcaaattcat atatgtcgta ggtttaataa tatgaatctg
     2581 gtgacctttg aatgtccctt ttccgtaaat ggccaatttg gctaactcga atggaatacc
     2641 aagcttagcg gcaaaagcat ctgccatatc gaaaccgata ttatgacgag tatgttgata
     2701 ttcgttgccg atattaccta agcctgcgat gagaaatttc ataaaatcaa aatttaataa
     2761 aaaatgccgc tacaagtgta acggcatttt attgtaataa taaatattat tttttaacag
     2821 aattagaaat gcgctcttta atacgagaag cttttccttt cagttcgcgg atatagaaaa
     2881 ttttagctcg acgaacttta ccgattttat ttacttctat cttgtcgata ttcggtgagt
     2941 gtaacgggaa aattctttct acaccaacac catttgaaat tttacgaacc gtaaacgttt
     3001 ctgtcagtcc ttttccgttt ctttggatta catctccgcg gaaaatctgg atacgttctt
     3061 tgtttccttc aataatctta taatgtacat tgatattgtc acctgctttg aaaacgggta
     3121 catctttttt ggtaacgaat ttttcggtta tttcttttac tttatgcatg tcaaaaaatt
     3181 tagcgtgtgc aaagataggg attttccttt aaaatgcaat attatctaat cgttttctaa
     3241 caaatccggt cttcttttgc gggttcgttc cagactttgc tgatatcgcc attcatctat
     3301 cagcttatcg ttgccactca ggagcacatc cggcaccttc cagcctttgt aatcagacgg
     3361 acgggtatat acgggtggag acaataaatt atcctgaaac gaatctgtaa gtgcggaagt
     3421 ttcatcattc aatacgcccg gaataagcct gccaatagca tctgctaaaa cggcagcggg
     3481 cagctcccca ccggaaagca catagctgcc aatggagatt tctttagtta tcacatgctc
     3541 tctcaatctt tcatcaatac ctttgtaatg tccgcatagg atgatcaggt tttttttaac
     3601 agaaagcgaa ttgaccatct tttggttaag cagttccccg tccggggtgg taaaaataat
     3661 ttcatcataa ctacgctctt ctttcagctt ttcaatacat
//
LOCUS       DAOQII010000253         3669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_15825_length_3669_cov_4.967072, whole genome shotgun sequence.
ACCESSION   DAOQII010000253 DAOQII010000000
VERSION     DAOQII010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15825_length_3669_cov_4.967072"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="PLF48_10410"
     CDS             <1..1226
                     /locus_tag="PLF48_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNF49783.1"
                     /translation="LEAITIVSIYYFVFLFFAAGIARGCQVIISRHNGEMAYHKIGIA
                     FDHLLVIGLVLSLFLFIFYHFFTPVLMPYLIKNPAIQTDAVNYLKGINWSTPFMVLGF
                     CYNGFYSGIGKTRIITWATIGMAITNAILGYLFIFGNFGLHPMGIKGAGISTGIANIV
                     LLLFYIVDFYKQEYAVQYHAYRFKNIQWDEIKSILQLSSPIVLQNTVGVASWCYFYIC
                     VERLGGNNLAVSGILKSLFIFLGIPIWSFSSTSNTVVSNIIGQGRTDAVVPALKKVLL
                     LAVITTGSSVLYVLLFPNFVFSLFTNDASILQDIIKPYHTLSIGMFFFMFAMVMNQSI
                     VGTGATFIPAFVEVVCSISYVIYCEYFIIHLHSPLYIAWGCEILYWMLLFTFTFVYWY
                     SGIWKKYIKHIDTLE"
     gene            1226..1969
                     /locus_tag="PLF48_10415"
     CDS             1226..1969
                     /locus_tag="PLF48_10415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF49784.1"
                     /translation="MEADSKMEWFATWFDSPLYHILYKNRNQEEANQFIDNMLRKLGI
                     EYGTMLDLACGKGRHAYYLAEKGFDVVGVDLSKSSIDYAKTMYKRDNLEFYVHDMRLP
                     FRINFFDYVFNFFTSIGYFNSLDENNKVFAAIHDGLKPKGKVLIDFMNAEKVMQHLVK
                     RENKVIDGYKFYLRRDLQDGKIIKNILVEKGDQVWIFKEEVQALQPHHFHQFLNNNKL
                     TLLQEFGDYELNPFHPKRSDRYIVLAQKL"
     gene            1972..2622
                     /locus_tag="PLF48_10420"
     CDS             1972..2622
                     /locus_tag="PLF48_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF49785.1"
                     /translation="MRKKVMVIIGAGEGISKATAELFGKQNYTICLIARKKDKLEKIT
                     NELRDKNIDAHYFIASVSDSDTIRDTFEQIWQLFPHIDILHFNVSKSKQVDIVSETSD
                     SLTRDFKTNVASVMTCLQLVLPDMEKIGEGTILLTGGGLALHPNYELGSLSIGKAGLR
                     NMAQSLHDSLKSKNIYVGTVTVCGFVAEDSPKHSPKIIAELFWKLHTERKDWEIIQ"
     gene            2624..3196
                     /locus_tag="PLF48_10425"
     CDS             2624..3196
                     /locus_tag="PLF48_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF49786.1"
                     /translation="MDTLLQLDFRLFHFINTDCSTFLLDVLCPILRDRMTWIPLYIML
                     GIWVMYKFKLEGLPMIIGVLLCFALADIISYQILKPLIHRLRPCHNSLVHARLVLGHC
                     GGKWSFPSSHASDHMAISMGIILFGIFKNKWVNGLMICWALTVGFSQIYVGVHYPTDI
                     LAGFLLGTGCAFAAKYILHLIGKRFFSWKQ"
     gene            3387..>3669
                     /locus_tag="PLF48_10430"
     CDS             3387..>3669
                     /locus_tag="PLF48_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49787.1"
                     /translation="MGPDALYGIVFVVRKQEIQQNGFGALGSQYALLAQQTFTDTGQL
                     AIPVLDNLSEEITVITGYASNAPSYMVLDDVRLSTYAEQTYLTQTFDEDI"
BASE COUNT         1085 a          649 c          752 g         1183 t
ORIGIN      
        1 ctctggaagc tattacaatt gtcagcatct actattttgt ttttttgttt tttgctgcag
       61 gaattgcgcg cggctgtcag gttatcatct ccaggcataa tggcgaaatg gcctatcaca
      121 aaatcggtat tgctttcgac catctgttag ttatcggatt ggttttgtct ttatttttgt
      181 tcatcttcta ccattttttc accccggtat taatgccata tctcatcaaa aatcctgcaa
      241 ttcagactga tgctgtcaat tatttgaaag gtatcaactg gtcaactcct tttatggtac
      301 tgggattttg ttataatggt ttttattcag gaattggcaa aacacgtatc atcacctggg
      361 ctacaattgg catggccatt accaatgcca tactgggcta tctgtttatt tttggtaatt
      421 ttggattaca tcccatgggt ataaaaggtg caggaatttc tacaggaatt gccaatattg
      481 tcttgttgct gttttatatc gttgactttt ataaacaaga atatgctgtt cagtatcatg
      541 cataccgttt taaaaatata caatgggatg aaattaaatc tattctgcaa ctttcatctc
      601 ctatcgtatt gcaaaataca gtgggtgtag catcttggtg ttatttttat atttgtgtgg
      661 aaagactggg aggaaacaat ctggcagttt caggtattct gaaaagctta tttatttttt
      721 taggtattcc gatatggagt ttttccagta catccaatac cgtagtgagt aatatcatag
      781 gacaaggcag aacagatgca gttgtgccgg ctctaaaaaa agtactctta ttagccgtaa
      841 ttactaccgg cagtagtgtt ttatatgtat tgttgtttcc caattttgtc ttttccttat
      901 tcactaatga tgccagtatt ttacaagata ttatcaagcc atatcataca ctcagcatag
      961 gcatgttctt ttttatgttt gccatggtga tgaatcaaag tatagttggt accggtgcca
     1021 ctttcattcc ggcattcgta gaagtagttt gcagtatatc gtatgttatt tattgcgaat
     1081 actttataat tcatcttcac tcaccattat acatagcctg gggttgcgaa atactctatt
     1141 ggatgctctt atttacattt acttttgtat attggtacag cggaatctgg aaaaaatata
     1201 ttaaacatat cgatacactc gaataatgga agcagatagt aaaatggaat ggtttgccac
     1261 gtggtttgat tcaccactgt atcacatttt atataaaaac cgcaatcagg aagaagccaa
     1321 tcaatttata gacaatatgc tccgcaaact gggtatagag tatggcacta tgctcgattt
     1381 agcttgtggc aaaggcagac atgcctatta tttggcagaa aaaggtttcg atgttgttgg
     1441 tgttgattta tcaaagtcga gcattgatta tgctaagacc atgtacaagc gtgataattt
     1501 ggagttttac gtacatgata tgcgcttgcc tttccgtatc aatttctttg attatgtctt
     1561 caattttttt accagtatcg gctatttcaa ttctttagat gaaaacaaca aagtatttgc
     1621 tgccattcac gacggtttga agcccaaagg caaagtattg atagatttta tgaatgccga
     1681 aaaagtaatg cagcatttag taaaacgtga aaataaggtg atagacggat ataaatttta
     1741 tttgcgaaga gatttgcaag atggtaaaat cattaaaaac attttagtgg aaaaaggcga
     1801 tcaggtctgg atatttaaag aagaagtgca ggcattacaa ccccatcatt tccaccaatt
     1861 tttaaacaat aataaactaa cattgttaca ggaattcgga gattatgaat tgaatccatt
     1921 tcatccgaaa cgctctgaca ggtatattgt attagctcaa aaattataag tatgcgaaaa
     1981 aaagtaatgg tcatcatagg cgcaggtgaa ggcatcagta aagccaccgc tgaattattc
     2041 ggcaagcaga attatacaat ttgtttaatc gccagaaaaa aggataagct cgaaaaaatc
     2101 acgaatgaac tccgagataa aaatatcgac gcacattatt ttattgccag cgtgagcgac
     2161 agtgatacga tacgtgatac gtttgaacaa atatggcagt tgtttccgca tattgatatt
     2221 cttcatttta atgtttctaa aagtaaacaa gtagatatcg taagtgaaac ttccgattcg
     2281 ctgaccagag attttaaaac gaatgtggca tctgttatga cctgtttaca gctggtattg
     2341 cccgacatgg aaaaaattgg agaaggtact atcctgttga ccggaggtgg gctggccttg
     2401 catcccaatt atgaattggg ttctttgtcg attggtaagg ccgggttgcg taatatggca
     2461 caatcactgc atgactcatt gaaatcaaaa aatatttatg taggcacagt tactgtatgc
     2521 ggatttgttg cagaagattc accaaaacat tcgcctaaaa tcatagccga attattctgg
     2581 aaattacata ctgaacgcaa agactgggaa attattcaat aaagtggata cccttttaca
     2641 gctcgatttt cgtctgtttc attttatcaa tacagattgc agtacatttc tactggacgt
     2701 actatgtccc attttacgag atagaatgac atggattcct ttatacatta tgctgggcat
     2761 ttgggtgatg tataagttta aattagaggg cttgcctatg ataattggag tgctgctgtg
     2821 ttttgcatta gccgacatca tttcatacca aatcttgaaa ccgctgattc accggttgcg
     2881 accctgtcat aattctctag tgcatgctcg tttagtatta gggcattgcg gtggcaaatg
     2941 gagttttccg tcctcacatg ccagtgacca catggctatc agcatgggta tcatcttatt
     3001 cggaatattt aaaaataaat gggtaaatgg cttaatgata tgttgggctt tgacagtagg
     3061 attttctcag atatatgtag gcgttcacta tccgacagat attttagccg gttttttact
     3121 tggaacaggg tgtgcttttg cagcaaaata tatcttgcat ttaattggga aaagattctt
     3181 cagctggaag caataaatac caaagctctc tccgttcgcc attagttggt gtttaggcat
     3241 tgcatagctt tagcgttgat acctcacctg ccgataaatt cccatttaga aatcgaaaaa
     3301 aagttatatc tttaaatgtg tcaagtaagt tgcaagatat agagtacgac agtcgacggg
     3361 tgataaaacc tgtcgtgagg ggtgaaatgg gacccgatgc cctgtatggc attgtcttcg
     3421 tggtgcgcaa gcaagagata cagcaaaacg gctttggcgc actgggcagc cagtatgcgc
     3481 tgcttgccca gcagacattt accgataccg gacagttggc tatcccggtg ttagacaacc
     3541 taagcgagga gataacagta ataacgggct atgcctctaa tgcgccatcg tatatggtgc
     3601 tggatgatgt gcgcctgagc acctacgcag agcaaaccta ccttacccaa acctttgacg
     3661 aggacatag
//
LOCUS       DAOQII010000254         3664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_15857_length_3664_cov_4.168468, whole genome shotgun sequence.
ACCESSION   DAOQII010000254 DAOQII010000000
VERSION     DAOQII010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15857_length_3664_cov_4.168468"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="sprA"
                     /locus_tag="PLF48_10435"
     CDS             complement(<1..1001)
                     /gene="sprA"
                     /locus_tag="PLF48_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNF49788.1"
                     /translation="MEFKNINNKNIAKVILCALLYLYADATAQDSIKLKYPLSDRNTD
                     FYTTQPKSSLDFKDPKIIEKKVEYDPATGKFIIYEKIGDTYYRTPTYMTYDEYLEYSK
                     REMENEYFQDRSRAIDLAERKSKQPFLYQGPELYNRFLGGTKIEIKPQGNVDVTIGVN
                     SQRIDNPILPQNQRRNINFDFDMNINLGLQASVGDKLKLGITYNTKSGFAFENQMKLG
                     YKGKEDDIIQALEFGNVSLPLRSALIKGPQNLFGMKTQFKFGRLLITNILSQQKSKTE
                     NIRIENGAQTKKFEIKSDEYEENKHFFFSQYFRDQYEPSLSRLPFVGAQTVINRIEV"
     gene            complement(1021..1614)
                     /gene="ruvA"
                     /locus_tag="PLF48_10440"
     CDS             complement(1021..1614)
                     /gene="ruvA"
                     /locus_tag="PLF48_10440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNF49789.1"
                     /translation="MFAYIQGKLSFKSPTHVYIECGGIAYILHISLSTYSQIEDKEQV
                     KLFTHLVVREDAHLLYGFFTEEEKTMFLHLISVSGIGPNTARLVLSSLSVNEFHQAII
                     REDVRLIQSIKGIGPKSAQRLILELKDKLKKENLTEILPALSGSSAIAHSAEALAALS
                     MLGFQKSHAEKAMQKAMKENPGLESVEEIIKASLKNL"
     gene            complement(1701..>3664)
                     /locus_tag="PLF48_10445"
     CDS             complement(1701..>3664)
                     /locus_tag="PLF48_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNF49790.1"
                     /translation="DPDPVVMSAQIAVRLKKIQKTIPLDYNEHVQKFITFNVNRKEHV
                     AKVLGRSKKYFPIYEPILAQYGVPDEMKYLSVVESALNPFATSPRGAAGPWQFMYSTA
                     QRFELDINKRFDERRDPYKSCEAACRYLKYMYNLYGNWQLAIASYNCGPGNVNKAIKK
                     AGGSKNFWDIRPYLPAETRAYVPMFIATVYAMKYASQYGIAPMELNRETTQRVTVKEK
                     ISLNDLVNLIGIPKQTIIDENPSLLTTLIPAGFTLNIPNSKYITFNKYQDSMYINALD
                     ILTAQKLDLPKVIKPESTPVETNCETEPVTDYSSKPITKQPASKEEPLASKPQPANAD
                     NEKSFFTKLKEKFTTKKEETKPAETKPKPKTQPLKDEPPAKVKVVKESDKKQDKTQTD
                     KHKNEQDKNKTIVDKKQTEAEKKKAEADKKQTETAKNNTQQPKSGMGKTATETSTNTT
                     LKTDILQDITKQKNYKLPDLIKEDDDNYKVITYTVKDGDNLGYIAHWFHCKVKDIRRW
                     NELNGNFIDVDDELLIYVHKNDYKKFTRFNYLSLRMKEMLSAEILTQEEIDAEKLKEQ
                     MTDSFSTVTNQQAKTQTPPKQTPVQQPVVAKQNCFEEYTIKQGENLWTVARKKGYGSV
                     KELMEWNNFKQTPTLHKGDVIKARKIACK"
BASE COUNT          932 a          657 c          699 g         1376 t
ORIGIN      
        1 catacttcaa ttcggtttat cacggtttgt gcacctacaa aaggcagtct actcaagctg
       61 ggttcatatt gatcacgaaa atattgagag aagaaaaagt gcttgttttc ttcatattca
      121 tccgatttta tttcaaattt cttggtttgt gcaccattct caatacggat attttctgtt
      181 tttgattttt gctgcgaaag gatattggta atcagtaaac gaccgaattt aaactgtgtt
      241 ttcatcccga ataaattctg cggccctttg atgagtgcac tgcgaagcgg aaggctgaca
      301 tttccaaatt ccagagcttg tatgatatca tcttcttttc ctttatagcc caacttcatt
      361 tggttttcaa atgcaaaacc cgatttggta ttataagtaa tgccgagttt cagtttgtcg
      421 cctacggatg cttgtaagcc gagattgata ttcatatcaa aatcaaaatt gatattgcgg
      481 cgttggtttt gcggcaaaat gggattgtct atacgttgcg aattaactcc aatagttaca
      541 tctacgtttc cctgtggttt tatttcaatt tttgttccgc ctaaaaatcg gttatacaat
      601 tcaggacctt gatataagaa aggttgcttg cttttgcgct cggcgaggtc tatggcacgg
      661 gaacgatcct gaaaatattc attttccatt tctctttttg aatattccaa atattcatca
      721 taagtcatgt aagtaggcgt gcggtaatac gtatcgccaa ttttttcata aatgataaat
      781 tttcctgtag cagggtcgta ctctactttt ttctcaataa ttttcgggtc tttgaaatcc
      841 agactgcttt ttggttgcgt tgtataaaaa tcggtattcc gatctgaaag tggatatttt
      901 agtttaatag aatcctgtgc tgttgcatca gcatataaat acaataaagc gcagagtatt
      961 actttggcaa tatttttatt atttatgttc ttaaattcca ctcaggacaa ttcttttagt
     1021 ttacaaattt tttaagctgg ctttaataat ttcttctaca gattccaaac ccggattttc
     1081 tttcattgcc ttttgcatgg ctttttcagc atggcttttc tggaagccaa gcatgctgag
     1141 tgcagcaagt gcttcggcac tatgtgcaat agctgatgaa cccgaaagtg caggcaggat
     1201 ttccgttaga ttttctttct tcaatttgtc ttttaattcc agtatgagcc gctgtgcaga
     1261 tttaggacca atacctttta ttgattgtat taaacgcaca tcttcccgga ttattgcctg
     1321 atgaaattca tttactgaca aagaagataa aaccagtctg gccgtatttg gaccaatccc
     1381 ggaaacagat atcagatgca ggaacatcgt tttttcttct tctgtaaaaa aaccatacaa
     1441 aagatgggca tcttcacgaa cgacaaggtg cgtgaaaagc ttaacttgtt ccttgtcttc
     1501 aatttgagaa taggtagata aactgatatg cagtatgtag gcaatacctc cacattctat
     1561 ataaacatgt gtgggtgatt taaatgatag ttttccctgt atataagcaa acataaataa
     1621 aaggtcaaaa gtaccaaaaa tctctggaat tgagatgatt aaagtatata atttgttcta
     1681 caagaaaata ggtacgtatt ttacttacaa gcaatttttc tggcttttat cacatcccct
     1741 ttgtgcaggg taggggtttg tttgaaatta ttccattcca tcagctcttt tacactgcca
     1801 tagccttttt tgcgggcaac cgtccagagg ttttcgcctt gttttatggt gtattcttca
     1861 aagcagttct gttttgcaac aacaggttgc tgcactggtg tttgttttgg aggtgtctgt
     1921 gtttttgctt gttgattagt tacagttgaa aacgagtctg tcatttgttc ttttaacttt
     1981 tccgcatcta tttcttcctg tgtcagtatt tcggcactca gcatttcttt catccgcaag
     2041 ctaagataat tgaatctggt aaatttctta taatcgtttt tgtgaacgta aattaacaat
     2101 tcatcgtcaa catcaataaa attgccattc agttcattcc atcttcttat atcttttact
     2161 ttacaatgaa accagtgtgc tatatacccc agattgtcgc catctttaac tgtataggtg
     2221 attactttat aattatcatc gtcttctttt attaaatcag gcagtttgta atttttttgc
     2281 ttggtaatat cttgtaaaat atctgtcttt aatgttgtgt tcgtacttgt ttcagttgca
     2341 gttttaccca taccagattt aggctgctgt gtattgtttt ttgctgtctc tgtttgtttt
     2401 ttatctgcct cggctttctt cttttcggcc tcagtttgtt tcttgtcaac tatggtttta
     2461 tttttatcct gctcattttt atgtttatct gtttgggttt tatcttgttt tttatcgctt
     2521 tcttttacca cttttacttt tgccggcggt tcatctttca gcggctgagt tttgggtttt
     2581 ggcttagttt ctgccggctt agtttcttct tttttagtgg tgaatttttc tttcaatttg
     2641 gtgaaaaaag atttctcatt atctgcattt gcaggttgtg gtttagaagc taaaggctct
     2701 tcttttgaag caggttgttt ggttatgggt tttgaagaat aatccgttac cggttcagtt
     2761 tcgcagttgg tctcgacagg ggtactttcc ggttttatta ctttaggtaa atctaatttt
     2821 tgagcagtca gtatatccag tgcattgata tacatggaat cctgatactt attgaatgtt
     2881 atatattttg aatttgggat attcaatgtg aaaccggctg ggatcaaggt ggttaataaa
     2941 gagggatttt catcaatgat ggtttgtttg ggtatgccga ttaaattgac aaggtcattg
     3001 agtgatattt tttctttcac tgtaacacgt tgggtcgttt ctctgttcag ttccattggt
     3061 gcaatgccat actgagatgc atatttcatg gcatatacgg tggcaataaa catgggcaca
     3121 taagctctgg tttctgccgg taaataaggt cgaatatccc agaagttttt agagcctcct
     3181 gcttttttga tggctttgtt tacattgccg ggtccacaat tatatgaggc tattgccaac
     3241 tgccagttgc catacaaatt gtacatgtat ttcaagtatc tgcatgctgc ctcacaagat
     3301 ttgtaaggat cacgacgctc atcaaatcgt ttgttgatat ccagctcgaa acgctgagca
     3361 gtactgtaca taaattgcca tggtcctgca gctccccgcg gggaggtagc aaatggatta
     3421 agtgcagact ccaccacaga aagatatttc atttcatcag gaacgccata ttgtgctaaa
     3481 atgggttcgt atatcggaaa gtatttttta gatcttccta atactttagc cacatgttct
     3541 tttcgattta cgttaaaggt gatgaatttc tgaacatgct cattataatc cagaggaata
     3601 gttttttgta tttttttaag gcgaactgcg atttgggcac tcatgaccac cggatccgga
     3661 tcat
//
LOCUS       DAOQII010000255         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_16051_length_3638_cov_4.251744, whole genome shotgun sequence.
ACCESSION   DAOQII010000255 DAOQII010000000
VERSION     DAOQII010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16051_length_3638_cov_4.251744"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLF48_10450"
     CDS             complement(<1..206)
                     /locus_tag="PLF48_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49791.1"
                     /translation="MRTVFKYTTIFCIIVMMRLLAQADTLNITRLISTPKINSQICGV
                     GTEGIADQLVFESDSNGVQTRSYY"
     gene            516..1916
                     /gene="fumC"
                     /locus_tag="PLF48_10455"
     CDS             516..1916
                     /gene="fumC"
                     /locus_tag="PLF48_10455"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNF49792.1"
                     /translation="MEYRIEKDTMGQVKVPANVYWGAQTQRSIENFQIAQKTDKMPIE
                     IIYAFAYLKKAAAITNYKAKVLDKQKCDIIGKVCDEILAGKLDDQFPLVIWQTGSGTQ
                     SNMNVNEVIAYRGHVLLGGKLTDEKKLIAPNDDVNKSQSSNDTFPTAMHIAAYKMLIE
                     RTIPGIKKLRNTLAKKSKEFAKIVKIGRTHFMDATPLTLGQEFSGYVSQLDHGLRAIE
                     HTLAHLSELALGGTAVGTGINTPKGYAKNVAKDIATITGFPFITAENKFEALAAHDAI
                     VEAHGALKTVAVALMKIGNDIRMLSSGPRCGIGEIHIPDNEPGSSIMPGKVNPTQCEA
                     MTMVAAQVMGNDVAINIGGSTGHFELNVFKPVIIYNFLHSARLIGDVCVSFNDKCAVG
                     IEPIYGKIKENLDNSLMLVTALNTKIGYYKAAEIAQTAHKQNKTLKQVAVELGYVTAK
                     QFDEWVKPEDMVGSLK"
     gene            complement(1971..2723)
                     /locus_tag="PLF48_10460"
     CDS             complement(1971..2723)
                     /locus_tag="PLF48_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF49793.1"
                     /translation="MKKILVVYLLIGLFLGSVSGQDSLQRKSVYKYRLGVDIPIGLIT
                     LGTGGTSFLLKKKKKALTLDEINALQPTDINRFDRSAIYHFNKGSKIASDVFEITSMV
                     SPALLFVDKKIRQDYKTVLPIWIETFALTTALTAFTKELAQRKRPFVYNPNAPLGDKM
                     SKDAKSSFFSGHTSITAASTFFIAKVYADYHPESRWKPLFWTGAAVIPAVTGLLRYGA
                     GKHYFTDVIVGMAIGATVGILVPHLHKRQLRK"
     gene            2738..>3638
                     /locus_tag="PLF48_10465"
     CDS             2738..>3638
                     /locus_tag="PLF48_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF49794.1"
                     /translation="MIKVVLFPSKIAKFTQAKHNKNLRNTLFICIFNLITALAFSGNT
                     IIKGQIKDIDTNKPIYGATINIKLTEGYEFFALTDTMGIYEIMISGEILEKDYDIQIS
                     ARAYNTVNGVLRVKKNTLGNFFMKLKNTVNKDTTAHPLLKDTAIHTSALDGYADNNLV
                     FLIDVSSSMNEQNRLPLLKSAFKILIDNFRPSDRITLITFSDGVKEILPPTSAQNKEL
                     ILAAINNLTYESSSNGGKALDFAYKSARSSFIENGNNKIILATDGMITSGEKDYKKIQ
                     KLIQNGSKQKIALTLFLFGNSSDY"
BASE COUNT         1247 a          679 c          646 g         1066 t
ORIGIN      
        1 ctatagtatg atcttgtttg cacaccattg gagtcggact caaataccag ctgatcagca
       61 attccttccg ttcccacacc acaaatttga gaattaattt ttggtgtaga aatcaaccgg
      121 gtgatgttta aagtatctgc ttgcgccaat aaccgcatca tgacaatgat gcaaaatata
      181 gtcgtgtatt taaaaaccgt tctcatggtt taccattgca aagctaaaat aaactgcatt
      241 tacagttact aacacatttt ttagattatt tatatcatcc gtctaataaa aacacaaaac
      301 aatctcattt tcaataaatt acatacaaaa ttgtaagaat agttacttta acacatagct
      361 atttttgttt atcatccctt ccatttaagt ttgagataat aaaaatcatg tttcttttgt
      421 cattttttta ggacttttgc agaatattct caagattaaa acaaaatatt acggcaattt
      481 ttttgaaaca acaaaacaac acataatttt tttatatgga atacagaatt gaaaaagaca
      541 caatgggaca agtaaaagtg cctgcaaatg tctattgggg cgctcagact caacgctcta
      601 tcgaaaactt tcaaattgca caaaaaacag acaaaatgcc tattgaaatc atttatgcat
      661 ttgcctatct gaaaaaagct gctgccatta ccaactataa agcgaaggta ttggataagc
      721 aaaagtgtga tatcattggc aaagtttgcg atgaaatttt agccggaaaa ctggacgacc
      781 aatttccact cgttatctgg caaacaggtt ccgggacaca gtcaaatatg aatgtaaacg
      841 aagttattgc ttatcgtggg catgtcttat tgggcggaaa acttactgac gagaagaaat
      901 taatcgctcc gaatgacgat gtcaataaat cgcagtcttc aaatgatact tttcctacag
      961 ccatgcatat tgcagcctat aaaatgctga tagaaagaac aatacccggc ataaaaaaac
     1021 tgcgcaatac actggctaaa aaatcaaaag aatttgccaa aatcgtaaaa attggccgca
     1081 cacatttcat ggatgcaaca cccttaactt taggtcaaga attttcaggt tatgtatctc
     1141 aactggatca tggactgaga gcaattgagc atactttagc tcatttgtct gagcttgctt
     1201 tgggaggcac agcagttggc accggaataa acacgccaaa aggatatgca aaaaatgtgg
     1261 ccaaagatat tgccacaatt acaggatttc catttattac tgcagaaaac aaatttgaag
     1321 cacttgccgc tcacgatgct atcgtagaag ctcatggcgc attgaaaacc gtagctgttg
     1381 cgctgatgaa gattggaaac gatattcgta tgttgtcttc agggcctcgc tgtggtatcg
     1441 gtgaaatcca tattccggac aacgaaccgg gttcttcgat tatgcccggc aaagtaaacc
     1501 ctacacaatg cgaagctatg actatggtag ctgctcaggt aatgggtaat gatgttgcca
     1561 taaatattgg tggcagtacc ggacattttg agctcaatgt ttttaaaccg gtgatcatct
     1621 acaatttcct gcacagtgcc cgtttgattg gagatgtgtg tgtttcattc aacgataaat
     1681 gtgcagttgg catcgaacct atatatggca aaatcaaaga aaatctggac aactctttga
     1741 tgttagttac cgcattaaat actaaaatag gttattataa agcagctgaa atagcacaaa
     1801 cagcacataa acaaaataag accttaaaac aagtggctgt agaattgggt tatgttacag
     1861 caaaacaatt tgatgaatgg gtaaaacctg aagatatggt gggtagctta aaataaggaa
     1921 cagatatatt cctacaatta agtgaagttg ataaatcagc ttcacttttt ttatttcctc
     1981 aattgtcttt tatgcagatg cggcactaat atccctactg ttgcgccgat tgccatacct
     2041 acaatcacat ccgtaaaata gtgtttacct gcaccatacc gcaataagcc ggttactgcc
     2101 ggaataactg ctgcacctgt ccagaataaa ggtttccatc tcgattcagg atgataatcg
     2161 gcatatactt tggctataaa aaacgtactc gccgcagtaa tactggtatg cccggagaaa
     2221 aaagatgatt ttgcatcttt gctcatcttg tcaccaaggg gtgcattcgg attataaaca
     2281 aaaggtcgct tgcgctgtgc cagttcttta gtaaatgctg taagtgcagt tgtcagtgca
     2341 aatgtttcta tccaaatggg caaaacggtt ttatagtctt gtcttatttt tttatcaacg
     2401 aataacagcg ccggacttac catggaggta atttcaaaaa catcactggc aattttactc
     2461 cctttattga aatgataaat ggcagatcgg tcaaaacgat ttatatctgt aggttgcagt
     2521 gcattgattt catccaaggt cagtgccttt tttttctttt tgagtaaaaa gctggtaccg
     2581 cccgtaccca aagtaatcaa accaatcggt atatcaactc ctaaccgata tttgtaaaca
     2641 ctcttgcgtt gcaatgaatc ttgccctgaa actgaaccca aaaacagacc aattaataaa
     2701 taaacgacta agattttttt catcctttct aaatattgtg ataaaagtag tattatttcc
     2761 gagtaaaata gctaaattca ctcaagcgaa gcacaacaaa aatttgagaa ataccctgtt
     2821 catatgcatt tttaatttaa taactgcact tgcatttagt ggaaacacca ttattaaagg
     2881 acaaatcaag gatattgaca caaataaacc catctatgga gccacgatta acataaaact
     2941 tacagaggga tatgaattct ttgcactcac agatacgatg ggcatttatg aaatcatgat
     3001 atcgggagaa attttagaaa aagattatga tatccaaatt tcagctagag catataatac
     3061 ggtaaacggc gttttaagag taaaaaaaaa tacgctgggt aattttttca tgaagttaaa
     3121 aaacacggta aataaggata ctacagctca ccctttacta aaagatacgg caatacatac
     3181 ttccgctcta gatggatatg ccgataataa tctggtcttc ttaattgatg tttcttcttc
     3241 tatgaatgaa caaaaccgat tgcccttgtt aaaatccgct tttaagattt tgattgataa
     3301 tttcagacct tccgaccgaa ttactttaat tactttttct gatggagtaa aagaaatatt
     3361 accgccaact tcagcacaaa ataaagaact gattcttgcc gctatcaaca acttaactta
     3421 tgaaagctca tcaaacggag gcaaggcact ggattttgct tataaatcag cgagaagcag
     3481 ttttattgaa aatggaaata acaaaattat tctggcaact gacggcatga ttacttcagg
     3541 ggaaaaagat tataaaaaaa tacaaaagct cattcagaac ggaagcaagc aaaagatagc
     3601 cctcacatta ttcttgtttg gcaactcaag tgattatg
//
LOCUS       DAOQII010000256         3617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_16206_length_3617_cov_5.288321, whole genome shotgun sequence.
ACCESSION   DAOQII010000256 DAOQII010000000
VERSION     DAOQII010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16206_length_3617_cov_5.288321"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PLF48_10470"
     CDS             <1..844
                     /locus_tag="PLF48_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF49795.1"
                     /translation="LLWGLTAILGKLVTVNGFMLVWYRTLFVCLILLFYSKLIPELKR
                     LSSRDYLIMFGIGSVLALHWIAWYTSVKIANASVALSCIALVSFFIAFIEPLFLKKPF
                     ELKNILLGLFVIPGILLINNGLQVSLHKGFFVGILAAFLAAIYSVLNKKYTQHINSNI
                     ITFVELLGGFIFMTATLPIYLKLNWGKFEFLTAFDFLLMLIMTVFCTLLSYYLYFTAL
                     KATNAFKTSLINNLEPVYGILLSILILHENKHLNAQFYLGTAILVAVVFADGLIKEKR
                     IKLR"
     gene            complement(831..1961)
                     /locus_tag="PLF48_10475"
     CDS             complement(831..1961)
                     /locus_tag="PLF48_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF49796.1"
                     /translation="MPLFDVYPLLAIELVQGKGSYVYDKNGTAYFDLYGGHAVISVGH
                     NHPDFVAAMQKQIQELIYYSNSVHLPLQTVLADKLCAVSSYPDYQLFLCNSGAEANEN
                     ALKLASFFNQRKKVIAFKGAFHGRTHGAVAITDAPKIKAPINNDAHVLFLELNDETAL
                     ENAFTANEISAVIIEPIQGVNGIYEANASFLQKIRSLCDNYQALFIADEIQCGYGRTG
                     KFFAHQWANVKPDIISMAKGMGNGFPIGGILVSPKIKAEYGMLGTTFGGAPLACSAAI
                     AVLDILINEQLMENAHAVGEHIKENLKHVPVIKQIRGRGLMLGIEFEFPVKEFRNTLV
                     EKHHILTGNANQSNTLRILPHLGISKDEINLLLDIIKLPSEV"
     gene            complement(1966..2469)
                     /locus_tag="PLF48_10480"
     CDS             complement(1966..2469)
                     /locus_tag="PLF48_10480"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNF49797.1"
                     /translation="MLIFLIGFMGSGKSYMARNLSPLLNYAYVDMDLAIEAKENKTIR
                     EIFEQHGEDYFRRKEHTFIQELAISDKLIVSTGGGTPCFYENMNAMNVKGLTVYLNRS
                     KEDIIMRLEKGKYKRPLIQHLTTEELSLFYDTKLQERVHFYKQAKIFAGDKTPEEVAE
                     LLANYIK"
     gene            complement(2719..3462)
                     /locus_tag="PLF48_10485"
     CDS             complement(2719..3462)
                     /locus_tag="PLF48_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF49798.1"
                     /translation="MSETVEINNPEVSIARTRLIQIKGGKVKYGQVLVLDNVDFEVSS
                     GDFIYIIGKTGTGKTSLLKILYGDLEFNDGEGTVCGYNLKTLKRDAVPLLRRRLGIVF
                     QDYKLLKDRTIYENLEYVLHATGWTDKQKIKSKIQEATAMVGLESKLNNFPGQLSGGE
                     QQRIGIARALLNSPEIIIADEPTGNLDFETTEEIMNLLYNIHKTTKTPVIIATHNYSL
                     IENYPGIIYMCAYQNINRDDSHNQQYTTI"
     gene            3528..>3617
                     /locus_tag="PLF48_10490"
     CDS             3528..>3617
                     /locus_tag="PLF48_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNF49799.1"
                     /translation="MLKNDLILKAARGETTERIPVWMMRQAGRI"
BASE COUNT         1072 a          662 c          619 g         1264 t
ORIGIN      
        1 tttgctctgg ggtttgactg caattttagg gaaattagta actgttaatg gttttatgct
       61 cgtttggtat agaacgcttt ttgtttgcct catattgcta ttttactcca aactgatacc
      121 agaattaaaa cgacttagca gccgggatta tcttatcatg tttggcattg gcagtgtgct
      181 ggcgctgcac tggattgcct ggtacacttc tgtgaaaata gcaaatgcgt cagttgcact
      241 aagctgtatt gcattagttt cattttttat agcatttatc gaaccattat ttctcaaaaa
      301 accatttgag ctaaaaaaca tattgctggg cttatttgtt attccgggta ttttattaat
      361 caataatggg ctacaagtca gtttgcacaa gggatttttt gttggtatac tggctgcatt
      421 tctggctgcc atttacagtg tactgaataa aaaatacaca cagcatatta acagcaatat
      481 catcactttt gtagaattac tgggcggttt tatttttatg acagcaacat tacccattta
      541 tctcaaactg aactggggaa aatttgaatt cttaactgca tttgattttt tattgatgct
      601 gattatgaca gttttttgca ccttgctttc ttactatctt tattttacgg cactcaaagc
      661 cacaaacgct tttaaaacct ctctgataaa taatttagag cctgtttatg gtatactttt
      721 atctattcta atcttacatg aaaataaaca tctgaatgct cagttttatt taggaacagc
      781 tatactagtg gctgtagttt ttgctgacgg acttattaaa gaaaaaagaa ttaaacttcg
      841 ctaggcaatt taatgatatc aagaagcaaa ttaatctcat ctttactgat acccaagtgt
      901 ggaagtatac gcaaggtatt ggactggttg gcattgcctg ttaaaatatg atgtttttct
      961 actaatgtat ttctaaattc ttttaccgga aattcaaatt cgatacccag cattaatcca
     1021 cgacctctta tttgtttgat aaccggaaca tgttttagat tttcctttat atgttcacca
     1081 actgcatgag cattttccat taattgctca ttaatcaata tatccaatac ggcaattgca
     1141 gcagagcagg cgaggggagc acctccaaaa gtagtaccca acataccata ttcggcttta
     1201 atttttggcg ataccaatat ccccccaatt ggaaaaccat tgcccatacc ttttgccata
     1261 ctgataatat ccggttttac atttgcccat tgatgggcga aaaattttcc cgttctgcca
     1321 tagccacatt gtatttcatc agcaataaac aaggcttgat aattatcaca caaactgcgt
     1381 attttttgta aaaatgaagc gttagcttca taaataccat ttactccctg aatgggttct
     1441 ataatgaccg cactaatttc atttgctgta aatgcatttt ccagtgcagt ttcatcattt
     1501 aattccagaa ataacacatg tgcgtcgttg tttattggtg ccttaatttt gggagcatct
     1561 gtaatggcaa ccgctccatg tgttctgccg tgaaaggcac ctttaaatgc aattaccttt
     1621 tttctctgat taaagaagga agccagttta agtgcatttt cattggcttc ggcaccgctg
     1681 ttacacaaaa ataactgata gtccggataa gaggaaacag cacaaagttt atctgctaaa
     1741 actgtctgta aaggcaaatg taccgaattg gaataataaa tcaattcctg gatttgtttt
     1801 tgcatggctg caacaaaatc aggatgattg tgtcctacag aaataaccgc atgaccgccg
     1861 tataaatcga aataggcagt accattttta tcatatacat aactgccctt tccctgcact
     1921 aattcaattg ctaataatgg ataaacatca aacaaaggca taattttatt ttatataatt
     1981 agctaataat tctgctactt cttccggtgt tttatctccc gcgaaaattt ttgcctgttt
     2041 ataaaagtgt actctttcct gaagtttggt atcataaaac aagctcaact cttctgttgt
     2101 cagatgctga atgagcggac gtttgtattt gcctttttcc aaacgcatga taatatcttc
     2161 tttacttcga ttgagataaa cggttagccc ctttacattc atagcattca tgttttcgta
     2221 gaaacaaggt gtgccgcctc ctgtgctgac tatcagctta tcagagatag ctagttcttg
     2281 aataaatgta tgttctttcc ttcggaaata gtcttcgcca tgttgttcaa aaatttcgcg
     2341 tatggtttta ttttctttag cctctatagc caagtccatg tctacataag catagtttag
     2401 cagtggagat aaatttctcg ccatgtaaga tttgccactg cccataaaac caattaagaa
     2461 aatcaacata accgaaatta aaaaaaaata gcaggaagat gattttaagc tacagaaata
     2521 acccaaagat aaaatgcaaa taatatgtaa ttaatagtat aatgataaat tatatttaaa
     2581 tatggtattg aatctttgcg taaggtttgt tttttgagaa gagtattatc tatgcttaat
     2641 tatttttaaa tatgtattta ttctctttaa atagtatctg gctcagctat gcagtcagac
     2701 acataactga aaatctttct atatagttgt atattgctga ttatgtgaat cgtcacgatt
     2761 gatattttga tatgcgcaca tgtatatgat gcccggataa ttttcgataa gactataatt
     2821 atgtgtggca atgatgacag gtgttttggt cgttttatga atgttgtaga gcaaattcat
     2881 aatttcttct gtggtttcaa agtcaagatt gccggtcggt tcgtctgcaa taattatttc
     2941 aggtgaattc aataaggctc tggcaatgcc gatgcgttgt tgttcaccac ctgacagctg
     3001 tcctggaaaa ttgttaagct tactttccaa acccaccata gccgtggctt cttgaatttt
     3061 gctttttatc ttttgtttgt cagtccatcc cgtagcatgc agcacatact ctaagttttc
     3121 gtatatcgtt cggtctttaa gcagtttata atcctgaaat acaataccca atcgtctgcg
     3181 caaaagcgga acggcatcac gttttaatgt tttcagatta tagccgcaaa ctgttccttc
     3241 accatcatta aattccaagt cgccgtataa tatttttaat aagctggttt ttccggtgcc
     3301 tgtttttcct ataatatata taaaatctcc tgatgaaact tcaaagtcga cattatccag
     3361 cacaagtact tgaccatatt ttacttttcc gcctttaatt tgaattaaac gagttcgggc
     3421 aatacttact tccggattgt tgatttctac tgtttctgac attattagat atttatggaa
     3481 taaagttaaa aagcaatata cgtcttgatt tgtgtatttt tgcccacatg ttgaaaaatg
     3541 atttaatctt aaaagcagca aggggcgaaa ctaccgagcg tatacctgtt tggatgatgc
     3601 ggcaagcagg cagaata
//
LOCUS       DAOQII010000257         3615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_16221_length_3615_cov_4.639888, whole genome shotgun sequence.
ACCESSION   DAOQII010000257 DAOQII010000000
VERSION     DAOQII010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16221_length_3615_cov_4.639888"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLF48_10495"
     CDS             <1..708
                     /locus_tag="PLF48_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49800.1"
                     /translation="DANIVVCYKDGKNDYSASDSIKIDYPASKAGLRNAFSYLQRVSG
                     SSTKFFCFINNHGGGYRASNGLYYGGAADTNGDEPSTDTKHYDEDIFYYNESTDIRDD
                     SLAAWVNSVTFGTGIFVLKPCFSGGLIYDLRGANRVIISSGTETQVTYGHSSGNYGEM
                     SHNFIAAISGIAPSTGASVNADLNSDGQISMYEAFIYLKNAEEWKTDEQPQYNDDGTG
                     TPTATPSSSGFGASVFL"
     gene            complement(764..2464)
                     /gene="gldG"
                     /locus_tag="PLF48_10500"
     CDS             complement(764..2464)
                     /gene="gldG"
                     /locus_tag="PLF48_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNF49801.1"
                     /translation="MSGNHYIKQSFKILAISLLTVLLINIVSSFFYKRFDLTEEKRYT
                     LTPATKNLLKNLNENVEVEVYLEGKNLPAGIKSLRNETKELLQEFRNISKGRVTYKFF
                     DINSIKDQKKREEFQMNLVNKGLRPTNLEVKSNSGYSENIVFPGALVKAGGNEIPVQI
                     LENQFSVGAQGSLNNSINFLEYKTANAIQKAIRKKRPKILFLQGHGELGVAPLEYLLK
                     DLNAQGFEIDKLELDKDKLLNNGVDILMVAKPNSAISDPEKFLIDQFVMQGGKTIWLL
                     DNIICDLDSFKLAPSIFSVPRDLNLDDIMFRYGVRINHNLVMDLYCNQIPIIESIGGN
                     PQPKLFPWVFYPIAVSKNNHPIVKNLDPVALKFTSSIDTLGNLGVHKTILLSSSDYSR
                     EQNTPFQIYLEGAKQRPDPSLFNKKNIPMGVLLEGNFQSLYKNQLTEDYRKLLDIQQV
                     TYKAESEKNKMIVIADGDIASNELDAKGVPLPLGYDKYSQKMYANKDFMLNAIEYMVD
                     DNNLITARNREIKMRLLDKAGLQNKKGLWQVITLVLPLILILFFGTVYNSRRKKKYVA
                     "
     gene            complement(2517..3245)
                     /gene="gldF"
                     /locus_tag="PLF48_10505"
     CDS             complement(2517..3245)
                     /gene="gldF"
                     /locus_tag="PLF48_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNF49802.1"
                     /translation="MTALLRKEINEFFSSLIAYISMAVFFIVLGLNLFVFEGNIFDSN
                     YATLDSFFTLTPWILMFLIPAITMRTFADEKQSGTIELLGTKPLTDTQIILGKYLAAL
                     LLWFIIFLPTFIYFIAVKNLSLAGAPIDSGATWGSYIGLFFLGAVFTAIGVFSSSISN
                     NQIVAFILGVFLCYLMYDAFYRLSALPVFSGRMDYIIQSIGLNAHYDAISRGVVDTRD
                     IVYFLSVIGLFLIGTRTSLESRKW"
     gene            complement(3249..>3615)
                     /locus_tag="PLF48_10510"
     CDS             complement(3249..>3615)
                     /locus_tag="PLF48_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNF49803.1"
                     /translation="KTILFSTHIMQEVQAVCDRVVIINKGKIVADATPENLQQTLSGN
                     VQVEIEFELNENAESLKKIEEVVHVQQLSNTHFILQGRESKKLRKNIFTYATQKNNAL
                     LTLKEENKSLEDVFHELTK"
BASE COUNT         1085 a          707 c          673 g         1150 t
ORIGIN      
        1 gatgctaata ttgttgtttg ttataaagac ggtaaaaatg actatagcgc atctgacagt
       61 attaaaatag actatccggc ctcaaaagca ggattgagaa atgcattttc gtatttgcaa
      121 agggtgtcag gcagtagcac taagttcttt tgttttatca ataatcacgg tggtggttat
      181 cgggcatcta atggtttgta ttacggcggt gctgctgata ccaacggtga tgaaccatca
      241 acagatacca aacactatga tgaagatatt ttctactata atgaatctac tgatatccgc
      301 gatgattctt tagctgcttg ggtgaattca gtgacttttg gaacgggaat ttttgtttta
      361 aaaccatgct tcagcggagg tttgatttat gacttaagag gggctaaccg tgtaattatt
      421 tcttccggta cagaaactca ggtaacatac ggacacagtt ccggtaacta cggcgagatg
      481 tcacacaatt ttattgctgc tatatcaggc attgctccaa gcacgggtgc cagcgtaaat
      541 gctgacttga acagcgacgg tcaaatatct atgtatgaag cgttcatcta tctgaaaaat
      601 gcagaagaat ggaaaaccga tgagcaacca caatataacg atgatggcac aggtacacca
      661 actgccactc catccagttc aggatttggt gcaagtgtgt tcttataaaa aacagagaag
      721 aatatctgaa aaaaaggcag ctcaaaagag ctgctttttt atattatgct acgtactttt
      781 ttttccttct gctgttataa actgttccga aaaacaagat taaaattaac ggtaatacaa
      841 gtgttatcac ttgccatagc cctttcttgt tttgcaaccc ggctttgtct aacaagcgca
      901 ttttaatctc gcggttgcgg gcggttatca ggttattatc atccaccata tactctatgg
      961 cattcagcat aaaatcttta ttggcataca ttttctgaga gtatttgtca taacccaatg
     1021 gcaacggaac gccttttgca tccagttcat ttgaggcaat atcaccatct gcaataacga
     1081 tcatcttgtt tttttcgctc tctgctttgt acgttacttg ttgtatgtct aacaatttgc
     1141 gatagtcttc agtaagttgg tttttatata atgactggaa atttccttca agcaaaacac
     1201 ccattggaat attcttctta ttaaacagcg aaggatcggg gcgttgctta gcgccttcca
     1261 gataaatttg aaatggagta ttttgctctc tgctataatc tgaagaagag agtaaaatag
     1321 ttttatgcac gccgaggttg cctaatgtat caatggagct ggtaaatttt aaagcaacag
     1381 gatccaggtt ttttacaata ggatgattgt tcttgctcac agcaatcgga taaaataccc
     1441 acggaaaaag tttgggttgc ggattgccgc caatggattc tataatcggg atttgattgc
     1501 aatataaatc catcactaaa ttgtgattga ttcgaacacc ataacggaac ataatatcat
     1561 caagattcaa atcacgaggg acggagaaga tgctgggtgc cagtttgaaa gaatccaaat
     1621 cgcaaatgat attatccagc agccagattg ttttaccgcc ttgcatcaca aactgatcaa
     1681 tcaaaaattt ctccggatcg ctgatagcag aattaggctt ggccaccatc aaaatatcta
     1741 cgccattatt aagcaattta tctttatcta attccagctt gtcaatttca aatccttgcg
     1801 cattcaggtc ttttaataaa tactccagcg gagcaacacc cagctcacca tgcccctgca
     1861 aaaacaaaat tttaggtcgt tttttacgga tagctttttg aatggcattg gctgttttgt
     1921 attctaagaa attaattgaa ttattgagtg agccttgcgc accaacggag aattggtttt
     1981 ctaaaatctg cacaggtatc tcattgccgc cggcctttac caatgccccc ggaaatacga
     2041 tattttcaga atagccggag tttgatttta cctccaggtt tgttggacgc aaacctttat
     2101 tgaccaaatt catctgaaac tcttcccgtt tcttttggtc tttaatagag ttgatatcga
     2161 aaaatttata ggtcactctt cctttagaaa tattgcgaaa ttcttgcagc aattccttgg
     2221 tttcgttgcg taagcttttt atgccggcag gcaagttttt accttccaga taaacttcta
     2281 cttctacatt ttcattcaga tttttcaaca gatttttggt tgccggagtc agcgtatatc
     2341 tcttttcttc tgttaagtca aatcgtttat aaaaaaaaga agacacaata tttataagca
     2401 gtacggtaag caaacttatc gccagaattt taaaagattg ttttatgtaa tgattgcctg
     2461 acattagtga tttgtgtttt tccccatttc ttgttctcga attatcttgt tatagcttac
     2521 catttccggc tttctaaact ggttcttgtt ccgattagaa acaatccaat cacacttaag
     2581 aaatatacaa tatctctggt atctactaca ccgcgactga tagcatcata atgcgcattc
     2641 aacccaatgg actgaatgat ataatccatt ctgcccgaaa acaccggaag ggcactaagg
     2701 cgataaaatg catcatacat cagatagcac aaaaacacac ccaggataaa tgccacaatc
     2761 tgattattgg aaatggagga agaaaatacg cctatggcag taaataccgc acctaaaaaa
     2821 aacaaaccga tataactgcc ccaagttgca ccgctgtcaa tgggtgctcc tgcaagactt
     2881 aagtttttta ccgcaatgaa ataaataaat gtaggaagaa aaattatgaa ccataaaagc
     2941 aacgctgcta aatattttcc caaaataatc tgggtatctg tcaaaggttt ggtgcccaat
     3001 agctcaattg tgccgctttg tttttcatcg gcgaaagtgc gcatggtgat tgccggtatc
     3061 aaaaacataa gaatccaggg ggtgagtgta aaaaacgaat ccagggtagc ataattgctg
     3121 tcgaaaatat tgccctcaaa aacaaataaa ttcaacccca gtacaataaa aaacactgcc
     3181 atgctgatat aggctatcag tgaggaaaaa aattcgttta tttcttttct taaaagtgcc
     3241 gtcatgcgtt attttgttaa ttcatggaag acatcttcca ggctcttgtt ttcttctttc
     3301 agggtgagca gtgcattgtt tttttgtgtt gcataagtga aaatattttt gcgcaacttt
     3361 ttgctttctc ttccctgtaa tataaaatgc gtattggata gctgctgcac atgtacaacc
     3421 tcttctattt ttttcagact ttctgcattt tcatttaatt caaactcaat ttccacttgt
     3481 acgttaccac tcaaagtctg ttgtagattt tcgggtgtag catctgctac aatttttcct
     3541 ttattaataa tcactactct gtcgcagaca gcctgtactt cctgcatgat atgtgtggag
     3601 aacaagatgg ttttt
//
LOCUS       DAOQII010000258         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_16524_length_3576_cov_4.733314, whole genome shotgun sequence.
ACCESSION   DAOQII010000258 DAOQII010000000
VERSION     DAOQII010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16524_length_3576_cov_4.733314"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLF48_10515"
     CDS             complement(<1..675)
                     /locus_tag="PLF48_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF49804.1"
                     /translation="MSNYKFETLQLHAGQEIDPTTGSRAVPIYQTTSYQFKDTEHAAN
                     LFGLREFGNIYTRLMNPTTDVFEKRVAALEGGVAALATASGQSAQFIALNNILQAGDN
                     FVSTSYLYGGTYNQFKVQFKRLGVEVRFADGDDPASFEKLINDKTKAIYLESIGNPRG
                     NVPDWDAFKKLSEKYDLPIIVDNTFGAAGYLFQPLKHGAHIVVQSATKWIGGHGTSIG
                     GVIVDGG"
     gene            complement(918..2168)
                     /locus_tag="PLF48_10520"
     CDS             complement(918..2168)
                     /locus_tag="PLF48_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNF49805.1"
                     /translation="MERFQGLIGIVVILGIAFLFSNNKKKINLRLVGSGLLLQTIIAI
                     CIFKISVVRNFFQSLGKIMTHIEAFAHEGAGFVYGGLMATNYAGGSIPYSTPGVFIFG
                     FSVLATIILVCVLVAILYQYGIMQLIVSGIAKAMNYIMRVSGAEALSNVASAFVGQVE
                     AQVMIRPYLQTMTNSELLASMSGSLACIAGGILVVYVSFGAKAEYLLAASIMAAPGAL
                     VISKIVFPETEESQTMGNVKLEIKKPYQNIIDAISHGASDGMKISINVIAMLIGFIAL
                     IAMVNYILGKISYFLGMPFTLSLDWIFGKLFYPIAWCMGVPGSDVDTVATLMGQKLCI
                     NEFVAFSNMTHAGLTPKSEKGLLIASIAICGFANFSSVGMQIGGIGELAPGRRADLAK
                     LGMKALLCGTLASYLSATIAGILM"
     gene            complement(2457..>3576)
                     /locus_tag="PLF48_10525"
     CDS             complement(2457..>3576)
                     /locus_tag="PLF48_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNF49806.1"
                     /translation="LTLCYSPGVAEPVKQIANDEEKVWDLTIKNNTVAVVSDGSAILG
                     LGNLGAEAAIPVMEGKAMLFKKFAGIDAFPICLATQNTEDIIQTIRNISPVFGGINLE
                     DISSPRCFEIEERLQTLGIPVFHDDQHGTAIVVLAGLVNACKLLDKKIEDLKIVINGA
                     GAAGLAISKLLLCHEVDKNYCTPVKQIIMCDTKGIIHKDRADLNDFKREILKYSNPHN
                     MKGTLKDAMIGADVFIGVSQGNVITTEDVMTMSKVPVIFALANPNPEISPEKAYKGGA
                     GVVGTGRSDIPNQINNVLAFPGIFKGALEMRARRITPRMKVAAAYAIADCVPTAELNR
                     DNIVPSTLDMQVARRVADAVKLAYEDEMKNMETPKHAL"
BASE COUNT         1096 a          758 c          615 g         1107 t
ORIGIN      
        1 tccgccgtca acgattacac cgccgattga agtgccatgt cctcctatcc attttgtggc
       61 agactgtacc acgatatgcg caccatgttt cagcggttga aataaataac ctgctgctcc
      121 gaatgtattg tcaacgatga tgggtaaatc gtatttttcg gataattttt taaaggcatc
      181 ccaatcaggt acattgccgc gcggatttcc gattgattct aaataaatgg cttttgtttt
      241 atcgttgatt agtttttcaa aagatgcagg atcatctcca tcagcaaagc gcacttctac
      301 acccaagcgt ttgaactgta ctttaaactg attataagta ccgccgtata agtaagaagt
      361 agaaacaaaa ttatctccgg cttgcaaaat attattgagt gcaataaatt gtgccgactg
      421 tccggatgcg gtagccagtg cagccacacc accctctagt gcagccactc ttttttcaaa
      481 aacatccgta gtcggattca tcaaacgcgt atagatattg ccaaactcac gcaaaccaaa
      541 aaggttggct gcatgttcgg tatctttgaa ctgatacgaa gtggtttggt aaatgggcac
      601 ggctcttgac ccggtagtag ggtcaatttc ttgtccggcg tggagttgca gtgtttcaaa
      661 tttgtagttg ctcatattta tttgttttaa gtgatgaata aaaaaaatcc ttccgctttg
      721 ggaaggatat atgtttaagc ttattttgat tattgaaaaa taattaccct tccgattttt
      781 ttcggcaaca acagcaacat ggcatattga aataagtaat cataaaaatc aaagatagta
      841 aactattaaa atgttcgttt aaatttaatg ttatcaataa acaggtggct ccgtgggtaa
      901 aaatgctact gataacatta catcaaaata cctgctatgg tagcagaaag ataagaagcc
      961 aaagtaccgc acaaaagtgc tttcatgcct aatttagcta agtctgctct tctgccgggt
     1021 gccaactccc ctattccacc aatttgcata cccacagaac tgaaattggc aaatccacaa
     1081 atagcaatac tagctatcag taagcctttt tctgattttg gagttaaccc tgcatgagtc
     1141 atattggaaa atgcgacaaa ttcattgata caaagttttt gtcccattaa tgttgcaacg
     1201 gtatctacat cactacccgg aactcccata caccatgcaa taggataaaa caattttcca
     1261 aaaatccaat ctaaagataa ggtaaaaggc atgcctaaaa aataacttat cttacctaag
     1321 atataattta ccatggcaat taaagctata aaaccaatca acatcgcaat cacatttata
     1381 gatattttca taccatcact tgctccgtga ctaatagcat ctataatatt ttgatatggt
     1441 tttttgattt ctaatttcac attgcccatc gtttggcttt cttcagtttc aggaaataca
     1501 atttttgaaa taacaagtgc tccaggtgca gccataatac tggctgccaa taaatactcg
     1561 gcttttgcac caaaacttac ataaaccact aaaatacctc ctgctataca agccaaactg
     1621 cccgacatgg aagccagtaa ttcactgttt gtcatagttt gcaaataggg gcgtatcata
     1681 acttgtgctt ctacttgccc tacaaatgca ctcgccacat tactcaatgc ttctgcaccg
     1741 gaaacacgca taatataatt catagctttg gcaatccctg aaactattaa ttgcattatg
     1801 ccgtactgat ataatattgc tactaaaacg caaaccaaaa tgatcgtagc cagcacacta
     1861 aaaccgaata taaacacacc gggtgtacta taaggaattg aacctcctgc gtaattcgtt
     1921 gccattaatc cgccataaac aaagcccgct ccttcatggg caaatgcttc aatatgtgtc
     1981 attattttac ccaatgactg aaagaaattt ctgactactg aaattttaaa aatgcaaatg
     2041 gcaataatgg tttgtaataa caagccactt cctacaagtc tgagattaat tttctttttg
     2101 ttgtttgaaa ataaaaatgc aatgcctaaa attacgacaa tgccaatgag tccttgaaaa
     2161 cgctccataa atgttactgt gttttaacaa atataaaggt attttttgct tttttcgttt
     2221 ttttgaaaat aatgtgatta gtagcttgtt ttgacaaaaa taaatgctat aaatttattt
     2281 agtcaggtcg gaagacctga ctacactgga agacccgact ttctgtgtcg tcataagttt
     2341 ccttctgata aatagctgtc aatgaaaaga cttggattta ttgggatatt tcagacagtt
     2401 cggaagacct gactacactg gaagatctga ctaacttgaa aaacatacat gtttatttac
     2461 aaggcatgtt ttggggtttc catgtttttc atttcatctt cataagcgag ttttacagca
     2521 tcggctacgc gtcttgcaac ctgcatatcc aatgtagatg gtacgatatt atcgcggttc
     2581 aattctgctg ttggcacgca atcggcaatg gcatacgcag cagctacctt cattcttggc
     2641 gttattcttc gtgcccgcat ttcgagtgca cctttaaaga ttccgggaaa tgccagtaca
     2701 ttattgattt gattgggtat atcacttctt cccgttccta ccacacctgc gccaccttta
     2761 taggcttttt ccggagagat ttccgggttt ggattagcta aagcaaaaat aacaggaact
     2821 ttactcatgg tcataacatc ttctgtcgta atcacattac cctgactaac tccaataaaa
     2881 acatccgcac ctatcatggc atctttcaac gtacctttca tgttatgcgg attgctgtat
     2941 ttcagaattt cacgcttaaa atcattcaaa tctgcacggt ctttgtgtat aataccctta
     3001 gtatcgcaca tgataatttg tttcaccggt gtgcagtaat ttttatctac ttcgtggcaa
     3061 agcagcaatt tggaaattgc cagtccggca gctccggcac cgtttatgac aattttgaga
     3121 tcttcaattt ttttgtccag taatttacaa gcattgacca aacccgccaa tactactatg
     3181 gccgttccat gttggtcgtc gtgaaaaaca ggaataccca atgtttgcaa tcgttcttca
     3241 atttcaaaac aacgaggcga tgaaatatct tcgagattaa ttcctccaaa aacgggagaa
     3301 atattacgaa tggtttggat gatatcttct gtattttgtg tagctaaaca aatcggaaag
     3361 gcatcgatgc cggcaaattt tttaaacagc attgccttgc cttccattac gggaatagct
     3421 gcttctgctc ccaaattccc taagcccaaa attgcagaac catccgatac tacagcaaca
     3481 gtattgtttt taatagtcaa atcccaaact ttttcttcat cgttggcaat ctgctttacc
     3541 ggctcagcta ctcccggcga ataacataag gtcaaa
//
LOCUS       DAOQII010000259         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_16677_length_3554_cov_4.445842, whole genome shotgun sequence.
ACCESSION   DAOQII010000259 DAOQII010000000
VERSION     DAOQII010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16677_length_3554_cov_4.445842"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="rplI"
                     /locus_tag="PLF48_10530"
     CDS             complement(<1..455)
                     /gene="rplI"
                     /locus_tag="PLF48_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNF49807.1"
                     /translation="MELILLKDIENLGSKFDIVSVKPGYGRNFLIPKGFAKIANVSNM
                     KHLEEIKKQQSAKLAKLMEEWQAVAEKMKATKITVGAKAGATGKLFGSVTNIQLADAI
                     HKQMDIKIERKKIHILGEVKELGLYKANVNLFKEISTEILQRLKFNKPR"
     gene            complement(461..685)
                     /gene="rpsR"
                     /locus_tag="PLF48_10535"
     CDS             complement(461..685)
                     /gene="rpsR"
                     /locus_tag="PLF48_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNF49808.1"
                     /translation="MGQQKKKYCRFKKMGIKYVDYKDPQFLLKFVNDQGKMLPRRLSG
                     NSLKFQRKVSQAIKKARHLALMPYVTDLLK"
     gene            complement(729..1082)
                     /gene="rpsF"
                     /locus_tag="PLF48_10540"
     CDS             complement(729..1082)
                     /gene="rpsF"
                     /locus_tag="PLF48_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNF49809.1"
                     /translation="MSNYETVFITTPVLSNDEYTRTVKKYTDFIEKNGGKLVHKEDWG
                     IKQLAYPIQKKTTGFYTLLQFEADPQIIRKYEVEFKRDENIMRFLTTKLDKYALAYAG
                     QRREKLSGKTQKAEA"
     gene            1324..2919
                     /locus_tag="PLF48_10545"
     CDS             1324..2919
                     /locus_tag="PLF48_10545"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNF49810.1"
                     /translation="MAKFIFVTGGVSSSLGKGILAASLAKLLQSRGLNVTIQKFDPYI
                     NVDPGTMNPYEHGECYVTEDGAETDLDLGHYERFLGVHTSQANNVTTGKIYQEVINRE
                     RRGDYLGKTVQVIPHITDEIKRRVLLLGKTDNYDIILTEIGGTVGDIESLPFVEAIRQ
                     LKWQLPKHDYLVIHLTLVPYLSSAKELKTKPTQHSVKELQSEGVNPDIIACRAEISLG
                     SERKRKIAEFCNVDYDSVIEALDAECIYDVPINLQKERLDEIVLQKFGYEDMPKADLE
                     EWNSFLHRYKNPKKEITIGLVGKYIELKDAYISIYESFIHAGVFNECKVNVERIHAEE
                     ITKRNVREILGNMDGIFVAPGFGQRGIDGKIDTIRYARENNIPFFGVCLGMQMAVIEF
                     ARNVLHLENANSTEMQNSTPHPVIDMMKEQKTVVNMGGTMRLGAYRCTLKENTLAYKI
                     YEKEEISERHRHRYEFNNKYQSDFENNGMIISGVNPDTGLAEIIEIPEHKFFIGVQFH
                     PEFKSTILKPHPLFCHFVKACLS"
     gene            2952..>3554
                     /locus_tag="PLF48_10550"
     CDS             2952..>3554
                     /locus_tag="PLF48_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF49811.1"
                     /translation="MNICIVTGSGGLIGSESVEFFADKFDLIVGIDNDLRAYFFGNDA
                     STSWNKNRLEERFSNYKHYNIDIRNNADLEKVFAEYNKDIKLIVHTAAQPSHDWAARE
                     PFTDFTVNANGTLNMLEYTRLHCPEAVFIFTSTNKVYGDNPNYLPLVELETRWEIDES
                     HPYYKEGIDEYMSLDHTKHSIFGASKVAADIMCQEYGKYFN"
BASE COUNT         1107 a          607 c          674 g         1166 t
ORIGIN      
        1 aagcgaggtt tattaaactt taatctttgc aatatttcag tagaaatttc tttgaataaa
       61 ttgacattgg ctttatacag acctaattct tttacttccc ctaaaatatg gattttctta
      121 cgctcaattt taatatccat ttgcttgtga attgcatcag ccagctgaat attagtaacg
      181 ctaccaaaca attttccggt tgctcctgct ttagctccaa ctgtaatttt agtagctttc
      241 attttctccg ctacagcttg ccattcttcc ataagcttgg ccagctttgc agattgttgt
      301 ttcttaattt cttccaaatg tttcatattc gaaacatttg cgattttggc aaatcctttc
      361 ggtatcaaga aatttctgcc gtagcccggt tttacggata caatgtcaaa cttgctgcct
      421 aaattttcta tatcttttaa taaaatgagt tccatggcgt ttattttaaa aggtcagtaa
      481 cataaggcat caaagctaaa tgacgcgctt tcttaattgc ttgagatact ttgcgctgaa
      541 atttcaatga gtttccactc aatcgacgag gtaacatttt accttggtcg tttacaaatt
      601 tcaacaagaa ttgtgggtct ttatagtcca catatttgat gcccattttt ttgaaacggc
      661 agtatttttt cttttgttga ccgattttcg gtgcattcaa aaatttaatt tcgtcttgtg
      721 atgccatctt atgcctccgc tttttgagtt tttccagata atttttctct tctttgtcct
      781 gcatacgcca aagcgtattt atccagtttc gtagttagaa aacgcatgat gttttcatcg
      841 cgcttaaatt ctacttcata cttgcggata atctgaggat ctgcctcaaa ctgcaataag
      901 gtataaaaac ctgttgtttt cttttgaatg ggataagcca gctgcttgat gccccaatct
      961 tctttgtgaa ccagctttcc accgtttttc tcaatgaaat cggtgtactt ctttacggtt
     1021 cttgtgtact catcgttaga gagtacgggc gtggtaataa aaaccgtttc gtaattgctc
     1081 ataatgttta aaaaattgtt taaaaatgag gtgcaaagat agtaattatt cagaaaaaca
     1141 acgcatttga aacaaagata attacttaat cgtgttattt ttatgttttc tgtctcatta
     1201 taaatgacat aatctataag aggcttgtca aatccatatt aagaataaaa attcagttat
     1261 ctaaaaattt ctttatttga tgattgcgct tatctttaca gtccataacg cacttattaa
     1321 gttatggcta aattcatctt tgttacaggc ggggtttcat cttctttagg aaaaggaatt
     1381 cttgcagcat ctcttgctaa gttattgcaa tccagagggt taaatgttac tatccagaaa
     1441 ttcgacccgt atattaatgt cgatccgggc acaatgaatc cgtatgaaca tggagaatgc
     1501 tatgtaaccg aagatggagc agaaacagat ttggatttag gccattacga gcgtttcttg
     1561 ggtgttcaca cttctcaagc taataatgtt acaaccggaa aaatctatca ggaagttatc
     1621 aatagagagc ggagaggaga ttatttagga aaaacggtgc aagttatccc gcatatcact
     1681 gacgaaatta agcgacgtgt tttactgttg ggaaaaacgg ataattatga tataatcctt
     1741 acagaaatcg ggggaacagt gggcgatatt gagtcgttgc cattcgtaga agctattcgt
     1801 caattgaaat ggcaattacc gaaacatgac tatttggtca tacacttgac tctggtgcct
     1861 tatttgtcat ctgctaaaga gctcaaaaca aaaccaacac aacatagcgt gaaagagttg
     1921 caatctgaag gtgtaaatcc tgacataatt gcttgtcgtg ctgaaatttc attaggttcg
     1981 gaacgcaaac gaaaaattgc tgagttttgt aatgtagatt acgattctgt aattgaagct
     2041 ttagatgcag agtgtattta tgatgttccg attaatttac aaaaagaacg cttagatgaa
     2101 attgttcttc aaaaattcgg atatgaagat atgcccaaag ctgatttgga agaatggaac
     2161 tcttttctgc atcgctataa gaatcccaaa aaagaaatta caattggttt agttggaaaa
     2221 tatatagaac tgaaggatgc ttatatttca atttatgaat cttttatcca tgcaggtgtc
     2281 tttaatgaat gcaaagtaaa tgtagaacgc attcatgcag aagaaatcac gaaaagaaat
     2341 gtcagggaaa ttctcggaaa tatggatggt atttttgtgg ctcctggctt tggtcagcgt
     2401 ggcatagacg gcaaaatcga caccatacgt tatgctcgcg aaaataatat accgtttttt
     2461 ggtgtttgtt taggtatgca aatggctgtt attgagtttg ccagaaatgt attgcatctg
     2521 gaaaatgcta attctacgga gatgcaaaat agcacaccgc accctgttat tgatatgatg
     2581 aaagaacaaa aaacggtggt gaatatgggt ggcactatgc gcctcggggc ttaccggtgt
     2641 acattgaaag aaaatacact tgcttataag atttatgaaa aggaggaaat atctgaaaga
     2701 catcggcatc gttacgaatt caacaacaaa tatcaaagtg attttgaaaa taatggcatg
     2761 ataatatcgg gggttaatcc ggatacagga ttagctgaaa tcatagaaat acccgaacac
     2821 aaattcttca ttggtgtcca gtttcatcct gaatttaaaa gtactatctt aaagccacat
     2881 ccgctatttt gtcattttgt aaaagcctgc ttatcctgat ttttagtatc tttgcaggct
     2941 aaaaaattaa aatgaatatt tgtatcgtta ccggttcagg tggattaatc gggagtgagt
     3001 ctgtcgagtt ttttgcagac aaatttgatt tgatagtagg catagataat gatttacgag
     3061 catatttttt cgggaatgat gcctctacat catggaacaa aaaccgtttg gaagaaagat
     3121 tttccaacta taagcattac aatattgata ttcgcaataa tgcagatctg gaaaaagtat
     3181 ttgcagaata caacaaagat attaagctga ttgtccatac tgccgctcaa ccatcacatg
     3241 actgggccgc tcgagagcct tttacggact ttacggtgaa tgcaaacggt acgcttaaca
     3301 tgctggaata tacaagatta cattgtcctg aagccgtttt tattttcaca tcaactaata
     3361 aagtttatgg cgacaatccg aactatttac cattagtaga actggaaaca agatgggaaa
     3421 ttgacgagtc acatccttat tataaagaag gtattgatga gtatatgagt ttagaccata
     3481 ccaaacattc cattttcggt gcatcaaagg tagccgcaga tatcatgtgc caggagtatg
     3541 gcaaatattt taat
//
LOCUS       DAOQII010000260         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_16746_length_3544_cov_4.192892, whole genome shotgun sequence.
ACCESSION   DAOQII010000260 DAOQII010000000
VERSION     DAOQII010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16746_length_3544_cov_4.192892"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLF48_10555"
     CDS             <1..519
                     /locus_tag="PLF48_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49812.1"
                     /translation="KKPKEAKAKDAMIFAQQYFDMDSMQLALNGDGTHLGLTSIIKQY
                     GGTAAGNRAKFCAGIANLKLGKYAEAVKSLEDFSTSDELLNARKFGGIGDAYAEQKQM
                     DKAIDNYKKAADAADNELTAPSYLFRAALALEQKGSKKEALELYKKLFADYPNSQEGS
                     IAEMYIAKLEAK"
     gene            555..1031
                     /gene="ribH"
                     /locus_tag="PLF48_10560"
     CDS             555..1031
                     /gene="ribH"
                     /locus_tag="PLF48_10560"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNF49813.1"
                     /translation="MSSILQNLSDYNEHAVPSGKGKSIGIAVAEWHKDITFELLRGCV
                     ATLEKFDVQTDSIFITYVPGTFELPFAAKRLAETHHLSSVICLGCVIKGETDHDIYIN
                     QSVAFALQQLNLQYEIPFIFGVLTPNTEEQAKDRAGGKHGNKGVEAAITALKMGRI"
     gene            1059..1997
                     /locus_tag="PLF48_10565"
     CDS             1059..1997
                     /locus_tag="PLF48_10565"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNF49814.1"
                     /translation="MKVFYGLDNLPEFNHAVLTIGTYDGVHFGHQQIIKRLNELATNI
                     HGESILLTFDPHPRLILNPDENKLKLISTIDEKIELLASFGLQNLVIAEFSKTFSDMP
                     AEDYVRDILVAKFHPQYIVVGHDHRFGKNRTGDIYLLEKLAMKYGYQVEELPAQSIDE
                     IAVSSTKVRNFLLEGNIKEANQLLLHPFSLTGKVIHGDKVGRILGFPTANIDISNKYK
                     LIPASGVYAVKVDLENQTYFGALSIGYRESVFENSKLTIEVFILDYDGDLYNKELKMV
                     FVDYLRPQIKYENWELLKIQIEKDVEKIRELIFKSN"
     gene            2072..>3544
                     /gene="purH"
                     /locus_tag="PLF48_10570"
     CDS             2072..>3544
                     /gene="purH"
                     /locus_tag="PLF48_10570"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNF49815.1"
                     /translation="MKKIKSALVSVYYKDGLDVLIHALAKNDVTFYSTGGTQAFIEGL
                     GYTVIPVEKLTSYPSILGGRVKTLHPKVFGGILARRENEQDLAQLQEYAIPEIDLVIV
                     DLYPFEETVKNTKNEDEIIEKIDIGGISLIRAAAKNYKHTMIVSQKSQYAELAAYLEE
                     NNGETSHDYRRKYAENAFAVSSHYDTLIYAYFAEKNPFDYLKISYLHNDKLRYGENPH
                     QHGFYYGDLQSVFEILNGKQLSYNNLVDADAAIRLIAEFNETTFVVIKHTNSCGVASK
                     SNLTEAWLGALAGDPVSAFGGVLACNAKIDKLTAEELDKLFFEILIAPDYEADALDIL
                     IKKKNRIILKQKQAAFPKVQFKSILNGVIAQDYDIKKISADDLKCVTELTPNEEQITD
                     LLFANKIVKHLKSNTIVLAKGKQLLGMGCGQTSRVDALLQAIHKAQSFGFDLHGSVMA
                     SDAFFPFPDCVEIANKAGIKAVIQPGGSIKDNDSITYCNEN"
BASE COUNT         1192 a          640 c          711 g         1001 t
ORIGIN      
        1 aaaaaaccga aagaagccaa ggctaaagat gccatgattt ttgcacagca atattttgat
       61 atggactcta tgcaattggc attaaacggt gacggtacac atttaggact gacttccatc
      121 ataaaacaat atggaggaac agctgcagga aatcgcgcta aattttgtgc aggcattgcc
      181 aatctgaagc tgggaaaata tgccgaagca gtaaaatcac tggaagactt cagcactagt
      241 gatgaattgc tgaatgcacg taagttcggc ggaattggag atgcttatgc agagcaaaaa
      301 caaatggaca aagcgattga caattataaa aaagccgcag atgcagccga taatgaatta
      361 acagcaccta gttacttgtt cagagcagcg ttggccttag aacaaaaggg cagtaaaaaa
      421 gaagcactgg aattatacaa aaagctgttt gccgattatc cgaattctca ggaaggcagt
      481 attgccgaaa tgtatattgc caagttggaa gcgaaataat ctgaatttgt atctctccaa
      541 atcgcacttc atttatgtcg tctatattac aaaatttatc cgattataat gagcatgccg
      601 taccttccgg aaaaggcaaa tcaatcggta ttgctgtggc ggaatggcat aaagatatta
      661 cctttgaact cctgagaggt tgtgtggcaa cgctggaaaa atttgatgtt caaaccgact
      721 ctatttttat cacttatgta cccggtactt tcgaattacc atttgctgct aaacgacttg
      781 cagaaacgca ccatttaagc agtgtgattt gtttgggttg tgtgataaaa ggcgaaactg
      841 accatgatat ctatatcaac cagtcggtag catttgccct ccagcaattg aatttgcaat
      901 acgaaattcc gtttatcttc ggtgtgctta cgccaaatac agaagagcaa gcaaaagacc
      961 gtgccggcgg caaacatggc aacaagggcg tagaagccgc tattacagca ctaaaaatgg
     1021 gcagaattta atttgtttcc atttttcatt ataaaggcat gaaggtattt tatggattag
     1081 acaatcttcc ggaatttaat catgccgtac tcaccattgg cacttatgat ggcgtgcatt
     1141 ttggccatca gcaaataata aaaaggctaa acgaacttgc cacaaatatt catggcgaaa
     1201 gtatcttact gacttttgat cctcatccca gattgatatt aaatccggac gaaaataagc
     1261 ttaaattaat ctctacaatt gatgaaaaaa ttgagctgct ggcttccttc gggttgcaga
     1321 atctggtcat cgcagaattt tccaaaacct tttcggatat gcctgctgaa gattatgtcc
     1381 gcgatatatt agtggctaaa tttcatcccc aatatattgt agtaggtcac gatcaccgct
     1441 ttggaaaaaa ccgcacggga gacatttatc tgctcgaaaa attagcgatg aaatatggat
     1501 atcaagtcga agaactgcct gcacagtcaa ttgatgaaat tgcggtgagt tctactaagg
     1561 tgcgaaattt tttgctggaa ggaaacataa aagaagccaa ccaattgctt ttacatcctt
     1621 tcagcttgac cggaaaagtg atacatggtg ataaagtagg cagaatttta gggtttccaa
     1681 ctgccaatat tgatatcagc aataaatata aattgattcc ggcttcagga gtatatgccg
     1741 ttaaagttga cctcgagaat cagacctatt ttggtgcgtt gagtatcggc tatagggaaa
     1801 gtgtatttga aaacagcaaa ctcaccatag aggtctttat tttagattat gacggtgatt
     1861 tgtataataa agaattgaaa atggtattcg tggattatct gcgtccacag ataaaatatg
     1921 aaaactggga actgcttaaa atacaaattg aaaaagatgt ggaaaaaatc cgagaactaa
     1981 tattcaaaag caattagttt tatctcattt tttacattaa tttgatttac aaatcagctc
     2041 gtttagccta actttgtgca aacaatttcc tgtgaaaaaa attaaatctg ccttagtttc
     2101 cgtttattat aaagacggat tggatgtact cattcatgca cttgcaaaaa acgacgtaac
     2161 cttttattct actggcggaa cccaagcatt tattgagggt ttgggctata ctgttattcc
     2221 ggtagaaaag ctgacatcat atccaagcat attaggaggt cgagtaaaaa ctttgcatcc
     2281 taaagtattt ggaggcatac tggcaagacg tgaaaatgag caagatttgg cacaattgca
     2341 agaatatgct attcccgaaa tcgatttggt tattgtcgat ttatatcctt ttgaagaaac
     2401 cgtaaaaaac acaaaaaacg aagatgaaat cattgaaaag attgacatcg gaggcatctc
     2461 tttaattcgc gctgcagcaa aaaactataa gcacacgatg attgtttcgc aaaagtcgca
     2521 atatgctgaa ttagcagctt atctcgaaga gaataatgga gaaaccagcc atgactacag
     2581 acgcaaatat gctgaaaatg catttgccgt ttcctcacat tatgacacgc tgatttacgc
     2641 ttattttgca gaaaaaaatc cattcgatta cttaaaaatt agttacctcc ataacgacaa
     2701 acttcgatac ggggaaaatc cgcatcagca cggcttctat tatggcgatt tacagtcagt
     2761 ttttgaaata ctcaatggca aacaactttc ttacaataat ttggtagatg ctgatgctgc
     2821 aatacgatta attgctgaat ttaacgaaac cacctttgtg gtgattaaac ataccaattc
     2881 atgcggggta gccagtaagt ccaatttaac tgaagcttgg cttggtgctt tagccggaga
     2941 tccggtttct gcttttgggg gtgttttagc ctgtaatgct aagatagata aattgactgc
     3001 cgaagaattg gataagctct tttttgaaat actgatcgca ccggattatg aagcagatgc
     3061 attggacata ttgataaaga agaaaaacag aattattctg aaacaaaaac aagctgcatt
     3121 tcccaaagtg caattcaaat ccattttgaa tggtgtaatt gcacaagatt atgatattaa
     3181 aaaaataagt gctgatgact taaaatgtgt aacagaatta acaccaaacg aggaacaaat
     3241 caccgattta ttatttgcca ataagattgt caaacatctg aaatcaaata cgattgtact
     3301 ggcaaaaggc aagcaattat taggcatggg gtgcggacaa acctctcgtg tagatgcact
     3361 actgcaagct attcacaaag cacaatcttt tggttttgat ttgcatggaa gcgtcatggc
     3421 atcagatgcc tttttcccgt ttccggattg tgtggaaatc gcaaacaaag caggcataaa
     3481 agccgtcata caacccggag gttccataaa agacaatgat tctattacct attgcaatga
     3541 aaac
//
LOCUS       DAOQII010000261         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_17114_length_3499_cov_4.903891, whole genome shotgun sequence.
ACCESSION   DAOQII010000261 DAOQII010000000
VERSION     DAOQII010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17114_length_3499_cov_4.903891"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLF48_10575"
     CDS             <1..442
                     /locus_tag="PLF48_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF49816.1"
                     /translation="NIKAILEFTPLKKSEQKEKLESLLEEFGLTHVRKSMGKLLSGGE
                     RRRTEIARALASDPKFILLDEPFAGIDPIAVEDIQHIVEKLKYKNIGILITDHNVHET
                     LSITDRAYLLFEGKIIKQGNAEELAEDEQVRRVYLGQNFELRKK"
     gene            573..1238
                     /locus_tag="PLF48_10580"
     CDS             573..1238
                     /locus_tag="PLF48_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49817.1"
                     /translation="MKKLLNKILLLAVAATLSFVSCQKQNAAITTDTLDVKLANDQVF
                     KNVLSAASDLGVQLDVQSLSDENNITELSAIAARINAKTATTADYTRLEQITGVSYDH
                     FMGLLQNFGVALNELNKKYPQLSSMKQADLTSTFTKAIQSNTDLQNMLVNPAGVTERL
                     SACPLRDLCNLAVSLTRMFAGDAICTAISVSTIPVIGGLLCTLILNLGVSVLTAICNA
                     LPC"
     gene            1385..2569
                     /locus_tag="PLF48_10585"
     CDS             1385..2569
                     /locus_tag="PLF48_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF49818.1"
                     /translation="MKIKHFFFALSTLIAVTACKKSEITDNTVTYPAVEAAFGSNINL
                     NSLENYANQSIPAYIRKDNTGTNAITNAKATLGRVLFYDKNLSIDNTISCASCHKQEF
                     AFSDTALASSGVASGLTGRHSMRLVNARFGVENKFFWDERAATLEIQTTKPIQDFNEM
                     GFSGTNGRQGIADLLTKLSGISYYTELFKFVYGDTQITEARMQECMAQFVRSIQSFDS
                     KYDAGRSQVGNDGQPFPNFTAQENNGKQLFLTPPLFDSVGNRTAGGLGCNGCHNAPEF
                     DIDPNAGNNGIIGKLNAAGFDLTNTRAPSLRNLTNVSGNPNSPMMHTGVITTLQTVIG
                     HYGNINANSQNTNLDVRLKPNGFGQQLHLTATEVNAVIAFLKTLSGNDVYTNKKWSDP
                     FK"
     gene            complement(2576..3382)
                     /locus_tag="PLF48_10590"
     CDS             complement(2576..3382)
                     /locus_tag="PLF48_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF49819.1"
                     /translation="MAKSFLEKYGKWAIITGASSGIGVEFAHQLAAKGLHIILVARRR
                     QLLEKVAHEIEAKYQVQTKIIESDLSIDGFQHDILESVKDLEIGLLVNNAGMNCEGDF
                     YRGSLERNLMMIQLNVKAHYVLAFEFGKQFISRGRGGIIFTASTSAFNAHPYLSHYAA
                     TKAYILSLAESMNYEFKDKNVDVLALCPGMTESEMTKGMKKSAILMPAQPVVESALNQ
                     LGKSSSVVPGLINKTQVFFNTRILNRDGAKLLNGAILKKVLPGVSKKKQK"
BASE COUNT         1147 a          633 c          668 g         1051 t
ORIGIN      
        1 taatatcaaa gccatactgg aatttacacc cttaaaaaaa tcagaacaaa aagaaaaact
       61 ggaatccctt cttgaagaat tcggcttgac tcatgtgcgt aaatccatgg ggaaattatt
      121 gtcgggtggg gagaggagac gaactgaaat tgccagagca ctggcatccg atcctaagtt
      181 tattttgctg gatgagcctt ttgcaggcat cgacccgatt gctgtggaag acattcaaca
      241 tatagttgaa aaattaaaat ataaaaatat tggtatttta attaccgatc acaatgtgca
      301 tgaaacactg tctataaccg acagagccta tttgttgttt gaaggaaaaa ttatcaagca
      361 aggaaatgca gaagagctgg cagaagatga acaagtaaga cgtgtttatc ttggacaaaa
      421 ttttgaattg cgcaaaaagt aattttatca tatcccactt ttttacacta ctcaattatc
      481 ttaagataag aaaagtaagt gaattgtaaa tagcatatta caaattagta agatgtttca
      541 actatttgta actttattaa aatacctttt taatgaaaaa attattgaat aaaatcttgt
      601 tgcttgctgt tgctgcaaca ctttcttttg tttcttgtca gaaacaaaat gctgcaatta
      661 ccactgatac tttagatgtt aagttggcaa acgatcaagt ttttaaaaat gtactgagtg
      721 ctgcatccga tttgggtgtg caactggatg tgcaatcgct gagtgatgaa aataatatta
      781 ctgaactaag cgctattgcc gctcgtatta atgccaaaac agctacaaca gctgactata
      841 ctcgtttgga gcaaataacc ggtgtatctt atgatcattt tatgggttta ttgcaaaact
      901 ttggagtggc attaaacgag ttgaataaaa aatatccaca gttaagctct atgaaacaag
      961 cagacttgac gagtacgttt acaaaagcaa tccaatctaa tacagatttg caaaacatgt
     1021 tggttaatcc ggcaggagtg acagagcgtc ttagtgcttg tccgcttaga gatttatgca
     1081 atttagcagt atcgctaacc agaatgtttg ccggagatgc catttgtact gccatcagtg
     1141 tttctactat tccggtaata ggcggtttgc tttgtacact aatattgaat ttgggagttt
     1201 ccgttctgac tgcaatatgc aatgcattgc catgctaata aaatgaacaa ttcaaaaact
     1261 gtaaatatca gaggctgaat tttcagcctc ttttttttat cttgtaaaaa ttaatgtcaa
     1321 aaaaaatgga tctgattaaa cctttgaaat tttgaatagt ccaatgcaga aataacacac
     1381 atttatgaaa attaaacact ttttctttgc actaagtacc ttgattgctg ttacggcatg
     1441 taaaaaatca gaaataactg acaacactgt tacctatcca gccgtggaag cagcattcgg
     1501 ctcgaatatc aatttgaaca gtctggaaaa ttatgccaat caatctatac ctgcctatat
     1561 aagaaaagac aatacaggca ctaatgcaat tacaaacgca aaggcaacat taggcagagt
     1621 gcttttttac gataaaaacc taagtattga taatacgata tcttgtgcaa gttgtcataa
     1681 gcaagagttt gcttttagtg atacggcatt agccagtagc ggtgttgcaa gtggactgac
     1741 aggacgccat tcgatgcgat tggtgaatgc tcgttttggc gtagaaaata aattcttttg
     1801 ggatgagcga gctgctacat tggaaattca gacaactaag ccaattcagg attttaatga
     1861 aatgggtttc agtggtacta atggccgaca aggaatagct gatttattga caaaattatc
     1921 cggaatcagc tattatacag aactgtttaa gtttgtttat ggtgatacac aaatcacgga
     1981 agctaggatg caagaatgta tggcgcaatt tgtaagaagt atacagtctt tcgattctaa
     2041 atatgatgcc ggtcgttctc aggtgggcaa tgacggacaa ccttttccca attttacagc
     2101 acaggaaaat aacggtaaac aactgttttt gacaccaccg ttatttgatt ctgtaggcaa
     2161 cagaacggca ggcgggttgg gctgtaacgg ctgtcataat gcgccggaat ttgatattga
     2221 tcccaatgca ggcaataatg gaattatcgg taaacttaat gctgccggct ttgacttaac
     2281 caataccaga gcaccatcac tccgcaatct gactaatgtc agcggaaacc ccaattcgcc
     2341 gatgatgcat acaggtgtga ttactacgtt gcaaacagtt atagggcact atggaaatat
     2401 caatgccaat tctcaaaata caaatttgga tgtccgttta aagccgaatg gctttggaca
     2461 gcaattgcat ttaacagcta ctgaagtaaa tgcagttatt gccttcttaa aaaccttaag
     2521 cggcaacgat gtgtatacta ataaaaaatg gagtgacccg tttaaataaa ttcggctatt
     2581 tttgcttctt cttgctaaca ccgggcaata cttttttcaa aatagccccg ttcaataatt
     2641 ttgcaccatc acgatttaag atacgcgtat tgaaaaaaac ttgtgtttta ttaattaaac
     2701 cgggaactac ggaagagctt ttgcccaatt gatttaatgc actttccacc actggctgag
     2761 caggcatcag aatggcactc tttttcatgc ctttagtcat ttcagattct gtcatgcccg
     2821 ggcaaagtgc caatacatcc acgtttttat ctttaaattc gtaattcatg ctttcagcca
     2881 aagagagaat ataggccttg gtagccgcat agtgagataa gtaaggatgt gcattaaatg
     2941 cagatgtgga agctgtaaat ataattccgc ctctgccccg gctgataaat tgtttgccga
     3001 attcaaaagc cagtacataa tgagctttga cattcagttg tatcatcata agattgcgct
     3061 caaggctgcc acggtaaaaa tcgccctcgc agttcatccc ggcattattc actaataagc
     3121 ctatttctaa atcttttacg gattcaagaa tatcatgctg gaaaccgtct atactcaaat
     3181 cagattcaat tattttggtt tgaacctgat attttgcttc aatttcatgg gcaactttct
     3241 ccagtaattg ccggcgacgc gctactagaa taatatgtaa gcctttagca gccagctgat
     3301 gggcaaattc tacaccaata ccactggaag cgccagtaat aattgcccat ttgccatatt
     3361 tttctaaaaa tgatttagcc ataaaaatga attttcatac aagataaaaa aagcaatttg
     3421 caaagccaaa aattaaaact tttttcaact taagttaagt gttttcgtaa atgcgaattg
     3481 atgaaataaa gatttagag
//
LOCUS       DAOQII010000262         3450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_17524_length_3450_cov_4.348748, whole genome shotgun sequence.
ACCESSION   DAOQII010000262 DAOQII010000000
VERSION     DAOQII010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17524_length_3450_cov_4.348748"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLF48_10595"
     CDS             complement(<1..505)
                     /locus_tag="PLF48_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49820.1"
                     /translation="MKKHVLIYTLSILLAHTVSAYTLSNDYGFLENITFNMYNENVPP
                     YQVPAHIQSLEETLMFDILHATWKDKKWVAYTLNPQLLGTFKVGQPALLDNGSTKKMY
                     FVAGLPNSVGGLDLYVSEFKNNKWSKPENLGSKINTVYNESNPGLLNDHILTYSSNGI
                     IKKMDLNN"
     gene            675..1586
                     /locus_tag="PLF48_10600"
     CDS             675..1586
                     /locus_tag="PLF48_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49821.1"
                     /translation="MAVLNMNRLYRNIKCIVLTSVFCLVFVSQVAALGEVKIAGARQS
                     GMGFSSIGLVSVYSAYNNQAAGAFLEHPGVGLYYAPVFIGEGVSNISGVVAVPVKKAG
                     TFGFSVNYFGYSLYNEKKFGISYAIKLAKYLSIGMQLDYLNAKISGYGSKNYATFELG
                     FFSKPIDELAIALHVYNPLKLYVDKNTGEKIPSVLRLGITYEARKKFFISTQIDKDFT
                     QKGVVFRAGVEYTLKDLISFRAGVASSPVTGSFGIGVSIRQGVTIDAAFSYMSTLGFQ
                     PHFGFIYELKKKTKVGGTTSKKKSKKK"
     gene            complement(1605..>3450)
                     /locus_tag="PLF48_10605"
     CDS             complement(1605..>3450)
                     /locus_tag="PLF48_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNF49822.1"
                     /translation="KITKMFLIGLGKADITAFERGSGMLGYGLYTHVALDVETPLYAR
                     AYIIEDLDNQHKVAIVNCELCFITPHLKQEVVKNLQKHAPELKINDQNLMLCAQHTHC
                     SPAGYSAHAIYNISVPGYIAHITKTIVDGVTEALIEANKNKTKADISIGKSSFEDDTP
                     VSFNRTVNSYNQNPEVKKYSFSQRHLAVDREMTLLQFVAEDGRLLGSINWFAVHTTNL
                     PNTYTKICSDNKGYAADNLERTMQKKYPNYIGAFAQGACGDVSARVRYNPKLSFQRGK
                     FEGVYSDDLKSSKFNGMLQFEKAREIIQDFTAPVKNDEVDAVLQYIDFSHIDIKPKFN
                     NGQEGGLTSPSVMGVSFLEGSKMDGPGMHPLIGNVSRGISNRLKSRELNQAKNLSDKD
                     AETITRRYHAQGNKHLALETGEKRLFGNADITKFFVPGFADPIIYNLKYFSRLGLMSE
                     HAWAPQILPVQIFKLGTVAIVAIPFEITTVASWRLRKTVEDALFPNGIEHVILCPYSN
                     AYNGYITTYEEYQVQDYEGGHCVFGQWSLNALQQVSHHLSLQLLNKPEDRDIPFLAEA
                     PISEQYLQRFAYEKSYYYKRIEKRTDRKENFILKKGKKYYERIDNSFK"
BASE COUNT         1034 a          635 c          667 g         1114 t
ORIGIN      
        1 aattatttaa atccattttt ttgataatac cattactgga ataagtcaga atatgatcat
       61 tcaacaaacc cggattggat tcattataaa cagtattgat tttgctgcct aagttttcgg
      121 gcttgctcca tttgttattt ttaaattctg agacatataa atccaaacca ccaacagaat
      181 taggtaatcc ggctacaaag tacatttttt ttgtagagcc gttgtcaagc aaagccggtt
      241 gacccacttt aaaagtaccc agcagttgag gatttaacgt atacgccacc cattttttgt
      301 ctttccatgt agcatgcagg atatcaaaca ttaatgtttc ttccaaagat tgtatgtgcg
      361 caggcacttg atagggaggt acattttcat tatacatgtt aaatgtaata ttttccagaa
      421 agccataatc attactcagc gtataagcag aaacggtatg agccaataaa atggacagtg
      481 tataaattaa gacatgcttt ttcattattt gaagtttaac tgttacaaaa gtgctgttta
      541 gatttataaa tatggatagg tagttttcca caataattga taattgtatt gcaatttaaa
      601 tgccatactc tgtatttttc actttattag cttttttcgt caaatgttat attttcataa
      661 aaaaaatttt tactttggca gtattgaata tgaacagatt atacaggaat ataaagtgta
      721 tcgtactgac atcagtattc tgtttagtat tcgttagcca agtggctgct ttaggcgaag
      781 ttaaaattgc aggagcccgg caatcgggca tgggatttag ctctattggg ttggtaagcg
      841 tttattctgc ttacaataat caggcggcag gtgcgttttt ggaacacccc ggagtgggtt
      901 tgtactatgc gcctgtattt ataggggagg gggtgagcaa tatttcggga gtggtagcag
      961 ttcctgttaa aaaagcggga acttttgggt tttctgtcaa ttatttcgga tatagccttt
     1021 ataatgaaaa aaaattcggt atttcgtatg caatcaaatt ggcgaaatat ttgtcgatag
     1081 gcatgcaatt ggattatctg aatgcaaaaa tttcaggtta tggttcaaaa aattacgcca
     1141 cctttgagct tggatttttc agtaaaccca tagatgaact ggcgattgca ttgcatgttt
     1201 ataatccgtt gaaattatat gtagacaaaa atacaggaga aaagattccc agtgtattgc
     1261 ggttaggaat tacctatgag gccagaaaga aatttttcat ctccacacaa attgataaag
     1321 attttacaca aaaaggggtt gtcttcagag caggagtgga atatacttta aaagacttaa
     1381 tcagtttcag agcaggagta gcttcgtcgc ctgtaacggg cagttttggt ataggtgttt
     1441 ctattcggca aggtgttacg atagatgctg cattttcata catgagtaca ttaggttttc
     1501 agcctcattt cgggtttatt tatgaactga agaaaaaaac caaagttggc ggcacaacat
     1561 ccaaaaagaa atcgaagaag aagtaagtcc acttaaactg aaacttattt gaaactgttg
     1621 tcaatccgtt cgtaatattt ttttcccttt tttaaaatga aattctcttt cctgtctgtt
     1681 cgcttttcta tgcgtttgta ataatagctt ttctcataag caaagcgttg cagatactgt
     1741 tcggaaattg gtgcttctgc caaaaatgga atatccctgt cttcgggctt attcagcagt
     1801 tgaagactta aatgatggct tacttgttgc agggcattca aactccattg cccgaaaaca
     1861 caatgcccgc cttcataatc ttgcacctga tattcttcat acgtagtaat atacccgttg
     1921 taggcattgg aataaggaca caagatgaca tgctctatac catttggaaa caaggcatct
     1981 tctaccgttt ttctcaaacg ccagctggct acggtggtta tctcaaaagg tatggccacg
     2041 atggctactg tacctaattt aaatatttgt acgggcaaaa tctgcggagc ccaggcatgt
     2101 tcactcatta agcccaaacg agagaaatat tttaagttgt aaatgatagg gtcggcaaag
     2161 cccggaacaa aaaacttagt aatatcagca ttgccgaaca gccttttttc tccggtttcc
     2221 aaggccagat gtttgttgcc ttgtgcatga taacgacgcg tgattgtttc cgcatcttta
     2281 tcactgagat ttttagcctg attgagttct ctgcttttta gccgatttga aatgccacga
     2341 ctcacatttc ctatcagcgg atgcataccg ggaccgtcca ttttagaacc ttccaaaaaa
     2401 gaaaccccca ttacagacgg tgaagtaagt ccgccttctt gtccgttgtt aaatttgggt
     2461 ttaatatcta tatggctaaa gtcaatatat tgcaaaacgg catctacttc gtcattcttt
     2521 acaggtgcag taaaatcctg aataatttct ctggcttttt caaactgcag catgccgttg
     2581 aatttggagc ttttcaaatc atccgaatac accccttcaa atttgccccg ctgaaaagat
     2641 aatttaggat tatagcgcac tcttgccgaa acatcaccac aagcaccctg cgcaaaagcc
     2701 ccaatataat tgggatattt tttctgcatc gtgcgctcca aattgtctgc agcatatcct
     2761 ttattgtcag agcatatttt agtataagta ttgggcaagt tggtggtatg taccgcaaac
     2821 caattaatag agccgagcaa tcttccatcc tcggctacaa attgcaagag cgtcatttct
     2881 ctgtcaacgg ccaaatggcg ttgagaaaag gaatattttt taacttcagg attttgattg
     2941 taagaattta ccgtgcggtt gaaagaaacg ggagtatcat cttcaaaaga agatttgccg
     3001 atagagatgt ctgcttttgt cttgttttta ttggcttcta tcaaagcttc agtgacgccg
     3061 tcaacaatcg ttttggtaat atgtgctata taaccgggaa cagaaatatt ataaatagca
     3121 tgcgcagaat atcctgccgg actgcaatgg gtatgctgtg cgcaaagcat taaattttgg
     3181 tcgtttattt ttaattccgg agcatgtttt tgtaaatttt tcactacttc ttgttttaaa
     3241 tgcggggtaa taaaacacaa ttcacaattt acaatggcaa ctttatgctg attgtcaaga
     3301 tcttctatga tataagcacg agcatacaac ggagtttcta catccagagc aacatgcgta
     3361 taaagaccat aacccaacat tcccgaacct ctttcaaaag cggtaatatc agctttacct
     3421 aaaccgatta aaaacatttt tgtaattttg
//
LOCUS       DAOQII010000263         3445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_17555_length_3445_cov_4.674631, whole genome shotgun sequence.
ACCESSION   DAOQII010000263 DAOQII010000000
VERSION     DAOQII010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17555_length_3445_cov_4.674631"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLF48_10610"
     CDS             complement(<1..440)
                     /locus_tag="PLF48_10610"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF49823.1"
                     /translation="MELTLTLQTYPMAEIITLDNDKLTQCNTKYPVLLVSGLGFHDNH
                     RLMSYWGNIPNYLKEHGCTVFTANQEAFLSIPDNAIKLKFRILDILEKTGAQKINLIG
                     HSKGGLESRYVASRMGMEDAISSITTLGTPHHGTHLADIILGKLP"
     gene            639..1472
                     /locus_tag="PLF48_10615"
     CDS             639..1472
                     /locus_tag="PLF48_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNF49824.1"
                     /translation="MKIGIQGFKASFHEIAALKFFGNDIETVDCATFGKLFATLQSGE
                     ADYAICAIENSLAGAILPNYALLRNHDFEIFGEVYLRIEMNLMALPNQRIEDLEEVHS
                     HPIALLQCRNFFEPYPNIQLIESFDTALSAEEIKKHHIRHRGAIASKRAAEIYDLEIL
                     AEDIHDNKRNFTRFLAIKKRNQPALLNHAIINKASLSFRAHHSPGSLAKVLTIIGDKN
                     INLTKIQSLPVIGEEWQYYMYIDLEFTDESDFRAIINDIQPYTTELKILGAYAAGEKL
                     M"
     gene            complement(1715..3070)
                     /locus_tag="PLF48_10620"
     CDS             complement(1715..3070)
                     /locus_tag="PLF48_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49825.1"
                     /translation="MLTSKDRIPFITGIGIALFVNFFSWQNPFFWDTLLTSTITQHYY
                     ENGFHNLILPTQIDAGHPPFFYVCITLFYKIIGKNLFTAHLAMLPIILSGVIAFILLL
                     RYFGFTLLQQVLAILTFFAIPHVLTQYSLVSYDATLLSLYLWALWAILKAKNGLLTVL
                     LILILAISLRGMVLWGALVVTTWYIYKIELRKFLFPFVISIFVITCWYIYHHWQCGWW
                     FSTPAAGWANQRGWADFPQLLKNIFSIARCFFDLGIVVLSLLFVISLFTKKYNKQLVG
                     LVLIPALLLSLSLLPFSNPVNHRYYLIVYVLLLLNVLHFMNQFNFRWSLMLILFLVFG
                     NKMIYPVPISNAWDCTMLHRNYFTTKKAIFRQMDEMGISRTQTGTTFPLDAGMHQSDM
                     TADTVRMINVNGSSLDQVKYVLFSNVCNDFSNDQIEEIKTWNKLYRVSDGQVYIELYE
                     K"
     gene            3118..>3445
                     /locus_tag="PLF48_10625"
     CDS             3118..>3445
                     /locus_tag="PLF48_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNF49826.1"
                     /translation="MKLSLAISPCPNDTFIFDALIHHKIDTEGIEFDVHFADVEALNT
                     AALEQQYDITKLSYFAYAFVSDYYILSHSGSALGNNCGPLLIAKSILNDDEIKQAKIG
                     IPGNYTT"
BASE COUNT         1189 a          614 c          595 g         1047 t
ORIGIN      
        1 ggtaatttcc ccaaaataat atcggctaaa tgtgtaccat gatgcggtgt gcctaaagtg
       61 gtgatgctgg aaatagcatc ttccataccc atcctcgaag ctacatatct ggattcgaga
      121 ccacccttac tgtgtccgat tagattgatt ttctgagctc cggttttctc caaaatatcc
      181 aaaattctga acttcagttt tatggcatta tccggtatgg aaagaaaggc ttcctgatta
      241 gcagtaaaca ctgtacatcc atgttctttt aaataattgg gaatgttacc ccaatagctc
      301 atcaaacggt gattatcatg aaaaccaagt ccacttacca ataaaacagg atatttggta
      361 ttgcattgag tgagtttgtc gttatcgagc gtaattattt ctgccatcgg ataagtttgc
      421 aatgttaaag taagttccat aaaaaatcaa tgttttgtgt cagatttttt gtggttaact
      481 atcaaataac atagtctttg ttgtaattgg cttgaaaact tgcttaattt gtagcatccg
      541 tttgttataa ctgaatggtt agtgaagaat taaatccctg taaaaatgta agctaaaaaa
      601 taaacactta ataaaacgga aattaaaatg tatataagat gaaaattggc attcaaggat
      661 ttaaagcttc gtttcacgag atagcagcac taaaattttt tggaaatgat attgaaacag
      721 ttgactgcgc aacgtttgga aaactgtttg ccacactgca atccggcgag gctgattatg
      781 caatttgtgc aatagaaaat tctttagccg gtgcaatctt gccgaattat gccttgctgc
      841 gaaatcatga ttttgaaatt tttggtgaag tttatttgcg tattgaaatg aatttaatgg
      901 cactgcccaa tcaaaggata gaagatctgg aagaagtaca ctcccaccct attgccttat
      961 tgcagtgcag aaactttttt gagccctacc caaatatcca gctaatcgaa tcttttgata
     1021 cggccttgag tgctgaagaa ataaaaaaac atcatatccg gcacagaggt gccatagcca
     1081 gcaaacgtgc cgctgagata tatgatttgg aaattttggc agaagatatt cacgataaca
     1141 aacggaattt cactaggttt ttagccatca aaaagagaaa ccaacctgct ctccttaatc
     1201 atgcaattat taataaagct tccctttctt tcagagcaca ccattcaccc ggcagtctgg
     1261 ctaaagtgtt gactataatc ggtgataaaa atatcaacct gacaaagatt caatctttac
     1321 ctgttatcgg cgaagaatgg caatattata tgtatataga tctggaattt actgacgaat
     1381 ccgatttcag agcaataata aatgatattc aaccctatac taccgaatta aaaatcttgg
     1441 gtgcttatgc tgccggagaa aaactgatgt aaactattct gcggtttaaa ctacatagaa
     1501 acatagcatt tttaacataa gaagttattt ggtcattcag tatagataat caagcaaaaa
     1561 catagtatcg ctttaggcga tacctatatt tttctattca taccagatat tgtttggtaa
     1621 gtattgttaa acctaaaaaa aatctatgcc tctatgtggt gaaaattata aatcaaccca
     1681 caaaagaata tgaattaatt cagttttatc ttctttattt ttcataaagt tcaatataca
     1741 cttgcccgtc tgacacacgg tataatttat tccatgtctt tatttcttca atttgatcgt
     1801 ttgaaaaatc attgcagaca ttagaaaaca gtacgtattt tacttgatcc aaactacttc
     1861 catttacatt gatcatgcgt actgtatccg ctgtcatatc cgattgatgc ataccggcat
     1921 ccaatggaaa tgtagttccg gtttgagttc gggatatacc catttcatcc atttgccgaa
     1981 agatggcctt tttagttgta aaataatttc tgtgtaacat cgtacaatcc catgcattgg
     2041 aaatgggtac cggatatatc attttattgc caaagactaa gaaaagaatc agcatcaaac
     2101 tccacctgaa attaaattga ttcataaaat gcaatacatt aagcaaaagc aaaacataga
     2161 ctatcagata ataacggtgg ttgacaggat ttgaaaaggg taacaaactt agtgacaata
     2221 ataaggcagg aatcagtact aatccaacta actgtttatt atactttttt gtaaatagcg
     2281 agatgacaaa caaaagactt aagacgacta tacccaaatc aaaaaaacaa cgtgcaatag
     2341 aaaatatatt tttcagcaat tgtgggaaat cagcccagcc tctttgattt gcccatcctg
     2401 cagccggagt agagaaccac catccgcatt gccaatgatg ataaatatac caacaagtaa
     2461 ttacaaagat ggaaataaca aaaggaaata aaaacttgcg taattcaatc ttatatatat
     2521 accaagttgt aactacaagc gcaccccata ataccatacc tcttaaactt attgcaagta
     2581 ttaaaatcaa aagaacagtt aagagtccat ttttagcttt aagtattgcc cataatgccc
     2641 ataaatataa acttaatagt gtggcatcat aactgactaa gctatattgc gtcaatacat
     2701 gtggaatggc aaaaaaagtc aggatggcta aaacttgctg cagcagcgta aacccaaaat
     2761 atctgagtaa taaaataaaa gctattactc cagacagaat aatgggcaac atggctaaat
     2821 gtgcagtaaa taaatttttt ccaataattt tgtaaaataa tgtaatacag acataaaaga
     2881 atggcggatg acctgcatct atctgtgtgg gcaaaatcag attatggaag ccattttcgt
     2941 aataatgctg agtgattgta gatgtgagta gtgtatccca gaaaaacgga ttttgccatg
     3001 aaaaaaagtt gacaaataaa gcaatgccaa tgcctgtaat aaaaggtatt ctgtctttag
     3061 atgtcagcat agcgcaaaga taacataagt accctgatta aatcgtaact ttgaatcatg
     3121 aaattgtcgc ttgctatttc gccttgtccg aatgatacct ttatcttcga tgcactgatt
     3181 catcacaaaa ttgacacgga aggcatagag tttgatgttc attttgcaga tgtggaagcc
     3241 cttaatactg cggctttaga gcagcaatat gatatcacca agttgagtta ttttgcttat
     3301 gcttttgttt ctgattatta tatcttatct cattccggaa gtgcgttagg taataattgc
     3361 ggccccttac tcatcgccaa atccatactg aatgatgatg aaataaaaca agctaaaatt
     3421 ggtattcccg gaaattacac cacag
//
LOCUS       DAOQII010000264         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_17650_length_3434_cov_3.925422, whole genome shotgun sequence.
ACCESSION   DAOQII010000264 DAOQII010000000
VERSION     DAOQII010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17650_length_3434_cov_3.925422"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3434)
                     /locus_tag="PLF48_10630"
     CDS             complement(<1..>3434)
                     /locus_tag="PLF48_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNF49827.1"
                     /translation="DIGLIVKQDKSAVHVFANSIKTTEPIKNAEISIISTSNQELGKV
                     KTDGDGYAHFVIPASYKNIGFKVGMVTAVHDTDFNYIILDGKEVDNSRYDVGGKYLNA
                     SNYDAYIYAERDIYRPGETMHLNTIVRTNTWELPGEMPVKIKIFAPNGKEFYNAKNVL
                     NEQGAVSDDIALSSSAMTGTYVYQIYSGNDILLASKNMLVEEFMPDRIKVDLKTDKEE
                     YRPNEAVNISINAMNYFGPPAANRNYEVNFSLEKASFAAKEFKQYTFDVSKAAEYLSV
                     NANGITDANGNASAAFNIPAEYASTGLLSGRAFVTVFDETGRPVNRLKTFNVFTQDTF
                     YGIKTGNDYFSTKSLIKIPVVAVDKKGVALTSAKANVKMIKRTWETVLQKNNGVLSYV
                     SQQKEEVVENKNILIRGANSYFAFSANESGEYEMRLSNPGSESYVSFNFWAYGWGDTY
                     ANSFEVNNEGKIDITLDKEKYLCGEKAKVLLKMPFDGKVLISIERDSLIEHFYIDSKN
                     KAAEFELKLKEVHVPNVYVSAVLIKPNVNDGMPLTIAYGFQPVLVENPDNKIPIDIQC
                     ATESRSKTKQVIKIKSKPNTEMTIAVVDEGILALKNQKSPSPYDYFYSKRALEVSLFS
                     IYPYLFPELTALGGGFDDIGKRVNPMTNKRIKLVSLWSGIIKTNALGNAEFPIYIPQF
                     SGSLRVMAVAYNGKRFGNADKNITVADPIVISTGIPRFVSPGDELTVPVTVSNTTKNN
                     AQAIVSVQSFGVLQNKVNGEMTTDITAKSEKQLDFSVITKEMTGEGKVVVTVKSMNET
                     FSDTTYINSRPNTSYRKYSGSSTVDGGKSATVSFAGNMPMMFHQNKLVVGNSPLIQFG
                     KNLQYLVQYPYGCIEQTISSAFPQLYYTDLAKNLNEVSGQKNNAVSNVQFAIDKVQSM
                     QLSSGAMAYWPGGNYDTWWGTVYAGHFLLEAQKAGYNVNPKVLNKLFEYLQNRMKIKE
                     VEWMYYADGRKREVIKEEVPYSLYVLAMADEVKGSTLNYYKSNKELLTQSGQYLLACA
                     YAMQGDKKKFYELLPKGYVNENADPKFSGNFYSDFRDMALVLNTLLEIDPQNTQINYM
                     AKQVSQKLLQSTYLNTQENVFGILAMGKIARNANKSTVTASIMSNGKEIAKFDGKNV"
BASE COUNT          968 a          710 c          580 g         1176 t
ORIGIN      
        1 acgtttttac catcaaactt ggctatttct tttccgttag acataatact tgccgttacg
       61 gtagatttat tggcatttct ggcaatttta cccatagcca aaataccgaa tacattttcc
      121 tgtgtattga gataagtact ttgcaacagt ttttgagaaa cttgttttgc catgtaatta
      181 atttgcgtat tctgaggatc tatttctaaa agtgtattca ataccaatgc catatctctg
      241 aaatcggaat aaaagttgcc gctaaatttc ggatcagcat tttcatttac atatccttta
      301 ggaagcaatt cataaaattt ctttttatct ccttgcatgg cataagcgca agccagcaaa
      361 tactgaccac tttgcgtcag cagctcttta tttgatttgt aataattcag ggtagagcct
      421 tttacttcat cagccatggc cagcacatac aaggaataag gcacttcttc tttaattact
      481 tcccgttttc tgccatctgc ataatacatc cattccactt cttttatttt cattctgttt
      541 tgcaaatact caaacagttt attcaatact ttagggttta cattatatcc tgccttttga
      601 gcttccagta aaaaatgtcc ggcataaact gtaccccacc aagtgtcgta attgcccccc
      661 ggccagtacg ccatagcacc actggataat tgcatagact gtaccttatc aatggcaaac
      721 tgcacattgc tcactgcatt atttttttgc ccggatactt cgttcaaatt ttttgccaaa
      781 tcggtataat acaattgtgg aaatgcagat gatatagttt gctcaataca gccataagga
      841 tattgcacta agtactgtaa atttttacca aactgtatca atggcgaatt gcccactact
      901 aatttattct gatgaaacat catcggcata tttccggcaa aacttacagt tgccgattta
      961 ccgccgtcta cggtactgct gccggaatat tttctataag aagtatttgg acgagaattg
     1021 atataggtgg tatcgctgaa tgtttcattc atcgatttta cagtaaccac tactttgcct
     1081 tcgcccgtca tctctttagt gattacggaa aaatcgagtt gtttttcact tttagccgta
     1141 atatcagttg tcatttcacc atttacttta ttttgcaata caccaaagct ttgaacactg
     1201 acaatggctt gtgcattatt tttagtggta ttacttactg taacaggaac ggtaagctca
     1261 tcgccgggcg atacaaatcg cggaatgccg gtgctgataa cgataggatc tgcgactgta
     1321 atatttttat ctgcattgcc aaaacgcttt ccattgtatg caacagccat cacacgtaaa
     1381 ctccctgaaa actgcggaat ataaatggga aattcggcat tgcccagtgc atttgttttg
     1441 ataataccag accaaagtga taccagtttt atgcgtttat ttgtcatcgg atttactcgt
     1501 tttccaatat catcgaagcc accgcccaag gctgttagtt caggaaataa atagggataa
     1561 atggaaaaca aactgacctc taaagcccgt ttagaataga aataatcata agggcttggt
     1621 gatttttgat ttttgagtgc caatattcct tcatctacta cagcaatcgt catttctgta
     1681 ttaggtttcg attttatttt aattacttgt ttggttttag agcgtgattc tgttgcacat
     1741 tgaatgtcaa tcggaatttt attatcggga ttttctacta ataccggctg aaaaccataa
     1801 gcaatggtga gtggcatgcc atcgtttaca tttggtttta tcagcactgc cgaaacatag
     1861 acattaggta catgaacttc cttaagtttt aactcaaact ctgctgcttt gtttttactg
     1921 tcgatataaa aatgctcaat cagactatct cgctctatgg aaattaaaac tttgccatca
     1981 aacggcattt tcagcaatac ttttgccttc tcgccgcata agtatttttc cttatcgagt
     2041 gtgatgtcta tttttccttc attattaacc tcaaaagagt tggcatatgt atcgccccag
     2101 ccataggccc aaaaattgaa tgacacatac gactcgcttc cgggattgga caagcgcatt
     2161 tcgtattctc cgctttcatt cgcagaaaat gcaaaataag aatttgcccc tcttatcaga
     2221 atatttttat tttctacaac ttcttctttt tgctgtgaaa cataactgag tacaccgtta
     2281 tttttttgca agacagtttc ccaagtccgt ttaatcattt ttacatttgc ttttgcagaa
     2341 gtcagtgcaa cgcctttttt atcaacagct acgaccggaa tttttatcag acttttcgta
     2401 ctgaaatagt cattgcctgt tttaatacca taaaaagtat cctgtgtaaa aacattaaat
     2461 gttttcaggc ggtttaccgg tcttcccgtt tcatcaaaga cggtaacaaa agctctgcca
     2521 ctgagcaatc ctgttgatgc atattcggca ggaatattaa atgcagcaga agcattacca
     2581 ttggcatctg taatgccatt agcgttcaca ctcagatatt ctgcagcttt actgacatca
     2641 aaagtatatt gtttaaattc tttagcagca aaggatgctt tctctaatga aaaattgact
     2701 tcatagtttc tgttggctgc cggaggccca aaataattca tggcatttat actgatattg
     2761 acagcttcat tgggacggta ttcttcttta tctgttttca aatccacttt aatcctgtca
     2821 ggcataaatt cttctaccag catatttttt gaagccagta aaatatcgtt gcctgaatag
     2881 atttgataaa cataagtacc cgtcattgca gaacttgaca atgcgatatc atcagacact
     2941 gcaccttgct cattcaatac atttttggca ttataaaact ccttaccgtt aggagcgaaa
     3001 attttgattt tcacgggcat ctctcccggc aactcccaag tattagtacg tactattgtg
     3061 ttgaggtgca tggtttcacc cggccgatag atatctcttt cggcataaat atatgcatca
     3121 taattcgagg cattcaagta ttttccacca acatcataac gagaattgtc gacttctttt
     3181 ccgtccagta tgatgtagtt aaaatcggtg tcgtgcacag cagttaccat tcccacctta
     3241 aagccgatat ttttataaga tgccggtatg acaaaatgag catagccgtc gccatctgtt
     3301 tttacttttc ccaattcttg attggatgtt gatataattg agatctctgc gttcttgata
     3361 ggttcggtag tttttattga attagcaaaa acgtgaacag ctgatttatc ttgttttact
     3421 attaagccaa tatc
//
LOCUS       DAOQII010000265         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_17784_length_3418_cov_6.905442, whole genome shotgun sequence.
ACCESSION   DAOQII010000265 DAOQII010000000
VERSION     DAOQII010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17784_length_3418_cov_6.905442"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLF48_10635"
     CDS             <1..485
                     /locus_tag="PLF48_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49828.1"
                     /translation="TPDFKKSVKSLIDKAKPGGEIAVDFYPIKGWYTKLSSKYIFRPW
                     TKKMSHEKLLNKIDRNADRLIKTYKVIDKMGLAMIGHRFLPMCDINRTLPANLSKEEL
                     REWVVLDTFDMFSPAYDNPQKISTVKKWMEDCGVKVTQAGFLTYANNLKVAVVRGIKK
                     "
     gene            660..1604
                     /locus_tag="PLF48_10640"
     CDS             660..1604
                     /locus_tag="PLF48_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49829.1"
                     /translation="MKRKLFRADIIFFSLFVISAVFTLLQLSGRYNPLTRFDTSPPKN
                     ERQEEYEASLSRINSVRKLEVYSDSLYNATYSQINKEADYEKKYSNLLAAIVRKRFYH
                     GYSEYGFSDNYLLTLFAKITNAAYNSVIAPDDVLKYAYASCSQQSVVMMELLNRKNIS
                     TRKVGFLGKKFGGHFSFEAYYNGQWHFFDTNMEPDLAVLNAYDRPGISFLTQHTELLT
                     TAYRRYNADKILDIFPKYAYGAVNVFPAPRGLLFQKITGILSYSLWFFFFILFIITRR
                     LYKRQTVSNYVWNRRIHFPPFFAGAPSVSHPGHKASGA"
     gene            1516..3366
                     /gene="asnB"
                     /locus_tag="PLF48_10645"
     CDS             1516..3366
                     /gene="asnB"
                     /locus_tag="PLF48_10645"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNF49830.1"
                     /translation="MCGIAGFISHRFSQEHLQCLTQAIKHRGPDAEGFYYDNNSGIGL
                     GHRRLSIIDLSEAANQPFYSHDGRYVMVYNGEVYNYKEVAAKYGIQQRTTSDSEVIIE
                     AFALKGTNSFSDLNGMFAFAIWDKHLKKLTLARDHIGIKPLYISVSEQELLFASEIKA
                     ITAIKKDLTINKKVVPSFLHLGYIPHPFSIYNEVIKLDAGSFIEISVSDAGTLSYHSQ
                     SFWQVSDKIAKTTLSDEQSAKNILDSLLTRSIAGQLVSDVPVGTFLSGGTDSSLVTAL
                     ACKVSDKKVNSFSIAVTDGKINEAPYAAAVAKHLKTDHYELPITQKEILEMVPDFMSV
                     YDEPFTDSSAFPTMLVSKLARQHVTVTLSGDGGDELFGGYGRYLWVEKLQSPLYKTFS
                     PVVYAATRFLGSGYKRAGNMFEKYPAGNFKSHLFSQTESFFSENELKNLLVESDFNFS
                     SLNSSFNGRKLNAFEEAAFWDIENYLKDDLLVKVDRASMRYSLETRVPLLDYKVVEFA
                     LNLSPELKINKNGTAKYLLKQILYDYVPKELLERPKWGFSIPLVKWLKTDLKWMIDKY
                     CSKEMIEQAGIVRYDKVKQLIDLYLGNKNDYLYNRIWSLIVLHWFFYDRR"
BASE COUNT         1134 a          542 c          682 g         1060 t
ORIGIN      
        1 atacacctga ttttaaaaaa tcggtaaaaa gtctgataga taaggcaaaa cccggtggag
       61 aaattgcagt tgatttttat ccaatcaaag ggtggtacac taagctcagt tcaaagtata
      121 ttttcagacc ctggacaaaa aaaatgtcac atgaaaaact attgaataaa atagaccgca
      181 atgctgacag gctgattaaa acttataaag taattgataa aatgggactg gcaatgatag
      241 gtcatcggtt tttaccaatg tgcgatatca acagaacatt acctgctaat ttatcaaaag
      301 aagaattaag agagtgggta gtgctcgaca cttttgatat gttttctccg gcttatgaca
      361 atccacaaaa aataagcact gtaaaaaaat ggatggagga ttgtggggta aaagttacgc
      421 aagcaggttt tttaacttat gccaacaatt taaaagtggc tgtggtgaga gggataaaaa
      481 agtaatgaat aggatacatg ttataatccg taaaaatact aacgagttcg gagtttcact
      541 aaaccaatac agagtataac cgctctataa atgatttttt gtgtttatgt atttgttcat
      601 gaaaacagat agctccaatt tagtagcagc aaatccaata tcagaacaaa gttgttgcta
      661 tgaaaagaaa actgtttcgg gcggatataa ttttttttag tctttttgtt atttctgctg
      721 tatttacatt gctccaatta tcaggacgtt acaatccttt aacaaggttt gatacatctc
      781 caccaaaaaa tgagagacaa gaagaatatg aagcatcact ttcgagaatc aattctgtac
      841 gcaaactgga agtatatagc gacagtcttt ataatgcaac ttattcacag ataaataaag
      901 aagcagatta cgaaaaaaag tattcaaatc tgctcgcagc tattgtcaga aaaagatttt
      961 atcatggtta ctccgaatat ggcttcagtg ataattatct gttgacttta tttgcaaaaa
     1021 ttaccaatgc agcatataat tctgttatag ctcccgatga tgtgctgaaa tatgcttatg
     1081 cttcctgcag tcagcagtct gtagtaatga tggaattgtt aaatagaaaa aatatttcaa
     1141 ccaggaaggt cgggtttttg ggaaagaagt ttggaggtca tttttctttt gaagcatatt
     1201 acaacggcca atggcatttt tttgatacca atatggagcc tgatttagca gtattaaacg
     1261 cttacgacag gccgggtatt tcttttttga cacagcatac tgaattgctt accacagcat
     1321 acaggcggta caatgcagat aaaattctgg atatttttcc gaaatatgct tatggagcag
     1381 ttaatgtttt cccggctccc cgcgggttac tctttcagaa aataaccgga atactttctt
     1441 attcactttg gtttttcttt tttattcttt ttataattac ccgtcgctta tacaagcggc
     1501 aaacagtttc taactatgtg tggaatcgca ggattcattt cccaccgttt ttcgcaggag
     1561 caccttcagt gtctcaccca ggccataaag catcgggggc ctgatgccga aggattctat
     1621 tacgacaata attccggcat tggacttggc catcgcaggt tgagtattat cgacttgtct
     1681 gaagctgcca atcagccgtt ttattctcat gatggaagat atgtgatggt ctataatggg
     1741 gaagtatata actataaaga ggtagctgcg aaatatggta ttcagcaaag aaccacgtct
     1801 gactccgaag ttatcataga ggcattcgct ttaaaaggaa ctaattcgtt cagtgacttg
     1861 aacggaatgt ttgcttttgc aatatgggat aagcatttaa aaaaattaac cttagcaaga
     1921 gatcatatag ggataaaacc actgtatata tctgttagtg aacaagagct tctttttgca
     1981 tcagagataa aagcaattac ggcaatcaaa aaagatttga caataaataa aaaagtggtt
     2041 ccttcatttt tacatttagg ttatatacca cacccttttt caatttataa tgaagtaata
     2101 aaacttgacg ccggctcatt tattgaaata tcagtaagtg atgccggtac tctttcatat
     2161 cacagccaat cattttggca ggtaagcgat aaaatagcga agactacctt atcggatgaa
     2221 caatctgcta aaaacatttt agattcttta cttactcgtt ccattgccgg tcaattggta
     2281 agtgacgtac ctgtcggaac atttctgagt ggtggtacag atagcagctt ggtaactgct
     2341 cttgcttgca aggtgtcaga caaaaaagta aactcattta gtattgcagt taccgatgga
     2401 aaaattaatg aagcacctta tgcagcagcc gtagcgaagc atttaaaaac agatcattac
     2461 gaattaccga taacacagaa agaaattttg gaaatggttc ctgattttat gagtgtttat
     2521 gatgaaccat ttaccgactc ttctgcattt ccgaccatgt tggtaagtaa actggcccgg
     2581 cagcatgtta ctgttaccct aagcggtgat ggcggtgatg aactgtttgg cgggtatggc
     2641 cgttatttgt gggtagaaaa attacaaagt ccattatata aaactttttc acctgttgtt
     2701 tatgctgcga cgagattttt gggaagcgga tataaacgag caggtaatat gtttgaaaaa
     2761 tatcctgccg gaaattttaa aagccattta ttcagtcaga cagaatcttt tttttctgaa
     2821 aatgagttaa aaaatttgtt ggttgaatcc gattttaatt tttcctcatt aaacagttca
     2881 tttaatggaa gaaaattaaa tgcatttgaa gaagctgcat tttgggatat tgaaaattat
     2941 ctgaaagatg atcttctggt aaaagtggac agggcaagta tgagatatag tctagaaaca
     3001 agagtaccct tattagatta taaggtggtg gaatttgcat tgaatctttc accggagtta
     3061 aaaataaaca agaacggaac tgcaaagtat ttgctgaaac aaatattata tgattacgtt
     3121 cccaaagagt tgctggaaag accgaaatgg ggttttagta ttccgctggt aaagtggttg
     3181 aagacggatt taaaatggat gattgataaa tattgttcaa aggagatgat agaacaagcg
     3241 ggaatcgtaa gatatgataa agtaaaacaa cttattgatc tttatcttgg aaataaaaat
     3301 gattatttat acaaccgaat ttggtcgttg attgtattac attggttttt ttatgatcgc
     3361 aggtaaaaaa atcctttaca tatcttatga tggtatgacg gacccgctgg ggcaaagt
//
LOCUS       DAOQII010000266         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_17788_length_3418_cov_4.463574, whole genome shotgun sequence.
ACCESSION   DAOQII010000266 DAOQII010000000
VERSION     DAOQII010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17788_length_3418_cov_4.463574"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..2036
                     /locus_tag="PLF48_10650"
     CDS             273..2036
                     /locus_tag="PLF48_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF49831.1"
                     /translation="MASKILIKNGLLFTGDKDQKAEICHMLIEGNKIKQISKSEILTD
                     PETEVIDATDCWITPGFIDTHTHYDAELVASPRLKESARHGVTTVVVGSCSVSAIYNN
                     PEDTSDTFTRVEAIPREVMLPLMEKTKTWHTPKEWKTFINDSALGVNVASFIGHSDVR
                     SKVMGLKRSVTENETATFQEQEEMNKMLNDALDVGFLGLSTMDNPWDKMDGNRYWSRK
                     TPSFYGSWKERKLMIDTLRKRNAILQGAPNLVTRVSALVYMMKSSGIFRKPLKTTMIA
                     MVDLIGDRFIMPLTMFGSNLTNRLLKGNFRMQAPPAPFTVYYDGVDSVMFEEFPTGEA
                     MRHLAKNLHERNTLIKDKTFREQFKKEIKNKYKPKVWHKDLSLSVVLDCPDKSLIGKN
                     FFQIAEERKQHPVDCFLNLIVDYDKKIRWTTTIGNDRPEKYKVIYNFPFNIISFSDAG
                     AHLTNMAFYNFPLQMIKQVQKSIDNKTPIMSMEKCIWRLSKEQADWLGLDACFLAEGK
                     IADINIINPKYLTEVKDDVQEDTIEEFDGFNRLVNRNDNLLQYVIVGGKIIYSNNQFT
                     PGYGKTFKSGRFLEKQDLVAG"
     gene            complement(2022..2360)
                     /gene="ytxJ"
                     /locus_tag="PLF48_10655"
     CDS             complement(2022..2360)
                     /gene="ytxJ"
                     /locus_tag="PLF48_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HNF49832.1"
                     /translation="MLHWNELLSGQQFKDLLNDETFFAVFKHSTRCSISDMAKNRLER
                     EWNLDLPIYYLDLLEHRDVSNLIAELSGIQHQSPQLLVFRFGKVIYHASHNSIDVQEL
                     TEIIDASPGN"
     gene            complement(2361..2501)
                     /locus_tag="PLF48_10660"
     CDS             complement(2361..2501)
                     /locus_tag="PLF48_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49833.1"
                     /translation="MRKLTKITLIIATCISITAMSCARRNATGCPAWGKTNQPKTTQK
                     SV"
     gene            2675..>3418
                     /locus_tag="PLF48_10665"
     CDS             2675..>3418
                     /locus_tag="PLF48_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNF49834.1"
                     /translation="MSNYHFIAIGGAVMHQLALHLKNEGHTVSGSDDAIFDPAKSNLI
                     SYGLMPDAEGWFPDRINQNIDAVILGMHAKADNPELIRAQELGLKIFSFPEFIYEQSK
                     SKTRVAVAGSHGKTSITSMIMHVLFTQHENFDYLVGAQLAGFDFMVKTSKDADSIILE
                     ADEYLTSPLDLRSKFLHYHPHIAVISGIAWDHINVFPTFEAYLETFRKFILSIQPGGC
                     LIYNAQDEVLVSLVEALQPSVQLIPYFPFE"
BASE COUNT         1091 a          594 c          677 g         1056 t
ORIGIN      
        1 ttttcatagt tggtattaaa attaaagcat tttttacttc tgcgatatta ttttttttga
       61 ccctaattag tttgtatcag cctgttctaa gttcaagtgt taaatgcatt tttttttcgg
      121 cataaaacaa cattttttga aaacagagaa gaggttatcc tatttgcgta tgatgacaat
      181 caacccattt aggcttacat tttttcttct taactttaat tattgttaca tgacatttaa
      241 taacgtaatt tcataataaa tttagttggc ttatggcttc aaaaatttta ataaagaacg
      301 gtctgctctt caccggtgat aaagatcaaa aggctgaaat ctgccacatg ctgatagaag
      361 gaaataagat aaaacaaatt tctaaaagtg agattttaac agatccggaa acggaagtta
      421 tcgatgctac agattgttgg attacacccg gatttatcga tacgcatacg cattatgacg
      481 cagagcttgt cgcatcaccc agattaaaag agtctgctcg ccacggtgta acaactgtgg
      541 ttgtgggcag ttgctcggta tcggcaattt acaataaccc cgaagatacc tcagatacat
      601 ttacccgagt ggaggctatt cccagagaag tgatgttgcc actcatggaa aaaacgaaaa
      661 cgtggcatac accgaaagaa tggaaaacat ttatcaatga ttcagctttg ggtgtaaatg
      721 tagcctcgtt tatcggacat tctgatgtgc gtagcaaagt aatgggattg aagcgttctg
      781 ttactgaaaa tgaaactgcc acctttcaag aacaagagga aatgaacaag atgctcaatg
      841 atgctttgga tgtaggattt ttgggtttgt caaccatgga taatccctgg gataaaatgg
      901 atggtaacag atactggtcg agaaaaacac catctttcta tggctcatgg aaagaacgca
      961 aactcatgat tgatacactg cgcaaacgca atgctatttt gcagggagca ccaaatttag
     1021 ttacccgggt aagtgctctt gtttatatga tgaaaagctc aggcatattt agaaaaccac
     1081 ttaaaacgac aatgattgcc atggtagatc taataggtga ccgcttcatt atgccactca
     1141 ccatgtttgg ctccaatctt accaacaggc tgttaaaagg aaatttcagg atgcaggctc
     1201 cacctgcacc gtttaccgta tattatgatg gagtggattc agtgatgttt gaagaatttc
     1261 caaccggcga agccatgcgt catttggcaa aaaatctgca tgagcgaaat acacttatta
     1321 aagacaaaac attcagagaa caatttaaaa aagaaattaa gaataaatac aaacctaagg
     1381 tttggcataa agatttgagt ttgtctgttg ttttggattg tccggataag tcactaatcg
     1441 gtaaaaactt ttttcaaatt gcagaagagc gtaaacaaca tccggtagat tgctttttaa
     1501 atttgatcgt tgactatgat aaaaaaatac gatggacaac cacaataggg aatgaccggc
     1561 ctgaaaaata taaggtgatt tacaatttcc catttaatat catcagcttt tccgatgcag
     1621 gcgcacattt aaccaatatg gcattttaca attttccatt acagatgata aaacaagttc
     1681 aaaaatctat cgataataag acaccaatta tgtcaatgga aaaatgtatc tggcgtttat
     1741 ccaaagaaca agccgactgg ctcggtttgg atgcctgttt tcttgcagaa ggaaaaattg
     1801 cggatatcaa tatcatcaac ccgaaatatt tgactgaggt taaggatgat gtacaagaag
     1861 atacgattga agaatttgac ggtttcaata gattagtgaa cagaaatgat aatctgttgc
     1921 aatatgtaat agtaggcgga aaaataatct atagcaataa ccaatttaca ccgggttatg
     1981 gcaaaacatt taaatccgga agatttttag aaaaacaaga tttagttgcc gggtgaggca
     2041 tcaataatct ctgttaattc ttgtacatca atactattat gggaagcgtg atagattact
     2101 tttccaaatc tgaagacaag cagttgaggt gactggtgct gaatgcctga taattctgca
     2161 attaaattgg aaacatcccg gtgttcgagc aaatcgagat aataaatggg taggtcaaga
     2221 ttccattcac gttcaagcct gttttttgcc atatcggaaa tagaacagcg tgtactgtgc
     2281 ttaaatacgg caaaaaacgt ttcatcattt aataaatctt tgaattgttg ccccgagaga
     2341 agttcgttcc aatgtaacat ttatacgctt ttttgtgtgg ttttaggctg atttgtttta
     2401 ccccaagccg gacaacctgt ggcatttcgg cgggcgcatg acatggctgt gattgaaatg
     2461 catgttgcga taattaatgt gattttagtt aactttctca tttgttgttg tttatacgaa
     2521 tacgcaaaaa taagacaatt attgtacgcg tccactttta taagtttttg ttatgatttg
     2581 ataacatttt gtaaaaatac taatctgtgt taaaatgttg aattgtttat aaaaattaga
     2641 ctgttgctta gcagctggtt tttattttta ccccatgtca aattatcatt ttatcgccat
     2701 tggcggtgcc gttatgcatc agctggcact gcatttaaaa aacgaaggac ataccgtttc
     2761 aggttcagat gatgccatct ttgatcctgc aaaatccaat ctaattagct atggtctgat
     2821 gccggatgcc gaaggctggt ttccagatcg cataaatcaa aacattgatg ccgtaatcct
     2881 tggtatgcat gccaaggcag ataatccgga gctgataaga gcacaagagc tcggtttaaa
     2941 aatattttca tttcctgagt ttatttacga gcagagcaaa tctaaaacac gagttgccgt
     3001 agcaggtagc catggtaaaa catctatcac ttccatgatt atgcatgtgt tgtttacaca
     3061 acatgaaaac tttgactacc tcgtaggtgc gcagttggca ggatttgatt ttatggtaaa
     3121 aacgtctaaa gatgccgatt cgataatatt agaagccgat gaatacctga catctccttt
     3181 ggatttgcgg agtaagtttt tacattatca cccgcatata gccgtaattt caggtattgc
     3241 ctgggatcat atcaatgttt ttcccacgtt tgaagcatat ctcgaaacgt ttcgcaagtt
     3301 tatcctcagt atacaacccg gtggttgttt gatttataac gcacaggatg aagtactggt
     3361 aagcttagtt gaagcgttgc aaccaagtgt tcaattaatt ccctatttcc catttgaa
//
LOCUS       DAOQII010000267         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_18116_length_3379_cov_5.152828, whole genome shotgun sequence.
ACCESSION   DAOQII010000267 DAOQII010000000
VERSION     DAOQII010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18116_length_3379_cov_5.152828"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLF48_10670"
     CDS             <1..295
                     /locus_tag="PLF48_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49835.1"
                     /translation="DLSLPMMFYSRPKGEYTGSETDAVLLDFYLVNCDLAKDGYKVKA
                     EINGTEFMLTKWAGYFMEGLPKGKNVIKLTLLDAKGNRVDNQFNPVERTITLK"
     gene            514..1797
                     /gene="eno"
                     /locus_tag="PLF48_10675"
     CDS             514..1797
                     /gene="eno"
                     /locus_tag="PLF48_10675"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNF49836.1"
                     /translation="MSYIISVHARQILDSRGNPTVEADVITENGVIGRAAVPSGASTG
                     IHEAVELRDNGKTYMGKSVLKAVNNVNTKIAEALVGESVLLQRDIDDILLALDGTENK
                     KKLGANAMLAVSLAAAKAGAQEAGLPLYRYVGGTNAHVLPIPLMNILNGGAHADNKID
                     FQEFMIAPVGADTFSDALRMGVEVFHHLKKVLKSKGYSTNVGDEGGFAPEIQSNEEAI
                     EIVLKAIETAGYKPGDQIMIALDPASSEFYDTKKKKYIFKKSDNRMLSSEEMVDYWAN
                     WVKKYPIVSIEDGLAEDDWKGWKLMTDTLGKKCQLVGDDLFVTNAKRLAEGIKNGVAN
                     SILVKVNQIGTLSETIDAVDLAHRNRYTSVMSHRSGETEDSTIADLAVALNCGQIKTG
                     SASRSDRIAKYNQLLRIEEELGDEAYYPGRGFRFY"
     gene            1866..2882
                     /locus_tag="PLF48_10680"
     CDS             1866..2882
                     /locus_tag="PLF48_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49837.1"
                     /translation="MPVFKKAIFIILVNLMCLNLKAQNCYSFYKLRLSATDTSFAKIE
                     NAFNKAIKKNKNEACLYAERSLLYRKFLKFDLALNDVNKAIKLQPEKYLFYEDKVDLL
                     LEIDSMASVKDLINTMMIMDSSIPNAYVFKGKYFLHLDSLEKAFEAFNKAITIYESNH
                     ANCCLMCECYRARGRLNFAFHKFDDAISDFTKAMKMVRVSKRDKYYLLRANAYIAKDD
                     LINANKDIDTAIKLKPDSFTLAFFYALKNDTLKLNSIIPYLLDKKINTEKQEYIKLYN
                     ISCLYAITNQTDKAFEFLEKSLNAGYKNFNWIQIDYDMKNISKLPKFNELLQQYRKHT
                     KSDE"
     gene            complement(3110..>3379)
                     /locus_tag="PLF48_10685"
     CDS             complement(3110..>3379)
                     /locus_tag="PLF48_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49838.1"
                     /translation="EKEQVMLYIENEHEQNEKLIIYDVKNKNEIISTDFEKSKCIEHV
                     IGGLRNCVEIDTVTSDKIILKVETDEERIIKKIQPPKQQLSFRWA"
BASE COUNT         1169 a          543 c          641 g         1026 t
ORIGIN      
        1 ggatttaagt ctgccaatga tgttttacag ccggcctaaa ggagaatata ccggcagcga
       61 aactgatgct gtcttattgg atttttattt ggtgaactgt gatttggcaa aagacggata
      121 taaagtgaaa gcggaaatca atggtactga gtttatgctc accaaatggg caggctattt
      181 tatggaagga ttgccaaaag ggaaaaatgt aatcaaatta acactattag atgccaaagg
      241 gaatagagtt gacaatcaat tcaatccggt agaaagaacg attacgctga aatagtgaag
      301 aaatgttgaa agttaaaaga tgatttttta ctttttgcca tctattcaac tgtcaactga
      361 aaaaaaattg tttaaccttt tctcacattc tatttttttt tgctaacatc tttttgcatc
      421 aatacaagat gccttagttt tttttatctt atttttgcag tgtaataaaa atgacttgtt
      481 cagtttacaa gaaaaacgca aaataatatt tttatgagct acattatttc cgtacatgcc
      541 cgtcaaattc ttgattcccg tggcaatcct acagtagaag cagatgtaat aaccgaaaac
      601 ggtgtaatag gcagggcagc agtgccttcc ggagcctcta ccggcataca tgaagctgta
      661 gaattgcgcg ataacggcaa aacctatatg ggcaaaagcg tattaaaagc agtaaacaat
      721 gtaaatacta aaattgccga agccctggta ggagagagcg tattgctgca acgagatatt
      781 gatgatatac ttttagcact ggatggtacg gaaaataaaa agaaattagg tgccaatgcc
      841 atgctagccg tatcgcttgc cgcagcaaaa gcgggggcac aagaagcagg cttgccctta
      901 tatcgctatg taggaggaac aaatgcacat gtattgccaa ttccgttaat gaatatcctc
      961 aatggaggag cacatgccga taataaaata gattttcagg aatttatgat tgcacctgta
     1021 ggagcagata ctttttccga tgctttgcga atgggcgtgg aagtttttca tcatttgaaa
     1081 aaagtattga aaagcaaagg ctattcaacc aatgtgggag atgaaggcgg ttttgctccc
     1141 gaaattcagt cgaatgaaga agccattgaa attgtgttga aagccataga aacggcagga
     1201 tataaacccg gtgatcaaat catgattgcc ttggatccgg catcatcaga attttacgat
     1261 acaaaaaaga aaaaatatat cttcaaaaaa tcggataaca gaatgttgtc ttctgaagaa
     1321 atggtggatt attgggcaaa ttgggtaaaa aaatacccga tcgtgtctat tgaggacggc
     1381 ttagccgaag atgactggaa aggctggaag ctgatgaccg atacactcgg taaaaaatgc
     1441 cagttggtag gcgatgattt gtttgtaact aatgcaaaac gattagcaga aggaattaaa
     1501 aacggagtgg cgaattcaat tttggtaaaa gtaaaccaaa ttggcacctt gtctgaaaca
     1561 attgatgccg tcgacttggc gcaccgcaac cgctatacat cggtaatgag ccaccgaagc
     1621 ggcgaaacag aagacagcac catagccgat ttagccgtgg cacttaattg cggacaaata
     1681 aaaaccggct cagcttcgcg cagcgacagg atagccaaat acaaccaatt gctcaggata
     1741 gaagaggagc tgggcgacga agcctactat ccgggtagag gatttagatt ttactgattt
     1801 tttttgatat ataaaaatat gcatctatct gaaatagtta ttatatttgt caggagttta
     1861 tttttatgcc agtttttaaa aaagcgattt ttattatctt agttaatttg atgtgtttaa
     1921 atttaaaagc acaaaattgt tatagttttt acaagttacg tttatcagca acagatacaa
     1981 gtttcgctaa aattgaaaat gcgtttaata aggcaatcaa aaagaacaaa aatgaagcat
     2041 gtctctatgc tgaaaggagt ctgttgtacc gtaaattttt aaaatttgat ttagccttaa
     2101 atgacgtcaa taaagcaata aaattgcaac ctgaaaaata tttattttat gaagataaag
     2161 tagatttgct tttggaaata gatagtatgg cctcggtaaa agaccttatc aacacgatga
     2221 tgatcatgga ttcttctatc cccaatgcgt atgtatttaa ggggaaatac ttcctgcatt
     2281 tagatagcct agagaaagct tttgaggcat ttaataaagc cataacaatt tatgaaagta
     2341 atcatgccaa ttgttgttta atgtgtgaat gttatagagc aaggggaaga ctcaattttg
     2401 cctttcataa attcgatgat gccatttcag atttcactaa agcaatgaaa atggttagag
     2461 ttagcaaaag agataagtat tatctgttga gagccaatgc atatattgct aaagatgatt
     2521 taataaatgc aaacaaggat atagatactg cgattaaatt aaaaccggat tcctttactt
     2581 tagctttttt ctatgcgctt aaaaatgata cactaaaact aaattccata attccttatc
     2641 tgcttgataa aaaaataaat actgaaaagc aagaatatat taaactatat aatatttcat
     2701 gtttatatgc aattacaaat caaacagata aagcatttga atttttagaa aagtcactta
     2761 atgcaggtta taaaaatttt aactggattc aaatagatta tgatatgaag aatatcagca
     2821 aacttccaaa gtttaatgaa ttacttcagc aatatcgtaa gcataccaaa tcagatgaat
     2881 agtatattga ataggtatat cagtttatcc tgctttctat atgcaagact taaagtttaa
     2941 gaataatcgt ttggtgtcca taccaaatga aataaatgtg tatgtctatt tagcagaaca
     3001 ggaaacataa aacaaacccc actgcaactc caaaatagaa aaatacaata agcaagcagc
     3061 actccaactg gtttgttctc tcgcctcatg cgagataatc attggctttt caagcccaac
     3121 gaaaacttag ttgttgtttt ggcggttgta ttttttttat tattctttct tcgtccgttt
     3181 caactttcaa aattattttg tcagatgtca cggtgtcaat ctcaacgcaa tttctaagtc
     3241 cgccaattac atgctcaata cattttgatt tctcaaaatc agttgaaatt atttcatttt
     3301 tattcttcac atcataaata atcagttttt cgttttgctc atgttcattt tctatgtaga
     3361 gcatgacttg ttctttctc
//
LOCUS       DAOQII010000268         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_18219_length_3368_cov_2.985210, whole genome shotgun sequence.
ACCESSION   DAOQII010000268 DAOQII010000000
VERSION     DAOQII010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18219_length_3368_cov_2.985210"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLF48_10690"
     CDS             complement(<1..219)
                     /locus_tag="PLF48_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49839.1"
                     /translation="MKKYIFILLSSVSIFIISCNPDTPEPTTPKLIFKFKFDSTQERL
                     NNFGNLAVMPDGHKAQTPLFNQMSAHYIE"
     gene            complement(304..1221)
                     /locus_tag="PLF48_10695"
     CDS             complement(304..1221)
                     /locus_tag="PLF48_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNF49840.1"
                     /translation="MQTLPHNDLFLADNLDLLARQVVEGFIIGLHKSPYHGFSVEFAE
                     HRLHNAGDSIKDIDWKVFARTEKLYTKKFEEETNLRCQIIIDASSSMYFPEKDSKTKY
                     NKMEFSCLAAASLMHLLRKQRDAVGLTIFGHEILSQTSAKTNALHHQVLMSSLYQLIN
                     QTPQQQKTAAAKSLHQIADTIHKRSMVIIFSDMLDAVDETELFLALQHLKHAKHEVIL
                     FHVLDKKQELDFEFENRPYRFVDLETGEELKLQPNQVKELYIQKMKERKLQLKMKCAQ
                     YKIDLIDVDIQEGFYPVLMSYLVKRSKMV"
     gene            complement(1341..1661)
                     /gene="trxA"
                     /locus_tag="PLF48_10700"
     CDS             complement(1341..1661)
                     /gene="trxA"
                     /locus_tag="PLF48_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNF49841.1"
                     /translation="MALQLTTENFKEKALAENTVALVDFWAEWCGPCRLIGPVVEELA
                     TEYDGKAVIAKVNVDVEGDIAGDYGIMSIPTLLFFKNGQLVDKHVGVAAKSELKKKLD
                     ALIG"
     gene            complement(1832..2131)
                     /locus_tag="PLF48_10705"
     CDS             complement(1832..2131)
                     /locus_tag="PLF48_10705"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNF49842.1"
                     /translation="MANYRNSKFVPLKEVLDDMIQEMRLGTKMHELKIRKFWFETMGS
                     FIANHTSKMYFRQNKLFVFIDSSALRQELFLAREKLRQKLNEYLGEELVQEIIVR"
     gene            complement(2118..3221)
                     /gene="recF"
                     /locus_tag="PLF48_10710"
     CDS             complement(2118..3221)
                     /gene="recF"
                     /locus_tag="PLF48_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNF49843.1"
                     /translation="MQSGQLTIQYLNLVNFKNYKEAAFDFHPKLNIIAGNNGVGKTNL
                     LDAIYYTCVSKSYLSADNLVFRHEADFFRIECDLSHLQSAQKIEIKYPKTGKKELAVQ
                     GVKQDKITDYVGRFPVVMITPDDSELILGSSDNRRRFLDAMLSQLSKAYLSDILLYNK
                     LLQQRNAVLKSFYENRKTDLHLLQAYDEKWVPLGKKIYLARKNGVTALLPVFHQIYQT
                     IFKGEEAVHIQYKSDLEDTDFDLLIKQTLQQDIAAQRSTKGIHTDDCLFSLNTFPLKK
                     TGSQGQQKTFLLSLKLAQYQLMKDKLACSPVLLLDDIFDKLDSSRIQQIIRLVSTENY
                     GQVFFSDTEKQRMEKLFDENSVTSYKIIQLGEL"
BASE COUNT         1031 a          636 c          570 g         1131 t
ORIGIN      
        1 ttcgatataa tgtgcactca tttgattaaa taaaggagtc tgtgctttat gaccatcagg
       61 catcacggcc aaatttccaa aattattcag tctttcttga gtagagtcaa atttaaactt
      121 gaatatcagt ttcggtgttg taggctcagg ggtatccgga ttacaggaaa taataaatat
      181 cgaaacggac gacaacagta taaaaatata ttttttcata atcctcattt atgaattaaa
      241 gataacaatt ttggcagatt tttttcaaat ttaccgtaat atcaatacta taacttgcaa
      301 atgctatacc attttacttc gtttcactaa atacgacatc aataccggat aaaatccttc
      361 ctgaatatca acatcaatta agtcaatttt gtactgtgca catttcattt taagttgcag
      421 ttttctttct ttcatttttt ggatatacaa ttcttttacc tgattaggct gtaactttaa
      481 ttcttcgccg gtttccaaat caacaaaacg ataaggtctg ttttcaaatt caaaatcaag
      541 ttcctgtttt ttatccagca catgaaacaa aataacttca tgtttggcgt gtttaagatg
      601 ttgtaatgcc aaaaagagtt ctgtttcatc aacagcatcc agcatatcgc tgaaaataat
      661 gaccatcgaa cgcttatgta ttgtatctgc tatttgatgc aaacttttgg cagcggctgt
      721 tttttgctgt tgaggtgtct gattgatgag ttgatacaag gaagacatta atacctgatg
      781 atgcagcgca ttggtttttg ccgatgtttg tgataatatt tcgtgcccga aaatagttaa
      841 tcccacagca tctctctgct tgcgcaataa gtgcatcaaa ctggcagccg ccagacagga
      901 aaactccatc ttattatatt tagttttgct gtctttttcg gggaaataca tagacgatga
      961 agcatctatg attatctgac aacgcagatt ggtttcttct tcaaattttt tggtatatag
     1021 tttttccgtt cgggcaaata ctttccagtc gatatctttt atagaatctc ctgcattatg
     1081 caagcgatgt tctgcaaatt ctacagaaaa gccatgataa ggacttttat gcaaaccgat
     1141 gatgaaacct tccaccactt gacgggctaa taagtcgaga ttgtcggcta aaaataggtc
     1201 gttatgcggt agtgtttgca cagatttatt tcatttaaat tagagattga caagttaacg
     1261 gaaaaaacag agtttccgta ttaaaaaaga caaaaaaaat gccgacaaaa gtcggcagta
     1321 tgttgaaatt gagctaattt ttatccgata agggcatcta atttcttttt caattcagac
     1381 ttagcagcta cccctacatg tttgtctact aattgtccgt ttttgaaaaa caataaagtc
     1441 ggaatagaca taatgccata atctccggca atatcacctt ctacatcaac atttacttta
     1501 gcaattacgg cctttccgtc atattcagta gctaattctt ccactaccgg accaattaat
     1561 cggcaaggac cacaccattc cgcccaaaaa tctactaagg ctactgtatt ttctgccaat
     1621 gctttttctt taaaattttc tgttgttaat tgtaatgcca ttttttattt tttttattgt
     1681 gaaatcaaat gctgttccaa agatacgaaa agtgtcattt cggcagtatc agaaaaatca
     1741 atagcacaat agagggaatt tatgggcaat taactaattg attaattagc taattagcta
     1801 attagctaat cgattaattt actcattata atcaacgaac aataatttcc tgcaccagct
     1861 cctctcccag gtactcgttt aatttttgtc tcagtttttc tctggctaaa aataactcct
     1921 gccgcaaagc ggatgaatct ataaagacaa acaatttatt ctggcgaaag tacattttgg
     1981 atgtgtgatt ggcaataaaa ctacccattg tttcaaacca aaatttgcga atcttcagct
     2041 catgcatttt tgtgcccaaa cgcatttcct gaatcatatc atccagcact tctttgagtg
     2101 gaacaaactt actgtttcta taattcgcca agctgaataa ttttatacga agtaacggaa
     2161 ttttcatcaa ataatttttc cattctttgc ttttctgtat cactaaaaaa tacttgcccg
     2221 taattttcgg tactgaccaa acgaatgatt tgctgtattc tgctgctgtc cagcttgtca
     2281 aagatatcgt ccagcaacaa taccggagaa catgccagtt tatctttcat cagttggtac
     2341 tgtgccagct ttagcgaaag taaaaatgtt ttttgctgac cttgtgagcc cgtctttttc
     2401 agtggaaatg tatttaaaga aaataaacaa tcatcggtat gaatgccttt ggtactgcgc
     2461 tgtgcggcaa tatcctgttg aagtgtctgt ttaatgagta agtcaaaatc ggtatcttcc
     2521 aaatccgatt tgtattgaat atgcaccgct tcttcccctt tgaaaatagt ctgatagatt
     2581 tgatgaaaaa caggcaataa tgccgttaca ccgttttttc tggcgaggta aattttctta
     2641 cctaaaggca cccatttttc gtcgtatgcc tgtaagagat gcaaatccgt ttttctgttt
     2701 tcataaaatg atttcaaaac ggcattacgt tgttgcagca acttgttgta cagtaaaata
     2761 tccgataaat acgctttaga taactgcgaa agcatggcat cgaggaaacg gcggcgatta
     2821 tcactgctgc ctaagatgag ttcgctgtca tcaggcgtga tcatcacaac cggaaaacga
     2881 cctacatagt ctgttatttt atcttgtttt actccctgta ctgccagctc tttttttcca
     2941 gttttaggat attttatttc aatcttttga gcagattgca aatgagataa atcgcattcg
     3001 attctaaaga aatcagcctc atgtcgaaac accagattat cggcagataa ataactttta
     3061 gatacgcaag tgtaataaat ggcatcgagc aaattcgttt tgcccacacc gttgttgccg
     3121 gctataatat tgagtttagg gtgaaaatca aaagcagctt ctttataatt tttgaaatta
     3181 accagattaa gatattgaat agtcagctgc ccagattgca tgcaacaaag gtaagcagat
     3241 aaatgagatt gaaaaagttt ggactttgca gattaatgag caggctacat ccgcccgggt
     3301 tgggataacc tttcaattta aaaatagttg attttttatc tataacctcc tacttttaat
     3361 tccacctc
//
LOCUS       DAOQII010000269         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_18230_length_3367_cov_4.448671, whole genome shotgun sequence.
ACCESSION   DAOQII010000269 DAOQII010000000
VERSION     DAOQII010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18230_length_3367_cov_4.448671"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLF48_10715"
     CDS             <1..200
                     /locus_tag="PLF48_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49844.1"
                     /translation="FQQKINNLEQKIIEQNKEKENLNEQIKLVKTTKEVYLSENERAA
                     TKQKLKEFMTEIDDCLAKLVQ"
     gene            213..509
                     /locus_tag="PLF48_10720"
     CDS             213..509
                     /locus_tag="PLF48_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNF49845.1"
                     /translation="MAEHLNINLLIAGRPYKLKVAQDEEGYVRQAAKEINDKIADYQK
                     TLLTRDRQDFLSMIALQATTDVLQNKNKATNANIDTKLDELEIILDKALGLTLF"
     gene            608..2236
                     /gene="rny"
                     /locus_tag="PLF48_10725"
     CDS             608..2236
                     /gene="rny"
                     /locus_tag="PLF48_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNF49846.1"
                     /translation="MDPILLSIISGVVALVIGFIIGKMISKANVDKQIAEAKLIEERA
                     KSIEQKANDLQEKAKAEADLVKKEAQSTAESIKKDKMMEAKEHFLKLKEEHNKEIDLK
                     NKKISESESKLKQTEQSLKDRMANVGKQEKENEDIKADLNKQLEIAQNKRTELEKAQS
                     EHIAKLEQVAKLSADEAKAQLVEQLKGKAQTEALAYVKNAIDEAKLKAGKEAKKIVIQ
                     TIQRTAAESAIENTVSVFNLSSDEQKGQIIGREGRNIRALEAATGVEIIVDDTPEAII
                     ISGYDAYKREIARLSLQRLVTDGRIHPARIEEVVAKTEKQLEQQIMEIGERTVIDLGV
                     HGLHPELIRLVGKMRFRSSYGQNLLQHSREVANLCAIMASELGLNAKMAKRAGLLHDI
                     GKVSDENPELSHALLGAKLCEKFGEQAAIINAVGAHHDEMEMLYLISPIIQACDAISG
                     ARPGARREMMESYMKRIDELEKMAQAYSGVQKAYAIQAGRELRVLVEAEKVDDKKVEE
                     LSFKIAQQIQDEMTYPGQIKVTVIRETRSVSVAK"
     gene            2349..2834
                     /locus_tag="PLF48_10730"
     CDS             2349..2834
                     /locus_tag="PLF48_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNF49847.1"
                     /translation="MKNIMRNKKAKNMQDSETENTLIVSEPRGMYGSTLNFNPLYFRR
                     KIQDGVSFDFYNKLSTQLEFSEENWADILSLSTKSLQRYSKEKEFKFKPIHSEKLLEI
                     AEVVDLGKSILGNLANFNIWMNTPNLALGNLKPKELITDSYGKQMLIDELNRIEHGIF
                     V"
     gene            2834..3295
                     /locus_tag="PLF48_10735"
     CDS             2834..3295
                     /locus_tag="PLF48_10735"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNF49848.1"
                     /translation="MIIYRLSRIKYADTLSGFGAALSGGRWNSKGTEMIYAAENRTLA
                     MSEVFVHLPIQIIPNDYMILSIHVPESIAYDILRIDELPPDWANYPHSNNTQIIGDNF
                     IRKNKLAVLRVPSVITKDEFNFLINPYHQDFHQIKIINKEPFLFNSRLFKS"
BASE COUNT         1312 a          525 c          649 g          881 t
ORIGIN      
        1 tttttcagca aaaaattaat aacttagagc agaaaataat tgaacaaaac aaagaaaaag
       61 aaaatttaaa cgaacaaata aaattggtaa aaacaacaaa agaagtgtat ttatcagaaa
      121 atgagcgagc agcgaccaaa cagaaactga aagaatttat gacagagatt gatgattgtt
      181 tggctaaatt agtacagtaa cttaaacgat acatggcaga gcacttaaat ataaaccttt
      241 taattgcagg acgtccttac aaactgaaag ttgcacaaga tgaggaagga tatgtacgtc
      301 aggcagcaaa agaaataaat gataaaattg ccgattatca aaaaacgttg cttactcgcg
      361 atcgacaaga ttttttatcc atgattgcat tacaagccac tacggatgta ttgcaaaata
      421 aaaataaagc taccaacgcc aatattgaca caaaattgga tgagttggaa atcatactgg
      481 ataaagccct tggcttaacg cttttctgat acaatttcca taagtgttta taagtacagt
      541 ttcgagctgt tgttttttac ccataatttg gtaaactatt ttaatcacta tataaattac
      601 acagtttatg gatcctatac tattaagcat tatatcagga gttgttgcat tggtaatcgg
      661 ttttatcatt ggaaagatga taagcaaagc aaatgttgac aaacaaatag cagaagctaa
      721 gcttatcgaa gaacgtgcaa aatcaataga acaaaaagca aatgaccttc aggaaaaagc
      781 caaagcagaa gcagacttag tcaaaaaaga ggcgcagtca acagcagaat ccatcaaaaa
      841 agataaaatg atggaggcta aagagcattt tctgaaatta aaagaagagc acaataaaga
      901 aattgacctg aaaaacaaga aaatcagtga gtctgaaagc aagctgaaac aaacagagca
      961 atcgctaaaa gaccgaatgg ctaatgtagg taagcaagaa aaagaaaatg aagatattaa
     1021 agccgactta aataagcaat tagaaatagc gcaaaacaaa cgtactgaac ttgaaaaggc
     1081 acaatctgag catattgcca aattagagca agttgccaaa cttagtgctg atgaagcaaa
     1141 ggcacaatta gtagaacagt tgaaaggcaa agcacaaacg gaggccctgg cttatgtgaa
     1201 gaatgctata gacgaagcta aattaaaagc cggaaaagaa gcaaagaaaa ttgttatcca
     1261 aactatacaa cgtactgcag cagagagcgc tattgaaaac acagtatctg tgtttaattt
     1321 gtcgagtgat gagcaaaaag gtcagataat tggtcgtgaa ggacgtaata tcagagcttt
     1381 ggaagcagca accggcgtcg aaattattgt agatgatact ccagaagcca ttattatttc
     1441 gggttatgat gcatataaac gggaaattgc acgtctttcc ttacaaagac ttgtaacgga
     1501 tggacgtatc cacccggcaa gaatagaaga agttgttgct aaaactgaaa aacaacttga
     1561 acagcaaatc atggaaatag gtgagcgcac agttattgac ttaggtgttc acggcttaca
     1621 tccggagctg attcgtttgg taggaaaaat gcgtttccgt tcttcttacg gacaaaactt
     1681 attacaacac tcacgcgagg ttgcaaattt atgtgctatt atggccagcg aactgggatt
     1741 gaatgcaaaa atggccaaac gtgccggatt attacatgat ataggtaaag tatctgatga
     1801 aaatccggaa ctttcgcatg cgcttttagg cgctaagctg tgtgaaaaat ttggtgaaca
     1861 agctgccatc attaatgcag taggggcaca tcacgatgaa atggaaatgc tctatcttat
     1921 ttcgccgatt atacaagctt gtgatgccat ttcaggggca cgccccggag cccgccgtga
     1981 aatgatggaa agctatatga agagaataga cgagttggag aaaatggcgc aagcatattc
     2041 aggagttcag aaagcttatg ctatacaagc gggaagagaa ttgcgagtat tagtagaagc
     2101 agaaaaagta gatgataaga aagtagaaga attatcattt aaaatagctc aacaaataca
     2161 agatgagatg acctatcccg gacagataaa agttaccgtt atcagagaaa ccagaagtgt
     2221 ctctgtagca aaataaataa atgaatcaaa ttttatttta aatgtcttgt gaaaatacaa
     2281 gacatttttt ttgaaaaaaa cttgcaaaac agagacaaaa gacgtaaatt agtggacaaa
     2341 agacggatat gaaaaatata atgcgaaata aaaaagcaaa aaacatgcaa gatagcgaaa
     2401 ctgaaaatac ccttatagtt agtgaacccc gtggaatgta cggaagcaca ctcaacttta
     2461 atccattata tttcagaaga aaaatacaag atggagtaag ttttgatttt tataacaaac
     2521 taagtacaca attagaattt tcagaagaaa actgggcaga tatactatcc ctttctacca
     2581 aatcgcttca gcgatacagc aaagagaaag aatttaaatt taaaccaatc cattctgaaa
     2641 aactattaga aattgcagaa gtagtagatt tgggaaaatc cattttaggc aatttagcta
     2701 attttaatat ctggatgaat accccaaacc ttgctttagg taatttgaaa cccaaagaac
     2761 taattacaga ttcttacgga aaacaaatgt tgatagatga gcttaacaga attgaacacg
     2821 gaattttcgt ttagtgatta tttacagatt atccagaata aaatatgctg atacgctatc
     2881 gggtttcggg gcagcattaa gcggaggtcg gtggaattct aaaggtacgg aaatgattta
     2941 tgctgctgag aaccgaactt tggctatgtc agaagtgttt gttcatctgc caattcaaat
     3001 aataccgaat gattatatga tattaagtat acatgttcct gaaagtatag catatgatat
     3061 tcttagaatt gatgaactcc ctccggattg ggcaaactat cctcattcaa acaacacaca
     3121 aataattgga gataatttta tcagaaaaaa taaattagcc gttttacgag ttccatctgt
     3181 tattacaaaa gatgaattta attttttgat aaatccttat catcaagatt ttcatcagat
     3241 taaaataata aataaggaac cttttttgtt taattcgaga ctattcaaat cttagtaacc
     3301 tgagttcggg ataagcgatt caattaaaaa tagttgattt tttatataga accacctcct
     3361 tttaatt
//
LOCUS       DAOQII010000270         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_18268_length_3363_cov_3.617896, whole genome shotgun sequence.
ACCESSION   DAOQII010000270 DAOQII010000000
VERSION     DAOQII010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18268_length_3363_cov_3.617896"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..821
                     /locus_tag="PLF48_10740"
     CDS             138..821
                     /locus_tag="PLF48_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNF49849.1"
                     /translation="MKTITADLQKNITPRLALELLQQGNSRFVSNLKANRNLLEQVNA
                     TREGQWPFAIILSCIDSRTSVELIFDQGLGDVFSVRIAGNIINTDIIGSMEFACKLAG
                     SKLVVVLGHTKCGAVKGACDHVEMGNLTELLSKLQPAVYAEKQTTTDRNSSNYQFVEN
                     VAEINVKRNVKSIIERSFILEQMVENGEIGVVGAMYNIDTGAVEFYDDVAYIRDSKNP
                     NFSVAELRH"
     gene            complement(823..1797)
                     /gene="meaB"
                     /locus_tag="PLF48_10745"
     CDS             complement(823..1797)
                     /gene="meaB"
                     /locus_tag="PLF48_10745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNF49850.1"
                     /translation="MDTKQQLSSTYYKNGILDGNRFILSKAITLIESTLKQDEEIALT
                     LLDELTLINKTAKRIAITGSPGVGKSTFINAFGKFLSSQQKSVAVLAVDPSSSESRGS
                     ILGDKTRMEDLLNDEHIYIRPTASALHLGGASPRTRDVITLCEAFGFDYILVETVGVG
                     QSETAAKDLTDLFLLLIQPNAGDELQGIKKGIMEMADILIINKSEASNLTQVNHCKAQ
                     LLQAIRLTKNKDIPILAVSSTEHTGMEKVFAEVEQFFQNHTNNGKWLHKREEQKKTAV
                     NENLRLLFDNWLNHHTQDWDSADEQYPQLSARSQALAIFKKILNHVTS"
     gene            2253..3353
                     /locus_tag="PLF48_10750"
     CDS             2253..3353
                     /locus_tag="PLF48_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNF49851.1"
                     /translation="MNKIHAQDVKTSLKFGNGFNVENGDTSFSFNLAAMLQSRIDINK
                     VFDKDFKPTTQLQARRVRLKMKGSFLHQKFDYNLQLSFSPSDVKAANIFLDGWVKYKP
                     IKQFAIQIGQGKLPGDREEIVSAERQFFVDRSTTNSLFKLDRDFGVQLFGNFGKKFIF
                     KPRFSISSGEGKNYTAVDIEHFDYTVRLDFLPTGEFSHGGDLTFQDFDREAKPKVAFG
                     VAYDFNNKPSMSRGQLGGTAVNDTFRKNLHSVYADAILKFKGFTTTGGYVFRKAQDNT
                     KYIAGQSFYATASYLFKKKVEIGARYNRSFAGKYGKEANVNTVNEYAFGLAYYVFKNA
                     LKFQTDYTLLQNKTKTNDQLSGIWRFQMQLAF"
BASE COUNT         1086 a          589 c          629 g         1059 t
ORIGIN      
        1 cttaacttta aagacgttta caatattgaa gacgacgacg attatgtaac tactgtagat
       61 gaaaattcaa gacttcgcaa agagagtttt gatcctgtta ataattaaat aattgacaaa
      121 ataataaata taaatatatg aaaacaatta cagcagattt gcaaaaaaac attacgccaa
      181 gattagcact tgaattatta caacaaggta actctcgctt tgtatcaaat ttgaaagcca
      241 acagaaattt actggaacaa gtaaatgcca caagagaagg tcaatggcca tttgccatca
      301 tactcagttg tattgatagt cgcacctctg tagaacttat tttcgaccaa ggcttaggag
      361 atgttttttc tgttcgtata gccggaaata ttatcaatac agacattatt ggcagcatgg
      421 aatttgcctg taaacttgca ggctctaaat tggttgtggt attgggacat actaaatgcg
      481 gtgcagtaaa aggtgcatgt gaccacgtgg aaatgggaaa cctgaccgag cttttatcaa
      541 aattacaacc ggcagtatat gctgaaaaac aaactacaac agacagaaat tcgagtaact
      601 atcaattcgt tgagaatgtg gctgagataa atgtaaaacg caatgtaaag agcattatag
      661 agcgtagttt tattttagaa caaatggtgg agaatggtga aattggcgta gtgggagcca
      721 tgtataacat agatacgggt gccgtagaat tttatgatga tgtggcatac attcgagata
      781 gcaagaatcc gaatttctcg gtggccgaat taagacatta attcaactcg tgacgtggtt
      841 taaaattttt ttaaaaatag ccaaagcctg acttctggcg gatagttgcg gatattgttc
      901 atctgcacta tcccaatctt gtgtgtggtg attaagccag ttgtcaaaca gcagtcgtaa
      961 attttcattt acggctgttt ttttttgttc ttctcttttg tgcagccatt tgccattatt
     1021 tgtatggttt tggaagaatt gttctacttc agcaaacacc ttttccatac cggtatgttc
     1081 agtagacgat acggccagta taggaatatc tttgttcttt gtcagccgga tagcctgtag
     1141 cagttgtgcc ttgcaatgat tcacttgtgt tagatttgat gcttcagatt tattgatgat
     1201 cagtatatca gccatttcca tgatgccttt ttttatgcct tgcagttcat caccggcatt
     1261 gggttgaata agcaacaaaa ataaatcagt caaatctttc gctgccgttt ctgattgccc
     1321 tacaccaaca gtttctacta aaatataatc aaaaccaaaa gcttcacaaa gtgtaatgac
     1381 atcgcgagtg cggggagaag caccacccaa atgtaaggca gatgccgtag gccggatata
     1441 aatatgctca tcatttagca gatcttccat cctggtttta tcgcctaata tactgcctct
     1501 gctttcactg ctgctgggat ctacagcaag tacggctaca cttttctgct gactactcaa
     1561 aaatttacca aatgcgttga taaatgtact cttacccaca ccgggcgaac cggttatggc
     1621 aattcgtttt gctgttttat ttattagcgt aagttcatcc aacaatgtca gcgcaatttc
     1681 ttcatcctgc ttcagcgtgc tttcaatgag tgttattgcc ttactcaaga taaaacggtt
     1741 tccatccaga attccgtttt tataataggt agacgaaagt tgctgttttg tatccacaca
     1801 caaaaataat cttgttttag cacctgcaca actgctattt ctgatgtagg atttatggaa
     1861 tttgtgtgtt ttttgtgtga attatcaata agaattaaca atacaatgaa aataagtaaa
     1921 cattattact atagtaacgt gttgattttt agcttgttat aataattggg taatttacat
     1981 aatcgttcga catgtttttt gatgattttt ctcataataa agtaatgcat ttcatgctga
     2041 actttgcatc atgattttta caaatctgca cacagataac aaccaaaaca atttattgag
     2101 gaaaaacgtt ccatttaggg aacgacgcac tgattttgcg tcgttttttt atttaaataa
     2161 gtaaatgtaa tattattaaa tcaatcacac accatatgaa accaattact taccagttaa
     2221 aattattatg cctgatattg gcattttcaa gtttgaacaa aattcatgct caagacgtta
     2281 aaaccagttt gaaatttgga aacggattta atgtagaaaa tggggacact tcattttcat
     2341 ttaatctggc agctatgctg caatcaagaa tagatatcaa taaagtgttt gacaaagact
     2401 ttaagcctac cacacaatta caagcacgaa gagtgcgtct aaaaatgaag ggctcgtttt
     2461 tacaccagaa atttgattac aatttgcaat tgagtttttc tccttcagat gtaaaagcgg
     2521 caaatatttt tcttgacgga tgggtgaaat ataaacccat aaaacaattt gccatccaaa
     2581 tcggacaagg aaaattaccc ggagaccgag aagaaattgt ttcagctgaa agacaatttt
     2641 ttgttgacag aagtacaacg aacagtttat ttaagctgga tcgtgacttt ggtgtacaat
     2701 tatttggtaa ctttggtaaa aagttcatct tcaaaccccg tttcagcata tcatcaggtg
     2761 aaggcaaaaa ctacacagcg gtagatatag agcattttga ctataccgtg cgtttagatt
     2821 tcttgccaac gggtgaattt tctcatgggg gcgatttaac atttcaggac tttgacagag
     2881 aagccaaacc aaaagttgct tttggtgttg cttatgactt taataataag ccatccatgt
     2941 caagagggca attgggagga acagccgtga atgatacatt ccgcaaaaac ttacactctg
     3001 tttatgcaga tgcaattctt aagttcaaag gtttcactac taccggaggc tatgtattcc
     3061 gcaaagctca ggataatact aagtatattg ccggacaatc tttttatgca accgcatctt
     3121 atttgttcaa gaaaaaagtg gaaataggtg cacgctacaa ccgttcattt gccggtaaat
     3181 atggcaaaga ggcaaatgta aataccgtaa acgaatatgc atttgggttg gcttattacg
     3241 tttttaaaaa tgcacttaaa tttcaaaccg actatacctt attgcagaat aaaacaaaaa
     3301 ccaatgacca gttatcaggt atctggagat tccagatgca attggctttt taatataaaa
     3361 tat
//
LOCUS       DAOQII010000271         3341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_18488_length_3341_cov_4.870663, whole genome shotgun sequence.
ACCESSION   DAOQII010000271 DAOQII010000000
VERSION     DAOQII010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18488_length_3341_cov_4.870663"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLF48_10755"
     CDS             complement(<1..1092)
                     /locus_tag="PLF48_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF49852.1"
                     /translation="MFKKVAVIGICLLSVGIFFSCKTKPLHKRNVFRLNFYFGLFSLD
                     PAFAKDQGTMWADNQLYNGLVQVDSALNVRPCIAKSWNISKDGLLYEFVLRNDVFFHD
                     NDKFPDGKGRKVTAQDFVYSFGRLIDSNVASTGGWLFNEKVDKQLPFEAVNDTIFRIH
                     LKSPFKPMMGLLTLQYCSVVPKEVVDFYGKDYRKHPCGTGPFKFVRWEENNVLILTKN
                     KSYFEIDSLGNRLPYLDGVRINFVADRGTEYFQFIQHKIDFLSGLDVSYKDKLLTKSG
                     ELREEWKSKINFCKMPYLNTEYLGIAMTNQPNVALRNKKVRQAINYALDKRKMLMYLR
                     NGIGVPAESGMIPAGLPCYAPEAVKGYSYN"
     gene            1422..>3341
                     /locus_tag="PLF48_10760"
     CDS             1422..>3341
                     /locus_tag="PLF48_10760"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF49853.1"
                     /translation="MRLTYLTFIVCLALPFISDAQNNAFHCSQANTLLKMFDKYHYTD
                     LVIDEKVSGEIYDDFVKELDDEAFYLTQNDLKLLDPYRMGIQKDITSNVCKLLEYSTY
                     LLKKKLKFADSLVTAYSKKPIDLSKNENYFTDAAKENTFLANDAALAERWYKAYKYAM
                     LDYIFSHDLKTKSIPEILALEPDIRAKAAAILHRRINFLLQSPEGVDNYVTNIFLNAI
                     ALRYDPHSYFLTQKNMQKFREALSKDQYAYGFSSDEGDNGETKITALVPGGAAWKSNQ
                     LNVGDVLLKVKFENANPVDIAYMSNEELHAMFQSATAKRAEFTVRKANGQIQTVILIK
                     SKIRNDDNIVKGYILEGEKKIGYISLPSFYSNFNGKLPSGCANDVAKELVRLQQEKIE
                     GLILDVRDNGGGSMYEAIDLAGIFIDEGPLGMIKDNQQKISTFKDMNRGAAYSGPLLV
                     LVNNQSASASELLSGILQDYNRAVIVGSTTFGKATVQTTFPMDINYNPNSKTDPSQIF
                     CNQGYTNITYEKFYRVSSKSHQKTGIKPDVEVPQYYNLDSESEATEKYALVSDTVGKT
                     LTYMALPKLPVAALQSNSATRIKNSTKFSTLRNINDSLKIVENQDNSVSLKIDVFKND
                     NNKYSSLKEKIKTLNE"
BASE COUNT         1100 a          606 c          608 g         1027 t
ORIGIN      
        1 attataacta tagcctttta ccgcttccgg agcataacat ggcagacctg ccggaatcat
       61 gccgctctct gccggcacac cgattccatt gcgcaagtac atcagcattt ttcgcttgtc
      121 taaagcataa ttaatagcct gtcttacttt cttgtttcta agtgcaacat ttggctgatt
      181 agtcatggct atacccagat attcggtatt gagataaggc attttacaaa aattaatttt
      241 ggacttccac tcttctcgca actctccgga tttagtcagc aatttatctt tataagaaac
      301 atccaaaccg gataaaaaat ctattttatg ttgtatgaat tgaaaatatt cagtacctct
      361 gtcggctaca aaattgatgc gcacaccatc cagatacggc aacctatttc ctagactgtc
      421 aatctcaaaa tagcttttgt tttttgtcaa aatcaataca ttgttttcct cccagcgaac
      481 aaacttaaat ggaccagtac cgcaaggatg tttcctgtaa tctttaccgt aaaaatcaac
      541 tacttctttt ggcactacag aacaatattg taaagtcaat aaacccatca tgggtttgaa
      601 tggcgatttc agatgtatgc gaaagatagt atcatttaca gcttcaaatg gtagttgctt
      661 atctactttt tcgttaaaca accagccacc ggtagaagct acattgctgt ctatcagtct
      721 gccaaaggaa taaacaaaat cttgtgcggt tactttgcgt cctttgccat ccggaaactt
      781 gtcattatca tgaaagaaca catcatttcg caaaacaaac tcatacaata atccatcttt
      841 gcttatattc caagatttgg ctatacatgg ccttacattc aaggctgaat ctacttgtac
      901 taagccatta tatagctgat tatctgccca catcgtgcct tggtctttcg caaaagccgg
      961 atctaatgag aacagaccga aataaaaatt aagcctgaaa acattacgct tatgcagtgg
     1021 ttttgttttg caagaaaaaa atatgcccac acttagcaag caaataccaa taacagctac
     1081 tttcttgaac ataaaaagtt cattttattt tgatgcaagt tatatgtact tgtggtttaa
     1141 tacaactatg gtttattcac gtttgttatt agctacatgg ttgtatgggt agagagtagt
     1201 gagatatggg taataagtag tgagaaccaa gtgtttagcg tcaatatggt tgttgttcaa
     1261 gctaaaaaaa atattgactg ttttatttct ttttatcaga tgtttattgc agaaatatag
     1321 catccggcta agggtttgat tttttttaaa aaaagtttgg cgctgttatt ttttatttga
     1381 aactaattgc tatattttag cgttataaga agtaataaat tatgcgactt acatatctca
     1441 cttttatcgt atgccttgct ttgcctttta tttctgatgc acagaataat gcatttcatt
     1501 gcagtcaggc aaacactctt ttaaaaatgt tcgataaata tcactatacg gatttggtga
     1561 ttgatgaaaa agtttcgggt gaaatttatg atgacttcgt gaaggagttg gatgatgaag
     1621 ctttttatct tactcaaaat gacttgaagc ttttagatcc ttatcggatg ggtatccaaa
     1681 aagacattac atcaaatgtt tgtaaactct tggaatattc gacttactta ttgaaaaaga
     1741 aattaaaatt tgccgattca ttagttactg cttattctaa aaagccaata gatttatcaa
     1801 agaatgaaaa ttacttcacc gatgctgcca aagaaaatac gtttttagcc aatgatgcag
     1861 cactggcaga gcggtggtat aaagcctata aatatgcgat gctcgactac atcttcagcc
     1921 atgatttaaa aacaaaatca attcctgaaa ttttagcttt agagccggat atccgcgcga
     1981 aggctgccgc cattttacac agaagaataa atttcttgct gcaatctcca gaaggagtag
     2041 acaattatgt taccaatatt tttcttaatg cgatagcctt acgctatgat ccgcattcct
     2101 attttcttac tcaaaaaaat atgcagaaat tcagagaagc cttgtctaaa gatcagtatg
     2161 cttacggatt ttcttcagat gaaggggaca atggtgaaac aaaaataacg gcattagtgc
     2221 cgggtggtgc ggcttggaaa agcaatcaat tgaatgttgg tgatgtgtta cttaaagtga
     2281 aatttgaaaa tgccaatcct gtggatattg cttatatgag taatgaagaa ttacatgcga
     2341 tgtttcaaag tgccactgcc aagcgggcag aatttaccgt cagaaaagca aacggacaaa
     2401 tacaaacagt aatactcata aaatctaaaa tcagaaatga tgacaatatc gtaaaaggct
     2461 atattttaga aggcgaaaag aaaatcgggt atatttcgct tcccagcttc tactctaatt
     2521 ttaacggcaa attaccttcg ggatgtgcta acgacgtggc aaaagaactg gtgagattac
     2581 aacaggaaaa aatagaaggc ctgattcttg atgtgcgaga taatggcgga ggttctatgt
     2641 atgaagccat agacttagcc ggtattttta ttgacgaagg accattaggc atgataaaag
     2701 ataatcagca aaaaatatcc acgtttaaag acatgaacag aggtgctgcc tacagcgggc
     2761 cattgctggt cttggtcaat aatcaaagtg cttccgcttc agaattgctg tcgggtattc
     2821 tgcaagatta taaccgtgca gttatcgtgg gcagtactac atttgggaag gcaactgtac
     2881 aaactacttt ccccatggat atcaattata atcctaactc caaaacggat cccagtcaga
     2941 tattttgcaa tcagggctat accaatatta cttatgaaaa attttatcgc gtaagctcca
     3001 agagtcatca aaaaacaggc attaagccgg atgtagaagt gccccagtat tataatctgg
     3061 atagtgaatc ggaagcaaca gaaaaatatg cactcgtatc tgatacggtg ggcaaaacgc
     3121 ttacttatat ggcattgcct aaattgcccg ttgcagcttt gcagtccaat tctgccacac
     3181 gcattaaaaa cagtactaaa ttttctacac ttcgtaatat caatgattcg ctgaaaatag
     3241 tagaaaatca agataacagt gtgtctctaa aaattgatgt gtttaagaat gataataata
     3301 aatacagtag tctcaaagaa aaaataaaaa cgcttaatga a
//
LOCUS       DAOQII010000272         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_19365_length_3248_cov_3.572502, whole genome shotgun sequence.
ACCESSION   DAOQII010000272 DAOQII010000000
VERSION     DAOQII010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19365_length_3248_cov_3.572502"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="PLF48_10765"
     CDS             <1..1532
                     /locus_tag="PLF48_10765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF49854.1"
                     /translation="LLIQSEELKQSSKLRHIIGAAWWQKWDEWQKSDKSVIALPKKLH
                     AELRPYQHKGFEWICLLGEVNAGACLADDMGLGKTLQTIAFLGREYENDSKARFIVVC
                     PSSLIYNWKNELQKFYPDLDTFIYQGSNRKIADYFESTSPVLITTYATLRNDIDELKT
                     IFWNTIVIDESHNIKSIYALSTKAIYEVLSKHKIALSGTPVMNQTFDLYAQLNYLLPG
                     FLGSQEFFRNEYALPIDRNRSKEKMEALHKLTAPFILRRTKQQVATDLPEKTELTLFC
                     EMGEEQREAYELIKAKARQSVFLNIKEDGLAKSKLNILQAILKLRQVCCAPVLAKDDE
                     IKSNQSIKLDVLMDELLNNLSDNKVLVFSQFKDMLHLIAAELQRNKIPYFHFDGDTAI
                     TERQKMVAAFQEEGNTHRVFLMSLKTGNAGINLTAADYVFLIDPWWNTAIQQQAIDRT
                     HRIGQTKNVFAYKMICKDTIEEKIIAIQSKKEETSSALVVEDENFVKNLSQEDVAFLF
                     S"
     gene            complement(2101..3135)
                     /gene="proB"
                     /locus_tag="PLF48_10770"
     CDS             complement(2101..3135)
                     /gene="proB"
                     /locus_tag="PLF48_10770"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNF49855.1"
                     /translation="MQKPVLVFKIGTSSITNTKGDLNEDVIKSVAHQIALLHKKYAIV
                     MVSSGAVGSGKKYIKNFTGKIEERKAAAAIGNPLLLNKYADAFKKYKIAIAQSLCERG
                     HFSDRDKFLQLRATYHELWENNIIPIANENDVVSSRELKFSDNDELATLLAVGFNAEV
                     LLIATSVPGLLDAEGRLVSKVDKFDSTVFALANKEKSDTGLGGMISKLSCAHLATGMG
                     TKVIIFSASEKQGLQNALKEKTGTVFSPKNVSKTARQKWLASGSLVIGSVQVDEGAHK
                     ALLKRKSLLAVGVSKIVRAFDSDEVFDVQDLKGNTFAVARAKVSSRELEKRQKEKGLQ
                     LAHADEIVLL"
BASE COUNT         1014 a          624 c          630 g          980 t
ORIGIN      
        1 ggctgttgat tcagtctgaa gagcttaaac aatcatctaa attgcgccat atcatcgggg
       61 cggcttggtg gcaaaaatgg gatgaatggc aaaaatcgga taaatctgtt attgcacttc
      121 ccaaaaagct acatgcggaa cttcgccctt atcagcacaa aggatttgaa tggatttgcc
      181 ttttgggcga agtaaatgcg ggagcttgtt tggcagatga catgggtttg ggtaaaaccc
      241 tgcaaaccat tgcattttta ggtcgcgaat atgaaaatga cagtaaagcc cggtttatcg
      301 tggtatgtcc gagctctttg atttataact ggaaaaatga gcttcaaaaa ttttacccgg
      361 atttagacac gtttatttat cagggcagca acagaaagat cgccgattat tttgaatcta
      421 catctcctgt tttaattacg acgtatgcta cattgcgcaa tgatattgat gaactgaaaa
      481 ctattttctg gaataccata gttattgacg agagtcataa tatcaaatcg atatatgccc
      541 tttctaccaa agccatttac gaggttttgt caaaacataa aattgcatta agcggtactc
      601 cggtgatgaa ccagacattt gatttatatg cacagctcaa ttatttatta cccggttttt
      661 taggttcgca agagtttttc aggaatgaat atgcacttcc aatagaccga aacaggagca
      721 aagaaaaaat ggaggctttg cataaattga cagcaccatt tattttgcga agaacaaaac
      781 aacaggtggc taccgactta ccggaaaaaa ccgaattgac cttgttttgt gagatgggcg
      841 aagaacaacg agaagcttat gaattgatta aagcgaaagc ccgacaaagt gtttttctca
      901 atataaaaga ggatggctta gccaagagta agctcaatat tttgcaagct attttaaaac
      961 tgcgccaggt gtgttgtgcg ccagttttgg caaaagatga tgaaataaaa agcaaccaat
     1021 cgataaaact ggatgtgctg atggacgaat tgctcaacaa tttatccgat aacaaagtac
     1081 tggtgttttc acagtttaaa gatatgctgc atttgatagc ggcggaattg caaagaaaca
     1141 agattcctta ttttcatttt gacggcgata cggcaataac agaacggcag aaaatggtag
     1201 ccgcttttca ggaagaaggc aatacccatc gagtattttt aatgagttta aagacgggga
     1261 acgcgggtat aaatttaaca gccgccgatt atgttttcct gatagaccca tggtggaata
     1321 ccgccataca gcagcaagct atagaccgca cccaccgcat aggacaaacc aaaaatgtgt
     1381 ttgcctataa gatgatatgc aaggatacga tagaagaaaa aatcatagcc atacaatcta
     1441 aaaaggaaga aacatcgagt gcactggtcg tagaagacga aaactttgta aaaaacttat
     1501 cccaggaaga cgtggcattt ttgttttctt aacctcgtag gttgggggta tcaaaaaaat
     1561 tatctatttg attatcaata tcttaatatt ttaaccaacc tcgtaggtaa tagcaacaca
     1621 ggcggcatgg ttgaaaatga acaggttgta gaaattaaga gaagatgatg aaacatatag
     1681 tttgttttgt aactttagat acataaataa aaattctttg aaattcttga caatcttgta
     1741 aaaatatttt tgactgaaat agtgtattta gggtcttgaa cttcaaattt gacgttcgta
     1801 acccaaacgt tagggtcttt aaccttaaat ttgacgttgg caaccctaaa attgacagaa
     1861 tgccgtgtga gggatagatg cggaaatact tttgtttcct agcagagtct ctaagcgaag
     1921 ataaagagcg ttgttggact ctgcgtaagt aaggctgcaa cctcaatgaa ctataggtaa
     1981 taaagtgttc ggaggatttt ataaccaaca aaatgaataa taaggtattt ttacggtatg
     2041 gtagtcggca ggtgatatta cctgccaaag gcgaggtact attttcacca acctcgtagg
     2101 ttatagcaac acaatctcgt cggcatgtgc cagctgcagt cctttttctt tttgtcgttt
     2161 ttccagctct ctagaactta ctttcgcgcg agctacagca aatgtatttc ctttcagatc
     2221 ttgtacatca aaaacctcat cactgtcaaa tgctcttaca atcttactta ctcctactgc
     2281 caagaggctc ttacgtttta gcaacgcttt atgtgcacct tcatctactt gcacacttcc
     2341 aattaccaaa cttcctgaag ccagccattt ttgccgagcg gttttactta catttttggg
     2401 actaaataca gttcctgttt tttctttcag ggcattttgc aagccttgtt tctcgcttgc
     2461 agaaaagata ataacttttg ttcccattcc tgtagccagg tgtgcacagc ttaatttact
     2521 tatcatgccg cctaatcccg tatctgattt ctccttgttg gctaatgcaa aaacagtaga
     2581 atcaaattta tctacttttg aaacaagcct gccttccgca tccaacaaac ctggaacact
     2641 cgtagcaatc agcaatactt ctgcattgaa gcccacggca agcaaagtag caagttcatc
     2701 attatccgaa aatttgagct cccgcgaact cactacatcg ttttcattgg cgataggaat
     2761 gatattattt tcccagagtt catgataggt tgcccgcagt tgcagaaact tatcacggtc
     2821 agaaaaatgc ccgcgctcgc acaaactttg agcaatagca attttgtatt tcttaaaagc
     2881 atccgcatat ttattcagca gtaaagggtt gcctatggca gcagctgctt tgcgttcttc
     2941 tatttttccg gtgaaatttt tgatatactt tttgcccgaa cccacagctc cggaagacac
     3001 catcacgata gcatattttt tatgcagtaa tgcaatttga tgcgccacag attttatcac
     3061 gtcttcattc aaatctcctt tggtattcgt aattgacgaa gtcccaattt taaaaacaag
     3121 taccggtttc tgcattttct ttaagtttat ccaaagtaat attatttttg gaagccattg
     3181 cagtttttag tttaataaat ctttaatgat tgacctaaaa aatatcatac aacagtttaa
     3241 tttattgc
//
LOCUS       DAOQII010000273         3214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_19689_length_3214_cov_4.581830, whole genome shotgun sequence.
ACCESSION   DAOQII010000273 DAOQII010000000
VERSION     DAOQII010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19689_length_3214_cov_4.581830"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..2018
                     /locus_tag="PLF48_10775"
     CDS             342..2018
                     /locus_tag="PLF48_10775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNF49856.1"
                     /translation="MRFLLTTLNSKYIHPNLAIRLLYHLNKDKADLSWQEFTIKEDKD
                     AIANFCADFDVVCFSCYIWNIMQTLEVCTLLKQKKSGIKILLGGPEVSYEWENVISLS
                     FIDFIITGEGELPFSKFVEQYPNITNIPNLVSKNDGKINYTEKSETFDLTQYADCNPY
                     QYDDLATLKTKVLYIETSRGCPYKCEFCLASLDNKVRYLPLETIKSQLLFLMQHGRVI
                     KFLDRTFNIKRDFALEIFQFILDHHQAGNVFQFEITADILHPDIIRFIQEKVPKNLFR
                     FEIGIQSVNTQANLAVSRKQNFDKTKDIIKQIENKIELHLDLIVGLPHDYFEDIHKGF
                     NEVFALFAPELQLGFLKLLKGTPVREKYTEYNFVYDALPPYQLIESKFLSKKELQDIL
                     ILEEALEIYWNKKRIIHTLKYVSSYLDAFEFLWQLGLYYEQKMGNQSVTQKAIYQTAY
                     YFILENFETDQLLQQLFVIDYLLQYKVKPETDWLSQSLEKWNFSQEKSTNRHQDKFRS
                     IAFDIPFDWEKFIDMHQIQSTAGRIEIRYQNHDVFLSSYIRNNNINTAFT"
     gene            2132..2803
                     /locus_tag="PLF48_10780"
     CDS             2132..2803
                     /locus_tag="PLF48_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNF49857.1"
                     /translation="MNWKYYLSSPVHIIYFLISLVILLFAAKYCKTYIAEFTQRPGML
                     LHDPLLAQFRPHDFSNIIFVLFHLGIVLCFASFYDDPRGLSVAFQAYALLMLFRTITI
                     FLWPLQAPVDMIFLQDPFAEVYMHQNGHRVVNDLFFSGHVSSSFLFFIVAKGKYTKRI
                     LLFITVTVAIMILWQKVHYTIDVLAAPFFSLLAVKSVSFINAKVHAAWVPPIEVIRQE
                     EVISA"
     gene            complement(2800..>3214)
                     /locus_tag="PLF48_10785"
     CDS             complement(2800..>3214)
                     /locus_tag="PLF48_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HNF49858.1"
                     /translation="GAPVSLTMPLINKVYEFKDGVVKEFIGDIEAFFEERRLQDFRDV
                     ELSQQAVTESTPKNTISSEQKTVNTSDKKIKNQISKLEQEIEDKETQINVLELKIAEL
                     SAEGKYEEQIVKQLGENKSQLNKLMEDWEKLSEQV"
BASE COUNT          984 a          570 c          547 g         1113 t
ORIGIN      
        1 atgattagtt gataatatct cattaaacta agttaagaaa tctccttatt tttttagagt
       61 gctaaatact tatttttgtt taagtcgtaa ctttttccgt atttttagct tgcccagtat
      121 gcacgggatt cctatctccc tactcacttc gtagcaggcg ggcgttcggg atgacgtgtt
      181 tttattcagt ggatttctcc ctacggtcga aatgacgaaa attgaaatag tacaactggt
      241 aatcatgaat agctttttta gtttggtgaa cactaaaggt gaagtaaatg cagcctcttt
      301 tatgcgagcc tcttcttgga aaatatttta cctttgcgct tatgcgtttc ttgctgacta
      361 ctttaaattc taaatatatc cacccgaatc ttgccattcg tttattatat catttgaata
      421 aagataaagc agatttaagt tggcaagaat tcaccataaa agaagataag gatgccattg
      481 caaacttttg cgcggatttt gacgtggttt gcttcagttg ctatatctgg aatatcatgc
      541 agactttaga agtatgtaca ctactcaaac aaaaaaaatc cgggattaaa attctgcttg
      601 gcggacctga agtaagctat gaatgggaaa atgttatctc acttagtttt atcgatttta
      661 ttatcaccgg tgaaggagaa cttcctttct cgaaatttgt agaacaatat ccgaacatca
      721 caaacattcc gaatcttgtt tctaaaaatg atggaaaaat aaactatact gaaaaaagcg
      781 aaacatttga cttgacgcaa tatgctgatt gcaatcctta tcagtatgat gaccttgcga
      841 cattaaaaac aaaagtgctc tatattgaaa cctctcgcgg ttgtccgtat aaatgtgaat
      901 tttgtctggc aagtttggat aacaaagttc gttacttacc tttggaaact ataaaatcac
      961 aattgttgtt tttgatgcaa catggcaggg tgattaaatt tttagaccgt acatttaata
     1021 tcaaacgtga ttttgcgctg gaaatttttc agtttatttt agaccatcat caagcaggca
     1081 atgtctttca atttgaaata actgccgaca ttttacatcc ggatattatt cgatttatac
     1141 aggaaaaagt gccgaaaaat ttatttcgtt ttgaaattgg cattcaaagc gtaaacacgc
     1201 aggcaaactt agctgtaagc cgaaaacaaa atttcgataa gactaaagac attattaaac
     1261 aaatagaaaa taagatagaa ttgcatctgg atttgattgt cggtttgccg catgattatt
     1321 ttgaagatat tcataaaggg ttcaatgaag tatttgcctt gtttgcaccc gaattgcagc
     1381 tcggtttttt gaaattatta aaaggaacgc ctgttagaga aaaatatacg gaatacaatt
     1441 ttgtttacga tgctttgcca ccttatcaac ttatagaaag taaattttta tccaaaaaag
     1501 aattgcagga tattctcatc ttggaagaag ctctggagat atactggaat aaaaaaagaa
     1561 taattcatac gctgaaatat gtttcatctt atctggatgc atttgaattc ttatggcaac
     1621 ttgggttata ttatgagcaa aaaatgggaa atcagtctgt aacccaaaaa gccatttatc
     1681 aaactgccta ttattttatt ctggaaaact ttgaaactga ccaactgttg cagcagcttt
     1741 ttgttattga ttatctttta caatacaagg taaaacccga aacagactgg ttaagtcaaa
     1801 gtttggaaaa gtggaatttc agtcaggaaa aatcgaccaa cagacatcag gataaattca
     1861 ggagtatcgc ttttgacatc ccgtttgact gggagaagtt tatagatatg catcaaattc
     1921 aatctacagc cggacgtata gaaatacgct atcaaaatca tgatgtattt ctatcatcat
     1981 acatacgaaa taacaatatt aatactgcat ttacttaaat tttggttaaa aacaactatt
     2041 tgtaattttt atgtggcgaa tgtgaaaatt ttgaaatatt tttgtgcttt attgagaacc
     2101 tgaaatggct gcaaaaatca aaaatggact tttgaattgg aagtattatt tatcttctcc
     2161 agtacatatt atctactttc ttatttcttt ggttattctt ttatttgctg caaaatattg
     2221 caaaacttat atcgcagaat ttacgcaacg tccgggtatg ctactgcacg atccactact
     2281 ggcgcagttc agaccacatg acttttccaa tatcatattc gtgctatttc atttaggaat
     2341 cgtcttgtgc tttgcatcct tttatgacga tccgagaggt ttgtctgtcg cctttcaggc
     2401 atatgccttg ctgatgctgt ttcgtaccat caccatcttt ttatggcctt tgcaagcacc
     2461 tgtagatatg atttttttac aagatccctt tgcagaagtt tacatgcatc aaaatggaca
     2521 cagagtagta aatgacttat ttttctccgg acatgtatcc tccagcttct tgttttttat
     2581 cgtggcaaaa ggcaagtaca ccaaacgcat tttgttgttt attaccgtta cagtagccat
     2641 aatgatttta tggcaaaaag tgcattatac aattgacgta ttagcagccc ctttcttctc
     2701 ccttttagct gtgaaatcag tatcttttat caatgccaaa gtacatgcag cctgggttcc
     2761 accaatagaa gtaattcgtc aggaagaggt gatttctgct taaacttgct cactcaattt
     2821 ttcccaatct tccatcagct tatttaactg agatttattt tcaccgagtt gtttcactat
     2881 ttgttcttca tattttcctt cagctgaaag ctcggcaatc tttagttcca atacatttat
     2941 ctgtgtttct ttgtcttcaa tttcctgctc caatttactt atctgatttt tgattttttt
     3001 gtctgaagta ttcaccgttt tctgttctga actgattgta ttcttaggag tgctttcagt
     3061 tacagcctgt tggcttagct ccacatcacg gaaatcctgc aaacgacgtt cttcaaaaaa
     3121 ggcttcgata tctccgataa attctttgac aacaccatct ttaaattcat acactttatt
     3181 aattaacggc atagtgagag aaacaggtgc gcct
//
LOCUS       DAOQII010000274         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_19771_length_3205_cov_4.425397, whole genome shotgun sequence.
ACCESSION   DAOQII010000274 DAOQII010000000
VERSION     DAOQII010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19771_length_3205_cov_4.425397"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLF48_10790"
     CDS             complement(<1..397)
                     /locus_tag="PLF48_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNF49859.1"
                     /translation="MTTKSAIDFKLPYKVKDISLAAWGRKEIELAEAEMPGLMALRAE
                     YGRTKPLQGARIAGCLHMTIQTAVLIETLVHLGAEVTWSSCNIFSTQDHAAAAIAAAG
                     IPVFAWKGLTAEEFDWCIEQTLFFGSPDRP"
     gene            528..977
                     /locus_tag="PLF48_10795"
     CDS             528..977
                     /locus_tag="PLF48_10795"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF49860.1"
                     /translation="MTFEYSSFVRCTKDEAWELISNIERRPEWIHFQEKCYWLEMKPG
                     MVGSTYQEVEVFLGFHINVKYEVTAWEPYRKMTSHTKMPPFHQTVEVYMEERVDGCYT
                     RLVIHADMGILGLIPKFIIKNKVNELVNPMVDIFIDILENNSVLKNK"
     gene            1014..1640
                     /locus_tag="PLF48_10800"
     CDS             1014..1640
                     /locus_tag="PLF48_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNF49861.1"
                     /translation="MKDYYQILEISKTADAVAIKKAYHSLAKLYHPDVNKGNIFFENK
                     FKEINEAYAILSDTNKKWTYDLMLNQPYVSAATSPKEESKKTYYPKGFNINKYRENER
                     KAQFILNIVKVFVFAFFAGLIVFFSINDFPRNRSDDVQETNELKFHDDTLLLNPNSDS
                     TLNEHHQLSKEEFYRILDVEYKDSGDSTLWKSNEDSLYHVLDSMVNSH"
     gene            complement(1645..2358)
                     /locus_tag="PLF48_10805"
     CDS             complement(1645..2358)
                     /locus_tag="PLF48_10805"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNF49862.1"
                     /translation="MTKLSVNINKIALIRNSRGGNYPDLLQVAKDCERFGADGITVHP
                     RPDERHVRYADLQPLKNIVTTEFNIEGYPDEKFMQKVLEVKPHQCTLVPDAPGQITSD
                     HGWNAKQNKEMLTDVISTLQKENIRVSLFIDADKDMIAEAKNCGADRIEFYTGPYAHD
                     FSDDKMNAIMPLLSACEFAVSCGLGINAGHDLNLQNLAYFKQHMPQLDEVSIGHALFS
                     DAIYLGLENTIKLYKCCLQ"
     gene            2441..3205
                     /locus_tag="PLF48_10810"
     CDS             2441..3205
                     /locus_tag="PLF48_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF49863.1"
                     /translation="MNLYFKKFGDSPSHLIILHGLLGSSDNWQTIAKNLSGKFSVYVL
                     DARNHGRSPHDNVMTYELMVIDLLHFMLQEKIDKATLIGHSMGGKTAMLFALNYPEKT
                     DKLIVVDIAPKQYDGGHEEILFAMAEAPLQVTQDRKDIDVFLQKRIPDFGIRQFILKN
                     LTRDEYGKLVWRSNVEGIIHAYRGLMGFPNVETVFDGETYFIKGEESDYLLENDEAAI
                     RYLFPNASIIAIPKAGHWVHADQPELFLQTINLLLK"
BASE COUNT         1005 a          546 c          631 g         1023 t
ORIGIN      
        1 atggacgatc aggtgaaccg aaaaataaag tttgctcaat acaccagtca aattcttcag
       61 ctgtcaagcc tttccatgca aaaaccggaa ttcctgctgc agctattgcc gctgctgcgt
      121 ggtcttgagt agagaaaata ttacaagaag accaggttac ttcggcacca aggtgaacta
      181 gtgtttctat taataccgca gtttggatag tcatgtgcaa acagcctgca atacgtgcac
      241 cctgtaaagg tttagtacgt ccgtattctg cgcgcaatgc cattaagccc ggcatttctg
      301 cttctgccaa ttcaatttct tttctgcccc aggctgccag actaatgtct tttactttgt
      361 aaggcaattt aaagtcgatt gctgattttg tagtcatgtt taatttttta tagtgcaaaa
      421 ataaaatcct cttccaattt ggaagttgtc ctatggcaat aaatgaaatg caaaggatat
      481 ttttataaaa tggattaaaa tgtgaacaga tttcttattt ttatttcatg acttttgaat
      541 atagcagttt tgttcgttgt accaaggatg aagcttggga gctcatctcc aatatagagc
      601 gcagaccgga gtggatacat tttcaggaga aatgttattg gctggaaatg aaacccggta
      661 tggtaggctc tacttatcag gaagtagaag tgtttttagg ttttcatatc aacgtgaaat
      721 atgaagtaac ggcttgggag ccttacagaa agatgaccag ccacacgaaa atgccccctt
      781 tccatcaaac cgtagaagta tatatggaag aacgggtaga tggttgttat acacgtttgg
      841 tgatacatgc cgatatgggt attttgggct tgatacctaa gtttatcatt aaaaataaag
      901 taaatgaatt ggtaaatcct atggtagata tttttattga tattttggag aataattcag
      961 tgttgaaaaa caaatagtac ttattgccgt tagttcttta gcttgttttt agattgaaag
     1021 attattatca aatattagaa atatccaaaa ctgcagatgc tgttgccata aaaaaagcgt
     1081 atcacagtct tgcaaaattg tatcatcccg atgtcaataa aggcaatatt ttttttgaaa
     1141 ataaatttaa agaaatcaat gaagcttatg ccatactttc cgatactaac aaaaaatgga
     1201 catacgattt gatgttaaac cagccttatg tttctgcagc tacgtctcca aaagaagaat
     1261 ccaaaaaaac gtattaccca aaaggtttta atataaataa gtaccgggaa aatgaacgca
     1321 aggcacagtt tattctgaat attgtaaaag tattcgtatt tgcttttttt gcaggtttga
     1381 ttgtgttttt ttcaattaat gattttccaa gaaacagaag tgatgatgta caagaaacga
     1441 acgaattgaa atttcatgac gatactttac tgttaaatcc aaattcggac tcaactttga
     1501 atgagcacca tcaacttagc aaagaagaat tttacagaat tttggatgtg gaatataaag
     1561 acagcggaga ctccacactt tggaaatcta atgaagattc actttatcac gtattggatt
     1621 cgatggtgaa tagtcattaa tattctattg caagcaacat ttatacagct ttatcgtatt
     1681 ttccaatccc aaataaatgg catccgaaaa aagtgcgtgt ccgatggaaa cttcatccaa
     1741 ctgtggcatg tgttgcttga aataagcaag attttgcaag tttaaatcat gtcccgcatt
     1801 gatgcctaag ccacaggata cagcaaattc acaggcagac agtaaaggca ttatggcatt
     1861 cattttatca tcagaaaaat catgtgcata aggtccggta taaaattcaa tccgatccgc
     1921 tccacagttt tttgcttctg caatcatatc cttatctgca tcaataaata aggatactct
     1981 gatattttct ttttggagag tagaaataac atctgtcagc atctccttgt tttgtttggc
     2041 attccagcca tggtcagaag taatttgtcc gggtgcatcc ggcaccaaag tgcattgatg
     2101 tggttttact tccaatactt tttgcatgaa cttttcatcg ggataacctt cgatattaaa
     2161 ctcggtagtc actatgtttt ttagtggttg taaatctgca tagcgcacat ggcgttcatc
     2221 cggacgagga tgtacggtga tgccgtcagc cccaaatcgc tcacaatctt ttgccacttg
     2281 cagcagatcc ggatagttgc caccccgcga attgcggatt aaggctattt tgtttatatt
     2341 gacagatagt ttggtcatgc tttatcttga tttgattttt caaaattaca atattttagt
     2401 acgtttgcat gtattggaac aaattcatac aaagtaaaac ttgaatttat actttaaaaa
     2461 atttggtgat tcgcctagtc atttaattat tctgcatggc ttgctgggct cgtcggataa
     2521 ctggcaaaca attgccaaaa atctatccgg caagttttcc gtttacgttc ttgatgcacg
     2581 taatcatggt agatctccac acgataatgt catgacatat gagttgatgg taattgattt
     2641 actacatttt atgttgcaag aaaaaataga taaggcaaca ctaattgggc attctatggg
     2701 cggaaagact gccatgcttt ttgcgttgaa ttatcccgaa aaaacggata aactgattgt
     2761 tgtggatata gctccaaaac agtatgacgg aggtcatgaa gaaatattgt ttgctatggc
     2821 agaagcaccc ttgcaggtta cacaagaccg taaagacatt gatgtatttc tgcaaaaacg
     2881 catccctgat tttggtattc gtcagtttat tttgaaaaat ttgacaagag atgaatatgg
     2941 aaaattagtt tggagaagca atgtcgaggg tatcattcat gcttatcggg gtttgatggg
     3001 atttccgaac gtggagactg tttttgatgg agaaacttat tttataaaag gggaggagtc
     3061 tgattatttg ttagaaaatg acgaagccgc aataagatat ttatttccaa atgcgtcaat
     3121 tattgctata ccaaaagcag ggcattgggt acatgccgat caacccgaat tatttttgca
     3181 gactataaat ttgctgctga aatga
//
LOCUS       DAOQII010000275         3198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_19836_length_3198_cov_4.316577, whole genome shotgun sequence.
ACCESSION   DAOQII010000275 DAOQII010000000
VERSION     DAOQII010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19836_length_3198_cov_4.316577"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="PLF48_10815"
     CDS             complement(<1..1741)
                     /locus_tag="PLF48_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNF49864.1"
                     /translation="MIYNFIEIEEQARQKWQYDNKEKSVYKVENDISKPKYYVLDMFP
                     YPSGAGLHVGHPLGYIFSDIIARYKRMQGFNVLHPMGFDAFGLPAEQYAIQTGQHPAI
                     TTDNNIETYRMQLRKIGFCYDWCREVITCEPSYYKWTQWIFLQLFNSIYCTENQCAYP
                     ISVLIKHFEEHGNVNNTAYTNAEVPKFTAEEWNQFDALKKQEILMDYRLAFQKDSWVN
                     WCPALGTVLANDEIVNGVSERGGHPVEKKKMSQWFLRITAYAERLLAGLDTLDWTDAM
                     KEMQRNWIGKSKGAMVSFPLKSHDDKIEVFTTRVDTIFGVSFLVIAPEHELVKKITTP
                     EYTDAVNSYIKQAGAKSEIERQAEVKETSGQFTGAYVIHPFTKVEIPVYIADYVLAGY
                     GTGAVMAVPSGDQRDWLFAEKYELPIIPILDAQVNIHIEADPTKEGLYINSGFINGMN
                     YAEATDALIKRMEEEGIGYGKIQYKMRDAGYSRQRYWGEPFPVYYDEYGLIHTVDNLP
                     VTLPEVKSYKPTGTGESPLATVGDWVHQQGIDCKMETDTMPGYAGSSWYFLRYMDPHN
                     EQALVDFNIQDYWK"
     gene            1864..2214
                     /locus_tag="PLF48_10820"
     CDS             1864..2214
                     /locus_tag="PLF48_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNF49865.1"
                     /translation="MSSYPVCPKCKSEYTYPSDDLMICPDCFYEWNPEEEKAEATFQE
                     DVLKVVDANGNELQNGDTVVVIKDLPVKGAAKPIKAGTKVKNIRLTDGDHNIDCKIEG
                     FGALALKSIFVKKA"
     gene            complement(2229..2789)
                     /locus_tag="PLF48_10825"
     CDS             complement(2229..2789)
                     /locus_tag="PLF48_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF49866.1"
                     /translation="MAATETLRIPLGYTAPDFQLLDTVSDKVLSLQQLKGNAATVVMF
                     ICNHCPYVKHVNKQIVQLANDYQPQGVAFIAISSNDVENYPDDAPDKMKEIAKEEAYP
                     FPYLYDETQDTAKAYFAACTPDFSIFDADLKCVYRGQLDDSRPKNEIASDGKDIRTAL
                     DNLLQGLPVAERQKPSLGCNIKWKTA"
     gene            complement(2934..>3198)
                     /locus_tag="PLF48_10830"
     CDS             complement(2934..>3198)
                     /locus_tag="PLF48_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNF49867.1"
                     /translation="IPERFEATALKEKHKYAYFPFGGGPRLCIGNNFALQEMQIVLIL
                     FMQHFDFQIDEQFIPDMEALVTLRQKNALYAKIRNKEEVKINK"
BASE COUNT          955 a          649 c          542 g         1052 t
ORIGIN      
        1 ctttccaata atcttgtatg ttaaaatcta caagtgcttg ttcattatgc ggatccatat
       61 agcgcaaaaa ataccatgaa cttcccgcat atcccggcat agtatcagtt tccattttgc
      121 aatctattcc ttgttgatgc acccaatcac caacagtagc caacgggctt tcgcctgttc
      181 cggtaggctt ataacttttt acttcgggca gggtaaccgg taaattatct accgtatgaa
      241 tcaaaccata ttcatcatag taaacaggaa aaggttcgcc ccaataacgc tgacgagagt
      301 aacccgcatc acgcatttta tactggattt ttccatagcc tatcccctct tcttccatac
      361 gtttgattaa ggcatctgtg gcttccgcat agttcattcc attgataaat ccggaattaa
      421 tatacaaacc ttcttttgta gggtcagctt caatatgaat attgacttgt gcatctaata
      481 ttggaataat aggcaactca tatttttcag caaaaagcca gtcgcgctga tctccgctcg
      541 gcactgccat caccgcaccg gttccatatc ctgctaagac ataatcagct atataaaccg
      601 gaatttctac ttttgtaaaa gggtgaatga cataggcacc tgtaaactgt ccacttgttt
      661 ctttcacttc cgcttgtctt tctatttccg atttagctcc tgcttgttta atatatgaat
      721 ttactgcatc agtatattca ggtgtggtaa tttttttaac cagttcatgt tcaggtgcta
      781 ttactaaaaa gctaacgcca aaaatcgtat ctactctagt ggtaaatact tctattttat
      841 catcatggct ttttaagggg aaagacacca ttgcaccttt ggattttcct atccagttgc
      901 gctgcatttc tttcatggca tctgtccaat ccagcgtatc taagccggcc aataagcgtt
      961 ctgcataagc ggtgatacgc aaaaaccact gcgacatttt tttcttttct accggatgcc
     1021 cgccacgttc tgacacccca ttcacaatct cgtcatttgc taatactgta cccaaagccg
     1081 ggcaccagtt tacccaagaa tctttttgaa atgccagacg gtaatccatc aaaatttctt
     1141 gttttttcaa tgcatcaaat tgattccatt cctctgcagt aaattttggc acttcagcat
     1201 tcgtataggc tgtattattg acattgccat gttcttcaaa atgtttgatt aaaactgaaa
     1261 tcggatatgc acattggttt tcagtacaat aaatggaatt aaataactgt agaaagatcc
     1321 attgtgtcca tttgtagtaa gatggttcgc aggtaattac ctctctgcac cagtcataac
     1381 aaaagcctat tttgcgcaat tgcatgcggt aggtttcaat attattatct gtagtaatgg
     1441 caggatgttg cccggtttga attgcatatt gttctgccgg caatccaaaa gcatcaaagc
     1501 ccataggatg caatacatta aatccttgca tgcgtttgta acgtgcaatg atatcagaaa
     1561 agatataacc cagcggatgc cccacatgca aaccggcacc actaggatat ggaaacatat
     1621 ccaagacata atacttaggt ttggaaatat cgttttctac tttgtaaacc gatttctctt
     1681 tgttgtcata ctgccatttt tggcgggcct gctcttctat ttcgataaaa ttgtaaatca
     1741 tacaacaaaa ataaaaaaag aagcatctac ttgaattata gcataactaa tttgacaatt
     1801 agataattag agcagctgat aatgcgctga taattttata gttttgcagt caaattaata
     1861 cttatgtcaa gctatcctgt atgcccgaaa tgcaaaagtg aatatactta cccgagcgat
     1921 gatttaatga tttgtcctga ttgtttttat gaatggaatc cggaagaaga aaaagcagaa
     1981 gcaacgtttc aggaagatgt attaaaagta gtagatgcca acggcaatga gcttcaaaat
     2041 ggcgatacgg ttgttgttat caaagactta cctgtgaaag gtgcagccaa gccgataaaa
     2101 gccggcacta aagtaaaaaa cattagactt acagacggcg accataatat agactgcaag
     2161 attgagggat ttggtgcttt ggcacttaaa tctatatttg tcaaaaaagc atagtggcag
     2221 aaaagcgact aggctgtttt ccatttaata ttacaaccta aactcggttt ttgtctttct
     2281 gcaacaggca gaccttgtaa taaattatcc aatgcagtcc tgatatcttt accatccgat
     2341 gcaatttcat tttttggtct tgaatcgtcc agttgaccgc gatatacaca ttttaaatct
     2401 gcatcaaaaa tactgaaatc aggtgtacaa gctgcaaaat aggctttagc cgtatcttga
     2461 gtttcatcat acagatatgg aaacggatat gcttcttctt ttgcaatttc tttcattttg
     2521 tcaggtgcat catccggata attttctaca tcatttgaag aaattgcaat aaatgccact
     2581 ccttgaggct gatagtcatt tgccaattgc acaatttgtt tgtttacatg cttcacataa
     2641 ggacaatgat tacagataaa catcacgaca gttgcagcat ttcctttcaa ttgttgtaaa
     2701 gaaagtactt tatcactaac cgtgtctaaa agttgaaagt caggtgctgt atagcccaaa
     2761 ggtattcgca gagtttctgt tgcagccata ttttttattt taaatgttga atgattaatt
     2821 tttaatggtg attgattagt gcttgaggtc tgatagttaa ttatactttt tataaaaaaa
     2881 tccctgaata aatttcctct tcttactact tactccttat tacttaatac ttattactta
     2941 ttaattttca cttcttcctt attccttatt ttggcataca atgcattttt ttgtcttagc
     3001 gtaactagtg cttccatatc cggtataaat tgttcatcta tttggaaatc aaaatgctgc
     3061 ataaacagaa tcaacacaat ttgcatttcc tgtaaagcaa aattattgcc tatacacaat
     3121 ctcggacctc cgccaaaagg gaaataagca tacttgtgtt tttcttttaa cgctgtcgcc
     3181 tcaaatcgtt ccggaata
//
LOCUS       DAOQII010000276         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_19945_length_3187_cov_4.146552, whole genome shotgun sequence.
ACCESSION   DAOQII010000276 DAOQII010000000
VERSION     DAOQII010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19945_length_3187_cov_4.146552"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLF48_10835"
     CDS             <1..297
                     /locus_tag="PLF48_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49868.1"
                     /translation="LEGNHTEIANNIKGDLQSGTVSEKSLFNSSYFAKIGINNTYNSW
                     TNEAFNRAKKGVKTSSKLLIGTVITQNNSDVSSAKYTSTSKPKKKKCIKKKGKK"
     gene            complement(302..1243)
                     /locus_tag="PLF48_10840"
     CDS             complement(302..1243)
                     /locus_tag="PLF48_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNF49869.1"
                     /translation="MKNYLSLVKFSHTLFALPFAMIGFALGITKLRESANNNSLIDSF
                     HPALLKHVYQNHPLAFIMLYKLILVLICMVTARNAAMAFNRYIDRKYDERNPRTAIRE
                     IPSGIITPKNALGFVIINCLIFIVATYFINPLCFYLSFVALAVILGYSLTKRFTFLCH
                     LILGVGLALAPIGAYLAVTAKFDIIPLFFSFAVFFWVSGFDILYALQDEQFDKENQLF
                     SIPAYFGQDKAKWISRTLHFCSFLCLLIVGIFYPFGWLYWIGFAIFTALLIYQHSLVI
                     RFGLQKIDLAFFTTNGIASVLFSLFVILDLLRKNNIS"
     gene            complement(1247..1762)
                     /locus_tag="PLF48_10845"
     CDS             complement(1247..1762)
                     /locus_tag="PLF48_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF49870.1"
                     /translation="MKIAGYGLIISAFIALMFFQCKQKDTSGNVEVQHFNHDSIVQND
                     PTIKRADSMARVTEADDEQRLANATSIKFDKEVYDFGECTEGDVITKEVEFTNTGKKP
                     LVIENAFGSCGCTVPTYDKEPVQPGKKGKVKIQFDSKHKPGHNTKSVNIQANTNPPVT
                     SITFSVTVKEK"
     gene            complement(1841..>3187)
                     /locus_tag="PLF48_10850"
     CDS             complement(1841..>3187)
                     /locus_tag="PLF48_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNF49871.1"
                     /translation="VEMLQIKGIGPKKIRQLWKELDIENIGELEYACNKNRLLELKGF
                     GKKTQENILQQIHFIQQNANKFRWAQLELIANEIVDALQEEFPQQQISFCGDYRRKNI
                     ILEQVDFLVDITDAEIQTQLQEKYKDYPVAFHFSTPQFFYLELLKLSSAEEHFSFLSY
                     KLNEQQHFSSENEIYTSVHLPFIPPEIRDNNFEWTYAEQNKLDQLVELNDIKGIVHTH
                     TQYSDGANTISEMTDYCIRNKFEYLVISDHSKSAFYANGLTEDRLIKQHEEIDKLNTH
                     HPDFKIFKSIECDILNDGSLDYSDEILNTFDLVIASVHANLKMDEGKAMQRLLKAIEN
                     PKMHILGHLTGRLLLSRSGYPVDHKKIIDACAQNGVCIEINANPYRLDIDYKWLQYCM
                     EKNVWISINPDAHNLQGIHDIAYGIHAARKGGLLKTYLINALPAKDFIATLKQFKK"
BASE COUNT         1079 a          572 c          509 g         1027 t
ORIGIN      
        1 ttagaaggca atcacactga aattgccaat aatattaaag gcgatttaca aagtggtact
       61 gtcagtgaaa aaagcttgtt taactcctcc tattttgcaa aaatcggaat aaacaacact
      121 tacaatagct ggacaaatga ggcttttaac cgtgcaaaaa aaggggtaaa gacaagttca
      181 aaactactta tcgggacggt aattacgcaa aataattcag acgtttcatc cgctaaatac
      241 acatctactt ctaagccaaa aaagaagaag tgtataaaaa agaaaggaaa aaaatagtga
      301 tttaagaaat gttgttttta cgcaataaat ctaaaatgac aaatagtgaa aacaacacac
      361 tggcaatacc attagttgta aaaaatgcca agtcaatttt ttgcagtcca aagcgaataa
      421 ccaaactgtg ctgataaata agtaaagctg taaatatggc aaatcctatc caatacaacc
      481 aaccgaacgg atagaaaata cctacaatga gtaagcaaag aaagctgcaa aaatgcaagg
      541 tcctcgatat ccattttgct ttatcctgac caaaatatgc cggtatggaa aacaattgat
      601 tttctttatc aaattgttca tcttgcaagg catataaaat atcgaaaccg cttacccaaa
      661 agaaaacggc aaacgagaaa aacaaaggaa tgatatcaaa cttagccgta actgctaaat
      721 atgcaccaat gggcgccaat gccaatccta cgcctaatat taaatgacat aaaaaagtga
      781 atcttttagt aagggaatac cctaaaataa cggcaagcgc tacaaaagac aaataaaaac
      841 aaagtggatt gataaaataa gtggcaacaa taaaaatcaa gcaattgatg atgacaaatc
      901 ctaatgcatt tttaggtgta ataatacctg atggtatttc acgaatggca gtacgcggat
      961 ttctttcatc atatttcctg tcgatatagc gattaaaagc cattgccgca ttgcgtgcag
     1021 tgaccataca tatcaaaact aaaatcaact tataaagcat aataaaagcc aatggatgat
     1081 tttgataaac atgtttcagt aatgcaggat gaaatgaatc aataaggctg ttgttattgg
     1141 cactttctct caacttggta atacccaaag caaaacctat catcgcaaaa ggcaatgcaa
     1201 acagggtatg cgagaatttt actaaggaca gataattttt cattccttat ttctctttca
     1261 ccgttacact aaaagtaatg gaagttacag gcggattagt atttgcctgg atattgacac
     1321 ttttcgtatt atgacctggt ttatgttttg aatcgaattg aatcttcact ttgccttttt
     1381 tacctggctg taccggttct ttgtcataag ttggaactgt acaaccgcaa gaaccaaatg
     1441 cattttctat aacgagtggt ttcttcccgg tattggtaaa ttctacttct tttgtaatga
     1501 catctccttc cgtacactca ccaaaatcat acacttcttt atcaaatttt atagatgttg
     1561 catttgctaa acgctgttca tcatcagctt cagtaacacg agccatagag tcagcccttt
     1621 ttatcgtagg atcgttttgt acaatagaat catgattaaa atgctgaact tccacattgc
     1681 cacttgtatc tttctgctta cattgaaaaa acatcaatgc tataaaagct gaaataatca
     1741 atccgtaacc tgctattttc atgtatttca tttagagtgc gaatatacaa ttttgccatt
     1801 aacaataatt caatacaaat ttggaaaagc agaaatttaa ctacttttta aattgtttca
     1861 aggtggcaat aaaatctttt gcaggaagcg cattaatgag ataggttttg agcaacccgc
     1921 ctttgcgggc cgcatgaatg ccataagcaa tatcatgtat cccttgcaaa ttatgcgcat
     1981 ccggattgat ggaaatccat acattttttt ccatacaata ttgcagccac ttgtaatcaa
     2041 tatccagacg gtaaggattg gcatttattt caatacaaac accgttttgt gcacatgcat
     2101 caattatttt tttatggtcg acaggataac cgcttctgct taataaaagc ctgcccgtaa
     2161 gatgtccaag tatatgcatt tttggatttt cgatggcttt caataagcgt tgcatagcct
     2221 taccttcatc catcttcaaa ttggcatgta cggatgcaat aactaaatca aacgtattca
     2281 aaatctcatc tgagtaatct aatgatccat catttaaaat atcgcattct atacttttga
     2341 agattttaaa gtctggatga tgtgtattaa gtttatcaat ttcctcatgt tgttttatca
     2401 gtctgtcttc tgttaaacca ttggcataaa atgcactttt gctatggtct gaaataacta
     2461 aatactcaaa cttattccgt atgcaataat cagtcatttc acttatcgta ttagctccgt
     2521 cggagtattg agtatgggtg tgaactattc cttttatatc attcagttcg actaattggt
     2581 ctaatttatt ttgttctgca taagtccatt caaaattatt atcacgaatt tccggtggaa
     2641 taaaaggaag atgaacggat gtatatattt cattttcaga tgaaaaatgt tgttgttcgt
     2701 ttaatttata agataagaaa ctgaaatgtt cttcggctga agacaatttt aataattcaa
     2761 gataaaaaaa ttgtggagtg ctaaaatgaa atgctaccgg ataatcttta tatttttctt
     2821 ggagttgcgt ttgtatttca gcgtcagtta tatccactaa aaaatctacc tgttctaaaa
     2881 taatgttttt tcttctgtaa tctccgcaaa aagaaatttg ttgctgagga aattcttctt
     2941 gtaaagcatc aactatttca ttggctataa gctccagctg tgcccatctg aatttattgg
     3001 cattttgctg aataaaatga atttgctgca agatattttc ttgtgttttt ttgccaaatc
     3061 ctttcaattc cagtaatctg tttttattgc aagcatactc cagttcacca atattttcaa
     3121 tatccagttc cttccaaagc tgtctgattt ttttgggacc tatccctttt atttgcaaca
     3181 tttctac
//
LOCUS       DAOQII010000277         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_19975_length_3184_cov_6.281240, whole genome shotgun sequence.
ACCESSION   DAOQII010000277 DAOQII010000000
VERSION     DAOQII010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19975_length_3184_cov_6.281240"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLF48_10855"
     CDS             complement(<1..493)
                     /locus_tag="PLF48_10855"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNF49872.1"
                     /translation="MNYNTDRSHILMREYGRHLQGMVDFAVKIEDKNERQHAAEAIIQ
                     MMGILNPQLRNSTDYKQKLWDHIFIISDFKLDVDSPYPKPTPENAFIKPSTLPYPQKR
                     IRMKHYGRNVESMVKKAMEMEEDKKIAFTEVIGNFMKMAYKNWSNEEVSNDLVKGDMK
                     TLSA"
     gene            804..>3184
                     /locus_tag="PLF48_10860"
     CDS             804..>3184
                     /locus_tag="PLF48_10860"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNF49873.1"
                     /translation="MQKKFLKLPLLFALLCMAFGMKAQFLGIRASNFGGVTNVNYNPA
                     IADSRYLVDVNLFTTDFTASNSYLGVKGKLLDFKNGWNSDLNLSEKLNGKKKFGYVST
                     TTQLPLSFLVAFGKNKSNNQAIAVTGHLNSVTNVDNLGEALARNIRYGWGTDAFTKIG
                     NFTNQPQSLANIGFKGMAWADLGVTYSRVVLDKEKHFLKVGATLKIVKGLAAAYAYSD
                     KLNYTFDNLDTIHITNTNVYAGATKNLEYFLPSNSGNYDQNSFFTKDILSNKISNWSA
                     AGDVAVVYEFRKDKDKYKYSMDCKDMYYNDRDKHFIQVGLSVVNIGRLKFTKSDSVRD
                     YYLANIKESNPFDQNTLHYTWLNAGTPLNNNSTFKMWLPTTINVWADVNIIKGFGVNV
                     AAQINPIPQKENNVHHLSMVSVTPRYDYKWAGVYMPVAVDQLANVQWGLGFRAGPLYL
                     ASQDILSSLIKLKKGNTRDINIQLGLKVSIPNRTAKDKDKDGVSNKKDLCPMQKGTCE
                     SKGCPDRDGDGILDTEDKCPDVAGLKELQGCPDRDGDGVIDMEDDCPDQAGLKELKGC
                     PDRDGDGIADKDDRCPDVAGLKELQGCPDRDKDGVIDSEDACPDVFGDKAHAGCPDTD
                     KDGLYDNEDKCITVPGPRENMGCPYPDTDGDGVLDKDDKCPTVKGPKDNQGCPVLEEK
                     IKKVLLKARNIQFETGKSVIKPVSYSILNEVAKILKEYDYYNVSIEGHTDNVGNADKN
                     LKLSQDRAKAAMDYLIKQGVSADRMTSSGYGLTKPIADNKTAAGRAQNRRVEF"
BASE COUNT         1060 a          546 c          629 g          949 t
ORIGIN      
        1 ccgccgatag tgttttcata tcgcctttta ccaaatcatt gcttacttct tcgttgctcc
       61 agtttttata agccattttc ataaaattgc ctattacttc agtaaacgca atttttttgt
      121 cctcttccat ttccatggct tttttcacca tagattctac atttcttccg taatgcttca
      181 ttctaattct tttctgtgga taaggcagcg tggagggttt tataaaagca ttttcaggag
      241 taggtttggg atatggcgag tctacatcaa gtttaaagtc tgatatgata aaaatatgat
      301 cccataattt ctgtttatag tcggtagaat ttcttaattg cggattcagt attcccatca
      361 tctggataat agcttcggct gcatgttgcc tttcgttctt gtcttcaatc tttacggcaa
      421 aatccaccat gccttgcaaa tgtctgccat actcacgcat caaaatatga cttctgtcgg
      481 tattataatt catgctaaat aattctgatt tactaggtaa tatataaata agccacacgg
      541 cttatcaata agaagtagga caaaaataca tttttctgcg aataaaacaa tatcagcggc
      601 tttttacctc tgttttgctc attttcctaa acaaaacggg cagatttact tgattgcact
      661 aaacacttaa aatatgttaa taatatgata caattcctta atacaaatgt aactgcacag
      721 aataaagata cagggacata ttcattataa ttttgcactt tcaaaagaga cttactttaa
      781 tattattctt taaaatcaat tttatgcaaa aaaaattttt aaaactgccc ttgctgtttg
      841 ccttgttatg tatggcattc ggcatgaaag cccaatttct tggcattcgg gcaagtaatt
      901 tcggcggtgt taccaatgta aattacaatc ctgcaattgc agacagccgc tatctggttg
      961 atgtgaactt attcaccaca gactttacag caagcaacag ctacttgggt gtaaaaggca
     1021 aattactcga ttttaaaaat ggctggaaca gtgatcttaa tttatctgaa aaactgaatg
     1081 gcaaaaagaa attcggctat gtaagcacaa caactcagct tccactatct ttcttagtag
     1141 cttttggaaa aaacaaaagc aataaccagg cgattgcagt tacaggacat ttaaacagtg
     1201 taacaaatgt tgacaatttg ggtgaggctt tagcccgaaa tatccgttat ggctggggta
     1261 cagatgcatt cactaaaatt ggaaacttta ccaatcaacc acagtcttta gccaatatcg
     1321 gctttaaagg tatggcatgg gctgacttgg gcgttactta ttctagagtg gtattggaca
     1381 aagaaaaaca cttcttaaaa gtaggtgcta ctttgaaaat cgtaaaaggt ttggctgcgg
     1441 cttatgctta ttctgataag ttgaattaca cgtttgacaa tttagatact atccatatta
     1501 ctaataccaa tgtatatgca ggtgctacaa aaaacttaga gtatttcttg ccttctaaca
     1561 gtggtaacta tgaccaaaat agtttcttca caaaggatat tttgtctaac aaaatctcga
     1621 actggtcagc agcaggcgat gttgctgtag tttatgaatt tagaaaagac aaagacaagt
     1681 ataaatacag catggattgc aaagacatgt attacaacga cagagataaa cactttatac
     1741 aagttgggtt atctgtggta aacattggtc gtcttaaatt tacaaaatca gattctgtaa
     1801 gagattacta tttagccaac ataaaagaaa gcaatccgtt tgaccaaaat acattgcact
     1861 atacttggtt aaatgcaggt acgccgctta ataacaacag tacttttaaa atgtggttac
     1921 ctactactat taatgtatgg gctgatgtta atattatcaa aggatttggc gttaatgtgg
     1981 cggcacaaat caacccaatt cctcaaaaag aaaacaatgt acaccatttg agtatggttt
     2041 ccgttactcc tcgttatgat tacaaatggg caggtgttta tatgccggta gctgtagatc
     2101 agttggcgaa tgtacaatgg ggtttaggat ttagagcagg tccgttgtat ttggcatctc
     2161 aagacattct ttcttcttta ataaaactga aaaaaggaaa tacccgtgat atcaacattc
     2221 aattaggatt aaaagtttct atacctaaca gaacagcaaa agataaagac aaagatggcg
     2281 tatcgaataa aaaagattta tgtcctatgc aaaaaggaac atgtgagtct aaaggttgtc
     2341 cggatagaga tggcgatggc atcttagata ctgaagataa atgtccggat gtagcaggtt
     2401 tgaaagaatt acaaggctgc ccggacagag atggcgatgg tgtcattgat atggaagatg
     2461 attgtccgga tcaggcaggt ttgaaagaat tgaaaggttg tccggataga gatggtgacg
     2521 gaattgcaga taaagacgac agatgtccgg atgtggcagg cttaaaagaa ctacaaggtt
     2581 gccctgaccg tgataaagac ggtgtaattg acagcgaaga tgcttgtccg gatgtatttg
     2641 gcgataaagc tcatgcaggt tgccccgata cagataaaga tggtttgtat gacaacgaag
     2701 ataaatgtat taccgttcca ggaccaagag aaaacatggg ttgcccatat cctgatactg
     2761 acggtgacgg cgtattggat aaagacgata aatgccctac agtaaaagga cctaaagaca
     2821 accaaggttg tccggtattg gaagagaaaa tcaaaaaagt attgttgaaa gcacgcaata
     2881 ttcaatttga aacaggaaaa tctgttatca agccggtatc ttattcaata ttgaatgaag
     2941 tagctaaaat tttgaaagaa tatgactact ataatgtaag tatagaagga cacactgata
     3001 atgtgggtaa tgcagacaaa aacttgaaat tgtcccaaga cagagcaaaa gcagctatgg
     3061 actacttgat taagcaaggt gtttctgcag acagaatgac ttcatcagga tatggcttga
     3121 caaaaccgat tgcagacaat aaaactgcag ccggtagagc gcaaaaccgt cgtgttgagt
     3181 tcaa
//
LOCUS       DAOQII010000278         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_20002_length_3182_cov_3.742565, whole genome shotgun sequence.
ACCESSION   DAOQII010000278 DAOQII010000000
VERSION     DAOQII010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20002_length_3182_cov_3.742565"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PLF48_10865"
     CDS             <1..932
                     /locus_tag="PLF48_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HNF49874.1"
                     /translation="MQAICKEVGIKATVWNQKKIEQEKMGGILAVNKGSLEPGTFNIL
                     EYKPAKPKNSKPLVLVGKGVVFDTGGISLKPGAGMEAMKCDMAGSACVLGGMYAIAKA
                     KLNYHVIGLIPAVENRPSDRAICPGDIITMYDGSTVEVLNTDAEGRLILADALQYAKK
                     LNPELVLDFATLTGSAMRAIGKEAAVIMGTADKAMKKKIRKAANKTGDRVVEFPMWRE
                     YGEYMKSDIADLKNLGPAEAGSITAGKFLEYFTSYPWMHFDIAGPAFVTSPYSYRGKW
                     ATGYGVRLLFTFVKNLCKDDSEKRETESRHNAG"
     gene            886..1953
                     /gene="pdxA"
                     /locus_tag="PLF48_10870"
     CDS             886..1953
                     /gene="pdxA"
                     /locus_tag="PLF48_10870"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNF49875.1"
                     /translation="MIVKKEKLRVGITLGDVSGIGPEIIMKTFADEQIYKHFTPILFG
                     NPRVLSYYKKAIDGVEKFQYTTVKGYGNLSNNSLNIIPVWEEEFQINIGEPTETTGKY
                     AFKSLAAACSALKDGNIDVLITAPINKKYIHSSEFAFAGHTDYLKQYFGANEVLMLMS
                     SDKIKLGLVTDHVPIKEVASKISKDKILRKLHILNATLMEDFGIDKPRIAVLGLNPHA
                     GDGGLIGTEDRDIILPAVNDAKNDGMVVFGPYPADGFFASGAYTKFDGILAMYHDQGL
                     IPFKYIAGEEGINYTAGLKVIRTSPDHGTAEDIAGKSIADESSFRQAIYSAIDIYRNR
                     ENFYEMRSNPLVKRAKFQEER"
     gene            complement(1962..>3182)
                     /locus_tag="PLF48_10875"
     CDS             complement(1962..>3182)
                     /locus_tag="PLF48_10875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNF49876.1"
                     /translation="DERRNMLTKDETMRIEPLLNTEILKGGGMYVEYRSDDARLTIEV
                     FKTAVARGAICVNYVACKSLQYDENNKINGVLAHDVITNTEFTIKTKKIINACGPWVD
                     EIREKDNSLKKKRLHLTKGIHIVVKKERIPLYQSTYFDVIDGRMIFAIPRGNKVYIGT
                     TDTNYKQDKNYPFADIEDVTYLLNAVNHMFPSVNLSVTDIESSWAGLRPLIHEDGKSP
                     SDLSRKDEIFISDSNLISIAGGKLTGFRKMAERSVNVVCKQLSKEENRIFQPCTTADI
                     QLTGGDLQTEPQEYARELQLLYKQFYLDDVTDLVLKYGSNTSYILAAAHEEFQNHLLQ
                     AEMEYCMENELCTNLSDFIIRRTGRLYFDRPQTANLIPVIENYFNTELNIPEEQAAKN
                     KAAFMKEFEGVKRF"
BASE COUNT          959 a          586 c          706 g          931 t
ORIGIN      
        1 atatgcaggc aatttgtaaa gaagtgggaa ttaaagcaac agtctggaat caaaaaaaga
       61 tagaacagga aaaaatgggc ggtattctgg ccgtaaacaa gggcagttta gagcctggca
      121 cttttaatat actggaatat aaaccggcta aacccaaaaa cagcaaaccg ctggtactag
      181 ttggaaaagg cgtggtgttt gatacaggtg gcatttcgct aaaacccggt gccggcatgg
      241 aggcgatgaa atgtgatatg gcaggttcgg cttgtgttct tggtggaatg tatgcaatag
      301 ctaaagcaaa attaaattat catgttatcg gcttgatacc agcggttgaa aaccggccat
      361 ccgacagagc catttgtccg ggagatatca ttacgatgta cgatggaagt accgtagaag
      421 tgctgaacac ggatgcagaa ggtcgtttga ttttggcaga tgcattacaa tatgcaaaaa
      481 aacttaatcc ggaattggtt ctggattttg caacattaac gggttccgcc atgcgggcaa
      541 taggtaaaga agcagctgtt attatgggaa ctgcggataa agcgatgaaa aagaaaattc
      601 gcaaggcagc aaataaaacc ggtgacagag tggtagagtt tccaatgtgg cgagagtatg
      661 gcgagtatat gaaaagtgat attgccgact tgaaaaattt aggtccggcc gaggcgggtt
      721 caattactgc aggaaaattt ctggaatact ttacttcata tccttggatg cattttgata
      781 tagcaggtcc tgccttcgta acttcacctt atagttacag aggaaaatgg gctaccggct
      841 atggtgtcag attattgttt acctttgtga aaaatttgtg taaggatgat agtgaaaaaa
      901 gagaaactga gagtaggcat aacgctgggt gatgttagcg gaattggtcc cgaaataatt
      961 atgaaaactt tcgcggacga acagatttat aaacatttta ctcctatttt attcggtaac
     1021 cccagagtgt tgtcgtatta taaaaaagcc attgatggtg tcgaaaaatt tcagtacact
     1081 accgttaagg gttatggcaa tctgagtaat aattctttga atattattcc ggtttgggaa
     1141 gaagaatttc aaatcaatat aggtgagcct actgaaacta ccggaaaata tgcattcaaa
     1201 tccttagctg ctgcttgttc ggctcttaaa gacgggaata tcgacgtgct gatcacagca
     1261 ccaataaaca aaaaatatat ccactccagt gagtttgcct ttgccggaca tacggattat
     1321 ctcaaacaat attttggtgc gaatgaagtg ctgatgttga tgtcgtcaga caagataaaa
     1381 cttggtttag ttaccgatca cgttccaatc aaagaggttg catcaaaaat ttcaaaagat
     1441 aaaatactgc gcaagctgca tattctgaat gccacactga tggaagattt tggtattgac
     1501 aaaccgcgca tcgccgtgtt gggattaaac ccgcatgcag gagatggcgg acttattggc
     1561 acagaagacc gggatattat tttacccgca gtaaatgatg caaaaaatga tggcatggtc
     1621 gtatttggtc cttatccggc tgacggattt tttgcatcgg gtgcttatac aaaatttgat
     1681 ggcatcttag ccatgtatca cgatcagggt ttgattcctt ttaaatatat agcaggtgag
     1741 gagggcatca actatacggc cggattaaaa gtcatccgca cttcgcccga ccatggaaca
     1801 gccgaagata ttgccggcaa aagcattgct gatgaaagtt cattccgtca ggcaatatat
     1861 tctgcaatcg atatctacag aaacagagaa aatttttacg aaatgcgcag taatccgttg
     1921 gtaaaacgag cgaaattcca ggaagaaaga taggctttag tttaaaagcg tttaacgcct
     1981 tcaaattctt tcataaatgc agctttgttt tttgcagctt gttcttcagg gatatttaat
     2041 tcggtattaa aataattttc gataacagga atcagattag cagtctgagg acggtcaaaa
     2101 tacaatcttc ctgtacggcg gataatgaag tcggaaagat tggtacataa ttcattttcc
     2161 atgcaatatt ccatttctgc ctgtaataaa tgattttgga attcttcatg agctgctgct
     2221 aaaatatagg aagtgttcga gccatattta agcacaaggt cagtaacatc atccagataa
     2281 aattgtttgt aaagcaactg tagctcgcgg gcatattctt gaggttctgt ttgcaaatca
     2341 ccaccggtca gttggatatc tgccgtagta caaggctgga atatgcggtt ttcttctttg
     2401 ctcaattgct tgcaaacgac attcacactg cgttcggcca tttttctgaa tccggtaagc
     2461 ttgccgccgg ctattgaaat caaattggaa tcagagataa aaatttcatc ttttcttgat
     2521 aaatcggatg gcgattttcc gtcttcatgt atcagtggtc gcaagcccgc ccagctgctt
     2581 tcaatatccg taacactcaa attgacagat gggaacatgt gattgactgc attcaacaag
     2641 taagtaacgt cttcaatatc agcaaaagga tagtttttat cttgtttgta attcgtatct
     2701 gttgtgccga tatatacttt atttcctcgt gggatagcaa agatcattct gccgtcaatg
     2761 acatcaaaat aggtggattg atagagcggt atgcgttcct ttttcactac gatatgaata
     2821 cccttagtaa gatgaagtct tttctttttt aatgaattgt ctttctcccg tatttcatcc
     2881 acccaagggc cgcaggcatt gattatcttc ttcgttttga tagtaaactc agtattggta
     2941 atgacgtcgt gagcaagaac accatttatc ttattatttt cgtcgtactg caaactctta
     3001 caggcaacat aattcacgca aattgccccg cgggctacgg cggttttaaa cacttcaatg
     3061 gtcaggcgag catcatcact tcgatattcg acatacatac cgccgccttt taaaatttcg
     3121 gtattcagta aaggttctat gcgcatagtt tcgtctttgg tcagcatatt gcggcgttca
     3181 tc
//
LOCUS       DAOQII010000279         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_20114_length_3172_cov_5.304139, whole genome shotgun sequence.
ACCESSION   DAOQII010000279 DAOQII010000000
VERSION     DAOQII010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20114_length_3172_cov_5.304139"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLF48_10880"
     CDS             complement(<1..881)
                     /locus_tag="PLF48_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49877.1"
                     /translation="MRKYNSSRLHISGFSYCIILLIFSCSFLQIQAQTATAVVKEKEY
                     LIGDWIPVDLYVEAKSNARVVFPNLKDSISEIIEVANFTPVDTVRKSGVNQYHQLVNL
                     IVFDTGRILLPQFQFLIDNSGKIDSITTSAEMIHVAGVEIDTTQDIKPIKEPLKIPLT
                     FKEVLPFLIGAFVLAVLGALLAWYFIEKRKKLKPVDEKYLLPPHVWAVQQLQKLNQKK
                     LWQTGNVKEYYSELTDIARTYIELRYKIPAMEQTTDELMDAMHKGVLKQSLKKELNEV
                     LTLSDLVKFAKAQPDML"
     gene            complement(981..1847)
                     /locus_tag="PLF48_10885"
     CDS             complement(981..1847)
                     /locus_tag="PLF48_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNF49878.1"
                     /translation="MEVNELLKKVRQIEIKTRGLTNHIFSGEYHSAFKGRGMSFSEVR
                     EYQYGDDVRNIDWNVTARFKQPYIKIFEEERELTVMLLIDVSKSVFIGTQQQPKNEII
                     SEIAGILSFSAIKNNDKVGVLFFSDKVEMYIPPKKGKSHILRIIREILNFTPSSHNTN
                     ISLALASVYNMVKRKSIVFIISDFMDENYADELKHVAKKHDTIGIKVFDALDNELPKI
                     GLLQVQDVETGQTHWIDTNDKTTLSAYKERSVFYDKNFENSFIKSGADKIKINAAKPY
                     VHLLMNFFKKRA"
     gene            complement(1930..2922)
                     /locus_tag="PLF48_10890"
     CDS             complement(1930..2922)
                     /locus_tag="PLF48_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNF49879.1"
                     /translation="MESVDIKALNEKIQQDSAFLDLLQLEISKSIVGQKYMLERLMIA
                     LLSQGHILLEGVPGLAKTLAIKSLSEAINSNFSRIQFTPDLLPADILGTMIYNQAKGN
                     FEVKKGPIFSNFILADEINRAPAKVQSALLQAMQERNVTLGDTTYKLEEPFLVMATMN
                     PIEQEGTYNLPEAQVDRFMLKVVIGYPEFEEERQIIRQNVSGVNPGIKPVVSPHEILK
                     ARDTVRTVYMDEKIEKYILDIVFATRDPQKHKIDNLKHLIAYGGSPRASISLALASKS
                     YAFIKRRGYVIPEDVRAVAHDVLRHRIGVTYEAEAENITQENIIDTILNRVEVP"
BASE COUNT          905 a          623 c          548 g         1096 t
ORIGIN      
        1 tccagcatat ccggttgggc ttttgcaaat tttaccaaat cacttaaggt cagtacttcg
       61 ttcagttctt ttttcagcga ttgttttaat acgcctttgt gcatggcatc cataagctca
      121 tccgtggttt gctccattgc aggaattttg tagcgcagtt caatatatgt tctggcaata
      181 tccgtcagct cagaataata ttcttttaca ttcccggttt gccacagttt tttctgattt
      241 agtttttgca gttgttgtac tgcccagaca tggggaggca acaagtattt ttcatctact
      301 ggttttagtt tttttctttt ttctataaaa taccatgcca gcaatgcccc taatactgct
      361 aagacaaatg ctcctatcaa gaatggaagc acctccttaa aagtaagtgg aatttttagc
      421 ggttctttta tgggttttat atcctgtgtt gtatcaattt caacaccggc tacatgaatc
      481 atttcagcag atgttgttat agaatctatt tttccgctat tatctatcaa aaactgaaac
      541 tgtggcaaaa gtatcctgcc ggtatcgaat acaattaaat ttactaactg atgatattgg
      601 ttaacgcctg attttcttac cgtgtcaacc ggagtaaagt tggctacttc tattatctca
      661 gagatagaat cctttagatt cggaaataca acgcgcgcat tactttttgc ttcaacatat
      721 aaatctaccg gaatccagtc tcctatcaga tattcttttt ctttaactac agccgtggct
      781 gtctgcgcct gtatttgtaa aaaggagcaa ctgaaaatca gtagaatgat acaatatgaa
      841 aatccggaaa tgtgaagacg tgaagaatta tatttcctca tgctacacaa caaattttca
      901 atatgccacc aaattaatct caaaataatt tcgtttactt tttttagctc tattattaat
      961 tttctaatta tctcattatg ctatgctctt ttcttaaaga aattcatcaa tagatgtaca
     1021 taaggctttg cagcatttat ttttatcttg tcagctccag acttgataaa actgttttca
     1081 aaatttttgt cataaaaaac actccgctcc ttatatgctg acaaggtagt tttatcgttt
     1141 gtatcaatcc aatgtgtttg tccggtttct acatcttgta cttgcagcaa gcctattttt
     1201 ggcaattcat tatccagtgc atcaaacact tttatgccaa tggtatcgtg ttttttagca
     1261 acatgcttaa gttcgtcagc ataattttca tccataaaat cggaaatgat aaaaactata
     1321 cttttgcgtt tcaccatatt gtatacactt gctaaagcca gtgagatatt ggtattatgt
     1381 gaggaaggcg taaaattcag tatctctctg atgatacgca gtatatgtga ttttcctttc
     1441 tttggaggga tatacatttc caccttatca ctaaaaaaca agacgcccac tttatcatta
     1501 tttttgatag ctgaaaaaga taagataccg gctatttcag aaataatttc attcttaggt
     1561 tgctgttgtg tgccgataaa aaccgatttg cttacatcta tcagcaacat aactgtcagc
     1621 tctctttctt cttcaaatat tttgatataa ggttgcttaa accttgcggt tacattccag
     1681 tcaatattac gcacatcatc accatactga tactctcgca cttcgctaaa cgacataccc
     1741 cgtcctttga acgcagaatg atactcaccc gaaaaaatat gattcgtcaa gcctcgagtc
     1801 ttgatttcaa tctgacgtac ttttttgagc aattcgttta cttccatatg gaaacgataa
     1861 atgttggatt ttagatttta aatggaataa tcattacaac ttagctaatt attcagattg
     1921 ccaaatcggc taaggcactt ccactctgtt cagtattgta tctatgatgt tttcctgtgt
     1981 gatattttca gcttctgctt cgtaagtgac tccaatgcgg tgacgcaaca catcatgtgc
     2041 tacagctctc acatcctctg ggattacata acctctgcgt ttaataaatg catacgattt
     2101 ggaagccagt gccagactga tacttgcacg aggcgaacca ccatatgcaa taagatgttt
     2161 cagattgtct attttatgtt tctgagggtc gcgggttgca aacacgatat ccaaaatgta
     2221 tttctctatc ttttcgtcca tgtaaacggt acgaactgta tctcgtgctt ttaatatttc
     2281 atgtggcgac acgacgggtt taattcccgg attgacacca cttacatttt ggcggatgat
     2341 ttgccgttct tcttcaaatt caggataacc tattaccact ttcaacatga agcggtcaac
     2401 ttgcgcttcc ggcaaattat aagttccttc ttgctcgatg gggttcatgg tggccatcac
     2461 taaaaaaggc tcttccagtt tataggtcgt gtcgccaagc gttacatttc tctcctgcat
     2521 agcttgcagc aaggcactct gtacttttgc gggtgcacgg ttaatttcgt ctgccaagat
     2581 gaaattggaa aatataggtc ctttttttac ttcaaaattg ccttttgcct ggttataaat
     2641 catcgttccc aaaatatcgg caggcaataa atcgggtgta aactgaatac gggaaaaatt
     2701 tgaattgatt gcctcggaca aagattttat ggcaagcgtt tttgccaaac cgggtacacc
     2761 ttccaacaaa atatgtcctt gcgataagag tgcaatcatt agtcgctcga gcatgtattt
     2821 ttgcccgacg atagattttg aaatttccag ttgcaacaaa tcgagaaaag cgctgtcctg
     2881 ttggattttc tcattgagag ctttaatatc tacagattcc atgtataaat ttttgaatac
     2941 gcaattgatt tagaaaggca caaaaataaa ggctagaatg aatacttcat tttttgtcgt
     3001 gttaaaaaat gttagatgat ttaacattta ggaaattttt aatcagctgg aagatattaa
     3061 gcaataatgg ttggtatact taccagtcca tcctcatttg ttcagtcagg acaccaaacg
     3121 ataagagaag ccgttatagt gtacatttta ttttcgagca tggtataatg tg
//
LOCUS       DAOQII010000280         3152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_20344_length_3152_cov_4.197288, whole genome shotgun sequence.
ACCESSION   DAOQII010000280 DAOQII010000000
VERSION     DAOQII010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20344_length_3152_cov_4.197288"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1120)
                     /gene="holA"
                     /locus_tag="PLF48_10895"
     CDS             complement(101..1120)
                     /gene="holA"
                     /locus_tag="PLF48_10895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNF49880.1"
                     /translation="MAVTLETIWKDLHAKKFKPVYFLAGEEAYYIDLIENFIEKNALQ
                     DHERDFNQAIFYGKDADPIQVLNEAKQYPSFAERRLVIVREAQEFKAKDWELIEKYIQ
                     KPVESTVLIFSHKNKLPDKRTNFYKSLSKHTEYFESNKVSEDKLADWIAGYVKNKRNY
                     KIQLSTAQVLAENLGNDLSRIANEMDKLSVMLPDNAEITPEIIEKYIGISKEYNMTEF
                     NRAVAQRDFHKAMKMVMYFEKNPKAGPIVAILAYMYNLFARLLIMLNAKDTSSASLQM
                     GFGGKWIPDEYKFASKVYSKAETQKILSYLCEYDAKAKGLYATDNATSTDLLKELLVK
                     IVSRK"
     gene            1137..1565
                     /locus_tag="PLF48_10900"
     CDS             1137..1565
                     /locus_tag="PLF48_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNF49881.1"
                     /translation="MQNTAFRLKKEKDKTFIFDIVRKKWIVFTPEEQVRQYCIRYLTD
                     ILLVPLSFISVEKSLTINGLKKRYDIVVYNKNLKPVLLVECKADTEALDEKVCKQIAI
                     YNLQLNVPYLWLSNGKKNYYFNLEENKILQIPALPFYDEM"
     gene            complement(1648..2901)
                     /locus_tag="PLF48_10905"
     CDS             complement(1648..2901)
                     /locus_tag="PLF48_10905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNF49882.1"
                     /translation="MQEKTIKIHAPLFTALEESLHAIFEQGVYADKEVARALKANRNW
                     GSRDRSFFAETVYDIVRWKRKYAFQLEQSAIQDPKNNVQALIGISLLNRNYAIQNPER
                     TRLTSADIDLLKKQLLMPIAQPAIATSYPVELYNYCEQALGNTWHAIAEQLNTQAKVY
                     LRVNTLKSTKKKISEILEKENIAFRDDCQTVFSPYLLEDSLEIITKNKLKNSTHYTNG
                     YFEFQDIGSQLISVFALQNIPDFSNFTLLDMCAGAGGKTLHLSTLMKNKGKIIATDFN
                     EKRLVQLQKRIQHAGCKNVEITDFSALQNMTNRFDFILIDAPCSGLGTLKRSPDIKWK
                     LSIRDIENYCNIQQKLLHDASTLLNKNGTMLYATCSILPQENENQIKRFLENQTAFKL
                     AAEIKINPAHYPSDGFYMAKLQIKK"
     gene            complement(2901..>3152)
                     /locus_tag="PLF48_10910"
     CDS             complement(2901..>3152)
                     /locus_tag="PLF48_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF49883.1"
                     /translation="LIHEIDQHGNYKTTHLSLNTQLQYVVVAGNWFAVTAQGNLDFSL
                     CGCTVIPAFSFETFELATCQKLKATFPQHADIIQQLTRS"
BASE COUNT          889 a          571 c          582 g         1110 t
ORIGIN      
        1 atgggtttgg actatagggt aatgagtatg ataaggataa agtataatta ttaaattaat
       61 tctgcctgct tacaacttac tacttattac ataccacttt ctactttctg ctaacaatct
      121 ttaccagcag ttctttcagt aagtctgtgg aggtggcgtt gtctgtcgca tataatcctt
      181 ttgctttagc atcatactca cacaggtaac tcaatatttt ttgggtttcg gctttcgaat
      241 atactttcga ggcaaactta tactcatccg gaatccattt tccgccaaag cccatttgca
      301 acgaagcaga tgaggtatct tttgcattta gcatgatgag caggcgggca aataaattat
      361 acatataagc caaaatagct acgatgggac cggcttttgg atttttttca aaatacatca
      421 ccattttcat cgctttatgg aaatcacgct gtgccaccgc ccgattaaat tccgtcatgt
      481 tgtattcctt agaaatgccg atatattttt caataatttc aggcgtaatt tctgcattat
      541 ccgggagcat caccgagagt ttgtccatct cgttggcaat acgcgataag tcattgccta
      601 agttttctgc cagcacttgc gctgtagaca actggatttt atagttgcgt ttgtttttta
      661 catatcctgc aatccagtca gccagcttgt cttcactcac tttattcgac tcgaaatatt
      721 cggtatgttt agagagtgat ttgtaaaaat tggttcgctt atccggtaat ttgtttttgt
      781 gtgaaaaaat aagcaccgta gattctacag gcttttgaat gtatttttca atgagctccc
      841 agtctttggc tttaaactct tgcgcttcgc gtacaataac taatcttctt tcggcaaaag
      901 atggatattg tttggcttcg ttcagtactt gaatcgggtc ggcatctttg ccataaaata
      961 tcgcctgatt gaaatcgcgt tcatggtcct gaagcgcatt tttttctatg aaattttcaa
     1021 tcaaatcaat gtaataagct tcttcgccgg caagaaaata taccggtttg aattttttag
     1081 catgcaaatc tttccaaatc gtttctaagg taacagccaa tgtcgtattt ttatgcgtgc
     1141 aaaatactgc gtttcgccta aaaaaagaga aagataaaac attcattttc gacatcgttc
     1201 ggaaaaagtg gatagttttc acgcccgaag agcaagtaag gcagtattgc atccgttatt
     1261 tgacggatat actgcttgtg cctctatcgt ttatttctgt tgaaaaaagt ttgaccatca
     1321 acgggctgaa aaaaaggtat gatattgtcg tgtataataa aaacctgaaa cccgtgttgt
     1381 tggttgaatg caaagcggat acagaagccc tcgatgaaaa agtgtgcaag caaattgcca
     1441 tttataatct gcagttaaat gtgccttatt tgtggttgag caacggcaaa aagaattact
     1501 atttcaacct ggaagaaaat aagattttac aaattccagc cttgcccttt tatgatgaaa
     1561 tgtgattgaa gggagttttt aagtaagtag attttttatg aaattaattg acaaaaatct
     1621 ttgaaatgtt tttttctaat ttgcagctta ttttttgatt tgcaacttcg ccatataaaa
     1681 accatcactg ggatagtgtg ccgggtttat ttttatttct gccgcaagtt taaaagcagt
     1741 ttgattttcc agaaatcgtt taatttggtt ttcattttct tgcggcaaaa tactgcatgt
     1801 ggcataaagc atagtcccgt ttttgttgag caatgtgctt gcatcgtgca ataatttttg
     1861 ttgtatgtta cagtaatttt cgatgtcacg aatgcttaat ttccacttga tatccgggct
     1921 tctttttaaa gtgcctaatc cgctacatgg cgcatcaatt aagataaaat caaagcgatt
     1981 ggtcatattc tgtaaagctg aaaaatctgt aatctctaca tttttacatc cggcatgttg
     2041 tattcttttt tgtaattgta ctaatctttt ttcattaaaa tccgtagcga taatctttcc
     2101 tttatttttc atcaatgtcg ataaatgcaa ggttttacca cctgcacctg cacacatatc
     2161 cagtagcgtg aaattgctga aatctgggat gttttgaagg gcaaacacgc tgattaattg
     2221 cgaacctatg tcctgaaact caaaatagcc attggtataa tgagtggagt ttttaagctt
     2281 gttttttgtt ataatttcca gactgtcttc caacagatag ggtgaaaaaa cggtttgaca
     2341 atcatcgcga aatgctatgt tttccttttc taatatttca cttatttttt tctttgtaga
     2401 ttttaaggta ttgactcgca aatagacttt agcttgagta ttaagctgct cagcaattgc
     2461 atgccacgta ttgccaagtg cttgttcgca ataattatat aattcaaccg gataagatgt
     2521 tgcgatagca ggttgcgcga tgggcatcaa aagttgtttt ttcaagaggt caatgtctgc
     2581 tgaagtcagc cttgttcttt ccggattttg aatagcataa tttctgttta ataagctgat
     2641 tccaattaac gcttgtacat tatttttagg atcttgtatc gcagattgct ccagctgaaa
     2701 tgcatatttt cttttccatc taacaatatc ataaactgtt tccgcaaaaa aactcctgtc
     2761 gcgcgaaccc caatttctgt ttgcttttaa tgcacgtgcc acttctttat ctgcataaac
     2821 tccctgttca aaaattgcat gtaaactttc ttcaagagca gtaaacaaag gggcatgtat
     2881 ttttatagtt ttttcctgca tcagcttcgt gtaagttgtt gtataatatc agcatgttgg
     2941 ggaaatgttg cttttaattt ttggcaagtt gccaattcaa aagtttcaaa tgaaaatgcc
     3001 ggaatgacgg tgcatccgca aagtgaaaaa tctaaattgc cttgtgctgt aaccgcaaac
     3061 cagttgcctg ctactacaac atattgcaat tgtgtattca gactcagatg ggttgtttta
     3121 taatttccgt gttggtcaat ttcatgaata ag
//
LOCUS       DAOQII010000281         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_20360_length_3151_cov_4.775194, whole genome shotgun sequence.
ACCESSION   DAOQII010000281 DAOQII010000000
VERSION     DAOQII010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20360_length_3151_cov_4.775194"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLF48_10915"
     CDS             complement(<1..245)
                     /locus_tag="PLF48_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49884.1"
                     /translation="MNTKSLRIKICGLKDASQILQIADMQPDFIGFIFYRNSPRYILD
                     GLNLNEQVNMQFQLSAISHPVQKVGVFVNEDIKSLIR"
     gene            complement(245..1564)
                     /locus_tag="PLF48_10920"
     CDS             complement(245..1564)
                     /locus_tag="PLF48_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49885.1"
                     /translation="MIILDFNFTKIIRVLIFTALFFFCELNSEAENKYLISYTKVNSY
                     SKKEIKSIWKKHHIPKIILPVNEGIDVYEILYKAPWIDSSLITCSGIYYVPQSSKAMP
                     YVMYGHGTQIERSRTISPDDAQQGICLGFAADGYLACYPDYYGIGKGEKRHLYQHAWS
                     EAMSFIYMLYAVDEMNKALAIKTNGQLFLTGYSQGGHSSFAAQKYIEALHDPRFKVTA
                     SSPMSGAYDMTGEQDKFMFQPYPRQFYLPYLLTSYQLAYHILPYTNIYSIFKSPYDTL
                     LPNYFENNDHKTLYELNKMLPPIPKDMVLDSLVNAYQQDNNYIFKQKLRENNLTNWKP
                     EAPVQLCFCKGDREVSYKNSIAVYDTMTTSGVKAIKLNNLSDHLDHNTCAPFAVLATK
                     YFFDRYKKHGTNPKMKDVPKFKLKLVGIVKKKEEKKYLKEKKDNIRY"
     gene            complement(1590..2150)
                     /locus_tag="PLF48_10925"
     CDS             complement(1590..2150)
                     /locus_tag="PLF48_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF49886.1"
                     /translation="MQAHFFASQSEFRKWLENNHQTEKELIVGYYKVNSGKPSMTWSQ
                     SVDQALCFGWIDGIRKSINDESYSIRFTPRKKNSIWSAVNIRKMEELSKSGLMTEFGL
                     QAFSHKKDEKSKMYSYEIGQVELPESYLNIFKKNTDAWIFFEKQPTHYKKNTYKWIMT
                     AQLDKTKLSRLEKAIHHSTQQLRLKW"
     gene            complement(2153..>3151)
                     /locus_tag="PLF48_10930"
     CDS             complement(2153..>3151)
                     /locus_tag="PLF48_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49887.1"
                     /translation="KFGVEHALAVSVFMIILEIYLGIALILGTWKKLTLWLLVLLILF
                     FTFLTGYSAKTNKVTDCGCFGDFIKLAPIQSFYKDILLTVLSFILLFGQKYIKDILGV
                     IPNRIILGVLTLASIWFTYRNIVHEPIVDFRPYKVGTSVPDCLKLPPNAKKTIVDMVY
                     TYKNKKTGEVKEFKNSYPDPADYANWQYLDRKDVIVQKGDEPKCKDFIIKDANSGDNT
                     AMFFEEPEYLFFILSPNLDKANEKAFSKMIPVTEDAIKDGKYAFVITGSIPSEAEAFK
                     AKMKIPYDFYFMDPTPIKTIMRSNPGLLIMKQGKIIAKFHYNDVPSYSDIKKLYLK"
BASE COUNT          938 a          574 c          550 g         1089 t
ORIGIN      
        1 atacgtatca gcgatttaat atcttcattg acaaacacac ccactttttg tacgggatga
       61 ctgatagctg acagttgaaa ttgcatattc acttgctcat tgagattgag tccgtccaaa
      121 atatatctcg gcgaattccg gtaaaatata aagccgataa aatcgggttg catgtctgca
      181 atttgaagta tctgcgatgc atcttttagc ccgcaaattt ttatgcgcaa tgatttggta
      241 ttcattaata acggatatta tctttttttt ctttcaggta ttttttttct tctttctttt
      301 tcacaatgcc caccaattta agtttaaatt tcggcacatc tttcatcttc ggattagtgc
      361 cgtgcttttt atatcggtcg aaaaaatact tagtggccaa cacagcaaat ggtgcacaag
      421 tattatggtc caaatggtcg ctcaggttgt tgagttttat tgccttaacg ccagaggtgg
      481 tcatggtgtc atacacagca atcgaatttt tataactgac ttctctgtcg cccttgcaaa
      541 aacacagttg caccggcgct tccggtttcc agttcgtcaa attattttcc ctcagctttt
      601 gtttgaaaat gtaattatta tcttgttggt aggcattcac taggctatcc agcaccatat
      661 ctttagggat gggcggcagc attttattta gctcatacag cgttttatga tcattgttct
      721 caaagtagtt gggcagtaga gtatcataag gcgatttaaa aatggaataa atattagtgt
      781 aaggtaaaat atgataagca agctgataag aggttaataa ataaggcaaa taaaactggc
      841 ggggatatgg ctgaaacata aatttatctt gttcgcccgt catatcgtaa gcgccgctca
      901 tgggcgaact tgccgtaact ttaaaacgtg ggtcgtgtaa agcctcaata tatttttgtg
      961 cagcaaaaga ggaatgtccg ccttgtgaat agcccgttaa aaatagctgt ccgtttgttt
     1021 taatggcaag tgctttattc atttcatcca cggcatacag catataaata aaagacattg
     1081 cctcactcca ggcatgctga tacaaatgcc gcttctcccc ttttccaatg ccataataat
     1141 cgggataaca agccaaatag ccatcggcag caaaacctaa gcaaatgcct tgttgtgcat
     1201 catccggact gattgtacgg ctgcgttcta tttgcgtgcc atgtccatac atgacataag
     1261 gcatggcttt ggagctttgg ggaacataat aaatacccga acaggtaatc aaggaactgt
     1321 ctatccaggg agctttgtat agtatttcgt acacatctat accttcattt acgggcaaaa
     1381 taatttttgg gatatggtgt ttcttccaga tggattttat ttcttttttt gaataggaat
     1441 ttactttagt ataagaaata aggtatttat tttcggcttc tgaatttaat tcgcaaaaga
     1501 agaatagtgc cgtaaaaatc aaaacacgta tgatttttgt aaaattaaaa tctaaaataa
     1561 tcatattttt atttataatt aatagtaaat taccatttta atctaagttg ttgtgtagaa
     1621 tgatggattg ctttttccaa tctggataat ttcgttttat ctaattgggc agtcattatc
     1681 catttgtacg tgtttttttt ataatgagta ggttgttttt caaaaaaaat ccatgcatcg
     1741 gtattttttt taaaaatgtt caaataagat tctggcaatt ctacctgtcc aatttcataa
     1801 ctatacattt ttgatttctc atctttttta tgactgaaag cttgcaatcc aaattcagtc
     1861 atcagtccgg attttgacaa ctcctccatt ttacgaatgt taacagcact ccaaatactg
     1921 ttttttttgc gtggggtaaa acgaatagaa taactttcat cattaattga tttccggata
     1981 ccgtctatcc atccaaaaca tagtgcttga tctacagatt gcgaccatgt catagaaggc
     2041 ttgcccgaat ttactttgta atatcctact attagctctt tttctgtttg gtgattattt
     2101 tctaaccact tcctaaactc actttgtgaa gcaaaaaaat gagcttgcat atttatttaa
     2161 ggtataattt ttttatatcg ctataactcg gcacatcgtt ataatgaaac ttagcgatta
     2221 ttttgccttg tttcataatc aataagcccg gattggagcg catgatggtt ttgataggtg
     2281 tcgggtccat aaaataaaaa tcatacggaa ttttcatttt tgctttaaaa gcttctgctt
     2341 ccgatggtat gctgcctgta atcacaaatg catatttgcc atctttaatc gcatcttctg
     2401 ttaccggaat cattttgctg aatgctttct cattggcttt atccaaatta ggacttaaaa
     2461 taaaaaacag atattcgggt tcttcaaaaa acatggcagt attgtcacca ctgtttgcat
     2521 cttttataat aaagtcttta cattttggct catctccttt ttgcactatc acatcttttc
     2581 tgtccaggta ttgccagttt gcataatctg ccgggtcggg atatgaattt ttaaactctt
     2641 tcacttcacc cgttttcttg tttttataag tatacaccat atctactatg gttttctttg
     2701 catttggtgg caatttcaga cagtctggta cagatgtacc tactttatac ggtcggaaat
     2761 ctactatcgg ttcgtgtacg atattgcggt aagtaaacca aatggatgcc aaggttaata
     2821 cacctaaaat aatacgattt ggaataacgc ctaaaatatc tttaatatat ttttgaccaa
     2881 acaacaagat aaaactcaat acggtgagta aaatgtcttt gtaaaaagat tgtatcggag
     2941 ccagttttat aaaatcgccg aaacagccgc aatcggttac tttgttggtt ttggcagaat
     3001 atccggtcag gaaagtgaaa aataaaatca gcaataccaa caaccaaagt gtcaattttt
     3061 tccaagttcc taaaatgagg gcaataccca aataaatttc gaggataatc ataaatacag
     3121 atacagcaag tgcatgttca actccgaatt t
//
LOCUS       DAOQII010000282         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_20392_length_3148_cov_4.692855, whole genome shotgun sequence.
ACCESSION   DAOQII010000282 DAOQII010000000
VERSION     DAOQII010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20392_length_3148_cov_4.692855"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1097
                     /locus_tag="PLF48_10935"
     CDS             72..1097
                     /locus_tag="PLF48_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNF49888.1"
                     /translation="MGLFSFLNKEIAIDLGTANTLIMYKDQVVVDEPSIVARDKKTEQ
                     YIAVGKKAMMMHEKTHANIETIRPLKDGVIADFSAAEAMLRGFIGMISDSNKWFRPSL
                     TMVLCIPSGITEVEKRAVFEGAEHANARERYLIFEPMAGALGIGLDVELPEGNMIIDI
                     GGGTTDIAVIALSGIVTDQSIRVAGDNFTMDIVEYIRRQHNILIGERTAEQVKIHVGS
                     SLQDLENAPEDYAVHGRDLLTGIPKQVTVSYTEIAGALDKSISKIEEAIMKALESTPP
                     ELASDIYRKGIYLTGGGALLRGLDKRIAMKTKLPVRVAEDPLRAVVRGTAIALKERDR
                     YRAILMS"
     gene            1102..1929
                     /gene="mreC"
                     /locus_tag="PLF48_10940"
     CDS             1102..1929
                     /gene="mreC"
                     /locus_tag="PLF48_10940"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNF49889.1"
                     /translation="MRGIIYLFTRFHVLLLFLVLEIFALSLVFKSHKYQEVALLNTSN
                     SISGKFLSWSNSISSFVNSGKNNKILSAENARLRSQIIYQDKYIKDTVLPLESDLYTY
                     NYIPAKIVNNTVNKSYNYITINKGSDDGLHKGNGVVSSNGVVGVVTNLSAHYALVMSV
                     ISKKSFVSVRHKNSNALGNLDWSGKNPFVLEVRNMSKTIPIKKNDTIVTAGFSSIFPP
                     DLMVGTVMEFKPDNSTGFLDIKIKPSNDIPSLSYVYIVKNTKKNELDSLQRLIQDEQ"
     gene            1919..2434
                     /locus_tag="PLF48_10945"
     CDS             1919..2434
                     /locus_tag="PLF48_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49890.1"
                     /translation="MNNKFILNNIFRFVILFVLQVFLFNQLPFGRIYIMIYPLAVLLL
                     PVFTNRSIVLLSAFTLGIMIDLFSDGGGLHTVSILLIAYLRSYVLDTFQPKSGWDKLD
                     VPTLNKQGITWYFYYTLILFSIHHLCFFYLEAFGFTNFFHTLFKTILSLFASLFIVWL
                     ISLFFMRNTKE"
     gene            2450..>3148
                     /locus_tag="PLF48_10950"
     CDS             2450..>3148
                     /locus_tag="PLF48_10950"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49891.1"
                     /translation="MQRGNESGILEKAIYIVATILLVRLLFLQVLSPTYKSMAKDNVI
                     KKQVVYPSRGLIVDRHGKVLVYNDAVYDILIQMNKTKNLDTALLCSLLNTQIEFVRKK
                     LDEISRTTPNKPTPFIKLVDAQTFAVFQEHLFQFPSFTYQLRTVRRYPYHSGAAVFGY
                     LSEVTEDMIAKSNGEYELGDYMGTTGVERYYDSLLRGEKGYNYQLVDVKNAYKGKYKD
                     GTEDKEPVSGWDMVT"
BASE COUNT          993 a          536 c          630 g          989 t
ORIGIN      
        1 tacatcccga atatcgttta taaattgtac atttgccctt tattcagtgc ataagaaaag
       61 aactatagaa aatgggatta ttcagttttc tcaataaaga aattgctatc gatttaggta
      121 ctgccaatac cttgattatg tataaagatc aagtagtggt ggatgagccc tctattgtag
      181 ctcgcgataa aaaaacagaa caatatattg ctgtaggcaa aaaggccatg atgatgcatg
      241 aaaaaacgca tgctaatatc gaaacaatac gcccgttgaa agatggcgta atcgcagatt
      301 tctctgctgc cgaagctatg ttacgcggtt ttatcggtat gattagcgac tccaacaaat
      361 ggtttcgtcc ttcacttaca atggttcttt gtataccatc cggtattact gaggtggaga
      421 aaagagccgt atttgaggga gcagaacatg ccaatgcgcg cgaacgttat ttgatatttg
      481 aaccgatggc cggtgcactg ggtattggtc tagacgtaga gttacctgaa gggaatatga
      541 ttatcgacat cggtggcggt actacggata tcgccgtgat tgctttatct ggaattgtaa
      601 cggaccaatc tatccgcgta gcgggagata atttcactat ggatattgtt gaatatatac
      661 gtcgtcaaca taatatttta attggagaaa gaacagcaga gcaagtaaaa atacatgtag
      721 gttcttcttt gcaggatttg gaaaatgccc ctgaagatta tgccgttcac ggtcgtgact
      781 tgttaaccgg aattccgaaa caagtaaccg tatcgtatac tgaaattgcc ggagcattgg
      841 ataaatctat ttcaaagatt gaagaagcta ttatgaaagc acttgaatct acaccgcctg
      901 agctggcatc ggatatctac agaaagggta tctatcttac aggaggtggt gctttgcttc
      961 gtggtttgga taagcgtatt gccatgaaaa ccaaattgcc ggtacgtgtt gctgaagatc
     1021 cgcttcgggc tgttgttcgc ggtacagcca tagcacttaa agaaagagac cgttaccgtg
     1081 ctattttgat gagttaatcc catgcgtggc ataatttatc tgtttacccg ttttcatgta
     1141 ctcctgcttt ttctggtctt agaaattttt gctttaagct tagtgtttaa atcgcataaa
     1201 tatcaggaag ttgctttact caacacgtct aactccataa gcggtaaatt tttatcctgg
     1261 tctaacagca tctcgtcttt cgtcaattca ggaaaaaaca acaagatatt gtcagctgaa
     1321 aatgcacgtt tgcgttctca aatcatttat caggataaat acattaaaga tactgtattg
     1381 ccacttgaat cagatttata tacgtataat tacatacctg ctaaaattgt aaacaatacg
     1441 gtgaacaaat cttataatta tatcaccatt aacaaaggca gcgatgatgg ccttcataaa
     1501 ggaaacggtg tagtcagcag taatggtgta gtgggtgtag tcaccaactt atcagcacat
     1561 tatgcactag tcatgtccgt aatcagtaaa aagtcatttg tcagtgtgag acataaaaac
     1621 tccaacgcat taggaaattt agactggagc ggaaaaaatc cgtttgtact ggaagtgcga
     1681 aatatgagta aaactatacc aattaaaaaa aatgatacga ttgtaacggc agggttttct
     1741 tccatatttc cacctgattt gatggtgggt acggttatgg aattcaagcc agataacagc
     1801 acaggatttt tggatattaa aataaaaccg tctaatgaca ttccatcatt aagctatgtg
     1861 tacattgtaa aaaatacaaa aaagaacgag ctggactcat tacaacgact tatacaagat
     1921 gaacaataaa tttattttaa ataatatttt tcgttttgtc atactttttg tattacaggt
     1981 atttttattt aatcaattgc cttttggtcg catttatatc atgatttatc cgttggctgt
     2041 attattattg cctgtattta caaacagatc tattgtttta ttgagtgcgt tcactttggg
     2101 aatcatgata gatttattca gtgatggcgg tggattgcat acagtatcaa ttttgctcat
     2161 tgcctatttg cgaagctatg tacttgacac attccaacct aaatcgggat gggacaaact
     2221 ggatgtgcct acgctcaata aacaaggtat tacctggtat ttttattata cacttatcct
     2281 gttttcaatc caccatttat gcttctttta tcttgaagca tttgggttta caaatttctt
     2341 ccacacgtta tttaaaacaa tattaagcct tttcgcttcg ttatttattg tgtggttgat
     2401 aagtttattt tttatgcgga atacaaaaga gtaatttcag gattagtaca tgcaaagagg
     2461 taacgagagc ggcattttag aaaaagctat ttatatagtt gcgacaatac tattagtacg
     2521 gcttttattt ttgcaagtgt tatcgccaac ttataaatca atggcgaaag acaatgtgat
     2581 aaaaaagcaa gttgtttatc cctcaagagg tttaattgtt gaccgtcatg gcaaggtctt
     2641 ggtttacaat gatgcggtat acgacatact gattcaaatg aacaaaacaa aaaatttaga
     2701 tacggcttta ttgtgttctt tactgaatac acagatagaa tttgtcagaa aaaagctgga
     2761 tgaaatcagc agaactacac caaataaacc aacccctttc atcaaattag tagatgccca
     2821 gacctttgca gtatttcaag aacatttatt tcagtttcct tcatttacct atcagcttag
     2881 aactgtaagg cgttatcctt atcattccgg cgctgctgta ttcggatatt taagtgaagt
     2941 aacagaagac atgattgcaa aatcgaatgg agaatatgag ctgggtgatt atatgggcac
     3001 tacaggcgta gagcgttatt atgacagcct attgcgaggt gaaaagggct acaattatca
     3061 gttggtcgat gtaaaaaatg catataaagg caaatataaa gatggtactg aagacaaaga
     3121 gcctgtttcc ggctgggata tggtgact
//
LOCUS       DAOQII010000283         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_21274_length_3071_cov_4.150531, whole genome shotgun sequence.
ACCESSION   DAOQII010000283 DAOQII010000000
VERSION     DAOQII010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21274_length_3071_cov_4.150531"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..856
                     /locus_tag="PLF48_10955"
     CDS             194..856
                     /locus_tag="PLF48_10955"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNF49892.1"
                     /translation="MIDSDSSVTLASGFKFDSLAIDSIIKTNAHDTVTSSAKEFKRTK
                     KQVHQLSDSAQQKKAITNKFGKEKITAKTQKTAKKAALYGLACPGLGQIYMRKYWKLP
                     IVYGLLGGGLYFVVSQGKQLKMFNGYLRDTLNNRPIPEPYNQLGPTELQTYRDYYRKN
                     TQLASFATIFIWGLAILDAAVDAHMSKFDISDNLSLQIAPKINTVGNQYYTGLSLNLT
                     LK"
     gene            894..1637
                     /gene="dapB"
                     /locus_tag="PLF48_10960"
     CDS             894..1637
                     /gene="dapB"
                     /locus_tag="PLF48_10960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNF49893.1"
                     /translation="MKIALIGYGKMGQTIHALAKEKNEKAGNNFYDVSLIVDIDNRPT
                     ITLDELRAVDVAIEFTSPHTAVENIKWCFEAGIPVVVGSTGWTEQLPEIKDIAIKTNQ
                     SFLFAPNFSIGVNIFFEVNRYLAEIMNKQTDYDVILEEIHHTEKKDAPSGTALHAALD
                     ILKRLARKKEWENNASDNEAILSIISKREPDVPGTHSITYENEIDKIELIHTAHSRKG
                     FAGGALTAAQWLIGRKGVYSMEDVLGFSK"
     gene            1712..>3071
                     /gene="lepB"
                     /locus_tag="PLF48_10965"
     CDS             1712..>3071
                     /gene="lepB"
                     /locus_tag="PLF48_10965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNF49894.1"
                     /translation="MLSLVYFYVLFYVLHFPLFNWYKKAGKNPFHVFIPLTQDFTLME
                     IIGRPKKQAYWGLVPYINMLFGLTWVPDICNSFGKRNFIHHFFAIMFSPLYFLVVGFF
                     PDLKYEGPAHTPDGEPKKNRSMGREWADALLFAFVAATIIRFFVIEAYKIPTTSLEGS
                     LLEGDFLFVSKLNYGARVPMTPIAFPLAHHTMPFGMGKAYSKLIELPYFRLPGLQDIK
                     NNDIVVFNYPGDAYENPERPVDKRENYVKRCVGIPGDDILIKDRELFVNGKHVPHFSK
                     MQFAYDIYTNKPFTIESLADLKIDWYKDILLGRYMDGLGQPVKPLDELKQEFTADMVN
                     NPHFIILLNDEQLAAIKKLPNVDSVKPINTADDVYQYHDSTLSVLFPNDTMDMRSGGY
                     ARYKWDLDNYGPIHIPKKGEVVTLSSDNIYFYYNIITTHEGNTLKMENGKFIINGKPE
                     TTY"
BASE COUNT          988 a          595 c          562 g          926 t
ORIGIN      
        1 atcatattta tcacaacaaa actgtatgtt attcactttc tactttacac ttatccctta
       61 catactgctc caggcatttg actaacaaaa tcaagctttc ttttttatat ttgtgtccga
      121 ataaatcaca ttgaaaaaaa tcgcatctgt ttgtattctt gttatgtgtt gcctttggct
      181 gcctgcgcaa actatgatcg attcggattc ctctgttact ctcgcctccg gtttcaaatt
      241 cgattcgctt gccattgaca gcattattaa aactaatgca catgataccg taacttcctc
      301 agctaaagaa tttaagcgaa caaaaaaaca agtacaccag ttgtcagatt cagcgcagca
      361 aaagaaagca ataaccaata aatttggcaa ggaaaaaatc acagcgaaaa ctcaaaaaac
      421 tgcgaaaaaa gcagccttgt atggtttagc ctgcccgggt ttaggacaaa tatatatgcg
      481 caaatactgg aaactgccca ttgtttacgg gcttttaggc ggcggtttgt attttgtcgt
      541 ttcacagggc aaacaactta aaatgtttaa cggctattta cgagatacac tcaacaatcg
      601 tcccattccg gaaccttaca accagcttgg tcctacagaa ttacaaactt accgcgatta
      661 ttaccgcaaa aacacgcaac tcgcatcctt tgctaccatt tttatctggg gcttggctat
      721 cctcgatgcc gcagtagatg cgcacatgag caagtttgat atttctgata atctctctct
      781 gcaaattgca cctaaaatta acacggtagg caatcaatat tatacgggat taagtttaaa
      841 tttgacactg aaataacgta attcgtcaac gagttgctct aaaattatac aagatgaaaa
      901 ttgcattaat cggttatggc aaaatgggac aaaccataca tgcattagcg aaagaaaaaa
      961 atgaaaaagc tggcaacaac ttttatgatg tatccttaat tgtggatatt gacaacagac
     1021 ctactattac actggatgaa ttgcgtgcgg tagatgttgc tatagaattt acctcaccgc
     1081 acaccgctgt tgagaatata aaatggtgtt ttgaagccgg cattcctgtg gtagtcggct
     1141 caacaggatg gacagaacaa cttccggaaa ttaaagacat tgctataaaa actaatcaat
     1201 catttttatt tgctccgaat ttcagtattg gcgttaatat tttttttgaa gtcaaccgct
     1261 atctggcaga aattatgaat aagcaaactg actacgatgt tattttggaa gaaatacacc
     1321 acaccgaaaa gaaagatgct ccaagtggca cggcattgca tgctgcatta gatattctga
     1381 aaagattagc acgcaaaaaa gaatgggaaa ataatgcatc tgacaacgag gctatattga
     1441 gtattatttc taaaagagaa cctgacgttc cgggtacaca cagcattact tatgaaaatg
     1501 aaatagacaa aatagaattg atacacactg cccattcccg caaaggtttt gccggagggg
     1561 cacttaccgc tgcacaatgg ctgattggca gaaaaggcgt gtacagcatg gaagatgtgt
     1621 tgggcttttc aaaataaggc atttaacatt tcttcaccat gatattgtaa gatatgctta
     1681 cctttaatca cttattcaat aaaaaaaata tatgctcagt ttagtttact tttatgtact
     1741 gttttacgtt ctgcattttc cattattcaa ctggtataaa aaagccggaa aaaacccatt
     1801 ccatgtattc attccgctta cacaggattt cacattgatg gaaatcatcg gaagacctaa
     1861 aaaacaagct tattggggtt tagttcccta tatcaatatg ctttttggat taacctgggt
     1921 gccggatatt tgtaattcat ttggcaaaag aaatttcatt caccatttct ttgctattat
     1981 gttcagtccg ctctattttt tagttgttgg attttttcca gatttaaaat atgaaggtcc
     2041 cgctcatact cccgatggtg aacctaagaa gaatagaagt atgggacgcg aatgggctga
     2101 tgcactttta tttgcttttg tagcagctac tatcattcgt tttttcgtta tcgaagcgta
     2161 taaaattccc accacttcgc tcgaaggttc tttgctggaa ggagatttct tatttgtaag
     2221 caagcttaac tatggcgcgc gtgtgcccat gacacctatt gcgtttcctt tggctcatca
     2281 cacgatgccc ttcgggatgg gaaaagctta ttccaagctg atagaactcc cctatttcag
     2341 attacccggt ttgcaggata taaaaaataa tgacatcgtg gtgtttaatt atcccggtga
     2401 tgcttatgaa aaccccgaaa gaccggttga caagcgcgaa aactatgtaa aacgttgtgt
     2461 gggaattccg ggtgatgata ttttgataaa agacagagaa ttatttgtta acggcaagca
     2521 tgttcctcat ttttccaaaa tgcagtttgc ctatgatatt tatacaaaca aacctttcac
     2581 tatcgagtcg ctagctgatt taaaaatcga ctggtataaa gatattttat taggtaggta
     2641 tatggatggc ttaggtcaac ccgtaaaacc attagatgag ttgaaacaag aatttacagc
     2701 agatatggta aacaatccgc attttattat cttgttgaat gatgagcagc ttgcagccat
     2761 taaaaaactt cccaacgtag attcagtaaa acccatcaat actgcagatg atgtttatca
     2821 atatcacgac tccacacttt ctgttttatt tccaaatgat actatggaca tgcgtagtgg
     2881 cggttatgct cgctataaat gggatttaga caattacggg cccattcata ttcctaaaaa
     2941 aggcgaagta gtcacactca gcagtgataa tatatatttt tattacaaca ttattaccac
     3001 tcacgaaggc aataccttaa agatggaaaa cggaaaattt atcatcaacg gaaaaccgga
     3061 aactacatat a
//
LOCUS       DAOQII010000284         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_21416_length_3059_cov_4.582889, whole genome shotgun sequence.
ACCESSION   DAOQII010000284 DAOQII010000000
VERSION     DAOQII010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21416_length_3059_cov_4.582889"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PLF48_10970"
     CDS             <1..1127
                     /locus_tag="PLF48_10970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNF49895.1"
                     /translation="IYGNVISNVKTENVAREAAVTAGIPVKAPCNTVKQACISANQAI
                     SNAMDSIQLGRADVIISGGTECTSDTPIGYTKEMRKKLFAAQKLSSPMEYVKFATTLR
                     PNDFLPDRPAVAEFITGRTMGQDCEILAQKFGVTREEQDELAAASHQNAGKAFDAGYM
                     DDIIDVRLPPKFTPISKDNGVRGDTTVDKISKLKPAFDKYVGTITAANASFLTDGATA
                     VLLTSEEKAKELGLKPKAELVDYVFTGADLYDELLLGPAYSIAELLKRNKLKVEDIDV
                     WEIHEAFAGQVLANLKALENEEWCKSHVGYEGAVGSIPKEKINAWGGSLSIGHPFGAT
                     GGRLLTMAANRIQKQGGKYAILAACAAGAHGTAMLIKKWD"
     gene            1222..1596
                     /locus_tag="PLF48_10975"
     CDS             1222..1596
                     /locus_tag="PLF48_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF49896.1"
                     /translation="MIKSNVTYKNLILWQHSMELVTSIYTVTKTFPESEKFKLISQFN
                     RAVVSIPSNMAEGYGRASAKNYIQFLKISIGSVYKVETLLLISKKLNYLSSEKYIKFQ
                     NLLEEISELLNGLISSIQRKIA"
     gene            1649..>3059
                     /locus_tag="PLF48_10980"
     CDS             1649..>3059
                     /locus_tag="PLF48_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNF49897.1"
                     /translation="MRQQDYISVQKSPNGVCTIWMDQKNEKINKVGPDLIQLFEDVFT
                     ELDKDDSIKAFVINSKKKDFIAGADIEMFQKVKNKGDFEPITRKGHAILNKVAASKKP
                     IVAAINGNCLGAGLEIALACHARVCSNDKSTKMALPEVKLGLLPGGGGTQRLPRLVGL
                     QAALDMMLTGKNIFPYPALKMGLVDKVVHVNALQATAEKLALEVAAKPIKRERNDINM
                     NTVRAGFPALQSKLMNMVMEAPLVNKIVFDQAKKMVDKQAHGNYPAPYKILECVETGW
                     NQGMEKGLDAEAVKFEELLLGDVSKQLINIFFAMTDKKKNPYDEKLIKKVHRMGIIGA
                     GFMGAGIAEVSIDDGMHVLLKDVNQDMINSAFKTIYESNNKRVLKKAMTKIELEEKMA
                     LLSGSLNYEDLDNQEIIIEAVFEDLNLKQKIVADAEANAKPNTIFASNTSALPIKEIA
                     AKAKHPELIIGMHYFSPVPK"
BASE COUNT         1067 a          544 c          619 g          829 t
ORIGIN      
        1 taatatatgg aaatgttatt tctaacgtaa aaacagaaaa tgtagcccgt gaagccgctg
       61 taaccgccgg aattccggta aaagctcctt gcaataccgt gaaacaagct tgtatctctg
      121 ccaatcaggc aatatctaat gcaatggaca gcattcaatt aggcagggca gatgtaatca
      181 tctccggtgg cacagagtgc acttctgaca cgcctattgg ctatactaaa gaaatgcgca
      241 aaaaactatt tgcagctcaa aaactgtcga gcccgatgga atatgtgaaa tttgctacta
      301 cgcttcgtcc caatgatttt cttcctgaca gacctgctgt tgctgagttt atcaccggac
      361 gaactatggg tcaggattgt gaaatattag ctcaaaaatt cggagttacc cgtgaggaac
      421 aagatgaact ggcagcagcc tctcatcaaa atgcaggcaa agcctttgat gccggttaca
      481 tggatgatat tattgatgtg cggttaccac ctaaatttac gcctatttct aaagacaatg
      541 gagttcgcgg tgataccact gtcgataaaa tttcaaaact aaaacctgcc tttgataaat
      601 atgtgggtac gattactgca gcaaacgctt cttttttgac agacggtgca actgccgtat
      661 tactgacatc agaagagaaa gcaaaagaat taggcttaaa acctaaagca gaattagttg
      721 actatgtatt tacaggtgct gatttatatg atgaattact cttaggacca gcctactcca
      781 tagctgaact tttgaaaaga aacaagctta aagtggaaga tattgatgtt tgggaaattc
      841 acgaagcatt tgccggacaa gtgcttgcca acttgaaagc tttggaaaat gaagaatggt
      901 gcaaatcgca tgtaggttat gaaggggcag taggcagcat tccaaaagaa aaaataaatg
      961 cttggggtgg ctcattatcc attggtcatc catttggagc taccggagga agactactca
     1021 ccatggctgc taaccgcata caaaaacaag gtggaaaata tgccatctta gccgcttgcg
     1081 ccgcaggggc acacggcaca gcgatgttga ttaagaagtg ggattagtaa ttagtaatta
     1141 gcaattagtg atttgtgatt tgtgatttgt aatatgttat aatataattt tttttgatag
     1201 ttattttaac aaggcaattt tatgattaaa agtaatgtaa cttataaaaa tctaattttg
     1261 tggcagcatt caatggaact tgttacaagt atatatacag ttactaagac atttccagaa
     1321 tcagaaaaat tcaagttgat atcccaattc aatagagctg tagtttcaat tccttcaaat
     1381 atggcagaag gatatggcag agcaagtgca aagaactata ttcaattcct taaaatttca
     1441 ataggatctg tttataaagt tgaaacttta ctactaattt caaaaaaact aaactattta
     1501 tcttctgaga aatacataaa atttcaaaat ttattagaag aaatttcaga attattgaat
     1561 ggattaattt caagtattca aagaaaaatt gcataatcac ttatgcctcg tgccaagtgc
     1621 ctattgccta aaaacaaata aacaatttat gagacaacaa gattatatct ctgtacaaaa
     1681 atctcccaat ggtgtatgca ctatctggat ggatcagaaa aatgaaaaaa tcaacaaagt
     1741 gggtcctgat ttgattcagc tctttgaaga tgtttttacg gaattagata aagacgattc
     1801 catcaaagct tttgtcatca acagtaagaa aaaagatttt attgccggcg cggatattga
     1861 gatgtttcaa aaggtaaaaa acaagggcga ctttgaaccc attacacgta aaggtcatgc
     1921 tattctgaac aaagtagcag catccaaaaa gcctatcgtg gctgcgataa acggcaattg
     1981 tttaggtgca ggactggaaa ttgcactggc ttgccatgcg cgggtttgca gtaatgacaa
     2041 atccaccaaa atggctttgc ccgaagtaaa attgggactg ttgccaggcg gtggcggcac
     2101 acaaagacta ccgcgcttag tgggcttaca agcagccttg gatatgatgc tcacaggtaa
     2161 aaatattttc ccataccctg cattaaaaat gggcttggtg gacaaagtag tacatgtaaa
     2221 tgccttacaa gctacagccg aaaaactggc attggaagtt gccgctaaac ctataaaaag
     2281 agaacgcaac gacatcaata tgaataccgt tagagccggt tttcctgcct tacaatctaa
     2341 attgatgaat atggtgatgg aagcgccact ggtgaataaa attgtcttcg atcaggcaaa
     2401 aaaaatggtg gacaaacaag cacatggcaa ctatcccgct ccttacaaaa ttttagaatg
     2461 cgtagaaaca ggctggaatc aaggcatgga aaaaggactc gatgcagaag ccgtaaaatt
     2521 tgaagaatta ttacttggcg atgtatctaa acaactcatc aatatctttt ttgccatgac
     2581 ggacaaaaag aaaaatcctt atgacgaaaa actgattaaa aaagttcacc gtatgggcat
     2641 cataggtgca ggttttatgg gtgccggcat agccgaagtt tcaatagatg atggcatgca
     2701 tgtactactc aaagatgtca accaggatat gattaattcg gcattcaaaa ccatttatga
     2761 aagcaataat aaacgtgttt tgaaaaaagc catgaccaag atagaactag aagaaaaaat
     2821 ggcactttta tcaggcagtt tgaattatga agacttagac aatcaggaaa ttataataga
     2881 agctgtgttt gaagatttga atttgaaaca aaaaatagta gcggatgcag aagccaatgc
     2941 aaaacctaat accatattcg cttccaatac ttcagcattg cctatcaaag aaatagcagc
     3001 aaaagccaag catcccgagt tgattatagg aatgcattat ttttctcccg tgcctaaga
//
LOCUS       DAOQII010000285         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_21515_length_3050_cov_5.709182, whole genome shotgun sequence.
ACCESSION   DAOQII010000285 DAOQII010000000
VERSION     DAOQII010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21515_length_3050_cov_5.709182"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLF48_10985"
     CDS             <1..477
                     /locus_tag="PLF48_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49898.1"
                     /translation="HSKFDWGMPFAGGGNMANFMNNATAGGDSYRIGNSGPMTAGGPI
                     AVTISRGFLAWVLADESITEPTDAFVPKHIVYVIGSTVAIGVISFYGQSINWDQFRFT
                     KGGKQNIYDDELSPLSDEALKALEQAAKVAKNTQLLQKIIKEWKRRAVRNNQKQRK"
     gene            483..1010
                     /locus_tag="PLF48_10990"
     CDS             483..1010
                     /locus_tag="PLF48_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49899.1"
                     /translation="MSNNLGGNKFVRSEFYEKLNIYFFSTNNNIIENIFDIAISERLF
                     NVKIIELCERVLSRRFTYLTKVTALDYIAILHKKIDKNTFIRLNQIHFKSQNDILKFQ
                     SIINLMIYDYYLYEKSIGKMLNKTDYPTLFYRLRNNLENFPKSKKSQLNIILLNSLKD
                     KKFSDDVKNDFFRNI"
     gene            complement(1585..2661)
                     /locus_tag="PLF48_10995"
     CDS             complement(1585..2661)
                     /locus_tag="PLF48_10995"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNF49900.1"
                     /translation="MHIGLVFGGKSPEHEVSIVSARNIYNAIDKNKHRVTLIGIAADG
                     KWYAETEENLQDKSCVVGKSGVQLAVVFGNEKNKIIRLANAAFLDDLEVIFPITHGPN
                     GEDGTLQGIFTQMDIPFVGPGVLGSAVAMDKDFTKRILRDAGIGHAKGMVFYKHERAE
                     ISYDKVQAELGNLLFVKPCNMGSSVGVSKCTNATEFNTALDLAFRFDTKVLVEQGING
                     RELECAVFGNENPKVSTAGEIKMLQGFYDFENKYVNDNAQITIPAEHLSEAQLTKVRA
                     TALRAYQVLGLEGMSRVDVFLTADDEVIINEPNTLPGFTSISMYPKLWENSGVSYADL
                     IEQLIGFAIARHDRDKQIQRVRVV"
     gene            2765..>3050
                     /locus_tag="PLF48_11000"
     CDS             2765..>3050
                     /locus_tag="PLF48_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49901.1"
                     /translation="MKNMLILFLLLSNSCLFAQKNYQLFQPNLTRNVIIYTPRNFNPN
                     ENLPLVINMHGFLTTNSFQMDYTKFNNTADTARCIVAYPQGKDLRWNSGTF"
BASE COUNT         1060 a          567 c          500 g          923 t
ORIGIN      
        1 cacagtaaat ttgattgggg aatgccattt gcaggcggcg gtaatatggc gaattttatg
       61 aataatgcta cggctggcgg tgactcttat agaattggta atagtggacc aatgacagca
      121 ggtggaccta ttgcagttac tatatctcga ggatttttag catgggtttt ggctgatgag
      181 tctattactg aacctactga cgcatttgtg cctaaacata ttgtttatgt aattggctca
      241 acggttgcca taggtgtaat atctttttat ggtcaaagta taaactggga tcaatttaga
      301 tttactaaag gcggtaaaca aaatatatat gatgacgaat tatctcctct ttctgatgaa
      361 gcacttaaag cattagaaca agcagctaag gtcgcaaaaa acactcaatt acttcaaaaa
      421 ataattaaag agtggaagcg acgagcagta agaaataatc aaaaacaaag aaaataaatt
      481 ttatgagcaa taacttaggt ggcaataaat ttgtaagatc agaattttat gaaaagttga
      541 acatttattt cttttctact aataataaca taattgaaaa tatatttgat attgctatat
      601 cagaaagact gtttaatgtg aagattattg aattgtgtga gagagtactc tcaagaagat
      661 ttacttacct tacaaaggta actgcattag attatatcgc aatattgcac aagaagattg
      721 ataagaatac ttttataagg ttaaatcaga tacatttcaa atctcaaaat gatattctga
      781 aatttcaatc cattattaat cttatgattt atgattatta cttatatgaa aaaagtattg
      841 gtaaaatgtt aaataaaact gattatccga cattatttta cagattaagg aataatttag
      901 aaaattttcc aaaaagtaaa aaatcacaat taaatattat actgttaaat tcattaaaag
      961 ataaaaaatt tagtgatgat gtaaaaaatg acttttttag gaacatttaa gtatagaaaa
     1021 gataaatatt cccccggctc tcgcaagtac aatttcatta agttaagaaa ataacagttg
     1081 ataataagtt tgctcgccct cgcccctgac aagtgttatc acctgccaac acgaaccata
     1141 ataaaaccgg aaaatatttt tttatttcaa gtatgattta catgctcgca atgctgtagc
     1201 aagacaagca gacgacaacc ttgaaagtag ctaattgtat aattgagaaa aacagtatct
     1261 ttaaataaca aaagttcttt gaatgatgtt gcaacttggt acgaacaata gcagcatgcg
     1321 gcgaataaaa aatcgcagcg acttaaaact atcgtgcata cacaaaagaa atgcaatgtg
     1381 ttgtaaaaaa agaagtaagc aataaaacaa gcacaaatga aaacgacaag atagttttta
     1441 gcaagcagat tttttagagc tgaagccacc agccaagcaa aaatgcctct attgttcttg
     1501 attttttgct ccacttttgt atcaagacaa aagtggaaaa aatagttctt tgaaaacttc
     1561 cgaaaaagaa ataaaaccac tcagctacac cacccgtacc cgctgtattt gtttatctct
     1621 atcatgccgg gcaatagcaa aaccaataag ctgttctatt aaatcggcat aagacacacc
     1681 actgttttcc cacaacttgg gatacatcga aatagaagta aaaccgggca aagtattggg
     1741 ctcattgata atcacttcat cgtcagcggt caaaaataca tccacacggc tcatgccctc
     1801 cagtcccaat acctgataag cacgtagtgc agtagctctt actttagtaa gttgcgcttc
     1861 actaagatgt tcagcaggaa tagtgatttg agcattgtca ttcacatatt tattctcaaa
     1921 atcataaaaa ccttgcagca tttttatctc gcctgccgta ctcactttgg gattttcgtt
     1981 gccaaaaaca gcacactcaa gctctctgcc atttatacct tgctctacca ataccttcgt
     2041 atcgaaacga aaggctaaat ccagcgcagt attaaactca gtggcattcg tgcattttga
     2101 aacgcccaca ctcgaaccca tattgcaagg ttttacaaag agcaaattgc ccagttcagc
     2161 ctgcacttta tcatagctga tttcagctct ttcatgttta taaaatacca taccttttgc
     2221 gtgcccaata ccggcatcac gcagaatgcg tttagtaaaa tctttgtcca tcgccacggc
     2281 agaacccaac accccgggac caacaaatgg aatatccatt tgtgtaaaaa taccttgcag
     2341 tgtgccgtct tctccgtttg gcccatgggt aatcggaaaa atcacctcca aatcatctaa
     2401 gaaagcggca ttagctaagc ggattatttt atttttttca ttgccaaaaa ccacggcaag
     2461 ctgtacaccc gatttgccga ctacacaact tttatcttgt aaattttctt cggtttctgc
     2521 ataccatttg ccgtcggcag caatgccgat aagcgtaaca cgatgtttat ttttgtcgat
     2581 agcattataa atattgcgcg ccgaaacaat agatacttca tgttcaggac tttttcctcc
     2641 aaatactaat ccgatatgca tgtgttgatt ttttgacaaa aataattcat tgtcaactac
     2701 tttttcaaac aaaattcaga tatccgaaat ttacactacc ttagaatcga aaaacttgac
     2761 gcacatgaaa aatatgctta tacttttttt attattgtct aacagttgtt tatttgctca
     2821 aaaaaattac cagctatttc agcctaatct cacacgaaat gtaattattt atacaccccg
     2881 caatttcaat ccgaatgaaa acttgccctt agtaattaat atgcatggtt ttctcactac
     2941 gaatagtttt caaatggatt atacgaaatt taataatacg gcagatacgg ccagatgtat
     3001 agtggcctat ccgcagggta aagatttgcg ttggaactca ggaacatttt
//
LOCUS       DAOQII010000286         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_21559_length_3047_cov_4.038102, whole genome shotgun sequence.
ACCESSION   DAOQII010000286 DAOQII010000000
VERSION     DAOQII010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21559_length_3047_cov_4.038102"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLF48_11005"
     CDS             <1..227
                     /locus_tag="PLF48_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49902.1"
                     /translation="IFWFTFSDMSHLTRADQINSGKMSLFAKIYHNLLDRGIYFGPSG
                     YEVGFVSAAHTDEDLDKAASLLNDAFDSIH"
     gene            complement(258..1571)
                     /gene="der"
                     /locus_tag="PLF48_11010"
     CDS             complement(258..1571)
                     /gene="der"
                     /locus_tag="PLF48_11010"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNF49903.1"
                     /translation="MSFTVAIIGRPNVGKSTFFNRMLQQRKAIVDDFSGVTRDRQYGI
                     CDWNGKTFNLIDTGGFVSATDDIFEKEIKKQVKIAIQESNLILFMVDVTTGITDLDES
                     LAQELRKSKKPVFLIVNKVDNIERGYAANEFYSLGFDQLFPVSSISGSGTGEILDEVT
                     AVIPPEHAEAIDEYASLPKIAIVGQPNVGKSTLLNALIGEERNIVTDIAGTTRDSIHT
                     HYNLFGKEFLLIDTAGIRKKNKVHEDLEFYSVIRAVKAIDEADVCVLMLDAQTGIEMQ
                     DLKIFSIIQNRKKGVVILVNKWDTIEKDNKTVGEYTRIIKQKIAPFSDVPIIFISAKD
                     KQRIFQAVEKILEVYNGMQIKIHTNKLNEIMLAEIEKYPPPAYRGEYIRIKYITQLPT
                     KSPSFAFFCNHPDYVREPYRNYLENRMREHFPLSGVPIQIYFRKK"
     gene            1677..2252
                     /locus_tag="PLF48_11015"
     CDS             1677..2252
                     /locus_tag="PLF48_11015"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNF49904.1"
                     /translation="MMQDTAITCPWCKTYYAVAPNYTNCRNCGGPLPKPISKERGERP
                     TAAPRVLPEKFKRQVRYWKNTFSLVGIIFMIIGTPLILAFGFGLIFVVIGYFLFRRGK
                     RTANETILALENGMSAEGIIEQIDLDYSQSINGKHPYRVRYNYQTSNGLHTDEIVCWD
                     EQNRWRNAGDEIWVVYMPENPEISSPWPPIV"
     gene            2331..>3047
                     /locus_tag="PLF48_11020"
     CDS             2331..>3047
                     /locus_tag="PLF48_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49905.1"
                     /translation="MKTRHHLKNLLIIMSTLLLGFTSCKKTDAILDESTAETLSVADL
                     EDDNVQAIADQAYETGNVSLRTSASSETNLINGCAIVTRDSSGTTHTLTIDFGTGCTG
                     AHGVTRKGKIIVTFTGPYREQGTVIHTTTDNYYVNNNKVDIDRTATNMGRNADENLYF
                     NVSSTRTITFADGTTISGTATRVREWIAGENTRDNFDDDVYRVNGTGTFTNRRGISGS
                     FSTQTTLIRKVACHQFVEGVV"
BASE COUNT          941 a          605 c          567 g          934 t
ORIGIN      
        1 ctattttctg gtttaccttt tctgacatga gccatcttac ccgtgccgac caaatcaatt
       61 cgggaaaaat gtccttattt gccaaaatct accataactt gttagaccgc ggtatttatt
      121 ttggtccttc aggatatgaa gtaggatttg tgtctgctgc acatacagac gaagatttgg
      181 acaaagccgc aagtttattg aacgatgcat ttgattccat tcattgacaa cctgagttta
      241 ggtaaaaaag taatttctta tttttttctg aaataaatct gaataggtac gccactcagc
      301 ggaaagtgtt ctcgcatgcg attttccaga tagttgcggt aaggctcacg cacataatca
      361 gggtgattgc agaagaaggc aaaacttgga gatttagtag gtagttgagt aatgtatttt
      421 atacgaatat actcaccgcg ataagccgga ggtggatatt tttctatttc agccagcata
      481 atttcgttca gtttattcgt atgtatctta atttgcatac cattataaac ctctaatatt
      541 ttttctactg cctgaaaaat gcgttgctta tccttagctg aaataaaaat gatgggcaca
      601 tccgaaaagg gtgcaatctt ttgcttgatg atacgggtat attcacctac ggttttatta
      661 tccttctcta tcgtatccca tttgttcacc agaataacta cccctttttt tcggttttgt
      721 ataatactga aaatctttaa atcctgcatt tcaatgccgg tctgagcatc cagcatcagc
      781 acacatacat ctgcttcgtc aatggcttta actgcacgga ttactgaata aaactccaaa
      841 tcttcatgta ccttgttttt tttgcgtatg cctgcagtat ctatcagcaa aaattctttt
      901 ccgaataagt tataatgcgt atgtattgaa tcacgtgtag tacctgctat atcggttacg
      961 atattgcgct cctctcctat cagtgcgttc agcaaggtag attttccaac attaggctgc
     1021 cctacgattg caatttttgg caatgatgca tattcatcta ttgcttctgc atgttccggc
     1081 ggaatgactg ccgtaacttc atccagaatt tctccggttc cgcttccact gatggatgaa
     1141 accggaaata actgatcaaa tccgagtgaa taaaattcat tggcagcata tcctctctca
     1201 atattatcca ctttattgac aatcaaaaaa acaggctttt tagatttgcg caattcctgt
     1261 gccagcgact catccaaatc agtaatgcct gtcgtaacat ccaccataaa caagattaaa
     1321 ttagattctt gtatggctat tttaacttgt tttttaattt ctttttcaaa aatatcatct
     1381 gtagctgaaa caaaacctcc ggtatcaata agattgaatg tttttccatt ccaatcgcaa
     1441 ataccatatt gccgatcgcg tgttactcca ctaaaatcgt caacgatggc ttttctttgt
     1501 tgtaacattc tattaaaaaa tgttgatttc cctacgttag gtcttcctat gatggctact
     1561 gtaaatgaca cgatttaatt tttttgcaaa agtaagcata tttaaacaat aagctctgat
     1621 taattaccgc ctgatttcat tgatattctt gtatctttaa tgaaaccaaa aaaacaatga
     1681 tgcaggatac agccattact tgtccatggt gtaagacgta ttatgccgtt gcacccaatt
     1741 ataccaattg ccgaaattgc ggtggaccct tacccaaacc catcagtaag gaacgcggag
     1801 aaagaccaac agcggcaccg agggtattac cagaaaaatt caaaagacaa gtgcgctact
     1861 ggaaaaatac tttttcgctg gtgggcatca tttttatgat aatcggcaca cctttaatat
     1921 tggcttttgg attcggacta atattcgtag taattggtta ttttttattc agacgaggaa
     1981 aacgcaccgc caacgagact atcttagccc tggaaaacgg catgagcgcg gaaggtatta
     2041 ttgagcaaat tgatttagat tattcacaat ccataaatgg aaaacatcct tacagggtaa
     2101 gatacaatta tcagaccagt aatgggctac atacagatga aatagtatgc tgggatgagc
     2161 agaacaggtg gagaaatgcc ggagatgaaa tttgggtggt ctatatgcct gaaaatccgg
     2221 aaataagttc accatggcca ccaatagtat aaaatatgtt agttttattt ctctttacaa
     2281 ttaaactttt caactttcgt ttagtcaaaa ggctatcaaa atttataatc atgaaaacac
     2341 gacaccactt aaaaaatctg ctcatcataa tgagcacact cttactggga tttacctcct
     2401 gtaaaaaaac agatgcaata ttggatgaat caactgcgga aacactgagt gtagcagatt
     2461 tagaagatga caacgtacag gcaattgctg atcaggctta cgaaaccggc aatgtcagtt
     2521 tacgcacttc ggcatcctct gaaaccaacc tgattaatgg ctgtgctata gtaaccagag
     2581 attcttccgg tacgacacat accttgacta tcgacttcgg aaccggttgt accggtgctc
     2641 atggtgtaac tagaaaagga aaaatcattg ttacttttac aggaccttat cgtgagcaag
     2701 gtactgtgat ccataccact accgataact attatgtaaa taacaacaaa gtagatatag
     2761 acagaacggc aactaatatg ggaagaaatg ctgatgaaaa tttatatttt aatgtcagct
     2821 ctacacgtac tatcaccttt gccgacggca ctaccataag cggcacagcc acacgtgtaa
     2881 gagaatggat agccggagag aatactcgcg ataattttga tgatgatgtt tatcgggtaa
     2941 acggaacggg tacttttact aaccgcagag ggataagtgg aagtttcagc acacaaacaa
     3001 ctttgatacg caaagtggcc tgtcatcagt ttgtagaagg tgtagta
//
LOCUS       DAOQII010000287         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_21582_length_3045_cov_4.445819, whole genome shotgun sequence.
ACCESSION   DAOQII010000287 DAOQII010000000
VERSION     DAOQII010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21582_length_3045_cov_4.445819"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLF48_11025"
     CDS             complement(<1..886)
                     /locus_tag="PLF48_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF49906.1"
                     /translation="MLKKVSLVIKAIFVIGLLFFIASFLGKYFWFFDLISHFRIQALV
                     MFALCLSYFIIIRENVFIVFNVSVVLFILYSIFPYYFPQPKYRADTTYTLLYANVLTS
                     NEQNSLLVSMINNTNPDIIALVEVNEEWMYQLNCIAKEYQYKIVLPHDDNFGVCLLSK
                     IDISESEVINFCTLDLPSVFAKLKTKDDSFSILITHTIPPVGHFDFIQRNLHLSRLHA
                     FAANNHVNMVVGDLNCSSFSPNFNILLNDNMLRVSRLGFGLQPTWPTWCPLFYTTLDH
                     IIVDEHLLVCDRKVLQNIG"
     gene            complement(923..2410)
                     /gene="guaB"
                     /locus_tag="PLF48_11030"
     CDS             complement(923..2410)
                     /gene="guaB"
                     /locus_tag="PLF48_11030"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNF49907.1"
                     /translation="MESNFHLSPQHKFAGEGLTFDDVLLLPAYSQVLPRETNLKTQVT
                     RNISANIPILSSAMDTVTEANLAIALAREGGIGVLHKNMSIEKQANEVRKVKRSESGL
                     ITDPITLNPEQKVAAAFILMAENKIGGIPVTDENGYLAGILTNRDLRFCKDKNMLVKD
                     LMTKDRLITAHEGTTLKQAEDILHEYRIEKLPIVDKNNKLVGLITYKDIIKVKNYPNS
                     CKDNSGRLRAAAAVGVTADFMQRMEALVAVGVDFICIDTAHGHSMGVINAVKQAKTSF
                     KYVDIVGGNIATGSAAKALADAGADAVKVGVGPGSICTTRVVAGVGVPQLSAIYDCAL
                     ALKGSGVPVIGDGGIRYTGDIVKALAGGANTIMAGSVFAGTEESPGSTVILEGRKFKT
                     YRGMGSMEAMQEGSKDRYFQDVEDDIKKLVPEGIVGRVPYKGTLSEVVYQFIGGLKAG
                     MGYCGAKDIPALQEKSQFVRITTAGVAESHPHDVTITNEAPNYSR"
     gene            complement(2474..>3045)
                     /locus_tag="PLF48_11035"
     CDS             complement(2474..>3045)
                     /locus_tag="PLF48_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49908.1"
                     /translation="KAFSQFLMPRLAACEHRRKMSEHKSGIFRQFDVSAAFSILRTHE
                     QPDNFSPKRAGTKSICMHNNGLFCPHQTTGSMVAVLRKNNLPHTVWFTGSSNPCISLY
                     KPFYFGDTLLNEEQFQQPGAHADDSYWWKWEIRNRKFRNDFIHQWENFHTTQQHLEAE
                     WIKEDALFTSMSDLEKGYQLSRKALAVTV"
BASE COUNT          900 a          657 c          568 g          920 t
ORIGIN      
        1 agcctatatt ttgtaggact ttcctatcac aaacaagcag atgttcatca actataatat
       61 gatccaaggt tgtataaaac agtggacacc aagtaggcca tgtaggctgt aacccgaacc
      121 ctaagcggct gactctaagc atattatcat ttagtaatat attgaagttc ggagaaaatg
      181 aagagcaatt aagatcacca accaccatat ttacatgatt atttgcggca aatgcatgaa
      241 gtcgggagag gtgcaagttt ctttgaataa aatcgaaatg acctactggc ggtatagtat
      301 gtgttatcag tattgaaaac gaatcatctt ttgtttttag cttagcaaat actgaaggta
      361 aatctaatgt gcaaaagttt atgacctcac tttctgagat atcaatttta gacagcaaac
      421 atacaccgaa attatcatca tgaggtaaca caattttata ttgatattct ttagcaatac
      481 aatttaattg atacatccac tcctcgttaa cttccaccag cgcgatgata tcgggatttg
      541 tattattaat catactaaca agcaagctgt tttgctcatt agaagtaagt acgtttgcat
      601 aaagtaatgt gtatgtcgta tcggctctat attttggctg tggaaaatag tacgggaaaa
      661 tgctgtataa aataaaaaga acgacagaaa cattaaatac aataaagaca ttttctctga
      721 taattataaa ataagacaaa cacaatgcaa acatgacaag agcttgtatt ctgaaatgac
      781 tgatcaaatc aaaaaaccaa aaatattttc ccagaaaaga tgcaataaag aacaggagtc
      841 ctataacaaa aattgcttta attacaagcg ataccttctt taacataact atcttgaaaa
      901 ataaaaatag ttagaattca cattatctgc tataattcgg tgcttcattg gtgatggtta
      961 catcatgcgg gtggctttcg gctacgccgg ctgtagtaat acgcacaaac tgcgattttt
     1021 cttgcagtgc aggaatgtct tttgctccgc aataacccat acccgccttt aatccgccta
     1081 taaactgata aacaacttcg ctcagcgtac ctttatacgg cactctgcct acgatgcctt
     1141 cgggcactaa tttttttata tcgtcttcca catcctgaaa atagcggtct ttactgcctt
     1201 cttgcatagc ttccatactg cccataccgc gataggtttt gaattttctt ccttcaagta
     1261 taacggtgct gccggggctt tcttccgtgc cggcaaatac cgaacccgcc ataatggtat
     1321 tggcaccacc tgccagtgct tttacaatat ctcccgtata gcgaataccg ccatcgccga
     1381 taacaggcac cccactgcct tttaatgcca gtgcacaatc gtaaatggca ctcagctgtg
     1441 gcacacctac tccggcaaca acacgtgtag tacagataga acccggacca acacctactt
     1501 ttacagcatc agcaccggca tcggcaagtg cttttgctgc cgaacccgta gcaatgtttc
     1561 cgcccacaat atctacatat ttaaaactgg ttttagcttg ttttacggca ttaataacgc
     1621 ccatagaatg accatgtgcc gtatctatac aaataaaatc aacacctacc gccaccagtg
     1681 cttccatacg ctgcataaaa tcggcagtaa cccctacagc agctgcggca cgcaatcgtc
     1741 cgctgttatc tttgcaggaa ttgggataat tttttacttt aataatatct ttatacgtaa
     1801 ttaatccgac aagtttattg tttttatcta cgatgggcag tttttcaatg cgatattcat
     1861 gcaagatatc ctccgcctgt ttcaaggtag tcccttcgtg tgccgttatc agccggtctt
     1921 tagtcatcag gtcttttacc agcatgtttt tgtctttgca aaagcgcaaa tcgcggttgg
     1981 taaggatgcc cgccaaatac ccgttttcgt ctgtaaccgg aatcccgcct attttgtttt
     2041 cagccatcag aataaaagca gccgccactt tctgttccgg atttaaggta atcgggtcag
     2101 taattaagcc actttcagag cgtttaactt tgcgtacttc attggcttgt ttctcaattg
     2161 acatattttt gtgtaaaacg cctataccac cttctcttgc caatgcaata gctaaattag
     2221 cttcagtaac cgtatccata gccgacgata aaatgggaat attggcactg atattacgtg
     2281 ttacctgggt tttcaggttg gtttcgcgag gtaaaacttg tgagtaagcc ggaagtaaaa
     2341 ggacatcatc aaatgtcaaa ccttcaccgg caaatttgtg ttgcggggat aaatgaaagt
     2401 tgctttccat gtgcaaagat aggaaagatt ttttaaccaa ttaaggaatt attataacag
     2461 tctgctcaaa ttatcaaact gttacagcca atgcttttcg ggatagctga tacccttttt
     2521 ccaaatccga cattgatgta aatagtgcat cttcttttat ccattccgct tcaagatgtt
     2581 gttgggtagt atggaaattt tcccattgat gaataaaatc gtttctgaat ttccggtttc
     2641 tgatttccca tttccaccag tagctatcat ctgcatgtgc gccgggctgt tgaaattgct
     2701 cttcattcag caaggtgtct ccaaaataaa agggcttata taagctgata catgggtttg
     2761 aactaccggt aaaccatacc gtatgcggca gattattttt acgcaatacc gccaccatac
     2821 tccctgttgt ttggtgtggg caaaacaagc cgttattgtg catacaaatg gactttgtgc
     2881 ctgctctttt aggactgaaa ttgtcgggtt gctcatgtgt gcgcaagatg gaaaaagcag
     2941 cggaaacatc aaactgacgg aatatgcccg acttatgttc gctcattttt ctgcggtgtt
     3001 cgcaggccgc taaacgtggc atcaaaaact gagaaaacgc ttttt
//
LOCUS       DAOQII010000288         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_21784_length_3029_cov_4.205447, whole genome shotgun sequence.
ACCESSION   DAOQII010000288 DAOQII010000000
VERSION     DAOQII010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21784_length_3029_cov_4.205447"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PLF48_11040"
     CDS             <1..1104
                     /locus_tag="PLF48_11040"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNF49909.1"
                     /translation="YMDPHNNQELVDFNIQDYWKDVDLYVGGSEHAVGHLLYSRFWHK
                     FLKDIGKVSTEEPFKKMVNQGMIQGRSLLTKENQIKNIPAKLHIPVMLAPNDRITKAN
                     FAELIKADNRFEAIDIENDISWEEIDGQYFISLDVQVEKMSKRYLNVVNPDDMVLEYG
                     ADVFRMYEMFLGPIDTAKPWDTNGISGVANFLRKFWRLFFDDMGNKKYVDADASADEL
                     KILHKCIKKINTDIENLSLNTCISTFMICVNDLGKQKAVSASILTTLTKLIAPFAPHT
                     AEVLWTNLGMEGFVINQPFPKHDEKFLIESTFTYPVQINGKHRANIEISLDADENQVK
                     EIILADEQILKYMEDKQLKKLILVKGRIINIVV"
     gene            1409..1747
                     /locus_tag="PLF48_11045"
     CDS             1409..1747
                     /locus_tag="PLF48_11045"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNF49910.1"
                     /translation="MEKRFKSLKEFYPFYLTEHSDPTCRMLHYIGTTLVIAMFVYALI
                     QPSAWKIVAIPFLGYSFAWIGHFVFEKNKPATFQYPLWSLASDFKMFFDFLTGKVDKK
                     LAEAKLQFPK"
     gene            complement(1969..>3029)
                     /locus_tag="PLF48_11050"
     CDS             complement(1969..>3029)
                     /locus_tag="PLF48_11050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNF49911.1"
                     /translation="TQIAFMGGMIFSEKFRGMADQAMQGMQLLFLKFSRDDESQSDVL
                     GVKYSSAIGYDANQMADFFQTLQRYSEKEGARVPEFLSTHPDPGNRYSTVKQLAKDYQ
                     TTNNLTKLNINRDQYLRMIDGLVYGEDPRQGYLDKTNYVFYQPEMRFEYPIPNGWQYQ
                     NSPMAVQAANASGDAAIILTMSDKSTFDAAASEILQAYGLKNITTPITTKINGLDAYT
                     FTAEQVIDQQTIQQNGGKQPTDAEIMQVLFTFYKYDNKIYVMQGMSSKKDFPAQKSNF
                     LYSMNGFKPLTDPKRINVIPERIKIVSVANDATLKDIMVLYKIPADRYEEVSILNGMQ
                     QSTLVKKGMLVKMLEKKA"
BASE COUNT          993 a          483 c          551 g         1002 t
ORIGIN      
        1 tatatggatc cccataataa tcaagaactt gttgatttta acatacaaga ttactggaaa
       61 gatgttgatt tgtatgttgg tgggagtgag catgccgtag gccatttact ttattctcgt
      121 ttttggcata aatttttaaa agatatcgga aaagtaagta cggaagaacc attcaaaaaa
      181 atggtcaatc aaggaatgat acaaggaaga agtttgttga caaaagaaaa tcagataaaa
      241 aatattccgg ctaagttgca tattccggtt atgctggctc ctaatgacag aattactaaa
      301 gctaattttg cagagctcat caaagcagat aatcgttttg aagcaataga tattgaaaat
      361 gacatcagtt gggaagaaat agacggtcag tattttattt ccttagatgt gcaagttgaa
      421 aaaatgtcta aacgttattt aaatgtagtg aatccggatg atatggtatt ggaatacgga
      481 gcagatgttt tcagaatgta tgagatgttt ttaggaccga ttgatacggc aaaaccttgg
      541 gacaccaatg gcatttctgg agtagctaat ttcttgcgca agttttggcg attgtttttt
      601 gatgatatgg gaaataagaa atatgtggat gcagatgctt cagcagatga attaaaaatt
      661 ctgcataaat gcattaaaaa aataaataca gatatcgaaa atctgagttt gaatacttgt
      721 atttctacct ttatgatttg tgttaatgat ttgggaaaac aaaaagccgt ttccgcatca
      781 atattaacaa cattgaccaa acttattgct ccgtttgcac cacatacggc tgaagtactt
      841 tggacaaatt taggaatgga gggatttgtc atcaatcagc ctttcccaaa acatgatgag
      901 aaatttttga tagaaagtac cttcacttac ccggtacaaa ttaatggcaa gcaccgcgct
      961 aatattgaaa tttccttgga tgcagacgaa aatcaagtga aagaaatcat tttggctgac
     1021 gaacaaattt tgaaatacat ggaagataag cagctgaaaa aactgatatt agtaaaaggc
     1081 agaattatta atatagtggt gtaacctctt gtgcggatag ttttatagtt tttaccaaat
     1141 agaggcaaag attttgttta gcattaacaa tgtttatcat acaaaaatta aggtaagaag
     1201 agaaaaatat aggaatcgcc aaaggcgata ctatgttttc ttcttgatta ctgtatttct
     1261 ttgcagtaaa taactattta tgattaaaaa tgctatgctt ctatgtggtt taaaaagtgc
     1321 aattaatttg gaataataag atttttatca gctgaaaata ataacacagc gacttctcaa
     1381 atttttataa atttgacaaa gtctaattat ggaaaagcgt ttcaaatcat taaaggaatt
     1441 ttatcccttt tatcttaccg agcattcgga tcccacttgt cgcatgttgc attatatagg
     1501 cactacattg gtgattgcca tgtttgtata tgctctgata cagccttcag cttggaaaat
     1561 agtagccatt ccattcttag gttacagttt tgcctggata ggtcattttg tatttgaaaa
     1621 aaacaaaccg gcaacatttc aatatccttt atggagttta gcatccgatt tcaaaatgtt
     1681 ttttgatttt cttacaggta aagtggacaa aaaattagct gaagccaaat tacaattccc
     1741 gaaatagaaa ctgttggcaa aaatcaccgt catatcgaac gaagtgagat atccgtttca
     1801 gtattaggct aaagtacaag tcatatcaaa aatagtgaga tatccgtttt agaagtatgc
     1861 agtcttttaa ttggcaaatt caattacagc acgcttcaaa attattaaac attcatcact
     1921 aaacattcaa catttctaag catagctttg aatagtcagc tataaaactt atgctttttt
     1981 ctccagcatt ttcaccagca tacctttttt caccaaagta gattgttgca tgccgtttaa
     2041 aatagatact tcttcatatc ggtcagcagg aattttgtaa agtaccataa tatcttttaa
     2101 tgtagcatca ttagctactg atacaatttt aatacgctca ggtataacat taattctttt
     2161 tggatcagtt aaaggtttaa aaccattcat gctgtataag aaattagact tttgagcagg
     2221 aaagtccttt tttgagctca ttccttgcat aacatatatt ttgttgtcat acttgtaaaa
     2281 agtaaacaat acctgcatga tttctgcatc cgtcggttgc ttaccaccat tttgctgtat
     2341 ggtttgctgg tcaattactt gttcagctgt aaatgtataa gcatctaagc cattgatttt
     2401 tgttgtaatc ggtgtagtga tatttttaag tccgtatgct tgcagaattt ccgaagcagc
     2461 agcatcaaat gttgatttat ctgacatagt caaaataatt gccgcatcgc cactggcatt
     2521 tgccgcttgt acagccatag gtgaattttg atattgccag ccattgggaa ttgggtattc
     2581 aaatcgcatt tcaggttgat aaaatacata gttggtttta tccaaatagc cctggcgcgg
     2641 atcttctcca tataccaatc cgtcaatcat gcgaagatat tgatctctgt tgatattgag
     2701 cttggttaaa ttgtttgtgg tttgataatc ttttgcgagt tgttttaccg tgctgtaacg
     2761 attgcccgga tccggatgtg tagataaaaa ctcaggaaca cgtgctcctt ctttttcaga
     2821 atatcgttgt aatgtttgaa agaaatctgc catttgattg gcatcatatc cgatagcaga
     2881 agagtatttt acacctaata catcagattg agattcatca tctctcgaaa attttaaaaa
     2941 taataattgc atgccctgca tagcttggtc ggccatgcct ctgaattttt cagaaaaaat
     3001 catcccaccc ataaaggcaa tttgagtaa
//
LOCUS       DAOQII010000289         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_21963_length_3014_cov_4.948293, whole genome shotgun sequence.
ACCESSION   DAOQII010000289 DAOQII010000000
VERSION     DAOQII010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21963_length_3014_cov_4.948293"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLF48_11055"
     CDS             complement(<1..354)
                     /locus_tag="PLF48_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNF49912.1"
                     /translation="MKRPRTLAEPFKIKMVEPLKITTEAYRKTQLEKAFNNPFLLKSE
                     DVFVDLLTDSGTGAMSEQQWSAMMLGDESYAGASSWEKLETEVKDLTQMPFVIPTHQG
                     RAAERILYGILGGKDK"
     gene            complement(727..1296)
                     /locus_tag="PLF48_11060"
     CDS             complement(727..1296)
                     /locus_tag="PLF48_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49913.1"
                     /translation="MKRIIASIALFILLLAATPSFAIEKGDFNIYTTLNLGHHSYIPT
                     YTTKGYGFVPGVTVNMDYAASPYFGIGGWFTFSGRKYNAGSLYAIDYKYRNFGVGVRG
                     VFHLYQLISEKGNTKLDAEKFDVYIPLAVGGGFRLKDKNLAGDKFGAGAIVGAGIGMS
                     YYFVEHIGGNLEAGYLEGSYAKLGLVFKF"
     gene            complement(1345..>3014)
                     /locus_tag="PLF48_11065"
     CDS             complement(1345..>3014)
                     /locus_tag="PLF48_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49914.1"
                     /translation="AIDEAFSRHAAENIGAVIITGDGIYNKGNSPLYNAKALQVPVYT
                     VALGDTTLKKDAYIKSVRCPDVVYLGDQFSISVQIEAHHLKGQQTSLQIIDFNGRIIA
                     SKVISITDDNYIDQIEAVGNAVKQGLLPFKVMLKPMPGEMTAENNYETAYVEVIDGRQ
                     RILLLYDAPHPDIKALRYAIEQNKNYQLEATDIKMYSGNIKETDMVILHGLPSLGASS
                     KLGLLSEIVNAQVPVFFILSANTNLAQLNTLQKVLQISGSAQNGNEVFPVYNSSFSKF
                     TVNEQMIKTLQNLPPLLAPFGKYTLSATADVFLKQQIGNIPTNNPLLFFNEINGKKSG
                     FLCGEGIWRWRMHEYLQTKSYAATDELVNKCIQYLTVKNDKRKFKIKPLKNVYNANEQ
                     VEMEAELYNESYELVNDEDVQCVVKGDNGKEFNYALDKTLNAYYLNAGILPVGNYTAL
                     AKTTYKGKPLTASCSFSVRAINVEIINTQANHQLLQNLSAQSGGKMYYPQNMSAIFDD
                     LKNQAKMKTILFDTFNTRPLIDFKWLFVLALLILALEWFLRKFNGII"
BASE COUNT          927 a          593 c          502 g          992 t
ORIGIN      
        1 tttgtctttt ccacccagta tgccatacaa aattctttct gctgccctac cctgatgtgt
       61 aggaataaca aaaggcattt gcgttaaatc tttaacttct gtttctaact tttcccaact
      121 gcttgctccg gcataactct catcgcccaa catcatggca gaccattgtt gttcgctcat
      181 agctcctgtt ccgctgtctg tcagcaaatc gacaaagaca tcttcacttt tgagtaaaaa
      241 ggggttgtta aatgctttct ctaattgagt tttgcgataa gcttctgtag taattttcaa
      301 tggttctacc attttaatct taaatggttc tgccaaggtt cgaggtcttt tcatgcaaaa
      361 atttagacaa caaatttaag gtaaaaagcg gtaagaagtt agtgattgta agattgtaca
      421 aataagtgga tggattactg atgaaagctt atcagtaaaa aataataata taacccattg
      481 tgcgatttaa accaaataga atcatagtat ttctaatcat aaatcgttgc ttgctacaaa
      541 ccaatataga ttacaagcaa aaacctaata tcgcctttga cgattcttat atttttctat
      601 tcttacttaa ttttgtaagg aaaacattgt taatcctaaa aaatctatac cactatgtgg
      661 taaaaattat aaaacaaaac acacaaaaag ttaaaatact ttacactaaa gagagattgg
      721 ctggtactag aacttaaata ccaaacccag cttggcatat gatccttcta aatacccggc
      781 ttctaggtta ccaccgatat gttcaacgaa ataataagac ataccgattc ctgctcctac
      841 tatagcacct gcaccaaatt tatctcctgc aaggttttta tcttttaatc tgaaaccacc
      901 accaacagca agaggaatat aaacatcaaa tttttcagca tctaatttag tgtttccttt
      961 ctctgagatc aactgataca aatgaaaaac gccacgtaca cctacaccga aatttctgta
     1021 tttatagtcg atggcgtata atgaacctgc attatatttt cttcccgaaa aagtaaacca
     1081 accgccaatt ccaaaatatg gtgaagcagc ataatccata ttgactgtta caccaggaac
     1141 aaaaccgtag ccttttgtag tatatgtggg aatatagcta tggtgtccca aattaagtgt
     1201 cgtataaata ttgaaatctc ctttttcgat agcaaaactt ggtgtagctg ctaacaataa
     1261 aataaataat gcaattgagg caataattct tttcataaag ctgatatttt actgtacaaa
     1321 tgtaacgtat tttttttaga gcaactaaat gatgccatta aactttcgta aaaaccactc
     1381 taatgccaat atgagtaatg ccagtacaaa aagccattta aagtcgataa gtggacgggt
     1441 attaaaagta tcaaataaga tggttttcat tttagcttga tttttcaagt catcaaatat
     1501 agccgacata ttttgtggat agtacatttt acctccgctt tgtgccgaca agttctgcaa
     1561 caactggtga ttggcttgcg tgttgatgat ttctacattg atagcccgta cggagaaact
     1621 gcatgaagca gttagcggtt tgcctttata tgtagttttt gccaatgctg tatagttgcc
     1681 cacgggcaaa attcccgcat tcagataata ggcattcagt gttttatcca gcgcatagtt
     1741 aaactctttt ccattatcgc cttttacgac acattgtaca tcttcgtcat tcaccagctc
     1801 ataactttca ttatataatt cagcttccat ttccacctgc tcattagcat tatatacatt
     1861 tttaagaggt ttaatcttaa atttccgttt gtcatttttc accgtcaaat actgaatgca
     1921 tttattgacc aactcatctg ttgcagcata gcttttggtt tgcagatatt catgcatacg
     1981 ccaacgccag atgccttcgc cacataaaaa tccggatttc ttgccattaa tctcattaaa
     2041 aaacaagagc gggttattgg tcggaatgtt gccgatttgt tgtttcagga aaacatctgc
     2101 tgtggcagaa agggtgtatt ttccaaatgg tgccaataat ggtggtaaat tctgcagtgt
     2161 ttttatcatt tgctcattta ctgtaaattt ggaaaacgag gaattataaa ccggaaaaac
     2221 ttcattgcca ttttgggccg aaccacttat ttgcaacact ttttgcagtg tgtttaattg
     2281 tgccagattc gtatttgccg ataaaataaa aaagacaggc acttgagcat tcactatttc
     2341 ggaaagcaaa ccgagttttg aagatgctcc taaagacgga agcccgtgca aaatgaccat
     2401 atcggtctct ttaatattac ccgagtacat tttgatatcc gttgcttcca gttggtaatt
     2461 tttattttgt tctatggcat agcgcaacgc tttgatatcg gggtgcgggg catcatacaa
     2521 caacaatatt ctttgtctgc catctatcac ttctacgtat gctgtttcat aattattttc
     2581 tgctgtcatt tctcccggca ttggtttgag cataaccttg aaaggcaata aaccctgctt
     2641 gacagcattg cctacggctt ctatttggtc gatataatta tcatctgtaa tggaaatcac
     2701 ttttgatgcg attattcttc cattaaaatc tatgatttgt aagctggttt gctgaccttt
     2761 taggtgatgc gcttcaatct gaacagagat agaaaactgg tcgcccaaat aaaccacatc
     2821 cggacaacgt actgatttga tataagcatc tttctttaat gtggtatcgc ctaacgcaac
     2881 tgtataaacc ggaacttgta atgctttagc attatataaa ggcgaatttc ctttgttgta
     2941 aatgccatct ccggtaataa tcacagcgcc aatattttct gcagcatgtc ttgaaaatgc
     3001 ttcatcaata gcag
//
LOCUS       DAOQII010000290         2994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_22206_length_2994_cov_4.173528, whole genome shotgun sequence.
ACCESSION   DAOQII010000290 DAOQII010000000
VERSION     DAOQII010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22206_length_2994_cov_4.173528"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="PLF48_11070"
     CDS             <1..1226
                     /locus_tag="PLF48_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49915.1"
                     /translation="NEWLSEIEILYHKGNFKSCVKHVYKAQHLARSADEFLYLLKLLH
                     YEKLVNAHVSYNNRTDEVIDKETKALYKLIENVMQFDDLFHNATQLRITIAKSRASAD
                     INKLEKLLAQKLLSKPSYALCTASKIKYYQIHAINNFIQRNTKNELANNEAVIQLMDS
                     KPYFKRLHLLEYVNTFARILSITKNTDESQFYLALDRIRSISISTDELHYTRTTAQIF
                     IFSYMTEFSFYLQNKYFNKIISIIPEIESGMKKYKEILIASYHVTIIYMLAYYYFCIG
                     DFDNAGNYINRLLNEFEEKNRPDIYNFAKLLNLFIHLEKGNFTYIKYKQANVRYYFKK
                     QSALHATENAILDFFSKEKNYTINFKQSLLILSAELSEMQQDKMERFTLNYFDVNSWI
                     NAKLTGKKTIADLFA"
     gene            complement(1235..2599)
                     /locus_tag="PLF48_11075"
     CDS             complement(1235..2599)
                     /locus_tag="PLF48_11075"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF49916.1"
                     /translation="MQKHYTWKAIKLLIVIFISWNAISCEKDNDVSSTDDTLNSLLIK
                     ASGGRGSAYFKLPSGANLAEFPQDPRNRLTPEKVALGKILFHETCIGSNPKNTQGMMT
                     YSCASCHHVEAGFQAGIAQGIGEGGIGFGTNGEQRKINPSYPVDLIDVQPIRSPTVLN
                     TAYQELMLWNGQFGAVGKNAYVQYAWTPGTPKETNLLGFQGLETQAIAGQNVHRLKID
                     KTFLGNITSIKYLFDQAFADIPETGRMTPINAGLAIAAYERTLVSNEAPFQKWLNGNN
                     NAMTETQKQGALLFFGKAGCVQCHTGPALNSMEFYALGMDDLLTGNYGAINAGADKAE
                     HKGRGGFTGKAEDMYKFKVPQLYNLKGVKFLGHGAQFESVKEVLDYKNKAIKSNAIVP
                     DTQLASAFKPLNLTDDELEKLRVFIEDGLYDANIKRYAPLTIPSGFCFPDNDTQSRID
                     KGCQ"
     gene            complement(2736..>2994)
                     /locus_tag="PLF48_11080"
     CDS             complement(2736..>2994)
                     /locus_tag="PLF48_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49917.1"
                     /translation="RTLNSLEEKKAIIRKPAEEDQRKVLVVLTEEGKRLRKIALRTVF
                     NLEKKITENLSPDELAIFFKVVDSISGTIDTFRTELAKQSS"
BASE COUNT          923 a          547 c          530 g          994 t
ORIGIN      
        1 taaatgaatg gctttctgaa atagaaattt tgtatcataa aggtaatttc aaatcatgtg
       61 ttaaacatgt ttacaaggct caacatttag ccagatcggc agacgagttt ttatatttat
      121 taaaactgtt gcactacgaa aagttggtaa atgcccatgt cagctataat aaccgtactg
      181 atgaggtaat tgacaaggaa acaaaggccc tctataaact cattgaaaat gtaatgcagt
      241 ttgatgattt atttcataat gctactcaat tgagaattac tattgcaaaa tcaagagcga
      301 gtgctgatat aaataaattg gaaaaactgc tggcacaaaa attattgagc aaaccgtctt
      361 atgctctttg tacggcaagc aaaataaaat attatcaaat ccatgcaata aataatttta
      421 ttcagcgaaa tactaaaaat gaattggcaa ataatgaagc agtaattcaa cttatggaca
      481 gcaagccata ttttaaaagg cttcatctgc ttgagtacgt caataccttc gcccgcatat
      541 tgtccattac taaaaataca gatgaatcgc agttttatct ggcattagac agaatacgca
      601 gcatttcaat ttcaacggat gaattacatt atacacgtac aacggcacag atttttatct
      661 tcagctacat gacagagttt tctttttact tgcagaataa atattttaat aaaataattt
      721 ctattattcc agaaattgaa agcggtatga aaaaatataa agaaattctt attgcttctt
      781 atcatgtgac aataatttac atgctggctt attactattt ctgtattggt gattttgata
      841 atgccggaaa ttatatcaac cgattactga atgaatttga agaaaaaaat cgtcctgata
      901 tatacaattt tgccaagttg ctgaatttgt ttattcattt agaaaaagga aattttacct
      961 atatcaagta taaacaagcg aatgtgcgat attattttaa aaagcaaagt gcgttacatg
     1021 ctactgaaaa tgcaatactg gatttttttt ccaaagaaaa aaattatacg ataaatttta
     1081 agcaatcttt gcttattctt tcagctgaat tgtctgaaat gcaacaagat aaaatggaga
     1141 gatttacgct caattatttt gatgtaaaca gttggataaa tgctaaactg acgggcaaga
     1201 aaacgattgc agatttattt gcttaaaaat ttgcttactg gcagcctttg tctattctgg
     1261 attgagtgtc attatccgga aagcaaaatc ctgaaggtat ggtaagtggc gcatatcgct
     1321 tgatatttgc atcatataag ccatcttcaa taaatacgcg caatttttct agctcatcgt
     1381 cagtcaggtt taatggctta aatgcactgg ccaactgtgt atccggaact atcgcatttg
     1441 atttaatagc tttgttttta tagtccagta cttcttttac cgattcaaat tgcgcaccat
     1501 gtcccaaaaa tttaacgccc ttcaaattat acaattgcgg tactttaaat ttatacatgt
     1561 cttcggcttt gccggtaaat ccacctcgtc ccttatgttc tgctttgtct gctcctgcat
     1621 taatggcacc ataatttccg gtcagcaaat catccatgcc gagcgcatag aattccatcg
     1681 aattgagtgc aggtccagtg tggcattgta cgcatccggc tttaccaaaa aagagcagtg
     1741 caccttgttt ttgtgtttct gtcattgcgt tattgtttcc attcagccat ttttgaaaag
     1801 gagcttcatt gcttaccagc gttctttcgt atgcagcaat agccaatcct gcattgatag
     1861 gagtcatcct tcctgtttcg ggaatgtcgg caaatgcctg gtcaaacaaa tattttatag
     1921 atgtaatatt tcctagaaat gttttgtcta tttttagtcg atgcacattt tgcccggcta
     1981 tggcttgtgt ttccagtcct tgaaatccta ataaattagt ttcctttgga gttcccggtg
     2041 tccaggcata ttgcacataa gcatttttgc ctacagcacc aaattgtccg ttccacaaca
     2101 ttaattcttg atatgctgta tttaataccg ttggcgaacg aatgggttgc acatcaataa
     2161 gatcaacagg ataagacgga ttaatttttc tttgttcacc gttagtgcca aatccgattc
     2221 ctccctcacc gataccctgt gcaataccgg cttgaaatcc ggcttccaca tgatggcaac
     2281 tggcacagga ataggtcatc attccttgtg tgtttttagg gtttgatcct atacaagttt
     2341 cgtgaaacaa aattttgcca agagctactt tttcaggggt gagcctgttt cgcgggtctt
     2401 gtggaaattc agccaaatta gcaccggaag gcagcttaaa ataagcacta ccgcgaccac
     2461 ctgaggcttt tatcaataag ctgttcagtg tgtcatctgt agaagaaaca tcgttgtctt
     2521 tttcacaact gatggcattc caggaaataa aaattacaat aagtaatttt atagctttcc
     2581 aagtgtaatg tttttgcatg tataaaaaat aatttcacgt gactaatcga actatagact
     2641 gaataaatga gaatttagtt gcgaaagcta aggtaaaata tatttatctt aacatgaatg
     2701 gcaaatatta tttttagcaa aatttttgca attcactaac ttgactgttt ggcaagttct
     2761 gtgcgaaagg tatctattgt gcccgatatg ctgtctacta ctttaaaaaa tatggccagt
     2821 tcatccggac ttaagttttc tgtgattttc ttttccagat taaaaacagt tcgcaaggca
     2881 attttgcgca atcgcttgcc ctcttccgta agcactacca aaactttgcg ttggtcttct
     2941 tcggccggct tgcggatgat tgctttcttt tcttccagac tgtttagggt acgg
//
LOCUS       DAOQII010000291         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_22509_length_2970_cov_5.039794, whole genome shotgun sequence.
ACCESSION   DAOQII010000291 DAOQII010000000
VERSION     DAOQII010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22509_length_2970_cov_5.039794"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLF48_11085"
     CDS             <1..665
                     /locus_tag="PLF48_11085"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNF49918.1"
                     /translation="TDSLLSVNSTGIIAEFKRKSPSKGIINNTAQVEAVTKAYAENGA
                     SGVSVLTDELFFGGSKNDFLKTRDLIEIPLLRKDFMIDEYQFIEAKAWGADVVLLIAA
                     ILSPRQVRDFTNLSHDLSMQVLLELHDETELDHVYHEVDMVGINNRNLKTFEVDIEQS
                     IRMANYLDKDFTKIAESGISSIETLKYFKNNGFQGFLMGEYFMKNMNPAHAFKDFISF
                     LY"
     gene            760..1254
                     /locus_tag="PLF48_11090"
     CDS             760..1254
                     /locus_tag="PLF48_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49919.1"
                     /translation="MLKLYTLCLFSLFFYSSSSLRAQTQALDSLWNNQFIKKYSEAII
                     KHDSTMYFNLLISQVEIDSIVASLKTENPNCITDIESTMNVSLLKKQFQNVLSFFRNV
                     RSIKFGKGYYINGCADIKGIKLNISGICGPQKVERTRTLSFIQMNNSRYKLIFDVAVT
                     NSNQ"
     gene            1271..>2970
                     /locus_tag="PLF48_11095"
     CDS             1271..>2970
                     /locus_tag="PLF48_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49920.1"
                     /translation="MRNSFYNIALCLVILFLAEEHSQAQNYNDGRIRLRVWIHKVWSN
                     SNCGEIGNQEYRFKGIRCRIPDGAGGFAYSNSYNIAFSGDNNRYYDASQFTPTPAALS
                     NDGVASGYKIIDNSYPATLVPNQFDVIMSEAFEEDCESSGLLDCGQGTYNIYDQCCCL
                     FGICASSDEYLGSGQWTGVLFRGAEPGKINITQPFVINPQDEHSYSVAFAYQWDWIDP
                     LLPTCSSPKYSDGSNASPIALTVEFDKVFTDVDWDGGLGCGTGVAGDEDLRLKFRTKD
                     NITGAMPAWGASSIHINQNKPKWNDPANTTIFSKSYLTADLNMESVDIEYDLWEEDGF
                     TQTVFGNTYVPCGDANWDNNYNPSCSVTVLGITVSWQGDDDHATGTFNLNWRNSPPNT
                     DNFVEIPVRLSGSQYSSWIVRLKYRWNIANPVVNMPDADYLKCIGTALTLTPASTTNA
                     TYYQWQVADVTTSGAPICPATANWTNISGAICSSYTVPQTPGTRIYRLAVYNRNGAGS
                     KTSSGGRYAVSYSGCQRVTYLPYAPPIASTACGGSVPSGTYTFSATAAPSILAIGSAV
                     T"
BASE COUNT          929 a          499 c          597 g          945 t
ORIGIN      
        1 taacagacag cctgctctct gtaaattcta ccggaattat tgcggagttt aaacgaaaat
       61 cgccttcaaa aggaattata aataatacgg ctcaagttga agcagtaaca aaagcgtatg
      121 cagaaaatgg agcatccggc gtttccgttt tgaccgatga attgttcttt ggtggaagta
      181 aaaatgattt tctaaaaact cgagatctta tagaaattcc cttattgcga aaagatttca
      241 tgatagatga atatcagttt atagaagcca aagcatgggg tgccgatgtt gttttgttga
      301 ttgcagctat actttcccca cgtcaggttc gtgattttac taacctatca catgatttga
      361 gcatgcaggt tttgctggaa ttgcatgacg aaactgaact tgatcatgta tatcatgaag
      421 tagatatggt tggaattaac aaccgtaatt tgaagacatt tgaagtagac atcgaacagt
      481 cgatacgaat ggcaaactac ttggataaag attttaccaa aattgctgaa agtggcattt
      541 caagcataga aacattgaaa tattttaaaa ataacggttt ccaaggcttc ctgatgggag
      601 aatattttat gaaaaatatg aatcctgccc atgcatttaa agattttatc agttttctat
      661 attaagtatt tatacaaaat atagattttc aattgtttat aaataaaatt tctgttgcac
      721 aagttatagt tttcatgtaa atttattgta aactaatgca tgttgaaact ttatacactt
      781 tgtttgttta gcttgttttt ctatagttct agttctctta gggcacaaac acaagctctt
      841 gatagtcttt ggaataatca atttattaaa aaatattctg aggcaattat caagcatgat
      901 tcaacgatgt acttcaatct attaatttct caagttgaaa ttgactcaat tgttgcaagt
      961 ttgaaaacag aaaacccaaa ttgtattacc gatatagaga gtactatgaa cgtgagcctg
     1021 ttaaaaaagc agtttcaaaa tgtactttca ttttttagaa atgtacggtc aataaaattc
     1081 ggaaaaggtt actatatcaa tggctgtgcc gatattaaag gtataaaatt aaatatttca
     1141 gggatatgcg gacctcagaa agtagaacga acgcgaacac tttctttcat tcagatgaat
     1201 aatagccggt acaagttgat tttcgatgtt gcggttacga attcaaatca ataaataaaa
     1261 aacataacct atgagaaata gtttttacaa tatagcacta tgcttagtaa ttcttttctt
     1321 agcagaggaa cacagtcaag ctcaaaatta taatgacggt agaattcggt tgcgtgtttg
     1381 gattcacaag gtttggtcaa attctaattg tggtgaaata ggtaatcaag aatatcgctt
     1441 taaaggaatc cgttgcagaa ttccggacgg tgcaggtggg tttgcttatt ctaactctta
     1501 taatattgct tttagtggag ataataatcg atattatgat gccagtcagt ttacacctac
     1561 cccggctgca ttatcaaatg acggggtggc aagcggatac aaaattatag ataattcata
     1621 tccggcaaca ttagtcccca accagtttga tgtaatcatg tctgaagcat ttgaagaaga
     1681 ttgtgagagt agcggattac tggattgcgg acaaggtact tacaatattt atgaccagtg
     1741 ttgttgtttg tttggaattt gtgcaagttc agatgaatac ctcggctcgg gtcagtggac
     1801 tggcgttttg tttagaggag ctgaacccgg taaaattaat atcacgcaac cttttgtaat
     1861 aaatcctcaa gatgaacatt cttattctgt agcttttgcc tatcagtggg actggataga
     1921 tccacttttg ccgacttgtt catcaccaaa atattcggat ggctctaacg caagtccgat
     1981 agcattaaca gtagagtttg ataaagtatt tactgatgtt gattgggatg gtggtcttgg
     2041 ctgtggtaca ggtgtggcag gcgatgaaga tttacgtcta aaattcagaa ctaaagataa
     2101 tatcacaggg gctatgcctg cttggggtgc atcttccatt catattaatc agaataaacc
     2161 taaatggaat gatcctgcaa atactaccat tttttcaaaa tcatatctga cagcagacct
     2221 taacatggaa tctgtagata tagaatatga tttatgggag gaagatggtt tcacacagac
     2281 tgtatttggg aatacttatg tgccttgtgg tgatgctaat tgggacaata attataatcc
     2341 aagttgttcg gttactgttt taggtattac tgtgtcgtgg caaggcgatg atgatcatgc
     2401 aacaggcaca ttcaatttaa actggcgaaa tagccctccg aatacagaca actttgtaga
     2461 aattcctgtt cgtttatccg gtagccaata ctcaagttgg atagttcggc tcaaatatag
     2521 atggaatatt gctaatccgg ttgtcaacat gcctgatgct gattatttga aatgtattgg
     2581 tactgctctt acattaactc ccgcatctac gacgaatgct acttattacc aatggcaggt
     2641 ggcagatgta actacttcag gtgctccaat atgtccggca acagcaaact ggacaaatat
     2701 ttcaggtgcg atttgttcat catataccgt tccacaaaca ccgggcacaa gaatttacag
     2761 gctcgctgtt tataatagaa atggtgctgg aagtaaaact tcttccggtg ggcgatatgc
     2821 cgtttcttat tccggatgtc aacgcgtaac atacctgcct tacgcacctc caattgcaag
     2881 tacagcttgt ggtggttcag taccatcagg cacctatacg ttttcagcta ctgctgctcc
     2941 tagtatttta gcaattggtt cagctgtaac
//
LOCUS       DAOQII010000292         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_22652_length_2960_cov_4.965232, whole genome shotgun sequence.
ACCESSION   DAOQII010000292 DAOQII010000000
VERSION     DAOQII010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22652_length_2960_cov_4.965232"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLF48_11100"
     CDS             complement(<1..698)
                     /locus_tag="PLF48_11100"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF49921.1"
                     /translation="MSAFTSDTEGINLLSSKYNELKSEIAKVIVGQDEVVKYVLLSIF
                     CNSHSLLVGVPGLAKTLLVKTVADALDLDFKRIQFTPDLMPSDIIGAEILNENRQFQF
                     NRGPIFANIVLADEINRTPPKTQSALLEAMQERQVTAAGQEHLLDLPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIILDYPTYAEELNVVKLTTSDHANAVRKVLTGKQIIDFQH
                     LIRRMPIADNTVEYA"
     gene            complement(703..2040)
                     /locus_tag="PLF48_11105"
     CDS             complement(703..2040)
                     /locus_tag="PLF48_11105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF49922.1"
                     /translation="MQKTTFLFFILLLLPFSGFCEDKLLDKIDAIIGDKIILRSDIEN
                     QLDLLNMRGKDVEQNRCTLMQQLILTRMLANQAIRDSLPISDEEVEDELNRKVNYFIS
                     MAGSQEAFEAYYKKSVQQIKDDYREDIRENMLSSRMRGKITENIKITPAEVKKYFEEM
                     SKDSLPYFNATMEVKQIVMKPKVSDLQKELALKKTQELLERLKKGESFDLLASLYSED
                     IASAQQGGLLDWVPRGTFVKEFEAAAFKLKDGEISDIVETPFGYHIIQMVERRGDMIQ
                     VKHILIRPKSTSADIEYARLKLDSVRNDILAEKITFEKAVKEYSTDDASKNVGGFILN
                     NENGSTLLDVTKMDNELYLLVDTLKENAITFASLFNDNDGTPAYRIVKLISKTNPHEA
                     SLQVDYDKIQNAARASKEEEEILKWFDKYLKKTYIMVDDEYKSCDVLQSILKN"
     gene            complement(2246..>2960)
                     /locus_tag="PLF48_11110"
     CDS             complement(2246..>2960)
                     /locus_tag="PLF48_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HNF49923.1"
                     /translation="LRDMSKDTTPSVKNITIDVGALDKKEVEAMGIHVGCVITYEDEL
                     MKLNNRYYTGRALDNRIGGFMIAEIARLVKQAKKKLPFGLYICNSVQEEVGLRGAEMI
                     VRQINPNIAIVTDVCHDTTTPMIDKKDNGDFKCGRGPVITYGPAVHNILRELILDTAE
                     ASKIPFQRDAVSRSTGTDTDAFAYNGGGVPSALISLPLRYMHTTVESVAISDVEHVIQ
                     LIYDTLQNISPKMNLKYLK"
BASE COUNT          885 a          591 c          496 g          988 t
ORIGIN      
        1 gcatattcta ccgtattgtc agcgatgggc atacggcgta tcaagtgctg gaaatctatg
       61 atttgtttcc cggtcagtac tttacgaact gcatttgcat gatctgaagt ggttagtttt
      121 actacattca attcttcagc ataagtggga taatccaaaa tgatattgaa cataaagcgg
      181 tcgagttgcg cttcaggcaa tggataagta ccctcttgct caattggatt ttgagtagcc
      241 agtacgaaaa atggcaaatc taataagtgc tcctgccctg ctgcagttac ttgtctttcc
      301 tgcatagctt ccagtaaggc agattgtgtt ttgggaggtg tacggttaat ttcgtcagct
      361 aatacaatat tggcaaatat gggaccgcga ttaaattgaa attgtctgtt ttcattcaaa
      421 atttctgcac cgatgatgtc agatggcatt aaatcgggcg taaattgaat acgtttgaaa
      481 tccaaatcca atgcatctgc aactgttttc accaataatg ttttcgccaa tcccggcaca
      541 cctaccagta agctgtggct attgcaaaaa atagacaaca ggacatattt cactacttca
      601 tcctgaccaa ctattacttt agctatctcc gattttagtt cattatattt tgaagatagt
      661 aaatttatgc cttccgtatc gctagtgaat gctgacatat tgttagtttt ttagtattga
      721 ttgtaagaca tcgcaagact tatactcatc atccaccatg atgtacgttt ttttaagata
      781 tttatcaaac cattttaaaa tttcttcttc ctctttagaa gctcttgctg cattttgaat
      841 tttgtcgtag tcgacttgta aactggcttc gtgcggatta gttttagata ttaattttac
      901 gatacgataa gccggtgtgc catcattatc attaaataaa ctggcaaagg taattgcatt
      961 ttcttttaaa gtatcaacca aaagatataa ttcattatcc atcttagtca catcaagcaa
     1021 agtacttccg ttttcattat tgaggataaa tccgcccaca tttttggagg catcatcagt
     1081 ggaatattcc ttcacggctt tctcaaaagt aattttttct gccaaaatat cgttgcgaac
     1141 agaatccagt ttcaatcggg catactcgat atccgcagaa gttgatttag gtctaataag
     1201 tatatgttta acttgtatca tatcccctcg tctttccacc atttgaatta tatgataccc
     1261 gaaaggtgtt tccacaatat ctgatatttc accgtctttc agtttaaaag cagcagcttc
     1321 aaattctttc acaaacgtac cgcgcggcac ccagtctaat aaaccaccct gttgtgctga
     1381 tgcaatatct tctgagtaca aacttgccaa taaatcaaaa ctttctcctt ttttcagcct
     1441 ttccagcaat tcttgcgttt tcttcaatgc taattctttt tgtaaatcac ttactttagg
     1501 tttcatcact atttgtttga cttccatagt tgcattgaaa tatggcaaac tgtctttcga
     1561 catttcttca aaatattttt tcacttcagc aggtgtaatt tttatattct cagtgatttt
     1621 acctcgcata cgagatgaca acatattttc acgaatatct tcccgataat catcttttat
     1681 ctgctgtaca ctttttttgt aatacgcttc aaatgcttcc tgtgaacctg ccatactgat
     1741 aaaataatta accttgcgat tgagttcgtc ttctacttct tcgtcgctga taggcaaact
     1801 gtctctgatg gcctgattgg caagcatacg ggttagaatt agttgctgca taagggtgca
     1861 acggttttgt tccacatctt tacccctcat attgagcaaa tccaactgat tttctatatc
     1921 tgagcgcaag atgattttat ctccgatgat ggcatcaatt ttatccagaa gtttatcttc
     1981 acaaaaacct gaaaaaggca aaagcaacaa aatgaaaaac aaaaaagtag ttttttgcat
     2041 aagcaattaa aataatgtgc aaatgtaaga aaatgcatgt aaggaaagga taaaattttc
     2101 ctcaaaacag gattaaaatg aaagggttaa gtttttttaa cggaagtcgg tatttactaa
     2161 gaaaaaaaca agataaaagt tgactttcaa atcaagttga atactaaacg taatcattaa
     2221 gcaagaaaaa gtattaaaat tttgattact tcaaatattt caaattcatt ttaggagaaa
     2281 tattttgcaa ggtatcatat atcaactgaa taacatgttc tacatctgat atggcaacag
     2341 actccacagt ggtatgcata tagcgcaaag gcaatgaaat caaggcactt ggtacacctc
     2401 caccgttata ggcaaacgca tccgtatcgg tgccggtgct gcgacttacg gcatcgcgct
     2461 ggaatggaat tttactagct tcagcagtat ctaatatcaa ctctcgcaag atattatgaa
     2521 cagcaggacc atatgtaatc actggtccac gtccgcactt gaaatctcca ttatcctttt
     2581 tgtcaatcat aggtgtagtt gtgtcgtgac aaacatccgt aacaattgca atattcggat
     2641 tgatttgtcg aactatcatt tctgccccgc gcaagcctac ttcttcctga accgaattac
     2701 agatatataa accgaaagga agtttctttt tagcttgttt taccaatcgg gcaatttctg
     2761 caatcataaa tccgcctata cggttatcga gggcacgacc tgtatagtaa cgattattca
     2821 gtttcatcag ttcatcctca taagtaatta cacagcctac atgtatgccc atggcttcca
     2881 cttctttttt atccaatgca cctacatcaa tggtgatatt ctttacactt ggtgtagtat
     2941 ctttcgacat atcgcgtaag
//
LOCUS       DAOQII010000293         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_23009_length_2933_cov_4.567408, whole genome shotgun sequence.
ACCESSION   DAOQII010000293 DAOQII010000000
VERSION     DAOQII010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23009_length_2933_cov_4.567408"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1444)
                     /gene="murD"
                     /locus_tag="PLF48_11115"
     CDS             complement(101..1444)
                     /gene="murD"
                     /locus_tag="PLF48_11115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNF49924.1"
                     /translation="MNTKNNIVILGSGESGVGAAILAKKQGMGVFVSDLGKIKEEYKN
                     ELLALNIPFEEEQHSEDSILQASLIIKSPGIPDKAPLIKKAKEKNIPIISEIEFAYRY
                     SKSKIIAITGSNGKTTTTSLIYHILEKAGFDVGLGGNIGTSFARQVAHGDKAFFVLEI
                     SSFQLDGCVDFKPHIAVLCNITEDHLDRYDYKFENYINSKLSITKNQTQEDYFIYCAD
                     DEVTMKYLHTVNANVNKIPFSYYIKVSEGAYAEDEKIIIELNKNNFTMPINELALQGI
                     HNTYNSMAAAIAAKLEGVRNEKIKESLEDFKSLEHRLEFVASVRSVDFINDSKATNVN
                     STWFALESMTKPTVLILGGVDKGNDYTLIKDLVKQKVKAIICLGKDNEKLHQAFADEV
                     GYIVDTQSMQQAVAMAYNIAEKGDTVLLSPCCASFDLFENYQDRGRQFRRCVQNL"
     gene            complement(1520..2821)
                     /gene="mraY"
                     /locus_tag="PLF48_11120"
     CDS             complement(1520..2821)
                     /gene="mraY"
                     /locus_tag="PLF48_11120"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNF49925.1"
                     /translation="MLYYLFKYLEQHFDMAGAGLFQYISFRASMSILLSLIISMMIGR
                     KIIRWLRRMQIGESVRDLGLEGQIQKKGTPTMGGIIIIAAIVIPTLLFARLDNVYVIL
                     LLVSTLWMGAIGFIDDYIKVFKKDKEGLRGKFKIMAQVVLGLIVGCTLYFNKNVSVRD
                     FNSVFVYNIDKDGKVDFSRPESEEACATVMYENKGIQVGDKFLSHETRAVKTNLPFAK
                     NNLLDYAYFLKPFTDDYQKWGWLIYIPFVIFIITAVSNAANLTDGIDGLASGVSAIVL
                     ITLAIFAYVSGNIKIADYLNVLYIPNSAEVVIVCAALVGATIGFLWYNAFPAQVFMGD
                     TGSLMLGGVIATVALIVRKELLIPILCGVFFAENLSVMLQVAYFKYTKKKYGEGRRIF
                     KMAPLHHHFQKSGYHEAKLVVRFWIVSILLAIITFVTLKIR"
BASE COUNT          854 a          579 c          499 g         1001 t
ORIGIN      
        1 ggcaattata taccacacat atttgtctcc tttgatgtta ttgatgatgc gagccagcat
       61 tttttagtga ttggtgattg gtgattagga agaatgatta ttatagattt tggacgcaac
      121 gtctgaactg gcgccctctg tcttgataat tttcaaacaa atcgaagctg gcgcagcatg
      181 gcgataacaa tacggtatcg cctttttcag caatattata tgccatagcc acagcttgtt
      241 gcatactttg tgtgtcaacg atatagccaa cttcatcggc aaatgcctga tgcagtttct
      301 cattgtcttt acccaaacaa ataatagctt ttactttttg tttgactaaa tcttttatca
      361 atgtatagtc gttaccttta tcaactcccc caagtattaa tacagtgggt tttgtcatag
      421 attcgagtgc aaaccaggtt gaattgacat tcgtcgcttt tgaatcatta ataaaatcta
      481 cacttctgac agaagcaaca aactccagcc ggtgctcaag gcttttaaaa tcttccagac
      541 tttctttaat tttttcgttt cttactcctt ccagttttgc agcaatggca gccgccatag
      601 aattgtaggt gttgtggatg ccttgtaagg caagttcatt gattggcata gtgaagttgt
      661 ttttattgag ttctataata attttttcgt cttcggcgta agcaccttcg cttactttta
      721 tgtaatagga aaatggtatc ttatttacat ttgcatttac ggtgtgcaga tatttcattg
      781 ttacttcgtc atctgcacaa taaatgaagt agtcttcttg tgtttgattt tttgtgatgg
      841 ataatttaga attgatgtaa ttttcaaact tgtaatcgta tctgtctaga tggtcttccg
      901 tgatattgca caatactgcg atatgcggtt taaagtcgac acagccatct aactgaaatg
      961 agctgatttc taaaacaaaa aatgctttgt cgccatgggc tacctgacgg gcaaatgaag
     1021 taccgatatt cccgcctaat cctacatcaa aaccagcttt ctctaatatg tgataaatca
     1081 atgaggtggt agtagtcttg ccgttagagc ctgtaattgc aataattttg ctcttgctgt
     1141 agcggtaggc aaattcgatt tcagaaatta tcggaatatt tttttctttt gcttttttaa
     1201 tcagtggtgc tttatcaggt attcccggac ttttgataat gagtgaagcc tgaagtatgc
     1261 tgtcttccga atgttgttct tcttcaaaag gaatatttaa cgccagcaat tcatttttat
     1321 actcttcttt tattttaccc aagtcggaga caaatacgcc catgccctgc ttttttgcaa
     1381 gtatggctgc gcccactccg ctttcgccac tccctaggat tactatgtta tttttagtgt
     1441 tcaatattta atgtttagtt gttggtcatt agtcattggt acattggtat attggaaaat
     1501 ttgtacattc ctttattttt tatcttattt taagtgttac aaatgtgatg atcgcaagca
     1561 ggatgcttac tatccagaag cgcactacta gttttgcttc gtgataacct gacttttgaa
     1621 aatgatgatg caatggtgcc atcttaaata ttctgcgtcc ctcgccgtac tttttcttgg
     1681 tgtatttgaa gtaggccact tgcagcatga ctgataaatt ctctgcaaaa aacaccccac
     1741 ataaaattgg tatcagcaat tcttttctta caatcaatgc tactgtagca atgactccac
     1801 ccaacatcaa gcttccggta tcgcccataa atacttgtgc cggaaaagcg ttataccata
     1861 agaaacctat agtggcaccc accagtgcgg cgcatacaat tactacttcg gcagagttgg
     1921 ggatgtataa gacattgagg tagtcggcta tttttatatt accggaaaca tatgcaaata
     1981 ttgccagcgt tattaaaact atggcagaca cacccgaagc taaaccatct attccgtcag
     2041 tcagattagc tgcattagaa actgccgtga tgataaaaat gacaaacgga atatatatca
     2101 accatcccca tttttgataa tcatctgtaa atggttttaa aaaataagca taatccaata
     2161 agttattttt ggcaaaaggc aaatttgttt ttaccgctct ggtttcgtgt gacaaaaatt
     2221 tatcacccac ttgtatgcct ttattttcat acatcactgt tgcacatgct tcttctgact
     2281 cagggcggct aaaatcaact ttaccatctt tatcaatatt ataaacaaag acagaattaa
     2341 aatcacggac acttacattc ttattaaaat acaaagtgca accgactatc agacccaata
     2401 ctacttgcgc cataatttta aatttgccac gcaatccttc tttgtctttt ttgaatactt
     2461 tgatgtaatc atcaataaaa ccaatagccc ccatccaaag tgtagagact aacaacaaga
     2521 ttacatatac attgtctaag cgggcaaaca acagcgtagg aatcactata gctgcgataa
     2581 taattatacc acccatagta ggtgtacctt ttttttgtat ttgaccttcc aagcccaaat
     2641 cacgtacaga ttcaccaatt tgcattcttc gcagccaacg aataattttt ctgcctatca
     2701 tcatggagat aatcaaagac aacagaatag acatagatgc acgaaatgag atatactgaa
     2761 acaaaccggc accagccatg tcaaaatgtt gttcgagata tttgaataaa taatacagca
     2821 tccgttttat tttttaaatt ctgtaaatgt ttgagtcaat atttttttgt catcgaaatc
     2881 gtattttact ccatttattt cctgatattt ttcgtgacct tttcctgcaa gca
//
LOCUS       DAOQII010000294         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_23100_length_2926_cov_4.258447, whole genome shotgun sequence.
ACCESSION   DAOQII010000294 DAOQII010000000
VERSION     DAOQII010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23100_length_2926_cov_4.258447"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PLF48_11125"
     CDS             complement(<1..1409)
                     /locus_tag="PLF48_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF49926.1"
                     /translation="MAAFAQNSLTQNKTIDSINQESFSFAETNAEKAMQLAFKAIKMS
                     KDEHYPNGIAKAYNNIGIVFDVKGNYDSSLYYYNNGYEIADASNSLKTKAGIINNIGM
                     IYWNKGNFDFALKKYFEAEKIYAQLKEEKGRANTLSNIGLIYYDIKQYKQSFDYCFAA
                     LQIREKIKDEYGMSVSYSNIASLYSEKKDYTQSISYLEKSIEIKKKIEDDYGLAIDYT
                     NLASDKFKQTKNHQTALDLNLEAVKIRKKIDDKFGLCTSFINIAHIYIDEKKFTNALE
                     YLKAAEKLAIEMDAKSKLKEVYDAKSVLFKLTSQYDSALFYADKREVLKDSIFTEESN
                     KQYAKLHVQYETEKKENEIVQQKLVIGKRNTLVALLTAILFFSIILYYVLYRNYQIKQ
                     EKKLQQQRLEEQEKRAQAIIETEENERQRLARELHDGVGQLLSATRMNLASVTQENAT
                     QQINKSIAVLDDSIQEIRH"
     gene            1533..2498
                     /gene="pfkA"
                     /locus_tag="PLF48_11130"
     CDS             1533..2498
                     /gene="pfkA"
                     /locus_tag="PLF48_11130"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNF49927.1"
                     /translation="MKKIAVFTSGGDAPGMNAAIRAVVRCGLQNNLEVVGIQNGYEGM
                     IDGDFVTLSSRSVGNIIHRGGTILKTSRSERFLTEAGRKTAFDQLRLHQIDGVIAIGG
                     DGTFKGATVFTQEYDIPFIGLPGTIDNDLAGTEYTIGYDTAINTAMQSMDKIRDTATS
                     HGRLFFIEVMGRDSGAIALDSGIASGAEDIFIPEIKTDLEEFSAKLQLSKHKESYMIV
                     VSEGDELGHVKDIAEKFQKMHPEYEIKFVVLGHLLRGGSPTAIDRIIPSKMGIAAVEA
                     LLNNERNVMIGWKENNLHKTALTHAVKRQMEINKPLLAVLNALSY"
     gene            2517..>2926
                     /locus_tag="PLF48_11135"
     CDS             2517..>2926
                     /locus_tag="PLF48_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49928.1"
                     /translation="MSIHIGAKKGDIAEIVLMPGDPLRAKHIAENYLTDITCYNEVRN
                     MLGFTGYYNGKRVSVQGSGMGIPSIGIYATELIREYDVKRLIRVGTAGALQPHIHVRD
                     IVVAMSASTTSNINNRFFNGTDYSPTANFDLFIQ"
BASE COUNT          879 a          499 c          533 g         1015 t
ORIGIN      
        1 atatgccgga tctcttgaat ggaatcatcc aaaacagcaa tagatttatt gatttgctga
       61 gtagcattct cttgtgttac agatgctaaa ttcattcgtg tagcactaag caactgccct
      121 accccatcgt gtagttctct ggctaaacgc tgccgctcgt tttcttctgt ttctatgatg
      181 gcttgcgccc gtttttcctg ttcttctaaa cgctgttgtt gtaatttttt ttcttgttta
      241 atttgataat ttctatacaa cacataatac aagataatac tgaaaaataa aatggcagtg
      301 agcaatgcaa ctaaggtatt gcgcttaccg ataactagct tttgttggac aatttcattt
      361 tccttttttt ctgtttcata ctgcacatgt aatttggcat attgtttatt gctctcttcc
      421 gtaaaaatgc tgtctttgag aacttcacgt ttatcagcat aaaataaagc tgaatcgtat
      481 tggcttgtta gtttgaataa tactgatttt gcatcataaa cttcttttag cttagatttt
      541 gcatccattt caatggcaag tttttctgct gctttcagat attcaagagc atttgtaaat
      601 ttcttttcgt caatatagat gtgagcaata tttataaagc tggtacacaa gccgaattta
      661 tcatcaattt tctttctgat ttttacggct tctaaattca aatctaatgc cgtttgatga
      721 ttttttgttt gtttaaactt atctgaagct aaattggtat agtctatggc caatccataa
      781 tcatcttcta tttttttctt tatttcaatt gatttttcca aataactgat ggattgagta
      841 taatcttttt tttcgctgta taggcttgca atattgctgt acgaaacact catgccatat
      901 tcatccttta ttttttcacg aatttgcaag gctgcaaaac aataatcaaa agattgttta
      961 tattgcttga tgtcataata aatcaaaccg atattgctga gtgtattggc tcttcctttt
     1021 tcttctttca attgggcata tattttctca gcttcaaaat attttttcaa ggcaaaatca
     1081 aaattccctt tattccaata aatcatcccg atattattaa taataccggc ttttgttttc
     1141 aagctgttcg aagcatctgc tatttcataa ccattgttat agtaatataa ggaagaatcg
     1201 taatttcctt ttacatcaaa cacaattcct atattattat atgcctttgc aataccattc
     1261 ggataatgtt cgtctttact catctttatg gctttaaagg ccagctgcat ggctttttca
     1321 gcatttgttt ctgcaaacga aaagctttcc tgattaatag aatcaatggt tttattttgt
     1381 gtcaatgaat tttgagcaaa agctgccatg cagactatgc tgaaattcag aagggtgtat
     1441 gttcttttca atagatttac tttgcattct aaaattacac ttttcaaaat atagttggga
     1501 tttaaaaatt cagaattgct tatatttatc gggtgaaaaa aattgctgtt tttacatccg
     1561 gaggagacgc accggggatg aatgctgcca ttcgtgcggt agtaagatgc ggtttacaaa
     1621 acaatctgga agttgtcggt attcagaatg gctacgaagg tatgatagat ggtgattttg
     1681 ttacactttc ttctaggtcg gtcggaaata taattcatcg tggcggcacg attcttaaaa
     1741 catcccgtag cgaaagattt cttacagaag ccggaagaaa aacagcattt gatcagctcc
     1801 gcttgcatca gatagatggc gtaatagcca ttggaggaga tggtactttt aaaggtgcaa
     1861 cggtttttac ccaagaatat gatattccat ttatcggctt gcccggaaca attgataatg
     1921 atttagccgg cactgaatac accataggct acgatactgc tatcaatact gctatgcagt
     1981 ctatggataa aataagagat actgcaacat cccatggccg tttgtttttt attgaagtaa
     2041 tgggaagaga ttctggggca attgccttag acagtggcat tgcctcgggt gcagaagata
     2101 tttttatccc tgaaataaaa acagatttgg aagagttttc tgcaaaattg caattgagta
     2161 aacacaagga atcttatatg attgtagttt cggaaggaga tgaactcgga catgtaaagg
     2221 atattgctga aaaatttcag aaaatgcatc cggaatatga aatcaaattt gttgttttag
     2281 ggcatttact acgtggaggt agtcccacag caattgaccg gattattccg tctaaaatgg
     2341 gaattgcggc tgtcgaagct ttattgaata atgaacgtaa tgtgatgata ggctggaaag
     2401 aaaacaactt gcataaaact gcattgacac atgccgtaaa aagacagatg gaaataaata
     2461 aacctttatt ggctgtttta aatgctttat cttattaata attaaaaata aaatttatga
     2521 gcatacatat cggagcaaag aaaggagata tagcagaaat cgtattgatg cccggcgatc
     2581 ctttgcgtgc caagcatatc gcagaaaatt acctgacaga tattacttgt tataatgaag
     2641 taagaaatat gctgggcttt acaggttatt ataacggtaa aagagtttca gtacaaggct
     2701 caggaatggg tattccttct attggtattt atgctacaga acttatcaga gaatatgatg
     2761 taaaacgcct aattcgtgtt ggcacagccg gtgcattaca accgcatatt catgtcagag
     2821 atatcgttgt ggccatgtcc gcatctacta catccaatat caataatcgg tttttcaacg
     2881 gcacagacta ctctcctacg gcaaattttg atttgtttat acaatg
//
LOCUS       DAOQII010000295         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_23906_length_2866_cov_4.572750, whole genome shotgun sequence.
ACCESSION   DAOQII010000295 DAOQII010000000
VERSION     DAOQII010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23906_length_2866_cov_4.572750"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..718)
                     /locus_tag="PLF48_11140"
     CDS             complement(173..718)
                     /locus_tag="PLF48_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF49929.1"
                     /translation="MQYAPLSADDLEMLIPKFKSKLIKKNEFLLKEGEVCKDLVFVEK
                     GCLRLYYIHDDMEVSVWFALNNNSAIEIHSFISQTPSNYFLQAIEDTSLLFLPKTEID
                     KLYTTYPIMQELMRRYWEDVILNLLQRFTALEKDSAEKRYLDLLNKPELLQTIPQKYL
                     ASFIGVTPTSLSRIKKNLVKK"
     gene            complement(813..1907)
                     /locus_tag="PLF48_11145"
     CDS             complement(813..1907)
                     /locus_tag="PLF48_11145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF49930.1"
                     /translation="MLSFWENKLNLKADFIVVGAGIMGCSVAFELREKYKHAKIVILE
                     RGILPTGASTKNAGFACFGSVTEILSDIKKMGLQDALQLVKDRRAGLSILSSRLSDQQ
                     MGMTRCGGYELILQDVVSADEIFGLNKELLDSFHEEVFEDISTEIKSFGFSSDVRQLI
                     RCKQEAQIDSGNTLMHYWNLLHEKNITIITGAEVEYIDGNKLFLKDGIKFSADKIIFC
                     TNAFTKCLQLKNEIKPGRGQVIVTNEIENLKIKGSFHFDEGFYYFRNIGKRILFGGGR
                     NLDFQKEETTAFETNEIIVNDLLQKLKKLIIPNTHFEIEAHWQGIMAFSENKLPSIQA
                     INSYADYVMACNGMGVALSPLIAKQVVSVL"
     gene            complement(1926..>2866)
                     /locus_tag="PLF48_11150"
     CDS             complement(1926..>2866)
                     /locus_tag="PLF48_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49931.1"
                     /translation="TYLAQKFCPKGYIFIFPKSEEGLPPNHGEFGKDEVFCALDMVRR
                     DTLTNDFFNGIVADKKAIGGHSMGGGAAFLGLQNNPDIDAFFTLAPAETFIGGSAVTA
                     AKTIRIPGLVFTGTLDKVAPRGGNGGSMYNNLISTCKTLIEIKGASHCGFSNETPDVC
                     EVGELAVCLGCSFISNADQHATVFKYLEPWLNYWLKGTSSGLSTIATQGVTDTKIIFT
                     NSCTVTAIKNYDKLDDKSIQLFPNPTSTSLQVQSIYAVSKIEIWSMDGKLMQEVENKW
                     MPNEVIQLPVLNFQKGMYLVRSYTDQGVAVKSIIIQ"
BASE COUNT          885 a          566 c          439 g          976 t
ORIGIN      
        1 tccggtatgt aaatgataaa agaagagata ttacaacatt tccggctgca tttcatttct
       61 tattatctct ggtactcttt ccggctttaa ttcaattatt aagtcagcta aaacctgttt
      121 tttacgataa gctggcaaaa gtgcaaatta taaagatgaa gtaaaaaagc tcctattttt
      181 ttactaaatt tttttttatt ctactgagcg aagtaggtgt tacacctatg aacgaagcca
      241 ggtatttttg tggtattgtt tgtaatagtt ctggtttgtt taacaaatcc agatatcgtt
      301 tttcggcact gtctttttcc agagcagtaa agcgttgcag taaattaaga atcacatctt
      361 cccaatatct gcgcattaac tcctgcataa tcggatatgt agtatagagt ttgtctattt
      421 cagtttttgg caaaaacaaa agggatgtat cttctatagc ttgtaaaaaa tagttgctgg
      481 gtgtctgact gataaaacta tgtatctcaa tggctgaatt attatttaag gcaaaccaga
      541 cagaaacctc catatcatca tgaatatagt acaaacgtaa acaacctttt tcgacaaaaa
      601 ccaaatcctt gcatacttct ccttctttta acagaaattc atttttcttt atcaacttag
      661 atttgaattt tggtatcagc atctccaaat catccgcact cagcggagca tattgcatca
      721 aaaaatgttt aagctcagaa tgcattagat ctacaaatct gctttaaata tagcttataa
      781 tttggaataa tgagtaatgt gctcacatca aactaaagaa cagacactac ttgttttgca
      841 atcaaaggtg ataaggctac ccccatgcca ttgcaagcca tgacgtaatc tgcataacta
      901 ttgatggctt gtatgcttgg gagtttattt tcgctgaatg ccatgatgcc ttgccagtgt
      961 gcttctattt caaaatgagt atttggaata atcaactttt ttaatttctg aagtaaatca
     1021 tttacaataa tttcattcgt ttcgaatgca gtagtttctt ctttttgaaa atctaaattt
     1081 cttccaccac caaacaaaat gcgtttgccg atattcctga aatagtaaaa gccttcatca
     1141 aaatgaaaac tgcctttaat ttttaaattt tcaatttcgt tggttacaat cacttgtcct
     1201 ctgcccggtt ttatctcatt ttttagctgt aagcatttcg taaaagcatt ggtacaaaat
     1261 ataattttgt ctgcactgaa ttttattcca tcttttaaaa aaagtttatt gccatctata
     1321 tattcaactt ctgcacctgt gataatcgta atattttttt cgtgcaataa attccaataa
     1381 tgcatcaagg tgttgcccga atcaatttgt gcttcttgtt tacagcgtat aagttgccta
     1441 acatcactcg aaaatccaaa tgattttatt tctgttgaaa tatcttcaaa tacttcttca
     1501 tgaaatgaat cgagtaattc cttattcagc ccaaaaatct catctgcact caccacatct
     1561 tgcaagatta attcatagcc gccacatctt gtcattccca tttgttggtc actaagtctg
     1621 gacgataaaa tggacaagcc tgctcttcgg tctttcacta actgcagggc atcttgcaga
     1681 cccatttttt taatatcact taatatttct gttacagagc caaagcaggc aaaacctgca
     1741 ttttttgtgg atgcacctgt gggtaaaata cctcgttcca gtataacgat tttagcatgt
     1801 ttatattttt cacgaagttc aaaagctact gaacagccca tgatacctgc tccgacaacg
     1861 ataaaatctg cctttagatt tagcttattt tcccaaaaag acaacatcta acaggaagaa
     1921 gttatttatt gaatgataat tgacttaact gcaactcctt ggtctgtata acttcttacc
     1981 aaatacatgc ctttttgaaa gtttaatacc ggtagttgta tcacttcgtt tggcatccat
     2041 ttgttttcaa cttcctgcat aagttttccg tccatgctcc aaatttcaat ttttgaaacc
     2101 gcataaatag attgcacttg taaggaagtt gaggtgggat ttggaaaaag ctgtatgctt
     2161 ttatcatcca gcttatcata gtttttaatg gctgtaacgg tacaactatt agtgaaaata
     2221 attttagtat ccgtaacccc ttgtgtagct atagtactta atccgctact agtacctttt
     2281 aaccaataat taagccaagg ttctaaatat ttaaatactg ttgcatgttg atctgcattt
     2341 gaaataaaac tacatccaag acatacagcc aattctccca cttcacacac atccggtgtt
     2401 tcattagaaa agccacagtg cgatgcccct ttaatttcta ttaaagtctt acatgtagaa
     2461 atcagattat tatacatgga accaccattt ccacctcttg gagctacttt atccagcgtt
     2521 ccggtaaaga ctagaccggg aatacgtatt gtttttgcag ctgttaccgc agatccacca
     2581 ataaaagtct cagcaggtgc taatgtgaaa aatgcatcga tatccggatt gttttgcaag
     2641 cctaaaaaag cagcgccgcc acccattgaa tgtccgccaa tagctttttt atcagcaaca
     2701 atgccattaa aaaaatcgtt tgtcagtgta tctcttctta ccatatctaa ggcacaaaag
     2761 acttcgtcct taccaaactc accatgattt ggcggcagac cttcctcaga ttttggaaaa
     2821 ataaaaatat aacccttcgg acaaaatttt tgtgctaaat aggtaa
//
LOCUS       DAOQII010000296         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_24034_length_2856_cov_5.356658, whole genome shotgun sequence.
ACCESSION   DAOQII010000296 DAOQII010000000
VERSION     DAOQII010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24034_length_2856_cov_5.356658"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(775..2214)
                     /locus_tag="PLF48_11155"
     CDS             complement(775..2214)
                     /locus_tag="PLF48_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49932.1"
                     /translation="MKKTNLLLPAIAFIVILIAGCSKSDDNSNPVTPTDKPVKTVTGT
                     LSGNITWDKDTIYKLSGYVRVGSDDGTTMDNASGVKGTLTIEAGTIIIGEKASKGTLI
                     IQRGSKLIAEGTAANPIIFTSEKAQGARAPGDWGGLVICGLAPINIAGGHSELEGGYG
                     AYFGGNIANDNSGILKYVRVEYAGIAINPNQEINTFTFGGVGSGTTLSYLQASYAGDD
                     SYEWFGGTVNADHLIAYRGTDDDFDTDNGYSGKVQFGLGVRDKNIADYATGGASNGFE
                     SDNDATSSSAAPFTSAEFCNMTIIGPKKDTSTTIGTPFKNAALIRRNSRLKIYNSVLI
                     GYPNGISIDGATTIANAQAGDVAIKNTVIAGTLGTGNLGYFVLGDVNIPSGHTWASAT
                     WSTVRNWYLDVAFHNSIAANNRWDNLGIQASVFDIDAPNFLPNAGATILSGAGTFSGF
                     TGTTYIGAFGTDNWTNTWANFNPSVTKYL"
     gene            complement(2234..>2856)
                     /locus_tag="PLF48_11160"
     CDS             complement(2234..>2856)
                     /locus_tag="PLF48_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF49933.1"
                     /translation="NGEGISLAGFYKHFINPIETFTKDLGNRTFSFQNAQNAQAYGLE
                     IEVRKNFSRTTTFLKDFSLVLNASYIKSKVHLDSTQALGQSNNRPLQGQAPFMVNAGI
                     FYNNKDKRIQFNLLYNITGKRILYVGAQDYPDIYEMPRHSLDFNASYTFKKQVELSLG
                     LTDLINQQYVLLQDGNSDRKLKRKQDQVFQSYKTGTQISLGVKYTF"
BASE COUNT          851 a          553 c          539 g          913 t
ORIGIN      
        1 tttaacaacg ataaacctat tcttaacaaa attgcgttta ttacttgaat tcagcactta
       61 cataaattca gtttattctt aaaaagtatt tatatttgta gaatgaaacg cttattctta
      121 atcttagttg ctattgtttt tattagctct tgcaaaaaat atggatccga attaaactat
      181 ggcaaagatg gctgcataga ttgtaaatta gtaatggata cgatttcgag cagtgatact
      241 atatcgtatg caatgcccta tgctgaatgg tatctaaaga atcaccgtac ggattggtgt
      301 aaatatttag atagtcaaat cggacagact atacacttat ctgactcagt tagagttgaa
      361 ctagctgtta agtgtaatta aatctataaa caaaaccgct acttaggaaa ataaaaaaat
      421 cccactcatc gtgggaagtc ttcatgaaaa gaaattgtcc ggttgcaata caaaagtata
      481 atggctttat gttgtttaag tgaaaatcaa atgcatttta gatgaaaaaa aatcgccgag
      541 ctactcaccc ggcgttcaca cagatatatg gaattcaaga ttccataatg atattgttct
      601 gaaacaatac tgcaaagtaa caacataaaa cagtgtgaaa tgtgaacgta atgtgtattt
      661 attatgtaat tttgtatccg attgcggcac aaataaaaac accggagtaa ggactccggc
      721 gttcacacag atgaatgcac gctgtcgctc acacacttgc gacagcggat gctatcagag
      781 atattttgta actgacgggt tgaaattagc ccatgtattt gtccagttat cagtaccaaa
      841 tgcaccgata taagttgttc cggtaaatcc ggagaatgta cctgcgccac ttaagatagt
      901 agcaccggca ttaggtaaga aattaggcgc atcaatatca aaaacacttg cttgaatgcc
      961 taaattatcc catcggttgt ttgcagcaat gctgttatga aatgcaacat ctaaatacca
     1021 gtttctcact gttgaccatg tggcagatgc ccatgtatga cctgatggga tattgacatc
     1081 acccagaaca aaataaccta aattaccagt gcctaaggtg cctgcaatga ctgtgttttt
     1141 tatggcaaca tctccggctt gagcattagc aattgtagtt gcaccatcta tagaaatgcc
     1201 atttggatag ccaatcaaaa cggaattgta tattttcagt cttgaatttc ttctgattaa
     1261 tgcagcattt ttaaaaggcg ttcctatcgt tgtagaggta tcttttttag gaccgataat
     1321 cgtcatgtta caaaactcag cagatgtgaa gggtgcagcg gaagaggatg ttgcatcatt
     1381 atccgattca aacccgtttg atgcaccgcc tgttgcataa tcggcaatat ttttatcgcg
     1441 tacacctaag ccaaactgaa cttttccgct atatccattg tccgtatcaa agtcatcatc
     1501 tgtacctctg taagcaatta aatgatcggc atttaccgtg cctccaaacc attcataaga
     1561 atcatcacct gcataagatg cctgtaagta gcttaaagta gtaccgcttc ccacaccacc
     1621 aaaggtaaac gtattgattt cctgattggg attgattgca atgccggcat actcaacacg
     1681 cacatacttt aatatacctg agttgtcatt tgctatatta ccaccgaaat aggcaccata
     1741 gccgccctct aattctgagt gtccgcctgc aatattaatt ggagctaaac cacagattac
     1801 tagtccaccc cagtcgccgg gtgcacgtgc accttgtgcc ttttctgaag taaaaataat
     1861 cggatttgca gcggtacctt cagctattag cttgcttcct ctttgtataa taagcgtacc
     1921 tttgcttgct ttttcaccaa taataattgt accggcttca atcgttaatg tccctttgac
     1981 tccggaggcg ttgtccattg ttgtgccatc atcagaacct acccgaacat atccggatag
     2041 tttgtaaata gtgtctttgt cccatgtaat atttccgctt aatgtgcctg tcaccgtttt
     2101 tacaggttta tccgttggag ttacaggatt gctgttgtca tcgctcttgc tgcaacctgc
     2161 tattaaaatg acgataaagg cgattgccgg taataataag tttgtttttt tcataaattg
     2221 attttgagtt tatttaaaaa gtatatttta caccaagaga aatttgtgtt cccgttttgt
     2281 aggactgaaa tacttgatct tgtttgcgtt ttaattttct atcgctgttg ccatcttgca
     2341 acaaaacata ttgctgattg attaagtccg ttaagcctaa gcttaactca acttgttttt
     2401 taaaagtata agatgcattg aaatctaaag aatgtctggg catttcatag atatccggat
     2461 agtcttgtgc gcctacataa aggattcgct taccggtaat gttatagagc aaattaaact
     2521 ggattcgttt gtctttatta ttataaaaga taccggcatt taccataaag ggagcttgtc
     2581 cctgcaaagg tctgttattc gattgaccga gtgcctgtgt gctgtccaga tgaactttgc
     2641 ttttaatata gctggcattc agtaccaggc taaagtcttt taaaaaggtt gtggtgcgac
     2701 tgaagttttt gcgcacttca atttctaagc cataggcttg tgcattttgc gcattctgaa
     2761 atgaaaaggt acggttgccc aaatcttttg taaaggtttc gataggattg ataaagtgtt
     2821 tataaaatcc ggcaaggctg attccttctc cgttag
//
LOCUS       DAOQII010000297         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_24368_length_2832_cov_4.900612, whole genome shotgun sequence.
ACCESSION   DAOQII010000297 DAOQII010000000
VERSION     DAOQII010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24368_length_2832_cov_4.900612"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /gene="ftsZ"
                     /locus_tag="PLF48_11165"
     CDS             complement(<1..763)
                     /gene="ftsZ"
                     /locus_tag="PLF48_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNF49934.1"
                     /translation="MEFEMFSEHNSIIKVLGVGGGGCNAVNYMYEQGIPGVNFGVCNT
                     DKQDLLKSPVPTKMQLGTNLLEGLGAGADPEDGRRAALEAEEEIRMYLSNNTKMVFVT
                     AGMGGGTGTGGAPVVAKIAKDLGILTVGIVTLPFSNEGKPKRDKAEKGLHNMKENVDA
                     LIVVSNNKLYDIYPDLDIDEAFSKADDILTTAAKGVTEIITRPGKINVDFNDVRRVMS
                     NSGVAIIGIGRGAGEDRAMNAINDAMNSPLLEDADI"
     gene            complement(813..2264)
                     /gene="ftsA"
                     /locus_tag="PLF48_11170"
     CDS             complement(813..2264)
                     /gene="ftsA"
                     /locus_tag="PLF48_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNF49935.1"
                     /translation="MHVEKDIDMTEQQNQQNIVCAIDIGTSKVAALVGRRNQFGKIEI
                     LGIGNCPSFGVQRGVVVNIARTVDSIKKAVAEAERQSGVTFEKVHVGIAGQHIKSLQH
                     RGILTRSNNDTWINYEDIQRLKNEMYKLALPPGDRILHVLPQEYIVDNEPGIYHEPIG
                     MMGIRLEGNFHIITGAENAIQNIERCIHIAGLEVASIELEPLASADAVLSKEELEAGV
                     CLVDIGGGTTDIAIFENYVIRHTAVIPFGGNIITEDLRDFRILRDQAEDLKKRFGSAI
                     PYDELRNQHVSIKGINNTGNRELNLLSVSKVISARVKEILAHVDFQIKLSGFGKKLIG
                     GIILTGGGSQLKHLIQLTESETGQSARVGLPTEHLASTKLVNIENPIYATAIGLLIRG
                     LKIQDEKALSEQQYIPKKEEIKIVAEEQPVVVAPEQTTPVLNEIKLEKEVVEEEITIS
                     DDKSKLNEGSLISVWGKKLGSWLRSDVDDFDNK"
     gene            complement(2276..>2832)
                     /locus_tag="PLF48_11175"
     CDS             complement(2276..>2832)
                     /locus_tag="PLF48_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49936.1"
                     /translation="NKLNGNDLEEIEDTIAALPLVKKANAYIDNNGNLNIQIEQRRPL
                     LRVFNSINETYYIDEDGVKFPCSKLYTARVPIVNGNIPEQCGKEQKIQSKELKRVFAV
                     WNGIKENDTWKNLIGQLFVNQKSEVELVPRIGSAIIYFGNDQDIPQKIKRMDVFYFDV
                     LPKVGWNTYKVINIMYKDQVICKK"
BASE COUNT          737 a          647 c          476 g          972 t
ORIGIN      
        1 aaatatccgc atcttcgagc aacggtgaat tcatggcatc gttgatagca ttcatcgccc
       61 tgtcctctcc tgcccctctg cctataccaa ttatagccac accgctattg ctcatcacac
      121 ggcgcacatc attgaagtcc acattgattt tacccgggcg tgtaataatt tccgtaacgc
      181 cttttgcagc tgtcgttaaa atatcatctg ccttagagaa ggcttcatca atatccaaat
      241 caggataaat atcatacagt ttattattgg acactacaat taatgcatcc acattttctt
      301 tcatgttgtg caaacctttc tcggctttat ctcgtttagg ttttccttca ttactaaaag
      361 ggagcgtaac aatacccacc gtaagtatgc ccaaatcttt ggcaattttt gctaccacag
      421 gcgcaccacc cgtacctgtc ccaccaccca tgccggcagt aacaaacacc attttggtat
      481 tgtttgataa gtacatgcgt atttcttctt cggcttccaa ggctgcacgt ctgccgtctt
      541 cggggtcagc gcctgcacct aggccttcca ataaattggt acctaattgc attttcgtag
      601 gtaccggact ttttaataaa tcctgtttat cggtattaca aacaccgaaa tttactccgg
      661 gtatgccttg ttcatacata taattcacgg cgttgcaacc accgccgcca actcccaata
      721 ctttgatgat agagttgtgt tcgctaaaca tttcaaattc catggttgta tattttattt
      781 ttttactgtt attgtttttt tacaaaaatg atttatttat tatcaaaatc atccacatca
      841 gaacgcaacc aacttcctaa ttttttaccc cacacagaaa tcaagcttcc ctcatttaat
      901 tttgatttgt catcactaat agtgatttct tcttcaacta cttctttttc cagtttaatt
      961 tcattcaata ccggagtagt ttgctcagga gcaacaacaa caggctgttc ttccgccaca
     1021 atttttattt cttctttttt aggaatatac tgttgttctg ataaggcttt ttcatcttgg
     1081 attttaaggc ctctgataag caaaccgatt gctgtagcat aaatcggatt ttctatattc
     1141 acgagtttag tagatgccag atgttcggta ggcaaaccga cacgtgcgct ttgtcctgtt
     1201 tcagattcgg ttaattgaat taagtgtttc agctgagaac cgcctccggt aaggatgata
     1261 ccgccaatta atttcttgcc gaatcctgat agttttatct ggaaatcaac atgcgctaaa
     1321 atttctttaa cacgtgctga aattactttt gacacgctca ataaattcaa ctcccgattt
     1381 ccggtattat tgatgccttt gatagataca tgctgattgc gcaactcatc ataaggaatc
     1441 gcagaaccaa atcgtttttt caaatcctcc gcctgatcgc gcagaatacg gaaatcgcgc
     1501 aagtcttcgg tgatgatatt tccgccaaat ggtatgactg ccgtatgacg aattacatag
     1561 ttttcaaaaa tggcaatatc cgttgtacca ccgcctatgt ccactaaaca cactccggct
     1621 tccagttctt ctttgctcaa cactgcatct gcagatgcca aaggttctaa ctcaatgctt
     1681 gctacttcca gtcctgcaat atgaatacag cgttcaatat tttgaatggc attttcagca
     1741 ccggtaatga tatggaaatt tccttccaaa cgaattccca tcatacctat tggttcgtga
     1801 taaatgccgg gttcgttatc tacgatatat tcttgcggca atacgtgtaa gatacgatcg
     1861 cccggaggca atgccagttt atacatttca tttttcagtc gttggatatc ttcgtagtta
     1921 atccaagtat cgttattgct gcgtgtcaaa ataccgcgat gctgcaagct tttgatatgc
     1981 tgccctgcaa tgcctacgtg tactttttca aatgttacac ccgactggcg ttctgcctca
     2041 gccactgctt tcttgatgga atctacagtt cgggcaatat ttactactac tccgcgctgt
     2101 acaccgaagg aaggacagtt gccgatacct agaatttcaa tcttaccgaa ttgatttctg
     2161 cgtcctacta atgcagcaac tttggatgtg ccgatatcta tggcacatac tatattttgt
     2221 tggttttgtt gctctgtcat gtctatatct ttttcaacgt gcatatttaa tatttttatt
     2281 ttttacagat cacctgatct ttatacatta tgttgataac cttgtaggtg ttccaaccta
     2341 ctttgggcaa tacgtcaaaa taaaagacat ccatacgttt tattttttga ggtatatcct
     2401 gatcattgcc gaaataaata attgctgaac ctatacgcgg tactaattcc acttcacttt
     2461 tctgattgac gaatagctga ccgattaagt ttttccatgt atcattttct tttattccat
     2521 tccatacggc aaatactctc ttcaattctt tactttgaat tttttgttct ttaccgcatt
     2581 gctccggtat attgccattc acaataggaa cccgggcggt atataatttt gaacagggaa
     2641 attttactcc gtcttcatca atatagtacg tttcgttgat ggaattgaaa acacgaagca
     2701 gtggacgacg ttgttctatc tgtatattca ggttgccgtt attatctatg taggcatttg
     2761 ctttttttac cagaggaagt gcagcaatcg tatcttctat ttcttccaaa tcattcccat
     2821 tcagcttgtt at
//
LOCUS       DAOQII010000298         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_24493_length_2824_cov_4.756591, whole genome shotgun sequence.
ACCESSION   DAOQII010000298 DAOQII010000000
VERSION     DAOQII010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24493_length_2824_cov_4.756591"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLF48_11180"
     CDS             complement(<1..235)
                     /locus_tag="PLF48_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNF49937.1"
                     /translation="MQADFGKGNGLLPAIVQDEATGKVLMLGFMNEEAFKMTNESGKV
                     TFFSRSKNRLWTKGESSGNYLDVKSVLLDCDKDT"
     gene            293..1129
                     /locus_tag="PLF48_11185"
     CDS             293..1129
                     /locus_tag="PLF48_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNF49938.1"
                     /translation="MLKLYFIKPNIDILKRSTQLELCLHLHPHCFSYLILNTENNQII
                     DFEKYSFTNPTNEILLSELKMWFTQNQEILHLPFKVSKVAIFAEIFTVLPEKSEKPAE
                     VFEMLGYNIKPDTSIFKDKIEDNFFVYYQLDDELIRFLDNQVVNAEFYCGDSGLLRFY
                     NNRIAYKSYLAAYVYGEDLVICHKKDNHNYYYNRFKIKAKEDLLYYLQLAYEQLGLDK
                     NEYPTYLYGFIEEKSPMYSTSYSYIRNFEIDRTLKNTLPFLYAVEDFPLHYFVNLWGL
                     VL"
     gene            1254..2777
                     /locus_tag="PLF48_11190"
     CDS             1254..2777
                     /locus_tag="PLF48_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF49939.1"
                     /translation="MDFLKNLGIQEINKGVSTGSAWFETSAQIDSYSPVDGKLIASVQ
                     VCDAKNYEHVVQSAQKAFAEWKKIPAPKRGEIVRQIGNEFRTHKDDLGRLVSWEMGKS
                     LQEGLGEVQEIIDICDFAVGLSRQLYGLSMHSERPNHRMYEQWHPYGIVGIITAFNFP
                     VAVWGWNAMLALVCGDVCIWKPSEKTPLSALACQFIMSKIIKENNLPEGISGMIVGER
                     EVGEWMTNDTRIPLISATGSTRMGKEVGKVVAERLGRSLLELGGNNAIIITPSADLNV
                     ALIGSVFGATGTTGQRCTTTRRLIIHSSIYDEFKTKLVAAYGQLKIGNPLEAGIHVGP
                     LIDTHAVKLYENAIQQCKEQGGRFLVEGEVLSGGNYASGCYVKPCIAEVDSQLPIVMH
                     ETFAPILYLMKYDTIEEALALQNAVPQGLSSSIMTMNMHEMEYFLSAQGSDCGIANVN
                     IGTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINFGTDLPLAQGIQFDLG"
BASE COUNT          896 a          507 c          552 g          869 t
ORIGIN      
        1 atgtatcttt atcacaatcc aataaaaccg actttacatc caaataattg ccgctggatt
       61 cacctttagt ccacaaacgg tttttagacc tgctgaaaaa tgtaacttta ccgctttcat
      121 tcgtcatttt aaaggcttct tcattcataa agccaagcat gagcactttt cctgtagctt
      181 catcttgtac aattgcgggc aataagccat ttcctttacc aaaatctgct tgcataattt
      241 ttactaatgt gtaaatatac taattccaca aattgtaacg aattccttat ttttgctaaa
      301 actttatttt atcaagccaa atattgacat cctcaagcga tcaacgcaac tcgaactctg
      361 cttgcatctt catccgcatt gtttcagcta tttgatatta aatactgaaa ataatcagat
      421 aattgacttt gaaaaatata gttttaccaa cccgactaac gaaatcctat tgagtgagct
      481 aaaaatgtgg tttacgcaaa atcaggaaat actgcatttg ccatttaaag ttagtaaagt
      541 ggccattttt gctgaaatat ttaccgtttt acctgaaaaa tctgaaaaac ctgccgaagt
      601 atttgaaatg ttgggttata acataaaacc agacaccagt atttttaaag ataaaattga
      661 agacaatttc tttgtttatt atcaactgga tgacgaactg attcggtttt tggataatca
      721 agtggtaaat gcagaatttt attgtggtga ttcaggctta ttgcgatttt acaataacag
      781 aatagcttat aaaagctatc tggccgctta tgtttatggt gaagatttgg taatttgtca
      841 taagaaagac aaccacaact attattataa tcgttttaag ataaaagcta aggaggattt
      901 gctttattac ttacaattag catatgaaca gctgggtctg gataaaaacg aatatcctac
      961 ctatttatat gggtttattg aagaaaaatc acctatgtat tctacgtcat acagctatat
     1021 ccgcaatttt gaaattgacc gtacacttaa aaacacactc ccctttttat atgctgttga
     1081 agatttcccc ttacattatt ttgtaaatct ttgggggttg gttttataat tttaaaatgt
     1141 ttttcacgct aattcctctg actctattta tattccaatc tttagcaatt tcaattacct
     1201 atcgcataaa cccctcatta atttttcata attttgcaaa ctaaaataag cgaatggatt
     1261 ttttaaaaaa cttagggatt caagaaatta ataaaggggt gtctacaggc agtgcttggt
     1321 ttgaaacaag tgcacaaatt gacagctact ctcctgttga cggcaaatta attgccagtg
     1381 tacaagtttg tgatgcaaaa aattatgagc atgtagtaca atccgctcag aaagcatttg
     1441 ccgaatggaa aaaaattcct gcgccaaaac gcggagaaat tgtgcgacaa ataggtaacg
     1501 aattcaggac acataaagat gatttaggtc gactggtttc gtgggaaatg ggaaaaagct
     1561 tacaagaagg cttgggagaa gtgcaagaaa ttatagacat ctgcgatttt gctgtagggc
     1621 tttcacgcca gctttatggc ttgagtatgc attcagaacg acctaaccac agaatgtatg
     1681 agcagtggca tccatatggc attgtgggga ttatcaccgc ttttaatttc ccggtagcgg
     1741 tatggggctg gaatgctatg ttagcattag tttgcggaga tgtatgtatt tggaaacctt
     1801 ctgaaaaaac tccattgtca gcattggctt gtcaatttat catgtcaaaa atcattaaag
     1861 aaaacaatct gccggaaggc atttccggaa tgatagttgg cgaaagagaa gttggggaat
     1921 ggatgacgaa cgacactcgt attccgctta tatcggctac gggttcaacc cgtatgggca
     1981 aagaagttgg taaagttgtt gcagaaagac tgggcagaag tttactggaa ttaggaggaa
     2041 ataatgccat tatcattacg ccaagtgccg acttaaatgt tgcactaatt ggcagtgtct
     2101 ttggagctac aggcactacc ggacagcgtt gtactaccac ccgcagactg attatccact
     2161 cttccattta cgatgaattt aaaaccaaac tcgtagcagc ttatggtcaa ttaaaaattg
     2221 gcaatccgct cgaagcaggt atacatgttg ggccgctgat tgatactcat gccgttaaac
     2281 tttacgaaaa tgctattcaa cagtgtaaag agcaaggtgg cagatttctt gtagaaggag
     2341 aagttctatc cggaggcaac tatgcatcgg gatgttatgt aaaaccttgt attgcagaag
     2401 ttgacagtca acttcccatt gtgatgcatg aaactttcgc tcccatacta tatttgatga
     2461 aatatgatac cattgaagaa gcattggctc tgcaaaatgc cgttccgcaa ggattatctt
     2521 cttccataat gaccatgaat atgcacgaaa tggaatattt tctgtctgcc caaggttcag
     2581 attgcggtat tgccaacgtg aatattggca cttcaggtgc agagattggc ggtgcatttg
     2641 gtggagaaaa agaaaccggt ggtggcagag aatcgggttc tgactcatgg aaagcctata
     2701 tgcgaagaca aaccaacacc atcaactttg gtacagactt gcctttagcg caaggaattc
     2761 agtttgattt aggataattt tggattaagc aagattatga aagacatgtt gcacgtcatc
     2821 atct
//
LOCUS       DAOQII010000299         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_24650_length_2814_cov_4.488220, whole genome shotgun sequence.
ACCESSION   DAOQII010000299 DAOQII010000000
VERSION     DAOQII010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24650_length_2814_cov_4.488220"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..346)
                     /locus_tag="PLF48_11195"
     CDS             complement(131..346)
                     /locus_tag="PLF48_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49940.1"
                     /translation="MQVEEKAFDIPHDGSLGLLAYGYIGLLAWRQARKKYELETGKKI
                     RYFHIETKNEIIAETSTKGGIENEQKA"
     gene            complement(389..973)
                     /locus_tag="PLF48_11200"
     CDS             complement(389..973)
                     /locus_tag="PLF48_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49941.1"
                     /translation="MKKNIKMYSALALGASAILPVACKKDNNDPNIIDKNIGLSLSIG
                     ASPSLKIDSIDIDGNGRTDMAFLIGTEDHDSTMISYLTSMNSNSAFIVNANYTVMTIE
                     EGNQQDAITTSSSNLGSVALFHSKNLGSSTNIGIGGQGDKYIVFAVRDAVAAPDDIHT
                     GWMKVNLSSDLKTLTIKDLAYQKNPGVGIKVGAK"
     gene            1072..2016
                     /locus_tag="PLF48_11205"
     CDS             1072..2016
                     /locus_tag="PLF48_11205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF49942.1"
                     /translation="MQTAKKYTDFENRLVKVAKYRAKQAVKLQTDAYRIYDRDLPEFP
                     FIIDKYRDALCVYEYKSKHQLTASDYKDWLNYSLEIIAKTCGVEQTYIYLKTRERKKG
                     EEQYQKLQQQKNEMIVHENGLSFIANLSDYVDTGLFFDQRIARQKIKQLATDKHVLNL
                     FAYTGSFSVYAAAGHAKTITTVDLSKTYLNWARRNLTYNKLYDEKKHFFMQEDTLSFL
                     KNIKEYFDIIICDPPTFSNSKRMYHDFDIQRDYLCLITACLKRLNPGGQLLFSTNKRQ
                     FQYDLSELKCTSWKDITKSVIPFDFEGKFGLTVLQIMK"
     gene            complement(2017..2487)
                     /locus_tag="PLF48_11210"
     CDS             complement(2017..2487)
                     /locus_tag="PLF48_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49943.1"
                     /translation="MRKLLLIFSLVLSFSAIQAQTYNVAAYTFHQVIVNEQESIGDKI
                     YDFIQNANRRNLHKLQLQIKYSSAIIDSLKPYNNETTYLDVAKKLFAYYKDVSATDYV
                     ALEKLVENSSLEPNQFQAEKQKIFNSIKQKGNAVYQPFNIAQEEFCKKYGIKLE"
BASE COUNT          936 a          480 c          451 g          947 t
ORIGIN      
        1 agggtcaata gtagcaatat tgccacaaac tccgttttct accaatgatt gtagtgcggg
       61 catttcaccg gcatccatta aaggattgat tattttccaa tcggcagaat cccagccaat
      121 caccaatact ttaggctttt tgctcatttt ctattcctcc tttagtgctt gtctctgcta
      181 taatctcatt tttagtttca atatggaaat agcggatttt cttacctgtt tctaattcat
      241 atttttttct tgcctgacgc catgccaata agcctatata gccataagct aataatccca
      301 atgagccatc atgtggaatg tcaaatgctt tttcttctac ctgcatgaag taaaaatatg
      361 aattattaat taaagtgaat cttgtaaatt attttgctcc tacttttatt cccactccgg
      421 gatttttttg ataagctaaa tctttaatag ttaaagtttt taaatctgaa cttagattaa
      481 ctttcatcca gcctgtatgg atatcatctg gagcagctac tgcatctctc actgcaaata
      541 caatatactt atctccttga ccaccaattc ctatattagt actactgccg agatttttgg
      601 aatgaaacaa agcaactgaa cctaaatttg aactactagt agtaattgca tcctgttgat
      661 taccttcttc aatagtcatg actgtataat tagcattaac tataaaagcg gaatttgagt
      721 tcatcgaagt caaataagaa atcattgtgc tatcatggtc ttctgtgcct atcaaaaaag
      781 ccatatcagt acgtccattt ccatctatgt caatcgaatc aatctttagg cttggtgatg
      841 caccaattga taatgataaa ccaatgtttt tatcaatgat attaggatca ttgttgtctt
      901 ttttacaagc aactggtaaa atggcacttg ctcccaaagc caacgccgaa tacattttaa
      961 tgttcttttt catagcattt aatttttatt taaaaataag catttttaaa aaataatcag
     1021 cttaaattta gtattcggtc attttcattt agaatttgat tattttccat catgcaaaca
     1081 gctaaaaagt atacagattt tgaaaacaga ttagtaaaag tcgctaaata tagagctaaa
     1141 caagccgtaa agttgcaaac ggatgcgtac aggatttatg acagagattt gcccgaattt
     1201 ccttttatca ttgacaaata cagagatgca ctttgtgtgt acgaatataa aagtaaacac
     1261 cagcttaccg catcagatta taaggactgg ctgaactact cgttagaaat tattgctaaa
     1321 acatgtggtg tggaacaaac ttacatttac ttaaagacta gagaacgaaa aaaaggggaa
     1381 gagcaatacc aaaaattgca acagcaaaaa aatgaaatga ttgtccatga aaatggacta
     1441 agttttatag ctaatttatc tgattatgtg gatacgggct tattttttga ccaacgcatt
     1501 gccagacaga aaatcaaaca attggcaact gacaaacatg tgctgaatct ttttgcatat
     1561 acaggcagtt ttagcgttta tgctgctgcc ggacatgcaa aaacaataac gacagttgat
     1621 ttatccaaaa cttatttgaa ctgggcaaga cgcaacctca cttataataa attatatgac
     1681 gaaaaaaagc atttctttat gcaggaagat accttaagct ttttaaaaaa tataaaagag
     1741 tactttgata tcattatttg cgacccaccc acattttcca acagcaaacg catgtatcat
     1801 gattttgata ttcaacgaga ttatttatgt ttgataaccg cttgtttgaa gaggttaaac
     1861 cccggaggtc agctcttatt tagtacaaat aaacgacaat ttcagtacga tttatcagaa
     1921 ctaaaatgca cgagttggaa agatattact aagtctgtta taccttttga tttcgaaggc
     1981 aaattcggat taacagtatt gcagataatg aaataattac tccagcttta ttccatattt
     2041 tttgcaaaat tcttcttgtg ctatattgaa tggctgatag acagcatttc ctttctgttt
     2101 gatagaatta aatatctttt gcttttcagc ttgaaattga ttgggttcaa gacttgaatt
     2161 ttcaaccagc ttctccaaag caacataatc cgttgctgaa acatctttat aataggcaaa
     2221 gagtttttta gccacatcca aataagtcgt ttcattatta taaggtttca agctgtcaat
     2281 tatggctgaa gagtatttta tctgtaactg caatttatgc agatttcgac gattggcatt
     2341 ttgaataaag tcataaattt tatcacctat actttcttgc tcattcacaa taacttgatg
     2401 aaaagtataa gcagctacat tatacgtttg tgcttgtata gccgaaaaag atagaacaag
     2461 actaaaaatt aggagcaatt tgcgcatcgt agattttttt tacaaatata gcaataaaag
     2521 tgccagatta aatttgcaaa acggtctgca tttattgaat gagtttgcag ttagcgatgt
     2581 atgtatccga atttaaattt tgtgctaatt tgtacattca acagtattag ttgtatattt
     2641 aactatgcgt ctaggtgttt tattatttat ggtcacatct tattttatgc tctcggcaca
     2701 atgcaatggg ggcagatatg aacaaaaagt attttcgcaa gtaaaagtga caaagaacat
     2761 tgtatcacat gctgcaatta atgcatcagg acggatgcaa aatataatga tgga
//
LOCUS       DAOQII010000300         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_24680_length_2812_cov_5.236852, whole genome shotgun sequence.
ACCESSION   DAOQII010000300 DAOQII010000000
VERSION     DAOQII010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24680_length_2812_cov_5.236852"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PLF48_11215"
     CDS             <1..1464
                     /locus_tag="PLF48_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF49944.1"
                     /translation="FATTAIAHIGIGTLPTLYFGTEEQKQKYLPKLASGEWAASYCLT
                     EPNAGSDALGAKTTATLSEDGKSWILNGQKMWITNAGFADLFTVFAKIDGKEFTGFLV
                     EKGTPGLSLGAEEDKLGIKGSSTRQVFFENCKIPVENMLGERGKGHLIAFNILNIGRY
                     KLGATALGGARETFNAAVRYANERYQFGKPIASFGAIQHKIADMAIKIFTLEVSSYRC
                     ANDIGTMEEELKVQGVEFAKALLESAEEYAIECSIVKVFGSEVLDFVVDENVQIHGGM
                     GYSEETAAPRAYRDSRIARIYEGTNEINRLLSIDMLLKRAMKGEIDLMTPGMAIQKEL
                     MSVPDFGSDDEGQFAAEEKALKNAKKAFLLVAGGAVQKLMAKMKDEQEIIMNCADILI
                     DIYTAESLLLRVKKIVEKGEKTDPVYIDILKVYFNDAMNRIFVNGKDALQSFAEGDEL
                     RIMQMGLKRFTKYEPVNVKEARRRIAAKVLEANKYNL"
     gene            complement(1543..>2812)
                     /locus_tag="PLF48_11220"
     CDS             complement(1543..>2812)
                     /locus_tag="PLF48_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49945.1"
                     /translation="LANFSPNDLNSLGIIKATTNNATGCLTFKFMSDGSVTKAGYRAD
                     ISCGAKRLVDAANSGSTCGTATNIAQATTITTGQSFTYAGFNVNEYGNPGGTTAGTAD
                     PSLNIAGCTDYLGQNAGNSANDITRLENTMWFKFTTPAIPCPSLSFYINNISCQNINT
                     SLNGGAGAQFILYKSNTCQTGANWNATKVYCQDKLTQSSPPIDLASMVLPNSTYYILI
                     DGFTGQHCNLDINLACPSLLPIELASFKATPDKDHIRLDWQVLSAANVEKYIIERSEN
                     GIDFSYITEKVAVSNHQNTINYTTDDYSAEKNNLYYYRLKTLDWDGKFSYSSVVSAIL
                     LEDGKMQISLFPNPASTNLYVRSLDTKSRTIPYIISDITGRIISSGELQTLKNVEQIR
                     MLDISQLEKGHYIFSCLNNEGIQAIQFVIE"
BASE COUNT          865 a          504 c          609 g          834 t
ORIGIN      
        1 tttgccacaa cagccattgc acatattggt attggcactt tgcctacctt atattttggt
       61 acagaagaac aaaaacaaaa atatctgccc aaactagcca gtggtgagtg ggcggcatca
      121 tactgcctga ctgagccaaa tgccggctct gatgctttgg gagcaaaaac aacggcaacc
      181 ttatctgaag acggcaaatc ctggatttta aacggacaaa aaatgtggat taccaatgcc
      241 ggttttgcag acctgtttac cgtttttgca aagattgacg gcaaagaatt tacaggtttt
      301 ctggtagaaa aaggaacgcc cggcctgagc ttgggtgcag aagaagataa actgggtatc
      361 aaaggttctt ctactcgcca ggtgtttttt gaaaattgca aaattccggt agaaaacatg
      421 ttgggcgaac gcggaaaagg acatttgatt gcatttaata tcttaaatat tggtcgatat
      481 aaattgggtg ctacagcttt aggcggggct cgcgaaacat ttaacgctgc tgttcgttat
      541 gccaatgagc gttaccagtt tggcaaacca attgcctctt tcggtgccat acaacataaa
      601 attgccgaca tggcaatcaa gatatttaca ctggaagtgt catcttaccg ctgtgccaat
      661 gatatcggta cgatggaaga agagctgaaa gtacaaggcg tagaatttgc aaaagcattg
      721 ttagagtctg ctgaagaata tgccattgag tgttctatcg taaaagtatt tggttctgaa
      781 gtactggatt ttgtagtgga tgaaaatgta cagatacatg gcggtatggg ttattctgaa
      841 gaaacagccg caccgcgtgc ttatcgtgat tcgcgcattg cccgtatcta tgaaggtacc
      901 aacgaaatca atcgtttgtt gtctatagat atgttgctga aacgtgctat gaaaggagag
      961 atagatttga tgaccccggg catggctatt caaaaagaac tgatgagtgt tcctgatttc
     1021 ggtagcgatg atgaaggtca atttgctgcg gaagaaaaag cattgaaaaa tgccaaaaaa
     1081 gcattcttgt tagtggcagg tggtgctgtg caaaaactga tggcaaaaat gaaagacgag
     1141 caagaaatca tcatgaactg tgcagatata ttaattgata tatacacggc agaatccttg
     1201 ttgttgcgag ttaaaaaaat agttgaaaaa ggagaaaaaa ccgatccggt ttatattgac
     1261 atcttaaaag tatattttaa tgatgccatg aaccgcatat ttgtaaatgg aaaagatgct
     1321 ttgcagtctt ttgccgaagg agacgaatta cgcattatgc aaatgggctt aaagcgattt
     1381 acaaaatacg aaccggtaaa tgtaaaagaa gcgcgcagac gtatagcagc aaaagtgctg
     1441 gaggccaaca aatacaactt gtaagaaagt aagataattc ctttaactac atacactctt
     1501 actgagtgta tgtagtaggt tttgtatatt ttgtcaagag atttactcaa ttacaaattg
     1561 aatggcttgt atgccctcat tattcagaca ggaaaatata taatgtcctt tttccagctg
     1621 actgatgtca agcatacgaa tttgctctac gtttttcaga gtttgcagtt caccgctaga
     1681 tattatcctt ccggtaatat cacttatgat atagggtatc gttctggact tagtatccaa
     1741 cgaacgtaca tacaaatttg ttgaggccgg gtttggaaat aaactgattt gcatttttcc
     1801 atcttccagt aatatagcag ataccacaga agaataagaa aacttgccgt cccaatccaa
     1861 cgtttttaaa cgataatagt acaagttgtt tttttctgca ctataatcat ctgttgtata
     1921 atttatcgta ttttgatgat tagatactgc cactttctcc gttatataac taaaatcaat
     1981 tccattttca ctgcgttcaa taatatattt ttctacattg gctgccgaca aaacctgcca
     2041 gtctaatcgt atatgatctt tatcgggcgt ggctttaaac gaagctaatt caataggcaa
     2101 aagactcggg caagccaaat taatatccag attacaatgc tgtcctgtga atccgtctat
     2161 taaaatataa taggtagaat tgggtagtac catgcttgcc aaatcaatcg gaggagaaga
     2221 ttgtgttagt ttatcctgac aataaacttt agtggcattc cagtttgcac ccgtttgaca
     2281 ggtattgctt ttgtatagaa taaattgcgc accggcaccg ccattcaacg atgtatttat
     2341 gttttggcag gatatattat taatataaaa tgaaagagag ggacaaggta ttgccggagt
     2401 ggtaaattta aaccacatcg tattttcaag acgcgtaata tcgtttgcac tgtttccggc
     2461 attttgtccc aaataatcgg tgcagcctgc aatgtttaat gaggggtctg ccgtgcctgc
     2521 cgtagttcct ccgggattac cgtattcgtt gacattaaat ccggcatagg taaaggattg
     2581 acctgtagta attgtagtag cttgtgcaat atttgtggct gtaccacacg tacttcctga
     2641 attggcagca tctactaagc gtttggcccc acagctgata tctgcccgat agccggcttt
     2701 ggttacagag ccgtctgaca taaatttaaa ggtaagacat cccgtggcat tattagttgt
     2761 tgcttttata atgcctaaag agtttagatc gtttggagaa aaatttgcca ag
//
LOCUS       DAOQII010000301         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_24968_length_2794_cov_5.510040, whole genome shotgun sequence.
ACCESSION   DAOQII010000301 DAOQII010000000
VERSION     DAOQII010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24968_length_2794_cov_5.510040"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLF48_11225"
     CDS             <1..961
                     /locus_tag="PLF48_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNF49946.1"
                     /translation="QKKVEENHFGTRKRLLEYDDVMNAQRDVIYKRRQHALIGERISF
                     DLINMFYDLSYEICNDAKISLDYEAFKLDCFRYLTVDTTISEADLKSLKTDDLAHHLY
                     TEVHEKYNRKSDDIIKHTYPIVKNVYESNSAQYKNIVIPFSDGNREINIIANLEKAYN
                     NKGRELIKVFEQNIILSFIDEYWKTHLRQMDELKQSVQMAVHEQKDPLLIFKFEAFEL
                     FKQMLSQINKEVTTFLMKGFLLTEDTQQIQQNRERKVVEKTRESRGNEQGQLGEEFKG
                     ASTGNETQVKQMPVRAEQKVGRNEPCPCGSGKKYKHCHGKDLA"
     gene            1018..1458
                     /locus_tag="PLF48_11230"
     CDS             1018..1458
                     /locus_tag="PLF48_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF49947.1"
                     /translation="MAFTHIKAQDYWNDSTDVENNILFFKDPRVDVLQKMYSYNKTVK
                     VVTEKLIRVQVMQGANRDEVFAAKTKFSQRFPGIPTYITYASPNFRLRAGEFASTQEA
                     QKFLQQLKSMFPASFMIEEKTSNDTDKDKNKQTGSKTKTPVKSK"
     gene            1495..2643
                     /locus_tag="PLF48_11235"
     CDS             1495..2643
                     /locus_tag="PLF48_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF49948.1"
                     /translation="MLSLVSLFTDMASEMLYPVMPVYLKKIGFSVLLIGILEGIAEAV
                     AGLSKSYFGKLSDVSGKRVPFVQFGYGLSAISKPLLATFIYPVWVFLCRTLDRVGKGI
                     RTGARDALLSDEATPETKATVFGFHRSMDTLGAVLGPALALVFLYYHPEQYKTLFLIA
                     FIPGVMAIAVTALLRESKKTKPNVTEQSVSFFTFINYWKDSPLLYKRLLLGLLLFALF
                     NSSDVFLLLKLKQEGMSDTTVIGVYIFYNLVYALLAFPVGILADILGLKRLYLSGLFA
                     FVFVYVGMSFNGSVYYYLLLFLLYGYYAAATEGIAKAWISNISEKKDTATAIGTYSGL
                     QSICALFASAFAGWLWFGFGAQATFITTGIMAIWVAIYLWKMPYKVIS"
BASE COUNT          904 a          471 c          583 g          836 t
ORIGIN      
        1 acagaaaaaa gtggaggaaa atcatttcgg caccagaaaa agattattgg aatatgatga
       61 tgtaatgaat gcccaacgtg atgtaattta taaacgtcgt cagcatgcat taatcggcga
      121 aagaatttct ttcgacctga taaatatgtt ttatgatttg agctatgaaa tttgtaacga
      181 tgcgaaaatt tctctggatt atgaagcatt taaactcgac tgtttccgtt atctgactgt
      241 tgacacgact attagtgaag cagatttgaa aagcttgaag acagatgatt tagcccatca
      301 cttatataca gaagtacacg aaaagtacaa ccgcaaatca gatgatatca tcaagcatac
      361 ttatcctatt gtgaagaatg tatatgaaag taattccgct cagtacaaaa atatagttat
      421 cccgtttagc gatggcaacc gtgaaatcaa tatcattgcc aatctcgaaa aagcatacaa
      481 caataaaggt cgtgaactga taaaagtttt tgaacaaaat atcatattat cctttataga
      541 tgaatactgg aaaacacatt tgcgccaaat ggatgaactg aaacaatcag tacaaatggc
      601 cgttcacgaa caaaaagacc ctcttttgat ttttaaattt gaggcttttg aattgtttaa
      661 acaaatgctg tctcagatta ataaagaagt gactactttc ttaatgaaag gattcttact
      721 gacagaagat actcagcaga ttcagcaaaa cagagaacga aaagtagtag agaaaacacg
      781 cgaatcaaga ggtaatgaac aagggcaact gggcgaagaa ttcaaaggtg caagcaccgg
      841 aaatgaaacg caagtgaagc aaatgccggt gcgtgctgaa caaaaagtgg gcaggaatga
      901 accttgtcct tgtggcagtg gtaaaaaata caaacattgc catggaaaag atttggctta
      961 aaatttgtta gttttgtatt gcttatgaaa aatgggattt tatttttagt actaattttg
     1021 gcttttacac atataaaggc tcaggattat tggaatgaca gtacggatgt ggaaaataat
     1081 atcttatttt tcaaagaccc gcgcgtagat gtattgcaaa agatgtactc gtataataaa
     1141 acggtgaaag tggtaactga gaaactgata cgtgtacaag tgatgcaagg ggcaaacagg
     1201 gatgaagtat ttgccgctaa aacgaaattt agccagcgtt ttccgggaat ccccacatat
     1261 atcacttatg cttctcctaa cttccgtctg cgtgcaggcg aatttgcatc gacacaggag
     1321 gcacaaaaat ttttacaaca actcaaatcc atgtttccgg ccagttttat gatagaagaa
     1381 aaaacaagca acgacacgga taaagataaa aacaagcaaa caggcagtaa aacaaagaca
     1441 cctgttaaat caaaataatt tgtaaagctg aaaacgctta cccgcactat ttggatactt
     1501 tcgttagtca gtctttttac agatatggcc agtgaaatgt tatatccggt gatgcctgtt
     1561 tatctcaaaa aaataggatt ttctgtcttg ctgattggta ttctggaagg aatagcagaa
     1621 gcagtggcag gcttgagcaa gagctatttc ggcaaacttt ccgatgtatc gggtaagcgc
     1681 gttccttttg ttcaattcgg atatggactt agtgccattt caaaaccctt gctcgccact
     1741 tttatttacc ccgtttgggt atttttatgc cgcacactgg atcgggtagg aaaggggata
     1801 cgcaccggag caagagatgc gctattgtca gatgaagcca cacccgaaac taaggctacc
     1861 gtattcgggt ttcatcgttc tatggatacg ctgggtgcag tattgggacc tgcactggcg
     1921 ctggtttttt tatactatca ccccgagcaa tataaaacac tgtttttaat cgcatttatt
     1981 ccgggtgtaa tggctattgc agtaactgct ttgctgcggg aaagcaaaaa aactaagcca
     2041 aatgtaacag agcagagtgt ttcatttttc acattcatca attactggaa agatagtcct
     2101 ctgctttaca aacggctgct tttgggcttg ctactgtttg ctttgtttaa cagctctgat
     2161 gtgtttttgt tattgaaatt aaaacaagag ggaatgagtg atactacagt aatcggagtc
     2221 tatatttttt ataatttagt ttatgcttta ctggctttcc cagttggtat tttagcagat
     2281 atattaggat tgaaacggct ttatttatcc ggtctgtttg cattcgtttt cgtatatgtt
     2341 ggtatgtcgt ttaatggttc ggtgtactat tatttacttt tgtttttact ctatggctat
     2401 tacgcagcgg caactgaagg gatagccaaa gcctggatta gtaatatttc tgaaaaaaaa
     2461 gatactgcta ctgcaattgg cacctacagc ggattgcaaa gcatttgcgc cttgtttgcc
     2521 agtgcttttg ccggatggtt atggttcggt ttcggagcac aggcaacatt tataactacg
     2581 ggcattatgg ctatctgggt tgcaatttat ttatggaaga tgccatataa agtcatctcc
     2641 taatggcaaa ttttaaatgt gataacctga gtttgggata agaaaaatag agtaaaaaac
     2701 agttgatttc tcatttttcc tccaacttaa caaagaggtg gaataaaaag gtggtggttc
     2761 ttaataaaaa aattaactat tcttatgttg aagc
//
LOCUS       DAOQII010000302         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_24970_length_2794_cov_4.510770, whole genome shotgun sequence.
ACCESSION   DAOQII010000302 DAOQII010000000
VERSION     DAOQII010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24970_length_2794_cov_4.510770"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1472)
                     /gene="nrfD"
                     /locus_tag="PLF48_11240"
     CDS             complement(33..1472)
                     /gene="nrfD"
                     /locus_tag="PLF48_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNF49949.1"
                     /translation="MASHFESPIREPLIRGHKTYKDITNDIVRPITGKIQPRWAFWTA
                     VSAVVASFGVFCLVWEVCFGIGTWGANKTVGWAWDITNFVWWIGIGHAGTLISAILLL
                     FRQKWRTGVNRAAEAMTIFAVICAGLFPVFHMGRVWNAFFIFPYPNTRGPLWVNFNSP
                     LLWDVFAISTYFTVSLLFWYTGLVPDFATVRDQSKTTGRWKFYNFLSFGWVGSAKHWE
                     RWESLSLILAGLSTPLVLSVHTIVSFDFSTSVVPGWHTTIMPPYFVAGAIFSGFAMVL
                     TLMLVARYIMRLEDYITLAHIESMNKILLLTGTMVGIAYLTEIFIAWYSGSMYEQYAF
                     ANRAMGPYWWSYFGMMFCNLVSPQVFWFRSLRRNIKVTFIMSIFVNIGMWFERFVIIV
                     TSLHRDFIPSSWVMYSPTWVEMGIYIGTMGIFMTLFLLFCRFFPAIAIAEVKSILKIS
                     GDQYREKQLEEVKNMEAYKGVSVSEHAWD"
     gene            complement(1504..>2794)
                     /locus_tag="PLF48_11245"
     CDS             complement(1504..>2794)
                     /locus_tag="PLF48_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNF49950.1"
                     /translation="FSDEEIMPSDKRVKTANVTINGKTYKDLPVVVQFGQAPNTIAVA
                     LGYGREKAGKAGDGVGKNVYGAVAYVNGIASYSSADAKIEFNSSKYELALNQTYHTLM
                     EDNNLPLRPKRFRHGILKDTNLEEYKKNPHAGNEDREKIKEEFSNSLYDAYTPNDYKQ
                     GHHWGMTVDLNSCIGCGSCVVACSVENNVPVVGKRQVFRSQEMHWMRIDRYYSGNPDN
                     PDVAFQPMMCQHCNNAPCENVCPVNATNHSIDGINQMAYNRCIGTRYCANNCPFKVRR
                     FNWLDYQAADVFGKWNDGKRSFTDTEAQSYMFEDLTRMVLNPDVVVRSRGVIEKCSFC
                     IQRIQEGKLNAKKENRQVKDGEIKTACQTACPTNAITFGDQNDKEGAVAKTFNESGRN
                     YFVLEEYHVVPAVGYQVKVRNIDEKPFDYFIYKPGLA"
BASE COUNT          875 a          617 c          469 g          833 t
ORIGIN      
        1 catagtatta taatatactg tgtttttaaa aattaatccc aagcatgttc actcacactc
       61 acacctttat atgcttccat atttttcacc tcttccagtt gtttttctct gtattgatct
      121 cccgatattt tgaggataga ttttacctca gcaatagcga tagccgggaa gaagcgacag
      181 aataaaagga ataaagtcat aaagatgccc atggtaccga tatagattcc catctccacc
      241 catgttggtg aatacatcac ccagcttgac ggaataaaat cacggtgcaa agaggttacg
      301 ataattacaa aacgttcgaa ccacataccg atatttacaa agatggacat gataaatgta
      361 actttaatat ttcttctcag gcttctgaac cagaacacct gcggagagac cagattacag
      421 aacatcatac caaaatacga ccaccagtaa ggtcccattg ctctgttagc aaatgcatat
      481 tgctcataca tgctaccgga ataccatgca ataaatattt ctgtcaaata ggctatacct
      541 accatagttc ccgtcagcaa caagatttta ttcatcgact caatatgcgc cagtgtaata
      601 taatcttcaa gtcgcataat gtaacgagct accaacataa gcgttaatac catggcgaaa
      661 ccggagaaaa ttgcaccggc aacgaaataa ggtggcataa ttgtagtatg ccatcccgga
      721 actactgagg tggagaagtc aaaagatacg atagtgtgta cagaaagtac aagtggcgta
      781 gataaacccg ctaatattaa tgaaagtgat tcccatcttt cccaatgttt ggcagaacca
      841 acccatccga atgataagaa attatagaat ttccagcgac cagttgtttt agactgatca
      901 cgaactgttg caaaatccgg cactaaacct gtataccaaa ataataagga aaccgtgaaa
      961 tacgttgaaa ttgcgaatac gtcccaaagc aaaggagaat taaagttaac ccaaagtggc
     1021 cctcttgtat taggataagg gaagatgaag aatgcattcc atacacgacc catgtgaaaa
     1081 accggaaata agcccgcaca aataacggca aaaattgtca tcgcctcagc agcacggttt
     1141 acacctgtac gccatttttg acggaataac aataagattg cagaaatcag cgttccggca
     1201 tgaccgatac caatccacca aacgaagtta gtaatgtccc atgcccaacc aactgtctta
     1261 ttcgcccccc aagttccaat accaaaacat acctcccaca ctaagcaaaa tacgccaaat
     1321 gacgctacaa ctgcagaaac tgctgtccaa aatgcccaac gaggttggat tttacctgtg
     1381 ataggacgta caatgtcatt agtaatgtct ttgtaggtct tatgccccct gattaacggt
     1441 tctcgaattg gtgattcaaa atgacttgcc attataatat ctgtttcgac ttttaataat
     1501 taattatgct aaacccggtt tatatataaa ataatcaaac ggtttttcat caatatttct
     1561 cacttttacc tgataaccaa ctgcaggaac tacgtgatat tcttccaata caaaataatt
     1621 tctgccactt tcattgaaag tttttgccac tgctccttct ttatcatttt ggtcgccaaa
     1681 agtgattgca ttagtaggac acgctgtttg acatgctgtt tttatttctc cgtcttttac
     1741 ttgacggttt tcttttttag cattcagctt accttcctga atacgttgaa tacagaatga
     1801 acatttttca ataacccctc ttgaacgaac tactacatcc ggatttaaca ccatgcgggt
     1861 taaatcttca aacatataag attgagcttc tgtatctgta aaagagcgtt taccgtcatt
     1921 ccatttgcca aatacgtctg ctgcctgata gtctaaccag ttgaaacgtc gtactttgaa
     1981 cggacagtta ttcgcacaat atctcgttcc gatacaacgg ttatatgcca tttgattaat
     2041 accatctata ctgtggttgg ttgcatttac tggacatacg ttttcacaag gtgcgttgtt
     2101 acaatgttgg cacatcatag gctggaatgc cacatccgga ttgtccggat taccactata
     2161 ataacggtca atacgcatcc agtgcatttc ctgagagcgg aatacttgtc gttttccaac
     2221 taccggaaca ttattttcga cactacaagc caccacacaa gaaccacaac caatacaaga
     2281 gtttaagtca accgtcatac cccaatgatg accttgttta tagtcatttg gtgtatatgc
     2341 atcatataat gaattactga attcttcttt tattttttct ctgtcttcat tacctgcatg
     2401 cggatttttc ttatactctt ccaaattcgt atctttcaag ataccatgtc tgaaacgttt
     2461 aggtctgagt ggaagattat tatcttccat cagtgtatga taagtttggt tgagcgctaa
     2521 ttcatactta gaagaattga actcaatttt tgcatcagct gaagagtaag atgcaatgcc
     2581 atttacataa gcaacagcgc cataaacatt ttttcccaca ccgtctccgg ctttgcctgc
     2641 tttttctcta ccgtatccca atgcaactgc aattgtattg ggagcttgtc cgaattgaac
     2701 aacaaccggc aaatctttat acgttttacc attaattgtt acattagcag tttttactct
     2761 tttgtcagaa ggcatgattt cttcatcaga aaat
//
LOCUS       DAOQII010000303         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_25012_length_2792_cov_3.480818, whole genome shotgun sequence.
ACCESSION   DAOQII010000303 DAOQII010000000
VERSION     DAOQII010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25012_length_2792_cov_3.480818"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLF48_11250"
     CDS             <1..323
                     /locus_tag="PLF48_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF49951.1"
                     /translation="LVGNYNGGLWYYSEKIPTVTAVKPAKTQTLSFNIFPNPAGNNMT
                     LEFDNKVLPAQGAVYSIDGKNVLSFEVKSINTQLNIADLPKGIYLISVYNQQYTGTSK
                     FVKE"
     gene            415..912
                     /locus_tag="PLF48_11255"
     CDS             415..912
                     /locus_tag="PLF48_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNF49952.1"
                     /translation="MNYLILALSALVPMALGFVWYGPLFGKAWIKETGLSEDELKANF
                     NPIKVFGLALLFSFMIAFMMEPLVIHQYSLFQLTMHFKNDPATKATLAELMTRYGNEF
                     RTFHHGALHGALSGLFLIFPVLSINALFERKSWKYIFINSGFWIISFALMGGIICQFI
                     KFDGF"
     gene            930..1538
                     /locus_tag="PLF48_11260"
     CDS             930..1538
                     /locus_tag="PLF48_11260"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNF49953.1"
                     /translation="MYLELYPDNIDMRKMKQIVDCLRSGGIIIYPTDTVYGLGCDIYN
                     RDAIEKVCRLKKVKPEKMNFSFVCYDLSHISEFTLNLDTPTYKLMRRCLPGPFTFILN
                     ANNSIPKLFKNNKRTIGIRVPDNNIARTIVHELGNPILSTSIHHEDDIIDYITEAYSI
                     YERYEHTVDIVIDGGTGGIIPSTVIDCTHDEPVIIREGLGVL"
     gene            1590..>2792
                     /gene="hutI"
                     /locus_tag="PLF48_11265"
     CDS             1590..>2792
                     /gene="hutI"
                     /locus_tag="PLF48_11265"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNF49954.1"
                     /translation="MYLINIGFLVNVREQITLLRGADLAILPVLENAYLKIQDDCIVD
                     YGLMQDINPDEIKDELYDVKGASVLPSWCDSHTHLVFAAYRESEFIDKIKGLSYAEIA
                     AKGGGILNSAQKLQNTPEEILLEQTQQRLDELIQLGTGAIEIKSGYGLSVEGELKMLR
                     VIQQLKKQNSIPIKSTFLGAHAIPASFKDNKAGYISILTDELLPKIAADNLADYIDVF
                     CENGFFTQAETEYICMKGMDYGLRAKIHANQLNRSGGVQAGVNVNALSVDHLETIGED
                     EIALLSYENAPVATLLPSCSFYLRMHYAPARTLLEHNAAIALASDFNPGSSPSGNMNF
                     VLSLACIQMKMLPEEAINAATVNGVFAMEVQDLVGSITIGKKANLIITKHISSLAFLP
                     YAFGNNCID"
BASE COUNT          887 a          486 c          522 g          897 t
ORIGIN      
        1 tattagttgg taattacaat ggaggcttat ggtattattc tgaaaaaatc ccgacagtta
       61 ctgctgtaaa acctgctaaa actcagacgt tatccttcaa tatttttcct aatcctgccg
      121 gcaataacat gacacttgaa tttgataata aagtattgcc agctcaaggc gcagtttatt
      181 ccattgatgg taaaaatgtc ttgtcgtttg aagttaaaag tataaatacg caacttaata
      241 ttgccgactt gcctaagggt atttatttaa tttctgttta caatcaacaa tataccggaa
      301 caagcaaatt tgtaaaagaa taaggtcatc aagtagtttt tatctgattt ttatacagaa
      361 acttttttac actgaatcat caaatttgat gtatttttac ataaaataca caatatgaac
      421 tatttaattt tagcactttc ggcattagtg ccaatggcat tgggctttgt atggtatggc
      481 ccgcttttcg gcaaagcttg gataaaggaa actggcttat ctgaagatga acttaaggca
      541 aatttcaatc ccataaaagt atttggctta gccttacttt tttcatttat gattgcattt
      601 atgatggagc ctttggtcat tcatcagtat tcgctgtttc aactcaccat gcatttcaaa
      661 aatgatcctg ctactaaagc aacacttgca gaattgatga ccagatacgg aaatgagttt
      721 cgtacatttc atcatggtgc cctacatggt gcgttatcag gcttattttt aatatttcct
      781 gttttaagca taaatgcctt atttgaaaga aaaagctgga aatatatttt tatcaattca
      841 ggtttttgga taatatcatt tgctttgatg ggtggaatca tctgtcagtt cataaagttt
      901 gacggatttt agtcgtacct ttgcgcatca tgtatctcga attatatccg gataatattg
      961 acatgcgcaa aatgaaacaa attgtagatt gcctgcgcag tggaggtata atcatttatc
     1021 ctaccgatac agtgtacggc ttaggttgtg atatttataa cagagatgca attgagaaag
     1081 tctgtcggct gaaaaaagtg aaacctgaaa aaatgaactt ttcttttgtc tgttatgact
     1141 taagccatat ctccgaattt actttaaatc tggacactcc tacttataaa ttgatgcggc
     1201 gatgcttacc gggaccattt acatttatct taaacgcaaa taattccata cccaaattat
     1261 ttaaaaataa caaaagaact atcggtattc gtgttcctga taataatatt gcccgcacaa
     1321 ttgtacacga attgggcaac ccgattttgt ctacttccat ccaccatgaa gatgatatca
     1381 ttgattatat cacggaagcc tattcaattt atgaacgcta cgaacatact gtagatatcg
     1441 tcattgatgg cggaactggt ggcattatac catctacggt tatagactgc acacacgacg
     1501 agccggtaat tataagagaa ggattaggcg ttttgtgaaa taatatgcaa tccttgactt
     1561 acaactaatt ttcgtaattt gtgcctgtca tgtatcttat caatattggc tttttagtaa
     1621 atgttagaga gcaaattact ttgctgagag gtgcagattt agccattttg cctgtcttgg
     1681 aaaatgctta tctgaaaatc caggatgact gcattgttga ttatggtttg atgcaggata
     1741 ttaatccgga tgaaattaag gatgaattgt atgatgtaaa aggtgcttca gtattgccta
     1801 gctggtgcga tagccatacg catcttgttt ttgcagcata cagagaatca gaatttatag
     1861 ataaaataaa aggcttatcc tatgctgaaa ttgccgcaaa gggtggcggt attttaaatt
     1921 cagcacaaaa actacaaaac acacccgaag aaattttact cgaacaaacg cagcaacgtc
     1981 tcgacgaact gatacaattg gggactggag caatagagat aaaaagcggg tatggtttga
     2041 gtgtagaagg cgaattaaaa atgctgcgtg ttattcagca actgaaaaaa caaaattcca
     2101 tccccataaa atctactttt ttgggcgcac atgccatacc ggcttctttt aaagataata
     2161 aagcaggcta tatttcaata ttgacagatg aattgttacc caaaattgct gcggataatt
     2221 tagctgatta tatcgatgtg ttttgtgaaa acggattttt tactcaagca gaaacagaat
     2281 atatctgcat gaaaggaatg gattatgggt tgcgggcaaa aatacatgcc aatcagctta
     2341 accgttccgg tggagtgcaa gcaggtgtaa atgtaaatgc attgtctgta gatcatttag
     2401 aaacaatagg tgaggacgaa attgccttat tatcttacga aaatgcacct gttgccacac
     2461 ttttgccatc atgttccttt tatttgcgca tgcactacgc accagccaga acactgttag
     2521 aacacaatgc tgctattgca ctggcaagcg attttaatcc cggctcttca ccaagtggaa
     2581 atatgaattt tgttttgtct ttagcatgca tacaaatgaa aatgctcccc gaagaagcta
     2641 tcaatgcagc tactgtcaac ggtgtttttg ctatggaagt acaagattta gtcggttcaa
     2701 ttactatagg gaaaaaagcc aatctcatta ttacaaaaca tatttcatca cttgcatttt
     2761 tgccatatgc atttggcaat aattgcatag at
//
LOCUS       DAOQII010000304         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_25069_length_2788_cov_6.105013, whole genome shotgun sequence.
ACCESSION   DAOQII010000304 DAOQII010000000
VERSION     DAOQII010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25069_length_2788_cov_6.105013"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1484
                     /locus_tag="PLF48_11270"
     CDS             225..1484
                     /locus_tag="PLF48_11270"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNF49955.1"
                     /translation="MAEENVLIAYNGKSSNCAVFIGTENEPSIDISNLREETGLVTLD
                     FGYKNTGATKSAITFLDGELGILRYRGYEIADLAEKSNFTEVAYLLLYGELPTLVQLT
                     AFNGKLQENSDVPLHIFELLKSMPKGTHPMAQLSAATVALSATFGKDATKVTEETIIN
                     ILAKFPVIVGMIMRNYQGLDFVENDKSLDYVSNFMQMAFGKKASSVALDAMDKLLILH
                     ADHEQNCSTSTVRMVGSANSNIYASVAGGINALWGSAHGGANQAVLEQLEAIENDGGD
                     TAKFVAMAKDKASGFRLMGFGHRVYKNFDPRANIIKKSCDEVLNDLGVVSKKLTIAKE
                     LEQAALHDEYFVSRKLYPNVDFYSGIIYNALDFPTDMFTALFALGRLPGWVSQWKEMK
                     ENKEPIGRPRQIYTGATIRPYPEIENR"
     gene            1484..1858
                     /locus_tag="PLF48_11275"
     CDS             1484..1858
                     /locus_tag="PLF48_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49956.1"
                     /translation="MKVFSAIVLCGLMLLSSSPHLTYLLIYKSFQKKITRDFCINKNK
                     PNSCCEGSCHLAKVEKKSEDNPVAPFSSAFKFREVEWVKQKLCLVVLQLPQSAALQQN
                     TCIYNAPVFSGYTTTLFHPPCC"
     gene            1897..>2788
                     /locus_tag="PLF48_11280"
     CDS             1897..>2788
                     /locus_tag="PLF48_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNF49957.1"
                     /translation="MKKTISFLYGLLLLAKVNACDLCGCNPGVFSGDFLSSEPQSYAA
                     LSSSYKQYKMLDSSSAVERIHLFNQTLTIGYSPKSWVELRLMLPYSLMVNQYAADKKQ
                     SVGLNDITLFSNFRIINKPACNTKHQFGHQLNIGAGVEFPTGKNIANTMDELQNFTFG
                     SRSFDVPMSLTYLLSYKNWSLIQFVYMKFNTVNKEKVRYGSMYTYQLSASYQHSFKKI
                     AFLPNIGLRSEFQDRNLRKNIIQHFSGTKLLLLTAGFDIKWKMLSGGLSLQQPLWQNT
                     ANFTIKQKSTITLNLRYQFPK"
BASE COUNT          881 a          513 c          541 g          853 t
ORIGIN      
        1 agtatttttt ttaaatttaa atattataaa taaattgcat attttctttt cgacactttg
       61 ttttattaaa acagatgcaa tacattttca tcccgattta cttgtttgat tttaattatt
      121 caggatgctc caattactag tttgggtttt gacacaatcg gaataatatt atcttaatgt
      181 agtatggcca atctttttgt aactttagct tcacatttta tgttatggca gaagaaaacg
      241 tgcttatcgc ttataacggc aaatcctcaa actgtgccgt atttatcgga accgaaaacg
      301 aacccagcat tgatatttca aaccttagag aagaaacagg gctggtaacc ttagattttg
      361 gatataaaaa cacgggagca accaagagcg ccatcacttt cttggatggt gaactgggta
      421 ttttgcgcta ccggggttat gaaattgccg acttggcaga aaaatcaaat tttacagaag
      481 tcgcatattt gttgctgtat ggcgaattgc caactttagt gcaattaaca gcattcaatg
      541 gaaagctaca ggaaaattct gatgtgcctt tgcatatttt tgaattatta aaatcaatgc
      601 cgaaaggcac acaccccatg gcacagctga gtgctgcaac ggtagccctg tctgcaacct
      661 ttggtaaaga tgccactaaa gtaacggaag aaacaattat caatattctg gctaagtttc
      721 cggtaattgt tggcatgatt atgcgcaact accaagggtt ggattttgta gagaatgaca
      781 aatcattgga ttatgtatcc aatttcatgc aaatggcatt tggcaaaaaa gcaagttctg
      841 tagctttgga tgctatggat aaattattga ttttgcatgc ggatcacgaa caaaactgtt
      901 ccacatcaac ggtacgtatg gtgggttctg ccaattccaa tatttatgca tctgtagccg
      961 gaggcatcaa tgccctttgg ggttctgcac atggtggagc caatcaggca gtactggaac
     1021 aactggaagc tatcgaaaat gatggtggag acaccgctaa atttgtagcc atggctaaag
     1081 acaaagcttc cggcttcaga ttgatgggct tcggacaccg cgtgtataaa aactttgacc
     1141 ctagagcgaa tattatcaaa aaatcctgtg atgaggtttt aaatgatttg ggtgtggtta
     1201 gcaagaaatt gactatcgca aaagaattag aacaagccgc actgcatgat gaatattttg
     1261 taagcagaaa actctatcca aacgttgact tttattcagg tattatttat aatgctctgg
     1321 attttcctac agatatgttt actgctttat ttgccttggg cagattaccg ggttgggtgt
     1381 cgcagtggaa agagatgaaa gaaaataaag aaccaattgg cagaccgcgt caaatttata
     1441 ccggggcgac tattagacca tatccggaaa tagaaaacag ataatgaaag tattttcagc
     1501 aattgtatta tgtggcttga tgttactcag cagttcacca catttgacgt atttgctgat
     1561 ttacaaatca ttccagaaaa aaatcacccg agatttttgt atcaacaaga acaagccaaa
     1621 cagttgttgt gagggcagct gtcacttagc aaaagttgaa aaaaaatcgg aagacaatcc
     1681 tgttgcgcca ttctcttctg cattcaaatt cagagaagta gagtgggtaa aacagaagtt
     1741 atgtcttgtt gtacttcaat tacctcaatc tgctgcacta cagcaaaata catgcatata
     1801 caatgcccct gtttttagcg ggtataccac aaccttattt cacccacctt gttgctaggt
     1861 taatcttatt tttttaacct tttcaacaac atttccatga aaaagactat ttctttttta
     1921 tatggtttgt tgctgttggc aaaggtaaat gcctgtgatt tatgcggatg taatccggga
     1981 gttttctccg gcgattttct ttcttccgag ccacaatcat atgcagccct ttcgagcagt
     2041 tacaagcaat ataaaatgct ggattcttca tcagcagtag aacgaattca cctttttaat
     2101 caaacactaa cgataggata ttcacccaaa agctgggtag aattgcgatt gatgctgccc
     2161 tattcgctta tggttaatca atatgccgca gacaaaaagc aatctgtcgg gttaaatgat
     2221 attaccctct ttagtaattt cagaattatc aataagccgg catgcaatac aaaacatcaa
     2281 ttcggacatc aattaaacat cggtgccggg gttgaatttc ctacagggaa gaatattgcc
     2341 aacaccatgg acgaattaca gaatttcaca tttggttcca gatcgtttga tgttcccatg
     2401 tcattaactt atttattaag ttataaaaac tggagtttga ttcagtttgt atatatgaaa
     2461 ttcaacacgg ttaataaaga gaaagtgagg tatggcagta tgtacactta tcagctatct
     2521 gcaagttacc agcatagttt taagaaaata gccttcttgc ccaatatcgg gttgcgttct
     2581 gagtttcagg acagaaacct gcgcaaaaat atcatacagc atttttcagg tacaaaacta
     2641 ttattactta ctgctggttt cgatataaaa tggaaaatgc tcagcggagg cttgtcatta
     2701 caacaaccgc tttggcaaaa cacggctaat ttcactatca aacaaaagag tacaattacg
     2761 ctaaatctga ggtatcaatt tcccaaaa
//
LOCUS       DAOQII010000305         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_25107_length_2786_cov_4.944343, whole genome shotgun sequence.
ACCESSION   DAOQII010000305 DAOQII010000000
VERSION     DAOQII010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25107_length_2786_cov_4.944343"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /gene="rpoC"
                     /locus_tag="PLF48_11285"
     CDS             complement(<1..2177)
                     /gene="rpoC"
                     /locus_tag="PLF48_11285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNF49958.1"
                     /translation="MPFKKDNKIKTEFSTITLKLASPDSILERSFGEVKKPETINYRT
                     YKPEMEGLFCERIFGPTKDYECFCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYILGLSSKNVETIVYYERYAVIQPGITEDGVTVNGEKKT
                     TKYLDLITEEEYLDILDALPKDNQLLSDDDPNKFIAKMGGEALRDLLSRIELTKLSAE
                     LRHKASNETSQQRKSEALKRLSVVEAFREANEHVENRPEWMIVQYLPVVPPELRPLVP
                     LDGGRFASSDLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRK
                     SNAVKAEGGRALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPK
                     DMAAELFKPFIIRKLLERGIVKTVKSAKKLVDKKEAVVWDILENVMKGHPVMLNRAPT
                     LHRLSIQAFQPKLIEGKAIQLHPLVTTAFNADFDGDQMAVHVPLSNAAILEAQLLMLA
                     SHNILNPQNGTPITIPSQDMVLGLYYLTKGKKTMGDDKVKGEGKVFYSAEEAIIALNE
                     GQVDLHAYVKCRVRVLENGKPAMKLIETTVGRLIFNEVVPPQCSYVNELLSKKSLKNV
                     IGDVLASTDIPTTVKFLDDIKELGYRQAFKGGLSFNIDDLIIPNIKSKLLTDASEEVD
                     EVWANYNMGLITNNERYNQIIDIWTRVDTRITDSLMKEISTHR"
BASE COUNT          873 a          541 c          461 g          911 t
ORIGIN      
        1 tgacggtgag tagaaatttc tttcatcaaa ctatccgtga tacgagtgtc tacacgtgtc
       61 cagatgtcaa taatctggtt ataacgttcg ttgttggtaa ttaaacccat attatagtta
      121 gcccaaactt catccacttc ttcagaagca tcagtcaaca atttagattt gatattcggt
      181 ataatcaaat catcaatatt aaatgataat cctcctttga atgcctggcg gtaacctaat
      241 tctttgatat catcaaggaa tttaaccgtc gtagggatat cagtactggc taatacatca
      301 ccaattacat tttttaaact ctttttagag agtaactcat ttacataaga acattgagga
      361 ggtactactt cattaaatat tagacgacct accgtagttt ctatcagttt cattgccggt
      421 ttgccatttt ccagcactct tacacggcat ttaacatatg catgcaaatc tacctgtcct
      481 tcatttaaag cgataatagc ttcttctgca gaataaaata ccttgccttc tcctttcact
      541 ttatcatctc ccatggtttt cttacctttt gtaagataat acagacccaa taccatgtct
      601 tgagacggaa tcgtaattgg tgtaccgttt tgagggttca ggatattgtg cgaagccaac
      661 attaaaagtt gagcttccaa aatcgcagca ttgctcaaag gcacatgcac cgccatttgg
      721 tcaccgtcaa agtcggcgtt gaatgccgtg gttaccaatg ggtgaagctg aatggcttta
      781 ccttcaatta atttaggctg gaaagcctga atagacaagc ggtgtaatgt cggggcacgg
      841 tttagcataa ccggatgtcc tttcattaca ttttccaaaa tatcccaaac aactgcttct
      901 tttttatcta ctaatttttt agcagatttt acagttttta ctatacctct ttcgagtaat
      961 tttctgataa taaacggctt gaataactcg gcagccatat ctttaggcaa accacactcg
     1021 tgcaatttca attcaggacc aaccacaatt accgaacgac cggaatagtc aacacgttta
     1081 cccaacaaat tctgacggaa acgaccttgt tttcctttta aaacatcaga aagtgatttc
     1141 aaagcacgac caccttctgc ttttactgca ttggatttcc gtgagttatc aaacaaagag
     1201 tcaactgctt cttgaagcat acgtttttca tttcgtaaga taacttcagg agcttttatt
     1261 tcgataagac gtttcaaacg attgtttctg ataatgacac gacgatataa atcgttcaaa
     1321 tctgaagaag cgaaacgacc tccatctaat ggaactaatg gacgcaattc aggtggaaca
     1381 actggaagat attgtactat catccattcc ggacgatttt ccacatgctc gttggcttca
     1441 cggaaagctt ccaccacgct caaacgtttc aaagcttctg acttacgttg ttgagaagtt
     1501 tcatttgatg ctttatggcg caattctgca gacaatttgg tcaattctat tcttgataac
     1561 aggtcacgca gagcttcgcc acccattttt gcaataaact tatttggatc atcatcagac
     1621 aaaagttgat tgtcttttgg caatgcatct aaaatatcca gatattcttc ttctgtaatt
     1681 aaatccagat atttcgttgt tttcttttca ccgtttacag taacaccgtc ttcagtaatg
     1741 cctggctgaa taacagcgta tctctcgtaa taaacgatag tttccacatt tttagaagac
     1801 aagcctaaaa tataacctat tttattaggt aaagatttga aataccaaat atgaacaacc
     1861 ggaactacta atttgatatg tcccattcgc tcacggcgaa ctttcttttc tgtaacttct
     1921 acaccgcaac ggtcacacac aattcctttg tagcggattc ttttatattt gccgcaaaaa
     1981 cattcataat cttttgttgg cccaaaaatg cgctcacaaa acagaccttc catttcgggt
     2041 ttgtaggtac ggtagttgat agtttcgggt ttttttactt cgccgaaaga acgctcaagg
     2101 atagaatcgg gagaagccaa ttttaacgtg atagttgaaa attcggtttt gattttgttg
     2161 tccttcttga aaggcataag ctgttgattt ttagttagat ataataaaaa aaacggatta
     2221 agtacaccta cttatccaaa atcgaactgc gaatatacga ttattcctcc aaatttagaa
     2281 ttccgtaaag aataaaataa cattaattta cagaattcgg agaatgcatt ataataatta
     2341 aaatcttgtt ccaaatttga aacacagagc atgtatagct atttataaat atagttttta
     2401 tctcatgtga agtataattt ataattttta ccacctggat gctttattag ctttggaata
     2461 acaatgctac agcacaaaaa atgaaataag aaccaactcg ttaagcttat tatgatttaa
     2521 tgttgaacat ccccaaatag ttgatttagt tttttagaac caccaccttt taattcctcc
     2581 tccttgttaa gttggagaaa aaatgagaaa tcaactattt tatttcattg agttgcttga
     2641 accgaactta attagaagag tgttctgtat ttaggaaaat ggataaaaat catttttttt
     2701 gtcggtattg ggcaaatgaa aacgaataag ttgataaatg gaatggctta cgaaaaatag
     2761 caacaatatt aaatgcgaaa gtgcga
//
LOCUS       DAOQII010000306         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_25578_length_2757_cov_3.442265, whole genome shotgun sequence.
ACCESSION   DAOQII010000306 DAOQII010000000
VERSION     DAOQII010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25578_length_2757_cov_3.442265"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2535)
                     /locus_tag="PLF48_11290"
     CDS             complement(97..2535)
                     /locus_tag="PLF48_11290"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNF49959.1"
                     /translation="MNPLFFVVPAMGLFGLLITFVKFNWVKKQDAGDANMKELAGYIA
                     DGAIAFLKAEWKILTYFGIIAAVLLAVLGFAGGEGSSPVIALAFLTGAIFSATAGYIG
                     MKVATLANVRTTQAARTSLPQALKVSFTGGSVMGLGVAGLAVLGLGSLFIMFYFLFVH
                     TNNGTPEWAEQVKRAIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGI
                     PEDDVRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGQETISNDKFGGLAP
                     ILLPMAIAGTGILFSWIGTFFVTISNENDNVQGALNKGNWGSIILTAIASIGLVKFIL
                     PTEMVLRGHTFTSNGVLGAIFVGLVVGALMSIITEYYTAMGKRPVLSIVRQSSTGHAT
                     NVIGGLAVGMESTLLPIIVLAAGIFGSYMCAGLYGVAIAAAGMMATTAMQLAIDAFGP
                     IADNAGGIAEMSQLPEEVRGRTDILDAVGNTTAATGKGFAIASAALTSLALFAAFVGV
                     AKIDAIDIYKAPVLAGLFIGGMIPFIFSSLAISAVGRAAMDMVNEVRRQFREIPGIME
                     YKAKPEYEKCVQISTQASIREMMLPGAIALITPVVIGFLFGPEVLGGVLAGVTVSGVL
                     MGMFQSNAGGAWDNAKKSFEKGVDINGETFYKKSEPHKAAVTGDTVGDPFKDTSGPSM
                     NILIKLMSIVSLVIAPYITIGGLLHVDNDAKAAACCPEMSKEACMGMSPEECAAKMGN
                     MDSTFTFVNENGDTVVKHIVIKKDFACNEEDCKKAGCENPGKCDPSSCDKPCCADKKH
                     CTNEEMKKDCCMKK"
BASE COUNT          839 a          669 c          477 g          772 t
ORIGIN      
        1 gtataaatgt taaaaacgat taataagttc aattattgtg cctaaaataa aaaatcccga
       61 caagttgtcg ggattcattt tatatttagt attgccttat ttcttcatgc agcaatcttt
      121 cttcatttct tcattcgtgc agtgtttttt gtctgcgcaa cagggcttat cacagcttga
      181 aggatcacat ttccccggat tctcacaacc tgctttttta caatcttctt cattacatgc
      241 aaaatctttt tttatcacga tatgcttaac aacggtatcg ccgttttcat ttacaaatgt
      301 aaacgtagaa tccatattac ccatcttggc cgcacattct tcaggactca ttcccataca
      361 agcttctttg ctcatttcag ggcaacaagc tgcagcttta gcatcattgt caacatgcaa
      421 caatccgcca atggtaatat aaggagcgat tactaatgaa acgatagaca tcaatttaat
      481 caggatattc attgacggac ctgaggtgtc tttaaacgga tcgcctacgg tatcaccggt
      541 tacggctgct ttatgcggct ctgatttttt ataaaaagtt tctccgttga tatccacgcc
      601 cttttcaaat gatttttttg cattatccca ggcacctccg gcatttgact ggaacatgcc
      661 cattaatacg ccacttaccg ttacgcctgc caatacacca cccaatactt ccgggccaaa
      721 taagaaacca ataaccacag gagtaatcaa agcaatagca cccggcaaca tcatttcgcg
      781 gatagaagcc tgcgtagaga tttgtacgca tttttcgtat tcaggcttag ccttatactc
      841 cataatgccc ggaatttctc tgaactgacg acgaacttca tttaccatgt ccatagccgc
      901 tctgcccaca gcagaaattg ctaatgaaga aaatataaac ggaatcatac ctcctataaa
      961 caagcctgcc aaaacaggcg ctttgtaaat gtcgatagca tcaattttag ccactcctac
     1021 aaatgccgca aacaaagcta aagaagtcaa tgccgctgat gcaattgcaa atccttttcc
     1081 ggtagcagcc gtagtattac ctactgcatc caaaatatct gttctgcctc gtacttcttc
     1141 aggcaactgg ctcatctctg cgataccgcc agcattatct gcaataggac cgaatgcatc
     1201 gatagctaat tgcatggcag ttgttgccat catacctgca gctgcaatag ctacgccata
     1261 tagtccggcg cacatataag aaccgaagat accggcagct aaaacaataa taggcaacaa
     1321 ggtactttcc atccctacag ccaaaccacc aattacgttc gtagcatgcc cggttgatga
     1381 ttgacgtact attgacaata cagggcgttt gcccattgcc gtataatatt cagtaatgat
     1441 agacattaat gcaccaacta ctaatcccac gaaaatggca cccaacacac cgttagaggt
     1501 aaaggtatga ccacgcaaga ccatttctgt aggcaaaata aattttacca aacctataga
     1561 tgctatagcc gttaaaatta ttgaacccca gtttccttta ttcaatgccc cttgtacatt
     1621 atcattttcg ttagaaatgg taacaaagaa cgtacctatc caggaaaata agatacctgt
     1681 tccggcaata gccatcggta ataagattgg agctaatccg ccaaatttat cgttagaaat
     1741 tgtttcttga cccaatacca tggtagctaa aaccgttgcc acgtaagaac cgaataagtc
     1801 tgcacccata ccggcaacgt cacccacgtt atcacccacg ttatcagcga tagtagcagg
     1861 atttctaaca tcatcctcag gaattccggc ttccactttg cctacaaggt cggcacccac
     1921 gtcggcagcc ttggtataaa taccaccacc cacacgggca aacaatgcaa tagactcagc
     1981 ccctaatgag aatcctgtaa gtacttcaat agcacgtttt acttgctctg cccattccgg
     2041 agttccattg ttggtatgta caaataggaa ataaaacatg ataaacaaac tgcctaagcc
     2101 taacacggct aaaccagcta cgcccaagcc cattacagag cctcctgtaa aagacacttt
     2161 taaagcttga ggcaaactag tgcgagcagc ctgcgtggtg cgcacgttgg ctaatgttgc
     2221 cactttcatg ccaatatatc ctgctgttgc agaaaatatt gcaccggtta agaaagcaag
     2281 tgcaattaca gggctggagc cctcaccgcc tgcaaaacca agcacggcta acaatacagc
     2341 ggcaatgata ccaaagtaag ttaaaatttt ccattctgcc ttcaagaagg caatggctcc
     2401 atcggcaata tagccggcta attctttcat gttggcatcg cccgcatctt gtttttttac
     2461 ccagttgaat ttcacaaaag tgataagcaa gccaaataaa cccatggcgg gaactacaaa
     2521 aaatagtgga ttcataatac tataattatc tgatttttaa agcggtgcga atgtacgaca
     2581 aaaaagccat tattaacaat tcgagcctat ttattaacct aaaaacaaaa aaacctgcca
     2641 aagtgggcag gtttgttatt tgttttgctg aaatattgac tattcagcag ttttagtgca
     2701 tttagatttg ctttttttgc aatccttttt gtttttactg catttctcac ttttttc
//
LOCUS       DAOQII010000307         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_26321_length_2712_cov_4.235228, whole genome shotgun sequence.
ACCESSION   DAOQII010000307 DAOQII010000000
VERSION     DAOQII010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26321_length_2712_cov_4.235228"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PLF48_11295"
     CDS             complement(<1..1718)
                     /locus_tag="PLF48_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNF49960.1"
                     /translation="MSNKKYWSSLDELNETPAYKEQAANEFHINGDENDVTEFGSNRR
                     DFLKAMGFSVSIAALSSACQIPVKKAIPYTIDLRKAVPEIVPGVAEYFASTYCNGTDF
                     VNILVKTREGRPIKIEGNNESPLTLGGTNAHAQASVLGLYDVTRAQQPLKGGKKTDWK
                     TADAEIIQKLSSIAQAGGKIALVTGTVTSVLTKKAIEKFVAKYPTTNVVSYDAVSAYA
                     TRKANQISLGSPIIPTIHYDKADVIVGFNSDFIGTGISNVENAKKYIANRVPSKSNPK
                     MSRHYQFQSVATLTGSNADYRFQIKPSEEKQALINLLAKVGGSISGSAAVSVKETNEG
                     IDKAAKDLLASKGRSVVVSGTNDVQIQLLVNAINSVLGNYGTVVDTATTYNLKSGNDE
                     ALAALANDLKVGAYKAVIFLGANPVYNTGYAEIFKNAVKGAELSVSTADRQDETAQFC
                     TYLCPESHYLESWDIINPKTGFYGFVQPTINTIFSTRQVAETLMTWSGDTTPIATFAE
                     NEIAALSGGNYQNAVQAGFFGADGVGGGAAANAGSAIAAITPVTAANGMEAVLYQKIG
                     VGDGELA"
     gene            complement(1759..>2712)
                     /locus_tag="PLF48_11300"
     CDS             complement(1759..>2712)
                     /locus_tag="PLF48_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNF49961.1"
                     /translation="NKVFNENNKVAMTAFPQLTKEQVQDIFLYVKNSAVVEQPAAGAD
                     AGTATQAAPKSNTGIFYFLIVLLAILALVLWNIVSKLNKIVNAKEGVAEPEAKPFFGP
                     RFKKTALLLGSVLLVFNIFNGAIDLGRSQGYAPDQPIRFSHALHVGQNQIDCQYCHIG
                     VEKSRHASIPGTQICMNCHKAVNTGPKYGKDEIKKIYDYAGWDPDQAKFVREPKNIEW
                     VKIHNLPDHVYFNHSQHYKVGGIKCQTCHGKVEEYEVMKQFAPLSMGWCINCHRQTEV
                     KFNDNKYYSIFQRYQKELKEGKKDKITVADVGGTDCQKCHY"
BASE COUNT          774 a          560 c          477 g          901 t
ORIGIN      
        1 gccaattcgc catctcctac acctattttt tgatagagta cagcttccat gccattagca
       61 gcagttaccg gagtaatggc cgctattgca ctacctgcgt tagcagcagc accgccacca
      121 acaccatcag caccaaaaaa tcctgcttga actgcatttt gataatttcc tcccgataaa
      181 gcggcaattt cattttcagc aaaagtagca attggtgtcg tatctccaga ccaagtcatc
      241 agtgtttctg ctacctgacg ggtactgaaa atagtattaa ttgttggctg aacaaaaccg
      301 taaaatcctg tttttggatt tataatatcc caagactcta agtaatgtga ttccggacat
      361 aaataggtac agaattgagc cgtttcatct tgtctgtcag ccgtagaaac agaaagctca
      421 gcacctttta ctgcattttt aaaaatttct gcatagccgg tattataaac aggattagct
      481 cccaagaata taacggcttt atatgctcct actttcaaat catttgctaa agcagctaaa
      541 gcttcatcat ttccagattt taagttgtaa gtagttgcgg tatcaacaac tgttccgtag
      601 ttacctaaga cactgttaat agcatttacc aataattgaa tttgtacatc gttagtacct
      661 gaaaccacta cagaacgtcc ttttgatgcc aacaaatctt tagcagcttt gtcaatacct
      721 tcgttagttt ctttgacaga tactgcggca ctaccactaa tacttccacc cacttttgcc
      781 aacaagttta ttaatgcctg tttttcttca gatggtttaa tttgaaatct gtaatccgca
      841 ttagaacctg ttaatgtagc cacagactga aactgataat ggcgtgacat tttaggatta
      901 gatttgctgg gaacacggtt tgctatgtat tttttagcat tttccacatt agaaataccc
      961 gttccgataa aatcactgtt aaagccaaca attacatctg ctttgtcata atgaatggta
     1021 ggaataattg gcgagcctaa agaaatctga tttgctttac gtgttgcata agcagataca
     1081 gcgtcataac tcactacatt cgtagtagga tattttgcca caaatttttc aattgctttt
     1141 ttggtcagta ctgatgtaac cgttccagta accaatgcaa ttttgccacc ggcctgtgca
     1201 attgaactta atttttgtat aatctctgca tctgctgttt tccaatcggt ttttttccca
     1261 cctttcaaag gttgttgggc tctggtaaca tcatacaatc ccaaaactga agcttgagca
     1321 tgcgcattgg ttcctcctaa agtcaaaggc gattcattat tgccttcaat ttttatagga
     1381 cgaccttcac gtgttttcac taaaatattg acaaaatcag taccattaca ataggtggat
     1441 gcgaaatatt ccgcaacacc gggtacgatt tcaggaactg cttttcttaa atctatcgtg
     1501 taaggaatag cttttttaac cggaatctga caggcagatg atagtgcagc aattgaaacg
     1561 ctgaaaccca ttgcttttaa gaaatctctg cggttagagc cgaactctgt tacatcattt
     1621 tcgtcgccgt tgatatgaaa ctcattagcg gcttgttcct tatatgccgg agtttcattc
     1681 aactcatcta aactcgacca gtattttttg ttactcatta ttaggaatgt tataacgatt
     1741 tatacaaata atttttaatt aatagtgaca tttttgacaa tcagtacctc caacatcagc
     1801 tacggtaatc ttatcttttt taccttcttt caactctttt tggtagcgtt ggaaaataga
     1861 gtagtattta ttgtcgttaa atttcacttc tgtctgacgg tgacaattga tacaccatcc
     1921 catagaaagc ggtgcaaatt gtttcatcac ttcgtattct tctactttgc catgacatgt
     1981 ttggcattta atgccaccaa ctttatagtg ttgtgagtgg ttgaaataaa cgtgatccgg
     2041 caagttatga atttttaccc attctatatt tttaggttca cgaacaaatt ttgcctgatc
     2101 tggatcccaa cctgcgtaat cataaatttt cttgatttca tcttttccgt atttcggacc
     2161 ggtattgact gctttatgac agttcataca aatttgtgta cccggaatac ttgcatggcg
     2221 acttttttcc actccgatat ggcaatattg acaatcaatt tggttttgtc ctacgtgtaa
     2281 tgcatgggag aaacgaatag gctgatcagg cgcataaccc tgagagcgac ctaaatcaat
     2341 agctccatta aagatgttga acactaataa tactgaaccc aacaacaagg ctgtcttttt
     2401 aaagcgaggg ccgaagaatg gttttgcttc cggttctgct acaccttctt tagcattcac
     2461 tattttattc agtttggaaa cgatattcca aagcactaat gctaatattg ccaacaagac
     2521 aatcaggaaa tagaaaatac ctgtatttga tttgggtgca gcttgagtag cagtgccagc
     2581 atctgcgccg gcagcaggtt gttctacaac tgcgctgttt tttacatata aaaagatgtc
     2641 ttgtacttgt tctttagtca gctgaggaaa ggcagtcatc gcaactttat tgttttcatt
     2701 gaaaactttg tt
//
LOCUS       DAOQII010000308         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_26849_length_2683_cov_4.404490, whole genome shotgun sequence.
ACCESSION   DAOQII010000308 DAOQII010000000
VERSION     DAOQII010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26849_length_2683_cov_4.404490"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLF48_11305"
     CDS             <1..1223
                     /locus_tag="PLF48_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNF49962.1"
                     /translation="YIYYSAYLLSGTLYLFVKNSFAFEYLWPNYPHLDFLMRKVMLSM
                     YLLASVTFLRGFIKSRVKAEGLQRISKQFIIAGIFITFISFLFGLLTPQLQQLFKISQ
                     SIFAIACYIMILFTFITAYLKSNNRSLIFFTLVYFLSFSFFLFYPQPEFARDFFGVSL
                     GQIYTYSNAFIIAIIISFTTVYRVLFILRQNEKMKTQMSALYVSNNYALVEGQYRERQ
                     RVGQELHDGIGVLLSSVKMKMSALKTTTPEDKIALQKLLQTTDDICSTTRKLSHVFLP
                     PTLNKFGIKTALQDMFDAFIIGSKTALPYSILISDKLSFVSQNTIYDLLQQLLSYFKQ
                     NRPDTLQIRLVELKTEQRVQLRIVYSGIKLNPAQPAIQNLLSTIEVLNGKYENTLLNA
                     WTNSFTIEIPVTFE"
     gene            complement(1210..2178)
                     /locus_tag="PLF48_11310"
     CDS             complement(1210..2178)
                     /locus_tag="PLF48_11310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNF49963.1"
                     /translation="MKFRTEIFLQKSSLKISHQDKILLAGSCFAENMQAYFRRFLFDV
                     LSNPHGVIYNPVSLHRALQDVANKKIYELKDLHFYNELYFSAAHHTSFSDTDSSVCLE
                     KINTSITQAHQHLQNANVLIITLGSAYVYAWNETGANYFGTPSEIVANCHKLPNTFFQ
                     KRLLSLEEIVQTLQDTIRIFPDKKIIFTVSPVRHWRDGAVENMRSKSLLIEAIHRVAE
                     QSAAVYYFPAFELMMDDLRDYRFYEEDLLHPNKTAVNYIWDKFAATFFDEETQLLNKK
                     IDAIQAMKSHRPRFEDTQAYQAHKQKIEQEINVLTTQYPYLDFTQK"
     gene            2236..>2683
                     /locus_tag="PLF48_11315"
     CDS             2236..>2683
                     /locus_tag="PLF48_11315"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNF49964.1"
                     /translation="MYRSKIHFFLAFLLWNYAYSFAITYDNQVFVSYIKTVQFFPTEN
                     EMGFAAIQLNSPETLVLHFDDLSNKSKTFYYTVQQCRADWSPSDMNIMEYTEGFTEST
                     INDYSYSNGTKVPYTHYTMTFPNNDMRVTKSGNYALIVYETNKENPV"
BASE COUNT          805 a          525 c          458 g          895 t
ORIGIN      
        1 attacattta ttacagtgcc tatcttctat ccggaacact ctatcttttt gtcaagaaca
       61 gttttgcctt tgagtatctg tggccaaatt atccgcatct ggattttctg atgcgcaaag
      121 tgatgctatc catgtatttg ctggcatctg ttacattttt gagagggttt atcaaatcca
      181 gagtgaaggc tgaagggcta cagcgcattt ctaaacaatt tattattgca ggtatcttta
      241 ttacttttat ctccttttta ttcggattat taacgccaca gttgcaacag cttttcaaaa
      301 tttcgcaaag catttttgcc attgcctgtt atatcatgat cttatttact tttatcacag
      361 catacctgaa atcaaataat cgttcactta ttttttttac gttagtttac tttttatctt
      421 tttccttttt ccttttttat ccgcaaccgg aatttgcccg cgattttttt ggtgtcagtc
      481 tcggacaaat ttatacctac agcaatgcat ttattattgc catcatcatc agctttacta
      541 cggtttaccg ggtgttgttt atccttagac aaaatgaaaa aatgaaaaca caaatgagcg
      601 cactctatgt ttccaataat tatgcattgg ttgaagggca ataccgggaa cggcaacgtg
      661 taggacaaga actgcatgac ggtataggtg tattgctatc ttctgtaaaa atgaaaatga
      721 gtgcgctgaa gacaactaca cctgaagaca agattgcctt acaaaaactg ttacaaacaa
      781 ctgatgatat ttgctctaca acaagaaaac tatcgcatgt ttttttaccg ccaacgctaa
      841 ataaattcgg aataaaaaca gccttacagg atatgtttga tgccttcata ataggctcaa
      901 aaaccgcctt accctactcc atactgatct cagacaagct cagctttgta tcacaaaaca
      961 ccatttatga tcttcttcaa caattgcttt cctactttaa acaaaaccgt ccagatacct
     1021 tacaaatcag gcttgtagaa ctaaaaacgg aacaacgtgt acagcttcgt attgtatatt
     1081 ccggcataaa attgaatccg gctcaacctg ctattcaaaa cttgctttct actattgaag
     1141 tattgaacgg caaatatgaa aacaccttgc tcaatgcctg gacgaacagc ttcacgatag
     1201 aaattccggt tacttttgag taaaatctaa gtatggatat tgggtagtca gcacattaat
     1261 ttcttgttct attttttgtt tgtgtgcctg ataggcttgg gtatcttcaa atctagggcg
     1321 gtgcgatttc atggcctgta tggcatcaat ttttttattg agcaactgtg tttcttcatc
     1381 aaaaaatgta gcagcaaatt tatcccaaat atagtttacc gctgttttat tgggatgtaa
     1441 taagtcttct tcataaaaac gataatcgcg caaatcgtcc atcatcaatt caaaagccgg
     1501 aaaataatat accgcagctg attgttctgc aacacggtga atggcttcta tcaacaaaga
     1561 tttactgcgc atattttcta ctgctccatc acgccagtgc cgcactggac ttaccgtaaa
     1621 gatgattttt ttatcgggaa aaatgcgaat ggtgtcttgc aatgtttgta ctatttcctc
     1681 taaagaaagc aggcgtttct gaaaaaacgt atttggcaat ttgtggcaat tggcaactat
     1741 ttctgaagga gtaccaaagt aatttgcacc tgtttcattc caggcatata catacgcact
     1801 tcccagtgta ataattagca cattcgcatt ttgcaaatgc tgatgagctt gtgtaattga
     1861 agtatttatt ttttccagac aaacagaact gtctgtatca ctgaatgagg tgtgatgagc
     1921 tgctgaaaaa tataattcat tataaaaatg cagatccttt aattcatata tctttttgtt
     1981 tgcgacatcc tgcaaagctc tatggagtga aacaggatta taaataacac catgcggatt
     2041 ggacaagaca tcaaacaaaa atcgccggaa ataagcctgc atattttctg caaaacaaga
     2101 acctgcgagc aagattttat cctgatggct gattttcagc gagctttttt gtagaaatat
     2161 ctccgttcgg aacttcataa agaattattt taatcaaaat cttatttttg gaaagaaatt
     2221 tgctgtactg ctttcatgta tcgttcaaaa atacattttt ttttagcttt ccttttatgg
     2281 aattatgcgt attcttttgc cataacctat gacaatcagg tttttgtttc atatatcaag
     2341 acggtgcagt tttttcctac cgagaatgag atgggcttcg ctgccataca attgaattca
     2401 cctgaaacac tggttttaca ttttgatgat ttatccaaca aatccaaaac tttctattat
     2461 accgtgcagc aatgccgtgc cgactggtcg ccttcggata tgaatatcat ggagtatacc
     2521 gagggcttta ccgaatctac aatcaacgat tattcttatt ccaatggtac caaagtaccc
     2581 tatactcatt ataccatgac ttttccgaat aatgacatgc gtgttaccaa gtcggggaat
     2641 tatgccttaa tcgtgtatga aacgaataaa gaaaatccgg ttt
//
LOCUS       DAOQII010000309         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_27062_length_2670_cov_3.764436, whole genome shotgun sequence.
ACCESSION   DAOQII010000309 DAOQII010000000
VERSION     DAOQII010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27062_length_2670_cov_3.764436"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..706)
                     /locus_tag="PLF48_11320"
     CDS             complement(74..706)
                     /locus_tag="PLF48_11320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF49965.1"
                     /translation="MSKTIHTAQQYWDVRSDLFANYYKKPSMFDKMFRKAVYTRTAVM
                     LNTIKECGKCTVLDIGSGPGVNSVTWLKNSEASFLLGIDFAENMNEYARKNAEAEGVG
                     DRCKFVAGDFLTYDFKGEKFDVACAAGVTDYVKDIEALVKKMDIVTNKAFVASWPTGG
                     LRMALRRYRYTCPVFQYNEADVRRLHEGCNIKSLDIIPMQGGLMSVARKY"
     gene            complement(657..1577)
                     /locus_tag="PLF48_11325"
     CDS             complement(657..1577)
                     /locus_tag="PLF48_11325"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNF49966.1"
                     /translation="MENNRPPQFTLSIDWEDFSQLLGRDGSGVLTPPLPVTIDRQTDI
                     MLEMLSDAGHKATFFVLGMTAQHRPDLVKKIAGEGHEIGLHGQWHINMRTLTKEQAWS
                     DLSDSYKLVTDIIGAPVYGFRAPYFSVDESNYYVLEMLADLGLIYDSSIFPLKLKRYG
                     VANFNKEDAFYVLPNGKQIVELPLTVARMYGKNIPVAGGGYMRAFPKFFLKKVFKKLN
                     RDEANVMLYMHPYEFDTKSIDCSANYPENFSYSKIKTAIINLRWNLFRQSIRGKLKYL
                     LKQYQFTTCLNKANYVKNNTHSPAILGRTQ"
     gene            complement(1567..>2670)
                     /locus_tag="PLF48_11330"
     CDS             complement(1567..>2670)
                     /locus_tag="PLF48_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF49967.1"
                     /translation="IENHELGIYSIRHDLVKAIASVYDTTVLTEMDGSFQNGDLHTIV
                     NFVDVGKAVLNPAKALKYNARLRKALKEIKPDVCLTFTIRPAIYGNIVTRSLQIPTIS
                     TITGTGPLFDSKSLSYTIARQLYKKVLKKTKFVFFPNYDDLNGFIERKYITRAQGRRV
                     PGSGINYEQFVPMPSQRNEDGKFVFLFISRLLKDKGTMEYVEAASLLMEKYPHAEFHI
                     VGPLWTGNKKSLTVTPEELKSWIEKKWIVYHDKQNDVKPFIANADCVVMPSYREGMSN
                     VLLEAASMARPLIATNVTGCRDIVDDGVNGFLCEVKNGKDLADKMVMMMNLSAEQRME
                     MGKKGREKMIKEFDKKIVIQKYLEAIKEVLDGK"
BASE COUNT          775 a          592 c          433 g          870 t
ORIGIN      
        1 aattatggct atttaagtat aaaaaaagcc cccgaaaatt cggaggcttt gaaaacgttt
       61 aagaatattg aaattaatat tttctggcca cactcatcaa accgccttgc attggaatga
      121 tatcaaggct cttgatatta cagccctcat gcaatctgcg aacatctgct tcattgtatt
      181 gaaataccgg acaagtataa cgataacgac gcaaagccat tcttaaacca cctgttggcc
      241 atgaagcaac aaatgcttta tttgtaacaa tgtccatctt ttttacaagt gcttctatgt
      301 cttttacata gtcagtaaca cctgctgcac aagcaacatc aaatttttcg cctttaaaat
      361 cataggtcaa aaaatcacct gctacaaatt tgcaacgatc gcctactcct tccgcttcgg
      421 cattctttct tgcatattca ttcatattct cagcaaaatc aattcccaaa agaaaacttg
      481 cttctgagtt tttcaaccaa gtaacactat tcactccggg accggaacca atatccagca
      541 cggtacattt tccacattcc ttaatggtgt tcagcatcac tgcggtacgg gtgtaaacag
      601 ccttccgaaa catcttatca aacatggaag gctttttgta ataattggcg aatagatcac
      661 tgcgtacgtc ccagtattgc tgggctgtgt gtattgtttt tgacatagtt tgctttgttt
      721 aaacaggttg taaattggta ttgtttcagt aaatatttta gttttcctcg aatggattga
      781 cgaaataaat tccagcgcaa gttaatgatt gctgttttaa tttttgaata tgaaaaattt
      841 tcagggtagt tggccgagca atctatgctt ttggtgtcaa attcataagg atgcatatac
      901 aacatcacat tggcctcatc ccggttgagt tttttaaata ctttcttcaa aaagaatttt
      961 ggaaaagccc tcatataacc accaccagct accggaatat tttttccata cattcttgcg
     1021 accgtaagtg gtaattcaac tatttgttta ccatttggta aaacataaaa tgcatcttcc
     1081 ttattgaagt tggcaacacc atatcgtttt aatttcaacg gaaaaatact gctgtcataa
     1141 atcaaaccca gatcagccag catttccaga acataatagt tggattcatc aactgaaaaa
     1201 taaggtgccc tgaaaccgta aaccggtgcc ccgataatat cagttactaa tttatagcta
     1261 tcactgaggt cgctccaagc ttgctcttta gtcagcgtac gcatattgat atgccattga
     1321 ccatgcaatc caatctcatg cccttcgccc gctatttttt tcaccagatc gggtctatgc
     1381 tgcgccgtca ttcccagaac aaaaaaagtt gctttatgcc ctgcatcact caacatttcg
     1441 agcataatgt ctgtttgcct gtcaattgtt accggtaatg gcggtgtgag aacaccactg
     1501 ccatcacgac ccagtaactg actgaaatct tcccaatcaa ttgacaatgt aaattgagga
     1561 ggtctattat tttccatcta atacttcctt tatagcttcc agatatttct gaatcacaat
     1621 ttttttatca aactctttta tcattttttc tcttcccttt ttccccattt ccattctctg
     1681 ttcagcagat aaattcatca tcataaccat tttatcagcc agatctttcc cgttttttac
     1741 ttcgcacaaa aatccattta caccatcatc cacaatgtcg cgacagccgg tgacattagt
     1801 cgctatcaat ggccgggcca tactggctgc ttccaataaa acattgctca tcccttcgcg
     1861 gtaggaaggc atcaccacac aatcagcatt ggcaataaaa ggctttacat cattttgttt
     1921 atcgtgatag acaatccatt ttttctctat ccagcttttt agttcttcag gcgtaaccgt
     1981 caaagatttt ttattgccag tccatagcgg gcctacaata tgaaactcgg catgtggata
     2041 tttttccata agtaaagaag cagcttctac atattccatc gttcctttat ctttcaacaa
     2101 cctgctaatg aaaagaaaaa caaactttcc gtcttcattt ctttgtgatg gcatgggtac
     2161 aaactgctcg tagttgatac ccgaaccggg cactcttctg ccctgtgccc ttgtaatata
     2221 ttttctttca ataaatccat tgaggtcatc ataatttgga aagaaaacaa atttggtttt
     2281 cttcaacacc tttttgtaaa gctggcgggc aattgtatat gaaaggcttt tactgtcaaa
     2341 caagggtcct gttccggtaa tggtagatat tgtaggaatc tgtaaagaac gagttacgat
     2401 atttccataa atagcagggc gaatagtgaa cgtaaggcac acatcgggtt ttatttcttt
     2461 taaagctttt ctcaatcttg cattgtactt taatgctttt gcaggattta aaactgcttt
     2521 gcctacatcc acaaaattta caattgtatg caaatcgcca ttttgaaaac ttccgtccat
     2581 ttctgtcagc acggttgtat catacactga tgctatggcc ttcaccaaat cgtgccggat
     2641 agaataaatg cccaattcgt gattttctat
//
LOCUS       DAOQII010000310         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_28280_length_2605_cov_4.584706, whole genome shotgun sequence.
ACCESSION   DAOQII010000310 DAOQII010000000
VERSION     DAOQII010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28280_length_2605_cov_4.584706"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLF48_11335"
     CDS             complement(<1..850)
                     /locus_tag="PLF48_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNF49968.1"
                     /translation="MTEFNPNGVGIKNGHIFSLPYSYQEAKLVLLPVPWEVTVSYNAG
                     TANAPEAILEASSQLDLFDAHAENEFLNGIYMLKISDEWKQKNKKFRIKANEYIKLLE
                     SGADLSANTAVQKNVQEINEECDALRKWVFEETTKLLNENKLVGLIGGDHSTPLGYFQ
                     AIGKKYGEFAIVQFDAHADLRNAYEGFTYSHASVMFNALKIEELTNLFQIGIRDLSPE
                     EYALTQSHPKIKTFFDWDLKEQLIHGKSWADITNDIIEQLPQNIYISFDIDGLERSFC
                     PNTGTPV"
     gene            946..1347
                     /locus_tag="PLF48_11340"
     CDS             946..1347
                     /locus_tag="PLF48_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNF49969.1"
                     /translation="MKKIHIVILVFIAILVGVLISMLGNFSRYETFESAYAKKGKEIN
                     VVGVLNKAKGVDYNPEANPNLFSMYVTDSKGLEKKVVVHKAKPRDIEKSEKVVVIGKM
                     NGDTFEANDILMKCPSKYKNQEKDANGGRSI"
     gene            1446..>2605
                     /gene="ccsA"
                     /locus_tag="PLF48_11345"
     CDS             1446..>2605
                     /gene="ccsA"
                     /locus_tag="PLF48_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNF49970.1"
                     /translation="MEKLGHLFAISAFVFAFAATLSFFLATIKSTPDIEKANWLKAGR
                     YFFLAHAISVFGIFSFLYYIIHSHQFQYSYVWQHTSKDLPWYYQFSALWSGQEGSTTL
                     WMFWHAVLGIVLLKKAKEWESGVMTFVSLAQLLLGSMLLGIYILHYKTGSSPFALMRE
                     TMNIPIFKMQPDWVAQDGTGLNPSLQNYWMVIHPPTLFLGFAACTIPAAFALTSLWLR
                     KYTEWVKPVFPWALFALMILGAGVLMGGAWAYEALSFGGFWAWDPVENASLVPWLILA
                     GAVHTLLIYKHTGHSLFTTYVLFIVCFLLVLYSSFLTKSGILGDASVHSFTDLGMSGL
                     LISIMVAFIVPSLYLLISRKREVPAVEAEEDISSREFWMFIGSLVLLFSALI"
BASE COUNT          753 a          435 c          520 g          897 t
ORIGIN      
        1 caacgggcgt acctgtattt ggacaaaagc tacgctctaa tccatcaata tcaaaactga
       61 tataaatatt ttgtggaagc tgttctatga tgtcattcgt gatgtcagcc caagatttac
      121 catgtattag ctgttctttt aaatcccagt cgaaaaaagt tttaatcttg ggatgcgatt
      181 gcgttaaagc atattcttcc ggtgataaat ccctgatacc aatttgaaat aaattcgtca
      241 gctcttctat tttcagtgca ttaaacatga cagaagcatg cgaataagta aaaccttcat
      301 acgcatttcg caagtcagca tgtgcatcaa attgtacgat ggcaaattcg ccatattttt
      361 ttcctattgc ctgaaaatag ccgagtggag tactatggtc gccaccaatt aatccaacca
      421 gtttattttc atttaataat ttagttgttt cttcaaatac ccatttgcgc aaagcatcac
      481 attcctcatt tatttcttgt acattttttt gcactgcagt attagcactt aagtcagcac
      541 ctgattcaag caatttaata tattcattgg ctttgatgcg aaatttctta tttttttgtt
      601 tccattcgtc tgaaattttg agcatgtaaa tgccatttaa aaactcattt tcagcatgtg
      661 catcaaataa atccagctgt gaactggctt ctaaaattgc ttcaggagca tttgccgtgc
      721 cggcattgta agaaacggtt acttcccatg gaacaggcaa taaaaccaat tttgcttcct
      781 gataggaata tggaagtgag aagatatgtc cgttttttat accaacaccg tttggattaa
      841 attctgtcat tttttgcttt tggtcaaagg tagtaatgtt aattcattag tcttaatctt
      901 ttgtttacaa agtgaagcgt atttttacaa tacaatttca acaacatgaa aaagatacac
      961 attgttattc ttgtttttat tgccattctg gttggagtac taattagtat gttgggcaat
     1021 tttagccgct acgaaacatt tgaaagtgct tatgccaaaa aaggtaaaga aataaatgta
     1081 gttggtgtac tgaataaagc aaaaggggtg gactataatc cggaggcaaa tcccaattta
     1141 ttttccatgt acgttacaga ttcaaaagga ttggaaaaga aagtagttgt tcataaggcg
     1201 aaaccccgtg atattgaaaa atcagaaaaa gtagtagtaa tcggtaaaat gaatggagat
     1261 acctttgaag ctaacgacat tttgatgaaa tgcccgtcta aatataaaaa tcaggaaaaa
     1321 gatgcaaacg gagggagaag tatttagttg aggtaaggtg taagtaataa ataataagta
     1381 ataagcaggc aggagtttaa tttaaattta taattcaaaa tctaaaattc aatattaatt
     1441 attctttgga aaagttaggt catttatttg caatcagtgc gtttgttttt gcattcgcag
     1501 caacgctatc atttttttta gcaaccatta aatcaactcc tgacattgaa aaagcgaatt
     1561 ggctgaaagc aggacgatac ttttttttag ctcatgcaat aagtgtattt ggcatattca
     1621 gctttttata ttatatcatc catagccatc agtttcagta tagctatgta tggcagcata
     1681 cgtcaaaaga tttaccctgg tattatcagt tttccgcatt atggagcgga caagagggca
     1741 gtactacgtt atggatgttt tggcatgctg ttttaggtat tgtcttattg aaaaaagcaa
     1801 aagaatggga gagtggggtg atgacatttg tttcacttgc ccagctgctc ttgggttcta
     1861 tgttgttagg tatttatatc ttgcattata aaacaggttc atcgcctttt gcactgatgc
     1921 gggagacgat gaatattccc atttttaaaa tgcaacccga ctgggtagct caggatggaa
     1981 cgggattaaa tccgtcttta caaaattact ggatggtcat ccatccgcca actttgtttt
     2041 taggattcgc tgcgtgcaca atcccggctg catttgccct tacatcactc tggctacgca
     2101 aatatacaga gtgggtaaag cctgtttttc catgggcatt atttgctttg atgatattgg
     2161 gtgcaggtgt gttgatgggt ggtgcatggg catatgaagc tttgagcttc ggcggttttt
     2221 gggcttggga tccggttgaa aatgcttcgc ttgttccatg gctgatttta gccggagccg
     2281 ttcatacact attgatttat aaacataccg gacattcatt atttactacg tatgtcttgt
     2341 ttattgtttg ctttttgttg gttttgtatt cttcattttt aactaagagt ggcatcttgg
     2401 gtgatgcttc cgtccattca tttacagatt tgggtatgag cggattgctc atttctataa
     2461 tggttgcttt catcgtgcca tccttgtatt tgttgatttc cagaaaacgt gaagtgccgg
     2521 ctgtggaagc agaagaagat atttcatcca gagaattttg gatgtttatc ggttcgctgg
     2581 tcttattatt ttcggcgtta atcat
//
LOCUS       DAOQII010000311         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_28465_length_2595_cov_7.085039, whole genome shotgun sequence.
ACCESSION   DAOQII010000311 DAOQII010000000
VERSION     DAOQII010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28465_length_2595_cov_7.085039"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLF48_11350"
     CDS             <1..522
                     /locus_tag="PLF48_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49971.1"
                     /translation="EIYQELNEPEKIGDLYMLMKKKDQALPYYQKVITGYTAKDQYIK
                     ASLIYRKKLQDNTNAQQLLLQGWLKDKDAVNCINNYFSEIKEPDALIQEIQNIYNNHT
                     PHHQKGKLLEALKHEYNKHPDMEQPTREIAYAIIAELMVKNRAILKELTAFTKDDKQV
                     LTDIMQYNLYNAV"
     gene            617..1447
                     /gene="prs"
                     /locus_tag="PLF48_11355"
     CDS             617..1447
                     /gene="prs"
                     /locus_tag="PLF48_11355"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNF49972.1"
                     /translation="MLTLNLDNSFKPFENKEINFESFVFSGGEPHIKILDKVQKEDVV
                     YVTQRVNNFNDLGLVCMATDALRRMGVNDIRAFIPYFPGARQDRLMIPGEPLSVKVYA
                     TLINQLKLQELTIFDPHSDVAPALLNNCKVVPNYAFIEEVIRSINTSVVLVAPDGGAL
                     KKIYKLSAALGGLEVVECSKQRNVSTGKITGYKVYADDLSGKTCLVVDDICDGGATFI
                     RLAQELQNKNAKQLYLAVSHGIFSKGYAELIQYYNTIFTTNSIKDITETGIKQIKINL
                     "
     gene            1444..2388
                     /locus_tag="PLF48_11360"
     CDS             1444..2388
                     /locus_tag="PLF48_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNF49973.1"
                     /translation="MNVDDIKAAFLGLAIGDALGVPVEFKSRTALLSNPVKTMLEYGC
                     WNQPKGTWSDDSSLTFCLADSLCGGYNLNDIAQKMLLWYQHAYMTAHGEVFDVGGTTR
                     HALMRLIDGCSPTISGNLFESDNGNGSLMRILPLAFYLQHEEDLAIIYSKVKEVSAIT
                     HAHFRSVYACFVYVVYLIELIQEKDKHKAYSSMQKKIIDFTEQNQFNKQETNLFSKII
                     KEDIAKYSTEEIKSDGYVLHTLEASLWCMMNTDNYEDAVLSAVNLGGDTDTTACVTGG
                     MAGLYYGMKSIPKDWISTVVKSEQIEQLSLRFYEALIN"
     gene            2390..>2595
                     /locus_tag="PLF48_11365"
     CDS             2390..>2595
                     /locus_tag="PLF48_11365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HNF49974.1"
                     /translation="MNTNQQHKNISKFLSLVLRHMPDTISLNLDENGWADISELIEKC
                     NNHNVLLNIQLLEEIVTTNDKQRFA"
BASE COUNT          945 a          414 c          506 g          730 t
ORIGIN      
        1 gaaatttatc aggaattgaa tgaacccgaa aaaatcggcg atttgtacat gctcatgaag
       61 aaaaaagatc aagccttacc gtattatcaa aaagtaatta caggttatac ggctaaagat
      121 caatatatta aggcctctct gatttacaga aaaaaattgc aagataatac caatgcacag
      181 cagttattac ttcagggatg gctaaaggac aaagatgccg tgaattgtat caataattat
      241 ttttcggaaa taaaagaacc tgatgcactt atacaagaaa tacaaaacat ctataacaat
      301 cacacgccgc atcatcaaaa aggcaaatta ctggaagcat taaaacatga atacaacaaa
      361 catccggaca tggaacagcc aaccagagaa attgcttacg ccattattgc agaactaatg
      421 gtaaaaaaca gagcgatact caaagaactc actgccttta caaaagacga caaacaagta
      481 ctcaccgata ttatgcaata caatttgtat aatgccgtct gaaaaaataa aaaaaacact
      541 ttgcgtaaaa ataacacata aaaaagttgc aagaatcatt tttatcaatt aactttgcgt
      601 aataaataca ctaattatgt tgacgttaaa tttagataat tcgtttaagc cttttgaaaa
      661 taaagaaatc aactttgaaa gctttgtgtt ttccggtggc gaacctcata taaaaatact
      721 ggacaaagta caaaaggaag atgtggttta tgtaacacaa cgggttaata attttaatga
      781 tttagggctc gtttgtatgg caacagatgc gcttcgcaga atgggtgtta atgatatcag
      841 ggcttttatt ccctattttc ccggtgcaag acaagaccgg ctaatgatcc ccggagaacc
      901 tttaagtgta aaagtgtatg caacacttat aaaccagcta aagctgcaag agcttaccat
      961 ctttgatccg cattcagacg tagcacctgc attgttgaat aactgcaaag tagtaccaaa
     1021 ctatgcgttt attgaagagg tcatcaggag tataaacaca tccgttgtac tggttgcgcc
     1081 ggatggaggt gcgctcaaga aaatctataa actgtctgct gcgcttggtg gcctggaagt
     1141 ggtggaatgc agcaaacagc gaaatgtaag cactggaaaa ataacaggct acaaagtata
     1201 tgctgatgat ttgagtggca aaacgtgttt ggttgtagat gatatctgcg atgggggtgc
     1261 aacatttata cgactggctc aagaactaca aaataaaaat gcaaagcagt tatatctggc
     1321 agtgagtcac ggaatattca gcaaaggata tgcggagttg atacaatact acaacacgat
     1381 atttacaacc aactcaatta aagacataac agaaacaggc ataaaacaga ttaaaattaa
     1441 tttatgaatg tagatgatat taaggctgct ttcttaggtt tagctattgg cgatgcattg
     1501 ggagtgccgg tagagtttaa aagcagaaca gcgttacttt ctaatcctgt aaaaacaatg
     1561 ctcgaatatg gttgttggaa tcagccaaaa ggaacatgga gtgatgacag ttcattgact
     1621 ttttgtttag ctgatagtct gtgtggagga tataatttga atgatattgc tcaaaaaatg
     1681 ttgctctggt atcagcatgc atatatgaca gcacatgggg aagtatttga tgttggcggt
     1741 acaacgcgtc atgcattaat gagattaatt gatggctgtt caccaacaat atcgggcaat
     1801 ttgtttgagt cagataatgg taatgggtca ttaatgagga tacttccact tgctttctat
     1861 ttgcagcatg aagaagattt agcaataata tatagcaaag taaaagaggt atcagctatc
     1921 acacatgcac attttcgctc tgtatatgct tgttttgttt atgtagttta tttgatagaa
     1981 ctaattcagg aaaaagataa gcacaaagcc tattcaagta tgcaaaagaa aattattgat
     2041 tttacagaac agaaccagtt taataaacag gaaacaaacc tgttctctaa aattataaaa
     2101 gaagatattg ctaaatattc aacagaagaa attaagtctg atggctatgt tttgcataca
     2161 ttagaagcat ctttatggtg tatgatgaat actgataatt atgaggatgc agttttaagt
     2221 gcagttaatc ttggaggtga tactgatact acagcatgtg ttaccggagg catggcaggt
     2281 ctttattatg gaatgaaatc tatacccaaa gattggataa gtacagtcgt aaaaagtgaa
     2341 cagatcgaac agttgtcatt acgattttat gaagcactaa ttaattaaaa tgaacaccaa
     2401 tcaacaacat aaaaatataa gcaaatttct tagccttgtt ttacggcata tgccggatac
     2461 tatttctttg aatctggatg aaaacggctg ggcagatatt tctgaactca ttgaaaaatg
     2521 caataatcat aatgttttac taaatatcca actgcttgaa gaaatcgtaa ccacaaacga
     2581 taaacaacgg tttgc
//
LOCUS       DAOQII010000312         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_28784_length_2579_cov_4.474247, whole genome shotgun sequence.
ACCESSION   DAOQII010000312 DAOQII010000000
VERSION     DAOQII010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28784_length_2579_cov_4.474247"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /gene="pyk"
                     /locus_tag="PLF48_11370"
     CDS             complement(<1..1400)
                     /gene="pyk"
                     /locus_tag="PLF48_11370"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNF49975.1"
                     /translation="MNTTRRKTKIVATIGPASNTYERLVELYESGVNVFRLNFSHGRH
                     EDHLEVIEQITKLNENLHAHVAILADLQGPKMRTGLMQNNAVLLREGHTIRVTNEEVI
                     GTSELISVTYDFLAQDLKPGEWILIDDGKLELKVAKIIDNKTIECKIISGGILSSKKG
                     FNLPNTEISLPSLTEKDKRDLEFIVQQPVNWIALSFVRKAEDIQELREHIHRMRAHAK
                     LIAKIEKPEAIENIDSIISVSDAVMVARGDLGVELPIEEMPLIQKSVIHKCILKAKPV
                     IIATQVMESMMEMPRPTRAEITDVANGVLDGADAIMLSGETSVGKNPAKVVQTIDKII
                     RRTEQETSIYNKHHLPNKNSHTFISDAICYNACKIAEDVNAKAINGMTYSGYTAFMLA
                     SNRPKSDIHIFTSNPSLLNTLSLVWGVDAYYYNKFESTDDTIYDLIDKLKEDEQVHHG
                     DFVISVTSMPIRGKGRA"
     gene            complement(1345..1824)
                     /locus_tag="PLF48_11375"
     CDS             complement(1345..1824)
                     /locus_tag="PLF48_11375"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNF49976.1"
                     /translation="MAKRQIIDLYFDFSVWGINSAMEDYRICWHLNELLHWKMKRIAD
                     IEFNNTKQKSFSFFHAYSYSNVIDFYTLEFIQNKNAGNYLIPELKNIDYLLLLNGEED
                     YFDESAFIQLISKVPAIQTIIPIDVQSLKSKQNLLIRHFNEHHKKKNENSSYYWPGF"
     gene            complement(1988..2206)
                     /locus_tag="PLF48_11380"
     CDS             complement(1988..2206)
                     /locus_tag="PLF48_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49977.1"
                     /translation="MNPFSHYDLEKPVFEKELKDTLASVDKLKAVDIKALKKTTFDQL
                     NKEIDELKIKLTEKDNLVQTLNAKLKAK"
     gene            complement(2319..>2579)
                     /locus_tag="PLF48_11385"
     CDS             complement(2319..>2579)
                     /locus_tag="PLF48_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49978.1"
                     /translation="GTVYELYFPELIKKHKREIIQHLGELPRFTDKMSLPAGKADDEQ
                     KMNICKTVFNRLNDREHPVRVNLFYMSSIPEIRIIEGKTENN"
BASE COUNT          677 a          510 c          498 g          894 t
ORIGIN      
        1 gctctgcctt ttcctcgtat aggcatactg gttacactga tgacaaaatc tccatgatgg
       61 acttgttcat cctcttttag cttgtcaatc aaatcataaa tggtatcatc agtactttca
      121 aacttattat aataataagc atccacaccc caaaccaaac taagcgtatt caacagtgag
      181 ggattggatg tgaaaatatg tatgtcggat ttcgggcgat tgctggctaa catgaatgca
      241 gtatatcctg aataggtcat tccgtttatt gcttttgcgt tcacatcttc tgcaattttg
      301 catgcattat agcagatggc atcagaaata aacgtgtgcg agtttttatt gggcaaatga
      361 tgtttgttgt atatggaagt ttcttgctct gtacgtctga tgattttatc tatagtctgc
      421 accactttag ccggattttt tcctacagat gtttctccgc taagcataat ggcatcagca
      481 ccatccagta cgccgttggc aacatccgta atttcagcac gggtaggtcg cggcatttcc
      541 atcatcgact ccattacctg tgtcgctatg attaccggtt tggctttcag gatacattta
      601 tgtatcacac ttttttgtat caggggcatt tcttcaatgg gcaactcaac tcccaagtcg
      661 ccacgggcta ccattaccgc atctgatacc gaaataatgc tgtcgatatt ttcaatggct
      721 tccggctttt ctattttggc aatgagtttg gcatgcgcac gcattcggtg aatatgttcc
      781 ctcaactctt gtatgtcttc cgctttgcgt acaaaggata aggctatcca gttgacaggc
      841 tgctgtacga taaattctaa atcgcgcttg tctttttcgg ttaaactggg caaagaaatt
      901 tcggtattgg gcaggttgaa tccctttttt gaagaaagaa ttcctcccga aatgatttta
      961 cattctatcg ttttattgtc aattattttt gcaactttaa gttccagttt gccatcgtca
     1021 attaaaatcc attctccggg cttcaaatcc tgggcaagaa agtcgtaggt aaccgaaatc
     1081 aattctgacg tgccaattac ttcttcatta gtcacccgga tagtatgtcc ttcacgaagc
     1141 aaaaccgcat tgttttgcat caaacccgtt cgcattttgg gaccttgcaa atccgctaaa
     1201 attgcaacat gtgcatgcag attttcgttc agtttagtta tctgttctat cacctcgaga
     1261 tgatcttcat gtctgccgtg cgaaaaatta agacgaaaga cattgacacc gctttcatac
     1321 aattcaacca ggcgttcgta ggtattagaa gccgggccaa tagtagctac tattttcgtt
     1381 tttcttcttg tggtgttcat taaaatgtcg gattaacaga ttttgtttgg attttagcga
     1441 ttgcacatca atcggtataa tggtctgaat ggctggaact ttagaaatca gttgtataaa
     1501 tgctgattcg tcaaaataat cttcttcgcc attcagcaat aagagataat cgatgttttt
     1561 taattctgga atcagataat tacccgcatt tttattttgg ataaattcaa gcgtataaaa
     1621 atctatgacg ttgctgtagc tgtaagcatg aaaaaaactg aatgattttt gcttggtatt
     1681 gttgaattcg atatcagcga tgcgtttcat tttccagtgc agcaattcat taaggtgcca
     1741 gcaaatgcgg tagtcttcca tagcggagtt gatgccccac accgaaaagt caaaatacaa
     1801 atcaataatt tgccgcttag ccatttgggg taaaagtaca gccgcttttt atatttaaat
     1861 atgctagttt ttcaacaaat gtaagtaagt ggaatgtgga tggtttaaag aaatttgaat
     1921 ctaagcaaat ggatatacta tgatattttg atttgtgtgt ctatgggctg tgaactccca
     1981 tgaggtttca ttttgccttc agttttgcat taagtgtctg aactaaattg tccttttcag
     2041 taagttttat tttcaattcg tcaatttcct tgttcagttg gtcaaaggtt gtttttttta
     2101 gtgcctttat atcaactgct ttcagtttgt ctacagatgc taaggtgtct tttaattcct
     2161 tttcaaatac cggtttttca aggtcgtagt ggctgaatgg attcattaca actcccctgt
     2221 gaatcttgat ttctttaact aaagcagggg caaggttgat tagaggttaa aggactgctt
     2281 tcatttggtt gtaagtgata gctcgagact tttccatttt aattgttttc tgtttttcct
     2341 tcaatgattc tgatttcagg aatagaactc atgtaaaata aattcaccct caccggatgc
     2401 tcccggtcat tcaagcggtt gaaaaccgtt ttgcagatgt tcattttctg ctcatcatct
     2461 gccttgccgg caggcaggct catcttgtcg gtaaaccgcg gcaactcacc aaggtgctgt
     2521 atgatttccc ttttgtgttt tttaatcaac tctggaaaat acaactcata caccgtacc
//
LOCUS       DAOQII010000313         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_28987_length_2568_cov_3.664544, whole genome shotgun sequence.
ACCESSION   DAOQII010000313 DAOQII010000000
VERSION     DAOQII010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28987_length_2568_cov_3.664544"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLF48_11390"
     CDS             <1..1009
                     /locus_tag="PLF48_11390"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNF49979.1"
                     /translation="FDTTRANIEFTGAQAFDVIYEEAKDPQKELPFKGDLNVEEADAI
                     IQKFGADRIGAIVLTITNNSAGGQPVSYKNALAVRALCDKYGILLVLDACRIAENSYF
                     VKHREAGFENQTYREIGTAFLSLGDAFIMSAKKDGLVNMGGLLTLKDKKLAEQCMNLL
                     IISEGFVTYGGLSGRDMEALAVGLQEVFDDDYLHYRIKSTEYLGRHLNEMGVPTVKPY
                     GGHAIYVDAKAFYSHIPPYEYPGQALVCDLYVKGGIRCCEIGSVMFGKTDVQGNFVPA
                     QFELVRLAIPRRVYTQSHIDYVIDIFAELSEEKQASKGMKITYEPPFLRHFTAHFDRV
                     G"
     gene            1102..>2568
                     /locus_tag="PLF48_11395"
     CDS             1102..>2568
                     /locus_tag="PLF48_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNF49980.1"
                     /translation="MLQATNLSIIFGSRILFDNVTFTIQPKDRIGLVGRNGAGKSTLL
                     KTLNGEITPQEGSIAKPSSYSIGFLKQEIRAQNEPTVWLEAQTAFREINILQAKLDDI
                     QHQLETRTDYDTDDYLNLSQEFCDINDRLVHLDIDDKDKNTELVLKGLGYQREDFDKA
                     PSTFSGGWAMRLELAKLLLQYPDLLLLDEPTNHLDIESIIWLESFFKTYDGAIVLVSH
                     DKKFLDNVCNKTVELELGKMYEYKANYSKYLILKEERREKQLQTYKNQQDQIKQIERN
                     IERFKAKASKASFAQSLVKKLDKMDVVEVEQDDVRAMRFQFPMGQQSGKVVVKSTNLA
                     KSYGEKEVFHDVNLEIERGDKVAFIGKNGMGKTTMAKIIAKEINYDSGNLDLGHNVSR
                     AFFAQHHAEHLPKNLTILQYMEDTAPPETRAQVRNILGAFMFSGEDATKKISVLSGGE
                     RARVCLAHLLLHTSNVLILDEPTNHLDIKAKEVLKEAIS"
BASE COUNT          801 a          454 c          586 g          727 t
ORIGIN      
        1 ttttgatacg acacgtgcca atatagaatt tacaggtgcg caagcttttg atgtcattta
       61 tgaggaggct aaagacccgc aaaaagaact gccttttaaa ggcgatttga atgtagaaga
      121 agctgatgct attatacaaa aatttggtgc tgaccgaata ggtgctatcg tgttaacaat
      181 taccaataac tccgcaggcg gtcagcctgt aagttataaa aacgcacttg ctgttcgtgc
      241 attgtgcgat aaatatggca ttttgcttgt gttggatgct tgcagaattg ccgaaaacag
      301 ctacttcgtt aaacaccgtg aggcaggttt tgaaaatcaa acgtatcgtg aaattggaac
      361 ggcatttcta agtttgggcg atgcatttat tatgagtgcc aaaaaagacg gcttagtgaa
      421 tatgggtggt ttgcttacgc tgaaagataa aaagctggct gagcaatgca tgaatttgct
      481 gattatttca gagggatttg ttacatacgg cggtttaagc ggcagagaca tggaggcact
      541 ggctgtcgga ttgcaagaag tatttgatga tgattattta cactatcgca taaaaagtac
      601 tgaatatctg ggtaggcatt taaatgaaat gggagttcct acggtaaagc cttatggcgg
      661 acatgccatt tatgtagatg ccaaagcttt ttatagtcat ataccccctt acgaatatcc
      721 gggtcaagcc ctcgtctgtg atttatatgt aaaaggcggc atccgctgtt gcgaaattgg
      781 ttcggtgatg tttggcaaaa cggatgtaca aggcaatttt gtgcccgcac aatttgagtt
      841 ggtacgtttg gctattcctc gaagggttta cacacaaagt catatcgatt atgtgattga
      901 tatttttgct gaattgagtg aagaaaaaca agcttcaaaa ggcatgaaaa ttacatatga
      961 acctccattt ttgcgccatt ttactgccca ttttgatcga gtaggatagc tgatgtgctt
     1021 ttgtttgcaa tctgctacgg ctctgttgca tctgcacatt cttaaacttc ccgaaacaac
     1081 aatttcccta tttttgcatc catgttgcaa gctactaatc tttctatcat atttggcagt
     1141 cgtatcttat tcgacaatgt tacttttacc atacaaccca aagaccgtat cggtttggtg
     1201 ggcagaaacg gtgcgggaaa atctactttg ctgaaaaccc tgaatggcga aattactcct
     1261 caagaaggaa gtattgccaa gccgagttct tattctatcg gttttttgaa acaggaaatc
     1321 agagcgcaaa atgaacctac cgtatggctg gaagcacaaa ctgcatttcg tgaaatcaat
     1381 attttgcaag ccaaactgga tgatatccag catcagctgg aaaccagaac ggattatgat
     1441 acggatgatt atctgaattt atctcaagag ttttgtgata tcaatgaccg tttggtgcat
     1501 ttggatatag acgataaaga caaaaacacc gaactggtgt taaaaggttt gggctatcag
     1561 cgcgaagatt ttgataaagc gccatctact ttttcaggag gctgggcgat gcgcttagag
     1621 cttgccaaat tattgttgca atatcccgat ctgttgttgc tggatgagcc gaccaatcac
     1681 ttagacattg agtctattat ttggttggaa agtttcttta aaacgtatga tggtgcgata
     1741 gtactggttt cgcacgacaa aaaatttctg gataatgtat gtaataagac tgtcgagctg
     1801 gaattgggaa aaatgtatga atacaaagcc aattattcca aatacttgat attaaaagaa
     1861 gagcgacgag aaaaacaatt gcaaacgtat aagaatcagc aagatcaaat caaacaaatt
     1921 gaacgcaata tcgaacgatt taaagccaaa gccagcaagg cttcttttgc acaaagtttg
     1981 gttaaaaaat tagataaaat ggatgtggtc gaagtggagc aagatgatgt gcgtgccatg
     2041 cgttttcagt ttccgatggg acaacaaagc ggaaaagtag ttgtaaaatc cacgaatctg
     2101 gcgaaaagtt atggcgaaaa agaggtcttc catgatgtca accttgaaat agaacgtggc
     2161 gataaagtag cttttatcgg taagaacgga atgggcaaaa ccacgatggc aaaaatcatc
     2221 gctaaagaaa taaactatga ttcgggtaat ttagatttgg gtcataatgt gagccgtgca
     2281 ttttttgcac aacatcatgc cgaacactta cccaaaaatc tcactatact gcaatacatg
     2341 gaagatacgg caccacccga aacgagggca caggtgcgca atattttagg tgcgtttatg
     2401 ttcagcggtg aggatgccac caaaaagata agcgtactga gtggaggtga aagagcgcgt
     2461 gtatgccttg cacatttatt gctccataca agtaatgtgt tgattttgga tgagccgacc
     2521 aatcaccttg atatcaaagc taaggaagtg ctgaaagagg ccatttcc
//
LOCUS       DAOQII010000314         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_30510_length_2494_cov_4.361214, whole genome shotgun sequence.
ACCESSION   DAOQII010000314 DAOQII010000000
VERSION     DAOQII010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30510_length_2494_cov_4.361214"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLF48_11400"
     CDS             complement(<1..1064)
                     /locus_tag="PLF48_11400"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNF49981.1"
                     /translation="MKDQTQFFQNVSTFVDQAGIYTDFPKGLLNQIKVCNAVYQINFP
                     IRIGKDIQVIEAYRVQHSHHCLPTKGGIRYSEMVNQDEVMALAALMTYKCAVVDVPFG
                     GAKGGIKINPKQYTVEQLEQITRRYTFELIKKGFIGPALDVPAPDYGTGEREMSWIVD
                     TYIQFNAKEIDAHGCVTGKPISQNGIHGRSEATGRGVFYGLKQACSHAEDMKKIGLKP
                     GLQDKTVIVQGFGNVGYHSARVMQENGAIIVGIAEYEGGLYNPKGIDVEDLKKYQIEN
                     KTIRNYPKAKFIKDSNAILELECDILIPAALENQITTTNAPKIKAKIIGEGANGPVTP
                     AAENTLLKKGVLIIPDMYLNA"
     gene            complement(1079..1285)
                     /locus_tag="PLF48_11405"
     CDS             complement(1079..1285)
                     /locus_tag="PLF48_11405"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNF49982.1"
                     /translation="MFPKLQLSILMSLLTLSVFAQSNSIGDQLQNSNKLYAVIAVLCV
                     IFIGIVLFLISIDRKVKQLEKEIK"
     gene            1472..2461
                     /locus_tag="PLF48_11410"
     CDS             1472..2461
                     /locus_tag="PLF48_11410"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNF49983.1"
                     /translation="MNKNWMRFVLIAALCTSLNMLFAVDYANQANWAALPTKADNADW
                     VPENSGLENKQDSAQADVFYIHPTTDIAGFKGNANIDNERINKYTDNYPLKYQASVFN
                     GSCKVYAPRYRQAALNNFFARNSDRSQNSFDTAYADVKAAFAYYLKYYNRGRPIIIAG
                     HSQGTMLAKRLLQEFFDGKPLSSQLVEAYLIGYPTHENEFAVLKPSENPDDVGGYVSY
                     NTFGENAKTDWMKEYKDAVCVNPLSWKQDEVFVSSKEQKGALKKGDDELIKNSFGCKC
                     SDGILEIQKPDVKGFTTIGFKNYHLYDYGLFYLNIRENVALRITRYLKKENTK"
BASE COUNT          750 a          474 c          467 g          803 t
ORIGIN      
        1 gcatttaaat acatatccgg aataatcaat acacctttct tcaacaatgt attttctgca
       61 gcaggcgtaa ccggaccatt agcaccctca ccaattattt tagccttgat tttcggcgcg
      121 tttgtcgttg taatctggtt ttctagggca gcaggtatta atatatcgca ttccagttct
      181 aaaatggcat tagaatcttt tatgaattta gcttttgggt aattgcgtat ggttttgttt
      241 tcgatttgat attttttcaa atcttctaca tcaattcctt taggattata aagccctcct
      301 tcatactcag ctatacccac tataattgca ccattttcct gcattactct tgcactgtga
      361 tagcccacgt taccaaaacc ctgaacgatt acagttttat cttgtaagcc cggttttaat
      421 ccaatttttt tcatgtcttc tgcatgagag caagcctgtt tcagtccgta gaaaacacct
      481 cgtccggtcg cctcacttct gccatgaata ccattttgtg aaatcggttt tccggttaca
      541 caaccatgag catcaatttc tttggcatta aactgtatat aagtatcaac aatccagctc
      601 atttcacgtt cacccgtacc ataatccgga gcaggcacat ccagtgcagg tccgataaac
      661 ccttttttga ttagttcaaa ggtatatctt ctggttatct gctccagttg ttctacggta
      721 tattgctttg gattaatttt gataccgcct ttggcaccgc caaatggcac atctactacg
      781 gcacatttat aagtcattaa agcagcaaga gccatcactt catcctgatt taccatttcg
      841 ctgtagcgaa tacccccttt ggtaggcagg caatgatggc tatgctgtac tctataggct
      901 tctataactt gtatatcttt tccgatgcgt atcggaaaat tgatttgata aacagcatta
      961 catactttga tttgattgag gagacctttg gggaaatctg tataaattcc ggcttggtct
     1021 acgaatgtag atacattctg aaaaaattgg gtttgatctt tcatactact ttttataact
     1081 actttatttc tttctctaat tgtttgactt tgcggtctat gctgattaag aacaatacta
     1141 tgcctataaa tataacacaa agtacggcta taacagcata aagtttgttg ctgttttgca
     1201 gctggtcgcc gattgagttg gactgagcaa atactgaaag tgtaagtaaa ctcatcaaaa
     1261 tggacaattg taatttcggg aacattctaa ctaattttaa attaatattt tgcgggtgta
     1321 aaattaacgt aagatattga tatgtacaat agataaaaat aaaggaagtc aaatatcatt
     1381 aaatataggg aatgggctat tttatcattt ttacatcaag tttcaggcat aattcttgta
     1441 atttttcgtt ttattagcaa aatatttaaa gatgaacaag aattggatgc gatttgtgct
     1501 gattgctgct ttatgcacaa gcctgaatat gctgtttgcc gttgattatg ccaatcaggc
     1561 aaactgggcg gctttgccta caaaagctga caatgccgac tgggtgcctg aaaattcggg
     1621 cttggaaaac aagcaggatt cggcgcaagc agatgttttt tatattcacc ctactaccga
     1681 tattgccgga tttaaaggca acgccaacat agacaatgaa cgcatcaata aatacaccga
     1741 taattatcca ttaaagtatc aggcatccgt gtttaatggc agttgtaagg tatatgcacc
     1801 tcgctatcgt caggcggcat tgaacaattt ttttgcccga aattcagacc gctctcaaaa
     1861 ctcattcgat acggcgtatg ccgatgtcaa agctgccttt gcatattatc tgaagtatta
     1921 taaccgcgga agacccatta ttatagcagg gcactcgcaa ggcacgatgc ttgccaaacg
     1981 attacttcaa gaattttttg acggcaagcc attaagcagt cagcttgtag aggcttatct
     2041 tattggctat cccacgcatg aaaatgaatt tgccgttttg aaaccctctg aaaatccgga
     2101 tgatgtcggt ggctatgtat cttacaatac ttttggtgaa aatgccaaaa cagactggat
     2161 gaaagagtat aaagatgcag tctgtgtgaa tcccttaagt tggaaacaag atgaggtttt
     2221 tgtatcctcc aaagaacaaa aaggcgcatt aaaaaagggc gatgatgaat tgataaaaaa
     2281 ttccttcgga tgtaaatgca gtgacggtat attagaaata caaaagccgg acgtaaaagg
     2341 gtttacgact atcggattta aaaattatca tttgtatgat tacggactgt tttatttgaa
     2401 tattcgggaa aatgtagcat tgcgaattac ccgctattta aaaaaggaaa atacaaaatg
     2461 atagttataa aaacagaacg gctttttatt agtg
//
LOCUS       DAOQII010000315         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_31354_length_2455_cov_5.375000, whole genome shotgun sequence.
ACCESSION   DAOQII010000315 DAOQII010000000
VERSION     DAOQII010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31354_length_2455_cov_5.375000"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLF48_11415"
     CDS             <1..223
                     /locus_tag="PLF48_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNF49984.1"
                     /translation="YQKRAKVNEAKNISDAQKGVEMVVENLTGIILLERTILPVAEEL
                     NDEGTITMLTDFITQQEKEIWMYTAWLAH"
     gene            293..838
                     /locus_tag="PLF48_11420"
     CDS             293..838
                     /locus_tag="PLF48_11420"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNF49985.1"
                     /translation="MALMNVGQKFPDFKKKAVVSLEQGKEFQEVDNSIHTKDGKWLVM
                     YFWPKDFTFVCPTEIVEFDKAHGEFKDRDAVLLGGSTDSEFVHLGWRNANADLKNLTH
                     PMVADTSKSLARELGILTGGDEVCYRATFIVDPQGIIRWVAVNDLSVGRNVKEVLRVL
                     DALQTDELCPCNWEKGQKTLS"
     gene            926..1501
                     /locus_tag="PLF48_11425"
     CDS             926..1501
                     /locus_tag="PLF48_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF49986.1"
                     /translation="MHETILELLAELGIPADYQNNVLLHLGAADSKYLRDLKLNVKAV
                     LQSEHINPKEAYLLALAIATNADNTVLSQAFSAKAGEHGASEAEIAEAQACASLLSAN
                     NVIYRFRHFVSKEVYDNAPARLRMNIMMNPVLGKEFFELISLAVSAVNGCEMCVKSHE
                     QSVLHAGGTELRIWDAIRLASIIQSLHKVIF"
     gene            1752..>2455
                     /gene="thiL"
                     /locus_tag="PLF48_11430"
     CDS             1752..>2455
                     /gene="thiL"
                     /locus_tag="PLF48_11430"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNF49987.1"
                     /translation="MEKTKRTEISALGEFGLIDRLTQDILTNHTSTVKGIGDDAAVLH
                     PHKTQVITTDILVEGIHFDLVYTPLKHLGYKSVVVNLSDVYAMNAIPKQILVTLAVSN
                     RFSVEALEELYAGVKLACAHYQVDLVGGDTTASQKGLIINVVAIGEQDQDKIAYRNGA
                     KEGDLLCVSGDLGAAYMGLNLLQREKQVFLNAPEAEIQLSGDEYIVGRQLKPEARKDI
                     IEFFAENQLVPTAMID"
BASE COUNT          782 a          431 c          539 g          703 t
ORIGIN      
        1 ttaccaaaaa agagctaaag taaacgaagc aaaaaacatc agtgatgcgc agaaaggcgt
       61 ggaaatggtg gtggaaaatc ttacaggtat tattctgttg gagcgtacca ttttgcccgt
      121 tgcagaagaa ctgaacgacg aaggtacgat taccatgttg accgatttta ttacccaaca
      181 agaaaaagaa atctggatgt acaccgcttg gttagcacat taatttttat gtgcttcgta
      241 taaaattgca gatatttgta ttctaatttt taatccttta aataataaaa aaatggcatt
      301 aatgaatgtg ggacaaaagt tccctgattt taagaaaaaa gcggtagtaa gtcttgaaca
      361 aggtaaagag tttcaagaag tggacaacag catccacacc aaagacggaa aatggctggt
      421 gatgtatttt tggccaaaag attttacgtt tgtttgccct accgaaatcg tagagtttga
      481 caaagcgcat ggcgaattta aagaccgtga tgctgtactt ttaggtggtt ctaccgacag
      541 cgaatttgta cacttgggtt ggagaaatgc taatgcggac ttgaaaaacc ttactcatcc
      601 tatggtagct gatacttcta aatcccttgc ccgcgaattg ggtattctta ccggtggcga
      661 tgaagtttgc tacagagcta catttatcgt tgacccacag ggcatcatcc gttgggtagc
      721 tgtaaatgat ttgtcagtag gacgcaatgt aaaagaagta ttgcgtgtac tagatgcatt
      781 gcaaacagat gaactttgcc catgcaattg ggaaaaagga caaaaaacat tatcataaac
      841 tcaggcaata agtataaagc agtaagtagt taggctgctg ctttatacta tttttcccat
      901 accacttttt acttatacct atatcatgca cgaaactata ttagaactgc tggcagaatt
      961 aggcattccg gcagattatc aaaacaatgt cttattgcat ttgggtgcgg cagattccaa
     1021 atacctccgc gatttaaaac tcaatgtaaa agccgtttta cagtcggaac atattaatcc
     1081 gaaagaagct tatttgctgg cattggcaat tgcgactaat gctgataata cagtattatc
     1141 acaagcattt tctgcaaaag ccggagagca tggagcttct gaagcagaaa ttgctgaagc
     1201 ccaggcttgt gcatcgttat tgtcggcaaa caatgtaatc tatcgttttc gtcattttgt
     1261 aagtaaagaa gtgtacgaca atgcgcctgc tcgtttgcgc atgaatatta tgatgaaccc
     1321 ggtattggga aaagaatttt ttgaactaat cagtttggcg gtaagtgcgg taaacggctg
     1381 tgaaatgtgt gtaaaaagcc atgagcaatc tgtcttacat gcaggcggaa cagaactccg
     1441 catttgggat gccatccgtt tggccagcat catacaaagc ctccacaaag tgattttctg
     1501 attttttacc catatcattt attttttagt ccttccttca ataccttcta atagtaatct
     1561 ggattcgggt taagaaaatt aaggtgaaaa aaatagttga tttctcctat tttcctccaa
     1621 cttaacaagg aggaggaatt aaaaggtggt ggttctatat caaaaatcaa ctatttttta
     1681 attgaaacgc ttaacccgaa ctcagattaa tagtatattg aaaacgcagt aggtattagt
     1741 atctttgcga catggaaaaa acaaagcgca ccgaaatttc ggcgttggga gagtttggtt
     1801 taatcgacag gctcactcaa gatattttaa caaatcatac ttctactgta aaaggaattg
     1861 gcgatgatgc cgccgtactt catccgcata aaacacaagt aattaccaca gatattttag
     1921 tggagggcat ccactttgat ttggtttata ccccgttgaa acacttgggc tacaaaagtg
     1981 tagtggtaaa tttgtcggat gtatatgcca tgaatgccat acccaaacaa attttagtaa
     2041 cgctggctgt cagcaatcgc ttttctgttg aagcactgga agaattatat gcgggtgtta
     2101 agctggcatg cgctcactat caggtagatt tagtgggggg tgatacgaca gcatctcaaa
     2161 agggtttaat aataaatgtg gtagctattg gcgaacaaga tcaggataaa atcgcataca
     2221 gaaacggtgc caaagaagga gatttgctct gtgtttcggg cgatttgggg gcggcttata
     2281 tgggattaaa cttattgcaa cgcgaaaaac aagtattttt aaatgcgccc gaagcggaga
     2341 tacagctgag tggcgatgaa tatattgtag gcagacagct aaagccggag gccagaaaag
     2401 acatcattga gttttttgca gaaaatcagc ttgtgccgac tgcaatgata gatat
//
LOCUS       DAOQII010000316         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_31431_length_2452_cov_4.267000, whole genome shotgun sequence.
ACCESSION   DAOQII010000316 DAOQII010000000
VERSION     DAOQII010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31431_length_2452_cov_4.267000"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..494
                     /locus_tag="PLF48_11435"
                     /pseudo
     CDS             141..494
                     /locus_tag="PLF48_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            504..1091
                     /locus_tag="PLF48_11440"
     CDS             504..1091
                     /locus_tag="PLF48_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49988.1"
                     /translation="MGAGISIWLVLFIWFGTLFYLKKFQKKTSSKKIPYSSQYNRKTA
                     IWWLALISIVVLFFGILLESTSDAIAAHFNLSGVFFGATFLAFVTSLPEISSGLKFVK
                     NKDYQPIIDDIFGGNGFLPVLFLPASMIAGKNIIGNAGSSNNLLSFLSIVLTLIFLLG
                     MWRKAPKKLGRLGWDTWLMLLAGIVGFAFLYRMTD"
     gene            1463..2173
                     /locus_tag="PLF48_11445"
                     /pseudo
     CDS             1463..2173
                     /locus_tag="PLF48_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
BASE COUNT          777 a          411 c          466 g          798 t
ORIGIN      
        1 atttacaaga ttgctaaccc cttggagaaa cacatctgat tttttgcatt tgctttatct
       61 tcactaattg tatttaaatg tcaaagttat gcatttaata tcctcattaa ctggtcaaat
      121 tgtgattttt ttttgtttca gccattgcca tctggtattt ctgcaataag ctgtccgatg
      181 tagtagaata catagatgca gaatttggtt taggtgctgc tttcggtggt actttaatct
      241 tatctgttgt tacgaatctt ccagaaattg ccatatctgt tacaggtgca attaaaggca
      301 actatgattt agtgacaggt aatctgttgg gtggaattgc catgcagagc atgctattaa
      361 tcatgtacga ttttgccagt aaagatgtgc gacctctatc aacaatcacc tcttcacaag
      421 caggcagatt ccaaggactt acactaatag ccattcttac gatatgtttg ttaggtggga
      481 tctttaaaga gaaaactact cttttgggtg caggtatttc tatctggctc gttttattca
      541 tctggtttgg cacactgttt tatttaaaaa aatttcagaa aaaaacgtct tcaaaaaaaa
      601 taccgtacag ctctcaatac aatagaaaga cagctatttg gtggctggca cttatctcca
      661 ttgtcgtatt gttttttggg attttgctcg aaagcaccag tgatgccatt gctgcccatt
      721 ttaatttatc gggtgtattt ttcggtgcta catttttagc tttcgtcact tccctacctg
      781 aaatttcaag tggtttgaaa tttgttaaaa acaaagatta tcaacctatc atagatgata
      841 tttttggcgg taacggattt ttgcccgtgt tatttcttcc cgcttccatg atagccggaa
      901 aaaatattat aggaaatgca ggaagcagta ataatttgct ttcattcctt tctatagtgc
      961 tgacacttat ttttttatta ggcatgtgga gaaaagcacc aaaaaaatta ggtcgtttag
     1021 gatgggatac ctggctcatg ttgctggcag gtattgttgg atttgccttc ctttatcgca
     1081 tgacagatta aaaaataaat ttaatattaa tagtatgaaa cttatcagca ttagtttagc
     1141 agctggaatt cttttggcta cagcttgtaa aaacaaggcg gaaaatgaac atgaaagaat
     1201 tgggtacagc ggtacagatc taagtagcct agcatccatt tcgagagata aagaaacaaa
     1261 gaaagctata tttgaaacat ccttgaaaga agagtggagt ttatatgccg gactttcacc
     1321 ggattctatt gatttcacca aacccttgct atccggtaaa actagcggaa catttccatt
     1381 ggatgtatcg gcaaataaac gctattattt tgaattggtt acaaaagaaa gtagtgcagc
     1441 actcgcagaa aggcatctgc ccatgagcgg aggattcaat tttcgggatc tgggtggcta
     1501 caaaacaaaa gaaggtaagt ttgtgaaatg gggtaaaatc attcgttctg atgatttaag
     1561 caagttgacc gatgctgatt tggaatattt aagttcaatg ccactcctaa ccgatattga
     1621 ttatcgttcc gtggaagaaa tcaaaccggc tccggataaa cgaccacaat cattattgaa
     1681 ctatctggaa tacaatattt ctccgggaaa taccgatgcg atgatgacca agttataaac
     1741 agcaaccagt gcagcaacag actcggaaat gatggcgatg aatgtatttt ttgtcacaaa
     1801 tgatgctgcg acaattcagt accaaaaaat gtttgaacac ttgcaaaatc cggagggtaa
     1861 tgttcccttg ctctaccatt gtacagctgg aaaagaccgt acaggtatgg cttccgcact
     1921 tatcttatat gcacttggcg cagatgataa aactgtaatg gatgactact tattgtcgaa
     1981 taaatatatt gaagcaaaat ttgagagtta tatagaacaa tatccaaact tattgggact
     2041 tttttcagta aaacaagaat acctgcaagc cggattagat gccattaata aaaactatgg
     2101 caatgtagaa aattttttag taaagaaatt aaatgttgat attgaaaaaa tgcacgaatt
     2161 gtatttatac tagtaataca tatactgttt aatattaaat tatatattta aatcatcatt
     2221 aactcatgaa cttatcccga tatagttggg agtgttcttt atatattggt aacaacaatt
     2281 tttaacagtt gatcaatata ataatcgttg tgaattgaac taatcccgac aaatcgggag
     2341 tgttctttgt atattggtaa caacaggcaa attttctata taatcgtcat caccgttgtg
     2401 aattgaacta atcccgacaa atcgggagtg ttctttgtat attggtaaca ac
//
LOCUS       DAOQII010000317         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_31894_length_2430_cov_4.773895, whole genome shotgun sequence.
ACCESSION   DAOQII010000317 DAOQII010000000
VERSION     DAOQII010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31894_length_2430_cov_4.773895"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PLF48_11450"
     CDS             complement(<1..1189)
                     /locus_tag="PLF48_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNF49989.1"
                     /translation="MNSLLFKSIWESIKSNFTISILLLTGIVLSCKKEIVTEAPKITD
                     TWQIQWVNVNQEQIVDEKARTLQLRGINVRVNGLFDVEHSDGRRANEIIPEITQRDIQ
                     DIAKMGFNFVRLPINWSGYEPEKDQFSTTYLNRIKEVVSWCKQENLYVQLDWHFDAYS
                     KEIGEDGAPYWAVLPTIFPKVEGILDFNQLFLLRANPFCFLAYRNFFSNTSGIQDEFF
                     TCWTNIIEAFKDENAIIGFEPMNEPICYAAGMSDAVFMDFYKKCSQKMRAIDSRHLLW
                     LEPDAIRNFLNLSPLPTQQFPDTKIVYCPHYYPNLTAGASYNQVAGWKSIMKTPMDRI
                     VEEAHAWHAPVCLNEWGINPSTVPGKAYIPAMREMAEARNIHQAFWVWKEPLPGTSGK
                     DGNW"
     gene            1230..>2430
                     /locus_tag="PLF48_11455"
     CDS             1230..>2430
                     /locus_tag="PLF48_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNF49990.1"
                     /translation="MKKYLVYCFIVCTQTVFGLDKNTLTDWTYAGKHTSYNNKTVSLA
                     IKDFGGIADNKTNNATALNKAIAALNGKPGTIEFGSGFFLFTQPVSLPSFVKLVGQGS
                     DKTSLNFNLNGNSDAISVQGKPDTISSKIINGTQKESTQITVENAGNFAVHDYCIIKQ
                     NASNLLNNDWAYNSFYQIVEITAIKGNTISIRSPMHLSFPAQPVLIKINPATDVSIEN
                     LTIKRMDITNAQTSTIFFNFAVNCNVSGVELSNSNYALINLQSSAFCTISGNYLHHAF
                     DYGSGGKGYGVVMQFGATDNLIIDNIAEHLRHSFLLQASSNGNVIAFNYSFDPYWKQG
                     FFPSGAAGDIVLHGNYPYGNLFEGNIIQNLVIDNSHGINGPNNTFFRNRMESYGIFMN
                     GNSGDNMN"
BASE COUNT          758 a          455 c          441 g          776 t
ORIGIN      
        1 cccagttgcc atctttgccg gatgtacccg gcagtggttc tttccacacc caaaatgcct
       61 gatgaatatt acgtgcttct gccatttccc gcattgcagg tatatatgct tttccgggta
      121 ctgtagacgg atttataccc cattcgttta ggcaaactgg agcatgccag gcatgagctt
      181 cttccacaat tctgtccatc ggtgttttca taatgctttt ccatcccgcc acttgattgt
      241 aacttgctcc tgctgtcaaa ttgggataat aatgcgggca ataaacgatt ttagtatcag
      301 gaaattgttg tgtaggcaat ggagataaat taaggaaatt acgaatggca tccggttcta
      361 gccaaagcaa atgccgggaa tctattgctc gcattttctg tgagcatttt ttataaaaat
      421 ccataaaaac agcatcactc attccagcgg catagcagat aggctcgttc atgggttcaa
      481 aaccaatgat tgcattttca tctttaaaag cctcaatgat atttgtccaa caagtaaaaa
      541 attcatcttg tatgccactc gtattactga agaaattgcg gtaggctaaa aaacaaaaag
      601 ggttagcccg taataaaaaa agctggttaa aatccagaat gccttctact tttgggaata
      661 tggtgggcaa cactgcccag tatggtgcac catcctcccc tatttctttg ctgtaggcat
      721 caaaatgcca gtcaagctgc acatacaaat tttcttgttt acaccaactg acaacttctt
      781 ttattctgtt taagtatgta gttgaaaact gatctttttc cggctcataa ccactccagt
      841 taatcggcag acgaacaaaa ttaaagccca ttttggctat gtcctgaata tcgcgttgag
      901 ttatttccgg tataatttca tttgcccttc tgccatcact gtgctccaca tcaaacaatc
      961 cattcactct cacatttatt ccacgaagtt gcagtgttct tgctttttcg tctacaattt
     1021 gttcctgatt gacatttacc cattgtattt gccatgtatc agtaattttg ggtgcttctg
     1081 taacaatttc ttttttgcag gataaaacaa ttcctgtcaa taaaaggata ctgatagtaa
     1141 aatttgattt tatactttcc caaatcgatt taaataaaag agaattcatt acttggtata
     1201 caatttgagt aaatttacaa aattagtgta tgaaaaagta tttagtatat tgttttattg
     1261 tatgtacgca gactgttttt ggattggata aaaatacatt gactgactgg acttatgcag
     1321 ggaaacacac gagttataat aataagacag taagtttagc tatcaaagac ttcggaggga
     1381 ttgcagataa caaaaccaat aatgcaactg cactgaataa ggctatagca gccttaaatg
     1441 gcaagcccgg aactatcgaa tttggtagcg gcttcttcct atttacacag cctgtaagtc
     1501 tgccttcatt tgtaaaatta gttggtcagg gaagtgataa aaccagctta aatttcaatt
     1561 taaacggaaa ttctgatgca atcagtgtac aaggcaaacc agacacaatc agctctaaaa
     1621 taattaacgg tactcaaaaa gagagcacgc agataactgt agaaaatgcg ggtaattttg
     1681 ccgttcatga ttattgtatt atcaaacaaa atgcatccaa tttactgaac aatgattggg
     1741 cttataattc attctatcaa atagtagaaa ttacagccat aaaaggtaat acaatttcta
     1801 ttcgttcgcc tatgcatctt agttttccgg cacaaccggt attaatcaag attaatcccg
     1861 caactgatgt aagtatagaa aatctgacaa taaaacgaat ggatattacg aatgctcaaa
     1921 cctctacaat atttttcaat tttgctgtca actgcaatgt aagtggagtg gagttatcga
     1981 atagcaatta tgcactgatt aacttacaga gttcggcttt ttgtacaatt agtggcaatt
     2041 atctgcacca tgcttttgac tatggcagtg gtggaaaagg ctatggcgtc gtaatgcagt
     2101 ttggagcaac agataatttg attatcgata atattgctga acatttaaga cattcattct
     2161 tattacaggc cagttccaat ggcaatgtta ttgcattcaa ttactcattt gacccttatt
     2221 ggaaacaagg atttttccca agtggtgctg ccggtgatat tgttttacat ggcaattacc
     2281 cgtatggcaa tttatttgaa ggcaatatca ttcaaaatct tgttatagat aattctcatg
     2341 gaatcaacgg acctaataat acgtttttca gaaaccgtat ggagagttat ggaattttca
     2401 tgaatggaaa ttcgggagat aatatgaatt
//
LOCUS       DAOQII010000318         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_32604_length_2401_cov_4.667093, whole genome shotgun sequence.
ACCESSION   DAOQII010000318 DAOQII010000000
VERSION     DAOQII010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32604_length_2401_cov_4.667093"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLF48_11460"
     CDS             <1..1073
                     /locus_tag="PLF48_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF49991.1"
                     /translation="FPVGEEDIAEVVAMMTGIPVKRVAQSESKKLVEMAKDLGKQVIG
                     QSEAVEKITKAIQRNRVGLKDPKKPIGTFIFLGPTGVGKTELARALSRYMFDTEDSLI
                     RIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTERVRRKPYSVILLDEIEKAHPDVYN
                     ILLQVLDDGQLTDGLGRKVDFKNTIIIMTSNIGVRQLKDFGTGVGFATSAIKDNQDNI
                     EKGVIQKALQKTFSPEFLNRIDDVVIFNSLGKEEIFKIIDIVLSGVYKRLENLGFQIE
                     LSDAAKGFLSEKGYDPQFGARPLHRAIQKYVEDPLAEEILNSGVQEGDLIVVDTNPDD
                     KEKLKFEIRRKSKPKKAKEEQN"
     gene            1100..1357
                     /locus_tag="PLF48_11465"
     CDS             1100..1357
                     /locus_tag="PLF48_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49992.1"
                     /translation="MTPEEEKGFFDKLKDTAAEKLEQLKETAEELKDKAAEKLEDLKE
                     GAEDLKEQAEGKFNELKDKATDAIEDLTSKKEDTTETSAKE"
     gene            complement(1448..2098)
                     /locus_tag="PLF48_11470"
     CDS             complement(1448..2098)
                     /locus_tag="PLF48_11470"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNF49993.1"
                     /translation="MEFIHFLLELLTNTKEVLLHLFQQYGTYFYVIVFAIIFIETGLV
                     IMPFLPGDSLLFTAGMLCASGTGLKIYYLIPLLIFAAILGDNVNYFVGRFFHEQVLKL
                     QFRGKPLVKQQWLDDAHAFFEKQGTKTIILARFVPIARTVVPFVSGFSEMNYKTFLPY
                     DVVGGILWVSSVTIVGYFLGTVPFVEQHLEKFIIGIVFVSVIPLFYKAIQAKFSKK"
     gene            complement(2190..>2401)
                     /locus_tag="PLF48_11475"
     CDS             complement(2190..>2401)
                     /locus_tag="PLF48_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF49994.1"
                     /translation="STIKSITAKKELITVMIAHRLSTIMHADRIYVLEKGKIIETGKH
                     YELLEQKGLYYAMWRQQIGERKSRE"
BASE COUNT          857 a          418 c          486 g          640 t
ORIGIN      
        1 gtttccctgt aggcgaagaa gatattgcag aagtagtagc gatgatgaca ggcattccgg
       61 taaaacgtgt tgctcaaagc gaaagcaaaa aactggtgga gatggctaaa gacttgggca
      121 aacaagttat tggtcaaagt gaggccgtag aaaaaattac caaagcaata caacgcaaca
      181 gagtgggtct gaaagacccg aaaaaaccaa taggaacttt cattttttta ggccctaccg
      241 gtgtaggtaa aaccgaatta gcccgtgcct tatctcgcta tatgtttgat acggaagatt
      301 ctttaatccg cattgatatg agtgagtata tggagaaatt ttccgtatct cgtttgatag
      361 gtgcacctcc gggatatgta ggctatgagg agggcggaca attaacagaa agagtacgcc
      421 gtaagccata ttctgtcatc ctgttagacg aaatagaaaa agcacatccg gatgtataca
      481 atatcttatt gcaagtattg gacgacggac agctgacaga cggattaggc aggaaagttg
      541 atttcaaaaa tacaatcatc atcatgacct caaatattgg tgttcgtcaa ttgaaagatt
      601 tcggtacagg tgtcggattt gccacatcag caattaaaga taaccaagat aatattgaga
      661 aaggagtaat tcaaaaagca ttacaaaaaa ctttctcccc ggaatttttg aatcgtatcg
      721 atgatgtggt tattttcaac agtttgggca aagaagaaat cttcaaaatt attgatattg
      781 tgttatcagg tgtgtataag cgattggaga atttaggatt ccaaatagaa ttgagtgatg
      841 ctgcaaaagg attcttgagt gagaaaggct acgatccgca gtttggtgca cgtccgctgc
      901 atcgtgccat acaaaaatac gtagaagatc cgctggcgga agagatactg aacagtggcg
      961 tgcaggaagg ggatttaatt gtagtagata cgaatccgga cgataaagag aaactgaaat
     1021 ttgagattag aagaaaatct aagcctaaaa aagctaaaga agaacagaat taattattca
     1081 cctataaaaa acgtacatta tgacaccgga agaagaaaaa ggatttttcg acaagctaaa
     1141 agacacggct gctgaaaaat tagagcaatt aaaagaaaca gctgaagagc tgaaagacaa
     1201 agctgctgaa aaattagaag atctgaaaga aggagctgaa gatttgaaag agcaagcaga
     1261 aggcaaattc aacgagctga aagacaaagc aacagatgct attgaagatc tgacttctaa
     1321 gaaagaagac actacagaaa caagtgctaa agaataattc tttaagactg aaattagagg
     1381 cggggcgctt cgcgccccgc tttcttttta caaatgaatt cttgcaaatt ccaaaatata
     1441 ttttgagtta ttttttactg aacttagcct gtatcgcttt ataaaacaac gggatcactg
     1501 aaacaaaaac aataccgata atgaactttt ccaaatgttg ttcaacaaac ggcacggtgc
     1561 ctaaaaaata acccactatt gtaacactgc tcacccacaa aataccgccc acaacgtcat
     1621 aaggcaaaaa agttttatag ttcatctcgc tgaagcctga aacaaaaggc actacggtgc
     1681 gtgcaatcgg aacaaaacgg gctaaaataa tcgtcttagt tccctgtttt tcaaagaaag
     1741 catgtgcatc atccagccat tgctgtttaa ccaagggctt gcccctgaat tgcaatttca
     1801 atacctgttc gtgaaaaaac ctgcccacaa aatagttgac attatctccc aaaattgccg
     1861 cgaaaataag taacggaatc agataataaa ttttgagtcc tgtgccactg gcgcaaagca
     1921 taccagctgt aaacaataaa gaatcgccgg gcaaaaacgg cataatcacc aagcctgttt
     1981 ctataaaaat aatggcaaat acaattacat aaaaatatgt gccgtattgc tgaaataaat
     2041 gcaataatac ttccttagta ttggtgagta gttcgagtaa aaaatgtata aattccataa
     2101 attttaataa cgtgcaaaat acactttttt tatgaagtat aacatatttt gaattaagat
     2161 atatttattg tattgtgtaa agtgaatttt tattctctgc ttttgcgttc gccaatttgt
     2221 tgccgccaca tagcatagta caaacctttt tgttctaata attcataatg tttcccggtt
     2281 tcaataatct ttcctttttc taatacataa atacggtcgg catgcataat agttgacaaa
     2341 cgatgggcaa tcatcacggt gataagctct tttttggcag tgatgctttt tatggtagaa
     2401 g
//
LOCUS       DAOQII010000319         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_34391_length_2334_cov_4.715665, whole genome shotgun sequence.
ACCESSION   DAOQII010000319 DAOQII010000000
VERSION     DAOQII010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34391_length_2334_cov_4.715665"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..491)
                     /locus_tag="PLF48_11480"
     CDS             complement(30..491)
                     /locus_tag="PLF48_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49995.1"
                     /translation="MKKILFLIFLVFSIILTGHAQNKQKTVSKNSKQKTVQAKIKTDK
                     MPIDSLLFILKHCKKLVVNHQTEFMHIDVPVSKQGVFFGMIMDSVALPSAEAKNLLYY
                     NITSDEGKVTNGDIYFLPDSAYIVFTLSGRKYYNKLTTDGMNQLKGFFKLK"
     gene            636..1883
                     /locus_tag="PLF48_11485"
     CDS             636..1883
                     /locus_tag="PLF48_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF49996.1"
                     /translation="MNTKANIDPYQALRYREFRYFLLARFFITVALSMQAVTIGYEVY
                     ELTHSKLLLGLIGLTEAIPAITIAMYGGYVADKSEKRTLLATSVSLFTLMCVGLLIVT
                     YPKILALCGNNITVKIIFVIIFITGIARSFSGPASFGLYAQIVPKEVYQSAITWSSSA
                     WQLGAVLGPACGGLILGIFGITATFACIVFFLIIAVMLILLIDKKPVLFVPQNESVYQ
                     NAIQGLKFVFEKKEILACISLDLFAVLFGGAVALLPVYAKDILHVGAQGLGWLRAAQA
                     MGAIVVLLLLAYHPIKKQAGIKLLLAVFGFGVFIILFGISRNFYFSMFCLIMSGALDG
                     ISVAVRHTIIQLATPDEMRGRVSAVNSMFIGSSNEIGEFESGLTAHWMGTVPAVIFGG
                     CMTCLIVAGTYIFSPRLRKLELK"
     gene            1946..>2334
                     /locus_tag="PLF48_11490"
     CDS             1946..>2334
                     /locus_tag="PLF48_11490"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNF49997.1"
                     /translation="MSDLLFTYGTLRLNQSHPMANYLAENATLVGLALLPKARLYKVD
                     WYPALTFTDNENDMVVGDVFSLNNLDALEKLDDYEGIGNGEIPYEYRREKVEIILEGN
                     KTACWVYFYNHPLPENAEWIASGDFLNP"
BASE COUNT          660 a          401 c          463 g          810 t
ORIGIN      
        1 ctttcctcaa agataaagat atatattgct tattttagtt taaaaaaacc tttcagttga
       61 ttcatgccat cagtagtcag cttattataa tattttcttc ccgataaagt gaaaacgatg
      121 taagcgctgt caggcaaaaa ataaatatct ccattcgtaa cttttccttc atctgatgtt
      181 atattgtaat acaataaatt ttttgcctca gccgatggca aagctaccga atccattatc
      241 attccgaaaa aaacaccttg cttagacacc ggcacatcaa tatgcataaa ttccgtttgg
      301 tgattaacaa ctaatttctt gcaatgtttc aagataaaga gtaaagaatc tatcggcatt
      361 ttatccgttt ttatcttggc ttgtactgtc ttttgtttgc tgtttttaga aacagttttt
      421 tgcttgtttt gggcatgtcc ggtcaaaata atactaaaca ctaagaaaat aagaaataga
      481 atctttttca tagacataaa tgtaggaaaa ctatttcatg cttcactact ccatgttatg
      541 ctttattaga atttggcttt ttttaagaaa actgtaatct ggtttgatat tttgagtcag
      601 aattattccc ttatttttac gcttagaact tttaaatgaa tacaaaggca aatatagatc
      661 cctatcaggc attgcgctat cgcgagtttc gttatttttt attggctcgg ttttttataa
      721 ccgtagcact ctccatgcaa gctgtcacga ttggttatga agtgtatgaa ttaacgcata
      781 gtaaattatt gctggggcta attggtttaa ctgaagccat tccggcaatt accattgcca
      841 tgtatggcgg ttatgtggct gataaatcag agaaaagaac tttgctcgca acgtctgtct
      901 ctctttttac attgatgtgt gtaggtttgc tcatcgtaac atatcctaaa atactggctc
      961 tttgtggtaa taacattact gtaaaaatta tctttgtcat catcttcatc accggcattg
     1021 cacgaagttt ttccggaccc gcttcatttg gtttatatgc acaaattgta cctaaagaag
     1081 tgtatcaaag tgccatcacc tggtcgagct cagcctggca attgggtgct gttttggggc
     1141 cggcttgtgg cggacttatt ctaggcatat tcggtattac tgccactttt gcttgtattg
     1201 ttttcttctt gataatcgct gttatgttga ttttgttaat tgataaaaag cctgttttat
     1261 ttgttcccca aaatgaatct gtttatcaaa atgctattca aggcttgaaa tttgtgtttg
     1321 aaaaaaaaga aatattagcc tgtatcagtt tggatttatt tgccgtattg tttggcggag
     1381 ccgttgcctt attgccggtt tatgccaaag atattttaca tgtaggggca caaggtttgg
     1441 gttggctgag ggcagcgcag gcgatgggag caatagttgt gttgctcttg ctggcttatc
     1501 acccgattaa aaaacaagcc ggaataaaat tattgcttgc cgtgtttggg tttggcgtgt
     1561 ttattatttt attcggcatc agtcgcaatt tttatttttc tatgttttgc ctgataatga
     1621 gcggtgcatt agatggtatc agtgttgccg tgcggcatac gattattcag cttgctacgc
     1681 ccgatgaaat gcgcggacgg gtttcagcag tcaattctat gtttatagga tcttcaaatg
     1741 aaattggcga atttgaaagc ggcctgactg cacactggat gggaacagtg ccggccgtaa
     1801 tctttggcgg ttgtatgact tgcctgattg tagccggaac ttatatcttc tctccgagat
     1861 taagaaaatt agaattgaaa taaaattttt gttaaacaga ttaaattaat ctcaaataca
     1921 atatatttac aatatcgctt cacccatgtc agatttacta tttacctacg gcaccttacg
     1981 gcttaatcaa agtcatccta tggctaatta cttagccgaa aatgccacct tagttggatt
     2041 ggcacttttg cccaaagcac gattatataa agtggattgg tatccggctt taacttttac
     2101 agataatgaa aacgatatgg tcgtgggaga tgtttttagc ttgaataatc tggatgcttt
     2161 agagaaatta gacgattacg aaggaatagg aaatggcgaa atcccgtatg aatacagacg
     2221 tgaaaaagta gaaattattt tggaaggaaa taaaacggct tgctgggtgt atttttataa
     2281 tcatccgctt ccggaaaatg cagagtggat agcatccgga gattttttaa atcc
//
LOCUS       DAOQII010000320         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_34802_length_2319_cov_5.013693, whole genome shotgun sequence.
ACCESSION   DAOQII010000320 DAOQII010000000
VERSION     DAOQII010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34802_length_2319_cov_5.013693"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLF48_11495"
     CDS             <1..406
                     /locus_tag="PLF48_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF49998.1"
                     /translation="EKVILIASAKTQKEIPFYYRFAGQLGLHKLLPTQLLQSSNFITN
                     WFFGTSSTFDKELLKQILIDTDPTFLKWAIDKVTRWTNQVQTKNIFHIHGTSDKILPL
                     SFINCNSIIKNGGHLMTLNKADELNQILRQQL"
     gene            complement(538..1272)
                     /locus_tag="PLF48_11500"
     CDS             complement(538..1272)
                     /locus_tag="PLF48_11500"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF49999.1"
                     /translation="MAKIDVNTTQNVTIQYDTAGLLWRFIAWLIDSAVYYIFIILMVF
                     FVFPFILDMSESFLYLFYFLVVVFYYFYRLLLEVFTNGQSIGKKLVSIKVLKLNGSSL
                     ESKDYFLRWAYRAVDGLLSGYTVGVLSILMSEKEQRLGDMIANTTVIKLKPDRVVTLE
                     DLQNLPDKAEYEPKYPQVTMFTDADMLALKSLLSRAQQYNNDTYYDLMAETVRNVKAK
                     MGIENVRTQDAAFLKEIISEYVILTR"
     gene            1356..>2319
                     /locus_tag="PLF48_11505"
     CDS             1356..>2319
                     /locus_tag="PLF48_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNF50000.1"
                     /translation="MKETRFIEQNLKKWKEFESLHQSNHKDSNLYSKLFIQITDDLSY
                     SRTFYGNRSVRVYLNNLAQDIFQRVFSRNRKKQNAFKDYITEELPSILFYHRKELLIA
                     FIILVVSILIGLFTYKHDGSFARKMLGDGYLEMTARNIAKKDPMAVYKDMNQVEMFVM
                     IVLNNLKVDLFTFFSGIFMGIGSILVLFQNGMMLAAFQYYFVKRGLFWDSFLAVWLHG
                     TLEISAMVICGAAGIVLGKGLLFPGTYSRMQSFLSSAQRASKIFFAVLPITFVAGIIE
                     SFLTRYTQMPDALRLGLILASLFFILGYFVYYPWLKYKNGTLRIP"
BASE COUNT          733 a          460 c          402 g          724 t
ORIGIN      
        1 cgaaaaggta attttaattg cttctgccaa gacacaaaag gaaattccat tttactaccg
       61 tttcgctgga caacttggac ttcacaaact tttgccgaca caacttttac aaagttcaaa
      121 ctttattaca aactggtttt tcgggacaag ctcaacattt gacaaagagt tattaaaaca
      181 aattcttatc gacaccgacc cgactttttt gaagtgggct attgacaaag taacacgttg
      241 gacaaatcag gtgcagacca aaaacatttt tcatattcac ggaacaagtg acaagattct
      301 acctttgagc tttataaatt gtaactcgat aattaaaaac ggtggacact taatgacact
      361 caataaagca gacgaactaa accaaatatt gcgacaacaa ttatgacaga caaacgaaaa
      421 actggacata acacgggtag ccgttcctcc cagggcggac aggtgcacaa cttcgttttc
      481 ccaaataagt aagcccgcct cattttgtgt ggatttaatg tagaaaagct cctgaactta
      541 tcgggtcaaa atcacatact cagaaataat ttcctttaaa aatgctgcat cttgagtacg
      601 cacattttca attcccattt ttgctttcac atttctaaca gtctcagcca ttaaatcata
      661 ataagtatca ttattgtatt gctgtgctct gctgagcaat gatttgagtg caagcatatc
      721 cgcatccgta aacatcgtaa cttgcggata tttgggttca tattctgcct tatcgggcag
      781 attctgcaaa tcttccaagg ttacaactcg atcgggcttt agttttatca ctgtagtatt
      841 ggctatcata tcgcccagtc tttgttcttt ttcactcatc aatatggaca atacaccaac
      901 ggtgtaaccg gataataaac catctacagc ccgatatgcc caacgcaaaa aataatcttt
      961 actttccagc gaactgccat ttagttttaa cacctttatg gaaaccaatt ttttaccaat
     1021 actttgtcca ttggtaaata cctcaaggag tagtctgtaa aaatagtaaa acactactac
     1081 cagaaaatag aaaagataca aaaaggactc cgacatatcc agaatgaacg gaaatacaaa
     1141 aaaaaccatc aatatgataa aaatataata gaccgcacta tctatcagcc aggcaataaa
     1201 acgccacagt aacccggcag tatcgtactg aatagttaca ttttgcgttg tatttacatc
     1261 tatctttgcc atgcgatgcc gaatttatat aatttttcag atttatttaa ccaaatgcat
     1321 tctcgatgtg caaaaatata ttattttagt cggacatgaa agaaacgcgc tttattgagc
     1381 aaaatctgaa aaaatggaaa gagtttgagt cattgcatca aagcaatcac aaagactcca
     1441 acctttacag taaactgttc attcagatta ctgatgattt atcatattca cgcacttttt
     1501 acggcaaccg ttccgttagg gtatatctca ataatttggc acaggatatt tttcagcgtg
     1561 tcttcagcag aaaccgtaaa aaacaaaatg ctttcaaaga ttatatcacg gaagaactgc
     1621 cttccatatt attttatcac cgcaaagaat tactcattgc ttttatcatt cttgttgtat
     1681 cgatactcat tggtttgttt acctataaac atgatggaag ttttgcaaga aaaatgttgg
     1741 gagacggcta tttggaaatg accgcccgaa atattgccaa gaaagatcca atggcggtgt
     1801 ataaagacat gaatcaagta gaaatgtttg tgatgattgt actcaacaat ttgaaagtcg
     1861 atttatttac tttcttttcc ggcatattta tgggtatcgg ctctattttg gttttatttc
     1921 aaaatggtat gatgctggcg gcatttcaat attattttgt taaacgcgga ttattctggg
     1981 attcatttct tgctgtatgg ctgcatggca cactggaaat ctcagcgatg gttatatgtg
     2041 gtgctgcagg tatcgtattg ggaaaaggtc tgttatttcc gggtacatac agtcgaatgc
     2101 aatccttctt atcatctgca caacgagcat ccaagatctt ttttgctgta ctgcccatca
     2161 ccttcgttgc cggtatcatc gagtctttct tgactcgata tacacaaatg ccggatgcat
     2221 tgcgtttggg attgatactg gcatcgctgt tttttatctt gggttatttt gtttattatc
     2281 cctggttaaa atacaaaaac ggcactttgc gcataccgg
//
LOCUS       DAOQII010000321         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_35285_length_2302_cov_5.000890, whole genome shotgun sequence.
ACCESSION   DAOQII010000321 DAOQII010000000
VERSION     DAOQII010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35285_length_2302_cov_5.000890"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PLF48_11510"
     CDS             complement(<1..1046)
                     /locus_tag="PLF48_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF50001.1"
                     /translation="MRQPHFLVYFLPYLIFLLLDWYVFQALKTAFPSLKNSTGRLFLV
                     GYILFSLLPYLLVTVYLMVGFSGWTGISKTILIGIAQLIVLSKLFVLPFLLFDDITRF
                     FKWIFSLFSQTPKTSEAGITRSQFLSRAGLLAGGTMFGVFLYGIAIGAYNYRKRKITL
                     AIPDLPDEWKDLKIVQISDLHLGSFSSTHPTEKIVELINAEQPDFIFFTGDLVNYAGT
                     EVDSHVEVLKKLKAKEAIYSILGNHDYGMYNDWNSKEEQNENFRHLLAQQKALGWDLL
                     LDEHRILERNNKTLAIIGVQYIGHSLRFGQFGNLDKAYAGAEHADIQLLLSHDPSHWD
                     KDVSVGEKYKKIHAT"
     gene            1109..>2302
                     /locus_tag="PLF48_11515"
     CDS             1109..>2302
                     /locus_tag="PLF48_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNF50002.1"
                     /translation="MLFNIKHKKLLFAFILINGLILSLQSFVGTQAPKEAVRQTALQD
                     INVFKSTLDTLQIALQNKADIKSLHLQFKQTRLAFKKFEWLLEYFDSGNYRFFNGTNA
                     VEFEYGYQPNMQPEGLQNIETLLYADSLQSETLILLVNKLKYKTLTFYSYFTDAELKD
                     DYIFSAMRYHLIRIEALNLASFDSPDLQNNTQELAVALSSLLDVVSAYENKQNIQQVK
                     LLRSRIQLAVKYLQTKNFNTLDRLTFLKKYFYPLVQAYFSFLKAQQFQFERDYAGMIK
                     PVNLQKENIYDADFLIPKYFAQDKYYTDDTLLVQLGKKLFFDQQLSADGKLSCATCHQ
                     PEHAFADKEITSITNQKGIFQKRNTPSLLNTALQPAYMYDMRANSLESQIHLVVSNPQ
                     EFNHSF"
BASE COUNT          765 a          451 c          385 g          701 t
ORIGIN      
        1 gtagcatgta tcttcttata tttttcaccc acactcacat ctttatccca atgtgaaggg
       61 tcgtgagaca gtaacaactg gatgtctgca tgttcagctc cggcataagc tttatctaaa
      121 ttgccaaact gaccaaagcg cagactgtga ccaatatact gcaccccgat aatcgccagc
      181 gtcttgttgt tgcgttccaa aatgcggtgt tcatccaaca gcaaatccca gcctaatgct
      241 ttttgttgcg ccagcaaatg cctgaaattt tcgttttgct cttcttttga gttccagtca
      301 ttatacatac cataatcatg gttgcctaaa atggaataaa tggcttcttt tgcttttaat
      361 tttttcaata cttcaacatg actatctact tctgtacccg cataattgac taaatcgcca
      421 gtaaaaaaaa tgaaatccgg ttgttcggca ttgatgagtt cgacaatttt ttcagttgga
      481 tgtgttgaag aaaatgagcc taaatgtaaa tcagaaatct gcacaatttt caagtctttc
      541 cattcatcag gcaaatcagg aatagctaaa gttatttttc tttttctgta attatacgca
      601 cctatggcaa taccatataa aaatacaccg aacatagtgc cacctgccag caaaccggct
      661 ctgctcaaaa attgcgaacg ggtaatacct gcttcacttg tttttggcgt ttgactaaac
      721 aagctgaata tccatttgaa aaagcgagta atatcatcaa aaagtaaaaa tgggagtaca
      781 aataattttg aaagtacaat gagttgtgct atgccgatta atattgtttt ggagatgcct
      841 gtccagcccg aaaaaccaac cattaaatac accgtaacta agagataagg caacagcgaa
      901 aataggatat aacccactaa aaacagcctg cctgttgaat tttttaaaga tggaaatgct
      961 gtttttaatg cttgaaagac ataccagtct aacaataaga aaatgagata cggtaggaag
     1021 taaactaaga aatgtggctg ccgcataaat ctttttattt tttgaaacca caaaaataca
     1081 gactgaacac agtaaatttg ctattaacat gttatttaac ataaagcata aaaaattact
     1141 gtttgcgttt attttaataa atgggctgat attgagtctg caatcttttg ttggcacaca
     1201 agcaccaaaa gaagcagtac gtcagacagc attacaggat attaatgttt tcaaatctac
     1261 attagacacc ttacaaattg cgctacaaaa caaagctgat atcaaaagtc tgcatttgca
     1321 atttaaacaa actcgattag cgtttaaaaa atttgagtgg ctgctggaat attttgattc
     1381 gggaaattat cgttttttca acggtaccaa tgccgtagag tttgaatatg gctatcagcc
     1441 gaatatgcaa cctgagggtt tacaaaatat cgaaacttta ttgtatgctg attcgttgca
     1501 atcagaaacg ctaatacttt tggtcaataa gctcaaatac aaaacgctga ctttttattc
     1561 gtatttcaca gatgcagaac ttaaggatga ctatattttt tctgccatgc gctatcatct
     1621 catccggata gaggcattga atttagcctc atttgactcc cctgatttgc aaaataatac
     1681 acaagaactg gcagtagctt tgtcaagcct tcttgatgtt gtttcggctt atgaaaacaa
     1741 acaaaacatt caacaagtaa aattactccg aagtcggatt cagcttgcgg tgaaatatct
     1801 acaaactaaa aatttcaaca ctttagaccg gcttactttt ctaaaaaaat atttttatcc
     1861 cctagtgcaa gcgtatttta gctttttaaa agcacagcaa tttcaattcg aacgagatta
     1921 tgcaggaatg attaaacccg taaatttaca aaaggaaaac atctatgatg cagacttttt
     1981 gattccaaaa tattttgctc aagacaaata ttatactgac gataccttat tagtacaatt
     2041 gggtaagaaa ttgttttttg accaacaact gtctgccgat ggaaaactta gttgtgccac
     2101 ttgccatcag ccggaacatg cctttgccga caaagaaatt acatcaatca ccaatcaaaa
     2161 gggcattttt caaaagcgca ataccccttc cctgctcaat acggctttgc agccggccta
     2221 tatgtacgat atgcgtgcca acagtctgga atcacaaatt catttagtcg tatcaaatcc
     2281 gcaggaattt aatcattcat tt
//
LOCUS       DAOQII010000322         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_35641_length_2290_cov_3.520358, whole genome shotgun sequence.
ACCESSION   DAOQII010000322 DAOQII010000000
VERSION     DAOQII010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35641_length_2290_cov_3.520358"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLF48_11520"
     CDS             <1..470
                     /locus_tag="PLF48_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF50003.1"
                     /translation="ISMPVIDGYELTKEIKKINPEIQVIALSMSGDITSINKMLDAGI
                     SGYVLKNAGNEELVDAIHKVAEGKMHFSDEVLAEMSRSANTKQTKQDKINLSDREIEI
                     LKLISLEHNNAEIADLLFISERTVETHRKNMMRKTNSKTMIGLLRTAMEHKLI"
     gene            527..1315
                     /locus_tag="PLF48_11525"
     CDS             527..1315
                     /locus_tag="PLF48_11525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF50004.1"
                     /translation="MSNLYYFGEYISYKISSLTKYKIHSPFIFKLVTEVFEKEVKYDD
                     YERIEQIRDGLLHDKRIIEVNDLGAGSKTFTSKMRAVSKIARRSVKKEKYAHLLYMMI
                     RHYKANTILELGTSLGITTAYLASAHRNVHVYTIEGCKNIASIAEEGWKKLLLRNIDL
                     TVGNFDDVLKDVCIKAGKLDLVFIDGNHREIPTINYFKIILPYCHNDTVIVLDDIHWS
                     EEMEEAWEYIKNHEKVRLTVDIFQMGIVFLREEFRAKQHMQISY"
     gene            1336..1830
                     /locus_tag="PLF48_11530"
     CDS             1336..1830
                     /locus_tag="PLF48_11530"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNF50005.1"
                     /translation="MTIRLFITGGTFDKEYNELNGKLFFEDTHVPEMLDLGRCKLDVD
                     IRTLMMIDSLEMTEEDRDIIALNCIKCEEDKIVITHGTDTMVDTAKILAEKIKDKTIV
                     LTGAMIPYKFGSSDGLFNLGAALAFVQTLPKGIYIAMNGKYFNWDNCRKNKELGLFEV
                     IHSE"
     gene            1854..2246
                     /locus_tag="PLF48_11535"
     CDS             1854..2246
                     /locus_tag="PLF48_11535"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNF50006.1"
                     /translation="MKLLSITILFLLSSFLLFADIKTLTEQQIKDMVSHKWRLTTIEV
                     KGKTIEVTDDKPMIALKFNNNGTMDEYAGDKHFTGNWTYTHTDYTIETKDKDGKEKHT
                     ILDISDKLLKMTTKFMGMKVVYVMKRVD"
BASE COUNT          863 a          363 c          436 g          628 t
ORIGIN      
        1 atatcagcat gccggtaata gacggctatg aactcacgaa agaaattaaa aaaattaatc
       61 ctgagattca ggtcattgca ctatctatgt cgggtgatat tacgagtatt aataaaatgc
      121 tggatgccgg catttcgggt tatgtcttaa aaaatgctgg taacgaagag ctggtagacg
      181 caatacacaa agtagccgaa ggcaagatgc attttagcga tgaagtgctg gctgagatgt
      241 cgcgctctgc aaatacgaag caaacaaaac aagataaaat caatctgtcc gacagagaaa
      301 ttgagattct caagctcatt tcgcttgaac ataacaatgc cgaaattgca gatttattgt
      361 ttatttcaga acgcacagta gaaacacacc gcaaaaatat gatgcgaaaa actaactcca
      421 aaaccatgat tggattattg agaacagcca tggaacataa gttgatttaa ttacaaataa
      481 tatcgtattt ttatccaaac tgcaaagtat ctgctaaatc tgacttatga gtaatcttta
      541 ttattttggt gaatacatat cctataaaat ttcatccctg acgaaataca aaattcattc
      601 gccttttatt ttcaagttag tcacagaagt atttgaaaaa gaagtaaaat atgatgatta
      661 cgaaagaata gaacaaatca gagatggtct tttgcacgac aaacgcatta tagaagtaaa
      721 tgatttaggc gcaggatcga aaacctttac atcaaaaatg cgggctgttt caaaaattgc
      781 ccgacgaagt gtaaaaaaag aaaaatacgc tcatctgctt tatatgatga tacgtcatta
      841 caaagccaat actatcttag aattaggcac ttcgcttggt attactacgg catatttggc
      901 ttcggcacac cgaaatgtac atgtatatac aatagaagga tgtaaaaaca tagcaagcat
      961 tgcagaagaa ggctggaaaa aactgctgtt gcgaaatata gatcttaccg taggaaattt
     1021 tgatgatgta ctgaaagatg tttgcataaa agccggaaaa ttagatttag tttttattga
     1081 cggcaatcat cgtgaaatac cgactataaa ttattttaaa atcatattgc cttattgtca
     1141 caatgataca gttattgttt tagatgatat ccattggtcg gaagaaatgg aagaagcctg
     1201 ggagtatata aaaaaccacg aaaaagtaag acttactgta gatatttttc aaatgggcat
     1261 cgtgtttttg cgcgaagaat tcagggcaaa acaacacatg cagattagct actgataata
     1321 aaataaatcc ggaaaatgac aatacgatta tttattacgg gcggcacatt tgacaaagag
     1381 tataacgaac tcaacggcaa attgtttttt gaagatacac atgttcccga aatgctggat
     1441 ttaggcagat gcaaattaga cgtagatatc cgcacactga tgatgataga cagtttagaa
     1501 atgaccgaag aagaccgaga cattattgca ttgaattgca taaaatgtga ggaagataaa
     1561 atcgtgatta cacacggaac ggataccatg gtagatactg caaagatttt agccgaaaaa
     1621 attaaagata aaacaatcgt attaaccggg gctatgatac cgtataaatt tggcagttct
     1681 gacggattgt ttaacctggg tgctgccctt gcctttgtac aaacgttacc caagggtatt
     1741 tatattgcga tgaacggcaa atattttaat tgggataatt gcagaaaaaa caaagaactg
     1801 ggtttatttg aagttattca ttctgaataa aatcacaatt gataaatatg caaatgaaat
     1861 tactaagtat caccatttta ttcctgttga gcagcttctt actatttgct gacattaaga
     1921 ctttaaccga acagcaaatc aaagatatgg taagccacaa atggcgacta acaaccatag
     1981 aagtaaaagg taaaaccata gaagtaacag atgacaaacc catgattgct ttgaagttta
     2041 acaacaatgg cacgatggat gagtatgccg gggataagca ttttacaggc aactggacat
     2101 acacgcatac cgactatacc attgaaacga aagataaaga tggaaaagaa aaacatacca
     2161 ttttagatat ttcagacaag ctactaaaaa tgacaaccaa atttatgggg atgaaagtgg
     2221 tttatgttat gaaacgagta gattaatcta aaaataaatc acgcatcatt ttttaaagtg
     2281 aatattcagt
//
LOCUS       DAOQII010000323         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_36737_length_2253_cov_3.870792, whole genome shotgun sequence.
ACCESSION   DAOQII010000323 DAOQII010000000
VERSION     DAOQII010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36737_length_2253_cov_3.870792"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1237)
                     /locus_tag="PLF48_11540"
     CDS             complement(233..1237)
                     /locus_tag="PLF48_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNF50007.1"
                     /translation="MKVESRKYKVLIHDIGSILEQSRLNAVKLINYELVKAYWEIGRH
                     IVNYEQHGNERAEYGTSMLSGLSKDLTEKYGKGFGRRNLSDMRRFYLIFDDWNHVSQK
                     LYWSHYVLLLSVTDDEKRKFYFDKCNAENWSVRTLENQLDSSLYERWMLQHKKKGISL
                     LPKNKQELPDIADVIKDPYVLDFLKIPKNHKLTEKSFEQKIINNLQSFILELGKGFAF
                     IGRQYKISLGSSHFYVDLVFYHRILKCFVLIDLKIRNVKHNDIGQMNLYLNYFKAEEN
                     VEGDNDPIGIILSAEKDEILVQYAMGGISNKIFVSKYKLYLPDKKILQRKVSDLLHEK
                     "
     gene            complement(1234..1833)
                     /gene="cysC"
                     /locus_tag="PLF48_11545"
     CDS             complement(1234..1833)
                     /gene="cysC"
                     /locus_tag="PLF48_11545"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNF50008.1"
                     /translation="MNEHIHPVFDKLLQREDKERLLHQKSVCIWMTGLSGSGKSTIAQ
                     GLERKLQQEGILCFVLDGDNVRTGINKNLGFSEEDRTENIRRIAEVNKLFLNCGVITI
                     NCFVSPTKEIRENAKAIIGEHDFYEVYVNASFDECAKRDVKGLYAKALKGEIKNFTGL
                     DAPFEAPANPAVEINTAEQSIEESVEMVFNYFMNKIKQV"
     gene            complement(1902..>2253)
                     /locus_tag="PLF48_11550"
     CDS             complement(1902..>2253)
                     /locus_tag="PLF48_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNF50009.1"
                     /translation="FVGVDSGQNHLIRPMFYDAYHHITNISNPEGIHRIYTVVGYICE
                     TDTFGWDRKLNEVREGDILCLHNAGAYGFSMSSNYNSRYRPAEVLIKDGKAQLIRRRE
                     NLEDLLATQVLIED"
BASE COUNT          678 a          471 c          324 g          780 t
ORIGIN      
        1 cctcacggtg tatccattca tcttcttcaa tttcttttat ttttattttt tcatcttgat
       61 tacttaccga agccgcatga ccctgataag caaacgccgc agctctttca gcagaataag
      121 ccagttgcag taaattctct aactcaggtt gcaattgcat ttcactttag tattatcgca
      181 tatcatattc tttatacatc atatcgtatt tggcagacac gtctgccaaa tttcattttt
      241 catgtagcaa atcagatact tttctttgta agattttttt atccggcagg tacaatttat
      301 atttagaaac aaaaatttta ttggatattc cgcccatggc atactgcact aatatttcat
      361 ctttctctgc cgataaaatt attccaatcg gatcattatc tccttctacg ttttcttctg
      421 ctttaaaata attcaggtac aagttcattt gcccgatatc gttgtgtttt acatttctta
      481 ttttcaaatc tatcaagaca aaacatttta agatgcgatg ataaaaaact aaatccacgt
      541 aaaaatgaga actgcctaaa gaaattttat attgtcttcc gatgaaagca aaaccttttc
      601 ctaattcaag aataaaagat tgtaaattat tgataatttt ttgttcaaat gatttttcgg
      661 taagcttatg atttttcgga attttcaaaa aatccaagac ataagggtct tttattacat
      721 cagcaatatc cggcaactct tgtttgtttt tgggtagtag tgaaattcct tttttcttat
      781 gctgaagcat ccatcgttca tacaaagaac tatccagttg attttctaaa gtacgcacgc
      841 tccagttttc cgcattacat ttatcaaaat aaaacttgcg tttttcgtca tccgtaacgg
      901 acaataaaag tacataatgg ctccagtata atttttggga aacatgattc caatcatcaa
      961 aaatcaaata aaatctacgc atatctgaaa gattgcgtct gccaaatcct tttccgtatt
     1021 tttctgtcaa atcttttgaa agacctgata acattgatgt tccgtactca gcacgctcat
     1081 tgccgtgttg ctcataattc acgatatgtc tgccaatttc ccagtaagct tttactaatt
     1141 cgtaattaat taattttaca gcattcaatc gagattgctc taaaatcgat ccgatatcat
     1201 gaattaaaac tttatatttc cgactttcga ctttcaaact tgttttattt tattcataaa
     1261 ataattgaat accatttcca cactctcctc aatactttgt tcggctgtat taatttcaac
     1321 agcaggattt gcgggagctt caaacggcgc atccaaaccc gtgaaattct tgatttctcc
     1381 tttcaacgct ttagcataca aacctttcac gtcgcgtttg gcgcattcat caaaactcgc
     1441 atttacatat acttcataaa aatcatgctc accaattatc gcttttgcat tttctcgaat
     1501 ttctttagta ggagacacga aacaatttat cgttataacc ccacaattca aaaataactt
     1561 attgacttcg gcaatacgcc gtatattttc cgttctgtct tcttccgaaa aaccaagatt
     1621 tttattaatc cccgtgcgca cattatcgcc atccagcaca aagcataaaa taccttcttg
     1681 ttgaagtttt cgttccaaac cttgagcaat ggtagatttg cccgaacccg ataagcccgt
     1741 catccaaata cagacagatt tttgatgcaa caaacgctct ttatcttcgc gttgaagcaa
     1801 tttatcaaaa acaggatgga tatgttcgtt cattttcagc ccgtaaaaat aaataaaatt
     1861 atttacatcc accaatagta tatcgaaaga gtagagtatt cttaatcttc aattaaaacc
     1921 tgtgtagcta ataaatcttc taagttttct ctgcggcgta taagttgggc tttgccatct
     1981 ttgattaata cttctgcagg tctgtatcga gaattgtagt ttgaagacat cgagaagcca
     2041 tacgcccctg cattgtgcag acaaaggata tcgccttctc gcacttcgtt gagtttcctg
     2101 tcccaaccaa atgtatccgt ttcacaaata taaccgacca cggtataaat tctgtgtatg
     2161 ccttccggat tggaaatatt agtgatgtga tggtatgcat cataaaacat gggacgaatg
     2221 agatggttct gtccactatc cacgcccacg aat
//
LOCUS       DAOQII010000324         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_37729_length_2221_cov_4.628347, whole genome shotgun sequence.
ACCESSION   DAOQII010000324 DAOQII010000000
VERSION     DAOQII010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37729_length_2221_cov_4.628347"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1918
                     /gene="aceB"
                     /locus_tag="PLF48_11555"
     CDS             323..1918
                     /gene="aceB"
                     /locus_tag="PLF48_11555"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HNF50010.1"
                     /translation="MSQYPHLEGVEIRGEFRPEWAEIITPEALAFFAKLQRKHNARRK
                     ELLQKRVERQKQLDAGVLPDFLPETKHIRESAWTVAPCPADMQDRRVEITGPVDRKMI
                     INALNSGAKVFMADFEDSNAPTWENNIQGQVNLKDANLKTISFVNEAGKEYKLNEQHA
                     VLFVRPRGWHLLEKHVWVDGEEASGSIWDFALYFFHNAKTRLAQGSGIYFYLPKLESH
                     LEARLWNDVFVDAQNELGVPQGSVKATVLIETIVATFELNEILWELKEHSAGLNCGRW
                     DYIFSFIKRTVKNKQFLVPDRMQVTMTVHFLKSYSELVIQTCHKREAFAMGGMAAQIP
                     IKDNPEANEIALAKVRADKEREAKAGHDGTWVAHPGLVQIAMDEFNKYMPTPNQIFKK
                     REDVHVTAADLLEVPKGTITLGGLKNNIDVGIQYLEAWLRGNGCVPIHNLMEDAATAE
                     ISRTQVWQWVHHEDAKLDDGTKVTALLVQNLIDEQKAAYKTALGDKYANSKFDLAIDL
                     FTKMSLEDKLEEFLTLEAYNFID"
     gene            1930..>2221
                     /locus_tag="PLF48_11560"
     CDS             1930..>2221
                     /locus_tag="PLF48_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF50011.1"
                     /translation="MLHPIRNIIQGYKNKQRITHSIANRKDIINTWIKNGKPTPTPHE
                     VKQVAIEEYKNLYQCKCLVETGTYLGDMMEIQKNNFEQLYSIELSEMLYNRAI"
BASE COUNT          764 a          395 c          436 g          626 t
ORIGIN      
        1 agttgatttt ttatatagaa ccacctcctt ttaattccac ctccttgtta agttggagga
       61 aaaaatgaga aatcaactat ttttttcacc ttaattttct taactcgaac tcaggttatt
      121 aggacgtgca taatacatca tctcatattt gtttcaaaat cttgtgccat actattttct
      181 tcctactttt attaatttga taaaaaaaat tccaatgcat gcattgaaaa attaaacctt
      241 atcataatct ttaggtaaca tcttttttcg tgctaaaatc tgcattatag ctaatttcgt
      301 aaaaaagaaa ttttactcga atatgtcgca atatcctcat ttggaaggtg tagaaataag
      361 aggcgaattc agaccggaat gggctgaaat cattacaccg gaagcgcttg cttttttcgc
      421 taaacttcag cgcaaacaca atgcccgcag aaaagaatta ttacaaaaac gtgtagaacg
      481 tcaaaaacaa ctggatgccg gtgtattgcc tgacttttta ccggaaacaa aacatattcg
      541 tgaaagcgca tggacggttg ccccctgccc tgccgatatg caagaccgaa gagtggaaat
      601 aaccggtcct gttgaccgca aaatgattat caatgcgctc aattccggtg ctaaggtttt
      661 tatggcagat tttgaagact ccaatgcacc aacatgggaa aacaatattc aaggtcaggt
      721 caacttgaaa gatgcaaatc ttaaaacaat ttcttttgta aacgaagccg gaaaagaata
      781 taaactcaat gaacagcatg ccgtattatt tgttcgtccg cgcggctggc atttattgga
      841 aaaacatgtt tgggtagatg gcgaagaagc cagtggttct atctgggatt ttgcccttta
      901 tttctttcac aatgctaaaa ccagactggc acaaggaagt ggtatttatt tctacttacc
      961 caaattagaa tcgcatttag aagctcgttt gtggaacgat gtttttgtag atgcacaaaa
     1021 tgaattaggc gttccacaag gttctgttaa agcaacggta ctgatagaaa ccattgttgc
     1081 tacttttgaa ttgaatgaaa tattgtggga actgaaagaa cacagtgccg gattaaattg
     1141 cggtcgttgg gattatattt tctcctttat aaaacgcacc gtaaaaaaca aacaattctt
     1201 agtgcctgac agaatgcagg taacgatgac tgttcacttt ctgaaatctt attctgaatt
     1261 ggtgatacaa acttgccata agcgcgaagc ttttgctatg ggcggtatgg ctgcacaaat
     1321 tccgataaaa gacaatccgg aagcgaacga aatagcactg gcaaaagtgc gtgccgacaa
     1381 agaacgcgaa gccaaagccg gacatgacgg cacttgggta gctcatccgg gattggtaca
     1441 aatagccatg gatgaattca acaaatacat gccaacaccc aatcagatct ttaaaaaaag
     1501 agaagatgta catgttactg cagccgattt gttggaagta ccaaaaggca caattacatt
     1561 aggcggtttg aaaaacaata tagatgtagg tattcaatat cttgaagcgt ggttgagggg
     1621 caacggttgt gtgcctatac acaacttaat ggaagatgcg gctactgcag aaatatccag
     1681 aacacaggtt tggcaatggg tacatcatga agatgcaaaa ttagatgatg gcacgaaggt
     1741 aacggcactc ttagttcaaa atctgattga tgaacaaaaa gcagcctata aaacagcctt
     1801 gggagataaa tatgctaact caaaatttga tttagccatt gatttattca cgaagatgtc
     1861 tttagaagat aaactggaag aatttttaac tttggaagcg tataatttta tagattaatt
     1921 tttgaaaaca tgcttcatcc aattagaaat ataattcaag gctacaaaaa caagcaacgt
     1981 ataacgcaca gcattgcgaa caggaaagat attataaaca cctggataaa aaatggcaaa
     2041 cccactccta ctcctcatga agtaaaacaa gtggctattg aagaatataa aaatctgtat
     2101 caatgtaagt gcttagtaga aacgggtact tatttgggtg atatgatgga aatacaaaaa
     2161 aataattttg agcaattgta ctccattgaa ttaagcgaga tgttatacaa ccgtgctata
     2221 a
//
LOCUS       DAOQII010000325         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_38731_length_2190_cov_5.399532, whole genome shotgun sequence.
ACCESSION   DAOQII010000325 DAOQII010000000
VERSION     DAOQII010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38731_length_2190_cov_5.399532"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..802)
                     /locus_tag="PLF48_11565"
     CDS             complement(116..802)
                     /locus_tag="PLF48_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF50012.1"
                     /translation="MRIFFFLLILMLSGFFKSVFAQDDIVLDLDSIKNIKTTRYSTIN
                     EVGANINVSGMMIQKLPGTQKKTTLQTDKPGMLFRTVHGALINPNLFIGAGTGFDFRP
                     NGTNGTSYYFFTFPFFAESREYFLNGSFRMFAAQRLGASLYVDSYYLKYYKKTSSKGA
                     FMEILFGGRYVKPGKNFALHFSAGYRFQHLQRKVDVQSVSGGTNIQIYSNTPEITLKH
                     YIPITIGITY"
     gene            1055..1210
                     /gene="rpmH"
                     /locus_tag="PLF48_11570"
     CDS             1055..1210
                     /gene="rpmH"
                     /locus_tag="PLF48_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNF50013.1"
                     /translation="MKRTYQPSVRKKKNKHGFRERMKTKGGRAVLSARRAKGRKKLSV
                     SDEKHTK"
     gene            1231..1608
                     /gene="rnpA"
                     /locus_tag="PLF48_11575"
     CDS             1231..1608
                     /gene="rnpA"
                     /locus_tag="PLF48_11575"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNF50014.1"
                     /translation="MRFTFTATERLKSKKEIDLLFGQKLFVQNPSFRIYYLTEKTATQ
                     NLLKFAFSVSKKSFPHATDRNRIKRQMREAVRKHKQEWKEKLADKGLSCNFLIVSRFQ
                     QKPEFQTVEKGILSLFNKLTDAV"
     gene            complement(1620..>2190)
                     /locus_tag="PLF48_11580"
     CDS             complement(1620..>2190)
                     /locus_tag="PLF48_11580"
                     /inference="COORDINATES: protein motif:HMM:NF016021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF50015.1"
                     /translation="KVVIRPDSGDPADIICGNPSGKTIEERKGVVQLLWEVFGGKENA
                     KGYKELDPHIGAIYGDSINMERAATICERLKQNGFASTNVVLGIGSYTYQYNTRDTFG
                     FAMKATYGEVYGEGRSIFKSPVTDDGTKNSAKGLLKIVKHKGQYQLIDNVSWTEEKQG
                     ELIEVFRDGQLLVNHSLKDIRARVNNVDR"
BASE COUNT          667 a          438 c          411 g          674 t
ORIGIN      
        1 aattcattcg atataatcgg gatgtctcct tgtgattcct cctccttgtt aatctggagt
       61 gaaatgcaaa aaatcaacta ttttttcacc ttgtttttct ttacactatc tcagattagt
      121 acgtgatgcc gatggtgatg ggtatgtaat gtttgagggt gatttcgggt gtgttggagt
      181 aaatctggat atttgtgcct ccgcttacgc tttgcacgtc tactttgcgc tgcaaatgct
      241 ggaagcgata tcctgcgctg aagtgcaggg caaagttttt gccgggtttt acatatcgtc
      301 cgccaaataa aatttccata aacgcccctt tggacgacgt tttcttgtag tatttcaaat
      361 agtaggaatc aacataaaga gatgcgccaa gacgttgcgc agcaaacatg cgaaaactgc
      421 cgttcaaaaa atactcccgg gattcggcaa aaaacgggaa tgtaaaaaaa tagtaactgg
      481 taccattagt accattaggc cggaaatcga atcctgtgcc tgccccgata aacaaattgg
      541 gattaatcaa tgctccgtgt accgttctga aaagcatgcc gggtttgtcg gtttggagtg
      601 ttgttttttt ttgtgttccg ggcagttttt gtatcatcat accggataca tttatgttcg
      661 ctcccacttc atttatggta gaataccggg tcgtttttat gttttttatg gaatctaaat
      721 ccaacacgat atcatcctgg gcaaaaaccg acttaaaaaa tcccgacagc attaatatca
      781 ataagaagaa gaaaatgcgc atgatgaatg aagtttatcc gtaaaattag taagaatcac
      841 ttggattctg tttgctttag gattaatttt aaaagcgtat aatgacatct tgattatatg
      901 ctgacggttt ttcgtgcatc atttggcata acaattagat aatcagaata attttagctc
      961 aatttctgag ttttaaacaa aaaaaacgta tcttcgcctc ccgatttgtg gaaaactgca
     1021 tgtcggttta ttaatctttt agaaatcatc cataatgaaa agaacatacc aaccgtcggt
     1081 aagaaagaag aaaaacaaac acggttttcg cgagcgtatg aaaacaaaag gtggtcgtgc
     1141 tgtattgagt gcccgcagag ctaaaggcag aaaaaaattg tctgtatctg acgaaaaaca
     1201 cactaaataa tagctgaaca tacttcaaac atacggttca ctttcaccgc tactgaacgc
     1261 ctgaaaagca aaaaagaaat tgatttgctc tttgggcaaa agttatttgt acaaaatcca
     1321 tcttttcgca tttattatct tacagaaaaa acagcaaccc aaaatttgct gaaatttgca
     1381 ttttctgttt ctaaaaaatc atttccacac gccacagacc gcaaccgcat caaaagacaa
     1441 atgcgcgaag cggtaagaaa gcataaacag gagtggaaag aaaaattagc cgacaaaggg
     1501 ctatcttgca attttctgat tgtgagcagg tttcaacaaa aacccgaatt ccaaacggta
     1561 gaaaagggca ttttgagctt gtttaataag cttactgatg ctgtgtaatt ttaaatatat
     1621 cagcggtcaa cgttatttac tctggctcga atatctttta atgaatgatt gactaaaagt
     1681 tgtccgtcac gaaatacttc tataagctct ccttgttttt cttcagtcca gcttacatta
     1741 tcaatcaatt gatactgacc cttatgcttt acaatcttca agagcccttt ggctgaattt
     1801 ttagtaccat catccgtaac agggctttta aaaatagacc gcccttcgcc atacacttca
     1861 ccatacgtgg ctttcatcgc aaaaccaaaa gtatcacgtg tgttatactg ataagtatat
     1921 gagccaattc ccaatacgac attggtagat gcaaacccat tttgttttaa gcgttcacaa
     1981 atagttgcgg ctcgttccat gttaatactg tcgccataaa tggcaccaat atgcgggtcg
     2041 agttccttat agccttttgc gttttcttta cctccgaata cctcccacaa aagttgtaca
     2101 actccttttc tttcttctat ggttttgccg gatggattgc cacaaataat gtctgccgga
     2161 tcgccactgt cgggtcttat taccactttg
//
LOCUS       DAOQII010000326         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_38949_length_2184_cov_4.560357, whole genome shotgun sequence.
ACCESSION   DAOQII010000326 DAOQII010000000
VERSION     DAOQII010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38949_length_2184_cov_4.560357"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..211)
                     /locus_tag="PLF48_11585"
                     /pseudo
     CDS             complement(1..211)
                     /locus_tag="PLF48_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            312..1544
                     /locus_tag="PLF48_11590"
     CDS             312..1544
                     /locus_tag="PLF48_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF50016.1"
                     /translation="MIEFNSIQLHNGLTLIHHEDTTTPFVVVNTLYKVGAKDEDEHRT
                     GFAHLFEHLMFGGTKNFPDFDKPLQESGGENNAFTNNDYTNYYDIVPAENIEIPLCLE
                     ADRMTQLNINTKSLSVQRKVVCEEFKEHYINQPYGDVWHLIREMVYEKHHYKWPTIGK
                     ELKHIEEATLDDVRSFYQKYYQPGNAILVIAGNINWDKTQELVQHYFGAISSTSVKHS
                     LITEPEQTAAKTKTVHEEVPFSALFINFKMEDRLNKAYYTADICSDLLSNGTASRLQK
                     KLVKEKKVFIEIDCYISGSVETGIFTVEGKVKEDIDMTYAEQLVWEELEKLKTGDVHE
                     NELTKCKNKMLTYMNFSDSSLLNRSISLAFYELLGDAQLINKEEEFYTNITSADVQAY
                     AKSLFVKEKSNTLYYLKK"
     gene            1569..>2184
                     /locus_tag="PLF48_11595"
     CDS             1569..>2184
                     /locus_tag="PLF48_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNF50017.1"
                     /translation="MNLPPIHDIQQIQPALPDTKVLANGIVLHGFNGLKNDILKLDLV
                     FNAGKWVEPQQMVAEFTARLFKSGTTQFDAHALSEKIEFLGSSIKAGVGYNTFTVSVS
                     CMNKHLEATLGLLLICLNEINFPENEITHEQQKAKSQLKLALEKNDYIADITFRKLLF
                     GENHPYGYASTDATIDAIAHKSLLDYYNTYVIPSLCTLYIAGKYA"
BASE COUNT          732 a          406 c          416 g          630 t
ORIGIN      
        1 attctctgat aatgttttct attaacactc gactggaaga tatcttttga ttttgtattt
       61 tgtcttcctt tgtcaaagga tttctcttgc tgcgtttttt aggaagctca ctgttgatgt
      121 gttgtttctt aatgccttga tagccagtat ctacctgtgt tttgatgttt ggatgcataa
      181 atattctgct gtttttaaaa agcttaaaat caataaatat aggtgtctca agagaattta
      241 tcctgaaaaa tgtccattac gccatatttt actacaagtt gtgagtcgca aaatatcctt
      301 acttttgcgc aatgattgaa tttaacagca tacaattaca caacggactt acacttattc
      361 accatgaaga caccactacg ccttttgttg tggtaaacac gttgtataaa gtgggtgcga
      421 aagatgaaga tgaacaccgc accgggtttg cccatttgtt tgaacatctg atgtttggag
      481 gaacaaagaa tttccctgat tttgataaac ctttgcaaga atctggcggc gaaaacaatg
      541 cgtttaccaa taacgattat accaattact atgatatcgt tcctgccgaa aatatagaaa
      601 taccactctg cctcgaagcc gacagaatga cgcaactcaa catcaataca aagagtttat
      661 ccgtgcaacg caaagtagtt tgtgaagaat ttaaagaaca ttacatcaat cagccttatg
      721 gcgatgtgtg gcatcttata cgagagatgg tgtatgaaaa acaccattat aaatggccta
      781 ctatcggcaa agaattgaaa catatcgaag aggctacatt agatgatgtg cgctcgttct
      841 atcaaaaata ttatcagccg ggtaatgcga ttttagtcat agccggaaat atcaactggg
      901 ataaaacaca agaactcgtt cagcattatt ttggtgcaat ttcttctact tccgttaaac
      961 acagtctgat tactgagccg gaacaaaccg ctgcaaaaac aaaaacagta cacgaagaag
     1021 tgcctttcag tgcgctgttt atcaacttta aaatggaaga caggctgaat aaagcctatt
     1081 atacagctga tatctgctca gatttattat cgaacgggac ggcctcgcgt ttgcaaaaaa
     1141 aattagtgaa agaaaaaaag gttttcatag agatagattg ctatatcagc ggcagtgtgg
     1201 aaacgggtat ctttactgtt gaaggaaaag taaaagagga tatcgacatg acgtatgccg
     1261 agcaattagt ttgggaagaa ctggaaaaac tgaaaacagg cgatgtacac gaaaatgagt
     1321 tgacaaaatg caaaaacaaa atgctcacct acatgaattt ctccgattcg agtttattga
     1381 atcgcagcat cagtttggca ttttatgaac ttttaggcga tgcccaactc atcaataaag
     1441 aagaagaatt ttacacgaat atcacttcgg cagatgtaca ggcttatgca aaatctttat
     1501 ttgtaaaaga aaaaagcaat acactgtatt atttgaaaaa ataaatctga ctaaaaaaaa
     1561 cgagcattat gaatctgcct cccatccacg atatacaaca aatacaacct gccttgcccg
     1621 acacgaaagt gcttgccaat gggatagttt tacacggctt taatgggtta aaaaacgaca
     1681 tcttgaaact ggatttggtt tttaatgccg gaaaatgggt cgaaccccaa caaatggtag
     1741 ccgaatttac agctcgctta ttcaaaagcg gaactactca gtttgatgcc catgcgctta
     1801 gtgaaaaaat tgaatttctg ggcagcagta taaaagccgg tgtgggctac aataccttta
     1861 cggtatcggt aagttgcatg aataaacatc tggaagccac cttagggttg ctgttaattt
     1921 gtttaaacga aattaatttt cctgaaaatg agattacaca cgaacagcag aaagccaaat
     1981 ctcaattaaa attagcgttg gaaaaaaacg actatatcgc cgatattaca ttccgtaagt
     2041 tgttgtttgg tgaaaatcat ccttatggtt atgcatcaac ggatgcgacc attgatgcca
     2101 ttgcccacaa atcactctta gattattaca acacatacgt gataccatcg ctttgcacac
     2161 tttatattgc cggaaaatat gccc
//
LOCUS       DAOQII010000327         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_39362_length_2171_cov_4.320416, whole genome shotgun sequence.
ACCESSION   DAOQII010000327 DAOQII010000000
VERSION     DAOQII010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39362_length_2171_cov_4.320416"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLF48_11600"
     CDS             complement(<1..675)
                     /locus_tag="PLF48_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNF50018.1"
                     /translation="MGKVLIIGAGGVGNVVVRKCAQVSEVFTDITLASRTKAKCDAIA
                     NDIKNVHVKTAQVDADNAQQVAELIRSTGAELVINVALPYQDLPIMDACLETGVHYLD
                     TANYEPKDEAKFEYSWQWAYQERFKAKGIMALLGCGFDPGQSQIYVAHAAKHHFDEMH
                     YLDIVDCNAGDHGQAFATNFNPEINIREITQDGKYWENGEWKIIPAMSIHKPIDYPNI
                     GPKESYV"
     gene            892..1365
                     /gene="greA"
                     /locus_tag="PLF48_11605"
     CDS             892..1365
                     /gene="greA"
                     /locus_tag="PLF48_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNF50019.1"
                     /translation="MTEIVYVTEEGLEKMKEELNHLMSVERPNASKAIAEAREKGDLS
                     ENAEYDAAKEAQGLLEVRIKKLQSDIGNSRILDPSKIDTSRVSVLSKVKVLNTKMKKE
                     FTYHLVSEKEADLKLMKISVKSPIGAALLGKVKGEKVNIQIPAGSMELQILDISV"
     gene            complement(1373..>2171)
                     /locus_tag="PLF48_11610"
     CDS             complement(1373..>2171)
                     /locus_tag="PLF48_11610"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNF50020.1"
                     /translation="FERKYFKLCEKSSFQNLIQSCRVLATWDDHDYGKNDGGKEYPQK
                     AMSKDIFMKFWGEENNTERQSHDGIYTSFYFGNDSQRIQIILLDCRTFRDKLAIDADG
                     NYIPQNNPDMTMLGEEQWAWLENELQKPAKIRIIGSSTQFARSYNGYETWANFPLEQQ
                     KMLAIIKRTQAKGLFFISGDVHLAELSKMSNPANYSIYDFTSSGLTEIEEVDIPNTNR
                     IGNSVFDSPNFGIITINWHPPVKIQFTIGTISNSKFLNYTINLSDLQ"
BASE COUNT          658 a          420 c          408 g          685 t
ORIGIN      
        1 cacatacgat tcttttggtc cgatattagg ataatctatc ggtttgtgta tggacattgc
       61 cggaatgatt ttccattcgc cgttttccca atattttcca tcttgcgtaa tttcgcggat
      121 attgatttca ggattaaaat ttgtagcaaa tgcttgtcca tggtcgccgg cattgcaatc
      181 tacgatatcc agataatgca tttcgtcaaa atgatgcttg gcagcatgtg ctacatatat
      241 ttgtgattgt cccgggtcga atccgcaacc caataaggcc ataatacctt tagctttaaa
      301 tcgttcctga taagcccatt gccagctgta ttcaaatttg gcttcgtctt taggttcata
      361 atttgcagta tccaaataat gcacacccgt ttcaaggcaa gcatccataa taggtaaatc
      421 ctgatatggc aatgccacat tgatgactag ctctgctccc gtggaccgta taagttctgc
      481 cacttgctga gcattatctg catccacctg agctgttttc acatgtacgt ttttgatgtc
      541 atttgctatc gcatcacatt tggcttttgt tcggcttgcc aaagtaatat ctgtaaatac
      601 ttctgaaact tgagcgcatt tgcgtaccac tacattgcct actccacctg caccgataat
      661 caacactttt cccatttttt tacctgatta aattatgaat aataaaagtt tgcgatgcaa
      721 aaatacaaaa tagagtatga catttcttgt ttttgatgtc tggattttta agaaataagg
      781 aaaaataaaa aacacttgac attttcaaaa aaaggtttaa ttttgagcat gattcagtgg
      841 cacgttccca aaaggaacgt gtcgtttttt ttcaatttaa atagaaataa gatgacagaa
      901 atcgtgtatg ttaccgaaga aggcttggaa aaaatgaaag aagagctcaa tcatttgatg
      961 agtgtagaac gtcctaatgc atccaaagcc attgctgaag cccgtgaaaa aggcgactta
     1021 tctgaaaatg cggaatatga tgccgctaaa gaggcacaag gattattaga agtaagaata
     1081 aagaaactac aatcggatat aggcaattcc cgcatattag acccgagcaa aattgataca
     1141 tccagagtgt ctgtattatc gaaagtgaag gtgctgaata ccaaaatgaa aaaagaattt
     1201 acttatcatt tagtatcgga aaaagaagcc gatttaaaat tgatgaaaat atcagtgaaa
     1261 tctcccatcg gtgcggcttt attgggcaaa gtaaaaggtg agaaagtgaa tatacaaatt
     1321 ccggcaggca gcatggaatt gcagatactg gatatcagtg tatagctttt ttctactgca
     1381 aatcagataa attaatcgtg taattcagga atttactatt gctgatggtg ccaatggtaa
     1441 attgaatttt caccggagga tgccagttaa tggtgattat tccaaaattt ggactatcga
     1501 atacagaatt acctatacga ttagtatttg gaatatctac ttcttcaatc tccgtcaaac
     1561 cgctggaggt aaaatcataa atggaataat tagctgggtt gctcatcttg gataattctg
     1621 ccaaatgcac atctcccgat ataaaaaaca gacctttagc ttgtgtacgc ttgatgatag
     1681 ccagcatttt ttgttgttcc aacgggaaat ttgcccatgt ttcgtagccg ttgtaggagc
     1741 gggcaaattg cgtactgctg ccaattattc tgatttttgc aggcttctgc aattcatttt
     1801 ccaaccatgc ccactgctcc tctcccagca tcgtcatatc cgggttgttt tgcggtatat
     1861 aatttccatc cgcatctatt gccagtttat ctctaaacgt gcgacaatct aacaagatga
     1921 tttgtatacg ctgactgtca ttgccaaaat aaaaggatgt ataaatccca tcgtgagatt
     1981 gcctctcggt attattttct tcgccccaaa atttcatgaa gatatctttc gacattgcct
     2041 tttgcgggta ctctttgcca ccgtcatttt tgccgtaatc atggtcatcc catgtagcca
     2101 aaactctaca agactgaatt aaattttgga aagaagattt ttcacaaagt ttgaaatatt
     2161 tccgttcgaa a
//
LOCUS       DAOQII010000328         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_39860_length_2157_cov_4.535680, whole genome shotgun sequence.
ACCESSION   DAOQII010000328 DAOQII010000000
VERSION     DAOQII010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39860_length_2157_cov_4.535680"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLF48_11615"
     CDS             <1..543
                     /locus_tag="PLF48_11615"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNF50021.1"
                     /translation="YYNTMSDTKKYLVGIFNDDDILLHAVKGIRKDGYEISNAYTPFP
                     VHGLEHALGLEETRLHTAGFFFGATGLTFALSMITFINKFNYPTNVGGKPYWGLLAYV
                     PITFEQTVLAAAVGMTVSFYYLCKLYPGKKPKIVDERTTDNMFALTFELDAAKDNAYV
                     NAVTELLKKEGAIEVYQKEV"
     gene            545..1135
                     /locus_tag="PLF48_11620"
     CDS             545..1135
                     /locus_tag="PLF48_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF50022.1"
                     /translation="MSHKSFIILFAAVAVLASCIRSPKKPGRQYMPDMVNSIAYDAGS
                     PNPVFKDGKTNQLPPVGSVARGTYTYPLSDTEYARAATAITNPFTFTKDEIEGNGKHL
                     FTVNCAICHGDKGDGQGHLVQINKFPAPPSYFSDILMTKPEGQRYHSLMYGKGLMGSY
                     ASQLNHRERWLVLAYVKALQDASKGGSASTDSTITK"
     gene            1159..>2157
                     /locus_tag="PLF48_11625"
     CDS             1159..>2157
                     /locus_tag="PLF48_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF50023.1"
                     /translation="MNNNQYVLDAGTQKNIFIAFAIGVVLLILGVLFTNAGKKEAHEE
                     HGTKTEHVVTENAAHEAVAEHKGGHHAEEVTVKEVVMFNAYNVFYFGFYIALAALFFL
                     AGATLAWGGWQIQIQKIPLAMASNIGLFLVLLFVMFAVFGHDIFEWMHEDLYNPASPA
                     YDPILASKHDFLNPTSFWVFSIIIATSCVLMFMKWWKTLTGMDSNPSRKLFSSSRMIA
                     AISIVIISFVINTFATWYWSMSIQPHWYSTMFTWNTMAGAAVTMFSIVILLMNFLKSK
                     GYLPRVNANHEHDVAKLMFAISVFWTYTWFAQYMLIWYANIPEETIFFQIRRTTGTY"
BASE COUNT          667 a          353 c          440 g          697 t
ORIGIN      
        1 tattataata ctatgagcga tacaaaaaaa tatttagtcg gcatttttaa tgatgatgac
       61 atcttattac atgctgtaaa aggtatcaga aaagatggct acgaaatttc aaatgcctat
      121 actccatttc cggtacatgg cttggaacat gcattaggct tggaagaaac cagattgcat
      181 actgcaggct ttttctttgg agctacaggt ttgacattcg cattaagtat gataactttt
      241 atcaacaagt ttaactatcc gacaaatgtg ggtggcaaac cgtattgggg tttattggct
      301 tatgtgccta ttacctttga acaaacggtg ttggccgcag ctgtgggtat gacagtttcc
      361 ttttattact tgtgtaagtt atatccgggt aaaaaaccga aaatcgtaga tgaaagaact
      421 acggataaca tgtttgcatt gacatttgaa ttagatgctg ctaaagataa tgcctatgta
      481 aatgcagtga cagaattatt gaaaaaagaa ggtgcaattg aagtatatca aaaagaagtt
      541 taacatgtct cataaatcgt ttatcatttt gtttgcagct gttgcggtat tggcttcttg
      601 tattagaagt cctaaaaagc cgggcagaca gtatatgcct gatatggtta actctatagc
      661 ctatgatgca ggttctccaa acccggtatt taaagatggc aaaactaatc agttgccacc
      721 tgtgggcagt gtagccagag gtacttatac ttatccttta tctgatacag aatatgccag
      781 agctgctaca gcaataacca atccttttac ttttactaaa gatgaaattg aaggaaatgg
      841 aaaacatttg tttaccgtaa actgtgcaat ttgccacgga gataaggggg atggtcaggg
      901 acatttggtg caaatcaata aatttccagc accgccatct tatttttcag atatattaat
      961 gacaaaacct gaagggcagc gttatcattc gctaatgtat ggtaaaggct tgatgggatc
     1021 gtatgcttct cagcttaatc acagagaaag atggttggta ttggcttatg tcaaagcctt
     1081 gcaagatgca agcaaaggcg gaagtgcatc aacagattca actattacta aataaaaatc
     1141 tataaatttt cgattgcaat gaataataat caatatgttt tagatgctgg cacgcaaaaa
     1201 aatatcttca tcgcatttgc aataggtgtg gtacttttaa tattaggggt gttatttacc
     1261 aatgcaggta aaaaagaagc acatgaagag catggtacaa aaacggaaca tgtagttact
     1321 gaaaatgctg cacacgaagc tgttgctgaa cacaaaggtg gtcatcatgc tgaagaagta
     1381 acagtgaaag aagtcgttat gttcaatgcg tataacgtat tttacttcgg tttctatatc
     1441 gctttggctg ctcttttttt cttggcgggt gctactctgg catggggtgg ctggcaaata
     1501 cagattcaga aaattccttt agctatggca agcaatatag gcttgttctt agttttgtta
     1561 tttgtgatgt ttgctgtttt cggtcatgat atttttgagt ggatgcatga agatttatat
     1621 aatcccgcat caccagctta tgacccgata ttagcttcaa aacatgactt ccttaatcct
     1681 acatctttct gggtcttcag tatcattata gctacatctt gtgtgctgat gtttatgaag
     1741 tggtggaaaa cattaacagg aatggatagc aatccgtcca gaaaattatt ttcatcatca
     1801 cgtatgattg cagcaatatc tatagttatt atttcttttg tcattaatac atttgctact
     1861 tggtattggt caatgtctat tcagccacac tggtatagta ccatgtttac atggaataca
     1921 atggcaggtg ctgccgtaac gatgttttca attgtaattt tattgatgaa ctttttgaaa
     1981 tcaaaagggt atttaccgcg agtaaatgct aaccatgaac atgatgttgc caaattgatg
     2041 tttgctatca gcgtattctg gacttatact tggtttgcac aatacatgtt aatttggtat
     2101 gcgaatattc cggaagaaac tatatttttc caaatcagaa gaacaactgg cacttat
//
LOCUS       DAOQII010000329         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_40295_length_2144_cov_3.449976, whole genome shotgun sequence.
ACCESSION   DAOQII010000329 DAOQII010000000
VERSION     DAOQII010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40295_length_2144_cov_3.449976"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLF48_11630"
     CDS             complement(<1..492)
                     /locus_tag="PLF48_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HNF50024.1"
                     /translation="MKIAFLLNATIRKKEKVIAEVKQTFSKHEIEFFESEYAGHFFSL
                     PQLILDKGFSILIVIGGDGTLNEVINGLIQACKLSADSIDWKRIATIKIGVYAAGSGN
                     DFIKTVYKDNQLYSLKKLIENESSQLIDIGYVTFFDPSNNPASRYYMNITDVGMGGEA
                     VMLK"
     gene            complement(524..2080)
                     /locus_tag="PLF48_11635"
     CDS             complement(524..2080)
                     /locus_tag="PLF48_11635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNF50025.1"
                     /translation="MYRINNIQKAKSELFDVVIIGGGITGAGIALTAARKGWKVLILD
                     KADFAYGTSSRSAKMIHGGLRYLQQLQIKLVKEALQEREHLLNEYPHLVKPQPFFMPI
                     YKSKLTKLKLGIGLSGYDYLSGISSMPKHAEVDLEEIKMRFPQIKSESLVGGFIYYDA
                     KTNDARLVNEVMQEAADLGVVVLNYLELTAFHHEKNKIKQISCKDKISGQSIEIKSKV
                     FISATGVWTDDLMKLLNPADNKKYMAPSKGIHLIVSADFFPKDCVMLLPTSNGDGRML
                     WCMPWDDNLNVIGTTDTDYSGSLDMMHTDVDEVQYILDSINAQLNDKQITKDDILSVY
                     AGLRPLINDNDESKKSNQRSRDYQIWWNNENMLTISGGKLTSFLSMAENCIKTIEEKY
                     KIESLEKDEILFSSYIDMYKHRYGIKNATLIANIIQESAEKLNDTKFSYPYTDAEFIF
                     FIRHQFVQKIDDLLTRRTLVTYQMQQFDEVFVQYIADLMAKELNWNEERKTSEINAYR
                     IEWQVMHSWK"
BASE COUNT          634 a          424 c          350 g          736 t
ORIGIN      
        1 tttgagcatt actgcttcgc cacccatacc cacatcggtt atattcatgt aatatctgga
       61 agcaggattg tttgaagggt cgaaaaatgt aacatatcca atatctataa gttgcgacga
      121 ttcattttca ataagttttt taagagaata aagttgattg tctttataca ctgttttaat
      181 aaaatcgttc ccgctcccgg ctgcataaac tccaatttta atcgtggcaa ttcgtttcca
      241 gtcaatggag tcagcggaaa gtttacaagc ttgtattaac ccgttaatga cttcgttgag
      301 cgtgccatcc ccaccgataa ctatcaaaat ggagaaccct ttatctaata ttaactgagg
      361 tagtgaaaaa aaatgacctg catattcaga ttcaaagaat tcaatttcat gtttgctgaa
      421 agtctgtttt acttcagcaa ttactttttc ttttttgcga attgtggcat ttaacaagaa
      481 ggcaattttc ataaggtgtt ttttttactg caacaacatc aacttacttc cacgaatgca
      541 taacttgcca ttctatacgg tatgcattta tttcactcgt ttttctttct tcattccaat
      601 ttaactcttt tgccattaaa tctgcaatat attgaacaaa aacttcatca aattgttgca
      661 tttggtaagt aaccagtgta cggcgagtga gcaaatcatc aattttttgt acaaactgat
      721 ggcgaatgaa aaagataaat tccgcatcgg tataaggata agaaaattta gtatcattaa
      781 gtttttctgc agattcctga atgatatttg ctatcagtgt ggcattttta atgccatatc
      841 ggtgcttata catgtcgata taagatgaga ataaaatttc atctttttcc agcgattcaa
      901 ttttatattt ttcttcaata gtctttatac aattctccgc catcgacaaa aatgaagtga
      961 gtttgccacc cgaaatggta agcatatttt cgttattcca ccatatctga taatcgcggg
     1021 aacgctgatt gctcttcttg ctttcgtcat tatcattaat taaaggacgt aagcctgcat
     1081 agacacttaa tatatcatct ttagttattt gtttgtcatt tagctgcgca ttgatgctat
     1141 cgagaatata ctgtacttca tcaacgtccg tatgcatcat atccagtgat ccgctgtaat
     1201 ctgtatcggt agtacctatt acattcaggt tatcatccca gggcatacac cacaacattc
     1261 tgccatcgcc attgctggtt ggcaaaagca tcacacaatc tttcgggaaa aaatcggcag
     1321 aaactatcaa atgaatacct ttgctgggag ccatgtattt tttgttatca gcaggattca
     1381 acaatttcat caaatcgtcg gtccatacac ccgtagcaga aataaatact tttgatttta
     1441 tttcaataga ttgacctgaa attttatcct tacagctgat ttgttttatt ttattctttt
     1501 catgatgaaa tgcagtcagc tctaaataat taagtacgac tactcctaaa tctgcggctt
     1561 cctgcatcac ttcgtttact aatcgcgcat cgtttgtctt ggcatcatag tagataaacc
     1621 caccaaccaa actttcggat ttgatttgtg gaaatcgcat ttttatctct tccaaatcta
     1681 cttcggcatg tttgggcata ctgcttatac cagaaagata atcgtagcct gaaagtccga
     1741 tacccaattt caacttcgtc agtttagatt tgtatattgg cataaaaaat ggttgcggct
     1801 tcactaagtg cggatattca ttgagcaaat gttcccgttc ctgcaaggct tctttcacca
     1861 gttttatctg taactgttgt aaatagcgca aacccccgtg aatcattttt gcagaccgcg
     1921 aactggtgcc ataggcaaaa tctgctttat caagaatgag cactttccaa ccttttcgtg
     1981 cagccgttag tgcaatacct gcacctgtaa tgccacctcc gataattact acatcaaata
     2041 attcagattt tgctttttgt atgttgttga tgcggtacat aatgaatgat gaatgttgaa
     2101 tgtttaaatt acaaattacg aattacaaat tacaaattac gaat
//
LOCUS       DAOQII010000330         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_42049_length_2095_cov_3.790196, whole genome shotgun sequence.
ACCESSION   DAOQII010000330 DAOQII010000000
VERSION     DAOQII010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42049_length_2095_cov_3.790196"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLF48_11640"
     CDS             complement(<1..269)
                     /locus_tag="PLF48_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HNF50026.1"
                     /translation="MKQLKISTKITSRDSFSIDKYLSEISKLPMLSEEEEAECARKIR
                     EGDTRAEDKLVQANLRFVVSVAKQYQNQGLALNDLINEGNIGLIKA"
     gene            complement(468..1982)
                     /locus_tag="PLF48_11645"
     CDS             complement(468..1982)
                     /locus_tag="PLF48_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF50027.1"
                     /translation="MSTFFTADHEQFRQTIRQFLQTEAAPFIDQWEADGKIPREFWKK
                     FGEMGYFGLSMPEQYGGSGLDFFYEVVMLEEASKMYSGGFTITAAVQTCMSTPYILHH
                     GSEYLKETFLKPAIAGDMIGCIGITEPGAGSDAANIQLRAKKEGDYYILNGTKTFITN
                     GIYGHFVILVCKTNPDAGSAGVSLILVDLSTEGITKNKLKKLGWHASDTAELHFDNVR
                     VPAKNLLGEEGKGFYYLMGGLQLERLAGSIMGYASCEEILSYSMQYMNERSAFGRTIN
                     KFQVLRHRVAQLAAEIECVKTFVLTTAKQHTEGKYVVKECSMAKLLSTELADKCMYQC
                     VQFFGGYGFMEEYKVARAYRDSRIGTIGGGTSEIMREIIAKMVMDDINYKSSVARQPA
                     EEANQFNTVADIFSTLPSRFKSEKAKGKKLNVAFNFDHANYFVLIDDGKLDIQSGVPN
                     IAEAVNCTVETDDATYIAVETGKLNPQEAFMTGKIKVSDIMSMMEFGGLFKKLT"
BASE COUNT          629 a          421 c          366 g          679 t
ORIGIN      
        1 gcctttatca atccgatatt gccttcattt attaaatcat tcaatgccaa accttgattt
       61 tgatattgct tggccacgga aacaacaaat cttaagttag cttgaactaa tttgtcttca
      121 gcccgggtat caccttctct gattttgcgt gcgcactctg cttcttcttc ttcacttagc
      181 ataggcaatt tggatatctc actgagatat ttatcgatag aaaaactatc gcgtgaggtg
      241 attttggtgg aaattttgag ttgcttcatg ttgttaggct tacaccaaga tagtaagaat
      301 ttagtttaga tgtatgtaat taaaaaaaaa gataaataga atgctctatc tttctgaata
      361 caaatatgat ataatcaatg tctgaattgg tgctgatata ataatgaaac atgaatttat
      421 gcttcaagac aaaaaatgat atttcagaaa atttaaatgc taattgacta agtcaatttt
      481 ttaaataaac caccaaattc catcatactc ataatatcac ttacttttat ctttccggtc
      541 atgaacgctt cctgaggatt gagttttccg gtttctactg ctatataagt agcatcatca
      601 gtttccacag tgcagtttac agcttctgct atattcggta ctccggattg gatgtctaat
      661 tttccatcat caattaaaac gaaataattt gcatgatcaa aattaaaggc cacattcagt
      721 tttttacctt tagctttttc tgatttaaac cgggatggga gcgtggaaaa gatatccgct
      781 acagtattga attgattagc ctcttctgcc ggttgccttg caactgatga tttataatta
      841 atgtcatcca tcaccatttt tgcaatgatt tcccgcataa tttctgaagt tccgccacca
      901 atagtaccaa tacgtgaatc gcgataagca cgagcaactt tatactcttc cataaatccg
      961 taaccaccga aaaactgaac acactgatac atacatttat ctgcaagttc agtagaaagc
     1021 aatttagcca tcgagcattc ttttaccacg tactttccct cggtatgttg tttggctgtg
     1081 gtgagtacaa aagtttttac gcattcaatt tcagcggcta attgtgccac acgatgtcgc
     1141 aatacctgaa atttatttat cgttctgcca aaggcacttc gctcattcat atattgcatg
     1201 gagtagctta gtatttcttc gcaagaggca tagcccatga tagaacctgc taatcgctct
     1261 aattgcaaac cacccattag atagtagaag cctttgcctt cttcgcccaa caaattttta
     1321 gcaggtactc gtacattgtc aaaatgaagc tctgccgtat cagaggcgtg ccagcctagt
     1381 tttttaagct tgtttttggt aattccttca gtagataaat cgacgagaat caggcttact
     1441 cctgcagatc cagcatcggg attggttttg cataccagaa taacaaaatg cccgtaaata
     1501 ccattagtga tgaatgtttt tgttccgttc aagatataat aatcaccctc tttttttgcc
     1561 cgcaactgga tattcgctgc gtcactgccc gcaccgggct ctgtgatacc gatacaacct
     1621 atcatatcgc cggcaatagc aggtttcaaa aaagtttctt ttagatactc gctgccatga
     1681 tgcaaaatat agggtgtact catacatgtt tgcacggctg cagtaatggt aaagccacca
     1741 ctgtacattt tagaagcttc ttcgagcatg accacttcat agaagaaatc gagtccgctg
     1801 ccaccatatt gttcaggcat agataaaccg aaataaccca tttcgccgaa ttttttccaa
     1861 aattctcgcg gtatttttcc gtcagcttcc cattggtcaa taaagggagc cgcttcagtt
     1921 tgtaaaaatt gtcggatggt ttgtctgaat tgttcgtgat cggcagtaaa gaaagtactc
     1981 ataaacgctg tcattttttt aaatgggaat aagaccaaaa ataacaaaat aagaagaaga
     2041 aagaattatc ttttgaaaaa attgtgtaaa tagtacattt aataggatag tggtt
//
LOCUS       DAOQII010000331         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_43263_length_2066_cov_3.892591, whole genome shotgun sequence.
ACCESSION   DAOQII010000331 DAOQII010000000
VERSION     DAOQII010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43263_length_2066_cov_3.892591"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1135)
                     /locus_tag="PLF48_11650"
     CDS             complement(293..1135)
                     /locus_tag="PLF48_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF50028.1"
                     /translation="MKKTQNIKINGLHQRPITLDIFSHEDADKSPLVLFIHGFKGFKD
                     WGHFNLIADAFVKAGFSFVKFNFSHNGTTPDALEDFADLEAFGQNNFTMELDETKLVI
                     DYLEQQASSSHYDFEKLYLIGHSRGGGIAVLTAFEDKRVKKLITWASVASFDNLFKSI
                     DTDKWKNDGVVYSFNSRTKQDMPLYYQLYEDFRANADRLNIVQAASQLSIPWLIIHGK
                     SDTSVAVRSAEQLHACQPKSELFLLEDAEHTFGGKHPYSDEHLPNDTALLIQKSVQFL
                     IASS"
     gene            1191..>2066
                     /locus_tag="PLF48_11655"
     CDS             1191..>2066
                     /locus_tag="PLF48_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNF50029.1"
                     /translation="MSSVVDKIKQPIEKELELFEKKFRDDLRSHVPLLDKVNYYIYQR
                     KGKQIRPMFIFLTAQMCGGVTENTYIGASLVELLHTATLVHDDVVDDAYERRGFFSVN
                     ALWKNKIAVLVGDYFLSKGLLLALDSKNYNLLHITSNAVKKMSEGELLQMEKARRLDI
                     TEDVYYEIIRNKTASLISSACEVGAASATNDEQVLRQMSLMGEYIGMAFQIKDDLFDF
                     GEADIGKPRGIDIKERKMTLPLIYTLNNCSAKDKKWIINVVKNHNEDKAKVNELIHFI
                     NTHGGIAYTEKKMLEY"
BASE COUNT          690 a          388 c          372 g          616 t
ORIGIN      
        1 agaccggagc tgcttttttc ttcgttggca tatgcaaaaa tactaaataa aattagtaat
       61 atagtatatt aatattaagt atttataaac aattattcat aaaaaaagac tgccctttta
      121 agacagcctc ctcttgataa gactaaattt gcaccactac taaactaatc caccaatcaa
      181 aggtttggcc actacgtggc atctttgatt atcagtttaa taaaaaggaa atattactaa
      241 gcacacacat cgtaaactcc actcagataa acgtaagaat ggataaacct acttaggacg
      301 aggcaatcaa aaactgtaca cttttctgaa ttaacaatgc ggtatcattt ggtaagtgtt
      361 catcactata aggatgcttg cctccaaaag tatgttccgc atcttctagt agaaacaatt
      421 cacttttggg ttgacatgca tgcaattgtt ccgcacttct tacagcaacc gaagtatcac
      481 tcttgccatg aataatcaac cagggtatcg aaagttggct tgccgcttgt acaatattga
      541 gtcggtcagc atttgcccgg aaatcctcat aaagctgata atataagggc atatcttgtt
      601 ttgttctgga attgaaggaa tatacaactc catcattttt ccacttatcc gtatcaatgc
      661 tcttaaataa attatcgaaa gaagcaacac ttgcccaagt aatcaatttc ttgacacgtt
      721 tatcttcaaa cgccgtaagc accgcaatgc ctccaccgcg gctatgacca atcagataca
      781 atttttcaaa atcataatga gaactactag cttgttgctc taaataatct ataaccaatt
      841 tggtttcatc caactccata gtaaaattat tctgaccaaa ggcttccaaa tcggcaaaat
      901 cctcaagcgc atctggtgtg gttccattat gagaaaaatt gaatttgacg aaactaaatc
      961 cagcctttac aaaagcatct gctataaggt taaaatgtcc ccaatcttta aaccctttaa
     1021 acccgtgaat aaataatact aaaggcgatt tatcggcatc ctcatgactg aaaatatcca
     1081 atgttatagg tctttgatgc agtccgttta tctttatatt ttgtgttttt ttcatcttgc
     1141 ttatttagtc gaattggtaa atatatttac ctttaagcaa aatttaaaac ttgagcagtg
     1201 ttgttgacaa aataaaacag cctatagaaa aggaactgga attgtttgaa aaaaaattcc
     1261 gggatgactt gcgcagtcat gttccactgc tcgacaaagt aaattattat atctatcagc
     1321 gaaaaggaaa acaaattcgc ccgatgttca tctttcttac ggcacagatg tgtggcggag
     1381 taacggagaa tacgtatatc ggggcatctt tggtagaact gctgcatacg gctacattgg
     1441 tacatgatga tgtggtggat gatgcgtatg agcgtcgcgg gtttttttct gttaatgccc
     1501 tttggaaaaa taaaattgcc gttttggtgg gcgattattt tttgtcgaaa ggtttattac
     1561 tggcattaga ttctaaaaat tacaatctcc tgcatattac ttccaatgcc gtaaaaaaaa
     1621 tgagcgaagg cgagcttttg caaatggaaa aagcccgtcg cttggatatt accgaagatg
     1681 tttattatga aatcatccgc aataaaacag catctctgat ttcatctgcg tgcgaagtag
     1741 gtgcagcctc ggcaaccaat gatgaacagg tattgcgaca aatgagtttg atgggcgagt
     1801 atatcggcat ggctttccaa attaaagatg atttatttga ttttggcgaa gctgatatcg
     1861 gcaagcccag aggaatagat attaaagaac ggaagatgac tttaccactc atttataccc
     1921 tgaataattg ctctgcaaaa gacaaaaaat ggattattaa cgtggtgaaa aatcataatg
     1981 aagacaaagc aaaagttaac gagttaatac actttataaa tacacatggc ggcatagcct
     2041 acacggaaaa gaaaatgctc gaatac
//
LOCUS       DAOQII010000332         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_43332_length_2064_cov_3.936287, whole genome shotgun sequence.
ACCESSION   DAOQII010000332 DAOQII010000000
VERSION     DAOQII010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43332_length_2064_cov_3.936287"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..>2064
                     /gene="yidC"
                     /locus_tag="PLF48_11660"
     CDS             188..>2064
                     /gene="yidC"
                     /locus_tag="PLF48_11660"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNF50030.1"
                     /translation="MNFDKNTVIGIALIVLIYFGFVVYTNKEKADYEKAHPKTAATAQ
                     HDSIAATAKDTLQAASNSKPTGSDSAKSTVAGTFGAFASGIEKTIDIENDNCIFTFSN
                     KGGVIKKVNLKKYKTFSKQPLILFDDNGNGFNIEFNAQGNPVQTEQLYFESNNASQVV
                     TGDGKTEIIYTLNLGNGKTYQHKYTVYAKGYVADFELIANNLGSELGNTKQLTLDWAQ
                     NYPSQERGIDDQRYNSGVYFMNDKEEDDSYTSDKDKTIEEPLKWISYKQKFFNSSLIA
                     VKDNFGANTQLNIVTPKDSQAIIKEVKSKLFIPVKNPNAISFPVKMFLGPNKYNDLKK
                     LDVNLTQIIPLGWSILSLINKYFIIPIFHFLSKFISNYGIIILLLAVIIKLITFPFTY
                     KSSMSMAKMKVLKPELEELKKKYPNQAEYGQKQMELYAQTGVSPFGGCLPMLLQMPIL
                     IAMYRFFPASIEMRQQSFLWAQDLTSYDSIFSWTTHIPVISSLLGNHISLFTILMAVS
                     SIAVTKLTQQTQPMSGGGGGGGMEDMMAQQMKLMQYFMPIMLLFIFNKQPAALSYYYF
                     LFNVLTVGQNWILQKFFIDEDSIKAKIEAYRKQPKKTSSFQQKMQDMMNFPVVDGYTV
                     KL"
BASE COUNT          722 a          375 c          351 g          616 t
ORIGIN      
        1 cacaaaactg cgtcttaaac agagataaat taaaatttaa gtaacaaaat attggctatc
       61 tgccattttg gggtttcaag ttgatctatt tttaaaataa tgcctcaaat taacctattt
      121 tatttaataa ccttaatcta ccctaaaatt catatccttt tagtagcttt gcgcttcaat
      181 ttttactatg aattttgaca agaatacggt tattggcatt gcactgattg ttttaatata
      241 ttttggtttt gttgtttata cgaataaaga aaaagccgat tatgaaaaag cacatcctaa
      301 aacggctgcc acagctcagc atgattccat tgctgccact gcaaaagata cactacaggc
      361 tgcaagtaac agtaaaccta caggaagtga ttctgccaaa agtacggttg cgggaacatt
      421 tggtgctttt gcttccggca tagagaaaac aatcgacatc gaaaatgaca attgcatttt
      481 cactttctcc aataaaggtg gggttataaa aaaagtaaat cttaaaaagt ataagacgtt
      541 ttccaaacaa ccgcttattt tatttgatga taatggcaat ggttttaata tcgaattcaa
      601 tgcacaagga aatcctgtgc aaaccgaaca gctttatttt gaatcaaata atgcctctca
      661 agttgtgacc ggcgacggta aaacagaaat tatctataca cttaacctgg gtaatggcaa
      721 aacttaccaa cacaaataca ccgtgtatgc taaagggtac gtagccgatt ttgaacttat
      781 tgcgaataac cttggatcag aactaggcaa tacaaaacaa ttgaccttag actgggcaca
      841 aaattacccg tcgcaagaaa gagggataga tgaccagcgg tataattccg gcgtttattt
      901 tatgaatgac aaagaggaag atgacagcta cacgtctgat aaggataaga ccattgaaga
      961 acctttaaaa tggatttctt acaaacaaaa atttttcaat tccagcttaa ttgccgtaaa
     1021 agataatttt ggtgccaata cacaattaaa tatcgtaaca cctaaagatt cgcaagcgat
     1081 aattaaagaa gtaaaatcaa aactatttat tcccgttaag aatccaaatg caatttcctt
     1141 tcctgttaaa atgtttttgg gaccgaacaa atacaatgac ttaaaaaaat tagatgtcaa
     1201 cctcactcaa atcatcccat taggctggag tatactgagc ttaatcaaca agtattttat
     1261 tattccgatt ttccattttc taagtaaatt tatttccaac tatggaataa ttatcctgct
     1321 tttagctgta atcataaagc tcattacttt tccatttacc tacaaatcat cgatgtcgat
     1381 ggctaagatg aaagtactga agcccgaatt ggaagaattg aaaaagaaat atcctaatca
     1441 ggctgaatat ggacaaaaac aaatggaatt atatgctcaa acaggtgtaa gtccatttgg
     1501 cggatgcttg cccatgcttt tgcaaatgcc gatattaatt gcgatgtacc gatttttccc
     1561 ggcatcaata gaaatgagac aacaatcttt tttatgggca caagacttaa caagttatga
     1621 ctcaattttt agctggacaa cacatattcc agtcatttca tcactcttag gaaatcacat
     1681 cagcttattt accattttga tggcggtttc ttcaattgcc gttacaaaac ttacgcaaca
     1741 aacacaaccc atgtcaggcg gcggcggcgg cggcggcatg gaagatatga tggcacagca
     1801 aatgaagctg atgcagtatt ttatgccaat catgctcttg tttattttca ataagcaacc
     1861 cgcagcgttg agttactact attttctgtt taatgtatta acggttggac aaaactggat
     1921 tttgcagaaa ttctttattg atgaagattc aataaaagca aaaatagaag cttacagaaa
     1981 acaacctaag aaaaccagtt cgtttcagca gaaaatgcaa gacatgatga attttcctgt
     2041 agtagatggt tataccgtaa aact
//
LOCUS       DAOQII010000333         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_47783_length_1963_cov_3.550314, whole genome shotgun sequence.
ACCESSION   DAOQII010000333 DAOQII010000000
VERSION     DAOQII010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47783_length_1963_cov_3.550314"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLF48_11665"
     CDS             complement(<1..261)
                     /locus_tag="PLF48_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNF50031.1"
                     /translation="MIYLTRKETFNAAHRLYNEHWSDEQNFEVFGKCANKNWHGHNFN
                     LFVTVKGIPNPETGFVINVKELGKIIKEKIILRLDHTNLNLDD"
     gene            336..1010
                     /gene="mqnB"
                     /locus_tag="PLF48_11670"
     CDS             336..1010
                     /gene="mqnB"
                     /locus_tag="PLF48_11670"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNF50032.1"
                     /translation="MRITLVAATQMEVQPLLDFFAAEKTEKSDLWKVDDNLHILITGM
                     GMLNTAAHLAIYTQQHDRDFYIDAGVCGAFNRNFPIGQVYQIISETYGDFGVENDDTF
                     EDFFDLGFLKKKEDAFQYGHCAPIKNHVHDIINLPVASAITVNKVHGNVQTIKIIQEK
                     YQADTENMEGLAFYYVLKMVNKPGIEIRAVSNYVEKRNKENWDLKGSVANLNTYLIQL
                     LQTLRL"
     gene            1080..1727
                     /locus_tag="PLF48_11675"
     CDS             1080..1727
                     /locus_tag="PLF48_11675"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNF50033.1"
                     /translation="MKTRLFFLFSTLTIFVFHAYAQNATDKYDTITLPRMVIGDDTLE
                     QIVYEPVIIYANRLEKANLPDWQMRYLRIVYPYALRTARVAKKVDEELAQMDKKRDKK
                     KYLNDCEKVLREQFENTLKNLTRKQGQMLILLINRETGRTVYDLLSDYRSGFKAFWWN
                     VIGKMYDLDMKAVYEPNGKDKEMEKYVLFLEDVYKVDGTKYIIDNEDVNIPNSNK"
     gene            1699..>1963
                     /locus_tag="PLF48_11680"
     CDS             1699..>1963
                     /locus_tag="PLF48_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNF50034.1"
                     /translation="MSIYQIPINNAKGKPLNWDDYKGKVLLIVNTASKCGLTPQYDDL
                     EKLYQEYKDRGFEIIGCPSNDFAGQEPLDSDKAEEFCKLNYGVT"
BASE COUNT          675 a          336 c          396 g          556 t
ORIGIN      
        1 atcatccaga ttcagattcg tgtgatctaa tcgcagtatg attttttctt tgataatttt
       61 gcccagttct ttcacattaa tcacaaatcc agtttcggga ttaggaatgc cttttaccgt
      121 cacaaataaa ttaaaattat gcccatgcca gtttttattg gcacattttc cgaatacttc
      181 aaaattttgc tcatcagacc aatgctcgtt atataaacga tgtgcggcat tgaaagtctc
      241 ttttctggtc aggtaaatca ccctgcaaag atagacatct aagttactat cccataaaca
      301 ggctgctatt cttaataaaa attttaattt tgcacatgcg tattacctta gtagcggcta
      361 cacaaatgga agttcagcca ttgctggatt tttttgctgc cgaaaaaacg gaaaaatcag
      421 atttgtggaa agtagatgac aacctgcata ttctgataac gggaatgggt atgctcaata
      481 cggcagcgca tctggcaatt tacacccaac aacacgaccg tgatttttat attgatgccg
      541 gagtttgcgg ggcattcaac cgcaacttcc ccattggtca agtttatcaa atcatttctg
      601 aaacctatgg tgattttggt gtagaaaatg atgatacgtt tgaagacttt tttgatttgg
      661 gatttttgaa aaaaaaagaa gatgcatttc aatatggaca ttgtgcgcca ataaaaaacc
      721 atgtgcatga cattataaat ttgccagttg ccagtgcgat taccgtaaac aaagtacacg
      781 gcaacgtgca aaccataaaa atcatacagg aaaaatatca ggcggataca gaaaatatgg
      841 aaggcttagc gttttattat gtactaaaaa tggttaataa acccggcata gaaataagag
      901 cggtatccaa ttatgtagaa aaaagaaata aagaaaactg ggatttaaag ggctcagttg
      961 ccaatctgaa tacttatttg atccaacttt tacaaacgct gcggttgtaa tgtacattaa
     1021 caatgtttaa gaaatcattt tggcatagaa ttggttaaat ataatatatt gtataacaaa
     1081 tgaaaaccag actttttttc ttattctcca cgttaacgat tttcgtattt catgcatatg
     1141 cacaaaatgc aactgacaaa tacgatacaa ttaccctgcc ccgcatggtg attggtgatg
     1201 atacgctgga acaaattgtt tatgagccgg taattatata tgccaaccgg ctggaaaaag
     1261 ccaatttgcc cgactggcaa atgcgctatc tgcgtatcgt ttatccgtat gccttgcgca
     1321 cagcgagggt agcaaaaaaa gtagatgaag aactggcgca aatggacaaa aaacgggata
     1381 aaaagaagta tttgaatgat tgcgagaaag tgctgcgtga acagtttgaa aatacattaa
     1441 aaaatttgac acgaaaacaa ggacaaatgc ttatcttgct catcaatcgg gaaacaggca
     1501 gaacagttta cgacttgctg agcgactacc gaagcggctt taaagctttc tggtggaatg
     1561 tgataggcaa aatgtatgat ttggatatga aagccgttta tgagccaaat ggaaaagata
     1621 aagaaatgga aaaatatgtg ctgtttctgg aagatgttta taaagtagat ggtacaaaat
     1681 atataataga taacgaagat gtcaatatac caaattccaa taaataatgc caaaggcaag
     1741 ccgctgaact gggacgatta caaaggtaaa gttctgctga tagtgaacac tgcatcgaag
     1801 tgcggtctga caccacagta tgatgatctg gaaaaattgt atcaggaata caaagacaga
     1861 ggttttgaaa ttatcggttg cccgagtaat gattttgcag gacaagaacc gcttgattcg
     1921 gataaggctg aagaattttg caagctcaac tatggcgtta cct
//
LOCUS       DAOQII010000334         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_51058_length_1896_cov_4.637154, whole genome shotgun sequence.
ACCESSION   DAOQII010000334 DAOQII010000000
VERSION     DAOQII010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51058_length_1896_cov_4.637154"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLF48_11685"
     CDS             complement(<1..633)
                     /locus_tag="PLF48_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HNF50035.1"
                     /translation="MAKIDARVFKGARDFLPEQMLQRERIVHLMRSVFKKYGYDPVET
                     PAIEYLNVLTGKYGDEADRLIYKLNYKTGSKDEAALHYDLTVPFSRMVAMHPDLPIPF
                     KRYQIQPVWRADRPQPHQGRFREFYQCDVDCVGSESMLIEAELIAINFEILTALNIPD
                     FLIKINNRKILNGITEYIGLDKTYVSEVCTSIDKLDKVAWTEVEKELSEKN"
     gene            complement(636..1205)
                     /locus_tag="PLF48_11690"
     CDS             complement(636..1205)
                     /locus_tag="PLF48_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF50036.1"
                     /translation="MKFSFAIIFVLSLMFFSCNSQKPTNYAGKGKYFDILTYVREDLA
                     NNIRNNCGEEKTVYLNGRHETRKIDTVNWEDELEPLLNCDINYPAWDGKFFVDSAATK
                     FANPGTMQYFYRSIDKKIPITSMTVNKDSIGRVINVVIQKSVKSFLFSHRQSIEYIPK
                     RGYSLRSEQKTNLFNSVSVNVDIKYNCKE"
     gene            complement(1365..>1896)
                     /locus_tag="PLF48_11695"
     CDS             complement(1365..>1896)
                     /locus_tag="PLF48_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNF50037.1"
                     /translation="YYFNKSYTTRIELPSLSNFDNTNDFSISFWAKTDNVAGYGTTIL
                     SDEPNVSSDRFQININYSGSPVNTNIFDYGNISGGGRLVSPASSVVFSTWEHYVFVKS
                     STGNFMKIYKNGAEIATKAGVSTKSNKSKIIVVGGSPANTEHGDQFFKGSLDDLKIFN
                     RALTATEVNQIYNAER"
BASE COUNT          575 a          354 c          341 g          626 t
ORIGIN      
        1 atttttttct gaaagttctt tttctacttc tgtccaagcc actttatcca gtttgtcaat
       61 agaagtacaa acctcagaaa cataggtttt atctaagcca atatattccg tgatgccgtt
      121 cagaattttt cggttgttga ttttaatgag aaaatccgga atgttaagtg cagtaagaat
      181 ttcaaaattg atggcaatta attctgcttc tatcagcata gattctgaac ccacacagtc
      241 cacatcgcat tgataaaact cccgaaaacg cccttgatgt ggctgtggtc tgtcggcgcg
      301 ccatacgggc tgtatctggt aacgcttgaa tggaatgggc aaatctggat gcatggctac
      361 catgcgagaa aatggaaccg ttaaatcata gtgtaaggcg gcttcatctt ttgagcctgt
      421 tttgtaattg agtttgtaaa tcagcctgtc ggcttcatcg ccgtattttc cggtcaggac
      481 attcaggtat tcgatagccg gggtctccac cggatcatag ccgtattttt taaaaacgct
      541 gcgcatgaga tgaacgatac gttcacgttg caacatctgt tcgggaagaa aatctctcgc
      601 acctttaaaa actcttgcat caatttttgc catgattact ctttacaatt atattttatg
      661 tctacattaa cacttacgct gttaaataag tttgtttttt gttcgcttct caacgaatag
      721 cctcttttag gaatatattc aatagactgc ctgtgcgaga acagaaatga tttgactgat
      781 ttttgaataa ctacattaat aacccgccca attgagtctt tattgacggt catggaggta
      841 ataggtattt ttttatctat actgcgatag aagtattgca ttgttcccgg atttgcaaat
      901 ttggtagctg cactgtccac aaaaaatttg ccatcccaag ccggataatt gatatcgcaa
      961 ttgagcaaag gttccagttc atcctcccag ttcaccgtgt ctatttttcg tgtttcatgt
     1021 ctgccgttga gataaactgt tttttcttca ccgcaattgt tgcgtatatt atttgctaaa
     1081 tcctcccgaa cataagtcaa aatatcaaaa tacttgcctt tacctgcgta attcgttggt
     1141 ttttgtgaat tacaggaaaa aaacattaga gaaaggacaa atattatggc gaatgaaaat
     1201 ttcattgggt tcaaaaataa ggaatctgcc attgataatg aaaaagatga aaataatatc
     1261 agaaagattt aacaggcata aaaaaaagag gcttccaata caggaaacct cttgctaaac
     1321 taaactacaa aaataggact aaggcttttg aacgaccata ttatttatct ttccgcatta
     1381 tagatttgat ttacttctgt tgctgttaaa gcccggttga agattttaag gtcatccaat
     1441 gaacctttaa agaattgatc tccatgttct gtgtttgcag ggctaccacc gacaacaatt
     1501 attttagatt tatttgattt agtagatact ccagcttttg tagcaatttc agcaccgttt
     1561 ttataaattt tcataaaatt acccgtgctg ctttttacaa atacataatg ttcccatgtg
     1621 ctaaatacaa cgcttgatgc tggtgaaact aaacgaccgc ctcctgaaat attgccgtaa
     1681 tcgaagatat tggtgtttac cggtgagcca gaataattga tgttgatttg aaatctgtca
     1741 ctagatacat ttggctcatc tgaaagtata gtagtaccat agcccgcaac attatcagtt
     1801 ttagcccaga aactaattga aaaatcattt gtgttatcaa aattacttaa agagggcaat
     1861 tcaattctgg tggtataact cttattaaaa taataa
//
LOCUS       DAOQII010000335         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_54095_length_1841_cov_5.828108, whole genome shotgun sequence.
ACCESSION   DAOQII010000335 DAOQII010000000
VERSION     DAOQII010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54095_length_1841_cov_5.828108"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="tilS"
                     /locus_tag="PLF48_11700"
     CDS             complement(<1..500)
                     /gene="tilS"
                     /locus_tag="PLF48_11700"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNF50038.1"
                     /translation="MSTIAQKIQTTIHNHHLMQHGAPVLVACSGGIDSMFLCEILQEL
                     NYTFGIAHVNFGLRGNESEGDAAFVKQYAEKNGILIHSIKFDTLDYKKKTGVSTQMAA
                     RELRYTWFEQIRKENGYHCIVTAHHLDDQIETVILNLAKGSGIKGIRGMLPKSGHVVR
                     PLLAIT"
     gene            complement(526..867)
                     /gene="arsC"
                     /locus_tag="PLF48_11705"
     CDS             complement(526..867)
                     /gene="arsC"
                     /locus_tag="PLF48_11705"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNF50039.1"
                     /translation="MITVYHNSRCGKSRDALKYLEERNIPYTVVEYLKDTPDEKLLQS
                     LLKKLNMKPHDLIRTKEAIYIENYKGKMLSDAEWIQAMVTHPILIERPVVVLGNKAVI
                     ARPTEKIAELF"
     gene            1245..>1841
                     /locus_tag="PLF48_11710"
     CDS             1245..>1841
                     /locus_tag="PLF48_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNF50040.1"
                     /translation="MHNLMAVSPVDGRYHSKTEKLADYFSEYALIRYRIFVEIEYFIE
                     LSAAINEIGELNESQKSALRKIYTQFNLDDALRVKEIEKVTNHDVKAVEYFIKERIKN
                     KELKKKSEFIHFGLTSQDINNTAIPLSLAGFFKNVYLAKYNELVQLLKNHAKQYAKVA
                     MLAKTHGQPASPTRLGKEWQVFIERLDKQFAYFNSIPFS"
BASE COUNT          610 a          325 c          309 g          597 t
ORIGIN      
        1 ttagtaatag ctaaaagcgg acgcacaaca tggccagatt taggcaacat gccacgtata
       61 cctttaatgc cgctgccttt tgctaaattc aaaataacag tttcaatttg gtcatccaaa
      121 tgatgggctg tgacgatgca gtgataacca ttttcttttc gtatttgctc aaaccaggtg
      181 tagcgcaatt cgcgagctgc catttgtgta gaaactccgg ttttcttctt ataatctaat
      241 gtgtcaaatt taatggagtg aattaaaatt ccattttttt cggcatactg ttttacaaat
      301 gcggcatcac cttcactttc gtttccacgc aaaccaaaat tgacatgtgc aatgccaaaa
      361 gtatagttga gttcttgtag tatttcacat aaaaacatag aatcgatacc gccactgcaa
      421 gcaactaaaa cgggagcacc atgttgcatc aagtgatggt tatgaatagt tgtctgtatt
      481 ttttgagcga tggttgacat gaaagaacaa aataataaaa tataattaaa acaactcggc
      541 aattttttca gtaggccgtg cgataacagc cttattgccc aaaacaacaa caggacgttc
      601 gatgagaatg ggatgtgtga ccattgcttg aatccattcg gcatcgctta gcattttgcc
      661 tttgtagttc tctatataga tggcttcttt agtacgaatt aaatcatgag gtttcatatt
      721 cagtttcttc aaaagagatt gcaataattt ttcgtcaggc gtatctttaa gatattccac
      781 aaccgtataa ggaatatttc tttcttccag atatttaaga gcatctctgc ttttgccaca
      841 gcggctatta tgataaactg taatcatgag ctatgtttta aactacaaat ttaggaagtt
      901 tacagctaaa agataaattc aggataacat aacaagtgag atgcagacta taggaaaaat
      961 tagtgattac tattaataat ataaacaata tacgattatt atgctctcag cttattttaa
     1021 aattctgttt tcaatattta taataaagta tgcattaatc aaattataat tcagtattat
     1081 gctacttgct tcttttatat ttatcattac attgacaaac tatactggct acattttgtt
     1141 ctgatttatg cacaaatctc acagctttct aaaaaatcac aaatacttta cccgcttaaa
     1201 tctttttgct gtttaaagaa ttgtattttt acaaaaaatc taccatgcac aatttgatgg
     1261 cagtttcgcc ggttgacggc agataccaca gcaaaactga aaaacttgcc gattattttt
     1321 cggaatatgc ccttatccgc tatcgcattt ttgtagaaat tgaatatttt attgagctgt
     1381 ctgctgcgat taatgaaata ggagaattga acgaaagcca gaaatccgct ttgcgaaaaa
     1441 tatataccca atttaatttg gatgatgctt tgcgtgttaa agaaattgag aaagtgacca
     1501 atcacgatgt aaaagcggta gagtacttta taaaagagcg tattaagaat aaagaactca
     1561 aaaagaaatc ggagtttatc cacttcggac tcacttctca ggacatcaat aatactgcca
     1621 tacctttaag tctagccggt ttttttaaaa atgtctatct tgcaaaatat aacgaacttg
     1681 ttcaattgct gaaaaatcat gcaaagcaat atgccaaagt tgctatgctg gcaaaaacac
     1741 atggacagcc cgccagccct accagattgg gtaaggaatg gcaagttttt attgaacgat
     1801 tagataaaca atttgcctat tttaattcaa ttccatttag t
//
LOCUS       DAOQII010000336         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_56813_length_1796_cov_4.596209, whole genome shotgun sequence.
ACCESSION   DAOQII010000336 DAOQII010000000
VERSION     DAOQII010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56813_length_1796_cov_4.596209"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLF48_11715"
     CDS             complement(<1..735)
                     /locus_tag="PLF48_11715"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNF50041.1"
                     /translation="MSTNKFSKTITQDPTQPAAQAMLHAIGLTDDDFKKAQVGICSTG
                     WDGNPCNMHLNALAAQLKSAVNKQNLIGLNFYTIGVSDGISMGTAGMRYSLVSREVIA
                     DSLETNAGAQYYDGIISIAGCDKNMPGVIMGMSRLNRPSLMVYGGTIAAGNYKGQALD
                     VVSAFEALGQKFAGKISDEDFKGVVENACPGAGACGGMYTANTMASAIETLGMSLPYS
                     ASNPALSEDKKKEIQAAANAIKLLLLL"
     gene            complement(785..1225)
                     /locus_tag="PLF48_11720"
     CDS             complement(785..1225)
                     /locus_tag="PLF48_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF50042.1"
                     /translation="MTTVNVYLNFNGNCEEAFLYYKSIFNTDKFQTFVKFSDMPPHEG
                     APPIPDEMKSNVMHVALRISEETVLMGSDTGGEWAPYFKQGNNFSISISTDSKEQANH
                     FFNKLSEGGTIKMPIMDTFWGEYFGMAEDKFGINWMVSHKLGEK"
     gene            complement(1236..>1796)
                     /locus_tag="PLF48_11725"
     CDS             complement(1236..>1796)
                     /locus_tag="PLF48_11725"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNF50043.1"
                     /translation="NTITGSIGIFGMIPNIRKTLNTKVGVTFDGVKTNDHANYMTLTN
                     DWDEVEMNAVQKSVEHGYSIFLNRVATGRKMDTAAVNKIARGRVWTGQDALNIGLVDE
                     LGNLNTAIKKAEELAKLKSSSVVTYPKSKTMIESLMNQFSGENESEEAKLFKTLTPLL
                     IKQKNEERLNAVNTLQYLMPFEFKLK"
BASE COUNT          479 a          365 c          323 g          629 t
ORIGIN      
        1 caatagcagt aataatttta tggcatttgc agcagcttgt atttctttct ttttgtcttc
       61 agataatgca ggattggatg cggagtaagg taaactcatg cctaaggttt cgatagcaga
      121 tgccatcgta ttggccgtgt acatgccacc gcaagcacct gcaccggggc aggcattttc
      181 aacaacacct ttgaaatctt catccgatat ttttccggca aatttttgtc ccaatgcttc
      241 gaatgcagaa accacatcta atgcttgtcc cttgtaatta cctgcggcaa tagtgccacc
      301 gtaaaccatc agagacggac ggtttaatct tgacataccc atgataactc cgggcatatt
      361 tttatcacaa cctgcaatgg aaataatgcc atcataatat tgtgctccgg cattggtttc
      421 taaagaatca gcaataacct ctcgggaaac caaagaatag cgcatgcccg cagtgcccat
      481 ggaaatacca tcacttacac caatggtata gaaatttaaa ccgattaaat tttgtttatt
      541 aacagctgat tttagttgcg cagcaagtgc attcaaatgc atattgcaag gattgccgtc
      601 ccatcctgta gagcaaatgc ctacttgtgc ttttttgaaa tcatcatctg tcagccctat
      661 ggcatgcaac atggcttgag ctgcaggttg agtaggatct tgtgtgatag tttttgaaaa
      721 tttatttgtt gacatgagtg ttatttgagt gttttaaatt taagctagca tccggtctag
      781 aattttattt ttctccaagc ttgtggctta ccatccagtt gatgccgaat ttatcttctg
      841 ccatgccaaa atattcaccc caaaaagtat ccatgattgg catcttgata gttccgccct
      901 cagataattt attaaaaaaa tgatttgctt gttctttaga gtctgttgag atgcttattg
      961 aaaaattatt gccttgctta aaataaggtg cccattctcc accggtgtcg ctgcccatca
     1021 atacggtttc ttcactgatt cttaaagcta catgcatgac gttacttttc atctcatcag
     1081 gtattggtgg agcaccttca tgtggaggca tatctgagaa ttttacaaat gtctggaatt
     1141 tatcggtgtt aaaaatgctt ttataatata aaaaagcttc ctcgcagttt ccattgaaat
     1201 ttaaatatac atttactgtt gtcatactta tttttttatt ttaatttaaa ctcaaatggc
     1261 attaaatatt gcaaggtatt tactgcattc agtcgttctt catttttttg ttttattaat
     1321 agtggtgtta gtgttttgaa aagtttggct tcttcagatt cattttcacc gctgaattga
     1381 ttcatcaaac tttcgatcat cgttttcgat tttggataag taaccacaga cgaagatttc
     1441 agttttgcca attcttccgc tttcttaatt gccgtgttta ggttgcctaa ttcgtctact
     1501 aagccaatat ttagtgcatc ctggcctgtc catactctac ctcgtgctat tttgttcaca
     1561 gcagcagtat ccatttttcg cccagttgcc acacgattta aaaatataga atatccatgc
     1621 tctacactct tttgtacggc attcatttct acttcatccc agtcgttagt gagtgtcatg
     1681 tagtttgcat ggtcatttgt ttttacaccg tcaaaagtaa ctccaacctt cgtgttgaga
     1741 gttttgcgga tattgggtat catgccaaaa atgcctatag agcctgttat cgtgtt
//
LOCUS       DAOQII010000337         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_61232_length_1729_cov_6.464755, whole genome shotgun sequence.
ACCESSION   DAOQII010000337 DAOQII010000000
VERSION     DAOQII010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61232_length_1729_cov_6.464755"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLF48_11730"
     CDS             <1..291
                     /locus_tag="PLF48_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNF50044.1"
                     /translation="IAGGIKVEDPAVDLAVVAALLSSYEDTALSIKSCFSGEVGLSGE
                     VRAVNKVEQRVSEADKMGYSKIYISKFNQKTNSTNLSISRIKTIEEFYRNLF"
     gene            293..994
                     /locus_tag="PLF48_11735"
     CDS             293..994
                     /locus_tag="PLF48_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNF50045.1"
                     /translation="MLPTTLHIALDFFRLFYPKNCAGCQTALVKNEAYICLKCLLDLP
                     YTHFEKLKDNPAEKLFYGRVNIEFAAAILFFSKHESVQHILHHIKYGEYKALGEYMGR
                     VMGERIQQDSLYAPIDLIIPVPLHPRKEHIRGYNQSKLLADGISEILKIPLADKHLIR
                     NIHTDTQTKKSRIERWENVEHAFEVRKSNQLNDKHILLVDDVLTTGATLEACAQTLLK
                     SETCKISIITLAMAH"
     gene            complement(1183..1722)
                     /gene="frr"
                     /locus_tag="PLF48_11740"
     CDS             complement(1183..1722)
                     /gene="frr"
                     /locus_tag="PLF48_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNF50046.1"
                     /translation="MDETREHMHKALKHLEEELRHVRAGKASPEMLEGIHVEYYGVPT
                     ALNQVAGIKVMDSRTLMITPFERRIIGDIERAIFQGNIGVTPQNDGENIRITMPIMTE
                     ERRKELVKQSKNHGEHTKIAVRAARKDSMEMVKAMQKDGLSEDLVKNTEQDIQHLTDE
                     HIAKVDKMLQLKEEEIMHI"
BASE COUNT          527 a          326 c          340 g          536 t
ORIGIN      
        1 attgccggag gcataaaagt agaagaccct gctgttgatc ttgcagtagt agcggctctg
       61 ctatcatctt atgaggatac ggcactcagt attaaaagtt gtttcagtgg tgaagtggga
      121 ttgagtggtg aagtgcgtgc cgtaaacaaa gtagagcaac gtgtgtctga agccgacaag
      181 atgggctatt ctaaaattta catttcaaaa ttcaaccaga aaacaaacag tacgaattta
      241 agtatcagca gaataaagac catcgaagaa ttttaccgga atttgtttta atatgctacc
      301 aaccacccta catattgccc ttgatttttt caggttgttt tatcctaaaa attgtgcagg
      361 atgccaaacg gctttggtaa aaaatgaagc ttatatctgc ttaaaatgcc tgctcgactt
      421 gccttatacc cactttgaaa aactaaaaga caatccggct gaaaaattat tttacggcag
      481 ggtgaatatt gaatttgctg ctgctatttt attcttctct aagcatgaat cggtacagca
      541 tattttgcat catatcaaat atggcgagta taaggcattg ggtgaatata tgggcagagt
      601 gatgggcgaa cgcatacagc aagattcttt atacgctccg attgacttaa ttattccggt
      661 gccacttcat cctcgcaaag agcatatccg cggatacaat caaagcaaat tgcttgcaga
      721 tggcataagt gaaatactaa aaattccttt agctgataag catttaatac gaaatataca
      781 tactgacaca cagactaaaa aaagcagaat agaacgatgg gaaaatgtag aacatgcttt
      841 tgaagtaaga aaatcgaatc aactaaatga caaacacatt ttattggttg atgatgtact
      901 gacaaccggt gctaccttag aagcttgtgc gcaaactttg ctgaaatctg aaacatgcaa
      961 gatcagcatt attacgctgg caatggcaca ttgattttat tttacaactt gagtttgggt
     1021 gaaaaaaata attgatttcg cattttatct ccaacttaac aaggaggagg aattaaaagg
     1081 atgtagttta tgataataaa accaactatt cttaaattga aaccttataa cgagttcagg
     1141 tttgtaatac ggaaatagaa aataagccct aacgattaat cactatatat gcataatttc
     1201 ctcttctttg agttggagca ttttgtctac tttggcaata tgctcatctg tgagatgctg
     1261 aatatcttgc tctgtatttt tcaccaagtc ttcgctaagt ccgtcttttt gcatggcttt
     1321 taccatctcc attgaatctt tgcgagccgc gcgcacggca attttggtat gctcgccatg
     1381 atttttagat tgttttacca actctttacg tctttcttct gtcatgatag gcatcgtgat
     1441 gcgtatgttt tcaccatcgt tttgcggagt aactcctatg tttccttgaa agatagcccg
     1501 ttcgatatca ccgataatgc ggcgttcaaa tggagtaatc atcagtgttc tggagtccat
     1561 gactttaata ccggctactt gattaagagc agttggcaca ccgtaatatt ctacatgaat
     1621 accttcgagc atttccggac tagctttgcc tgccctaacg tggcgcaatt cttcttctaa
     1681 atgcttaaga gccttgtgca tgtgctcgcg agtttcgtcc agtaaaaat
//
LOCUS       DAOQII010000338         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_63804_length_1694_cov_4.114704, whole genome shotgun sequence.
ACCESSION   DAOQII010000338 DAOQII010000000
VERSION     DAOQII010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63804_length_1694_cov_4.114704"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..688)
                     /locus_tag="PLF48_11745"
     CDS             complement(80..688)
                     /locus_tag="PLF48_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNF50047.1"
                     /translation="MKIFCVGLNYREHIVEMKHFAFPESPVIFMKPPTALLKNGQPFY
                     YPDFSQDVHYEGEIVLRIGKNGKKISEKFAHKYIDAYTLGFDFTARDIQALQKKNGWP
                     WEIAKGFDGSAFIGNFQVFNAATMPISFRIAKNGETVQQGNTSMLIFNFDSIISYISK
                     FFTLQQGDLIFTGTPQGVGPVKIGDTLEGFIGETSIVKCEVK"
     gene            923..>1694
                     /locus_tag="PLF48_11750"
     CDS             923..>1694
                     /locus_tag="PLF48_11750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNF50048.1"
                     /translation="MPTITKKKPEAKIRKVNFVFENPFPKHWYNENPIATHFMNAQHL
                     AFPDGEKFFIRSVKAFADVYKNDPELKKRVDNFIGQEGTHYAEHQKFWDIMEEQQINP
                     MRFVKYFRKTAWNGLESWARKTFIKNRFGDKLALSVTVALEHYTAMLAESGIVNKDIS
                     EKMPDEMRDLFLWHAAEEIEHKSIPFDVLKRVDDSYALRIGGMLIATWGLWYYLGVGT
                     IMLTQNDEDVKAKDIPADVALFLKKFSESFGGTLSKQYF"
BASE COUNT          580 a          288 c          287 g          539 t
ORIGIN      
        1 tatatataaa gtttctcgtc aaatactatc gtcaatcaaa tttatattta caaataactt
       61 atctctaatc accaatatct cattttacct cacatttcac gatactagtt tctccaataa
      121 atccttctaa ggtatcacct atttttacgg gacctacgcc ttgtggtgta ccggtaaata
      181 tcaaatcgcc ttgttgtagg gtgaaaaatt tagaaatata actgataatg gaatcgaaat
      241 taaaaatcaa catcgaagta tttccttgtt gaacagtttc accgtttttg gctattcgaa
      301 atgaaatggg catagtagca gcattaaata cttgaaaatt gcctataaaa gcagagccgt
      361 caaaaccttt ggcaatctcc catggccagc catttttctt ttgtaatgct tgaatatctc
      421 ttgctgtaaa atcaaatccc aaagtgtatg catcaatata tttgtgtgca aatttctcgc
      481 ttatcttttt tccatttttg ccaatgcgca atacgatttc tccttcataa tgcacgtcct
      541 gagaaaaatc cggataataa aaaggttgac cattcttgag taaagctgtt ggcggtttca
      601 taaatatcac cgggctttcc ggaaacgcaa aatgtttcat ctctacaata tgttctctgt
      661 aattcaagcc tacgcaaaat attttcatac atcaaaatta ttgcatgaaa ttgagaaaaa
      721 aatcagacat aataaaacgg tacaaattat ataaacagca tatttcaatc aaaaaaatca
      781 gcttaacatg aaaaaccaag ttaaaatcag tcaaaatcaa aaatttacat ttgtaaagca
      841 ataagcgatg taactgctaa ttatcttaag ataattgtcc tttgaaatga tgtagtttac
      901 ttttgattaa acaataagtt atatgccaac aataaccaag aagaaacctg aagcaaaaat
      961 tcgtaaagta aattttgtat ttgaaaatcc atttccaaaa cattggtata acgaaaatcc
     1021 tatagctaca cattttatga atgcacagca tctggctttc ccggatggtg aaaaattttt
     1081 cataagaagt gtgaaagcat ttgctgatgt atataagaat gaccctgaat tgaaaaaacg
     1141 tgttgacaat tttatcggac aagaaggtac gcattatgca gaacatcaaa agttttggga
     1201 catcatggaa gaacagcaaa tcaatccgat gcgctttgtc aaatattttc gcaaaactgc
     1261 ttggaatggt ttagaaagct gggcacgtaa aacatttata aaaaacaggt tcggcgacaa
     1321 actggctctt agtgtaactg ttgctcttga acattatacc gctatgctgg ctgaaagtgg
     1381 catcgtcaac aaggatattt ctgaaaaaat gcccgatgag atgcgcgact tatttttgtg
     1441 gcatgctgct gaagaaattg agcataaatc tattcctttt gatgtattaa aacgtgtaga
     1501 tgacagctat gctttgcgta ttggtggtat gttgattgct acttggggac tttggtatta
     1561 tttgggtgtc ggtacaatta tgcttacaca aaatgatgaa gatgtgaaag caaaagatat
     1621 tccggctgat gtagccttat ttttaaagaa attttctgag agttttggcg gtactttatc
     1681 taagcaatac ttcc
//
LOCUS       DAOQII010000339         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_64900_length_1680_cov_5.148923, whole genome shotgun sequence.
ACCESSION   DAOQII010000339 DAOQII010000000
VERSION     DAOQII010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64900_length_1680_cov_5.148923"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PLF48_11755"
     CDS             complement(<1..1266)
                     /locus_tag="PLF48_11755"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNF50049.1"
                     /translation="MNYEETINYLYKQLPMFSRIGDAAIKKDLTNTLLLCTALDHPQD
                     KIKTIHIAGTNGKGSCSHLLASVMQENGYKTGLYTSPHLKDFRERIKVNGKVCPENFV
                     IDFVEKIKPEIERIQPSFFEITVAMAFDYFAKEKVDIAIIETGLGGRLDSTNVIHPVL
                     SVITNISLDHTALLGNTLEEIAFEKGGIIKENTPVVIGKQNVNTDGIFEKQANEKQAK
                     IYFSANAFSFGKIDVHDKYSDFYFHGETDFFIRSDLRAAYQQENIRTVYMVLQVLENE
                     KIMQLNAKKNAAGFLHCTTNTQFQGRWQTLSNNPKIIADVAHNEDGVKNMLEQLQDEK
                     YKQLHIVYGAVKDKDVDAILKLLPKSAHYYFTEPPVPRKLEATHLAEKAKKFSLKGKI
                     YAHPKLALAAAKATAKESDLILVTGSFFVL"
     gene            complement(1269..>1680)
                     /locus_tag="PLF48_11760"
     CDS             complement(1269..>1680)
                     /locus_tag="PLF48_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNF50050.1"
                     /translation="TDDEHILRQMSLMGEYIGMAFQIKDDLFDFGEADIGKPRGIDIK
                     ERKMTLPLIYTLNNCSAQEKKWIINIVKNHNEDKAKVNELIRFINTHGGIAYTEKKML
                     EYKQKAFDIMLTMKENTARQSLMDLINYITERNY"
BASE COUNT          470 a          330 c          271 g          609 t
ORIGIN      
        1 caatacaaaa aaactgccgg ttaccagtat taaatcgctt tcttttgcag tagctttagc
       61 agcagctaat gccaattttg gatgcgcata aattttacct tttaatgaaa atttttttgc
      121 tttctccgct aaatgagtag cctctaattt tctgggaaca ggcggctcgg taaaatagta
      181 atgtgcactt ttaggtaata attttagaat agcgtcaaca tctttatctt taactgcacc
      241 ataaacaata tgcaattgct tatacttttc atcttgcaat tgttccagca tattttttac
      301 gccgtcttca ttatgtgcca catctgcaat gatttttgga ttattcgata aagtttgcca
      361 gcgtccttga aattgtgtat tggttgtgca atgcaaaaat cccgctgcat ttttcttagc
      421 atttaattgc atgatttttt cattttccaa gacttgcaaa accatataaa cagtgcgtat
      481 attttcttgc tgataggcag ctctcaaatc actgcgtata aaaaaatccg tttccccatg
      541 aaaataaaaa tcggaatact tatcgtgtac atcaattttg ccaaaagaaa aagcattagc
      601 tgaaaaataa atttttgcct gtttttcatt tgcctgtttt tcaaaaatcc catccgtatt
      661 tacattctgt tttccaatga ctacaggggt gttttctttg ataatacctc ctttttcaaa
      721 ggcaatttct tccaacgtat tccccaataa tgcagtatga tctaatgaaa tattggtgat
      781 aacagataaa acaggatgga tgacatttgt agaatctaag cgaccaccca atccggtttc
      841 tatgatggca atatctactt tttctttagc aaaataatcg aatgccatgg ctaccgtaat
      901 ctcaaaaaaa gaaggttgta ttcgctcaat ctccggtttt attttttcta caaaatctat
      961 tacaaaattt tcaggacaaa ccttgccgtt cacttttata cgttctctga aatctttcag
     1021 atgtggggaa gtatataatc cggttttata tccgttttcc tgcatcaccg aagccagcaa
     1081 atgcgagcaa ctgcctttgc cgttagtgcc tgctatatgg atagttttta ttttgtcttg
     1141 cggatgatct aaagctgtac ataacaacag cgtattagtc aaatcttttt tgatggcagc
     1201 atctcctatg cgcgaaaaca tgggcaactg cttgtacaga tagtttattg tttcttcgta
     1261 attcaaactt aatagttgcg ttctgttata tagttgatta aatccatgag gctttgtctt
     1321 gccgtatttt ctttcatagt aagcataatg tcaaatgcct tttgtttgta ttcgagcatt
     1381 ttcttttccg tgtaggctat gccgccatgt gtatttataa agcgtattaa ctcattcact
     1441 tttgctttgt cttcattatg atttttcacg atattgataa tccatttttt ttcctgagca
     1501 gagcaattat tcagcgtata aatgagcggc aacgtcatct tccgttcttt aatatcgata
     1561 cctctgggct tgccgatgtc tgcctcgcca aaatcaaata aatcatcttt tatctggaaa
     1621 gccatgccga tatactcgcc catcaaactc atttgtcgca atatatgttc atcgtcggtt
//
LOCUS       DAOQII010000340         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_67180_length_1651_cov_4.200501, whole genome shotgun sequence.
ACCESSION   DAOQII010000340 DAOQII010000000
VERSION     DAOQII010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67180_length_1651_cov_4.200501"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLF48_11765"
     CDS             complement(<1..269)
                     /locus_tag="PLF48_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF50051.1"
                     /translation="MGKFYFFSAVILLTFSLFSCKRIDRCERDFNTDTIDTVQKICFG
                     SCSREWKDEPILNTIVSEKPDIFIYLGDNVYIDSEKKRDFERKYF"
     gene            complement(262..1284)
                     /locus_tag="PLF48_11770"
     CDS             complement(262..1284)
                     /locus_tag="PLF48_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNF50052.1"
                     /translation="MQHFKFIQNNLFAFTKIRNGETKIGEKIQLIEETSQWEKEIRKS
                     KARFVVFGICESIGVQANFGIGGTETAFHSFLQAFCNVQQNQFLQGESILLLGNFDFS
                     PILKKTKDASVEYLRDLVNVIDEEVSFLVEAIVAHNKVPLIIGGGHNNAYGNIKGSAK
                     GMHRQGKMEQINVLNFDAHADFRPLEGRHSGNGFHYAMHDNFLHQYAMLGLHENYMTQ
                     EMWGRIKKNDSILPIFFEDIAIRKKYAGKKAIELVLQFLDKTPCGVEIDLDCIENVLS
                     SAMTPSGFSVTEIRKYLYQFQSKIKPVYLHICEGAEVLSSGKTQPTIGKLITYLVTDF
                     IKNYNG"
     gene            complement(1286..>1651)
                     /locus_tag="PLF48_11775"
     CDS             complement(1286..>1651)
                     /locus_tag="PLF48_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF50053.1"
                     /translation="TLLPSCSFYLRMHYAPARILIEHNAAIALASDFNPGSSPSGNMN
                     FIIALACIQMKMLPEEAINAATLNAAYAMEIQDIMGSVSKGKKANLIITKKLSSLAFL
                     PYAFGSNLIDKIILNGKFI"
BASE COUNT          497 a          324 c          290 g          540 t
ORIGIN      
        1 ttgaaatatt tccgttcgaa atctcttttc ttttccgagt ctatataaac attgtcaccc
       61 agataaataa aaatatccgg tttttcgctg acaatcgtgt ttagaattgg ttcatctttc
      121 cactcacggc tgcacgagcc aaaacaaatt ttttgtaccg tatcaatcgt atccgtatta
      181 aaatcgcgtt cgcaacggtc gatgcgtttg caggaaaaca agctaaatgt cagtagaata
      241 acagccgaaa agaaataaaa tttacccatt gtaattttta ataaaatcgg taaccaaata
      301 ggtgattagt ttgccaattg ttggctgtgt tttcccgctt gaaagtactt ctgctccttc
      361 gcaaatatgt aaatagactg gcttaatttt gctctgaaat tggtacaagt attttctgat
      421 ttctgtcact gaaaatccgg aaggagtcat ggcactgctt agcacatttt caatacaatc
      481 caaatcgatt tctacaccgc aaggagtttt atccaaaaat tgcaagacga gttctatagc
      541 ttttttaccc gcatattttt tgcggatagc aatatcttca aaaaaaatgg gcaatatact
      601 gtcgttcttt tttattcgcc cccacatttc ttgtgtcata taattttcgt gcaagcccag
      661 catggcatac tgatgtaaaa aattatcatg catggcatag tgaaaaccat taccggaatg
      721 ccggccctcc aatggtctga aatctgcatg agcatcaaaa ttgagtacat taatttgttc
      781 catcttgcct tggcggtgca ttccctttgc actccctttt atattgccgt atgcattatt
      841 atgtcctcca ccaataatga gcggcacttt attatgagcc acaatagctt ctaccaaaaa
      901 acttacttct tcatctatca catttaccaa atcacgcaga tattcaactg atgcatcttt
      961 tgttttttta agtataggtg aaaagtcgaa attacctaac aacaatatag attcgccttg
     1021 caaaaactgg ttttgttgaa cattgcaaaa agcctgtaaa aaggaatgaa aggctgtttc
     1081 ggtgccacca ataccgaagt tggcttgcac acctatggat tcacaaatac caaataccac
     1141 aaaacgggct ttcgattttc tgatttcttt ttcccattgt gaggtttctt caatcaactg
     1201 aattttttcg ccaatttttg tttcaccatt tctgatttta gtaaaagcga acaagttgtt
     1261 ttgaataaat ttgaaatgct gcatgttaaa taaattttcc gttgaggatt attttgtcaa
     1321 ttaaattaga accaaaggcg taaggcaaga atgctaagga agaaagcttt tttgtaataa
     1381 tgagattggc ttttttacct ttagaaacag agcccattat atcttgaatc tccatggcat
     1441 aagcagcatt cagtgtagcg gcattgatgg cttcttcggg cagcattttc atttgtatgc
     1501 atgccagtgc tataataaaa ttcatattac cgctcggcga tgagcccgga ttaaaatcac
     1561 ttgccagtgc aatagcggca ttgtgttcaa tcaatattct ggctggtgcg tagtgcatgc
     1621 gcaaataaaa ggaacatgat ggcaaaagtg t
//
LOCUS       DAOQII010000341         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_70654_length_1609_cov_3.406049, whole genome shotgun sequence.
ACCESSION   DAOQII010000341 DAOQII010000000
VERSION     DAOQII010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70654_length_1609_cov_3.406049"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLF48_11780"
     CDS             complement(<1..1136)
                     /locus_tag="PLF48_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF50054.1"
                     /translation="MLRVAAILALFSFLCHSSLAQQPAFDFKQLSNLKSKSFVLHADT
                     LELDSLSLFPNSITIRNLANNTLLSNEIYRYDAAENRLIFLPDNRPDSIMLSYRATPV
                     NFYSSYQHKSFNRIRHTDSTLQQYYYFYQPQQVEKNAFIDFSGLNYSGNFARGIQFGS
                     NQDLTLNSNLDLRLSGKISNNIEITAALSDNNIPLQPDGTTQQIQDFDKVYIQLKKDP
                     HIITAGDFTVKNREGYFMRFVKQMQGLWYENKFVFKNKLKLQTSSAIAVSKGKFGRNI
                     FQGAEGNQGPYRLIGNNNETFIIVLAGSEKVYIDGKLLQRGELNDYIMDYNSGELVFM
                     PRYLITKDSRIVVEFEYTDRNYLRTNANEAIRLEYKNLTAYTQL"
     gene            complement(1193..1609)
                     /locus_tag="PLF48_11785"
     CDS             complement(1193..1609)
                     /locus_tag="PLF48_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNF50055.1"
                     /translation="MDSLSLEAINAQAKNTMMEHLGIEVTEIGDDYIKAKMPVDHRTH
                     QPMGLLHGGAHVVLAESLGSMSANFCLDITKEYALGLDINSNHIRSVRDGYVYGTARP
                     IHIGSKTQVWEIKVTDENDNLLNISRLTMIVLQHKV"
BASE COUNT          481 a          301 c          299 g          528 t
ORIGIN      
        1 taaagttgcg tgtatgcggt caggtttttg tattccaatc ttatagcttc atttgcattc
       61 gttctcaagt aatttctatc ggtgtattca aattccacaa caattctcga atcttttgta
      121 attaaataac gaggcataaa aaccaattca ccgctattgt aatccatgat ataatcgttg
      181 agttcgccac gttgtaacag tttcccgtct atgtatactt tctcacttcc ggctaatacg
      241 atgataaaag tttcgttatt atttccaatt aagcggtatg gtccttgatt gccttctgcc
      301 ccttgaaaaa tattgcgacc aaatttgcct ttactaaccg caatcgctga tgaggtttgc
      361 agttttaatt tattcttgaa cacaaattta ttttcatacc acaacccttg catttgcttg
      421 acgaaacgca taaaataacc ctctctgttt tttaccgtaa aatcgcctgc ggtaatgatg
      481 tgcggatctt tttttagttg gatatagact ttgtcaaaat cctggatttg ctgtgtagtg
      541 ccatcgggtt gaagtggaat attattatca gaaagtgctg ctgttatttc aatattattg
      601 ctgatttttc cgctcaggcg caaatctaaa ttggagttca gcgttaaatc ctgattagag
      661 ccaaactgaa taccgcgcgc aaaattaccg ctgtaattca atccgctgaa atcaataaat
      721 gcattctttt ctacttgttg tggttggtaa aaatagtagt attgttgtaa ggtagaatca
      781 gtatgccgta tgcgattgaa ggatttgtgt tgatatgacg aataaaaatt tacaggcgta
      841 gcacgataac taagcattat ggaatccggt ctgttatccg gcaaaaagat aagccggttt
      901 tcagccgcat catatcgata aatttcattt gacagtagtg tattattggc taaatttcta
      961 atcgtaattg aattgggaaa caagctcaaa ctatccaatt caagcgtatc agcatgaagt
     1021 acaaaggact tggatttcag attagaaagt tgtttaaaat caaaagcagg ctgttgtgca
     1081 agcgatgaat ggcacaaaaa agagaataag gctaatattg cagcaacacg aagcaacaga
     1141 tgtttatagg taaaggtaaa aagaaagcgt ttattgtgtg taaaaagaaa aattaaactt
     1201 tatgctgcaa aacaatcatc gtcagccggg aaatattcag taaattatca ttttcatctg
     1261 tcacttttat ttcccaaact tgagtttttg aaccgatatg aatcggtcgg gctgttccgt
     1321 atacataccc atctcttaca cttcggatgt gattagagtt gatatctaat cccagtgcat
     1381 attctttggt gatatccaga caaaaattgg cactcatgct gcctaaagat tctgccaaaa
     1441 ccacatgcgc acctccatgc agtaatccca tcggctggtg tgtgcgatgg tcaaccggca
     1501 ttttcgcttt gatataatca tcgccaattt cagttacttc aatacccaga tgttccatca
     1561 ttgtattttt ggcttgcgca tttatcgctt caagagaaag gctatctat
//
LOCUS       DAOQII010000342         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0630
            NODE_72624_length_1586_cov_4.679295, whole genome shotgun sequence.
ACCESSION   DAOQII010000342 DAOQII010000000
VERSION     DAOQII010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810262
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,360
            CDSs (total)                      :: 2,339
            Genes (coding)                    :: 2,330
            CDSs (with protein)               :: 2,330
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 4 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72624_length_1586_cov_4.679295"
                     /isolate="AMR_MDS_0630"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..280)
                     /locus_tag="PLF48_11790"
     CDS             complement(53..280)
                     /locus_tag="PLF48_11790"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNF50056.1"
                     /translation="MDKPQTPDADIKKTNQWLLYTGMAFEMFMTIGIFAFLGNFLDKK
                     LNTAPFLLIFMLLIGLGIAFYRVMLAVKKMK"
     gene            complement(258..668)
                     /locus_tag="PLF48_11795"
     CDS             complement(258..668)
                     /locus_tag="PLF48_11795"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNF50057.1"
                     /translation="MFSKNGIKSNGDSLVANRITEGTTVIGDINAQADLRVDGRINGN
                     ILCNAKVIVGNSSQIAGNIKCTDLTIEGKLKGNIEVSGVLFFRSSAHFEGDVNYKKLI
                     VEEGAQISGSLTNTSGMSKSATQSTVQNGQTTNA"
     gene            complement(730..>1586)
                     /locus_tag="PLF48_11800"
     CDS             complement(730..>1586)
                     /locus_tag="PLF48_11800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF50058.1"
                     /translation="VVLKKWNWQRLKEILGNVYRFQVIDEDHFDVKFVFVLKPLNIIV
                     AVGLLLFFFTMLNFFLIAYTPLKQYIPGYGSTAGRKNLINLNMKADQLESDLHSGDKY
                     IKNLQNILNDKVVVDVVKKDMPKVNGDTGMLSMKTDDEAHFIQEMEKGLQNSALNASI
                     QESKQSVLSSLSLFRPTKGKLISAFNPQTGIEMAAQKDEQVLSPVSGIVINEMPLSGA
                     IIIQTENQLVCIVKNLSAVSKKTGNFVQKGDVIGVAGFDKTKKTSSCTLELWYKGQPL
                     DAGKYLHE"
BASE COUNT          456 a          309 c          267 g          554 t
ORIGIN      
        1 aaaatggtaa cgattagcca gatgaaaaac caaattttgt acattgaaat acttatttca
       61 tcttttttac agctaacatg acccggtaaa aggcaattcc caaaccgatt agcaacataa
      121 aaattagcaa aaatggagca gtatttagtt tcttgtctaa aaaattaccc agaaaggcaa
      181 aaattccgat agtcataaac atctcaaacg ccatacccgt atagagcagc cattgattgg
      241 tttttttgat atcagcatca ggcgtttgtg gtttgtccat tttgtactgt tgattgggta
      301 gctgattttg acattcctga tgtattggtg agtgaacctg aaatttgagc accttcttct
      361 acgataagtt ttttgtaatt cacatcacct tcaaagtggg cagaagatct gaaaaataat
      421 actccactta cttctatatt acctttcagt tttccttcaa tagtcaaatc ggtgcatttt
      481 atattgcccg ctatctgaga agaattgcct acaattactt tggcattaca taaaatattg
      541 ccgttgattc tgccgtctac tcgcaaatca gcctgtgcat ttatatctcc gattaccgtt
      601 gtgccttcgg ttattctgtt tgctactaat gaatcaccat tgctttttat accgtttttt
      661 gaaaacatac gcttgaatta atgattatgg tctaaatggt ttataatgaa tgtttaatgt
      721 ttcttctatt tattcgtgta aatattttcc tgcatccagt ggttgtcctt tataccacaa
      781 ctccagtgtg caagatgatg ttttttttgt tttgtcaaac cctgctactc ctataacatc
      841 acccttttgt acaaaattac ccgttttttt tgaaactgcg gatagatttt tcactatgca
      901 aaccagttga ttttcagttt gtatgataat tgcacctgat aagggcattt cattgataac
      961 gatgcccgat accggtgata agacttgttc gtctttttga gccgccatct cgatacctgt
     1021 ttgtggatta aatgcactga tcagcttgcc tttggtagga cgaaacagcg ataaggaaga
     1081 aagcaccgat tgtttactct cttgaataga agcattaagt gcagaatttt gcagtccttt
     1141 ttccatttcc tgaataaaat gagcttcatc atctgttttc atactgagca tacccgtatc
     1201 gccgtttacc ttgggcatat cttttttgac aacatctaca acgactttgt cgtttaatat
     1261 attttgtaaa tttttaatgt atttatctcc ggaatgtaag tcactttcca gctggtcggc
     1321 tttcatgttc agatttatca ggttttttct gcccgctgta gaaccatagc cgggtatata
     1381 ttgttttagc ggagtatagg cgattaagaa gaaattgagc atcgtaaaaa agaataaaag
     1441 caagccgact gctacaataa tattaagcgg tttcagtaca aaaacgaact ttacatcaaa
     1501 atgatcctca tcaatcactt gaaaacggta cacatttccg agtatctctt ttagccgctg
     1561 ccagttccat tttttcaaca caacac
//
